LOCUS       DAJVPT010000001        95068 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVPT010000001 DAJVPT010000000
VERSION     DAJVPT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(13..1263)
                     /gene="icd"
                     /locus_tag="OQM47_000001"
     CDS             complement(13..1263)
                     /gene="icd"
                     /locus_tag="OQM47_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1478310.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1435..2088
                     /gene="rluE"
                     /locus_tag="OQM47_000002"
     CDS             1435..2088
                     /gene="rluE"
                     /locus_tag="OQM47_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1478311.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2098..2559
                     /gene="nudJ"
                     /locus_tag="OQM47_000003"
     CDS             2098..2559
                     /gene="nudJ"
                     /locus_tag="OQM47_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1478312.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2613..3719
                     /gene="mnmA"
                     /locus_tag="OQM47_000004"
     CDS             2613..3719
                     /gene="mnmA"
                     /locus_tag="OQM47_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1478313.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3755..4396
                     /gene="hflD"
                     /locus_tag="OQM47_000005"
     CDS             3755..4396
                     /gene="hflD"
                     /locus_tag="OQM47_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1478314.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4400..5770
                     /gene="purB"
                     /locus_tag="OQM47_000006"
     CDS             4400..5770
                     /gene="purB"
                     /locus_tag="OQM47_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1478315.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5939..6610
                     /gene="phoP"
                     /locus_tag="OQM47_000007"
     CDS             5939..6610
                     /gene="phoP"
                     /locus_tag="OQM47_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1478316.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6610..8070
                     /gene="phoQ"
                     /locus_tag="OQM47_000008"
     CDS             6610..8070
                     /gene="phoQ"
                     /locus_tag="OQM47_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1478317.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8867..9148)
                     /locus_tag="OQM47_000009"
     CDS             complement(8867..9148)
                     /locus_tag="OQM47_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478318.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9162..10823)
                     /locus_tag="OQM47_000010"
     CDS             complement(9162..10823)
                     /locus_tag="OQM47_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1478319.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10807..11133)
                     /locus_tag="OQM47_000011"
     CDS             complement(10807..11133)
                     /locus_tag="OQM47_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1478320.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLEQYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11189..11371)
                     /locus_tag="OQM47_000012"
     CDS             complement(11189..11371)
                     /locus_tag="OQM47_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478321.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11355..11795)
                     /locus_tag="OQM47_000013"
     CDS             complement(11355..11795)
                     /locus_tag="OQM47_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1478322.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11795..12091)
                     /locus_tag="OQM47_000014"
     CDS             complement(11795..12091)
                     /locus_tag="OQM47_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1478323.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12088..12426)
                     /locus_tag="OQM47_000015"
     CDS             complement(12088..12426)
                     /locus_tag="OQM47_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1478324.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12423..13634)
                     /locus_tag="OQM47_000016"
     CDS             complement(12423..13634)
                     /locus_tag="OQM47_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1478325.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13636..14208)
                     /locus_tag="OQM47_000017"
     CDS             complement(13636..14208)
                     /locus_tag="OQM47_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1478326.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14248..15405)
                     /locus_tag="OQM47_000018"
                     /pseudo
     CDS             complement(14248..15405)
                     /locus_tag="OQM47_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15706..15930)
                     /locus_tag="OQM47_000019"
     CDS             complement(15706..15930)
                     /locus_tag="OQM47_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478327.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16072..16233)
                     /locus_tag="OQM47_000020"
     CDS             complement(16072..16233)
                     /locus_tag="OQM47_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1478328.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16243..16548)
                     /locus_tag="OQM47_000021"
     CDS             complement(16243..16548)
                     /locus_tag="OQM47_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478329.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16545..16823)
                     /locus_tag="OQM47_000022"
     CDS             complement(16545..16823)
                     /locus_tag="OQM47_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478330.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17112..18860)
                     /locus_tag="OQM47_000023"
     CDS             complement(17112..18860)
                     /locus_tag="OQM47_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1478331.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18857..19156)
                     /locus_tag="OQM47_000024"
     CDS             complement(18857..19156)
                     /locus_tag="OQM47_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1478332.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19162..19395)
                     /locus_tag="OQM47_000025"
     CDS             complement(19162..19395)
                     /locus_tag="OQM47_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478333.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19397..19617)
                     /locus_tag="OQM47_000026"
                     /pseudo
     CDS             complement(19397..19617)
                     /locus_tag="OQM47_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19610..19831)
                     /locus_tag="OQM47_000027"
     CDS             complement(19610..19831)
                     /locus_tag="OQM47_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478334.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19821..20021)
                     /locus_tag="OQM47_000028"
     CDS             complement(19821..20021)
                     /locus_tag="OQM47_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1478335.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20151..20330
                     /locus_tag="OQM47_000029"
     CDS             20151..20330
                     /locus_tag="OQM47_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478336.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20455..20784
                     /locus_tag="OQM47_000030"
     CDS             20455..20784
                     /locus_tag="OQM47_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478337.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20834..21022)
                     /locus_tag="OQM47_000031"
     CDS             complement(20834..21022)
                     /locus_tag="OQM47_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1478338.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21397..22626)
                     /locus_tag="OQM47_000032"
     CDS             complement(21397..22626)
                     /locus_tag="OQM47_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1478339.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22875..23996
                     /gene="roxA"
                     /locus_tag="OQM47_000033"
                     /gene_synonym="ycfD"
     CDS             22875..23996
                     /gene="roxA"
                     /locus_tag="OQM47_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1478340.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24045..25271)
                     /gene="pepT"
                     /locus_tag="OQM47_000034"
     CDS             complement(24045..25271)
                     /gene="pepT"
                     /locus_tag="OQM47_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1478341.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25521..26657
                     /gene="potA"
                     /locus_tag="OQM47_000035"
     CDS             25521..26657
                     /gene="potA"
                     /locus_tag="OQM47_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1478342.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26641..27498
                     /gene="potB"
                     /locus_tag="OQM47_000036"
     CDS             26641..27498
                     /gene="potB"
                     /locus_tag="OQM47_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1478343.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27495..28289
                     /gene="potC"
                     /locus_tag="OQM47_000037"
     CDS             27495..28289
                     /gene="potC"
                     /locus_tag="OQM47_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1478344.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28286..29332
                     /gene="potD"
                     /locus_tag="OQM47_000038"
     CDS             28286..29332
                     /gene="potD"
                     /locus_tag="OQM47_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1478345.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29488..30309)
                     /gene="cobB"
                     /locus_tag="OQM47_000039"
     CDS             complement(29488..30309)
                     /gene="cobB"
                     /locus_tag="OQM47_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1478346.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30325..31236)
                     /gene="nagK"
                     /locus_tag="OQM47_000040"
     CDS             complement(30325..31236)
                     /gene="nagK"
                     /locus_tag="OQM47_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1478347.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31265..32509)
                     /gene="lolE"
                     /locus_tag="OQM47_000041"
     CDS             complement(31265..32509)
                     /gene="lolE"
                     /locus_tag="OQM47_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1478348.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32509..33210)
                     /gene="lolD"
                     /locus_tag="OQM47_000042"
     CDS             complement(32509..33210)
                     /gene="lolD"
                     /locus_tag="OQM47_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1478349.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33203..34402)
                     /gene="lolC"
                     /locus_tag="OQM47_000043"
     CDS             complement(33203..34402)
                     /gene="lolC"
                     /locus_tag="OQM47_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1478350.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34664..35737
                     /locus_tag="OQM47_000044"
     CDS             34664..35737
                     /locus_tag="OQM47_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1478351.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35865..39311
                     /gene="mfd"
                     /locus_tag="OQM47_000045"
     CDS             35865..39311
                     /gene="mfd"
                     /locus_tag="OQM47_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1478352.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39454..40416
                     /gene="ldtC"
                     /locus_tag="OQM47_000046"
     CDS             39454..40416
                     /gene="ldtC"
                     /locus_tag="OQM47_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1478353.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40499..40711)
                     /locus_tag="OQM47_000047"
     CDS             complement(40499..40711)
                     /locus_tag="OQM47_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1478354.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40996..41628
                     /gene="comR"
                     /locus_tag="OQM47_000048"
     CDS             40996..41628
                     /gene="comR"
                     /locus_tag="OQM47_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1478355.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41690..42229)
                     /locus_tag="OQM47_000049"
     CDS             complement(41690..42229)
                     /locus_tag="OQM47_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1478356.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42456..43760)
                     /gene="ndh"
                     /locus_tag="OQM47_000050"
     CDS             complement(42456..43760)
                     /gene="ndh"
                     /locus_tag="OQM47_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1478357.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44168..44710)
                     /locus_tag="OQM47_000051"
     CDS             complement(44168..44710)
                     /locus_tag="OQM47_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1478358.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44733..45758)
                     /gene="nagZ"
                     /locus_tag="OQM47_000052"
     CDS             complement(44733..45758)
                     /gene="nagZ"
                     /locus_tag="OQM47_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1478359.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45769..46593)
                     /gene="thiK"
                     /locus_tag="OQM47_000053"
     CDS             complement(45769..46593)
                     /gene="thiK"
                     /locus_tag="OQM47_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1478360.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46574..47215)
                     /gene="lpoB"
                     /locus_tag="OQM47_000054"
     CDS             complement(46574..47215)
                     /gene="lpoB"
                     /locus_tag="OQM47_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1478361.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47230..47607)
                     /locus_tag="OQM47_000055"
     CDS             complement(47230..47607)
                     /locus_tag="OQM47_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1478362.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47610..47969)
                     /gene="hinT"
                     /locus_tag="OQM47_000056"
     CDS             complement(47610..47969)
                     /gene="hinT"
                     /locus_tag="OQM47_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1478363.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48308..50493
                     /gene="fhuE"
                     /locus_tag="OQM47_000057"
                     /pseudo
     CDS             48308..50493
                     /gene="fhuE"
                     /locus_tag="OQM47_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50553..51986)
                     /gene="ptsG"
                     /locus_tag="OQM47_000058"
     CDS             complement(50553..51986)
                     /gene="ptsG"
                     /locus_tag="OQM47_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1478364.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52281..53078)
                     /locus_tag="OQM47_000059"
     CDS             complement(52281..53078)
                     /locus_tag="OQM47_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1478365.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53089..54093)
                     /gene="holB"
                     /locus_tag="OQM47_000060"
     CDS             complement(53089..54093)
                     /gene="holB"
                     /locus_tag="OQM47_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1478366.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54090..54731)
                     /locus_tag="OQM47_000061"
     CDS             complement(54090..54731)
                     /locus_tag="OQM47_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1478367.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54721..55743)
                     /gene="yceG"
                     /locus_tag="OQM47_000062"
     CDS             complement(54721..55743)
                     /gene="yceG"
                     /locus_tag="OQM47_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1478368.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55746..56555)
                     /gene="pabC"
                     /locus_tag="OQM47_000063"
     CDS             complement(55746..56555)
                     /gene="pabC"
                     /locus_tag="OQM47_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1478369.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56675..57916)
                     /gene="fabF"
                     /locus_tag="OQM47_000064"
     CDS             complement(56675..57916)
                     /gene="fabF"
                     /locus_tag="OQM47_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1478370.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58003..58239)
                     /gene="acpP"
                     /locus_tag="OQM47_000065"
     CDS             complement(58003..58239)
                     /gene="acpP"
                     /locus_tag="OQM47_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1478371.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58450..59184)
                     /gene="fabG"
                     /locus_tag="OQM47_000066"
     CDS             complement(58450..59184)
                     /gene="fabG"
                     /locus_tag="OQM47_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1478372.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59197..60126)
                     /gene="fabD"
                     /locus_tag="OQM47_000067"
     CDS             complement(59197..60126)
                     /gene="fabD"
                     /locus_tag="OQM47_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1478373.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60142..61095)
                     /gene="fabH"
                     /locus_tag="OQM47_000068"
     CDS             complement(60142..61095)
                     /gene="fabH"
                     /locus_tag="OQM47_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1478374.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61163..62233)
                     /gene="plsX"
                     /locus_tag="OQM47_000069"
     CDS             complement(61163..62233)
                     /gene="plsX"
                     /locus_tag="OQM47_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1478375.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62314..62487)
                     /gene="rpmF"
                     /locus_tag="OQM47_000070"
     CDS             complement(62314..62487)
                     /gene="rpmF"
                     /locus_tag="OQM47_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1478376.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62539..63060)
                     /gene="yceD"
                     /locus_tag="OQM47_000071"
     CDS             complement(62539..63060)
                     /gene="yceD"
                     /locus_tag="OQM47_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1478377.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63259..63843
                     /gene="yceF"
                     /locus_tag="OQM47_000072"
     CDS             63259..63843
                     /gene="yceF"
                     /locus_tag="OQM47_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1478378.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63955..64914)
                     /gene="rluC"
                     /locus_tag="OQM47_000073"
     CDS             complement(63955..64914)
                     /gene="rluC"
                     /locus_tag="OQM47_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1478379.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65487..68678
                     /gene="rne"
                     /locus_tag="OQM47_000074"
     CDS             65487..68678
                     /gene="rne"
                     /locus_tag="OQM47_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1478380.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68875..69817)
                     /gene="flgL"
                     /locus_tag="OQM47_000075"
                     /pseudo
     CDS             complement(68875..69817)
                     /gene="flgL"
                     /locus_tag="OQM47_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69829..71471)
                     /gene="flgK"
                     /locus_tag="OQM47_000076"
                     /pseudo
     CDS             complement(69829..71471)
                     /gene="flgK"
                     /locus_tag="OQM47_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71537..72478)
                     /gene="flgJ"
                     /locus_tag="OQM47_000077"
     CDS             complement(71537..72478)
                     /gene="flgJ"
                     /locus_tag="OQM47_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1478381.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72478..73575)
                     /gene="flgI"
                     /locus_tag="OQM47_000078"
     CDS             complement(72478..73575)
                     /gene="flgI"
                     /locus_tag="OQM47_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1478382.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73587..74285)
                     /gene="flgH"
                     /locus_tag="OQM47_000079"
     CDS             complement(73587..74285)
                     /gene="flgH"
                     /locus_tag="OQM47_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1478383.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74338..75120)
                     /gene="flgG"
                     /locus_tag="OQM47_000080"
     CDS             complement(74338..75120)
                     /gene="flgG"
                     /locus_tag="OQM47_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1478384.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75258..76009)
                     /gene="flgF"
                     /locus_tag="OQM47_000081"
                     /pseudo
     CDS             complement(75258..76009)
                     /gene="flgF"
                     /locus_tag="OQM47_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76029..77237)
                     /gene="flgE"
                     /locus_tag="OQM47_000082"
     CDS             complement(76029..77237)
                     /gene="flgE"
                     /locus_tag="OQM47_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1478385.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OQM47_000083"
     CDS             complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OQM47_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1478386.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OQM47_000084"
                     /pseudo
     CDS             complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OQM47_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OQM47_000085"
     CDS             complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OQM47_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1478387.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78947..79606
                     /gene="flgA"
                     /locus_tag="OQM47_000086"
     CDS             78947..79606
                     /gene="flgA"
                     /locus_tag="OQM47_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1478388.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79682..79975
                     /gene="flgM"
                     /locus_tag="OQM47_000087"
     CDS             79682..79975
                     /gene="flgM"
                     /locus_tag="OQM47_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1478389.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79980..80396
                     /gene="flgN"
                     /locus_tag="OQM47_000088"
     CDS             79980..80396
                     /gene="flgN"
                     /locus_tag="OQM47_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1478390.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OQM47_000089"
     CDS             complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OQM47_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1478391.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82171..83004)
                     /locus_tag="OQM47_000090"
     CDS             complement(82171..83004)
                     /locus_tag="OQM47_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1478392.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83006..83653)
                     /locus_tag="OQM47_000091"
     CDS             complement(83006..83653)
                     /locus_tag="OQM47_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1478393.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OQM47_000092"
     CDS             complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OQM47_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1478394.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84484..85692
                     /gene="mdtH"
                     /locus_tag="OQM47_000093"
     CDS             84484..85692
                     /gene="mdtH"
                     /locus_tag="OQM47_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1478395.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85756..86403
                     /gene="grxB"
                     /locus_tag="OQM47_000094"
     CDS             85756..86403
                     /gene="grxB"
                     /locus_tag="OQM47_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1478396.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86537..87097
                     /locus_tag="OQM47_000095"
     CDS             86537..87097
                     /locus_tag="OQM47_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1478397.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87203..88249
                     /gene="pyrC"
                     /locus_tag="OQM47_000096"
     CDS             87203..88249
                     /gene="pyrC"
                     /locus_tag="OQM47_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1478398.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88323..88568
                     /gene="dinI"
                     /locus_tag="OQM47_000097"
     CDS             88323..88568
                     /gene="dinI"
                     /locus_tag="OQM47_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1478399.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88858..89112
                     /gene="bssS"
                     /locus_tag="OQM47_000098"
     CDS             88858..89112
                     /gene="bssS"
                     /locus_tag="OQM47_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1478400.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89227..90345
                     /gene="solA"
                     /locus_tag="OQM47_000099"
     CDS             89227..90345
                     /gene="solA"
                     /locus_tag="OQM47_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1478401.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90393..90506
                     /locus_tag="OQM47_000100"
     CDS             90393..90506
                     /locus_tag="OQM47_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1478402.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90767..91333
                     /locus_tag="OQM47_000101"
     CDS             90767..91333
                     /locus_tag="OQM47_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1478403.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91337..91912
                     /locus_tag="OQM47_000102"
     CDS             91337..91912
                     /locus_tag="OQM47_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1478404.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91954..93006)
                     /locus_tag="OQM47_000103"
     CDS             complement(91954..93006)
                     /locus_tag="OQM47_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1478405.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93231..94151
                     /gene="lpxL"
                     /locus_tag="OQM47_000104"
     CDS             93231..94151
                     /gene="lpxL"
                     /locus_tag="OQM47_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1478406.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94323..94520
                     /locus_tag="OQM47_000105"
     CDS             94323..94520
                     /locus_tag="OQM47_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1478407.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94544..>95068
                     /locus_tag="OQM47_000106"
     CDS             94544..>95068
                     /locus_tag="OQM47_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1478408.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPA"
BASE COUNT        22840 a        25225 c        23472 g        23531 t
ORIGIN      
        1 cacatgtagt gattacatat tcttgatgat cgcatcacca aactctgaac atttcagcag
       61 tttagcgcct tccatcagac gctcgaagtc ataggttacg gtcttggcgt tgatcgcgcc
      121 ttccatacct ttaacaatca ggtctgcggc ttcagtccag cccatatggc gtaacatcat
      181 ctcagcggag agaataatag agccagggtt tactttgtcc tgaccggcgt atttcggcgc
      241 agtaccgtgg gtggcttcaa acagggcgca ttcgtcaccg atgtttgctc caggggcgat
      301 accgataccg ccaacctgcg ctgccagagc gtcagaaatg tagtcaccgt tcaggttcat
      361 acaggcgata acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc
      421 aatcacgtct ttaatgacga tctctttgcc ggtattcggg tttttaactt tcagccacgg
      481 gccgccgtcg atcagttcac cgccaaattc ttcacgcgcc agctggtagc cccagtcttt
      541 aaacgcgcct tcggtgaact tcatgatgtt gcctttgtgc accagggtca cagagtcacg
      601 atcgttagca attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttcaga
      661 acacggctta ataccgatac cgcaatgttc cgggaagcga attttcttca cgcccatctc
      721 ttcacgcagg aatttaatca ctttctcggc gtcggcagag tcagctttcc attcgatacc
      781 cgcataaatg tcttccgagt tttcacggaa gataaccata tcggtcagtt cagggtgttt
      841 aaccgggctt ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag
      901 ttcctgacgc agggcaacgt tgagagaacg aataccgcca ccaaccggag tggtcagcgg
      961 gcctttaatg gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac
     1021 atcctgacca taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt
     1081 acgctcgcct ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt
     1141 tacatctaca ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt
     1201 gagtttgccg ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc
     1261 cattcacctc tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt
     1321 ttgcgtcctg cgatacggat gctttagagc aattttttgt taatgatttg taattgactt
     1381 gtcaatacta ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg
     1441 caattcataa tctctgaaaa taccatgcaa aaaacttctt ttagaaatca ccaggttaag
     1501 cgattcagct cgcaacgttc taccaggcgt aaacatgaaa accagcccac gcgtgtgatc
     1561 ctcttcaata aaccctacga tgttcttccg cagttcaccg atgaaaccgg acgcaaaaca
     1621 ttaaaagaat tcatcccggt tcagggcgtt tatgcagcag gtcgccttga ccgcgatagc
     1681 gaagggttgc tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggt
     1741 aaacgcaccg gaaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt
     1801 gaagccttgc gcaatggcgt aaccttaaat gatggcccta ccctgcccgc aggcgcggag
     1861 ctggttgacg aacccgcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt
     1921 attcccacca gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc
     1981 atgaccgccc atgttggttt ccccacgctg cgactgattc gctatgcgat gggagattac
     2041 tctttggata atcttgccaa tggcgaatgg cgagaagtga cagattaagg atgcaaaatg
     2101 tttaaaccgc acgttaccgt tgcttgcgtg gtgcacgcag aaggcaaatt tttagtcgtt
     2161 gaagagacga ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccgat
     2221 gaaaccttag tggaagccgc cgcccgtgaa ctgtgggaag aaaccggcat cagcgcgcag
     2281 ccgcaacact ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt
     2341 ttcctctttg ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc
     2401 gactgctgcc gttgggtcag cgccgaagaa attttacagg cgtcaaatct tcgttcgccg
     2461 ctggtggcgg aaagtattcg ttgttatcaa agcgggcaac gttatccgct ggagatgatt
     2521 ggcgatttta actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg
     2581 ccgccttgaa gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg
     2641 tcggcatgtc cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat
     2701 atcaggtcga aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca
     2761 cagcagcagc ggatctggct gatgcccagg cggtctgcga caagctcggc attgaactgc
     2821 acaccgttaa ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat
     2881 ataaagccgg tcgcacgccg aatccggata ttctgtgcaa caaggagatc aaatttaaag
     2941 ccttcctcga atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg
     3001 tacgtcgggc cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag
     3061 accagagcta cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg
     3121 taggcgaact ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg
     3181 cgaagaaaaa agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc
     3241 tgggccgtta tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg
     3301 gtgagcacca ggggctgatg tatcacactc tcggtcagcg taaaggtctg ggtatcggtg
     3361 gcaccaaaga aggtaccgaa gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca
     3421 ttctggttgt cgctcagggc catgaacacc cgcggctgat gtctgtcggg ttgattgccc
     3481 agcagctgca ctgggtcgat cgcgaaccat tcaccggcac tatgcgttgc acggtaaaaa
     3541 cccgctatcg ccagaccgac attccgtgca ccgtcaaggc gctggacgac gatcgcatta
     3601 aagtgatttt cgatgagccg gttgccgccg tgacgccggg ccagtctgcc gtcttctata
     3661 acggtgaagt gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat
     3721 tattatcttt acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac
     3781 cctcgccctg gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg
     3841 gcattgtgat gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag
     3901 ctcgacgctg gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct
     3961 gctcggcgtg ctcaatgcca gcagttgcca gggcttaaac gccgaattaa cccgctacac
     4021 actcagcttg atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct
     4081 gggcaaccgg atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac
     4141 gctgatgagc gcgatggctg ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat
     4201 tcaggtcacc ggttcccctg ctgtactgca aagcccacaa gtgcaggcga aagttcgcgc
     4261 aaccctgctg gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct
     4321 acaactgatg ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt
     4381 aaccccggag ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg
     4441 acgctacggc gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa
     4501 attccgtgta caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa
     4561 ggaagttcct gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccaattt
     4621 cagcgaagaa gatgccgcac gcatcaaaac catcgagcgt accactaacc acgacgttaa
     4681 agcggttgag tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc
     4741 tgaattcatc cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat
     4801 gctgaaaacc gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga ttgatggcat
     4861 taaagatctc gccgttcagt accgcgatat cccgctgctg tctcgtaccc acggtcagcc
     4921 agccacgccg tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca
     4981 gcaccgccag cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta
     5041 taacgcccac atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt
     5101 cacctcgctg ggtattcaat ggaacccgta cactacccag attgaaccgc acgactacat
     5161 tgccgaactg tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga
     5221 cgtctggggt tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg
     5281 ttcttccacc atgccgcata aagttaaccc gatcgacttc gaaaactccg aagggaatct
     5341 gggcctttcc aacgcggtat tgcagcatct ggcaagcaaa ctgccggttt cccgctggca
     5401 gcgtgacctg accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct
     5461 gattgcgtat caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct
     5521 gctggatgag ctggatcaca actgggaagt gctggcagaa ccaatccaga cagttatgcg
     5581 tcgctatggc atcgaaaaac cgtacgagaa gctgaaagag cttactcgcg gtaagcgcgt
     5641 tgacgccgaa ggcatgaagc agtttatcga tggtctggcg ttgccagaag aagagaaagc
     5701 ccgcctgaaa gcgatgacgc cggctaacta tattggtcga gctatcacga tggttgatga
     5761 gctgaaataa gcctcaaatc agtgccggat ggcgatgctg tccggcctgc ttattaagat
     5821 tatccacttt ttcttttttc actttacctc ccctccccgc tggtttattt aatgtttacc
     5881 cccataaaca cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat
     5941 gcgcgtactg gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca
     6001 ggatgctggt catcaggtcg atgacgcaga agatgccaaa gaagccgatt attatctcaa
     6061 tgaacattta ccggatattg cgattgtcga tctcggattg cctgacgagg acggtctgtc
     6121 actgattcgc cgctggcgta gcaacgatgt ttcactgccg attctggtat taaccgcccg
     6181 tgaaagctgg caggacaaag tcgaagtatt aagtgccggt gctgatgatt acgtgactaa
     6241 accgtttcat attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg
     6301 tctggcttca caggtcattt cgctcccccc gtttcaggtt gatctctctc gccgtgaatt
     6361 atctattaat gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt
     6421 gatacgcaat aatggcaaag tggtcagcaa agattcgtta atgctacaac tctatccgga
     6481 tgcggagctg cgggaaagcc ataccattga tgtactgatg ggacgtctgc gcaaaaaaat
     6541 tcaggcacaa tatccgcaag aagtcattac caccgttcgc ggccagggct acctgttcga
     6601 attgcgctga tgaaaaaatt actgcgtctt tttttcccgc tctcgctgcg ggtacgtttt
     6661 ctgttggcaa cggcagcggt agtactggtg ctttcgcttg cctacggaat ggtcgcgctg
     6721 atcggttata gcgtcagttt cgataaaact acgtttcggc tgttacgtgg cgagagcaat
     6781 ctgttctata cccttgcgca gtgggaaaac aataagttgc atgtcgagtt acccgaaaat
     6841 atcgacaagc aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg
     6901 gcgcaacgtg acgtgccctg gctgatgaaa atgatccagc ctgactggct gaaatcgaat
     6961 ggttttcatg aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat
     7021 tcgatacagc aacagttgca ggaagtgcgg gaagatgatg acgacgcgga gatgacccac
     7081 tcggtggcgg taaacgtcta cccggcaaca tcgcggatgc caaaattaac cattgtggtg
     7141 gtggatacca ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat
     7201 gtgctctcag ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt
     7261 ttacgcccca tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa
     7321 ttgctcaatc cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg
     7381 ttaaaaagtg aacgcgaacg ttacgacaaa taccgtacga cgctcaccga cctgacccat
     7441 agtctgaaaa cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag
     7501 atgagcgtca gtgatgctga gccggtaatg ctggagcaaa tcagccgcat ttcacagcaa
     7561 attggctact acctgcatcg tgccagtatg cgcggcggga cattgctcag ccgcgagctg
     7621 catccggtcg ccccactgct ggacaatctc acctcagcgc tgaacaaagt gtatcaacgc
     7681 aaaggggtca atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac
     7741 gattttgtcg aggtgatggg caacgtgctg gataatgcct gtaaatattg cctcgagttt
     7801 gtcgaaattt ctgcaaggca aaccgacgag catctctata ttgtggtcga ggatgatggc
     7861 cccggtattc cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact
     7921 ttacgccctg ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag
     7981 ggtaaaatcg tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt
     8041 cgccagcatt ctgcgccgaa agatgaataa atatgtcctt ttaccactac atcaagtcat
     8101 tgattcgatc aaacaatttt cattgaaaaa gtgccaaaaa tcgcaaattg actacaccat
     8161 taactacacc gttcggtgca ctgtatgaaa caacgtggaa caaatagaca caagaaatat
     8221 acaggcgggt aatctttcca gggggaagcg caccaattca tgaggggcgt taatgtcgat
     8281 atggggatcc ccataacggg gctaccggat tttttttccg gttaaccttt aatcaggctg
     8341 gtgggtttta catacccttg atcaccgtaa tgatgatcca gatgtgcacc ttttccacca
     8401 atggcggaaa agctccaacc gtaacgatga tcgctatggt tgcctcgatg gtctgcgtgg
     8461 tcattatgac cgttcagcca gtgaccttaa tcagaatttt tcgctaaaaa atgacgttgg
     8521 ccacgtcatc cggaaagcac aaaatcacgt cgatttttaa tgaacggtta aaccgtcttt
     8581 ttgtcacggt gtgtcactgg ttgtcactct ccgtcacgat tgaggtggtg gtcattctgc
     8641 ccgaccgcgc atctttgcgc tatcgggaat atcaaacagt tacctcgccg acttcctcaa
     8701 attgaggttt ccgaaaatat cagagcgggt tagcgattaa gctgacgagt aattaaactg
     8761 cgaaaatttc gtagtttctg aatatattcc ggcctgctgg ttaacagtcc tcagatgtga
     8821 gggatgtagc cagcgcaatt ttgcgctttg ctttaatctc agtgagttac cgccgtaacc
     8881 gctccggctt cttccagtgg tacgtgattt tctccttctc ccgatacagt tcaacgcggc
     8941 gattgtaggc cagcatttcc agaacgcgga tccgtatgtc gcgcatatcc gcatcattaa
     9001 gctggatacc atcacggcgc atcacctcag caacaacacg cgcataattt tcggctgtca
     9061 cgctgtccgg ctgcgtggtc tgttcgtcag cctgctggct gattccagag acgcggcgga
     9121 ttaatcgcag tatttcggct tctgtcatgc tgctgacctc attacacccc gctaaattct
     9181 tccagtttct ggcggtggct gtcgcttata tcaaaagcaa aatcctcatg ctctgcctgg
     9241 aatgtaccaa acgccatcag cgccgctacg ctcgggtcta tcttgttcgg tgattttttc
     9301 ttgttcggct tgatattggc gttcgcgtca ctctgcatca cgacgttact catcgaccag
     9361 gacaacaccg gatcgccacg atgcacaatc accctgcggt taacaaaaac ttcgaacgat
     9421 ttcgccgccg gactgaatct gaggtaggtt tgcgggaacg gctccacctc aaaaccagcc
     9481 ccctgtaatt gcgtcctgag atgcgtggcg ttccacgtat caaagcccac cagcctgata
     9541 ttaaatttct ccgcatcctg catgatgtca tctctgatcc ggtcgtaatc aatgcagtca
     9601 cctggcgttg tgcgtatcca gcccgcttta gcccactggc gatagacagc gcggttttta
     9661 ttggcggggt tctgtagctg gaactccggc agataatgac gggaaatcag cataatggtt
     9721 ttaccgaccg gaaaggcata gcatacgctg gaaatatcgc tggttgatga taagtccagc
     9781 cccgcgtaac actcctgacc gtgtaaatct tcctccgtga acgttccggc acactcagcc
     9841 catgcaccat tacccatcca cggggtagtc ccctgacacc agatattaaa tcgctttgtc
     9901 atcatctcca cccactgcga cggaataccc cgcgctttct ggatggttga ggccagtttt
     9961 tcacgatcca cggaaacatc aatattaggg ttcgccttta tccacatcgc cggatcatca
    10021 acctcgtttt cgtcgtccag ttcgtaaatc agtacaaaaa ttgaatcgtt aacctcttcg
    10081 ccgtccagga tctggcagca ataatcatag tgctgtttac aggctgaaac gacgttactg
    10141 cccgatgtgg taatggcaaa taacagccct tcgggacgtg cgcccatccc cagctcaagc
    10201 gcggaataaa cgccgttatc ggggtgtagg tggtattcgt ccacgatgga aagactgggg
    10261 tttgtcccct cgatggttgc cgcttttgct gccagcggct ttaacaggct gttgcttttc
    10321 gggtgtatca ccttatgcgc ctgaatattc acccgcctgc gtaacggtcg ggataaaagg
    10381 cacatctgac gcgcattatc aaacacgatc cgcgcctggt cacggctcac cgctgcggtg
    10441 tagatatcct gctgaccgtt ctccataatc agaaaccagt tagccagaat cgcggcggtc
    10501 gtggatttcg catttttgcg cggcacttcg ataaaggcgc tcgtgtattt gcgccgtccg
    10561 gtggccttaa ccttaaagcc gaggatgcac gcaaaggcga actgctgcca cggctccagc
    10621 tcaatgggtc tgccacgcat cggtcctttt acgtgcgggc acaccctgga aaaggcaata
    10681 aaccgctcca caacctcacg atcgaacgtg taaagggggc ttttaaggtc cgaaaagtac
    10741 cgtttaacag cctgttttaa ccgtttacag gccggaattt tgcccgtttt tacgtcttct
    10801 gcgtacttat tccaggcggt caagctcgtc ctcttcttct gtttccggtg gatttttacg
    10861 gcggcttatc gggtcaaaac cgagcaagga ggccattttt atcatcactc tttcagcgtc
    10921 ggattttgcg cttaatgcgg ggtttctgct ctcgccgccc tgactgttaa caatgctgaa
    10981 cccgcgcgcc gcaaggtctg caacggcttt ccggtaaatg gagtagttga cgcaatactg
    11041 ttccagattg ctccagtcgg cgggggtcag gtctccccgt tccgcaagct gccgcgattt
    11101 ttcccgccac tgcttcacgg cgatatcatc caggtaggcg gggtcgggtt tgtcctgaaa
    11161 acgcccccca ccccgtcata cagcctcatc agcgattgcg gaaacattcc ataacctcgc
    11221 ggtcacggtc ggttaatcgc ttcgctgtga tgcgttctgc gcgtcctgac gctttatctt
    11281 tatgccctgt ttcctgtgtc ttccatgcgt cacgctgcct tataagtcca cggataaggc
    11341 ggttttgttc ccgctcattc atcagcgtca tacatccagt tattgcggtt agcagcccgt
    11401 tcttcctctt cacgaaatcc acctgcggca cgcttgcttt ttgttgccgg atcaagccat
    11461 ttcgttttct ggttatgaca cgcctggcac aatggttgat ggttccattc gggccagaag
    11521 agaacatcat caccgccatt aatcgggata atgtgatcca ccaccacggc gggcgtatat
    11581 atccccttct caaggcatcg cacgcataac gggtttttac tcagatacat ggcgcggtat
    11641 ttgtcccact gtctggaata tccacgcgcg cggcggtgtc ctcgtctggc atcctctgca
    11701 cgccatgccg cccgcctgtg ctcctcacac ttgccggatt taacgcgcct gttacagcct
    11761 ggttctgtgc atcgtcttaa tggttgccac ggcatcagta cccccccaca tcacggtaag
    11821 ccgtccagag tgcgcctatc gtcatgggta cgcgtgtttt tgcgttatcc gcgacaatct
    11881 ggcgattttc atacaggtga gcgataaaca tcatgcagcc aatctttatg gctggcgtga
    11941 acgccagccc gtcatcaaag cgcctgccta tgtgtatctg gcacgcctca agcgatgcgg
    12001 caatgtatcc gctgattaac tggtcttcat cgtcgccatc gatgcggcaa tggagtttca
    12061 cttcttccag ggtgataagt tcttctgtca tttttccgcg ccctcacgac aaagaatttc
    12121 aaggcgtgtc ctggcggcat ccggcagcgg ctgcccgatg atattcagta cacgccccgc
    12181 cagcggcccc gtactgacct ttatccggct ggtggcgttg atgtccttcc ggtaccgtat
    12241 ccagatcctt acggtaccaa tcgccagctc tgcgcccgat gaaatggcct ccttgctgct
    12301 gaccatgttc acgcttgccc agagtgtgtg accgtcctcc cacgtttcga gtatttcgcc
    12361 ggttatatcc ctggtctgtt tcagggtctg aatcgttatc ctgtcacgca gtcgccctat
    12421 gttcattccg gtttttctcc ctcgctgatt tttacttcct gtttccatgc ctggctgaac
    12481 tcatcaccac catcacgcgg tgacagccct tcacgttcgc gggcttcgtt cggacacata
    12541 acgtcggatt ttatgccgcg ctcataggtg gcaaagcgtt cgccaggtgt ggcccgtaac
    12601 aggtccgcgc tgtcaaactc cacctgatac cggataccag gtacaggcga ggccaccagc
    12661 aaggcgtttt ttatctgctg ctcaaagttc gccagccacg ggcgcatcgt catggtgaga
    12721 aatgcgcggc ttgcctcgct gaagttgctg taggtgctgt tgctgtattc ctgcaaaaaa
    12781 atcggcgaga cgttgaacat tcgggcgatg tcttcaatgg tgaagcgacg ggaggccagc
    12841 cattcggcat cctgattact catgcccagc tgctgatagc tcatcccccc ttcaaggatg
    12901 ggcgtttttc cggcgtttct ggcccctttg tagcgttcca gtgcggctaa tgcctgtttg
    12961 cccttcacgc cgtccagcca ttcgcctgac gtgataaccc ctgccgccat catgccatca
    13021 cgcatcacgc tcgcgccgtg gcgttgttgg gccagcccaa gccccagcga ttcgcggcag
    13081 atggttacag gtgagcgccc cataaaaccg tcatccgtgg agtaacgaag gtggagaacc
    13141 tcccacggta aatagttgcg gatgtttccg gtgtaggcgt cagtgatgca gtagcgccag
    13201 ttatgatcac ctgtctgctc cacgttcacc gactgcggcg ggtaaggatg taaagccgcc
    13261 ggaaaaccat cacgccccca ctgaataacc gcataagcat taccacttaa caggcagtag
    13321 cggatcatca ttcgcttaaa ctggtagggt gtctgccacg cgttcgggcg ctcgttgagg
    13381 atgtgatcga ccggatgaga atcaagccat tcgcgggcct ccttcccctt ctcattgcgt
    13441 accaggtaca ggtaacacgg catggtagcc accgcgtcag agatgaccgt gacggcgttc
    13501 atgacggcgg ggagtgattc cgctgtccca gatgatacgt actcacccgc ccccgtgttc
    13561 gatgtgccgg ccagcgccat aaattcatca agcgtcatgc tgcgctgctc tttttttcgt
    13621 ctgaaaggcc acatatcaca cccccgctaa atccgcccac cagcggcgat tatccgcacg
    13681 cggcattttt tcggggtgct gctcatacag ggaacggcgg gccagctcca cgccggaatc
    13741 ggggtaagcc ggtaccgatg taacggtaat ttcgtacagt tccgccacca gcacggtgcg
    13801 cacgcatgga tctgttgtgg tatcccatac atccttacgg gagcgaaagc caaagctcat
    13861 ccccgagatg tccccacgct taaccagttc gataacgtcg cgccctgtac tggtatccgg
    13921 tggggtcagt tcaaagcgta accctgtttc gtcctcttcc agtttcagcg tgccggaacg
    13981 ggtgcgcccc agtaacatgc tgtggtcatg ctcatacagg ccgcgaacgt cattacccgc
    14041 cgcaagccat tcagtaaacg ccccccgctg gaatttttcg taaaactcac cccataacag
    14101 ttctgaaagc ttatcccagc gaacaacgta gcccgtcagc gtaccggctc cgctggtggt
    14161 gatttccgat gaccggattt ccatactctt cataattttt tcacccataa aacactaaag
    14221 gggcttttta gccccttctg tatgctgtta atcgtcgtct tccggcagtg ccagaatctt
    14281 gatcgcgttc gaatccacca cgccaccgcc taaatatttt tgcgtgaaaa ttttgatgaa
    14341 tcctggttct gtcaggttgt ccggtctggt gcgaacacct gtttcgtgat caacaatgta
    14401 gtaaccgcgt ttgaagtcac ccagggcaat aacgttatca ggcataaact ccagatattc
    14461 gaccggaagg cccagcaacg tatcaggatc acccgcctgt aaacggtcgc gccagatata
    14521 atcaccgttc gcgttcttca ccttctgaag ttttgccgcc gtcgtggagt taaccactca
    14581 gaccgcgttt ttgcggtatt ttttacgtaa tgcaaatttc aggtcgatca gcgggtccgc
    14641 agatgtccac gccagagatt cggaaggttt aattacctgc aacgtaccaa aatcacgctc
    14701 tttgtcgttc ttctctgcac ggggtacgga taaaaaacct tttgcttttt tgtcaccgtc
    14761 gcccacaacc agatcgcttt cttcggtttc cgtgaaggtg tcgccaatct cacccgtcag
    14821 ccatgaaagg atgtccacat cggaaaaatc cacgatttcc tgtgtggtgc gcgggtacgc
    14881 atagaccgga tacagcttaa tgctcacctc gttaatctgc ggggtgctgg tctgttcgcg
    14941 tgccttaccc tcttcaccgt ggttaacggt cgcaccgcca gcggaaacaa gctgcttaaa
    15001 ctcgttgctg ctgattttct tcacggtaca gatgcgacgc atggtggatt catccgccag
    15061 cattcgcatg atttcggtgt tcagttcagg gataacggta taaccaccat cagcgggaac
    15121 gcctgtactt aatgcgcggg tttcaccggt cagaatgtag ttacgtagtt ctgcggggtc
    15181 agtggtctgg ctgcttttac ctggcttgct gcgctcttcg tctgcaatgg cttcaaggcg
    15241 ggaaatgtct ttatcgaggg attcagcttt agcgcgtaat tcgtcaaatt ttgcgccctc
    15301 agcatcgtta agactgcggt tttctttttc agcgttctcc agcatgtcgc gcatctgatt
    15361 tttaatggcg gttttctgct ggcgtaattc gattattctc tgcataaaaa aagtcctggg
    15421 gttaagtaag gaactccagg acgcggcaaa cactcaaccg tttttcataa ggaaatcagt
    15481 aatcgcaccg atcgttttcc cgcctggtaa tgaatattgg cgagcacatt aacaggcggg
    15541 aaagtggccc cagcgtcctg gcaccacggg cgagaataat catgattcag ttcgggtaaa
    15601 atatgccgat ccgctcagtg aacaacgtgg aacaaccacg aacaaataat ttacaaaaaa
    15661 taacaaaaag ccggattgct ccggctgtat gtgctgtatg ggtgctcagt ctctgatccc
    15721 tttcagaaat tctatcaatg catctgtctg ttcaggattt actgccagca tttcaccgga
    15781 tagcgcgcaa cgaacaaaac catgatgatc cttttcaatc agcgcccccg tttccagaaa
    15841 tgcgcggtaa tcagtgatat tcatcgtctc catgttgtca gcatggtatt tatcacgctg
    15901 tttaagtatc tcatcaaatt tcatcggcat tgcgttttcc tctgttgtgt ctgtttgttt
    15961 cagatagtaa ctatgccgga ccgtgacgaa aagccagtag tgctccatac cgtttcaaat
    16021 gacgcaaaaa aagccggatt gctccggctg cgtggtgtgg ttctgatatt cctactctgc
    16081 taatttgctc agccccatgt ctgcgtaagt gcgatttact gcatttctca ggtctgcgta
    16141 gttctccgtt ggcggctccg gtgtgccctt gctgctctgt tttaccagcg ggtcgcccac
    16201 cagctgcccg taagctgcaa tttgtgctgt catattgcca cctcagtgaa gcgatccggc
    16261 attgttcttt ttcatctctt ccatcatttt ttcagtgagc attacgcact gaaaccccat
    16321 gtttttgatg tctacgggtt tcatgccttt gtcagtggat atcacaggca gaatatcctg
    16381 atacacatcg cggtcagggt aactaagcac acaaagctcc catgatgcca cgggtgagga
    16441 tccttcgtct gtttcaaccc accagcctgg tgtagctgcg cttatttgca gaatcgtgat
    16501 ccgtccgttg tgcttgtaaa atttttcacc aaattttggt tcgctcatga ttccacctct
    16561 ccggttttaa cgttgattgt tgttacctgt tccgcttcgg caatctcccg ctctgtcagc
    16621 gtggcaaaat ttgccgccgt cgtggtcatg aatgcgctta tcagttcggg atgtgctttc
    16681 gcgtatcctt cccccgcgtg gcggtctatc gttctgattg ccacctttaa agcgtgctct
    16741 gtcatgtcta acgcgcgata tttcggttct gttctgtctc tgcgtttttt gaatgattta
    16801 ttaaatttcc ctgaagtgtg catatttatt tttaccccct cgtttaaaaa gttttgagtt
    16861 gtgcctcccc ttgtctacct tatctacctt agtggccctc atgccagtaa tggcgctgct
    16921 ttcagcgggg tagagtgctt ttatccacta tctaccccgt gtctacctcc ctgtctgatt
    16981 caggtaaaat caggtagaga gggtagatag tgggtagaca gtaaaaaaag gctatctacc
    17041 taacttaatg cactgaatta aatgtatttt tctttactca ggtagacagg gtagacagca
    17101 attacaaaaa attataaaaa cgcgtcgcac tcgtctgttg ttattgcgtt agtctgcgtt
    17161 actcccttaa ctttccgcgt aatatattca tgtccgtaaa ctttcgcggc tggcttcata
    17221 gccttgccaa agtcatttac gtttagcggt ttgctcctgc ctgcgtacgc cataaacgcc
    17281 agatagacgc ggtaaaggct gtttctggtc gtgtacttca ctgaatcgcc accgccgccc
    17341 atcatcaggc cgcgtgcttc ctccagaaaa ttcaggaact ggcaaaactc aataaccgga
    17401 tccgtctgtt gctttattgc cagagcttca tcaccgtcac gctgttccag tagtaaagcc
    17461 cgtgctttct cagagtcggt aaagttcgcc agcaatcggc ggataatgac agggatttca
    17521 gccgcaatct tttccggtag ctccctgtct ttttcggcct cactaacgat attgtcgaaa
    17581 cggaaaatca cgcgacgacg tgccacacct ccggcccgtt cggtgaatat catcgggtta
    17641 ttgttggtcg ccagcaccac cgccctgatt acagccgtga aacgcttttc gtatttcggg
    17701 ttaatttcca cggggtcacc gcccgtgatt ttcttgatgc ccgttccttc gcctgtatat
    17761 ttcggctggt cagccaggac gataagacga ctcccgacaa cctgcgcacg tccaccagca
    17821 tcatcaagcg atgtcatttc agcgcttacc gtgttctgtt tccctgccag aaggctggct
    17881 atgtgtgtga atgtactttt accgctcccg ccgtctccgg tggcctcaat aaacatctgc
    17941 cagtcgtacc ggttcgccat aatcatgtac agcgcggcac atatacgcat catcttgcgc
    18001 tggtcttttc cggctgcgtg ctcaagccat ttatgaaagt ttagcgcgtt atcgcggatg
    18061 ttctcccctg gtgctggtgg cgtgtactca atgccgttgt gcgtggtgat ccagttctcc
    18121 ggcgtgtgcg gggaaaattc ccccgttttc aggtcaagcg caccattggc gaacggcagc
    18181 aaatcgccgg acggctcgcc cattggttcg gcaataactt ttaacgcttc cacggcgtta
    18241 ttgattacgc gcttgctgaa agtggccctg tgctctgaat agatcaccac catttcgcgg
    18301 ctaagttcca ttgtgctgac cggacaccat accccgccgc gccatacgtg aacgatttca
    18361 ctttcctgat gcacacaaac gccatcaaag cgctcggcaa gcagctgcgc gcgctcactg
    18421 tctgccattt gtgccagttg cgtatttttc tgctccggca tggtaagccc tgcggcaata
    18481 ttttcacgct cagcattcag atagcgccgc cagttttcac acttctggac gtgcattcct
    18541 tcggggtaaa aatttgcgtc ctgaatatct gccgccgcca gcttctgacc aatctttttg
    18601 gtctccacca aatccagttc tccggcctgg tacagcctta cgcgtttttt tccatccgga
    18661 acaattttca gtgcatcaag ttcggcaagc tgatttggcc caagccacac aggcggcaca
    18721 ttatcgccgg atgcggggcc atcctgctcc tgccattgtt tcgcgtgcga ccaggcatca
    18781 ctacccgcaa agataattac ctctgtgtct ttgtgtttta ttccgcgtgg ctgttttttt
    18841 acgttcggtg ccagtttcat tttttacccc tgaatacgtt aagcatcttt tttatttcct
    18901 gaatattggc gcgtgctttc tccctgctgg ttggtttact gcggggcgct gcctgtacca
    18961 gcgtgaaatc acgccggaat tgataaacag gcatcacgca atcatattcg taaccttcac
    19021 ggcggtaggt gatgcgccgt tctgccacgc ctttaatcgt taccatgccg ccgtactggt
    19081 cgcggtaaat atcaccgcgc gtaaatttag ggtgagtgtt gccactggca gttaagccag
    19141 aatatttaag tttcattatt tttattctcc ggtgtgttgt tctttatatc tgtcgtgcaa
    19201 tagatctatt tcttgcagtt ccattattac aggctcaaga agcgttatta atgccgtggc
    19261 aattcttgat ttttgtttgt cgcgttcatt gtcgccaagt gtttcaagcc atatgcgcaa
    19321 tatttccagc atgttttcac tgtgagaaag tgcaagaaat gcgcggtcta ttgtttcgtg
    19381 gtaaatatca cgcatattaa tccccgtccg tcgtttttct taaaataacc tctgtcacga
    19441 aatcagcata atcggcggcg atattcagta catcaagccc gttgatttat attctctcgt
    19501 ggaaagtaag aaaaaagccg ctctaataag ttctggcatt gacgaaagcg catcagccgc
    19561 atcatcagga acgccggaaa attcctgttt cagggaatta aaacgatcat cacgcataac
    19621 cccccccatt ttcacaatcg gcaacaagaa tatttttagc gtcattcagt gaccgcgttg
    19681 cgatgtatcg gatacattcc agggcgaact gtgtccggtc tttctgttct tctccctgcg
    19741 caagctctgc cgtgcattca atatcaataa gcgcgtgcat cagcgtagtg agtgcggcgg
    19801 ctgctgcgtt cggtgtggtt ttattgtgca taggtttccc cctgacgaat acgggcggcg
    19861 aatacagcaa cacaaccgga cgggcaacgg ctacgcgctt cgcgttccgt ccaagcggtt
    19921 acgtggatga tttgggattc tccggcactc agtgccagaa aacgccacac aaaggccgtt
    19981 tgtgtgtgta caaggtgtgg tatatgattt acggcaacca taacggctcc tagtttacgt
    20041 tgttggttag aagccccgtt actgctccta cagtgcgggg ctttgctttt tgtgttgatt
    20101 gtgtgtaatg tgtcactaca cataaataca ttacctgagg tgtaattaac gtgtcaacac
    20161 acaaaaacga aaggcgtggt aatcctccgt tccagttccg tttagatcct gacttacggg
    20221 aaaagatgga aaaggcacaa caacaggacg gcgatgattc tcttgctgcc tggataaagc
    20281 gaataatccg caaagaatta cagcaacgcg gcatagaacc caaaggataa aaactatcag
    20341 cgccgtggtg tgaggtacta cggcgcattg ctttacaggc gcacacaatg accaacgaag
    20401 aatcaaccga aaaaacatca cctgcacgaa aaagacgacg cagaaagaaa gagcatgaat
    20461 cagaacgatt cgcgccgtgt tcttttgccc ttgagaagta ccttaaagaa tactcatcaa
    20521 aggagagaac ttcgcagtta tggcaacgca ctgaaccaga ctgattgcat tgcccaccag
    20581 cctgatagcg gctatcatcc ccgtgcttat gtttgggatc acacacacaa acggcgcagc
    20641 gggttatctg ttcagaaagg cggctccatt tcggggccgc tttttttatg cctgaaaaac
    20701 cccaattttg tggttttcca gtttcaccag ggcgaacgaa tccccgccca cgttctggcg
    20761 tatattcaat cttcatggtt atagctctgt gttcagatga ttgatgtgtg gcggctgcgt
    20821 gccgccagcg tgattaatga actgccttgc agctatcctt ccaggccaga acctcggata
    20881 aagaccagcc aacggaacga ccgccaagtt tacgacgtga tgggaattgt ccggcctttt
    20941 ccaggcggta gcggcatgag cggctaaggc ctgttagctt ttcgcattct ttttcacgta
    21001 taaaccgatc agtgcttaac actattgccc cctttcgttt cttaaagagt tatttcgtgt
    21061 tctattgcgt tgggatgtgt ctgattgtgt caggatgatt cagagttggc aaatgttgag
    21121 gccgtatggt ttacagaaag aggaataatc aggataaaat cctttaaatt catgttaata
    21181 caaaggcata aaatattgtt tcatgccttt tttctcgcta tttaaagagt gattcgctag
    21241 tgtataaaaa atcagtaaca cattaaaaat cagctactta taaatctgta cactttttca
    21301 cctcttgttc gtagttgttc cgcattgttg ctcattgttg cacgttgtat ctgttcgcat
    21361 atccagtatg cgcatactga aaaaacacga aaaaaattat tttcttctgg ctactggtag
    21421 cgtggttacg ttttcatgtg ttcccgccag tatccctaac cgctccgtcc acatatccag
    21481 cgcattgcgt ttagcatcca gataacggga atgattataa actcgctgca ttcctggcat
    21541 ctggtggcct gtaagctgct ccacgacgtg aggatccacg cctaaatcgt tcagcatcgt
    21601 tgtaaaggtg cgccggatgt catgtagtga ccagtgaggg tgatttagcc tcctgtgcgc
    21661 taatctgccg tactgcgaca cgcttgtttc ctgtttcact tcccccagca ataagcccgt
    21721 gtgcctgttc tgctccacca gctgcgtgac gaacggcagt attgcttccg gtatgggccg
    21781 gaatattgcc accttcgttt tgctgtgttc tttcggcacg gtccagagca tttcggtaaa
    21841 atcccactcg ctgatctccg ataacctcag ttctaccgtc cggcatccga acacaatcag
    21901 gaggcggatt aacgcgatgt agtaggggga gaatattttt ttgtccagtg cctgcaataa
    21961 ttcgcccagt tctttggtgc ttaagacacg ctcgcttata tccggttttt tcccaacgtc
    22021 cgccacactc atatcatcaa gaacgttgct gattgcatag cgccgcctcc ggcagaactt
    22081 aagcgcctgt ttgcacgtct gtagcaagaa tccggcagta acaggcgttc gctttgccac
    22141 ctggtcaaaa caggccagcc agtgccgtag ctcgcattta tccagcggca tagcaccaat
    22201 gtgctgtatt acgtgattat taaggcgctt tttcagggcg gcataatcca cgcggttttc
    22261 ctttgcgtac gactcaagcc agtaggtgag cgcatcgcca accgttaccg gctttaacgc
    22321 ttcctgtacg gtgtaattaa gctcatgacg tggatttttc ccctctgcca gccatgcgcg
    22381 acactgggcg gctttttccc tggctgattt caggctcaga tcaggataat ttcccagctt
    22441 aatgcgttcc ggtggtgccc cccttcccgt tccggccctg taagtgaaat accaggttaa
    22501 aaggccactg gttgaatgcc tgacgctcag gtttccaccg tcattaagaa aggctgtttt
    22561 ttgggcgggt gtgccgttga ttttcctcag ctgtgtatcg ctcagtttgt taagtgctct
    22621 gctcataatt ttgattccgg tcatcacaat taactacacc attaactaca ccgatcgttg
    22681 cacaaagggc tacaacgtga aacaagctgg aacaaggaaa atcacaaatc tgtttataat
    22741 caaaaacata atgaacaatc tgaaacatta tgaaacggca aaaaacacta attgataaaa
    22801 tatgtccata cttcacgcat tacgttaagc atccgttata atcggttgca gataccagcc
    22861 tgtggatgct taacatggaa taccaactca ctcttaactg gcccgatttt cttgaacgtc
    22921 actggcagaa acgcccggtg gtgttaaaac gcggctttaa taattttatt gacccgatct
    22981 ctccagacga gttggcgggt ctggcgatgg aaagcgaagt tgacagtcga ctggtcagtc
    23041 accaggatgg caaatggcag gtcagccacg gcccgttcga aagctacgat catctcggtg
    23101 aaaccaactg gtcattactg gtacaggcag taaaccactg gcatgagccg accgccgcgc
    23161 tgatgcgacc gttccgtgaa ctaccggact ggcgtattga tgatctgatg atttcttttt
    23221 ctgtacctgg cggcggcgtc ggcccgcatc tcgatcagta cgacgtgttt atcattcagg
    23281 gtaccggacg tcgtcgctgg cgagtgggcg aaaagctgca aatgaaacag cactgcccac
    23341 atccggatct gttacaggtc gatccgttcg aagccatcat cgatgaagag ctggagcctg
    23401 gtgatattct ttatattccg ccaggattcc cgcatgaagg ctacgcgctg gaaaatgcga
    23461 tgaactattc cgtgggtttt cgcgcgccaa atacgcggga attaattagc ggatttgccg
    23521 attatgtgct gcaacgtgaa ctgggcggca actactacag cgatccggat gttccacctc
    23581 gtgctcatcc tgcggatgtt ctgccgcaag agatggataa actgcgtgag atgatgctcg
    23641 aattgatcaa ccagccggaa cactttaagc aatggtttgg cgagtttata tcccagtcac
    23701 gtcatgaact ggatatcgcg ccgccggaac cgccttatca gccagatgaa atctacgatg
    23761 cgctgaaaca aggtgatgtg ctggtgcgcc tgggtggtct gcgcgtattg cgcattggcg
    23821 acgacgtgta tgccaatggt gagaagatcg attccccgca ccgtccggca ctggatgcac
    23881 tcgccagcaa cattgcgctg actgcggaga attttggcga tgcgctggaa gatccgtcat
    23941 tcctcgcgat gctcgcggcg ctggtcaata gcgggtattg gttcttcgaa gggtaagttt
    24001 ggatttaagc cggatgcagc atcacatgcc gcatcccttt tcgcttactt ccgttgcgcc
    24061 gttaactcgg caatacggac gatcacctgc accgcttttt ccataccttc cagaatcaca
    24121 aactcatgct taccatgata gttgtaaccg ccagtgaaca ggttcgggca aggtaatccc
    24181 ataaacgaca actgcgcgcc gtcggtaccg ccgcggatcg gtttcagttc cggttcaata
    24241 tcgcagtcac gcatcgcctg ctgggcgata tcgagaatat gcggatgctc aaccactttc
    24301 tcgcgcatat tgtagtaact gtcttcaatc accaattcaa tgtagcaatc aggatgtaac
    24361 cctttgccca cttttttggc gatctccatc attttacgtt tacgcgcttc aaactgttta
    24421 cggtcgaaat cacggatgat gtagtgcata tcggcccgtt caacggtgcc tttcatgctc
    24481 gccaggtgat agaaaccttc atagccttct gtcatttccg ggctttcatc cgccggaact
    24541 tccgcatgaa tacgtgccgc cagcgacagc gcatttacca tcactccttt cgccgtgccc
    24601 ggatgaacat tgttaccgac aatttttata ttgaccgatg cggcgttgaa gttttcaaac
    24661 tccagttcgc ctacgccacc accgtcaaca gtgtaagccc agcgggcatc gaaggcatca
    24721 acatcaaaat gtttcgcccc tttgcccact tcttcatccg gggtaaaggc gacgcgaata
    24781 tcaccatgcg gaatgttttt ctgttgcaat accgccagcg cggtcatgat ttctgcaata
    24841 cctgctttgt catcagcacc taacaaggtt ttaccatcgg tggtaatcaa cgtctgaccc
    24901 agtagctgat gcagcaccgg gaacataacc ggtgataaaa cttcatcgcc gatacccagc
    24961 gcaatatcgc caccgcgata gttttcaaca atttgcggat taacattttt gccgctgcaa
    25021 tccggtgagg tatccacatg agaaataaag ccaatcgccg ggatatcgcc agggacgtta
    25081 gccggtaacg tcgccatcaa agtgcccttc tcacttaagg tcacattgat aagccccatt
    25141 tcttcgagct gctctttcag cagatgcaat aacttccatt ggccttccgt gctgggaacc
    25201 tgtctcaccc ctgcttttga ttgggtatcc agagacacgt agttcaaaaa tcgctcaagt
    25261 agtttatcca tgtagtcacc ctcacttttt gtgacaacat tattaagaag caagaaaaga
    25321 caaatattgc gtcaggtcac ttttacccct gcaagcggga atatttatca gcattacctt
    25381 aatgaatata aagctaagcc agtaattcac aacaaagatt ggcgattcaa gcggtttgcc
    25441 gtagaattac cgccctcatt aggagtcacc aaggtggtta accacaaacc ccgcatcggt
    25501 aagccatccg ttgcgtttac atgggacaga gtaaaaaatt gaataaacaa ccgagttcgc
    25561 tttcaccgct ggtgcaattg gcgggaattc gcaaatgctt tgatggtaaa gaggtcattc
    25621 cccagctgga tctgactatc aacaatggcg agttcctcac gctgcttggc ccttctggct
    25681 gcggtaaaac aaccgttctt cgcctgattg caggtctgga aactgttgat tccggacgca
    25741 tcatgctgga taacgaggac atcacccacg ttccggcgga aaaccgctat gtgaacactg
    25801 ttttccaaag ctatgcactt ttcccccaca tgaccgtgtt cgaaaatgtg gcctttgggt
    25861 tgcgcatgca aaaaaccccc gctgctgaaa ttacgccccg cgtgatggaa gccctgcgga
    25921 tggtgcagct ggaaaccttc gctcaacgta aaccgcatca gctctctggt ggtcaacagc
    25981 aacgcgtcgc cattgctcga gcggtggtta acaagcctcg tctgttgttg ctggatgagt
    26041 cgctctcagc gctggattac aaactgcgta agcaaatgca gaacgagctg aaagcgttac
    26101 agcgtaagct tggcattaca ttcgttttcg tgactcacga ccaggaagaa gcactcacca
    26161 tgtcggacag gattgtggtg atgcgcgatg gtcgcattga gcaagacggc acgccgcgtg
    26221 aaatctacga agagccgaaa aacctgtttg ttgccgggtt cattggcgaa attaatatgt
    26281 ttaacgccac ggtgatcgag cgactcgacg agcagcgcgt acgcgccaac gttgaaggcc
    26341 gcgaatgtaa tatctacgtt aacttcgccg ttgaaccggg gcaaaaactg catgttctgc
    26401 tgcgcccgga agacttacgt gttgaagaga ttaacgacga caaccacgct gaagggctga
    26461 ttggttacgt tcgtgagcgt aactacaaag gcatgacgct ggagtcggtt gttgaactgg
    26521 aaaatggcaa gatggtgatg gtcagcgaat tcttcaatga agacgatcct gactttgacc
    26581 actctctcga ccaaaaaatg gccattaatt gggtagaaag ctgggaggtc gtactggctg
    26641 atgaagaaca caagtaagtt ccagaatgta gtgattgtca ctattgtcgg ttggcttgtg
    26701 ttgtttgtct ttctgcccaa cctgatgatc attggcacca gctttttgac ccgcgacgac
    26761 gccagtttcg tcaaaatggt ctttacactg gataactaca cgcgtctgct cgatccgctc
    26821 tattttgaag tgctattgca ctcgctgaat atggcgctga tcgccaccct cgcctgcctg
    26881 gtgctgggct atccatttgc ctggtttctg gcgaagctgc cacacaaggt gcgtccgctg
    26941 ctgctgtttc tgctgattgt tccgttctgg accaactcat tgattcgtat ctacgggctg
    27001 aaaattttcc tcagcaccaa aggctatctc aacgagtttt tgctctggct gggcgttatc
    27061 gacacaccaa tccgtatcat gttcacgccc agtgcggtga ttatcggtct ggtttacatt
    27121 ctgctgccgt ttatggtgat gccgctgtac tccagtatcg aaaaactgga taagccgttg
    27181 cttgaggcgg cgcgcgatct cggtgccagc aagttacaga cttttatccg catcattatt
    27241 ccactgacga tgccggggat tattgccgga tgtctgctgg tgatgctgcc agcgatgggc
    27301 ctgttctatg tgtccgacct gatgggcggt gcgaaaaacc tgctgatcgg taacgtcatc
    27361 aaggtacagt tcctcaatat tcgtgactgg ccgtttggtg cagccaccag cattacgctg
    27421 actatcgtaa tgggcctgat gttgctggtt tactggcgcg cttctcgttt gctgaataag
    27481 aaggtggaac tcgaatgatc ggtcgactgc ttcgcggcgg ttttatgacc gctatctacg
    27541 cgtacctgta tatcccaatc attattttga ttgtgaactc ctttaacagc tcgcgctttg
    27601 gcatcaactg gcagggtttt accaccaaat ggtatagcct gctgatgaac aacgacagcc
    27661 tgttacaggc agcgcagcat tcactaacaa tggcggtgtt ttcggcgacg tttgctacgc
    27721 ttatcggttc actgacggca gttgcgctgt accgttatcg ctttcgcggt aagccgttcg
    27781 ttagcggaat gctgtttgtg gtaatgatgt caccagatat cgtgatggca atttcgctgc
    27841 tggtgctgtt tatgctgctg ggtattcagc ttggcttctg gtcgctgctg ttctcgcata
    27901 tcaccttctg cctgccattt gtggtggtga cggtgtattc gcgcctgaaa ggttttgacg
    27961 tgcggatgct ggaagcggcg aaagatctcg gtgccagcga atttaccatt ctgcgaaaaa
    28021 tcattctgcc actggcaatg ccagcggtgg cggcgggctg ggtgttaagc tttaccctgt
    28081 cgatggacga tgtggtagtt tcttcattcg tcaccggacc aagttatgaa attctgccgt
    28141 taaaaattta ttcgatggtc aaagtcggcg tatcgccgga agttaacgcg ctggcaacca
    28201 tattactggt gctgtcgctg gtgatggtta ttgccagcca gcttattgct cgtgataaaa
    28261 cgaaaggtaa cacaggggac gttaaatgaa aaaatggtca cgccacctgc tcgcggcggg
    28321 tgctctggca ctgggcatga gcgccgctca cgccgatgac aacaacacgc tgtatttcta
    28381 caactggacc gagtacgtgc cgccaggact gcttgaacag ttcaccaaag aaaccggtat
    28441 taaggttatc tattcgactt acgagtcgaa cgaaaccatg tacgccaagc tgaaaacgta
    28501 caaagacggt gcctatgacc tggtggttcc ttcaacctat tacgtcgata aaatgcgtaa
    28561 agaggggatg atccagaaga tcgacaagtc gaagttaacg aacttcagca atctcgatcc
    28621 agacatgctc aacaagcctt ttgacccgaa taacgactac tccattccgt atatctgggg
    28681 tgcgacggcg attggtgtta acggtgatgc ggtggatccg aaatctgtca ccagctgggc
    28741 cgatctgtgg aagccagagt acaaaggcag cctgctgttg accgacgatg cccgtgaagt
    28801 gttccagatg gcgctgcgta agctgggcta ctccggtaac accaccgatc cgaaagagat
    28861 tgaagctgca tataacgagc tgaaaaaact gatgccaaac gtcgcagcgt ttaactccga
    28921 taacccggct aacccgtaca tggaaggcga agttaacctc ggcatgatct ggaacggttc
    28981 tgctttcgtt gcacgccagg cgggtacgcc tattgacgtg gtgtggccga aagaaggcgg
    29041 catattctgg atggacagcc tggcgatccc ggcaaatgcc aaaaacaaag aaggcgcgct
    29101 gaaattgatc aacttcctgc tgcgcccgga tgtggcaaaa caggttgctg aaactatcgg
    29161 ttatccaacg ccaaaccttg cggcgcgtaa gctgttaagt ccagaagtgg caaacgataa
    29221 gacgctctac ccggatgctg aaaccattaa aaatggcgaa tggcagaatg acgttggcgc
    29281 agccagcagc atttatgaag agtattatca gaagctgaaa gcaggacgtt aattcagcaa
    29341 gcgggcggtg gtattaccgc ccgtttttta gttagcaagg atgtggctaa tgagtcagga
    29401 cagtaaagtt ttatcccgct ctttcgtcgg tattgtggtc cgcttgtagg cctgataagc
    29461 gtagcgcatc aggcaatgct tcctgcttta taatccctcc agcaactttt caacaaattc
    29521 tggcaccacc tggcttgccg ggccgtaata tttctcggca aattcattac caacctgact
    29581 cggttcaaga ttcagttcaa cggtgtgagc accatgtagt ttggcttcgt gtacaaaccc
    29641 agccgccgga tagacatgcc cggaagtacc aatggcaata aagacatccg ccatcgacaa
    29701 cgccatataa atttcatcca tgccgagtgg catttcgcca aaccaaacga cgtgcgggcg
    29761 caagggtgcc gggaactggc aacaatggca tttatcttct ggcgtaacgt ctccggtcca
    29821 gtcgagaacc tgaccacttt gcgaacaacg aactttcagc aattccccat gcatatgaat
    29881 gacattggta ttacctgcgc gttcatgcag gttgtctata ttctgcgtta ccagcaaaaa
    29941 gcgatcgccg agagcgtctt gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat
    30001 ttctggctgc tgcagctgtc gacgacgggc gttataaaac gcttgcacca gttcaggatc
    30061 gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc acaggccatc
    30121 tgcggcgcga aaggtacgaa tacctgattc cgcagaaatt cctgcccctg tcagtacgag
    30181 tactcttggt ttttccattg cttccggcac cactttatct ctgaaaaaaa tacgctgacg
    30241 caaacgctcg cgcaggcgac gtttattttt acgaaaacga cttaaccgat gaccccgacg
    30301 cgacagcata gcaacctctg ttgtttaatc ggttagatgt aggaaggccg caccacgcat
    30361 tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga acacgagcta caggtaagag
    30421 atgacaaggc aacctgtccg ccagttgcgt tgtgattgcc gggaaattcg ataagccacc
    30481 accaatgacg accaggtcag ggtcaacaat ggtcaggata tttcccagag aaaccgctaa
    30541 taaatccaga taacgctcaa cgtgcgccct tgcctgctca tcgccttgat cataaagcgc
    30601 aataatttcg ggagcctgca acggttgatg ataatagtgt tgatacagcc acgcaaaacc
    30661 gcgaccagac agataatttt caatgcagcc atgctgacca cagccgcagc ggcgtaacgg
    30721 gaaatccaac cccatcatgg ttaacgcatc aaccggcaga cgcatatggc caaactcgcc
    30781 ggtaatgtag cttttaccag taatcggttt gccgttgaaa accagcccgc cgccaacgcc
    30841 ggtgccgaga attaacccca tcaccagtgg atattgcgta aattcatcat cccaggcttc
    30901 tgaaagggca aaacagttgg catcgttatc aaggcgtaca tcgcgatcaa gacgtgcgct
    30961 caggtcggca cgcagcggtt tgccgctggc agcggggaca ttagcggcat acagcttacc
    31021 atcttctgtt tccggcattc ccggaatacc gatgccgaca gaacctttac agccaaaacg
    31081 ttggtcagct tcagctacca gctcacacac tgcatctaaa aatgcgtcat agctgtcacg
    31141 cggtgtcggc acccgctttt cccactgcaa ctgccgaccg ctatcaaaca cgccaagcgc
    31201 aatttttgtt ccaccaatat caaacccgta atacattgcc gctccttgtt ttaatgtact
    31261 gcctttactg gccgctaagg actcgcgcag ggtcaatatt actggcgcgc cgcgccggat
    31321 accaacttgc caaaagactc agcaacaatg ctgtgaccag tacgtagaag acgtccagcc
    31381 agtgcaattc cgatggcagg aagtcaataa aataaatatc gctggagagg aactgatgac
    31441 cgatcagctt ttcaatccac tcaataatcg gggtaagttg cagtgaaaca actacgccga
    31501 taaccacccc acacaggctg ccgaacagcc ctgccagcaa tccataccag acaaagatgg
    31561 cgcgaattaa accatctttc gcccccagcg ttcttaatac tgcgatatcg ccactcttgt
    31621 ctttcaccgc catcactaag gtggagacga tgttgaaaca ggccacgcca atcaccagta
    31681 ccatcgccag atacataatg gcgcggatca tctggatatc gcgatacata tagccgtaag
    31741 taccaatcca acttttaata taaacatagc tgttggtcac ttcacccgca tcgcgtacca
    31801 gcttattggc gttgaaaaca tccgtcattt taagggcaat acctgacacg ctggaaccca
    31861 tatcaagata ttgttgggca tccgccagcg ggatcatggc aaaactgtga tcgagttgac
    31921 cactcaactg caaaataccg gcaatgtgca aacgcacacg ttttggctgc atcagtttat
    31981 gctcgggatt cgagttgggg atcatgatcg acacccaatc gccctgcttc actttcagcg
    32041 catccgccac gcctttgccg atgataattt gctgttcgcc cgctttaaaa ttgcgccacg
    32101 catccccctg aacaaacgag ggtaatgcgc tcagacgctg ttcctgttgt gggttaacgc
    32161 ccttcacctg gattgcgcgc agattcgctc cactttccac cagaccggtg aaattgatat
    32221 acggcgcagc ggcggcaata cccggcactt tctgcacgtt atccagtgct tcctgccagt
    32281 tagtccatgg ctgatttacc gcttcgattt cgccatgcgg caccaccgcc agaatgcggt
    32341 tattcagttc gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat accgccacgc
    32401 cgagggcaat gccaatggta gaaatgacag agattagcga caccatgccg ccgcgtcgcc
    32461 gtccgcgact aaaacgcagg ccaatcaata acgataaagg catcgccatt actccgcccc
    32521 catcaggctc agttccgccg tcagacgtcc atcacgcatt tccagttggc ggctcatacg
    32581 tttcgccagt tgcaggtcgt gagtgaccac caggaaagcg gtgccctgca agcgattcaa
    32641 ctccccaagc aactggaaga tgctgtcggc gttacgcgca tcgaggttac cggtaggttc
    32701 atccgccagt accagacgcg gattattgac cagcgcacgg gcaatcgcta cacgctggcg
    32761 ttcgccgcca gaaagttcag atgggcggtg attcgcacga tgctccagcc ccaccgcttt
    32821 taacatctcc agtgcacagc tgttgatttc agcgggcttt ttcttgccaa tcagcagtgg
    32881 catagccacg ttttccaggg cagtaaaatc cggcagcagg tggtgaaact gataaataaa
    32941 gcccagcttc tggttgcgca gttctgcttt cgccgctgaa gacagtttgc tcatcggctg
    33001 accgttaaag atcacatcgc cggaggttgg cgtatccagc ccgcctaaca ggtgcagcaa
    33061 ggtactttta ccggaaccag agctaccgac aattgccatc atttcacctt cgcccacgct
    33121 gaaactgaca ttgagcagca catcggtttg cacactgcct tcctgatagc gtttgcacag
    33181 gttgtcgcat tgcaacagga tcttattcat aacgtaaagc ctcagcgggt tgtgtggcgg
    33241 cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat agccatcgcc accagcgcaa
    33301 taacaatgac ctgtaaaggt tcgatagcca ccggcagcgc cgcgccatca agcaggacgc
    33361 cgattatcgg catcagatta tttaactggc tggcaagcag tgcgccaagc gccgctccga
    33421 ggattgcacc gataatcccg gcgctagccc cttgcaccat aaagaccatc atgatttgtc
    33481 gcggagttaa gccttgcgtt tgcaggatcg cgacttcgcc ctgcttctcc atcaccatca
    33541 gccccagcga ggtaataatg ttaaacgccg caacagcgac aatcaggctc agcagtaagc
    33601 ccatcatatt tttttccatg cgtacggcct ggaacagctc gcctttacgg tcacgccagt
    33661 cctgccattt gctgccttca ggcagttttt gctgacttaa agagtcaact ctcagcggct
    33721 catccagcca caaacgccag ccggtaatat tggctgccgg ataacgcatc agacgcgagg
    33781 catcctcaat attcaccagc atttcatagc catcgacttc actgttggcg gcgaaagtgc
    33841 caatcacatt gaacaggcgc tgacttggaa tacgccccat cggcgtgaac tggctggcag
    33901 atggcaccat cacgcggatt tgatcaccgc gattgacgcc caactgtgag gcaagttgtt
    33961 cgccgaggat gacattatat ttccccggct cgaggtcagt ttgtttcaca ttgaccagat
    34021 acggcgttag tggatctttt tgcgccgggt cgataccgag catcaccccg accgccacgc
    34081 tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt
    34141 ccagtttgac tgccgtttcc gggagttgct gcgggttaag agagccatgc tcagaagaga
    34201 gaattgcctg tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca aagccgttca
    34261 tcactgacaa tactgtgacc agcgccatca ccccgagggt aatgccgatg gtagaaagcc
    34321 aggagacgaa acgaccgaag cgatccactg cacgcccacg catgtaacgc aggccaataa
    34381 atagagcgac aggttggtac atgaaatccg tctggttgct gtagcaaaga cacgagtata
    34441 taagcgaaag caagatgggt aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa
    34501 aagcccccgt aaatcaatcg gctaaattgc catattttat catcaatctt gccgcctgat
    34561 cttaatctga aaaatcatcc aatacagaca gatacccatt acacctcggg tcataaccag
    34621 caggatcaac cgcatgtaaa ttaaaaaaga gcgtgttgtt ctgatgaaac aaaaagagct
    34681 atggattaac cagatcaaag ggttatgtat ttgtctggtg gtgatttatc actcggtcat
    34741 taccttttat ccgcatatga ccacttttca gcatccgtta tcggaagtcc tgagcaaatg
    34801 ctggatctat ttcaatcttt accttgcccc ctttcgtatg ccggtttttt tctttatatc
    34861 cggctatttg attcgccgct atatcgacag cgtaccgtgg ggaaattgtc tcgataaacg
    34921 gatctggaac atcttctggg tgctggcgct ttggggcgtg gtgcagtggc tggcgttaag
    34981 tgcgctgaac cagtggctgg cacctgagcg cgatttaagc aatgcctcca atgccgctta
    35041 tgccgattcc accggtgagt tcctgcacgg gatgatcacc gccagcacca gcttgtggta
    35101 tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt tttagccgtc tggcgctgcc
    35161 actattcgcc ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg
    35221 gggaatgaac agtgtgatac gcaatttgcc ttattacagc cttggcgcat ggtttggcgc
    35281 aacaataatg acctgtgtta aagaggtgcc gttgcgccgc catctgctga tggcttcttt
    35341 gcttaccgtt ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt
    35401 atcgattgtg gtgatcatga agctgtttta tcagtacgag caacgattcg gtatgcgctc
    35461 caccagcctg ctgaatgtga ttggctccaa caccattgct atctacacca cccatcgcat
    35521 tctggttgaa atattcagct taactctgct tgcgcaaatg aacgcagcac gctggtcgcc
    35581 gcaagtcgaa ctgacactcc tgctggttta tccctttgtc agtttgttca tctgtactgt
    35641 tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc agcgatctgt tgttctcccc
    35701 gccttcactg cccgctgccg tcagttactc tcgttaaacc atcaccccag ccggggcgat
    35761 tcaatttgct aaaccatgtc gattacggat aatgaacagc attgcttatc agagatatgc
    35821 ccccatatgt tgaggcatat cctaacgaga atctgacaac cgttatgcct gaacaatatc
    35881 gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta accggcgcag
    35941 cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc cggtccggtg gtactcattg
    36001 caccagatat gcaaaatgct ctgcgtttgc atgatgaaat cagccagttc accgatcaga
    36061 tggtgatgaa tctggcggac tgggaaactc ttccctacga cagtttttcg cctcatcagg
    36121 acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag cgtggcatac
    36181 tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc acacagtttt ctccacggtc
    36241 atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga acccaactgg
    36301 acagcgccgg ttatcgccat gttgaccagg tgatggagca cggcgaatac gccacgcgcg
    36361 gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt gatttctttg
    36421 atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag
    36481 tagaagcgat caatctgctg cccgcgcacg aatttccgac cgataaagcg gcaattgaac
    36541 tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccggaa catatttacc
    36601 agcaagtgag taaaggcaca ttacctgctg ggatcgagta ctggcagcca ttgttcttca
    36661 gcgaaccact gccaccgctg ttcagttatt tccctgccaa taccttgctc gtgaatactg
    36721 gcgatctgga aaccagtgcc gaacgtttcc aggctgacac gctggcgcgt tttgagaatc
    36781 gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc gctctggctg cgggtggacg
    36841 agctcttctc agagctgaaa aactggcccc gagtgcagct aaaaactgaa catttaccga
    36901 caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc gttcaggcgc
    36961 aacaaaaagc gccgctggat gcgctgcgta agttcctcga gactttcgac ggtccggtgg
    37021 tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta
    37081 aaattgctcc gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg cgttatctga
    37141 tgattggcgc tgccgaacat ggttttgtcg ataaggcgcg taatctggcg ctgatctgcg
    37201 aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca ggattctcgc cgcaccatca
    37261 accccgatac actgatccgt aaccttgcgg agctgcatat tggtcagccg gtggtccatc
    37321 tggagcacgg cgtcggtcgc tatgccggaa tgaccacgct ggaagctggc ggcattactg
    37381 gcgagtacct gatgctcacc tatgccaacg acgccaaact gtatgttccg gtgtcgtcac
    37441 tgcatctgat tagccgttac gcaggtggcg cggaagaaaa cgccccgctg cataaacttg
    37501 gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg
    37561 cggaattgct ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg
    37621 atcgtgagca gtatcagtta ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg
    37681 cgcaggccat taatgcggta cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg
    37741 tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat gcgcgccgct ttcctggcag
    37801 tagataacca caagcaggta gcggtgttgg tgcctaccac ccttcttgcg cagcagcatt
    37861 acgacaactt ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg atctcccgtt
    37921 tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata
    37981 ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc
    38041 tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc
    38101 gcgcgaacgt ggatattctg acgcttactg caacgccgat cccacgtacg ctgaatatgg
    38161 caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag
    38221 ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg ggaggcgatc ctgcgtgaaa
    38281 ttttgcgcgg aggacaggtt tattatctct acaatgatgt ggaaaacatt cagaaagccg
    38341 ccgaacggct ggcagaactg gtgcctgaag cacggattgc catcggtcac gggcagatgc
    38401 gcgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc aacgtgctgg
    38461 tttgtaccac cattatcgaa accgggatcg acatcccgac agccaacacc attatcattg
    38521 aacgtgcgga tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt
    38581 cacatcatca ggcatatgca tggctgctga cgccgcatcc aaaagcgatg actaccgatg
    38641 cacaaaaacg tcttgaagcg attgcctcgc tggaagatct cggggcaggt tttgcgctgg
    38701 caacgcacga tctggagatt cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct
    38761 caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac
    38821 tgaaagccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca gaagtcgagc
    38881 tgcggatgcc gtcgctattg ccagatgatt tcatccctga cgtgaacacg cgtctgtcgt
    38941 tctacaaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc
    39001 ttatcgatcg cttcggcctg ctgccagatc cggcgcgtac cctgctggat attgcccgtc
    39061 tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag aaaggcgggg
    39121 tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt ttgctgcaaa
    39181 aacagccgca gcattaccgc cttgatggtc cgacgcgcct gaaatttatt caggatttga
    39241 gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg gaagagaacg
    39301 cgatcgctta atgtaaatcg ggggcgtttc tgcgccccca tctgcaacat ttacaaattc
    39361 tttgcacttt cctgcactat ccgacactgt caccatccat aattcaggct tatctgttta
    39421 ttacaataac cttatattta ttatgtattt ttggtgatga tcaaaacgcg tttttctcgc
    39481 tggctaacgt tttttacgtt cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac
    39541 acctggccgc tgccgccagc gagcagtcgt ctggttggcg aaaacaaatt tcatgtggtg
    39601 gaaaatgacg gtggttctct ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct
    39661 ctgttacaag ctaaccccgg cgttgatcct tacgtaccgc gcgcgggcag cgtgttaacg
    39721 atcccgttgc aaaccctact tccagacgcg ccgcgcgaag gcattgtgat caacattgcg
    39781 gagctgcgtc tctattacta cccgccgggt aaaaattcgg taaccgtgta tccaataggt
    39841 attggtcagt taggtggtga cacgctgaca ccgacaatgg tgaccaccgt ttcagacaaa
    39901 cgtgcaaacc caacctggac gccaacggca aacatccgcg cccgttataa agcacaggga
    39961 attgagttgc ctgcggtagt gccggctgga ccggataacc caatgggcca tcatgcgatt
    40021 cgtctggcgg cctatggcgg cgtttatttg cttcacggta cgaacgccga tttcggcatt
    40081 ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg acgatatcaa aacactcttt
    40141 agccaggtca ccccaggcac caaagtgaat atcatcaaca ctccgataaa agtctctgcc
    40201 gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc tgtcagagaa gattgatgac
    40261 gatccgcagc tgctgccaat tacgctgaat agcgcaatgc aatcatttaa agatgcagca
    40321 caaactgacg ctgaagtgat gcaacatgtg atggatgtcc gttccgggat gccggtggat
    40381 gtccgccgtc atcaagtgag cccacaaact ctgtaaatcc tggataaaaa aaatccccgc
    40441 gacacggcat gtcgcgggga aaaaatcagc agtagcaggc attaatgagg gttaatgctt
    40501 atttataaat tactgctgtt ccatggaggg tattcggacc ggttacagaa gtaatacgga
    40561 aagattttgc gcccatctca tccgcttttt gcgccagctg ctcttccagc gatcccagat
    40621 ttgtccccgc gttagcactg atagtaccga ctttttgttg gccttctggc gttgactgaa
    40681 cttcgacagc cgcaaagttg gcaaatgaca tggagcttaa aatcgccgca gcgatgaggt
    40741 ttttacgttt ttcataatag tggccttatg cagatgaatg acgtcgcaag aaattactta
    40801 acgatcgata actaaatgat agatgtgatc tggatcacat acaagatatt ttttataaca
    40861 atcattaatt aatttaaaaa tctttaaatt tcagtcaaat agaaagcaat tatttttatg
    40921 ggatctggac tggtgaacgg aggtgattat ttttataata acctttcatt cacagtcacc
    40981 aggtacaacg gcatcatggc aactgactca acacaatgtg taaaaaaaag ccgtggccgc
    41041 ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca tgaaattgtt ctggcaacac
    41101 ggttatgaag cgacttctct tgcggacctc gtcgaagcaa ccggagccaa agcgcccacg
    41161 ctatacgcgg aatttaccaa caaagagggg ttatttcgtg ccgttctcga ccgctatatc
    41221 gatcgttttg ccgctaagca tgaagcacag ctgttttgtg aagagaaaag cgtggagtct
    41281 gcgctggctg actattttgc tgccatcgcc aactgcttta ccagcaaaga caccccggca
    41341 ggctgcttca tgatcaacaa ctgcaccacc ctctccccag attcaggaga tatagccaat
    41401 acgttgaaat cacgccatgc gatgcaagag cgcactttgc agcagttttt atgtcaacga
    41461 caagcgcgcg gggaaatccc gacccactgt gacgtgacac atctggcaga attccttaat
    41521 tgtattattc aggggatgtc gatcagcgca cgcgaaggtg catcgctgga aaaactgatg
    41581 cagattgccg gaacgacttt gcgtttatgg cccgaactgg tgaaataagt cgcaaaaaag
    41641 gcccctcagc ctgagcgaat gaggggccaa attgcagagt acagccttgt tatactttgt
    41701 tattcaaaat cagttgcccg ttactatcta gcgggatctg cgtacccgga tcgcggtcca
    41761 tgcggatttt gccctgctga tcgccaatct tataggtcac atcataaccg agcatttttt
    41821 ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt cgtgtaagta tcgctttcct
    41881 ggagagagcc ctggatctgg ttaccggcat atccaccacc cagcgccccc acaacagtgg
    41941 cgacatcttt accgcgacca ccaccaaact gatgccctat cacgccacca gcaacagcgc
    42001 cgagcaccga cccggtaatg cgattttcat cctgcaccgg tcgacgatgg gtcactgtga
    42061 cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt gattggggtt gcagaaacaa
    42121 cctgagcgta ttgcgggccc cgttcaaaca cgttcagact ggccactgcc gctacgccca
    42181 acgcagctgc gacaccaatc ccgatacccg ccaacattga tttattcacg ggaacacctc
    42241 cttctttgca gttatgccaa tcatgcaagc gaaggagaaa agcagctatc agacagaaga
    42301 tgaaaagcgc gggaatgact ggaaatactt aaggattcgg aggagtctga aggaatgcct
    42361 gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt accatttgtc cagggcggat
    42421 aaggcgttta taccacatcc gccagtgtac gtcgattaat gcaacttcaa acgcggacgg
    42481 ataacgcggt taatactccc caccagcatc attaatccgg ttttaaagta accatgcagc
    42541 gcaatctgat gcattcggta tagcgagata tatacaaagc gcgcaattcg tccttcaatc
    42601 atcattgagc cgcgcgtcag gttacccatc aggctaccaa cggtggagaa gttcgacagc
    42661 gataccagcg aaccatgatc tttatactga taatttttca gcggcttacc gttcatctgc
    42721 gccagaatgt tgttcattgc gcaagtcgcc atctggtgtg cagcctgagc acgcggcgga
    42781 acaaagcccc cttccggacg cgggcatgac gcgcagtcgc caatagcgta aatgtctgga
    42841 tcgcgagtgg tttgcagcgt cggttccacc accagctggt tgatacggtt agtttcaaga
    42901 ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg ctgcccatac catcagatca
    42961 gcctcaatat attcgccatc tttagtgtgc aggccgcctt catcagcact ggtgaccatg
    43021 gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt tgtgggccgc agcagagata
    43081 cgtggcggta atgcaggcaa aatacgttct cccgcttcta ccagcgttac gttcagggct
    43141 tcgttggtca ggcctttgta accgtagctg tgcagttgct tgatcgcgtt gtgcaattca
    43201 gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg caatgttcac tttgccattt
    43261 gcgcccaggt tggcagagta tttcaggaac agattcagca tctcctggtg gaagcgacgc
    43321 gcctggtgcg ggttatcgag gaaaatgcag ttctctttga cgcctggcgt attgaaatcg
    43381 ttagaggtgc tacccagcgc catgaccagg gtgtcatagg cgattttacg ttccggaacc
    43441 aacagttcac ctttctcgtc gcgtagttct gcaatagtga ttgttttcgc ttcacgatca
    43501 atatcaatga cggaacccaa ctggaactgg aaaccatgat tgcgcgcatg ggccagatag
    43561 ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca cttcgtgcag cagtggtttc
    43621 cacaggtggc tgtggttacg atcgaccagc gtaattttgg cttttttctt acgtcccagc
    43681 ttatgcccca gctgtgttgc catttccagc ccaccagcac cgccgccgac aatcacaatc
    43741 tttttcaatg gcgtagtcaa cgtgcccccc ttaaatttat taaccaattg ttaattaatg
    43801 gttattaaca tagctcttaa ttaacaacag gttacagcaa tgaaaatgtt cttcgctatt
    43861 gagaataaca tgaatggtgc attggtcata ccaaaattga tgttaatcaa gttttgttgc
    43921 ttaaaagata ggcaacttta ccgggggaga tcaaaaaata gagtttattg tttattattt
    43981 gagcaaattc ggaagggtaa tatagggtga agcgtatatc gagggcgttt ggtcgtcctg
    44041 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc
    44101 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    44161 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    44221 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
    44281 atgtccgctg gctgttaagt gcttcatcat tacgcgacaa aataaccaga caacgatcgc
    44341 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    44401 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    44461 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    44521 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    44581 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    44641 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    44701 agataatcat cgcttcctca cataagccag ggttagtgac ctactttctc ttcctgccag
    44761 cgttcatgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    44821 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    44881 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    44941 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    45001 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaacc ccagttcctg acgcaaaacg
    45061 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    45121 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    45181 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    45241 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    45301 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    45361 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    45421 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    45481 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
    45541 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    45601 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    45661 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
    45721 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
    45781 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    45841 ccagcggtac tcaaaccacc ctcctttgag catcagcagc cagggaaacc atcgcctgac
    45901 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    45961 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    46021 tccagcatac tcccagtcga tgagttttaa ccctgatgcg ctatgcacta aatttccggc
    46081 gtggacatcc atatgcaatg gacttaagcg taaaggtcgt ggttcccgcg ctttgcgcag
    46141 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
    46201 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    46261 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg caccgggcag
    46321 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    46381 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    46441 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
    46501 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    46561 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    46621 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgcagg
    46681 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc
    46741 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagt tgctgggcgg
    46801 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    46861 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    46921 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    46981 cataatggcg aatatgcggc gcaggcggtg ccgttcgatc ttcgtgctca attgggcctg
    47041 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    47101 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
    47161 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    47221 ttcacccctt tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    47281 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    47341 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    47401 ttgcctttcg ttataaatcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    47461 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    47521 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
    47581 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    47641 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    47701 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
    47761 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    47821 gtcggaatga gaatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    47881 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    47941 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    48001 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    48061 ttcaatctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    48121 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
    48181 aaatatgaat gcgtatattt ctcatttgca tttgcatttg cagcaaaatt attcgcacac
    48241 taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag
    48301 atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagtca tcaccaaacc
    48361 gtcactactt gccggttgca tagcactggc actattacct tctgccgctt ttgctgcacc
    48421 agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa
    48481 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacatgatat
    48541 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
    48601 gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc
    48661 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
    48721 ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactgt ttgaacgcgt
    48781 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
    48841 taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa ggcgatgtct cggcggaata
    48901 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
    48961 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
    49021 caatagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg atcttacgac
    49081 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agcccaacct ggggcggttt
    49141 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc
    49201 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
    49261 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
    49321 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
    49381 taattatgga cctggctttg atcatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
    49441 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
    49501 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tcagttcatg ggccaacatc
    49561 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca agccgactgg
    49621 tcatcacaga gcctggcgca ggacgatacc acacatatga aatcgttata tgctgccact
    49681 cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg
    49741 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
    49801 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
    49861 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
    49921 ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
    49981 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
    50041 gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca
    50101 attaccgaca actggcagct gacatttggc gcaacgcgct atcttgcaga ggataacgaa
    50161 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg
    50221 ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat
    50281 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
    50341 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac
    50401 ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt
    50461 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
    50521 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
    50581 tcggttttca ggttatcgga tttagtaccg aaaatagcct gaacaccaga accagcaacc
    50641 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
    50701 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
    50761 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc
    50821 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
    50881 tagtaaacaa tcgcataacc gataccgacg atcggtaaca gccacagttt gctgctgtta
    50941 ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc
    51001 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
    51061 ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag gaacgaggtc
    51121 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
    51181 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt
    51241 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
    51301 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
    51361 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
    51421 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
    51481 gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta
    51541 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
    51601 gctgcaatcg caccggagat aatccctccg agtacgccag tatccgccaa gtgtttagag
    51661 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
    51721 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
    51781 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
    51841 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
    51901 agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc gaccttttgc
    51961 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
    52021 tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
    52081 tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc tatcacgttt
    52141 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
    52201 acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta
    52261 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
    52321 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
    52381 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
    52441 ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag ggggacataa
    52501 cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
    52561 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
    52621 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg
    52681 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
    52741 aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag cccggtttca
    52801 cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
    52861 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
    52921 agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
    52981 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
    53041 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
    53101 aaccggtaac acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
    53161 gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
    53221 ccccagtata gcctgcaggc acgagggaga aagatggttt gccagttcgg cgaccaggcc
    53281 cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg catccatcag
    53341 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag
    53401 cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg tttcacgagc
    53461 ctgccagtta tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg
    53521 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aagtcacggc
    53581 gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
    53641 taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg gctcttcaag
    53701 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
    53761 aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
    53821 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
    53881 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
    53941 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
    54001 cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
    54061 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc
    54121 acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga
    54181 atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg
    54241 aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact
    54301 tccggggtaa catcgagata gagcgttaag tctgggcgaa aatccccgag aacagcatca
    54361 cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg
    54421 gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg tttgatgacc
    54481 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
    54541 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
    54601 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc gagcgtctca
    54661 accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
    54721 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact
    54781 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
    54841 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc
    54901 cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
    54961 agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag
    55021 gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc
    55081 aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt tatctttata
    55141 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
    55201 aactcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg tgtctggcca
    55261 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
    55321 cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc
    55381 gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccaa
    55441 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
    55501 gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc gattgatgat
    55561 cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa
    55621 tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc
    55681 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
    55741 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
    55801 aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
    55861 atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag ctgataagag
    55921 gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
    55981 agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc
    56041 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
    56101 gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg
    56161 cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa
    56221 tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt caatgtgctg
    56281 taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct
    56341 gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagga aatcatcaac
    56401 cgctgacaag catcctgtag tcgcaggata tgcgccgata acaaactgac tttaccgtcg
    56461 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
    56521 agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca gagaattgtt
    56581 agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
    56641 aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag atcaaagaac
    56701 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
    56761 gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg
    56821 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg
    56881 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
    56941 cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
    57001 taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
    57061 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
    57121 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
    57181 gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct
    57241 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
    57301 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
    57361 tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg
    57421 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
    57481 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
    57541 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
    57601 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
    57661 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
    57721 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
    57781 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
    57841 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
    57901 cacgacgctt agacacgttt gtcctccaag gagggaaaaa tgattctagt gggacaaaaa
    57961 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    58021 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    58081 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    58141 caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    58201 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    58261 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    58321 cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc
    58381 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    58441 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    58501 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    58561 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    58621 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    58681 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    58741 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    58801 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttagt
    58861 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    58921 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    58981 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa
    59041 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    59101 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    59161 tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat
    59221 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    59281 agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    59341 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    59401 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa ctgttggtgc
    59461 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    59521 acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag cagctttaca
    59581 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    59641 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    59701 aggcgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg gtacggcttc
    59761 ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacgcc
    59821 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    59881 accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgctggctg
    59941 ggtttgccag gttttattca gttcttcagc tgacccctgc tgggtcagcg cccacaggtc
    60001 gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc
    60061 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    60121 cgtcatgttt taatccttat cctagaaacg aaccagcgca gagccccaag tgaatccacc
    60181 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    60241 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    60301 gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    60361 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    60421 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt
    60481 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    60541 ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag aggcagccag
    60601 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca
    60661 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    60721 gtattgatcg gctacgctta atgcataggt gaaacctgca caggctgctg caacgtcaaa
    60781 tgccgggcaa cctttaatgt ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    60841 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    60901 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    60961 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    61021 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    61081 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    61141 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    61201 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    61261 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    61321 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    61381 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    61441 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    61501 caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    61561 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    61621 ttttaagcac cgctgaggca tcccgaatac tgtcaagacc ctttacttct tcttcaccaa
    61681 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    61741 ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga
    61801 ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa
    61861 tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg
    61921 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata cgcattgaac
    61981 tcccacggct ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    62041 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    62101 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    62161 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    62221 ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    62281 atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg
    62341 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    62401 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    62461 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    62521 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    62581 cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    62641 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    62701 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    62761 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    62821 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    62881 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    62941 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    63001 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    63061 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    63121 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    63181 aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    63241 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc
    63301 gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt cgacgaaacc
    63361 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaatcgcaa
    63421 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    63481 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    63541 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    63601 catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag cgaggcggag
    63661 attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa
    63721 ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac gctggttggt
    63781 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga
    63841 tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg cggcctgatg
    63901 cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg
    63961 cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg
    64021 catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg
    64081 atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc
    64141 gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc
    64201 ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    64261 tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc tttgcagaat
    64321 atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    64381 cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag aacgcaacgc
    64441 cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag
    64501 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa
    64561 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc
    64621 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc
    64681 ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt
    64741 atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca aaatacggta
    64801 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    64861 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg
    64921 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt
    64981 gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca
    65041 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    65101 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    65161 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    65221 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    65281 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    65341 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    65401 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    65461 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    65521 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    65581 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    65641 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    65701 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    65761 atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca
    65821 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccaa
    65881 ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt accgaattaa
    65941 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    66001 gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg aaacactggg
    66061 aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca
    66121 acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa atccttatcg
    66181 ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    66241 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    66301 agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt ggttccattg
    66361 ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc gcgacccgcg
    66421 gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat gagattgctc
    66481 gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc
    66541 cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    66601 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgccagcgcc
    66661 tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta
    66721 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa gaagaagcgc
    66781 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc
    66841 tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt gttcgctgcg
    66901 tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc gtgcgcaaag
    66961 gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    67021 tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca ctggcaacct
    67081 ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga acctgccgcg actgtcgtag
    67141 ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct gggctgttaa
    67201 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc aaaccgaccg
    67261 agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaaacctc
    67321 gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    67381 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag caaactgccg
    67441 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc
    67501 agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac
    67561 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    67621 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    67681 agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact gccgctgccg
    67741 aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc ccgctgccag
    67801 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    67861 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc
    67921 gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt
    67981 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc
    68041 aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga
    68101 ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt gttgaagaaa
    68161 tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa gtggttgaaa
    68221 cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt
    68281 ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accgatggtt gaaccgcagg
    68341 aagagacggc agatgcagat attgaagaag ttgccgaaac tgctgaggtt gtggttgctg
    68401 aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg
    68461 aaacggtggc cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa
    68521 tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt gactggcagc
    68581 gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg
    68641 cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag
    68701 ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca
    68761 aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat
    68821 cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt
    68881 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
    68941 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
    69001 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag
    69061 tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca gcgagttttt
    69121 cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc
    69181 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
    69241 agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc
    69301 gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt
    69361 tccgccaaca taatcaccgt tagcctcact aaacggcgca gtctctgttt tatatgtagc
    69421 gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat
    69481 ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt
    69541 tttcctgagc attctggata gcagtggtga cctggctaag tacactctct tccagtgaca
    69601 ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc tgtgcctggg
    69661 agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg actcgcttac
    69721 ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg atgccgcgca
    69781 tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt
    69841 aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata
    69901 ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg
    69961 ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt
    70021 cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc
    70081 accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttgtc
    70141 gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg tgattaatac
    70201 atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg
    70261 cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg catctggtgt
    70321 cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga
    70381 gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac cgatcgcaac
    70441 gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa agaaatcttc
    70501 accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag cctcggcaaa
    70561 tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag aacggaatgt
    70621 cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc
    70681 cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag gaactgccgc
    70741 cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt gatgttataa
    70801 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    70861 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    70921 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    70981 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    71041 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc cgggtcttcc
    71101 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    71161 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcggtcagg
    71221 ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgtatc atactcacgc
    71281 tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg
    71341 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    71401 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc
    71461 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    71521 gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct
    71581 tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat
    71641 cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc gcggcggtcg
    71701 tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca
    71761 gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    71821 cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg acaccaaaca
    71881 ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcaa
    71941 gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    72001 cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa tcatcgtagt
    72061 tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa cgcaccacgg
    72121 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    72181 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    72241 gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg ctgaacaggc
    72301 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    72361 cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc gcctttagtt
    72421 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    72481 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca aaatagacat
    72541 cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat tgaggctggc
    72601 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    72661 agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    72721 attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    72781 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    72841 attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt tgccacttgg
    72901 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    72961 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    73021 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    73081 gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcctgcgg aggctcctgc
    73141 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    73201 cggagtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg aagaaaccac
    73261 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacactgtca ccattaccgc
    73321 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    73381 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cagtgccatc
    73441 cagccccact accaagccat agccaatcag tgagttttgc cttaccccct gaacactggt
    73501 gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga
    73561 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    73621 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatacgcgca
    73681 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    73741 cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    73801 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    73861 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    73921 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    73981 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    74041 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    74101 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga
    74161 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    74221 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    74281 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttatgcctta
    74341 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    74401 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    74461 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    74521 tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca gtccggtatc
    74581 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    74641 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    74701 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    74761 gaaagagccg tcacggatat aggctgatga gccatctggc aacattacct ggaaaaagcc
    74821 ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc cctggctgtg
    74881 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    74941 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    75001 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    75061 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    75121 ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag tgtgaatttc
    75181 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    75241 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    75301 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    75361 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    75421 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    75481 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    75541 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    75601 gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc tatcaccgga
    75661 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    75721 ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca acgtccagcg
    75781 ggcgagaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta
    75841 ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag gcgtttaact
    75901 gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc gcctgttgat
    75961 tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt gagctatccc
    76021 gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt tttgatggtc
    76081 tgggcgttag acttatagtt acgctgggca acgatcatat tgaccagttc tttactgaga
    76141 tcgacgttgg acgcttccag cgcaccgttg gtcagagtgc caaagtttcc cgtcccggct
    76201 gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc gccttcggat
    76261 gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag ttgggtttgt
    76321 tcgttggaat tgttgccgac aaccgtaccg tcatcattga tttgataact caccagatcg
    76381 cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt attttgctgc
    76441 atggagttga ggaagctcag actaaacgtg gcgggttctg caccgttaat tgcgccggtt
    76501 gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa ttccagtgtt
    76561 gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac ctgccagtta
    76621 ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg actgtcgaaa
    76681 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca
    76741 gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    76801 gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    76861 ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac cttgcgtatt caacaggtta
    76921 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    76981 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    77041 gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc
    77101 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    77161 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    77221 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    77281 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct gttgccgcgg
    77341 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    77401 ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc agtcaacgaa
    77461 ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat ggtgcgcaca
    77521 accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca
    77581 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc cgcaagaaca
    77641 gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    77701 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccgctgacc
    77761 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    77821 tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    77881 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg
    77941 gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    78001 tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg
    78061 tgttaaccat ctccccgacg acatcaacgt tcggcatttt tatgtagccc tttgcatctg
    78121 ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct taacatcggc
    78181 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    78241 tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca gattactggc
    78301 cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    78361 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc
    78421 gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc
    78481 gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac ggtattgcag
    78541 ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc tgcgttgagg tcatcgtcag
    78601 tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa
    78661 atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac
    78721 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    78781 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    78841 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    78901 ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    78961 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga gcaatctcac
    79021 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    79081 ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc tttcgatgag
    79141 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    79201 atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga tgcctattgt
    79261 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc tggataccct
    79321 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    79381 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    79441 acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    79501 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg aacaggtagt
    79561 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    79621 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    79681 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    79741 caccgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    79801 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    79861 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    79921 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta actgatcgta
    79981 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    80041 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    80101 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    80161 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    80221 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    80281 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    80341 gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg atctgaatat
    80401 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    80461 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    80521 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    80581 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    80641 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    80701 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    80761 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    80821 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    80881 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    80941 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    81001 gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    81061 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    81121 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    81181 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    81241 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctcca
    81301 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    81361 tgatacacca gctgcagaat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    81421 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    81481 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    81541 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    81601 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    81661 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    81721 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    81781 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    81841 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    81901 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    81961 aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct
    82021 gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat gttacagggt
    82081 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgccta
    82141 ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat tcaatgaagt gccgcgcaca
    82201 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tattcactac
    82261 accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    82321 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aagtggtgat
    82381 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    82441 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    82501 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    82561 acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta actcaccgta
    82621 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    82681 ttccaccagc ctttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    82741 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac
    82801 aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    82861 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggctcctt gtaacgtcca
    82921 gtcagacgct gccgccagca ccggtaacca cgctttttgc acaatgccgc ctaatcccac
    82981 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    83041 gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt
    83101 caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca ctgaaaagat
    83161 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    83221 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    83281 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    83341 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    83401 aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt agataatgac
    83461 gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc atgaccggtt
    83521 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    83581 ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc agggcagtaa
    83641 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    83701 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    83761 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    83821 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    83881 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    83941 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    84001 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    84061 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    84121 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    84181 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    84241 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    84301 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    84361 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatactgtcc ccgaagtgat
    84421 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    84481 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    84541 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    84601 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    84661 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    84721 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    84781 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    84841 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    84901 ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag ctggctgttg
    84961 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg
    85021 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    85081 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    85141 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    85201 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    85261 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    85321 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctacaacaa
    85381 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    85441 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    85501 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    85561 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    85621 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    85681 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga actgacagcc
    85741 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    85801 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    85861 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    85921 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    85981 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    86041 tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    86101 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    86161 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    86221 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    86281 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    86341 aattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    86401 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    86461 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    86521 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    86581 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    86641 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    86701 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    86761 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    86821 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    86881 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    86941 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    87001 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    87061 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    87121 ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca aaaacatccc
    87181 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgccc
    87241 agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac
    87301 cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg tgaccacagt
    87361 tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg ggcacgattt
    87421 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    87481 atttaatgaa gccgtgttca ccgctgcaaa actttacccg gcaaacgcaa ccactaactc
    87541 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    87601 cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca tttttgatcg
    87661 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    87721 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    87781 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    87841 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    87901 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    87961 gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    88021 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    88081 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    88141 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    88201 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    88261 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    88321 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    88381 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    88441 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    88501 aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca
    88561 gcgaataata tgcagtgatt tttgtttttg ccggatcgcc ccggcttttt tattttactt
    88621 acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat atgctaaaaa
    88681 aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa atttatctgt
    88741 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
    88801 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
    88861 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
    88921 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
    88981 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
    89041 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
    89101 cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
    89161 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
    89221 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
    89281 tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
    89341 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
    89401 aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac
    89461 gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc tgactccgca
    89521 tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga aaagctcgat
    89581 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta catcggctta
    89641 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
    89701 gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac
    89761 gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc
    89821 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa
    89881 gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt cccggcgttt
    89941 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
    90001 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
    90061 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
    90121 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
    90181 attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct gagtgggcac
    90241 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agaccaaaaa
    90301 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
    90361 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
    90421 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
    90481 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
    90541 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    90601 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
    90661 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
    90721 cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa
    90781 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
    90841 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
    90901 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
    90961 tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat
    91021 gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt tcgccatcgg
    91081 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
    91141 tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca
    91201 tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta tggccctgaa
    91261 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
    91321 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt
    91381 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
    91441 tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct ttaaagattt
    91501 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
    91561 taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga
    91621 ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
    91681 cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
    91741 gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt
    91801 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
    91861 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
    91921 tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag gaatacccag
    91981 tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
    92041 ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca
    92101 acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt ggcagccatc
    92161 atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat
    92221 ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
    92281 caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg
    92341 ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
    92401 gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
    92461 tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac
    92521 ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc
    92581 gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc
    92641 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
    92701 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
    92761 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
    92821 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
    92881 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
    92941 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
    93001 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
    93061 ttatcgataa ttaacatcca catattttac gctacatttg cgcattaaaa attatttgtt
    93121 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
    93181 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
    93241 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
    93301 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
    93361 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
    93421 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
    93481 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
    93541 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
    93601 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
    93661 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
    93721 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
    93781 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg
    93841 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
    93901 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
    93961 agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct ccgccactgg
    94021 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
    94081 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
    94141 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
    94201 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
    94261 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
    94321 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
    94381 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
    94441 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
    94501 tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag
    94561 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
    94621 gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt
    94681 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
    94741 tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt
    94801 agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag
    94861 ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa
    94921 agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt
    94981 gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg
    95041 tatcaaggta tctaggcgtt gtcctgca
//
LOCUS       DAJVPT010000002        77884 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVPT010000002 DAJVPT010000000
VERSION     DAJVPT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..428)
                     /locus_tag="OQM47_000107"
     CDS             complement(<1..428)
                     /locus_tag="OQM47_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCS1478409.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFN"
     gene            complement(562..1659)
                     /gene="cysA"
                     /locus_tag="OQM47_000108"
     CDS             complement(562..1659)
                     /gene="cysA"
                     /locus_tag="OQM47_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1478410.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(1649..2524)
                     /gene="cysW"
                     /locus_tag="OQM47_000109"
     CDS             complement(1649..2524)
                     /gene="cysW"
                     /locus_tag="OQM47_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1478411.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFYFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(2524..3357)
                     /gene="cysT"
                     /locus_tag="OQM47_000110"
     CDS             complement(2524..3357)
                     /gene="cysT"
                     /locus_tag="OQM47_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1478412.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(3357..4373)
                     /gene="cysP"
                     /locus_tag="OQM47_000111"
     CDS             complement(3357..4373)
                     /gene="cysP"
                     /locus_tag="OQM47_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1478413.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(4677..5468)
                     /gene="ucpA"
                     /locus_tag="OQM47_000112"
     CDS             complement(4677..5468)
                     /gene="ucpA"
                     /locus_tag="OQM47_000112"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1478414.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(5655..6455)
                     /gene="murR"
                     /locus_tag="OQM47_000113"
     CDS             complement(5655..6455)
                     /gene="murR"
                     /locus_tag="OQM47_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1478415.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            6619..7515
                     /gene="murQ"
                     /locus_tag="OQM47_000114"
     CDS             6619..7515
                     /gene="murQ"
                     /locus_tag="OQM47_000114"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1478416.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            7519..8943
                     /gene="murP"
                     /locus_tag="OQM47_000115"
     CDS             7519..8943
                     /gene="murP"
                     /locus_tag="OQM47_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1478417.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(9123..10022)
                     /gene="yfeX"
                     /locus_tag="OQM47_000116"
     CDS             complement(9123..10022)
                     /gene="yfeX"
                     /locus_tag="OQM47_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1478418.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(10118..10693)
                     /locus_tag="OQM47_000117"
     CDS             complement(10118..10693)
                     /locus_tag="OQM47_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1478419.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(10754..11203)
                     /locus_tag="OQM47_000118"
     CDS             complement(10754..11203)
                     /locus_tag="OQM47_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1478420.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(11190..11615)
                     /locus_tag="OQM47_000119"
     CDS             complement(11190..11615)
                     /locus_tag="OQM47_000119"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1478421.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11829..12698
                     /gene="amiA"
                     /locus_tag="OQM47_000120"
     CDS             11829..12698
                     /gene="amiA"
                     /locus_tag="OQM47_000120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1478422.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            12702..13601
                     /gene="hemF"
                     /locus_tag="OQM47_000121"
     CDS             12702..13601
                     /gene="hemF"
                     /locus_tag="OQM47_000121"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1478423.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(13607..14659)
                     /gene="eutR"
                     /locus_tag="OQM47_000122"
                     /pseudo
     CDS             complement(13607..14659)
                     /gene="eutR"
                     /locus_tag="OQM47_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(14705..15205)
                     /gene="eutK"
                     /locus_tag="OQM47_000123"
     CDS             complement(14705..15205)
                     /gene="eutK"
                     /locus_tag="OQM47_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1478424.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(15218..15877)
                     /gene="eutL"
                     /locus_tag="OQM47_000124"
     CDS             complement(15218..15877)
                     /gene="eutL"
                     /locus_tag="OQM47_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1478425.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            17059..17850
                     /locus_tag="OQM47_000125"
     CDS             17059..17850
                     /locus_tag="OQM47_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478426.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            18570..18965
                     /locus_tag="OQM47_000126"
     CDS             18570..18965
                     /locus_tag="OQM47_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1478427.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            18983..19246
                     /locus_tag="OQM47_000127"
     CDS             18983..19246
                     /locus_tag="OQM47_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478428.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            19269..19811
                     /locus_tag="OQM47_000128"
     CDS             19269..19811
                     /locus_tag="OQM47_000128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478429.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            19950..20237
                     /locus_tag="OQM47_000129"
     CDS             19950..20237
                     /locus_tag="OQM47_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478430.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            20276..20482
                     /locus_tag="OQM47_000130"
     CDS             20276..20482
                     /locus_tag="OQM47_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478431.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            20794..21279
                     /locus_tag="OQM47_000131"
     CDS             20794..21279
                     /locus_tag="OQM47_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1478432.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            21260..21538
                     /locus_tag="OQM47_000132"
     CDS             21260..21538
                     /locus_tag="OQM47_000132"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478433.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            21531..22991
                     /locus_tag="OQM47_000133"
     CDS             21531..22991
                     /locus_tag="OQM47_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1478434.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            23025..23834
                     /gene="murI"
                     /locus_tag="OQM47_000134"
     CDS             23025..23834
                     /gene="murI"
                     /locus_tag="OQM47_000134"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1478435.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(23953..24647)
                     /locus_tag="OQM47_000135"
     CDS             complement(join(23953..24401,24401..24647))
                     /locus_tag="OQM47_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1478436.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(25166..26539)
                     /gene="eutA"
                     /locus_tag="OQM47_000136"
     CDS             complement(25166..26539)
                     /gene="eutA"
                     /locus_tag="OQM47_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1478437.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(26514..26993)
                     /gene="eutP"
                     /locus_tag="OQM47_000137"
     CDS             complement(26514..26993)
                     /gene="eutP"
                     /locus_tag="OQM47_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1478438.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(27006..27341)
                     /gene="eutS"
                     /locus_tag="OQM47_000138"
     CDS             complement(27006..27341)
                     /gene="eutS"
                     /locus_tag="OQM47_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1478439.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(27634..29790)
                     /gene="maeB"
                     /locus_tag="OQM47_000139"
                     /pseudo
     CDS             complement(27634..29790)
                     /gene="maeB"
                     /locus_tag="OQM47_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
     gene            29787..30443
                     /locus_tag="OQM47_000140"
                     /pseudo
     CDS             29787..30443
                     /locus_tag="OQM47_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase A"
     gene            30463..32466
                     /gene="tkt"
                     /locus_tag="OQM47_000141"
     CDS             30463..32466
                     /gene="tkt"
                     /locus_tag="OQM47_000141"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1478440.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(32569..33612)
                     /locus_tag="OQM47_000142"
     CDS             complement(32569..33612)
                     /locus_tag="OQM47_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1478441.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(33738..34313)
                     /gene="nudK"
                     /locus_tag="OQM47_000143"
     CDS             complement(33738..34313)
                     /gene="nudK"
                     /locus_tag="OQM47_000143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1478442.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(34381..36360)
                     /gene="aegA"
                     /locus_tag="OQM47_000144"
     CDS             complement(34381..36360)
                     /gene="aegA"
                     /locus_tag="OQM47_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1478443.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            36566..38283
                     /gene="narQ"
                     /locus_tag="OQM47_000145"
                     /pseudo
     CDS             36566..38283
                     /gene="narQ"
                     /locus_tag="OQM47_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            38485..41598
                     /gene="acrD"
                     /locus_tag="OQM47_000146"
     CDS             38485..41598
                     /gene="acrD"
                     /locus_tag="OQM47_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1478444.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(41697..41756)
                     /gene="ypfM"
                     /locus_tag="OQM47_000147"
     CDS             complement(41697..41756)
                     /gene="ypfM"
                     /locus_tag="OQM47_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1478445.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            42137..42493
                     /locus_tag="OQM47_000148"
     CDS             42137..42493
                     /locus_tag="OQM47_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1478446.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            42497..43624
                     /gene="dapE"
                     /locus_tag="OQM47_000149"
     CDS             42497..43624
                     /gene="dapE"
                     /locus_tag="OQM47_000149"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1478447.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            43652..43852
                     /locus_tag="OQM47_000150"
     CDS             43652..43852
                     /locus_tag="OQM47_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1478448.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(43962..44660)
                     /gene="ypfH"
                     /locus_tag="OQM47_000151"
     CDS             complement(43962..44660)
                     /gene="ypfH"
                     /locus_tag="OQM47_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1478449.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(44734..46749)
                     /gene="tmcA"
                     /locus_tag="OQM47_000152"
     CDS             complement(44734..46749)
                     /gene="tmcA"
                     /locus_tag="OQM47_000152"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1478450.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(46764..47627)
                     /locus_tag="OQM47_000153"
     CDS             complement(46764..47627)
                     /locus_tag="OQM47_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1478451.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(47795..48508)
                     /gene="purC"
                     /locus_tag="OQM47_000154"
     CDS             complement(47795..48508)
                     /gene="purC"
                     /locus_tag="OQM47_000154"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1478452.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(48721..49755)
                     /gene="bamC"
                     /locus_tag="OQM47_000155"
     CDS             complement(48721..49755)
                     /gene="bamC"
                     /locus_tag="OQM47_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1478453.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(49772..50650)
                     /gene="dapA"
                     /locus_tag="OQM47_000156"
     CDS             complement(49772..50650)
                     /gene="dapA"
                     /locus_tag="OQM47_000156"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1478454.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            50796..51368
                     /locus_tag="OQM47_000157"
     CDS             50796..51368
                     /locus_tag="OQM47_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1478455.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            51368..51838
                     /gene="bcp"
                     /locus_tag="OQM47_000158"
     CDS             51368..51838
                     /gene="bcp"
                     /locus_tag="OQM47_000158"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1478456.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            52091..52708
                     /gene="hyfA"
                     /locus_tag="OQM47_000159"
     CDS             52091..52708
                     /gene="hyfA"
                     /locus_tag="OQM47_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1478457.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            52708..54725
                     /gene="hyfB"
                     /locus_tag="OQM47_000160"
                     /pseudo
     CDS             52708..54725
                     /gene="hyfB"
                     /locus_tag="OQM47_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            54736..55683
                     /gene="hyfC"
                     /locus_tag="OQM47_000161"
     CDS             54736..55683
                     /gene="hyfC"
                     /locus_tag="OQM47_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1478458.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            55700..57139
                     /gene="hyfD"
                     /locus_tag="OQM47_000162"
     CDS             55700..57139
                     /gene="hyfD"
                     /locus_tag="OQM47_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1478459.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            57151..57801
                     /gene="hyfE"
                     /locus_tag="OQM47_000163"
     CDS             57151..57801
                     /gene="hyfE"
                     /locus_tag="OQM47_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1478460.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            57806..59398
                     /gene="hyfF"
                     /locus_tag="OQM47_000164"
     CDS             57806..59398
                     /gene="hyfF"
                     /locus_tag="OQM47_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1478461.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            59388..61102
                     /gene="hyfG"
                     /locus_tag="OQM47_000165"
                     /pseudo
     CDS             59388..61102
                     /gene="hyfG"
                     /locus_tag="OQM47_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            61112..61657
                     /gene="hyfH"
                     /locus_tag="OQM47_000166"
     CDS             61112..61657
                     /gene="hyfH"
                     /locus_tag="OQM47_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1478462.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            61654..62412
                     /gene="hyfI"
                     /locus_tag="OQM47_000167"
     CDS             61654..62412
                     /gene="hyfI"
                     /locus_tag="OQM47_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1478463.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            62405..62818
                     /gene="hyfJ"
                     /locus_tag="OQM47_000168"
     CDS             62405..62818
                     /gene="hyfJ"
                     /locus_tag="OQM47_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1478464.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            62848..64677
                     /gene="hyfR"
                     /locus_tag="OQM47_000169"
     CDS             62848..64677
                     /gene="hyfR"
                     /locus_tag="OQM47_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1478465.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            64678..64860
                     /locus_tag="OQM47_000170"
     CDS             64678..64860
                     /locus_tag="OQM47_000170"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCS1478466.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            64882..65754
                     /locus_tag="OQM47_000171"
                     /pseudo
     CDS             64882..65754
                     /locus_tag="OQM47_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(65792..66853)
                     /locus_tag="OQM47_000172"
     CDS             complement(65792..66853)
                     /locus_tag="OQM47_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1478467.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            67066..68529
                     /gene="bepA"
                     /locus_tag="OQM47_000173"
     CDS             67066..68529
                     /gene="bepA"
                     /locus_tag="OQM47_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1478468.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            68550..68909
                     /gene="arsC"
                     /locus_tag="OQM47_000174"
     CDS             68550..68909
                     /gene="arsC"
                     /locus_tag="OQM47_000174"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1478469.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(69042..69788)
                     /gene="hda"
                     /locus_tag="OQM47_000175"
     CDS             complement(69042..69788)
                     /gene="hda"
                     /locus_tag="OQM47_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1478470.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(69838..71127)
                     /gene="uraA"
                     /locus_tag="OQM47_000176"
     CDS             complement(69838..71127)
                     /gene="uraA"
                     /locus_tag="OQM47_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1478471.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(71213..71839)
                     /gene="upp"
                     /locus_tag="OQM47_000177"
     CDS             complement(71213..71839)
                     /gene="upp"
                     /locus_tag="OQM47_000177"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1478472.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            72163..73200
                     /gene="purM"
                     /locus_tag="OQM47_000178"
     CDS             72163..73200
                     /gene="purM"
                     /locus_tag="OQM47_000178"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1478473.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            73200..73838
                     /gene="purN"
                     /locus_tag="OQM47_000179"
     CDS             73200..73838
                     /gene="purN"
                     /locus_tag="OQM47_000179"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1478474.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            74010..76076
                     /gene="ppk1"
                     /locus_tag="OQM47_000180"
     CDS             74010..76076
                     /gene="ppk1"
                     /locus_tag="OQM47_000180"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1478475.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            76081..77622
                     /gene="ppx"
                     /locus_tag="OQM47_000181"
     CDS             76081..77622
                     /gene="ppx"
                     /locus_tag="OQM47_000181"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1478476.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(77661..>77884)
                     /locus_tag="OQM47_000182"
     CDS             complement(77661..>77884)
                     /locus_tag="OQM47_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1478477.1"
                     /translation="NIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLG
                     IDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        18699 a        20420 c        19790 g        18975 t
ORIGIN      
        1 ttattgaact gatcgagcag ctttccttcg ccacgattcg ccatctccag cgccagatcg
       61 cgcgcacctt ccatgccctg ctctttggtg acaagaatca gttccgcacc ataagcacgc
      121 atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat gcgatagcct
      181 ttcagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc ttcgattaag
      241 acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga aagtgccgca
      301 cgatctttca ccgaacctgc cgggttattg ccttccagtt ttaaccacac ttcactgccg
      361 ttatccggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt ttgttctaat
      421 gtactcacaa tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca
      481 ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc gcctccgggc
      541 gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt
      601 ttcaatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga ataaacgctc
      661 gccatgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt tgtaccaccc
      721 cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca gtacctgtac
      781 cggcagcggc gaatcgagac tggtacggcg gctgatatcc acttcccacg ggcgcaggaa
      841 gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat gcgcgccaac
      901 atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca taaattcgag
      961 tacaaaacgg gtcgctggtt cgcgccatac ctgattcggc gcatcagcct gttcaatatt
     1021 cccctgactc atcaccacta cacgatcggc tacttcggtc gcctcttcct ggtcgtgggt
     1081 cacaaacacg ctggtgaatt tcagctcttc atgcagttga cgcagccagc gacgcagctc
     1141 tttacgcacc tgcgcatcca gcgcgccgaa cggttcatca agcagcagaa tttgcggttc
     1201 tacagccagc gcgcgcgcca gcgccacacg ctgtttctgg ccgccggaaa gctgcgccgg
     1261 ataacgatcc gccagatgcg caagctggac catttccagc aattttgtca ctttcgcttt
     1321 gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa aagtgatatt
     1381 gtcgaatacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac cgactttacg
     1441 atcgcgcgcg tgcaggcgac tcacgtcggt gccgtggaag cgaatatgcc cgctggtttg
     1501 atgctccagc ccggcgataa tccgcagcag cgtggttttc ccggaaccgg acggccccag
     1561 caacgcgacc atctgaccag aaggaatatc cagtgagata tcgttcagca cctgggtgcg
     1621 accaaaagac ttcttgatat tggcaatctc aatgctcatg atgctcctcc tgctgcgcgc
     1681 gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg gtgataatcg
     1741 ccatcaacgt caacagcgcg gcggcggtaa aggagccgac ggtgttgtag tcctgctcca
     1801 acaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg gaaaccaccg
     1861 acactgcacc aaactcgcca atcgcgcggg cgttggtcag caccacgccg taaagcagcg
     1921 cccagcgaat attcggcaat gtgacgcgac ggaacatctg ccagccggac gcgccaagca
     1981 aaatcgccgc ttcgtcttcc tggctgccct ggcttaacat caccggcacc agctcgcgca
     2041 ccacaaacgg acacgtcaca aagatggtga ccagcaccat tccgggccag gagaacataa
     2101 tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg tagaacagca
     2161 gatacaccag accggcaacc accggcgata cagcaaacgg aatgtccagt agcgtcagca
     2221 gtaactggcg tcccgggaag taaaagcgcg tcaccagcca ggccagcaga atgccgaaca
     2281 ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg gcgtgcagca
     2341 tgtccggatc ggccagattc tgtaaaaccg gcatcagccc cttgctgaat gcctgcacga
     2401 agatgtaaat catcggcacc agcaggatga acgccgaaac cagcatcccg atgccaatca
     2461 gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgg gtaacttccg
     2521 ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag
     2581 cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgctgccg ggtaatcaaa
     2641 ctcctgtaag cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg cgatatttcc
     2701 ggcgataaaa atcaccgcgc caaattcacc aagactacgg gtaaacgaca gcgccacgcc
     2761 cgccaccagc gccggagaaa gctccggcag caccactttg cagaaactct gccagcgcgt
     2821 tgcaccaagc gtttccgccg cttcttcata ttccgggcct aactcttcca gcaccggctg
     2881 cacggtacgc accacaaacg gaatgctggt aaaggccatc gccaccgcaa taccaagcca
     2941 ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac cgtttacgga
     3001 aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat
     3061 cagcgcatca agcagcgtgc ggcccggaaa gcgataacgt gttaaaatcc acgccatcag
     3121 cagaccgaaa acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg tcactttgta
     3181 ggccgcgacc acctgcgggt tggtgatcac ctcccagtac tgcgcccagc tcatctcaga
     3241 aagctgcatc accagcgcag agagcggcag cagcaatatc aggcacacaa acagcagact
     3301 ggtgccaagg cttaaagtaa agcccggcag cacgcgtctt gaagaaactg caaacattac
     3361 ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc
     3421 acttccggcc atgagccaaa tttgtcttcc acgcggaaca gttcggtctg cgggaattta
     3481 tctttcagtt tgtccatgac ttccgggtta ttcacacggt agtaatagtc ggtgatgatg
     3541 gtctgcgcct gtgggctgta gagccagttc aggtaggctt ttgcggcttt ttccgtaccg
     3601 ttggcctgca catttttatc gacccacgcc accgggaatt ccgccagaat attggttttc
     3661 gggatcacca cttcaaagcc ctgtgcttca tactgtttac ggatgttgtt cacttccgat
     3721 tcaaagctaa tcagcacatc gcccaagcca cgctcggcaa aagtggtggt tgcgccacga
     3781 ccgccggtat cgaacacttc aacgtttttc aggaactggg tcataaactg ttcggttttg
     3841 gctttatcgc caccgtcagc tttatccgct gcgccccatg ccgccagata ggtataacgc
     3901 gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg caccaggtcg
     3961 ttccagtcgt ggatattctt cgggttgccc ttacgcacca ggaagcccat ggtggagtag
     4021 aacggcgagc tattattcgg caggcgcgac tgccagtcag ccgggatcag cttgcctttg
     4081 tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtctgc ttttaagccc
     4141 tgcaaaatcg ccagcgcctg ttttgatgac ccggcatgag attgttttat cgtcagtttg
     4201 tcgccgccgt tatcttttgc ccattgctgt tcaaacggcg gattcagggc ggcaaacagc
     4261 tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagc
     4321 agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc cattgcgcac
     4381 ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta
     4441 acggttttat ataccgtttg gtgatttgga agttaaaaag ggaataagaa cttggttcaa
     4501 caggcgtaat tgttgcaggc agtttggaca cggacagcgc ggagaaaccg gagcgtacac
     4561 atagtacgtg aggatttcga gcactgcccg gggccaaaat gacaaatgaa atagcctggt
     4621 gaacttagtt caagacgaaa tcctccccac acatgcaggg aggaaacaga ggtgaatcag
     4681 ataccgacgc taaccgtctc cggcagtgtg ctgccgccat caatcacatt ctgtgtaccg
     4741 gttaaatagc tggattcatc cgatgcgagg aaggccgcca gttcgccgac ttccagcgga
     4801 tcggcgaggc gacacagcgg gattgctttt gccatttcag tcagcaccga ttctggatct
     4861 tccgggttcg actggcgggc aatgctttcc gccattggcg tgcggacgta tcccgggcag
     4921 atggcgttaa cgcgaatacc ggattgcgcg tactccaccg ccagcgattt agtcaggcca
     4981 acaatcgccg ctttcgttaa ggcatacgcc gtttcgccag gatcggccac catatcacca
     5041 gtgactgaag acatcatcac aatgcgacca tctttgcgcg caatcatctc cggcagcacc
     5101 gccttcgtga cgttccatac gcctttaata ttgatatcaa tatggaaatc gcgatcttca
     5161 tcgctcatat cgaggaaact gcccagacga caaacgcctg cgttattcac caggatatca
     5221 atgcgccctt ctttttcctt cgcgcgtttg atagctgcgg ctaccgacgc cgggtcacgc
     5281 acatcggcga caaccgccgt acagcgatga ccacgaccac acagttcgtc cgctagcttt
     5341 tcgatctcag gggagatatc cagcaagatt aggttcgcgc catgacgtgc aaaagttctg
     5401 gcaattcctt cgccaattcc ctgcaatgcg cccgtaatca gtgctgtctt gcccgtgagt
     5461 ttacccattt taatgctctc cttgttaata cagagcgtta cactccattc cctctgaaaa
     5521 tacaggataa acaaaatatt gctgcggatc agctcactaa ttcaggaaaa tctcaatcca
     5581 gtcgctgctt tatgcatcat ttcaggcgtt gtgttagctc actgctgcgc tgaatcattt
     5641 tcaatgacgc cacatcattg atgttgcaca agaccaacaa acagtaaatc ggtaacagaa
     5701 ttttgcgccg tgcgggtaga catcgacgag ctacgccatt cggtttcacc ggaaacggta
     5761 tcgagcgtga aatgcgccag ccgccgcagc ggagagtcag ccagagaagt aatggcaatc
     5821 accgttgccc cctgctttcg tgccgcttct gcgcacaaga caatctcttt tttactgccg
     5881 ctgtaagaga tagcgatttg tacatcgcct tttttcaacg cctgggaaac ggtggcttgt
     5941 acgtgcgtgt cggcttcgca ggccacgcga tagccaattt tcatcagttt gaacgacaga
     6001 tcgcgcccca ccagcgccga tccgcccagg cccgttatct ggataaacgg tgctttactg
     6061 atgacctcaa tgattttttg cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc
     6121 gccagctctt tttcgcgatt cagtttgcgg gcaatcactt ccagcgaatc atcactggta
     6181 atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc actgtattcg
     6241 ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg ggcaaatttc
     6301 acaatgctcg actggctgat accgagctgt ttcgccatct gccgtgaaga gacggatttc
     6361 agctcgctca ctctggcccg cagaaagtca gcaatttttt gctcgttccc ggtaaattca
     6421 ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca
     6481 aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt
     6541 cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa
     6601 atagtaaggt caccaccgat gcaactagaa aagatgatta ctgaaggctc gaacaccgcc
     6661 tcggctgaaa ttgaccgcgt atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat
     6721 aaaaccgtac cgcctgccgt tgatcgcgtc ctgccggata tcgccgcggc gatcgatgtt
     6781 atccacgccc aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt
     6841 cgtctgggga ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa accgggtctg
     6901 gtggttggtt tgattgctgg aggcgaatat gccattcagc acgcggtgga aggagctgaa
     6961 gatagccggg aaggtggtgt taacgatctg aaaaatatta atttaacggc acaggatgtg
     7021 gtggttggca ttgctgccag cggtcgcacg ccgtatgtga ttgccggact ggaatacgca
     7081 cgccagctcg gctgccgcac agtgggaatt tcctgtaatc cggggagcgc cgtttcaacc
     7141 accgctgagt ttgccattac accgattgta ggtgccgaag ttgttaccgg ttcttcgcgg
     7201 atgaaagcag gtacagcgca gaaactggtg ctcaatatgc tttccaccgg gctgatgatt
     7261 aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg
     7321 catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtaatgccga acaagcggaa
     7381 gcggcgttaa ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat
     7441 ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt
     7501 ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa caccattctt
     7561 acccgtgtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt
     7621 ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga aggcgtgaag
     7681 ggcgtcattt tgaccagcga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt
     7741 gccgcaaaag cgatgagcga gctgctgggc gatgcaccgg tacaggatgc cgcagaaatc
     7801 gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc
     7861 gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc cggtctgctg
     7921 ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg
     7981 ctacccgacg cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac cttcctagtg
     8041 attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt
     8101 attgccgcac tctttttgct cggttataac cccgctgcaa ccaccggtta ctacgccggt
     8161 tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgctgatt
     8221 gccgcgtggg cctgcgcccg cattgaaggc atggtgcgcc gctttatgcc ggacgatctc
     8281 gacatgctgc tgacctcgtt aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc
     8341 attatgccgc tgggtggctg gttattcgaa ggtatgtcat ggctgtttat gcacctgaac
     8401 agtaatccgt tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt ggtatttggc
     8461 gtgcatcagg gctttattcc tgtttacctc gcgttaatgg acagccaggg attcaacagc
     8521 ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac
     8581 tggcgggcgc aaccgcacag tgcgctacgc agtcaggtac gcggggcgat tattcccggc
     8641 ctgctgggcg ttggtgaacc gctgatttac ggtgtcaccc tgccccgcat gaagccgttt
     8701 gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta tcggattgat agcctggtgg
     8761 ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg
     8821 atgacttccg cacaaggcat ccttccggca atggcggttt atgctggcgg gattctggtg
     8881 gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt caatctggac
     8941 tgatattatg aaacggacaa tgctctatct ttctctgctg gctgttagtt gtagcgtcag
     9001 cgccgcaaaa taccctgttc tgacagaagg tagttgatca agtatatgcg gcgttaaagc
     9061 agtaataaaa aggccagtca gtaatgttac tgcctggcct ttaatcaatg aatcagaaac
     9121 gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc ggttaccggt
     9181 ttggtgaaac gcaacatcgc atcacgtttg ccatcggtat cgccaaacat gctcagcagt
     9241 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
     9301 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
     9361 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
     9421 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
     9481 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggaatc cacgccgtct
     9541 ttgataaccg ccacttcgcg gcgcgtctct tcacccgccg ggttttctgt accgtcaaca
     9601 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
     9661 atgcagtcac caaaggcttc catcgccgcc tgagcgacag agaagtttac gtcgtgacgc
     9721 agagaaagaa tgtggatcaa cacatcgaac tgggtcgtcg gcgcaaggcc tttaccgtag
     9781 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
     9841 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
     9901 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
     9961 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    10021 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    10081 cgtgtttttt cgcgacggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    10141 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    10201 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    10261 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    10321 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    10381 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    10441 aaaagcgcac cacgttgccg ttcgtggttt tcattccgct gcgcaggcga taatcgccat
    10501 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    10561 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gcagaccagt
    10621 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    10681 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    10741 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    10801 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    10861 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    10921 acagaaaata gagcacatgc caggtgcgag gaaacgaagc ccgccgaccg ctcaacaaaa
    10981 acgccagcac cgccggaata ccgggaatca gccccagcca gaaattatcg tgatcgggat
    11041 aaaacagatt tagcagcgca gtaccctgct cacgcgacgc accggcaatg acaaacagca
    11101 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    11161 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    11221 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    11281 tcgttatctt ccggcacgtt gatctgaatt ttcgggcagc cgcgagcaat cagctttttc
    11341 tccagccggt taagcaacgc attggcaatt ccacgcccac gaaactctgg atgcacgcca
    11401 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    11461 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    11521 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    11581 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    11641 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    11701 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacag
    11761 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    11821 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    11881 aagccggttt agctgccctg acgttgtcag gaatgtcgca agccattgcc aaagacgaac
    11941 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    12001 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    12061 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    12121 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    12181 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    12241 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    12301 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    12361 cgactgacaa ggatcaccta ttgcagcaag tactgtttga tctggtgcaa accgatacta
    12421 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    12481 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    12541 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    12601 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    12661 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    12721 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    12781 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgcagtc
    12841 gggtgttgcg taatggtggt gttttcgaac aggcgggcgt caacttttct cacgtccacg
    12901 gtgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    12961 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    13021 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtttgg tggtttggcg
    13081 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    13141 ccgcccgtga cctgtgccag ccatttggtg aagacgttta tccccgttac aaaaagtggt
    13201 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggcattggc gggctgttct
    13261 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    13321 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    13381 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    13441 gggatcgcgg cacgctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    13501 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    13561 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    13621 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    13681 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    13741 gcgaccacgg gcttatcagt tcgcggcgta cgacgttcag gcgaatgcgt ttcagccacg
    13801 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    13861 gcagttgatt acacaaatcc agcaccgtca ccggttcgga catgttttcc agcacatatt
    13921 cacgggcgcg ggaaagcaac cgacggtaac tctgatgact gatgctttcc gccgtcacca
    13981 ttggttgcgc ttcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    14041 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    14101 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    14161 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacag gtgatgacat
    14221 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    14281 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    14341 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    14401 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagt agaatttccg
    14461 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc taggcggtaa
    14521 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    14581 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    14641 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    14701 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    14761 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcggccactt
    14821 ctcctgccgt cattccctga catactgatg ttaacagcgc caacaactcg tcagcagatt
    14881 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    14941 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttacgg ctgatgacac
    15001 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    15061 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    15121 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    15181 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    15241 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    15301 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggacg taggtcacca
    15361 gctgtacgtc ggcagatttc aacgcggcat caatgccgta ggtcgcttcc agcggcggtg
    15421 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    15481 cggtacgcga aaccacatgc gccaggaagg cggtgttttc tgcgtcattg gcccactgga
    15541 aagctgcgcc attttcaata tgtgcaacca tcgcatccag accggcacgc acttccgccg
    15601 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggg ccgtgtgccg
    15661 ctcccgcata cagcgagcga ccatatacca cttcgaccat cgcttgtttg gtcgcttcgt
    15721 cagccgcaat gtaggttacg tcgtcagaat cggcagaaat cagcccgagg ctacgaatat
    15781 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    15841 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    15901 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggcacagc
    15961 agcacgaaag agaagcaaaa gtcagctacc tggtacacta ggcagcgggt cagcaaaggg
    16021 gggcaaatgc cccttttttc atgtcagata atcagatcat tacctcaata aactaataat
    16081 cacatcgcgg ttacaaccag tttgatagtg atagctcaaa cccagacgtg gcgctggctg
    16141 caagcaaaat aagccgcaaa aatgcgaccc catgtttact agccgcaaaa atgcggtcct
    16201 atttgccaga accttttatt attcaattgg ttagtgcaag aaaaagcgtg attgctgttc
    16261 aagaagtatc cagaaaggtc atgattttga cgctgaaaaa aaataggtgg gcatttttgc
    16321 ccactcacta gccgcaaatt tgcccactca ctagccgcat ttttgcggcc caaagtaata
    16381 aatacagaga aataataaat acagatcagc gccaagattt ttcgtctttt cggcggaatc
    16441 atgagactag tgcttacgtc ctgataacct ccagagcaga ccttaacttc tgtgcagcag
    16501 gactacgctc attgccgctc ggctaggtca aatcctcacg ataaagctgt tcggattgtg
    16561 accataatgt taaaggactc tttaaatcat tccttgacga attaactagc tggcttaagc
    16621 ggaatagcat gcaggataaa aacaaaacca gcttagcagt ggggagagct tcgcttcgct
    16681 cgtctcctgg ggccaatgct caatgaaacg acgtaaggca cggagcgctc accataggta
    16741 gacacataaa catctgagag cgcaccttct atcgcgctac gcgctcaata aaaaccaacg
    16801 acaccgccac actgcaagac caatggtttt tatcgcgtat acagaccaga caggaaacag
    16861 cagcaaagca gcgtatagcc aggtgcagac agcacactca cacgacgcac agcaggctta
    16921 agtgatcact caggaaactt tatccccgcc gccgatcacc gcgaactatc atgaccgcgc
    16981 caaattcgaa aggaatggcc catagaacgc gatgaacaca cataagcgct acacttactt
    17041 aacgtcgcca cagagcgatt gagagcgctt aaaatcgatg cgatttcatt aaatttttct
    17101 cactcgcctg aatggtggcg atcaggactg atcgcaacat tgccccgcct cgatattgag
    17161 cgcataatag atacatacat taagcggaaa agagataagt ttattgtgaa aatcaggggg
    17221 ttacatatca tgcgcatatc agaacaacaa cggcagatcc tcgactatct ggcaaaaaac
    17281 gaatcaatca ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt tacccgcgtc
    17341 caggttgacg ccgcccgccg ctcactcatg acgatggtta gtaatgggct aatcaggaaa
    17401 caaggccgcg ctttcgtagc tgaaagacat atcaatcacg cttatagcca ggacgttgaa
    17461 gaattgcgcc gccagatcct ttgggtattc agcaaacagg gtcaggagta tttcctttct
    17521 gaacacccaa atttcgccgc caaagagtta acagtaagcg gattgtcgtg cgcactggat
    17581 tatcttgtcg gtgaaggttg cgttacttac aagcagctat ggcacgccct gaatacgctt
    17641 gtagaagatg gtttactcat tgccacgcgt gagaacatcg aatactggat aatgccgcgt
    17701 tcaggtcgcc gcaatcatgc acggtatatt tcaggcaaca gatacagaac gccggacttt
    17761 aatccgacac aggaagatcg ggaccgtgcc gaaatgatgt gcagggaaca ggagaaaaga
    17821 attatggata agttattttc gatgcagtga acacaagcgg gcgcaatgcc cgtttttatt
    17881 gccttattcc acgcctgacg aatcaatttg atcacacctg agaaaatgca tagccagaag
    17941 ttaaggccgt tatattgcga ttgggagcgt tttaaacggt atcaatttaa ttaattctga
    18001 tgtggtgatt tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag acatatcgtt
    18061 gtagttatta acagatcact ttgaggtcga gggcggctaa ccctcgctaa tattatcccg
    18121 ctattacctt gcgggtgcaa ataatctttt attgctttga tgatttaata aatagcactt
    18181 tttgctaaca aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa aaataaggtt
    18241 tataatatta agtatcacat gggtaatggg ttcagggaat cggaatccac cgcctcacgg
    18301 aaattcaaaa tttgcttctt ctttcctccg tgggcggctt ctttttcgta tcgaggtatt
    18361 tatgaaagca ataacattag aacagttcgc agcaatggcg aatgtatcga ccagaacgct
    18421 gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg ccatttaccc cacgcggtac
    18481 tcgccaatta ttctgggagc acgaatgcaa agaatggttg tacagaacag aacgataata
    18541 atcacgcatc acattttgag gtaaacatca tgaccaatat cgaaagatta gaacgctgtg
    18601 ccgatattat gcgacgcaga tggatttatg accctgaagc gggtttattg tttagccgtg
    18661 aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg gcatttggtt gtaacggtac
    18721 atgaaggtaa attcaaagtg acactcacct atcagaaagc ctgctatgta tacacatacg
    18781 gcgcttatga cgagacatta tatgaagtgc accacgttaa tttgaacaaa caggataacc
    18841 gcatcaagaa tattcgcctg atgccgaaag aaaagcaccg ccggatacat aaaaacgtcc
    18901 gcaaacttat tcggctgggc cacacacaat ggcttcaacc gcaattaatt gaacaatcac
    18961 tataaacaca ggagtaaata ccatgcattc attagacagt tattttcagc gcacgacagc
    19021 ccctaaatca gccgcccagg aacgccgtga ggaattccat gagaaagtga tgcgttctgc
    19081 tgattatatt gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg aagtagctga
    19141 taaacttcag tctgaaatgc cggaagatat ggaaggcact gcaaagcggc gactcatttg
    19201 cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga aaaaaggggg
    19261 gaaagataat ggacgcattt gaggtactgg caaataaagg cgctgaactg gtagcacagc
    19321 gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa taaacagatt aaagcgatcc
    19381 gtatggccca gtttaaatta atcagtaatg aagttatcga aacaattaaa ccgcagatcc
    19441 cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt gccgggctaa
    19501 aaacgaacgc agagaaaatg gagatgtaca aacagagcgg tgctccgaat cccgatgagt
    19561 acttctcacc ggatgttgag tttatggcgc aggttgagga acgtctgggt agctgtctca
    19621 ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt gacctgaact
    19681 cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg gagccagaac
    19741 cagaaatggc caccgatcct gaagctgagc aacggaaacg cgtattcaat gcgataggga
    19801 ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta acaatgtgac
    19861 caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg ttcgggaact
    19921 atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg cagggcatcg
    19981 ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc aaacaggcca
    20041 tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct tggaactatc aataaaatca
    20101 tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc atcaggcgtt
    20161 taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc gatattaatg
    20221 atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc tgaacatgaa
    20281 gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag cgaaacaact
    20341 acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa ccaaatacat
    20401 cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg actggctgga
    20461 ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct gaggtgagca
    20521 tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc gttgaaatca
    20581 caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca ggttggcatt
    20641 gaaacacatc taccatgacc ataataaagt aattttttga tatgtttatg gttttatatt
    20701 aattaaaata tatctaaatt tgatagtttt aaaattgcaa aatccagatt gtgtttgtaa
    20761 tgtttaatct gattgtattg agtggcgttt attatgaaaa ataaatcagg taattcttgt
    20821 tatagagaag cagtcgatat tcatagctca cttgagcttt catttatatt gcataaatat
    20881 catttgcgtg tagatgagaa gaaatctcga aatgtattca gcgccatacg tgatgagctc
    20941 atccttgatg gggattcacg acaaaactta gctactttct gtcaaacatg gcttgatgat
    21001 gaaatccgtg agttgcttaa tttagctatt gataaaaaca tgatttataa agatgaatat
    21061 ccacaaatgg cggaacctga aggtcgctgt gtgcgtatgc tttccgattc gtggagtttc
    21121 cccgattcgc gacacacctt aggttgctca accattggtt catctgaagc tgctatgctc
    21181 ggccgcttgg cgttaaaatg gcagtggtgt aaaaaacgcg aagtacaagg taaatcaacc
    21241 gaacctgatt tgcgatcctg tgcaaatatg ctggcataaa ttctgccgtt actgtgatgt
    21301 gaaattgtgt gaaattccac ttgatgatca tcacctgata atgaatgcta aagaggtttt
    21361 gaagcatgtc gatgaaaaca ctattggtgt cgttacgact ttaggtatta cttatttttg
    21421 taaagcagat tgcgttagtg tttttaagat aagtgttaaa gttaatatta gtttttatat
    21481 ttacatcgta attatatgtt tctattttaa aaatgttcga gagtataaaa atgtctaatg
    21541 ctaatatcgc acccaaaagt aagaaaatga cagtggggac actcgctatc atgaatataa
    21601 ctgctgtagt aagtctacga tttatacctt ctgaagcaga atacggctta ggtgccattt
    21661 tttactatac ctttgctgca attatgtttt tagtacctgt tgcctttgtt gcggctgagc
    21721 tagctacaac atatccacaa aaaggggggg catttcgatg gataggagaa gcatttgggg
    21781 ctcgctgggg ttttgtagga atgcttatga cctggctaca agtcatcccc tactttccaa
    21841 cagtactgac atttggtgca gtctcggttg catttattga tgttgatatg ggggtggcag
    21901 aatcaattgc cagtaataaa tggtatattt tatttttcgt actatttgtt tactggggag
    21961 ctatttgtgt tgcattacgt ggcgttggta ttttctcgca ggtatctaag tggtgcggta
    22021 ttatagggac aattattcca gctattatcc ttgttattct tggtttctcc tatctgtggt
    22081 tcagcggtaa accagcggtg atcccattag gttggggtga tcttttccct aatatagcta
    22141 acttccagaa tattgttctt gccgccagca tatttcttgc ttatgcaggg atggaaatga
    22201 atgcggtcca tgttaacgat ttagataatc caactaaaaa atacccaatt gcaattacca
    22261 tcgcttctat aggtactatt gcaatattcc tgctcagtac tttaggggtc gcctttatta
    22321 ttcctacaga taaaattagt ctaactcaaa gtttgttggt tgcctacgat atgctgttcg
    22381 aatgggctgg agtgccatgg cttggctctg tgatggcatt tatgctagca attggcgtac
    22441 tgggtggggt ggttacttgg attgcagggc ctaatacagg tgtattagcg atcgcgaaag
    22501 ctggttattt acctaagttt ttccaaaaaa ccaatcgtca tggaatgggg caccatctaa
    22561 tgtttgtaca aggtataatt gtctcagttc tttctgttac ttttgtgata atgccttcag
    22621 ttcaagcagc ttttcagatt ttatcacagt taacagttat gctctattta gttatgtata
    22681 tgctcatgtt cgctagtgct atctatctac gacattctca acctgataca aaacgcccat
    22741 atcgagcccc tgctttattt ttctgggcaa cacttggatt cttagggtct tttctggctt
    22801 ttgtattaag ttttattccg cccgagcaaa tcccaactgg tagtccaacc agctatgttc
    22861 tttatttgtt agtattagtt gccctatttt tattaattcc aatattaatt tacgccttcc
    22921 gaaaatctga ctggaaagat ccaaactctg atttcgaacc attttcttgg gaaaaaagaa
    22981 ctagtaatta aattttccat aagctacaaa agatgaggtt aaatatgttt agtgctgaat
    23041 ctccaattgg tgtaatagac tcaggtgtcg gtggattgac atcggtaaag gaaataatta
    23101 atttgcttcc tggtgaagat attatctatt gtggagataa tatgaatgca ccttatggca
    23161 accgttctgc cgatgatatc atcagtttga ctaaaaaaat gctgactttt ttacagtcaa
    23221 ggaatgtcaa actagttgct gtcgcatgta atactatatc ctcaactttg gaatccgaag
    23281 aatacagcgg atatgctaag agctttccgt ttcctatctt atccatcatt gaacctgcag
    23341 tagaagatgt gattcgccag caatataaaa atgttggtat tatcgctacc gagtttacta
    23401 ttaaaacggg gtgtcacaaa gagctaataa aaaaattgaa ttcaacaata aatgtatttg
    23461 gagaaccaag caaaaatctt gcaatgttaa ttgaggaagg taatcttaat gctcctgcaa
    23521 tcttgaatga catcaaaaaa catgttaacc atttaatttc cctccatcca gtaaatgaaa
    23581 ttattctcgg ctgtactcac tatccaattg ttcagaattt ttttgaagca gttgcaccgg
    23641 atataaaatt tattaatcct gcacatgatc aggctatctc tattaaaaat cacttgggac
    23701 agctaaattt attaaactcc tcaaatatag gaactttgca aataaatact agtggaagta
    23761 tggagatcta caaaacggta ctaggggaat tgagcattac gaaacctcat acattcagta
    23821 tcaggcagtt ttaaatttat ccggtacacc gtaaaatccc gtccttctga actgcacccc
    23881 aaaagttgga caccaactga gtaaggtgca ttttatggac aaacccagtt acatactggg
    23941 tagtgcatcc aattagtaga acatgtgttt ttcgataaac gctccgatca ctttttcgtg
    24001 gatctcaact gagcgtgaga agcagattgt tttacgaccc aaccgcttaa tgcgggtgcg
    24061 tagcgtcaga ttattacgct caatgcgttg ggtgaatatt ttgccggtca gatgcttatt
    24121 cttcggcacc tcccggccat agctgcccca gtcatcgctg gtcagcatgc cgatgttgaa
    24181 gggtgtaagc agtgccagta gctcccggca cgtttgatcg gttcggggac caaaagtgta
    24241 ggccagtaca ccgcctgttt tggtgttgta cgcgtacccg agccaatgtt gccgggcttt
    24301 actgccaacg tagctccatt gctcatcaag ctcgcagata aacgccacat cagcatgggc
    24361 aacaggcgaa gaagttattc gctttggcgc gagtttttta aagtccggat gacggtgtta
    24421 ctaccaattt tcagtgtcct ggcggtatcg cgaaccccgg caccattaaa ggccatttca
    24481 gttatcagct ctttcatacc cggcttacgt gcttgataag tataagtgaa ctgaaacaca
    24541 cggtggcagt cacggcagcg aagtctgtca cggcctttag ggttctgacc atggcggtaa
    24601 acctgagctg actgacaacg gggacaatga atattaacgc tggtcatgag ataacctcaa
    24661 aagcccgtat tatacatcag attcaactaa ttagaggcat cacccatcca gcattataaa
    24721 acatactaac aataactaaa ttataataat cacaacttac aatcaaataa taaatgcata
    24781 agattaacaa tgattttttc gggttatatt tatctaaaaa agcagaatat tatgtaactt
    24841 attattttct aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg taccacccga
    24901 agaacatgcg aagatttaag tattctttct tttttggttt ttatttttca ttttctcttt
    24961 tcttctcttt ccggttctgt tctggttttg gttttttcct ttttattttt attctttctt
    25021 cagggttatg ctaaaacacc aaaacatttt tcaccacaaa agcatccaat catcttgcca
    25081 gaccaatcac cttaaccgga tacactgccg gatataatcc cactaacacc cagacaccaa
    25141 gcaccatacc tgataactac cgtattcagg aaggaaatgc gagtgatttc accgtcaccg
    25201 gcacaaccga tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa
    25261 tcacttcgtc aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca
    25321 gagctttacc aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg
    25381 cgacgaaatc gaccagcgcg ttgatgaccg tcagaaccgc ggcgtaacgc acaggcagcg
    25441 aggcgggaag cgccagcacg tacacgtcag ttttgggatc aagatccagc tgaagcagcg
    25501 cctgttgcca ggcactcacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc
    25561 gcagcggcag ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa agggtatgtg
    25621 cacccgcgcc aatcaccgtg gagcgtacgg tttgcgccgg aaactgcaca ttcatctcac
    25681 gcaggcgcgg atggtcatgc agcgccgttg ccagaagtgg gccaatatcg gcaaaacaga
    25741 acgggttggc gggctggtgg cgataacatt cgcccacgcc tccagaaagc gtaatgattt
    25801 cgggcgtaac acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaaa
    25861 gcgttccgtc aatcacttcg acaatcagcg ccgccatccg ccgggtaacc tgcaccagct
    25921 gcgcgccggt cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc acaatcatct
    25981 gccccggttt atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccac
    26041 cgacgttgag acaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg
    26101 tgccaccgcc gatatcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgcg
    26161 ccccggctcc gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg gcgacgacaa
    26221 aatcgcccag cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt
    26281 cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg
    26341 ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt
    26401 cggtgatggt aaaacccagt agatccgcca cttcacgggc ttccggggta ataatgctgg
    26461 cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg
    26521 ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc
    26581 agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc
    26641 gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg
    26701 atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt
    26761 tccgggtcat ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt
    26821 aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta
    26881 tcattaaatt ccacggccag tgtttttctg gcgagggtat aattcccctg caacgcatta
    26941 aataacgttg tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc
    27001 tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg
    27061 acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg
    27121 ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccggcg
    27181 atcatcgccg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg
    27241 ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt
    27301 ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa
    27361 actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt
    27421 taaaaggtga ggtagatcaa taagaatgga gagatattta ctgttatgaa taaactttca
    27481 tatgcgacgc atgaatataa ataaaataat atcgagatat tttatatact gattttttta
    27541 ttattcgcaa actcagtaat aagctttgct aatatatttc aggaaaaccg cttaccctga
    27601 atattcaggg taagcgtgag agttaagaat gaattacagc ggttgggttt gcgcttcaac
    27661 cacggccagc gccaccatat tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg
    27721 aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg agctggaaac
    27781 gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattggc
    27841 agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc
    27901 atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga ccagttccag
    27961 cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa
    28021 cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca tcaaggtgat
    28081 ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt
    28141 accactcggc agcagcagcg cattcattgc acctgcggtg tgaacgccat cgcgataacc
    28201 aaagacattt ttcaccacac taaaatgttc atgataatcg cccaccgtac cgcagatcat
    28261 tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag
    28321 cgcacgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga agtattcggt
    28381 ccagtactct ttaaagcgcg gatcggattc gttattgacg atttcaaaat caacgcccgc
    28441 tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccgataag
    28501 gatcggtttc gccagtccca gcgttaccag ttcctgagtc gcatgcagaa cgcgcgcctc
    28561 ttccccttcc ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt
    28621 cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc
    28681 agcaatcgga cgcgtcgcca cgcccgactc catcgccgct tttgcgaccg caggagcgat
    28741 cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag
    28801 atcctgaacg ccatacgctg aagccaccac ttcactctgt tctgcatggg ccagttctgc
    28861 aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc caacgtccag
    28921 cgcgccacgg aagatgaacg ggaagcaaag aacgttgttc acctggttcg ggtagtcgga
    28981 acggccggta cagatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc
    29041 cggttccggg ttcgccagcg ccaggatcat tggtgcccga gccattttct tcaccatttc
    29101 ctgggtcagc actttcgggc cggaacagcc caggaaaata tccgcacctt caatcacatc
    29161 atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt ccgccatatt
    29221 tggctcacgg ccctgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg
    29281 cagccccagc gctaccagca ggttcataca ggcgattgct gcggcacccg cgccggaaac
    29341 caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga ggatggcggc
    29401 agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc
    29461 gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgatgcc
    29521 gccgaaggtc ggttcgagcg cggcgacgac ttcaataaat ttgtccgggt cgagttcatc
    29581 aacttcaatg tcaaatacat caatcccagc gaatttctta aatagaacgc ccttgccttc
    29641 catcaccggt ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt
    29701 agagatcacc gcaaccaggt taccgcgcgc ggtatatttg taggctttta gcgggtcttt
    29761 ttcgatttca agacaaggtg cggcaacgcc tctcttttga taaagaaaag agtattgaga
    29821 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattttga
    29881 tcaaactggc ttcgacctgg gaaggaattc gcgcggccga agagctggaa aaagaaggta
    29941 ttaactgcaa cctgacgctg ctgttttctt ttacccaggc gcgggcctgt gcggaagcag
    30001 gcgtttttct gatttcgccg tttgtagggc gtatttatga ctggtatcag gcacgcaagc
    30061 cgatggaccc gtatgtggtg gaagaagatc cgggcgttaa atcggtgcgc aatatctacg
    30121 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
    30181 aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg
    30241 agctgcagga aaaagtttct ccagtggtac gtaaattaat cccgccttct cagacgttcc
    30301 cacgcccagc acccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg
    30361 cggtagaaaa actgtctgaa ggcattcgtc tgttcgctgt tgatcaacgc aaactggaag
    30421 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
    30481 gccaatgcga ttcgcgcact cagtatggat gctgtacaaa aagccaattc tggtcatccc
    30541 ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat
    30601 aatcctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
    30661 tcgatgctgc tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg
    30721 aagaacttcc gtcagttgca ttcgaaaacc ccaggccacc cggagattgg ctatacgcca
    30781 ggcgttgaaa ccaccaccgg cccgcttgga caaggtttgg cgaacgccgt cgggctggcg
    30841 atagcagagc gtacactggc ggcgcagttt aaccagccag accatgagat cgtcgatcac
    30901 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt
    30961 tcgctggcag gcacgctggg actaggcaag ctgattggtt tttacgatca caacggtatt
    31021 tccatcgacg gtgaaacaga aggctggttt accgacgata cggcaaaacg ttttgaagcc
    31081 tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg
    31141 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
    31201 ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc actgggggaa
    31261 gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
    31321 aaagagattt atcacgcctg ggatgcccgt gaaaaaggcg aaaaagcgca gcagagctgg
    31381 aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttccccga
    31441 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag
    31501 ttacaggcaa atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct
    31561 tacgggccga tgctgcctga gttgctcggc ggttcggcgg atctggctcc cagcaacctg
    31621 accatctgga aaggttctgt ttcgctgaag gaagatccgg cgggcaacta cattcactac
    31681 ggggtgcgtg aatttggcat gaccgctatc gccaacggca tcgcgcacca cggcggcttt
    31741 gtgccgtata ccgcgacgtt cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg
    31801 gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat tggcctgggc
    31861 gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat
    31921 ttcagcacct ggcgaccgtg cgatcagatg gaagcggcgg tgggctggaa gctggcggtt
    31981 gagcgccaca acggaccgac ggcactgatc ctctcaaggc agaatctggc ccaggtggaa
    32041 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc
    32101 ggtaagccag atattattct gattgccacc ggttcagaga tggaaattac cctgcaagcg
    32161 gcagagaaat tagcaggaga aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat
    32221 attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct
    32281 cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg
    32341 gcaattgtcg ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc
    32401 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt
    32461 gcctgatggt gatcgccgga tgctgtatgt tcccctcacc ctaaccctct ccccagaagg
    32521 gcgaggggac ggtccgtgcg ctttttaggg ccgcatctgc gattttgttt aacgggtgat
    32581 ccacaacgtg ggcctggcgt ctggcccatg ccagttatcg caggtaggtt ctgcggcgta
    32641 acgcaccagg cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct
    32701 taatccgcgc cctttcgcta aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt
    32761 catcagttcc agttcattcg tctcctggcc gttgttgaac ggcaaacgca accgaaccgg
    32821 gcgcgaggct agcggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc
    32881 ccctgcctca cagctaatca tcatcagtgc tttatcatcg gtcagcgcag tgacattcac
    32941 ctcacgacgt aacggatcaa gcgagcagcg cagaccattc atccgccagt ttccataatc
    33001 cagcaaatcg ttacgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac
    33061 ctctttcagc gcaggcgcgg gcggtacgct gagaggcggt tcgtcccctt tcttgatcca
    33121 cgcggtttca ctgccaacgc gcttttgctg agcatcaata aacaacaacg ccgcttttaa
    33181 gccgctcaga gaaatggtct gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat
    33241 tatctgcaaa aacgcggtga tggttgccgt atcatcggtt actaataacc atggtgaaat
    33301 ccgccacttg tcaccactta atgccaacgg ctcaccatct aatagcagcc gtggcgctat
    33361 ctccccttca gacgcatccg gcgacttcaa cccaccgcgc tcaatacgta aaacggcatc
    33421 ggtatgcgcc ccagcgctgc ggctcagggt catcaccagt ccattatgat cgcccgtatt
    33481 gcgcgccaca caaaaatttt gattattaca ggtgacctgc cagtcggaaa acgcccgttg
    33541 cgctggtgcc gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat
    33601 gcgatagcgc atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt
    33661 cgttgcgcag gtagcgcggc tcaatcggat ttgtcggatt gagccaataa taaacaacga
    33721 aatattattg ctatttttca gtccattaaa tgtgacattt gcaaatagtt aagcaataac
    33781 accgtcttac cgtcacgtat ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc
    33841 agctcgagca cttcaatatc ttcatcttca acaccgccac cggcgttagc gcgctgattg
    33901 tcactgtatt cggcgataaa aaagtggatt agctcagtca caccgccagg cgacatatac
    33961 agttcaaata atttgcgcac ttcgcccact tcatagcccg tctcttcaat cgcttcttta
    34021 cgaatgcaca cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc
    34081 ccgctttcat tgccattaac ccaggtagcg acacggaact gacgaatcag aaccacgctc
    34141 tttttcttcg cgttgtacag gaggatcgtc gcaccattac cgcgatcgta aacttcacgt
    34201 ttatggcgga taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg
    34261 aaatagttat cggagagaat tttgtcttta atgagggtga tttgttgcgt cataccgact
    34321 ccacagcgcg aaatgaacaa ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta
    34381 tcagtgagat ttgactgatt ttacccccag ccagtcaata atcccctgtg ccgcatgacg
    34441 tccttctgcc attgcggtaa ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa
    34501 gattttcgga ttggtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc
    34561 ccatttgtct accgttacac cgtgcgactc cagccacggc atcccgtgtg gattgaagcc
    34621 aaacgccata atcaccgcat cggctggcat gacaaattca ctgccctcaa tcggtacagg
    34681 tcgccgacgc ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac gaattccgca
    34741 aacgtgacct tgctcattca gctcaagcgc caccggctgg acgttaaatt cgaagttggc
    34801 cccctcttcg cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg
    34861 acgataagcg caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca cacagtccat
    34921 cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg gcggtattga taaacggctc
    34981 ttccggtagc tcttcgagcc ccatcacctg tttagtgttg gcaatgagga acggcagcgc
    35041 gtcataaacg cccggcgcat cttcattggg taaacccgct ttcatggaac ggtaagtgcc
    35101 tacgccaacg aagaccgcgt cgtattgttc caaaagcaaa tccaaagaga catctttacc
    35161 cacttcacaa ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc
    35221 cagcagggat ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc
    35281 tggatggcga tcgtacaccg tcaccgccac gccattgcgg gtcagaacat ccgcacaggc
    35341 cagccctgcc ggacctgccc cgataatcgc cacccgcttg tccactttgg tgacatgact
    35401 taagtcagga cgccaacctt tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc
    35461 gatagttacc gcgccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg
    35521 cggacaaacg cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa gctcgacggc
    35581 ggcgtcgatg tttccggctt tcaccagttc aatccactgc ggaatatggt tatgcagcgg
    35641 gcaggtccat tcacaaacgc tgtgctcgcc gaacttaagg cagcgcgagg cttcccgttg
    35701 tgcctggtcg gcgcgaaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat
    35761 cgccagttta tccggctcgc cacgcgcggg cgttgcctgc atttgttcga ctttactcat
    35821 taccggcatt tcttgtgcag cggtactggc atgccacggt tgatgctcct gacgtgcggt
    35881 gcgcaagcgg cgggatttcg ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc
    35941 gtccgccggg caattctcga cacaggcagg accgttttcg cgccctgcac aaaggtcgca
    36001 tttatgcgcc gtggctttta cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt
    36061 accaaacgga caggccacca cgcaggattt acagccaata cacttttgct gattgacctg
    36121 aatgctgtca tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc
    36181 ttcacaatgg tgacaggtca ctgcactacg ttgctgctga tgtttgataa ccgtaattcg
    36241 gggatgaaaa tggtgttggc tcaggacatg ttgctcatca ttgtgagcca tgacacaggc
    36301 gatttcacaa gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat
    36361 aacgaccttc ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg
    36421 acttacaggg gaaccggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac
    36481 tgtggcagat caaataatcc catcactgac taaattgcgt ttcagcgaac tggaacatta
    36541 atgatttttt gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc
    36601 ccgggccttt ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac
    36661 tctggcgagc agtttgcgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca
    36721 gagttaccgc ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca
    36781 gttgtttcag caggcactgc attcaccggt attaaccaac ctcaacgtct ggtatgtgcc
    36841 agaagcagta aaaactcgct atgcgcatct gaatgccaac tggctggaga tgaataatcg
    36901 gctcagcaag ggcgatttgc cgtggtatca ggccaatatt aataattatg ttaatcagat
    36961 agacctgttc gtgctggctt tacagcacta cgctgaacgc aaaatgctgc tggtggtggc
    37021 gatttccctg gctggcggta tcggtatttt cacgctggtc ttttttactt tgcgccgcat
    37081 acgccatcag gtggttgccc cgctgaatca gctggttacc gccagtcagc gtattgaaca
    37141 cgggcagttc gactcgccgc cgctggatac cagtctgccg aatgagcttg gtctgcttgc
    37201 aaaaaccttt aaccaaatgt cgagcgagct gcataaattg tacctttcgc tggaggcgtc
    37261 agtagaagaa aagacccgcg atctccacga gaccaagcgt cgtctggagg tgttgtatca
    37321 gtgcagtgtt cccaggcgct gaacaccagc cagattgatg tgcattgttt ccgccatatt
    37381 ttgcagattg ttcgcgacaa tgaagcagct gaatatctgg agctgaacgt tggggataac
    37441 tggcggatta gcgaagggaa gccaaaccct gagctgccaa tgcagatttt accggtgaca
    37501 atgcaagaga cggtttacgg cgaactgcac tggcaaaata gtcacgtttc atcatcagaa
    37561 ccgctgctta acagtgtttc atcgatgctg ggacgcggtt tgtactttaa tcaggcgcag
    37621 aagcattttc agcagttatt gttgatggaa gaacgtgcga ccatcgcccg cgaattgcac
    37681 gactcgctgg ctcaggtgct ttcttactta cgtatccagt tgacgttact gaagcgttcg
    37741 ataccggaag ataacgccac cgcacaaagt atcatggccg atttttccca ggcgttgaat
    37801 gatgcttatc ggcagttacg cgagctgttg actactttcc gcctgacgct gcagcaggcg
    37861 gatctcccct ccgcgttgag ggaaatgctg gatacgttac aaaatcaaac cagcgctaaa
    37921 ctgaccctcg actgccgtct gccaaccctg gcactggatg cgcaaatgca ggtgcatttg
    37981 ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc
    38041 gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg tttatatccg tgataacggg
    38101 attggtatcg gtgaaccgaa agaacccgaa ggtcattatg gactgaatat catgcgcgaa
    38161 cgcgcggaac ggctgggcgg gacgctgact ttttcacaac cttccggcgg cggcacgtta
    38221 gtgagtatta gctttcgctc cgcagagggt gaggggaagg ggaacacgcc ggctgaggtg
    38281 taaataatta ccctcacacc ggccctctcc ctttaaaggc gagagggaaa aacgtgccca
    38341 tattgacctg taagcgagtg tgtcagcaat ctcatgataa tttacattaa ctcctttttt
    38401 tctccacgat tggctcgtac cttgccgcta cagtgaagca agtcaagcct acaacaatac
    38461 gcagaaacac gaggtcctct tttaatggcg aatttcttta ttgatcgccc catttttgcc
    38521 tgggtgctga caatcctgtt gtgtctgaca ggtaccctgg cgattttttc attgcccgtt
    38581 gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga ccgctaacta tcccggcgca
    38641 tcggcccaga cgctggaaaa caccgtgacc caggttatcg agcaaaatat gaccggcctc
    38701 gataatctga tgtatatgtc atctcagagc agtggcaccg gtcaggcatc tgtcacttta
    38761 agttttaaag caggcaccga tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa
    38821 tcagccatgc gaaagttacc gcaggcggtg caaaatcagg gcgtgacggt gcgtaaaacc
    38881 ggcgatacca acattctgac cattgccttc gtctctaccg atggctcgat ggataaacag
    38941 gatattgctg attatgttgc cagtaatatt caggacccgt taagccgcgt gaatggcgtc
    39001 ggggatatcg atgcctatgg ttcgcaatat tccatgcgta tctggctgga cccggcgaaa
    39061 ctcaacagtt tccagatgac ggctaaagat gtcactgatg ccattgagtc acagaacgcg
    39121 cagattgcgg ttgggcaact tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc
    39181 accattaacg cccagtcact gctgcaaaca ccagaacagt tccgcgatat caccttgcgg
    39241 gtgaatcagg acggctcaga ggtaaggctg ggcgatgtcg ccaccgtcga aatgggggcg
    39301 gagaaatacg attatcttag ccgcttcaat ggtaagccag cctccgggtt gggggtaaaa
    39361 ctggcctcca gcgcaaacga aatggcgaca gcggagctgg tgctcaatcg tctcgacgag
    39421 ctggcgcagt atttcccgca cggactggaa tacaaggtgg cgtatgaaac cacctcgttt
    39481 gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg aagctatcgc tctggttttc
    39541 ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg
    39601 ccggtggtgt tgatgggaac cttctccgta ctttacgcct tcggttacag cgtcaacacc
    39661 ttaaccatgt tcgcgatggt gctggcgatc ggtctgctgg tggatgacgc catcgtggtg
    39721 gtggaaaacg tcgaacgtat tatgagtgag gaaggactca ctcctcgcga agccacacgt
    39781 aaatcgatgg ggcagatcca gggggcactg gtcgggattg cgatggtgct ttcggcggta
    39841 tttgtaccaa tggccttctt cggcggcacc accggtgcca tctatcgcca gttctctatt
    39901 accattgttg cggcgatggt gctgtcagta ctggtagcga tgctcctcac tccggctctg
    39961 tgtgccacgc tacttaagcc actgaaaaaa ggtgagcatc acgggcaaaa aggttttttt
    40021 gcctggttta accagatgtt taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa
    40081 attctccacc gtagcctgcg ctggattgtg atttatgtcc tgctgcttgg cggcatggtg
    40141 ttcctgttcc tgcgtttgcc gacgtcgttc ttaccgctgg aagaccgggg catgtttact
    40201 acctcggtac aattgcccag cggttcaacc caacaacaga ccctgaaagt cgttgagcaa
    40261 atcgagaaat attacttcac ccatgaaaaa gacaacatca tgtcggtgtt tgccaccgtt
    40321 ggttctggcc ctgggggtaa cgggcaaaac gtggcgagaa tgtttatccg cctgaaagac
    40381 tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca ttatcgagcg tgcaacgaag
    40441 gcgtttaacc aaattaaaga agctcgcgtt atcgccagca gcccaccagc aattagcggt
    40501 cttggtagtt ctgcaggttt tgatatggag ttgcaggacc acgctggagc gggtcacgat
    40561 gcgctgatgg cagcacgtaa tcagttgctg gcgctggcgg cggaaaaccc ggagctaacc
    40621 cgtgtgcgcc ataacggcct cgatgacagt ccgcagttgc agattgatat cgaccagcgt
    40681 aaagctcagg cgctgggcgt tgatatcgac gatattaacg acacactgca aaccgcctgg
    40741 ggttcgagct atgtgaatga ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag
    40801 gcagctgcgc cgtatcgcat gctgccagat gacatcaatc tctggtatgt ccgaaataaa
    40861 gatggcggca tggtgccctt ctctgctttc gcgacctcac gctgggaaac aggctcgccg
    40921 cgtctggaac gctataacgg ttattctgcg gttgagattg ttggggaagc cgcaccgggg
    40981 gtcagtaccg gtacggcgat ggatattatg gaatcgttag tgaagcagct gccaaacggc
    41041 tttggtctgg agtggacggc gatgtcgtat caggagcggc tttccggcgc gcaggctccg
    41101 gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc tggcggcatt gtatgaaagc
    41161 tggtcggtac cgttctcggt aatgctggtc gtgccgctgg gggtaatcgg cgcgctgctg
    41221 gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct gttaacggtc
    41281 attggtttat cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa
    41341 aaaggccatg acctgtttga agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt
    41401 ctgatgacct cgctggcatt tatcttcggc gtattgccaa tggcaaccag cacgggtgcc
    41461 ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat ttcagctact
    41521 attctggcta tttacttcgt gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg
    41581 aagccgcgcc cggaataagc aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac
    41641 tttccgatta agaacctgct cagcgggttc ttgctgttta tactttgtct caggaattac
    41701 ttacgaagca taacttcgat aaagtctttc cagttcccca gttcacgttc aatcataaca
    41761 acctctctta taattatggg tattctacgg aaacaatata ccgtggtgaa gctaatttac
    41821 tcgattgctg cgatgactac ctccggggga caaaccttat gtaaatacta tggtcctgcg
    41881 gtgataattt gtatgtgata cacagcaaca tttcgagata ttcatacggc atctaatact
    41941 gatttaattc tggttaaaat acagacagat aacaagatga atattcttaa tgtttacgtt
    42001 aaaaatgttt aatattattt aatagttgtt aatttgaata cttcgataat gttatatttt
    42061 ctgataatca tttgcaggca aaatgttttc acccttaaat gagtatttat tctcataaat
    42121 cgaaaaagga ttcattatgg ttacacttta cggcatcaaa aattgtgaca ccattaaaaa
    42181 ggctcgccgt tggctggaag ccaataacat cgactatcgt tttcatgatt accgcgtcga
    42241 tgggctggac agcgaattat tgaacggttt tatcaacgaa ttaggctggg aagcgttact
    42301 caacacccgt ggtacaacct ggcgtaaact ggacgaaact acccgcaata aaatcaccga
    42361 tgcggcctct gcggcggcat taatgactga aatgcctgca attatcaaac gtccattgct
    42421 ctgcgcgcct ggtaagccta tgctgctggg tttcagtgat tccagttatc agcaattttt
    42481 ccatgaggtg tagtctatgt cgtgcccggt tattgagctg acacaacagc ttattcgccg
    42541 cccttccctg agtcctgatg acgcaggatg tcaggctttg ttgattgaac gtttgcaggc
    42601 gatcggtttt accgttgaac gcatggattt tgccgatacg cagaattttt gggcatggcg
    42661 tgggcaaggt gaaacgttag cctttgccgg gcataccgac gtggtgccgc ctggcgacgc
    42721 cgatcgttgg atcaatccgc cgtttgaacc caccattcgt gacggcatgt tattcgggcg
    42781 cggtgcggca gatatgaaag gctcgctggc ggcgatggtg gtagcagcag aacgttttgt
    42841 cgcacaacat cccaaccata cggggcgact ggcatttctg atcacttctg atgaagaagc
    42901 cagtgcccac aacggtacgg taaaagtcgt cgaggcgtta atggcacgta atgagcgtct
    42961 cgattactgc ctggtcggcg aaccgtcgag tatcgaagtg gtaggtgatg tggtgaaaaa
    43021 tggtcgtcgc ggatcgttaa cctgcaacct caccattcat ggcgttcagg ggcatgttgc
    43081 ctacccacat ctggctgaca atccggtaca tcgcgcagca cctttcctta atgaattagt
    43141 ggctattgag tgggatcagg gcaatgaatt cttcccggcg accagtatgc agattgccaa
    43201 tattcaggcg ggaacgggca gtaacaatgt tattccgggt gaactgtttg tgcagtttaa
    43261 cttccgcttc agcaccgaac tgactgatga gatgatcaaa gcgcaggtac ttgccctgct
    43321 tgaaaaacat caactgcgct atacggtgga ttggtggctt tccgggcagc catttttgac
    43381 cgcgcgcggt aaactggtgg atgcggtcgt taacgcggtt gagcactata atgaaattaa
    43441 accgcagcta ctgaccacag gcggaacgtc cgacgggcgc tttattgctc gcatgggggc
    43501 gcaggtggtg gaactcgggc cggtcaatgc cactattcat aaaattaatg aatgtgtgaa
    43561 cgctgccgac ctgcagctac ttgcccgtat gtatcaacgt atcatggaac agctcgtcgc
    43621 gtgatgagtg gttctgcaag aggaaataag catggactgg ctggctaaat attggtggat
    43681 tctggtgatt gtctttttgg taggcgtcct gctgaacgtg attaaagatc tcaagcgcgt
    43741 cgaccataag aaatttctcg ccaacaagcc ggagcttccc ccgcatcgtg atttcaacga
    43801 taagtgggac gatgacgacg actggccgaa aaaggatcaa ccgaagaagt aagctgagtg
    43861 ctcaaccgta ggccggataa ggcgttaacg ccgcatccgg caatggtgaa cgatccctga
    43921 tgcgacgctc acgcgtctta tcaggccagg taatctgtct tttacatcat ttcaatcaca
    43981 tcatcatcgc caggcttacc gccacttagc gcttggtcga aataatgttt cggaatggta
    44041 taacgcaagt gatccagggc aaattgcata ctgcgattgt cgatcgcatg gcctaaatcc
    44101 tccacgatat ccagcgtcac gtcaccaccg gcactgatta acgcttcctg cgccgcaact
    44161 gcatgcgcca gatcgataac cggatcttca ccaccatgaa tcaagtgaat tgtcgtggct
    44221 gtagacgcgg tctccggcag gctcgcatag cgtccgttaa aggcaatgac gcgcgaagca
    44281 aggcccggtt cggctttaat gctctccagc gccataatcg cgccttgtga gaaaccaatt
    44341 agcgccgtgg cgttagcccc tactccgctc tgtttctgcc agtagcgtac cgtttcaata
    44401 aacgtcggca tgatggcatc cacacgcgcc tggcgattat cttccgtaat accctgcact
    44461 gaaaaccact gacgccccgc cggattaccg ctcggctccg cgccaccgac actcaccacc
    44521 agcgcatcag gaaacagcgg tgcaaaccag ctgcctattt cccccattgc caccgggtta
    44581 tccccgacac cgtggaaaag cagtaacaac tgttgtgcag gtttatccgg gctttgaaca
    44641 acaaaatggt catgtttcat ggcgaactcc ttaactgatg tcattaattt tacgccgctg
    44701 actcacaatg accatgggag aaaattgaag gagtcagtga aaaaattgcc attgcgttat
    44761 gcgatcgcgc agacgctcag tgcgaacctc atttagtgcg aacagcgcct gcgcggcctc
    44821 ttcccgctgg cgaaccagta acatcttgcg gcctgaaagt ttaagtgtgg tacataactg
    44881 cgcgtcactg gcgtttttct gtaaacgccc acgcagcgcc ggtaatgcca gttcactggt
    44941 ttgtagcaga cgcaataagc aacctaacga tgttaatagc ggacgatgag cgaaagcaaa
    45001 accggccagt tcaagccagt cgtcgtcaga aaggatggca tcgtttagtg gatcaacagg
    45061 aagcatttcg ccattccact gcgcgagagc ttgcgcatcg cgacgtaaac ggtaatgctc
    45121 acgttcagcc agctgtttac ccgcatcact catcggtaac agcgccatcg ccgtatagca
    45181 accgctgctg gcttcccgat gattacccat ccgcaccagc acaaaaccgc agcgttgcca
    45241 gaaacgccat aactccccag tgtaaccaaa actcaccgaa agatagtcga ggtcatgcgt
    45301 atattgcaaa gctccagcaa taagttgtcg ccctgtgcct tcccgctgac gagccggatg
    45361 aactgctatc cggctaaccc gccttccgcg caatgtcgcc gccagtggat tgctgccgtg
    45421 cgccgccagc gactgggcca ccagattacc ccgcgggcga cgaaaacctg cccataccgc
    45481 ctgactgagt tcttgagata atccgccctc atccaccagc cacaacgccc cggcaatctc
    45541 gttttcgcca gccgcctgta aaaaatgttg ccctggtgca tccatcatcc ggcgtaaatc
    45601 cagcggcgag gtccggtagt gcgcaccaga caataactga taaaccttta acggcgtttc
    45661 tggctcgctt cgccataacg tttgttcaaa tgcggaaatg acgatattgc cttgtggtgt
    45721 atgggtgaag ttttcatcgt taaaaaccag tgcctcacta accatttttt ccagcgggca
    45781 tccctgtgcc cagcggatcg gctgttgcag ttcaaaacgg tgtaaatgcg gaaagcgagc
    45841 gcaaaatttc agcaaaaaac cacgtccggt accttcgtag ccctgcaccg tagtggttaa
    45901 caacgttcga ggaaaacgcg ataccagttg atgcaacaac ggcgcaggta tggctgcggc
    45961 ttcgtcgacc accagccagt cggcttgctc atcgctggct aacaaggcat ccggtgcaat
    46021 aaagcgaaac ttctcgcccg caaattgtgc cagtacatcc gttgccgctt ttgcgggcgc
    46081 ggtgacaatc gcactgcccg caatacgaga aatgagttgc cctgccaacg ccgacttacc
    46141 gcgcccacgc gcagccgtta ccgctgccac gcccggcggc atggtcagta gctgctgtaa
    46201 gagttgctgt tgttctggtt gtggtgcgcc ggtcgcgggg tgccagtcag tacggggagt
    46261 aaaatgcgcc aacgagaacg gctggttttg ccgccagagg atagcgtcgt tatccgccgt
    46321 aagtacgcgt ttgagatgct ggacaaaatg cggcgtcgca atagggtcag ggcaatcact
    46381 ccagcgcagc gagtcggcat caggttggtt ttcccactct tcccatacag ggagtaacaa
    46441 aaccagccag cttcccgctt tcaacgttcc gctaagtgct gcaaaggcag cggcatcaaa
    46501 gccgtggcgg gcgtcgaata ccgcatgccg gaactcgcgc ccaagtaaag tttgtagcgc
    46561 cgagggtgaa cagtggtttt cagcatctag ctgcggcgaa atccacagcc agtcgccagg
    46621 taaggcatca cgcaacttaa gagcatgatc aaaacaccaa ccctcttccc cgctcaacac
    46681 cagcaagcgg cagatccctt cacgtttcat ttgcgctgtt aatgtgtaaa gcgcagtcag
    46741 ttcagccatc cctgccccgg aaattaaatg cttttaccaa aagtattgca ttgtgccgga
    46801 tcgccgctgt cgaaaccacg tttaaaccag ctgtagcgtt gctgagaagt gccatgtgtg
    46861 aaactgtctg gtactactcg cccctgactt tgctgttgta aacggtcatc gccgatggcc
    46921 tgcgccgcat tcagcgcctc ttccagatcg ccagtttcca gaacgccttg ctgctgcata
    46981 ctatgccccc agacaccggc aaaacagtcg gcctggagtt ccatacgcac agataagcgg
    47041 tttacttccg cctgcgtcgc gttttgttgc agttgacgaa ctttcggctc gatgcctaac
    47101 agtttctgca catgatgacc gacttcatgg gcgataacgt acccctgggc aaaatcgcca
    47161 tccgcgccaa gtttgtcttt catgtcatca tagaaggaga gatcgatata aactgtgcca
    47221 tccgccgggc aatagaacgg ccccataatg gactggcccg ccccgcagcc ggtgcgcgtc
    47281 attccacggt acatgaccag cttcggttgc tgataggtct tacccatctt ttcgaactgt
    47341 tgtccccagg tgtcttccgt ggttgcctga atcaccgagg tgaattttgc ggcttcgtct
    47401 tcattagggc taattgaccg cgttgattgt tgttgagaaa ccggctgccc ggtcatcaac
    47461 ccggttaaat caacgccata gtagcctgca accagcacga ctatcagtaa aatcagcccg
    47521 cctttaccgc tcggcagacg gaaaccggga ccgcccattg acggaccgcc agagctgttg
    47581 cgcctgtctt caacattgtc actttcacgt cgcccttgcc aacgcataga tacctcaaca
    47641 atatattcat tatagaaatg atcgtaggtg gttcagagga agattaccac aggaaagcgg
    47701 gatgaaatgg cggaggaatg agcgttcaat gcatatattc cgagagcggt tgtcttgccg
    47761 tgccagctgc acggcaagat gatgaacaga aaaatcagtc cagctgtaca cccaggcggc
    47821 gggcgacggc ttcataggct tcgatcaggc caccgaggct ctggcggaaa cggtctttgt
    47881 ccattttctc cagtgtttct ttgtcccaca ggcggctgcc gtccggggag aactcatcgc
    47941 ccagtaccac ttcacctttg tacagaccaa actccagctt gaagtcgacc agaatcagac
    48001 cagcatcatc gaacagtttt ttcagcacgt cgttcgcttt gtaggtcagc tctttcatac
    48061 gcgccaggtt ctctttgctc acccagccaa aggtttcgca gtaagattcg ttgaccatcg
    48121 gatcgtgcat ggcatcgttt ttcaggaaca gatcgaacag cggcgggttc agctcaatac
    48181 cttcttcgat tccaagacgt ttcactagag agccagcagc acggttacgc acgacacact
    48241 caaccggcac catatccagc tttttcacca gacattcggt atcggagagc agacgctcca
    48301 tttgagtcgg gatacccgct tcagccagtt tgctcataat gaagtagttg aacttgttgt
    48361 tcaccatacc tttgcgatca aactgctcaa tgcgcgcgcc atcccctgct gacgtatcat
    48421 tgcggaattc gagcaccaac aggtccgggt tttccgtgct gtatacggtt ttcgctttac
    48481 cacgatacaa ctcagcttgc ttttgcatct ttatcactcc tgggtgtgaa ttaacgtttt
    48541 aaaatctttt gctgtctggt gtgccggatg ttttgtcgga tgcggcgtga acgccttatc
    48601 cgaactacgt ccgacaaaca aatttcgtgc gaattaccgc taaaatcgcg ttttcctgcc
    48661 gacgcacacg tttgcgtatc atatcagaaa aaagggccgg atgattccag ccctgtattt
    48721 ttacttgcta aacgcagcct ggaagacagc taccagcgcg tcgttctgac tctgagtcag
    48781 agtatgacct ttcggatcga tgaactgtag gctgctgcgg ttatctaaat cgccaacctg
    48841 cagtttatag tcaccggatg ccaggcctgg atcgctcgcg cccagttcct gccagtcgct
    48901 gtcagacagc ggcttataag ttacggccat gttgccctgc gaacgggtgc tgtcggtcac
    48961 tttcatgccc actttttcca gcgccgctgg cagacgttgc caaaccacat tgaacggccc
    49021 gcgtacgacc agcattggta aaccggtgtc atcagctgca ctttgtacgt ccatagtggt
    49081 ggaggcacga ttttgcgcag cgttcgcggc gtcagtggca gatttatcca gaccggcgga
    49141 aataacgttc atcatctccg tgctgtaacg ctgcatggaa gccgcgtctg caaccggttt
    49201 gcccgcctgt tccaggttca gcagtttaac cgtaaccgcc tgctgataac cctgcggctt
    49261 aacagagatt tgataacgac cacgatactg ctcgtcttcg tccagacggt tccattgtac
    49321 ccaatcggtg gtcagtgtct gaccagcatc atcacgttgg gtgatggtgt agtttttcgc
    49381 ctgcagcacg ctaaccacct gcggccacag agtattgcca cgaccatttt ccaccagcaa
    49441 tgaagcggta tcgcccgtga actgggtacg cgcgccagaa accagtgcca acggctgggc
    49501 tggtggacga atgtccagcg ccttaccgac agcaccacta ccgttggtca ccgggattgc
    49561 ataatcaccg gaggtcaccg gcaaaatcat tccagccggg gcatgaagct ccgcaagcgg
    49621 tgccgcttcc aggtaggctt catcaccact gacctgacgc ttatagcgtg agtcagaact
    49681 acaggcagcg agtaataaaa caagcgaaac acccgcaacc tttgccaggc gcgacttttg
    49741 aacagagtaa gccatcaaat ctccctaaac tttacagcaa accggcatgc ttaagcgccg
    49801 ctctgaccgt ctcacgacca ctgtcggtga ttggtgtcat tggcaggcgc agcgtatcgg
    49861 tcgccacaag acccagttcc ttacatgccc atttcaccgg gattggattg ggttcgacaa
    49921 atagtttgtt gtgtaatggc atcagacgct gattaataac gcgtgcctcg gcaaaatgcc
    49981 cttctgctgc cagtttgcac atctgggcca tatcacgcgc tgcgacgtta gccgtaacgg
    50041 aaataactcc atgaccgccc aattgcatga agtccagcgc gctcgcatca tcgccgctca
    50101 gcagaacaaa atcatctgaa accagctctt tgatctggtt tacacgcgtt aagttccctg
    50161 ttgcctcttt gattccgata atatttttta ctttcgccag acggcccacc gtttccggga
    50221 gcagatcgca gccagtacgg gacggcacat tatacagaat ttgcggcagg tcagtatgct
    50281 cagcgatggc tttgaaatgc tgatacaaac cttcttgcga cggacgattg tagtaagggg
    50341 ttaccgtcag gcagccgacg ataccactgt cattgaagcg ctgcgtcagg ctaatggctt
    50401 ccgcagtagc gttagcgccg gtcccggcaa ttaccggaat gcgcccatca gccagatcca
    50461 gcgtcatcat caccacatca gcatgttcgt catgatttaa ggtagcggac tcgccagtgg
    50521 tgccaacaga aacgatcgcc gaagtaccgc tggcgacatg ataatcaatc agttttttca
    50581 agctagcccg acagacatta cctttttcat ccatcggagt aacaatcgcg acaatacttc
    50641 ccgtgaacat gggccatcct ctgtgcaaac aagtgtctca atggtacgtt tggtatggca
    50701 ttaaaagcaa gcagacagaa ccgttctgat tgttgtatgc atgatttttt tatgctttcc
    50761 ttaagaacaa ctcacccctt aaaggaataa ccagtttgac actgtcatcg caacattatc
    50821 tggtgatcac tgcgttgggt gccgatcgcc ctggaattgt gaacaccatc acccgtcatg
    50881 tcagtagttg cggctgtaat attgaagaca gtcgcctggc gatgctggga gaagagttca
    50941 cgtttatcat gctgctttcc ggttcatgga atgccatcac tctgattgaa tcaacgttac
    51001 cgttgaaagg tgccgaactg gatcttttaa tcgtgatgaa gcgcacgacg gcgcgtccgc
    51061 gtccgccaat gccagcatct gtctgggttc aggtcgatgt tgcagactcc ccgcatttaa
    51121 ttgaacgctt cacagcactt ttcgacgcgc atcatatgaa cattgcggag ctggtgtcgc
    51181 gcacacaacc tgctgaaaat gaacgggctg cgcagttgca tattcagata accgcccaca
    51241 gccccgcatc tgcggacgca gcaaatattg agcaagcgtt caaagcccta tgtacagaac
    51301 tcaatgcaca aggcagtatt aacgtcgtca attattccca acatgatgaa caggatggag
    51361 ttaagtaatg aatccactga aagccggtga tatcgcaccg aaatttagct tgccggatca
    51421 agacggagaa caagttaatt tgaccgactt ccagggacag cgtgttctgg tttatttcta
    51481 cccgaaagcc atgacccccg gctgtaccgt acaggcctgc ggcttacgcg ataacatgga
    51541 tgagttgaaa aaagcgggcg ttgatgtgct gggtatcagc accgataaac ccgaaaaact
    51601 ctcccgtttt gcggaaaaag agctgcttaa ctttacgctc ctgtctgatg aggaccacca
    51661 ggtgtgcgaa caattcggcg tctggggtga aaagtccttc atgggcaaaa cctacgatgg
    51721 tattcatcgc atcagcttcc tgattgacgc tgatggcaaa atcgaacatg tctttgacga
    51781 tttcaaaacc agcaatcacc acgacgttgt gctgaactgg ctgaaagaac acgcctgatt
    51841 actttgctcc attccgtgct ggctgcgctt gcggccagca tacctcactt ctcgtgatca
    51901 agatcacatt ctcgctttcc cctgcgacac gggtgtcgaa tccatttttt gctgaacgtt
    51961 aatgaccatc atttttgtac cgttcagaat ccagttaata cataacttat tgaatagatt
    52021 gagttaatca gaatggcatc ctttatacaa tatgaaatgc aatgtttcat atcattttca
    52081 aggagccgac atgaaccgct ttgtggtggc cgaaccactg tggtgtacag gatgtaatac
    52141 ctgtctcgct gcctgttcgg acgtgcataa aacgcaaggt ttacagcaac acccgcgcct
    52201 ggccctggcg aagacgtcaa caatcactgc ccctgtcgtg tgtcatcact gtgaggaagc
    52261 cccttgcctg caggtctgcc cggtcaatgc catctctcag agggatgatg cgatccaact
    52321 caacgaaagc ctctgtattg gctgcaagct ttgcgccgtg gtctgcccat ttggcgcaat
    52381 cagcgcttca ggaagccgtc cggtgaatgc ccatgcgcaa tatgtttttc aggctgaagg
    52441 ctcactcaaa gacggcgaag aaaacgtgcc gccccaacat gcgttgctgc gctgggaacc
    52501 gggtgtccag accgtcgcgg tgaaatgcga cctgtgtgat ttcttgccag aaggtccggc
    52561 ctgcgttcgc gcttgcccga atcaggcgtt acggctgatc accgacgata gcctgcaacg
    52621 tcagatgaaa gaaaaacagc gccttgccgc aagctggttt gccaatggcg gggagtctcc
    52681 cctttccctc actcaggagc aacactaatg gatgccctgc aattattaac ctggtcgctg
    52741 atcctctatc tgtttgccag tctggcttcg ctgtttttac tcggtctgga cagactggct
    52801 attaagcttt ccggcatcac atcgctggtg ggcggcgtga ttggcatcat cagcggaatt
    52861 acgcaattac atgctggtgt aactttagtc gcccattttg ccaccccttt tgactttgcc
    52921 gatttaaccc tgcgaatgga tagcctctcg gcatttatgg tgctagttat ctccttgctg
    52981 gtggtggttt gttcgctcta ttcattgact tatatgcgcg aatacgaggg caaaggcgcg
    53041 gcggcgatgg gcttctttat gaatcttttc atcgcatcga tggttgccct gctggtgatg
    53101 gacaacgctt tttggttcat cgtgctgttt gaaatgatgt cgctgtcttc ctggtttctg
    53161 gtcattgcca ggcaggataa aacgtcgatc aacgctggca tgctctactt ttttatcgcc
    53221 cacgccggat cggtgctgat tatgatcgcc ttcttgctga tggggcgcga aagcggcagc
    53281 ctcgattttg ccagtttcgc acgctttcac tttctccggg gctggcgtcg gcggtgtttc
    53341 tgctggcctt tttcggtttt ggcgcgaaag ccgggatgat gccgttgcac agctggttgc
    53401 cgcgcgctca ccctgccgca ccatcgcacg cttcggcgtt gatgtctggc gtaatggtca
    53461 aaatcggtat tttcggcatc ctgaaagtag cgatggatct gctggcgcaa acgggtttgc
    53521 ctctgtggtg gggcattctg gtgatggcga tcggcgcaat ctccgcgctc ctgggcgtgc
    53581 tatatgcgct ggcggaacag gatatcaaac ggctgctggc ctggagtacc gtcgaaaacg
    53641 tcggcattat tttgctggca gtcggtgtgg cgatggacgg tctgtcactg cacgacccgc
    53701 tgctcaccgt ggttggactg ctcggcgcac tgtttcatct gctcaaccat gcgctgttca
    53761 aagggctgct atttctcggc gcggacgcga ttatttcgcg tttgcatacc cacgacatgg
    53821 aaaaaatggg ggcactggcg aaacggatgc cgtggacagc cgcagcatgc ctgattggtt
    53881 gcctcgcgat atcagccatt cctccgctga atggttttat cagcgaatgg tacacctggc
    53941 agtcgctgtt ctcactaagt cgtgtggaag ccgtagcgct acaacttgcg ggtcctattg
    54001 ctatggtaat gctggcagtc actggtgggc tggcagtaat gtgcttcgtc aaaatgtacg
    54061 gtattacttt ctgtggtgcg ccgcgcagta cacacgctga agaggcacag gaagtgccaa
    54121 atacgatgat cgtcgccatg ctactgctcg cggcactctg cgtattaatt gcgcttagtg
    54181 ccagttggct ggcaccgaag ataatgcata ttgcccatgc gtttaccaat acccctcccg
    54241 tcactgtcgc cagcggaata gcacttgtac ccggcacgtt tcatacacgg gtcactcctt
    54301 cattactgtt gctgttacta ctggcgatgc ctttgctgcc aggcctttac tggctgtggt
    54361 gtcgttcgcg ccgcgcagcg tttcgtcgca caggagatgc ctgggcatgc ggctacggct
    54421 gggaaaacgc gatggctccg tcaggcaatg gcgtgatgca gccgctgcgt gtggtcttct
    54481 ctgcgttatt tcgtctacga caacagctcg accctacgct gaggctaaat aaaggtcttg
    54541 cgcacgtcac cgccagggct cagagcacag aacccttctg ggatgagcgg gtgatccgcc
    54601 ccatcgtgag cgccacccaa cggctggtca aagaaataca gcatctgcaa agcggcgact
    54661 ttcgtctcta ttgcctgtat gtggtcgccg cactggttgt gctgctaatc gctattgccg
    54721 tctaaggaaa tcaccatgag acaaactctt tgcgacggat atctggtcat ttttgcgtta
    54781 gcacaggccg tgattctgct gatgctaacc ccacttttta cgggtatttc ccggcagata
    54841 cgcgcgcgta tgcactcccg ccgcgggccg gggatctggc agaattatcg cgatatccac
    54901 aaactgttta aacgccagga agttgcgccg acatcttcag gtctgatgtt ccgcctgatg
    54961 ccgtgggtat taatcagcag catgctggtg ctggcgatgg ccttaccact atttattacc
    55021 gtttcccctt ttgcgggcgg cggcgatctg atcaccctta tctatcttct tgccctgttt
    55081 cgttttttct ttgctctttc cgggctggat accggaagtc cgtttgcggg agtcggtgcc
    55141 agtcgcgagt tgacgctcgg cattctggtc gaaccaatgc ttattctctc actgctggta
    55201 ttggcgctga tagcaggttc cacgcatatc gagatgatca gcaatacgct ggctatgggc
    55261 tggaactcgc cgctaaccac cgtactggcg ttactggcct gtggttttgc ctgcttcatt
    55321 gagatgggaa aaattccctt tgatgttgct gaagcagaac aggaattaca ggaaggcccg
    55381 ctgaccgaat attccggtgc cgggctggcg ctagcgaaat gggggctggg gctgaaacag
    55441 gtcgtgatgg cagcactgtt tgtggccctg tttctgccct ttgggcgcgc gcaagaactt
    55501 tctctcgcct gcctgctgac ttcacttgtc gttacgctgc tcaaggtttt gctgattttt
    55561 gtactggcct caatcgcaga aaacacgctg gcacgcgggc gttttttact cattcaccat
    55621 gtgacctggc ttggcttcag ccttgctgcg cttgcatggg tcttttggtt aaccggtctg
    55681 taaggagcac tgacggaata tggaaaatct tgctctgacg acgttaatgc tgccttttat
    55741 cggcgcactg gtcgtttcgt tttcgccaca acgtcgggcc gccgaatggg gggttttgtt
    55801 cgccgcgctg accacgctgt gcatgttgtc gctgatctcc gcgttttatc aggccgataa
    55861 agttgccgtc acgttgacgt tggtcaacgt gggggatgtg gcattatttg gcctggtcat
    55921 tgatcgcgtg agtacgctga ttctgtttgt ggtggtgttt ctcggtttgc tggtcacgat
    55981 ctactccacg ggttatctga cggataaaaa tcgcgaacac ccgcataacg gcacgaatcg
    56041 ttattacgca tttttgctgg tgtttatcgg cgcgatggcg ggactggtac tctcctcgac
    56101 gctgctcggt cagttgttgt tttttgaaat tacgggcggc tgctcctggg cgttgatcag
    56161 ttattaccag agcgataaag cgcagcgttc agcactaaaa gcgttgctta tcacccatat
    56221 cggttcgttg gggttgtatc ttgccgccgc cacgctgttt ttgcagaccg gaacgtttgt
    56281 gcttagcgcg atgagcgagt tacatggcga cgcacgttat ctggtttatg gcggcatcct
    56341 gtttgccgcg tgggggaaat cggcccagct accgatgcaa gcgtggctac cggacgcaat
    56401 ggaagcgcca acaccgatca gcgcctatct ccacgccgca tcgatggtga aagtgggcgt
    56461 ttacattttt gcccgcgcca ttatcgacgg cggcaatatc ccgcatgtga ttggcggcgt
    56521 tggcatggtc atgacactgg tcaccattct ttatggcttt ctgatgtatt tgccacagca
    56581 ggatatgaag cggttgctgg cctggtcgac catcactcaa cttggctgga tgttcttcgg
    56641 cttgtcgctc tccatcttcg gctcgcggct ggcgctggag ggtagcatcg cctacatcgt
    56701 caaccacgcg ttcgctaaaa gcctgttttt ccttgtagca ggtgcgctga gttacagctg
    56761 cggcacgcgc ttgttgccgc gtctgcgtgg cgtattgcac accctgccgt tgccaggcgt
    56821 ggatttctgc gtggcagcgc tggcaattac cggtgtgccg ccgttcaacg gcttcttcag
    56881 taaattcccg ctgtttgccg ccggttttgc gttgtcagtg gagtactgga tcctgctgcc
    56941 cgccatgatt ctgctgatga ttgaatcggt cgccagtttc gcctggttta ttcgctggtt
    57001 tggtcgcgtc gtgccgggca aaccgagcga ggccgtcgcc gatgccgcac cgctgccagg
    57061 gtcaatgcgc ctggtgttga ttgtactgat tgtgatgtcg ctgatttcca gcgtaatcgc
    57121 cgcgacctgg ttgcagtaag gagatgatga atgaccggtt ctatgatcgt aaataatctg
    57181 gcgggactga tgatgctgac atcgctgttt gtgattagcg tcaaaagcta tcgcctgtca
    57241 tgcggatttt acgcctgcca gtcgctggtg ctggtgtcta ttttcgccac tctctcgtgc
    57301 ctgttcgccg cagagcaact gctgatctgg tccgccagcg cctttatcac caaagtgctg
    57361 ctggtaccgt taatcatgac ttacgctgca cgaaatattc cccagaacat cccggaaaaa
    57421 gcgttattcg gtccggcaat gatggcactg ctcgcggcgt taattgtcct gctttgcgca
    57481 tttgtcgttc agcccgtgaa gctaccgatg gctaccgggc tgaaaccggc gctggcggta
    57541 gcgttaggtc attttctgct tggcctgctg tgcattgtca gccaacgcaa tatcctgcgg
    57601 caaatttttg gttactgcct gatggaaaac ggctcccatc tggtgctggc gcttcttgcc
    57661 tggcgagcac cggaactggt ggaaataggt atcgctaccg acgccatctt cgccgtcatt
    57721 gtgatggtgt tactggcaag aaaaatatgg cgtacccacg gcacgctgga cgtgaacaac
    57781 ttgaccgcgc tgaagggata atgagatgag ttattctgtg atgttcgctt tactcctgct
    57841 cacgccgctg cttttttcgc tgctctgttt tgcctgccgg aaacgggggc tctctgcgac
    57901 ttgtacggtg actgtattac atagcttagg gatcacactg ctgctgattc tagcactctg
    57961 ggtggtccaa actgccgctg atgcaggaga aatattcgct gcgggactgt ggcttcatat
    58021 tgatggtctg ggcggtttgt tcctcgccat tcttggtgtg attggctttc tcaccggtgt
    58081 ttactcgatt ggctacatgc gtcatgaagt ggcacacggc gagctttcac ccgttacact
    58141 gtgcgattac tacggtttct tccatctgtt tttgttcacc atgctgctgg ttgttaccag
    58201 caataacctg attgtgatgt gggcggcgat cgaagccacc accttaagct cggcgtttct
    58261 ggtaggcatt tacggtcagc gttcatcgct ggaatcagca tggaagtaca tcattatttg
    58321 tactgttggt gtcgcttttg gtctgttcgg taccgtgctg gtatacgcca acgccgccag
    58381 cgttatgccg caggcagaaa tggcgatatt ctggagcgag gttcttaagc aatcgtcctt
    58441 gcttgaccca acattaatgc tgttggcctt tgtgtttgtg ctaattggct ttggtaccaa
    58501 aaccgggcta tttcccatgc acgcctggct gccggatgct cacagtgaag cgccgagtcc
    58561 ggtcagcgcc ctgctctccg ccgtattgct gaactgcgcg ctgttggtgc tgattcgcta
    58621 ttacatcatt atttgccagg ccatcggcag cgatttcccc aaccggttgt tgctcatctt
    58681 cggcatgttg tcggttgccg tggcggcatt tttcattctg gtacagcggg acattaagcg
    58741 tctgctggcg tactccagcg tggagaacat ggggctggtc gcggtggcgc taggcattgg
    58801 cgggccgctg ggaatttttg ccgcgctgct gcacacctta aaccacagtc tggcaaaaac
    58861 gctgctgttc tgcggttccg gcaatgtact gctcaagtac ggcacgcgcg atctcaacgt
    58921 cgtctgtggg atgctcaaaa tcatgccatt taccgccgtg ctgtttggtg gcggtgcgct
    58981 ggcgctggcg ctggcgctgg cagggatgcc gcccttcaac atttttctta gcgaatttat
    59041 gaccgttacc gcaggactgg cacgtaatca cctgctgatt atcgtcctgc tgttattgct
    59101 gttaacgctg gtgctggcgg gcctggtacg gatggctgcg cgggtgttaa tggcgaaacc
    59161 gccgcaggcc gttaaccggg gtgatctcgg ctggttgacc acctcgccaa tggtgattct
    59221 gctggtcatg atgctggcga tgggaacgca tattccacaa cctgtcatca ggatcctggc
    59281 gggcgcttcc actatagtcc tctcagggac gcacgacctg cctgcacaac gtagcacctg
    59341 gcatgatttt ttgccttcag gcaccgcatc tgtttcggag aaacacagtg aacgttaatt
    59401 catcgtcaaa tcgtggcgaa gcgattctcg ccgccctgaa aacgcagttc cccggcgcgg
    59461 tgctggatga agagcgacaa acgcctgaac aggtcaccat tacggtgaaa atcaatctgc
    59521 tgcctgacgt tgtacattat ctttattatc aacatgatgg ctggcttcca gtcctgtttg
    59581 gcaacgacga gcggacactt aacggtcatt acgcggttta ttatgccctt tcaatggaag
    59641 gggccgaaaa atgctggatc gtggtgaagg cactggtcga tgccgacagt cgggagtttc
    59701 cgtcagtcac accgcgcgtc cctgccgcgg tctggggcga gcgagaaatt cgcgatatgt
    59761 acgggctgat tccggttggc ctgccggatc agcgtcgcct ggtgttgccc gatgactggc
    59821 cggaagatat gcatccgctg cgcaaagatg cgatggatta tcgactgcgc cctgaaccga
    59881 cgacagattc cgaaacgtat gcgtttatca atgagggcaa cagcgatgcg cgggtgatcc
    59941 ctgtcggccc gctgcatatc acctcctatg aaccgggtca cttccgcttg tttgtggatg
    60001 gcgagcaaat tgtcgatgct gattaccgcc tgttttatgt ccatcgcggc atggagaaac
    60061 tggcagaaac gcggatgggc tacaacgaag tgaccttctt atcggaccgc gtgtgtggga
    60121 tttgcggttt tgcccacagt gtggcctata ccaactcggt tgaaaatgca ctggggattg
    60181 aggtgccgca acgagcacat actattcgct cgattctgct ggaagtcgag cggctacaca
    60241 gtcatttgct caaccttggc ctctcctgcc attttgttgg ttttgatacc ggctttatgc
    60301 aatttttccg cgtgcgggaa aagtcgatga cgatggcgga attgctgacc gggtcgcgta
    60361 aaacctacgg tctgaatctg attggtggtg ttcgccgcga tattctcaaa gagcaacgtc
    60421 tgcaaacgct gaaactggtg cgcgagatgc gcgccgacgt gtcggagctg gtagagatgc
    60481 tgcttgctac gccgaatatg gaacaacgca ctcagggcat tggcattctc gaccgacaaa
    60541 tcgcccgtga ttatagccct gtagggccgc tgatccgcgc agtggttttg cccgtgattt
    60601 gcgctttgat cacccctacg ccgactacgg caatattcca aaaacactgt ttacctttac
    60661 cggcggcgat gttttctccc gcgtgatggt ccgtgtcaaa gagacgtttg attcgctggc
    60721 aatgctggaa tttgccctcg acaacatgcc ggatacccca ctgctgaccg aaggctttag
    60781 ctataaacct cacgcattcg cgctgggctt tgttgaagcg ccacgcggtg aagacgtgca
    60841 ctggagcatg ctcggtgata accaaaaatt gttccgctgg cgctgtcgtg ccgccaccta
    60901 cgccaactgg ccggtgttgc gttacatgct gcgcggcaat accgtttctg acgcaccgct
    60961 gattatcggt agccttgatc cctgctactc ctgtaccgac cgtgtgacgc tggtagatgt
    61021 gcgcaagcgc cagtcaaaaa ccgtgctgta taaagagatc gaacgctacg gcattgatcg
    61081 taaccgttcg ccgctgaagt aaggacagaa gatgctgaag ttactgaaaa ctattatgcg
    61141 cgccggaacc gcgacggtga aatatccctt cgcgccactg gaggtcagcc caggctttcg
    61201 cgggaaaccg gacctgatgc ccagccaatg tattgcctgc ggtgcctgcg cctgtgcttg
    61261 tccggcaaat gcgctgacta tccagaccga cgaccagcaa aattcgcgca cctggcagct
    61321 ctatctgggg cgttgtattt actgcggacg ttgtgaagaa gtgtgcccga ccagagccat
    61381 ccagcttacc aataactttg aactgaccgt cacccataaa gccgatctct atacccacgc
    61441 gacgttccat ctacaacgtt gcagccgttg cgaacgcccg tttgccccgc aaaaaaccgt
    61501 cgcactggct gctgaattgt tggcacagca acaaaatgct ccacaaaacc gcgaaatgtt
    61561 atgggcgcaa gcgagcgtct gcccggagtg caaacaacgc gcaacgctga tcaacgacga
    61621 tacagatgta ccgctggtgg ctaaggagca gctatgagtc cagtgcttac gcaatatgtc
    61681 agccagccca tcacgctgga cgagcaaacg caaaagatga agcggcattt gctacaggat
    61741 atccgtcgct cggcttacgt ttatcgcatc gattgcggcg gctgcaacgc ctgtgaaatc
    61801 gaaatttttg ctgccatcac ccccgtattc gacgcagaac gttttggcat taaggttgtt
    61861 tcatcaccgc gtcacgccga tattttgtta tttactggcg cagtcacccg ggcgatgcgt
    61921 atgcctgcac ttcgggcgta tgagtctgcc cccgatcata aaatctgtgt ttcctacggc
    61981 gcgtgcggtg tcggcggcgg tattttccac gatctctaca gcgtctgggg cggcagcgac
    62041 accattgtcc ccattgatgt ttggatcccc ggctgcccgc caacaccggc cgccaccatt
    62101 cacggtttcg ccgtggcgct cggtttgctg caacagaaga ttcacgcggt ggattatcgc
    62161 gatcccaccg gggtgactat gcaaccgttg tggccgcaga tcccgccatc acagcgtatc
    62221 gccattgagc gagaagcgcg gcggctggcg ggctatcgtc aggggcgaga aatttgcgat
    62281 cggctcctgc gccatttaag cgacgatcct acaggaaatc gggttaacac ctggttgcgc
    62341 gatgccgacg atccacgtct caatagtatc gttcagcaac tctttcgcgt actccggggg
    62401 ttacatgact gaagagtgcg gggaaattgt tttctggacg ctgcgaaaaa agtttgtcgc
    62461 cagtagcgac gagatgccgg aacacagctc tcaggtaatg tattactcgc tggctatcgg
    62521 tcatcacgtt ggcgtgattg attgtctgaa tgtcgccttc cgctgcccac tgacggaata
    62581 cgaagattgg cttgcactgg tcgaagagga gcaagcccga cgtaagatgc tgggggtgat
    62641 gacttttggt gagattgtta ttgacgccag ccacaccgcc ctgttgaccc gggcattcgc
    62701 gccactggca gatgacgcga cgtctgtgtg gcaagcgcgt agcattcaat tcattcatct
    62761 gttggatgaa attgtgctgg aaccggctat ctatctgatg gccagaaaaa ttgcgtgaga
    62821 aggatttctc attaataagg actgttgatg gctatgtcag acgaagcgat gtttgccccg
    62881 ccgcaaggaa taacaattga agcggtaaac ggaatgctcg cggagcggtt agcgcagaaa
    62941 cacggcaagg cgtctttatt acgcgccttc atcccgctgc cgccgccgtt cagcccggta
    63001 caacttattg aactgcatgt tctcaaaagc aacttctatt accgctacca tgatgatggc
    63061 agcgatgtga cggcaacaac agagtatcag ggcgagatgg tcgattattc gcgttacgcc
    63121 gtccttctcg gcagtagtgg aatggcggag ctacgcttta ttcgtaccca cggcagccgt
    63181 tttactcccc aggattgcac actgtttaac tggctggcac gcattatcac cccggttctg
    63241 caatcatggc tcaatgatga agcacagcag gtggcgctgc gtttgctgga gaaagatcgc
    63301 gatcatcatc gggtactggt ggatatcact aatgcagtgc tgtcacatct tgatctcgac
    63361 gatctgatcg ctgacgtcgc tcgtgagatc catcattttt tcggtctggc ttcagtcagt
    63421 atggtactgg gcgatcatcg aaagaacgag aagttcagcc tgtggtgcag cgatctttct
    63481 gcctcacatt gtgcgtgtct gccacacaat atgcctggcg acagtgtatt gctgacacaa
    63541 acgctacaaa cccgacaacc gaccttgacg caccgtgcag atgatctgtt tctctggcaa
    63601 cgcgacccgt tattactctt acttgcatct aacggctgcg aatctgcgct ccttataccg
    63661 cttacctttg gcaaccatac accgggtgca ttgttgctgg cgcatacctc ttccactctc
    63721 tttagtgagg aaaactgcca gctactacaa cacatagccg atcgcatcgc tattgccgtt
    63781 ggcaatgccg atgcctgccg tagaatgacc gatttgcagg aaagtttgca gcaagaaaac
    63841 caccagctta gcgagcagct cctttcgaat ctgggcgtcg gtgacattat ctatcaaagc
    63901 caggcaatgg aagacctgct ccagcaggta gatattgtgg cgaagagcga cagtacggtg
    63961 ttgatttgcg gtgaaaccgg aaccggcaaa gaggtgatcg ccagagcgat ccatcaactt
    64021 agcccgcgac acgacaagcc gctggtcaaa atcaactgcg ctgccatccc cgccagtctt
    64081 ctggaaagtg agttattcgg tcatgacaaa ggggcgttta ctggtgcgat taatacccat
    64141 cgtggtcgtt ttgaaattgc cgatggcggc acgttgtttc tcgatgaaat tggcgatctg
    64201 ccgttagaac ttcagcctaa actgctgcgc gtattgcagg aacgggagat tgagcgtctc
    64261 ggcgggagta gaacgatccc ggtaaatgtc agagtcattg ccgccaccaa ccgtgatttg
    64321 tggcaaatgg ttgaagatcg ccagtttcgc agcgatctct tttatcgcct gaatgtcttc
    64381 ccactggaat tgcctccgct gcgcgaccgt ccggaagata tccctctttt agcaaagcat
    64441 ttcacgcaaa aaatggcgcg ccatatgaat cgttcaattg acgccatccc gaccgaggcg
    64501 ctacgccagt tgatgtcgtg ggactggccg ggcaacgtgc gcgagctgga aaacgtgatt
    64561 gagcgagcgg tactgctgac tcgcggtaac agtctgaatt tacatcttaa tgtccgacaa
    64621 agccgtttac tgccgacgct aaatgaagat tcagcgcttc gcagttcaat ggcgtagttg
    64681 ctgcacccga cgacgccaga gaatgacgaa gaagaacgtc agcgcattgt tcaggtattg
    64741 cgagaaacca atggcattgt tgccgggccc cgtggcgcgg cgacacgatt agggatgaag
    64801 cgcaccacgc tgctgtcacg aatgcagcgt ctgggaatct cggttcgcga ggtgttgtaa
    64861 tctgcttttg caggagtatg catgagaaac aaactctctt tcgacttgca gttgagcgcc
    64921 agaaaagcgg caatcgctga acggattgcc gcccataaaa ttgcccgcag taaagtgtcg
    64981 gtctttttaa tggcgatgtc cgctggcgtg tttatggcga tgtccgctgg cgtgtttatg
    65041 gcgatcggat ttacttttta cctttccgtt atcgccgatg ccccgtcttc ataggcatta
    65101 acccatctgg tgggcggcct ttgctttaca cttggcttta ttttgctggc ggtttgcggc
    65161 accagcctgt tcacctcgtc ggtaatgacg gtaatggcaa aaagtcgggg cgttattagt
    65221 tggcgaactt ggctgattaa cgcacttctg gtggcctgcg gtaatctggc aggtattgcc
    65281 tgtttcagtt tgttaatctg gttttccggg ctggtgatga gtgaaaacgc gatgtgggga
    65341 gtcgcgattt tacactgcgc cgagggcaaa atgcatcata catttactga atctgtcagc
    65401 ctcggcatta tgtgcaatct gatggtttgc ctggcgctgt ggatgagtta ttgcggacgt
    65461 tcgttatgcg acaaaatcgt cgccatgatt ttgtccatca ccctgtttgt cgccagtggc
    65521 tttgagcact gtatcgccaa tttgtttgtg attccgttcg ccattgccat tcgccatttc
    65581 gcccctacct ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
    65641 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
    65701 gcagtgctgg tgagtatttg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
    65761 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
    65821 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
    65881 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
    65941 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
    66001 acaccgcgaa gcagctccag aattcagtac ctgcgccaaa ctggaatagc gccacgccaa
    66061 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
    66121 gcactgccag cagcagcgaa tagttcagcc cgaagagcaa gaaccccagc caggtggcga
    66181 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
    66241 tctccttcca cacctgtcct gccagcccac ggttacgcgg cagcacccgg cgaacggcgt
    66301 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
    66361 agacggctat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
    66421 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
    66481 ttaacaccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
    66541 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
    66601 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
    66661 agcagccaat agattgcagg cgcacggttg gccattccag caaataagcc agcacaatag
    66721 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
    66781 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
    66841 acatttcgag cataaagacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
    66901 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca
    66961 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gaggtgggaa cgatatttca
    67021 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
    67081 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
    67141 tttgccgaca gcgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
    67201 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
    67261 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
    67321 catgccaatt cggttaagac accgtttcat ttttttctga tcaacaacga cgaaattaac
    67381 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
    67441 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacgtcac ccaacgtcac
    67501 ctggcgcgag cgatggaaga tcaacaacgc agcgcgccgc tgacctgggt cggcgcgtta
    67561 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
    67621 ctggcgggaa cgcgccaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
    67681 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cacaggcgat gccgaccttc
    67741 ctggaaaaat tgctcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact
    67801 cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgcccg
    67861 atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat
    67921 aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt
    67981 cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac
    68041 gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat
    68101 ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc
    68161 ctgaaaaatg cccgcgattt acgcaccaat ccggtgttgc agctcaacct ggcgaacgct
    68221 tatctgcaag gcggtcaacc acaagaagcg gccaatattc tgaatcgcta cacctttaat
    68281 aataaagatg acagcaacgg ctgggatttg ctggcacagg cggaagccgc gctaaataac
    68341 cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag
    68401 gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg
    68461 cgttacgatg cgcgcatcga ccagttgcgc cagctgcaga aacgctttaa gccttatacc
    68521 aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc
    68581 cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag
    68641 aagtggtgct ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa
    68701 tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac
    68761 taaacctggc tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc
    68821 cgaagctgat ggagcgcccg attatggtcg cgaatggcaa agcgcggatt ggtcgtccac
    68881 cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg
    68941 ccggatgcga cgcttgccac gtcttatccg gcctacgaat actatggatt aacttcgcgt
    69001 aggtcaggcg ttcgcgccgc atccgacaat aaacacctta tctacaactt cagaatttct
    69061 ttcacaaacg gaatggtcag cttacgttgc gcggtaatcg acgcacgatc caactgatcc
    69121 aacgtcataa atagcgtgcg catttctctg tcgagccgct tcagcaagaa acgccccaca
    69181 tcttccggca gttcaaaacc acgcaaacgc gcgcgtaact gtagcgcctg caacttatct
    69241 tcatcagaaa gtggctgcaa tttgtagatc tgcccccagt cgagacgcga cgcgagatcc
    69301 ggtaatccca gattcaactg ccgcggtgga cgatcgccgg tgatcaacaa ccgtgttttg
    69361 cccgattcca gaattcgatt gtagagatcg aaaatcgcca tctcccacaa ctcatcgcct
    69421 gcaatacact caatgttgtc gatacagacc agcgacaaat gctccatacc gtcgagcact
    69481 tccggaacaa accaggtgcg tttatccagc gggacatagc ccaccgcatc gccacgctgc
    69541 gacaattccg cgcaagccgc gtgcagcaga tggctgcgcc ccgcgccttc gcgtgcccag
    69601 agatagatgt aaccgctatg ttcctgacgc agcacgtttt gcagcgcggc cagtaaagag
    69661 gagttatccc ccggccagaa acttgcaaag gtttcgtcgt caggaagata aagtggcaaa
    69721 gagagctgtg ccggtgtgtt cagagatacc tcaaccagga tttcacaaaa tcgcgagaag
    69781 tttaccacag aatcccatga tgtttgaacc gggcagcaac actgcccggt cggtacatta
    69841 tttgtctgtt atatccgcgt cttctgcgtc cagcaccact tcttcagggc gcagcacact
    69901 gatcagtttg aaaatcaaac ttaagccgat accgacgatg gtcgccagcg ccataccttt
    69961 taactccgcc gcgccgatgt ttaccttcgc gccactgacg ccgatgatca aaatcacgga
    70021 agtcaggatc aggttctgtg ctttgttgta atccactttc gattcgatca aaacacgaat
    70081 accggaagca ccaatgacac cataaagcag cagcgaaacg ccgcccatca ccggcaatgg
    70141 gatcatctgg atagcggcag ccagtttacc gacgcaggaa agcaggatag cgaaaatcgc
    70201 cgccccgccg ataacccagg tactgtaaac acgggtgatc gccatcacgc caatgttttc
    70261 tccgtaagtg gtatttggcg tagagccaaa gaagccggag atcacggtcg acaggccgtt
    70321 agcaaacatc gaacggtgca gacctggatc gcgcagcaga tcttttttga cgatattagc
    70381 cgttactacc aggtgcccta cgtgttcggc aataaccact aacgccgctg gcagaatagt
    70441 cagaatggca aaccactcga agcgcggcgt atagagggtt ggcagtgcaa accagtgagc
    70501 attaataatc ggcgtggtat cgacaattcc cattgcgaaa gagagcgcgt accccaccag
    70561 cacgccaatt aaaatcggga taattgccag gaaaccacga aacagcacgg aacctaaaac
    70621 cgtgaccgcc agggtggtaa tagagatgat gatggttttg gagtttggcg tttgcccttc
    70681 agccgggagt aaacccgcca taccggcagc tacgcccgcc agctccagac cgatgacggc
    70741 aacgattgcg cccattgccg caggtggaaa cagcacgtcc aaccagccgg tccccgcttt
    70801 cttcacgata aaagaaacca ggcagaacag cacgccgcac ataataaagc cgcccagcgc
    70861 gacttcatac cctaacggca acagtaacaa taccggtgaa ataaaggcaa agctggaacc
    70921 aagataagcc ggaattttcc ctttacagat gaagagatac agcagcgttc caataccgtt
    70981 aaataacagt acagtcgccg ggttaatatg aaataagacg ggcaccagga cggttgcacc
    71041 aaacatggcg aacaaatgtt gcaaactaag cgggattgtc tgtaaaagtg gcggtctttc
    71101 actcaccccg atagcacggc gcgtcatagt attatcctct gtattatgtg ttataggcgc
    71161 tttactcaaa aaaaagccga ctcttaaagt cggctttaat tatttttatt ctttatttcg
    71221 taccaaagat tttgtcaccg gcatcgccga ggcccggaat aatgtatccg tgctcgttca
    71281 gtccctggtc aatcgatgca gtatacagtt cgacgtccgg gtgcgctttt tccagcgcag
    71341 cgataccttc tggcgcagcg accagcacca gaactttgat gctgctgcag cccgcttttt
    71401 tcagcaggtc gatggtcgcg ataacggaac caccggttgc cagcattggg tcaacgatca
    71461 gcgccatacg ctcatcgatg ttagaaacca gtttctggaa gtacggtacc ggctccagcg
    71521 tttcttcatt acggtacata ccgacaacgc tgatgcgcgc gctcggaacg ttttccagca
    71581 caccgtccat cataccaaga cccgcacgca gaattggcac aacggtaatt ttcttacctt
    71641 tgatctggtc gatttctacc gggccgttcc agccttcgat agttactttt tccgtttcga
    71701 ggtcggcggt cgcttcgtaa gtcagcaggc tacccacttc ggaagcgagt tcgcgaaagc
    71761 gcttggtgct gatatcttgc tcacgcatca gtcccagctt gtgtttgacg agtgggtgtt
    71821 tgacttccac gatcttcata ctctttctcc tttgaggggc agccacaaaa aaatcgacgg
    71881 attatacctc ctttcttcag ggcggcaata ttattttcgt tgactttagt caaaatgata
    71941 acagtttgag ataaagttat tttatattca aatggttatg aaagaagatt attccatccg
    72001 aaaactaacc tttgccctgg cacaagtctt ctttcgccgc gcgcccgggg aaaagacgtg
    72061 caaaaaggtt gtgtaaaaca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga
    72121 attttatttt tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct
    72181 cttagctaca aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc
    72241 aaaggcgtag tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt
    72301 gcgctgtgtg cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc
    72361 gtaggtacca agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat
    72421 ctggtcgcca tgtgcgttaa tgacctggtg gtgcaaggtg cggaaccgct gtttttcctc
    72481 gactattacg caaccggaaa actggatgtt gataccgctt cagcggtgat cagcggcatc
    72541 gcggaaggtt gtctgcaatc gggctgttct ctggtgggtg gcgaaacggc agaaatgccg
    72601 gggatgtatc acggtgaaga ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa
    72661 tcagaaatca tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc
    72721 agcggtccgc actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt
    72781 gatccgcaaa ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc
    72841 cgcatttacg tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg
    72901 cacctgaccg gcggcggctt ctgggaaaac attccgcgcg tattgccaga taatacccag
    72961 gcagtgattg atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca
    73021 ggtaacgttg agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc
    73081 gccctgcctg ctccggaagt ggacaaagcc ctcgccctgc taaatgccaa cggtgaaaac
    73141 gcgtggaaaa tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa
    73201 tgaatattgt ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct
    73261 gtaaaaccaa caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt
    73321 tcggccttga acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt
    73381 ttgacagtcg tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg
    73441 atgtggtcgt gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg
    73501 ccgggcgttt gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc
    73561 atcgtcaggc gctggaaaat ggcgatgaag agcacggtac atcggtgcat ttcgtcaccg
    73621 atgaactgga cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata
    73681 cggaagatga catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga
    73741 ttagctggtt tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc
    73801 aacgtctgcc gccgcagggc tacgctgccg acgagtaatg cccccgtagt taaagctcca
    73861 gctctgccgc tggcgttttt caattcacct gtaaatcgca agctccagca gtttttttcc
    73921 cccttttctg gcatagttgg acatctgcca atattgctcg ccataatatc caggcagtgt
    73981 cccgtgaata aaacggagta aaagtggtaa tgggtcagga aaagctatac atcgaaaaag
    74041 agctcagttg gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc
    74101 cgctgattga aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata
    74161 aagtccgctt cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaacg
    74221 ctcattcccg ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggaat
    74281 tcgacggcct ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta
    74341 atgaacgcca gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc
    74401 tgcgtcagca cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga
    74461 aagatgatta cacctatctg gcggtggaaa ttatccgtag cgatagcatc cgttacgcgc
    74521 tgctggagat cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc
    74581 gtcgacgcaa gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt
    74641 tcaaaggctt ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg
    74701 ccgaatacga tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc
    74761 tcaagcagcg tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg
    74821 cgctggttga agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg
    74881 gcggtcgtta tcataatttt aaagacttta ttaatttccc caatgtcggc aaagccaatc
    74941 tggtgaacaa accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca
    75001 atggttttga tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg
    75061 agcatgtgct ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa
    75121 ttaacattta ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac
    75181 ataacggtaa gaaagtcact gtggtggttg agttacaggc gcgtttcgac gaagaagcca
    75241 acattcactg ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg
    75301 ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtcc
    75361 gttacgcaca catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact
    75421 attcgttgct gaccgcagat gcgcgtatca ccaacgaagt acggcgggta tttaacttta
    75481 ttgaaaaccc ataccgcccg gtgacatttg attatttaat ggtgtcaccg caaaactctc
    75541 gccgtctgtt atatgaaatg gtagaccgcg aaatcgccaa cgcgcagcaa gggctgccca
    75601 gtggtatcac cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg
    75661 cggcctccag ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc
    75721 ccaatctgga aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg
    75781 aacatgaccg ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg
    75841 ccgactggat gacgcgcaat attgattatc gtattgaagt ggcgacaccg ctgctcgatc
    75901 cgcgcctgaa gcagcgggtg ctggacatca tcgacatatt gttcagcgat acggtcaaag
    75961 cacgttatat cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag
    76021 tacgggcgca gttggcgatt tacgactaca tcaaatcact cgaacaacct gaataaccct
    76081 atgccaatac acgataaatc ccctcgtccg caggagtttg ctgcggtcga tcttggttca
    76141 aacagttttc acatggtcat agcccgtgtg gtagatggtg ccatgcagat tattggccgc
    76201 ctgaaacagc gggtgcatct ggcggacggc ctggggccag ataatatgtt gagtgaagag
    76261 gcaatgacgc gcggtttgaa ctgtctgtcg ctgtttgccg aacggctaca agggttttct
    76321 cctgccagcg tctgtatagt cggcacccat acgctgcgtc aggcgctgaa cgccactgac
    76381 tttctgaaac gcgcggaaaa ggtcattccc tacccgattg aaattatttc cggtaatgaa
    76441 gaagcccgtc tgatttttat gggcgtggaa catacccaac cggaaaaagg tcgcaaactg
    76501 gttattgata ttggcggcgg atctacggaa ctggtgattg gtgaaaattt cgaacctatt
    76561 ctcgttgaaa gccgccggat gggttgtgtc agctttgccc agctttattt tcctggcggg
    76621 gtcatcaata aagagaattt tcagcgcgct cgcatggcgg cagcacaaaa actggaaact
    76681 ttaacctggc aattccgtat tcagggctgg aacgttgcaa tgggcgcttc cggtaccata
    76741 aaagccgccc atgaagtgtt aatggaaatg ggcgagaaag acgggataat taccccggaa
    76801 cgtctggaaa aactggtaaa agaaatttta cgtcaccgta atttcgcatc gctgagttta
    76861 ccgggtcttt ccgaagagcg gaaaacagtc ttcgttcctg gactggcgat tttatgtggt
    76921 gtgtttgatg ctttagccat ccgtgaactg cgcctttctg acggggcgtt acgcgaaggc
    76981 gtactgtatg aaatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc
    77041 agcctcgcca accagtatca catagacagc gaacaggccc gacgagtgct ggataccact
    77101 atgcaaatgt acgaacagtg gcgggaacag caaccgaagc tggcgcatcc gcaactggag
    77161 gcgctactgc gatgggccgc catgctgcat gaggtcgggt tgaatatcaa ccacagcggt
    77221 ttgcatcgcc actccgctta tattctgcaa aacagtgact tgccgggttt taatcaggaa
    77281 cagcagctga tgatggcgac actggtgcgc tatcaccgta aagcgattaa gctcgacgat
    77341 ctgccgcgct ttaccttgtt taagaagaaa cagttcctgc cactgataca gctattgcgc
    77401 cttggcgtat tactaaacaa tcaacgtcag gcaaccacca caccgccaac attgacactg
    77461 attactgatg acagtcactg gacactgcgt ttcccgcatg actggtttag tcagaatgcg
    77521 ctggtactgc ttgatctgga aaaggagcaa gaatactggg aaggcgtggc tggctggcgg
    77581 ttgaaaattg aagaagaaag taccccagaa atcgccgctt aatgctttgc gggccgacga
    77641 gaaatgtcgg cccgcattat tcaggcactt tcgcgaatgg gttcgatttc attcagcgta
    77701 tcaattaacg gttgcggctt accaataaga taaccctgca tataatcgat ccccaaagag
    77761 agcaccgcct cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc
    77821 attcgcgcca gatggcaaat cgacgccaca atctgataat ccagactatt tgacacaata
    77881 ttgc
//
LOCUS       DAJVPT010000003        70919 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVPT010000003 DAJVPT010000000
VERSION     DAJVPT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(740..2326)
                     /locus_tag="OQM47_000183"
     CDS             complement(740..2326)
                     /locus_tag="OQM47_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCS1478478.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2681..4852)
                     /gene="glcB"
                     /locus_tag="OQM47_000184"
     CDS             complement(2681..4852)
                     /gene="glcB"
                     /locus_tag="OQM47_000184"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1478479.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4874..5278)
                     /locus_tag="OQM47_000185"
     CDS             complement(4874..5278)
                     /locus_tag="OQM47_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCS1478480.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5283..6502)
                     /gene="glcF"
                     /locus_tag="OQM47_000186"
                     /pseudo
     CDS             complement(5283..6502)
                     /gene="glcF"
                     /locus_tag="OQM47_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6513..7565)
                     /gene="glcE"
                     /locus_tag="OQM47_000187"
     CDS             complement(6513..7565)
                     /gene="glcE"
                     /locus_tag="OQM47_000187"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1478481.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7565..9064)
                     /gene="glcD"
                     /locus_tag="OQM47_000188"
                     /pseudo
     CDS             complement(7565..9064)
                     /gene="glcD"
                     /locus_tag="OQM47_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9315..10085
                     /gene="glcC"
                     /locus_tag="OQM47_000189"
     CDS             9315..10085
                     /gene="glcC"
                     /locus_tag="OQM47_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCS1478482.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10209..11350)
                     /locus_tag="OQM47_000190"
                     /pseudo
     CDS             complement(10209..11350)
                     /locus_tag="OQM47_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11712..13442
                     /locus_tag="OQM47_000191"
     CDS             11712..13442
                     /locus_tag="OQM47_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCS1478483.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13439..14353
                     /locus_tag="OQM47_000192"
     CDS             13439..14353
                     /locus_tag="OQM47_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1478484.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14386..14634
                     /locus_tag="OQM47_000193"
     CDS             14386..14634
                     /locus_tag="OQM47_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1478485.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14634..15805
                     /locus_tag="OQM47_000194"
                     /pseudo
     CDS             14634..15805
                     /locus_tag="OQM47_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15841..16920)
                     /gene="lptG"
                     /locus_tag="OQM47_000195"
     CDS             complement(15841..16920)
                     /gene="lptG"
                     /locus_tag="OQM47_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1478486.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16917..17987)
                     /gene="lptF"
                     /locus_tag="OQM47_000196"
     CDS             complement(16917..17987)
                     /gene="lptF"
                     /locus_tag="OQM47_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1478487.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18018..18578)
                     /locus_tag="OQM47_000197"
     CDS             complement(18018..18578)
                     /locus_tag="OQM47_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1478488.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18590..19414)
                     /locus_tag="OQM47_000198"
     CDS             complement(18590..19414)
                     /locus_tag="OQM47_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478489.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19414..20763)
                     /locus_tag="OQM47_000199"
     CDS             complement(19414..20763)
                     /locus_tag="OQM47_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1478490.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20809..21567)
                     /locus_tag="OQM47_000200"
     CDS             complement(20809..21567)
                     /locus_tag="OQM47_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478491.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21599..22312)
                     /locus_tag="OQM47_000201"
     CDS             complement(21599..22312)
                     /locus_tag="OQM47_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1478492.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22486..23178
                     /locus_tag="OQM47_000202"
     CDS             22486..23178
                     /locus_tag="OQM47_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1478493.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23227..24726)
                     /gene="pitB"
                     /locus_tag="OQM47_000203"
     CDS             complement(23227..24726)
                     /gene="pitB"
                     /locus_tag="OQM47_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1478494.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25019..26878)
                     /gene="gss"
                     /locus_tag="OQM47_000204"
     CDS             complement(25019..26878)
                     /gene="gss"
                     /locus_tag="OQM47_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1478495.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27083..27949
                     /gene="yghU"
                     /locus_tag="OQM47_000205"
     CDS             27083..27949
                     /gene="yghU"
                     /locus_tag="OQM47_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1478496.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28072..28320)
                     /gene="hybG"
                     /locus_tag="OQM47_000206"
     CDS             complement(28072..28320)
                     /gene="hybG"
                     /locus_tag="OQM47_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1478497.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28454..28795)
                     /gene="hypA"
                     /locus_tag="OQM47_000207"
     CDS             complement(28454..28795)
                     /gene="hypA"
                     /locus_tag="OQM47_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1478498.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28788..29276)
                     /gene="hybE"
                     /locus_tag="OQM47_000208"
     CDS             complement(28788..29276)
                     /gene="hybE"
                     /locus_tag="OQM47_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1478499.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29269..29763)
                     /locus_tag="OQM47_000209"
     CDS             complement(29269..29763)
                     /locus_tag="OQM47_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1478500.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29763..31466)
                     /gene="hybC"
                     /locus_tag="OQM47_000210"
     CDS             complement(29763..31466)
                     /gene="hybC"
                     /locus_tag="OQM47_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1478501.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31463..32641)
                     /gene="hybB"
                     /locus_tag="OQM47_000211"
     CDS             complement(31463..32641)
                     /gene="hybB"
                     /locus_tag="OQM47_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1478502.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32631..33617)
                     /gene="hybA"
                     /locus_tag="OQM47_000212"
     CDS             complement(32631..33617)
                     /gene="hybA"
                     /locus_tag="OQM47_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1478503.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33620..34738)
                     /gene="hybO"
                     /locus_tag="OQM47_000213"
     CDS             complement(33620..34738)
                     /gene="hybO"
                     /locus_tag="OQM47_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1478504.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34927..35214)
                     /locus_tag="OQM47_000214"
     CDS             complement(34927..35214)
                     /locus_tag="OQM47_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1478505.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35333..36220)
                     /locus_tag="OQM47_000215"
     CDS             complement(35333..36220)
                     /locus_tag="OQM47_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1478506.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36377..37417
                     /gene="gpr"
                     /locus_tag="OQM47_000216"
     CDS             36377..37417
                     /gene="gpr"
                     /locus_tag="OQM47_000216"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1478507.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37457..37951)
                     /locus_tag="OQM47_000217"
     CDS             complement(37457..37951)
                     /locus_tag="OQM47_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1478508.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38142..39026
                     /gene="yghA"
                     /locus_tag="OQM47_000218"
     CDS             38142..39026
                     /gene="yghA"
                     /locus_tag="OQM47_000218"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1478509.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39298..39723)
                     /gene="exbD"
                     /locus_tag="OQM47_000219"
     CDS             complement(39298..39723)
                     /gene="exbD"
                     /locus_tag="OQM47_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1478510.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39730..40464)
                     /gene="exbB"
                     /locus_tag="OQM47_000220"
     CDS             complement(39730..40464)
                     /gene="exbB"
                     /locus_tag="OQM47_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1478511.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40716..41903
                     /gene="metC"
                     /locus_tag="OQM47_000221"
     CDS             40716..41903
                     /gene="metC"
                     /locus_tag="OQM47_000221"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1478512.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42043..42702
                     /locus_tag="OQM47_000222"
     CDS             42043..42702
                     /locus_tag="OQM47_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1478513.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42742..43641)
                     /gene="yqhC"
                     /locus_tag="OQM47_000223"
     CDS             complement(42742..43641)
                     /gene="yqhC"
                     /locus_tag="OQM47_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1478514.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43835..44998
                     /gene="yqhD"
                     /locus_tag="OQM47_000224"
     CDS             43835..44998
                     /gene="yqhD"
                     /locus_tag="OQM47_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1478515.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45103..45930
                     /gene="dkgA"
                     /locus_tag="OQM47_000225"
     CDS             45103..45930
                     /gene="dkgA"
                     /locus_tag="OQM47_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1478516.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46130..47056
                     /locus_tag="OQM47_000226"
     CDS             46130..47056
                     /locus_tag="OQM47_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1478517.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47107..47364
                     /gene="yqhH"
                     /locus_tag="OQM47_000227"
     CDS             47107..47364
                     /gene="yqhH"
                     /locus_tag="OQM47_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1478518.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47407..49626)
                     /locus_tag="OQM47_000228"
     CDS             complement(47407..49626)
                     /locus_tag="OQM47_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1478519.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49737..51149)
                     /gene="ftsP"
                     /locus_tag="OQM47_000229"
     CDS             complement(49737..51149)
                     /gene="ftsP"
                     /locus_tag="OQM47_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1478520.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51224..51961)
                     /gene="plsC"
                     /locus_tag="OQM47_000230"
                     /gene_synonym="parF"
     CDS             complement(51224..51961)
                     /gene="plsC"
                     /locus_tag="OQM47_000230"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1478521.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52195..54453)
                     /gene="parC"
                     /locus_tag="OQM47_000231"
     CDS             complement(52195..54453)
                     /gene="parC"
                     /locus_tag="OQM47_000231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1478522.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(54999..55481)
                     /locus_tag="OQM47_000232"
     CDS             complement(54999..55481)
                     /locus_tag="OQM47_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1478523.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55534..55926)
                     /gene="ygiW"
                     /locus_tag="OQM47_000233"
     CDS             complement(55534..55926)
                     /gene="ygiW"
                     /locus_tag="OQM47_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1478524.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56078..56737
                     /gene="qseB"
                     /locus_tag="OQM47_000234"
     CDS             56078..56737
                     /gene="qseB"
                     /locus_tag="OQM47_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1478525.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56734..58008
                     /gene="qseC"
                     /locus_tag="OQM47_000235"
     CDS             56734..58008
                     /gene="qseC"
                     /locus_tag="OQM47_000235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1478526.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58054..58386)
                     /locus_tag="OQM47_000236"
     CDS             complement(58054..58386)
                     /locus_tag="OQM47_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1478527.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58705..59286
                     /gene="mdaB"
                     /locus_tag="OQM47_000237"
     CDS             58705..59286
                     /gene="mdaB"
                     /locus_tag="OQM47_000237"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1478528.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59317..59631
                     /locus_tag="OQM47_000238"
     CDS             59317..59631
                     /locus_tag="OQM47_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1478529.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59679..61571)
                     /gene="parE"
                     /locus_tag="OQM47_000239"
     CDS             complement(59679..61571)
                     /gene="parE"
                     /locus_tag="OQM47_000239"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1478530.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61600..62181)
                     /gene="yqiA"
                     /locus_tag="OQM47_000240"
     CDS             complement(61600..62181)
                     /gene="yqiA"
                     /locus_tag="OQM47_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1478531.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62181..63008)
                     /gene="cpdA"
                     /locus_tag="OQM47_000241"
     CDS             complement(62181..63008)
                     /gene="cpdA"
                     /locus_tag="OQM47_000241"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1478532.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63033..63455)
                     /locus_tag="OQM47_000242"
     CDS             complement(63033..63455)
                     /locus_tag="OQM47_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1478533.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63456..64085)
                     /gene="nudF"
                     /locus_tag="OQM47_000243"
     CDS             complement(63456..64085)
                     /gene="nudF"
                     /locus_tag="OQM47_000243"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1478534.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64290..65771
                     /gene="tolC"
                     /locus_tag="OQM47_000244"
     CDS             64290..65771
                     /gene="tolC"
                     /locus_tag="OQM47_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1478535.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65919..66590
                     /locus_tag="OQM47_000245"
     CDS             65919..66590
                     /locus_tag="OQM47_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1478536.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66596..67756
                     /locus_tag="OQM47_000246"
     CDS             66596..67756
                     /locus_tag="OQM47_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1478537.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67794..68582)
                     /gene="ygiD"
                     /locus_tag="OQM47_000247"
     CDS             complement(67794..68582)
                     /gene="ygiD"
                     /locus_tag="OQM47_000247"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1478538.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68725..69498
                     /gene="zupT"
                     /locus_tag="OQM47_000248"
     CDS             68725..69498
                     /gene="zupT"
                     /locus_tag="OQM47_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1478539.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69556..69726)
                     /locus_tag="OQM47_000249"
     CDS             complement(69556..69726)
                     /locus_tag="OQM47_000249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478540.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69987..70640)
                     /gene="ribB"
                     /locus_tag="OQM47_000250"
     CDS             complement(69987..70640)
                     /gene="ribB"
                     /locus_tag="OQM47_000250"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1478541.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70744..70892)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT        17652 a        18931 c        17548 g        16788 t
ORIGIN      
        1 acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca caaacaggca
       61 ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca taatcaccaa
      121 attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg ttggagttaa
      181 aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg gatgatttcg
      241 aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta acgaacactg
      301 ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt ggagataaat
      361 tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa aatcagcttt
      421 gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt gttctagtga
      481 aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga agcaatgtaa
      541 taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca gaagccaagt
      601 tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag aagccaatcg
      661 taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt gatatttacc
      721 ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac gcctgcagca
      781 gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac agttcagatt
      841 ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg gagatcatct
      901 tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag acgttgattt
      961 gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg tccgacccgg
     1021 taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc acgcctgtac
     1081 ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg aaggcgaatg
     1141 ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt tcggcaaaga
     1201 cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc gccgcaataa
     1261 aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc attggcgttg
     1321 gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg atctggaaat
     1381 taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac ggcttcatgg
     1441 tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga atgatttgcc
     1501 ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc accatcgcac
     1561 ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag actttaagga
     1621 ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt tccggaccaa
     1681 tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc agcgccgctg
     1741 gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag aacggcacaa
     1801 gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac ggatcgattc
     1861 ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc accgtcgcac
     1921 caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt aacacctgca
     1981 aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac tgcccgctgg
     2041 caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt ggccataatc
     2101 cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt ttaaaggcga
     2161 atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt cctttcagac
     2221 gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc agagcggata
     2281 gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg ttcagtctct
     2341 tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg actgaactgt
     2401 aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg ctacctctgg
     2461 cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag agtatcgtta
     2521 agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt taatattgtt
     2581 aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag ttactatcat
     2641 agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt tctttttcgc
     2701 gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc acgccgagga
     2761 agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc gccatcggac
     2821 gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc tccagcgacg
     2881 cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata tgctggctgg
     2941 agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc ggcacttttg
     3001 aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc tgcacgttgt
     3061 tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc accggaatag
     3121 tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg gcaatgttgg
     3181 cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta gcagcggttg
     3241 gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc tggctgtaca
     3301 tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct ttaccgcgca
     3361 gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc cacggcgtcg
     3421 atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc atttcatcgc
     3481 cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc tgagcgatac
     3541 agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt ttcagggtat
     3601 tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg aacgccactt
     3661 cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc gagtttttct
     3721 gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga ataccttccg
     3781 ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga cccacgttgc
     3841 ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg gcggtgtact
     3901 gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt ttctcttgca
     3961 gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct tccgcatcaa
     4021 ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg acgataacat
     4081 cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt tgcagttcaa
     4141 tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg ccacggtaac
     4201 cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac tggatgcgta
     4261 attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt tccagcggta
     4321 gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc tgcggatcgt
     4381 agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac gcatcgtaca
     4441 gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt gccggaacca
     4501 ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt tccaccgtca
     4561 cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta taggccgctt
     4621 tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc tgaatgcgat
     4681 cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc tcatcaacat
     4741 tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca tccacaaaac
     4801 gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc attgtttctc
     4861 tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc tgcgcatcct
     4921 gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca acaacaaccg
     4981 gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta cgtccgttgt
     5041 tcaccatctc ttcatagccc ttagtttcac gacgccccag cgcggcggta cgcgctttct
     5101 cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac gccagcagat
     5161 gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc gcctcttcct
     5221 gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta gttttcattt
     5281 ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac agaggtacga
     5341 ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat ttccggtttg
     5401 ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc gggatgcgtt
     5461 aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc cgttaaggta
     5521 aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc atgttgtagg
     5581 gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt ttccagcggt
     5641 tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg cgctttatcg
     5701 gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc gccgcaaccg
     5761 ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca ggcatcaata
     5821 ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc caccgcgcca
     5881 caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag cactcgcgca
     5941 gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag cattaaaacc
     6001 cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc aggcagtttt
     6061 gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt cagcgcacgg
     6121 aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg ctccggcagt
     6181 gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt gtgatagcgc
     6241 acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc gagatgctcc
     6301 tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata gatgcgcccg
     6361 cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc ggtacaaaat
     6421 ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc tgccgcatct
     6481 cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat gcgaccgggg
     6541 ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg gaataaagga
     6601 gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg accgccagcg
     6661 ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca gcgtaacgcc
     6721 ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc gctgggtaat
     6781 gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc acgcaattgc
     6841 tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc ttttaccgat
     6901 ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa gccactaatg
     6961 ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag gctgatttcc
     7021 cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat ttcagtgagc
     7081 acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc ggcaacgttt
     7141 ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat gcgcgtgccg
     7201 aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag cccgcaggcg
     7261 accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg aagcatttgc
     7321 ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg agcggttatc
     7381 accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc cagcgtttgc
     7441 ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac cagcggcgtt
     7501 ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc acactcgcgt
     7561 agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca tgcatggcac
     7621 caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc aaaccatcgg
     7681 ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg aactgggcgc
     7741 acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg ccgccaactt
     7801 caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat tcaccaggtt
     7861 cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga aagacgttgg
     7921 caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg ccaggcaggg
     7981 cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt cctaccgccg
     8041 ggaacgcatt tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc tgtgccaaac
     8101 ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag tcttcctgta
     8161 cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg acgggataac
     8221 cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc tccagcccgc
     8281 cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt tctaccgagt
     8341 caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt accgtcactt
     8401 cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg tcaaaaccag
     8461 gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt tgcacttcaa
     8521 ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca ccggcatttt
     8581 ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc gcgtaataga
     8641 gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc tgcacgcgcg
     8701 cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc accaacaaca
     8761 cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca cgggtcacca
     8821 ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc atttgcttag
     8881 gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac tcgtaaggaa
     8941 tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc agtgccatca
     9001 gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg tacaagatgc
     9061 tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc gctgcttctg
     9121 tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc ggtcagacca
     9181 ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg atgtaacata
     9241 atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt cctacctgtg
     9301 cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt gcagagagta
     9361 tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc tcggaacgtc
     9421 gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg accgtgctgc
     9481 gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca cggcttaatc
     9541 gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga acgctgtacg
     9601 atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg gcaacgctgg
     9661 gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc gccgccagtg
     9721 agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct ttccatctcg
     9781 ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca ttgaccgatc
     9841 tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca cagcaaaaaa
     9901 agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg ctgccgcacg
     9961 tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg
    10021 agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac aaggtgggaa
    10081 tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac ttaacaggat
    10141 ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc gctaaacagg
    10201 atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc cgccccaatg
    10261 cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat ctccagcgca
    10321 tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc
    10381 gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt
    10441 cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg aaagagcgat
    10501 ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac gcgcagggtg
    10561 cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta agttggtcgc
    10621 ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc tgccagccag
    10681 agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga ttgatgcagc
    10741 gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga gagaaagtga
    10801 gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa cccagttgtt
    10861 caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt ggtgtgtcaa
    10921 aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc ccgctgatct
    10981 tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg gcaaaatcct
    11041 gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg taacgctcac
    11101 ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct ttttttgcca
    11161 cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa tgctcgttgc
    11221 gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat gacggaaaag
    11281 cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc atcagcagat
    11341 cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt tgcaacagca
    11401 acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat agtattaatt
    11461 aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt gtcaatttaa
    11521 ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt cataataaaa
    11581 acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat gaaaatacta
    11641 tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta tttttgatat
    11701 ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta cctatgcgct
    11761 atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt agagccggaa
    11821 tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag acattaaaaa
    11881 cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa ggcgaacgcg
    11941 tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc tgtcagtatg
    12001 ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg gattcctgga
    12061 gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc actggtgatg
    12121 agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat gttttaagcc
    12181 acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca gttccgaacg
    12241 atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc gtcattatca
    12301 cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt aaattacgcc
    12361 ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg gtcggctttc
    12421 tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag gattttgcca
    12481 tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt tccgttgcgc
    12541 cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc gctgaactgg
    12601 atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca gaacaactcc
    12661 atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc atgccgtgct
    12721 acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc ggggttgtgg
    12781 ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg ccgggtgcag
    12841 agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa catggtattg
    12901 aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat atttgcatct
    12961 ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac gagattgccg
    13021 cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat ctgtatgtca
    13081 ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg caggatattg
    13141 aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct tttgttaccg
    13201 cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa cgtcgcgggc
    13261 agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc gcggctatcg
    13321 atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc cgtgcggaag
    13381 cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa tccctggcat
    13441 aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata ttatcgataa
    13501 cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg ctcacgtcaa
    13561 tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta gcgagctggt
    13621 tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca aagaagagat
    13681 tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa aagagagcgg
    13741 cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg agctctcctg
    13801 gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg acgaaattac
    13861 gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt taaaaccgct
    13921 gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata cacaactctc
    13981 ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg ccgaaactgt
    14041 acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact ggcaacgcgt
    14101 acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca ttcctctgcc
    14161 ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag gtaaagaacc
    14221 tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg caagtaataa
    14281 ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg cattacgcaa
    14341 cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt aaatcgtgaa
    14401 atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg tgtggaaatt
    14461 aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt gatggatctc
    14521 ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct gctggatgtc
    14581 aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa ataatggggc
    14641 tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac aacgggttaa
    14701 caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg atcggcaata
    14761 aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat catgatgcca
    14821 tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt tctcgtatgg
    14881 caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag tttttcaatc
    14941 gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact
    15001 tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgt
    15061 ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga
    15121 acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat
    15181 ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct
    15241 cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg caacggggcg
    15301 cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag
    15361 taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg aagtgctgcg
    15421 ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt
    15481 gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa
    15541 cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag
    15601 cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct
    15661 tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac
    15721 gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca
    15781 ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt
    15841 ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg
    15901 taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat
    15961 ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat
    16021 cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt
    16081 caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc tgggttgccc
    16141 ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg
    16201 cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa
    16261 tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca
    16321 cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc
    16381 gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac
    16441 cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc
    16501 ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac
    16561 ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag
    16621 tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga
    16681 taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag
    16741 tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg
    16801 gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc
    16861 tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat
    16921 cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc
    16981 atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt
    17041 gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac
    17101 gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc
    17161 aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag
    17221 gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc
    17281 agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga
    17341 tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg
    17401 tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt agaagtataa
    17461 atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt
    17521 ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc
    17581 gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga
    17641 agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc
    17701 ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc
    17761 gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact
    17821 ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc
    17881 agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc
    17941 accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc
    18001 tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct
    18061 acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc
    18121 accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg
    18181 tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca
    18241 aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga
    18301 tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc
    18361 gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc
    18421 cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata
    18481 taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag
    18541 agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa
    18601 aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat
    18661 aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca
    18721 ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa
    18781 gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa
    18841 attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt
    18901 gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca
    18961 ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa
    19021 tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc
    19081 cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct
    19141 tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt
    19201 tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt
    19261 gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg
    19321 gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct
    19381 cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata
    19441 aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc
    19501 cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa
    19561 catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg
    19621 ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat
    19681 tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag
    19741 cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac cgattccgcc
    19801 cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg
    19861 catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa
    19921 gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga tccccaccaa
    19981 cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac
    20041 aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt
    20101 atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac
    20161 cagattcgac acgtaccagg caataacaaa acccacaaaa ccaaagtcaa aataccaggc
    20221 tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc
    20281 aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag
    20341 cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag
    20401 gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga
    20461 gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt
    20521 tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc
    20581 gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca
    20641 cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct
    20701 attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc
    20761 caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact
    20821 tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc
    20881 gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga
    20941 catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc
    21001 agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg
    21061 gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac
    21121 gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct
    21181 gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg
    21241 cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct
    21301 ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa ccaacggcaa
    21361 tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg
    21421 ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc
    21481 acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt
    21541 aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc
    21601 aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc
    21661 gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt
    21721 tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa
    21781 tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact
    21841 cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc
    21901 catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc
    21961 cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat
    22021 agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt acatgtgccg
    22081 ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt
    22141 cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca
    22201 gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg
    22261 caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc
    22321 cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt
    22381 attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa aacatattaa
    22441 ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca
    22501 cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt gtgtgaacat
    22561 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
    22621 ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca
    22681 attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc ggcgctggta
    22741 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
    22801 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
    22861 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
    22921 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
    22981 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
    23041 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
    23101 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
    23161 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
    23221 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
    23281 cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
    23341 gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg
    23401 catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc
    23461 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
    23521 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
    23581 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
    23641 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc acagcataat
    23701 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
    23761 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
    23821 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
    23881 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
    23941 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
    24001 caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa
    24061 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
    24121 gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
    24181 actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
    24241 ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac
    24301 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
    24361 gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
    24421 cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca gcaacaaatc
    24481 gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
    24541 aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat
    24601 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
    24661 aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt
    24721 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
    24781 acggcatggg caaagtgaaa tatcattttt ttaacgataa aaagagtgtt atttcttgtg
    24841 tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca ggtattccac
    24901 tggttaatat attcacatat gaaatgaata aatattggaa tatataaata ttgaatattt
    24961 tgattaatcc ccgtactaat tattcttcat aatcagtacg gggttgctac aacatgtatt
    25021 actttttcac cacaattaac ggttcaatat cactctcttt tttgatgacc agtgattcat
    25081 caccgcgcaa acacgtccca ccgtagttgc cgccaacggt gaaggtacat acctgaatgt
    25141 atttaccgtc cactttcggc aaacaccaca gttgctgata gatgtttttc tgctcggcaa
    25201 atttaccgct ggttttgtcc agcacctctt catgatggct gaccaggtcg atattgctac
    25261 cacagcgacc ggcgatcggt ttcactgcgt aacctgtttt caccaattca tcattaacag
    25321 tgaaatcggt atccagcagg taacgatggt gcgggaacag cgaccagagg atcggcagaa
    25381 tcgctttgtt gccggggatc accgtccaca gcggctcaaa gaccagcact tccgggcgca
    25441 gcaatacgtc gataagacgc acttcgtttt gcggatgacc ggtacggatt ggcaccgcag
    25501 caaactcacg gtcgctaact tcacgaatct gatcaaacgc ggtttcccac gcccaggttt
    25561 tccacacgca gttaaccagt cgcccttccc catcaatcag ttgcccggca gcatcccagc
    25621 ccagttcatc cagcccgcgc aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct
    25681 ccataaactg cgcgtgatag ttttcctcga tatctttgtc ctgcatgata tggacaaacg
    25741 gacgtgcacg actgtgtttc caggcaccag ccagttcgtt aatcagccct tccgccggat
    25801 tgaagccgtt gcctttatag ccctgctccg cccaacgttc gaggatcaag cccgcttcgg
    25861 tatgacagga ggcggagtcg gcgttgtatt cgtaaacctt caggccacgc tcatccatgc
    25921 agaaatccat acgaccagtg atcatatggt gacggcgacg ctgccaggag agacgcaaac
    25981 gtggccagag gattttcgga atgtcgaaca gcgccagcag gttgtcatct ttcagcacct
    26041 tgtcggttgc gtgaagatac atcagatgca gctcgttggt ggctttaatt agctcctgct
    26101 cggcactctc ggtaatggtg tagtaatgat acggatcctg attgatcacc tgaccgttgg
    26161 cctgcacata ggcgttttgt agcggatctt tttcatccag ccatttaccg tcaaactggc
    26221 ctttgttttc cagacgcgct ccactgattt tcagcagctc gcctgcaatt tccggctgcg
    26281 gtaagctgta ttcagtatct tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa
    26341 aagtgtcttt aagggtatag cagccgtttt cgaccaccat ctccagctcg cgcgtccact
    26401 gttgcccttg cggcaacggg gaatgaatca cgttctgttc cgcaatacgg actttgttgc
    26461 catgcaattg ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg cctttatccc
    26521 agataagaag cgcacccgcg accggcgcac gcggcgagcc gttaggaaat gcctgcaatg
    26581 gcaggatgtt gtcattaacc acttctcgca ggaagcgcag cgagaaaatc tcccacgcca
    26641 tacccacgtc agtaaagacc acaccgtaat tcagaaagag aaaacggcga gcaaattcaa
    26701 cgcattgcca cttgtggccc atatattcgt cgtcgatata gctacggaat acggcgtcat
    26761 cttcgtattc ctgcggatcg agagaactgt aatctgaaga gtagattgct accccacctg
    26821 gggcgtagcc caataatgtc ccgaacgggg catcctggct ggtcgttcct ttgctcatca
    26881 ctttaccttt aacaatacaa cctaagcagt tggcgtaatt gttgtgctct gattacctgc
    26941 tcagcgacat taaccggaca gaggtcagac taatcataca cctcatcgca gcggctggcg
    27001 caaagggtta aaaaattcac attctgtaca gcaagtgacc tgctacactg cttcaacact
    27061 accactcaga aggcaactca ctatgacaga caatacttat cagcccgcga aagtctggac
    27121 gtgggataaa tccgctggcg gcgcgttcgc caatatcaat cgcccgtttt ctggtccgac
    27181 gcatgaaaaa acgctgcccg ttggcaaaca tccattgcaa ctttattcgc tgggaacgcc
    27241 gaacggtcag aaagtaacga ttatgcttga ggagctactg gcgctgggcg ttactggtgc
    27301 agagtacgac gcctggctga ttcgtattgg cgatggcgat caattctcca gcggctttgt
    27361 cgaagtgaac ccaaactcga agatcccggc gctgcgcgat catacgcata atccgccgat
    27421 ccgcgtgttt gaatctggtt cgatcctgct ttatctggcg gagaaatttg gctacttcct
    27481 gccgcaggat ctggcaaaac ggactgaaac gttgaactgg ctgttctggt tacagggtgc
    27541 cgcgccgttc ctcggcggtg gttttggtca cttttaccat tacgcgccgg tgaaaattga
    27601 gtacgccatc aaccgcttta ccatggaagc caagcgcctg ctcgacgtgc tggataagca
    27661 actggcgcag cataagtttg ttgcgggcga tgagtacacc attgcggata tggcgatttg
    27721 gccgtggttt ggcaacgtgg tgctcggcgg cgtgtatgat gccgcagagt ttctggatgc
    27781 gggaagttat aaacatgtgc aacgctgggc gaaagaagtg ggcgaacgtc cggcagtgaa
    27841 acgtgggcgt attgttaacc gcaccaacgg gccgctgaac gagcagttgc atgagcgcca
    27901 tgacgccagt gattttgaga caaatacaga agataagcgt caggggtaag ggttggtgtt
    27961 cgtcgcagca agccatccag gccggataag gcgttcacgc cgcatccggc aatcgtgcac
    28021 aatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaagctaatc atcacgcgct
    28081 ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc
    28141 gatgatgctc atggcaaatc cgacgtgtac cagcacccac tggcccagca gatcggcagg
    28201 gttaccttcg caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg
    28261 cgcaagctgg tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat
    28321 tgttaactcc ggttatttcg attgccggac aaacgcgaaa aagaaccttt agcgggtgtt
    28381 ccctctcacc ctaaccctct ccccggaggg gcgaggggac cgtccgtgcg ctttttaggc
    28441 gttgtcctca gtcttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga
    28501 cacgcaaccg ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca
    28561 cctggctgca atcccagcac caggcctggg cgggtttata gacgatatgt aaatcgcacc
    28621 cttgcgccac cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa
    28681 cgcaggagag cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct
    28741 ccgcctgccg ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg
    28801 cccctcctgc ggccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattggtg
    28861 actggcaacg aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ttcttcaatc
    28921 gacatgctgt gcgaaagcgg cgacatcagc gaacaggaga ggtattgcga aacaccgtcc
    28981 agttcgccaa cggtaaaggt catggtgcca tacggcagtt gcagaccaat tttttcactc
    29041 actttgcgca gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac
    29101 ggggtgatca cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc
    29161 ggcattgaag gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac
    29221 gctgcctgta cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat
    29281 cgcctcccgt gggatggctt ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag
    29341 cgcaggttca atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc
    29401 cgggatcacg ccgaccaggg tcagcttttt cggaaactca ccggtgaagc gcagggccga
    29461 caatacgtcg gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac
    29521 ttcttcatcc cgcaggatca tcatcgttcc cggagtgttc tttttcgaca caatggcatc
    29581 cgcaataatc agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc
    29641 gccatcgagg atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat
    29701 ccgcacgccg atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg
    29761 cattacagaa ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca
    29821 caggccatgc acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga
    29881 tcggcaaccg gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta
    29941 cgcggaccag agttccaggt tgatggaaca accgcctggt agttgctgat gataccgtct
    30001 ttaataacca tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg
    30061 aactcacccg ttgccggaat gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata
    30121 ttggtgatca gtgcactgta ttggttttgc aggatatcct gcaattcaca gcagtgaacg
    30181 gtacgaccaa taatacggcc cagcgtggag tgcagctgtg ccacttccag cgtgttgcca
    30241 gtcagtttct gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttcgcgacct
    30301 gccgccagtt tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa
    30361 gtcggtgatt tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg
    30421 gtggtacctt cccacggcgc ctgtggcgct tcgtctttat accaggagtg cttcgcgctt
    30481 tcctgaatgc ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac
    30541 agatccgcat tctcaatgta gcctcccggg aacaggaagc tgccgttttt gctgtcggtc
    30601 gggaattccg gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg
    30661 tagaacgcgg cgataaccgc ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt
    30721 ttgtcgatga aagacttgat gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg
    30781 aggttgattg ggttcgcgac accacccaca gccaggttct gaatgtgcgg cgttttaccg
    30841 cccagcagcg ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc
    30901 gctaccgcaa tcaggttcac ttccggcggc agtttcatcg ccgggtgacc ccagtagcca
    30961 ttggcgaaaa tacccaactg accgctggca accagatctt tgatcttgtt ctgaacttta
    31021 gtgaactctt ccggactgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct
    31081 ttggttgggt cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga
    31141 tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat
    31201 tgcgcgttaa ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc
    31261 gcgtgagtgg tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga
    31321 tcgcggtttt tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc
    31381 gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc
    31441 ggatcaatag taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt
    31501 gttttaaagg aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa
    31561 tagccacaaa accaatagaa atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc
    31621 cgcccgggtt gaatgccacc aacgaatagg tcagacgcca ggttgcacaa cctaacagtg
    31681 cgctcagtgc tgacaggaac agcatacggg aatcattacg cagcttcgcc acacgcagaa
    31741 cgaccagcgg gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac
    31801 cggcaaacgc taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcccgatga
    31861 aaatcgccag caacacactg atggtgttgg tcagcttgac gaacaggctc ttttcatccg
    31921 gaccgttgcc acgcagaccc gcctgcacca gtgaaccttc aaagatgaca atcgagaagc
    31981 ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccagagcg
    32041 gatgcacctt gtagcccgcc gagatcatca gcgaccccat tgaagactgg tgcatggtcg
    32101 gcagcagcgc accgagcgcg atgatgaaga acatcacctt gttcagacgt tgtagcgaca
    32161 ccttccagcc cagacgttca aacagtgccg gagcaaactc cagagccatc acgccgatgt
    32221 agatggtcat acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa
    32281 tgtagaagta cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca
    32341 gtgagtaacc aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac
    32401 ggttaaagac gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtgccaa
    32461 tcagcaggtc aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag
    32521 agcccagacc gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc
    32581 caaaaatcat gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca
    32641 tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag
    32701 cccgccagca cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg
    32761 gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca
    32821 cccgtcagta ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa
    32881 tatttcggca ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg
    32941 tattcgctgc caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc
    33001 gtaccgaaaa tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta
    33061 tcgagacgtt ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg
    33121 ttgttgtagt cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg
    33181 gtgcacacgt ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag
    33241 accgggcaca cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac
    33301 gcgtagccgt tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg
    33361 atgttattgg tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg
    33421 ttacgttcag ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac
    33481 aaggtcgagt cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc
    33541 gcatgactga cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata
    33601 aaattacgtc tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac
    33661 gacccagttc acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga
    33721 gcaaaccaat agcgcctgca gagacgttgc cgccctcttt agcgttaaca tccggtttct
    33781 gtgaacgcgg agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga
    33841 tacctttttc gttacagcca tagcacgggt gaccaatcgc caccggccac acaccgccaa
    33901 catcgcagaa ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt
    33961 ggtacaagca ccagccttcg cggtggcctt catcaccgaa ctctttggca aaacgaccag
    34021 catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg
    34081 gacggttttt gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg
    34141 cgaggaagtt gtgcgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca
    34201 gaacttcttg caagctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc
    34261 acgcggagca ggaaccaata gcgataatgg ctgctgagcc ttccgccgct ttgcggatgt
    34321 gatccacaat cggctcaccg gcaaccatgc aataaatacc gttatctttt aatgggatgg
    34381 aaccatccac cactaacaca tactgccctt tgtacttctc gagagcgtta tgtttgttct
    34441 cttcgacctg atgaccgaag gcggcggaaa gcacttcgtg atattccaga gagatagtct
    34501 ccagcacgag gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg
    34561 tgcactcctg cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg
    34621 ccatctctgc agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct
    34681 tcatgaaatc acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt
    34741 atagttattc cgttgcgaga acctggcata tattttgcct caatcgcaaa atcaataatg
    34801 cgatcgatgc gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg
    34861 gggcgctaaa acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat
    34921 gtttgatcaa ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt
    34981 attctcacga aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat
    35041 acccgcttcc caggcgctaa gctggcgatc tccggtcttt tcgtacatcc tgtgtgagta
    35101 gcctaataca aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc
    35161 ggcatgcgtt gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca
    35221 cctcattcat tatatatcgc catatataac gatattttag gcaaagaacg acttttgcgc
    35281 acgctatttt ttcatacttg tttaagacgt aatcccgtaa gagataaacc tatcaggaga
    35341 gatatttatc gaaccatttc agtgtccttt gccaggcaag atcggcggca gatttgtcat
    35401 aacggggcgt ggaatcatta tggaatccgt gattaacccc cggatagata taagcctcat
    35461 aaaccttatt attgtctttc aacgccgcct cgtaaggagg ccagccctcg ttgattcggg
    35521 tgtccagttc cgcgaagtgg agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg
    35581 gtgcctgacg accataaaac ggcaccgcgc aggccagttc cggatacgcg acagccgccg
    35641 cgttcgatac gccaccgcca tagcaaaatc cggtaatacc cactttacct gtcgcttgcg
    35701 gatagcgttg cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa
    35761 cctgctgttg cagctcacga cctttatcat catttcccgg ataacctcca acggaactta
    35821 atccgtcagg tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga
    35881 tatacggatt cagtccacga ttctcatgca ccacccccac ggctggcgtt ttgccgctca
    35941 tttttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg
    36001 tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat
    36061 ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca tacttcgccg
    36121 caaggttgag gaactcacgt ttcgaaattt tcccgtgaac gtaatagtcg tagtaatcca
    36181 acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcattc ctcaactggt
    36241 gtttttttaa gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc
    36301 agactacggg atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg
    36361 gtggaaagga gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta
    36421 ccgctattgc ggaaaaagtg gtttacgcct gcccgcatta tcgctcggtt tatggcacaa
    36481 tttcggtcac gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt
    36541 aggcattacg cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga
    36601 gaactttggt cgcctgctgc gcgaggattt tgctgcttat cgcgatgaac tgattatctc
    36661 taccaaggct ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata
    36721 cctgctcgcc agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt
    36781 ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca
    36841 tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac
    36901 gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc
    36961 gtacaattta ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa
    37021 cggcgtgggc tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct
    37081 caacggcatt ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac
    37141 gccgaaaatg cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca
    37201 gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt
    37261 gacgtcggta ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct
    37321 gaataatctg acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg
    37381 cgagctgaat ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa
    37441 ccgcgcccga atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa
    37501 atgcagaaag cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga
    37561 catttttcgc atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg
    37621 ctgctacttt gtttttcagc gacgttgcgt ccattttccc cagccacttc agcttctctt
    37681 tgttctcgga gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca
    37741 ccagcaaacc gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg
    37801 attccgccat cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg
    37861 ccagggcaac taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag
    37921 catacattgc attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca
    37981 acagtatatc gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct
    38041 ttgtcgcata attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc
    38101 gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc
    38161 cgaccacgca gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaagcatcc
    38221 aggcgaagat gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc
    38281 tgaaagatcg taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca
    38341 tcgcttacgc gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag
    38401 acgctcagga tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag
    38461 gcgatttaag cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag
    38521 gcgggctgga tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag
    38581 acctcaccag cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc
    38641 taacccagga agcgatcccc ctgctaccga aaggtgcaag tgtcatcacc acttcgtcaa
    38701 tccaggcata ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc
    38761 tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg
    38821 tcgcgccagg ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga
    38881 tcccgcagtt tggtcagaaa acgccgatga agcgtgcggg gcaaccggcg gaactggccc
    38941 ctgtatatgt ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg
    39001 tgtgcggcgg cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc
    39061 attgtgaatt cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga
    39121 agattcaaca tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga
    39181 gcacctaatg ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca
    39241 acatgttgca atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta
    39301 cttcgctttg gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag
    39361 cgtatccatt accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat
    39421 ggtggtgtct ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt
    39481 gaccggatcg ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt
    39541 ttccgggcgc ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt
    39601 cgctaacggt gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg
    39661 cgtcacgttg atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc
    39721 catcggacat tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct
    39781 ggcttccaga tccaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag
    39841 catcgcttta aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat
    39901 cgctgccacg aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac
    39961 tgccaggtta gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac
    40021 cgtaccaaac agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc
    40081 gcggcccatt tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc
    40141 tttaatacct tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt
    40201 gagcaaatgc aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt
    40261 taaggaacgt gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa
    40321 gaactctacg ctcttactga agaagattgc ccaggtgact acagaggcca aaataagccc
    40381 aatcatcacg cacttaacga caatatcggc gtactgatac ataccccaga cggaaaggtc
    40441 cgtctgcatt aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc
    40501 ataataatat caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc
    40561 gtacctttgc tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg
    40621 cgaaaatttt ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt
    40681 tagacatcca gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc
    40741 aactggtgaa tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc
    40801 agcgcgcttc ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc
    40861 gcgccaatgg cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac
    40921 aagcgatgtg tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag
    40981 cggttgctaa ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca
    41041 ccgcctatga accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga
    41101 catcgtggtt tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta
    41161 aaatcgtgtt tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga
    41221 ttgttgccgc cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg
    41281 cagccggtgt gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca
    41341 ccaaatatct ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt
    41401 gctgggagca gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg
    41461 cctatataac cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa
    41521 gcagtctgaa agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc
    41581 ctgccctgcc tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg
    41641 ggctattttc ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg
    41701 ataacttcag tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg
    41761 caaatcaacc agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga
    41821 ccttgattcg cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg
    41881 ccggttttgc acggattgta taacattgcc acttttggac aattttgcag acattttatt
    41941 gtgaaaagtc ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca
    42001 ataggcagat aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat
    42061 atcatcgctg cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc
    42121 gttgtttact ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca
    42181 tttttgccag gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg
    42241 gattttctgc ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt
    42301 tatattcagg ggagctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt
    42361 cctgctaaat atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg
    42421 ctggctggac gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc
    42481 ggtctgccaa accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc
    42541 gtggtaacca gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat
    42601 caggtaatga cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc
    42661 acgctgtttg tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg
    42721 gctgctgtac cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc
    42781 ccccggcgtc acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata
    42841 gccgacgcgc atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc
    42901 cttatgcaga cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa
    42961 attatggtgg aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag
    43021 gttttcggtg tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt
    43081 ctggcgactg accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag
    43141 aatttcgcgg atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc
    43201 gagtaaccgc tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct
    43261 ggctgccatc gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa
    43321 ctgcaaaata tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt
    43381 ttcgcactca aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa
    43441 cacgcgttca ttgatataac cgattttatg cccggaaaag agaattatga tgccaggctc
    43501 gtacatcacc ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa
    43561 cagttctgac aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg
    43621 acaaatctct tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg
    43681 tgcgcaattt tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa
    43741 tgagcattga gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc
    43801 tgccctcata ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc
    43861 aacccgcatt ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga
    43921 tgctcgcgta ttgattacct acggtggcgg cagcgtgaaa aaaaccggcg ttctcgatca
    43981 agttctggat gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc
    44041 ggcttatgaa acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct
    44101 gctggcggtt ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa
    44161 ctatccggaa aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag
    44221 cgccatcccg atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg
    44281 cgcggtgatc tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca
    44341 gccggtattt gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc
    44401 taacggcgta gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga
    44461 tgccaaaatt caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc
    44521 gaaagccctg aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac
    44581 tcaggcgctg aacggtttga ttggcactgg cgtaccgcag gactgggcaa cgcatatgct
    44641 gggccacgaa ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct
    44701 gcctgcactg tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga
    44761 acgcgtctgg aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc
    44821 aacccgcaat ttctttgagc aattaggcgt gccgacccac ctctccgact acggtctgga
    44881 cggcagctcc atcccggctt tgctgaaaaa actggaagag cacggcatga cccaactggg
    44941 cgaaaatcat gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc
    45001 tttttacgcc tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct
    45061 caccggagcc tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt
    45121 attaagctac aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt
    45181 aatgaggaag taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat
    45241 accgccgcgg cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc
    45301 aacagagaag aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc
    45361 gaagccctgc tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg
    45421 cactggcccg ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg
    45481 caaaaagagg gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa
    45541 cgcctgattg atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg
    45601 atgcaacaac gccagctaca cgcttggaac gcgacacaca aaatccagac cgaatcctgg
    45661 agcccattag cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca
    45721 gataaatacg gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg
    45781 gtggtgatcc cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat
    45841 ttccgtctcg acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc
    45901 ggtcccgatc ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt
    45961 ccgctaccgg acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt
    46021 ccttgttcag gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag
    46081 aacaactcat ctatatccgg gataaacgca acggagaggt gaaaaacaga tgaaaataat
    46141 acttctgttt ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac
    46201 cgtagaacaa acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta
    46261 tttcggtgaa accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct
    46321 gaacgacaat cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga
    46381 ttgtcaggat tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga
    46441 tcatgccgat gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca
    46501 gacactgaaa atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca
    46561 tggcagcgtc ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc
    46621 gttgcagaaa gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta
    46681 tagctggccg tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta
    46741 taaaaccacc ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt
    46801 tatttacgac aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat
    46861 tcgagtcctg gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa
    46921 tggcaacaat gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc
    46981 tgattttatc cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc
    47041 cagattaaag caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa
    47101 cactatatga aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt
    47161 ggctgcgtca atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc
    47221 aaaatcgccc ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa
    47281 tccgaagcta acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc
    47341 tgcttgcgta tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga
    47401 ttttttttaa cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg
    47461 agattgcgta gacgacgctt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg
    47521 cgtatgttta gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc
    47581 ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc
    47641 catcgcttcc agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa
    47701 cgctttatgc aaacgacgct gtttgtcgcc cttcggtacg aagacgcctt cgctcttata
    47761 accaatcttc gccagcggtt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg
    47821 cgatgggtag aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc
    47881 caaattcacc atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag
    47941 atactgctct ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata
    48001 gctgcccatg cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc
    48061 aatcttcaga taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc
    48121 cacggcgatg tcataacgta cgcctgaggc gataaggatt tttttaatgc ctttcagatc
    48181 acgcgcacgg cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat
    48241 atccggataa acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg
    48301 caacatatac atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg
    48361 aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat
    48421 aatgcgccct tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat
    48481 aatgttgacc gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg
    48541 atgtggcacg cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg
    48601 gatagcaggc gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc
    48661 gcagcctggg ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc
    48721 gcccttcact ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg
    48781 cggtggctgc acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc
    48841 cgcacacggc aaatcttcgc catacggatg cgggattggg tcgattttcc ctagggtatc
    48901 aagacgggtg gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt
    48961 attacgcaca tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc
    49021 caccagcgga cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac
    49081 ggaacggcgc acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc
    49141 aataccgccg aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac
    49201 cagcgtggcg cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg
    49261 acgtaaacgg cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac
    49321 accaaaaaac agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg
    49381 ctgggcgatg atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat
    49441 cccgaagctt gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc
    49501 ccagccaagt tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca
    49561 ggcggcccag tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga
    49621 gctcattttg cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga
    49681 acgaaagaga aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac
    49741 ggtaccggat tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga
    49801 ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc
    49861 acttgtccgt caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg
    49921 ccattcacgt tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc
    49981 gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg
    50041 tcccacagct gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt
    50101 ggcgaaccag ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc
    50161 ggcagaaggc cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc
    50221 tcaaagaagc cgcgaatacg atcaacaatg ctcgccgctt caccacaggt gatcgacact
    50281 tcatcgccgt tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt
    50341 tgcttcaccg atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg
    50401 cgaccatcgc tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag
    50461 cgcacccagc cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta
    50521 tcaccaacaa agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc
    50581 cgtttatcct ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc
    50641 gacttgctga cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga
    50701 gccgtgcggt tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc
    50761 agtacgggtg cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg
    50821 cctggtacct gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa
    50881 ataagcttaa catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg
    50941 attccccaga ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc
    51001 ataaacagcg gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt
    51061 tgcccggctg cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc
    51121 tgaatgaact gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat
    51181 accgtgcagg aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg
    51241 cttcgcgttc tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac
    51301 aatgggcagc cagctcacga acctgatctt tgccatactg actgacgtca attggcggca
    51361 gcatttcgac aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg
    51421 tagagacgca cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag
    51481 tcttgaacgg tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg
    51541 aaatacggcg ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc
    51601 gattgtttct gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga
    51661 tccacagcaa gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg
    51721 tcaccatgtc atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag
    51781 catctgcagg tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca
    51841 tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc
    51901 cgaatacaca gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca
    51961 taacaccctc tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc
    52021 attgttaaaa gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac
    52081 ggtttcaagg gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa
    52141 cgtttcatcc ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc
    52201 ttcgctatca ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg
    52261 caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc
    52321 cggacgcagt ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag
    52381 aacgtacagt tgcgccagac catcctcgcc acgcgcggct tctgccgatg gaatgttgat
    52441 aatcttgttg cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat
    52501 acggcctgcc tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg
    52561 cataacatgg gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgctac
    52621 cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg
    52681 atcgtcgctt tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc
    52741 ggtgagtggt tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct
    52801 acgaccggtg gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt
    52861 gaagctatca cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct
    52921 gcgtacccag cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg
    52981 ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta
    53041 ggcttgcgcg tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc
    53101 caaaatgccc tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt
    53161 catctcttcc agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt
    53221 ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat
    53281 ctcaatcact tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg
    53341 cttgaggact ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac
    53401 cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat
    53461 catattgagg ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat
    53521 cacctgatcc atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc
    53581 gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc
    53641 agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac
    53701 cgcgccatct tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta
    53761 gattttacga atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc
    53821 ctgcacgata tccagcagct gatcgagcgt agttttcggc tggtcgatta atgcgattgc
    53881 cgcctgagct acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat
    53941 accggtggtg ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg
    54001 caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag
    54061 cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg
    54121 atcgtccggc gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg
    54181 ctgcgccatc aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc
    54241 cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag
    54301 ttcagacatc gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg
    54361 caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc
    54421 atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc
    54481 agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg
    54541 agatcagcgt gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc
    54601 ttgatataca actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc
    54661 catgccacac cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc
    54721 gagtccaggg aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt
    54781 ttagctgtgt ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat
    54841 atccccgaga cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt
    54901 accggagaaa ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc
    54961 gcgagtgatt tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca
    55021 tccgtttcca gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc
    55081 ggtgctttac gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc
    55141 gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta
    55201 agttcaccat tgctcacacc ataacgatta tcgggaatca gttcgctaac gctgccgcag
    55261 atatcaaagc gaaacgcttc cggtgcggtg gtggcagggg cgtcccaggc gacgccaaaa
    55321 gtctggcttt ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc
    55381 acactgtagt tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat
    55441 gggaaatcga taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt
    55501 attcccggga acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt
    55561 gacgtcaatt tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc
    55621 tttcggcgtc accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc
    55681 gctggcatct ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag
    55741 ggttacccag gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt
    55801 cggcccctga aatcctccgg cctgactttg cgttaccgat gggccagaaa aaccgccctg
    55861 ctctgctgcc atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt
    55921 tttcatgttt attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa
    55981 caacttctta agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt
    56041 tactggcagc aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga
    56101 tgacatgctg attggtgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga
    56161 ctggtttaca caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt
    56221 gatcctggat ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga
    56281 gaaaggtcag cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt
    56341 agaagggctg cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt
    56401 cgccgccagg ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg
    56461 ccacggtaac gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt
    56521 aacactgaaa ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt
    56581 actgccgcgc aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa
    56641 tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac
    56701 cgtgcatggt attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg
    56761 cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc
    56821 tttgtcgcct ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg
    56881 ctgtttgcca aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg
    56941 gcacagacgc caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc
    57001 atctttaccc acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc
    57061 tatagctatc aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg
    57121 cgttttgtct ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg
    57181 gaataccgtg aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc
    57241 gcactgccga ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg
    57301 aacaaactgg cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg
    57361 actggcgtac ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc
    57421 acacatgcga tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt
    57481 agcccgttaa cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg
    57541 caggcgcgga aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg
    57601 gttgatcaac tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg
    57661 gagatcccgc ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag
    57721 caggcgaaaa ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa
    57781 ccgctattgc taagtttgtt ggtgcgaaat ttgctggata acggccccgg tgtgacacca
    57841 gaggcactgg cgcgaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc
    57901 agtggacttg ggctatcgat tgtccagcga atcgccaagc tgcatgacat gaatgttgag
    57961 tttgggaatg cggaacaagg tggatttgag gcgaaggtaa gctggtaact tctctgtcta
    58021 tgagagccgt taaaacgact ctcatagatt ttactaatag caaaatataa accgtcccca
    58081 aaaaagccac caaccacaaa ccagacaaat aacgagcgag aatacatata gccaaaactc
    58141 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa
    58201 aaatgctgca atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca
    58261 aatattgcct cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagaatctat
    58321 taaccaataa gtaagcatta tatatagcaa tgcttcaaat attgttttaa ttttctgctt
    58381 taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata
    58441 ttttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg
    58501 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
    58561 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
    58621 aaagactttg cacattttgc taatttcaca gtaccgctct gtgacgtact atagtcgaca
    58681 aacgtctcac cttgagatta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
    58741 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
    58801 cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg
    58861 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
    58921 ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcacgggact
    58981 ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc
    59041 ctggtacagg acaaaaaata tatgctttct ctaacctgga acgcaccaat ggaagccttc
    59101 accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat
    59161 aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata
    59221 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
    59281 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
    59341 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
    59401 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct
    59461 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
    59521 atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta
    59581 aaaggcgacg tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt
    59641 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
    59701 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
    59761 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
    59821 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
    59881 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
    59941 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
    60001 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
    60061 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
    60121 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
    60181 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
    60241 atcttacctt tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat
    60301 ccgcctgcgg agtcaccttc cacgaggaac agttcggtac ggttaaggcc ctgcgcggta
    60361 caatcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt
    60421 ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc
    60481 gcctgaacgt tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat
    60541 gccgcacact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
    60601 attttaaccg acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg
    60661 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
    60721 cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg
    60781 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
    60841 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
    60901 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
    60961 tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag cacatgcgtc
    61021 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
    61081 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
    61141 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
    61201 ttcgacaagg cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
    61261 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
    61321 actccttctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac
    61381 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
    61441 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
    61501 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
    61561 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
    61621 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
    61681 tatgacagtc tggcggcagg aagcatagta cgccaccgcc tggcggtaat ccagcacttc
    61741 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
    61801 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
    61861 ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac
    61921 cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga
    61981 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc
    62041 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
    62101 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
    62161 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
    62221 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
    62281 cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
    62341 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
    62401 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
    62461 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
    62521 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
    62581 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
    62641 tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg
    62701 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
    62761 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
    62821 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
    62881 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
    62941 gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag ccagaggaag ggttaacagg
    63001 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
    63061 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
    63121 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
    63181 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
    63241 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
    63301 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
    63361 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
    63421 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
    63481 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
    63541 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
    63601 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
    63661 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
    63721 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
    63781 ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac
    63841 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
    63901 aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg
    63961 ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
    64021 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
    64081 agcattgtta atgtcctagc actaatagtg aattaaatgt gaatttcagc gacgtttgac
    64141 tgccgtttga gcagtcatat gttaaattga ggcacattaa cgccctatgg cacgtaacgc
    64201 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
    64261 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
    64321 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
    64381 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
    64441 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
    64501 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc
    64561 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
    64621 aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
    64681 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
    64741 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
    64801 tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
    64861 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
    64921 actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
    64981 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
    65041 gccaggacct ggagcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg
    65101 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
    65161 ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct
    65221 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
    65281 ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt
    65341 cctccttcaa caacattaat gcttctatca gtagtattaa cgcctacaaa caagccgtag
    65401 tttccgcgca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
    65461 ttgttgatgt gttggatgcg accaccacgc tgtacaacgc caagcaagag ctggcgaatg
    65521 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg
    65581 agcaggattt gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
    65641 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
    65701 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
    65761 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
    65821 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
    65881 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
    65941 gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg
    66001 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
    66061 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
    66121 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
    66181 gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc agcccaggct ggcatggcac
    66241 cagaaaacca ggcgcaggct cagcaatcca gcgggagttt ctggatgccg ctgatggccg
    66301 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
    66361 cctcgaaaaa cccggccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
    66421 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac
    66481 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
    66541 ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga
    66601 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
    66661 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
    66721 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
    66781 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac
    66841 ctggagtttt gtgcgtcaat catggctgac tcaccagcca tcgctttatt cgcgtcttga
    66901 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
    66961 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
    67021 taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
    67081 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
    67141 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc
    67201 caccgagttc ctctacatcg acgagatagg tttaggcgaa aaaggccagt tcacggattt
    67261 acaggatcag gtgatttcta acctgttcaa gctttatccg tgggaattta tgctgcgcga
    67321 aatgttctcc accaagctgg aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag
    67381 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
    67441 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
    67501 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga
    67561 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
    67621 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg
    67681 aattggcatt cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca
    67741 tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct
    67801 atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt
    67861 ggctcctgcc catcccacac acctaacaca tacaacaacg gcagatagtg ctctggcgtt
    67921 gggttcaata acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc
    67981 ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac
    68041 ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg
    68101 accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag
    68161 gcggcaggtt tgctactgtc gatactcaac tgcaccatag ggatatcggc gtcaggatac
    68221 atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc
    68281 accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca
    68341 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    68401 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    68461 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    68521 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    68581 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    68641 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    68701 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    68761 gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc gaaccgctta
    68821 ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    68881 cttcctgccg cactagcagc tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc
    68941 ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca tccgcaggat
    69001 ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca agcgcacagc cattctgctc
    69061 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    69121 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa
    69181 ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg
    69241 tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    69301 ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    69361 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    69421 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    69481 acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc
    69541 agcttcacga aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta
    69601 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt
    69661 caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat
    69721 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    69781 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    69841 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    69901 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    69961 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    70021 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    70081 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
    70141 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    70201 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    70261 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
    70321 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    70381 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    70441 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    70501 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    70561 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    70621 gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag
    70681 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
    70741 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
    70801 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
    70861 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagc
//
LOCUS       DAJVPT010000004        70481 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVPT010000004 DAJVPT010000000
VERSION     DAJVPT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..784
                     /locus_tag="OQM47_000251"
     CDS             <1..784
                     /locus_tag="OQM47_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1478542.1"
                     /translation="SLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNN
                     HNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALID
                     ANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVP
                     NRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGI
                     SSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            795..1886
                     /locus_tag="OQM47_000252"
     CDS             795..1886
                     /locus_tag="OQM47_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1478543.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1929..2789)
                     /gene="rsmI"
                     /locus_tag="OQM47_000253"
     CDS             complement(1929..2789)
                     /gene="rsmI"
                     /locus_tag="OQM47_000253"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1478544.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2854..4890
                     /gene="lpoA"
                     /locus_tag="OQM47_000254"
     CDS             2854..4890
                     /gene="lpoA"
                     /locus_tag="OQM47_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1478545.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4848..5243
                     /locus_tag="OQM47_000255"
     CDS             4848..5243
                     /locus_tag="OQM47_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1478546.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5263..5853
                     /gene="diaA"
                     /locus_tag="OQM47_000256"
     CDS             5263..5853
                     /gene="diaA"
                     /locus_tag="OQM47_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1478547.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5863..6438
                     /gene="yraP"
                     /locus_tag="OQM47_000257"
     CDS             5863..6438
                     /gene="yraP"
                     /locus_tag="OQM47_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1478548.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6552..7592)
                     /locus_tag="OQM47_000258"
     CDS             complement(6552..7592)
                     /locus_tag="OQM47_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1478549.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVSGLALLF"
     gene            complement(7665..8300)
                     /locus_tag="OQM47_000259"
     CDS             complement(7665..8300)
                     /locus_tag="OQM47_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1478550.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8428..8946
                     /gene="yhbO"
                     /locus_tag="OQM47_000260"
     CDS             8428..8946
                     /gene="yhbO"
                     /locus_tag="OQM47_000260"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1478551.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8926..9369)
                     /locus_tag="OQM47_000261"
     CDS             complement(8926..9369)
                     /locus_tag="OQM47_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1478552.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9420..9722
                     /gene="yhbQ"
                     /locus_tag="OQM47_000262"
     CDS             9420..9722
                     /gene="yhbQ"
                     /locus_tag="OQM47_000262"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1478553.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9709..10212)
                     /locus_tag="OQM47_000263"
     CDS             complement(9709..10212)
                     /locus_tag="OQM47_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1478554.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10206..10730)
                     /locus_tag="OQM47_000264"
     CDS             complement(10206..10730)
                     /locus_tag="OQM47_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1478555.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10939..11934
                     /locus_tag="OQM47_000265"
     CDS             10939..11934
                     /locus_tag="OQM47_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1478556.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11943..12821
                     /locus_tag="OQM47_000266"
     CDS             11943..12821
                     /locus_tag="OQM47_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1478557.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12902..13909
                     /locus_tag="OQM47_000267"
     CDS             12902..13909
                     /locus_tag="OQM47_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1478558.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14027..15271)
                     /gene="mtr"
                     /locus_tag="OQM47_000268"
     CDS             complement(14027..15271)
                     /gene="mtr"
                     /locus_tag="OQM47_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1478559.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15425..17314)
                     /gene="deaD"
                     /locus_tag="OQM47_000269"
     CDS             complement(15425..17314)
                     /gene="deaD"
                     /locus_tag="OQM47_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1478560.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17307..17387)
                     /gene="yrbN"
                     /locus_tag="OQM47_000270"
     CDS             complement(17307..17387)
                     /gene="yrbN"
                     /locus_tag="OQM47_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1478561.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17494..18378)
                     /gene="nlpI"
                     /locus_tag="OQM47_000271"
     CDS             complement(17494..18378)
                     /gene="nlpI"
                     /locus_tag="OQM47_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1478562.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18487..20622)
                     /gene="pnp"
                     /locus_tag="OQM47_000272"
     CDS             complement(18487..20622)
                     /gene="pnp"
                     /locus_tag="OQM47_000272"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1478563.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20869..21138)
                     /gene="rpsO"
                     /locus_tag="OQM47_000273"
     CDS             complement(20869..21138)
                     /gene="rpsO"
                     /locus_tag="OQM47_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1478564.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21287..22231)
                     /gene="truB"
                     /locus_tag="OQM47_000274"
     CDS             complement(21287..22231)
                     /gene="truB"
                     /locus_tag="OQM47_000274"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1478565.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22231..22632)
                     /gene="rbfA"
                     /locus_tag="OQM47_000275"
     CDS             complement(22231..22632)
                     /gene="rbfA"
                     /locus_tag="OQM47_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1478566.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22796..25444)
                     /gene="infB"
                     /locus_tag="OQM47_000276"
     CDS             complement(22796..25444)
                     /gene="infB"
                     /locus_tag="OQM47_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1478567.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25469..26956)
                     /gene="nusA"
                     /locus_tag="OQM47_000277"
     CDS             complement(25469..26956)
                     /gene="nusA"
                     /locus_tag="OQM47_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1478568.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26984..27436)
                     /gene="rimP"
                     /locus_tag="OQM47_000278"
     CDS             complement(26984..27436)
                     /gene="rimP"
                     /locus_tag="OQM47_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1478569.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27643..27719)
                     /locus_tag="OQM47_000279"
     tRNA            complement(27643..27719)
                     /locus_tag="OQM47_000279"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27683..27685),aa:Met,seq:cat)
     gene            28067..29410
                     /gene="argG"
                     /locus_tag="OQM47_000280"
     CDS             28067..29410
                     /gene="argG"
                     /locus_tag="OQM47_000280"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1478570.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29418..31025)
                     /locus_tag="OQM47_000281"
                     /pseudo
     CDS             complement(29418..31025)
                     /locus_tag="OQM47_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31503..31589)
                     /locus_tag="OQM47_000282"
     tRNA            complement(31503..31589)
                     /locus_tag="OQM47_000282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31553..31555),aa:Leu,seq:gag)
     gene            complement(31604..31936)
                     /gene="secG"
                     /locus_tag="OQM47_000283"
     CDS             complement(31604..31936)
                     /gene="secG"
                     /locus_tag="OQM47_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1478571.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32164..33501)
                     /gene="glmM"
                     /locus_tag="OQM47_000284"
     CDS             complement(32164..33501)
                     /gene="glmM"
                     /locus_tag="OQM47_000284"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1478572.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33494..34342)
                     /gene="folP"
                     /locus_tag="OQM47_000285"
     CDS             complement(33494..34342)
                     /gene="folP"
                     /locus_tag="OQM47_000285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1478573.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34432..36366)
                     /gene="ftsH"
                     /locus_tag="OQM47_000286"
     CDS             complement(34432..36366)
                     /gene="ftsH"
                     /locus_tag="OQM47_000286"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1478574.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36466..37095)
                     /gene="rlmE"
                     /locus_tag="OQM47_000287"
     CDS             complement(36466..37095)
                     /gene="rlmE"
                     /locus_tag="OQM47_000287"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1478575.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37221..37514
                     /gene="yhbY"
                     /locus_tag="OQM47_000288"
     CDS             37221..37514
                     /gene="yhbY"
                     /locus_tag="OQM47_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1478576.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37670..38146)
                     /gene="greA"
                     /locus_tag="OQM47_000289"
     CDS             complement(37670..38146)
                     /gene="greA"
                     /locus_tag="OQM47_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1478577.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38394..39827
                     /gene="dacB"
                     /locus_tag="OQM47_000290"
     CDS             38394..39827
                     /gene="dacB"
                     /locus_tag="OQM47_000290"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1478578.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40013..41185)
                     /gene="cgtA"
                     /locus_tag="OQM47_000291"
     CDS             complement(40013..41185)
                     /gene="cgtA"
                     /locus_tag="OQM47_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1478579.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41201..42166)
                     /locus_tag="OQM47_000292"
     CDS             complement(41201..42166)
                     /locus_tag="OQM47_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1478580.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42293..42550)
                     /gene="rpmA"
                     /locus_tag="OQM47_000293"
     CDS             complement(42293..42550)
                     /gene="rpmA"
                     /locus_tag="OQM47_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1478581.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42571..42882)
                     /gene="rplU"
                     /locus_tag="OQM47_000294"
     CDS             complement(42571..42882)
                     /gene="rplU"
                     /locus_tag="OQM47_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1478582.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43140..44111
                     /gene="ispB"
                     /locus_tag="OQM47_000295"
     CDS             43140..44111
                     /gene="ispB"
                     /locus_tag="OQM47_000295"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1478583.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44338..44616
                     /gene="sfsB"
                     /locus_tag="OQM47_000296"
     CDS             44338..44616
                     /gene="sfsB"
                     /locus_tag="OQM47_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1478584.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44664..45923)
                     /gene="murA"
                     /locus_tag="OQM47_000297"
     CDS             complement(44664..45923)
                     /gene="murA"
                     /locus_tag="OQM47_000297"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1478585.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45978..46232)
                     /gene="ibaG"
                     /locus_tag="OQM47_000298"
     CDS             complement(45978..46232)
                     /gene="ibaG"
                     /locus_tag="OQM47_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1478586.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46392..46685)
                     /gene="mlaB"
                     /locus_tag="OQM47_000299"
     CDS             complement(46392..46685)
                     /gene="mlaB"
                     /locus_tag="OQM47_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1478587.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46685..47320)
                     /gene="mlaC"
                     /locus_tag="OQM47_000300"
     CDS             complement(46685..47320)
                     /gene="mlaC"
                     /locus_tag="OQM47_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1478588.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47339..47890)
                     /gene="mlaD"
                     /locus_tag="OQM47_000301"
     CDS             complement(47339..47890)
                     /gene="mlaD"
                     /locus_tag="OQM47_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1478589.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47895..48677)
                     /gene="mlaE"
                     /locus_tag="OQM47_000302"
     CDS             complement(47895..48677)
                     /gene="mlaE"
                     /locus_tag="OQM47_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1478590.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48685..49494)
                     /gene="mlaF"
                     /locus_tag="OQM47_000303"
     CDS             complement(48685..49494)
                     /gene="mlaF"
                     /locus_tag="OQM47_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1478591.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49704..50669
                     /locus_tag="OQM47_000304"
     CDS             49704..50669
                     /locus_tag="OQM47_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1478592.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50683..51669
                     /gene="kdsD"
                     /locus_tag="OQM47_000305"
     CDS             50683..51669
                     /gene="kdsD"
                     /locus_tag="OQM47_000305"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1478593.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51690..52256
                     /gene="kdsC"
                     /locus_tag="OQM47_000306"
     CDS             51690..52256
                     /gene="kdsC"
                     /locus_tag="OQM47_000306"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1478594.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52253..52828
                     /gene="lptC"
                     /locus_tag="OQM47_000307"
     CDS             52253..52828
                     /gene="lptC"
                     /locus_tag="OQM47_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1478595.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52797..53354
                     /gene="lptA"
                     /locus_tag="OQM47_000308"
     CDS             52797..53354
                     /gene="lptA"
                     /locus_tag="OQM47_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1478596.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53361..54086
                     /gene="lptB"
                     /locus_tag="OQM47_000309"
     CDS             53361..54086
                     /gene="lptB"
                     /locus_tag="OQM47_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1478597.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54134..55567
                     /gene="rpoN"
                     /locus_tag="OQM47_000310"
     CDS             54134..55567
                     /gene="rpoN"
                     /locus_tag="OQM47_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1478598.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55590..55877
                     /gene="hpf"
                     /locus_tag="OQM47_000311"
     CDS             55590..55877
                     /gene="hpf"
                     /locus_tag="OQM47_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1478599.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55995..56486
                     /gene="ptsN"
                     /locus_tag="OQM47_000312"
     CDS             55995..56486
                     /gene="ptsN"
                     /locus_tag="OQM47_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1478600.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56532..57386
                     /gene="rapZ"
                     /locus_tag="OQM47_000313"
     CDS             56532..57386
                     /gene="rapZ"
                     /locus_tag="OQM47_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1478601.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57383..57655
                     /gene="npr"
                     /locus_tag="OQM47_000314"
     CDS             57383..57655
                     /gene="npr"
                     /locus_tag="OQM47_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1478602.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57868..58500
                     /gene="yrbL"
                     /locus_tag="OQM47_000315"
     CDS             57868..58500
                     /gene="yrbL"
                     /locus_tag="OQM47_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1478603.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58497..59224)
                     /gene="mtgA"
                     /locus_tag="OQM47_000316"
                     /pseudo
     CDS             complement(58497..59224)
                     /gene="mtgA"
                     /locus_tag="OQM47_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59221..59874)
                     /gene="elbB"
                     /locus_tag="OQM47_000317"
     CDS             complement(59221..59874)
                     /gene="elbB"
                     /locus_tag="OQM47_000317"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1478604.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60104..62440)
                     /gene="arcB"
                     /locus_tag="OQM47_000318"
     CDS             complement(60104..62440)
                     /gene="arcB"
                     /locus_tag="OQM47_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1478605.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62536..63465)
                     /locus_tag="OQM47_000319"
     CDS             complement(62536..63465)
                     /locus_tag="OQM47_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1478606.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64140..68600
                     /gene="gltB"
                     /locus_tag="OQM47_000320"
     CDS             64140..68600
                     /gene="gltB"
                     /locus_tag="OQM47_000320"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1478607.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68613..70031
                     /gene="gltD"
                     /locus_tag="OQM47_000321"
     CDS             68613..70031
                     /gene="gltD"
                     /locus_tag="OQM47_000321"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1478608.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70215..>70481
                     /locus_tag="OQM47_000322"
     CDS             70215..>70481
                     /locus_tag="OQM47_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478609.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQ"
BASE COUNT        17256 a        19124 c        17512 g        16589 t
ORIGIN      
        1 tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg
       61 tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac aaagtctgga
      121 tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt atggaacctt
      181 cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg ttaccggcgg
      241 cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct
      301 tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga ttgctactga
      361 tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc gtctctcggt
      421 agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt ttgtggtgcc
      481 caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc gcgtacttgt
      541 tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca gcaacgatga
      601 tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg ggatatcgag
      661 taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat gtcagtttgc
      721 ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag cgcagtgcca
      781 ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg atatccacat
      841 cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt gcgacaaatt
      901 acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca ggacgggagt
      961 ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc tctgcgggta
     1021 aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga catcagacag
     1081 gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca aacgacattc
     1141 caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag ataaaaagca
     1201 gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg tctcgttatg
     1261 gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag ccatttctcg
     1321 gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg gtcgatggaa
     1381 tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt cagggaaatc
     1441 tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt aattttggat
     1501 tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt actccagtag
     1561 actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc
     1621 gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg cgaagaagtt
     1681 cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga gttgcaaata
     1741 ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc gtcaatatgc
     1801 gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt cagggcacag
     1861 ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt
     1921 cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt cttcacgcca
     1981 tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg tagcagcgcc
     2041 agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc ttcgacaatc
     2101 agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag cagctcgcca
     2161 acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag aaccacgtag
     2221 cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg gtgggtagat
     2281 tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc acggcggcct
     2341 tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa acccgcggcg
     2401 cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat ccccgcttca
     2461 cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc ggcatcggaa
     2521 accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc tttttgttgt
     2581 tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg cagcaataaa
     2641 ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac ctctaacgta
     2701 cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag ctggccctga
     2761 gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat ttaatattga
     2821 gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt ttctcgtttg
     2881 aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg ttgtggcacc
     2941 catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga ttctgccttt
     3001 tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca attactcgcc
     3061 attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt taaccaacta
     3121 ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc agagattaaa
     3181 ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac acctgccgat
     3241 ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag ccaggggcgt
     3301 ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg cgcgaaagaa
     3361 aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca ggaacaggcg
     3421 aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga tctgcagcgc
     3481 gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc cgactggcag
     3541 aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt taacgtaaaa
     3601 gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa tggccaggca
     3661 gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat cggcactcag
     3721 ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca acctcagccg
     3781 caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct gaccggtgat
     3841 cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc agcggtaagc
     3901 gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca accacttagc
     3961 cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc gttgctgaaa
     4021 aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc actgaaccag
     4081 ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc ggaagacgaa
     4141 gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct ggtgctgatc
     4201 ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg gcagaaactg
     4261 ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg cgcgggtgtt
     4321 aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc gacaaccgac
     4381 tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga ccagttcacc
     4441 aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga aatcgctttt
     4501 atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct gtacgccagt
     4561 tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga aggcttgcag
     4621 tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca ggcgctcagc
     4681 gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga tgcctggtcg
     4741 ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa cggtaatacc
     4801 ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg gctacagtac
     4861 caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat gcgtgggaag
     4921 cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct aacgtgaacg
     4981 agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt tttgtcgagg
     5041 tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc cgcagcaaac
     5101 aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg agttttgata
     5161 ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag tggattaagg
     5221 atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga aagaattaaa
     5281 gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct tccggatgcc
     5341 atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa aatcctctgt
     5401 tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat gatcaaccgt
     5461 ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa tgttgtctta
     5521 acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt gcgggcgctg
     5581 ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg cgatattgtt
     5641 aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac cggctatgac
     5701 ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat tccttcgcat
     5761 cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg cgatctgatc
     5821 gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc attatcgcca
     5881 atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc cgtagtgggt
     5941 accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac ccaggtggac
     6001 gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca gattaagaaa
     6061 gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg gcagtcacca
     6121 aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg tgccaacgaa
     6181 gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc taacgatacg
     6241 tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa atcgtccaac
     6301 gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac tgaacgtgaa
     6361 gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt taccacagcg
     6421 tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc cgcgtcttat
     6481 caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca tccgacatgt
     6541 tttccctcaa atcagaacaa cagcgccagc ccgctgacaa tcacaccaga cactgccacc
     6601 atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat aatcagtgac
     6661 ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt tcccatacct
     6721 gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac aaacaagcat
     6781 cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc atcggcatgg
     6841 gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta aaccgggatc
     6901 gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc gctctgtgct
     6961 tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac ccatttttgc
     7021 accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat cgccgcaaaa
     7081 ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac cggattgccc
     7141 atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc cgccgcgacc
     7201 ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc cagcagcgtg
     7261 ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat cagcacctga
     7321 atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag gaaatagatc
     7381 atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag gatagattta
     7441 ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg ccatttcaca
     7501 taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca ctgaatgggc
     7561 gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa gttatgataa
     7621 ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc agctcttttg
     7681 cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat cgcctctgcc
     7741 agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg taacaaacgg
     7801 aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc cagtaacatc
     7861 gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc ttccatctcc
     7921 cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc actgaccacc
     7981 agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac cagcgtgtaa
     8041 tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc gagacaacaa
     8101 aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg cggatcatgg
     8161 ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc aatggcgtta
     8221 actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc cgttgcgcct
     8281 gtaatcagta cctgactcat cttcgttcct ttacagaatt gtctgccttg cgctccccgg
     8341 ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga aaattcgcct
     8401 catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt aatcactgat
     8461 gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc cggacacgaa
     8521 gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg agaagccagc
     8581 gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc cctgttgcta
     8641 ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac ctttacccgt
     8701 gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca gctgctgatc
     8761 agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat tattgatgtt
     8821 aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga tcagctggtt
     8881 accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg cctgctcggt
     8941 gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt cggtgaattt
     9001 gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa ccggaaagcg
     9061 acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc ggatctcacc
     9121 tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta ccgttccggc
     9181 aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg tcaaaatgta
     9241 gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga gttccccttc
     9301 ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga tggcgatgag
     9361 tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg atgcagacga
     9421 tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat accgggatca
     9481 ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg aaagcactgc
     9541 gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt tcgctggcac
     9601 tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt ctggtagcgg
     9661 aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt aaaagcgatt
     9721 gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat ccgctaaacg
     9781 atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt gatgcgccgc
     9841 cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca ctgcggcata
     9901 gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca gcccctgtcc
     9961 acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt cttcgccctg
    10021 cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat ctgtcgccac
    10081 cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg cttccgcatc
    10141 actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg gaatttctac
    10201 tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt tttcattccc
    10261 gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg catttgctcc
    10321 agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc gccttcaatc
    10381 accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc aatcatcagc
    10441 agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct gaccaccagt
    10501 ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg aatacttaac
    10561 cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa ttgccagctt
    10621 aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac cggtacactc
    10681 aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac gagttttact
    10741 ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc ggtataaatc
    10801 aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct taaatcaaaa
    10861 attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt tttgatagcg
    10921 caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc ggcgcttaag
    10981 gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga taccaatgcc
    11041 cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt gagttttgtc
    11101 catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc ggacggttac
    11161 gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc gctgatcctc
    11221 gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga acgtcatgtg
    11281 tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg ccattttgac
    11341 gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca gctggcacgg
    11401 gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgtattat gtcggaaggc
    11461 cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg tgcgtgttct
    11521 ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg cctgaacgaa
    11581 gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct gtgtaaaggg
    11641 cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag tctcaatacc
    11701 ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat tgaaggccgc
    11761 cagcgtagcc cggcgtatgt cagccaggcg gcgaaagtct ggcgtcaggc tatcgaccgt
    11821 tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac gctcgggtcg
    11881 atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca gtgagaagag
    11941 caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg ctggaagatt
    12001 tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa gcggtatgca
    12061 gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg ctcgccggaa
    12121 gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct gaactgggcg
    12181 aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat ctcggcgtgg
    12241 tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg aactgctaca
    12301 acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt atgccggtgg
    12361 agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg ggcattcgca
    12421 accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac tccgcccgct
    12481 gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc tgcattaagt
    12541 atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta ctcaatggca
    12601 ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc atgcagggat
    12661 tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg ctcgacgcct
    12721 tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc gactgtaacg
    12781 gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc tcactttgtt
    12841 aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa agtgagctgt
    12901 tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac ccgaaggctc
    12961 ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg aaaagcgcgg
    13021 ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca gtgctgcaac
    13081 atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg ggtctggcgg
    13141 cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca cactcaatac
    13201 gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg accaacggac
    13261 aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc gcgatgtcgc
    13321 ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc cggtaccagg
    13381 ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg cacaactggc
    13441 ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata tgttgttcca
    13501 ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa aaccatacgc
    13561 gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat ttctgtttac
    13621 ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac tgccgccgcc
    13681 gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc agcaggcgct
    13741 gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt cgatcctgcg
    13801 cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc aggcgcgcct
    13861 gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt gtgtcttaac
    13921 gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa gcgaagcgca
    13981 tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac tgatacaccg
    14041 gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca aacaccagaa
    14101 tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca aagcgtttac
    14161 gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc gctaaaccag
    14221 cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca acaggcgggg
    14281 caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa ccaaacagat
    14341 ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct accgcaaagt
    14401 ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg cttaatgctt
    14461 gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc ggacgaggga
    14521 tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc agcgtaccgt
    14581 acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc aggcttggca
    14641 cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc aaatacggcg
    14701 cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca tgccccagca
    14761 ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc gccgtcatgc
    14821 ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca aaaccaaaac
    14881 ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc agaatcgaac
    14941 cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg gaaatgccgt
    15001 tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa ctcgaaccga
    15061 ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata cagaaccagg
    15121 taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc actggcagag
    15181 aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca agcagcgacg
    15241 gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga aaaatagtgc
    15301 gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca gaaaaaaagc
    15361 cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg ctgttcagcg
    15421 atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga cgcggtgcgc
    15481 ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct tcacggcgtt
    15541 caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga ccaccgccac
    15601 gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc atcggcttgt
    15661 tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct ttcggcagtt
    15721 cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg ctgatgtcgc
    15781 cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg cggcccactt
    15841 caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga cgatcttcac
    15901 ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca cggtcacgga
    15961 attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt tcaccctgtg
    16021 ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct tctgcagtcg
    16081 gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc agctgctgct
    16141 gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg ttcggcagtt
    16201 ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga cgacgctcgc
    16261 ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta cgaccgatac
    16321 ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg ctgatacgct
    16381 caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga cgaccgtctt
    16441 taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc agcgcggcgc
    16501 tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt ttggtacgaa
    16561 cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt gcttcgtttt
    16621 tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta gtcacgctgg
    16681 actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga cgaatcgctt
    16741 ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc gccataatgg
    16801 tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc agaaccagac
    16861 cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga cggcccggag
    16921 taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca taacgctggc
    16981 cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc gtcattgctt
    17041 cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt ggtgctttca
    17101 gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt ccgctccccg
    17161 tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac tctgcctgaa
    17221 ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga gccttcaggc
    17281 ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc aaaattaatg
    17341 gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga aaagtgtgaa
    17401 ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag gtgatggcaa
    17461 tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt ctgccaggtc
    17521 atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct caacaaagtt
    17581 atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca aatcccccag
    17641 acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag cgagcgaggt
    17701 gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa tgttgcccag
    17761 gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga agtgctgttt
    17821 caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa gccacagact
    17881 acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt gcgctaactt
    17941 gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt tgtaagttgg
    18001 atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc ctgcctgcgt
    18061 taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg ccagcgcttg
    18121 cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca ctccgcgctc
    18181 atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga tttgttccat
    18241 acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga ggacttcact
    18301 tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa cgaaacacca
    18361 gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac gtcctgttcc
    18421 cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg agggcaaatg
    18481 gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag gttgagactg
    18541 ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt ccagaacttt
    18601 caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc gtttgtcagc
    18661 gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa atgcgccaaa
    18721 gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt ctgcagtgat
    18781 ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg caatcttcac
    18841 agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg cacggataac
    18901 agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga tggtatggat
    18961 acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt ccattacgcc
    19021 caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga tctctttggt
    19081 gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg aacccgcaac
    19141 tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca gtacaacgta
    19201 gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt tgatcggcac
    19261 acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag aggaaccgtt
    19321 ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca tatccggcat
    19381 gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac gcttcggaga
    19441 accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga acaggaaggt
    19501 atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag tacccagcgt
    19561 tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt gagtacgcgg
    19621 cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac cgtcgatacg
    19681 cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt gcagaatttc
    19741 acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga tggtttcaga
    19801 tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt aagcatcgct
    19861 caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta ccggctccgg
    19921 ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt tctgaataac
    19981 aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt cgctcagcag
    20041 ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa ccagatccag
    20101 tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat tgatgtaacc
    20161 tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca gtgctgcgga
    20221 agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa ccacggtggc
    20281 gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg ggcggtcaat
    20341 cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga agaagctacc
    20401 cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg ggaagaagtc
    20461 ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg tgtcatccat
    20521 gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca gagtcacggt
    20581 gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat cctttctctt
    20641 tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca tcctcgcgac
    20701 taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg cgcgaacctc
    20761 tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg ctgccgtcag
    20821 cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt agcgacgcag
    20881 acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca ggtagtcgag
    20941 cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt gatctttttt
    21001 gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg ctacctgaac
    21061 ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt tagctgttgc
    21121 ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga cgccgatctc
    21181 taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc gacgttaagc
    21241 ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac gccgggtatt
    21301 caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc ataccgataa
    21361 atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca ccggatgtac
    21421 gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc accaccgggt
    21481 agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc gcagccggaa
    21541 tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc acaatccgtt
    21601 caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg ccacaaccga
    21661 gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag cagtgaattt
    21721 ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg gtaatcgggc
    21781 gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt ttaccctgat
    21841 atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa gtatccagcg
    21901 ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt tgtccgtcgg
    21961 catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt ttgtcggagt
    22021 ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac atgccagttg
    22081 ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc cgtttcactt
    22141 tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa acgccgttaa
    22201 tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt ccgggttaac
    22261 acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc gcatcccttc
    22321 aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc gcatcgcttt
    22381 ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc ccgctttaac
    22441 cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg ccaggtcgcg
    22501 agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt taatttcacg
    22561 ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg ggcgaccaaa
    22621 ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct ttttaaaatt
    22681 aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg tcacatcagg
    22741 caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa aaaccttaag
    22801 cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca gtgcggacgt
    22861 cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg ttaacgtcat
    22921 ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg tcacgcagaa
    22981 cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct gcgatggcac
    23041 caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc tgctgtttca
    23101 gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg ttatagatga
    23161 cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca gaggcatcag
    23221 cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg gcgtcggttt
    23281 cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca gtagacagtt
    23341 tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc aggacgatgt
    23401 tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt ttagatttct
    23461 gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca acttcacgcg
    23521 ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg ccggacaggc
    23581 cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc agttcgttac
    23641 gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc ttgtgcagag
    23701 taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg aaggattcga
    23761 taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact tccgcctgca
    23821 gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac acatgtacga
    23881 actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt tcgttcttaa
    23941 cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc accggaacct
    24001 gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg tcgtcggcag
    24061 caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt gaagtaaacg
    24121 cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca acgtggtatg
    24181 caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt gaacgaatgt
    24241 agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca accggcgcgc
    24301 gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc agctcgtttt
    24361 cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg gtttcctgat
    24421 cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg atgacctgag
    24481 agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg ccgatcacaa
    24541 cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa gaacctttac
    24601 gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca gcttttgatt
    24661 cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca cggccacggc
    24721 cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga gaagtagtga
    24781 cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg ttttcttccg
    24841 ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta cgacgcgctt
    24901 cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct ttttcagcct
    24961 gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc gcagcttcac
    25021 gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt tcagcttttt
    25081 gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct tccgctgcaa
    25141 ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg acttcgattt
    25201 gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt gttttacgtt
    25261 gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc aaagtctgtt
    25321 tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca gcaaattgct
    25381 gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc gttacatctg
    25441 tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag caaatattac
    25501 gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata tcagccagat
    25561 catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg gccagtttga
    25621 atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt ttgttatcac
    25681 cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca cgctcgcgca
    25741 gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc atcggcacat
    25801 aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg aagtcttcgt
    25861 cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc tgatgcttag
    25921 cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc gaagccagac
    25981 gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca acggcgatat
    26041 ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt gccattgcgt
    26101 taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc tcgccgccca
    26161 gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca cctaccggat
    26221 cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg tcgcgagccg
    26281 ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg aacagttcga
    26341 tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct tccgggcgaa
    26401 cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc ggcagcatat
    26461 cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg ttgtcgcggt
    26521 ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac tgatcaacca
    26581 ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc gtctgggtgg
    26641 tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc aagttcaggc
    26701 tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg gtgacttcat
    26761 caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg atctgtacac
    26821 ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg ctttccaatg
    26881 cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca actacagcca
    26941 aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag tggggaacca
    27001 ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg acggtaactg
    27061 tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg ttttgtaccg
    27121 ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt tcagccgtga
    27181 acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg gtgatgggat
    27241 cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca tcaacattga
    27301 tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga ataaattcga
    27361 tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct gttaattttt
    27421 gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag cccagttatt
    27481 ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg aaccctataa
    27541 ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac tacgaaggcc
    27601 gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg ggggccggat
    27661 ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgc
    27721 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
    27781 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
    27841 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
    27901 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
    27961 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
    28021 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
    28081 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
    28141 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
    28201 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
    28261 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
    28321 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
    28381 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
    28441 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
    28501 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
    28561 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
    28621 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
    28681 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
    28741 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
    28801 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
    28861 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
    28921 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
    28981 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
    29041 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
    29101 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
    29161 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
    29221 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
    29281 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
    29341 tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata tggaaaacaa
    29401 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
    29461 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
    29521 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
    29581 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
    29641 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
    29701 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
    29761 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
    29821 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
    29881 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
    29941 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
    30001 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
    30061 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
    30121 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
    30181 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
    30241 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
    30301 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
    30361 atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata cggcaccgtg
    30421 tttgcaattg agagtaatct ttgatgcgct ttcgctgcta aagcaaaata gttgagattg
    30481 aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa cgctttttta
    30541 ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga aatgacaatc
    30601 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattact
    30661 gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata catccccagc
    30721 attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
    30781 gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac
    30841 acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt aacaaccagg
    30901 atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga ggcgactaag
    30961 ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt aaaatcttct
    31021 agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta
    31081 tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata tcagaccgaa
    31141 acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta attaaataac
    31201 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
    31261 agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca
    31321 caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc gtaccggcac
    31381 catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa
    31441 ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc ttttctggaa
    31501 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
    31561 agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc
    31621 tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt gcactcagat
    31681 tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc accagactga
    31741 tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta ccagaaccac
    31801 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
    31861 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta
    31921 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
    31981 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaaac
    32041 gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa
    32101 accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac
    32161 gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
    32221 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcagcaacac
    32281 gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa ctgactcatg
    32341 ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct gcgggaacat
    32401 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg ccgccagcac
    32461 ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc
    32521 ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
    32581 acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat
    32641 gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa gaccttcacg
    32701 cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat
    32761 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
    32821 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg
    32881 ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg
    32941 ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt
    33001 cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga tacggctggc
    33061 tttacccagt tcagccgaat caacgcagct gatctccttt tccatttccg cttcgatggc
    33121 ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa tcccattatc
    33181 gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt
    33241 cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc
    33301 cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
    33361 aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
    33421 aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
    33481 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
    33541 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca
    33601 ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca
    33661 gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt
    33721 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
    33781 atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
    33841 gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga gcttcctgca
    33901 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg
    33961 cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga gcgccaactt
    34021 tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc
    34081 gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt
    34141 ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcattga
    34201 tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa
    34261 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
    34321 taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag aaaaaacccc
    34381 ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc
    34441 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
    34501 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
    34561 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt
    34621 ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag
    34681 ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg
    34741 tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga atacttcacc
    34801 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
    34861 gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc
    34921 cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc
    34981 cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
    35041 cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg gcaccaggcg
    35101 accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg cttccgtcat
    35161 caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac
    35221 catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt
    35281 cgccaggtca gcaccggaga aaccaggagt accacgagca atgattgccg cgtcgatatc
    35341 gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc
    35401 tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg ccgggtcgag
    35461 aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc
    35521 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc
    35581 agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc
    35641 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
    35701 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt
    35761 cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt
    35821 accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt cagcaacttc
    35881 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt
    35941 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
    36001 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa
    36061 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
    36121 caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
    36181 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
    36241 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
    36301 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
    36361 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
    36421 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
    36481 ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct tacgaacttt
    36541 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
    36601 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag
    36661 ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca tgtttggtgc
    36721 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
    36781 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
    36841 taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga
    36901 ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
    36961 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct ttttctgtgc
    37021 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
    37081 cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat
    37141 ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc
    37201 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
    37261 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
    37321 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
    37381 aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
    37441 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
    37501 cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt
    37561 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcagagcggc
    37621 ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt
    37681 ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat
    37741 cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt
    37801 taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatccagat
    37861 tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat
    37921 caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc
    37981 cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg
    38041 cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt
    38101 cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt
    38161 gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac
    38221 tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac
    38281 aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg tttactttat
    38341 agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat
    38401 tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg
    38461 ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg gtgcaaaaag
    38521 tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta
    38581 cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca
    38641 ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg
    38701 cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt
    38761 tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg
    38821 ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc
    38881 cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa
    38941 agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc
    39001 aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtggtgc
    39061 caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc
    39121 cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt
    39181 taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac
    39241 ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc
    39301 tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc catgcgcgct
    39361 tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc ctgcgccagc
    39421 aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt tcgcggcata
    39481 acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg
    39541 aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg cagtaccgtg
    39601 caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt tcgttgcagg
    39661 gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcatttgtac
    39721 aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt attccgttag
    39781 tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca aaaagaaacc
    39841 ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat gccggatgcg
    39901 gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc aatcatcttg
    39961 taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt gattaacgct
    40021 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat
    40081 catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac atgaattcga
    40141 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    40201 tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc agataatatt
    40261 tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct tctgccttat
    40321 ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga tcctggctgt
    40381 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
    40441 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgaa
    40501 tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
    40561 agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat
    40621 aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat ttacccgcgt
    40681 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    40741 tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac
    40801 gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttag
    40861 tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    40921 taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc
    40981 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg
    41041 cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg cctttcggaa
    41101 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    41161 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    41221 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    41281 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    41341 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    41401 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    41461 aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg agctgccagt
    41521 ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt gtacataaag
    41581 tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    41641 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc caggtgtaat
    41701 cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc agaccgctca
    41761 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    41821 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    41881 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    41941 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca
    42001 ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    42061 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    42121 ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    42181 aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa
    42241 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    42301 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    42361 ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    42421 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    42481 taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    42541 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    42601 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    42661 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    42721 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    42781 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    42841 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    42901 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    42961 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aacacaactt
    43021 gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt cctaaccata
    43081 aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg gcttttgcga
    43141 tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa
    43201 tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat tacatcgtca
    43261 gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga gctgttggct
    43321 atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac acggcgactc
    43381 tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct accgccaacg
    43441 ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc cgcgcgttcc
    43501 agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa gctgtaaacg
    43561 tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac atcactgaag
    43621 aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt
    43681 gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag gattatgggc
    43741 gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat gccgatggcg
    43801 aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc
    43861 tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc gccatcgaac
    43921 aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct tgtggatctc
    43981 ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca gcgttacagg
    44041 tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc gctgttcaac
    44101 gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc
    44161 ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgatgaatc tttatatatt
    44221 ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag gagtatagtg
    44281 atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg aggaaagatg
    44341 gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg caagaaagga
    44401 acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca
    44461 ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg aactgacccc
    44521 tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat cgacagaacg
    44581 cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa
    44641 cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt tgcacctaaa
    44701 gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat acgatcaacc
    44761 accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc acgcagatcg
    44821 gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac ggtattgctt
    44881 tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa gcggttttca
    44941 aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag cgtgaactgg
    45001 gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac agccttcgga
    45061 cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc cgctccagca
    45121 tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg gcagataatt
    45181 ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg atccggcagc
    45241 acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg atcggtgccc
    45301 tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac gatttccggt
    45361 tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc acacatgatg
    45421 gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt cagacgacca
    45481 tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg
    45541 ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg tagtgaaact
    45601 tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc acgcatggtt
    45661 ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat atgcacagaa
    45721 ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt atcgacgtct
    45781 ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc ggcaaaaaga
    45841 ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag cttcgttggc
    45901 ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat atccgctacc
    45961 ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg
    46021 gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc catcagcggg
    46081 ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc acccacggca
    46141 ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga gagagcgttc
    46201 atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag ttaataaacc
    46261 agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc tcgctgataa
    46321 atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg ccatatccgg
    46381 cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt cgcgagggta
    46441 tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt cgccagatcg
    46501 ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg gctaagatcg
    46561 atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag cagaacgtcc
    46621 tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct cagtgactcg
    46681 ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc agttgcgcag
    46741 tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt ttggtggtga
    46801 tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc tgggagtttt
    46861 tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata atggtaacgc
    46921 gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga taggtttgac
    46981 cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa
    47041 agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc accagcgcac
    47101 cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta cgcagataat
    47161 ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg
    47221 cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca ctcagaggtg
    47281 caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc tcctgaaatt
    47341 atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca gctggcgcat
    47401 cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca atgagatctt
    47461 ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc
    47521 ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc agcaggccgg
    47581 aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt tcaatttcca
    47641 gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc cggcccacaa
    47701 caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg
    47761 cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa
    47821 caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt tcattttttt
    47881 tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc cagccccaga
    47941 acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc ggcagacgtc
    48001 gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc gaacaccacg
    48061 ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg cattgccgac
    48121 cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc gccccagatc
    48181 cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg
    48241 ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga gagttgctct
    48301 gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc ggcaaacaac
    48361 agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag catacccaga
    48421 ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa caccattccg
    48481 atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt atagagctgg
    48541 cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc attgaataac
    48601 attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc
    48661 agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt gataatcgcc
    48721 ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact gacgtacgcg
    48781 cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt ttttgtccgc
    48841 caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag aaaccaccac
    48901 acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta cgcccatggt
    48961 gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct ccagcgcaat
    49021 cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag gcattagttt
    49081 agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat gcaacaatgg
    49141 cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc
    49201 agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg tatacaggcg
    49261 cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat gatctggtgc
    49321 gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg gccccatgat
    49381 cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc agcgattgcc
    49441 acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct ccataattca
    49501 cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga acccaacata
    49561 tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac tttttagccg
    49621 cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg gaccggcgag
    49681 tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt gttggtttac
    49741 ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt tgccgaacct
    49801 ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca tcgttaccag
    49861 aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc gttggtacag
    49921 ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg ctggttcgtc
    49981 cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg ttggtcagcg
    50041 tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt atctttctcc
    50101 tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag gggactgaca
    50161 gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc gtcgcgtttt
    50221 tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg gttgataacg
    50281 ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg acggcaattg
    50341 ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc aaaggtgaaa
    50401 acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc atcgtgttgg
    50461 gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt cgtgactaca
    50521 gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc tccccgcaac
    50581 cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg gcgatgttgt
    50641 actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca cgtagagtta
    50701 caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga acgtgaatgc
    50761 ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga aaagatgttc
    50821 tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat cgggcgcaaa
    50881 atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc tagtgaagcc
    50941 gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat ctctaactca
    51001 ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca cgtaccgtta
    51061 atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt gcatctgtgt
    51121 gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag caccaccgcc
    51181 acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg ctttactgct
    51241 gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct gctgcgcgta
    51301 aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc cagtctgcgt
    51361 gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg cgatgacaat
    51421 atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga tatgggcgtg
    51481 gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg tgtgcgccct
    51541 ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac ctccgtgatg
    51601 gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact gcgtgcaggc
    51661 gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg cttgcgacct
    51721 gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt ctgctgatcc
    51781 tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac ggcgaagagc
    51841 tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc tctgatattg
    51901 aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt gccacattgg
    51961 ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc gatttgctgg
    52021 aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc atcgactggc
    52081 cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca ctgttgatcc
    52141 cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc gaagtttgcg
    52201 acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg atatgagtaa
    52261 agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga tcggcattaa
    52321 tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc ccacctataa
    52381 aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct atcgattgat
    52441 tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc agccggtact
    52501 taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata aagccaagct
    52561 gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac ttgtgccaga
    52621 ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga cgcaggatgt
    52681 tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg gtctgaaaat
    52741 gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa catcctatga
    52801 aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt ctggccgcca
    52861 gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt gaatcggacc
    52921 agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc atcgtcaccc
    52981 agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc ggcgaacaag
    53041 gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg caggacaacg
    53101 gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa gattttgtcg
    53161 ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc gataagatca
    53221 cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag cgcgtaacca
    53281 ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc ccggcacaga
    53341 agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca aaagcctata
    53401 aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa attgtcggtc
    53461 tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta ggcattgtgc
    53521 cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg cctctgcatg
    53581 cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc cgtcgcctca
    53641 gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct gctgaacaac
    53701 gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg cgtgacagca
    53761 tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc gcactggctg
    53821 cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg atctcggtta
    53881 tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg ctgatcactg
    53941 accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc agtcaggggc
    54001 atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt aagcgtgtat
    54061 accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt agcaggagag
    54121 tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac tggcgatgac
    54181 gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac ttcagcagga
    54241 gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc atgaagaaat
    54301 cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc tcgaacaaaa
    54361 agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca ccgctggtac
    54421 accatccggc accagcggtg actacattga cgacgagctg ccggtctacc agggcgaaac
    54481 gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt tttccgacac
    54541 tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt atctgactgt
    54601 cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg acgaggttga
    54661 agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa aagatctgcg
    54721 tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc tggaagaggc
    54781 cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc gcactttaat
    54841 gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga tccagtcgct
    54901 cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca ttccagatgt
    54961 gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca gcattccgcg
    55021 tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg atggtgacag
    55081 ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc tggaaagccg
    55141 taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag ccttctttga
    55201 gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg ctgtcgaaat
    55261 gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc cacgaggcat
    55321 ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg gcgaagcttc
    55381 ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc cagcgaaacc
    55441 gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg tggcacgccg
    55501 cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc gtaaacagct
    55561 cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga aataacgtcg
    55621 agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt gagcaatatt
    55681 ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc cacacctcag
    55741 atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt caggacatgt
    55801 acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa cataaagata
    55861 aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct gttgtgcggc
    55921 acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga gtgggcaggt
    55981 tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt gttcttaaca
    56041 gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg gaaatcatca
    56101 gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa gctatcctga
    56161 cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg catggcaaac
    56221 ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg cctatagctt
    56281 tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg ccggcagacc
    56341 aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg gacaaaacca
    56401 tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc attacggata
    56461 ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga cgttgtgagg
    56521 agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt aaatctgtcg
    56581 ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc gtagtgttgt
    56641 tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc agcattgatg
    56701 ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac ctgcctgacg
    56761 ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt cgtcgttaca
    56821 gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa agtgctatcg
    56881 acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt gtcgatacct
    56941 cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg ggtaaacgtg
    57001 aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc cctatcgatg
    57061 cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg aaactgcgtc
    57121 caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca gaagtacaca
    57181 attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg gaaaccaaca
    57241 accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt tcggtgtata
    57301 ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag tcacgccatc
    57361 gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca caaacaagct
    57421 gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt ttgacgctga
    57481 agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta ttgcgctgct
    57541 gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc cacaggaaga
    57601 ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag attaatcttc
    57661 atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc acgtactgaa
    57721 atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca gaagaacctt
    57781 aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca tactggagat
    57841 cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag tcccctgggc
    57901 accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg tatcaagatt
    57961 gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa gtactacgcg
    58021 catcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg taccgttgag
    58081 acggattgcg gaaccggtta tgtctacgat gtgattgccg attttgacgg caagccttcc
    58141 attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc acaactgcgc
    58201 cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac gatgtcgctg
    58261 aagccgcaga atatcctctg tcaccgcatc agtgagtcag aggttatccc ggtcgtctgc
    58321 gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg gtgttgtctg
    58381 cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc tattgccctg
    58441 cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa
    58501 tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg ccgtaaaatc
    58561 cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg aagtggatta
    58621 ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg
    58681 aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc ggcgatattc
    58741 aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag cgttaatcca
    58801 gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt ttggccgtct
    58861 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    58921 ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg
    58981 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    59041 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    59101 agaagggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    59161 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    59221 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    59281 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    59341 gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    59401 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    59461 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    59521 ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc ttaaattctt
    59581 cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    59641 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat tgcgcgtttc
    59701 cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    59761 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    59821 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    59881 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    59941 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    60001 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    60061 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    60121 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    60181 tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt tgctgaccca
    60241 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    60301 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    60361 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    60421 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    60481 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    60541 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    60601 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    60661 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    60721 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    60781 catcaacgct gttacctaat ttttccagca cagaacgcgc gacaatcacg ttcagttcaa
    60841 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    60901 catcgacctc ttctgccacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    60961 cctgttcact ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    61021 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    61081 acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagaa tcttccactt
    61141 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    61201 tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc gtcccgtcgg
    61261 taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat cctttttgtt
    61321 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggct
    61381 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttaaaga
    61441 tattccccag cgtgacggcc gaaacatgga tagttttgag atatttttcc tgctcggcgg
    61501 tgagttcggt atccagcaga atgcggctca ggccgacgat accgttcagc ggtgtacgca
    61561 attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcgcgt tcaagcgcat
    61621 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    61681 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc ccgtccgggt
    61741 aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    61801 caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    61861 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    61921 cttcgttacg ataaaaaacc aagtcgggtg aagcatcaag gaaggaacgt aagaatgagg
    61981 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    62041 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc tgatttagct
    62101 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    62161 gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    62221 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    62281 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    62341 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    62401 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    62461 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    62521 attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    62581 tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc gattttcgca
    62641 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    62701 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtataatcct ccagttcaat
    62761 accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca caatatgcag
    62821 cggatgcagc tttatgccgt ccacgctggt ttcaaccact cgctccagcg tttgcaaaca
    62881 ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    62941 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    63001 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    63061 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    63121 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    63181 ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc gtttagcgcg
    63241 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    63301 atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    63361 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    63421 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    63481 gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga cctggatcaa
    63541 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcac
    63601 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    63661 aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa tgctggtttt
    63721 ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag gaattgactg
    63781 tttctctaac gactctcctt ttagccttaa agataaaatc tattttaatt tcagctattt
    63841 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    63901 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    63961 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    64021 caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg
    64081 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    64141 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    64201 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    64261 accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg ctgttacaaa
    64321 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    64381 acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc gcacgccgca
    64441 tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca
    64501 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt gagcaaattt
    64561 ttgtgaacgc cccggcaggc tggcgtccac gcgatatgga gcgtcgtctg tttatcgccc
    64621 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    64681 atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc ttttatctgg
    64741 atcttgcgga cctgcgtctg gaatcggcca tttgcctgtt ccaccagcgc ttctccacta
    64801 acaccgtacc gcgctggccg ctggcgcaac cgttccgcta tctggcgcat aacggtgaaa
    64861 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    64921 cgctgatccc tgacctgcac gacgccgcgc cattcgtcaa cgaaaccggt tctgactcca
    64981 gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc atccgcgcca
    65041 tgcgcctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    65101 ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggcg ggcatcgtga
    65161 tgtccgatgg tcgcttcgct gcctgtaacc tcgaccgtaa cggtctgcgt ccggcacgct
    65221 acgtcattac caaagacaag ctcatcacct gcgcctctga agtcggtatc tgggattacc
    65281 agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg gtgatcgaca
    65341 ctcgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa agccgccacc
    65401 cttataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa gatctgcccg
    65461 atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac cagaaacagt
    65521 ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa aacggtcagg
    65581 aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt cagccgcgca
    65641 ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca atcgacccgc
    65701 tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt
    65761 gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    65821 atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg ctggatatca
    65881 cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctatgc gacaaagccg
    65941 aaaaaatggt acgtagcggc accgtgttgc tggtgctctc cgaccggaat atcgctaaag
    66001 atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt ctggtcgatc
    66061 aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc
    66121 acttcgccgt gttgctgggt ttcggcgcga cggctattta tccgtacctt gcctatgaaa
    66181 cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc gtgatgctca
    66241 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    66301 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctacac gatgatgtag
    66361 tgggcctgtg cttccagggc gcggtcagcc gcattggtgg ggcaagcttt gaagacttcc
    66421 agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgcaagccg attagccagg
    66481 gcggcctgct gaaatacgtc cacggcggcg aataccacgc ctacaacccg gacgtggtgc
    66541 gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    66601 tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg ccgggtgaaa
    66661 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc tttgataccg
    66721 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    66781 atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg cgctatggca
    66841 ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt actccggcgt
    66901 atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    66961 gtggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    67021 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaagacttag
    67081 cgcagcttat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gttaagctgg
    67141 tgtccgaacc aggcgttggc accatcgcga ctggcgtggc gaaagcttat gcggacttga
    67201 tcaccatcgc gggctatgac ggcggcaccg gcgcaagccc gctgtcgtca gtgaaatatg
    67261 caggctgccc gtgggaactc ggtctggtgg aaacccaaca ggcgctggtt gccaacggct
    67321 tgcgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggtgtc gatatcatca
    67381 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg gtggcgctcg
    67441 gctgtaaata tctgcgtatt tgccacctga acaactgcgc cacgggtgta gcaactcagg
    67501 atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgact aattactttg
    67561 agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggagtaacg cgtctggtgg
    67621 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    67681 aactggcgct gtcgaagctg ctggagactg ccgagccaca tgcaggtaag tcactctact
    67741 gcactgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg ctgcaacagg
    67801 cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaacaccg
    67861 accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    67921 tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt ttcggtgtgt
    67981 ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat gtcggtaaag
    68041 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc cgcagccatg
    68101 aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    68161 caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc gtggtagaag
    68221 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    68281 ccggcgttaa cttcggcgcg ggcatgaccg gcggcttcgc ttacgttctc gatgaaagcg
    68341 gtgatttccg caaacgcgtt aacccggaac tggtcgaagt cttaagcgtc gacgatctgg
    68401 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat accggttctc
    68461 agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc gcgctggtta
    68521 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    68581 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    68641 acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgtaaaatt gagtttgttg
    68701 aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc tgtctgtcgt
    68761 gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca aactggctga
    68821 agctcgccaa cgaggggcgt atttttgaag cggcagaact gtcgcatcag accaacacac
    68881 tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc
    68941 tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    69001 cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt aaaaaagtgg
    69061 cgattatcgg tgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg cgtaacggcg
    69121 taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc ttcggtattc
    69181 cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc accggcatgg
    69241 gtattgaatt caaactcaat accgaagtgg gccgcgacgt acagctggac gatctgctga
    69301 gtgattacga tgccgtgttc cttggcgttg ggacttatca gtcaatgcgc ggcgggctgg
    69361 aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    69421 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    69481 tggtagtact tggcggtggc gacactgcga tggactgcgt gcgcacgtct gtacgccagg
    69541 gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    69601 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccactgg
    69661 ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt accgaaatgg
    69721 gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc gaacatatcg
    69781 ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    69841 caaaacacag cgtcgagctg gattcacaag gccgcatcat cgctccggaa ggcaacgaca
    69901 acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    69961 atctggtcgt taccgccatc gccgaaggtc gcaaagcggc agacggcatt atgaactggc
    70021 tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct
    70081 cctttctttc tggaaatcgc ctcgcataat ctgaagaaaa ccaatgtaat acgctcatct
    70141 ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg cagcgccata
    70201 taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac cagcaaatcc
    70261 acaacggcat tattcatctg gtcgatagcg cccgaacgga aacggtacgt agcgttaacg
    70321 cgctgatgac cgcgacgtac tgggaaattg gccgacggat tgtcgaattt gaacaaggtg
    70381 gtgaggccag agcagcttat ggtgcacagt tgattgatcg gctatcgaag gatttaagtc
    70441 gacggtataa acgtggggtc tctgcaaaaa acttacgcca a
//
LOCUS       DAJVPT010000005        67470 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVPT010000005 DAJVPT010000000
VERSION     DAJVPT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            420..1059
                     /locus_tag="OQM47_000323"
                     /pseudo
     CDS             420..1059
                     /locus_tag="OQM47_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1162..1485
                     /locus_tag="OQM47_000324"
     CDS             1162..1485
                     /locus_tag="OQM47_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCS1478610.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1552..3009)
                     /gene="nuoN"
                     /locus_tag="OQM47_000325"
     CDS             complement(1552..3009)
                     /gene="nuoN"
                     /locus_tag="OQM47_000325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1478611.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3016..4545)
                     /gene="nuoM"
                     /locus_tag="OQM47_000326"
     CDS             complement(3016..4545)
                     /gene="nuoM"
                     /locus_tag="OQM47_000326"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1478612.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4709..6550)
                     /gene="nuoL"
                     /locus_tag="OQM47_000327"
     CDS             complement(4709..6550)
                     /gene="nuoL"
                     /locus_tag="OQM47_000327"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1478613.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6547..6849)
                     /gene="nuoK"
                     /locus_tag="OQM47_000328"
     CDS             complement(6547..6849)
                     /gene="nuoK"
                     /locus_tag="OQM47_000328"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1478614.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6846..7400)
                     /gene="nuoJ"
                     /locus_tag="OQM47_000329"
     CDS             complement(6846..7400)
                     /gene="nuoJ"
                     /locus_tag="OQM47_000329"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1478615.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7412..7954)
                     /gene="nuoI"
                     /locus_tag="OQM47_000330"
     CDS             complement(7412..7954)
                     /gene="nuoI"
                     /locus_tag="OQM47_000330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1478616.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7969..8946)
                     /gene="nuoH"
                     /locus_tag="OQM47_000331"
     CDS             complement(7969..8946)
                     /gene="nuoH"
                     /locus_tag="OQM47_000331"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1478617.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8943..11669)
                     /gene="nuoG"
                     /locus_tag="OQM47_000332"
     CDS             complement(8943..11669)
                     /gene="nuoG"
                     /locus_tag="OQM47_000332"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1478618.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11722..13059)
                     /gene="nuoF"
                     /locus_tag="OQM47_000333"
     CDS             complement(11722..13059)
                     /gene="nuoF"
                     /locus_tag="OQM47_000333"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1478619.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13056..13556)
                     /gene="nuoE"
                     /locus_tag="OQM47_000334"
     CDS             complement(13056..13556)
                     /gene="nuoE"
                     /locus_tag="OQM47_000334"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1478620.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13559..15361)
                     /gene="nuoC"
                     /locus_tag="OQM47_000335"
     CDS             complement(13559..15361)
                     /gene="nuoC"
                     /locus_tag="OQM47_000335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1478621.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15455..16117)
                     /gene="nuoB"
                     /locus_tag="OQM47_000336"
     CDS             complement(15455..16117)
                     /gene="nuoB"
                     /locus_tag="OQM47_000336"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1478622.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16133..16576)
                     /gene="nuoA"
                     /locus_tag="OQM47_000337"
     CDS             complement(16133..16576)
                     /gene="nuoA"
                     /locus_tag="OQM47_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1478623.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17207..18145)
                     /gene="lrhA"
                     /locus_tag="OQM47_000338"
     CDS             complement(17207..18145)
                     /gene="lrhA"
                     /locus_tag="OQM47_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1478624.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19064..20281
                     /gene="alaA"
                     /locus_tag="OQM47_000339"
     CDS             19064..20281
                     /gene="alaA"
                     /locus_tag="OQM47_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1478625.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20365..20964
                     /gene="yfbR"
                     /locus_tag="OQM47_000340"
     CDS             20365..20964
                     /gene="yfbR"
                     /locus_tag="OQM47_000340"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1478626.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21023..22855)
                     /locus_tag="OQM47_000341"
     CDS             complement(21023..22855)
                     /locus_tag="OQM47_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1478627.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22942..23592)
                     /gene="hxpA"
                     /locus_tag="OQM47_000342"
     CDS             complement(22942..23592)
                     /gene="hxpA"
                     /locus_tag="OQM47_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1478628.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23603..24097)
                     /locus_tag="OQM47_000343"
     CDS             complement(23603..24097)
                     /locus_tag="OQM47_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1478629.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24180..24635)
                     /locus_tag="OQM47_000344"
     CDS             complement(24180..24635)
                     /locus_tag="OQM47_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1478630.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24973..26175
                     /gene="ackA"
                     /locus_tag="OQM47_000345"
     CDS             24973..26175
                     /gene="ackA"
                     /locus_tag="OQM47_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1478631.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26250..28394
                     /gene="pta"
                     /locus_tag="OQM47_000346"
     CDS             26250..28394
                     /gene="pta"
                     /locus_tag="OQM47_000346"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1478632.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28584..30104
                     /gene="yfcC"
                     /locus_tag="OQM47_000347"
     CDS             28584..30104
                     /gene="yfcC"
                     /locus_tag="OQM47_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1478633.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30137..30679)
                     /gene="yfcD"
                     /locus_tag="OQM47_000348"
     CDS             complement(30137..30679)
                     /gene="yfcD"
                     /locus_tag="OQM47_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1478634.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30737..31288)
                     /gene="yfcE"
                     /locus_tag="OQM47_000349"
     CDS             complement(30737..31288)
                     /gene="yfcE"
                     /locus_tag="OQM47_000349"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1478635.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31344..31988)
                     /gene="yfcF"
                     /locus_tag="OQM47_000350"
     CDS             complement(31344..31988)
                     /gene="yfcF"
                     /locus_tag="OQM47_000350"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1478636.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32124..32771
                     /gene="yfcG"
                     /locus_tag="OQM47_000351"
     CDS             32124..32771
                     /gene="yfcG"
                     /locus_tag="OQM47_000351"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1478637.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32828..33190
                     /gene="folX"
                     /locus_tag="OQM47_000352"
     CDS             32828..33190
                     /gene="folX"
                     /locus_tag="OQM47_000352"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1478638.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33211..34104
                     /locus_tag="OQM47_000353"
     CDS             33211..34104
                     /locus_tag="OQM47_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1478639.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQIRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34152..35042)
                     /gene="rpnB"
                     /locus_tag="OQM47_000354"
     CDS             complement(34152..35042)
                     /gene="rpnB"
                     /locus_tag="OQM47_000354"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1478640.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35239..36012)
                     /gene="hisP"
                     /locus_tag="OQM47_000355"
     CDS             complement(35239..36012)
                     /gene="hisP"
                     /locus_tag="OQM47_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1478641.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36020..36736)
                     /locus_tag="OQM47_000356"
     CDS             complement(36020..36736)
                     /locus_tag="OQM47_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1478642.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36733..37419)
                     /gene="hisQ"
                     /locus_tag="OQM47_000357"
     CDS             complement(36733..37419)
                     /gene="hisQ"
                     /locus_tag="OQM47_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1478643.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37509..38291)
                     /gene="hisJ"
                     /locus_tag="OQM47_000358"
     CDS             complement(37509..38291)
                     /gene="hisJ"
                     /locus_tag="OQM47_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1478644.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38512..39294)
                     /gene="argT"
                     /locus_tag="OQM47_000359"
     CDS             complement(38512..39294)
                     /gene="argT"
                     /locus_tag="OQM47_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1478645.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39560..40129)
                     /gene="ubiX"
                     /locus_tag="OQM47_000360"
     CDS             complement(39560..40129)
                     /gene="ubiX"
                     /locus_tag="OQM47_000360"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1478646.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40224..41741)
                     /gene="purF"
                     /locus_tag="OQM47_000361"
     CDS             complement(40224..41741)
                     /gene="purF"
                     /locus_tag="OQM47_000361"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1478647.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41778..42266)
                     /gene="cvpA"
                     /locus_tag="OQM47_000362"
     CDS             complement(41778..42266)
                     /gene="cvpA"
                     /locus_tag="OQM47_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1478648.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42525..43187)
                     /gene="dedD"
                     /locus_tag="OQM47_000363"
     CDS             complement(42525..43187)
                     /gene="dedD"
                     /locus_tag="OQM47_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1478649.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43177..44445)
                     /gene="folC"
                     /locus_tag="OQM47_000364"
     CDS             complement(43177..44445)
                     /gene="folC"
                     /locus_tag="OQM47_000364"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1478650.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44515..45429)
                     /gene="accD"
                     /locus_tag="OQM47_000365"
     CDS             complement(44515..45429)
                     /gene="accD"
                     /locus_tag="OQM47_000365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1478651.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45585..46244)
                     /locus_tag="OQM47_000366"
     CDS             complement(45585..46244)
                     /locus_tag="OQM47_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1478652.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46327..47139)
                     /gene="truA"
                     /locus_tag="OQM47_000367"
     CDS             complement(46327..47139)
                     /gene="truA"
                     /locus_tag="OQM47_000367"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1478653.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47139..48152)
                     /locus_tag="OQM47_000368"
     CDS             complement(47139..48152)
                     /locus_tag="OQM47_000368"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1478654.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48218..49354)
                     /gene="pdxB"
                     /locus_tag="OQM47_000369"
     CDS             complement(48218..49354)
                     /gene="pdxB"
                     /locus_tag="OQM47_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1478655.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49453..50448
                     /gene="flk"
                     /locus_tag="OQM47_000370"
     CDS             49453..50448
                     /gene="flk"
                     /locus_tag="OQM47_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1478656.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50445..51623)
                     /locus_tag="OQM47_000371"
     CDS             complement(50445..51623)
                     /locus_tag="OQM47_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1478657.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51878..53119)
                     /gene="fabB"
                     /locus_tag="OQM47_000372"
     CDS             complement(51878..53119)
                     /gene="fabB"
                     /locus_tag="OQM47_000372"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1478658.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53278..55284
                     /gene="mnmC"
                     /locus_tag="OQM47_000373"
     CDS             53278..55284
                     /gene="mnmC"
                     /locus_tag="OQM47_000373"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1478659.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55405..55683)
                     /locus_tag="OQM47_000374"
     CDS             complement(55405..55683)
                     /locus_tag="OQM47_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1478660.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55717..56265)
                     /gene="epmC"
                     /locus_tag="OQM47_000375"
     CDS             complement(55717..56265)
                     /gene="epmC"
                     /locus_tag="OQM47_000375"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1478661.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56265..57074)
                     /locus_tag="OQM47_000376"
     CDS             complement(56265..57074)
                     /locus_tag="OQM47_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1478662.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57074..57898)
                     /gene="mepA"
                     /locus_tag="OQM47_000377"
     CDS             complement(57074..57898)
                     /gene="mepA"
                     /locus_tag="OQM47_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1478663.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57902..58987)
                     /gene="aroC"
                     /locus_tag="OQM47_000378"
     CDS             complement(57902..58987)
                     /gene="aroC"
                     /locus_tag="OQM47_000378"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1478664.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59022..59954)
                     /gene="prmB"
                     /locus_tag="OQM47_000379"
     CDS             complement(59022..59954)
                     /gene="prmB"
                     /locus_tag="OQM47_000379"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1478665.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60120..60671
                     /gene="smrB"
                     /locus_tag="OQM47_000380"
     CDS             60120..60671
                     /gene="smrB"
                     /locus_tag="OQM47_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1478666.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60775..61665)
                     /locus_tag="OQM47_000381"
     CDS             complement(60775..61665)
                     /locus_tag="OQM47_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1478667.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61652..62176)
                     /locus_tag="OQM47_000382"
     CDS             complement(61652..62176)
                     /locus_tag="OQM47_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1478668.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62173..62643)
                     /locus_tag="OQM47_000383"
     CDS             complement(62173..62643)
                     /locus_tag="OQM47_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1478669.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62640..63188)
                     /locus_tag="OQM47_000384"
     CDS             complement(62640..63188)
                     /locus_tag="OQM47_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1478670.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63193..63915)
                     /locus_tag="OQM47_000385"
                     /pseudo
     CDS             complement(63193..63915)
                     /locus_tag="OQM47_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63935..64657)
                     /locus_tag="OQM47_000386"
     CDS             complement(63935..64657)
                     /locus_tag="OQM47_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1478671.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64717..65871)
                     /gene="yfcU"
                     /locus_tag="OQM47_000387"
                     /pseudo
     CDS             complement(64717..65871)
                     /gene="yfcU"
                     /locus_tag="OQM47_000387"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65820..66575)
                     /locus_tag="OQM47_000388"
     CDS             complement(65820..66575)
                     /locus_tag="OQM47_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1478672.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66657..67220)
                     /locus_tag="OQM47_000389"
     CDS             complement(66657..67220)
                     /locus_tag="OQM47_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1478673.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16838 a        18248 c        16753 g        15631 t
ORIGIN      
        1 agcatccggc aaaagagtga gttaacatca ttgttaatca attcgaccag tcagtaaata
       61 tgctgactgg tcgaacataa tttgctaaaa agtcatcact gactgatgtc agtcccacca
      121 tctgccagtt caatctacgg gtatttccag catatcgcgg cacgcgattg ctggtctgtt
      181 gatgtagtgg attgtagttg ctgacttttc ttaccctcag atttctgccc cacttgtcaa
      241 ttcaatctgc caataaataa cataattata tttttattcc tcacagaaat tcctttttaa
      301 aaatagcgaa cccactgtaa aaaacacttt ttgtatacct ctaccaagcg taataaaata
      361 aacgctcata attcattata aataataatg aaataacgcc ttttattgat aatttacata
      421 tgaaaaaatt ttcctttttt tatattattt ctgttttcac tccctctgat tatttttacc
      481 aaccctgggt cactgcatta tcgccaacgc cccgacacgt aagtcctgag caattagaaa
      541 aaacagttcg ttatcttaca caaactgtgc atccacgtaa tgccggcaat attgataatc
      601 tgaataggtc cgcagagtat ataaaagagt tctttgtcag tagcggtgcc agagttaccg
      661 cgcagggcgt ccccgattgg ctattacgac tctgcgcctg gaagccagaa ttacccttac
      721 ccggcaatgt cctggcttta tcccgatcgg ggagacttta ttgctgtggt cggcagaatg
      781 caggatatca acgccgttcg tcaggtaaaa gcggcattgt tgtcatcccg ggatttatct
      841 gtttattcta tgaatgcccc agggtttatt cccggtattg atttttctga tcacctcaat
      901 tattggcaac acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa
      961 caataccact tgcccggtga taccgcagac agattgaatt atcagaaaat ggctcaggtg
     1021 gtagatggtg ttataacctt gttatacaac agtaaataat aaaaatttct cgaggcgggt
     1081 ccagaaacag cgacatgtac attttatttt ccctcgtctg ttttaagatg cgaaacataa
     1141 aattcaatgg aggaaaaata aatgggaatg attggctatt ttgcagaaat tgactcagaa
     1201 aagattaacc aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg
     1261 ctttcaggat tacgccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc
     1321 attaagcgac tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag
     1381 gggcaactct ttgcctcgat catgcttgat atggaaaagc tgattagcgc gtatcgacgt
     1441 atgctgcgac aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagagcgcc
     1501 aaaaaaaacc gccgaacatg tcggcggctt tctgacttac aaactaacag tttacatcag
     1561 cggcattgct aaccgcacaa tactaatcag cggttgtggc catacaccca gcaccagtac
     1621 caacagtgca gaaatcagca ccacgatacc gcccgcgctg tactgccagt ttgacggtgc
     1681 atcgcgaccc ggttgttccg gtgcgtgcag gtacaggctc accgccacgc gcaggtagta
     1741 gtagaggccg attgccgaac cgacaaccac ggcacccacc agccaccaca agtgtgcctg
     1801 gacaccgacc gccagcacgt agaacttacc gataaagccc agcgtcatcg ggataccggc
     1861 cagagacagc atcatcaccg tcatcactgc cgcgaggatc ggacgatgcc agaacagacc
     1921 gcggtaagag aacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac
     1981 cacgccgaac gcgccgaggc tgctgaacag ataaccggcc aggtaaaccc ctaccgcttc
     2041 catcgacatc tcgccggttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga
     2101 gatagatgag taaccgagca gacgtttgat gttggtctgg ctcaacgcca tcaggttacc
     2161 gaagatgatg gaggcaaagg cgataatcgc cagtactacg cgaatcgctt cgctgtcacc
     2221 caccggtgcg tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt
     2281 cgccaggaaa gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg
     2341 gaacggcacc agagagagtt tgaagccgag gccaacaatc atcatgccga aacctgccag
     2401 caacagcggc tcgttgagca taccgtcgcc gaggttttta cccagcgcga caaacgacag
     2461 gtcgccagac tgcgcataca ccagcgccat accgaacagc aggaaagaag acgctgcggc
     2521 agaaaggatg gtgtatttga tactggcttc cagcgaacgt ttctggcgga aagcgtaacc
     2581 gaccaggcca aacagcggca aagagatcag ttcaataccg aggaacagag acgccagatg
     2641 gtttgcattc gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc
     2701 atccttgttg tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct
     2761 cgccaacaat accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt
     2821 aacgtccata gcgcccgcct ggccaacaaa ccagagcgaa accagcgccg cgttaagccc
     2881 aataaccgag agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac
     2941 aaccaccacc gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt
     3001 tattgtcatg gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat
     3061 attgccaatc gcggagtgcg aggtatccag aatcggctgc ggatagaagc ccagcagtac
     3121 cagcagcacc accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc
     3181 ctggctggca atctggcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga
     3241 ataaacagat gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg
     3301 gaagctgccg aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag
     3361 cgttgccacc gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc
     3421 gcccatcatg cgcatgtcgc gggtatggat acgttcataa agctgaccgc agagaataaa
     3481 cagacccgcc gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa
     3541 ctggctgccg gtgtagattg caatcagcac gaagcccatg tgggaaaccg aggtgtaggc
     3601 gatcagacgt ttgatatcgg tctgagcgaa ggtcatccac gcaccgtaga agatgccgat
     3661 aacacccagc cacatagcga ttggcgcgaa ctctgccgac gcgttcggga acagcggcag
     3721 ggagaaacgc aacaaaccgt aagcggcggt tttcagcaag atccccgcga ggtccacgga
     3781 accggcggtc ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg
     3841 cattttgact gcgaaggcga tgaagaagcc cagcatcaac agatattcca caccactgga
     3901 cattggcgta ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg
     3961 aacaaaaacc agcgccagga tggcgatcaa catcaccaga ccactcgcct gggtgtaaat
     4021 gaagaacttg gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag
     4081 tgcgatcagg aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat
     4141 gtcgatggca aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa
     4201 gaagccctga tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag
     4261 accggtcagc acgaccatca gcagcgacag accgtcaatg gcgagatgaa tagagatacc
     4321 aaaacgcggg atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg
     4381 cgtcagtgaa taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat
     4441 ggtgatcagc gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag
     4501 gaagccgcca ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc
     4561 ttgtaaaagt cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc
     4621 ggactggggg tagtgtcgga tgcgccgcga aggcagctta tccgacctac gttcttatat
     4681 gcccaggggc aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca
     4741 cgaccgcacc gatgctcatg gatgccacat accagcgcag ataaccgttc tcgcttaaca
     4801 gcagaccttt acctgcaaag cgggaaagaa ccgccgggat gttcatcatt gagttcagcg
     4861 gatcgcgttt cagcaaccag gcaataccca ggaacggctt gacgaacact ttgtcgtaca
     4921 gccagtcaaa gccccaagcg ttgtaccacc aggtactcag caggcggccc ggcgcactgt
     4981 tggcgatgga ggtcaccaga gaacgtttac ccagccacag ccaggctgcc agcagaatgc
     5041 cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt
     5101 ccgtcgtttg cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg
     5161 aaaggatcag cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt
     5221 gaatttgttc ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca
     5281 taaacgcacc gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg
     5341 cgaggatctc atccttactg aagaagcccg cagtgaccag cggcagtgcc gacagtgctg
     5401 cgccgcccac caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct
     5461 tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca
     5521 gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc
     5581 caagcgcgag gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga
     5641 tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatac
     5701 ccaccagatg cagaacttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt
     5761 agacacccgc ggttaccatc gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg
     5821 ccatcgcgtc agcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc
     5881 ccagcagcat cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg
     5941 ccagttccac catatcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg
     6001 cgaaagcgag gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gctgctgcgc
     6061 cattcttcgg atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt
     6121 cccagccgag gtacatcagc agcaggttat cggcgagaac cagaactacc atgctggcga
     6181 tgaacaggtt ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg
     6241 aggcgtacat gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca
     6301 ggccgtccag caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca
     6361 gcggctggct gtatgcctgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta
     6421 ccagcgccgc caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc
     6481 gcccacggga gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa
     6541 gcatgttcat ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg
     6601 aagttgcagc agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag
     6661 aatgtacatc acctgaccgt cggtctgacc ccagtagctt ccggcgacca caaaggccag
     6721 cgcggaggcg ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat
     6781 aaccagaccg gttaagccaa gaacgaataa gattgccgcg aggatcagtc catgttgtaa
     6841 ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc
     6901 acttcacccg cacgctcttc acgaccgacg tggaaggcca caaccagacc cgcgagcagc
     6961 agcatagaag ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct
     7021 ttagcactga ttggcgtacc gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca
     7081 atcaccacca gcatgatggc cgacaaaatt gccgggccaa tccacacctg cggtttcagc
     7141 cactggcgtt cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg
     7201 aacagcacca taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg
     7261 cctagtgaga agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg
     7321 tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag
     7381 atataaaaag cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag
     7441 gcttggcttc gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc
     7501 ggtagaagtt atattccggg tatttgcccg gaccggagat cagcagatcc tctttctcgt
     7561 aaaccagatc ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg
     7621 tggtcggaca ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttaatgcgga
     7681 aaaattccgg ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga
     7741 ccgggcaggc taccgcacag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc
     7801 gggtcagaac gatacgacca cgataacggg gcggcagata gaccggctct tccgggtaca
     7861 ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg
     7921 tgccgaaacc tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg
     7981 ccagagaatg acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt
     8041 ccagccgaag gacattaccc ggtcataacg cggacgcggt aacgacgcac gaatcaaaat
     8101 gaacatcatc ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg
     8161 gccttgccag ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat
     8221 gtactcaccc acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc
     8281 cgccagttcc tgctcggctt ccggctggtc aaacgggtga cggtgacaca ccgccacgcc
     8341 cgcgatggca aaggtaataa aaccaaagaa ttgcgggatg acgttccaca catgcgcctg
     8401 gctgttgacg atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga
     8461 aagcccgagg aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcag
     8521 cgagtatttg ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc
     8581 catcatcagg aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac
     8641 tggcacaatt gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa
     8701 gatgacgcga tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc
     8761 cgcaaccagc tggagcgaac cgccccagcc aacacggtta ggtccataac ggttctggaa
     8821 cagacccagc aggcgacgtt cgccaaagct catgaatgcc ccacaggtga caaccaccag
     8881 caggatcacc accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca
     8941 actcattgtt gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaatg
     9001 ccggacatac ccatcggcaa gcccacctgc cctgccgtca gtccttcggc gatttcaacc
     9061 ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc gtgtacctgc gttcacaccc
     9121 aacttcgcgg catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag
     9181 accggagcac gctgtgacaa ttcatcgctg ccaaacaggt ggtagtacgg cgcgatacgc
     9241 catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccgttt
     9301 tcgctggttt caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg
     9361 tcctggaatt tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact
     9421 tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca
     9481 atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg
     9541 ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc
     9601 gcagcatctt tgatacccgc cagttctggg attttcgcca caacagcgtc aattacatgg
     9661 tcgagctgcg tccagtccac ttcacggctc agcaaggtgc tgtgcagcga gtgcagccag
     9721 cgccagcttt ccagcatgac agttttgctg tcgtaataag ccggatcgta aacctggaag
     9781 aaacgttggg cgcgaccttc attattaatt accgtaccgt cgctttcagc aaagctggca
     9841 gaggaaagca ccagatgggc gttttccatg atcgctgtgc gttgatgatc aaccaccatc
     9901 accagcggcg ctttagccag cgcagcattc acgcgggtag cagaagcgta acgatgcaga
     9961 tcgttttcca acaccaccac cgcgtcggcg cgtccggttt ccagttcggt taacgcttct
    10021 tcaagcgaac cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg
    10081 gtgataccaa cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga
    10141 atcacctcta tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc
    10201 agtgcctgca cgatgacgtc gattttgttt tgcagctcag gttcgatacc gtcaaccgct
    10261 ggtgcggagt tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc
    10321 ggtgcgcggt aagtccacgc cgcgatatca tccagacgag tgtcatcaac gttggtaaca
    10381 aacagcggat gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc
    10441 actttctgtg ctgcagccat ttcgcgcgct ttacctttca ccgcctgacg cactgccagt
    10501 gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat
    10561 tcgatttcgc gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt
    10621 tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt
    10681 tcacgcagcg caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc
    10741 ttcgactgac gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg
    10801 aaatcatcgc cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg
    10861 taaccgaaac gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt
    10921 tcgatacgac gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat
    10981 tgctggcaga tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc
    11041 gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca ggttaccgga aaattcgctt
    11101 tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcgcc ataaacgccc
    11161 agatctgtac cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag
    11221 cggttcattt cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg
    11281 aaacggtagc gacggaagct gtgtccggtc atcacggtca tatcctgaag atggcagtta
    11341 ccgccttctt cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca
    11401 ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc
    11461 ggtgtcatac aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc
    11521 accgcacact ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga
    11581 atatcaaggc ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat
    11641 tctttgccgt ctacatgaat tgtagccatt agcatgcttc cagttttctc ggtcagggac
    11701 cgagcgttaa tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt
    11761 aatcaaatgg gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata
    11821 tttgatggcg ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc
    11881 cgggcctaag aatcgacaca gttgctcaag tgtttcgata tcgcccggct ggccttcgcc
    11941 acgctccagc gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca
    12001 ccagccgcag gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt
    12061 gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat
    12121 actttcgaat tccatcggca gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc
    12181 gcctggctgc caggctttaa atttcagacc atcacgcata ccaccggcgt aatcttcgag
    12241 gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc
    12301 ggagaagccc atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc
    12361 cacgccgtta gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt
    12421 cggtttgccc catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg
    12481 acgcccttcc agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc
    12541 ggtatggacg aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaagcc
    12601 cgcttcagtg gcttcggcaa tggcgcggcg cagatttact gccgcttcga tatattcgcc
    12661 acgcaggaag atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc
    12721 caccagcagg tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat
    12781 ttcatcggcg ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct
    12841 ccacttcagg ccagtcgaaa agcccgcgcc gccgcgccct ttcagaccag cgtcttttac
    12901 ctgattaacg atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc
    12961 gtttttgctg cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt
    13021 cagcggatgc gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca
    13081 gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat gtttggccct
    13141 ttatcacagt tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt
    13201 tgccctggtt tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccgttg
    13261 atatgacaga ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ctggcggaag
    13321 atctgactgt agaacgttgc cacaccttcg acgtcgcttg ccggaatacc cagcacatcg
    13381 gcgatcgcgt ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc
    13441 gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca
    13501 cgctctgccg cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt
    13561 agcggtccac atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca
    13621 ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga
    13681 tacgagtgcg ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct
    13741 tggtcgcctc aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt
    13801 gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg
    13861 tggtcagcgg gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa
    13921 gaatgcgcag actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag
    13981 aaacaccacc accaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg
    14041 ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact
    14101 ccagcgcctc tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat
    14161 tttgcagcgc cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac
    14221 gcagcagacg atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg
    14281 ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg
    14341 taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca
    14401 gcaggtggct gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat
    14461 ccggcacggt gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc
    14521 agccgccgag gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct
    14581 cgcccatttt ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct
    14641 cttcgccgtc gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac
    14701 cgaggttgag gaacatgaag tcctcatttt cggtgccgcg cttcatcccc cactcttccg
    14761 gtttgaaggt cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg
    14821 agaattcggt agcgcgtgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg
    14881 gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg
    14941 tttcacgctc ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt
    15001 cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat
    15061 ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt
    15121 ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc
    15181 caacttccag taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct
    15241 gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc gccaattacc ggatcatcaa
    15301 gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca
    15361 ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata
    15421 agcgaagaaa aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt
    15481 tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat
    15541 aaacgccctg atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct
    15601 gcaacagcat cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca
    15661 catcaaccgg gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac
    15721 cagagttggc acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca
    15781 gacgctgaat aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag
    15841 cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg
    15901 taaacgaagt caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg
    15961 agtttttacg accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa
    16021 cttcttgctc cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct
    16081 caccgttggg atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt
    16141 gacgattagc gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg
    16201 tccagtccag cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa
    16261 aaattgcagc ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga
    16321 acagatacag cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg
    16381 cggacaggcg taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt
    16441 tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca
    16501 ggccaatggc aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg
    16561 atgttgacat actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg
    16621 cagatggacg ccacatcgat tcatggggag gaataaaaaa aaccttacaa taactgtaga
    16681 aattctttta tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac
    16741 tttaacaaaa gcttcttcac attagtttac ataatatcaa caccattagc atttaatgcc
    16801 ctttcacccc agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt
    16861 gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc
    16921 caaaagagag tattttcttg atctgtgaca cgcttttgtt attccataac aaaaacgcag
    16981 caacaaattt acgtattttt taacatcatt gtagcaggtg atttttttca ggcgattatt
    17041 tgtgcattcg ggacgtgaat ctctggtgat tgaaaaataa acagttttgt acgttctgca
    17101 ctatgcgagg aaggattttt actaaaaaaa agccgctggg gtttaaaaca cccccagcgg
    17161 ctcgtttttt acactattgt ctcaggaatt atctatcgtc cgtcgattac tcgatatccc
    17221 tttcaatcaa caatgaatca tccccttccg gagcagacat tgggctgtat tgccacggat
    17281 tatggtagct ctccatcgcc tgatagatca cctgggccag ctcattatta ctggacgggt
    17341 catagcacag caaatattcg gtatcaggta gcggcggtaa gccatctact ccactcagca
    17401 cacgcaggtc cgggctcatc atctcaaccg gccttgccgt cacgccaaga ccggctttca
    17461 ctgccgcacg aacggccgga agcgtcgagg cgacataagc cagtcgccat ggaatatcta
    17521 ctttattaag cgtcgccagc accatatcgc gaaacggact tggatcatcc agcaatacaa
    17581 gagggatcgg ctcgcctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg
    17641 aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc atcaaatcca
    17701 cttcctggga ttccagcatt tctgccatat aggcattacg tttaacgcgg acatccagcg
    17761 ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag
    17821 attcatctga agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc
    17881 aggcctcatc attaaaacgc aggattttcc tggcgtaacc aagaagttga atgccatgtt
    17941 cagttaacag tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca
    18001 gacgctgcat ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag
    18061 caaaagtgtt cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa
    18121 ttatcggacg atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg
    18181 ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc
    18241 atccggaacc cggacgaaag taaaaatgca tatgagttgc actaaaaaag cgactcacat
    18301 tgttccgtta taatgcctga agtagatcac agaatatatc ttcagggatc gcatatctat
    18361 taagttactc actcttttct atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag
    18421 tccttgttcc actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg
    18481 gtgaagcggc atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct
    18541 tttatttata agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt
    18601 aatcttctat tattaataag cacatcaaaa cctttttgaa tattaaataa taattaatta
    18661 gcatcatcct cattcattaa ttccgcttaa caatagtttc acaccttacc cccattgcga
    18721 cacctcggaa atcatcacgc tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt
    18781 tcattagtga attcttaaat gaggcatttt cacacaatta tcttacagat aaaaaaacca
    18841 gacttgcaat taagaattag aacaattcac tatataacat tgcatgtaaa gcatataagc
    18901 ctcattattt gtcattatta agttattaac agcacaatcg agccttcccc tctggcaaaa
    18961 tcttattctg cagaccttca aaacaccgtc ctgggggagt acattgttct aagctgactt
    19021 ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa
    19081 atccagcaaa ttagagaatg tctgttatga catccgtggt ccggtgctga aagaagcaaa
    19141 acgcctggaa gaagaaggta acaaggtact gaaactgaac atcggcaacc cagccccatt
    19201 cggttttgac gcgccagatg aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca
    19261 agggtattgc gattccaaag ggctttactc cgcgcgtaaa gccatcatgc agcactacca
    19321 ggctcgtggc atgcgtgatg ttaccgtgga agatatttac atcggcaatg gtgtatcgga
    19381 gcttatcgtt caggcaatgc aggcattgct gaacagcggg gacgaaatgt tggttcctgc
    19441 accagattac ccactctgga ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta
    19501 tctttgcgat gaatcctctg actggttccc ggacctcgat gatattcgcg ctaaaattac
    19561 gcctcgtacg cgtgggattg ttattatcaa cccaaataac ccaaccggcg cggtatattc
    19621 caaagagctt ttaatggaga ttgtggagat tgcacgtcag cataatctca ttatcttcgc
    19681 cgatgaaatt tatgacaaaa ttctctacga cgatgctgag catcactcaa ttgcgccgct
    19741 ggcacctgac ctgctgacca ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg
    19801 cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga
    19861 aggtctggaa atgctggctt caatgcgcct gtgtgctaac gttcctgcgc aacacgccat
    19921 tcagaccgcg ctgggtggtt atcagagcat cagtgaattt attacccctg gcggtcgtct
    19981 ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat attccgggcg tttcctgcgt
    20041 gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac gccaaacgct ttaacattca
    20101 tgacgatcag aaaatggtgc tggatttcct gttgcaggaa aaagttctgt tggtgcaagg
    20161 gacggcattc aactggccgt ggccggatca cttccgcatt gtcacgctac cgcgtgtcga
    20221 tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt tctggttatc atcagctgta
    20281 atcttaattt cactgccgga gattgcatcc ggcagtgtta tcccgccaca atgacctgat
    20341 gatgtcatca tacgtaaggt cactatgaaa cagagccatt tttttgccca tctctcccgc
    20401 ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa
    20461 cacagtttgc aggtagcgat ggtcgcccat gcgctggcag ctatcaaaaa tcggaaattt
    20521 ggcggtaatg tcaacgccga acgtatcgct ttactggcga tgtaccacga tgcctcagaa
    20581 gtgctcaccg gcgatctccc tactccggtg aaatacttca atgcgcaaat cgctcaggag
    20641 tacaaggcta ttgaaaaaat cgctcagcag aaactggtcg atatggttcc ggaagaactg
    20701 cgggatatct ttgcgccgtt aattgacgag catgcatata gcgatgaaga aaaatcgctg
    20761 gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga
    20821 aataatgaat tcttgctggc aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc
    20881 caggagatgg actacttcat ggaagtattt gttcccagct tccatctttc gctcgatgag
    20941 attagccagg attcaccgct gtaagcagcc ggagtctgcg tcgcatcagt caataagcgc
    21001 cggatgcgac atcaggctct tgtcaaaacg gaaacagcat cgggatcatc accacacaaa
    21061 ccgccatcac gataatggtg aacggtaccc ccaacttcac aaagtcactg aagctgtaat
    21121 ttcccggacc taaaaccagg gtgttaacag gtgaagacac cggtgtcata aaggcggcgg
    21181 atgctgccat cgccacgacc atcgcaaatg gataaggcga cactcccatc gttttggcag
    21241 cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt attagagata aacagcccaa
    21301 taaccgccga caagacaaac aaacacccca gcatcatatg tggcccataa ccgccgccaa
    21361 tatccatcag ccctttcacc gccagcgcga cacctcccgt tttctgtaat gccacagcaa
    21421 acggcatcat cccaacggtc aaaataatgc tcggccagtg aatggattta taggcgcttt
    21481 cagcatctat acagcggaat ttccccatca gcaggcaggc gatgatagcg gcaacaggat
    21541 taggaatttc atttgtcagc attaacgcca ccattagcac cagacagaaa atggcatggg
    21601 gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc cggtaagttc agcgctacga
    21661 agtcgcggcc ctgtttggcc agcataccga tcagtttcca gttacccaca accaggatga
    21721 tatcgcccag cagcagaggc tcatccgcca gcgaaccttc cagcgccacg ccattgcgct
    21781 tgagccccac cacattcagt ccgtagcggg tacgaaaacc aatttcgcgc accgatttac
    21841 caatcagttc tgactcagga attaatgaaa tctctgccat gcccacatca agggcctggt
    21901 cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa ttgctcacta caaaattgcc
    21961 ggagatcgac atcagccgca gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag
    22021 aaaccccatt aacgttcacg ataacgcgac gaaaacgccg ccagcgttca acaccgatga
    22081 cgttagcgcc ataacgctca cgtaatttga gatcatccag ccgttgacct atcattggcg
    22141 atccggggcg aatagccaga cgtcgcgcac gcccggtcag tcgatattca cggataagat
    22201 cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt ctgggtatcc cctttcagca
    22261 tgaaacgcat cactaacata tacaaaatac ccagcaccag cacgaccagg ccaataggtg
    22321 ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag caattcactg ttgactacca
    22381 ggttcggcgg cgtcgccacc agcgtcatca tgccgctaat cagcccggca aaacttaacg
    22441 gcatcatcag acgtgacggc gaagtttgca tacgcatggc aacgcttaac accacgggaa
    22501 taaagatagc gacaacgctg gttgaactca taaacgcgcc aagccccgcg acggtcagca
    22561 tcaacaaaac caacattttg atttcactgt tgcccgccac tttgaccagc catgttccca
    22621 ttacggtggc aacaccggta cggaccaaac catcgccaat aataaacaag gcggcaatca
    22681 ggacaacgtt aggatcagaa aagccggaaa atacttcggg gaccgtcagc gttccgctta
    22741 atgcaaacgc gacaataaca aacaaagcga ccgcatccat acgcactctg cccgtcgcaa
    22801 acaagacgat ggcaacggcc agtaatgaaa gaacccaaat caattcaccg ttcacaactt
    22861 atccttgtta attgaggggg atgacttgat tctgccataa aaaagccccg acgagacggg
    22921 gctaaatcat gatcaggtgt ttcactgaat aataacatcg ccatttggct gtttggtcac
    22981 agtaatttgc tccagactgt ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc
    23041 cggagcgtta accgcaatga catggcaacc cgccgccagg ccagaaagca cgccagcggg
    23101 agcatcttcc accaccacac actcctgcgg cgcaagcccc agcagctgcg cacctaacag
    23161 atacgcatca ggttctggtt ttccgcgctt cactcgctca gcggttacaa acacctctgg
    23221 tgcgggaagc cccgctattt tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac
    23281 aatggcccac ggaatacctg ctttattcaa atgattgagt aaggcgattg ccccaggaag
    23341 cgcggtaata ccttcggttt ccgtggcctc gatttgctcc agacgcgtaa actcggcggc
    23401 aatatcagcc tcggatttgc ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc
    23461 gtgaatgaaa gccagcacct cttccggcgc taacccatga cgtctggccc agttgctcca
    23521 cgcccgttct accgcaggca gggaatccac cagcgttcca tcaagatcaa acagaaaacc
    23581 tttgcaccgc acgcgggcct cctcaggcgt tgataatttg attaatttcg ttggcgctca
    23641 aatggtactg acgcgggcag gcatgccaca cattaagcat gcgctgatat ttttcccaca
    23701 ttggcgtctg ggcgttaaaa ccatgagtcc cagcgtcaaa atgggtatag cgcccttcca
    23761 cattaaccat aaagcggaca taaccgaggt aacgtgcttc agtggcggcg tcaaagccga
    23821 ggaaggtgac acgacgttca tcgatggatt gctggtcctg caaattagac caggaaacat
    23881 acaacgcatg atacatatcc ataatgtcga tgatagtgcg gcaggtttct tctttcagct
    23941 cgccaaactc gcgatccaat tcacgcatct gtaatccgta accacgctca ataattgttt
    24001 gcaggcgacg gtaacgttca gcatttgccg gatcgagcat agtcatcatc ttgtactgat
    24061 tagacaaaat aagacgttgc gcgttggtca tttccattgt tgactcctgt atcactctac
    24121 tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat atgcgtaatc aggggtcaat
    24181 tacaaatcat caaggaaagt tttatccagt tgtttgaagg cgcgcttaag cgtgtcagct
    24241 aatgcctggt aatcaggctt gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat
    24301 ttaccgcgaa cttcataaaa ccagttaagg attgcagggg gtaatggcgt tacagaacgc
    24361 ttgcccagcc accacaatcc ctgcatgggt aaacttaagg cgaacagtgc agtggcaact
    24421 gccggcccaa gcagaccgcc cagggcaatc tgccagcaga gagtaaatac ggcgatcggc
    24481 ggcataaaac ggatcgcata acgcgtcatc ttgataacgc gattttcgac aaagaccgga
    24541 gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct gtccgcggcg aaacaagcta
    24601 aaaaaattaa cagaacgatt atccggcgtt gacatgcttc acctcaactt cacatataaa
    24661 gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa cgttcaaaac attttgtctt
    24721 ccatacccac tatcaggtat cctttagcag cctgaaggcc taagtagtac atattcattg
    24781 agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat tacgcaaaat ggcatagact
    24841 caagatattt cttccatcat gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt
    24901 cggtgtcatc atgcgctacg ctctatggct ccctgacgtt tttttagcca cgtatcaatt
    24961 ataggtactt ccatgtcgag taagttagta ctggttctga actgcggtag ttcttcactg
    25021 aaatttgcca tcatcgatgc agtaaatggt gaagagtacc tttctggttt agccgaatgt
    25081 ttccacctgc ccgaagcacg tatcaaatgg aaaatggacg gcaataaaca ggaagcggct
    25141 ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta tcgttaatac tattctggca
    25201 caaaaaccag aactgtctgc gcagctgact gctatcggtc accgtatcgt acacggcggc
    25261 gaaaagtata ccagctccgt agtgatcgat gagtctgtta ttcagggtat caaagatgca
    25321 gcttcttttg caccgctgca caacccggct cacctgatcg gtatcgaaga agctctgaaa
    25381 tctttcccac agctgaaaga caaaaacgtt gctgtatttg acaccgcgtt ccaccagact
    25441 atgccggaag agtcttacct ctacgccctg ccgtacaacc tgtacaaaga gcacggcatc
    25501 cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa cccaggaagc ggcaaaaatg
    25561 ctgaacaaac cggtagaaga actgaacatc atcacctgcc acctgggcaa cggtggttcc
    25621 gtttctgcta tccgcaacgg taaatgcgtt gacacctcta tgggtctgac cccgctggaa
    25681 ggtctggtca tgggtacccg ttctggtgat atcgatccgg cgatcatctt ccacctgcac
    25741 gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg
    25801 ctgggtctga ccgaagtgac cagcgactgc cgctatgttg aagacaacta cgcgacgaaa
    25861 gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc tggcgaaata catcggtgcc
    25921 tacactgcgc tgatggatgg tcgtctggac gctgttgcat tcactggtgg tatcggtgaa
    25981 aatgccgcga tggttcgtga actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt
    26041 gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt
    26101 acccgtcctg cggtggttat cccaaccaac gaagaactgg ttatcgcgca agacgcgagc
    26161 cgcctgactg cctgatttca caccgccagc tcagctggcg gtgctgtttt gtaacccgcc
    26221 aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat tattatgctg atccctaccg
    26281 gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag
    26341 gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg taccggtggc gatgcgcccg
    26401 atcagactac gactatcgtg cgtgcgaact cttccaccac gacggccgct gaaccgctga
    26461 aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa agatgtgctg atggaagaga
    26521 tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt cgttctggtt gaaggtctgg
    26581 tcccgacacg taagcaccag tttgcccagt ctctgaacta cgaaatcgct aaaacgctga
    26641 atgcggaaat cgtcttcgtt atgtctcagg gcactgacac cccggaacag ctgaaagagc
    26701 gtatcgaact gacccgcaac agcttcggtg gtgccaaaaa caccaacatc accggcgtta
    26761 tcgttaacaa actgaacgca ccggttgatg aacagggtcg tactcgcccg gatctgtccg
    26821 agattttcga cgactcttcc aaagctaaag taaacaatgt tgatccggcg aagctgcaag
    26881 aatctagccc gctgccggtt ctcggcgctg tgccgtggag ctttgacctg atcgcgactc
    26941 gtgcgatcga tatggctcgc cacctgaatg cgaccatcat caacgaaggc gacatcaata
    27001 ctcgccgcgt taaatccgtc actttctgcg cacgcagcat tccgcacatg ctggagcact
    27061 tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc tgacgtgctg gtggccgctt
    27121 gcctggcagc catgaacggc gtagaaatcg gtgccctgct gctgactggc ggctacgaaa
    27181 tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc taccggcctg ccggtattta
    27241 tggtgaacac caacacctgg cagacctctc tgagcctgca gagcttcaac ctggaagttc
    27301 cggttgacga tcacgaacgt atcgagaaag ttcaggaata cgttgctaac tacatcaacg
    27361 ctgactggat cgaatctctg actgccactt ctgagcgcag ccgtcgtctg tctccgccag
    27421 cgttccgtta tcagctgact gaactggcgc gcaaagcggg caaacgtatc gttctgccgg
    27481 aaggtgacga accgcgtacc gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa
    27541 cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag
    27601 aactaggtgc aggcattgaa atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc
    27661 gtctggtcga gctgcgtaag aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg
    27721 aagacaacgt ggtgctcggt acgctgatgc tggaacagga tgaagttgat ggtctggttt
    27781 ccggtgctgt tcacactacc gcaaacacca tccgtccgcc gctgcagctg atcaaaactg
    27841 caccgggcag ctccctggta tcttccgtgt tcttcatgct gctgccggaa caggtttacg
    27901 tttacggtga ctgtgcgatc aacccggatc cgaccgctga acagctggca gaaatcgcga
    27961 ttcagtccgc tgattccgct gcggccttcg gtatcgaacc gcgcgttgct atgctctcct
    28021 actccaccgg tacttctggt gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc
    28081 tggcgcagga aaaacgtcct gacctgatga tcgacggtcc gctgcagtac gacgctgcgg
    28141 taatggctga cgttgcgaaa tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg
    28201 tgttcatctt cccggatctg aacaccggta acaccaccta caaagcggta cagcgttctg
    28261 ccgacctgat ctccatcggg ccgatgctgc agggtatgcg caaaccggtt aacgacctgt
    28321 cccgtggcgc actggttgac gatatcgtct acaccatcgc gctgactgcg attcagtctg
    28381 cacagcagca gtaatctcgt catcatccac agctttgcgc tgcggatatc tgaaccggaa
    28441 ataatcacta tttccggttt tttattctct taatttgcat taatcctttc tgattatctt
    28501 gcttaactgc gctgcatcaa tgaattgcgc catctcactt tgcatactta ccactttgtt
    28561 ttgtgcaagg gaatatttgc gctatgtccg caatcactga atccaaacca acaagaagat
    28621 gggcaatgcc cgatacgttg gtgattatct tttttgttgc tattttaacc agtcttgcca
    28681 cctgggtagt tccggtgggg atgtttgaca gtcaggaagt gcagtatcag gttgatggtc
    28741 aaacaaaaac acgcaaagtc gtagatccac actcatttcg cattctgact aacgaagcag
    28801 gcgaacctga gtatcaccgc gtacagctgt tcacgacggg cgatgaacgc ccgggcctga
    28861 tgaacttccc gtttgaaggg ttaacctcag gatcgaaata cgggacagcc gttggcatca
    28921 tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg
    28981 ataacggtat cctggcgctt attcgccata cccgcgggaa tgaaattctc tttattcctg
    29041 cgctgtttat tctgttttca cttggcggcg cggtatttgg tatgggagaa gaggccgtcg
    29101 cctttgccat tatcatcgca ccgctaatgg tccggctggg ctatgacagt attaccaccg
    29161 tcctggtgac ttatattgcc acgcaaatcg gttttgccag ttcgtggatg aacccgtttt
    29221 gtgtggttgt tgctcagggg attgccggcg ttccggtgct ttctggctcc gggttgcgca
    29281 tcgtggtgtg ggttatcgcc actctgattg gcctgatctt taccatggtg tacgcctcac
    29341 gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa
    29401 agcaggcgga tgttgaacaa cgtccgttta cctttggtga ctggctggta ttgattgtcc
    29461 tgacagccgt aatggtctgg gtgatttggg gcgtgatcgt taatgcctgg tttattccag
    29521 aaattgccag ccagttcttc accatgggtc tggtgattgg catcatcggc gttgttttcc
    29581 gccttaacgg catgacggtt aataccatgg cttcatcctt taccgaaggg gcgcgaatga
    29641 tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat tttgctgctg gttggtaatg
    29701 gtgaagcagg taatgccagc gtgttaaata ctatcctcaa cagcattgcc aatgccatta
    29761 gcggtctgga caacgcggtc gcggcctggt ttatgttgct cttccaggct gtatttaatt
    29821 tcttcgtgac gtccggttct ggtcaggcgg cattaactat gccgttactg gcaccgcttg
    29881 gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc gttccagttt ggtgatggct
    29941 tcagccacat catttaccca acctcagctt cgttaatggc aacgctcggt gtgtgccggg
    30001 tggacttccg taactggctg aaggtgggcg cgaccctgct tggactgctg tttattatgt
    30061 ccagcgtcgt agtgatcggc gctcagttga tgggctacca ctaaaaatgt taagagccgc
    30121 aattgcggct cttttttcat tctgccgttt cagtctctac ggcttcattt ttggcattgc
    30181 gcttcatcca caacgccagc gctttcagcg agtctggagt gaactcatcg cagcgtgcgg
    30241 tgatttcttc cggcgtcagc cagcaaactc cactgacttc atcttcctgt agggcgaagg
    30301 gaccgtgaga gacgcaactg aacaacgcgc cccagacacg gcaattttta tcttcgaaat
    30361 agaactgccc gtgctcggca aaggggacac cggcaatgcc caactcttct tccgcttcgc
    30421 gacgcgcgga ttccagcagt tgctcatcgg cctgaactac accgcctgcg gtcgcatcta
    30481 acatgccggg taaaaagtct tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc
    30541 catcatgcac gacgatgtaa gttgcacgat gacgcagaca ctgtgcccgc atttgttccc
    30601 ggctggcttg tgcaatgact tcgttctctt cattgacaat atccacccat tcagtacttg
    30661 ccaaacgacg ctgttccatt atcaggaaac cttctttttc tggcgctctt acggcgcatt
    30721 ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc aatgatactt tgatcattga
    30781 gtgcgattac actaagtacg tcattatcca gcatgccata acccgccgga ttaccgcctt
    30841 tcggaatact caccgagccg gggttgaagt ggaaaatctc cccccgctgt tctgccactg
    30901 gcagatgggt atgaccgtac accagcacat cgttctggtt taaagcaggt agattttccg
    30961 ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc
    31021 acggcgcggt tatcgggaaa tgcagcatca tttgatccac ttcgctgtcg cagttgccac
    31081 gcacagcgat aaccttatgt gccacttcat taagccgttc agcgactttg gctggcgcgt
    31141 aaccctccgg taaagcatta cgcgggccgt gattcaacac gtcgccaagg atcaccagcc
    31201 actgggcacc gctttggaca aacaactcca caacacgttc cgtcgccggt aacgacccat
    31261 gaatgtccga tgcaaacatc agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat
    31321 gatactggat tctgtcgttg ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc
    31381 tggacagacg ctcgctgcca ctggaatgtc gcataatcca ccaggcgttc tggcacctca
    31441 tcgccatgta gtaccaggcg gttaatcatt agcgccagat cagtatcagc aatgcaccat
    31501 tcaccaaata aattcggctg acccagtgct aacaaatgtt ctgccatcgc gaacagtttc
    31561 tctgcactgg cttttccctc ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca
    31621 tccgtcggac gctcttcgcg gatgggcatc agatcgctgc gcagccaggc ctgaatctgt
    31681 cgtgcacgcg cacgattttc taaatcaagc ggataaatac gttcccaggt cggtggcgca
    31741 aatcgatctt ccagatactc cgcaatggca gaagattcac tcaactcaaa atcatcgatt
    31801 tgtaataacg gcacacggcg tgtctgaccg taaccttgcc acgtcggctg caaatgttca
    31861 ccgctgtcga ggtcgatggt cttgatatga aacgacaggc ctttttcctg caacgccacc
    31921 caggcggata acacataagg ggagaaaaag tgggcatctg accaaagcgt gatagcgggt
    31981 ttactcatta tgtcctcggc aataaagggg gctggctatc aaactataga gcctttttgt
    32041 cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt tctatacttg tggtgttcag
    32101 atgattaaac aaccggagct gcaatgatcg atctctattt cgccccgaca cccaatggtc
    32161 acaaaattac gctgtttctc gaagaagcag agcttgatta tcgcttgatt aaggtagact
    32221 tggggaaagg cggtcagttt cgtccggaat ttttgcgcat ttcgcccaac aacaaaattc
    32281 cggcaattgt tgatcattct cctgccgatg gcggcgaacc gttaagcctc tttgagtctg
    32341 ctgccatttt gttgtatctg gctgagaaaa caggactctt tttgagtcat gaaacgcgtg
    32401 agcgcgccgc cacattacag tggttattct ggcaggtagg cggactgggg ccgatgcttg
    32461 ggcaaaatca tcattttaat cacgcagccc cccaaaccat tccttacgct attgaacgtt
    32521 atcaggttga aactcagcgt ctttaccatg tactaaacaa gcggctggaa aactcgccct
    32581 ggctgggagg cgagaactac agcattgcgg atattgcctg ctggccgtgg gttaatgcct
    32641 ggactcgcct gcgaattgac ctcgcaatgt atccggcagt caagaactgg catgagcgga
    32701 tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc acaactcggt gatgagcgtt
    32761 cggatagtta acagaaacag gttctcgtgt attatttcat cctaagtaaa acaacggaga
    32821 acctgcaatg gcacaacctg ccgctattat tcgtataaag aaccttcgtt tgcgtacgtt
    32881 tatcggaatt aaggaagaag aaattaacaa ccgtcaggat attgttatca atgtgacgat
    32941 ccactacccc gccgataaag cgcgcactag cgaagatatc aacgatgcgc tgaattatcg
    33001 caccgtaacg aaaaacatta ttcagcatgt agagaataac cgtttctctt tgctggaaaa
    33061 attaactcag gatgtgctcg atatcgcacg tgaacatcac tgggtgacgt atgctgaagt
    33121 ggagatcgat aaactgcacg cgctgcgcta cgccgattcg gtatccatga ccttaagctg
    33181 gcagcgttaa tcgccatatc gggaggctgc atgaacatag tgatcaccgg agggacggga
    33241 ttaattggtc gccatttgat tccgcgtttg ctggagctgg gccatcaaat tacggtagtg
    33301 acgcgtaacc cgcagaaagc cagttccgtt ctcggccctc gggtgacact atggcaaggg
    33361 cttgccgatc aaagcaacct caacggcgtt gatgcggtaa tcaacctggc cggagaaccg
    33421 attgctgata aacgctggac tcacaagcaa aaagagcgtc tctgccaaat ccgctggaat
    33481 atcacgcaaa aactggtcga tttgattaat gctagcgaca cgccgccgtc ggtactcatt
    33541 tccggctcgg caacgggcta ttatggcgac ttaggtgaag tggtggttac cgaagaggaa
    33601 ccgccgcata acgaatttac tcataaactc tgcgcccgct gggaagagat tgcctgccgg
    33661 gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgaaccg gcgtggtact ggcaccggac
    33721 ggcggtattc tcggtaaaat gctgccaccg tttcgtcttg gcctgggcgg gccgattggt
    33781 tccggtcggc agtatctggc ctggattcat attgatgata tggtcaacgg cattctctgg
    33841 ctgctggata acgagctgcg cgggccattt aatatggttt cgccctaccc ggtacgcaat
    33901 gaacaatttg cccatgcgct cggtcatgcg ctgcatcgcc cggctatttt gcgcgtcccc
    33961 acaaccgcca ttcggctgtt aatgggcgaa tcttcagtac tggtattagg tggacaacgc
    34021 gcgctgccta aacggctgga agaagcgggt tttgcgtttc gctggtacga tttagaagag
    34081 gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa catccgccag ttaactccct
    34141 gtgttacagg attagtggct ttgcgcgata agatcgtctg gtgaaagtcg ggtcaccatc
    34201 ataactaact ctctgtctaa acctctatcc agcatctcct gagcaatacg cagggcttct
    34261 tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt cagcaatggt catcagtttc
    34321 tccttttctt gtggtgcgcg ttctgttatc tcaccaataa atgcacggaa acgctgggca
    34381 tcccctgttt gtaatacgta attaaacagg gcttttagct gtctgtcatt agtgttccct
    34441 gtaactagca gcgaaacaat ttggtcgact aatcccaaca gatcgcgctg acgaatatgt
    34501 ttctgaatta actccaacag cgccattttg cggtgttgca taatctcgtc atccggcacc
    34561 acggtaatat ccaccaacgg aaacgctgat gaatatattt tgcgggctat agcaggctcg
    34621 gcaaattcat caagccagca gagtgaataa ggataaggac ttctgcaacc atgataaaac
    34681 agcatcggga tcaccaatgg aagctcttta tagcccgcat caagatggtt ttgcattgcc
    34741 gcaatggaat aacgcatcat gcgaaaagcc attaattctt ccggcttact ttggtgctct
    34801 atcactacat aaatataacc cactccctcc tgcgttttca cagaccacaa gaggtcggaa
    34861 taatattgcc gcaggtcttc atcaataaaa ctgtttggtt ctagtttaag cgtcgttaaa
    34921 tcacacagtt tgcgcagcgg cgcgggaaga tgaatattaa taaaattccg cgcggtgtct
    34981 ggatggcgta aaaaagattt aaataccgca tcatgcggcg tggaagttgt cgatattgtc
    35041 atggcgttcc gtcacccctc aaatttaaga tgacgcgaca ataaccacga ttaacaccca
    35101 atgcagcagg cagtttttat ctttggacag cgaattcaga agtattttta gcggactgca
    35161 acttttacat ttcagcggaa gctgactcgc aaattcccct tgaaaatact ccggcagcgt
    35221 tagccaccgg agaggaaatt atttcagcga tcccttaagg aactgttgca gacgagggct
    35281 ttgcgggttg ccaaataact gctccggcgc gccctcttct tctattttcc cctgatggag
    35341 gaaaatgacg tgagtagaaa catggcgagc aaagcccatt tcgtgagtca ctaccaccat
    35401 ggttttcccc tcttctgcca gttgctgcat aatacgtaac acttcgccta ccagttcagg
    35461 atcgagcgcc gaggtaggtt catcaaacag taaaacttct ggttccatcg ccagcgcccg
    35521 cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc accggatatt tcccctgcgc
    35581 acgttcgtct atcccgactt tcgccagata cttcaccgcc cgctcgcgcg cttcctgctt
    35641 gctcaggccc aacacctgaa tcggcgcttc catgacgttt tccaacaccg tcatatggct
    35701 ccagagattg aagtgctgga ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt
    35761 tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc agattgatcg tctggccatt
    35821 gaccacgatc gatccttcac tcggtttttc gaggaagtta atgcagcgca gaaaggtact
    35881 tttccccgat cccgacgatc cgatgatgct tattacatct ccggcattcg cttgcagtga
    35941 gacccctttc agcacttcgt gttcgccata gcgtttgtgc aaatcgataa cgtttaattt
    36001 attctcggac atcgtattct cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt
    36061 tttccgctct gcgaaagagg ctgatcagga cataagagat gattaaatag agcaccgcgg
    36121 caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt aatatcgcgg gctattttca
    36181 gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga gtgcagcatc aggatcactt
    36241 cgttgctgta tgccggtaac gcaatacgca gcgcagaagg caaaataatg cagcgataca
    36301 ttttaaaggt cgagaagcca taggctctgg cggcttcaat ttccccatgc ggaaccgaac
    36361 ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca
    36421 cggtacagtt caggccactg cggaagaaag cgttaaggaa ttcggttccc ttaacaatct
    36481 caagcgtgta catgccggaa tagaacacca gcaactgaac atacagcggc gtaccgcgaa
    36541 aaatataggt aaataaccag attggaaact ggatgtattt attactggag acacgaccaa
    36601 tcgccagaaa cagcgccagg actccgccta tcactaccga caaaataagc agccacagag
    36661 tgatcgccac accagtaaag cgataaccgt cggtccacag cagcggtttc cagtattcat
    36721 gtaagatttc gatcacaggt cagccctctt cacacccacg gagtagcggc gctcaaggaa
    36781 cagcagcaca ccattggaaa cggtggtgaa aaccaggtaa atcacgccac agacgatggc
    36841 gaaatagaac ggttcccagg tacttttgcc tgccagctgc gtggctttga ccacatcttc
    36901 caggccgagt aacgaaacca gtgcggtaga tttgaggatc acctgccagt tgttgccaat
    36961 gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc cgccgaaaca cttgcccgcg
    37021 agtaaaaccg aacgccgtcg ccgcctctat atgccctttc ggcactgcca taaacgcgcc
    37081 acggaacgtt tcggtaaaat aagcaccgta gataaaaccg agagtgataa taccagcgac
    37141 catcggatcg atatcaatct gcccgacgcc catcgcctcc gtcaccatgt ttagcgcaat
    37201 ctgcaaacca tagaaaatca gcagcatcaa caccaagtcc ggcacgcctc gaatcagcgt
    37261 ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc gagagcttac cgccagcgcc
    37321 aattaaaccg atgattacag cgagcactac agagctgata gccagctcca gcgtgacgag
    37381 cgcaccctgt aaaataacac ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt
    37441 ggtgaattac cgcctcttgt ctccctccgg ttacccgaag ggagcgggct ttctttcacc
    37501 gatggcgatt aaccaccata aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa
    37561 gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg cctcgcgcag ttcgttatct
    37621 tctttacgca ggcccatgcc ggtccctacg ccaaacagtt tttcatcttt aacagacgga
    37681 ccaccgaatt tgtaatcttt accgacgggt tgtttgagga aaccttcgct ggcagcgacc
    37741 tcatcctgga acgcggcatc aatacgtccg gcagtcaggt cagaataaat gttgtcctgc
    37801 ccctgatacg agacgatttc aatgcctttt ggtgcccagt gttcattacc gaacgtctcc
    37861 tgggtggtgc cctgcaatac gcctacccgt ttgcctttca gcgactcgac tgtcggctga
    37921 atgtcagaat ttttcgccac caccagacga gaatcggcag cgtacagttt gtcggtgaag
    37981 gcaatttctt gctgacgttt ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc
    38041 ttcgctttta aggacgggat taacgcatcc agcgggtttt cgacaaacgt acattgcgta
    38101 ttgatgcgct tgcacaactc cttcgccagc tcgatatcga aaccaaccag ttcgccttgt
    38161 gaattttttg attcaaatgg cgcataggtc gggtcggtac cgatgcggat gttttgcgga
    38221 attgcagcaa gtgccgcagt tgcgctggag aaggccagaa ccagagagag cgatagcacc
    38281 agttttttca taatgtcctc aactgacagt ctttttatag ggaatattta caggtttgtt
    38341 acacttatcg tgccataaaa ttgaccatca aggcaaaata tcccgccccg ttagacgtat
    38401 tatttttcat taaaaatgct taagtacgca tttcaatgca ccacgatagt gcaacaaacc
    38461 tggcgtgcac catgatgatc attccatcag gtacagcttc ccagcgacgt atcagtcacc
    38521 gtagacatta aagtcgaaat actttttcgc catcttgtcg taggtgccgt cctgacgcag
    38581 ctcgccaagc gccttattga aggcagccgt cagttcagca taatctttac gtagccctac
    38641 accggtgcca tcaccgaagt attttttgtc ttttactgat gagccagcaa aagcgaaatc
    38701 tttaccagca ggttgcttga ggaatccttc gctggcagca acttcatctt gtaacgcagc
    38761 atccagacgt cctgcagcca gatcggaata gaccaaatcc tggttggcat aggccaccac
    38821 atccacgcct ttactacgcc aggtctcgtt agcgtaagct tcctgggttg atccctgcag
    38881 cacaccaaca tgtttacctt tcagtgaatc cagcgttggc tgaatcggtg aacctttggc
    38941 agcaatcaaa cgagaatctg cggcgtacag cttgtcggag aaggcaatct cctgctgacg
    39001 tttatcggta atggaaagcg acgaaataat agcgtcgatt tttttcgctt tcagtgaggg
    39061 gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc acctgcatcc gtttgcacat
    39121 ctcgttaccg agatcgatat caaagccaac aaaatcacct ttagcatctt tcgacgagaa
    39181 cagcgcatag gtggtatcgg ttccgatacg taccgtctcc ggtagcgccg catagctgga
    39241 agccgctgcg gagagaccga ctaataaaga cagagcgaga atcgacttct tcatacataa
    39301 ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt
    39361 catgcaggtc ttatgccatc ttgacgacaa cagtaacatt caacgttaaa tatgttaata
    39421 agacattgca ttattgtcct gaagttgaag atagcaggta tagcggttga atcgcagcgt
    39481 ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc gcggttgcaa aattgccctg
    39541 aaacagggca acagcggagt tatgcgccct gccagcgggc aaagagatct tcaggaaggg
    39601 ttattgcaaa ctggtcaaga acacgattaa ccgtctgatt tatcacatca tcaagggatt
    39661 gcgggcgatg ataaaacgcc ggaacgggag gcataatcac cgcaccgatt tctgccgcct
    39721 gagtcattaa acgcagatgg cctaagtgca atggtgtttc acgcacgcag agcaccaacg
    39781 ggcgacgctc tttcagcacc acatctgccg cacgggtcag taagccatca gtatagctat
    39841 ggacaatgcc ggacagggtt ttgattgaac agggtaaaat caccatcccc agcgtctgga
    39901 aagaaccgga agagatgctg gcggaaatat cgcgcgcatc gagcgtgaca tcagctaatg
    39961 cctgcacttc gcgcagggaa aaatccgttt cgagggataa ggtctggcgc gccgcctggc
    40021 tcatcaccag atgcgtttcg atatctgtga catcgcgcag aacctgtaat aagcgcacgc
    40081 cataaatcgc gccgctggca ccgctgatgc ctacaatgag tcgtttcata aatagttgcc
    40141 ctgtcagact tgcaggcaga ctttgcagta tttcggcggg agttgcaagt cagggtgcca
    40201 gaccggcacc ctcagcgaag gcatcatcct tcgttatgca tttcgagatt ttccacttcg
    40261 ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg tatcgaggaa atcgaggtag
    40321 ccctgatcaa catctttggt gacgtagacg ccgttgaaca ccgagcattc aaactgctgg
    40381 atatccggat tttcagcgcg aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac
    40441 ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc gaccgtgagc gatcagttcc
    40501 gtggcgctcg gcatatcaat accataaacg ttcgggaagc gaatttccgg tgccgcagaa
    40561 gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct cgataatctg ctcagaagtg
    40621 gtgccacgga cgatggagtc gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg
    40681 ttggcattca gtttacggcg cacggactta cgacgcagct gctggcccgg catgataaag
    40741 gtgcggccaa catagcggtt tttaacgaag ccctggcggt acggtttgcc cagaatacga
    40801 gcaatttcca gcgcgatatc acacgaggtt tctgggatcg ggatcaccac gtcgatatcc
    40861 agatcttccc attcgcgggc aattttctcg cccagtttcg tgcccatatt cacacgcgcg
    40921 ctgtaaacgg aaattttgtc gataaacgag tccgggcggg caaagtatac atactcaaac
    40981 aggcacggat tgctgaccgg attgtcagca cattgacggg taaacaactg cccttcttca
    41041 gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga aatcaaagcc cagcgtatcg
    41101 agcgctacgc tttcggaagc gaccatatat tctgtacggt tctcgtcaat atcacgtttt
    41161 cccagtacca gcggacgaat cccgtttgga tcgcggaaag caaccatacc gtggccgata
    41221 atcatcgcca cacaggcata cgcgccgcgg attaagcggt ttgtggcagc aatggcagcg
    41281 aaaatattgt cggcttccag cgggtagtgg cggaagttgt ccagctcgct ggcgaagata
    41341 ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt
    41401 tttttacgca actcgtgagc gttggtcaga ttgccgttgt gggcaagcgt aatgccatac
    41461 ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg agctgccagc cgtagggtaa
    41521 cgcacatgac caatgcccat attgccctgc aaacgctgca tatggcgagc ttcaaataca
    41581 tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt tattggcatc tatggtgatg
    41641 atgccggcgg catcctgacc gcgatgctga agcaccgtta aggcatcata aatcgactgg
    41701 ttaaccggca taacaccggc gataccgaca ataccgcaca tacgtctttt tcctcgttaa
    41761 gccacatctc agagcactta cgctctgggc aagaaacttg acgagctttg cagataatca
    41821 aaaaaccatc tgatgataaa actgaattgc gggatcagct gtgatttgct ccagtcttca
    41881 cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa tggcggcaac aatcaacaca
    41941 ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg tgcctgacaa ccccgttttc
    42001 tccaccaact ggcctatcac gaagttcacg atagcaccaa cgatcagggt agcgataaac
    42061 agtaccgcga tggcaatccc atttcgaacc agttcgtctt caaagcccgt aaaccagact
    42121 gacaggtaag tgtagtaatg actggcaaca aagaaagcac aaccccatgt caccagcgat
    42181 aacgcttcac gaacaaagcc gtggatcagg ctaaccagag aggaaaaagc aatcaccgcg
    42241 attatggcgt aatcaatcca gaccatatgt gtcccacgat tttacgccct gtcatcctgt
    42301 tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc
    42361 aataaaaatg gcgctgaaaa aatattcaac gccatcgact ttttatgcct ttgcggcatc
    42421 gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga cctacggttc taacactgcc
    42481 caagcctgat aagacgcgcc agcgtcgcat caggcaagac tgtattaatt cggcgtatag
    42541 cccattacca cgccacttaa gccagaaagt tgcttcaact cacccagcga acctttcagc
    42601 ttatctttcg aggcatccgg cccaaccaga atacgggtaa ttttaccctg cactggcgtg
    42661 gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat ctcattcact
    42721 ttatcggcat ttttcagcgc acccagttgc acaacatagg ctttacccgt tggcgctgct
    42781 ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtacttc aacctttggt
    42841 ttaggcggct ccaccggttt cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac
    42901 tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc atcacctgcc
    42961 cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca acgcctgggt ggcggctggc
    43021 atcatatcag gctcatcacg atcgcccgct ttcggcacca gcgggatagc cgcgaactca
    43081 tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac ccccagcgcc
    43141 accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc ttctcctcgc
    43201 gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa ccacacacca gcacggtgtc
    43261 ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gcaacgctat caaatgattt
    43321 gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg
    43381 cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg caatatcttt
    43441 atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg
    43501 cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga
    43561 ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc
    43621 attttcactg acttccagcc cgctggcacg cagtgccgcc agcgccgttg cggcattcgg
    43681 ttgcgggaca agcggcaacg gcagattttc cagcgtgccg tgagcatcgc taaacgccca
    43741 gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtaaca gtgcaccttt
    43801 ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa ttgccggttt
    43861 ttcgctgcgg aagatgcctg ctttctcgag accaatactt tcgcgatctg gacccagcca
    43921 gtcggtatga tccagcgcaa tactggttac taccgcgaca tcggcatcga caatattggt
    43981 tgcgtccaga cgaccgccca gccctacttc cagaatcacc acgtcaagtt gtgcctgctt
    44041 gaacagccac aacgccgaca gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg
    44101 tgccgattca atctccgcaa aagaggcggt gtgggccgat tccggcaatt cctggccctg
    44161 cacacgtacg cgctcggtat aacgcaccag atgaggcgaa ctgtagacgc ccactttgta
    44221 ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc cattcgtacc
    44281 cgcaacggta aacacaaatg gcgctggttt caggacgcca agacgcgccg cgacctggct
    44341 cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat aagaaagcca
    44401 cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat ccgctgattc
    44461 gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca gttatcaggc
    44521 ctcaggttcc tgatccggta ccgggggtac cactacgcct tcacgcggcg cttcaggatt
    44581 cggcgctggc agattcatca acttcgccag aatgctcgcc agtttcaggc gcatttccgg
    44641 acgacggacg atcatgtcga tcgcgccttt ctcgatcagg aattcactgc gctggaatct
    44701 aggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccgattaa
    44761 cgctttaggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc
    44821 catcgtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
    44881 tgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
    44941 accagaggcg gagaagcaga tcagcgggca gttatcttcc tgcgcctgct caacggcacg
    45001 cacgaaacgt gcacccacaa cagaccccat tgaaccgccc ataaaggcga actcgaatgc
    45061 cgcagcgaca accggcattc catacagagt gcctttcatc accaccagcg catctttttc
    45121 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
    45181 cagctcatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt catctaacag
    45241 gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg gacagacctc
    45301 aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac acttagtcca
    45361 caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat
    45421 ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta tctttgggga
    45481 cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa acataacagc
    45541 ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt
    45601 gccgcagcgc gtttgtgacg gattatttcg atgacgcccg gcaaaataga aaccacaata
    45661 atcccgacga tcagcagctt aaggttatcc tgaaccatcg ggattgtacc gaagaaatag
    45721 cctgcgtagg taaaaagcag tacccacaac agtgcgccga tcacgttata agcagcgaaa
    45781 tgacggtacg acatgtggcc cattcccgca acaaacgggg cgaacgttct gacgatcggc
    45841 acaaaacggg cgagaataat cgttttgccg ccatgtttct cataaaactg atgggttttg
    45901 tcgagataac tgcgacggaa aattttcgaa ttgggattac tgaataactt ttcaccgaac
    45961 aaccgcccga tagtgtaatt gaccgcgtca cccacaatcg cggcgatcag catcagcact
    46021 accatcatat ggacgttgag atcgttggtt tccagtgatg ccaacgctcc ggcaacaaac
    46081 agcaatgaat cacccggtaa aaacggcatt accaccagac cggtttcaca gaacaaaatt
    46141 aagaacaaaa tggcataaac ccagacgccg tactccgcga ctagctctgc cagatgcacg
    46201 tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatatttgc ctatgccttg
    46261 tactcgtcat ctttcaggct gtaactacgt tggctacagc ctgaaatgtt ccgagcatta
    46321 ttctcgttag tccgccagaa atagcgggcc cattggcggt tttggaagat cataccggtc
    46381 agggtaatcc accgcgacca gatacagccc ttccgctttt gccgttgctg ccgccagcgt
    46441 tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt gggcaccgac
    46501 ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaggcat tcgctttgat
    46561 atcaaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt tgcgccacgg
    46621 ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca
    46681 ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg ttaccccttt
    46741 actcagtacc gccgggcgca gccgatgatt gtagatgatg tagcgataac ggcgagccgt
    46801 ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca cggcgatgtc
    46861 accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg tctttgcgct gcgcggttgt
    46921 ttcgaaatgc accacttgcc cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa
    46981 gacggtgatg ggttcgttcg ccacctggga gagcgccttt tccagcttct cctgcacact
    47041 gcggacttca ttctgccgtt gccagccgta atacttactg ccgtcgtact caatgcccag
    47101 cgcaatttta taaactggca gttgttgctg gtcggacatt agtacagata ctcctgcacc
    47161 agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt atcggccacc
    47221 gaccagaact ggacttgctc cggcataccg tagtcattac gcacgcagcc aacagaaaga
    47281 tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt cgttctcttc agagagcaca
    47341 atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg ccgccagcgg acgcagtgct
    47401 tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac gcttgccgaa
    47461 atcatcagcc cttcgtcctg caggattttg cgtacttcgt caacgatacg acgttcttca
    47521 cgcacgctac cttcgctatc cggcaacaac ggcagcatat tgaacgccag ttgacgaccg
    47581 aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt tcgcactctg ccccgctaac
    47641 gcatcgaccg cttttttgcc ctgggcggag gctgaaatca ggctggtaac actgatacgc
    47701 gacaaaccgc cctgatcgat taacggtttc agtgccgcca gcagctggct ggtcagactg
    47761 tctggtacgg cgatgacatt ccggttgcgg taatcggtca gtacaaacgg gtttacttcc
    47821 ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc cgctgctgtc gatcaccagg
    47881 caacctgagt tggtcgcttc ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa
    47941 aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat cctgcacggt gattgtttta
    48001 ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg ccagtgcata aatttcccca
    48061 accgggaact gacgttcagc cagcgtttca agcagggctt cgcccatagc gccagttgcg
    48121 cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa tagcaaagct
    48181 ccctgccaat gttacagcag agagcatgaa gaagagatta acgtgccgga tgatgaacgg
    48241 cgttaaaacc cagtttacac agcaatgatg ccgcactggc gtcatcacaa attacataca
    48301 gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac tcacccggta
    48361 tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca
    48421 gccttttcag cgtcggttga tcgagcggac catgcagcgt aatgcggcca aactcaggcg
    48481 cgggcagcaa cgtatccagc gcaacgtgtt gttcatgccc aataaacttg ctataggctt
    48541 caaacacttg cgtcgtgcca cgcgctttac cctccagggt atagcctgcg atatgcggcg
    48601 tgccgatatc cacttttgtc agcaattcca cattaagttc cggctcgcct tcccagacat
    48661 ccagcaccac gcttaacttc tggccttcat tcaggcaggt cagcagcgcg gtattatcga
    48721 cgactgcgcc acggcaggcg ttaatcagaa tcgctccggg cttcaggcta cggatcagtt
    48781 tttcatccgc cagatgtagc gttttgtacg gaccatcttt aaagagtggc gtatggaaag
    48841 tcagaatatc cgcgcgctgg actaactcat ccagcgagcg gaaatcaccc tcatccccac
    48901 ggtcggcgcg aggcggatcg caaagtaagg ttttaatccc taacgcttcc agtcgcgcct
    48961 gcaatcgacg tccaacgtta ccaacgccca cgatccccac cgtacggtcg tgcagtgaaa
    49021 atccatcgcg ttcggcaagc atcagcaggg aggagaaaac atattccacc accgcaatcg
    49081 cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga
    49141 catggtcggt ccccgcagtg gcagttccaa caaatttaat gggttttcct gccagcaaag
    49201 attcattcac tttcgtgacc gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga
    49261 cggggattgg acgcccggga accgcgatca cctcacccaa acggctaaat aagtcgcggg
    49321 cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta tgagacgaga
    49381 gttaaccgga caagtatgcc ataatctcgc ggccaggcat acttgcgaag atttcaggta
    49441 taaggatacg taatgataca acctatttcc ggccctcctc ctgggcagcc accaggtcag
    49501 ggagataacc tgccgtctgg cgcgggcaat cagcctttat ccagtcagca acgtacttcg
    49561 ctggaaagct taatgacgaa agtgacctca ctgacgcaac aacaaagagc agaactgtgg
    49621 gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc gcgtcacttc
    49681 cctgccgctg agcataatct ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc
    49741 cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaatcatcg tcaggcggtc
    49801 acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc accggagcaa
    49861 ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg ttattccgca accacagcag
    49921 cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca atgcgctaaa acagctggtg
    49981 accaaacttg cggcggcaac gggggaaccc agcaaacaga tctggcaatc gatgctggaa
    50041 ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac tgtttaacca tctggtgacc
    50101 tggctacagg cgcgtcagac gctaagccag caaaatacgc cgacgctgga atcactacag
    50161 atggcgctaa aacaaccttt agatgccagt gaactggcgg cgttatcggc atatatccag
    50221 caaaaatatg gcctttctgc gcaatcatcg ctttcttctg cccaggccga ggatattctt
    50281 aatcagcttt atcaacggcg ggttaaaggg attgatccgc gtgatatgca accgctgctt
    50341 aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg
    50401 atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc ccgacgaaat
    50461 gacagtatcg tgacaataat acccagcacc gcagagatcg ccccggcaag aaataccgaa
    50521 gagtaaccaa acgtggtcgc cagcattccc gccagcggcc cggagacgcc gagggcgata
    50581 tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa cttgtgaggg gacgcgttta
    50641 accacctcca cgcccagcgc aggaaagata agcgaacatc cggctccggt taacgccgcg
    50701 cccgctaatg cgacccatgc acctggggct tgccagagca gcaacaagcc caccgtttct
    50761 acaagcagag agacaatcgc cactttcacg ccgccaaaac ggtccggcat ccagccaaac
    50821 atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc
    50881 catcctttgc tggcaaagta gagcgaaacg aaagtcccga taaccgcaaa accaacgcct
    50941 tgtagtgcca gccctaaccc tggtttccag ataagcccga caacgctcca cagcgatgga
    51001 cgttctcccg ccagggccgg tactttgcgc actgtgccgt tacaggccca cgccagtaag
    51061 ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt aatggctatg aatcaacagg
    51121 ccaagcggcg caccaacagc gagggcaccg taaatcgcca ttccgttcca tgacatcact
    51181 ttgccagagt gttttggccc tacgatgcct aacccccagg tcagagcgcc tgtcagtaac
    51241 tggctttcac caaacccaag aatcaaacgc ccgatgacca acagggcaaa tttgaacggt
    51301 gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc cagccagacc acaagctaac
    51361 atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg ccagtcgccc ggcgtaacca
    51421 cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa tgccgaccat ggtattgcca
    51481 tagcccagat catgatgaac aaacagcggg ataaccggca acggcaaccc tacggtcatg
    51541 taggtgagaa aaaccgcaaa agagatgcgg aagagcgaaa aactggcaga agatcgtgtt
    51601 tcggtttggc ttacagcagt catgcattac tccagaatgc agcgcaaggc gaggagtatc
    51661 cccgtctcat ctctctggtt tcagggttac ggtgcgttgg caggatttaa cgcgtacgtc
    51721 ttttcagaag gaaatcgaca aagcgggaag tttgcctaga actggtggcg attgtcaatg
    51781 atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt gccggaggcc gcattggcgc
    51841 gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta ctccggccta ctgcgaatta
    51901 atcttttcag cttgcgcatt accagcgtgg cgttggtgcc gccgaagccg aagctgttag
    51961 acataacagt ggtcagttcg cgatcggtcg tttcggtcac gatgttcaga cccgcagcct
    52021 gctcgtccag ctcttcaatg ttgatgctcg gggcgataaa gccgtgttcc agcatcagca
    52081 gagagtagat agcttcctgc acacccgcag cgcccagtga gtgaccggtc atggctttgg
    52141 ttgcagaaat cgccgggctc ttatcgccga acacttcacg gatagctgcc agctctttca
    52201 cgtcgccaac cggagtcgaa gtaccgtgag agttcaggta atcgattggg gtatcaacgc
    52261 catgcatcgc catctgcata caacgtactg cgccttcgcc agacggagca accatgtctg
    52321 caccatcaga ggttgcgccg tagccaacga tttcagcata gatgtaagca ccacgcgcca
    52381 gcgcgtgttc cagctcttca accactacca taccgccgcc gccagcgata acgaaaccgt
    52441 cacggtgagc gtcgtaagta cgggaggctt tttccggggt gtcgttgtat ttagtagata
    52501 gcgcacccat cgcgtcgaat tcgcaggcca tttcccagca cagctcttcg ccgccgccag
    52561 caaacacgat gtcctgtttg cccagttgga tctgctctac tgcgttaccg atacagtgtg
    52621 cggaagtcgc acacgcggag ctgatggagt agttaacgcc atgaatttta aacggcgtgg
    52681 cgagacaggc agaaacgccg gatgccatcg ctttagtgac cacatacggg ccaaccgctt
    52741 tcaggccgcg cgggccgcgc attgcgtcag cgccgaacac ctggaaacgc ggggaaccgc
    52801 cgccggaacc tgcaatcagg ccaacgcgcg ggttattctg gtaagcttcc ggagagaggc
    52861 ccgcatcagc gattgcctgc tccatagaaa ggaatgcata aatggatgcg tcgctcataa
    52921 agcgcacaac tttgcggtca atgaggccag tggtatccag ttttatgttg ccccagacgt
    52981 ggctacgcat gccggaatcc ttcagctcct gagagaaagt gatccctgaa cgtccttcac
    53041 gcagagatgc caggacttcc tgctggttat taccgatgct ggaaacaatg cccaggccag
    53101 taatcactgc acgtttcatt caatacctct gtaagtcgca catagagtaa gtttcgaatg
    53161 cacaatagcg tacacttgta cgccgaacaa gtccgatcag ccatttaata gagatatttg
    53221 cgcagcctta cacacatcgc taagatcgag ccaccgcctg taagacgagt aacttacgtg
    53281 aaacactact ccatacaacc tgccaacctc gaatttaatg ctgagggtac acctgtttcc
    53341 cgagattttg acgatgtcta tttttccaac gataacgggc tggaagagac gcgttatgtt
    53401 tttctgggag gtaaccaatt agaggtacgc tttcctgagc atccacatcc tctgtttgtg
    53461 atagcagaga gcggcttcgg caccggatta aacttcctga cgctatggaa ggcatttgat
    53521 cagtttcgcg aagcgcatcc gcaagcgcaa ttacaacgct tacatttcat tagttttgag
    53581 aaatttcccc tcacccgtgc ggatttagcc ttagcgcatc aacactggcc ggaactggct
    53641 ccgtgggcag aacaacttca ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta
    53701 ttgctcgatg aaagccgcgt gacgctggat ttatggtttg gcgatattaa cgaactgacc
    53761 agccaactgg acgattcgct aaatcaaaaa gtagatgcct ggtttctgga cggctttgcg
    53821 ccagctaaaa acccggatat gtggacgcaa aatctgttta acgccatggc aaggttggcg
    53881 cgtccaggcg gcacgctggc gacatttacg tctgccggtt ttgtccgccg cggtttgcag
    53941 gaagccggat tcacgatgca aaaacgtaag ggctttgggc gcaaacggga aatgctttgt
    54001 ggggtgatgg aacagacatt accactcccc tgctccacgc cgtggtttaa tcgtacgggc
    54061 agcagcaaac gggaagtagc gattatcggc ggtggtattg ccagcgcgtt gttgtcgctg
    54121 gcgttattac ggcgcggctg gcaggtagcg ctttattgcg cggatgaagc ccccgcgctg
    54181 ggtgcttccg gcaatcgcca gggggcgctg tatccgttat taagcaaaca cgatgaggcg
    54241 ctaaaccgct ttttctctaa tgcgtttact tttgctcgtc ggttttacga ccaattaccc
    54301 gttaaatttg atcatgactg gtgcggcgtc acgcagttag gctgggatga gaaaagccag
    54361 cataaaatcg cacagatgtt gtcaatggat ttacccgcag aactggctgt agccgttgag
    54421 gcaaatgcgg ttgaacaaat tacgggcgtt gcgacaaatt gcagcggcat tacttatccg
    54481 caaggtggtt ggctgtgccc agcagaactg acccgtaatg tgctggaact ggcgcaacag
    54541 cagggtttgc agatttatta tcaatatcag ttacagaatt tatcccgtaa ggatgactgt
    54601 tggttgttga attttgcagg agatcagcaa gcaacacaca gcgtagtggt actggcgaac
    54661 gggcatcaaa tcagccgttt cagccaaacg tcgactctcc cggtgtattc ggttgccggg
    54721 caggtcagcc atattccgac aacgccggaa ctggcagagc tgaagcaggt gctgtgctat
    54781 gacggttatc tcacgccaca aaatccggcg aatcaacatc attgtattgg tgccagttat
    54841 catcgcggca gcgaagatac ggcgtacagt gaggacgatc agcagcagaa tcgccagcgg
    54901 ttgattgatt gtttcccgca ggcacagtgg gcaaaagagg ttgatgtcag tgataaagag
    54961 gcgcgctgcg gtgtgcgttg tgccacccgc gatcatctgc caatggtagg caatgttccc
    55021 gattatgagg caacactcgt ggaatatgcg tcgttggcgg agcaaaaaga tgaggcggta
    55081 agcgcgccgg tttttgacga tctctttatg tttgcggctt taggttctcg cggtttgtgt
    55141 tctgccccgc tgtgtgccga gattctggcg gcgcagatga gcgacgaacc gattccgatg
    55201 gatgccagta cgctggcggc gttaaacccg aatcggttat gggtgcggaa attgttgaag
    55261 ggtaaagcgg ttaaggcggg gtaatctgct ctcgcagtgt ttgccggatg cggcgtgaac
    55321 gccttatcca acctacgtgt gacgtgtagg cctgataaga cgcgataagc gtcgcatcag
    55381 gcatggtgct ccagacgccg caacttacag ctgtgacgcc tgctgaaaca ggttttccca
    55441 catatcggtc accagcgcct ggtcacgcgg cgacaattca ccggcaccaa tggctttttc
    55501 cagactctgg ctaacggtcg tatgcaccgc ctgagcggag tggtcatcac cactttccag
    55561 ttctgcgatg gctaatgtca ggtggccacg caaataccca ctggcaaaca actcatcatc
    55621 actggcatgg tcaaccatgc cgtcgattaa tgccagaatg cgtgattcaa actccgcgat
    55681 catcttcttt cctcattgta aaacgtggcg ctggcttcag ttgagcgctt ccggccacgg
    55741 gaactgttcc gccgtaagtt ccggcgtgtg ataataattt tgtagtgctt taatgaagcg
    55801 tgccgggcgt tccggaatgc catttgtcag ataatccatc acctgcgcat gtacccgccg
    55861 ttgaaacacc acacgatccg gttcgaaatc cccttccaga ttgtcgcaac tgacgttaaa
    55921 cggatatcca gccgccacgc agaataacca gtcgaacgcc tgcggcttca cttcaacatc
    55981 ttcaaactgg ctttgcgtct gggcatcgcg tccgtccggg caataccagt agccgaaatc
    56041 aaccagctca cggcgcgctt ttccggcaat acaccagtgc gaaatctcat gaattgcgct
    56101 ggcataaaag ccatgagcaa agacgattcg gttatacggt acttccgcat cagcaggaag
    56161 atagatcggt tcgtcgtcgc ctttaatcag acgggtatta aattcatcgg caaagcagct
    56221 attaaaaatt tcaatcaact gctcgtagtg gtgtgtactg ttcattagtt catccccaac
    56281 cagtggagga tctcctgtcc gtggctatcg taaagtaatt tggcactcat caccgccgag
    56341 acgataacaa tcatcgggcg gatcagcttt tgtcctttgc tcaacaccaa ccgtgaaccc
    56401 atgcgcgcgc cgaggaactg ccccaccagc atgacaaagc ccgtcgccca aatcactttg
    56461 ccgccgagaa taaacagcag cagaccgcca atgtttgacg ttgcgttgag taatttggcg
    56521 tgagccgtgg ctttggcgag gttgaatccg cacagcataa cgaaggccag cgcgtaaaac
    56581 gatccggcag ccgggccaaa gaatccgtca taaaaaccga cgcagccacc ggcaattaac
    56641 gcgaagggta aaccgtacat ccggcgctgg cgatcttctt cacccagctt tggcatcagc
    56701 aagaaataga ggccgataca aatcaccaga atgggcaaaa tctgccgtaa gacatcagcc
    56761 tgaacgtact gtaccaacag tgcgccgctc attgagccga caaaggtcat ggcaatattg
    56821 agtttctgat cgcttaaact aaccactttg cggcgaataa agtagatagt ggcggaaata
    56881 gagccgccgc aggcttgcag tttattggtt gccagcgcgt tagcgggaga cattcccgca
    56941 gccatcaatg ccggaatggt gagtaaccca ccgccaccgg caatcgagtc gataaatccc
    57001 gccagcatgg cgacaaaaaa gagaactccc agcaacagcg gagaaaccat aaacaggcta
    57061 ttaaacgttt ccattagatc acgtgctcat ccagtagcgc ctggcaggaa ggcggcaacg
    57121 gaggcggtgt cttcttctca ggctttgttg ttcccggttt tggaggttca aaccagcttt
    57181 gcagttctgc cccgcaacca tcgcctgatg gcggtaaagg ttgatcttca cactccagac
    57241 tatcggcagg gcaacgtaat cgtacatgca tatgcgcgcg atgctggaac cagggtcgca
    57301 ctttgcgcaa ccagtcgcga tcggtgcccg catcaaggca aagttgttgt ttaatcgccg
    57361 gattaacaaa aatgcgcgtg acgtctttgt cctgggcggc gagtttgatc aagctgaaaa
    57421 tttctggctt ccacagcgtg gagacaacgt gtttaccgtc gcgggaaact aagtccagtg
    57481 cttgcgggcg caagagctgc gcggaggtcc agcgagtttt cggcagttgc agaaagatat
    57541 cgacatccag tccggtctgg tggctggcat gaccgccgtt gaaacgccca ccagcgggca
    57601 tccccatatc gccaatcagc accgtaccca tgcccagatt gctcacctgg ctactcagac
    57661 gctggataaa catcaccaga tccgggtgac cgaaatagcg acgctgatcg gtacgcatga
    57721 cctgataatg ttcggactgt atcggcaacg tgtcagcgcc gacaatacag ccattagaaa
    57781 aactgcctat cgattgtgcg ctacccggca caggttgggt tattttttgc cacggcgtcg
    57841 ctgccaggct agcactactg gcaagcagag ccagcagcgc aatcgcggtt ttgttcattt
    57901 tttaccagcg tggaatatca gtcttcacat cggcattttg cgcccgctgc cgtaacaggt
    57961 gatccattaa aacgatcgcc agcatcgctt cagcgatcgg cactgcacgg atcccgacac
    58021 agggatcgtg acggcctttg gtgatcatct caacttcttc gccaaagcgg ttaatagtgc
    58081 gccccggcac ggtaatgctg gaggtcggtt tcagcgccat atgagcaatg atttgctgcc
    58141 cgctgctgat accgccgaaa atgccgcccg catggttgct ctggaaaccg tctttggtga
    58201 tttcgtcgcg gttctggctg ccgcgcagcg ccactacgtc aaaaccgtcg ccaatttcca
    58261 cgcctttcac cgcgttgatg ctcatcagcg catgggcgat gtcggcatcc aggcggtcaa
    58321 agaccggctc gccaagtccg gcggggacac cactggcaac aacggtgact ttcgcgccga
    58381 tggagtcgcc ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg attttgtcgg
    58441 ggtccgggca gaagaacgga ttttgttcaa ccagcgacca gtctttgatt tccagcggaa
    58501 tgtcgcccat ctgggtcagg cagccgcgga tttcaatacc aaatttctcg gcgagatatt
    58561 ttttggcaat cgcccctgcc gccacgcgca tggcggtttc gcgggcggaa gaacgtccac
    58621 cgccgcgata atcgcgcaga ccgtattttt gttcgtaggt gtaatcagca tggcctggac
    58681 ggaagacgtc tttaattgcg ctgtaatcct gagaacgctg gtcggtattt tcgatcaaca
    58741 agccaatgct ggtgccagta gtaacgcctt caaaaacacc ggagagtatt ttgacctgat
    58801 ccggctcgcg gcgctgggtg gtatagcgcg atgtcccagg gcgacgacga tcaaggtcat
    58861 gttgcaggtc cgcttccgtc agcggaatgc ctggcggaac accatcgacg atgcagccga
    58921 gcgccagccc gtgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc
    58981 cagccatcac ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg
    59041 aaatgttctc gtgcggcaat aagctgctct ttagtgagca taaacacacc atcgccgccg
    59101 ttatcaaact ccagccaggt gaacggaaca tcgggatatt gttccataag atgtaccatg
    59161 ctgttgccca cttcacaaat caacacgcca tcatcagcaa ggtaatctgc cgcgttaccg
    59221 agaatgcgac gcgtcagttt caggccgtcg gtgccagatg ccaggcccag ttccggctcg
    59281 tgacggtatt cgtttggcag gtcggacata tcttccgcgt cgacatacgg cgggttagtg
    59341 acaatcagat cgtattgcac tttcggcaaa tcacggaaca gatcggaacg aatcggaatg
    59401 acgttgtgga tcagaccgtg ttcttcgatg ttctgttcgg caaccgccag cgcgtcggga
    59461 gagatatcta ccgcgtcgac ttccgcttcc gggaaggcat aagcacaggc aatggcgatg
    59521 cagccgctac cggtacacat atctaaaata tgctgcggtt gcttgctgat gagtccggca
    59581 aatttattgt tgatcagttc accaatcggc gagcgcggca ccagcacgcg ttcatcgacg
    59641 taaaattcat gaccgcagaa ccaggctttg ttggtcaggt aagccaccgg aatgcgctcg
    59701 ttgacgcggc ggatcacgcg ttcaacaata cggtgttttt cgctggaggt cagacgcgcg
    59761 gtgcgcatat cttccgggat atccagcggc aggtagagcg atggtaacac cagctgtacg
    59821 gcttcatccc acgggttatc ggtgccgtga ccgtaccaga tatttgccgc gctgaagcgg
    59881 cttaccgacc agcgcaatat gtcctgaatg gtttgcagct catttactgc ttcatcaacg
    59941 aaaattttat ccacgtattc ctccagggca tgatcgcaat aatttcggcg gctagtttgc
    60001 catgaagatg acgataaatc agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa
    60061 cgatcgattg tgctacgagt cgggtaaact gtaggaaaat tagaaataga gacagataaa
    60121 tgaaaaagaa aacaacactt agcgaggagg accaggctct gtttcgccag ttgatggcgg
    60181 ggactcgcaa gattaagcag gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg
    60241 aagtgccggt gaaacgcttg atccaggagc aggctgatgc cagccattat ttctccgatg
    60301 agtttcagcc gttattaaat accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc
    60361 attttgaggc gaagaaactg cgccgcggcg attattcacc ggaattgttt ttggatttac
    60421 acggtctgac gcagctgcag gccaagcagg aactgggcgc gttgattgcc gcctgtcgcc
    60481 gcgaacatgt gttttgtgcc tgcgtgatgc atgggcacgg gaagcatatt ttgaagcaac
    60541 aaacaccgct gtggctggca caacatccgc atgtgatggc atttcaccag gcaccgaaag
    60601 agtacggcgg tgatgcggct ttgttggtgt tgattgaagt ggaagagtgg ttgccgccgg
    60661 agttgccctg aatgctggca gcaaatgccg gagcagcgta aacgccttat ccggcctaca
    60721 aatatgtgat aattcaataa attgcaattc acttcgtagg cctgataaga cgcatcagag
    60781 caatactacg aacttcagtt cattcaatac atactgaaaa cgacacgtcg aatcaatcgg
    60841 ttgcccacta ctggtattca cattcaccac aatatcgtgt agtactttat ccgttaccgg
    60901 atgacgagtt ggcgtaaccg ttaccgttga tttatcacaa ccgtaagagg taaaggttaa
    60961 caattctgat accgcgcggc agtatgctcc tctgccatca ttatcagaaa aattactgcc
    61021 atcacagagt ctgccgcctt ccatcaggta ggtcgaaagc gtggtggtca ctgttgaccc
    61081 cgtggactga attcgtaacg ccttattatg gctgacataa ttttcactcc aggagatatc
    61141 gtatgaagcg accggtatcg ttgggcatat tgtgttatgc actgtagggg ccgtatagct
    61201 tccaaatccg ggtaaatctg tataggaata aaaggatgcg atccaagggc aaccataatc
    61261 atcagatgca acggtaattt taaacgtcat atatgcacaa cgaaaaattt tcgtatctat
    61321 gccagggcaa tttgagttat taattgtatc aaagtcagtc agtaccaaag ttggagctga
    61381 acccgaccca gaccaggaaa taacccgcaa cgtatcgcct ttatacgcaa aagaatcata
    61441 tttcccatat gcaacctcct ttggcgttat caccgtaaca ttgaaaacac tgctcgctac
    61501 agcaccggtt accttgtcca tggcataata aactctcaca ctgtcaaaac tcgcactcat
    61561 aagtttatct ccagcataac tcttagatgc gccgacaaca ctcagaacga gcagcattag
    61621 gctcgcgaaa attatggtcc attttttcat ttcagaaata ctcaaaggtt gccgtcactg
    61681 tggcactaaa gtctccggac gttacatctc gcccgctctt tgcctgtaac caggtattaa
    61741 aatttaggct gttatttccc tgattcagcg ggagcgtgac gcctgttgta ttgttgattg
    61801 gcaccgatgt gcctgccgca gtttcaatac caatcccagc gccggatgct gatgacgagg
    61861 agtcaaaggc cagaaagccg ggtagagcac tatcttctgt tcctgtcaac gttactttga
    61921 cattaaacaa tgtagaacta tgacaatctt tcaattgaaa caccaatggc actcgctctg
    61981 actgcccaag gttcattaaa tcactggcgg caatggcagg aaaatgtact tctgccaact
    62041 ggctaccatt aatcactggc gtacacgatt tactgattaa attgccggta aagtgcaaat
    62101 tatctcctgc tattgtggtt ccactgccca gcaacatcag gctactaata aaaaacaacg
    62161 tacgcttttt cttcattggt aatctacctg taacgtggcc caggcctcaa agtccgcctc
    62221 tggcagaatg acaccactct tttttactgg cacagccgtt aacaccggta aatcggtttc
    62281 attcaccaca agtggcgtat tgatcgtaaa actctgccct gcctgtttga gctgaatgcc
    62341 taaaccagtg atattgctgc ttactgcagc attatcaaaa tcgcttggtg tgccactcca
    62401 gctcaaggtc atttccatta atgggtcgcg aaccgccgta tcgcaggtaa tggaatacgg
    62461 cacggtttgc tcataattgc tgccattaat gtcatcaata agcacatcct gaaaagttat
    62521 ttcaatggtt tgagcgttgc taatcgtgca gttaggcggc tgcactaaat aaccgtgaaa
    62581 agagacgttt gagagtgcca tcgagcaaaa gccccacagc aaaataagcg agatccgttt
    62641 cattggtaat ccaccacaat tgtggcactg gcgttaaatt cagctgccgc taacgttgta
    62701 ccactctgtt tcaccggcac agcttccaga acgggaactc ccgaggaaaa attaaatggc
    62761 aaccatgagc cagaggttaa gtcgagtatt gtgtgatcac tacttttctg aacccgaata
    62821 ccaaacccag gaatacccgt gctgataact gtttcaccgt taatcacgac cgttgtcgcc
    62881 tgcatctgta gacgcaggtc atccattgcc agattgttac aaacaagcga gtagtcaatg
    62941 ggacgtttat aatcaaccct attgatctta ttaatgaata cattaccaaa ctcaactgcc
    63001 gcaccattta ccgtacaagg aggaggggca tcgacaagca cccgtaaatt catacttact
    63061 gacttactcg ctgcaactgc aaagccgcta ccaacacaac atatcagtaa cccagttacc
    63121 gcccagacag aacaagatcc tttcatactg gctctcccgg cctcagcctt tgcggctctg
    63181 ctcttcatcg acctagcaac tgttaccgtt gcaagtgaaa aagagcggca tccgagcgcc
    63241 atagtcgttg acataggtca gtaccggcac gctacccatc ttcacattca gcggtgcaga
    63301 ggttttcggc gacatcacta ttggcgagaa accggctgcc ggattgccat ttttctgtgt
    63361 gctggcatta ctgaaaatga cgtaatacga cgtcgggtta ttgacggtaa attcctgacc
    63421 gttgcgggtt aaagtaatct tatattgcta ggggtttttc atgctgacgt tctccagcgc
    63481 cttcggtcgc cagaacaact taatacgcgt ttgtagtgct atttgcagcg tgttggcttt
    63541 gtttgatttt ggcgggatct cacgcacgtt gaagtagaac aggctttcac gatcggcagg
    63601 caatttgttg atatccggca ttccctgcac ttttacctgc ccgttcatca ttgaatcgat
    63661 gcgttgcacc ggtggcagaa cagttaacgg tgaactgatc ttgttaccct tttcatcttc
    63721 gatccagctt tgggccagat aaggcagttt cggatcgtta ttgcgcaacg tcacgctgat
    63781 tgatttgtcg ctttcattaa atatcaaacg ggtacgatct ggcgttacag aagcctgggc
    63841 agacaaactg gcagcgctaa gcagtaacgc tagcgtcgta gcacccaatt tggcagagca
    63901 aagtaaatca gacatggatc attccttaat cacgttattg gttagctgaa acagaaacag
    63961 ggggattggt gggtgtcacc tgttgcgtct gctcctgaat gacaggcttt atgtcatcag
    64021 gaacaaccgg cgcgacgttg cctttatgct ggcatggcag taacaagccg ttaaacagat
    64081 cggcaggcag cgggtccggc aggttgatat cgcaatgcgc aacaccactc cagaacacac
    64141 tcatgtgttc gccaggtttc actccagcca gataaacatt gccgtcatca tcgacaaggc
    64201 ccactgtctg ctcgttatca ttttttactt ctgcgccaaa cggaggatgg ctgccgtctt
    64261 gtaaacgcag caccgccatc gctttttgac cactgatgac ggcaaatttg cggtagccaa
    64321 tcgccccttc agttagcgtg gcttgtacca ccgactgggt tgcttcggcg ttttccggca
    64381 gtttgttgag gtcgatatac gcctgattgc ggtaatagtt attgacgtca gaaacgacgg
    64441 ctttaccaaa catattggta taaacagcag caccgttacc ttctaccgga acatcggcaa
    64501 cgccatcggc atcaatcaac aagcgtgtcc cgcccatatt ctgggtacgg tgaagtgcgc
    64561 caccgtgggc agtaagcgtt gcgccgccct gtaacgacaa gcccgcagag gtgtattgcc
    64621 cttcatggta gttcgcactg aggtcaacct gcgccagcga accatcatgg ctgtaatagc
    64681 cgtcaacgct ggtgtgtttg tcactggtgc cgacgttcaa ctgatagtga gtcgcgtcat
    64741 cgacacggct gaaataaccg acctgactgc tgtccgtccc actgccatag taccgttata
    64801 gctaacggta ctgttgtcgc cccacggcat actgagcgaa atgtacatgc ctttgtcggc
    64861 ctggttgtca tactcgtaac ggtagccagt catcgagatg ctgacattac gaatactgcc
    64921 catattgaaa tagtgcgaga gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta
    64981 ggtatggcgg gtatagttga gataaaccga aacacccgca tcgcggaagt tctggttata
    65041 agtggcggtg tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc
    65101 cagatactcg ctcatggtca taaagttctc ttccgagaag cgatatccag caaaagtaac
    65161 gcggctgttg agctggtcaa agtctttgga ataactcaca cggaaggagt taccgtccag
    65221 cgaacctttg ccataagcgg tgtctttatc cagtttggtg tgcgagtgag taacatcaaa
    65281 cgcgaccgcg ccgaatgtag acaaatcgcg accgacacca agcgccgcag actgatagtt
    65341 ttcatctccc agtgcgccgc catataacga ccagccgtta gcgatccccc aggaagcttc
    65401 agcaccagaa aaaaattcac cctcgacatg gtgtccccac tcttgcggac ggcccatcat
    65461 gatcttatag cgaacctgac ctggacgagt gaggtatggc atcgaggcgg tgctgatgtc
    65521 atattcctgc acctggccgt tctgttcttc aatgcgaata tgcaacgtac cggagacaga
    65581 atcaccaaga tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg
    65641 ccccatctgg ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc
    65701 gtagccgcgc agattgggag gcaacatttg atcgtcagta ctgacactgc caccaacata
    65761 gttaaaacca tcaaaaatat cggatctgag gtaatcctcg cccagcgcca gtttggcttt
    65821 taatgatggt aacgcccgcc tggcatagta gcgactccac tcccattttt ttgagtttca
    65881 tcgccgctga attcatcgtc atcattactg cgagtatgtt gatagttagt ctgccagtca
    65941 gcacgcatac gccacggccc taggttaacc ccaaccgtcc cgttgccgct gatctcgtta
    66001 ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt tgatgctgta gtccgcgacg
    66061 atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc tgtcgggata agtatattcg
    66121 aggtaagcct gtggcagtga aatgactaat gcggactggc ttaaatcagc cttaatttcc
    66181 atgccttcca gttgaccgga tttcaggcat ttagcatcgt ggctccattg cagatttttc
    66241 gccacgtctt cttttaaacc aaactgcgct accagttccg gtgtcagaca agcatagctt
    66301 ttgctcgcgt catcttcacc agcataccag taaatatcgt actcttccgc caatggctgt
    66361 ttatttagtt gaacctgtaa attgtatttt ccgggctcaa catatccctg gctggaaaaa
    66421 cgcttcatat caatttttgt gtcgcctttt aattccagaa aacgggaatc aaactgaatg
    66481 tcgtcttcag cccagacact gctataactc cctgacattg ccagcgcaat gcaccacgga
    66541 agaatccgta atcgaaaaag agaatggtca ggcatacacg tttctatccg tttttattct
    66601 tgttcggtca aaatacattt aaatccccca tagcagtgtc gctatggggg tatgccttac
    66661 aggtaagtaa tctggaaggt ggtatttgct tcaaaattac ccagatctgg cgcatcagct
    66721 gcgccgacca gccaggcgtt aaagttcagt gtctgcttcg ctttgccttt gttggtagaa
    66781 gtgtctttca ctattttctg gtcaataccc atgccgcttt tgtaagaggt acccagagag
    66841 tcaccgatcg ccaggctaac attgttaaat gccgcaccgg tatcggtatt gaaaatggtg
    66901 taataattgc cgctatttgc agaagaaacg gtaccagtaa aggtagtggt cattgtttcc
    66961 tgagtatcaa atacgcaatc ttgcagacga atctggaaac ttttcggcgt ggtggtgccg
    67021 ttatttttca gtttttcggc accgatatca cccatatcaa cttccagttt gtgatcgtcc
    67081 ggaacgatag aacacactga gtcttcaatg gtgccgtaaa aacgtgcagt accgttgtta
    67141 cccgcagcga ttgttgccgt agaggtgaac acgcccatca ccattgccgc agcaatagct
    67201 gttttaacaa acttactcat tatttgtcct taaactataa ataattaaaa atataaacat
    67261 tgcaatacat tgattcagtc aatagccaat gtttataccc agccattgaa tacggcatgc
    67321 gacccaaaaa gaacaggtcg tagaaaattg tattcattgg tgtggggccg catagtgatg
    67381 tttccacacc cctgatccaa tttaatttta ctaaagagta aatgtagatt taattaatat
    67441 attgacagaa gaaaggttat cttaaggaca
//
LOCUS       DAJVPT010000006        66534 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVPT010000006 DAJVPT010000000
VERSION     DAJVPT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OQM47_000390"
     CDS             complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OQM47_000390"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1478674.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OQM47_000391"
     CDS             complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OQM47_000391"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1478675.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OQM47_000392"
     CDS             complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OQM47_000392"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1478676.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OQM47_000393"
     CDS             complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OQM47_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1478677.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OQM47_000394"
     CDS             complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OQM47_000394"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1478678.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OQM47_000395"
     CDS             complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OQM47_000395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1478679.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10958..11551
                     /gene="yhgN"
                     /locus_tag="OQM47_000396"
     CDS             10958..11551
                     /gene="yhgN"
                     /locus_tag="OQM47_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1478680.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OQM47_000397"
     CDS             complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OQM47_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1478681.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OQM47_000398"
     CDS             complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OQM47_000398"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1478682.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OQM47_000399"
     CDS             complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OQM47_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1478683.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OQM47_000400"
     CDS             complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OQM47_000400"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1478684.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15655..16692)
                     /locus_tag="OQM47_000401"
     CDS             complement(15655..16692)
                     /locus_tag="OQM47_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1478685.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17025..17513
                     /gene="yhhY"
                     /locus_tag="OQM47_000402"
     CDS             17025..17513
                     /gene="yhhY"
                     /locus_tag="OQM47_000402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1478686.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17572..19302)
                     /gene="ggt"
                     /locus_tag="OQM47_000403"
     CDS             complement(17572..19302)
                     /gene="ggt"
                     /locus_tag="OQM47_000403"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1478687.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSRIITTVLQMVVNSIDYGMNVAEATNAP
                     RFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDP
                     RSVDDLTAGY"
     gene            19422..19862
                     /locus_tag="OQM47_000404"
     CDS             19422..19862
                     /locus_tag="OQM47_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1478688.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19849..20592)
                     /gene="ugpQ"
                     /locus_tag="OQM47_000405"
     CDS             complement(19849..20592)
                     /gene="ugpQ"
                     /locus_tag="OQM47_000405"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1478689.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20589..21579)
                     /locus_tag="OQM47_000406"
                     /pseudo
     CDS             complement(20589..21579)
                     /locus_tag="OQM47_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21581..22426)
                     /gene="ugpE"
                     /locus_tag="OQM47_000407"
     CDS             complement(21581..22426)
                     /gene="ugpE"
                     /locus_tag="OQM47_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1478690.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22423..23310)
                     /gene="ugpA"
                     /locus_tag="OQM47_000408"
     CDS             complement(22423..23310)
                     /gene="ugpA"
                     /locus_tag="OQM47_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1478691.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23408..24724)
                     /gene="ugpB"
                     /locus_tag="OQM47_000409"
     CDS             complement(23408..24724)
                     /gene="ugpB"
                     /locus_tag="OQM47_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1478692.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25121..25834)
                     /gene="livF"
                     /locus_tag="OQM47_000410"
     CDS             complement(25121..25834)
                     /gene="livF"
                     /locus_tag="OQM47_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1478693.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25836..26603)
                     /gene="livG"
                     /locus_tag="OQM47_000411"
     CDS             complement(25836..26603)
                     /gene="livG"
                     /locus_tag="OQM47_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1478694.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26600..27877)
                     /gene="livM"
                     /locus_tag="OQM47_000412"
     CDS             complement(26600..27877)
                     /gene="livM"
                     /locus_tag="OQM47_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1478695.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27874..28800)
                     /gene="livH"
                     /locus_tag="OQM47_000413"
     CDS             complement(27874..28800)
                     /gene="livH"
                     /locus_tag="OQM47_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1478696.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28848..29957)
                     /gene="livK"
                     /locus_tag="OQM47_000414"
     CDS             complement(28848..29957)
                     /gene="livK"
                     /locus_tag="OQM47_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1478697.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30381..30764
                     /gene="panM"
                     /locus_tag="OQM47_000415"
     CDS             30381..30764
                     /gene="panM"
                     /locus_tag="OQM47_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1478698.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30963..32066)
                     /gene="livJ"
                     /locus_tag="OQM47_000416"
     CDS             complement(30963..32066)
                     /gene="livJ"
                     /locus_tag="OQM47_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1478699.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32337..33191)
                     /gene="rpoH"
                     /locus_tag="OQM47_000417"
     CDS             complement(32337..33191)
                     /gene="rpoH"
                     /locus_tag="OQM47_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1478700.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33436..34494)
                     /gene="ftsX"
                     /locus_tag="OQM47_000418"
     CDS             complement(33436..34494)
                     /gene="ftsX"
                     /locus_tag="OQM47_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1478701.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34487..35155)
                     /gene="ftsE"
                     /locus_tag="OQM47_000419"
     CDS             complement(34487..35155)
                     /gene="ftsE"
                     /locus_tag="OQM47_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1478702.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35158..36654)
                     /gene="ftsY"
                     /locus_tag="OQM47_000420"
     CDS             complement(35158..36654)
                     /gene="ftsY"
                     /locus_tag="OQM47_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1478703.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36804..37400
                     /gene="rsmD"
                     /locus_tag="OQM47_000421"
     CDS             36804..37400
                     /gene="rsmD"
                     /locus_tag="OQM47_000421"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1478704.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37390..37659
                     /locus_tag="OQM47_000422"
     CDS             37390..37659
                     /locus_tag="OQM47_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1478705.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37662..38021)
                     /locus_tag="OQM47_000423"
     CDS             complement(37662..38021)
                     /locus_tag="OQM47_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1478706.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38162..38788
                     /locus_tag="OQM47_000424"
     CDS             38162..38788
                     /locus_tag="OQM47_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1478707.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38862..41060
                     /gene="zntA"
                     /locus_tag="OQM47_000425"
     CDS             38862..41060
                     /gene="zntA"
                     /locus_tag="OQM47_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1478708.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41162..41407)
                     /gene="tusA"
                     /locus_tag="OQM47_000426"
     CDS             complement(41162..41407)
                     /gene="tusA"
                     /locus_tag="OQM47_000426"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1478709.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41628..42293
                     /locus_tag="OQM47_000427"
     CDS             41628..42293
                     /locus_tag="OQM47_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1478710.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42366..42923
                     /gene="dcrB"
                     /locus_tag="OQM47_000428"
     CDS             42366..42923
                     /gene="dcrB"
                     /locus_tag="OQM47_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1478711.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42927..44144)
                     /locus_tag="OQM47_000429"
     CDS             complement(42927..44144)
                     /locus_tag="OQM47_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1478712.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44278..45327
                     /locus_tag="OQM47_000430"
     CDS             44278..45327
                     /locus_tag="OQM47_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1478713.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="OQM47_000431"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="OQM47_000431"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1478714.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="OQM47_000432"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="OQM47_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1478715.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="OQM47_000433"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="OQM47_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1478716.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="OQM47_000434"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="OQM47_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1478717.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="OQM47_000435"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="OQM47_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1478718.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="OQM47_000436"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="OQM47_000436"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1478719.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="OQM47_000437"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="OQM47_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1478720.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="OQM47_000438"
     CDS             complement(51522..51881)
                     /locus_tag="OQM47_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1478721.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="OQM47_000439"
     CDS             complement(51878..52153)
                     /locus_tag="OQM47_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1478722.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="OQM47_000440"
     CDS             complement(52212..53336)
                     /locus_tag="OQM47_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1478723.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OQM47_000441"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OQM47_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1478724.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="OQM47_000442"
     CDS             complement(56068..57135)
                     /locus_tag="OQM47_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1478725.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="OQM47_000443"
     CDS             complement(57501..59123)
                     /locus_tag="OQM47_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1478726.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="OQM47_000444"
     CDS             complement(59385..60974)
                     /locus_tag="OQM47_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1478727.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61359..62390
                     /locus_tag="OQM47_000445"
                     /pseudo
     CDS             61359..62390
                     /locus_tag="OQM47_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62477..62953)
                     /locus_tag="OQM47_000446"
     CDS             complement(62477..62953)
                     /locus_tag="OQM47_000446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478728.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62946..63710)
                     /locus_tag="OQM47_000447"
     CDS             complement(62946..63710)
                     /locus_tag="OQM47_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1478729.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63956..64717
                     /locus_tag="OQM47_000448"
     CDS             63956..64717
                     /locus_tag="OQM47_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1478730.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64824..65324)
                     /locus_tag="OQM47_000449"
     CDS             complement(64824..65324)
                     /locus_tag="OQM47_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478731.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65475..>66534)
                     /locus_tag="OQM47_000450"
     CDS             complement(65475..>66534)
                     /locus_tag="OQM47_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1478732.1"
                     /translation="KNSHDGSTISLSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSY
                     STVWRGITFSINWAKNDLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQ
                     GNNYQVSLSGQNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSE
                     NMKQLGANLSGGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAI
                     LPVQPYRRDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQK
                     EGKPIPFGAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQ
                     LPAKKSASGLYQIAATCL"
BASE COUNT        16171 a        17860 c        16327 g        16176 t
ORIGIN      
        1 ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga tatgatcagc
       61 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      121 ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct gacaatcgac
      181 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcaac
      241 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      301 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      361 cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag caccgacatg
      421 atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac cgttaagcgc
      481 aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct gttcagacag
      541 atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
      601 tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      661 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
      721 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
      781 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
      841 cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca gacgcttttt
      901 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
      961 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
     1021 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
     1081 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
     1141 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
     1201 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1261 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1321 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
     1381 gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
     1441 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
     1501 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
     1561 ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt attcctgacg
     1621 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
     1681 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga atttaccgag
     1741 gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg cgtcggtgtc
     1801 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
     1861 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
     1921 gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact ctttctggct
     1981 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2041 cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc ccaggccacc
     2101 gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
     2161 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
     2221 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2281 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2341 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
     2401 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2461 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2521 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2581 cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca gcgaccaggc
     2641 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2701 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2761 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2821 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2881 accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     2941 gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca gcaccaggtc
     3001 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3061 cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt ccaacgtatc
     3121 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3181 gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3241 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga ccgccgtaat
     3301 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
     3361 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3421 gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc cgcgcgccgc
     3481 cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat caggacgcca
     3541 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
     3601 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3661 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3721 gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3781 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3841 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3901 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     3961 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4021 ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac gcagcggcgc
     4081 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4141 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4201 acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
     4261 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4321 atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta agcttccagc
     4381 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4441 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4501 tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag atagtcggcg
     4561 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4621 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4681 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4741 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4801 aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca
     4861 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4921 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     4981 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt gatgcagtta
     5041 gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5101 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5161 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5221 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacaa
     5281 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5341 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5401 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5461 atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc tccaccaaag
     5521 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5581 cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg ttattgccat
     5641 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg gagagcaaca
     5701 acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca agagtaccgc
     5761 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5821 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5881 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     5941 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgtagccaga
     6001 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6061 ccacctgata accactagga gcgatatccc acggttcagc aattaacttc acctgcgaga
     6121 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6181 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
     6241 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6301 cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttat
     6361 cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6421 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6481 catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6541 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
     6601 gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag ttgatcatca
     6661 ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaagt
     6721 acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg
     6781 gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6841 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact
     6901 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     6961 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
     7021 ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag cgatgttcct
     7081 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7141 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
     7201 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg tcaggcttag
     7261 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
     7321 attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt tacctggctg
     7381 attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg caacgataat
     7441 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7501 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggcg
     7561 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
     7621 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
     7681 gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat agtaagcgcg
     7741 caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
     7801 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
     7861 cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct tcatgtagtc
     7921 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
     7981 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
     8041 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8101 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cacgataaat
     8161 catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
     8221 cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
     8281 ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8341 ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8401 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
     8461 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
     8521 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8581 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggaacc
     8641 caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
     8701 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8761 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
     8821 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
     8881 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     8941 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
     9001 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9061 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
     9121 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
     9181 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
     9241 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9301 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9361 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9421 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9481 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9541 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9601 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9661 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
     9721 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
     9781 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9841 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttcgacggtc
     9901 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
     9961 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10021 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    10081 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10141 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10201 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10261 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    10321 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10381 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    10441 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    10501 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10561 atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt gccagtggtt
    10621 ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac agggcgaatg
    10681 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10741 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10801 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca aagtcgcaag
    10861 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10921 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    10981 agttttattg atcctgatta tggatccgct cggaaacctg cctattttca tgtccgtact
    11041 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11101 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11161 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca ttaagatgat
    11221 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    11281 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11341 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    11401 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11461 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc agatgttcct
    11521 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt
    11581 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    11641 gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11701 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa cgtggctgac
    11761 aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac cagagtagtt
    11821 cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta cggtggcagc
    11881 cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa tcggcaggcc
    11941 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12001 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12061 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12121 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    12181 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12241 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt cgccgagatg
    12301 cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca
    12361 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12421 gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag gcaccaggaa
    12481 cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12541 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    12601 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
    12661 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat ataagatctt
    12721 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    12781 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12841 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12901 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    12961 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13021 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13081 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa tcacatcaaa
    13141 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13201 gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct gcatagcaaa
    13261 cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca gcggttcgcc
    13321 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    13381 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13441 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13501 gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga caatatcaga
    13561 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13621 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13681 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    13741 cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
    13801 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
    13861 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    13921 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatatgg
    13981 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14041 gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa tggcggtagt
    14101 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    14161 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14221 cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca tggattcgag
    14281 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct gataaccgtg
    14341 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14401 aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg gcgcacgatt
    14461 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14521 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14581 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14641 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14701 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    14761 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14821 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14881 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    14941 tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg cgttcagcgg
    15001 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
    15061 gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgca
    15121 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca ggcatgatcc
    15181 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15241 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    15301 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15361 ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15421 agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc caggagtcca
    15481 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15541 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15601 tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttaatt
    15661 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    15721 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    15781 cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    15841 accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
    15901 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    15961 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16021 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16081 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16141 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagtcc
    16201 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
    16261 tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg
    16321 attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
    16381 ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg cttccagcgc
    16441 gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca acttaacatc
    16501 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt aaatgggagc
    16561 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    16621 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16681 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16741 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    16801 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
    16861 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa taataatcat
    16921 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
    16981 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
    17041 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
    17101 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
    17161 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
    17221 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
    17281 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
    17341 tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc gataacgcgc
    17401 cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt aagaagtacg
    17461 cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa tagagaattt
    17521 gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat attaataccc
    17581 cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac cgtccggccc
    17641 aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct gaccttttgc
    17701 ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct cgtccggcaa
    17761 ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc cataatcgat
    17821 gctattcacc accatttgca gcaccgtggt gataatccgg ctaccggtaa ccagccaggt
    17881 tttaccgtct ttcaccacaa tggtcggcga catcgacgac agcgggcgtt tgttcggccc
    17941 gacggcgttg gcatcaccgc ccaccagccc gtaaacgttc ggtacgcccg gtttggcgga
    18001 gaaatcatcc atctggttat taagcagaat accgctctca cccgcgacaa tacccgtacc
    18061 gaaggtggtg ttcagcgtat aggtcaccgc caccgcgtta ccgtctttat ccactacaga
    18121 gtaatgggta gtttgattac tctcataagg cgcaagcttg ccagggcgaa tctcactgga
    18181 cggcttcgct tttttgatat caatttgatc ggcaatggat ttggcatagg ctttattggt
    18241 cagtgcctgc cacgggactt tgacaaaatc cgggtcgcca agatattccg agcggtcggc
    18301 gtaggcgtat ttctccgctt ctgccatgat ttgcatcgca tcggcgctgc caaagccgta
    18361 tttcttcata tcgaagtttt ccagaatatt gaggatttgt acgatatgga tcccgccgga
    18421 ggatggcggt ggcatggagt aaacctgata cccgcgataa tcgccgctta tcggagtgcg
    18481 ttcgaccgct ttataggccg ctaaatcttc tttagtgatc aagccaccgt ttttctgcat
    18541 ctcctgggcg atctgttccg caatcgtgcc tttatagaat tcgtccgggc cgttttcagc
    18601 aatcatctcc aggctctttg ccaggttcgc ctgcaccagc gtgtcgccct ttttcagcgg
    18661 ctcgccctct ttccagaaga tagctttact gttttcgtga ttcggcaaca cttcgctacc
    18721 gtaggttttg agatcgtcag ccagcgcgtc gttaacgata aaaccatcgc gtgccagttt
    18781 aaacgcgggc tgcacgactt tgttcagcgg catggtgccg tatttatcca gcgccagcga
    18841 gaaacctgct accgtacccg gtgtgccgga agccagatgc gaagtgagtg attttttgct
    18901 gtccgggttg ccctgatcat cgaggaacat atcgcgggtc gctttggcgg gtgccatttc
    18961 gcggaaatcg atagccgtgg tattgccatt tttcgagcgg attaacataa aaccaccacc
    19021 gcccagattc cctgcctgcg gatgcgttac cgccagcgcg tagcccaccg ccacggcggc
    19081 atcaacggca ttcccgccct ccttgaggat atccaccccc acctgagtgg cagtggcgtc
    19141 cacagacgct accattccct gtttcgcgcg taccggatgg aagacatctt cctccacacc
    19201 atacgagacg ggcggcgcag gaggcgcggc ggcggcgcta aaacaacttc ctgagagcag
    19261 agcagcaatg gccacccggc gtaaaaacgt cggttttatc atcgttattc tccagagatt
    19321 aaggggcaac cccagctaag cctggtatat aactctgaat taatcatcgt tttgtcggga
    19381 agcgagtaaa cttaaaggat atcttcagag gaggatacgc gatgaaacga cttctgattc
    19441 ttacggcact cctgccgttt gtcggctttg cacagcccat taatactctg aacaacccta
    19501 accagccggg gtatcagata cccagccagc agcggatgca aacccagatg cagactcagc
    19561 aaatccagca aaaagggatg ctgaatcagc aactgaaaac gcaaactcag ttgcaacagc
    19621 agcatttaga aaaccagata aacaataatt ctcagcgggt gttgcagtca cagccggggg
    19681 agcgaaatcc cgcccggcag caaatgctgc ccaacaccaa cggcgggatg ttaaacagca
    19741 accgtaatcc ggatagttcg ttgaatcagc agcatatgct gccggagagg agaaacggcg
    19801 acatgctgaa tcagcccagc acgccgcagc ctgacattcc gttgaaaact attgggccgt
    19861 aaagttcgga ccaatcacgt caatcgcatc ggtacagatg caatccacgc cccagcgcag
    19921 caactctgcc gcgtgctggg gtttattgac ggtataaacc agaatccgta atccggcgtc
    19981 tttcaactgc atcactcgcg ctttatcgag taacttatga ttgagatgaa tagagacgca
    20041 gcccagccgc gcggtcagtt cgcgccagtc gtcgcgccac tcatcaagca ataaaccacg
    20101 cggcagttcc ggtgccgcct gttgtgcagc ttctaaagca tcaatctcaa acgatgacag
    20161 cagcggcggc gtcataccgg cccacagttg gcgtgccgcc agcgccacca ttttgcccgt
    20221 taatggtcca gtgccagtgg tgggtttgat ttcgatattc gccatcatcc cgtgttcgcg
    20281 acagcgttcc gccacctgcg aaagcaacgg cagcggctca cctttaaacg ctttgctgta
    20341 ccaactgccc gcatccacgc gcagtaaatc ctgccagttc agttcacccg cgacgcccca
    20401 gccgttgctg gtgcgttcga gattgtcgtc atggagcagg aagatctcgc catctttcga
    20461 taacttcgcg tcaaattcga tcatcttatg accgtatttt gccccgacgt cgattgccgc
    20521 cagggtgttt tccggggcca gcttaccgcc gccacgatga gcgacgatgc ggggataagg
    20581 ccagttactc atactcgttg tcctgtttca ccatcaaaaa gatgcagctg attttccgcc
    20641 agatgcagcc acagcgtgct gcctgccgtc gggcgctcct gatgcgccag tcgcaccacc
    20701 agcttctgct tgcgagctta gcgcaatatg ttccgggcga atgccgaggg tcattttacg
    20761 cccggcatac tgacggtagc caccgtttag cggtagcacg ataccgccgt ccagttcgaa
    20821 atgcgtgcct tcattattca tgcggcctgt cagcaggttc atcgccgggc tgccgataaa
    20881 actcgccaca aacaggctgg cgggcttttc gtagacttcc accggcgtgc caatctgttc
    20941 ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct caacctgatc
    21001 gtgagtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca gttcaagacg
    21061 catctgtacg cgcagcttgg catcgaggtt agagagtggc tcatcaaaca agaacaccgc
    21121 cggatcgcgc acaatcgcgc gtcccatcgc cacacgctgg cgctgaccgc cggaaagctc
    21181 gcgcgggcga cgtttgagca gaccgtccag ctccagaatg cgcgccgctt ctttaacgcg
    21241 ctcggctatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca tgttttcttc
    21301 gacgctcata tgcggataaa gcgcgtaatt ctggaacacc atcgcaatcc cgcgatcttt
    21361 cggctccatt tcggtcacgc gctggtcgtt gatccagata tcgccttcgg tcaccctctc
    21421 cagcccggca accatacgca gcagcgtcga tttcccgcag ccagacggcc cgaccatcac
    21481 gataaattcg ccatccgcca catcaagggt cagcggttta atgacctagg ttttgccatc
    21541 ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca ctatcgacca
    21601 ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg atcagggtta
    21661 acaacatcgc cgccatcact gagttccatt ccgtggtgcc ttcgccagtg gcgatcatcc
    21721 ctttgatccc tgccacggtg gtgccgagat ccacgtcggt aataatcaac aacggccaca
    21781 aatactggtt ccagccgtaa ataaaggtga tcacaaacag cgccgccaga ttagttttgg
    21841 agagcggaaa aacgatgtcg caaaagaagc gcattggtga cgcaccgtcg atccgcgccg
    21901 cttccaccag ttcatccggc agcgtcataa agaactggcg aaacagaaac gtggcggtcg
    21961 ccgaggccat cagcggcagc gttaaaccgg cgtagctgtc gagcatcttc aggttggcga
    22021 tgacttccac cgtcgggaag atacgtactt caaccggcag catcagggtg ataaaaatca
    22081 tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat gccgagagca
    22141 tcgagacagt aattttgccg agcgtaatgc tgaacgccat cacaaagctg ttaagcaaca
    22201 tccgccagaa cggcgcgcta tttgtgccta ccccgttcac ccagatgttg tggatgtttt
    22261 ccagcagatg cgtgccgggg atgagcgtca tcggcgcggc gtagacggcc tgtttatcca
    22321 gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc ccgaggatca
    22381 gcatggtatg gctgaatatc gtcagccacg gacggttctc aatcattggt aacgcacctt
    22441 gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca tcagcaccat
    22501 cgactgtgcg gcagacgaag ccagatccag tccggtaaaa ccttcgcggt agatcttata
    22561 aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga tcaccgggaa
    22621 ggtgtcgaag aaggcataca ccaggttcac taccagcagg aagaaactca ccggggcgat
    22681 aagcggcagc gcaatcttaa agaagcggcg aatcggccct gcaccgtcga tggctgcggc
    22741 ttcgatcaac gaacggggaa tggattgcag cgcggcatag aagaacagaa agttgtagct
    22801 gatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc tattttgcgc
    22861 gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc gaccggggtt
    22921 aaacaggaag atccacaata cggcggcaac ggcgggagcc acggcatacg gcagcaacat
    22981 taaggtttgg tagaaacgac tgccgcgcac gatgtactcc accagcgccg caaagaacag
    23041 cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg tccagaagga
    23101 gtcgagataa tagctgtcat gaaacagcgt gacgaagtta tccagaccga caaactggct
    23161 ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc ccgcaggcca
    23221 gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcagataag gcagccagcg
    23281 cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg tgccggatgc
    23341 gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc atccggcatt
    23401 acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc ccacgctcaa
    23461 ctgcggtatc cagcgcctgc tgtggcgtct tcttaccggt ccacacgctc tcaagctctt
    23521 catccacaat cacgcggatc tgcggcatgt tgcccagacg aagccctttg gtgaacggca
    23581 acggcggctt gttcagcatc tgacgcgtcg cggtatccgc acctgggttt ttctcgtaaa
    23641 agccctgctc acgggtcagg tcatacgccg ctttggtaat tggcagataa ccggttttct
    23701 gatgccactc agcagcgttt tctggcttcg cgaggaagtc gagaaacttc gccacgccgg
    23761 tatacgtttc tttatctttg ccctgcatca cccacaggct ggctccgccg ataatggcgt
    23821 tttgtggcgc atctttcgca tcggcgtcgt aaggcatcat acctacgccg tagttaaatt
    23881 tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtggtcatc gcgcaatcac
    23941 cgttatagaa cttctcggtg gattcatcct tacgaccgac gtagctgaag tcgcccttct
    24001 tgttcatctc ctcgagcatg gcgatgtgtt tcacctgctc cggcttgttg aactccagca
    24061 ccgcgtccgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg ttccaggcgc
    24121 taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac ttaataccgg
    24181 aggctttcag ttttgcggaa tagtccgcca gatcctgcca ggttttcggc ggctgttccg
    24241 ggtctaaccc cgctttcttg aaggcgtctt tgttgtaata gagaacggaa gtcgagctgt
    24301 tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtagtaaccg gaaaccgtcg
    24361 gcacaaactg cgactcatcg aactgaatcc ctgcctcttt aaacacgtca tacaccggtt
    24421 taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc aaaatagccg
    24481 gagcgttgcc ggtacgaaat acggcaatcc ccgcgcttaa attctgttcg tagttgcctt
    24541 tataggtcgg tacaattttg taatccggat tttcggcgtt aaaacgttgg gccagagaat
    24601 ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc gtcactgcct
    24661 gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa tgtaacggtt
    24721 tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta tgctcgcggg
    24781 gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg taagatagta
    24841 aggtgtcaga aagatgacaa ggcggtgacg gcgtgggtga gggaaaatgg gagatggggc
    24901 acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc atcgtttcat
    24961 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc aatatattgc
    25021 aactcaccca gtaggcctga taagcgcagc gcatcaggca attttgcatt tgtcacctgt
    25081 ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct aaatacgcac
    25141 ttctcactgc ttcgttcgcc agcagcgcgt cgccggtatc ggagagcacc acatggccgt
    25201 tttccagcac gtagccgcga tccgccagct ttagcgcctg gttggcgttc tgctcgacga
    25261 ggaagatagt catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt tgctggatga
    25321 taatcggcgc aagaccgagc gatggctcat caagtaacaa caaacgcgga ttgctcatca
    25381 gcgcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgccc gcccgctgaa
    25441 cacggcgctc atgcagacgt ggaaacagtt catacaccca ctttatgcgc tcctgaaact
    25501 ggtcacgttc ggcaaagaag ccgcccatcg ccaggttctc ttccaccgtc atccgcgaga
    25561 agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc gccgtctgcc
    25621 agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc ggatcgccgc
    25681 ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgaga gtgacaatct
    25741 cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg tagtgggcgc
    25801 tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa ataggcacgg
    25861 atcacgtccg ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg cgtcccctga
    25921 ttgaccacat aaattcggtc tgaaatcccc atcaccagct tcatatcgtg ttcaatcaac
    25981 aaaatagtgg tgttgtgatg attgcgcagt tcggcaatca gctcatccag ctctttggtt
    26041 tcttttgggt taagacctgc cgccggttcg tcgagcatta aaatctccgg ctgcgtcacc
    26101 atgcagcggg caatctccag acggcgttgg tcaccatagg ccaggttact cgcctgacgg
    26161 ttggcgtgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc gagcgcttcg
    26221 ctctgggcgc gacggaaaga aggcgttttc aacaggccag agaacagccc ggttttcagt
    26281 tgctgatgct gcgccaccag caggttttca attaccgtca tttcacggaa cagacgcaca
    26341 tgctggaagg tgcgcaccac gcccatgcga gcaatttgct gccccggtaa accttcaaga
    26401 tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttgt agaaaccggt caggcagtta
    26461 aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatttc ctgcgggtac
    26521 agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat cagaccgtta
    26581 acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt ttcagcttca
    26641 gttgcgggcg cgtcatgggc agcaaaccct gcggacgcca gatcatcatc agcaccatca
    26701 aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaattcg cgcgacacca
    26761 ccagcagaac agccgccaga atcactgcaa attgcgagcc cataccgccg agcaccacta
    26821 tcgccagcac aaaggccgat tcagcaaagg tgaaggattc cgggctgaca aagccctgac
    26881 gcgccgcaaa cagcgttccg gcaaaaccgg caaatgcggc gctgatggta aaagcagtca
    26941 gcttgatacg gcgcgggctt aagcccagcg aacgacaggc gatctcgtct tcacgcaacg
    27001 cttcccacgc gcggcccagc ggcatccgca gcaggcggtt aatgacaaac aggcttaaca
    27061 ccaccagcag caacgccacc aggtagagga agataacgcg atcggacgga tcgtatttca
    27121 ggccaaagaa attactgaac gtgtcccagc cgccttcacg agcggtacgg ctgaactcga
    27181 gtccgaagag tgtcggtttc gggatctgac tgattccgtt cgggccgccg gtgatttcag
    27241 tgttattgag cagcaatatg cgcacaattt cgccgaaacc aagggtaacg atcgccagat
    27301 agtcaccgcg cagacgcagc accggaaaac cgagcaggaa acccgccgcc gctgccatca
    27361 gtccggcaat cggcaaacag gtccagaacc ccagaccgta atagtgattg agcagcgcaa
    27421 aagtgtaagc gccgatggcg taaaaaccgc cgtaccccag caccagcaga ccagaaagac
    27481 caacaaccac attcagcccg agaccgagga taatgtagat catggtcaga gtggcgatat
    27541 ccaccgtccc acgtgacacc ataaacggcc acgccaccac aagcaccagc agcgccacga
    27601 ggaacagctt ttgcttcacc gtggacccat caatcgcggg cagaataaac ttcggcccgg
    27661 aaacgctttt caaccctttc tggaaagccg gtcgcaaaag ctggaagaaa aagaccaccg
    27721 ccgtgccgat aaacatccac tgccaacgga tgtctgaagc cgtgtcgacc accagtttgg
    27781 tgccatccag ctccagttgt acgcccataa agacgcccgc caggacaaag aacatcgcgg
    27841 cagagagcag tgccattgca atatgcatcg gtttcatact ttctctacct ccgggcgacc
    27901 cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg agaccacatc
    27961 tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca ggatcagccc
    28021 gccaatcatc gcccctggaa tgctgccaat cccaccgagc accgcagcgg taaaggcttt
    28081 catcccggcc ataaagccga tataggggtt aatgacgcag tagaactgac cgagcagtac
    28141 gccagccact gccgccatcg ccgcgccaat aacaaaggtc aaagcaatca cccggtcggt
    28201 gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacgac aggcgcgccc
    28261 catacgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaagg tgacaatcca
    28321 gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatggc ccaccaccca
    28381 ctgaccgtta aacaggctcg gcagcgccac gtcgcgcgaa ccttcggtca ggctgacgta
    28441 gttttgcagg aagatggaca taccgattgc ggaaataagc gcaatcaggc gcttagagtt
    28501 acgcaccggg cggtaagcta cccgttcgat actccagccg taggcgctgg cgatgacgat
    28561 tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca tcatcatcag
    28621 cgcggcgatg atcataaatg agacgtagct gccaatcata taaacctcgc cgtgggcgaa
    28681 gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca gcgcgtaggt
    28741 actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact gctcagacat
    28801 aaggtaacct ttctaaaccc gcccgcattt tacgggcggt gggatgatca cttggctgcc
    28861 gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt aagatcgcct
    28921 ttttcatccc agttcagcgg cccaatcacg gtgtttgcac cgttagcttt taaatctttc
    28981 accagcgcca gcggctcatc gctgccggta cgctcaaggg cagttgccag agattgcact
    29041 gccgcgtaag tgatccagac ataaggcccg gacggatctt tcttgtctgc tttcagcgca
    29101 tcaacgatgc cctggtttgc cggatcctgg tcatagcgtt ttggcatagt gaccaacatg
    29161 ccttcggcgg catcaccggc aatgttcgac agcgatgcgt taccgacacc ttccggcccc
    29221 ataaactggg ttttcaggcc aacggaacgg gcttggcgca gcatctgccc catttccggg
    29281 tagtaaccgc cgtagtaaac gaagtcgata ttttcttttt tcaggcgggc gatcagcgcg
    29341 gagaaatctt tctccccggc ggtaataccg tcgaagaaga cgacgttggc gttagccgct
    29401 ttcagcccgt cctgcaccga acgcgccagc ccttcgccat actgttgttt gtcgtgaatg
    29461 atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt aggcccctgg
    29521 gaagagtcca gcccggcagt acgcataatg tgttgataac cgcgttgggt cagctccggg
    29581 ttggtcgctc ccggcgagat catcagaata ccttcgtctt cgtagatatc cgacgctggc
    29641 tgggtagagg aagaacacag atgaccaata acgtatttaa tgccgtcgtt aacgattttg
    29701 ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac gccaaccagc
    29761 ttgtcgccct taattccccc tttgacatta atgtctttaa tcgcctgacg cgcgccgtta
    29821 aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac ggcgacttta
    29881 atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc gatgatagtt
    29941 ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc tttgttttta
    30001 tgtgttaaca aatcagactg ttcttttttt atactgcact gtttttgcct gtctgattaa
    30061 ggggttagcg cagtattttg tgatgatagc gattaaaatc cctatttttc agtcgattaa
    30121 gaacagataa tattctgaat ttattgatag ataaacagaa aaaagtgcct ttgtcagcat
    30181 aaaataacgg cacaaagggc ggaataattc actatcattc aggggattat gctggacatt
    30241 tttcattctc taatgtttta actttgtaat tattgctgtt aaaaaattaa tcacctgcca
    30301 aaagaaataa aaaagagaaa gcctccgatt aaattatttc gctacactgg ttccactttt
    30361 gtgatttaca cgggttaccc atgaagctga ccatcattcg attagaaaac tttagcgacc
    30421 aggaccggat tgacctgcaa aagatctggc cggagtattc cccttcctct ttacaggttg
    30481 acgataacca ccgtatctac gccgcgcgtt ttaacgagcg tctgcttgcg gccgtgcggg
    30541 taaccttaag cggcaccgag ggagcactgg attctctacg cgtgcgggaa gtcacccgcc
    30601 gtcgcggtgt ggggcaatat ctgctggaag aggttttgcg taacaatcct ggcgtttcat
    30661 gctggtggat ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg tttatgcagg
    30721 cactgggatt tacggcacag cagggcggct gggagaagcg ttaatcgtca agttgatttc
    30781 aaaagtgatg accaacgaga aattgcctga tgcgctacgc ttatcaggcc tataatgtgt
    30841 gctgcaattt actgatttct ttggttcttg taggccggat aaggcgttta cgccgcatcc
    30901 ggcatgaagc aacgtattcg atattagcaa tttggcggca acccaaagtt gccgattaac
    30961 aattacttcg catcggtcgc cgtgccgttg gcgtgccagt caaatacgcc gaactcaaag
    31021 ccctttagat cgcctttctc atcccaggtc agtggcccca tcacggtatc cacggagttc
    31081 gcttttaggt atttggcgat ttcagccgga tcgtcagact gattcaggcc cgcctgcaaa
    31141 gattgcagcg cggcgtaggt ggtccagacg aatgcgccgc ttgggtcctg ttttttcgct
    31201 ttgatcgcat caacaatggg tttgttcgcc ggaacctgat cgtagttctt cggcttggtc
    31261 accagcagcc cttccgctga ttcgcccgca atgttagaca gcgaaacgtt agccacacct
    31321 tccggcccca taaactgagt tttcagccct gccgcgcgtg cctgacgcag gatttgcccc
    31381 atttccgggt gataaccgcc gtagtaaacg aagtcgatat tctctttttt cagacgcgcc
    31441 accagcgttg agaaatcttt ttccccggcg gtgataccat caaagaacac cacgttagca
    31501 ttgcctttct tcaggccgtc ctgcaccgct cgtgccagac cttcgccgta ttgctgtttg
    31561 tcgtgaacga tagcaatacg ctgcggtttc actttctcaa gaatatattt cgccgccgtc
    31621 ggcccctggt cggagtccag gccggtggtg cgcaggatca gctgatagcc acgggcggtc
    31681 agctccggcg cggttgccgc tggggtgatc attaaaatgc cttcgtcttc gtagatgtca
    31741 gacgcaggct gcgttgatga agaacagagg tgaccaatca catatttaat gccgtcgtta
    31801 acgactttgt tcgccaccgc aaccgcctgt ttcgggtcac aggcatcgtc atattttacg
    31861 atttgcagtt tgttgccttt aatgccgcct ttagcgttga tatccgcaac cgcctgctct
    31921 gcgccggtaa actcctggtc accgtactgc gcaaccggac cggacattgc acccacgacc
    31981 gcgactttaa tatcttctgc cagagccata ttgctgaatg ccagcgcgat acatcctgcc
    32041 agtaacgctt taccctttat gttcatcctg agaatcccca ttcttctggt tattacgtgt
    32101 gttgtgatgt tgttgttcag cactttattt cgttttatgc atgactaccc gtgctttagc
    32161 agcatactct gctaaaacat acccgatttt tatgatattg gaatagctat tttgacagtt
    32221 tattaacaat ctgcgtgggg attggcgttt tgccggaggg ggaattgatt attgtagagg
    32281 cccaaaaaac aaaaaccccg gactctcatc cagggttctc tgcttaatag cggaaattac
    32341 gcttcaatag cggcgcgaag ttttttcatc gcgttctttt ccagttggcg cacacgttca
    32401 gcggaaacac cgtaacggtc agccagttcc tgcaacgtgg acttgttgtc ttcgtccagc
    32461 cagcgcgcac ggatgatgtc ctggctgcgt tcgtccagac catgcatcgc gtcggtcaga
    32521 cggtttgccg cctgctcttc ccagttatca tcttcgatgc cgtcggcaaa gttagatgat
    32581 ttatcctgca gatagagcac cggtgccatc ggctggctgt cggaatcgtc gtcggaagac
    32641 aggtcaaagg tcatgtcctg tgccgccata cgtgattcca tctcacgtac gtctttgctg
    32701 gttacgccca gttcacgggc caccatttcg acttcatcct ggttaaacca gcccagacgc
    32761 tgcttggttt tacgcaggtt gaagaacagt ttgcgctgcg ctttggtggt cgcaactttg
    32821 acgatacgcc agttacgcag aacgtattcg tggatctctg ctttgatcca gtggacggcg
    32881 aaggagacca ggcgcacacc cacttccggg ttaaaacggc gcactgcttt catcaggccg
    32941 atgttacctt cctgaatcaa atccgcctgt ggcaggccat agcccgcata attacgagca
    33001 atatgaacaa caaaccgcag gtgagacagg atcagcgttt tagctgcttc cagatcgcca
    33061 tggtaatgca gcttttcagc cagcgcccgc tcctcgtcag ccgacaacat cggccacgcg
    33121 ttagctgccc ggatgtagga atccaggttg ccaactgggg ctaaagctaa actttgcatt
    33181 ttgtcagtca ttcaaatcct ctcaatcgat atcttctggc gcttcagtgg tagcaacaac
    33241 tgtgccaaag cttaagagca acgaggttat cattcactgt tttatcagac cgtgatttta
    33301 tccacaagtt caatgcaagc ttgtgaataa attacgcaca aaatgtgaca tagagatgaa
    33361 ataccgggaa gagacaacgg ggtctctttc cctgctacgg aacccattgc agggaaagag
    33421 tataacacgc ttttattatt caggcgtaaa gtggcgtaaa tgttgtaccg tggcaagcca
    33481 cgctgccacc cagccaatca tcgagcatac cagcagcaat agcaggcatt catcgaacga
    33541 taaaccattg atatcaaact tcgttccgaa aacctgtgcc acttccgcca ccgctgacga
    33601 caaacgcagt accagaattt ctgacaaaat taatgacaac aatgcgccag aaaatcccag
    33661 cagcgcaccg ccgtacagga acgggcgaag gatgaaacca tccgtcgcac caatcagttt
    33721 ctgcacgttg atggagtcac ggcgagcaaa gatgctcaga cgtacgctgt tgccgatgac
    33781 gaggaacact gccgccacca tcaacacgcc gatcatcgcc gaaacgcgcc caaccagccc
    33841 ggtcaacgcc gccagacggg caaaccagct gtcatccatc cgcacttcgt caataccgtt
    33901 aatctgcgtg atacgatcac gcagcgtatt gagtgattcc gtcccctgaa aatcgagttt
    33961 cgggatcacc accgccactg ccggaagcgg gttttcttcc agcatatcca gcgcaccacc
    34021 aaaaccagac cagttacgga actcgcccag tgcgtcttca cgagaaagat agttcacttt
    34081 ctccacgcct tgctcggcct gcaactgtgc caccacgccc gcagcggcgt catcgtccag
    34141 cgttttttgc aagtaaacag tgatttgcgg tgacggataa tactgcgtcg ccgcctgatt
    34201 aacgtttttg tacaccatat aacagacgct gggcagcgtc agggagatgg cgataaccat
    34261 caccgttaaa aacgtggcga acggtttgct tttcagatcc tgcaatgcgc cgtggaaggc
    34321 atagcgcacc tgttcgttga aaacgttggt tttgcgattt accggttttg gcgaggattt
    34381 cgcgcgtttc ggtgcgttac gaccgccgtc gcctgagccg ccgaccgatt tacggaagcg
    34441 atccagacgc ccaccaaact gccgaatatg attgattgca tcgcgcttat tcatggccca
    34501 cgcctccatg caagtgacca tcgctcaggg tgagcatgcg ataggaacgc cgtgaaatca
    34561 gattaatgtc gtgcgttgcc atcaatacgg ttaccccaac gcggttaaac tcttcaaaca
    34621 gacgtagaat cccttccgac aacgcgtcgt ccaggttacc ggtcggttcg tccgccagca
    34681 gtaccgcggg cttattcacc accgcgcggg caatgccaac acgctgttgt tcaccgccgg
    34741 aaagctgaat agggaagttc ttcgctttgt ccagtagccc gactttatcc agtgccgccg
    34801 acacccggcg acgaatatcg tcaccgctgg caccagcgat aatcagcggg atcgccacgt
    34861 tatcgtagac agtacggtcc atcagtagat ggtgatcctg gaaaatcatg ccaatctggc
    34921 ggcgcagaaa cggaacttca cggtttttca gacgcgtgat gtcatggccg ctaaaccaga
    34981 ttttcccggc gctgggccgc tcaatcccac agatcagctt caggagggta cttttccctg
    35041 cgccggaatg accggtcaga aacgccatct cacccggctg catatggaac gtaacgccct
    35101 gcagcgcctg tctcccaccg agataagcct tgctgacatg ttcaaagcga atcattgtta
    35161 atcctctcgg gcaaaaagtg cctctataaa gtcgtccgcc ttaaacggac gcaaatcctc
    35221 aatacgttcg ccgacaccaa tgtagcggat agggatacca aactggtcag ccaccgagaa
    35281 aattaccccg cctttcgccg tgccgtccag tttcgttagc gtgatgccgg ttaagccaac
    35341 agcttcgtgg aacagtttgg cctggcttac cgcgttctgc ccggtgctgg catcaatagt
    35401 cagcataact tcatgcggcg cttcaacgtc gagtttcttc atcacgcgga cgattttctt
    35461 caactcttcc atcagatgcg atttgttctg cagacgtccg gcggtatcgg caatcaggac
    35521 gtcgatatta cgcgctttag ctgcctgaat ggcgtcgaag ataacagagg cggaatccgc
    35581 accggtatgc tgggcaatca ccggaatatt gttgcgctga ccccagacct gaagctgttc
    35641 aaccgcagct gcacggaaag tatcgcccgc cgccagcatc accgatttac cctgctgctc
    35701 aaactgacgc gccagcttac caatcgtcgt ggttttaccc acaccgttga cgcccaccat
    35761 caggatcacg aacggcgttt tatcttcaac attcagcggc tcatcgactt tcgccagaat
    35821 ctcgcccatc tcttctttca gcaggccata gagcgcctcg gcatcacgaa gctgcttgcg
    35881 ggatgcgcct tccgtcagat tggtgataat tttacgtgtg gtttccacgc ccacatcggc
    35941 gatcaacagc tgttcttcca gctcttcaaa cagatcatcg tcgatttttt taccgcggaa
    36001 caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc gtttcaggcg
    36061 cgcgaagaag ccttctttgg tcggtttttc ttgctcctgg gcaatttctg caaccggctg
    36121 ctcttcttct gccggaggaa ctaccatcac cgcctcttct gccgcttcgg cagccagcgc
    36181 ctgtgcttcc agctcttcgt cggtgatttc ttctttagcc gcttcttctt ctgccgcttc
    36241 gacaatctct acggtttccg cttcagcctg ccactcttct ggcgaaacct cttcggcgtt
    36301 gacgtcttcc ggcaacggca gctcttcacg ttcgatagcc actggctccg gcgtttcttc
    36361 tacgaccggt tccggctgtg caacaacttc cgcttcaggc tgcgcttttt cactttcaac
    36421 aacctgttca gtgacttcca caacgtcggc agcaaaagtt tccgcctcgg cttcagtatg
    36481 cgcctgcggc tgctcttcaa cggcttgttc agaagccttc gcaggctctt gcgcctgaac
    36541 gatttcttct acaaccggtt gttcattctg aacttctgtc tctttttccg gggtctgctc
    36601 tttttgacca aagcccagcc aggaaaaaaa gccacgtctt ttttctttcg ccatttgcga
    36661 ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata gcagctaaaa
    36721 aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa gtatgtgtta
    36781 tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc ggccaaatcc
    36841 gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagc ccaggtctgc
    36901 gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg gtcattgttg
    36961 acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa gcgttatcgc
    37021 gctacgctgc gggggcaacg ttgattgaga tggatcgcgt ggtttctcag cagttaatta
    37081 agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac gcaatgtcat
    37141 tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg ttccgccgtg
    37201 gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct gacgaagccc
    37261 tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca aactggtcat
    37321 tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacac gaagcacaag
    37381 gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgcgtttg gggattttta
    37441 atcctcaatc tggtgcatcc cttcccgcgc ccgctaaata ttttcgttaa cgtggcgctg
    37501 atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac tttaccgaaa
    37561 gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg cgtgtttgaa
    37621 ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc gccaacaaag
    37681 ctaacaaagc gcgttccttt atagctcagc gtacctttat cgcccactgt cagggcgtgg
    37741 tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg tttgaagctt
    37801 gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga acggcgatcg
    37861 ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag cggagccata
    37921 tcattatcag ctttttcccg tcgctgttgc ataaaacgaa acgatgcggc gacgacaatt
    37981 aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc tcatcggaaa
    38041 atgcggaatt aagcataccc tgccagttat ggtgttgtca tccgtccacc ctcgccacta
    38101 aactggaagc aagaccgtag gcattctgct tacgaaaaaa taacgaattc aaggaactaa
    38161 gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg atgcatcgta
    38221 tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc tcctgctgtt
    38281 actggcctgg caagcaccga tgttcgacgc cattagctat ctggtgctgg cgggactgtg
    38341 cgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga tgtacgccat
    38401 cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc agatgacgct
    38461 ctctttcttc tggcctctgc cactggtgtt gctggttctg ggtgcgctgc tactggcgat
    38521 tatctggacg cggctggaag agtaccgttg gcctatctgc acgtttatcg gcatgacgct
    38581 ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc cggcgctctc
    38641 tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg ggagccacta
    38701 tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg ccggtcactt
    38761 cctcatcgtc cgctcactgt atctctgata aaacttgact ctggagtcga ctccagagtg
    38821 tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgaca cctgacaatc
    38881 acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aaccacggta cagaacgcca
    38941 acgactgttg ctgcgacggc gcatgctcca gctcgccaac gctctctgaa aacgtctccg
    39001 gcacccgcta tagctggaaa gtcagcggca tggactgcgc cgcctgtgcg cgcaaggtag
    39061 aaaatgccgt gcgccagctt gcaggcgtga atcaggtgca ggtgttgttc gccaccgaaa
    39121 aactggtggt tgatgccgac aatgacatcc gtgcccaggt tgaatctgcg gtgcaaaaag
    39181 cgggctattc cctgcgcgat gagcaggccg ccgacgagcc acaagcatcg cgcctgaaag
    39241 agaatctgcc gctgattacg cttatcgtga tgatggcaat cagctggggt ctggagcagt
    39301 tcaatcatcc gttcgggcaa ctggcgttta tcgcgaccac gctagttggg ctgtacccga
    39361 ttgctcgtca ggcattacgg ctgatcaaat ccggcagcta cttcgccatt gaaaccttaa
    39421 tgagcgtagc cgctattggt gcactgttta ttggcgcaac ggctgaagct gcgatggtgt
    39481 tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagccgc gcgcgtcagg
    39541 gcgttagcgc gttaatggcg ctgaaaccag aaaccgccac gcgcctgcgt aacggtgagc
    39601 gggaagaggt ggcgattaac agcctgcggc ctggcgatgt gattgaagtc gccgcaggtg
    39661 ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat gaaagcgccc
    39721 taaccggaga atccattccg gtggagcgcg cgacgggcga taaagttcct gcaggagcca
    39781 ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc agcgccattg
    39841 accggattct gaaactgatc gaagaagccg aagagcgccg cgcccccatt gagcggttta
    39901 tcgaccgttt cagccgtatt tatacgccag cgattatggc cgtcgccctg ctggtaacgc
    39961 tagtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa ggtctgacgc
    40021 tgctgctgat tggttgcccg tgtgcgttag ttatctcaac gcctgcggcg ataacctccg
    40081 ggctggcagc agcagcgcgt cgtggggcgt taattaaagg cggtgcggcg ctggaacaac
    40141 taggtcgtgt tacccaagtg gcgtttgata aaaccggtac gctgaccgtc ggtaaaccgc
    40201 gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg acactggcgg
    40261 cggcggtcga gcaaggcgcg acgcatccac tggcgcaagc catcgtacgc gaagcacagg
    40321 ttgctgaact cgccattccc accgccgaat cacagcgggc actggtcggg tctggcattg
    40381 aagcgcaggt taatggtgag cgcgtgttga tatgcgctgc cgggaaacat cccgctgatg
    40441 catttgctgg tttgattaat gaactggaaa gcgccgggca aacggttgtg ctggtagtac
    40501 gtaatgatga cgtgctgggt atcattgcat tgcaggatac cctgcgcgcc gacgctacaa
    40561 ctgccatcag tgaactgaac gcgctggggg ttaaaggagt gatcctcacc ggcgataatc
    40621 cacgcgcagc ggcggcaatt gccggggagc tggggctgga gtttaaagcg ggcctattgc
    40681 cggaagataa agtcaaagcg gtgaccaagc tgaatcaaca tgcgcccctg gcgatggtcg
    40741 gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt gctatgggta
    40801 gcggcacaga cgtggcgctg gaaaccgccg acgcagcatt aacccataac cacctgcgcg
    40861 gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc cagaacatca
    40921 ccattgcgct gggactgaaa gggatcttcc tcgtcaccac gcttttaggg atgaccgggt
    40981 tgtggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg aatgcgttaa
    41041 ggttgttgcg caggagataa ggcaaaccga tcgcaacatt gagcgcgatc ggtcccctcg
    41101 cccctctggg gagagggtta gggggtgagg aaaaggcggc atcgaagcca atcagcccct
    41161 atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct ttcgcaacca
    41221 gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtagtggcc ggatcgtcgg
    41281 cgataatcag caacgtttcg ccaggctgca tattgcgcac ggttttgcgc accatcatca
    41341 ccggttccgg gcagcgcagg ccaagcgcgt cgagtgtgtg gtcagggctg gaaaagagat
    41401 cggtcatttt cttctcatca cttaaaaaaa cggcgctagt ttacgccctg tgagtccgta
    41461 agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg taaagcagta
    41521 tcatgcggcg gctcgaaaaa agggtaagca cgttattatg ttaaggtaac agacgtgtcg
    41581 tacgtattgg gttccctcac cccaatggtt aatcaaaaag gtacaatatg aacgttttct
    41641 cgcaaactca acgctataag gcgttgttct ggttatcgtt atttcatctt ctggtgatca
    41701 cctccagtaa ctatctggtt cagcttcccg tctccatttt tggtttccat accacctggg
    41761 gcgcgtttag ctttccgttt atttttcttg ctaccgacct gaccgtgcgt atttttggcg
    41821 caccgctggc ccgacgcatt atcttcgcgg taatgatccc tgcgttatta atctcctacg
    41881 tcatctcgtc gctattctat atgggttcct ggcagggatt cggcgcactc gcccacttca
    41941 acctgtttgt cgcccgtatc gccaccgcca gtttcatggc ctacgcgctg gggcaaatcc
    42001 tcgacgtgca cgtttttaac cgcctgcgtc agagtcgccg ctggtggctg gcaccgacag
    42061 cgtccacact gttcggtaac gtcagcgaca cgctggcctt tttcttcatt gccttctggc
    42121 gtagcccgga tgcctttatg gctgaacact ggatggaaat cgcgctggtc gattactgtt
    42181 tcaaagtgtt aatcagtatc gttttcttcc tgccaatgta tggcgtatta ctcaatatgc
    42241 tgttgaaaag actggcagat aaatccgaaa tcaacgcttt gcaggcgagt taaaggttcg
    42301 ttatcagagt tgtgataaga tggatgaatg agccgttatg gccgtttatc gaaaggaaga
    42361 agtcaatgcg caatctggtt aaatatgtcg gaattggcct gctggttatg gggcttgcgg
    42421 cctgtgatga taaagacact aacgctacgg cgcagggttc ggttgcggaa agtaacgcta
    42481 ccgggaatcc cgtcaacctg cttgatggca agttaagttt ctcgctgcca gcggatatga
    42541 ccgaccagag cggtaagctg ggaacgcagg ccaataacat gcatgtctgg tccgacgcca
    42601 ccgggcagaa agcagtcatc gtcatcatgg gcgatgatcc gaaagaagat ctggcggtgt
    42661 tggcgaagcg tctggaagat cagcaacgta gccgcgatcc gcagctgcaa gtggtaacca
    42721 ataaagccat tgagctgaaa ggtcacaaaa tgcagcagtt agacagtatt atctccgcga
    42781 aaggccagac ggcgtactct tccgttattc tgggtaacgt gggtaatcaa ctgctgacca
    42841 tgcaaattac gctgccagct gacgatcagc aaaaagcgca aaccaccgca gaaaacatca
    42901 ttaatacgct ggttattcag taagttttaa gatgatgagg cggcctcagg gacgtgttcc
    42961 ggaggccgtt tttttaatcg ccacgtcagt aataacgcga ttgcgaccag tcccgccgcc
    43021 gccagataaa tcaccggtac gcccgcccag ctcatcacca gcccagccag tggtccagtc
    43081 acgccaagcg ataaatccat aaatacggtg taagttgcca gcgccgcccc ctgattttgc
    43141 tgcggaaccg cttttaccgc cactacaccc aatgccggga acaccagcga aaacccggcc
    43201 cccgccagta agacgccgat tttcgccatc cacggcatag tcgccacgcc aaccagtagc
    43261 aggccgatta tctcaacgct aaagcaaatc atcgctacgt ttaagccacc gatacggtta
    43321 atgccgttag ggaataacaa acgcgtaccg acaaacgcac agctaaacag cgtcagcgcg
    43381 aaagccgcac cgtcccaacc tttagcgtca taaaacagcg tgataaaggt ggcgatgacg
    43441 ccaaatccgg cggaagccag tgccagcgcc ataccgtaca gccagacgcg cccaagcacc
    43501 gcgcgaaacg gcagcggttt gcctttactg gcttttaccg tcggacgcgg gatcgccaac
    43561 aaaatggcca ccagcgccac gcccataatg attaacgcta acgcctgcaa gccgccccag
    43621 tgataaaaca cgacgcctaa cggcgcaccc atcgccatcg ccccgtaagt gacaatgccg
    43681 ttccacgaaa tcacccgccc gatatgcagc gagccaacca ctccaacgcc ccatagggtc
    43741 gatcccgttc cggcaaaact ttgcccaatc ccaaggatga cgcgccccag gcaaagtaat
    43801 aacaggctga tgacaggcag actggcggtt aatcctgccg tcagataccc cagaccgctc
    43861 aaaaagcagc cgcataaacc gaagacgaca atctttttgg gtcccagcaa atcggcgtaa
    43921 cgtccggcat gagggcggct cagcaaggtg gcgaaatatt gcaggctgat aaccaatcct
    43981 gcccagaagg cgctaaagcc catcacatca tggacatagc ccggtaatac agcgagcggc
    44041 aacccgatgg tgaggtagct ggcgaagtta aacatgacaa tggagacaat gcgcaaattc
    44101 aggcgcaatc cgtttagcgc gggttcggct acgggttcgg gcatgaggat caccacattt
    44161 ttacaacagt gtttcatttt taccatgtgc tgacgtgaaa atcagcagta agaatcagaa
    44221 tattgctggc gtgactcccg cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccgggc
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtacatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgtcaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg atcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggcag ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgacattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcac
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcacg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcacggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaagctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acacacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggagt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaaatg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcgcttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacggg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcaattttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagtgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg catttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt ttcattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgatcgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagccat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtaatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg ggaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaatattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattacat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg attttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagc
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat ttatttatca
    61201 tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat agttatgcta
    61261 tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta tcattaatgc
    61321 acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat atcgggtagc
    61381 ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt ggctttgtgc
    61441 tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc catatgcctg
    61501 gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt taatacgttt
    61561 tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat gatatggggt
    61621 tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg ccatgttatt
    61681 tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc cggtcacttt
    61741 ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt acaggcttat
    61801 tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct gctcggtttc
    61861 atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt cgccttgggt
    61921 taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat caaacacgta
    61981 atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt ctcggctcag
    62041 tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc cgactggctc
    62101 ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca aaagtctggc
    62161 tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg atgataccgg
    62221 tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc gcacaaactg
    62281 agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta tgttttacat
    62341 tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa aatttacata
    62401 atcccagcgt tattcatatc ttaacgctgg ttttttacga acttagtgaa ataatgtaga
    62461 atataaaatt ataattttat attggagcac atgtgttaat gatcaacccc gggatactaa
    62521 aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat
    62581 cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc gaaactttgg
    62641 ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa aaatacgaat
    62701 gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac
    62761 catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga tctgttttag
    62821 catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca cgagccttcc
    62881 agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata aaatacattt
    62941 tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc attagaggat
    63001 tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat actatttaca
    63061 tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat ttcagcggcc
    63121 tctatttgag atttagcgag gccaatctcg ttaagattct ctgaaagata taaatcacag
    63181 gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt atctttaaaa
    63241 gccacggcaa acatttcaat aataacaatt actaccacaa gcgaaatcaa aatgcgaaaa
    63301 agaggaatac tccttttgcg ctctactttt tcaacaacat cttcttgtaa atgagaatca
    63361 tcataagatt gtttacttat tgttatgttt tcactgatgt aaaagcctac tttgggtatc
    63421 gtaagaatca cattttcttc aacaccaata tttttaaaac ttttgcgtaa cagggatata
    63481 tattggttca atgtgttgtt tgacagatca aatgaaaatc tctccaaaac gctatcaatg
    63541 attgagttcc tgtcaaccgc tccaccatta ctttccagta acactgccaa aatacacccc
    63601 gccgtagctg gaaaatttat ctcatcctgg gtatttacat tcgagagcga gcatttgttc
    63661 ggatcaaaaa taatcgcgtc attgataatg aaaaaacatc tccgctccat gcaggcaaca
    63721 acacaaaata agattttgtc attttagcct attcctggtg gaactgacac aagaaactaa
    63781 aatacaaata taaccctgaa aaagtcatat aaaacttata tccacagaat aaaaaatcaa
    63841 acatgtcagt tttatcaata taaagtatcg caaggcgaaa agtgatgcaa gaatactcct
    63901 aagtaaatga aagcgcacca catgaaatta tcaacacatg taatttcatc attaggtgct
    63961 atcgaaaata actcaggagc tacttaagat gaataatgtc ttactcatga gacccgatga
    64021 ctcagaattt atgatacctg atgactggtc agggctaaac gcaaaaggca ttacatacga
    64081 acaagataat agagtgacag agtcttttct gccttttaaa tctttttatt gttcaccggt
    64141 gtgcacgaca gttgaactca tctcacaaaa taattgtttt gctatgggta tccgctacct
    64201 tctggaaaag aaaagagact taaggctaaa tcactttaaa gccttctctc ataaatgcga
    64261 gacaaaacct gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt atagtatatt
    64321 ctcacgcatt gttgaagaaa atataatgaa aacgatgaca cctatcgtca tcattactgg
    64381 gcatcactca gaagatcttt gttctctggc atcgaaactg ggaggagtta aaataatatc
    64441 accgacagaa aagattgcaa aaatagaaaa cgctatcaat gatgctttta acagcaagcc
    64501 taaaaaaata agatttttaa cagatatact ctctcctcaa caaattgaga ccatgaaaat
    64561 ggttgcgcag ggtagatccg ttaatgagat ttcagccgca atgaatatta gcatcaagac
    64621 cgtgtatgca cataaagttc gtgctatcaa taagctggga attaagaaaa aagtttatga
    64681 agcgcttttt tataaagcgc taattcagtt caactgatgt aaaaatcacc ctcgcttaat
    64741 aaataatata ttctatgtct ttttttataa gttaaaaaag gcgggatatc aaatcccgcc
    64801 atgaaattaa ctaatcatct gcattattct gcgtagatat aagcctgggt acggatggtg
    64861 taagagtctg gctcaacaac ctgttctttc atatgggaaa cgacgcgata ggcaccacct
    64921 gttccagtat ttttcagcat catataacgc ttaccgccaa tctcttcgga ggcagctgaa
    64981 ggaggatcct gctcaagcgt tgtcagggaa actttcagtt tatggtccgg gttattgtca
    65041 ccagaaatta ctgcatccgc tgaagtactg ctctccgcat aaatatcatc cgggaaagtt
    65101 acagcatagt cgtggctctg cccatcagat gcgccaacaa tacctgttgc caaaggcgtc
    65161 catgcagtta acgtaccgct atatacccct ttcactggtt gaatggtatt attaacaacg
    65221 ataggcttgg tcaaagtaag attagctgac gcttcagtac cctggcttac taaagttccc
    65281 gcctgagaag caaaagctgt ccccgccagc atgattgcta acaatgtttt cttcattata
    65341 ttttctccat gattttcatt tacactgcaa aatgcagttt ctttctttgc atttacgtgc
    65401 tatcgcgaaa atgcttccac caaataatgg aaacatcttc acaaaaataa attatgagtt
    65461 tcttaattta gaaactataa acatgtcgcc gctatctggt ataaccctga tgcacttttc
    65521 ttcgcaggaa gttggtattt tgcgatacag gattgatctt tagaataacc ccatttcaca
    65581 ttaaactcgc cttccgcact caagccagaa acataagcaa taccatcttc acctacaatg
    65641 ccggccatat tttcagtgtc tttgctataa ctaatcacag cgccgaaggg gatgggttta
    65701 ccctctttct gttttaaggt tactaatcct ttatccccgc tcttcactgc caatgtcgca
    65761 ggcacaacgg ctccagctgt cggtacaacc aattgactag tttgtggtaa gtcgtaattc
    65821 ttacctatcg ttttttcatc cagaataaca tcgtctcttc ggtatggctg cactggcagt
    65881 attgcatacc cacgaaaatc agtactcagt cccggccagc cattaacgtt aacccccgtt
    65941 gctccgggtg cctctataag ggctaatgtc ccatccacat tttgaccaaa tgtaagtcca
    66001 tcccttgtaa taagtatccc accgcttaaa tttgcaccta gctgtttcat gttttcgctg
    66061 gagctgtaat ggctactaaa ctgaccatat gatccatttt tgccaacttg cacaatgttc
    66121 atcgtgctct tcggttgtga ctgatgagcg ttagttatat ttgtcgctac atcccaccat
    66181 acaccaccac tattctgacc tgataatgaa acctgatagt tattaccctg ctctcccgaa
    66241 cgactcatct gtgaagacgc ataaatttga ttttcgtcac ccagcgataa tggcactgaa
    66301 atacccacgc ttaatttccg atctactttc catttatgcg atgtcagcaa gtcgtttttt
    66361 gcccagttaa tggagaatgt tatgcctctc cataccgtac tataactcag gctggtagac
    66421 cgcgtgtttc ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt caaattcccg
    66481 atattaccaa ggggctggga taaggatatc gtagagccat cgtgactatt ctta
//
LOCUS       DAJVPT010000007        65680 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVPT010000007 DAJVPT010000000
VERSION     DAJVPT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(120..599)
                     /gene="folK"
                     /locus_tag="OQM47_000451"
     CDS             complement(120..599)
                     /gene="folK"
                     /locus_tag="OQM47_000451"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1478733.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(596..2014)
                     /gene="pcnB"
                     /locus_tag="OQM47_000452"
     CDS             complement(596..2014)
                     /gene="pcnB"
                     /locus_tag="OQM47_000452"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1478734.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2053..2979)
                     /gene="gluQRS"
                     /locus_tag="OQM47_000453"
     CDS             complement(2053..2979)
                     /gene="gluQRS"
                     /locus_tag="OQM47_000453"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1478735.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3016..3471)
                     /gene="dksA"
                     /locus_tag="OQM47_000454"
     CDS             complement(3016..3471)
                     /gene="dksA"
                     /locus_tag="OQM47_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1478736.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3649..4353)
                     /gene="sfsA"
                     /locus_tag="OQM47_000455"
     CDS             complement(3649..4353)
                     /gene="sfsA"
                     /locus_tag="OQM47_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1478737.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4368..4898)
                     /gene="thpR"
                     /locus_tag="OQM47_000456"
     CDS             complement(4368..4898)
                     /gene="thpR"
                     /locus_tag="OQM47_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1478738.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4972..7401
                     /gene="hrpB"
                     /locus_tag="OQM47_000457"
     CDS             4972..7401
                     /gene="hrpB"
                     /locus_tag="OQM47_000457"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1478739.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7597..10131
                     /gene="mrcB"
                     /locus_tag="OQM47_000458"
     CDS             7597..10131
                     /gene="mrcB"
                     /locus_tag="OQM47_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1478740.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10351..12594
                     /gene="fhuA"
                     /locus_tag="OQM47_000459"
     CDS             10351..12594
                     /gene="fhuA"
                     /locus_tag="OQM47_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1478741.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12645..13442
                     /gene="fhuC"
                     /locus_tag="OQM47_000460"
     CDS             12645..13442
                     /gene="fhuC"
                     /locus_tag="OQM47_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1478742.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13442..14332
                     /gene="fhuD"
                     /locus_tag="OQM47_000461"
     CDS             13442..14332
                     /gene="fhuD"
                     /locus_tag="OQM47_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1478743.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14329..16311
                     /gene="fhuB"
                     /locus_tag="OQM47_000462"
     CDS             14329..16311
                     /gene="fhuB"
                     /locus_tag="OQM47_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1478744.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16346..17626)
                     /gene="hemL"
                     /locus_tag="OQM47_000463"
     CDS             complement(16346..17626)
                     /gene="hemL"
                     /locus_tag="OQM47_000463"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1478745.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17851..19272
                     /gene="clcA"
                     /locus_tag="OQM47_000464"
     CDS             17851..19272
                     /gene="clcA"
                     /locus_tag="OQM47_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1478746.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19296..19361
                     /locus_tag="OQM47_000465"
     CDS             19296..19361
                     /locus_tag="OQM47_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478747.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19354..19698
                     /gene="erpA"
                     /locus_tag="OQM47_000466"
     CDS             19354..19698
                     /gene="erpA"
                     /locus_tag="OQM47_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1478748.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19745..20368)
                     /locus_tag="OQM47_000467"
     CDS             complement(19745..20368)
                     /locus_tag="OQM47_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1478749.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20406..21206)
                     /gene="btuF"
                     /locus_tag="OQM47_000468"
     CDS             complement(20406..21206)
                     /gene="btuF"
                     /locus_tag="OQM47_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1478750.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21199..21897)
                     /gene="mtnN"
                     /locus_tag="OQM47_000469"
     CDS             complement(21199..21897)
                     /gene="mtnN"
                     /locus_tag="OQM47_000469"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1478751.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21981..23498
                     /gene="dgt"
                     /locus_tag="OQM47_000470"
     CDS             21981..23498
                     /gene="dgt"
                     /locus_tag="OQM47_000470"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1478752.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23628..25052
                     /gene="degP"
                     /locus_tag="OQM47_000471"
     CDS             23628..25052
                     /gene="degP"
                     /locus_tag="OQM47_000471"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1478753.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25207..26363
                     /gene="cdaR"
                     /locus_tag="OQM47_000472"
                     /pseudo
     CDS             25207..26363
                     /gene="cdaR"
                     /locus_tag="OQM47_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26452..26838)
                     /locus_tag="OQM47_000473"
     CDS             complement(26452..26838)
                     /locus_tag="OQM47_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1478754.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27152..27976)
                     /gene="dapD"
                     /locus_tag="OQM47_000474"
     CDS             complement(27152..27976)
                     /gene="dapD"
                     /locus_tag="OQM47_000474"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1478755.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28007..30679)
                     /gene="glnD"
                     /locus_tag="OQM47_000475"
     CDS             complement(28007..30679)
                     /gene="glnD"
                     /locus_tag="OQM47_000475"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1478756.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30741..31535)
                     /gene="map"
                     /locus_tag="OQM47_000476"
     CDS             complement(30741..31535)
                     /gene="map"
                     /locus_tag="OQM47_000476"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1478757.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31903..32628
                     /gene="rpsB"
                     /locus_tag="OQM47_000477"
     CDS             31903..32628
                     /gene="rpsB"
                     /locus_tag="OQM47_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1478758.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32886..33737
                     /gene="tsf"
                     /locus_tag="OQM47_000478"
     CDS             32886..33737
                     /gene="tsf"
                     /locus_tag="OQM47_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1478759.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33884..34609
                     /gene="pyrH"
                     /locus_tag="OQM47_000479"
     CDS             33884..34609
                     /gene="pyrH"
                     /locus_tag="OQM47_000479"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1478760.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34901..35458
                     /gene="frr"
                     /locus_tag="OQM47_000480"
     CDS             34901..35458
                     /gene="frr"
                     /locus_tag="OQM47_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1478761.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35550..36746
                     /gene="ispC"
                     /locus_tag="OQM47_000481"
     CDS             35550..36746
                     /gene="ispC"
                     /locus_tag="OQM47_000481"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1478762.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36935..37693
                     /gene="ispU"
                     /locus_tag="OQM47_000482"
                     /gene_synonym="uppS"
     CDS             36935..37693
                     /gene="ispU"
                     /locus_tag="OQM47_000482"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1478763.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37706..38563
                     /gene="cdsA"
                     /locus_tag="OQM47_000483"
     CDS             37706..38563
                     /gene="cdsA"
                     /locus_tag="OQM47_000483"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1478764.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38575..39927
                     /gene="rseP"
                     /locus_tag="OQM47_000484"
     CDS             38575..39927
                     /gene="rseP"
                     /locus_tag="OQM47_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1478765.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39957..42389
                     /gene="bamA"
                     /locus_tag="OQM47_000485"
     CDS             39957..42389
                     /gene="bamA"
                     /locus_tag="OQM47_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1478766.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42511..42996
                     /gene="skp"
                     /locus_tag="OQM47_000486"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42511..42996
                     /gene="skp"
                     /locus_tag="OQM47_000486"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1478767.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43000..44025
                     /gene="lpxD"
                     /locus_tag="OQM47_000487"
     CDS             43000..44025
                     /gene="lpxD"
                     /locus_tag="OQM47_000487"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1478768.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44130..44585
                     /gene="fabZ"
                     /locus_tag="OQM47_000488"
     CDS             44130..44585
                     /gene="fabZ"
                     /locus_tag="OQM47_000488"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1478769.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44589..45377
                     /gene="lpxA"
                     /locus_tag="OQM47_000489"
     CDS             44589..45377
                     /gene="lpxA"
                     /locus_tag="OQM47_000489"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1478770.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45377..46525
                     /gene="lpxB"
                     /locus_tag="OQM47_000490"
     CDS             45377..46525
                     /gene="lpxB"
                     /locus_tag="OQM47_000490"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1478771.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46522..47118
                     /gene="rnhB"
                     /locus_tag="OQM47_000491"
     CDS             46522..47118
                     /gene="rnhB"
                     /locus_tag="OQM47_000491"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1478772.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47155..50637
                     /gene="dnaE"
                     /locus_tag="OQM47_000492"
     CDS             47155..50637
                     /gene="dnaE"
                     /locus_tag="OQM47_000492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1478773.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50650..51609
                     /gene="accA"
                     /locus_tag="OQM47_000493"
     CDS             50650..51609
                     /gene="accA"
                     /locus_tag="OQM47_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1478774.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51708..53849
                     /gene="ldcC"
                     /locus_tag="OQM47_000494"
     CDS             51708..53849
                     /gene="ldcC"
                     /locus_tag="OQM47_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1478775.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53906..54295
                     /locus_tag="OQM47_000495"
     CDS             53906..54295
                     /locus_tag="OQM47_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1478776.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54360..55658
                     /gene="tilS"
                     /locus_tag="OQM47_000496"
     CDS             54360..55658
                     /gene="tilS"
                     /locus_tag="OQM47_000496"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1478777.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55707..55967)
                     /gene="rof"
                     /locus_tag="OQM47_000497"
     CDS             complement(55707..55967)
                     /gene="rof"
                     /locus_tag="OQM47_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1478778.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55954..56154)
                     /locus_tag="OQM47_000498"
     CDS             complement(55954..56154)
                     /locus_tag="OQM47_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1478779.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56320..56865
                     /locus_tag="OQM47_000499"
     CDS             56320..56865
                     /locus_tag="OQM47_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1478780.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56862..57284
                     /gene="arfB"
                     /locus_tag="OQM47_000500"
     CDS             56862..57284
                     /gene="arfB"
                     /locus_tag="OQM47_000500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1478781.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57298..58008
                     /gene="nlpE"
                     /locus_tag="OQM47_000501"
                     /gene_synonym="cutF"
     CDS             57298..58008
                     /gene="nlpE"
                     /locus_tag="OQM47_000501"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1478782.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58163..58987)
                     /locus_tag="OQM47_000502"
     CDS             complement(58163..58987)
                     /locus_tag="OQM47_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1478783.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59040..60758)
                     /gene="proS"
                     /locus_tag="OQM47_000503"
     CDS             complement(59040..60758)
                     /gene="proS"
                     /locus_tag="OQM47_000503"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1478784.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60869..61576)
                     /gene="tsaA"
                     /locus_tag="OQM47_000504"
     CDS             complement(60869..61576)
                     /gene="tsaA"
                     /locus_tag="OQM47_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1478785.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61573..61977)
                     /gene="rcsF"
                     /locus_tag="OQM47_000505"
     CDS             complement(61573..61977)
                     /gene="rcsF"
                     /locus_tag="OQM47_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1478786.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62095..62910)
                     /gene="metQ"
                     /locus_tag="OQM47_000506"
     CDS             complement(62095..62910)
                     /gene="metQ"
                     /locus_tag="OQM47_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1478787.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62950..63603)
                     /gene="metI"
                     /locus_tag="OQM47_000507"
     CDS             complement(62950..63603)
                     /gene="metI"
                     /locus_tag="OQM47_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1478788.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63596..64627)
                     /gene="metN"
                     /locus_tag="OQM47_000508"
     CDS             complement(63596..64627)
                     /gene="metN"
                     /locus_tag="OQM47_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1478789.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64815..65390
                     /gene="gmhB"
                     /locus_tag="OQM47_000509"
     CDS             64815..65390
                     /gene="gmhB"
                     /locus_tag="OQM47_000509"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1478790.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15532 a        16277 c        18093 g        15778 t
ORIGIN      
        1 atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact aaaattttta
       61 ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat ataaataaat
      121 taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa cgtctcccca
      181 tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa tccacgattc
      241 ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt accaaacagc
      301 atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac acgaccctgt
      361 tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga agtttcaagc
      421 gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg ggtgcggtaa
      481 aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt cagggcagca
      541 ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc cactgtcatg
      601 tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga cgcggtgacg
      661 gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa acctggaact
      721 caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca gctcgcaagg
      781 ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat gcgcgtttac
      841 cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac gtcgtcagac
      901 gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc gccagcgcga
      961 aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc tccagcagtg
     1021 ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg ttatggatac
     1081 gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg tcgccatttt
     1141 ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat tcacacaaca
     1201 gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct tcaaacaggc
     1261 gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct gcggtttccg
     1321 ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata cgtaccggat
     1381 cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc ttcagatcct
     1441 tcataccacc aacgtaatca cggacggtaa aatccgctac gctgtaatac aggctgttga
     1501 tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca
     1561 acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc
     1621 cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc agacggaaac
     1681 ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag
     1741 tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa
     1801 ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg gcattttcac
     1861 tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc tgtggacggg
     1921 cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa aaattagcga
     1981 ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg
     2041 gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg ccgactcggg
     2101 cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga cgctgaaatc
     2161 ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta gtaccgggcg
     2221 tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag cgccttgtgg
     2281 attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa gctggtacag
     2341 cgggatttgc cttactgttg gttcaatcag atcagcccca cgcactattt ctgtaacgcc
     2401 ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat cacggcgatg
     2461 aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc gcagcaggtc
     2521 agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt ctggtccatg
     2581 atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt
     2641 acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt cacgataggc
     2701 gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt aatgttccag
     2761 ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga tatcttctat
     2821 gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga gcgcggcgat
     2881 cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa tatactgtgt
     2941 gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg
     3001 atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca gcgttttgca
     3061 gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc
     3121 gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct tctcgatctt
     3181 tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact cttcttcctg
     3241 ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt
     3301 gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac ggaagtgcgc
     3361 cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt ccaccccagc
     3421 gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca tgttgcttct
     3481 ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct ataaatagca
     3541 gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt gaggaaaagc
     3601 gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact acaatgtaac
     3661 cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt aagccagaat
     3721 ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat cgatgtggcg
     3781 cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaaaataa ccgcacgctg
     3841 gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc
     3901 atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt caatatagca
     3961 gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg agccgtattt
     4021 tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat taaggatagc
     4081 ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc cgctctggct
     4141 ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca
     4201 gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc agtgtagcgt
     4261 taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag
     4321 cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta ttgcgttagc
     4381 gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg
     4441 gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt caccgcctcg
     4501 ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg aaaacaaccg
     4561 ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg tggaggctga
     4621 cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt
     4681 gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc tttctctgcg
     4741 ctcacttcgc ctaaaaatgc cagagtcagg tgcaaattat cggcggcgac cggacgtccc
     4801 gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcggat ttctgcaggt
     4861 aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg gttatgaatt
     4921 accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg
     4981 ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc gccgcaggta
     5041 ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca actgctggcg
     5101 catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac
     5161 gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt tggctaccgg
     5221 atgcgtgcgc aaaactgcgt cgggctgaat acccgcctgg aagtggttac cgaaggcgtg
     5281 ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt gatccttgat
     5341 gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa
     5401 caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga caatgaccgc
     5461 ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc gtttccggtt
     5521 gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt tgcggtagcc
     5581 accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc tggcgtcgga
     5641 gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt ggtgctctgc
     5701 ccgctgtatg gcgtgttgtc actgaacgat cagcgaaaag cgatcctccc ggcaccgcaa
     5761 gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac catcgaaggt
     5821 attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc gcgcacgggg
     5881 cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg tgccgggcgc
     5941 gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca agcagaacgc
     6001 gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt gctgatggaa
     6061 ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca accgccaacg
     6121 gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga gggtgaacgg
     6181 cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt agcggcaatg
     6241 ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc cgccattctc
     6301 gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg caatcaacca
     6361 gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg cggtgaggca
     6421 gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat tgctcatcgc
     6481 cgtgggcaag atggacgcta tcaactggca aacagcatgg gagcgatgct cgatgccgac
     6541 gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg cagcgcctcg
     6601 ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt acaacgctgt
     6661 ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg tacgctgaaa
     6721 gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct ggcgaaaccg
     6781 tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg tttaagcgtg
     6841 ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg
     6901 ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac gctggaaacg
     6961 tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc actcgacatt
     7021 tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga tagtgaattg
     7081 cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca tgaagataac
     7141 ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa tccgacgatc
     7201 gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag gccgctgcaa
     7261 atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca aaaggagatg
     7321 aaagggcgtt atcccaaaca cgtctggccg gacgacccgg caaacacggc accgacgcga
     7381 cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag agggccgggg
     7441 tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag ggagagggga
     7501 ccgatcgagc gcaaattttg agagatatct tcttctgtct tgtaacagaa gaacagaaaa
     7561 tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga ccgcgagcca
     7621 attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac
     7681 gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc gatgccgcgc
     7741 aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg gctatggcta
     7801 ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat
     7861 caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc agtttatggc
     7921 cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt gaagctgctg
     7981 gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag
     8041 gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa agaagggcag
     8101 gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa tatggagaac
     8161 aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat ctcctcgcca
     8221 aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct ggtggatact
     8281 ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct ctactccatt
     8341 gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc gagtacgctg
     8401 acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg gcgtaaagcg
     8461 aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg tattcttgag
     8521 ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg cggcttcccg
     8581 ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga ccagcaggcg
     8641 ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa cccgaaactg
     8701 gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa
     8761 gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg tggggtgatc
     8821 tctcctcagc cagcctttat gcagctggtg cgtcaggagt tgcaggcaaa actgggcgat
     8881 aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt ggcccaggac
     8941 gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg taagttgagc
     9001 gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc
     9061 ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt
     9121 ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa aatctatcgc
     9181 ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg ccaggtctgg
     9241 tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct ggtggatgcg
     9301 ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg gctgcctgcg
     9361 gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc ggttccggca
     9421 atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt ccagaccatc
     9481 gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc ggaagatggt
     9541 aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca ggcggcgtat
     9601 ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca gcttggggcg
     9661 aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa cgtagatacc
     9721 tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg tgataacaac
     9781 cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg ttatctggct
     9841 aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga tatgggcgtg
     9901 gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc
     9961 gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg caatccgttt
    10021 gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca agagcagaaa
    10081 gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta acatctaagc
    10141 gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta agatattaac
    10201 cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg cgtaccgctt
    10261 gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc tcgtttacgt
    10321 tattattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc tcagccaaaa
    10381 cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat gtctgtttat
    10441 gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc acctgcgccg
    10501 caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc taccggcact
    10561 aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac cgccgaagag
    10621 atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct
    10681 gttggtacgc gtggcgcatc caacacctat gaccacctga tcattcgcgg ttttgcggca
    10741 gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa cttctataac
    10801 gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc
    10861 gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa gcgtccgacc
    10921 accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt ccagactggt
    10981 tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct gaccggtctt
    11041 gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc tattgcaccg
    11101 gcgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta cttccagaac
    11161 gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga gccgctgccg
    11221 aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac ctattctcgt
    11281 aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt tactgtgcgt
    11341 cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc
    11401 tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa
    11461 ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt
    11521 gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct gctgaccggt
    11581 gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga cgactctgtg
    11641 ccactgttca atctgtacaa tccggtgaat cccgatttcg acttcaatgc caaagatccg
    11701 gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt ttatgttcag
    11761 gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga ctgggcagat
    11821 caagaatctc ttaaccgcgt tgctgggacg accaataaac gtgatgacaa acagtttacc
    11881 tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt cagctatagc
    11941 gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc accgtctaaa
    12001 ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat tgtagttact
    12061 ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga gggttccttc
    12121 ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg
    12181 ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga atacaccacc
    12241 gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc ttcgctgtgg
    12301 gctgactata ccttctttga cggtccgctt tcaggtctga cgctgggcac cggtggtcgt
    12361 tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag ttatacggtc
    12421 gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc caacgtggcg
    12481 ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc
    12541 tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt ctaatttctc
    12601 ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag gaatacacga
    12661 atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc
    12721 ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg attggtcaca
    12781 acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct ccgtcagaag
    12841 gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg tttgcccgca
    12901 aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg cgtgaactgg
    12961 tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg gcagatcgcg
    13021 aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat cggctggtcg
    13081 atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg gcgcaggata
    13141 gccgttgtct gttgctcgac gaaccgactt cagcgctgga tatcgcccac caggttgatg
    13201 tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt gccgtgttgc
    13261 acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc agtggtgaaa
    13321 tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa atgatttatg
    13381 gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt
    13441 gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg cgctttctcc
    13501 gttgttatgg cagatgaata ccgcccacgc ggcggctatt gaccccaatc gtattgtggc
    13561 gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt acggcgtggc
    13621 ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact cagtgattga
    13681 cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac cgtcgtttat
    13741 ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg cgccgggtcg
    13801 cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat cgctgacgga
    13861 aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc aatacgaaga
    13921 ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt tactgctgac
    13981 gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt tccaggaaat
    14041 tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct ggggcagtac
    14101 cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct gttttgatca
    14161 cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg ccatgccgtt
    14221 tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg caacgctttc
    14281 ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt gagtaaacga
    14341 attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac ggcgctcacc
    14401 tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc ctggtcgccg
    14461 gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg tctggcgatt
    14521 tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca agtactgcgt
    14581 aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact ggggattacc
    14641 gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc gctggcaggg
    14701 gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct gtcgccggta
    14761 acgctgattt tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa tcagttactg
    14821 gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg aacgctgacg
    14881 caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg tgtgatgctg
    14941 acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt ggcgcgcaat
    15001 ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat tgtcatcagt
    15061 gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc gccgctgctg
    15121 gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc gttgattggt
    15181 gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt gtggatggaa
    15241 gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg gctgctgccg
    15301 cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc gactgaacgc
    15361 caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc tgtggtggtg
    15421 gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc gttgctcgag
    15481 gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg
    15541 gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag cccggaagtg
    15601 ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct ggtaccgggt
    15661 aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgac gctgttgatc
    15721 attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact ggcggggatg
    15781 gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg tgacccgcga
    15841 atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga tgctcaggtc
    15901 tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg ccgccgctgg
    15961 ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc gctgacgccg
    16021 acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac gatgactatt
    16081 ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat gggctttcga
    16141 cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct ggtgttcgct
    16201 gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct gctgtcgacc
    16261 tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta atagaatgcc
    16321 tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt gcagcatcga
    16381 tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcctgct tcaaacgctg
    16441 acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta aaacgctcga
    16501 cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta aagaagatac
    16561 cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct gccgcttcca
    16621 gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg cccggctgcg
    16681 cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg gaaagcgtac
    16741 ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga cgaccaccga
    16801 atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa tctggcacta
    16861 cggcgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc acttcatcga
    16921 tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg aactctggca
    16981 gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag gcaatctctt
    17041 gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta caggttaagg
    17101 tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac gtgagtgcgc
    17161 cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa
    17221 tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc gtcgcctcgg
    17281 tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc accagttgcg
    17341 ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg gcggcttcaa
    17401 tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc caggaaccga
    17461 cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc gctttttcga
    17521 taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc acaccgccag
    17581 ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag ggttcctgat
    17641 tcgtagaaaa agtgaatggc tgctattcca tgttattcat aacaagttaa atactcgtca
    17701 aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa gttttgtgca
    17761 tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc
    17821 ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc tttagaaaca
    17881 ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga gcgcgataaa
    17941 accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg gctggctgcg
    18001 gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc gctggtacat
    18061 actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc ggtgctggcg
    18121 atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc ggggatcccg
    18181 gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt attgccggtg
    18241 aaattctttg gcgggctggg gacactcggc ggaggcatgg ggctagggcg cgaaggacca
    18301 accgtgcaga tcggcggcaa tatcagtcgc atggtgcttg atattttccg cctgaaaggt
    18361 gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc tgcggccttt
    18421 aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca gtttcgctat
    18481 acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg
    18541 atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc gccgcttaat
    18601 acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc tatttttaat
    18661 aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa tattaccaaa
    18721 tgggtactga tggggggggc gattggcggt ctgtgtggat tgctggggtt tgtggcacca
    18781 gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa tttcagcatg
    18841 ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg cttctcttcc
    18901 ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct gggaaccgct
    18961 ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg gacgtttgct
    19021 attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac ggggatcatt
    19081 ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat taccggtctt
    19141 ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc gattcttgcg
    19201 cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc atcagccagc
    19261 gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt gggttagaat
    19321 ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc actgccgctg
    19381 gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga agataacccg
    19441 aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca gtatggtttc
    19501 acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg
    19561 gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta caccgaagga
    19621 ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt
    19681 tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg cacatcttct
    19741 ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc caacgaatag
    19801 ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt tccagtggta
    19861 cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag gcagttgcgt
    19921 agatttctgt acgtaaaatc atggggattt cgcggaccag tacatcacga atgatcccgc
    19981 cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct tccgcattaa
    20041 aggctttatt cacgctaatg ccgacaaaca ccgccagacc aacggcgtcc agcaccggca
    20101 acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc atgctggtga
    20161 ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg tgatccagcg
    20221 ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc agaacaccaa
    20281 aagggtccat acgcaatttt ccggctaaca aaacgccgta gatggcaaat accgctgtgc
    20341 cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc tgaaaaggtc
    20401 aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag gataatacgt
    20461 gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt gagctgttca
    20521 ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac aatagcctgt
    20581 ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg gctgtcttta
    20641 aagatgtttt ctccgccaca aacttcgagt acctggttct gaatcgactc ttttccactg
    20701 gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt atcagcatat
    20761 tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc ggccttgtct
    20821 ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc aatgcttgtc
    20881 gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg ccgctcggca
    20941 ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg ttccagattc
    21001 atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata gtcggaatag
    21061 ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa
    21121 agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga cagggcgacc
    21181 agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact caaccatcag
    21241 gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag actgttgatc
    21301 ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt ggcagacatg
    21361 ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt ggcggatttt
    21421 cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc
    21481 gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat cgtcagcttt
    21541 aaagcctgcc ggacagcctg gtaactgacc gtattcataa ccaaatgccg tgacatccgc
    21601 gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg ttggtgccag
    21661 gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca acagcagagt
    21721 ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa cctcggttcc
    21781 attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac ggttttcgat
    21841 tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga ttttcataga
    21901 tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg gggaagcgta
    21961 tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata aactggcatc
    22021 gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga
    22081 gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa aagacccagg
    22141 tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg atggaagtcc
    22201 agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag cttaaattgc
    22261 tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt gagatgtcat
    22321 gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca gcgataaatg
    22381 actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg actgacgatc
    22441 gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac gagctgcggc
    22501 gcaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt cgtctggtgc
    22561 atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta aaatataccc
    22621 gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg aaaaagccgg
    22681 gttattatct ttctgaagaa gcctatattg cccgcttgcg taaagaactt aatttggcgc
    22741 tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc tcctattgtg
    22801 tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag ctttatcatc
    22861 atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg gtggttgaaa
    22921 atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat cagtttttta
    22981 tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa cgatttattg
    23041 ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa gatgccagcg
    23101 aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg tttagccatc
    23161 cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta ttagagattt
    23221 atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa aaagaacggg
    23281 tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc catcggctgg
    23341 cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg ctatgggaat
    23401 attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac ctctatgcgt
    23461 gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt aaagacgaac
    23521 aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata aaacgaatct
    23581 gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg aaaaaaaaca
    23641 cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg ctctctgcaa
    23701 cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt gcaccgatgc
    23761 tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca accgttaata
    23821 cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg ttctgccagg
    23881 aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt aatggcggcg
    23941 gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc gataaaggct
    24001 atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc cagctgagcg
    24061 atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat atcgcgctga
    24121 tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct gatgcactgc
    24181 gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag acggtaactt
    24241 ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac gaaaacttca
    24301 tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt aacctgaacg
    24361 gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac atcggtatcg
    24421 gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg gaatacggcc
    24481 aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa ctggcgaaag
    24541 cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct aattcctccg
    24601 ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt aagccgatca
    24661 gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc
    24721 tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag cagagcagcc
    24781 agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag atgagcaaca
    24841 aaggcaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg gctgcgcaga
    24901 tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg aaaaacatcg
    24961 ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac attcagcgcg
    25021 gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc tgaatacggg
    25081 aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt catcatcctt
    25141 tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt gcataacgga
    25201 ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc
    25261 atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat tatcggcagc
    25321 ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact ttcacaggga
    25381 cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg gcaggggatt
    25441 aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca
    25501 gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat gctggaacag
    25561 tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact ggtgatgaac
    25621 ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg ctggggatcg
    25681 atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag cttggcgtgg
    25741 acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc gagcgtaata
    25801 atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt
    25861 ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt acccgcatga
    25921 aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc ggtcctggca
    25981 gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc
    26041 cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc gacagtttgc
    26101 gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa gcgatggaca
    26161 ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg caaccgctgg
    26221 caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt aatcgtatat
    26281 cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt
    26341 tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcggcgct ggctcattcc
    26401 cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg attatttacg
    26461 cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga ctgactccag
    26521 gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt cacgctgaca
    26581 gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct ggacttcttt
    26641 ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat atttaaactt
    26701 aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca gaatatcgtt
    26761 gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa tacccagact
    26821 tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg cttattttag
    26881 catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc ttccagactt
    26941 ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt ccatgggatg
    27001 gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa gatgaaaaag
    27061 tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca gaaagcatac
    27121 gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc agttcgttaa
    27181 tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca cagtagaggc
    27241 tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc gccggaacgc
    27301 gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga ccaatgtata
    27361 cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact tcagagcgtg
    27421 cgccaatgaa gcagttatct tcaataatgg tcgggttagc ctgcagcggc tccagcacgc
    27481 cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca caagaaccga
    27541 cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg ttaacgtaag
    27601 acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc gctggtggca
    27661 caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat ttcatcggca
    27721 ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta atacggaaag
    27781 agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca attttttccg
    27841 ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct tcgcgggtaa
    27901 cggtgtctgc attggctggc gtgatctcgg cacggcgttc aaaagcggtt tcaataatgt
    27961 tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac cctttatcgt
    28021 ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg ttattaagcg
    28081 cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca atggttgtaa
    28141 ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact cgcgccagca
    28201 gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta tgggtcggca
    28261 aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg cgacggggct
    28321 gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga ataacctcat
    28381 ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta tccatcgcca
    28441 taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg cggtctaatt
    28501 cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa atctcggtgc
    28561 ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca tgctgtaata
    28621 aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag ttagcacgac
    28681 agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc agtagtgcca
    28741 gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt tgcattccgc
    28801 gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc ttccagctat
    28861 tccacagtgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc agatagcgca
    28921 gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg tcctgaatat
    28981 cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag gcaaccagct
    29041 gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc tgagcaccga
    29101 gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg gcgatgaaaa
    29161 tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg cgctggcgcg
    29221 tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt tcatccaccg
    29281 tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat tgcggcatat
    29341 acgcgccgag cacgctatgg cgatgcattg gcaatagtcc gcgccgcacc gctccggggt
    29401 gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc ggttgttgca
    29461 gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg gtgatcgcac
    29521 tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc ataaatagtg
    29581 tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg agtggacgtg
    29641 gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc agcatctggt
    29701 tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc tcgaccggtt
    29761 cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga tcgaataaca
    29821 gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc cacagtatat
    29881 gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac ccgaccattt
    29941 catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg tgaatatcgc
    30001 gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg ccatggtaac
    30061 gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg gaaggccaga
    30121 atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg ccaattaata
    30181 agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc aacatgcact
    30241 cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc agcgttaaca
    30301 gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa atcagtaaat
    30361 cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca accaatgcca
    30421 ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc tggtctatga
    30481 actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag gcatcaccca
    30541 gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat tcatcacggg
    30601 gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg tttgcgtact
    30661 gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca ttaaaaaagc
    30721 cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat ggtgtcatcc
    30781 ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg ctcatattgt
    30841 gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt gcggatctct
    30901 tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt cagcacgacg
    30961 ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc gcgaccaata
    31021 ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa tttctgaatc
    31081 gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc caggtacagg
    31141 ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc gacgataaac
    31201 attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat gttaacgata
    31261 tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac ttcattaata
    31321 gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac cgcgtgttgt
    31381 tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac gcccggttta
    31441 acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac gcgcattttt
    31501 tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca gcgtcggtga
    31561 taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa ttgagaatca
    31621 ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt cgacgtctga
    31681 tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta taaagcgcgc
    31741 cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct cactttgtgt
    31801 aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt aatatgggat
    31861 acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac tgtttccatg
    31921 cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg gaacccgaaa
    31981 atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct tgagaaaact
    32041 gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg caaaggtaaa
    32101 atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc tgctctgagc
    32161 tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa ctggaaaacc
    32221 gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga cggtactttc
    32281 gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa actggaaaac
    32341 agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt aatcgatgct
    32401 gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt atttgctatc
    32461 gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa cgacgacgca
    32521 atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga aggccgttct
    32581 caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata aggcttgata
    32641 actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc ccgggagctt
    32701 acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg cgaaatatga
    32761 agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct gcatatggcc
    32821 ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc tccgaggatt
    32881 ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt actggcgcag
    32941 gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag ctggcaatcg
    33001 aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac gttgctgctg
    33061 acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa gttaactgcc
    33121 agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa gttctggacg
    33181 cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc gaagaagaac
    33241 gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt gctgcgctgg
    33301 aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg gttgctgcta
    33361 aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca agcaagccag
    33421 aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac caggtacagc
    33481 tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg gttgaaggcc
    33541 gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt atggaaccaa
    33601 gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc ttcatccgtt
    33661 tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa gttgctgcga
    33721 tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct ttttgtgccc
    33781 atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta caatgtcgct
    33841 agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta ccaatgcaaa
    33901 acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg gcactgaagg
    33961 cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag aactggttga
    34021 actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg gcgctggtct
    34081 ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg cgaccgtaat
    34141 gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc gtctgatgtc
    34201 cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta tcagcctgtt
    34261 gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct ttaccaccga
    34321 ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga aagcaaccaa
    34381 agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca tgtacgagca
    34441 actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg cggccttcac
    34501 gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac cgggtgcgct
    34561 gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat tcccgtgatg
    34621 gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg ttataaggcg
    34681 ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt acaaaagtac
    34741 gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct aataatccag
    34801 acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac tgtgtgtgac
    34861 tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg atatcagaaa
    34921 agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa tcagcaaaat
    34981 acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat attacggcac
    35041 gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta ctctgaaaat
    35101 caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg cctccgatct
    35161 cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc cgctgacgga
    35221 agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag cgcgtgttgc
    35281 agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga aagataaaga
    35341 gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga ctgatgctgc
    35401 aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc agttctgatt
    35461 tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg gcgttttgct
    35521 ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg ggctcgaccg
    35581 gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac ttccgcgtag
    35641 ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg gaattctctc
    35701 cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg atgctacagc
    35761 aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat atggcagcgc
    35821 ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg ttacctacgc
    35881 ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca ctggttacct
    35941 gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta ccggtcgata
    36001 gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat ctgggatacg
    36061 ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt ggccctttcc
    36121 gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc cgtcatccga
    36181 actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac aaaggtctgg
    36241 aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa gtgctgattc
    36301 acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt ctggcgcagc
    36361 tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg aatcgcgtga
    36421 actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt gccgcaccgg
    36481 attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa ggccaggcag
    36541 cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt gcgcaacaaa
    36601 tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg gatatgcgcg
    36661 aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa gtcgccagaa
    36721 aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg cgctatttgt
    36781 tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt ggctttataa
    36841 ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt attcctgagt
    36901 accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc aaccacttag
    36961 cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg gcaatggtcg
    37021 ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg caaaatccgt
    37081 ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc tgtatgcctt
    37141 tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg aactgtttgt
    37201 gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc tgcgtattat
    37261 tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat ctgaagcgct
    37321 aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg gacgttggga
    37381 tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc tgcaaccaga
    37441 tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg cccctgtaga
    37501 tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt ggcaaattgc
    37561 ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac aagactttga
    37621 aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg agcccggtga
    37681 tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt
    37741 aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg ccattgtaac
    37801 gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt ttaccactcg
    37861 ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga tgctttttct
    37921 gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac tttgggcttc
    37981 gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt ccgcagcaat
    38041 ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg ttcccttctt
    38101 ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca gtggcgcaat
    38161 atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat atatgtttgg
    38221 caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa cctggcaagg
    38281 ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca tgtgggcgaa
    38341 tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt tagcctcagt
    38401 gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg acagcggtca
    38461 tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg ctgcggtacc
    38521 ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg taatatgctg
    38581 agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat caccgtgcat
    38641 gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata
    38701 gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata tgttatcgcc
    38761 ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg
    38821 gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc gattattgcc
    38881 gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt ttttattatt
    38941 ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat agctgcggaa
    39001 gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg
    39061 gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac cattacagta
    39121 gcgccatttg acagcgacca acggcgggat gtaaagctcg atttacgtca ctgggcgttt
    39181 gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg gccgcaaatt
    39241 gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca
    39301 ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac ctttgtgatg
    39361 cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca ggggagtccc
    39421 ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc
    39481 ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg ccagtatggg
    39541 ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa gctgacggtc
    39601 agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag tgggccgatc
    39661 tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta cctgccgttt
    39721 cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc cgtacttgac
    39781 ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt atccgagcgg
    39841 gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat ggggcttgca
    39901 cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata ataacgatgg
    39961 cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg
    40021 aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg
    40081 ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat atcagtaata
    40141 ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg
    40201 atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact ttctccggta
    40261 acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt gtgcgtgtgg
    40321 gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa gacttctact
    40381 acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg ccgcgcaacc
    40441 gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag caaattaaca
    40501 ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa ctgcgtgacg
    40561 aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg
    40621 atcttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc aacatcgact
    40681 ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg aacatcaccg
    40741 aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc gggcactccg
    40801 ctgaaattga gcaactgact aagatcgagc cgggcgagct gtataacggc actaaagtga
    40861 ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc tatccgcgcg
    40921 tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc gtgaacgttg
    40981 atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat acctcgaaag
    41041 atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg agcgatctgg
    41101 tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc gataccgata
    41161 cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg aaagaacgta
    41221 acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg agcttccagg
    41281 ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc aacgggacca
    41341 aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc accgtagatg
    41401 gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac gccgacctgt
    41461 ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg attaacgaat
    41521 ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac atgcagcctc
    41581 aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc tctgatcagg
    41641 ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat aacaagcttg
    41701 accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa gtgaccattc
    41761 ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat gtgccgatcg
    41821 atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt gatggtttag
    41881 gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc accgtgcgtg
    41941 gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag gccagtaatt
    42001 atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac ctgtgtaaat
    42061 cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc atcaccccga
    42121 cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc tgggatatgg
    42181 gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg gactatagtg
    42241 atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc ccattggggc
    42301 cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag gcagaacagt
    42361 tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt agtggtagtg
    42421 tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa ctgcaccctc
    42481 cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc tgcaggtctt
    42541 ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt caatatgggc
    42601 agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga aaatgagttc
    42661 aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaaa
    42721 ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt gatggctcag
    42781 cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg tcgttccaac
    42841 gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt tgccaacagc
    42901 caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa
    42961 gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat tcgactggct
    43021 gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt catcaccggc
    43081 gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa cccaaaatac
    43141 cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga cgatcttcct
    43201 ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc gcgcatggcg
    43261 caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc ggtgatcgac
    43321 gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat tgagtccggc
    43381 gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa aaacagcaaa
    43441 atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat ccagatcggt
    43501 cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg ttatgccaac
    43561 gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg cgatcgcgtg
    43621 gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt tattggcaat
    43681 ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg cgacaatacg
    43741 gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc
    43801 ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac ggttacgggc
    43861 atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg cattccgctg
    43921 caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga tgacatgagc
    43981 aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc catcttttgt
    44041 tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt tattattgtc
    44101 gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact ctgcagattg
    44161 aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg
    44221 attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct
    44281 tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg gaagcaatgg
    44341 cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt
    44401 actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct ggcgatcaaa
    44461 tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg
    44521 ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt agccgggagg
    44581 cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg gaagagggcg
    44641 cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc catgtcgaaa
    44701 ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc
    44761 gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat ttgaaatatg
    44821 ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca
    44881 ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac aacttactga
    44941 tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt ctcgccaaca
    45001 acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc ggcatgaccg
    45061 cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc tccggtgtgg
    45121 cgcaggacgt ccctccttat gttattgcgc agggtaacca cgcaacgccg ttcggtgtca
    45181 atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct atccgcaatg
    45241 cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg gaaattgctg
    45301 aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca cgctcaacgc
    45361 gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg tcgccggaga
    45421 aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac gtgtgcccaa
    45481 cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg aagcctggta
    45541 cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt
    45601 actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt
    45661 tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa agcagggtat
    45721 caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac gcgttttcaa
    45781 aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag cgttttatga
    45841 caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca tgccgttaga
    45901 tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc actgtctggc
    45961 attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt tcttgaaaac
    46021 tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc tggtgaatgc
    46081 caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc tgtcggttca
    46141 tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac tactggcgtc
    46201 ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg gatatcgcat
    46261 gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg tctcgctgcc
    46321 aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt gtgagccgca
    46381 aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc acgcgatgca
    46441 cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc aggcggcaca
    46501 agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca gctggttgcg
    46561 ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc
    46621 cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag cgaaaaacgc
    46681 cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct gggccgcgcg
    46741 gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc gatgcagcgt
    46801 gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg
    46861 aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc agaaatcagt
    46921 gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct ggatattgtt
    46981 ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca tctggaaaaa
    47041 ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt caaacgcgca
    47101 ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct gaagatgtct
    47161 gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga tggcttggcc
    47221 aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact ggcgatcacc
    47281 gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg cgcagggatt
    47341 aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga tgagttaacc
    47401 cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt gctgatctca
    47461 aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga ctggcttatc
    47521 gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt cggacgcagt
    47581 cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga agaacacttc
    47641 ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga aagctatctg
    47701 cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac taacgatgtg
    47761 cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat ccacgacggc
    47821 tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata tatgcgtagc
    47881 gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa caccgttgag
    47941 atcgccaaac gctgtaacgt aactgtgcgt cttggtgaat acttcctgcc gcagttcccg
    48001 accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg cctggaagag
    48061 cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc ggaatatgac
    48121 gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg ctacttcctc
    48181 atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg gccaggccgt
    48241 ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct cgatccgctg
    48301 gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat gcctgacttc
    48361 gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc ggacatgtac
    48421 ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa agcggtgatc
    48481 cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat ctcgaaactg
    48541 atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc gcagctgccg
    48601 gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg caaactggaa
    48661 ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc gaccaaaatt
    48721 accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac ccagtttgat
    48781 aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt gcgtacgctc
    48841 accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa tggcgagcca
    48901 ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat gctgcaacgc
    48961 tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct gatcaagcgt
    49021 ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg ccctggtccg
    49081 ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga agagatctcc
    49141 tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc aacctacggc
    49201 attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg ttataccctc
    49261 ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga gatggctaag
    49321 cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga actggcgatg
    49381 aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc gcactctgcg
    49441 gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc ggcggagttt
    49501 atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg cctggtggat
    49561 gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg tctttaccat
    49621 ttccacgtca acgacgatgg cgaaatcgtg tatggtattg gcgccatcaa aggggtaggt
    49681 gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt ccgcgaactg
    49741 tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct ggaaaaactg
    49801 atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat gaactcgctg
    49861 ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg tcaggccgat
    49921 atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc cagctgccaa
    49981 ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct gtacctgacc
    50041 ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg cgtaaggctg
    50101 aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct cgttgttgcc
    50161 gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct ggatgaccgt
    50221 tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca gcaattgctg
    50281 gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt cagcggtggg
    50341 cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa atatgctcgc
    50401 gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa ccgactccgt
    50461 cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta tcagagggcg
    50521 gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag cgatcgttta
    50581 ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt tgactaatac
    50641 aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga gctggaagcg
    50701 aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat taacatcgat
    50761 gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat cttcgccgat
    50821 ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta taccctggat
    50881 tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc gtatgcagac
    50941 gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat gatcattggt
    51001 catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat gccagcgcca
    51061 gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa gatgcctatc
    51121 atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga gcgtggtcag
    51181 tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc ggtagtttgt
    51241 acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg cgataaagtg
    51301 aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc gtccattctg
    51361 tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat cgctccgcgt
    51421 ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg cgctcaccgt
    51481 aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct ggccgatctc
    51541 gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat gagctacggt
    51601 tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt tttatcgcca
    51661 cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg aacatcattg
    51721 ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa ctggagtcgg
    51781 cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat ttgctgaaat
    51841 ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag tacagtctcg
    51901 atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc ttcatcaaca
    51961 cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg ttttttgaat
    52021 atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc aacgaatatc
    52081 ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa gagcggaagt
    52141 acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc ccggttggct
    52201 gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct atttcggtca
    52261 ccgagcttgg ttcgttgctt gaccacaccg ggccacacct ggaagcggaa gagtacatcg
    52321 cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaatatcg acgtcgaaca
    52381 aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac cgcaactgcc
    52441 ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg ctgaaaccga
    52501 cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact cgcgacagca
    52561 tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg gtgatcacca
    52621 actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg ctggatgtcc
    52681 cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca atctaccagg
    52741 gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa acgcaatcga
    52801 cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa ggcgagtatg
    52861 acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc agttatccca
    52921 ttgttgcttc ggttaagacg gcggcggcga tgctgcgtgg taatccgggc aaacggctga
    52981 ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg ctgcgggaag
    53041 agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa gccgaatgca
    53101 ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc gatcatatgt
    53161 ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag ggcaatatga
    53221 gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt gggatcgtag
    53281 tagagaaaac cggcccttat aacctgctgt ttctctttag tattggcatc gataaaacca
    53341 aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat ctcaacctgc
    53401 ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac cgcaatatgc
    53461 gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat ctttccggtt
    53521 tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat caggcatggc
    53581 agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc ggtagagtat
    53641 cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca ggagaaatgc
    53701 tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc gtagggcaac
    53761 attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac ggcgtttacc
    53821 gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg cgagtaacgt
    53881 gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc atattgcgat
    53941 tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg gtttcacgct
    54001 gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg cgcttaatgg
    54061 gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca gccgaccgga
    54121 agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg cagtggcgca
    54181 ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca cgcaaaaacg
    54241 cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc agtaaggctt
    54301 gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat cgcaccatca
    54361 tgacactcac gctcaataga caacttctca cctcacgcca gattctggtg gcctttagcg
    54421 gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg gaaaatccgg
    54481 gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc gatgcctggg
    54541 ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc gaacgcgtac
    54601 aacttgcgca agaggggctg gggattgagg cccaggcgcg gcaggcacgt tatcaggcat
    54661 ttgcccgtac cctattgccc ggcgaagtgc tggtcaccgc gcaacatctc gacgatcaat
    54721 gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt tcggctatgg
    54781 cggaagtttc ggagtttgct ggaacacggc ttatccgccc gttgcttgcc cgcacgcggg
    54841 gggaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac gaaagtaatc
    54901 aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta ttgcagcagc
    54961 gttggccgca ttttgcggaa gcaacggccc gcagcgccac actttgtgct gaacaagaga
    55021 gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg caggggacgc
    55081 tgcagattgc gccaatgctg gcgatgagtg atgcccgtcg cgcggcgatt attcgccgct
    55141 ggctggcagg gcagaatgca ccgatgcctt cccgcgacgc gctggtgcgc atctggcagg
    55201 aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg tttgggcgcc tttgaaatcc
    55261 gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagc gaaaccattg
    55321 tgctgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga accgtacagc
    55381 ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc gtgcgtttca
    55441 aagcgccagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg aagaaaatct
    55501 ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg ttttatggag
    55561 aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct gaaggtgaga
    55621 atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga taagacgcat
    55681 caaacgtcgc atccggcgaa agtcaatcag gattcgctta ccaccaccgt accgatttca
    55741 gggtggctaa agctggtaat tttatccaga cgcagctcgc gggtttcgcc agcggcctcg
    55801 acgaccagat actccacatt tttgcgggag actaaatcac tggcttttgc ctgcaatttt
    55861 tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag ctcaagatta
    55921 tcgtaatcat cacaattaat tggttgatac gtatcattca ttgacataat cgctcaccag
    55981 taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg cgtcgtcggc
    56041 agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt atcctaagtc
    56101 tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt ccacatgccc
    56161 tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag ataaactctg
    56221 tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa ctgcacgtat
    56281 tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa agcgacaatt
    56341 tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga tgcctctctg
    56401 acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt gctggcgtgg
    56461 ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga tgatgagccg
    56521 gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct ggggctgccg
    56581 gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct gtttacctat
    56641 aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt gcagtttgcc
    56701 aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc ctttgccgat
    56761 cgtaccatga cgctgcaggc aacgattcag gatggtgtta tctggttatc ggatgataag
    56821 aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg atttcccgac
    56881 atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag ggcgctggcg
    56941 ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt cgggcgtcca
    57001 gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg atcagcagtg
    57061 atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac cgcgaagccg
    57121 ctctggcccg gctggtggca gtgattaaag atttaacaaa agaacaaaaa gcccgacgac
    57181 ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca caaaaatcaa
    57241 gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa ggaatggatg
    57301 gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct gatgggatgt
    57361 aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact gaaaccgatg
    57421 ccgcaaagtt ggcgcggcgt gctaccgtgt gccgattgcg aaggaatcga aatctctctg
    57481 ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc tcgtgaagaa
    57541 ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct ggtattaacc
    57601 gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga gatgctcgat
    57661 cgtgaaggca atccaattga atcgcagttc aactatacgc tggaaccggc acaatccagt
    57721 ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga tgcggcgacc
    57781 ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga gctggagcgt
    57841 agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt agaaggtcac
    57901 tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc gcccgatacg
    57961 gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac ctgcttaagg
    58021 cagaaacaaa cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag catataatgc
    58081 aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg catccggcat
    58141 tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc gccttgatat
    58201 aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta aacgcagaga
    58261 catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat tgcgccagtg
    58321 taagtggatg cccggatttg gcaaatgcat cagtgacaaa ttccgaacaa aaccacgact
    58381 ttttgtctcc ttcgcacaca ctgcttaact gcgctttcgc caggtcgctg acgcactgtt
    58441 ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc ataaagggaa
    58501 taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg gcaaacgcgg
    58561 tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa agcttatcac
    58621 tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc gcttctgcaa
    58681 cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg aagacgcgga
    58741 ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt ttgagatcag
    58801 gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg aatttgacag
    58861 cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg ctgatatcaa
    58921 cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac gcctttggtt
    58981 tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc tttctggcat
    59041 cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat taactgtttc
    59101 tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg gtcgcccagc
    59161 acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc cggacgctct
    59221 ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta cagtttctca
    59281 gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc cacctggaac
    59341 ggcgcgatag cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc aatcgccgca
    59401 gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg gttacggcca
    59461 tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag ctggaagatg
    59521 tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg gcttggatcg
    59581 ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg atcccagttg
    59641 ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact catcgccgca
    59701 acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga acccggaccg
    59761 gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc
    59821 ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc
    59881 agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt tttctcaatc
    59941 ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt atcaaccagc
    60001 gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc tgccagttca
    60061 atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc
    60121 agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc ctgtacggcg
    60181 cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag
    60241 gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat caggaattcg
    60301 cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt ctggatctga
    60361 tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag gtcagtgata
    60421 acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc
    60481 agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc tggctgaacc
    60541 accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac gatgttttcg
    60601 acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga ggccagcttg
    60661 cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc ggcaggtgtc
    60721 tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt ccagttggaa
    60781 ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta ccagtgcaaa
    60841 agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag acttcaaaac
    60901 ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca taggttttgc
    60961 ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact tcgcggataa
    61021 acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg acctctgcgg
    61081 taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca ctggcatcgg
    61141 gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta ccatcgacca
    61201 gatccagact gccgagcttc agaatcacgc tgcctttatg gcaaacaacc tctttcagct
    61261 ctaccagcga catgccaatt gggttagggc ggaaagtaga gcgtgttgcg aaaaccccca
    61321 ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg ccttccatcg
    61381 tttgatgaaa gacgaaaagg atccataaat ggctgaatgc ttccaggccg cgaacggcgt
    61441 cggcctggtt gtaggaagct atgagatgca gttcgccgtt ggcgcttttt accagacctg
    61501 gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct atttgctcga
    61561 actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata cagcctgacg
    61621 atagcagccc ggcgtaccgc tggtgacttc gcagctatgc agtaatacag cattggcttt
    61681 cattttagag gcattgattt gcatctgctt acgtgcggtt ggaatgctcg gcggagagtc
    61741 ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga acggtttgcc
    61801 gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg ctttcggttt
    61861 tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag gggatctgct
    61921 taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag cacgcatagt
    61981 atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa aaatgacaag
    62041 acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta aaaattacca
    62101 gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt catcagactg
    62161 ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac gcgttacgat
    62221 caggtttacg tatggggagt ctttatcttc gacaaagata ccgtctttcg ccggagtcag
    62281 gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg cgtcgtccag
    62341 agaacgcggc agttgcggtg cttccagttc aacaattttc agattttttg ggttctcaac
    62401 aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca agcccacttt
    62461 ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg caacctgcga
    62521 accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa tcggataaac
    62581 aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct gatcaaggta
    62641 cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt cgtttggcag
    62701 aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt tcgcaacttt
    62761 ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt taatgtggtt
    62821 tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca gggctcccac
    62881 tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt atttatgttg
    62941 tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcaa actgaattaa
    63001 ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt tgtagccgat
    63061 gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac cacccatcgc
    63121 ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac ccggcaacgc
    63181 ttccggtaac agcactttac agacgatctg cattggcgtg gcacccattg cgcgggaagc
    63241 ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg caataaacgg
    63301 tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg taccgacaat
    63361 aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg aacggaaaat
    63421 gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt gccccggacg
    63481 cgtgacataa agcagaacgc caaccggcag accaatcaca aacccaaaaa atccggatac
    63541 gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca tcggctcaga
    63601 cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg gcggcttgcg
    63661 tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg ccaccggcgt
    63721 agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg gtttcagaaa
    63781 gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc acgcagtcag
    63841 taaatggctc cgtttgcaga cgttcctggt aatcttccgg gatatccaga tgcagggtcg
    63901 actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca cttaccgtgt
    63961 cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata cgcttcacaa
    64021 cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta atgtctttca
    64081 gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct tcatcacaca
    64141 gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt ttctgcccac
    64201 cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat gacagcaatt
    64261 ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc ggcagagcca
    64321 cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc ataccaatct
    64381 ggcggcgagc tttggtcaac tcggattctg acagcgtggt caattcctgg ccatcgacca
    64441 gcacgctacc ctcggttggg cgctccagca agtttacaca acgtataagc gtactcttac
    64501 ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc aggctgacgt
    64561 tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata ttcgaaagtt
    64621 ttatcattga ttatttatta tcgtcattaa gttagtcgta gcatctcgaa tgcctgaaac
    64681 gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc aatcaggttt
    64741 atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca tgccttatta
    64801 aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt gatggcacca
    64861 ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc gacggtgtta
    64921 ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta accaaccagt
    64981 ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc gagtggatgg
    65041 actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc ccgcatcatc
    65101 cgcaggggag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca catccgggga
    65161 tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat atggtgggcg
    65221 ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa gtgctggtgc
    65281 gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg ttaaatagcc
    65341 tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga ttaaaagatg
    65401 aacggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc cctataatgc
    65461 gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag
    65521 aaaaaatcct gaaattcagg gttgactctg aaataggaaa gtgtaatata cgccacctcg
    65581 cgacagtgag ctgaaagctg cgtcgcaact gctctttaac aatttatcag acaatctgtg
    65641 tgggcactcg aagatacgga ttcttaacgt cgcaagacga
//
LOCUS       DAJVPT010000008        65634 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVPT010000008 DAJVPT010000000
VERSION     DAJVPT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..306)
                     /locus_tag="OQM47_000510"
     CDS             complement(<1..306)
                     /locus_tag="OQM47_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1478791.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     "
     gene            complement(388..474)
                     /locus_tag="OQM47_000511"
     CDS             complement(388..474)
                     /locus_tag="OQM47_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1478792.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(486..977)
                     /gene="ilvN"
                     /locus_tag="OQM47_000512"
     CDS             complement(486..977)
                     /gene="ilvN"
                     /locus_tag="OQM47_000512"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1478793.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(980..2704)
                     /gene="ilvI"
                     /locus_tag="OQM47_000513"
     CDS             complement(980..2704)
                     /gene="ilvI"
                     /locus_tag="OQM47_000513"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1478794.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            2823..2867
                     /locus_tag="OQM47_000514"
     CDS             2823..2867
                     /locus_tag="OQM47_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478795.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3022..3966)
                     /gene="leuO"
                     /locus_tag="OQM47_000515"
     CDS             complement(3022..3966)
                     /gene="leuO"
                     /locus_tag="OQM47_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1478796.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            4625..4711
                     /gene="leuL"
                     /locus_tag="OQM47_000516"
     CDS             4625..4711
                     /gene="leuL"
                     /locus_tag="OQM47_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1478797.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            4804..6375
                     /gene="leuA"
                     /locus_tag="OQM47_000517"
     CDS             4804..6375
                     /gene="leuA"
                     /locus_tag="OQM47_000517"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1478798.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6375..7466
                     /gene="leuB"
                     /locus_tag="OQM47_000518"
     CDS             6375..7466
                     /gene="leuB"
                     /locus_tag="OQM47_000518"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1478799.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            7469..8869
                     /gene="leuC"
                     /locus_tag="OQM47_000519"
     CDS             7469..8869
                     /gene="leuC"
                     /locus_tag="OQM47_000519"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1478800.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            8880..9485
                     /gene="leuD"
                     /locus_tag="OQM47_000520"
     CDS             8880..9485
                     /gene="leuD"
                     /locus_tag="OQM47_000520"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1478801.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(9534..10712)
                     /gene="setA"
                     /locus_tag="OQM47_000521"
     CDS             complement(9534..10712)
                     /gene="setA"
                     /locus_tag="OQM47_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1478802.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(10814..10945)
                     /gene="sgrT"
                     /locus_tag="OQM47_000522"
     CDS             complement(10814..10945)
                     /gene="sgrT"
                     /locus_tag="OQM47_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1478803.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11034..12689
                     /gene="sgrR"
                     /locus_tag="OQM47_000523"
     CDS             11034..12689
                     /gene="sgrR"
                     /locus_tag="OQM47_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1478804.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      12723..12822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12853..13836
                     /gene="thiB"
                     /locus_tag="OQM47_000524"
     CDS             12853..13836
                     /gene="thiB"
                     /locus_tag="OQM47_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1478805.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            13812..15422
                     /gene="thiP"
                     /locus_tag="OQM47_000525"
     CDS             13812..15422
                     /gene="thiP"
                     /locus_tag="OQM47_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1478806.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15406..16104
                     /gene="thiQ"
                     /locus_tag="OQM47_000526"
     CDS             15406..16104
                     /gene="thiQ"
                     /locus_tag="OQM47_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1478807.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16218..16982)
                     /locus_tag="OQM47_000527"
     CDS             complement(16218..16982)
                     /locus_tag="OQM47_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1478808.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17068..17946)
                     /gene="araC"
                     /locus_tag="OQM47_000528"
     CDS             complement(17068..17946)
                     /gene="araC"
                     /locus_tag="OQM47_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1478809.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18285..19985
                     /gene="araB"
                     /locus_tag="OQM47_000529"
     CDS             18285..19985
                     /gene="araB"
                     /locus_tag="OQM47_000529"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1478810.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            19996..21498
                     /gene="araA"
                     /locus_tag="OQM47_000530"
     CDS             19996..21498
                     /gene="araA"
                     /locus_tag="OQM47_000530"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1478811.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            21698..22393
                     /gene="araD"
                     /locus_tag="OQM47_000531"
     CDS             21698..22393
                     /gene="araD"
                     /locus_tag="OQM47_000531"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1478812.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            22468..24819
                     /gene="polB"
                     /locus_tag="OQM47_000532"
     CDS             22468..24819
                     /gene="polB"
                     /locus_tag="OQM47_000532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1478813.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            24984..27890
                     /gene="rapA"
                     /locus_tag="OQM47_000533"
     CDS             24984..27890
                     /gene="rapA"
                     /locus_tag="OQM47_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1478814.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            27902..28561
                     /gene="rluA"
                     /locus_tag="OQM47_000534"
     CDS             27902..28561
                     /gene="rluA"
                     /locus_tag="OQM47_000534"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1478815.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(28678..29493)
                     /gene="djlA"
                     /locus_tag="OQM47_000535"
     CDS             complement(28678..29493)
                     /gene="djlA"
                     /locus_tag="OQM47_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1478816.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            29748..32102
                     /gene="lptD"
                     /locus_tag="OQM47_000536"
     CDS             29748..32102
                     /gene="lptD"
                     /locus_tag="OQM47_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1478817.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32155..33441
                     /gene="surA"
                     /locus_tag="OQM47_000537"
     CDS             32155..33441
                     /gene="surA"
                     /locus_tag="OQM47_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1478818.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            33441..34430
                     /gene="pdxA"
                     /locus_tag="OQM47_000538"
     CDS             33441..34430
                     /gene="pdxA"
                     /locus_tag="OQM47_000538"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1478819.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34427..35248
                     /gene="rsmA"
                     /locus_tag="OQM47_000539"
     CDS             34427..35248
                     /gene="rsmA"
                     /locus_tag="OQM47_000539"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1478820.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35251..35628
                     /gene="apaG"
                     /locus_tag="OQM47_000540"
     CDS             35251..35628
                     /gene="apaG"
                     /locus_tag="OQM47_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1478821.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            35635..36477
                     /gene="apaH"
                     /locus_tag="OQM47_000541"
     CDS             35635..36477
                     /gene="apaH"
                     /locus_tag="OQM47_000541"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1478822.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(36555..37034)
                     /gene="folA"
                     /locus_tag="OQM47_000542"
     CDS             complement(36555..37034)
                     /gene="folA"
                     /locus_tag="OQM47_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1478823.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37225..39087)
                     /gene="kefC"
                     /locus_tag="OQM47_000543"
     CDS             complement(37225..39087)
                     /gene="kefC"
                     /locus_tag="OQM47_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1478824.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39080..39610)
                     /gene="kefF"
                     /locus_tag="OQM47_000544"
     CDS             complement(39080..39610)
                     /gene="kefF"
                     /locus_tag="OQM47_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1478825.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(39658..41052)
                     /locus_tag="OQM47_000545"
     CDS             complement(39658..41052)
                     /locus_tag="OQM47_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1478826.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41111..41398)
                     /gene="fixX"
                     /locus_tag="OQM47_000546"
     CDS             complement(41111..41398)
                     /gene="fixX"
                     /locus_tag="OQM47_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1478827.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41395..42681)
                     /locus_tag="OQM47_000547"
     CDS             complement(41395..42681)
                     /locus_tag="OQM47_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1478828.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(42732..43673)
                     /locus_tag="OQM47_000548"
                     /pseudo
     CDS             complement(42732..43673)
                     /locus_tag="OQM47_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(43688..44458)
                     /locus_tag="OQM47_000549"
     CDS             complement(43688..44458)
                     /locus_tag="OQM47_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1478829.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            44814..46328
                     /gene="caiT"
                     /locus_tag="OQM47_000550"
     CDS             44814..46328
                     /gene="caiT"
                     /locus_tag="OQM47_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1478830.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46359..47501
                     /gene="caiA"
                     /locus_tag="OQM47_000551"
     CDS             46359..47501
                     /gene="caiA"
                     /locus_tag="OQM47_000551"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1478831.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            47630..48847
                     /gene="caiB"
                     /locus_tag="OQM47_000552"
     CDS             47630..48847
                     /gene="caiB"
                     /locus_tag="OQM47_000552"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1478832.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            48921..50474
                     /gene="caiC"
                     /locus_tag="OQM47_000553"
     CDS             48921..50474
                     /gene="caiC"
                     /locus_tag="OQM47_000553"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1478833.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            50583..51368
                     /gene="caiD"
                     /locus_tag="OQM47_000554"
     CDS             50583..51368
                     /gene="caiD"
                     /locus_tag="OQM47_000554"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1478834.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51374..51964
                     /gene="caiE"
                     /locus_tag="OQM47_000555"
     CDS             51374..51964
                     /gene="caiE"
                     /locus_tag="OQM47_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1478835.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52050..52445)
                     /gene="caiF"
                     /locus_tag="OQM47_000556"
     CDS             complement(52050..52445)
                     /gene="caiF"
                     /locus_tag="OQM47_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1478836.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(52707..55928)
                     /gene="carB"
                     /locus_tag="OQM47_000557"
     CDS             complement(52707..55928)
                     /gene="carB"
                     /locus_tag="OQM47_000557"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1478837.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIIEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(55946..57094)
                     /gene="carA"
                     /locus_tag="OQM47_000558"
     CDS             complement(55946..57094)
                     /gene="carA"
                     /locus_tag="OQM47_000558"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1478838.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(57550..58371)
                     /gene="dapB"
                     /locus_tag="OQM47_000559"
     CDS             complement(57550..58371)
                     /gene="dapB"
                     /locus_tag="OQM47_000559"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1478839.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(58538..59452)
                     /gene="rihC"
                     /locus_tag="OQM47_000560"
     CDS             complement(58538..59452)
                     /gene="rihC"
                     /locus_tag="OQM47_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1478840.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(59518..60468)
                     /gene="ispH"
                     /locus_tag="OQM47_000561"
                     /gene_synonym="lytB"
     CDS             complement(59518..60468)
                     /gene="ispH"
                     /locus_tag="OQM47_000561"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1478841.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(60470..60919)
                     /gene="fkpB"
                     /locus_tag="OQM47_000562"
     CDS             complement(60470..60919)
                     /gene="fkpB"
                     /locus_tag="OQM47_000562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1478842.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61006..61500)
                     /gene="lspA"
                     /locus_tag="OQM47_000563"
     CDS             complement(61006..61500)
                     /gene="lspA"
                     /locus_tag="OQM47_000563"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1478843.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(61500..64316)
                     /gene="ileS"
                     /locus_tag="OQM47_000564"
     CDS             complement(61500..64316)
                     /gene="ileS"
                     /locus_tag="OQM47_000564"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1478844.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(64359..65300)
                     /gene="ribF"
                     /locus_tag="OQM47_000565"
     CDS             complement(64359..65300)
                     /gene="ribF"
                     /locus_tag="OQM47_000565"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1478845.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(65308..65526)
                     /locus_tag="OQM47_000566"
     CDS             complement(65308..65526)
                     /locus_tag="OQM47_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1478846.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
BASE COUNT        15879 a        17887 c        16925 g        14943 t
ORIGIN      
        1 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgttcaag
       61 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
      121 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
      181 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
      241 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
      301 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
      361 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
      421 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
      481 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
      541 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
      601 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
      661 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
      721 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
      781 ttgtgtaatt gcttttcgat ctgctcaagt actttttcat cgcccacggt ctggatggtc
      841 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcct
      901 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
      961 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
     1021 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
     1081 acaccaggcg attattgcgc acctgttcca gcgcctcgct aagtttgctt tccagctcat
     1141 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
     1201 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
     1261 tcaccatccc cagatagcgg ttattgagat tcaccaccag tacgggcaac tcgtattgca
     1321 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
     1381 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
     1441 tgccgagacc gccggaattg atccagcgac gcggtttgtc gaatggataa taaagcgcag
     1501 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtccccatta gtcaaccgcc
     1561 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
     1621 actgaagagc gcgccactgt tcaatctgct gccaccagtc gcggatctca tccagcggtt
     1681 gatgggcgga ttcttgcgac aggagttcaa gcatttgttc gaggacctgg cgagcatccc
     1741 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
     1801 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
     1861 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
     1921 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccattccca
     1981 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
     2041 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
     2101 tttgcagagc acgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
     2161 tactgaccga ctcagggcag acatagggta atttgttcgc cggattaaga atatctttcg
     2221 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
     2281 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
     2341 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
     2401 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
     2461 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcacgcg
     2521 ccaggccatc ggccatatgc accgccgcct gctcatgacg aactaataca tgatcaatac
     2581 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
     2641 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
     2701 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
     2761 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
     2821 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
     2881 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa tagagagtcc
     2941 acacattcag aataaaattc tacattcaat aaaaaatgca gaataaacca gacattcatg
     3001 tctggcctat tctgcattca gttagcgttt gcaaattgag actaattgtt cttccatcca
     3061 ctgatggcct ttatcgcgcc cggcagcttc atgccaagag agataacagg ttctgctgtt
     3121 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
     3181 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
     3241 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
     3301 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
     3361 cagtaacggg cctttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
     3421 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
     3481 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
     3541 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
     3601 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
     3661 accaggcaat tcattttgta ctagttgcaa tgcctggcga actgaaccaa aaagttgaaa
     3721 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
     3781 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
     3841 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
     3901 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
     3961 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
     4021 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
     4081 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
     4141 catttaccac agcataataa tccataatgt tttgttgcga aaacaatcta atcataacta
     4201 ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc ttatttaata attaatttat
     4261 atatttaatg cattaattct taacattaat tgttcaataa tattcaccaa atcaatatca
     4321 aaaaaaatcg caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg
     4381 cattttgtcc cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac
     4441 cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgtagca
     4501 caattagcta attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg
     4561 gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga
     4621 tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg
     4681 cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc
     4741 gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa
     4801 accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta
     4861 caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt
     4921 gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa
     4981 accatcgccc gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa
     5041 gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt
     5101 attgctacct ccccaatgca catcgccacc aagctgcgca gcaccctaga cgaggtgatc
     5161 gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct
     5221 tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt
     5281 aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag
     5341 ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt
     5401 tccgtacata cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat
     5461 gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt
     5521 tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc
     5581 gccattaatc accaagagat atggcgcacc agccagttag ttagccagat ttgtaatatg
     5641 ccgatcccgg caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc
     5701 caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt
     5761 ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat
     5821 cgcatggatg agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct
     5881 ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc
     5941 ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag
     6001 tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa
     6061 gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc
     6121 actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat
     6181 gcgctgggtc aggtggatat tgtcaccaac tacaacggtc gccgcttcca cggcgtcggc
     6241 ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc
     6301 tggcgtgccg cagaagtcga aaaagagttg caacgcaaaa ctcaacacaa cgaaaacaac
     6361 aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccggga gacggtattg
     6421 gtccggaagt gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac cgctttgcga
     6481 tgcgcatcac caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac
     6541 cactgccgcc tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg
     6601 taggcggccc gaagtgggaa catttaccac cagaccagca accagaacgc ggcgcgctgc
     6661 tgccgctgcg taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg
     6721 ggctggaagc attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatccagt
     6781 gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg
     6841 gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg
     6901 cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag
     6961 ctaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat
     7021 acccggatgt cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag
     7081 atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg
     7141 agtgcgcaat gatcactggc tcgatgggca tgttgccttc cgccagcctg aacgagcaag
     7201 gttttggact gtatgaaccg gcgggcggct cggcaccaga tatcgcaggc aaaaacatcg
     7261 ccaacccgat tgcacaaatc ctgtcgctgg cactgctgct gcgttacagc ctggatgccg
     7321 atgatgcggc ttccgccatt gaacgcgcca ttaaccgtgc attagaagaa ggcattcgca
     7381 ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg
     7441 cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga
     7501 cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct
     7561 ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt
     7621 acgtcagccg ggcaaaacct tcgccactat ggatcacaac gtctctaccc agaccaaaga
     7681 cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa
     7741 agaatttggc gtcgagctgt atgacctgaa tcacccgtat caggggatcg tccacgtaat
     7801 ggggccggaa cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac
     7861 cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca
     7921 cgtactggca acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca
     7981 gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac
     8041 tggcagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt
     8101 aagcatggaa ggtcgtatga ccctgtgcaa tatggcgatc gaaatgggcg caaaagccgc
     8161 tctggttgct cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa
     8221 aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc
     8281 aactttcgat accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg
     8341 caccaatccc ggccaggtga tttctgtgaa cgacaatatt cccgatccgg cttcgtttgc
     8401 cgatccggtt gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg
     8461 tattccgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactctcg
     8521 cattgaagat ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt
     8581 gcaggcactg gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct
     8641 ggataaaata tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct
     8701 ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa
     8761 ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc
     8821 cgctgctgct gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca
     8881 tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg
     8941 ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg
     9001 cgcatctgtt taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact
     9061 tcgtgctgaa cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg
     9121 gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg
     9181 tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc
     9241 cggtgaaatt aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg
     9301 ggatccattt cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc
     9361 gctttaccat cgatgccttc cgccgccact gcatgatgaa tggtctggac agcatcggcc
     9421 tgaccctgca gcacgacgac gctattgcgt cttatgaagc aaaacaacct gcgtttatga
     9481 attagacccc ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg
     9541 tctttaacct ttgcggttaa aaataatgcg acaacagaaa taatcgcaat tacccagtag
     9601 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacaccagc cagaattacc
     9661 ccggtagaga tagtgttggt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
     9721 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
     9781 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccagtgta aaacagcact
     9841 cctgccacca ctgcaatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
     9901 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
     9961 ttgtctggca atcctaaatc gctactgatc cacaatggca tatcaataat gtacatagtg
    10021 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
    10081 tcaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacagaagc
    10141 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcact
    10201 gtaaagccgt aattcaacgc cagcataaag gccagcggtg gaccgattac ccatgccaga
    10261 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgcg
    10321 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
    10381 acaccacagg tgataagcgt cagataatga cgattaaatg caaacagtag cgcattgcca
    10441 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
    10501 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
    10561 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
    10621 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
    10681 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
    10741 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
    10801 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
    10861 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
    10921 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
    10981 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
    11041 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
    11101 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
    11161 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
    11221 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
    11281 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
    11341 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
    11401 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
    11461 aaacccatat tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg
    11521 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
    11581 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
    11641 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattacggac attgtgtcgc
    11701 cgacgccctg gacgctggat atccatctca cgcagccgga ccgctggttg ccgttactgc
    11761 tggggcaagt tccggcgatg atcctgccgt gcgaatggga aaccctcagt aactttgcca
    11821 gccatcctat tggcaccggt ccgtatgcgg tgattcgcaa cagcaccaat caactgaaaa
    11881 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgacgaagtt aacgtctggg
    11941 ttctgccgga aattgccgac gagccagccg gtggtctgat gctaaaaggt ccacagggcg
    12001 agaaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgacagcc
    12061 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
    12121 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
    12181 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
    12241 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
    12301 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
    12361 ggcatgaagg agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc
    12421 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
    12481 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
    12541 gccagcaact ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
    12601 agggacaacg cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat
    12661 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
    12721 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
    12781 atccggataa cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt
    12841 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
    12901 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
    12961 ggtccaaaga tcaaaaaagc ttttgaagcc aactgtaact gcgaactgaa actggtggcg
    13021 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
    13081 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
    13141 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
    13201 ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac
    13261 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
    13321 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
    13381 gatgacgccc cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc
    13441 tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc
    13501 acctctccgg cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt
    13561 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
    13621 gagctggcgc aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca
    13681 accggtaact ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg
    13741 accaaaccgg ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca
    13801 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
    13861 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
    13921 acgcgccgca gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc
    13981 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
    14041 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
    14101 caatgacctt gatcctcccg gtgctagttg ctgttttcgg cattcttagc gtctatggtc
    14161 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
    14221 acggcctgca aggcattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct
    14281 tattactcca ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg
    14341 ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc
    14401 cgccggttgc cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctatcgc
    14461 tgggcggcgg tccgcaggca accactatcg agctggctat ttatcaggcg ctgagttacg
    14521 actacgatcc tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc
    14581 tggtgctgtt gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg ctgctgcaag
    14641 gctggcgcga tccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
    14701 tggcgctgct gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
    14761 agttgccgga agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta
    14821 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
    14881 gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
    14941 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
    15001 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
    15061 tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta
    15121 tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg
    15181 ccctgaaacg tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt
    15241 ttggcgtagt ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc
    15301 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
    15361 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
    15421 gatatcacct ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
    15481 gagcaggtgg caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt
    15541 gccggttttc tgacgccagc cagcggttcg ctgactatcg atggtgttga tcacaccacg
    15601 acgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct gttcagccat
    15661 ctgacggtcg cgcagaacat cgggctgggg ctaaatccgg ggttgaaact gaacgcggca
    15721 cagcaggaga aaatgcacgc gattgcccgt cagatgggga ttgataattt aatggcgcgg
    15781 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg
    15841 cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt
    15901 caggagatgc tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg
    15961 gtgtcgcaca gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac
    16021 gggcgcatcg cctggcaggg taagactgaa gaattgttga gcggtaaggc gagtgcttcg
    16081 gcaatattgg ggattacggg ttagtatgca ctgtcagatg cggcgtgaac accttatccg
    16141 acctacggac agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc atgaggcaac
    16201 ccgcacaaga caatccccta aaccccaacc actttacgca aaatatcgat atacaccggc
    16261 atcaacgggt ggcgaattaa caccaccagc gccaccacgc cgatggcaga aatcaacggc
    16321 gtcagccaca acaaacggcc gcgcgacaaa taatgactta aacgatcggt tgctttaccg
    16381 ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc
    16441 agcaaccatt taaactcgcc gctctgcatt ccggctggaa tatcgatcgc cgcgcccgcc
    16501 agaatcccag gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgta
    16561 atgaattttg ccaccggcag atccagcatt cccgccacca ttggcaccag cggacgcgtc
    16621 gggccaacaa agcgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca
    16681 gttttatcga gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa cggcttttta
    16741 aaacgccagc ccagccagaa ggagatccag tcgcccagca gacaaccgac aatccctgcc
    16801 agccaagcgt gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc
    16861 agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac
    16921 accaccgcca tcaatgaata cacggtagat tgggtaataa agtgttccag caatgcttgc
    16981 ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa
    17041 gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt
    17101 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
    17161 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
    17221 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
    17281 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
    17341 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
    17401 agcctcgcgt acacgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
    17461 ccgcagtaac aattgctcaa gcagattcat cgccagcagc tccgaatagc gcccttcccc
    17521 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tacggctggt gcgcttcatc
    17581 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
    17641 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacaacc
    17701 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc
    17761 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
    17821 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
    17881 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
    17941 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
    18001 acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct
    18061 tattaaaagc attctgtaac aaagcaggac caaagccatg acaaaaacgc gtaacaaaag
    18121 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
    18181 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
    18241 ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg
    18301 gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag
    18361 agatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc
    18421 cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga
    18481 aaaccgtgct tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg
    18541 acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc
    18601 cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg
    18661 aagaagcgga agagattacc cgtttgtgcc acgcgccggg caacgttgac tactcccgct
    18721 atattggcgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc
    18781 aggacagcgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag
    18841 ctctgctttc cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc
    18901 ataaatctct gtggcacgaa agctggggcg gcttgccgcc agccagtttc tttgatgagc
    18961 tggacccgat cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg
    19021 atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg
    19081 tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc
    19141 ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac
    19201 agagcgttgg cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtgc
    19261 ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggtttg
    19321 gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc
    19381 aaatcaacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc
    19441 cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc acgccaaacg
    19501 ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac actccgctgc
    19561 tgttcggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta
    19621 ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc
    19681 aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg
    19741 accagtgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag
    19801 acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca
    19861 gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg
    19921 ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttgcgactc
    19981 tataaggaca cgataatggc gatttttgat aattatgaag tgtggtttgt cattggcagc
    20041 cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtt
    20101 aatgcgctga atacggaagc gaaactgccc tacaaactgg tgttgaaacc gctgggcacc
    20161 acgccggatg aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt
    20221 ctggtggtgt ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg
    20281 ctcaacaaac cgttgctgca attccacacc cagttcaacg cggcgctgcc gtgggacagt
    20341 atcgatatgg actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc
    20401 attggcgcgc gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca ggataaacaa
    20461 gcccatgagc gtatcggctc ctggatgcgt caggcggtct ctaaacagga tacccgtcat
    20521 ctgaaagtct gccgatttgg cgataacatg cgtgaagtgg cggtcaccga tggcgataaa
    20581 gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg ctatctggtg
    20641 caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc
    20701 tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa
    20761 gcggcgcgta ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc
    20821 accaccacct ttgaagattt gcacggtctg aaacagcttc caggtctgcc cgtacagcgt
    20881 ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt
    20941 cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac
    21001 acctatcact tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc
    21061 ccgtcgattg ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt
    21121 aaggacgatc ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc
    21181 ctgattgatc tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca
    21241 ccgcactccc tgccgaaact gccggtggcg aatgcactgt ggaaagcgca accggatctg
    21301 ccaactgctt ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat
    21361 gcgctgaacc tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg
    21421 attgataacg acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat
    21481 tacggatttc gtcgctaagt agccacatca ggtatgtaac gcctgatgcg acgctgacgc
    21541 gtcttatctg gcctacacgc tgcgattttg taggccggat aagcaaagcg catccggcat
    21601 tcaacgcctg atgcgacgct ggcgcgtctt atcaggccta cgcgctgcga ttttgtagac
    21661 cggataagcg aagcgcatcc ggcacgaagg agtcaacatg ttaaaagatc tcaaacgcca
    21721 ggtattagaa gccaacctgg cgctgccaaa acacaacctg gtcacgctca catggggcaa
    21781 cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta
    21841 cagcgtcatg accgctgacg atatggtcgt ggttagcatc gaaaccggtg aagtggttga
    21901 aggtacgaaa aagccctcct ccgacacgcc aactcaccgg ctgctctatc aggcattccc
    21961 ctccattggc ggcattgtgc atacgcactc gcgccacgcc accatctggg cgcaggcggg
    22021 tcagtcgatt ccagcaaccg gcaccaccca cgccgactat ttctacggca ccattccctg
    22081 cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt
    22141 catcgtagaa acctttgaaa aacagggtat cgatgcagcg caaatgcccg gcgttctggt
    22201 ccattcccac ggcccgtttg catggggcaa aaatgccgaa gatgcggtgc ataacgccat
    22261 cgtgctggaa gaggtcgctt atatggggat attctgccgt cagttagcgc cgcagttacc
    22321 ggatatgcag caaacgctgc tggataaaca ctatctgcgt aagcatggcg cgaaggcata
    22381 ttacgggcag taatgactgt ataaaaccac agccaatcaa acgaaaccag gctatactca
    22441 agcctggttt tttgatggat tttcagcgtg gcgcaggcag gttttatctt aacccgacac
    22501 tggcgggaca ccccgcaagg gacagaagtc tccttctggc tggcgacgga caacgggccg
    22561 ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta ttcacgccga tcaggttccc
    22621 cgcgctcagc atattttgca gggtgaacaa ggctttcgtc tgacaccgct ggcgttaaag
    22681 gattttcacc gccagccggt gtatggcctt tactgtcgcg cccatcgcca attgatgaat
    22741 tacgaaaagc gtctgcgtga aggtggcgtt accgtctacg aggccgatgt gcgcccgcca
    22801 gaacgctatc tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac
    22861 aatggcacta tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag
    22921 tgggtttcta tagatattga aaccacccgc cacggtgagc tgtactgcat cggcctggaa
    22981 ggctgcgggc agcgcatcgt ttatatgctg gggccggaga atggcgacgc ctcctcgctt
    23041 gatttcgaac tggaatacgt cgccagccgc ccgctgctgc tggaaaaact caacgcctgg
    23101 tttgcaaacc acgatcccga tgtgatcatc ggctggaacg tggtgcagtt cgatctgcga
    23161 atgctgcaaa aacatgccga gcgttaccgt cttccgctgc gtcttgggcg cgataatagc
    23221 gagctggagt ggcgcgagca cggctttaaa aacggcgtct tttttgccca ggctaaaggt
    23281 cggctaatta tcgacggtat cgaggcgctg aaatccgcgt tctggaattt ctcttcattc
    23341 tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa aatctatcga taacccgtgg
    23401 gatcgaatgg acgaaattga ccgccgtttc gccgaagata aacctgcgct ggcaacttat
    23461 aacctgaaag attgcgagct ggtgacgcag atcttccaca aaactgaaat catgccattt
    23521 ttactcgaac gagcaacggt gaacggcctg ccggtggatc ggcacggcgg ttcggtggca
    23581 gcgtttggtc atctctattt tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc
    23641 ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga tggattcacg gccagggctt
    23701 tatgattcag tgctggtgct ggactataaa agcctgtacc cgtcgatcat ccgcaccttt
    23761 ctgattgatc ccgtcgggct ggttgaaggc atggcgcaac ctgatccaga gcacagtacc
    23821 gaaggttttc tcgatgcctg gttctcacgg gagaaacatt gcctgccgga gattgtgact
    23881 aacatctggc acgggcgcga taaagccaaa cgtcagggta ataagccgct ttcgcaggcg
    23941 ctgaagatca ttatgaatgc cttttatggc gtgctcggca ccaccgcctg ccgcttcttc
    24001 gatccgcgcc tggcatcgtc gatcaccatg cgtggtcatc agatcatgcg gcagaccaaa
    24061 gcattgattg aagcgcaggg ctacgacatt atctacggag ataccgactc gacttttgtc
    24121 tggctaaaag gtgcacattc ggaagaagag gcggcgaaaa tcggtcgtgc actggtgcag
    24181 cacgttaacg cctggtgggc ggaaacgctg caaaaacaac ggctgaccag cgcattagaa
    24241 ctggagtatg aaactcattt ctgccgtttt cttatgccaa ccattcgcgg agctgatacc
    24301 ggcagtaaaa agcgctacgc cgggctgatt caggagggcg acaagcagcg gatggtgttt
    24361 aaagggctgg aaaccgtgcg caccgactgg acgccgctgg cccagcagtt tcagcaggag
    24421 ctgtacctgc gtatcttccg caacgagcca tatcaggagt acgtccgcca aaccatcgac
    24481 aaactgatgg cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg
    24541 ctgagcgaat atcagcgtaa tgttccgcct catgtacgcg ccgctcgcct tgccgatgaa
    24601 gaaaatcaaa agcgcggtcg ccccttgcaa tatcagaatc gcggcaccat taagtacgta
    24661 tggaccacca acggcccgga gccgctggac taccaacgtt caccactgga ttacgaacac
    24721 tatctgaccc gccagctaca acccgtggcg gagggaatac tcccttttat tgaggataat
    24781 tttgctacac ttatgaccgg gcaacttggg ctattttgag caaaaaaaag agttcgccag
    24841 ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc
    24901 ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat
    24961 taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata
    25021 cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac
    25081 ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg
    25141 tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag
    25201 aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt
    25261 ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg
    25321 accgtctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc
    25381 gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca
    25441 gcctgatccc gcatcagctc aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg
    25501 tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc
    25561 agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc
    25621 atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg
    25681 agcgttatgc cgaagctcag cacgatgctt acaacccgtt tgacaccgaa cagctggtga
    25741 tttgctcgct ggattttgcc cgtcgtagca aacagcgctt ggaacatctc tgtgaagccg
    25801 aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa
    25861 gccgtgaata tcaggccatt gaacaactgg cagagcacgt gccgggcgtt ctgctgctga
    25921 ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc
    25981 cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg
    26041 cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc
    26101 tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca
    26161 gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca
    26221 ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc
    26281 tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca
    26341 ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta
    26401 tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt
    26461 ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag
    26521 ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg
    26581 tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag
    26641 aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaactttc
    26701 agttcgccag tcacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc
    26761 gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc
    26821 tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg
    26881 agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact
    26941 atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaagc tgccgcgagc
    27001 aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact
    27061 ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata
    27121 ccaacctgat cgccttcgcc atgaacctgt tcgatattat cggtatcaat caggacgatc
    27181 gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg
    27241 gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag
    27301 atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt
    27361 ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta
    27421 cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca
    27481 accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg
    27541 cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg
    27601 gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag
    27661 cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa
    27721 aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg
    27781 acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg
    27841 caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa
    27901 aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga
    27961 tgaccatatt atggtggtca acaagccgag cggtttgttg tcagtgccgg gtcgtctgga
    28021 agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt
    28081 gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga
    28141 gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt
    28201 ctgggggcat ccatcccccg cagaaggtct ggtggatctg ccgctgattt gcgactggcc
    28261 aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga
    28321 agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttaccgggcg
    28381 ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt
    28441 ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt
    28501 gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta
    28561 atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg
    28621 tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta
    28681 tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg
    28741 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    28801 ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg tcggcttcac
    28861 gcccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc
    28921 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    28981 aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat acagcaccgc
    29041 ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa
    29101 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    29161 cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc
    29221 gccatgaagg ttcattcggt ccatcaactg gctggcgata tgaatatcag cctccgtgac
    29281 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    29341 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    29401 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    29461 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    29521 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg gtcaattgtg
    29581 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    29641 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    29701 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    29761 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    29821 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    29881 gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg
    29941 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    30001 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgtaccgttg
    30061 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    30121 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    30181 gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc tataccattc
    30241 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    30301 gcgaaattat tcatgaccgc gaagaacaag ttgctgagat ctggaacgcc cgctttaagg
    30361 tgggtccggt accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    30421 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    30481 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    30541 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    30601 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    30661 atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg gatcaggtgt
    30721 ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat gatttcgata
    30781 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    30841 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc gaacagaaca
    30901 ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat gatgttggtc
    30961 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    31021 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    31081 gcagcatcaa taccgaagcg aagtttctgg caacccatta tcagcaaacc aatcttgact
    31141 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta atgccgcaat
    31201 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    31261 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    31321 tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc cgggaccgga
    31381 cttacggcgg tcttgaccgt attgcctccg ctaaccaagt gacgaccggt gtcacatctc
    31441 gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa atctactatt
    31501 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct
    31561 cactggtgtg ggcaggcgac acctactggc gtatctccga gcgttgggga ttgcgtggcg
    31621 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    31681 gtcgggatga agaccgtctg gtacagctga attaccatta cgccagcccg gaatatattc
    31741 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    31801 taggtgctgt cgccagcagg ccaattgccg atcgttggtc cattgttggg gcctactact
    31861 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    31921 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    31981 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    32041 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    32101 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    32161 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    32221 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    32281 gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat
    32341 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    32401 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    32461 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    32521 ctgaactaca acacttatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    32581 aacaacgagg tgcgtcgtcg catcaccatc ctgccacagg aagtcgaatc cctggcgcag
    32641 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    32701 ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    32761 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    32821 cagcaggcgc tgaacggcgg ccagatgggg tggggccgta ttcaggagtt gccggggatc
    32881 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    32941 gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    33001 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    33061 gcccgcgtga aactggagca aattgctgct gatatcaaga gtggtaaaac gacttttgct
    33121 gccgcagcga aagagttctc gcaggatcca ggttctgcta accagggcgg tgatctcggc
    33181 tgggctacac cagatatttt cgatccagcc ttccgtgacg ccctgacccg cctgaacaaa
    33241 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    33301 acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg catgctgatg
    33361 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc cagcgcctac
    33421 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    33481 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    33541 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    33601 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg ggcacattaa
    33661 cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg attgaaaatg
    33721 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    33781 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaagc attccgttta
    33841 ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg
    33901 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    33961 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    34021 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    34081 aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc
    34141 gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    34201 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    34261 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    34321 cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    34381 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    34441 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    34501 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    34561 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    34621 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    34681 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    34741 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    34801 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    34861 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    34921 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    34981 caaagtggat tccgccgtcg tgcgcctggt ccctcatgca acgatgcctc acccggttaa
    35041 agatattcgc gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    35101 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta accggaatgg ggatcgaccc
    35161 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    35221 ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    35281 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    35341 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    35401 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    35461 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    35521 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    35581 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    35641 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    35701 gtagaattta cccccgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    35761 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    35821 ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg
    35881 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    35941 cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc ccacgcaggg
    36001 atcacgccgc agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    36061 ctgtcgagtg actcctatcc cttctttctt gacgctatgt acggcgatat gccaaataac
    36121 tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt
    36181 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagaa
    36241 gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc
    36301 atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg tatttacgcg
    36361 ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga agataaacag
    36421 tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca
    36481 cagcctgata caggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta
    36541 aattctatac aaacttaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    36601 gcatcggcgt cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    36661 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ttgcgctttt
    36721 ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca
    36781 cacgccgcaa tggcttcatc caccgatttc acccacgtta cgcgatcgtc cgtacccggt
    36841 tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaagta
    36901 tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    36961 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    37021 atcagactga tcattgagat ttcccgataa aaaaattgtc gccactatac gtaaagcgta
    37081 aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt
    37141 ccacgctctc cccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta
    37201 aagtgtgatt tacgtcactc tttattaaga tgagggtttc gtttccggtt catccgccat
    37261 gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag
    37321 atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt tatagaccgc
    37381 cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct gaatattaaa
    37441 gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag
    37501 cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctccggct tttcaacgcc
    37561 tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg
    37621 cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc
    37681 gttaatcagc acttccgctt tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc
    37741 atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt ccggatcgtg
    37801 atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa
    37861 acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat cggcttcacg
    37921 cgcctcctcc gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc
    37981 tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt
    38041 cgccatctgc gccgcaccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac
    38101 cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca gccacagcat
    38161 ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaat
    38221 cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc
    38281 tttaaacggt tcgatatcgc tttccaacgc atgacggtat tccgagcttg ccagcagtac
    38341 gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa
    38401 gccaaacaca aggaataacg ccacggcact aaacgcttcc cgcaagccag agcgggcaac
    38461 aaaacgcagc gccggacgcg taacatagcg ccccagcaat accaccagta ccagcgcacc
    38521 cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc
    38581 cagtagcggg atcatcgcca ccagcgggat tgccgcgata tcctggaaca gcagcactgc
    38641 aaaggcactg cggcccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc
    38701 aatcgccgtt gatgagagcg ccagcgtcat accgatcaat tccgcgacct gccagcgcag
    38761 cccaagtaac atacagaaca gccccagcag gccaccgcaa atcaccatct gcaatgcgcc
    38821 accgccgaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat
    38881 aataaacagc atcagcacca ccccaatctc ggcaaagtgc agaatagatt cggcatcggt
    38941 caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc caagtaccga
    39001 tcccagacca agacgtaccg caatgggtac aatcagcgcc gccgatccga ggtagatcag
    39061 cgcctgaacc agcgtatgac tatccatgat gggcctcctg ccattccagc agacgttgct
    39121 tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg
    39181 caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca
    39241 gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca
    39301 ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt
    39361 gcgacaaaac tttatcgatc caaagtttaa gaagcggagg aatgctgtac cactgcatcg
    39421 ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc ctgctcggcg gcaatatcga
    39481 tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtccttg
    39541 cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatga
    39601 tgaggatcat agttattagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca
    39661 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaaa
    39721 taatcccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acattcctcg
    39781 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    39841 tagcatcgtg ttactgatac cgtaattatt gataaatatc ggcagtgccc aggtcgaaac
    39901 aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt
    39961 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    40021 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatta gccccaaaac
    40081 cgccaacgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    40141 gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacatt
    40201 ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa ccaacgattt gcgggccaaa
    40261 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    40321 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    40381 atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
    40441 ccctttgcgt aataaccagc ggggagattc tggcagttcg aatcgaccaa tcaaaatcaa
    40501 caaacaaggg atcgccgcgc tacccagcat ccagcgccaa ccaccttcca catcataaag
    40561 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatcgcgg caataaagct
    40621 gatggaaaac gcccgctgac gggtactgga gaactcggtg atcatcgaag tggcgatggg
    40681 ataatctgca ccgatgacaa tgccgatgag tacccgcatc accaacagtt cgacggggga
    40741 tgaaacacac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    40801 tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa cgaacagccc
    40861 ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggagt
    40921 cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc
    40981 gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct
    41041 ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacgg cgttacgtat
    41101 caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact
    41161 gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt
    41221 aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca
    41281 ccagcagctc cagcacctgt ttatcagcat cggccttcac aacaatgtgc ggatgctctt
    41341 catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa
    41401 aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg
    41461 gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg
    41521 cgccacgccc accgccagtt ccgggttgcc gctaaacatg cgtgggttat caaggaacgc
    41581 cggtagtttc tggtacatgc gcatatcgcg cagcgggcca ctctccagat gctgacgata
    41641 ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc
    41701 tgcagcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat
    41761 acacattccg gcggcatcac cggcaatcaa tacgccgtca ccgaccagct ccggcagcat
    41821 gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc cgcccgcgat
    41881 cagcggtgca acggccggat gctgtttgaa atcttccagc atttgcggca ccgatttttt
    41941 cgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtat tttcattggt
    42001 ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc
    42061 gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta actcgatcag
    42121 ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc ccaacttttc
    42181 ggcaaggatg gagttcaccc catcagcaag gagcaccgtt ttcgcttcaa tcacatcacc
    42241 atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc
    42301 ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc aaccaggcat caaatttact
    42361 gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc agtagtccat
    42421 agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac
    42481 gggggcggaa tcggcgaaac ctggaataat gtgttccaga ctgtgggcat agagacgccc
    42541 gccagtaacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc
    42601 ttcgcgggcg agcaccagtg cggcaaccga accggcaagc cctgcaccga cgatgatggc
    42661 gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagagccgta atgcagccct
    42721 gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg gaaggatctt cacggcatcg
    42781 ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg
    42841 aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga tctacccgga gatccccacc
    42901 gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc
    42961 atccattttt cgttttctgc caccggacga gaacaggcca actccgcacc tatcgccttg
    43021 caaagctgtt ctgccagcgc aatgttctct ttgctgccaa tgccgcgacc gacgctgacc
    43081 accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg
    43141 gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg
    43201 tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca
    43261 atgcgttctt cgccaatcgc cagaccgccg taaaccatgt gtttcactgt tgctttaccg
    43321 tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt
    43381 tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca gcaccagacc gtcagcgccg
    43441 tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt cgatcatccg atcgtccggt
    43501 ttgccgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca
    43561 tcattgagga caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc
    43621 ggcagacgag agggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc
    43681 cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt
    43741 cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc tgttgttcag
    43801 accaggcgtc cacggcgtta aaaccaatat ccgccgccga ccatacctgg actggctttt
    43861 tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa
    43921 cagcaacaac cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc agttcgcgct
    43981 caacggtgag ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa
    44041 tattgaggat ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg gaagaaccat
    44101 cgccacagag gatcagatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg
    44161 tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccgggc
    44221 cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta
    44281 aggctgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt
    44341 tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa ccatcagcat
    44401 tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg
    44461 catctccaga aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt acagatatca
    44521 cgccctttaa tttggaaata aaactttaat caccaatatt gaaagtcaga tgctattttt
    44581 ttacccacaa aagaatataa caaactgatt aataaccaaa attaacgaaa aacgccctct
    44641 gttcactcga aacaaaaatg tgataccaat cacagaatac agcttattga atacccatta
    44701 tgagttagcc attaacgcgt ccacgaggtt aataataatt atattaaatg ttaacaaaaa
    44761 taaaacaaac gggaacgcaa aataaatatt cgttttcaca gtggaattaa ctcatgaaga
    44821 atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccatta ataatcgtcg
    44881 gcatactttg ttggcttaca gtcagagatc tggatgcggc gaatgtcgtt attaatgctg
    44941 tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg gtggtgatgc
    45001 ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac
    45061 cgccagaatt tagcaccgcc agttggatct ttatgatgtt cgcctcctgt acgtctgctg
    45121 ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg ccgtttggct
    45181 tagaaccgaa ctcgacagga gcgaaagagt tggggctggc ttacagcttg ttccactggg
    45241 gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac ttcttctttg
    45301 tccgcaaaat ggaagtgatt cgccccagct cgacactggt gccgctggca ggtgaaaaac
    45361 acgccaaagg gttgttcggt accatcgtcg acaacttcta tctcgtcgcc ttgatcttcg
    45421 cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg caatggttgt
    45481 ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg attatcctca
    45541 acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta
    45601 gttacctgag cttcctgatg ctgggttggg tgttcatcgt cagcggtgcc agcttcatca
    45661 tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcgc atgttgttct
    45721 ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc tactgggcat
    45781 ggtgggtgat ttacgccatc cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta
    45841 ctgtgcgtga actgtgcttc ggcatggtga tggggctgac agcatccacc tggatcctgt
    45901 ggaccgtgct cggtagtaac actctgctgt tgatggataa aaacatcatc aacattccaa
    45961 atctgatcga acagtacggt gtggcgcgcg ccatcatcga aacctgggcc gctctgccac
    46021 tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc gttacgctgg
    46081 ttaacgcctg ctcttatacc ctggcgatgt ctacttgccg cgaagtacgc gatggtgaag
    46141 aaccaccgct gctggtgcgt atcggttggt caattctggt tggcattatc ggtattgttc
    46201 tgctggcgct cggcggcctg aaaccgattc aaaccgccat tatcgccgga ggatgcccgc
    46261 tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa cagaactgga
    46321 aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat ttaaatgatg
    46381 agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac tgggaggcct
    46441 attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa gcactggcgg
    46501 atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac gcggggtttg
    46561 ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc tatgtactgt
    46621 accagctgcc gggcgggttc aataccttcc tgcgcgaagg cacacaagag cagatcgaca
    46681 agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt accgaaccgg
    46741 gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat ggtaagattt
    46801 atcttaatgg tagtaagtgt tttattacca gtagcgccta caccccgtac atcgtggtga
    46861 tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt gttgatatga
    46921 gcaaaccggg catcaaagtg accaaacttg agaagctcgg tctgcgtatg gatagctgct
    46981 gtgaaatcac ctttgacgat gtggaactgg acgagaaaga catgttcggt cgggaaggta
    47041 acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta gccctgacca
    47101 actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac cagcgcgtgc
    47161 agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc cacatggcga
    47221 tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca gacaacggca
    47281 ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg gcatttgaag
    47341 tggtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca
    47401 gccgcttctg gcgtgacctg cgtgtagacc gcgtttccgg aggatctgac gaaatgcaga
    47461 tcctgacgct gggtcgtgcg gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg
    47521 cgacgctgac gcgtcttatc aggcctacgg gagcacgaat gtaagccgga taaggcgttt
    47581 acgccgcatc cggcagtcat gcgctacata aatttttcag gagctaataa tggatcatct
    47641 acccatgccg aaattcggac cgctggccgg attgcgcgtt gtcttctccg gtatcgaaat
    47701 cgccggaccg tttgccggac aaatgttcgc agaatggggc gcggaagtta tttggatcga
    47761 gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg
    47821 caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag cgtttctgaa
    47881 attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg
    47941 tggcattacc gatgaagtac tgtggcagca caacccgaaa ctggttatcg ctcacctgtc
    48001 cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata acaccatcgc
    48061 ccaggccttc agtggttacc tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt
    48121 cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc tggcagcact
    48181 gcataaagtg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt atgaagtgat
    48241 gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg
    48301 catgagcaaa ggtaaagatc cctactacgc cggttgcggt ctgtataaat gtgccgacgg
    48361 ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta aagatattgg
    48421 cctcgcacat ctactcggta cgccggaaat cccggaaggc actcagctta tccaccgtat
    48481 cgaatgccct tacggcccac tggtagaaga aaaactcgat gcctggctgg cggcacatac
    48541 catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca aagtactgac
    48601 cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca ctcagtggca
    48661 aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca aaaataaccc
    48721 cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca ttttgaaaaa
    48781 tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg ccaaagttga
    48841 ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa
    48901 caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt caaatgtggg
    48961 acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag
    49021 tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg gcaaacctgt
    49081 tttatacgct ggggattcgc aaaggcaaca aggttgcact acatctcgac aactgcccgg
    49141 aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg ccgattaacg
    49201 cccgcctgtt acgcgaggaa agcgagtgga tcttgcaaaa tagccaggcg tgcctgctgg
    49261 tgaccagtgc gcaattttat cctatgtatc aacagattca gcaggaagat gccactcaat
    49321 tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggagtg agttcattta
    49381 ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta tcgactgacg
    49441 atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt gtggtgatta
    49501 cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca ctgcgtgacg
    49561 atgacgtcta catgacggta atgcctgcgt ttcatatcga ttgccagtgt actgcggcga
    49621 tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc gcccgcgcct
    49681 tctggggaca ggcgcagaag taccgcgcca ccattaccga atgtattccg atgatgatcc
    49741 gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg cgggaagtga
    49801 tgttttatct caacttgtcg gagcaggaaa aagacgcatt ttgtgaacgc ttcggcgttc
    49861 gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc gatcgcccta
    49921 gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa gcggagatcc
    49981 gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg
    50041 taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc
    50101 tggaagccga cggctggcta cataccggcg ataccggata ccgcgacgaa gaaggctttt
    50161 tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg
    50221 tggagctgga aaatatcatc gccacccatc cgaaaattca ggacatcgtg gttgtgggta
    50281 ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa
    50341 cattgagcga agaggaattt tttcgcttct gcgaacaaaa tatggcgaaa tttaaagtgc
    50401 cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa ataattagaa
    50461 agaatctgaa ataaatgaaa cgtcagggaa ctaccctgcc agctaataat cacgccctaa
    50521 aacaatatgc attttttgca gggatgttgt cgtccctaaa aaagcaaaaa tggagaaaag
    50581 gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt acccttgatc
    50641 gcccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa
    50701 atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga gagaaattct
    50761 tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat gctgactttg
    50821 gcccgggtgg ttttgcggga ttaaccgaaa ttttcaatct cgacaaacca gttatcgcag
    50881 ctgtgaacgg ctacgccttt ggcggcggtt tcgaactggc gctggcggca gattttattg
    50941 tttgtgccga taacgccagc ttcgccctgc cggaagccaa actgggcatc gttcctgaca
    51001 gcggcggtgt gctgcgtctg ccgaagatcc tgccgcctac catcgtcaat gaaatggtga
    51061 tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg
    51121 ttagccaggc ggaactgatg gataacgccc gcgaactggc tcagcagctg gttaacagcg
    51181 ccccgctggc gattgcggcg ctgaaagaga tctaccgcac caccagcgaa atgccggtag
    51241 aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt
    51301 cggaagatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag
    51361 gacgttaaca accatgagct attacgcctt tgaggggtta attccggtgg ttcacccgac
    51421 ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta
    51481 catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc
    51541 caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca tcgttgggga
    51601 aaacggccat atcgggcatg gagcgatcct gcatggttgt gtgattggtc gcgatgcatt
    51661 ggtcgggatg aacagcgtaa ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc
    51721 cgcaatgagc tttgtcaaag caggctttag tggcgagaaa cgccagttgt tgatgggtac
    51781 gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac ggttgaatac
    51841 caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact
    51901 gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga cgcctaaacg
    51961 gtgatcttgc cggatgcgac ctggttgctg gataagatgc tacaacatcg tatctggcaa
    52021 gcaggacgcc cggtatcaac gagataaaat taacgacaca tactctttga caacatcatc
    52081 tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggtgtcttt
    52141 cttacgccga ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg
    52201 atatcgacca ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg
    52261 atcatcttaa cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta
    52321 accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga
    52381 ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca
    52441 cacatacgaa ctccagtttc acaaattaaa agtctagcta tgactcgttt tgaagcaatt
    52501 aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag
    52561 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
    52621 gggcaaaaaa aaacggtcct catcagagaa ccgtattagt tattcgatca aattagcgga
    52681 tgaaaaatat ccgccacgac acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa
    52741 attacttttt cagtcgcatc ggcattcagc gccatcgcgg tagcgaaacc accgttcagg
    52801 gtggtgtcat aatgcacttt atattgcagc gcactgcgac ggatcacgcg ggagtcttca
    52861 atcgcacgac ggcctgaggt ggtgttgatg atgtaggtat attcgccatt cttgatacgg
    52921 tcctgaatgt gcggacggcc ttcatgcacc ttgtttacca gacgcggatt gatacccgct
    52981 tcgcccagca caatcgccgt gccgtgggtc gcatccagct cgaagccctg tttcagcagt
    53041 tttgccgcca ggtccaccac gcgttcttta tcgccttcgc gcacggaaag cagcgcacga
    53101 ccgtgtttct tcatggtgga gttgctgccc agctgcgctt tggcgaacgc ttcagcgaag
    53161 gtgcggccca cgcccatgac ttccccggta gagcgcattt ctggccctaa cagcgggtca
    53221 acgcctggga atttattgaa cggcagcacc acttctttca ccgagtagta cggcggaata
    53281 acttctttgg ttacgccctg ctcagccagc gatttgccag ccatcacacg cgccgccact
    53341 tttgccagcg gtacgccggt ggctttggag acgaacggaa cagtacgcgc cgcacgcggg
    53401 tttacttcaa tcaggtaaac ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg
    53461 cgcacctgca attcgaaggc cagtttctgc acctgctggc gcatcacatc ctgaatttcc
    53521 tgacttaagg tgtacgctgg cagcgaacat gcggagtcac cggagtgcac acccgcctgc
    53581 tcgatgtgct ctatgatgcc gccaatcagc accatttcgc cgtcgcagat ggcatccacg
    53641 tcaacttcta ctgcgtcatc aaggaaatga tccagcaaca ctggcgcatc gttagacacg
    53701 ctaaccgccg tctggaagta gcgacgcagg tcagcttcgt catagacgat ttccatcgcc
    53761 cgaccgccga gaacgtaaga cggacgcacc accagcgggt agccaatctc ttttgccttc
    53821 tcaactgcca tttcaatagc agtaacggtg gcgttcgccg gttgtttcag tttcagacgg
    53881 tcaaccgcat gctggaagcg ttcacggtct tccgcacggt cgatagcatc cgggctggtg
    53941 ccgataaccg gtacgccagc ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg
    54001 ccgtactgaa cgataacgcc tttcggcttc tcaatacgca cgatttccag cacatcttcc
    54061 agagttaccg gctcgaagta gaggcgatcg gaagtgtcgt agtcggtaga gacggtttcc
    54121 gggttacagt taaccataat ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt
    54181 acgcagcagt agtcgaattc gataccctga ccgatacggt ttggaccgcc gccaagcacc
    54241 atgatttttt cacggtcggt agacggattc gcttcgcact cttcttcata agtggagtac
    54301 atgtaagcgg tgtcggtggc aaactctgcc gcacaggtat ccacgcgctt gtagaccggg
    54361 tgcaggtcat attggtcacg cagcttacgg atttccgctt cgcgtacgcc cgccagtttt
    54421 gccaagcgcg catcggcaaa gcctttgcgt ttcagctggc gcaggaattc agcgttcagg
    54481 ccagtgatgc ccacttccgc cactttctct tccagacgca ccagttcttc aatctgtacc
    54541 aggaaccagc ggtcaatgtt agtcaggttg aagacgccgt ccacagacag gcccgcgcgg
    54601 aaagcatcgg cgatgtacca gatacgctct gcgcctgcgt ctttcagttc gcgacggatt
    54661 ttggttaacg cttccgggtc atccaggctc actttcgggt cgaagccagt tgcgcccact
    54721 tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg tgcgaccaat cgccatcact
    54781 tcgccaaccg atttcatctg agtggtcaga cggtcgttag caccggcgaa tttttcgaag
    54841 ttgaagcgag gaattttagt aaccacatag tcgatggacg gctcgaagga ggccggagta
    54901 cgtccgccag tgatgtcgtt catcagttcg tctagggtgt aacccaccgc cagtttcgcc
    54961 gccactttag caatcgggaa accggtcgct ttcgacgcca gcgccgaaga acgggacacg
    55021 cgtgggttca tttcgataac aatcaggcga ccgtttttcg ggttcaccgc aaactggaca
    55081 ttggaaccac cggtttcaac gccgatttca cgcagcaccg ccatcgaggc gttacgcatg
    55141 atttgatatt ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga gtcaccggtg
    55201 tggatgccca tcgcatcaaa gttttcgata gagcagacga tgatgcagtt gtcgttttta
    55261 tcacgcacca cttccatctc gtactctttc cagccgatca gcgactcatc aatcagcaac
    55321 tctttggtcg gagagagatc cagaccgcgg gcgcaaattt cttcaaactc ttcacggtta
    55381 taagcgatac cgccgccgct accgcccatg gtaaaggatg ggcgaataat gcacgggaag
    55441 cccacgtcag cggcaacagc cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg
    55501 gtttccagac caattttctt catcgctacg tcgaaacgac ggcggtcttc tgctttatca
    55561 atcgcatcgg cagtggcacc aatcatggtg acaccgaact cttccaacac gccctgacgt
    55621 tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca tggttggcag caccgcgtcc
    55681 gggcgctctt tttcaataat cttgcgtacc acttcccagt gaatcggctc gatgtaggtc
    55741 gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt tggagttcac cagaatgacg
    55801 cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc cagagtagtc aaactcacac
    55861 gcctgaccga taacaatcgg gcccgcaccc agaatcagga tactttttat atctgtacgt
    55921 tttggcatgg ctcttttact cctgattact tagcggtttt acggtactgc tcaattaact
    55981 tgataaagtg gtcgaacaac ggcgcggcgt cgtgtggacc agggctggct tcagggtgac
    56041 cctggaagct gaacgccggc ttatcgatgc gatgaatgcc ctgtaacgta ccgtcgaaca
    56101 gggatttatg cgtgacacgc aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt
    56161 ggttctgggc ggtgatcatc accacgtttt tctccacatc tttaaccgga tggttgccgc
    56221 cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc agcagctgat
    56281 gaccgagaca gatgccgaat accggaatat cggtttcgag gaacttctgg atggcggtaa
    56341 tggcgtaatc gcacggggcc gggtcgccag gaccgttgga gaggaagatg ccgtctggat
    56401 tcattttcag cacatcttcc gcagaagttt gcgccggaac gatggtcagg cgacagcctc
    56461 tatccaccag catccgcagg atgttgcgct tggcaccaaa atcataagcc acgacgtgga
    56521 acggcagctc gtcttctttt ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt
    56581 gtgtccagct ataggcttct gcggtggtca cttcttttgc cagatccatg ccgttcagac
    56641 ctgggaacgc gcgggctttt tctaacgcca gcgccgcatc cgggttatcg cctgcgataa
    56701 tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat
    56761 cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga gaggtcttcg gtattacgga
    56821 agttgctggc aatcagcggc aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag
    56881 attcttcatc ggcgtcatta gtgccgacat tgccaatatg gggataagta agagtaacga
    56941 tttgacgaga ataggaagga tcagtgagga tttcttgata accggtcatt gaagtattga
    57001 aaacgacttc cccaaccgcc gaacctgttg cccctatggc ccgaccgtga aactgggttc
    57061 cgtcttccag aaccaatagc gctgacttaa tcaaaacacc ctccagagaa tactcactca
    57121 ctttatttgc atattaattc acagtgatga cgtaaatcaa tgcaaatctg cttaccgacg
    57181 aatttctggc aaacggcggc attctggaga tatgggcgct aaaagtcaac ttaatggcag
    57241 gattttttat attattttgt ggctcatgca tgttttacag gcattaagcg acaaaaacat
    57301 catctaacct gttctggaaa acgcttgcgc agcaagaaga ttgcatgaaa taaaaacgag
    57361 caggaaaaag tggaccaaat ggtcaaaatt tacattaaaa caacaaaaat agaagacgaa
    57421 acatgtaatt tttggcagtt atagaaaatt taaaagcata acaccaaaaa taaaaggact
    57481 aaaaattaaa gccctttata attaataaat taaatgtgtt acttttgcat cataataaac
    57541 attttgtggt tacaaattat tgagatcaag cacatctcgc atatcaaaaa gaccactttc
    57601 cttaccactc aaccacaaag ccgatcttac cgcaccgtta gcaaatgtca tgcggctgga
    57661 cgccttatgg gtgatctcca gacgctcgcc aatatcggca aacatcgcgg tatgttcgcc
    57721 aacgatgtca cctgcacgca cggtagcaaa accaatggta cctggcacac gttcaccggt
    57781 atggccttca cgactgtaga ccgcgcaatc tttcagatct ttatcaaggg cgtgggcgat
    57841 cgcctctccc attgccagtg cggtgcctga cggcgcatca actttatgtc tatgatgtgc
    57901 ttcaataatt tcgatatcgg tgtagtcacc catcactttg gctgctttct ccagcagctt
    57961 aagcatgacg ttaacgccaa cgctaaaatt ggcggcaaag acaatcgcaa tatcggcggc
    58021 ggcgtcacga attgcctgtt taccggcttc gtcaaacccc gtagtgccga tcaccatccc
    58081 tttgccatgc tgccgacaaa aagcgagatg gttcagcgta ccttccggac gggtaaaatc
    58141 gataaacaca tcaaaatcat cttttatcgc atcaagactg ctttgcacgg taacgcctgt
    58201 tttccctgca ccagccagct caccggcgtc gctgcccagt aaagaagatc cttcgcgctc
    58261 cagcgccgcg cccagctgca cgccttctaa tgccagtgcc gcctgaatca actggcggcc
    58321 catacgcccc ccggctcccg cgatggcaac gcggatgttt gcatcatgca tagctattct
    58381 cttttgttaa tttgcataga ccgttttcag agtaaccagc gcatcgctac gccgccagcc
    58441 aaaaacgctg ataattagaa attaatgata aacagggagg gatatcagtg aaaggcatga
    58501 gtcgatgaat gactgcatgc cgataacatg tgacaggtta cggcaccaga gccagcacct
    58561 cagccaccca ctgttggaag ccttttacat tcagatccaa tgccacctgt acattggctg
    58621 gtttgcccag gcaaccgtcg atatcaacca ccgtcgtgcc tgaggtaaat tcgccttgag
    58681 tttctaccgc gacaaaacac ggcttgaggg tgaataagtc cgggcgcacc agccaggcga
    58741 tggcgcagag atcgtgcatt cgcagaccgc tttgcatact gccgctacgg tagtggctaa
    58801 acagggcgtg gagcattttt ccggtgcgat tcaactccgg taaagtagcg agatagtcag
    58861 gggttaatat tgcctgattg gtgacatcca gaccgcacat gacaatttcg atgccgctac
    58921 ggaagacaca ggcagccgct tctggatcgg cagcgatatt aaactcggca tttggtgtac
    58981 agttgccgcg accagcagaa ccgcccatga tcaccagacg acgaatatac ggtttacatt
    59041 ccgggcattg taaaagtaac agcgcaatat tggttaacgg gccgatggcc accagggtaa
    59101 cgggctctgg tgcacgcatc agggcatccc gaatcgccag aaacgccggt atcccgagtg
    59161 gctttcggtt gtgctcaaca aagtcgtagc cagccattcc cgattcgccg tgcacagatg
    59221 ctgcatcacg cggtgcgcgt accagcggca cagcggctcc ctgggcgagc ggaatctccg
    59281 cattccagaa atgcagcagt tgcagggcat tgcgggtagt tttctcaacc gagacattac
    59341 ccgcgacggt ggtcatcagt tgcaggtcga gttcgggggc aaaaatcgcg gcggcaatgg
    59401 cgacggcatc gtcaatgccg gggtcagtat cgaggaagat aggtaaacgc atgttttctt
    59461 cataaaaaat gccggtaaca agaccggcat tttcgcataa cttaggctgc taatgactta
    59521 atcgacttca cgaatatcga cacgcagctc tttcggcact tcgaaaacaa tattttcttc
    59581 acggccttcc agcggaatgg cttcaccacc acccaactgc tgcaaacgtg ccaccacatt
    59641 ctgcaccaga atatccggag ccgatgcgcc cgcagtcacg ccgacgcatt taacctcttt
    59701 cacccactct tcctggatat ctttcgcatc gtcaatcaaa aacgcgtgtt tgcccatacg
    59761 ctgggccagc tccgccagac ggttggagtt ggaggagttt ttcgaaccga ccaccaacac
    59821 aacttccgcc tgttctgcca gggcgcgtac cgcttcctga cggttagtcg tggcgtagca
    59881 gatgtcatct ttgcgcggac cgacaatttt cgggaagcgt ttacgcagcg cgtcgatcac
    59941 atcagacgtg tcatccaccg acagcgtggt ttgggtcata aaggagagct tctcttcgtt
    60001 tttgaccgtc agtttccaca catcgtctgg cgattcgact agatacattc ccccttcggg
    60061 gttgctgtac tgccccatcg tcccttccac ttccgggtgc ccggcgtggc cgatgagaat
    60121 agattcttcg ccacggcgac tggcgcgggc aacttccata tgcactttgg tcaccagcgg
    60181 acaggtggca tcaaacaccg tcaaatcgcg gcttttcgct tcgttacgaa ccgcctgaga
    60241 aacaccgtgt gcggagaaaa tcaggatcgc gccgtccggc acttcgctaa tctgctcaat
    60301 aaagatagcc ccacgctcgc gcaagctatc gaccacgtag cggttatgca ccacttcgtg
    60361 acggacatat atcggtgcgc cgtaaatggc cagcgcgttt tcaacaatgc tgatagcgcg
    60421 gtctaccccg gcacaaaaac cacgcgggtt ggccaacagg atctgcatgt tacgcctcca
    60481 gtgccggatc gatttccagc acttcaatat caaaatgaac ggtctgcccg gccagcggat
    60541 ggttgaaatc aacggtaatg gagtcgccgt taatttcgcg gatcacacca ggcatctcac
    60601 tgccatccat tgcggtaaaa agcatgattg cgccaatttc tggctcgcct gcatccataa
    60661 attcacggcg ggagaagtac tgaatcaggt ccggtgacgg cacgccaaac gccgcatctg
    60721 gctccagcga gaaggtggtt ttgtcgccca ctttcagccc cagcaggtgt tgctccagcc
    60781 cttcagaaag agaagcatcg cccaggcgga acagcgccgg tttaccgttg ttgcgagtag
    60841 actcggcggt ggtgccatcg tcgagtttta gcgtgaagtg caccaggacg gcgctattgc
    60901 tctgtacaga ttcagacatg caggtttgct cttatttttg aggatttttg ccggatgcgg
    60961 cctacgattt cgtagcaatc tgtaggccgc atccggcagg atttattatt gttttttcgc
    61021 tttggaaggt aaaaaccctt ccagcacgat cagtgccgca ccgacacaaa tggcagtatc
    61081 ggcaaggttg aaggtggcga agtgccagtc gccgacgtag aagtcgatca tatcgacaac
    61141 gaagccgtgc cacaggcggt cgaacaggtt gcccagcgcg ccgccaataa tcagcgcgta
    61201 agcgatattg tttagcttct gcgtggcctt cgagcgatac atcatcactg ccaggagcac
    61261 gctaatacca atcgcaatac cggcaaagaa ccaacgctgc cagccgccgc tatcggcaag
    61321 gaaactaaac gccgcgccat agttacgcgc ataatgcaga ttaagcgacg ggaacagcgg
    61381 gaccgtatcc cccagagcaa agttctggag gatcaggtat ttgctgccca gatcgataat
    61441 cagcacgact accaccagcc acagccagcg tagccctgtt gaacagatcg attgactcat
    61501 caggcaaact tacgtttttc accgtcaccg gcgacgttgc tgacacagcg gccgcagatt
    61561 tctgcgtgtt ccgccacctt gccgacatcc tgggtgtagt gccagcagcg tgggcacttc
    61621 tcaccttcgg ctttactcaa cgcgactttc agccctttga gtacttcgct ctgctgagca
    61681 tcagcaggtg cgtcgttata gtctgcaacg gtagcgccgg aggtcaacag gacaaatcgt
    61741 aattcatcgc ccagcgcggt cagtttcgcc gccagttccg gttctgcata caaggttact
    61801 gccgcttcca gcgagccacc cactttcttg tcggcacgcg cttgctcaat gactttgttc
    61861 acttcgccac gcactttcaa cagctcgtcc cagaacgcat cgttcattgc ttcactgtct
    61921 gccagaccaa acaggccttc gtaccactca ccggtgaaga cgtatttttc acgttcgccc
    61981 ggcagatagc cccacacttc atcagcggtg aaggagagga ttggtgccat ccagcgcacc
    62041 agcgcttcag cgatgtgata cagcgcagtc tggcagctac gacgcgccac gctatccgct
    62101 ttggcggtgt actgacggtc tttgatgatg tcgaggtaga aggaacccat ctcaacggag
    62161 cagaagcgca tcagacgctg taccacttcg tggaaatcgt atgcttcgta cgccttgagg
    62221 atgtcttcct gtgccgcttt cgcacaacct acggcccagc gatccagtac caccatctct
    62281 tccggtttca ccatatcttt tgctggatca aaaccattca ggtttgccag caggaagcgc
    62341 gcggtgttac ggatacgacg atagctatcg gcagcacgtt tcaggatctc gtcagaaacg
    62401 gccatttcac cggtgtagtc ggttgatgcc acccacagac gcagaatatc cgcgcccagt
    62461 ttgttcatca catcctgcgg cgaaacggtg ttgccgatgg atttagacat cttgcgaccc
    62521 tgaccatcca cggtaaagcc gtgggtcagt acctgacgat acggcgcttt gcctttcatc
    62581 gcggtggaga tcattaggga agacatgaac caaccacggt gctggtcaga accttccaga
    62641 tacatgtccg ctgcgtgacc ggcaaattcc ggacgcacgt caacaacaga agagtgggta
    62701 gatccggagt caaaccatac gtcgagagta tccggcactt tcacatactg gtcagcttcg
    62761 tcgccgagga tctctttcgc atcgagatcc caccacgcct ggatgccgtc aacttcaacg
    62821 cgtttagcca cttcttccat cagttcgagg gtacgcggat gcagctcttc cgtgtctttg
    62881 tgcacgaaca gtgacatcgg tacgccccag gtgcgctgac gggagataca ccagtcagga
    62941 cggttagcaa ccatcgactc gatacgcgcc tggccccagt ccgggagcca ctgcacgcct
    63001 ttgatctctt tcagtgactg cgcacgcaga cctttctgat ccatgctgac gaaccactgc
    63061 ggtgtcgcgc ggaagatgat cggcgttttg tgacgccagc agcacggata gctgtgctgc
    63121 attttctcaa cgtgcagcag cgcgcctttt tcctgcagca gcgcaacgac gatgtcgttc
    63181 gctttgaaga cgttcacgcc atccagcgtc ggataagtgc ccggcagata agtgccgtcc
    63241 gggccaaccg ggttagcggt ttccaggccg tatttctgac cgatcacata gtcgtccggg
    63301 ccgtggccag gcgcggtgtg aacggcaccg gtaccggcat ccagggtaac gtgatcgccg
    63361 aggattgccg gaacgtcgaa gcccataaac ggatgggtaa agcgcagcag ctcaagctct
    63421 gcacctttta ccgtgccgag aatggtgtaa tcggtcacgc cgatacgctg cattacgctt
    63481 tcaaccagat ctttcgccag aatcacggcc tgaccgtcga tctgcaccag cgcatagtcg
    63541 aaatcaggtg caatagagat tgcgcggttc gcaggcagag tccacggcgt agtggtccag
    63601 atcaccagcg agattgggcc gttaacgttg cttacgccaa atttcgcttt cagcgcatcc
    63661 tgatcgaccg cctggaaagc gacgtcaatg gacggagaag ttttgtcgta atactcaact
    63721 tccgcttctg ccagtgcaga acggcagtct acgcaccagt gcaccggctt cgcgcctttg
    63781 tgcaggtgac cattgccgat gattttgccc agcgcgcgga tgatgttggc ttcagttttg
    63841 aagtccatag tcagatacgg gtgcgaccag tcgcccagca cgcccagacg gataaagtct
    63901 ttgcgttgac cgtcaacctg ggtcgccgcg tattcgcggc acttggcgcg gaactcggcg
    63961 gcggtgaatt tctcacccgg cttaccgtat tcttgctcta ctttcagctc gatcggcaga
    64021 ccgtggcagt cccagccagg cacatacggc gagtcataac cggaaagccc tttggacttc
    64081 acgataatgt ctttcagaat cttgttaacc gagtgaccaa tatgaatgct gccattcgca
    64141 taaggagggc catcatgcag aatgaaggtt tttttgcctt ttttagccgc acggatgatg
    64201 ccgtacagat catcatcagt ccaacgcgcc agcattcccg gttcgcgctt ggcgagatcg
    64261 ccacgcatcg ggaaccctgt ttccggcaaa ttcagggttg atttatagtc actcatcaga
    64321 ttctcggttc cgtgtttcgg tttgattaca taacaggctt aagccggttt tgttagccca
    64381 aaaaattcgc gggcggttaa ttcatcacgc gcaatctgcg ctttcagttc gtccagcgac
    64441 gcaaatcgct gctcattgcg tattttttta cgcagcacta cttgtatatg gcgaccgtaa
    64501 aggtccattg caacatctaa caaatgcact tccagctgct ggcgaatacc ggcaaccgtt
    64561 gggcgtgttc cgatgtttgc cacgccgggt aacggctttt caccgaggcc cagcacttct
    64621 accgcataaa cccctttcac cggggaaacc tgacggcgta gcggtacatt cgccgtcggg
    64681 aaacctatag tgcgccctaa ttcatcaccg tggactacac gcccggagat ggcaaacggg
    64741 tgccccagta aactttctgc cagagccaga ttgtcatcag ccagcgcctg acgcacggcg
    64801 gtgctgctga tacgcacacc accttcgcaa aaagtttgcg tactggtgat atcgaagccg
    64861 tattccatgc cagctttctg taataacaag aaatcgcctt cacgaccagc gccaaagcgg
    64921 aaatcatcac ctacggcaag aaattttacg cgcaaatgct tcaccaaaag atcgctgatg
    64981 aaattttgcg cggttaacgc cgcaaaacgc ctgtcgaaac gcacgcacag cacgtaatca
    65041 acgccacact ctgcaaggta acgcagtttt tcccgcagcc gggtcagtct tgccggggct
    65101 ttatcggtag caaacagttc cagtggttga ggttcaaaaa gcatcaccat caccggtaag
    65161 ttgcgcttgc gcccttcttc ctgcaagccc tgtaacagcg cgcgatgacc gcgatgcacg
    65221 ccgtcgaaat taccaatagt cagcacacac ccttcttgcg gggcctggct gagattatgt
    65281 atgccgcgta tcagcttcat gtctggctca aaacagtgaa aatcgtccga gtataccttg
    65341 tacagcggtc aaggttaacc ggcgattgag taccgaaatc agaaagaagt ggcttttcat
    65401 tgccatggag caaatcacgg gaagaaactg accgcctgct gcaattttta tcgcggaaaa
    65461 gctgtattca caccccgcaa gctggtagaa tcctgcgcca tcactacgta acgagtgccg
    65521 gcacattaac ggcgcttatt tgcacaaatc cattgacaaa agaaggctaa aagggcatat
    65581 tcctcggcct ttgaattgtc catatagaac acatttggga gttggacctt ggct
//
LOCUS       DAJVPT010000009        60234 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVPT010000009 DAJVPT010000000
VERSION     DAJVPT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..147
                     /locus_tag="OQM47_000567"
     CDS             <1..147
                     /locus_tag="OQM47_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCS1478847.1"
                     /translation="DTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            260..1054
                     /gene="panB"
                     /locus_tag="OQM47_000568"
     CDS             260..1054
                     /gene="panB"
                     /locus_tag="OQM47_000568"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1478848.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1066..1917
                     /gene="panC"
                     /locus_tag="OQM47_000569"
     CDS             1066..1917
                     /gene="panC"
                     /locus_tag="OQM47_000569"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1478849.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(1991..2893)
                     /gene="rpnC"
                     /locus_tag="OQM47_000570"
     CDS             complement(1991..2893)
                     /gene="rpnC"
                     /locus_tag="OQM47_000570"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1478850.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3167..3547
                     /gene="panD"
                     /locus_tag="OQM47_000571"
     CDS             3167..3547
                     /gene="panD"
                     /locus_tag="OQM47_000571"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1478851.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3551..4780)
                     /locus_tag="OQM47_000572"
     CDS             complement(3551..4780)
                     /locus_tag="OQM47_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1478852.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4844..5284)
                     /locus_tag="OQM47_000573"
     CDS             complement(4844..5284)
                     /locus_tag="OQM47_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1478853.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5389..6159)
                     /locus_tag="OQM47_000574"
     CDS             complement(5389..6159)
                     /locus_tag="OQM47_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1478854.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6156..7082)
                     /locus_tag="OQM47_000575"
     CDS             complement(6156..7082)
                     /locus_tag="OQM47_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1478855.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7191..7853
                     /gene="can"
                     /locus_tag="OQM47_000576"
     CDS             7191..7853
                     /gene="can"
                     /locus_tag="OQM47_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1478856.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7894..8430)
                     /gene="hpt"
                     /locus_tag="OQM47_000577"
     CDS             complement(7894..8430)
                     /gene="hpt"
                     /locus_tag="OQM47_000577"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1478857.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8636..11026
                     /gene="gcd"
                     /locus_tag="OQM47_000578"
     CDS             8636..11026
                     /gene="gcd"
                     /locus_tag="OQM47_000578"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1478858.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPSNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11073..12623)
                     /gene="cueO"
                     /locus_tag="OQM47_000579"
     CDS             complement(11073..12623)
                     /gene="cueO"
                     /locus_tag="OQM47_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1478859.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12789..13136
                     /locus_tag="OQM47_000580"
     CDS             12789..13136
                     /locus_tag="OQM47_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1478860.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13242..14108
                     /gene="speE"
                     /locus_tag="OQM47_000581"
     CDS             13242..14108
                     /gene="speE"
                     /locus_tag="OQM47_000581"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1478861.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14124..14918
                     /gene="speD"
                     /locus_tag="OQM47_000582"
     CDS             14124..14918
                     /gene="speD"
                     /locus_tag="OQM47_000582"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1478862.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKARYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14956..15318)
                     /locus_tag="OQM47_000583"
     CDS             complement(14956..15318)
                     /locus_tag="OQM47_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1478863.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15493..18090)
                     /gene="acnB"
                     /locus_tag="OQM47_000584"
     CDS             complement(15493..18090)
                     /gene="acnB"
                     /locus_tag="OQM47_000584"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1478864.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18445..20298
                     /locus_tag="OQM47_000585"
                     /pseudo
     CDS             18445..20298
                     /locus_tag="OQM47_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20369..21793)
                     /gene="lpdA"
                     /locus_tag="OQM47_000586"
     CDS             complement(20369..21793)
                     /gene="lpdA"
                     /locus_tag="OQM47_000586"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1478865.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22118..23998)
                     /gene="aceF"
                     /locus_tag="OQM47_000587"
     CDS             complement(22118..23998)
                     /gene="aceF"
                     /locus_tag="OQM47_000587"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1478866.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24013..26676)
                     /gene="aceE"
                     /locus_tag="OQM47_000588"
     CDS             complement(24013..26676)
                     /gene="aceE"
                     /locus_tag="OQM47_000588"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1478867.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26837..27601)
                     /gene="pdhR"
                     /locus_tag="OQM47_000589"
     CDS             complement(26837..27601)
                     /gene="pdhR"
                     /locus_tag="OQM47_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1478868.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28142..29515
                     /gene="aroP"
                     /locus_tag="OQM47_000590"
     CDS             28142..29515
                     /gene="aroP"
                     /locus_tag="OQM47_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1478869.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29558..30412)
                     /gene="ampE"
                     /locus_tag="OQM47_000591"
     CDS             complement(29558..30412)
                     /gene="ampE"
                     /locus_tag="OQM47_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1478870.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30409..30960)
                     /gene="ampD"
                     /locus_tag="OQM47_000592"
     CDS             complement(30409..30960)
                     /gene="ampD"
                     /locus_tag="OQM47_000592"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1478871.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31048..31941
                     /gene="nadC"
                     /locus_tag="OQM47_000593"
     CDS             31048..31941
                     /gene="nadC"
                     /locus_tag="OQM47_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1478872.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32144..32584
                     /gene="ppdD"
                     /locus_tag="OQM47_000594"
     CDS             32144..32584
                     /gene="ppdD"
                     /locus_tag="OQM47_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1478873.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32594..33741
                     /gene="tadA"
                     /locus_tag="OQM47_000595"
                     /pseudo
     CDS             32594..33741
                     /gene="tadA"
                     /locus_tag="OQM47_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33731..34933
                     /gene="hofC"
                     /locus_tag="OQM47_000596"
     CDS             33731..34933
                     /gene="hofC"
                     /locus_tag="OQM47_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1478874.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(34968..36011)
                     /gene="guaC"
                     /locus_tag="OQM47_000597"
     CDS             complement(34968..36011)
                     /gene="guaC"
                     /locus_tag="OQM47_000597"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1478875.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36173..36211)
                     /locus_tag="OQM47_000598"
     CDS             complement(36173..36211)
                     /locus_tag="OQM47_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478876.1"
                     /translation="MIIKTLKMSARK"
     gene            36236..36856
                     /gene="coaE"
                     /locus_tag="OQM47_000599"
     CDS             36236..36856
                     /gene="coaE"
                     /locus_tag="OQM47_000599"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1478877.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36856..37599
                     /gene="zapD"
                     /locus_tag="OQM47_000600"
     CDS             36856..37599
                     /gene="zapD"
                     /locus_tag="OQM47_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1478878.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37609..37806
                     /gene="yacG"
                     /locus_tag="OQM47_000601"
     CDS             37609..37806
                     /gene="yacG"
                     /locus_tag="OQM47_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1478879.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37906..38295)
                     /gene="mutT"
                     /locus_tag="OQM47_000602"
     CDS             complement(37906..38295)
                     /gene="mutT"
                     /locus_tag="OQM47_000602"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1478880.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38355..41060)
                     /gene="secA"
                     /locus_tag="OQM47_000603"
     CDS             complement(38355..41060)
                     /gene="secA"
                     /locus_tag="OQM47_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1478881.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41122..41634)
                     /gene="secM"
                     /locus_tag="OQM47_000604"
     CDS             complement(41122..41634)
                     /gene="secM"
                     /locus_tag="OQM47_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1478882.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41865..42782)
                     /gene="lpxC"
                     /locus_tag="OQM47_000605"
     CDS             complement(41865..42782)
                     /gene="lpxC"
                     /locus_tag="OQM47_000605"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1478883.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(42883..44034)
                     /gene="ftsZ"
                     /locus_tag="OQM47_000606"
     CDS             complement(42883..44034)
                     /gene="ftsZ"
                     /locus_tag="OQM47_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1478884.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44095..45357)
                     /gene="ftsA"
                     /locus_tag="OQM47_000607"
     CDS             complement(44095..45357)
                     /gene="ftsA"
                     /locus_tag="OQM47_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1478885.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45354..46184)
                     /gene="ftsQ"
                     /locus_tag="OQM47_000608"
     CDS             complement(45354..46184)
                     /gene="ftsQ"
                     /locus_tag="OQM47_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1478886.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46186..47106)
                     /gene="ddlB"
                     /locus_tag="OQM47_000609"
     CDS             complement(46186..47106)
                     /gene="ddlB"
                     /locus_tag="OQM47_000609"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1478887.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47099..48574)
                     /gene="murC"
                     /locus_tag="OQM47_000610"
     CDS             complement(47099..48574)
                     /gene="murC"
                     /locus_tag="OQM47_000610"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1478888.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48628..49695)
                     /gene="murG"
                     /locus_tag="OQM47_000611"
     CDS             complement(48628..49695)
                     /gene="murG"
                     /locus_tag="OQM47_000611"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1478889.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49692..50936)
                     /gene="ftsW"
                     /locus_tag="OQM47_000612"
     CDS             complement(49692..50936)
                     /gene="ftsW"
                     /locus_tag="OQM47_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1478890.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(50936..52252)
                     /gene="murD"
                     /locus_tag="OQM47_000613"
     CDS             complement(50936..52252)
                     /gene="murD"
                     /locus_tag="OQM47_000613"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1478891.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52255..53337)
                     /gene="mraY"
                     /locus_tag="OQM47_000614"
     CDS             complement(52255..53337)
                     /gene="mraY"
                     /locus_tag="OQM47_000614"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1478892.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53331..54689)
                     /gene="murF"
                     /locus_tag="OQM47_000615"
     CDS             complement(53331..54689)
                     /gene="murF"
                     /locus_tag="OQM47_000615"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1478893.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54686..56173)
                     /gene="murE"
                     /locus_tag="OQM47_000616"
     CDS             complement(54686..56173)
                     /gene="murE"
                     /locus_tag="OQM47_000616"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1478894.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56160..57926)
                     /gene="ftsI"
                     /locus_tag="OQM47_000617"
     CDS             complement(56160..57926)
                     /gene="ftsI"
                     /locus_tag="OQM47_000617"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1478895.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(57942..58307)
                     /gene="ftsL"
                     /locus_tag="OQM47_000618"
     CDS             complement(57942..58307)
                     /gene="ftsL"
                     /locus_tag="OQM47_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1478896.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58304..59245)
                     /gene="rsmH"
                     /locus_tag="OQM47_000619"
     CDS             complement(58304..59245)
                     /gene="rsmH"
                     /locus_tag="OQM47_000619"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1478897.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59247..59705)
                     /gene="mraZ"
                     /locus_tag="OQM47_000620"
     CDS             complement(59247..59705)
                     /gene="mraZ"
                     /locus_tag="OQM47_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1478898.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14406 a        16702 c        14810 g        14316 t
ORIGIN      
        1 gatacaacag gaggagttta cccggatcaa gatacaggta taacataccc tctccatttc
       61 caggccacgc tacaacagga tggaactata ccaatagaag ctggtgagtt taaagccacc
      121 agtactttcc aggtaaccta cccttaataa gtccaccgca ccgccatcat ctggcggtgc
      181 ggtaattgat aaatcattgc ccccaccaat gtgactgata aaatgccagg caacacgaca
      241 tcatttatca ggatacgtta tgaaaccgac caccattgct tcacttcaga aatataagca
      301 ggataaaaag cgtttcgcga ccatcaccgc ttacgactac agcttcgcga aactatttgc
      361 tgatgaaggg cttaacgtca tgctggtagg cgactcgctg ggcatgaccg ttcagggcca
      421 cgattccact ctgccagtaa ccgttgctga tatcgcctac cacactgccg ccgtacgtcg
      481 cggcgcacca aactgcctgc tattggctga cctgccgttt atggcgtatg ccacgccgga
      541 acaagccttt gaaaacgccg caacggttat gcgtgccggt gccaatatgg tcaaaattga
      601 gggcggtgag tggctggtcg aaaccgtaaa aatgctgacc gaacgtgccg ttcctgtatg
      661 tggtcactta ggtttaaccc cacagtcagt gaatattttc ggtggctaca aagtacaggg
      721 gcgcggcaat gaagcgagcg atcgactact cagcgatgca ttagccttag aagccgcggg
      781 ggcacagctg ctggtgctgg aatgcgtgcc ggttgagctg gcaaaacgta ttaccgaagc
      841 actggcgatc ccggttattg gtattggtgc aggcaacgtc actgacgggc aaatcctcgt
      901 gatgcacgac gcctttggta ttaccggcgg tcacattcct aaattcgcta aaaatttcct
      961 cgccgaaacg ggcgacatcc gcgcggctgt gcggcagtat atggctgaag tggagtccgg
     1021 cgtttatccg ggcgaagaac acagtttcca ttaaggagtc acgttgtgtt aattatcgaa
     1081 accctgccgc tgctgcgtca gcaaattcgc cgcctgcgta tggaaggcaa gcgcgtggcg
     1141 ctggtgccta ctatgggtaa tctgcacaat ggccatatga agctggtcga cgaagccaaa
     1201 gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc cgatgcagtt cgaccgcccg
     1261 gaagatctgg cacgttatcc acggaccttg caggaggact gcgagaagct aaacaaacgt
     1321 aaagtggatt tagttttcgc cccttcggta aaagagatct acccgaacgg tactgaaacc
     1381 cacacttacg ttgacgttcc tggcctttcg accatgctgg aaggtgccag ccgtccggga
     1441 cattttcgcg gcgtttcgac tattgtcagc aagctgttca acctggtcca gccggacatc
     1501 gcctgcttcg gtgaaaaaga ttttcagcaa ctggcgttga tccgcaaaat ggttgccgat
     1561 atgggcttcg atattgagat tgtcggtgtg ccaattatgc gcgccaaaga cggtctggcg
     1621 ctaagttccc gtaacggtta tctgacggcg gaacaacgca aaattgcgcc tggtctgtac
     1681 aaagttttaa gttcgattgc tgacaaattg caggctgggg aacgggatct cgatgaaatt
     1741 attaccattg cggggcaaga actgaatgaa aaaggcttcc gcgccgatga tattcagatt
     1801 cgcgatgccg acacattgct ggaagtttct gaaaccagca aacgggcagt aattctggta
     1861 gccgcctggc ttggcgatgc tcgcctgatc gacaacaaaa tggtcgagct ggcgtaatac
     1921 ttaactggcg ctacggctga tggcgccagt tattaattta ccccacgtca tctgtcataa
     1981 cacaggtaac ttaaataagg ttaattacct tatcaatctc agcaagaggt aaatttgcca
     2041 tctctgcaac gtcttcccga gacattcctt tacttagaag acgcagggcg aattcttgcc
     2101 tttcctcctg tcttccctgc tgaatcccct tctcaatccc tttctcttca aaccactgcg
     2161 ccagcgtcat catagacttc cctcccgttt ccctgtctct cagcacacca taaaacaaat
     2221 ccgcttgttc agtatgaccg cgttgcagca tatagttttg catggcaact aactgacttc
     2281 cgctagtgta cccttcgtca atcagcgtga ccagttgctc cagcaataac attaagtcgc
     2341 gctggcgaat atgtttttgc agtagttcga gaatcgcaat ccgccgatgt tgcatgattt
     2401 cgtcatccgg tgtgatggtg atatccacca gcgggaaagg actgttatag acgcgtcgcg
     2461 ccagctccgg cgagtaaaac atatcaaacc agcacattga gagcggataa ggtgtggcct
     2521 cgccctgata aaacagtatc ggcaccacca gcggcagctt atcgtggcca gcctccagat
     2581 gccggtgcat ggcagcaata gaataacgca tcatgcgaaa ggccattttc ttatccggct
     2641 tgctttggtg ttcaatcaca acatgcagat aaccgggatt gccctgcatt tgcatggaat
     2701 agagcacgtc cgtgctgtga cctttcaggc actcttcaat gaaactcccc gactctaaat
     2761 gaagcgtgtt gagatcacaa agttcgcgta attccactgg caaatgtatc tccagaaagt
     2821 cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac cgcgtcatgc ggtgtggtac
     2881 tcggtgcatc catcttccct gtcttccgtg tggccttgcg tgaaggcagt atccagtcaa
     2941 ggcgtgctat cgtcagccac ctctttattg tttaccgagc agcgttcata aaaatatcaa
     3001 ttcgttacag gcgttacatg gcacgcttcg gcgcggtacg caaactgctg acgcaaatgc
     3061 catcagatga gtagacactc atcaaaaatc gggcaatact gcgtgagaat ttcctcaaag
     3121 caggcaccgc ctgcttcgtt aacgacaggg tagaaaggta gaagttatga ttcgcacgat
     3181 gctgcagggc aaactccacc gcgtgaaagt gactcatgcg gacctgcact atgaaggttc
     3241 ttgcgccatt gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg aagccatcga
     3301 tatctggaat gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg
     3361 ttcgagaatt atttctgtta acggtgcggc ggcccactgc gccagcgtcg gcgatattgt
     3421 catcatcgcc agcttcgtta ccatgccaga tgaagaagct cgcacctggc gacccaatgt
     3481 cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc cggtacaggt
     3541 tgcttgattg ttatccctgc ggctggttac tcaccagccg cgacatcttc tccagcgaat
     3601 ccgttcttaa gatataaagt cgttttagta acaacggatt atcccccggt tttactttgc
     3661 ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc acggctttgt
     3721 cattaaagcc gccaaacgga tacgaaagat accagacatg tggattaaat tgcgccagag
     3781 cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc actacggctc agtaatatgg
     3841 gtcggcgata accatctacc cgatgcaaaa aatgggtatg tgactggaaa tcaaaaacat
     3901 cgcgaatttc gttaagctca gaaacgctca taaattgcag cgattttggg ttccacttct
     3961 gcgggtgacg tttgatgcgt gaggtaacaa taaacaccgt cgccttcatg ccatattgtt
     4021 tcaacacagg atacgcatag cggctcaccg acttgaggcc atcatcaaag gtaatcacca
     4081 ccgctcgcgc agggagattg atcgtattct tcacgtaacc ttccagctga gccatgctca
     4141 gtgtcgcgta tcccctgtcg cgcagccagg ccatttggtt attgaaagcg cgtaccgagg
     4201 tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc gcgcagaata tggtgataag
     4261 ttagcaccgg caggccatta tcgggttggg catccagcgc gctgatatag gccagtcgat
     4321 cgccaatacg gatctgatac caggtttgat ttaacctgtc tttcagttta tgcaaaatcg
     4381 ggtagcgcaa attgtccgcc agtaccccaa atggcgcact gcccacactc ggcgcgttgt
     4441 agaccggcgt atctttccag gtaattaagt tctgattact cagcggcttg ttgaggtcac
     4501 ccaaaccgtc ttcaactttt tgtcgtccct gaactggctc gagatgccct ttatcgataa
     4561 accctttacc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa
     4621 tgatttgtcc ggcccgaata ttccccacgg tcaccatctt gtcactaatt tgcgcccaga
     4681 ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcactg acacttgcgg
     4741 taaacagcat cagcaggaga ataacagctt gtttatacat gaaaaaataa ccgagggtga
     4801 aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc gggttaatac tcatacaaat
     4861 tatgtttgag gacgaaaggc ggattttttt gttgttggtg ccaaagacta ctcacctccg
     4921 gcccacccag ttcgacaata cactcacgaa actctgaact ggtcatggtt tcacggcaag
     4981 ccatcatctg ttcaattaac ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt
     5041 tgtggcttaa taatgaagcc acgcgatacg gtggcgctcc ggctatatcg gttaagaaga
     5101 tgacaccctc accagagtcc gcttcatgca gagcatcgca catcatgcgg ctgagcatat
     5161 tagagcttaa tccgcgccag aaattcacgg cccggcactg aagaagtgcc ccatgttttt
     5221 tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta
     5281 acatcgcctt cctccttagc cgaaaatcag cctaccctga ccattgttga taaagggtga
     5341 tagccgtcaa aactcctctc ccccgaatcc agaggagagg aaatagcctt agctacgcaa
     5401 accacgtcca cgttggatca gcgaccaaca gatcaaataa aacgccacaa taaagaccac
     5461 cagtacgcca aaggtagtga ccagcggcac atcatttata ccgaggaagc cgtagcggaa
     5521 accactgatc atataaacga ttgggttcag gtgcgacagc ccttgccaga acggcggcaa
     5581 caaagtcagt gagtaaaaga ccccgcccaa atacgtgagt ggcgttaaca caaaggttgg
     5641 caccaggctg atgtcatcga acgttttggc aaacacgccg ttcagcaaac ccgcaaggga
     5701 gaatagcacc gccgtgagca ccagggttaa ggcaacgaat acccacgaat gcacctgaaa
     5761 cggcacaaaa aacagtgaaa ttgccgtcac aagaatgcca acaaacagac cacgcgccac
     5821 gccaccgccg acatatccgg caataatgac gtgagtcgga accggcgcta ccagcagctc
     5881 ttcaatatta cgctggaact tggcaccaaa aaatgatgac gcgacgttag cgtaggcgtt
     5941 ggtgatcacc gacatcatga tcagccccgg tacgatgaac tgcatatagc tgaagccatg
     6001 catatcgcca atacgcgaac ctatcaggtt accgaagata ataaagtaaa gggtcatggt
     6061 gatgactggc ggcaccagcg tctgcaccca gatacgcata aagcgatgga tctctttcgc
     6121 ccagatgctt tttagcgcca cccagtaaag atgcatcatg cgcgatctcc ttgtttttca
     6181 ttaaccagtg aaacaaacag ctcttccaga cggttagctt tgttacgcat acttaatacc
     6241 tgaatgccct gctcacttaa ctgcgtaaat acgctgttga tcccctgctc acgtagcact
     6301 tcaacttcca gcgtcgcggt atcgaccagt cgatactgat agccatcgag cttcggtaac
     6361 ggacttttcg gtgcgagatc gagaataaag gtttccgatt tcagcttcgc cagcagcgcc
     6421 ttcatcgagg tattttccac cagctcacca tgttgaataa tgccgatatt gcggcacagc
     6481 atttctgctt cttccaggta gtgtgtggta agaatgatgg tggtgccttt gtcgtttaaa
     6541 tccttcaaaa agccccacat tgagcggcga agttcaatat ccacgcctgc ggtcggttcg
     6601 tcgagaatca gtagtttagg ttcatgcatt aacgcacggg caatcattaa acggcgcttc
     6661 atcccgccag ataacatacg cgcacgttcg ttgcgttttc cccataaatc gagctgctta
     6721 agatattttt cgctgcggat gtacgcctct ttgcgctcca caccgtagta ccctgcctga
     6781 ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt taaattcctg cggcaccagt
     6841 cccaactgac gtttagcgtt cacgacatct ttctcgagat cgtaaccaaa tacgctgacc
     6901 cgcccggagg ttttatttac cagagagctg ataataccga tagtggtcga tttcccggcc
     6961 ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc tattccacga
     7021 agcgcctgaa cgccgcctgg atagtttttt ttaagctgtt gaagttccag tgcaatggtc
     7081 ataaattttt acttacctta cgttcttaca ctttatctgt ggtttaaatc gtcccggagt
     7141 tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc atgaaagaca
     7201 tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa gaggatcccg
     7261 ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt ggatgttccg
     7321 acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc
     7381 gtaatgttgc taacctagtc attcacaccg acctgaactg cctttccgtg gttcagtatg
     7441 cagtggatgt actcgaagtt gaacacatta ttatctgtgg ccactacggt tgcggcggcg
     7501 tacaagccgc cgttgaaaac ccggaactgg ggcttatcaa caactggctg ctgcatatcc
     7561 gcgatatctg gttcaaacat agctcattgc tcggcgaaat gccgcaagag cgccgtctgg
     7621 ataccttgtg tgaactgaac gtcatggaac aggtgtataa cctgggccac tccaccatta
     7681 tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggcc tacggcattc
     7741 acgacggctt gctgcgtgat ctggatgtta ccgccaccaa ccgcgaaacc cttgagcaac
     7801 gttaccgtca cgggatttcc aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc
     7861 atgccggatg caacacatcc ggcaacttca cacttactcg tccagcagaa tcactttgcc
     7921 aatatacggc agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc
     7981 atccgggatc gagaaaccga taaattcaac cgggacgttc acttcacgac gggacggttt
     8041 atccagcagc gtacaaatcg ccaacgactt cggttcgcgc aggcttaaga tctcacgcac
     8101 tttcgacagt gtattccccg agtcgatgat atcttcaaca atcagcacgt ccttgccacg
     8161 gatatcttca tccagatctt tgaggatttt cacatcacgg gtggtggaca tgccgctacc
     8221 gtagctggag gcggtcataa agtcgacttc atgagatacc tgaacttcac ggcacaggtc
     8281 cgccataaac ataaatgagc cacgcagcag acccaccagc accatatcgc tgccgctgtc
     8341 tttgtaacgc tcagtaatct gacgacccag ttcggcgata cgcgctttaa tctccgcttc
     8401 ggggatcatt acttctacag tatgtttcat atctctaacc atatgattta aaaacaaatc
     8461 acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa gacgcgcagt ataccagtaa
     8521 aactattcat tcgcggaatc tgttaataaa gctcgaattg tgatgacggt cacacatgtt
     8581 aaaacctata attaattgct actaaaatat taatgaattg aaatggtgtc tctttatggc
     8641 aattaacaat acagcctcgc gacgattact cgtcacgcta acagcccttt ttgcagcgct
     8701 ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg gctcctggta
     8761 ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg atgctgtggc gcagtaaacg
     8821 cgccgcgctt tggctatacg cagccctgct gctcggcacc atgatttggg gcgtctggga
     8881 agttggtttc gacttctggg cgctgactcc gcgcagcgat attctggtct tcttcggcat
     8941 ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc
     9001 cgcactggtg gtcgcactgc tgattagcgg tggtatcctg acctgggccg gatttaacga
     9061 tccgcaggag atcaacggca ccttaagcgc cgatgccaca cctgccgaag ctatctcccc
     9121 cgttgccgat caggactggc ctgcgtatgg ccgcaatcag gaaggtcaac gcttttcacc
     9181 actgaagcaa attcacgccg ataacgtcca caagctgaaa gaagcctggg tgttccgtac
     9241 tggcgatgtg aagcagccga acgatccggg tgaaatcacc aatgaagtga cgccgattaa
     9301 agtgggcgat accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc ttgatgccgc
     9361 cagcggcaaa gagaaatggc attacgatcc tgagctgaaa accaacgagt ctttccagca
     9421 cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt cgccggaagt
     9481 gatggcggat tgcccgcgtc gtatcattct tccagtgaat gatggtcgcc tgattgcgat
     9541 taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat aaaggcgtgc tcaatctgca
     9601 aagcaatatg ccagacacca aaccgggtct gtatgagccg acttctccgc caattatcac
     9661 cgataaaacc atcgtgatgg ccggttcagt caccgataac ttctcaaccc gcgaaacgtc
     9721 tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt ttgatccggg
     9781 cgcgaaagat ccgaacgcaa tcccgtcaga cgaacacacc tttaccttta actcaccaaa
     9841 ctcgtgggca ccagcggcct atgacgcgaa gctggatctg gtctatctgc cgatgggcgt
     9901 gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt atgccagctc
     9961 gattctggcg ctgaatgcca ctaccgggaa actggcgtgg agctaccaga ccgttcacca
    10021 cgacctgtgg gacatggatc ttccggcaca gccgacgctg gcggacatca ccgttaatgg
    10081 tcagaaagtg ccgattattt acgctccggc gaaaaccggt aacatttttg tgctcgatcg
    10141 tcgtaatggc gaactggtgg ttccggcccc ggaaaaaccg gttccccaag gtgctgccaa
    10201 aggcgattac gttaccccta ctcaaccatt ctcggaactg agcttccgtc cgacgaaaga
    10261 tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt gccgcgtgat
    10321 gttccaccag atgcgctatg aaggcatttt cacgccgcca tctgaacagg gtacgctggt
    10381 cttcccgagt aacctgggga tgttcgaatg gggcgggatt tccgttgatc caaatcgtga
    10441 agtggcaatt gccaacccaa tggcactgcc gtttgtttcg aaactgctcc cgcgtggtcc
    10501 tggcaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat ccggcattca
    10561 gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac catttggtct
    10621 gccatgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa ctaatgaagt
    10681 ggtgtggaag aaacgtattg gtacgccgca ggacagtatg ccgttcccga tgccggttcc
    10741 ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg cgggtaacgt
    10801 gctgtttatc gccgctacgg cagataacta cctgcgcgct tacaacatga gcaacggtga
    10861 aaaactgtgg cagggtcgtt taccggcggg tggtcaggca acaccgatga cctatgaagt
    10921 gaatggcaag cagtacgtgg tgatctccgc aggcggtcac ggttcatttg gtacgaagat
    10981 gggcgactat attgtggctt atgccctgcc ggatgatgtg aaataaccat aatgcccgga
    11041 gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa catcatcccc
    11101 gtatcttcat gctccagcag atggcagtgc gccatataag catgttcttt cggtgcgttg
    11161 tgattaaact tcaccagcac ttcactgaca ttgccttcta ccttaacggt atctttccag
    11221 cccgcgcgat gagccgctgg cggtttgcca ttttctgaca agatacggaa ctgcgtgccg
    11281 tgaatatgga acggatgcag catcatgtcg cccacgccag agataaccca acgttcgtac
    11341 tgccctttcg ccgccgcaaa catcggctta ttcatatcaa acgcctgacc gttgatttta
    11401 ttggcatggt ggaaatcgaa cttcccgccg tggttcatat gattcatatt gccgtgcccc
    11461 atatggccca tcatctggct gtgatccatt ccggccatcg cctgatcgcc atatttctcc
    11521 attagcatct gcatccccat catatcgagc atcgggtcca tagaaagttg cagcttgcgt
    11581 accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc aggcaaagca
    11641 ccggaggcac taatagcaat cggctgaatc cgcattaccg gatgcggctt gtcaaacggt
    11701 gcaatcgcca tccccatctg gctgaccggc agcgtcacca ggtcaaaggg tttgttgtcg
    11761 ttaacctcca ccagcacttc aaaacgctcg cccatcagca ccggcagctc gctcaccttc
    11821 accggttcag gtagcagacc accgtcgctg gcaatcacat acagcgggcg attgtcgctg
    11881 gtggcgaaat tgagcgagcg ggcattacag ccattgagca aacgcaggcg cagccaacca
    11941 cgcggggcag cgtgttgtgg gtagattgca ccgttggtca gcagcgtatc gccaaaccag
    12001 cccacggcgg cggtcatcac atccagttga taatcaatct gcccgtcggc gctaaatttc
    12061 ttatcctgaa cgatcaccgg aacatcatcg ataccccact gttttggcag cattaatttc
    12121 aggatctcgt catcttcaat caccaccaga ccagccagcc ccatcgccac ctgtcgcccg
    12181 gtcttgccat gttgatgtgg atggaaccag caggtagcgg caggttgatc aacgttcaac
    12241 gtcaccgagc gcttgccacc tggcggaata attccctgtg ggccgccgtc gacgtcaccc
    12301 ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg ccagttggtt gtagatatca
    12361 accgttaccg ctttgccgcg ctgtaatttc accgccggcc ccagcagatt gccgttatag
    12421 ccccaggtag ttgcagtttt ctcgccaaag gtggactgac ctgcgccaat agttaactga
    12481 atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg gcgttccgcc
    12541 gcaaagactg cacggctcca cagtggcaag gctgaagcca cacccagtgc gacggaatat
    12601 ttcaagaaat cacgacgttg catagttatt tccttattct taagcaggcg aaacaaaatc
    12661 aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac aatttaatcg
    12721 acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg
    12781 tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatc gccgtttgcg
    12841 caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta acggcagttt
    12901 ttgtctttct gaagaacgat tgtggttacc agaacttatc taacgggcaa attcgtcgcg
    12961 cactggtctt tttcgcccag caaaaccagt gggatctcag taattacgac accttcgaca
    13021 tgaaagctct cggtgaagac agctaccgcg atctcagcgg cattggcatt cccgtcgcta
    13081 aaaaatgcaa agccctggcc cgcgattcct taagcctgct tgcctacgtc aaataatccc
    13141 tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc tattatgttg
    13201 cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa aaaaaacagt
    13261 ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac gttctgtatc
    13321 atgaaaagac cgatcaccag gatctgatca tttttgagaa cgctgcattt ggtcgcgtaa
    13381 tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat catgagatga
    13441 tgacccatgt tccgctactg gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg
    13501 gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca atcacgatgg
    13561 tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta tctgcccaac cataacgccg
    13621 gtagctacga cgatccgcgc tttcaactag tgatcgacga tggcgtcaat ttcgttaatc
    13681 aaaccagcca gacctttgat gtcattatct ccgactgcac cgatcctatc ggtcccggcg
    13741 aaagcctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat cctggcggta
    13801 tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaagccatc gacagccatc
    13861 gcaaactcag ccattacttc agcgacgttg gcttttatca ggcggcgatc ccgacctatt
    13921 acggcggtat catgactttt gcatgggcga cagataacga cgccttacgc catctctcaa
    13981 ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat tacaatccgg
    14041 caatccatac ggcagctttt gccttacctc agtatctgca agacgcactg gcttcacagc
    14101 cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt aataatctga
    14161 ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactgcc gaagagcgcg
    14221 acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc gaaatcctgt
    14281 cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgccag gattacgaac
    14341 cacagggtgc cagcgtcact attctggtga gtgaagaacc ggttgacccg aaactcatcg
    14401 acaaaacaga acaccccggc ccactgccag aaacggtggt tgctcatctc gataaaagtc
    14461 atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt accttccgcg
    14521 ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg aattacctga
    14581 tccaccagct tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt tttactcgcg
    14641 acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag aactttatgt
    14701 ctgacgatat gaaggcgcgg tatgacatgg tggatgtaaa cgtctatcag gaaaatatct
    14761 tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc cacaccaaac
    14821 cggaagattt aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg aaagaaatgc
    14881 gcgagattta ttacgggcgc aatatgccag ctgtttaacg gctctggcgg agctcccagg
    14941 ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca cgacctgcag
    15001 aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc
    15061 catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt cagcgtccat
    15121 ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt tcagtgcgtg
    15181 cgctgcttgt tccacttcat caagcaaggc caggttttct ttcacctctt cattaaacca
    15241 gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg
    15301 cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcatcg
    15361 cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg cgatagttta
    15421 gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    15481 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    15541 agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    15601 aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc
    15661 cagttctgca gaagccagga agacattcgc gccagtaccc aaacggttcg ggaagttacg
    15721 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    15781 ggaacagccc gggatctcga tacgagcccc gctcttaccg aagacgctgt agtagccttc
    15841 ttccgtcagt tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcaa
    15901 ctgacctttg tgcgcatcca gcagtttacc agcggcacgg aagtgaccga tgttagtcat
    15961 gcaagaaccg ataaatactt cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc
    16021 gtcatccgga tcgttcggag cacacaggat tggctcttta atctccgcca gatcgatgtc
    16081 gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca
    16141 tttttccatg ccctgaatac ggcgttccag ggtacgacga tcgccgtaac cttccgcgat
    16201 catccacttc agcaggacga tgttggagct caggtattcg atgatcggtt ctttgttcag
    16261 cttgatagta caaccagcgg cagaacgctc ggcggacgca tcggttagct caaaggcctg
    16321 ctcaactttc agatccggca gaccttcaat ttccagaatg cgaccagaga agatgttttt
    16381 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcgtg
    16441 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    16501 ttccggcata tccagcggca ttacgccagt tgcggcagca aacgccacca gaccagaacc
    16561 cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg taccgacggt
    16621 atccggcagc agcatacggt tcagccagga gtgaatgacg ccgtcacccg gacgcagcga
    16681 cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    16741 cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    16801 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gagagcctac
    16861 agaagtcatt ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg
    16921 gcctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    16981 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    17041 cccgataatc agcggaatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt
    17101 cagttcgaag gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg
    17161 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg ttgacttcga ttggcagtgc
    17221 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    17281 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    17341 cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    17401 acctttctgt tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg
    17461 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    17521 tgaccacgca tccggtgccg gagaaaggtc atcggtgttg gtttcgccag tgactttaaa
    17581 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    17641 ccaggactgc attacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc
    17701 atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa taggtgccag
    17761 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc
    17821 cagcagttcg atagcttttt ccggagtcag cagaggggat ttagcttcgc ctttagcgat
    17881 agcagccagg aagcctgctt ttacataggc ggcttcatcg acgcctgggg gaacacggtt
    17941 ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    18001 aagcgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    18061 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    18121 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    18181 accgcaacgg gcagaatagc aggattttga cgacgtgtta atctgtttac aaaaaagcaa
    18241 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    18301 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    18361 aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat
    18421 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    18481 gcgctaattt gcagtgccgg gctttgtgct gcctctgccg ggctatatat aaaaagccgc
    18541 acagtggaag cgcctgtaga accgcaatcg acacaactgg ctgtgtctga cgctgccgct
    18601 gttacgcttc ctgcaacggt ttccgcacct cccgtaatac ccgcagtcgt caaatccgca
    18661 ttcagcactg cacaaataga tcaatgggtc gcacccgtcg cgctgtatcc cgacgcccta
    18721 ctttcgcagg tgctgatggc atcaacctat ccgacaaacg ttgctcaagc agtgcaatgg
    18781 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    18841 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    18901 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    18961 gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc
    19021 gaacagaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    19081 cccgtcatac catccaatac cgttttaact gccaaccccg tcattacaga gccggcaaca
    19141 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    19201 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    19261 gccggagaac cgtttgttga cagctttgtg cgcggattcg gctatagcat gggcgttgct
    19321 accacgtacg cactattcag cagcatcgac tgagatgacg acgatcatga ccatcatcat
    19381 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacagtaa tggctggcaa
    19441 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    19501 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    19561 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc
    19621 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    19681 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    19741 cggtttaatg aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    19801 caaccactga cccagcagca aaaggacgcc gctcgtcagc gttatcagtc ggcctctcct
    19861 gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cgcagatcca gcagcgaagg
    19921 gaggcggcgc gccagcgtat tcagtccgcc tcgcctgagc agcgccaggc ggttcgcgag
    19981 aaaatgcaga ctaacccgca gaaccagcag cgaagagagg cagcgcgtga gcgtattcag
    20041 tctgcaactc ccgaacagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac
    20101 caacagcaac gtgataacgc ccgccagcgt atacaatcgg catcacctga acaacgtcag
    20161 gtttttcggg agaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact
    20221 gtcaggctga ataacgagca acggtcagca gtacgcgaac gtctctctga acgtggagca
    20281 agaagactgg aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
    20341 attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt
    20401 cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt
    20461 gcgcgtggat ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccaggccga
    20521 tttcacccag cagctcgccg ccgttggtac caacaatcgc accaccgatc acacggtgag
    20581 attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcagaagcg atagcacgac
    20641 cagaagcagc ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt
    20701 tctcagtcag acctacccat gcaacttctg gttcggtata ggcgatggac gggataactt
    20761 tcggatcgaa gtagtgtttc ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa
    20821 cacctttgtg tgccagcatc ggctgaccga cgatatcgcc gatagcaaag atgtgcggta
    20881 cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtcaact tccacgccag
    20941 ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt
    21001 cgtaacgctg cggttcagct ggtgcttttt tgccttccat cgtcacataa ataccgtctt
    21061 ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac
    21121 gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt
    21181 caaccacgtc aatctgtgaa cccagcgcgt ggtaaacggt gcccatttcc agaccgatga
    21241 taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg
    21301 agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg
    21361 ctgcaatgat cgcattgtcg aagttgatca cggttttgcc gttctcacct tcaacttcca
    21421 gggtgttagc cccggtgaat ttacccagac cgttgaccac tttgactttg cggcctttcg
    21481 ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct
    21541 tgtcaatatc ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt
    21601 cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc
    21661 aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc
    21721 aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga
    21781 tttcagtact catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc
    21841 aggccgcagc ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg
    21901 gggattctgg cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata
    21961 ccctaaccac caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt
    22021 aaacaacaaa cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt
    22081 accagaaaaa agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt
    22141 cagacagcgt gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc
    22201 ggtggtcgaa ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc
    22261 acaccggctc catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa
    22321 tcggcgcgaa gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct
    22381 gcatttcgcc cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc
    22441 gagacagctc gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt
    22501 tcggggtatc caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt
    22561 cttccgacag cgaactattg aagcgaggca tctgctcaag agctgcagca acggctttca
    22621 tgatgaagac aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct
    22681 gctgtttacg gaacgcttcc agctcggtga tatcggtttt gtcgaagtgg gtaacatgcg
    22741 ggatcatcac ccagttacgg ctcaggttag caccagaaat tttctggata cggcccagtt
    22801 ccacttcttc gatttcgcca aacttgctga agtccacctt cggccacggc agcatgccag
    22861 ggataccacc gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt
    22921 aagcctgaac gtcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca
    22981 ggttaacgcc aaactcgcgt gccagacggc ggatcagcgg ggtcgcgtga acgtaagcgt
    23041 cgttttcagc aaattcagat ttgccttccg ctttcgcagc cggagcttca gcttttgctg
    23101 ccggagccgg cgctgccgct tcctgtttcg caggagctgc cgcaggcgct gcgccttcaa
    23161 cttcgaagat cataatcagc gaaccagttt tcactttatc gccaacgttg actttcagtt
    23221 ccttcacgac gcctgcaaac ggagccggaa cttccataga agctttatcg ccttctacgg
    23281 taatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt
    23341 caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagct gctggtgcag
    23401 gggtcgctgc cggagtcgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg
    23461 cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg ttcactttga
    23521 tctctttcac ggtacctgca aacggagccg gaacttccat agaagccttg tcgccttcta
    23581 cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg atttcggtca
    23641 cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgcc gcagccgcgg
    23701 gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg
    23761 caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta tcgccaacag
    23821 agactttgat ctctttaacg atacccgcct gcggagatgg aacttccata gaggctttgt
    23881 cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga
    23941 tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta
    24001 ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc
    24061 gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc
    24121 cgcaaccacg acataagaag catcaacttc gaagtggtga cgcaggttct cacggctgtc
    24181 ggaacgaccg aagccatcag tacccagtac gcgatagtcg tcagccggta cgtaagtacg
    24241 gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggcg cgtcgttcat
    24301 cacctgagcg atatacggaa cgcgcggagt ttccagcgga tgcagcatgt tccagcgttc
    24361 acaatcctga ccatcacgcg ccagctcggt gaaggaggtc acgctataaa cgtcagaacc
    24421 tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatggaacc
    24481 ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc
    24541 tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag
    24601 cgtagtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagaccgtc
    24661 atgcataatg acagcaactt cgtaagcgta agccgggtcg taagagatac agttcgggat
    24721 agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt
    24781 ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtctc cagccgccca
    24841 gcacagatcg ccgatacgct ggaagccgaa catcgagtaa tagatgtaga atgggatcat
    24901 cggcagattg ttcgtgctgt aagaggtcgc cgctgccagc caggaacacc ctgcgcccag
    24961 ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg
    25021 ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa tctgacggaa
    25081 cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt
    25141 gatcgacttg ttcttcagca tcacgttcag agcacgaacg aaagcgatag tggtagagag
    25201 ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt
    25261 ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac gctgagcgtg
    25321 cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc
    25381 tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat
    25441 tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt
    25501 aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc
    25561 agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt
    25621 ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt cacgaacgta
    25681 cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg
    25741 gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac
    25801 tttgatcacg ttccagccag caccttcgaa gatgccttcc agttcgttga tgatcttgcc
    25861 gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga aaaccaggtt
    25921 atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc
    25981 accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc cacggtgttc
    26041 cagatatttc aggaatttag cctggtaaat agcaccaatc ggacccagac ccatagatac
    26101 ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag agaggccatt
    26161 gccgtgaact tcctgacgga agttatccag ctgctcctga gtcagacgac cttccaggaa
    26221 agcacgggcg taaacgcccg gggagatgtg gccctggaag taaaccaggt cgccgccatc
    26281 ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga
    26341 ctggaaggac gccatgtggc cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt
    26401 catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg
    26461 atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac ctgtgcctgc
    26521 ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc
    26581 acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc
    26641 gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata
    26701 acgttgagtt ttctggaacc tgtcccattg aactctcgcc agaaagctca ataagacagg
    26761 ttctacgttt agttaccgcg cttttatatg cgcttgattt acaacatctt ctggataatt
    26821 tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc
    26881 ggctctcttc acggcttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg
    26941 atgcttcgcg cgcttcttcc ggcttaccga ccataatcgc ttcaaatatg cgggtgcggt
    27001 gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga
    27061 cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg
    27121 ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc
    27181 cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat
    27241 cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga
    27301 gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga tcgctgaagc
    27361 tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct
    27421 tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtcg aactgttttg
    27481 ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg aggatcaaaa
    27541 actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca
    27601 tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg
    27661 acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa
    27721 ctgtgcacat tttaacaact tgacatatat aacgtttcaa agttgtaact atgcacaaat
    27781 gtagacttta cgtaggaaag ggatttttta acgattatta aactataaaa atccgaattg
    27841 aaccgattca cttaccaatt ttttgatttt taagtcaata aaaacgaatt taaattcact
    27901 ctgcatattg agaggggttg aggctgagct ttacaaacgg tttcttttta agcaacccat
    27961 cttcaaccat gcataaagcg ggtgcattcg ctgccgcata ccattattct tgatctgacg
    28021 gaagtctttt tgtaacaatt caaacctcct tgatgtaaac aaatcaatac aacaaacgga
    28081 attgcaaact tacacacgca tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt
    28141 catgatggaa ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa accgccatat
    28201 tcagcttatc gcgctgggtg gcgcgatagg gacagggcta ttcctaggta gcgcctccgt
    28261 aatacagtcc gcagggccag ggattatcct gggttacgcc attgctggtt ttatcgcctt
    28321 tctgatcatg cgtcagctgg gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag
    28381 ccactttgct tataaatact ggggcagttt tgccggtttc gcctctggct ggaactactg
    28441 ggtactgtac gttttagttg ccatggcaga gctgactgcc gtgggtaaat acattcagtt
    28501 ctggtatccg gaaatcccaa cctgggtttc tgccgccgta ttctttgtgg tgattaacgc
    28561 catcaacctg accaacgtta aagtgtttgg cgagatggag ttctggtttg ccattatcaa
    28621 agttattgcg gtagtagcga tgatcatctt cggcggctgg ctgctgttca gtggcaacgg
    28681 cggcccgcag gcgagcgtta gcaacctgtg ggatcaaggt ggtttcctgc cgcacggctt
    28741 caccggactg gtgatgatga tggcgattat catgttctcg ttcggtggtc tggaactggt
    28801 ggggatcacc gcagcagaag ctgataaccc ggagcaaagt atcccgaaag caactaacca
    28861 ggttatctac cgcatcctga ttttctatat tggttcgtta gccgttctgc tctcactgat
    28921 gccgtggacc cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc acgagttagg
    28981 cgataccttt gtggcgaatg cgctgaacat cgtggtactg actgcggcgc tctccgtgta
    29041 caacagctgc gtatattgca acagccgtat gctgtttggt ctggcacaac aggggaacgc
    29101 gccaaaagcg ctggcgtctg tcgataaacg cggcgtaccg gttaacacca ttctggtgtc
    29161 tgcgctggtt acagcattgt gcgtattgat taactatctt gctccggaat ccgcatttgg
    29221 cctgttaatg gcactggtgg tatccgcact ggtgatcaac tgggcgatga tcagtctggc
    29281 gcatatgaag ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct tccctgctct
    29341 gctttatccg ctgggtaact ggatctgcct gctgtttatg gcggcggtac tggtgattat
    29401 gctgatgacc ccaggaatgg cgatttcggt atacctgatc ccggtatggc tgatcgtgtt
    29461 aggtatcggc tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct
    29521 ctacgccctc acccgtacag ggtgagggca ataatctcta caccaacgcc ccgtaaatcg
    29581 tcagtagcgc aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg
    29641 ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac
    29701 gcgttaatac ctgatattgc gaagtatgga aatcacccag cgaagcaaac caggccggta
    29761 acgctttctc accatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc
    29821 aatccagtac atgaagcact gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt
    29881 atcgcgccag ccagtattgc catgcacgca agaacgcata ccccatcagc gtaacgggtc
    29941 cccaggttcc ccccacaatc agccagaaca gcggtgcaag ataaaaacga aagttaatcc
    30001 acagcaatgc attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc
    30061 cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag
    30121 cagctgtcag ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac
    30181 caatcagcag ccacaccagc agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta
    30241 acagtaaaaa agtcacaccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa
    30301 aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca
    30361 acttaaacag gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc
    30421 cttgctgacc agcgcacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg
    30481 atccggcgca atatcacaat ggcccgtcat gttattagcg atatcgggat agcgatctat
    30541 cagtgcccgc gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagagtatc
    30601 ggtgccttca agctcaatcc caatagaaaa atcattgcag cgttcacgcc cctgatactg
    30661 agagactccc gcatgccagg cacgtttatc gaaaggaaca tactggacta tttcaccatc
    30721 acggcgaatc aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg
    30781 tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc
    30841 tggcggcagg ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccaggcggca
    30901 atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat
    30961 gcaggatctc cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat
    31021 gattcgttag ctatctggag ttttaacatg ccgcctcgcc gctacaaccc tgacacccga
    31081 cgtgacgagc tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg
    31141 cgggaagatt taggcggaac agtcgatgcc aacaatgaca ttacggcaaa acttttaccg
    31201 gaaaattctc gctctcatgc cacggtgatc acccgcgaga atggcgtatt ttgtggcaaa
    31261 cgctgggttg aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat
    31321 gtggatgacg gcgatgtcat caacgccaat caacccttgt tcgaacttga aggcccatcc
    31381 cgcgtgctgt taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt
    31441 gccagtaagg tacgccacta tgtcgaatta ctggaaggca ccaacacgca gttgttggat
    31501 acgcgcaaaa ccttacctgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc
    31561 ggagcgaatc accgtctggg gctttctgat gccttcctga tcaaagaaaa ccatattatt
    31621 gcctccggct cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcatcc ggatgcgcca
    31681 gtagaagtcg aagtagagaa tctggaagaa cttgatgagg ccctgaaagc cggagccgat
    31741 atcatcatgc tggataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac
    31801 ggcaaggcgc tactggaagt gtctggcaac gtcactgaca aaacactgcg cgaatttgcc
    31861 gaaacgggcg tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac
    31921 ctttcaatgc gttttcgcta attgctgaca gatttatccg ctccatcgcg ggcggataat
    31981 ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga
    32041 aataaaaaac ctcggaatct gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg
    32101 gcgtttgcca aagtagcacc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg
    32161 ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa gctccattgg
    32221 tattcccgct tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt
    32281 tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa catggtggat tagatacctg
    32341 cgacggtggc agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag
    32401 tgtggcaaag ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatgggc taagcgtcgt
    32461 catgacgcca ggttgggata acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat
    32521 tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa
    32581 ctaaggagcg gcaatgaata ttccacagct cacggccctg tgcctgcgtt atcagggagt
    32641 cttgctggat gccagcgaag aagtggttca tgttgcggtg gtcgatgccc cctcacatga
    32701 gttgctggac gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg
    32761 ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca ttacccgtag ctgttcagga
    32821 gaagcatcag cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg
    32881 cgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga
    32941 cggcgtattg catcctttac cggatgtttc accggatgcc ggagtcgcat taacagccag
    33001 attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt
    33061 cactgtcgaa ctggcaggaa acaccgtctc atttcgtatt gcgaccttac catgtcgggg
    33121 tggtgaaaag gtggtattaa ggttgttaca gcaggtgagc caggcactgg atgttaacac
    33181 gctgggaatg cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg
    33241 actggtgctg gtaactggcc ctacaggcag tggcaaaacg gtcacctggt gttgtccacg
    33301 ctacacacca attccaccac tgaaacgctg gtacgtttac agcaaatggg ggtcgcccgc
    33361 tggatgctat catcggcgct tacgctggta atagcccagc gtctggtacg taaactttgc
    33421 ccacattgtc gccgacagca aggggagccc atccatattc cagacactgt atggccgtcg
    33481 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
    33541 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
    33601 actgaagttg aatcgctgga aacgcacgcc cgacaggcgg gtatgcgtac gctttttgaa
    33661 aacggctgcc tggccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
    33721 gggatgccgc atggcaagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
    33781 tgcgcaagat gggatgctat gggcagagag ccgtacttta ctgcttatgg cactacagca
    33841 acagatggtt accccactaa gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
    33901 agataaaagc gcggaagtca ttcatcaact ggcgacgcta cttaaagccg ggttaacgct
    33961 ttctgaaggg ctggctctgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
    34021 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
    34081 gtcagaggca tttccgccgc tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
    34141 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa tcccagcgtc agttgaccga
    34201 caaagtgaaa tcagcgttgc gttatcccat catcatttta gcgatggcaa tcatggtggt
    34261 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
    34321 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatgggg
    34381 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
    34441 cccgacctgg cttatcgcgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
    34501 gatgagggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
    34561 cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
    34621 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
    34681 aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
    34741 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
    34801 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
    34861 tatcggtacg ctggtggtgg caatgtatct gccaattttc catttaggcg atgcgatgag
    34921 tgggatggga taacgctggc gtgttgctcc acgccagcgt tgggagatta caggttgttg
    34981 aagatgcggt tttcttgttc cagcacacga ataaacgtgg tgcgcttggt cagctctttc
    35041 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
    35101 gtattttcaa cctggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcggtat
    35161 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
    35221 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
    35281 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg ctttcgccac atcgcccggc
    35341 gtggtacagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
    35401 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
    35461 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
    35521 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
    35581 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacgaag
    35641 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgtg
    35701 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
    35761 cactcttcga cagaaaagtg tttatgcaca gcagtcagaa tatcaaaaga agccagcgca
    35821 gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
    35881 cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
    35941 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
    36001 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
    36061 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
    36121 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcactttc
    36181 gcgcgctcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatggg
    36241 gtatatagtt gccttaacgg gaggcattgg cagtggtaag agtaccgttg ccaatgcgtt
    36301 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
    36361 aggtgcacct gcgctacatg ccattgctga tcactttggc gaaaacatga ttgctgctga
    36421 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
    36481 ctggcttaac gacctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
    36541 agcaacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
    36601 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
    36661 gcagcgcgat gatgtaactc gcgaacatgt cgaacaaatc cttgctgctc aggcaacgcg
    36721 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
    36781 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
    36841 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
    36901 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
    36961 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
    37021 cgcggcgaag tccgcactga gctgttgaaa gaacttaacc ggcagcaacg taaactccag
    37081 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
    37141 aaagcggcgg tgagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
    37201 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
    37261 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
    37321 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
    37381 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
    37441 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
    37501 tccggacata agagccgttt tgccattcgt tttatgccgc tggacactga aaacggacag
    37561 gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtaaaaat gtcagaaact
    37621 attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt
    37681 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
    37741 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
    37801 cagtgacatt tgtctgatgc ctcacgtagg cctgataaga cgcggacagc gtcgcatcag
    37861 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gacgcttaag
    37921 cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac cgaccagcga
    37981 catccactca ccgggttgcc cttctttacc ccacggcacc ccttcccaac tttcgaccag
    38041 ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga
    38101 aaaatgttgt ggggtaatcc cgacctcttc ctgaagttca cgcaccaccg cctgttccgg
    38161 cgtttcgccc atttcaattt taccgccggg aaactccagt ttattcgcca tgtgcgcatc
    38221 tgctgcgcga cgtgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat
    38281 ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact
    38341 tcaactgtta gcttttattg caggcgacca tggcactgct tgtatttttt accagaaccg
    38401 cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct
    38461 gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgtgctaa acgctcggct
    38521 tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg
    38581 ctcagcgtac tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac
    38641 gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc
    38701 tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag cgtttgcagc
    38761 atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga
    38821 tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttctggt
    38881 tctttatcca gccactcggt aattggtaaa tcgaggtcga aatcgttctt cagacgttcc
    38941 tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg
    39001 gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc
    39061 agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat
    39121 tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg ggcgttggca
    39181 atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc cagtttacgc
    39241 atcatgccgg atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg
    39301 tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa ctggttatcg
    39361 atacgacggg attcgtgacg ctcggtaccg atgatatgca ggccacctgc ttccagtacc
    39421 gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc ggtcggattt
    39481 tccagcgcgg caacttctgc ctgccagcta cctccgagca caatatctgt accacgaccc
    39541 gccatgttgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac aatcgccgct
    39601 tcgttggcgt ggaatttggc gttcaggaca ttgtgcttaa taccggcttt ggtcagttcg
    39661 tttgacacca gctccgattt ttcgatggag atagtaccca ccagcaccgg ctggcctttc
    39721 gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag
    39781 accaggtccg gcagatcttt acgaatcatt ggacggttgg tcggaacaac gacggtatcc
    39841 agtttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt catccccgcc
    39901 agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg
    39961 ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc ggaccagcgt
    40021 cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg
    40081 atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc
    40141 atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat gccctctttc
    40201 accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg gcgagatttt
    40261 tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg
    40321 atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgccggg
    40381 ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac ttcgtccacc
    40441 agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat gttgtcgcgc
    40501 aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc gtaagcttcg
    40561 cgctttgccg gtgctggcat gcccggcagg ttgataccaa cagtcaggcc aaggaattca
    40621 aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac ggtaactacg
    40681 tgcacgcctt taccggttag tgcgttcagg taagcaggca gcgttgcggt cagggtttta
    40741 ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac
    40801 tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgcac cacggcgaaa
    40861 gcttccggga tcagattttc cagcacttcg cctttttcca gacgcgcacg aaactctgcg
    40921 gttttccctt tcagttcttc atcggagagt ttttccatct ccggttccat ggcattgatg
    40981 atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta
    41041 gttaacaatt tgattagcat aataaaatct caaacgcccc gcgttacgga gttaataaaa
    41101 tgaagtaaag gtttattgtt gttaggtgag gcgttgaggg ccagcacgga tgccttgcgc
    41161 ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat
    41221 gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga gcgtatccag
    41281 taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca gtgtttgcgg
    41341 tgccattgcg aaagaaagat gacggattac cgtacgaata gcatgttgat gccagtaatc
    41401 aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa
    41461 gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc
    41521 ggcggcgttg ctgagcgcag gcaaacctaa actcgccgcg accatcccta ataagagatg
    41581 cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt
    41641 gccatcccga tcgaattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt
    41701 ccacagcacg aatgaagata agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc
    41761 gttttttata gctcttataa aattcaaaaa aaaacgactg gttcacctgg ccggagagag
    41821 tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct
    41881 gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag
    41941 gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta
    42001 taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg
    42061 agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga
    42121 taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc
    42181 agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac
    42241 gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta
    42301 aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg
    42361 ccatcttcga cacgaacagt ctctttgatg cgaacaaatt ttttggcgca gttcaactcg
    42421 tcgataccgg cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg
    42481 atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca
    42541 ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc
    42601 atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg
    42661 cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt gactttcttg
    42721 ccggtatgta aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc
    42781 atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt cggcgggcca
    42841 gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag cttgcttacg
    42901 taggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt
    42961 cactacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccat gctgctggta
    43021 gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt
    43081 gtccatgccg atacctgtcg caacaacggt tacgcgcagc tcgtcattca tatccgggtc
    43141 aagagaagta ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc
    43201 taccgtttcg aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagcacgcc
    43261 gcgcgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc
    43321 ttcttccgca cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatttc
    43381 agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag
    43441 ttcggcgata ccttgcacag cgcctttcag tacatcgttc gctgcgccaa acgcatccag
    43501 cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga
    43561 gtccacatgc ttggacagtt cagtgatccc ctgctcggcg aatgccatac gcttcttgcc
    43621 ttcaaagttg aaaggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac
    43681 ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa
    43741 gaccatgtct gcaccttcca gcgccgcacg caatgcatcg cgatcctcat cagccgcatt
    43801 gcggccaact tctggattag cgccagcgcc cagtcctttg gtgataccgc taccgatttg
    43861 aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa
    43921 ttcaacacct tcaatacgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc
    43981 gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc
    44041 tctccgattt gtgcctgtcg cctgagtccg taatcatcgt cggcctcata aaaattaaaa
    44101 ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg
    44161 tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac
    44221 cgccgtcgaa taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc
    44281 gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg
    44341 cgctgcgcca ccggttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg
    44401 aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcaact cggtatagcg
    44461 cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac gaccgcctac
    44521 gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg
    44581 aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt
    44641 cacgacattg ccagcataag gaattacctt tgtgtggcgc aatgccccac cggtataaac
    44701 ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt cacgttcatc
    44761 ttccgtcaat accgaataac ttgatgccag tccggcaaat atcagttggt caactttcag
    44821 cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac atgtgatcag
    44881 gtgcactttt gcctgcatcc gcacacccga aagtcctacc ggattcttga tcccttcctg
    44941 atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat cgcgcacacg
    45001 caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga
    45061 aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata
    45121 taccgaagag atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca
    45181 cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgtg acgggcagct
    45241 gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac
    45301 cttcgcggta ccaatttcca gtcctactac cagttttctg tccgtcgcct tgatcattgt
    45361 tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag
    45421 cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt
    45481 tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gaaccaaacg tttcatcgta
    45541 tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc
    45601 gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg
    45661 cgatagccct gcaacacttc attagcgctg ccttccgggc catacagcat tggaagcacc
    45721 tgcttgctgg tgcgatctgg cggcacgctg aaggtgtttc cttccgcgtc taccatgtgt
    45781 tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc
    45841 cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg
    45901 atgatgttga catcctgggt cataaaggta cccggctcac ccaatgccag gatcgactgc
    45961 cggatatcgt cattacgcgt gtaatggcgt tcaccggtca acaccagctt tgagagcggc
    46021 aggcgttgcg catcttccat ccagctcaac acgacccagc cgctcaccaa cactgtcgtt
    46081 aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa
    46141 acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc
    46201 cagaattcgt actaccaact gcgagaagct catacctgcc tgacgtgccg ccatcggcac
    46261 caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc
    46321 actgtccagc ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt
    46381 cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg ggcagaaata
    46441 ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg acggttgaat
    46501 acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag
    46561 ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag
    46621 agcattttct gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac
    46681 gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcgctca ggcctttttc
    46741 aaactctgcg cgggttaacg ctacccacgg cgcgaccggt aaaccggcac cttgccacag
    46801 aagtttgctg cgtagtttat ctattgaaag cgcagatgcc atcactccgc tgccggtata
    46861 aggcaagccc atcagctcga gcatgccctg cagcgtacca tcttcaccgc cgcgaccgtg
    46921 tagcgcgata aacactttct gaaaacccat cgacttcagt tgcgtcacgt cgacttcttt
    46981 cgggtcgaca ggatacgcat caataccgcc ttcacgcagc ccggctaaca ccgctgcgcc
    47041 agaattcaga gaaacttccc gctcagcgga agtcccgccc aacaggaccg cgattttatc
    47101 agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg
    47161 caatttttcc gatattacca gccccctgaa cgagaatcag gtcgttaccg gttaataccg
    47221 gtgccagcat ctcggctacc tgcgcagggt ccggcaccag aatgggatca attttcccac
    47281 gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag
    47341 ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat
    47401 catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat
    47461 ccggccagcc tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg tggtggccgt
    47521 agtcatcgac cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac
    47581 cgaggaaatc aaaacggcga ccagtcccct ggaagctctc cagcgcacgc aaaatagcct
    47641 cgtcgtcaat tccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat
    47701 gacggcctgg cgcattcagg gtgacgcaca tcggctcttt gtcctgacgc agcagcgtaa
    47761 agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga
    47821 agccgtaagt tgtggtctga cgtcccacac gcggtaacaa ttcgcggatc accggatcat
    47881 caacacacat caccgcacga ccgtaaaaag gcaggttgtg cagaaaatta ataaaggtct
    47941 gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga
    48001 caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa
    48061 tcaggtaccg accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt
    48121 taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggaaaccatc gcggtagttg
    48181 tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt aactcagcca
    48241 gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt
    48301 tgtcggcaga aatggcgctg gaaacaacga ccacgctggc atcacgtacg ttttccgggc
    48361 gatggttgaa ataaatcgtc gcacccagat tcagtaactg ctgcgtgacc ggatttggcg
    48421 ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca ataccgccca
    48481 taccggcacc accaatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga
    48541 tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt
    48601 catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc
    48661 cactcgctcg gtggcatccg gaatggatgc agcgcgggcg cgttctgcca tggttaataa
    48721 ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg
    48781 ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg
    48841 gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac
    48901 agttaaagca ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc
    48961 atcaataaat tcagtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac
    49021 ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac ccagtttcgc
    49081 agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccaa
    49141 cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggca gcgccagcac
    49201 atcggtacgc accgggttac ccaccacttc cgcattaggg aaagcgcctg gaaacgcctg
    49261 catcactttg gtagcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg
    49321 ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccacctg gacctgatac
    49381 atagccgccc ataccgagta ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg
    49441 acgccaggcg ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg
    49501 caaaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc
    49561 catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatagtgcgc
    49621 aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt
    49681 tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca
    49741 taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg
    49801 taactgatta gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg
    49861 ttaaccagcg cctggaagct aaaccagatg ccaatagaac aggcgagaaa accggaaaaa
    49921 cggtggtcaa tttctaatgc tttgcggcca atcgacatcg cacgaaaagc gacgaagaat
    49981 accattaaaa gtgctagtac cacaccgaca taacctaatt cttcgccgat aatggcgaaa
    50041 ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgaatt acctaaacct
    50101 tgcccccaaa gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg
    50161 ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg
    50221 gcgaggatca gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac
    50281 aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac caccaccgta
    50341 ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc
    50401 aggaagccgc gcaggttatt acgtacttcg tcgcctttac gcaccagata gttggcgata
    50461 tagcaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg
    50521 atccaacgcg atgccccttt aacagcgcta cccactacca gcacgatcat cagcaggatg
    50581 atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc
    50641 gtaatgatcg ccagaataaa cgccaaaatc agatagacac catcacgctt cgcgaagaag
    50701 aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag
    50761 ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg
    50821 tcggtatctt tttcccgcga gcccatcacc cagcccttta gcgccgtgga gatccagacc
    50881 aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac
    50941 ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat
    51001 caaggctggc acaggccggg gagagcagaa ccatatcgcc cggctgaaca cgcggagcca
    51061 gcaagcgcat agcctgttcc atagtttcgg tttgttctgc cacttccggg cgtagcgccg
    51121 ccagctgcgc gccgtcacga ccgaaacaat acagacgtac gttatcgcca ttcaggtaac
    51181 gcaccagtgg gctaaagtcc gccgatttac catcgccgcc cagcaacaaa tgcagcgtgc
    51241 cgtctacgtg caggccattc agcgccgctt ccgtactgcc gacgttggtc gcttttgaat
    51301 cgttaatcca gcgcacaccg ttatgctcca gcaccacttc aaagcgatgc ggcagaccgg
    51361 tgaaggtggt taacgctttc aggctgctgg cgcgcggtaa ccctgcggca tctgccagcg
    51421 ccagcgccac cagcgcattg gtgtagttat gctgcccgga aagtttcatc tctttcacat
    51481 tcagcacttt ctcgccttta acccgcagcc aggtttcgcc ctgctgatga ttcaggtgat
    51541 agtcacccat gttgacgcca aagctgacgc agcgttcatc cgcaccgcga atcggcattg
    51601 ttaaggcatc atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg cgcagttttg
    51661 ctgcacgata ctgttgtaaa ccaaacgggt agcgatccat atgatcttca gtcacgttca
    51721 gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc cagctggaag ctcgacagtt
    51781 ccagcacgta cagttcacac tcatcatcca gtagcatcaa cgcaggcagg ccaatattgc
    51841 cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg
    51901 tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cgacaaaaca
    51961 gctcgatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg
    52021 ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca
    52081 gactgcccgt gtggcgttct acggcttcgg gtaatttatc caggccaggc ggtgtcatac
    52141 gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc
    52201 cggtgaggcc caggccgata atgacaacat ttttaccctg gtaatcagcc atgattaacg
    52261 taccttcagc gttgccagac caatcaggac cagcatcagc gaaataatcc agaaacgcac
    52321 aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg
    52381 gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc
    52441 taccacgaac acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat
    52501 aatgcctaac gcaccaccta acgccagcga accgacatcg cccataaaga cctgcgccgg
    52561 ataggtgtta aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac
    52621 cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc
    52681 ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag
    52741 accgtcaaga ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc
    52801 cagcagaatg tagaacagcc ccagttgcgg catcacatct ttaaagaatg ggaccaccag
    52861 ctgcgttgcg ggcgtgtctt tgccggcaag gtacaaagcg aaagcaacac ccagcgcaat
    52921 gaccgacatc cagaaatatt tccaacgagc gatcaaccct ttggtgtctt tacgcaccac
    52981 tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca
    53041 ccagacgtac ggattggacg ggtaagccca cagcagcacg gagatcacaa tcgctgtcag
    53101 gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc
    53161 gttacgcact acctgaccaa aggaaagttt ttgcaaatga gcaatcatgc gcgggcccat
    53221 ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga
    53281 aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc
    53341 ccattctcct gtaaagcgcg tactacctct tccatggcag cactacgtga acccttaact
    53401 aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt
    53461 ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc
    53521 atgcttaaca cgcggtcaat accagcagct tttgccgcct cacccacctg tacatggcag
    53581 gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc
    53641 atttcagcca gtacctgaac agctgcggtc attgaaccga cattggcgtt gtaggagtcg
    53701 tcgagcagca actggttttc tgccagttgg atggggaaca gacggccagg aacagctttc
    53761 agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca
    53821 gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag aacatcgacg
    53881 ctaccggttg gggtttgtag ggtaaattcc ataccgtgcg aggtcacatg gatattggtg
    53941 gcggtgaaat cgctgttggc ggcattgggt gagaaacgcc acactttgcg tgagccaatt
    54001 acgctctgcc agttcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc
    54061 ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct
    54121 tccagatgcg ccgctgccag gttgttgacc agcgccgctt ccgggcgagt cagactcaca
    54181 gtccaggcaa tctcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc
    54241 ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta
    54301 taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg
    54361 ccggaggacc ccgtcagagc aaccacgagc gccggaactt gctggcgaac ccatgcagcc
    54421 agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc gatgtccagc
    54481 ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc aaaatcgtgg
    54541 gcatcaaaac gttcaccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta
    54601 tcagtggtta cagcatcaag ggtgatatct gcaccttgca gttcaccgtt gagaatatcg
    54661 gtaagttggc taagggttac gctaatcatg caatcccccc cagcagacgc gccaccgtga
    54721 cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcctca tggcctttgc
    54781 ccgcgaccag taccacatca ttctctttag cctgcataac ggcgcaagtc accgcttcag
    54841 cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga
    54901 tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc acgtcagcaa
    54961 actcttcggc aattgcgccc atcagtggac gcttaccttt atcgcgatcg ccaccacagc
    55021 caaagacgca ccacagcttg cccgcacagt gcaggcgcgc cgcctgtaag gctttttcca
    55081 gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc gcagtgaaca
    55141 cttccatacg tccgcaaacc ggttgcagac gcgcggcggt tttcagcaga tcagccagtg
    55201 gatagcccag cgccagcagt gttgccagcg cgagcaacag gttgctgacg ttaaaagcgc
    55261 ccatcagatg gctttcaatt tcgccatcgc cccagcttga actaaagcga atggtcgcgc
    55321 cgctgtcgtg atagttcact tcggtcgctt tcaaccagcg tccgtgacag ttcggattga
    55381 tatgatcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag cggcggccca
    55441 cttcatcgtc ggcgttaata atcgcctgac cgcaatgatg ttcagaataa agcagccatt
    55501 tcgcagcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg
    55561 taaagaccga cgccggaaat ttcaatgccg ccacacggtg ctgtaccagc ccgtgggagg
    55621 aaacttccat tgcgcaaaaa gtcgcgccct gatccaccag ccccgccagc tcatgctgaa
    55681 catcaactgc cgaaccggta gtattttctg tcgggatcac tttccccagc agaccgttac
    55741 caacggtgcc cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagaagct
    55801 gggtcgtcgt ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa ttgtcagagg
    55861 gttcatggta aaagcggccc gccagtgcag ataaacgctc gttgagctgg ctgagataga
    55921 tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcatct ttcgcctctg
    55981 caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct
    56041 gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca
    56101 tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat
    56161 tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt
    56221 gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag
    56281 accggcgcgg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca
    56341 accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta
    56401 ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat ggcgatacga
    56461 tagcctttaa tcgccgcctt cacgccgccg ccgcctggta gcgccacgct ttccatcata
    56521 tgcaccacgg tgcgaacaat ggattccggg aagacacgtt caccgggaac cggggggtca
    56581 actttggtaa tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc
    56641 gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg
    56701 tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg
    56761 gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acgctgagga cggcatcgtt
    56821 aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat
    56881 tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg
    56941 agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg tcatcaccac catcggttta
    57001 accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctctttcggc
    57061 gtgccgctca gattgttagg gttgtatgac gggctgttag ccatcgccag cacttcacct
    57121 gtgttgacat ccaccagcac ggcgctacca gattcagcct tgttaaaggc caccgcgttg
    57181 ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc
    57241 gctgcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc
    57301 acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc aacgccctca
    57361 atcccttgac tatcgacgtt agtaaagccg atgaggtgag cagtcacttc gccggacgga
    57421 tagtaacggc gagactcttc acgcagatga atccccggca gtttcagttt tttgatgtag
    57481 tccgccatgt cagagtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg
    57541 ttaatgcggg cagaaagctg atccagcgga atattgagcg cgttagccag cgccttccag
    57601 cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct
    57661 tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag
    57721 gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg
    57781 gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag
    57841 ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg acgttttggt
    57901 ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata
    57961 ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc
    58021 cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa
    58081 gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc
    58141 gtggttacca cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc
    58201 ccaaatcgca aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc
    58261 atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct
    58321 ctgcaatacg cagaactgaa ctacgggcac gagggttctc agccacctct tcttcgcccg
    58381 gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc tgctcttcag
    58441 tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca
    58501 caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgcccaccc ggggccagca
    58561 cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc
    58621 gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag
    58681 cagccacgac ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga
    58741 caatggcgcg ggcaatgcgt ttggcaaaat gctcttcacc ataggttttc aatacccagg
    58801 cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt
    58861 ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgttca gcatcatcaa
    58921 gttgcggtga agagacgcca agatcgagga gaatgccgtc gatcttgcca ataagatcgc
    58981 gctcggcaac gtattcgccc agcgcggaga aaggtccgtg gatgatggag aagcgcggat
    59041 catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc
    59101 cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat
    59161 caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc agcagcaccg
    59221 tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag
    59281 tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt
    59341 tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg
    59401 gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg
    59461 ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat gctcaacaga
    59521 cgcgataatt tttgctcgat aatttcccat tcaggcaggg ggtaaagcag caggcacggg
    59581 tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga
    59641 taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg
    59701 aacatgccag cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt
    59761 tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg
    59821 atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgaag caaattaacg
    59881 ttatgaatag ctgtaagaaa aaatccctca tcgtcatcac tgttttaaaa tgactcacta
    59941 ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct
    60001 tccccggtta acagtccttg ttacgtctgt gtggcgcttg atgccagcgg gtttgcgcaa
    60061 aagcaaagaa aagtgtttgt taaatcggca attcatcgcc gcatgtaaca aaataatgca
    60121 aaaacgcgct gtcgatatca cttaattatt gtgaatttca gctggtgagt taattaacgg
    60181 gcgggtttaa aaccatttat caataatagt caggaatagt cttatttact ttaa
//
LOCUS       DAJVPT010000010        57787 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVPT010000010 DAJVPT010000000
VERSION     DAJVPT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            190..762
                     /gene="yeiP"
                     /locus_tag="OQM47_000621"
     CDS             190..762
                     /gene="yeiP"
                     /locus_tag="OQM47_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1478899.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            985..2451
                     /locus_tag="OQM47_000622"
     CDS             985..2451
                     /locus_tag="OQM47_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1478900.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2569..3555
                     /gene="yeiR"
                     /locus_tag="OQM47_000623"
     CDS             2569..3555
                     /gene="yeiR"
                     /locus_tag="OQM47_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1478901.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3594..4307
                     /gene="lpxT"
                     /locus_tag="OQM47_000624"
     CDS             3594..4307
                     /gene="lpxT"
                     /locus_tag="OQM47_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1478902.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4719..5285
                     /gene="mepS"
                     /locus_tag="OQM47_000625"
     CDS             4719..5285
                     /gene="mepS"
                     /locus_tag="OQM47_000625"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1478903.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5466..7022
                     /locus_tag="OQM47_000626"
     CDS             5466..7022
                     /locus_tag="OQM47_000626"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1478904.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7104..8918
                     /locus_tag="OQM47_000627"
     CDS             7104..8918
                     /locus_tag="OQM47_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1478905.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8919..10013
                     /locus_tag="OQM47_000628"
     CDS             8919..10013
                     /locus_tag="OQM47_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1478906.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10013..11038
                     /locus_tag="OQM47_000629"
     CDS             10013..11038
                     /locus_tag="OQM47_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1478907.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11040..12629
                     /gene="yejF"
                     /locus_tag="OQM47_000630"
     CDS             11040..12629
                     /gene="yejF"
                     /locus_tag="OQM47_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1478908.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12633..12977)
                     /locus_tag="OQM47_000631"
     CDS             complement(12633..12977)
                     /locus_tag="OQM47_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478909.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13310..14500)
                     /gene="bcr"
                     /locus_tag="OQM47_000632"
     CDS             complement(13310..14500)
                     /gene="bcr"
                     /locus_tag="OQM47_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1478910.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14528..15223)
                     /gene="rsuA"
                     /locus_tag="OQM47_000633"
     CDS             complement(14528..15223)
                     /gene="rsuA"
                     /locus_tag="OQM47_000633"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1478911.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15373..17133
                     /locus_tag="OQM47_000634"
     CDS             15373..17133
                     /locus_tag="OQM47_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1478912.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17258..17542
                     /gene="rplY"
                     /locus_tag="OQM47_000635"
     CDS             17258..17542
                     /gene="rplY"
                     /locus_tag="OQM47_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1478913.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17681..18688)
                     /gene="yejK"
                     /locus_tag="OQM47_000636"
     CDS             complement(17681..18688)
                     /gene="yejK"
                     /locus_tag="OQM47_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1478914.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18870..19097
                     /locus_tag="OQM47_000637"
     CDS             18870..19097
                     /locus_tag="OQM47_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1478915.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19117..20877
                     /gene="pbgA"
                     /locus_tag="OQM47_000638"
     CDS             19117..20877
                     /gene="pbgA"
                     /locus_tag="OQM47_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1478916.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20952..21028
                     /locus_tag="OQM47_000639"
     tRNA            20952..21028
                     /locus_tag="OQM47_000639"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20986..20988,aa:Pro,seq:ggg)
     gene            complement(21131..23722)
                     /gene="yejO"
                     /locus_tag="OQM47_000640"
     CDS             complement(21131..23722)
                     /gene="yejO"
                     /locus_tag="OQM47_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1478917.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24042..24689
                     /gene="narP"
                     /locus_tag="OQM47_000641"
     CDS             24042..24689
                     /gene="narP"
                     /locus_tag="OQM47_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1478918.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24900..25952)
                     /gene="ccmH"
                     /locus_tag="OQM47_000642"
     CDS             complement(24900..25952)
                     /gene="ccmH"
                     /locus_tag="OQM47_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1478919.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWLQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25949..26506)
                     /gene="dsbE"
                     /locus_tag="OQM47_000643"
     CDS             complement(25949..26506)
                     /gene="dsbE"
                     /locus_tag="OQM47_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1478920.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26503..28446)
                     /gene="ccmF"
                     /locus_tag="OQM47_000644"
     CDS             complement(26503..28446)
                     /gene="ccmF"
                     /locus_tag="OQM47_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1478921.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28443..28922)
                     /gene="ccmE"
                     /locus_tag="OQM47_000645"
     CDS             complement(28443..28922)
                     /gene="ccmE"
                     /locus_tag="OQM47_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1478922.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28919..29128)
                     /gene="ccmD"
                     /locus_tag="OQM47_000646"
     CDS             complement(28919..29128)
                     /gene="ccmD"
                     /locus_tag="OQM47_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1478923.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29125..29862)
                     /gene="ccmC"
                     /locus_tag="OQM47_000647"
     CDS             complement(29125..29862)
                     /gene="ccmC"
                     /locus_tag="OQM47_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1478924.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29904..30566)
                     /gene="ccmB"
                     /locus_tag="OQM47_000648"
     CDS             complement(29904..30566)
                     /gene="ccmB"
                     /locus_tag="OQM47_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1478925.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30563..31180)
                     /gene="ccmA"
                     /locus_tag="OQM47_000649"
     CDS             complement(30563..31180)
                     /gene="ccmA"
                     /locus_tag="OQM47_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1478926.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31199..31801)
                     /gene="napC"
                     /locus_tag="OQM47_000650"
     CDS             complement(31199..31801)
                     /gene="napC"
                     /locus_tag="OQM47_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1478927.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31811..32260)
                     /gene="napB"
                     /locus_tag="OQM47_000651"
     CDS             complement(31811..32260)
                     /gene="napB"
                     /locus_tag="OQM47_000651"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1478928.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32257..33120)
                     /gene="napH"
                     /locus_tag="OQM47_000652"
     CDS             complement(32257..33120)
                     /gene="napH"
                     /locus_tag="OQM47_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1478929.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33107..33802)
                     /gene="napG"
                     /locus_tag="OQM47_000653"
     CDS             complement(33107..33802)
                     /gene="napG"
                     /locus_tag="OQM47_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1478930.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33809..36295)
                     /gene="napA"
                     /locus_tag="OQM47_000654"
     CDS             complement(33809..36295)
                     /gene="napA"
                     /locus_tag="OQM47_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1478931.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36292..36555)
                     /gene="napD"
                     /locus_tag="OQM47_000655"
     CDS             complement(36292..36555)
                     /gene="napD"
                     /locus_tag="OQM47_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1478932.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36545..37039)
                     /gene="napF"
                     /locus_tag="OQM47_000656"
     CDS             complement(36545..37039)
                     /gene="napF"
                     /locus_tag="OQM47_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1478933.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37148..37312
                     /locus_tag="OQM47_000657"
     CDS             37148..37312
                     /locus_tag="OQM47_000657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478934.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37447..37935
                     /gene="eco"
                     /locus_tag="OQM47_000658"
     CDS             37447..37935
                     /gene="eco"
                     /locus_tag="OQM47_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1478935.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38057..39088)
                     /locus_tag="OQM47_000659"
     CDS             complement(38057..39088)
                     /locus_tag="OQM47_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1478936.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39322..39681)
                     /locus_tag="OQM47_000660"
                     /pseudo
     CDS             complement(39322..39681)
                     /locus_tag="OQM47_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39899..41542)
                     /gene="yojI"
                     /locus_tag="OQM47_000661"
     CDS             complement(39899..41542)
                     /gene="yojI"
                     /locus_tag="OQM47_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1478937.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41618..42268)
                     /gene="alkB"
                     /locus_tag="OQM47_000662"
     CDS             complement(41618..42268)
                     /gene="alkB"
                     /locus_tag="OQM47_000662"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1478938.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42268..43332)
                     /gene="ada"
                     /locus_tag="OQM47_000663"
     CDS             complement(42268..43332)
                     /gene="ada"
                     /locus_tag="OQM47_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1478939.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43406..44461)
                     /gene="apbE"
                     /locus_tag="OQM47_000664"
     CDS             complement(43406..44461)
                     /gene="apbE"
                     /locus_tag="OQM47_000664"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1478940.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44573..45694)
                     /gene="ompC"
                     /locus_tag="OQM47_000665"
     CDS             complement(44573..45694)
                     /gene="ompC"
                     /locus_tag="OQM47_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1478941.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46432..49104
                     /gene="rcsD"
                     /locus_tag="OQM47_000666"
     CDS             46432..49104
                     /gene="rcsD"
                     /locus_tag="OQM47_000666"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1478942.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49121..49771
                     /gene="rcsB"
                     /locus_tag="OQM47_000667"
     CDS             49121..49771
                     /gene="rcsB"
                     /locus_tag="OQM47_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1478943.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49971..52820)
                     /gene="rcsC"
                     /locus_tag="OQM47_000668"
     CDS             complement(49971..52820)
                     /gene="rcsC"
                     /locus_tag="OQM47_000668"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1478944.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53095..53871)
                     /locus_tag="OQM47_000669"
     CDS             complement(53095..53871)
                     /locus_tag="OQM47_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1478945.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53876..55525)
                     /locus_tag="OQM47_000670"
     CDS             complement(53876..55525)
                     /locus_tag="OQM47_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1478946.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55526..56085)
                     /locus_tag="OQM47_000671"
                     /pseudo
     CDS             complement(55526..56085)
                     /locus_tag="OQM47_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56244..>57787)
                     /locus_tag="OQM47_000672"
     CDS             complement(56244..>57787)
                     /locus_tag="OQM47_000672"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1478947.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14140 a        15602 c        14185 g        13860 t
ORIGIN      
        1 gtgcaacacg ccccgcaacc cggacggcat tccatacgtt gttctcttaa ggttaaccac
       61 cggattgatg agcgcgcaca gtaccacctt ttttgcacca gcaaaagtgc gaataccact
      121 tgcccgaaag gcccgtcgcg agtactttgt cgcgatattt ttgacatttt cgactacagg
      181 aatttttcga tgccaagagc gaacgaaatt aaaaaaggta tggtactgaa ttacaacggc
      241 aaactgctgc tggctaagga tattgatatt cagtcgccca ctgcccgcgg tgccgctacg
      301 ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga aagtagaaga gcgtttcaaa
      361 ggtgatgata tcgttgacac cgtgacgctg acccgccgtt acgttgactt ctcctatgtc
      421 gatggcaacg aatatgtctt tatggataaa gaagactata ccccgtatac cttcaccaaa
      481 gatcagattg aagaagagtt gctgtttatg ccagagggcg gcatgccgga catgcaggtg
      541 ctgacctggg acggtcaact gctggcgctt gagctgccgc agaccgttga tctggaaatc
      601 gttgaaaccg caccaggcat caaaggggca tctgccagcg cccgtaacaa accggcgaca
      661 ttgagcactg gtctggtgat tcaggtacca gaatacttaa gcccgggcga aaaaattcgt
      721 atccatatcg aagaacgccg ttatatgggg cgtgctgact aacttcagcc gcatgcagaa
      781 aagggatagc tcaggctgtc ccttttttaa tttattatac caatcttcta ttttgcgctt
      841 cctgaacacc ttacgccctc aattttcact cgttgattga tcgccctcac acttcatcgc
      901 attaacaatc cagaccaatt tcaattgctg tcatataact ttacactgtc attgttaatt
      961 tatcgttact aagacgtgac tcctatgaag acaattgcct ccgttacgct cccgcatcat
     1021 gtacacgctc cacgctacga tcgccagcag ttgcaatcac gtatcgttca ttttggcttt
     1081 ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc gtgtgctgaa tgcccagggc
     1141 ggcgactggg ggatctgtga aatcagcttg ttcagcggtg atcaactgat gagccagctc
     1201 cgcgcacaga accatttata taccgtgctg gagaaaggtg cggacggcaa tcaggtgata
     1261 attgtcggtg ccgttcacga atgccttaat gcaaaactgg attccttagc ggcaattatt
     1321 gagaaatttt gcgagccaca ggtggcaatt gtttccctga cgattaccga aaaaggctat
     1381 tgtattgacc cggccaccgg tgcactcgac accagtaatc cgcggattat tcacgatcta
     1441 caaacccctg aagaacctca ctccgcaccg ggtattctcg tcgaagcact gaaacgccgc
     1501 cgtgagcgca gccttacacc gtttaccgtg ctctcctgcg acaatattcc cgacaatggc
     1561 catgtggtga aaaacgcggt gctgagaatg gcagaaaaac gttcgccaga actcgccggg
     1621 tggataaaag aacacgtcag ttttccggga accatggtcg accgcattgt tccggctgca
     1681 accgacgaat cactggcaga aatcagccag catctggggg taaatgatcc ctgcgcgatt
     1741 agctgcgaac cgtttatcca gtgggtggtt gaagataact tcatcgctgg gcgtcctgcc
     1801 tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc catgggaaga gatgaaactg
     1861 cgtatgctta atggcagcca ctcttttctc gcttatctgg gttacctctc aggattcgcc
     1921 tatatcagtg attgcatgca ggatcgcgta tttcgctatg ctgccagaac attaatgctg
     1981 gatgagcaag cgccgacact gcaaattaaa gatgtcgatt taacacaata tgcggataag
     2041 ttaattgcac gttttgctaa tccgtcgctg aaacataaga cctggcaaat cgcgatggat
     2101 ggcagccaga aattaccgca acgtatgctg gcaggtattc gcatacatct ggggcgtgaa
     2161 acggactggt cgttgctggc attaggcgtt gctggctgga tgcgttacgt cagcggcgtt
     2221 gatgatgccg gaaatgccat tgatgttcgc gatccgctta gcgataaaat tcgcgaactt
     2281 gttgcgggca gcagcagtga acaacgcgta accgccctgc tttccctgcg tgaagttttc
     2341 ggtgatgatc tgccagataa cccgcatttt gtgcaggcca tcgaacaagc ctggcaacaa
     2401 atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc tcaaaattta acgatttctg
     2461 cggttaaagc ggatgaagct caccttcgtc cgctctcccc ttctcttttc tgcctttttt
     2521 agccaggatt aacgctcagt taacttacca gagtattgcg gagccgttgt gaccaggacc
     2581 aacctcatca ccggttttct cggcagcggg aaaaccacgt cgattcttca tctgttagcc
     2641 cataaagatc ccaacgaaaa atgggcggta ctggttaatg aatttgggga agtcggaatt
     2701 gatggtgctt tgctcgccga tagcggcgca ttgctgaaag agatccccgg cggctgcatg
     2761 tgctgcgtta atggtttacc catgcaggta gggttgaata ccttactgcg tcagggaaaa
     2821 ccagaccgct tgttgataga gccgaccggg ctgggccatc cgaaacagat cctcgatctg
     2881 ttaaccgcac cagtctatga accgtggata gatctgcgcg ccaccttgtg cattctcgat
     2941 ccacgcctgc tgctggacga aaaaagcgcc agcaatgaaa acttccgtga ccagctggct
     3001 gccgcagaca tcattgtcgc caataaatcc gaccgtgcga cgcccgaaag tgagcaagcg
     3061 ctacagcgtt ggtggcagca aaatggtggc gatcgacaat taattcacag tgagcatggg
     3121 aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt tagccgagtt gcccgccagc
     3181 gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag cagcgttaag cctgccagag
     3241 catcaacgct ggcgtcgcag tctgaacagc gggcaaggat atcaggcctg cggctggata
     3301 ttcgacgctg atacggtatt cgacaccatt ggcattctgg aatgggcgcg acttgcaccg
     3361 gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc tggtacgaat caaccgtcag
     3421 ggcgatgacc tgcacattga aacgcaaaac gttgcgccac cggacagccg tattgagctg
     3481 atttccagca gcgaagctga ctggaatgcc ttgcagagcg cgctgttgaa gcttcgttta
     3541 gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat aattagagaa aatatgatta
     3601 aaaatttgcc gcaaatagtg ttgttgaata ttgtcggccc cgcgctgttt ctttcctggt
     3661 atattcccgt taatcatgga ttctggttgc cgattgatgc ggatattttt tatttcttta
     3721 atcagaaact ggtcgaaagt aaggcctttt tatggctggt tgcattgact aacaatcgcg
     3781 ccttcgacgg ttgttcactg ctggcgatgg gtatgttgat gctgagtttc tggctgaaag
     3841 aaaacgcccc tggcagacga cgtatcgtga ttatgggtct ggtcatgcta ttaactgcag
     3901 tggtattaaa ccagctgggt caggcattaa ttcctgtaaa acgggccagc ccaacattga
     3961 cttttaccga tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa gatgcctcac
     4021 gagatagctt tcccggcgat cacggcatga tgctgcttat tttttcggca ttcatgtggc
     4081 gttatttcgg caaagttgca ggccttatcg cccttattat ttttatggtt tttgcatttc
     4141 ccagagtaat gattggcgca cactggttta ctgacatcat tgtcggttcg atgaccgtga
     4201 tattgatcgg tttgccctgg gtgttgctga cgccattaag tgatcgatta atcacctttt
     4261 ttgacaaatc actaccagga aaaaacaaac atttccaaaa caaataactc acagtaatta
     4321 acatcatcag ggttattttt atagtgagga taatcctgat gatgcgcacc gtgctttcat
     4381 ctatcgaacg caaaaatcat tctctaagta aatgaatgga ttgcatgcgt ttcactcaat
     4441 tgtactttaa ttgaccaacc ccgcttatta actttctgta tcactttttc ttataaaaaa
     4501 tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa tctcgaactc gttttgcctc
     4561 ggcggtagat tatcctcaca gcatataatt ttgtgcgtta gtccacagat ttggccttaa
     4621 ggaattgttt caacatgccc aggtaattag tctcgtgtcg cttggcattt ttttataacg
     4681 atatttgtcg ttaaggactt caagggaaaa caaacaacat ggtcaaatct caaccgattt
     4741 tgagatatat cttgcgcggg attcccgcga ttgcagtagc ggttctgctt tctgcatgta
     4801 gtgcaaataa caccgcaaag aatatgcatc ctgagacacg tgcagtgggt agtgaaacat
     4861 catcactgca agcttctcag gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat
     4921 cgcgaattat ggatcagtat gctgactgga aaggcgtacg ttatcgtctg ggcggcagca
     4981 ctaaaaaagg tatcgattgt tctggtttcg tacagcgtac attccgtgag caatttggct
     5041 tagaacttcc gcgttcgact tacgaacagc aggaaatggg taaatctgtt tcccgcagta
     5101 atttgcgtac gggtgattta gttctgttcc gtgccggttc aacgggacgc catgtcggta
     5161 tttatatcgg caacaatcag tttgtccatg cttccaccag cagtggtgtt attatttcca
     5221 gcatgaatga accgtactgg aagaagcgtt acaacgaagc acgccgggtt ctcagccgca
     5281 gctaataaac cgtttggatg caatcccttg gctatcctga cgagttaact gaaagcactg
     5341 cttaggcagt gcttttttgt tttcattcat cagagaaaat gatgtttccg cgtcttgatc
     5401 caggctatag tccggtcatt gttatctttt aaatgttgtc gtaatttcag gaaattaacg
     5461 gaatcatgtt catacgcgct cccaattctg gacgtaagct cctgcttacc tgcattgtag
     5521 caggcgtgat gattgcgata ctggttagct gccttcagtt tttagtggcc tggcataagc
     5581 acgaagtcaa atacgacaca ctgattatcg acgtacaaaa gtatctcgat acctattttg
     5641 ccgacctgaa atccactact gaccggcttc agccgctgac cttagatacc tgccagcagg
     5701 ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa tgtccgtacg tttgtgctgg
     5761 tgaaagataa aaaaacattc tgttcatctg cgactggtga gatggacatt ccactcaatg
     5821 aattgattcc ggcgctcgac attaataaaa acgtcgatat ggcgatctta cccggcacgc
     5881 cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg caaccctttg ctgaaaaata
     5941 gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc tctcttttat agttcacggc
     6001 aggaagatta cgatggcgtc gccctcatta ttggcaatac tgcgctatct accttttctt
     6061 cacgtttgat gaacgttaat gaattaaccg acatgccggt ccgtgaaact aaaattgcgg
     6121 gcattcctct gaccgttcgg ctttatgcgg atgactggac atggaacgat gtgtggtacg
     6181 catttttact gggcggcatg agtggaactg tcgttggcct gctttgctat tacctgatga
     6241 gcgtacgtat gcgccccgac agagaaatca tgaccgccat caagcgcgaa caattttacg
     6301 tggcgtatca accggtggtg gatacacaag ctttgcgggt aacgggcctg gaagtactgc
     6361 tacgctggcg gcatcctgta gcgggagaaa ttcccccgga tgccttcatt aactttgccg
     6421 aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt tgagttaatt gcccgcgatg
     6481 ccgcagaatt agaaaaagta ctgccggtag gcgtcaaatt tggcattaac attgcgccgg
     6541 accatctgca cagcgaaagc tttaaagcag atatccagaa actgctcact tccctgcccg
     6601 cacaccattt ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag
     6661 ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt gatgacttcg
     6721 gcaccgggca cagcgcgctt atctatcttg agcgttttac gctcgattat ctgaaaattg
     6781 accgtggatt tatcaacgcc atcggtacgg aaacgatcac ttcacccgta cttgacgcgg
     6841 tgctgacgct ggcgaaacgt ctcaatatgc tgacagttac tgaaggggtc gaaacgccgg
     6901 aacaagcgcg atggctaagc gaacgcggcg ttaatttcat gcaaggctac tggattagtc
     6961 acccgttacc gctggacgat tttgttcgct ggctgaagaa accgtatacg ccgcagtggt
     7021 aaggtgtgct tacgtccctt attattcata gtgaaagcat gccggattgc ggctaatgat
     7081 gagtaaaagg aaatccgttg cagatgattg tgcgcatact gctgctgttt atcgctctgt
     7141 tcaccttcgg tgcgcaggcg caggctatca aggaaagcta tacctttgcc gtgctgggcg
     7201 aaccccggta cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc gcgccaaaag
     7261 gtgggcagat aacgttgtca gccctcggta ccttcgataa tttcaaccgc tatgcactgc
     7321 gcggcaaccc gggcgcacgc accgagcagc tgtacgacac gttatttacg acttccgatg
     7381 acgaaccagg cagttattac ccgctgattg ctgaaagtgc acgctatgct gacgattatt
     7441 cctgggtgga ggtcgctatt aatccgcgcg cccgttttca tgatggttct cccattactg
     7501 cccgcgatgt agagtttact tttcaaaaat ttctgaccga aggcgtgccg caatttcgtc
     7561 tggtctacaa aggcaccacc gtcaaagcca ttgcgccgtt aaccgtgcgc attgagttag
     7621 ctaaacccgg caaagaagat atgttgagtc tgttttcgct gccggtattt ccagaaaagt
     7681 actggaagga tcacaaactt agcgacccgc tcgccacgcc tccgcttgcc agtggtccgt
     7741 accacgttac gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg aaagattact
     7801 gggcagcaaa cttaccggta aaccgtggac gctggaattt cgacaccatt cgctacgatt
     7861 attacctcga tgataatgtc gcctttgaag cgtttaaagc aggtgccttt gatttgcgta
     7921 tggaaaacga cgctaaaaac tgggccacgc gttataccgg taaaaatttc gataaaaaat
     7981 acatcatcaa agatgagcaa aagaacgaat cagcccagga tacgcgctgg ctggcgttta
     8041 atatccaacg tgcggtattc agcgatcgcc gggtgcggga agcaatcact ctcgcctttg
     8101 actttgaatg gatgaacaag gcgttgtttt acaacgcctg gagtcgcacg aacagttatt
     8161 ttcaaaatac cgaatacgcg gccagaaatt accccgacgc cgcggagctg gtgcttctgg
     8221 caccaatgaa aaaagatcta ccgccagaag tcttcacaca aatctaccag ccgccggtat
     8281 ctaaaggcga tggctacgat cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaag
     8341 cgggctgggt gctgaagggt cagcaacgcg ttaatgccac aacgggtcag ccgctcagct
     8401 ttgaattatt gcttcccgca agcagcaata gtcagtgggt attgccgttc cagcacagcc
     8461 tgcaacggct gggtatcaat atggacattc gcaaggtgga taactcgcaa atcactaacc
     8521 gcatgcgcaa tcgcgactat gacatgatgc cgcgactatg gcgggcgatg ccgtggccca
     8581 gttccgattt acagatttcc tggtcatcgg aatatatcaa ttccacttat aatgcccccg
     8641 gcgtgcaaag cccggttatc gactcactga tcaaccaaat tattgccgcg cagggaaata
     8701 aagaaaaatt actgccgttg gggcgagcac tggatcgcgt attaacgtgg aattattaca
     8761 tgctgccaat gtggtacatg gcggaagacc gtctcgcctg gtgggataaa ttctcccaac
     8821 ccgctgtacg ccctgtttac agcctgggta tcgatacctg gtggtatgac gttaataaag
     8881 cggccaaact gccgtcagcc agacaacagg gagagtagat gggcgcttac ctgattcgcc
     8941 gtctgttgct ggtgatccca acgttatggg ctattatcac tatcaacttt ttcatcgtgc
     9001 aaattgcgcc tggcggtccg gtcgatcagg ccatcgccgc cattgagttt ggtaatgccg
     9061 gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa acgggtgtcg
     9121 gcaatatcag cgacagtaat taccgtggcg gacgcggatt agatccagaa gtgatcgctg
     9181 agatcactca tcgctacggt ttcgataagc cgatccacga acgttacttc aaaatgctct
     9241 gggactacat ccgctttgat tttggtgata gcctgtttcg cagcgcctcg gtgctgacgc
     9301 tgattaaaga cagtctgccg gtttccatca ccctcggatt gtggagcacg ctgattatct
     9361 atctggtgtc gattccgtta ggcattcgca aagctgttta taatgggagt cgctttgacg
     9421 tctggagtag cgcatttatc atcatcggct acgccattcc ggcctttttg tttgccatcc
     9481 tgctgattgt cttcttcgcg ggcggcagct atttcgacct gttccctcta cgcggcctgg
     9541 tttccgctaa ctttgattcg ctgccgtggt atcagaaaat caccgattat ctgtggcata
     9601 tcacgctacc ggtgctggcg acagtgattg gtggctttgc ggcgctgacc atgctgacca
     9661 aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg
     9721 taagtgagaa aaacattctc tggaaacata tgttccgcaa cgccatgctg ctggtgattg
     9781 ccggttttcc ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg attgaggtga
     9841 tgttttcact caatggtctt ggcttactgg gctacgaagc gaccgtctcg cgcgattatc
     9901 ctgtaatgtt tggtaccttg tatattttca ccctgattgg cctgctgctg aatattgtca
     9961 gtgatatcag ctatacgctg gttgatccgc gtatagattt tgagggacgt taatgtcgcg
    10021 actcagcccc gtcaatcagg cccgttgggc gcgttttcgt cataaccgtc gcggctactg
    10081 gtcgttatgg attttcctcg ttttgtttgg tttgagtttg tgttctgaac ttatcgctaa
    10141 cgataaaccg ttgctggtgc gttatgacgg cagttggtat ttcccgttgt tgaaaaacta
    10201 cagcgaaagc gattttggcg gcccgctggc aagtcaggct gattatcagg acccgtggct
    10261 gaaacaacgg ctggaaaata acggctgggt actgtgggca ccgattcgct ttggtgctac
    10321 cagtatcaac tttgctaccg ataagccctt cccttctcca ccctcccggc aaaactggct
    10381 gggaacggat gcaaacggcg gcgatgtgct ggcacgcatt ctctatggca cgcggatctc
    10441 ggttctgttt ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc tggcgggggc
    10501 gctacaaggc tattacggcg gtaaagtcga tctctggggg caacgcttta ttgaagtatg
    10561 gtcggggatg ccgacgctgt ttttgattat tttactttcc agcgtcgtac agcctaactt
    10621 ctggtggctg ctggcaatta ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg
    10681 ggcggagttt ttacgtactc gtaatttcga ctacattcgt gcggcacagg cgcttggcgt
    10741 cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat gccatggtcg cgaccctcac
    10801 ctttttaccg tttattttat gtagttcgat aaccaccctg acctcgctcg atttcctcgg
    10861 cttcggtcta ccgctcggtt caccgtcact cggtgaactg ctgttacaag ggaaaaataa
    10921 ccttcaggcc ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt
    10981 gctgatcttt attggtgaag ccgtccgcga cgcatttgat cctaataagg cggtgtagca
    11041 tgacgcaaac tctgttagcg attgaaaatt tgtcggtggg ttttcgccat cagcaaaccg
    11101 tacgtacagt agtcaatgat gtttcactac agattgaggc tggcgaaacg ctggcgctgg
    11161 tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt
    11221 ccccgccggt tgaatatctc tccggcgata ttcgttttca tggcgaatcg ctgcttcatg
    11281 ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt tttcaggaac
    11341 cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca gctttatgaa gtgctttcac
    11401 tccaccgcgg gatgcgtcgg gaagcggctc gtggcgaaat tcttaactgc cttgatcgcg
    11461 tcggtatccg ccaggcggca aaacggctga cagattatcc gcatcagctc tccggcggcg
    11521 aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg accggaatta ttaattgccg
    11581 atgaaccgac caccgcactg gacgtctctg tccaggcgca gattttacag ctgttgcgcg
    11641 aactgcaagg cgagctgaat atgggcatgc tgtttattac tcataacctc agcattgtca
    11701 gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg
    11761 ccgctacgct atttgcctca cccactcatc cttacacaca aaagctactc aacagtgaac
    11821 cgtcaggcga tccggtaccg ttgccagaac ctgcctcaac gttgctggat gttgaacagc
    11881 ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg cattgtggat cataatgtgg
    11941 tggtgaaaaa catcagtttt acgctacggg cgggtgaaac actgggttta gtgggcgagt
    12001 ctggttccgg gaaaagcacg acgggactgg cgctgctgcg actgattacc tctcagggca
    12061 gcatcgtctt tgacggtcag ccactgcaag atttaaatcg ccgccagctg ttacctactc
    12121 gtcatcgtat tcaggtggta tttcaggatc caaactcctc gctcaaccca cgactcaacg
    12181 ttttgcagat tattgaggaa ggcttacggg ttcaccagcc gacgctttct gccgcacaac
    12241 gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt agatcctgaa acacgccacc
    12301 gttatccggc ggagttctct ggtggtcagc gacaacgtat tgcgattgcc agggcattaa
    12361 ttcttaagcc ctcgctgatc atacttgatg aaccgacatc atcactcgac aaaacggtac
    12421 aggcgcaaat attgacgctt ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt
    12481 ttatcagcca cgatttgcac gttgtccgcg cgttatgtca tcaggttatc gtactgcgac
    12541 aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt tgccacaccg cagcaggagt
    12601 atacgcgtca gttactggcg ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt
    12661 tcggcaattg ccacaccaaa atttttcaat cggcatgttg cagcaaactc gtcctggcga
    12721 tttacaaaca ggcacggctc accttcacac tccaacaccg aacatggaac ttcgatgtcg
    12781 cttaacgcct ggcttagttt atacatcacc gaccatgcat tatcaccatc cgggctaagc
    12841 aatttaagcg ccaccaggct gttgtcaccg cacggtccat tttgcggaat cggttcaacc
    12901 ttcgaacctg cgaaaccagc agaccagcga tagttctgcg gcagtcgatg gacgattgag
    12961 agttgtaatg aagtcactta ttttccccgg aagtacattc acttcacaat ttgtttacat
    13021 caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt gatgcctcgc
    13081 ctgcgtggcc tctatggctc tgatttaagt aatatctgcg ggcgtggtca cgtttgcggg
    13141 gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg atgtgacttt
    13201 ttacataaat tgattttaca taaaataaac atatatcggg gaaatgatag atttgtggtt
    13261 gacatgcatc aacaaaggaa gccttttacc ttcctcgttg tgcaatagat caccgttttt
    13321 tcggccgact ggcgtacaga cagaagagaa tggagctggt tgcgcagaat gcaattgacc
    13381 aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag caatgcgcca acaattgccc
    13441 ctatgccaaa acggaaggtt cctgccagcg aagatgccgt tcccgccata tggggaaact
    13501 catcaagaat gaccgccatc gcattggatg acaccatcga cacgcagccc acaaacgccg
    13561 caacgccaac caccagcgac cagaatccca gccccagcgg cgcactgatg accatccacg
    13621 cagccataat aaattgtatc cacaaccccg agcggaacat atttaacgcg ccaatgcggc
    13681 ggacgaagcg gctgttaaag atggtcatca cgaacagaaa aacaatgttt agcgcaaagt
    13741 aataaccaaa gttttccggc gcgacgtggt taatttcaat ataaacaaac ggtccggcgc
    13801 ttaagaatga gaacatcccg gcaaagctga aaccactggc aagcatgtag ctcaggacac
    13861 gtttatggcg gaacagcgcc gcaaagttac caatagtggt acgaatgtga aatggctgac
    13921 gacgctccgg tggtaaggtt tctttaatca ggaagaaaat cattgccgaa gccagaatcg
    13981 ccgctaatgc caggatccag aagatgtaat gccagctcag ccacaccagc acccagccgc
    14041 caactatcgg tgccatcagc agtgcaatgg tcgtcaccag catgacaaac gacatcatcc
    14101 gcgagaactc ttctttcggg taaatatcac gcatcaaagc gttgataacc acgctggccg
    14161 cagccgcagc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg gtttgtgcca
    14221 acgcacacgc caccgcggcg gcggcaaaca ccagcgtccc gccaagaacc accggcttac
    14281 gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag cccagaatat
    14341 aagtactgag ggtcatctgc gtactgcccg ccgatacgcc aaactgcgct gaaattaccg
    14401 gtagcgcggg cagatacata tcaatcgaca gcggcatcaa catggccagc aggccaagga
    14461 taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat
    14521 tgaaaagtta gacgacgctg gcaatttctt cttcagttaa cggacgatat tcaccggggg
    14581 ctaaatcagc atccagcgta ataccgccaa tacgttcacg atgcagctca accacgtggt
    14641 tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg atggtcagac
    14701 gaacctgcgt tggggtaatc acttcaagca ccgccggctt agtgagatct ttttcgttat
    14761 gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacaggt gattccagtg
    14821 tcaccagata ggtcttctcg caatgatggc gcggagaagt aatgcggtgc gaccactgac
    14881 catcatcagt catcagcacc agaccggtgg tatcgatatc cagacgtcct gcggcgtgca
    14941 gtttccacgc taccggttca tccagaaaat agagcaccgt cgggtgatcc gggtcgtccg
    15001 tggagcaaac atagccctga ggcttattga gcatgaagta acgtggaccg tgttgctgcg
    15061 ccagcgggtt gccatcgtaa gcgacatcat gttcagggag cagtttgaac gctgcattac
    15121 gaacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg gcaatagcac
    15181 ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg attttgcctg
    15241 taaaaaacgg aggtcgggta atgacccgaa aatctgaact gttgtctgcc cagtatagcg
    15301 gtctaataac ttccctcaag ggaaaaagaa ccgtggcata ctattagcag aataatctac
    15361 ctacgcgaaa ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg
    15421 ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt
    15481 aaaagcctgg tgatagcgga actggcacgg ctggcgcgtg gtcgcgtact ggtgctggca
    15541 cacgttaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc
    15601 gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtggt atttggcagc
    15661 gttcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc
    15721 gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac
    15781 ctgactaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg
    15841 ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc
    15901 cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg
    15961 acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca
    16021 cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc
    16081 attacgccac atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg
    16141 attttcgccg ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat
    16201 gctgcactga ttactggcga caaccccggc gctgagcgcg atgtgttaat tgaagatttt
    16261 aaagcccagc gttttcgcta tctggtcaac gtcgcagtac tgaccaccgg atttgacgcc
    16321 ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct ttaccaacaa
    16381 attgtcgggc gcggtctgcg tctcgctccg ggcaagactg attgtttaat tcttgattat
    16441 gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg caaaagtgac
    16501 aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa
    16561 acgaccgccg acgggacatt gattgaacac tttggtcgcc gctgtcaggg atggtttgaa
    16621 gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa
    16681 tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt
    16741 gatccggatg atatgttaaa agcggcgcta cgactgaaag atgcgctggt attacgctgt
    16801 agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat
    16861 tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcgt
    16921 accgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc
    16981 tggatcaccg ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc
    17041 gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga ttacgaaggt
    17101 cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg
    17161 attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg
    17221 ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt
    17281 acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc
    17341 aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat
    17401 gaacatgcaa gctaaagctg aattctacag cgaagttcta accatcgttg ttgacggtaa
    17461 agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca
    17521 catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg
    17581 ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa
    17641 ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg ccagacgtcc
    17701 ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg
    17761 ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac
    17821 cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct
    17881 cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg cttacgcctg
    17941 ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac
    18001 agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg
    18061 cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt
    18121 cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca
    18181 ggaaagtgag atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg
    18241 caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt
    18301 taacgcgcat actgctcagg ttgctcagaa ctgccaccag caaatactcc accgccagat
    18361 aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt
    18421 cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct
    18481 gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt
    18541 tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca
    18601 gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca
    18661 gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc
    18721 aaccaccgcc agttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta
    18781 acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg
    18841 ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac agtaatgaac
    18901 aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg
    18961 acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc
    19021 cggcccaacg ccaggcaatt gccaactctt ttgccagcgc cttacagtcc tctatcaacg
    19081 aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac
    19141 cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg
    19201 ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct
    19261 ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctagt gttcgccacc
    19321 tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt
    19381 ttgtccgtca ttctggcgac ggcgggaatg acgctattac tgatcgatag cgaagtcttt
    19441 actcgtttcc atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa
    19501 aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt
    19561 gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc
    19621 ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat
    19681 atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt
    19741 tcgtacccga tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag
    19801 tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta
    19861 agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat
    19921 ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa
    19981 aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc
    20041 ctgttctatg gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacgcctgcg
    20101 gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc atcagatggc
    20161 tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc
    20221 acccaatctg acgatcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa
    20281 gaagataacc gctggttctc gtgggtttct ttcaatggca ctaacattga cgacagcaat
    20341 cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaaa
    20401 cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggtggt gattatcact
    20461 gccggtcggg gtattccact gagcgaagag gaagaaacct ttgactggtc ccacggtcat
    20521 ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgctctg
    20581 actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctgcatgt cagcacacct
    20641 gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtt
    20701 actgctgcgg ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac
    20761 aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca
    20821 cagttaagtt tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta
    20881 attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag
    20941 cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc
    21001 ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga aaaaaccaac ccttacggtt
    21061 ggttttttta tatctgcaat taattcgata aacagaccgt gacacatcac agcctgttta
    21121 ttttctgtta tcagaacgtc cagaccacac ccgcctgagt attccacggc gattctaccc
    21181 ctgcgccatt gccatagctg actgacaaat gaccgcttaa cgtcggggta aacgatgacc
    21241 ttatcccagc ctgataaacg ccactggttc cagccacatc attattaaat ttgccatcgt
    21301 cattaacttt cacctggtta gaatcggcgt attcctgacg cacggcggct ttcagccagg
    21361 gttccagcgt cgtaccgttt tgcaggtcca tgtgatagct taccgccgtt cccgcttcag
    21421 cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc gtttgataac gtgtagtcct
    21481 gaccatctgt ggtaaagccg gtaaaggcca gatagggtct aacactccac aatccgtcaa
    21541 cccaacggaa cccgctctca acatgagcac ccgcgccgtt actattgtaa tcgccaaacg
    21601 ctgttgcccc attactcatc ttgccatgga tggtgttggc aaaacggtca actttcacca
    21661 ccccatcaac ataggcaccg ttctgatgct cccaaccggc ataagccccc agggtatagc
    21721 tatcgatatt accttttccg ccgcgatcaa aaccaatatc agaatgagag taaccaaaga
    21781 acaagccgcg aattgtactg ctttcttcac gggagaaacg gctatcgata ccgagcgtca
    21841 ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt
    21901 taattgccga actccacatc accgtatcgt aactaacgcc ttttacgcta ccaagacgct
    21961 cacgcacggt gtccagttct gcatcaaata ccagcggttg tgcggctgcc atgttcagca
    22021 catcagtggt tgaaggggta atttgcgcgc gattctctgc gaggctccag ctatggttgc
    22081 cattatccag caaggtatat tcatacgtac cgatatcaac aacgcctccg gcattgccca
    22141 acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt aaggctatct cctgctgccg
    22201 ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc tgttgcctga ccggtgacgt
    22261 tgagctgatc gctctgatga ttggccatat cggtacgcat ataaaaattg ccgtttcccg
    22321 acagggtatt ggtggtcaac gtaatgaaat tttccgcact tgccgtcaca gatgaacgag
    22381 tcactggctc agccatcata atcacatttg cgccggacat cgcaaggttg gctaagttga
    22441 ggcgagtgtt ttggttcgtc gtagtgatat cggcaatgat ggtccatgcg ctattttcga
    22501 gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt tctaaaagaa gcgccctcag
    22561 aaaccactac cttacccacc aacatagcgg gtgcataatt tatttgcggc ttcatgacct
    22621 caagaatgcc atcattccct ctgactgaca cgttaaccat tgtgccagcc cagacattag
    22681 cgcggccatt gttgattacc atattttcag agatggcttt tgaggaatac gtgatacttc
    22741 cattctggtc tgatcgaccg agatcatata ccgcatttgc ctgcactttc gtaccagtat
    22801 cctttcccag cgattgcata gtggcatgct catcaaggat agtgtcaatg gcctgcgtgt
    22861 cctccataac aatgagcccg gaaccatcat ccagttcata attttttgac acaccatcct
    22921 ttatactaaa ttggccttta ctgtttgtac cactcacttc cagcgcattc gttgagacaa
    22981 ttaatttccc gcctgctttt ttatcaacgc cagttgcagt cccgccatcc ataacctcca
    23041 gtaagccgcc actatccaca gtagtattat aagcgaagtc attttcttca actcgcaaac
    23101 ttccgccgtt ttccaacaac atattattag caataccatt cttgatatcg aactgaccga
    23161 gacggtttgt tccgaatacc tccatggccc gcgtggttgt tttaatagca ccgcctgttt
    23221 tctgatctac cgcaagcgca aatcccccct ctttaacctc cagtagtcct cccttttcga
    23281 tagcggcaaa aacggccccc ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg
    23341 tatcattcgc tgggccataa acctgtagca caccattatt gagggtagtc tggtttgccg
    23401 cgccacccat ttgaacaatt tgataaccat catttaatac tgagtattct gcggtaccat
    23461 tcatttcaat gtattgatac cctccgttaa tttcagtatt actacttaca ccaaattctt
    23521 ttatatgctg ttctccgcca ggattgattt tcgtattatt ggcggtccca taaaccagct
    23581 ggatatcttt ttccaggata acaccatcaa ctgtctcacc atactcaact gttgatgcca
    23641 atgctatggg tgaatataac gctgtagcca ccagaacaga tattgcggaa cgacaaagag
    23701 aaacagaacc agattgatgc attgagcttt catcctatga aattaattgc agttaaaagc
    23761 attgggtaca gaaaataccc atagctccat acccggagtc agtttttaaa aactgtttaa
    23821 agaaatgcac aagtattgtg attgattttt tagttgtttt tcttgatgag aagctgatgc
    23881 aaaattccgt ctttataata aaaatgatgc caaagcgaac gacaaggttg tagttttcac
    23941 tacatgtcca tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac
    24001 gctcaaacat aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt
    24061 ttcaggtgat gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg
    24121 agcttgatcc tggctttgaa gtggtcgccg aagcgggcga aggcgcgagc gcgatcgatc
    24181 tggcgaatag actggatatc gacgtgatct tgctggatct caatatgaaa ggtatgagtg
    24241 gcctggatac cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt attatcctga
    24301 ccgtatccga tgcctccagc gatgtctttg cgctgataga cgcaggcgca gacggttatc
    24361 tgttgaaaga cagcgacccg gaagtattgc tggaagcgat tcgtaccgga gcgaaaggca
    24421 gcaaagtctt tagcgaacgc gtcaatcagt acttacgtga acgtgaaatg tttggcgcgg
    24481 aagaagatcc cttcagcgtg ctgacggagc gcgagctgga tgttctgcac gagctggcac
    24541 aggggctgtc aaataaacag attgcatcgg tgttgaatat ttccgagcag acagtaaaag
    24601 tacatattcg caatctgctg cgtaaactca atgtccgctc acgcgtggcg gccaccattc
    24661 tgttcctgca acaacgcggg gcacaataaa aatagcccga tggatttatc atcgggctga
    24721 gatttatgac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta cgtggatcga
    24781 tcaatttatt gaatttacac aattttgtag gccggataag gcgttcacgc cgcatccggc
    24841 ataaactaag cgcactttgt catcagtcta gcccgatggc atcaccatcg ggcctctttt
    24901 tatttactct cctgcggcga caaatgttgc atcgcctgcg cgatactacg ttcaatcacc
    24961 gcacggcgag tatcgttggc aggtaagagt ttcaacatca tctcccacgc ggcaacggct
    25021 tcgccaaatc gctgctgctc aaaggcatta aacgcataca tgcttagcac acggatattg
    25081 ctatggtccg ttctcaccag ctgacgtagc agttcaccgc cgaggcggtt gtcgttggga
    25141 tcagatgaac gagtcaacgc ttcagcgtat cccagtgcgg catcgctgtt cttcggatcg
    25201 aggcgatacg cagtagcgta tgcatcggtg gcgatactgg cgttacccag cgccatgcca
    25261 acgcggccca acataatcca gccttctata tctcccggat ttttttgcag ttgagtacgc
    25321 atccccagcg caaggcgcga catctcttct tcgttgagcg gatcggcttt cgggtccagc
    25381 gccctgtcca gcaacgccgg agcctgtgcc gtggcctgca gccagatttt cacctgctga
    25441 taattgccag tctggtagta gctgacgcca gccacaatta acgccaccac aatacccggc
    25501 agataaacaa catatccggc acgcttacct tccggcacgc tttgttcagg aaacgcttcc
    25561 ggcaccacgc gtacccgacg ccgcgagcgg gcgtatatga cccaaccgcc aatgccaata
    25621 gccactactg gcagcaccca cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg
    25681 acgaagttgc cgtaacgcgc caccatataa tcgacaatct cttttttact tttaccttcc
    25741 tgcatcagtt catacacttt ctgacgcagg tcggtggcaa tcatcgagtt ggaatcggca
    25801 atgctgttgt tctggcattt cgggcagcgc agttcttcag tgagctgacg gaactgttgt
    25861 tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg ccagcgctga gccggagatc
    25921 atcagcatca gcacgcccaa taaaaacctc attgtgcggc ctccttactg tatttctccc
    25981 acagcggctt gatctcttct tcccagacgc gaggattgag atcgcccgca tggcgatagc
    26041 gaatgatacc gttgccgtca ataaggaagg tttctggcgc gccatagaca ccgagatcca
    26101 gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta aggattgccc agctctttca
    26161 gccagctgat tgccttctgg cgatcgtctt tatagttcat gccgaccacg cgaatgccct
    26221 gcgctgaaag ctgattcaga tattgatgtt ccgcacggca ggtcggacac caagtcgccc
    26281 agacgttaag cagtactggt ttgccctgag tcagcacatc cgcctgataa aactgccccg
    26341 gattgtccag tgattcgaga cgaaacttcg gcacaggctt gccaatgagc gccgattcca
    26401 gattggtcgg atcatcccct tcggcattac gcgccagctg ccacagcagc gccgcggcaa
    26461 tcgccaggaa gataatcaac ggaattaaca atactttgcg cttcatacgg cctccggcgc
    26521 agttttttgc ggactcacgc gcttacgata gcgaggatca aacagacaca gcagtccgcc
    26581 caacgccatc atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg
    26641 cacggcccac gcgccgtttt ccagctcttc accgagcgcg gcgtacaggt cacgcgtgat
    26701 gccgccgtca attgccgctt cggtcatcat cgacccggca gtgttgtaat aacgtttttc
    26761 cgcatacagc accgtttccg gtttaccctc tcgcgttacg ccaatagtcg ccacaccgcc
    26821 gcgccagttc gggccagtca cctctttgac atcacggaag gtgaagcgat attcatgaat
    26881 atcgacgcta tcgccggact tcatgcgcac atcacgctca acgctataat tctggctaaa
    26941 ggcaatgcca acaattgtca ctgccagccc caggtgagcc gccaccatcc cccaataact
    27001 gaaggtggtt ttcgtgccgc gtgaaatacg cagcgcagct tccgcagttg ccagcaccgc
    27061 aatccagcag gccattgcca ggccgagcac cgtcatcgcc acaactttgc tttcgaacag
    27121 ccacggcaac agcagagaca gcaccagcgt agagatgaag gcgataatca ataaattgcg
    27181 gattttacgt gggcgatcgc gcccccagcg caccagagga ccgacaccaa gcagtagcgc
    27241 aaacggcacc atcagccagg taaacatggt gttgaagaac ggttcgccaa tcgaaatact
    27301 gcccagtcca agttgcttgt gcaccagcgg cagcagcgtc cccagcaaca ccaccagcat
    27361 cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat tcccgcgacc acagcgcatt
    27421 gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat
    27481 caccagcacc ataaaggcga ggataaacat accgcgcgcc ggatcagacg caaacgcgtg
    27541 taccgatacc agcacgccgg aacgcacgag gaaagtcccc aacagacaca acgagaaggc
    27601 actgattgcc agcagtaatg tccacgcttt gaagctggcg cgttgttcag tgaccgccag
    27661 tgagtgcatc agtgcagtcc ccaccagcca cggcataaac gaggcgtttt ctaccggatc
    27721 ccagaaccac cagccacccc agccgagttc gtaataggcc catgcggaac cgagcacaat
    27781 gccgagcgtt aggaaaatcc acgccgccag cgtccacggg cgagtaaaac gcgcataagt
    27841 gctgtccaga cgcccgctca gcaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc
    27901 cacgtacccc atataaagca gaggcggatg gaagatcagc cccggatcct gtaacagcgg
    27961 gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg cgtgagaacg ggttagaggt
    28021 aaagagaatg aacagcaaaa agccgacgct gaccatcccc attatcgcca gtacacgggc
    28081 cacaatatcc agcggaatac gctgactaaa aatcgccacc gcaaaggtcc agccgctcat
    28141 cagcagcacc cacagcagaa gcgaaccttc atgcgcgccc caagtagccg ccacgcgata
    28201 ccacaccgga agctgggtat tggagttgct ggcaacatag gtgacggtaa agtcgttgac
    28261 cacgaaagca ttgaccagca ccagaaatgc gccagccaca gacataaaca gcagccaggc
    28321 aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc gccacgcccc atagcggata
    28381 cacggacagc agcagcgcaa tccctaacgc caagcacagc agcccgtttc caatttccgg
    28441 catcatgatg ctgggtcctt ataaacactc gccgggcgac ggtggttagc ttccatcgct
    28501 ttctcaactt ctggcggcgt gtagttttcg tcgtgtttcg ccagcacttc tttcgcgagg
    28561 atatgattgc ctttttccag ctcgccctgc accacaacgc cctgcccttc acggaacaga
    28621 tccggcaaaa tgccttcgta agagacatcc actgagcctt cagcatcgta aatggtgaag
    28681 gtcactttca gcgaattggg atcgcgctgc acgctacccg gcatcaccat cccgccaacg
    28741 cgcagacgct gaccgacttc cggcatctgc tgagtttcac gcttgccgta gagaatttcc
    28801 cccggcgtat aaaagagatc gatattcgag cgcagcgcat atagcaccag accgatagtc
    28861 agcgctagcc ctgccaacac ggcacaggca atccacaagc ggtttttacg gcgaatattc
    28921 atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc acgcgcccgc tgttgcgcca
    28981 caccacgcag aattgcgcga tgttgcatca ccgagtgcac gaccaaaacc accagcggaa
    29041 taacggtcat caccaccgcc agccagacaa aaaaggcgta accgcccatt gcgaaaaatt
    29101 cattccagga agcaaatgca ggggtcattt acggcctctt ttcagtatca gttcactcac
    29161 ccacggacgg cgtttttcca tcagcaaaat caaattacgc atccgcatca gcgtcagcgt
    29221 ggcagacagg agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca tcgccggatc
    29281 gatactttgc tgcatccgcg ttgatccctg atgcagggtg ttccaccact ccacggagta
    29341 atgaataatc ggcagattca ccacgccaat cagcaccagg atacctgccg cacggcccgc
    29401 cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca cccacataca aaaacagcag
    29461 caccagttca gaagtcagac gtgcatccca tacccaccag gtgccccaca tcggttttcc
    29521 ccatgcagag ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc
    29581 cgccaccgcc aggttggcca ttttcatctg ccagacaagg ccaataaacg ccgccaccgc
    29641 cattgatgca taaatgccca tcgaccagat cgccgcaggc acatgcaggt agataatgcg
    29701 gtagctattt ccctgctgat aatcagccgg agcaaagccg aatccccaga tccagccgac
    29761 ggtaagcacg accacactgg caattgccag ccacggtata aaccagccac agatttgata
    29821 cagccgtggt gggatcgcca gttgatgcag tgttttccac atagtttcga taccagactc
    29881 gaacaaaaat cagtaatcca gcgttattga atgctgattc gtaacgctgc cgccgtcgca
    29941 aaaggactta atgtcgcggt gcctgccagc aacgcgccta aaattgccag atacccatca
    30001 acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg caaagatgag taatgggata
    30061 gtcagcggta acaccagtat gctgagcagc acaccaccgc gcttaagtcc cactgtcagc
    30121 gccacgcccg gtgcaccgag aaagccaagc gtaggcgttc ccagcagcag cgtcagcgcc
    30181 atcacttgcc agccataaac atccattccc agtagcattg ctaccagtgg cgaaaggatg
    30241 agtaacggca gaccggttac catccagtgc gccatcacct tcgccagcac aacggcgggc
    30301 aagggtaacg gcaacaacat caattgttca agactgccgt cctgcaaatc gtcacggaac
    30361 agtcgttcca gcgccagcaa ggatgaaagc agcgcagcaa cccagataat gcccggtgca
    30421 atacgcgcca gcagttgcgg ctccggaccg atactgagcg gaaaaagggt aattacaatc
    30481 aggaagaacc acagcgggtt ggcgatttcg gcgctatggc gaaacgctac gcgcagttca
    30541 agacggaaaa tacgccagaa catcatgcgg ccctcgtttg cgtcagtgaa atgcggcgaa
    30601 ttttactttc agcaacgttg agcggctggt gggtagtcag aatcacaatc cccccctgct
    30661 ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc gttaacgtca atcgcggtaa
    30721 aaggctcgtc gaggatccat aatgtggcac gggtcaacca cagacgcgct aaagcgacgc
    30781 ggcgttgttg cccggcagaa agttgattta ccggaatatc ttcgaatccg gcaagcccgg
    30841 cctgcgccaa ggcttccaga cattgtgcgg catcgccatc gcgatgataa aagtgcagat
    30901 tttctaacgc cgtcagccgg gttttgatcc ccggctgatg gcctatccat aacagggttt
    30961 gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacc tcgccagcgt
    31021 cagggcgaga caaccccgtc agcaaacgga gaagcgttgt cttccccgcg ccgttgctac
    31081 cggtgatttg tacccactct cctgcgttca gcgtaaatga caagccacta aataaggttc
    31141 gttcatcccg ctcacaaagt aactctctgg cttcaagcat acccacgcaa taaccctgtt
    31201 aaaaacctgg ctcgacttca cgcatatcgg gcagcttgtg cgcgatccct ttatggcaat
    31261 caatacaggt ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca accgatttct
    31321 gggcggttgt atccatatac tcgaagttgt gacagttacg gcactcctgc gagttattgt
    31381 ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg atgagcttca aatttctgcg
    31441 gcgtgtcaat aacgccaaaa attttaccat acagctcttt acttgctttg agcttgcgta
    31501 tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg acaggtcgca cggacgccgc
    31561 tacggttgtt gtagtgcacg gaatccatgt attcctgata caccgtgttg cgcatttcgt
    31621 ggcagctaat gcagaactct tcggtattgg ctttttccat cccggtgtta aagccccccc
    31681 agaagacgat gccgccaaca aaaccgatca acagcagcgt ccccagcgcc agatggctgg
    31741 gggtacgcca ccatttccac aggcgcttaa tcagaccagg cttacggtca gaatttccca
    31801 taataacctc ttatttcccg taaccttttg atggggtaaa ggtattcccc acgattggcg
    31861 cggtatcggc ctgcggtacg tgacattgca gacagaaata acgacgcgga gccacttccg
    31921 cgcccacttt gccgtcgctg tccataaagt gagtaggact gatacgcggc gcgccagtgg
    31981 tgcgatagct ttcgacaccg tggcactgca agcagcgatt ggtattggtc gttacctgat
    32041 aaccttcaac gctatgcggg atcattggcg gctgattcac atagttcagc ggcatccgat
    32101 cctgctcttt tggcatccga atggcccctt cctgcgtccc ggatacttcc ggcgactggc
    32161 taaaatcgac gccattagcc gcccaaaccg cgccgcttac caccagggcc agcatcgccg
    32221 tccattgaca cagcgctttc ttcaggtcat ggcttttcat gatttcgctc ccgaactcca
    32281 tcgtgtagtt attgtaaata catcctcaga acagacatcc acgcagcgac cgcacgtcat
    32341 gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc agcaccgggg cacgtagcac
    32401 atgcggttcc gggcaaacat gaaaacaatc catacagcgg ttacatttct gccgatcgct
    32461 ggccgcaacg gtaatcacac ctttgctacc cagcacgcca tacagcgcgc caaccggaca
    32521 aatgtgcccg caccagccgt gttcaacgac cagtagatca aataaaaaca gcgcgagaat
    32581 aagcagcgcg ccgctgccga agcccattac caggctacgc cccatcagag aaacggggtt
    32641 gatccattcc caaatgagcg tgccagttaa ggctgatccc accagaatga ccaccaacag
    32701 cacgtagcga atgtggcgag ggatcgtcgc agactgattg aggtcaaagc gcctgcgtaa
    32761 ccagttcgct aagtcggtaa tcgggttcag cgggcagacc cagctgcaaa ataatcgctt
    32821 tcccgccagg gcgtagagca cggtgataat caccgcaccg gtcaacgcca ccgtggcagg
    32881 cagatgacca ctggcaagac tttgcagcgt catcagcgga tcggtcagcg gaacggtgtc
    32941 gaataacagg ctactgctgt agttgccgtg caagatccac acaccaaacc atggaccgct
    33001 caaaaacatc cccagcacga agaactgaca aaggcgacgt aacaccagcc aacggtgact
    33061 gcgccaccag ccttttttct ccagcgcctc gcgcccggcg tcacgtttac gatttgccat
    33121 tgttcccctc cagccagccg aagcggtaat ggtgacctaa ctcccctttc gccagtgaca
    33181 gcggtaacac cttgattgcc ggttgttcca gcacgcatac tttttcgcac ttaccgcaac
    33241 cggtacaggc gtcgctgtga accgtcggca gaaagcgggc gtgcttaccg gtacgcgtgt
    33301 tgcgctccag ctccaaggtg atggcctcat caattttcgg gcattcgcga taacaaacat
    33361 cgcagcgcag cccctgaaag ttgagacagt tttcctggtc caccagtacc gccagcccca
    33421 tccgcgcgtc gtcgatcgat tcaatctcac gatccagcgc accgcttggg cacactttgg
    33481 cgcacggaat gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc
    33541 ccgccgacag accagaggcc agcgtcgcca gttttaaggt gtcgtaaggg caagcctgaa
    33601 cacactgacc gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg gctccgggcg
    33661 ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg taaccccagc gccacaccca
    33721 cggcagccag cccgcctgct gtgcgaacga catcgcgcag aaagcggcgg cgaccatttt
    33781 gaggtttcgc tgaccgggac ataatggctt acaccttctc cagtttgacc gcgcacttct
    33841 tgaagtccgt ctctttcgag agcggatcgg tcgcatccag cgtcagtttg ttaaccagct
    33901 gtgcggcgtc gaagaacggc atgtacacca ggccctgtgg cggacggtta cgaccgcgcg
    33961 tttcaacaat cgagatcact tcgccacggc gagaaaccac tttcactttg tcgccacggc
    34021 gcagatcgcg cgctttcgca tccagcgggt gaataaacaa gaccgcttcc gggaaggcgc
    34081 ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg ccagtgctcc agaacgcgtc
    34141 cggtagagag ccacaggtcg tactcttcat ccggtgcttc cgccgccggt tcgaacggca
    34201 gtgcgaagat caccgctttg ccatccggtt taccgtagaa cttatagcct tcgcccgctt
    34261 tcacgtacgg gtcgttacct tcgctgtaac gccactgtgt ttctttacca ttcaccaccg
    34321 gccagcgcag accgcgcgct ttgtggtagt catcgaacgg tgccagatcg tgaccgtgac
    34381 cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg cagatagaag cccagctcgc
    34441 gggattcatc gttcagctga tcttccgcca gttcggatac cgggaatttg ctcacttcgg
    34501 gggtggcata cagaacttcg tacagcgttt tgccacgcag ttccggtttc ttcgccagca
    34561 gctcttccgg ccatacttct tcagttttga agcggcggga gaactggact aactgccaga
    34621 gatccgattt cgcttcgcct ggcgcctgta cctgctgacg ccagaactga gtacggcgtt
    34681 cggcgttacc gtaagcgccc tctttctcta cccacattgc ggtcggcagg atcaagtcgg
    34741 cggccagcgc actgactgtc ggatacggat cggagacgat gatgaagttg cgcggatcgc
    34801 gccagcccgg catacgctct tcattgatgt tcggcccggc ctgcatgttg ttggtacaca
    34861 tggtccagta aacattcagc ttgccgtctt tcagcgcacg gtcttgtgcc accgcatgaa
    34921 gaccgatttt cgccggaatg gtgccgctcg ggatattcca cttcttctca cagatatcac
    34981 gatgtttctc gttagttacc accatgtccg caggcagacg gtgagcaaag gtgcccactt
    35041 cacgcgcagt accacacgca gaaggttgcc cggtcaggga gaacggaccg caacccggct
    35101 gggaaatttt gccggtcagc aggtgcaggt tgtagaccag gttgttagcc cacacaccac
    35161 gggtatgctg gttgaagccc atcgtccagt aggagatgac tttcttgttc ggatcggcat
    35221 acagctgcgc cagttgttct aactggtctt tcggcacgcc ggtcatttca gcggtttttt
    35281 ccagcgtata ctcagcaacg aaggctttgt aatcttcaaa gctcatcggt tcggaggcgt
    35341 cagaacccgg attcttcgct gctttttcca gcggatgggt cggacgtaaa ccgtagccga
    35401 tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa gaagtcctga tttatcgcat
    35461 tgttttgaat gatatagttg gcgatgtagt tcaggatcac caggtcagat tgcggcgtaa
    35521 agatgatgcc gttatccgcc agctcgaagc tacgatgctg gtaggtagaa agcaccgcca
    35581 cggtgacgtt ctggttagag agacgactgt tagtgatgcg tgaccagagg atcgggtgca
    35641 tctccgccat gttagcgccc cacagcacaa acgcgtcagc ctgctcgatg tcgtcatagc
    35701 agcccatcgg ctcatccata ccaaaggtac gcataaagcc aactactgcc gacgccatac
    35761 agtgacgtgc gttcgggtcg atgttgttcg aacggaagcc cgctttgaac agcttggacg
    35821 cggcataacc ttcccagata gtccactgac cagaaccgaa cataccgatc gattccggcc
    35881 ctttttcttt cagggcggtt ttgaatttct cttccatcac atcgaaggcc tgatcccagg
    35941 tgattggggt aaattcgcct tctttgtcat atttaccgtt tttcatacgc agcagcggct
    36001 gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa atagccctta atgcagttca
    36061 ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc caccacacgc ccctgctgcg
    36121 ttccgaccag aacgccgcaa ccagtaccgc agaaacggca cggcgcttta tcccatttaa
    36181 tggcttcctg ctgaccaaca acggcgcggg caacgcccgg cacgctgaga ccggcagccg
    36241 ccgcagcggc cgcaacggcg ttagctttca taaagctacg acgactgagt ttcatggtgt
    36301 ttcctcacct tgctcttcct gctggtgata aaccagcgac accgccagca cgccctctac
    36361 gttgcgtact gactcaatgg tttggatcag cgtttcgctg tcttctgctt ccaccaccac
    36421 aatcaactga ccgctcggcg cgtcgctgac agcaacttca cagccgggaa aggcgttcag
    36481 ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg
    36541 ccagttagtg tgcatggaga tactccgcag ttatggctga taccggacag ctggctgcac
    36601 atgctccaca tccgttgcaa agttggctat taagttgcgc ctggtagatc ccggagagcg
    36661 tcgggcgaaa gataattgcc attggctcac agctatcctg acagcggcgg cattcaactg
    36721 actgatacgc caggcacgcg tccccgatgg tgaactgcaa atcccaggcc ctggtgtggc
    36781 gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt
    36841 tatttttgaa attaacgctc ggatagccac ccgtgccgcg ttgcagaatg ttgttttcac
    36901 aagcattaat acaggcgtcg cagcgggtgc aatgggtcag aaaatgagat tcatcaccgg
    36961 accagggcgg acggataccg ttactggctt tgcgccagca accagtgagt atgccccgac
    37021 gggatgcatc aatcttcaca ttgaccttcc atcattaacg cgctctgaaa attgagagcg
    37081 accaaataga ccgcataatt aataagccat ttttatagcc gctaagatat taaaggatgt
    37141 gtcaaagatg cataccccga tcggggtaaa acctgtagca ggatcaaaag agtggcggga
    37201 agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt tataaacata tttatatagc
    37261 gattaattca ccagagatat ttctgctggt ttgctccctc atcagaattt aacactaaaa
    37321 gagcaggtaa aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg
    37381 atgaatatgt taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc
    37441 aaaaaaatga agaccattct acctgaagta ttgtttgccg ctttcgctac cacttccgcc
    37501 tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaca
    37561 ggcatgaagc gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta
    37621 gaactgttaa tcggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag
    37681 ctggaaagca aaacgctgga aggctggggc tatgattatt atgtctttga taaagttagc
    37741 tcgccggttt ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc
    37801 gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat
    37861 acgccagaca atgtagatgt gaaataccgc gtctggaagg cggaagagaa aattgacaac
    37921 gcggaagttc gctaaactgt tgtgaagtac ggcaccccgt tggttggata aggcggttac
    37981 gccgcatcca acatctaacg cccgagccgg ttgcctgatg cgacgctggc gcgtcttatc
    38041 agtatacgcc acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt
    38101 tcaactgcgg tttcggcgta ctatctgctg cttgcggttt gtcgtatttc agccccagca
    38161 cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc
    38221 cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc
    38281 caaatacttt ttccagtaga ttcaacataa tcggcgcggc ggttgacgcc cctggcgatg
    38341 cccccagcag cgccgcaatg gtcccttgct ggtcactgac cacttcggta cccagacgca
    38401 gtacgccacc tttctcggca tcacgcttga taatctgcac acgctgcccc gcttgccaca
    38461 aacgccagtc ctcttttttc gcttgcggat agtactcttt caacgcttca aaacgatcct
    38521 cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccga
    38581 cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc cacaatgaac
    38641 cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac
    38701 cgtccagaac acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt
    38761 taccgtaaac tttcgccaga tggcgattaa ccacgtctgg attttccgaa acaaggaact
    38821 gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat tcctgtaaca
    38881 gcttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcgcggatg ttctgtgcag
    38941 tgccgttttt cagatcggca acggtaacgg tccaggtgtt atcgtcatta cgctttaggg
    39001 cgcggacttc gctgctgagt tgcagcgaga agttagattt cttctgtaag gaagcaatta
    39061 actggcgggt gatctcacca tagttcacat cagtgccaat ttctgttgcg gatcgcgccc
    39121 ttccatcact aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc
    39181 gcgaaacagc gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat taacgttatc
    39241 ctcgccccag acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag
    39301 cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg cttcgttaat
    39361 ggcgactgct ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt agttcagttc
    39421 catcagtgca gaatgcccgg ttccggcgtt gttctagccg ttcgaactct cctgcgcgcc
    39481 cccctccagg cgctccacca tggtcatcga ccattcaggc tccagctcgc gtcaataggt
    39541 ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc
    39601 ggacgctttc gcttttgccg ccatcgaaac ggcattaagc cccacggcca tcgagaagag
    39661 catggcagtc acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg
    39721 agatttgcgc ggcatcaacg gtaacactta agtaacaaca tttaaataat gtttataaat
    39781 tatattctaa ttttagaaac attataattt cgttgattaa ttatagggtt attagaaatg
    39841 aagggatttt tagcatattg acctgacggc agcagtccgc caggtcagcg tcgtaatatt
    39901 atgccgtccg ggcaacggca tcacgcgaag cggcatcgcg ctcttcgccc gtcagctcgc
    39961 taagttgccc attgcgcatt tccagcaggc ggtcggcgtg gataaagtaa tgatcatcat
    40021 gactgatagc gaaaatagtt ttacccatct cctgcatcag cggcagcaac acctgataaa
    40081 actcacgacg gaagtgtgga tcctgatcca ccgcccattc atccagcagg ataatatcgc
    40141 gttcttctgc cagcgccagc aacagcgcca cgcgtttttt ctgccctttt gataacttca
    40201 ggttaacaat acgcccgttg cttaactcaa gcttatgagc cattttcagc tgcgccagcc
    40261 acttctcaac cagttgcggg ttagcgggtt gcccctccgg ccccagcagt tgatcaaaca
    40321 gccagacatc ggtaaacact gccgaaaaca gtttgcgata atcttccggt tgttcgccgc
    40381 tgacaggttt gccatccagc aagatttcgc cgctttgtgg ctgatacaag cccgtcaaca
    40441 acatcgccag cgtcgatttt ccgctaccgt taccgccaat cagaaacagc agctcgccac
    40501 gtttgatggt gagattaatc ggaccaacgg aaaacgcgtt atcctgataa gcaaacgtca
    40561 cgttacgcag ctccagcgtt tgccagttgg gaaacgcctg cgggcgcgga aactcagctt
    40621 tgaaaggcgc gagcgcgaat ttgttcagct tgttaaacgc cacctgcgcc gtcagcagcg
    40681 tcggcaatgc gccaaccgcc gaaagcagcg gcgtacgcag gaataacagc gtcaacgaat
    40741 aggtcgcagc aacgttggta tcagcccaac cgagactgtt cgccatccag aacaccaggc
    40801 cgatagcgcc cagcatcatg atgtttgacc agttcacggc actaagatgg aaggtgtctg
    40861 cgcgaataat atgatggcga tactcttgcg catcggggat gtagaggttg ttaaacacat
    40921 actcggcgcg ttcccggttc agagtcagct ctttgcgccc ttcaagtaca gtttgaaaat
    40981 ccgtgtacag cttgtcttcg gtttcacgta gggtcgccat atgtttgtac acccgcgcca
    41041 ccagcacaaa accgccccag atggtgatcg ccatccagat agccgttacc agcaacattt
    41101 tgcccgacag catccacaga tacgccgatg aaccgatagt gagaatgatc ccctgcccca
    41161 gttccggcag acgcacaaaa gcaatggtga tattgcgcac atcgctggtt aatcccgcca
    41221 gcaacgaggc gctaccgagt tgttcaatgc gctcgacgtg ggtatccaga atccgcttga
    41281 taaattcact acgcagtcgg taaacgaagt gatgccccaa agtggtgagc gccagttgcg
    41341 atccgagagt gactgccatc aacagcagca ataatcccag aaactccggt aacaccagca
    41401 gactggtatc cgccgtttcg ataaggcgct gattgataaa agcaattaag ccaatgccta
    41461 atgccgcact ggcgaggctt agcgccatca cactgataaa tggccagcga tactgccgcc
    41521 agacaagtac aagaagttcc atgcagaaaa cccggacaat gaattacagc ccgcagttta
    41581 aacatcttcg cgcgcacagc aataataatt cttattttta ttctttttta cctgcctgac
    41641 ggaatgtcag gttgtagcgg cagtcggtgg tgagtggatg aaaccccgct ttcaacggtt
    41701 gaataccgtg ataaaacagc cgcgattcac cgccccatac caccacatcg ccatgttcca
    41761 acaacaaacg tttgagcgga tcattacgtt tcaggccacc aaattgaaaa atcgcgggta
    41821 agcccagaga aacagaaaca attggcgcgc gcagatccgg ttcgtcttta tcctgatgca
    41881 gcgacagttt cgcgccagga gcgtagcggt tgataagaca agcatctggc tggaaatctg
    41941 gatagcccgc cgccgtagcc gcacgttgac ataaatcatg aaagctttgt ggcatagcgg
    42001 gccacggttt atttgtttgc ggatcaatgg gcgaatagag ataaccttgc cgatgggtcg
    42061 tccagcccag atgcccacag ttggtcatcg ccaccgacat ggtatatccg cctggagcga
    42121 ccatctggcg aaacggcgac tggctggcaa cgtcattaat atcgcggatt agttgctccg
    42181 cagcattaaa agcaaaacgc cgtaaaatta ccgcaccagc cgccagtggc tcttgccacg
    42241 gttcagcatc ggcaaacaga tccaacatta cctctcctca ttttcagctt cgcggcgcag
    42301 cagttgcgct ttacgcgaca cgccccagcg gtaaccggaa agtgtgccat caccacggac
    42361 cacccgatga cagggtatta cgatagccag cttgttggcg gcacaggcgc tggcaacggc
    42421 ccgtaccgct ttcggtttgc cgatggcgtt agccagttgc tgataactga cggtttcacc
    42481 acaaggtatc gtgcgcagtg cctgccagac ttgttgctga aaagcagtgc cgcgaatatc
    42541 cagcggtaac gtcagcggcg tatcgcgttg attgaggctg gcgatcactt cacgcacatg
    42601 ttgctgaaac gtcagatcgg caggcgcgtt gtcggcagcg ggaaacatct gctgcaactc
    42661 gctgattaat gtcgcgtcat catcgcccag caatatcgcg caaatccccc gctcgctttc
    42721 tgccaccagg caacgaccca gctcacaatc agccagcgcg taacgcaccg ccagattttc
    42781 gccaccgtga cggaattgtt tagccgtcat gcccagcgtt tcatcggctt tgcggtagta
    42841 actgctgctg tcggggaatc cggcgttaag aatagacgtc gtcacgctct cccctttcgc
    42901 cagcgattcg cgcaaacggc gagcgcgcca ggcctgttgc caggctttag gcgtcattcc
    42961 ggtagtagct ttaaacaacc gatgtagatg aaatggactc atcgccacct ggtcggctaa
    43021 ggcttccagc gttacaggcg tttcctgttc cagcagtcga cacgcgtggg tgattttatc
    43081 caaccgatgt tgccggggat tggctttgtc tggctgacaa cgtttgcagg ggcgaaatcc
    43141 ggcggcgagt gcctcgctgg catttgcgta gaaggagaca ttttcccgca aggcatgtct
    43201 ggcgcggcaa gaaggacggc aaaagatacc cgtggtgcgc acggcgaaaa cgaattcgcc
    43261 gtcggcattc gggtcgcggg ctaagacaga ttgccagcgt tgatcgtcag ttaagcatgt
    43321 ggcgtttttc ataatcagct ccctggttaa ggatagcctt tagtctgccc ggtcaccatc
    43381 acgcaaaaac caacaatctt gcgctttaat ttttttcgct gacaaggaag cttttaaact
    43441 gtggtgacat ccaggtttta aagctatcgc cttctttggt gatcatataa accgccagcc
    43501 cttcccggcg aacaacctct ttggctttct ccggcccgag taccatcaag ccagtatccc
    43561 aggcatcggc ttccagcgcc gtcggagcaa tcaccgtcac ggataccaga ttgtgttcga
    43621 tgggacgccc ggtttgcgga tcgataacat gggaaagacg tttgccgtcc agttcgtaat
    43681 agttacggta gctgccagag gtgctaatcc catgaccgtt gatatccacc acagcctgaa
    43741 ccgcgttttc tttatcggtt ggtttttgaa tcgctacccg ccacggctgg ccttcaccgt
    43801 tcataccacg gctgttcagc gcgccgccca ccgacaccag atagcgggaa atcccttcct
    43861 gctccatcaa gcgtgccagg tgatccgccg cataaccttc accgacggtg gagagatcga
    43921 catataaatc cggcaggtct ttttgcagat attgctgatg cgactgattg atgaccgtca
    43981 ggtgctgtaa gccggttttg gctttcatcg catcgatctg ttcctggctc ggaatttgaa
    44041 ccggctgttg ttccgggcca aagccccaca gattcaccag cggcccgacg gtaatatcca
    44101 tcgcaccatc ggtccttgcc ccaatgcgca gcgaggtggt gacgatatcg gccatcgctt
    44161 cacttaccgg ccacggcgac aaactttgcg agtcgttaaa gcgcatcagc gcggaatctt
    44221 ttttataggt cgaaagcagc tgatcgtcgg cgtccagctg ggtctgaatc ttttctttaa
    44281 gttcggcact gcgtttggca tcaatgcccg ggatgctggc acgccagaag gtacccatgg
    44341 ttttgccttc aagaacggta acttcagtag cgtgggtttt ggcgggttgt ggtttttgat
    44401 cgcaaccaac aaagaagagc gcggcaacca gcagtgccac gcgggtaaag cttatttcca
    44461 tacgtgatta tcctcatgcg aacggtcgca agagtacacc aaaaaactgt gtttgtacgc
    44521 tgaaaacaat gaaaaaaggg cccgcaggcc ctttgttcga tatccatcga gattagaact
    44581 ggtaaaccag acccagagct acgatgttat cagtgttgat gccagcgtca cgagtgaact
    44641 ggttgtcgtc cagcaggttg attttgtagt caacgtaggt ggacatgttt ttgttgaagt
    44701 agtaggtcgc accaacatca acatatttca ggatatcttc gtcgtcgtag ttacgaccat
    44761 tgatgacacc caggttttta cctttagact gcaggtaagc cagggacgga cgcagaccga
    44821 agtcgaactg gtactgagca acagcttcga agttctgtgc tttgttcgcc caacccaagt
    44881 tgcctacgcg agttgcgttg taggtctggg tgtactgagc agctaggtag atgttgttcg
    44941 cgtcgtattt cagaccaccg gtgtaggttt cagcgcggtc gccattaccg atgtaacgct
    45001 catcataacg gttcagaccg aagttctggt catcagtacg tttggagctg gaaactgcag
    45061 caccgatacc gaagccttcg taatcataag taatagaacc gccaacgccg tcaccgttct
    45121 gacgcagagc ttcacgaccg ttgttggtca tgccttcacc ttccgggcta ccgtttttgc
    45181 cctgatactg aacagcgaag ttcagaccgt caaccagacc aaagaagtcg gtgctacggt
    45241 aggttgcgaa gccgttacca cgctgctgca tgaagttgtc agaaccgtaa gtgtcaccac
    45301 cgaattctgg cagtacgtcg gtccaggaag ttacgtcgta aactacgccg tagttacgac
    45361 cgtagtcgaa agaacctaca tcctggaatt tcagacctgc gaatgccaca cgggtccagg
    45421 agttgttttc gttttcagcg ctgttgccct ggatctgata ttcccactgg ccgtaaccgg
    45481 tcagctggtc agtaacctga gtttcacctt tgaagccaag acgcatgtag gtctggtcgc
    45541 catctacaga cttgtcgtca gagaaatagt gcaggccgtc tactttaccg tacagatcta
    45601 atttgttgcc gtctttgttg taaacttcag cagcgtttgc tgcgcctgct accagcagag
    45661 ctgggaccag gagggacagt actttaactt tcatgttatt aaccctctgt tatatgcctt
    45721 tatttgcttt tttatgccac tgcatactga ttaaccctca ttaatcagtc ggcaagtcca
    45781 ttctccccaa aaatgcagaa taatccaaca cgaatatgat actaaaactt ttaagatgtt
    45841 tcatttatcg ctatagatgt ttcaaaatgt aaatacaagg gaacttttta agattattgc
    45901 ggaatggcga aataagcacc taacatcaag caataataat tcaaggttaa aatcaataac
    45961 ttattcttaa gtatttgaca gcactgaatg tcaaaacaaa accttcactc gcaactagaa
    46021 taactcccgc tatcatcatt aactttattt attaccgtca ttcatttctg aatgtctgtt
    46081 tacccctatt tcaaccggat gcctcgcatt cggttttttt acccttcttt acacactttt
    46141 cattattctg tgctaccaca gaaaaactat aacgcttgtt aactatttca caaataatta
    46201 acatcagcat aatttccagc aatctttgtt tatttgcaat taatttcgtt gggctttttg
    46261 taggttattt gtacagcaaa atggcgcttg tacatctatt tccccccata caggatgata
    46321 aatatcacgg gagaatagag aatcatcaat caggtaagag tctggaattt cacactgtac
    46381 cctttatact gccctatcac ttcgcgaagt tttaacaggt cataaacacg aatgcgtcag
    46441 aaagagacaa cggccacgac ccgcttttca ctcctaccgg ggagcattac ccgcttcttt
    46501 ttactgttga tcattgtgtt actggtgacg atgggggtaa tggtacaaag tgccgttaac
    46561 gcttggctga aagataaaag ttaccagatt gtcgacatta cccacgcaat ccaaaagcgc
    46621 gtcgatacct ggcgttacgt gaccaggcag atctacgaca acattgccgc gacgacctcc
    46681 ccctcctccg gcgaaggttt acaagagacg cgcctgaaac aggatgtcta ctatctggag
    46741 aaaccacgcc gcaaaacgga agcgttaatc tttggctctc acgacaactc aacgcttgag
    46801 atgactcagc ggatgtccac ttatctggat acattgtggg gtgcagaaaa tgtaccgtgg
    46861 tcgatgtatt acctgaatgg tcaggataac agtctggtgc tgatctcaac cctacccctt
    46921 aaagatctca cctccggatt taaagaatcg accgtcagtg acattgttga ttcacgtcgt
    46981 gcagaaatgt tacaacaggc caacgccctc gatgaacgcg aaagtttttc taacatgcgc
    47041 cgcctagcct ggcagaacgg tcattacttt accttgcgta ccacgttcaa ccagccggga
    47101 catctggcaa cggtcgtggc ttttgatctg ccgattaatg atttgatccc accgggtatg
    47161 ccgctggaca gtttccgcct tgagccagac gcgacggcaa cgggaaacaa tgataatgag
    47221 aaagaaggga cggatagcgt cagtatccac tttaacagta cgaagattga aatctcctcg
    47281 gcactcaact ctaccgatat gcgcctggtc tggcaggttc cttatggcac cttattgctg
    47341 gatacgttgc aaaacattct gctgccactg ctgctgaaca tcggtttgct ggcgctggcg
    47401 ttatttggct ataccacatt ccgccatttc tccagccgca gtacagaaag cgtccccagc
    47461 acggcggtca ataacgaatt gcgcatttta cgggcaatca atgaagagat agtctcactg
    47521 ttgccgctcg gcctgctggt tcacgatcag gaatcgaacc gcactgtcat aagtaacaaa
    47581 attgccgatc atttgctgcc gcatctgaat ctgcaaaaca tcaccaccat ggcggaacag
    47641 catcagggga ttattcaggc gacgatcaat aacgagctgt atgagatccg catgttccgc
    47701 agccaggtcg cgccgcgcac acaaattttc attattcgcg atcaggatcg cgaagtgctg
    47761 gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga aaaaccagca ggggcggatg
    47821 acctttatga aaaacattgg cgatgcgctg aaagaacccg cacagtccct ggcggagagt
    47881 gcggctaaac tcaacgcccc ggaaagcaaa caactggcga atcaggcaga tgtgctggtg
    47941 cggctggtcg aagaaataca gttagcgaac atgcttgcgg atgatagctg gaaaagtgag
    48001 acggtgctgt tctccgtgca ggatttaatt gatgaagttg tgccttcagt gttgcctgcc
    48061 atcaagcgta aaggtctgca actgctgatt aacaatcatc tgaaagcaca cgatatgcgc
    48121 cgcggcgatc gcgatgcctt acgacgtatt ttgctgctac tgatgcaata tgccgtgacc
    48181 tcaacgcaat tgggaaaaat cacccttgag gttgatcagg atgagtcctc tgaagaccgc
    48241 ctgacattcc gcattctgga cacgggagaa ggcgtaagta ttcatgaaat ggataatttg
    48301 cacttcccgt ttatcaacca gacccaaaac gatcgttatg gaaaggcgga cccgctggca
    48361 ttctggctga gcgatcaact ggcacgtaaa ctgggcggtc atttaaacat caaaacgcgg
    48421 gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc tcgccgctga cccggaagtt
    48481 gaagaggaag aagagcgttt actggatgat gtctgcgtaa tggtggatgt tacttcggca
    48541 gaaattcgga atattgtcac tcgcctgtta gaaaattggg gtgcaacctg tatcacaccc
    48601 gatgaaagat taattagtca agattatgat atctttttaa cggataatcc gtctaatctt
    48661 actgcctctg gcttgctttt aagcgatgat gagtctggcg tacgagaaat tgggcctggt
    48721 caattgtgcg tcaacttcaa tatgagcaac gctatgcagg aagcggtctt acaattaatt
    48781 gaagtgcaac tggcacagga agaggtgaca gaatcgcctc tgggcggaga tgaaaatgcg
    48841 caactccatg ccagcggcta ttatgcgctc tttgtagaca cagtaccgga tgatgttaag
    48901 aggctgtata ctgaagcagc aaccagtgac tttgctgcgt tagcccaaac ggctcatcgt
    48961 cttaaaggcg tatttgccat gctaaatctg gtacccggca agcagttatg tgaaacgctg
    49021 gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa aatacatcag cgacattgac
    49081 agttatgtca agagcttgct gtagcaaggt agcctattac atgaacaata tgaacgtaat
    49141 tattgccgat gaccatccga tagtcttgtt cggtattcgc aaatcacttg agcaaattga
    49201 gtgggtgaat gttgtcggcg aatttgaaga ctctacagca ctgatcaaca acctgccgaa
    49261 actggatgcg catgtgttga ttaccgatct ctccatgcct ggcgataagt acggcgatgg
    49321 cattacctta atcaagtaca tcaagcgcca tttcccaagc ctgtcgatca ttgttctgac
    49381 tatgaacaac aacccggcga ttcttagtgc ggtattggat ctggatatcg aagggatcgt
    49441 gctgaaacaa ggtgcaccga ccgatctgcc gaaagctctc gccgcgctgc agaaagggaa
    49501 gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa atcagtgctg gtggttacgg
    49561 tgacaagcgt ctctcgccaa aagagagtga agttctgcgc ctgtttgcgg aaggcttcct
    49621 agtgaccgag atcgctaaaa agctgaaccg cagtattaaa accatcagta gccagaagaa
    49681 atctgcgatg atgaagctgg gcgtcgagaa cgatatcgcc ctgctgaatt atctctcttc
    49741 tgtgacctta agtccggcag ataaagacta atcatctgta ggccagataa gacgcgttag
    49801 tgtcttatct ggcatttgca ccgattgccg gatgcggcgt aaacgcctta tccggcctac
    49861 gattcccatt atttcaacag attacattaa agtaggccag ataagacgcg tcagtgtctt
    49921 atctggcatt tgcactgaat gccggatgcg gcgtaaacgc cttatccgtc ctacgaatcc
    49981 cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct gttttatcac atccagcgtt
    50041 accggcttcg agaggcagct gtccataccg gactccagac accgctgctt ctcttcagcc
    50101 aacgcattag cagttactcc gattaccggc aacgtcagtc ccaactgacg aatgcgttgc
    50161 gtcaagcggt aaccatccat atttggcatg ttgacgtcgc taagcacgat atcaatatga
    50221 ttcttactaa gtacattaag cgcatcgacg ccatcattcg cggttttaca ttgatagccc
    50281 aacgatccca actgatctgc cagcagacgt cggttaatcg gatgatcatc cacgaccaga
    50341 atcatcatat cgtcgttatc gctgaccgct ttgtccgtcg acggcagagc gttagcagga
    50401 tcgtcgctct ccatctcgat caaataaata cgcgccaaca atgccggtag ctcatgcgga
    50461 gcagccacac tgtgtaccca ctcccctggc gctttctcca gcggaatacc aatatggcga
    50521 cgacagaagg tcactaccgc tctgccctgc cattttttac tcactacctc gtcagtgatt
    50581 aacacatctt cgggagtcgg ttcctgccct tcgtatgttg taacgacgat gccgctgcgc
    50641 tgcaaactgg tttccaggaa ctgacagagc gacgcattgc ggaccgccag ccagcagcgt
    50701 ttaccactca acccttccac gccttttttc tgcgggtact gagcgccgta caacggaata
    50761 cgcacggtaa actggctgcc cattcccggt tctgaatcta ccgagatatc gccgtccatc
    50821 atgctgatca gtttttcaca aatcgccaga cccagaccgg tcccctggaa gtttcgctgt
    50881 acgcccgttc caacctggaa gaagggatca aacaagcgca ccacttcttt cgccggaatc
    50941 cccacgccgg tatcgcgaac acggatagag agataatcgc catcagcgcg aacatgcaaa
    51001 actatacagc cggtatcggt gaattttatg gcgttactca acaggttgga gatgacctgc
    51061 tgtaaacgca tcgggtcgcc atttaaggcc actggcacat ccggttcaat aaagcagtac
    51121 aagcctaact gcttgcgtac caccagcggt aaatagttgg cggtgatgtg gttcatcact
    51181 tcacgcggtg aaaactcacg cggttcgatc ttcaactgtt ccgattcaat cttcgagaaa
    51241 tcgagaatat cgctgataat tttcaacaac aggctggaag agttgttcat tgccgtcacc
    51301 agacgatcga cgcctttcgg taactctttg gtttgcaaca gatccaggtt accgataatg
    51361 ccatacagcg gcgttcgcag ctcatgactg acggtggcaa ggaacatcga ttttgactgg
    51421 ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact cttccatctt cacgcgcgaa
    51481 gaaacatcca ccagcacaca aatggccacg ttttcattac gatagcgcga atggacgaag
    51541 ctgatttgca gattggtatt gttgctggtc aggacatcaa caaaattgac ctgctgccca
    51601 cagataattt gcgtcagtcg ttggcggtcc tcatgcgtaa gcatattgag ataggtatgc
    51661 gccagttcgt tacttaaaat attgacgcca tcagcggtac gcaaaatgca gatacccact
    51721 ggcgcggagg cgacaatctt gcgattgaac tgctcatgtt cttccagtcg cagggcgtcg
    51781 ctttccgccg gaatgaaaat acgtcgctcg tacatccgcg cgagagtaaa caatgcagct
    51841 ccggcaagca cattcagcaa aattgcgtta aggatcaaca tgcgaatgcg ttccagcacc
    51901 ttatcaaccg gcaccgaata cacgatgctt agcgatgagg gtggcagatt tttcttcagc
    51961 accagctccc ggaacccttc cgtatagcca aaccaggagc gttcctgcat ccagcgagga
    52021 tcgcccttaa ttttactttc tggtccggta agcgaaatca gggtatgacc attttcatca
    52081 agaatggtaa cccccatcgg caacgtaccc ggtaagaaaa agttctccat ccggatggtc
    52141 tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat aaactggcgt caacgcgtaa
    52201 aaatacccga cgcaagggcg cggaccttcg ctgatccagt agaggttact gccgctatca
    52261 tcttgtggtg catttcgata tttattgatg cgttcatgca acgctttcaa cgcggtatcg
    52321 cgttccactg gcatatcacg cagaccgaaa ttggccatgc agaggttatc actgccgatt
    52381 aaaaataccc ggttgagatc gtaagccgca gaaaaattat cgcgccagta gcgcataaac
    52441 cacgccaatg actccagaga acctcgccag gtgttactca ttgcggaaca atcggagtcg
    52501 gcaaacagcg gttcaaacgc aggcacatcc gcctgcgttt ctcgtccacg cggggaaagc
    52561 acaccgtttt ctgccgataa gcgattttcg gcgatgtact taagctcttt catcacatca
    52621 gaggtgcgtt gaataaagcg ctgagcctga tcggaactca gattaaattc ctgacgaatt
    52681 tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa cggatgaaaa agcaatcaac
    52741 agccagagca ctaacgccaa tgctctgaac atgtagcgcg aggctttcag ggttgtacga
    52801 aaagaagcaa ggtatttcaa aggggagaag ctccgcctca ggtgaccgat ggagtgtggt
    52861 taaggtagcg gtaaaagcgt gttaccgcaa tgttctgtca ccattgcaat aatgcgtatc
    52921 gcttagaaac cattgcctta accaggaaag ccggagtaga tggacagttc tcagtacgaa
    52981 aaataagaaa tgaccaatct gccgacgctt gttaatggtt atatcctggc gtcggcagat
    53041 cgcccagcag tcttatctga aaaaagagag agccaggttg tgtgaaggga tggatcacca
    53101 gtcaaaactt ttcaccagcg tcagctcgcc agcattacgc atcggtacaa taaatgtttc
    53161 ctgtttctca ttgaccgatc cttcatcggt gatcagcgtc agttgggcgg tcgtcaattc
    53221 cgtttcgctg cgcccaccat agtagttgat atacacctga tagcgcccgt gaactggtgc
    53281 tggcatggcg aaaatctcgg gtccgtaccc cgtcgtgaca tccatatcca gtgcaccact
    53341 gtttttcagc acggtgttac cgtaccaggc gtgttcgcca tcgggcgtga caacatggag
    53401 atcgagatcg gtattgtccg tatcccacga gagaaccagc cgtaaacgtg cacgaatagt
    53461 tcctgtaccc ggcgttgagt aaaactgcat tttttgccgg ctttgcccat ccgggctgat
    53521 gacctgcacg ctgttgctgc cttcagtgaa aatataaggg cgtgcaaatg aaccatcgga
    53581 ttcaattctc tgcggcatac tggcaccgtt gaccaccagc cggccctgct gaactttacc
    53641 cgccgcagca taattcttta ttttgccgcg gatctgcgct gagatatttt gatcatctgc
    53701 catgttgacg gacgacgccg ggtaattgat cgattgtgaa aaactggcat cttcgccctc
    53761 tgccggatgc cagccagaga gcggtgcgtc aatctcgact tcctgcacac cttcactgtg
    53821 agcgacaggc gaaagtgcca ccagtaacaa cggaagaaaa atctttcgca tagcattagt
    53881 ccattaataa ttgtcgggtc agcgtttcga tatagttctc atcaagcccg gtgggataac
    53941 catcaaaggc caagtgcaga tactcatggg ttaaatccag ccgatcctga agcgtaaaga
    54001 agttacgaat aaacagccgt ttctgctgcc gatcggtata ggggaaacct gagactaaac
    54061 gacagaccgc aaacacgtct ggttcgttgt accccgtctc gccttgtaat atacgccgcc
    54121 actgcggcat ttttttcgcc agccaggctt tcgctttggg taataactgg caggttgagc
    54181 gtggagctcc ccagcggcta aggctgttgt cgggataagc aaacgcgagt atctggtcgt
    54241 aacgctcccc ctgccccgcc tgggccgtgg cttgccgcca ggaaagtgtc ccttcggtag
    54301 cgcgactgcc atgataatga accggatcgc ctgcatagat gagctcctgc gtccaggcgg
    54361 tcatcgttcg cgcccccgtg gtcgctggcg aagcagaaac acgctgcgtg gcgctgctgt
    54421 cggggatggt caggcaatca ccttcccgat tggcgttttg ctgtaaaaaa gtacgtatcg
    54481 ccacagtcat ggctttcgct gcctcaggtg gcgtactttt cgcctcgcga tccagaactc
    54541 tggcaacgta ctcttcacga tcgagatgcg actgcaattt cagtttgccc ttttcgctca
    54601 gtaaggtcgt ctcaccgtgg ctgacaaacg taatgtggtt accattagta aacgtcacac
    54661 gatagcgccc attaagtacg ctgggtttta ctgccgtcgt actcttttcc gctgtgattt
    54721 tcttcagcgg atagcgggca aacagctcca cctctacaca ttgcccactg gcaacctgcg
    54781 tcggcactgg caaaacccga ttaagcaccg tcgcataacg ggttagtacc gttttgctgg
    54841 tgccactgcc agtaacccac agcggtgtac catcggttaa ccagccagca aaaccaccct
    54901 gacggatttc ctgtttatca tcggcaaacc agctccaggt tttcacccgt acccgactac
    54961 cgagcatgga tgcgacaccg attttcgcct cgtccagcac cacatccagt aagacttcct
    55021 gagctttgtt ttgcgcaggt aacgtcgcta atgaatcaag cagcgattta accgttaccg
    55081 aggtttctgg cttcagcgtt gtaagagacg ctaaccacgc aggtgcctga cgctgttgcc
    55141 agtattgccc ccacacatct gcgccaatat gcaaccgctg cggcgcaaaa tagagcccac
    55201 aggaacgtac cagcgcggta tcgcgcgtta tgctctcccc agcctgacaa caataaacct
    55261 cttccgggga gtttccgcga cactggtagg gctgctccgg ttgatgggta tcctccagcc
    55321 aggcgtaaac atacagtttc cacaaactcc ctaacggcgt ggtgagcgag tcaggtaatg
    55381 ctgagacttt ggtcacgcct gatgacgaca gctgatacag ttgatcgtgc tgcgcaccgc
    55441 gcagcaccag ttgtaacggc gtctcctccg caagcgttgg cagagtaacc aacgccaata
    55501 gccagacgat tctgcgccag ttcacttatt tcacctgcat cccggtccat tcactccctg
    55561 ccgcgacact ttgctgcgca ggtgcatagg aacgcacata acgagcagga ggcagaacga
    55621 attgcccttt ttgcgagaag cgcagcaaat ggcggaaagt gaccgttccc gtcagttctt
    55681 tcaccggcac catataggcc agttcgccca tttcattacg cgctttttcc agcaattgcc
    55741 cctgctgttt cgcggcgttg ggtttattga ctgaaatgcc ccatgttgtg cgctcaacgt
    55801 cagctcccgg cgggagcggt acttccacct gaccatagcg cagaactgca tcctgctcgc
    55861 tggtaagcgt aatttcatcg agatacagcg catcgctgtc aatctcattg ctggtcaccg
    55921 gctgcagagt aaagctcatc tcttcttcac cggggataag ccgatacaac tggcgttcaa
    55981 cggtcaccgg aatgttactt tgttgagccg tttttgccgg ttcacgccag cggacctgca
    56041 catttgcggc gataattcgt caccaaaaga gagaatgtcc ggcacaccct aaccaaccca
    56101 acgccagtct tcgccccctc cagttaactt atgtttagcc catgcgcccg caggcgcagg
    56161 caaaacaact ggaggcattg tcgccatata tttcgccagc cagttcatgg ccagcgcgcg
    56221 ctcaatagtg gattgctcag cggtcaaacc attaaaatag cggccgtctg ggtagcgtca
    56281 ccaccgctat tgagcagcac aaccgtgtgc gccagcggct gattgctgtt tgccgcttta
    56341 tcccacgcat attgctgaac ctgtggcatc accgtggact tcaactgcgc ttttttcagc
    56401 aacgtcatgg ttaagacgtt tgccaccgca tctgccagtg gagattccgg tgtatcgagg
    56461 atcaggctgt cattgatatc gcggatgtct tcctcagaga aatcttcatc tttagttccg
    56521 cgccgggcga tagcttcatc cccccctttc aacaacgttt tgcacggcag attcatctcc
    56581 tgtgcccacg ccagcaccag cgcccgatgc aataacggca tattatccgc ctgctcggca
    56641 taactgtcga gcatatgctg ccagtattcc ggttgttgcg ttacgccgag cgcctggctg
    56701 gcctgccagt cggcgtacca tgcccatgcc gtaaggaagg cgtcaccatt gccatcttca
    56761 ccccaccagg taaagcgcgc tccgggcccc gccagttgca tcagccgcag acggttatcc
    56821 tgaatcatct gacgaatgtc gttagcggcg gcactttgat gatcggcaag cgaacgccag
    56881 gcgagactga gcgggatcag acggctaccg gtgttgatca ccccacccca cggttcatcg
    56941 actaacgcat caagattgtt gcggaaaatc tcctgcggcg tttcactact ttgtagccgg
    57001 atattactcg cctgctcggg caacatcagc gcgttctcgc caccgccgag catgacattt
    57061 ttctgctgtt caacgggcca gctgttgtcc acaaaagaca gttttgtgct aatgctgtcc
    57121 tgcacttgcc cattttgttg cagttctgca cttaacaagc cagattgctg aatattctgc
    57181 gtcagcgaaa tataattcgc ccctttgtgc agcgtcagcg tctggcgcat ctcagcgcct
    57241 gcaaatttag tcaccagcgc caccggttcg ttatcctgct gactgaagat aaacagtcct
    57301 gccgccggtt tgtcgcccac gcgatacacc gttggcatac tccacttcat gtagagattt
    57361 ttttccgaac gcagataagc acgcccctgc ccgaccagcc cgtcgccgtt catcccacgc
    57421 gcggtgatac gccagcgggt taacgaatca ggcatcagga acgtgaaata cgctttgcct
    57481 tgtttatcgg ttgtgagtga cggcatccat gccgcggtat ccacctcttc acgccgtgga
    57541 cgttcaagca tttttactcg ccgctcgctg cggttagtcg cgccaggcgc aaccggctcg
    57601 ctggagagcg cctggtcata gctgataaac gacagactgg agctggtacg cacattgtta
    57661 cgccccagcg gatagaaaaa tttgccgata ttcggcgcga tttctggttg cagcgcgtag
    57721 atcatttcat cgaccacgcc taccgttagc tgcgcagaaa caggtttacc tttcagcgac
    57781 gaggtta
//
LOCUS       DAJVPT010000011        52602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVPT010000011 DAJVPT010000000
VERSION     DAJVPT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..359
                     /locus_tag="OQM47_000673"
     CDS             <1..359
                     /locus_tag="OQM47_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1478948.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(397..1296)
                     /gene="nhaR"
                     /locus_tag="OQM47_000674"
     CDS             complement(397..1296)
                     /gene="nhaR"
                     /locus_tag="OQM47_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1478949.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1362..2528)
                     /gene="nhaA"
                     /locus_tag="OQM47_000675"
     CDS             complement(1362..2528)
                     /gene="nhaA"
                     /locus_tag="OQM47_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1478950.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3114..3266
                     /gene="mokC"
                     /locus_tag="OQM47_000676"
     CDS             3114..3266
                     /gene="mokC"
                     /locus_tag="OQM47_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1478951.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3370..4500)
                     /gene="dnaJ"
                     /locus_tag="OQM47_000677"
     CDS             complement(3370..4500)
                     /gene="dnaJ"
                     /locus_tag="OQM47_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1478952.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4589..6505)
                     /gene="dnaK"
                     /locus_tag="OQM47_000678"
     CDS             complement(4589..6505)
                     /gene="dnaK"
                     /locus_tag="OQM47_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1478953.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6882..7286
                     /locus_tag="OQM47_000679"
     CDS             6882..7286
                     /locus_tag="OQM47_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1478954.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7312..8025
                     /gene="msyB"
                     /locus_tag="OQM47_000680"
     CDS             7312..8025
                     /gene="msyB"
                     /locus_tag="OQM47_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1478955.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8174..8740
                     /gene="satP"
                     /locus_tag="OQM47_000681"
     CDS             8174..8740
                     /gene="satP"
                     /locus_tag="OQM47_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1478956.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8775..9362)
                     /gene="mog"
                     /locus_tag="OQM47_000682"
     CDS             complement(8775..9362)
                     /gene="mog"
                     /locus_tag="OQM47_000682"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1478957.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9477..10430)
                     /gene="tal"
                     /locus_tag="OQM47_000683"
     CDS             complement(9477..10430)
                     /gene="tal"
                     /locus_tag="OQM47_000683"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1478958.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10709..12139
                     /locus_tag="OQM47_000684"
                     /pseudo
     CDS             10709..12139
                     /locus_tag="OQM47_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12209..12985
                     /gene="yaaA"
                     /locus_tag="OQM47_000685"
     CDS             12209..12985
                     /gene="yaaA"
                     /locus_tag="OQM47_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1478959.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13138..13434)
                     /locus_tag="OQM47_000686"
     CDS             complement(13138..13434)
                     /locus_tag="OQM47_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1478960.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13648..14934)
                     /gene="thrC"
                     /locus_tag="OQM47_000687"
     CDS             complement(13648..14934)
                     /gene="thrC"
                     /locus_tag="OQM47_000687"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1478961.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPTDFSALRKLMMNHQ"
     gene            complement(14935..15867)
                     /gene="thrB"
                     /locus_tag="OQM47_000688"
     CDS             complement(14935..15867)
                     /gene="thrB"
                     /locus_tag="OQM47_000688"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1478962.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15869..18331)
                     /gene="thrA"
                     /locus_tag="OQM47_000689"
     CDS             complement(15869..18331)
                     /gene="thrA"
                     /locus_tag="OQM47_000689"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1478963.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18357..18476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18412..18477)
                     /gene="thrL"
                     /locus_tag="OQM47_000690"
     CDS             complement(18412..18477)
                     /gene="thrL"
                     /locus_tag="OQM47_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1478964.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18691..19377)
                     /locus_tag="OQM47_000691"
     CDS             complement(18691..19377)
                     /locus_tag="OQM47_000691"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1478965.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19777..19917)
                     /locus_tag="OQM47_000692"
     CDS             complement(19777..19917)
                     /locus_tag="OQM47_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478966.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20013..20729
                     /gene="arcA"
                     /locus_tag="OQM47_000693"
     CDS             20013..20729
                     /gene="arcA"
                     /locus_tag="OQM47_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1478967.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20788..22140)
                     /gene="creD"
                     /locus_tag="OQM47_000694"
     CDS             complement(20788..22140)
                     /gene="creD"
                     /locus_tag="OQM47_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1478968.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22198..23622)
                     /gene="creC"
                     /locus_tag="OQM47_000695"
     CDS             complement(22198..23622)
                     /gene="creC"
                     /locus_tag="OQM47_000695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1478969.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23622..24311)
                     /gene="creB"
                     /locus_tag="OQM47_000696"
     CDS             complement(23622..24311)
                     /gene="creB"
                     /locus_tag="OQM47_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1478970.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24324..24797)
                     /gene="creA"
                     /locus_tag="OQM47_000697"
     CDS             complement(24324..24797)
                     /gene="creA"
                     /locus_tag="OQM47_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1478971.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25008..25877
                     /gene="robA"
                     /locus_tag="OQM47_000698"
     CDS             25008..25877
                     /gene="robA"
                     /locus_tag="OQM47_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1478972.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25874..26521)
                     /gene="gpmB"
                     /locus_tag="OQM47_000699"
     CDS             complement(25874..26521)
                     /gene="gpmB"
                     /locus_tag="OQM47_000699"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1478973.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26573..27093
                     /gene="yjjX"
                     /locus_tag="OQM47_000700"
                     /pseudo
     CDS             26573..27093
                     /gene="yjjX"
                     /locus_tag="OQM47_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27178..27504)
                     /gene="trpR"
                     /locus_tag="OQM47_000701"
     CDS             complement(27178..27504)
                     /gene="trpR"
                     /locus_tag="OQM47_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1478974.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27594..29531)
                     /gene="sltY"
                     /locus_tag="OQM47_000702"
     CDS             complement(27594..29531)
                     /gene="sltY"
                     /locus_tag="OQM47_000702"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1478975.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29742..31409
                     /gene="ettA"
                     /locus_tag="OQM47_000703"
     CDS             29742..31409
                     /gene="ettA"
                     /locus_tag="OQM47_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1478976.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31516..31683)
                     /locus_tag="OQM47_000704"
     CDS             complement(31516..31683)
                     /locus_tag="OQM47_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1478977.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31717..32949)
                     /gene="nadR"
                     /locus_tag="OQM47_000705"
     CDS             complement(31717..32949)
                     /gene="nadR"
                     /locus_tag="OQM47_000705"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1478978.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32970..34352)
                     /gene="radA"
                     /locus_tag="OQM47_000706"
     CDS             complement(32970..34352)
                     /gene="radA"
                     /locus_tag="OQM47_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1478979.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34401..35369)
                     /gene="serB"
                     /locus_tag="OQM47_000707"
     CDS             complement(34401..35369)
                     /gene="serB"
                     /locus_tag="OQM47_000707"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1478980.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35475..36119
                     /locus_tag="OQM47_000708"
     CDS             35475..36119
                     /locus_tag="OQM47_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1478981.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36147..37162
                     /gene="lplA"
                     /locus_tag="OQM47_000709"
                     /pseudo
     CDS             36147..37162
                     /gene="lplA"
                     /locus_tag="OQM47_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37194..37457
                     /locus_tag="OQM47_000710"
     CDS             37194..37457
                     /locus_tag="OQM47_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1478982.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37618..38337)
                     /gene="deoD"
                     /locus_tag="OQM47_000711"
     CDS             complement(37618..38337)
                     /gene="deoD"
                     /locus_tag="OQM47_000711"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1478983.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38394..39617)
                     /gene="deoB"
                     /locus_tag="OQM47_000712"
     CDS             complement(38394..39617)
                     /gene="deoB"
                     /locus_tag="OQM47_000712"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1478984.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39669..40991)
                     /gene="deoA"
                     /locus_tag="OQM47_000713"
     CDS             complement(39669..40991)
                     /gene="deoA"
                     /locus_tag="OQM47_000713"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1478985.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41118..41897)
                     /gene="deoC"
                     /locus_tag="OQM47_000714"
     CDS             complement(41118..41897)
                     /gene="deoC"
                     /locus_tag="OQM47_000714"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1478986.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42155..43696
                     /locus_tag="OQM47_000715"
     CDS             42155..43696
                     /locus_tag="OQM47_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1478987.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43668..44531
                     /locus_tag="OQM47_000716"
     CDS             43668..44531
                     /locus_tag="OQM47_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1478988.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44745..45527)
                     /locus_tag="OQM47_000717"
     CDS             complement(44745..45527)
                     /locus_tag="OQM47_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1478989.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45524..46597)
                     /locus_tag="OQM47_000718"
     CDS             complement(45524..46597)
                     /locus_tag="OQM47_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1478990.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46719..46880)
                     /locus_tag="OQM47_000719"
     CDS             complement(46719..46880)
                     /locus_tag="OQM47_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1478991.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46997..47602)
                     /gene="osmY"
                     /locus_tag="OQM47_000720"
     CDS             complement(46997..47602)
                     /gene="osmY"
                     /locus_tag="OQM47_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1478992.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47995..49584)
                     /gene="prfC"
                     /locus_tag="OQM47_000721"
     CDS             complement(47995..49584)
                     /gene="prfC"
                     /locus_tag="OQM47_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1478993.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49675..50352)
                     /gene="yjjG"
                     /locus_tag="OQM47_000722"
     CDS             complement(49675..50352)
                     /gene="yjjG"
                     /locus_tag="OQM47_000722"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1478994.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50367..50813)
                     /gene="rimI"
                     /locus_tag="OQM47_000723"
     CDS             complement(50367..50813)
                     /gene="rimI"
                     /locus_tag="OQM47_000723"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1478995.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50782..51195)
                     /gene="holD"
                     /locus_tag="OQM47_000724"
     CDS             complement(50782..51195)
                     /gene="holD"
                     /locus_tag="OQM47_000724"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1478996.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51298..52329
                     /gene="rsmC"
                     /locus_tag="OQM47_000725"
     CDS             51298..52329
                     /gene="rsmC"
                     /locus_tag="OQM47_000725"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1478997.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12531 a        14233 c        13180 g        12658 t
ORIGIN      
        1 aaaattatca gtttcatcat ataagatttg actgcatcca gggcgagcaa ggtgaattag
       61 tttttgaacc cagtgacgtt gaatattact ttgaaccagt gacgatacag gagtcaggaa
      121 ccagcatatt aaaaaatgag ttagaaaaca gtgaggacgg tgcgggtcaa ataggctttc
      181 aactctccaa tgatggtacg aatgaaatcc agtatggtaa aagcaattac tactcttttc
      241 agcatcccca tgaaggaagt aatcagattc ctctatttat tcgcccgcgt acatatggaa
      301 acaatgtctc aagcgggcaa ataatgagcc gagtcaaaat cgtggtaatg tacaactaat
      361 ctatctcccc caccttaact ttgggggagc tgccgattaa cgcaccgctg gactaaaaag
      421 cgcagaataa tccgtattgc agattcgctg taccgccggg tgctgaatca tccgctcagc
      481 aaaaatagca tggtactctt ccatcacatt ctcgacgcga ccaatttcta cgacagtttt
      541 atcggcataa aagtcatatg cataaagcgt tggggcaacg aagattgcat tgtgcatcgc
      601 accaaaagct ttcatcaaag cggcatcatc aaactcgccg aggatttcta cgtttaatcc
      661 ctgggagtta aaccagttaa gcaatttgcg ccctaacatt gaacgtcgcc caggaatcaa
      721 aagtcgccgt tcttccagac aagccgggaa cggtttttct ggtggtggat ttgtacacca
      781 gaaactcacg ccacattcgc caattctcac ggagaacagg ccttcctgct gcgtagagtc
      841 tatcggacag tcagaaatga tcatatccag tttatgctga cttaattgct ccagcagcat
      901 ttcgtgggtg gattcgaagc agcgaagatg aatgggctcg ccttctacca ctgcggcgtt
      961 aagtacgcta ctgaccaggc gtttggaaag tgcatcagcc acgccaacgt caaacaataa
     1021 attggattct ttgcgatagt tcacaatatc cagcatttcc tggcttaagg tgaacatttt
     1081 atcggcatag cgatagacca gttctcccag ctcgctgggt tcgagaccac gtcccttgcg
     1141 tttaaataat ttgccttgca ggcgctcttc cagcgctcga atctgtccgg taatggtttg
     1201 tggagttaaa taaagcgcct ccgctgcgcc aaccacggaa ccttctttat agacatgcca
     1261 gaagtaatac aagtggttgt aattgatatg agacatgctc atttctctcc ctgataacaa
     1321 tgaaaaggga gccgtttatg gctccccggt aaaccgtcct gtcaaactga tggacgcaaa
     1381 cgaacgcgta accagctgta tccaattacc gccgaagaga tagaaccgac caggataccg
     1441 agtttcgccc agttaatcag ttctggatct acgctaccaa aggccaggct ggcaataaag
     1501 atagacatag taaaaccgat accgcacagg atccccacca ccataatttg ctgataagtc
     1561 gttccctcag gcagatgcgc cagtttcaaa cgcagcgcca accagcagaa cagactaatc
     1621 cccagcggtt tgccaatcag caagccagcg atgatcccca atggcagaat ggaggtcaag
     1681 ccatccagcg tgacgccttg cagtgaaacg ccagcattag caaatgcaaa cagcggcaaa
     1741 atcagatacg ccacccacgg gtgcaacaca tgctccagtc gcttcgctgg agaacgccca
     1801 tgcttctctt tcaaaggaat aaagaagccg acaattaccc ccgccagagt tgcgtgaacc
     1861 cccgatttca acaccgcagt ccacaacacc acgccaacaa gaatatagac gcccgtgcgg
     1921 cgtacaccac acagattcaa taccgcgagt accgcaattg ctacagccgc gacgccaagg
     1981 gaggccatcg ataagtcatt agtgtagaac aatgcgatga taatgatggc cccaagatcg
     2041 tcgataatag ccagagccat caaaaagatc ttcagcacta acggaacacg acttcccaac
     2101 agcgccagta caccaagtgc aaaagcaatg tcagtagccg ccgggatcgc ccacccttcg
     2161 cgggtaatcg gatcggcata gttaaaagcc agatagagta atgccggcac aatcatccca
     2221 ccaatagcgg cgataactgg aaatgcggcc tggcgtaagc tggctagcga tccttgcatc
     2281 agttcacgtt taacttccag accgactaac aggaaaaata ccgccatcag cgcatcattt
     2341 atccataaca gcatgttttt gttgatttcg agtgaaccaa cccggagctg aaccggcgtc
     2401 tccagaaagt cgtgatacca tccactggtt gcgccgctgt tggccataat catcgccagg
     2461 atagcggcaa tgataagaat aatgcctccc gaggcatcac tgctaaagaa tcgatgcaga
     2521 tgtttcactt tttatttctc tttcaggtga atagatcgtt tttactattt taccccgcac
     2581 gaatcatcgt taaaatcaga ttatagtgta ggaataattc gttttttacg agccattgaa
     2641 gaatttgcgc catcagcggt gagatgcgtt tttgacggct gtaagcgctc acgagtgacc
     2701 ctaagcccag ataatcatag cttaaggtga ggattcctga gggattaatt aggaaaagta
     2761 ataattaatt tgttgtcagt cggcgatgct tgttaggcca atatttcaaa aataatccag
     2821 cttgtcgtca ataattgcga tgaagagata aattaaagtt aaataattaa atcattaaaa
     2881 aattacgcca caaaaatgcg aggcgtcttc aggattagaa tcgataaaac aaaatgggtt
     2941 aaagagagcg atataatagc ggcgggtgct tgaggctgtc tgtctcaggc attagctgaa
     3001 cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc tcctatatgc
     3061 tgaacacatg tagagtgcct cttactgacc gtaaggtcaa ggagaagaga gcaatgaagc
     3121 agcataaggc gatgattgtc gccctgatcg tcatctgtat caccgccgta gtggcggcgc
     3181 tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg gaggttgctg
     3241 ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc gccactttac
     3301 aggtgctcgc atatcttcaa cgcaccctat ttttacccag gcctgcccac gggcaggctt
     3361 ttggggaggt tagcgggtca ggtcgtcaaa aaacttcttc acaccatcaa agaagctctt
     3421 tgagcgcggg ctgttgtgct cgccggttgg gccaccgaag ctttcttgca gctcttgcag
     3481 cagctgtttc tgcttttcgt tcaggcctac cggtgtttcg acgacaacgc ggcacagcaa
     3541 atcaccctgt gcgccaccgc ggacagactt gacgccttta ccgcgcatac ggaatagctt
     3601 accggtctgg gtttcgccag gcactttcag tttgacgcga ccatcaaggg tcggtacttc
     3661 gatttcgcca cccagcgccg ccatagcgaa gttgatcggg acttcgcaat acaggttgtt
     3721 gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc tgaacgtaca gatcgcctgc
     3781 cggtgcgcca tgctcgcccg cttcaccttc gcccgcaaga cggatgcggt ctccagtgtc
     3841 cacccctgcc gggattttaa cggacagcgt tttgctgcgc tcaacacgac catgaccatg
     3901 acatttgttg cacggatctt tgatcagcgt accgcggccc tgacagtgtg gacaggtctg
     3961 ctgtacagcg aagaatccct ggcgcatctg cacctgacca gaaccatgac aggtcggaca
     4021 ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg caaacgtcac actcttccag
     4081 agtcggaatg cggatctctt tggtcacgcc acgtacagct tcttcgaggg tgagctccat
     4141 gttatagcgt aaatcagcac cgcgcgccgc acgttgacga ccacgtccgc cgccaaaaat
     4201 atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg ccgccgccaa aaccaccgcc
     4261 gcccatgcct ccttgctcaa acgcagcatg accatactga tcgtatgccg cacgtttttg
     4321 cgagtcggtc agaacttcat aagcttcctt gatctcttta aatttcgcct cggcctcttt
     4381 gtcaccctgg ttacggtccg ggtggtattt catggccagg cgtttgtagg cctttctgat
     4441 ttcacgctct tccgctgttt tggaaacgcc taaaatctcg taataatctt gcttagccat
     4501 cttttttaaa ttgcccctag atgaatgcac gggcggagag gaaattcccc ttcgcccgtg
     4561 tcagtataat tacccgttta tagggcgatt attttttgtc tttgacttct tcaaattcag
     4621 cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc atcagcaccg gcagtctgct
     4681 gctgggcatg ttgctgctgg gcgatttcca tcagtttctg ggaaacctgt gccagttcct
     4741 gcattttcgc ttcgatagcg gctttgtctt cacctttcag agcagtttcc agtgcagtca
     4801 gcgcagactc gatagcagtt ttgtcgtcag ccggcagttt gtcgcctgct tcttcaacct
     4861 gcttacgggt gctgtgcagc agatggtcgc cctggttgcg agtctgtacc agctcttcaa
     4921 acttacggtc agcttcggcg ttagcttctg cgtcgcgtac cattttctgg atttcatctt
     4981 cgttcagacc agaagaagcc ttgatggtga tcttctgctc tttaccgctg tttttatctt
     5041 tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa ggtaacttcg atctgcggca
     5101 tgccgcgcgg tgccgggttg ataccatcta ggttgaactg acccagagat ttgttatcag
     5161 ccgcacgttt acgttcaccc tgcagcacat ggatggttac cgcagactgg ttgtcttcag
     5221 cggtagagaa cacctggctg tgcttggtcg ggatagtggt gtttttcgcg atcagcgtcg
     5281 tcatcacacc gcccatggtt tcgataccca gagacagcgg ggtaacgtcc agcagcagta
     5341 cgtcttttac gtcaccagtc agaacaccac cctgaacagc agcaccgatt gctacagctt
     5401 cgtccgggtt aacgtcttta cgcggctctt taccaaagaa ctcagcaact ttcttctgaa
     5461 ccattggcat acgagtctga ccaccaacga ggataacgtc gtcgatatca gatacggaca
     5521 ggccagcatc ctgcagtgca actttcagcg gctcaatgga acggtttacc agatcttcaa
     5581 ccaggctttc cagtttcgca cgagtcactt tgatgttcat gtgtttcgga ccggtcgcgt
     5641 ctgcagtgat gtatggcagg ttaacgtcgg tctgctgagc ggaagacagt tcgattttcg
     5701 ctttttctgc cgcttctttc aggcgctgca ttgccagcgg atcgttgcgc aggtcaatgc
     5761 cctgatcttt cttgaattct tcaaccagat agttgatcag acggctgtcg aagtcttcac
     5821 cacccaggtg ggtatcaccg ttggttgcca gaacttcgaa ggttttttcg ccgtcaactt
     5881 cgtcgatttc gataatagaa atatcgaaag taccaccccc caggtcataa accgcgatag
     5941 tacggttgcc agtgcctttg tccagaccgt aagccagcgc agctgcggtc ggttcgttga
     6001 tgatacgttt tacttccaga ccagcgatac ggcctgcgtc tttggttgcc tgacgctgag
     6061 catcgttaaa gtatgccggt acggtgataa cagcttcagt taccggttca cccaggtaat
     6121 cttcagcggt tttcttcatt tttttcagca cttcagcaga aatctgcggc ggtgccattt
     6181 tctggccttt aacttcgacc catgcgtcgc cgttatcagc agcaataatt ttgaacggca
     6241 tgatggaaac atcacgctgt acttcttcgt cctggaagcg gcggccaatc aggcgtttaa
     6301 tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg tttagccggc tgaccaacca
     6361 gagtttcacc atcctgggta taggcaatga tagaaggcgt ggtgcgatcg ccttcggcgt
     6421 tctccagcac gcgaggaatg gtgccatcca taatcgctac acaagagttg gtagtaccca
     6481 ggtcgatacc aattatttta cccatctaaa cgtctccact atatattcgg tcatcatgtg
     6541 gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac tgcccaattt catttttttg
     6601 cagactcact cactgcggtt gactactaaa tggggtcgta acccacgtca tcaaggggga
     6661 gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg atatggcagg acaagacgta
     6721 aaattgacag caattttatt catataaaga atgaatcgca gccaaggcgt tcatcaaaaa
     6781 attgtaataa aaagaaaaga ttacgtgcct gaatcttctc tttatcagca gtaaactagt
     6841 gggtattcat ccccctacct cttcccacta agagaatcct tatgaaatcc gtttttacga
     6901 tttccgccag cctggcgatt agcctgatgc tgtgctgcac ggcgcaggca aacgaccata
     6961 aactcctcgg cgtcattgca atgccgcgta acgaaaccaa cgatctggcg ctgaaacttc
     7021 ctgtttgtcg cattgtgaaa cgcatacaac tctctgccga ccatggcgat ttacagttaa
     7081 gtggtgcatc ggtttatttc aaagccgccc gtagcgccag tcagagcctg aatattcctt
     7141 cagaaataaa agaagggcaa accactgact ggatcaacat taacagcgat aacgacaata
     7201 aacgctgcgt ctcaaaaatc accttttccg gtcatacggt gaactcatcg gatatggcca
     7261 cgctgaaaat tatcggcgac gactaacgcc agaatataga agccacaaaa aatgaatgtt
     7321 aattacctga atgattcaga tctggatttt cttcagcatt gtagtgagga acagttggca
     7381 aatttcgccc gattgctcac ccataatgaa aaaggcaaaa ctcgcctctc cagcatactg
     7441 atgcgcaacg aactgtttaa atcgatggaa gggcatcccg agcaacatcg ccgcaactgg
     7501 cagctgattg ccggagaatt acagcatttt ggtggcgata gtatcgccaa caaactgcgc
     7561 ggacacggta aattgtatcg ggccattttg ctcgatgttt caaagcgatt gaagctgaaa
     7621 gccgacaaag agatgtctac gtttgaaatt gagcaacagt tactggaaca atttctgcgt
     7681 aatacctgga agaaaatgga cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc
     7741 agggtgaatg agctggaaga gctgctgccg ctgctgatga aagacaaatt attggcaaaa
     7801 ggtgtgtcgc atttgctttc cagccaactg acccgcattt tacgcaccca cgcagcaatg
     7861 agcgtacttg ggcatggttt gctgcgcggc gcggggctgg gaggccctgt gggcgcagca
     7921 ttaaatgggg ttaaagcggt cagcggcagc acctatcgcg tgacgattcc agccgtactg
     7981 caaatcgcct gcctgcgccg gatggttagc gccactcagg tctgataccg gaaaccgggg
     8041 aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg attattatgc
     8101 cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat tcgatgatga
     8161 tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg gcctgatggg
     8221 cttcggcatg accaccattc tgcttaacct gcacaacgtg ggttatttcg ctctggacgg
     8281 tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg ctggtctgct
     8341 ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg gttctttctg
     8401 gctgacgctg gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg cgccaaatgc
     8461 acagttcctt ggtgtctacc tgggtctgtg gggcgtattt acgctgttta tgttcttcgg
     8521 cactctgaaa ggcgcgcgcg tcctgcaatt cgttttcttt agcctgaccg tgctgtttgc
     8581 cctgctggcg atcggtaaca ttgccggtaa tgccgcgatc atccactttg ccggctggat
     8641 tggtctgatc tgcggtgcca gcgcaatcta tctggcgatg ggtgaagtgc tgaacgagca
     8701 gtttggtcgc accgttctgc cgattggtga atcccactaa ttgtcttgag atccccccgc
     8761 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
     8821 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
     8881 ctggcaaaaa taccatgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
     8941 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg
     9001 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
     9061 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
     9121 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
     9181 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
     9241 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgctggaat gcctttatcc
     9301 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
     9361 atcaagattt ttccgtttaa acaatctcgt taaacagaat gatacactgc gaagggagtg
     9421 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
     9481 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
     9541 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
     9601 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
     9661 ccttcgctct ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcacagcct
     9721 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
     9781 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
     9841 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
     9901 ggcgagatca ggaacacgcc cgcctccgca caagcacgag cctgagcgaa ggagaacagc
     9961 agggtcaggt tacagttgat accttctttt tccagctgtt ctgcagcacg gataccctgc
    10021 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
    10081 ttgatcaggc gttttgcttt cgcaattgac gcttcagtgt cataggaaag acgcgcatca
    10141 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
    10201 agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
    10261 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
    10321 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
    10381 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
    10441 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
    10501 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
    10561 actgcgttga tatcgcccac catttttata caaaacctca tgtatgctac gcagaagtta
    10621 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
    10681 atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
    10741 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
    10801 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
    10861 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
    10921 cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gaccaggtgc
    10981 cgtcttttgg atgtgggttg ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc
    11041 ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
    11101 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
    11161 catcgcctac ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag
    11221 tttttctttt gattttcctc cgctggtgac gggcattatt ctcgctgtct ttgctctgct
    11281 ggcaatcact cgtggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
    11341 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
    11401 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
    11461 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
    11521 tgaggtggga atgggatcga cgccaaacgc ggcagcggca gctgcatcct ggcctccgca
    11581 tccggcagca caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
    11641 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
    11701 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt
    11761 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
    11821 cgaaaacaat atcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
    11881 ctgcaccttc gcaacggtca tcggcggcac cttgctaagc cttccgctga tgtggcaact
    11941 ggcagatatc ataatggcct gcatggctat taccaatttg accgccattt tactgctctc
    12001 gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
    12061 ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctccgg acgcgtggga
    12121 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
    12181 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
    12241 tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc
    12301 ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
    12361 catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt
    12421 cacgccggcg aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct acaccggctt
    12481 gcaggccgaa accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
    12541 ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat
    12601 ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat
    12661 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
    12721 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
    12781 ggtgttcctc gatgagaaga acggcaaatt taagatcatc agcttctacg ctaagaaagc
    12841 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
    12901 tggttttaat agcgaaggtt acttctttga tgaagattcc tccagcaatg gcgaactggt
    12961 gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct
    13021 tatccggcct acaaaagcat gcagattcaa tatattgcag aattaacgta ggcctgataa
    13081 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
    13141 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg
    13201 gcgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
    13261 tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg
    13321 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat
    13381 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
    13441 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg
    13501 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgataat ttctcctttt
    13561 tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc
    13621 cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc
    13681 agaaaaatcg gtgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc
    13741 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
    13801 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac gcagcgcacg
    13861 atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt
    13921 ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt
    13981 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
    14041 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac
    14101 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga atgacttcgc
    14161 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
    14221 gcgcgcctcc tgcggcagct gcgcaacagc ttcaaagtag tagcaaatct gcgccagcaa
    14281 acggctgatg ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc
    14341 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
    14401 ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg
    14461 tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgcagc
    14521 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcatccg caatatgggt
    14581 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
    14641 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
    14701 cgcgcgcacg cgctcttcca ggatttcctg tgggatttca tcaccaataa acgccgagag
    14761 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
    14821 gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
    14881 ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt
    14941 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
    15001 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
    15061 tagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc
    15121 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat
    15181 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
    15241 gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
    15301 agcccgggct tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc
    15361 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
    15421 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag
    15481 ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccgcaat gttcattcat
    15541 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac cgatcggcat
    15601 attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc aaaaacgctc
    15661 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
    15721 gagtttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
    15781 accatcaacg ggtgccaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
    15841 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
    15901 gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
    15961 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
    16021 ctttgaacag cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt
    16081 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
    16141 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg
    16201 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
    16261 ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc
    16321 gcgggtccgg ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca
    16381 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
    16441 tcaccaattc atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
    16501 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
    16561 gatgatagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac
    16621 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
    16681 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
    16741 gctctttggc ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag
    16801 tgagcagagc cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca
    16861 gccagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
    16921 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
    16981 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
    17041 caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
    17101 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccgtcaccg
    17161 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
    17221 cagctcgcac acagtcgctt tgtggaacgc agaaactgat actgtattcg gaagatgatt
    17281 gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg cgcgccgcca
    17341 tgccaaccat ccctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
    17401 tggaaatgcc cttgaccggt aattcgtctt catcacggct ggcaccaatg agcgtacctg
    17461 gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
    17521 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
    17581 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc
    17641 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
    17701 cggagtagtc ggaaccgtta cgtccaagca ccaccagttc gcctttttca ttaccggcgg
    17761 tgaaacctgc catcaacacc atgtgatcag ccggaatgcg gcttgccgca atacggcggg
    17821 tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagc agtttttcga
    17881 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
    17941 tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg cactgcccca
    18001 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctgatcg acgaaagttt
    18061 tcaattgcgc cagcgggaac cccggctggg cggcggcgag tcccgtcaaa agttcggcaa
    18121 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg tttttttcaa
    18181 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
    18241 cctgcctggc attgctttcc agaatatcgg caacacgcag aaaacgttct gcatttgcca
    18301 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
    18361 aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc
    18421 accgttacct gtggtaatgg tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga
    18481 tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg cctatattgg ttaaagtatt
    18541 tagtgaccta agtcaataaa attttaattt actcacggca ggtaaccagt tcagaagctg
    18601 ctatcagaca ctcttttttt aatccacaca gagacatatt gcccgttgca gtcagaatga
    18661 aaagctgaaa aatacttact aaggcgtttt ttatttggtg atattttttt caatatcatg
    18721 cagcaaacgg tgcaacattg ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa
    18781 ccagtcgacc agttttatgt catctgccac tgccagagtc gtcagcaatg ccatgactcg
    18841 ttcgcgtaaa gcttgcagtt gatgttggtc tgccgttgtg tcacttttcg ccggttgttg
    18901 tattaatgtt gctaattgat agcaatagac catcaccgcc tgccccagat tgagcgaagg
    18961 ataatccgcc accatcggca caccagtaag cacgtcagcc aacgctaact cttcgttagt
    19021 cagcccggaa tcttcgcgac caaacaccag cgcggcatgg ctcatccatg aagatttttc
    19081 ctctaacagc ggcaccagtt ctactggcgt ggcgtagtaa tgatatttcg cccgactacg
    19141 cgcagtggtg gcgacagtga aatcgacatc gtgtaacgat tcagccagtg tcgggaaaac
    19201 tttaatatta tcaataatat caccagatcc atgtgcgacc cagcgggtgg ctggctccag
    19261 gtgtgcctga ctatcgacaa tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc
    19321 cgctgcccca atattttctg ctctggcggg tgcgaccagg ataatcgtta tacgcatatt
    19381 gccactcttc ttgaccaaat aaccgcgaac agggtgatca ctgtcaactt attacgcggt
    19441 gcgaatttac aaattcttaa cgtaagccgc agaaaaagcc ctttacttag cttaaaaaag
    19501 gctaaactat tttctgactg cactaacggt ttaattgtta aaaaatgcta catatccttc
    19561 tgtttactta ggataatttt ataaaaaata aatctcgaca attggattca ccacgtttat
    19621 tagttgtatg atgcaactag ttggattatt aaaataatgt gacgaaagct agcatttaga
    19681 tacgatgatt tcatcaaact gttaacgtgc tacaattgaa cttgatatat gtcaacgaag
    19741 cgtagtttta ttgggtgtcc ggcccctctt agcttgttat gttgctgtta aaatggttag
    19801 gatgacagcc gtttttgaca ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat
    19861 gatgttgttg acgttgatgg aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt
    19921 acgccgatca tgttaatttg cagcatgcat caggcaggtc agggactttt gtacttcctg
    19981 tttcgattta gttggcaatt taggtagcaa acatgcagac cccgcacatt cttatcgttg
    20041 aagacgagtt ggtaacacgc aacacgttga aaagtatttt cgaagcggaa ggctatgatg
    20101 ttttcgaagc gacagatggc gcggaaatgc atcagatcct ctctgaatat gacatcaacc
    20161 tggtgatcat ggatatcaat ctgccgggta agaacggtct tctgttagcg cgtgaactgc
    20221 gcgagcaggc gaatgttgcg ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa
    20281 ttctcggcct cgaaatcggt gcagatgact acatcaccaa accgttcaac ccgcgtgaac
    20341 tgacgattcg tgcacgcaac ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag
    20401 aacgtcgtag cgttgaaagc tacaagttca atggttggga actggacatc aacagccgtt
    20461 cgttgatcgg ccctgatggc gagcagtaca agctgccgcg cagcgagttc cgcgccatgc
    20521 ttcacttctg tgaaaaccca ggcaaaattc agtctcgtgc tgaactgctg aagaaaatga
    20581 ccggccgtga gctgaaaccg cacgaccgta ctgtagacgt gacgatccgc cgtattcgta
    20641 aacatttcga atctacgccg gatacgccgg aaatcatcgc caccattcac ggtgaaggtt
    20701 atcgcttctg cggtgatctg gaagattaat cggctttacc accgtcaaaa aaacggcgct
    20761 ttttagcgcc gtttttattt ttcaacctta tttccagata cgtaattcat cgtccgttgt
    20821 aacttcttta ctggctttca ttttcggcag tgaaaacgca taccagtcta tattacgagt
    20881 cacaaacatc atgccggcca gcgccaccac cagcacactg gttcccaaca acagcgcgct
    20941 atcggcagag ttgagcagtc cccacatcac tccatccagc aacaacagcg cgagggtaaa
    21001 caacatgcta ttgcgccagc ctttcaatac cgcctgcaaa taaataccgt tcattaacgc
    21061 cccaatcaga ctggcgatta tccatgccac ggtaaaaccg atatgttcag aaagcgccag
    21121 caagagcaaa taaaacatca ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa
    21181 acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata aaagtcagtg caatcagcag
    21241 aatggcgtac ttagtcgccc ggtccgttaa ttggtattga tcggctggcg tcgtcactgc
    21301 gacgctaaac gcggggaagt tttcccagcc ggtatcattg cctgaagcaa aacgctcacc
    21361 gagattatta gcaaaccagc tgctttgcca ttgcgcctga aaacctgact cgctagcttc
    21421 ccgtttggct ggtagaaaat cacctaaaaa actgggatgc ggccagttgc tggttaaggt
    21481 catttcgcta ttacgcccgg caggcaccac agaaagatcg ccggtaccgc ttaaattcag
    21541 tgccatattc agctgcaggt tctgcttccg ccagtcccct tcgggtaaag ggatatgcac
    21601 gccctgcccg ccttgctcta acccggtacc gggttcaatg gtcagtgccg ttccgttaac
    21661 ttcaggcgct ttcaccacac caataccacg cgcatccccg acgctaatca caataaaggg
    21721 cttgcctaag atgatatttg gcgcgttgag ctcgctaaga cgcgaaacat cgaaatcggc
    21781 ttttagcgtt aaatcactgt gccagacctg accggtataa atccctatct tgcgttcttc
    21841 cacgttctga ttgccatcaa ccatcaatga ctcaggtaac caaaaatgga taaaacttcg
    21901 tttccgctcc acggttttat cctcttcctg caccgtataa agctcggtca caggaatagc
    21961 gataagcggc ccaacgagtt tttgcggccc gctggtactt tggcgaatcg catcttccac
    22021 atcgctacgg taatcggcac gttcgacaat cacttgccga atcagcatta tcggaatcaa
    22081 caacagcaat actgcaccaa acaaggtggt cattttccag aacagggggg atttcaacat
    22141 agtcttctcc tttgcaatgg cagcaggata cgaaggctat gtgggaagaa tttgaagcta
    22201 tgtgaagtga cggtgaagtc gtagcgaggc cagcacgcca ccttcctgca cgttgcgcag
    22261 cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg aacgccaacc ccagaccgct
    22321 gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc
    22381 gtagtcagga ataccactac cggtatccag caccttaagc gtgacgtgtt cctgatccac
    22441 ttcggcgctt agcgttatgc aaccgctctc gggagtaaaa tcgatggcgt tatccagtaa
    22501 attccccagc gcctgatcca gtaacgccgg ttcagcagca acattaacct cggtgggcat
    22561 aacatgcaaa gtgatgtttt tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg
    22621 aaataatgcc gccacatcaa cagcagtcag aacgacttcc tgacgattct ccagtcttgc
    22681 ctggcgtagt aacgtttcca ccagcgcctg cattcgcgca ttttgtgtca gaatgttgtc
    22741 agtaaaacga gccactactt caggcggcgg accttcgcgt aaaatttccg ccgcgccacg
    22801 aatcgccgcc agtgggcttt ttagctcatg ggttaacgca taaacatact gctcaatata
    22861 gtttttccct tccagcttca cgcgcatact ttccagcgcc tgcgcgagtt tacgcagctc
    22921 gctactaccg agatcgggaa gaggaacggg cttattgtca gtgacggaat cagcatagcg
    22981 agtgagccgg gcaatggagc ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc
    23041 aatccccaac aaaatggcgc tggcccataa aattcgccgc tcgctgcgtt taatgaccgg
    23101 agccatcgcc gcgttcggtt tgcctacgct caaaacgcca ataagccgcg agccgcccat
    23161 aatcggcgcg gcaacataca tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct
    23221 gcgcgcacca tactgaccac gcaacgttag ccagacgtca ttccagcgcg aataatcctg
    23281 tccaacggct ttatttgccg aatcgaacaa tactttgcca tgcgcatcgg tcatatagac
    23341 atgatattca ttgcgcactt tgttaatgcc accaatattg gcgcgaaacg ggcgatgttg
    23401 tagctgatta aacgcctgcg ccagttgccc atgcgttggg tccccagaga gcaaatcggg
    23461 acgcgccagc tccgcaagta acgttgccgt gtcgatcaac gtcccctccg ttgctcttcg
    23521 cacgcccggt ttaacttctt tgacaaaaat agccagtacg aaccaggccg ccaccgccac
    23581 cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc attacaagcc cctcaggcta
    23641 tatcccatgc cgcgatgagt attaatcggt gaaagatcgg ggttgatggc gcgcagcttg
    23701 gcacgcagcg ttttaatgtg gatatcgacg gtgcgatcgt aggtgtcctg cgcatcttcc
    23761 cacacgctat ccattagttg ctggcgggac cagacgcggc ctggcgactt gagtaacgtc
    23821 ttcagcaata aaaactcata ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc
    23881 gccgcgggtt cattcaattc aaaatgacca atacggatga cgggagacgg cgtcgagaac
    23941 ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca cttcgcgggg tgaaaacggt
    24001 ttagccacat agtcgtcagc gccaatttcc agcccaagca ggcgatcgac ctcttcactt
    24061 cgggccgtca ggaacagtac aggtaacgcc ggatggagcg ccagtaactg gcggcacaat
    24121 tcaaagccac tgatatcagg caaaccaacg tcgagaatca tgacgtcggg aacctgctgg
    24181 cgagctttat ccagcaccgg caaacctcgc tcaaagacct cgacggcaaa accttcctgc
    24241 tgcaacatgt agaccagcgt gtcggctatc ccttgctcat cttccactaa ccagaccgtt
    24301 tcccgttgca taaatcgcct ctgttattgc cgccacggca tgacaggaac cgcgctaatc
    24361 gcgtttttcg gcgaaccttc tacaactttg tcggagtaag ccagataagc gagcgcgttg
    24421 cgtttggcgt cataaaagcg cacgacctgt aacgacttaa agaccaggga cgtgcgtttt
    24481 ttgaatacta cctcgccctg agctttgccg tttttaatac gatccgacag ttcaatcggc
    24541 ccgacttgct gacaagaaat ggccgcatcg gaggtatctt ctgccaggcc caatccccct
    24601 ttaataccac cggttttcgc ccggctcaca taacaggtga catttttcac atcgggatcg
    24661 tcaaaggctt ccacaacaat tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc
    24721 gaaccaatct cttctgcatg agccaatggc cccagcatta tcaggcttaa agacaggatc
    24781 aaatgcttgt atttcatatt gttaccattt ttatctatta cacgagcaat tagttcgtca
    24841 cggtaaataa catattgatt aagaacaaaa tctcaattct tttatttccg tcaagcccta
    24901 aaacatactc tactaagaaa aaaacactga atgctaaaac agcaaaaaat gctattatcc
    24961 aattacctga tgtcaggtgc tcgttgttga aaggatgagg atattttatg gatcaggccg
    25021 gcattattcg cgacctttta atctggctgg aaggtcatct ggatcagccc ctgtcgctcg
    25081 acaatgtagc ggcgaaagca ggttattcca agtggcactt acagagaatg tttaaagatg
    25141 tcactggcca tgctattggc gcgtatattc gtgctcgtcg tttgtcgaaa tcggcggtcg
    25201 cactacgcct gactgcgcgt ccgattctgg acatcgcgct gcaataccgc ttcgactctc
    25261 aacagacatt tacccgcgca ttcaagaagc agtttgccca gactcctgca ctttaccgcc
    25321 gttctcctga atggagcgcc tttggtattc gcccgccgct acgtctgggt gaattcacaa
    25381 tgccagagca caaatttgtc accctggaag atacgccgct gattggtgtt acccagagct
    25441 actcctgttc gctggagcaa atctctgatt tccgccatga aatgcgttat cagttctggc
    25501 acgattttct cggcaacgcg ccgaccattc cgccggtgct ctacggcctg aatgaaacgc
    25561 gtccgagtca ggataaagac gacgaacaag aggtattcta taccaccgcg ttagcccagg
    25621 atcaggcaga tggctatgta ctgacggggc atccggtgat gctgcagggc ggcgaatatg
    25681 tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga gtttatcctg acggtatacg
    25741 gaacgtgcat gccaatgctc aatctgacgc gccgtaaagg tcaggatatt gagcgatact
    25801 acccggcaga agacgccaaa gcgggagatc gcccaattaa tctgcgctgt gaactgctga
    25861 ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg ggcgtccaga tgcgagatgt
    25921 cccctgcggt ttcaacgacc cagccagacg ccagccacag gctttcctga taatccacgc
    25981 gcgaaataga acagttacgc agacgtaagc ggcgctctgc ccatgctggt aatccgagaa
    26041 tcgtactcac cagacatccc agtgcaatac catggctcac cagcagcggt cggcttccct
    26101 gcggtaagtc ccggcaagat tccagtgccg cattgacgcg atcgctgagc tcctgcattg
    26161 actcgccttc aggaatacgc ccgtcaacgg tgccattgac cagctgccga cgccagttct
    26221 cttcttcttc ggtcagagaa tcgatatgtc ttttttccag cacacccatg tttaattcac
    26281 gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg ggcgatgatt tccgccgtac
    26341 gccgggtgcg tcctaaatcg ctactgataa tatgcgtaat gccaagctct ttggcacggg
    26401 ttgccacctg catcgcctgt tgctcacctt tggcggtcag cgggctgtca gactggccct
    26461 gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg gactaggtat acctgtaaca
    26521 tgcttttttt ccgtatactg ctgtaacgtt aattttctga gtaatgctga ttatgcacca
    26581 agttgtctgt gcgaccacca atcccgctaa aattcaggcc attctgcagg catttcacga
    26641 gatcttcggc gaaggatcct gccatattgc atccgttgcc gtcgagagcg gtgtaccgga
    26701 acaacccttt ggcagtgagg aaacgcgcgc tggcgcacga aatcgggtag caaatgcccg
    26761 ccgtttactt ccagaggctg atttttgggt ggcgattgaa gctggcattg atggcgacag
    26821 cactttcagt tgggtggtga ttgaaaacgc cagccagcgc ggcgaggcgc gttcggcaac
    26881 tttgccgtta cctgcggtga ttctggaaaa agttcgtgaa ggcgaggcgc ttggtcccgt
    26941 gagtcgcgtt ataccggtat tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt
    27001 accgccggga aactcactcg cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt
    27061 ccgtttcaca atgccgtgta tcaggcattg taggacggat aaggcgttca cgccgcatcc
    27121 gacattcggt gcacgatgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca
    27181 atcgcttttc agcaacacct cttccagcca ctgacgcagc tccacgggcg cggctttcag
    27241 gctgttagat ccacgcgtaa tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg
    27301 ctgactcatt tcgccacgca acagctcttc gacaatacgc acgcgagtcc ccaacgcttc
    27361 gcgctcatct ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt tttggtaggc
    27421 attcttaagc aggtcgacaa aacgtaacca ctcctggtga cgctgttctg ccatcgctgc
    27481 tgaatagggt gattgttggg ccataatatg tcgccattgt tagcggggga agcaaaatga
    27541 ctcccccggt tgtactcgct aaagagtacg atagcatatc ataaacgtgc ggatcagtaa
    27601 cgacgtcccc attccgtggc gctcatcaac gtcggtttat cccccatgaa atagcggtag
    27661 taagcatcat aagccagcac gttcttcaca taaccgcgcg tctcggagaa cggaatactc
    27721 tcgacaaatg ccacagcgtc gatacgcccg gcgctgttgc caagccaggt tcgcacccgt
    27781 ccaggtccgg cgttataagc tgctgaggag aaaatacggt tattgccaaa ctgctgataa
    27841 acatattgca ggtaactggt gccaatgttg atgttcgttt ccggatccag caattgccca
    27901 gggctgctat aaccaggaat agagaacatc ttcaccgtat gggtcgctgt accaggcata
    27961 atctgcatca ggccgctggc ccctaccggt gatttcactt tcggattcca ggcgctctcc
    28021 tgacgggcaa tcgccatcgc atagctttgc gggatctctt tcccactggt gtaacgtttg
    28081 aaaagatcgt tgtaagccag cgggaatcgc tcttccagat gatcccaaag cttcccggcg
    28141 atcgttgcct gaacgctaag atcccaccat tggttattga aagcataccg agccagttga
    28201 gcctgctctg tttttgactt gctcttcacc agattggccc actcgctacg cgcggtatta
    28261 tcgagattcc agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg agtcagggtg
    28321 ctgtcaacat tctgcggcgc tttatcaatc ttcagctcat actcttcacc gatgcgttgt
    28381 gctgcaacca tcgggtagaa accacgctgt tgtatgagtt gatgcaaaat ctctttcgct
    28441 tcagcttcac gtccgcgttc cagcaataaa tccgcctgcc agtaacgcca ttcatctttc
    28501 tcttttgctt ccatcggcag acgcgccagc caggtattca ggccgcgacg atcgccggtg
    28561 ccaagcgcca ttcgcacacg gcgttcaatc aacgacgtgg attgcgagcg cataatggcg
    28621 tcatcgcgcc atttcgcctg ctcatcggtg acatcattgc ccatcaaacg ccaggcgacg
    28681 atatcgcgca gctcctgaat ctgatcttca ttaagctgct gcgcctgggc aagcgacggg
    28741 atcatcagcc gtgcattttc ggcatcctgc cgcgccacac tggcaaacgc caccgccgcc
    28801 atttgacggg taaaatcggt cgcgccggtt gtacgcgcga aggtcagtac cgtattaggg
    28861 ttgttcgcca gtgaaatgat tgccgaggcg atagtctggt aatcggcagg catctgccct
    28921 gccagcactg ttaccaggcc tgtgttaccc gctttcatcg ccagacggat acgctctaaa
    28981 tacgccagcg gatcttgttt acctgacgca cgccagacgc taaataactt gtcacaggcg
    29041 ttaggctggc tcttgccggt tagccacagc tctttcgccc cttgccaggc ttcttcactc
    29101 tgcccggtgt tccatttcgc atagtagtaa ttacattgcg cttcggtagt tccgggcttt
    29161 tccgggctaa aggctaacaa gccacgccag tcttcacgcc gcgccagttc attgacgaaa
    29221 cgagattgca gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa gttagtgacc
    29281 gtcaccgccg gttgattcat caggtcatcg gtgatctggc ggtattccag gtagggataa
    29341 agcggataat ccttcagtcc aggcatcatt tgttcgacca catccatctg tcgattatcc
    29401 caggcctgct tgatttgcgc gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc
    29461 acgctgctga ccgtcagcag acagacaccg gcagccaaca gccgccaggt aacttgtttg
    29521 gctttttcca caagcgcatc ctctaagtgt aaatacatca atgcagcgtc atgccgcgta
    29581 atagatttat gctaaccagt cattgccgtt tacgccacgt tacggacact tttttacttt
    29641 tactgcgaag gtgatcgacc aactgggatt aggcggggaa acaggctaca cttcgccttt
    29701 gaaaactcat catcacgata aacatagagg cgaagtccaa cgtggctcaa ttcgtttata
    29761 ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa aacatctctc
    29821 tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg ggtaagtcca
    29881 ccctgctgcg cattatggcg ggcattgata aagacatcga aggtgaagcg cgtccgcagc
    29941 cagacatcaa gattggttat ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc
    30001 gtgagtccat tgaagaagcg gtttcagaag tggttaacgc cctgaaacgc ctggatgaag
    30061 tgtatgcgct gtacgccgat ccggatgccg attttgacaa gctggccgct gaacaaggcc
    30121 gtctggaaga gatcattcag gctcacgacg gtcataacct gaacgtacag ctggagcgtg
    30181 cggcggatgc gctgcgtctg ccagattggg acgcgaaaat cgctaacctc tccggtggtg
    30241 agcgtcgtcg cgtggcgttg tgccgcctgc tgctggaaaa accagacatg ctgctgctcg
    30301 acgaaccgac caaccacctg gatgccgaat ccgtggcgtg gctggaacgc ttcctgcacg
    30361 acttcgaagg caccgtggtg gcgattactc acgaccgtta cttcctcgat aacgttgcag
    30421 gctggatcct cgaactggac cgcggtgaag gaattccgtg ggaaggtaac tactcctcct
    30481 ggctggagca gaaagatcag cgtctggcgc aggaagcttc acaagaagcg gcgcgtcgta
    30541 agtcgatcga gaaagagctg gagtgggtac gtcagggaac taaaggccgc cagtcgaaag
    30601 gtaaagcacg tctggcacgc tttgaagagc tgaacagcac cgaataccag aaacgtaacg
    30661 aaaccaacga actgtttatt ccacctggac cgcgtctggg cgataaagtg ctggaagtca
    30721 gcaacctgcg taaatcctac ggcgatcgcc tgctgattga tgacctgagc ttctcgatcc
    30781 cgaaaggggc gatcgtcggg atcatcggtc cgaacggcgc gggtaaatcg accctgttcc
    30841 gtatgatctc cggtcaggaa cagccggaca gcggcaccat cactttgggt gaaacggtga
    30901 aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc gtttgggaag
    30961 aagtttccgg cgggctggat attatgaaga tcggcaacac cgagatgcca agccgcgcct
    31021 acgttggccg ctttaacttt aaaggggttg atcagggtaa acgtgttggc gaactttccg
    31081 gtggtgagcg cggtcgtctg catctggcga agctgctgca ggttggcggc aacatgctgc
    31141 tgctcgacga accaaccaac gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc
    31201 tgctggagtt cccgggctgt gcgatggtta tctcgcacga ccgttggttc ctcgaccgta
    31261 tcgccacgca catcctggac taccaggatg aaggtaaagt tgagttcttc gaaggtaact
    31321 ttactgagta cgaagagtac aaaaaacgca cgctgggcgc agacgcgctg gagccgaagc
    31381 gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg cgcgaacgcc
    31441 ttatccggcc tacaaaacca gcaatttcaa tacgttgtaa aactgtaggc ctgataagac
    31501 gcggtaagcg tcgcatcagg cattgagcac cgaatgccgg atgcggcgtg aacacctttt
    31561 ccggtctaca acagccttag catcctcaat ttcgtggcgg gtcacccgtg caggtttttt
    31621 caaaccttgt ttaatctcga cagcctcttc cagagaggtt ttcaactcat caaaaaaact
    31681 cattttatac ctcgcctttg agcagtttcg tcacggttat ccttgctccc ccatcatctc
    31741 ccgcaccagc tccacgcagc gcaggaaacg gctgtcgtaa tcgtcctctt caacccgcac
    31801 gaattcgata ttgttctctt ccagcatctc caccagcaag ttctggaact ctttgcgatc
    31861 caccgaactg ccgaggctgc gtaaaccatc cgccacccac ggcgtgttgt tctccagcag
    31921 gatcaccaga tcgaaacggt attcatcaat cagcgcctgc acgaacggat gctcacgccc
    31981 ttcgtacttt ttgcagaacg cctgggtagt gacaaaatcg gtgtcaataa acgccacttt
    32041 attggcgtat ttcactgcaa aatcaatgta ttgtgcgtga cccaacgcga ttttatcgta
    32101 gtcggaatac tgcaatgcga tctcatcacc gccgaggtgt gaaaagacat aatcgcggcc
    32161 atattcccac gcactggtgg tgttgaagat attggcaagt ttgtttacca gggtggattt
    32221 accgctcgac tcgccgccaa ggatcgccac ggtacgcaca aaaaacggct tcacttcggt
    32281 aggaatatat tcccagtagc ggaacgggtt ttcgcggatc tgcgcaccgc tgatactcat
    32341 aaaggtacgt ttcggatcga ccagcaccgt ctcgatcccc agatgttcca tatactgtgg
    32401 cgcatcggct tcttccgagg tgtagatcag gtccggctgg atcccctttt cagccataaa
    32461 ctttttgatg ccgttgctcc acacatccca gccatgcgga tacggctcca tgccctcttc
    32521 gttgaaagca tgaatacgga tatttttctg atatttaaag gtttgcaata gccagcgcag
    32581 acgatccggc accgtgggct gctgcgacat ggcgctgtct tcaaacaacg cgcggtcgcg
    32641 ggtatcgtca aaacccataa tgatatgcag ctcgtcaacc tggctacagg cgcgctggat
    32701 aaggtagata tgtccggtat gcagcgggta gaacttaccg aatacgacac caatggtttt
    32761 cttctgccgg ggaaattcaa gccccaaaaa acggtgcaat gcctccagct tttgcgcgct
    32821 ggggctttta attttggcat tcagtaactg gcttaaatac cctttggtca taccgctggc
    32881 atcagctacc tgctgtagcg tgcagccctg ttgcttgatg gcagttttca ggtaatcaaa
    32941 tgacgacata tctccctccg tatctctcat tataagtcgt cgaacacgct aagcgcgtcg
    33001 gagagttttt taacgccaaa aatctgcatc ccttccggcg cttttttcgg tacgttagcc
    33061 gccggaacaa tcgcccggcg aaaaccgtgt ttcgccgctt cagaaatccg ctcctggcca
    33121 ctgggcaccg ggcggatctc ccctgccagc ccgacttcac caaacaccac cagatcctgt
    33181 ggcagcggtc tgtcacgcag gctggaaacc atcgccagca gtaacgctaa gtcggcgctg
    33241 gtttcggtta ccttcacgcc gccgaccacg ttcacaaaca catcctgatc ggccatttgc
    33301 agaccaccgt gacggtgcaa gacagccagc aggattgcca gacggttttg ttccagcccc
    33361 actgccacgc ggcgcggatt cgccattatc gagtgatcga ccagcgcctg aatctccacc
    33421 agcaacggac gcgttccttc ccacaccacc atcacggagc tgccggaggt cacttcatct
    33481 ccgcgactta agaaaattgc cgaagggttg ctgacttcac gcagcccctg ttcggtcatc
    33541 gcgaagacgc ccagctcatt caccgcgccg aagcggtttt tatggctgcg taaggtgcga
    33601 aaacgggagt cggcatcgcc atccagaagc accgaacagt cgatacagtg ttccagcact
    33661 ttcgggccag ccagcgaacc atctttggtt acgtgtccaa ccatgacaat cgccacaccg
    33721 cgcgttttgg cgaaacgcgt cagataagcc gccgtttcac gcacctgcgc cacgctacca
    33781 ggcgatgatt gtacatccgc catatgcatc acctggatcg agtcaattac catcagcttc
    33841 ggttgctctt cttcggcaat caggcagatc tgctcgatgc tggtttccga caacatattg
    33901 agattgtcag tcggcaggcc aaggcgatga gcgcgcattg ccacctgttg cagcgactct
    33961 tcgccggtga catacagcgt tttcatctgc tgggccagtt tgcacagcgt ttgcagcaac
    34021 agcgtggatt tccccgcacc agggttaccg ccaatcagaa tggcacttcc tggcaccacg
    34081 ccgcctccta gtacgcggtc gaactcttta aatccggtgg aaaaacgcgg cagctcttca
    34141 aggctgatat cggagagttt ctggactttt gccaccccgg cgctaccggc atagccgctg
    34201 agacgctcgt tacgcgccac cgttggcgac gcagcaagac gcacctcggt gatggtgttc
    34261 caggcatgac aggcactgca ctgcccctgc cagcgcggat aatcggcccc gcattcatta
    34321 caaacaaagg cgcgttttgg agcttttgcc acggtgtacc tcgttaatgc tgtgccgccc
    34381 gcaggatgac gggcaggcaa ttacttctga ttcaggctgc cagagaggat gcagaatacc
    34441 cccatcaggt cagcgtgacg gatggtgact tccgcctttt cattcacttt tggcttggca
    34501 tggtaggcaa tccccagccc tgctgctttg atcatcggca ggtcattagc tccatcgcca
    34561 atcgccacgg tctgcgccag cgggatttca tactcctgcg cgaggcgagt cagagttttc
    34621 gctttgtact gcgcgtctac gatgtcgccg atcacattgc cggtaaattt accgtccatg
    34681 atctccagtt cattggctac cacggctgtc aggcgcagct tgtcgcgcag gtattcagca
    34741 aagaaagtaa agccgccgga ggcaatcgcc actttccagc ccagcgtttc cagcttgagc
    34801 accagctgcg ttaagcctgg catcagcggc agattttcac gcacctgttg cagaatattg
    34861 gcgtcagcgc ctttcagcgt cgccacgcgg ctgcgcaggc tggcggtaaa atcgagttcg
    34921 ccgcgcatcg cccgttcggt tacctccgcc accatctcgc ccgttccggc cagcttggca
    34981 atttcatcaa tacattcaat ctggatggcg gtggaatcca tgtccatcac cagcaaaccc
    35041 ggcgtgcgca ggtgcgggat tttccccagc ggggcgacat ccagctgcgc ttcgtgggcc
    35101 aggcgtgtag cccgtgcggt gagtgaacct gccagacgaa tcacctgata atcttccacg
    35161 caccaggcgg caacaatcac catcgccgca cccagtttgc tctggtattg ggtcagacgc
    35221 tgtttatcca gcccacgacc atacagcagc cagccgctac gacctgcgtg gtaatccagt
    35281 ggcatcactt catcaccact taatgaaaga ggcagacccg gccataaaga gacatcttca
    35341 ggcaggtcgc accaggtaat gttaggcatt aaggctcctg taaaatcgtt cgaagcaggg
    35401 aaaataacgc atgaggctac cttgtatcca ttgcttctgg caacattaag tctcaaattt
    35461 tcaaagggtg gaagatggct cgcacaaaac tgaaattccg gctacatcgg gcagtgattg
    35521 tcctgttctg tcttgccttg ttagtggcac tgatgcaggg ggcgtcatgg tttagtcaaa
    35581 accaccagcg acagcgtaat ccacagctgg aagaactggc ccgcaccctg gcgcgtcagg
    35641 tgacgctgaa cgttgcaccg ctgatgcgta ccgactcacc ggatgaaaaa cgcattcagg
    35701 cgatcctcga tcagttaaca gatgaaagcc gtatcctcga cgcgggtgtg tatgacgaac
    35761 aaggcgatct tatcgcacgt tctggcgaaa gcgtcgaagt gcgcgatcgg ctggcgctcg
    35821 acggtaaaaa agcaggcggc tattttaacc agcagattgt cgagccaatt gcgggtaaaa
    35881 acggaccgct cggctatctg cgcctgacac tcgacaccca tacgctcgcc accgaagccc
    35941 aacaggtgga taacaccact aacattttac gcctgatgtt gctgctctca ctggcaatcg
    36001 gcgtagtgct gacccgcacg ctgctacagg gtaaacgcac ccgctggcag caatcgccct
    36061 tcctgttaac cgccagcaaa ccggtgccgg aagaggaaga aagcgagaaa aaagagtgac
    36121 ccattactac aagaaaggaa atcgttatgt ccacattacg cctgctcatc tctgactctt
    36181 acgacccgtg gtttaacctg gcggtggaag agtgtatttt tcgccaaatg cccgccacgc
    36241 agcgcgttct gtttctctgg cgcaatgccg acacggtagt aattggtcgc gcgcagaacc
    36301 cgtggaaaga gtgtaatacc cggcggatgg aagaagataa cgtccgcctg gcgcggcgca
    36361 gtggcggtgg cggcgcggtg ttccacgatc tcggcaatac ctgctttacc tttatggctg
    36421 gcaagccgga gtacgataaa actatctcca cgtcgattgt gctcaatgcg ctgaacgcgc
    36481 tcggcgtcag cgccgaagcg tccggacgta acgatctggt ggtgaaaacc gtcgaaggcg
    36541 accgcaaagt ctcaggctcg gcctatcgcg aaaccaaaga tcgcggcttc caccacggca
    36601 ccttgctact caatgccgac ctcagccgcc tggcaaacta tctcaatccg gataaaaaga
    36661 aactggcggc gaaaggcatt acttcagtgc gttcccgcgt gaccaacctc accgagctgc
    36721 tgccggggct caccatgagc aggtttgcga ggccataacc gaggcctttt tcgcccatta
    36781 tggcgagcac gtggaagcgg aaatcatctc cccggacaaa acgccagact tgccaaactt
    36841 cgccgaaacc tttgcccgtc agagtagctg agagtggaac ttcggtcagg ctccggcatt
    36901 ctcgcatctg ctggatgaac gctttagctg gggcggcgtg gaactgcatt tcgacgttga
    36961 aaaaggccat atcacccgcg cccaggtatt taccgacagc ctcaaccccg cgccgctgga
    37021 agccctcgcc gggcgactgc aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg
    37081 cgaagcgctg ttggttgact tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg
    37141 gatagcgggg gcggtaaggt aactcgctga ttaaaatatc caaggaagga aatatgatcg
    37201 atctggaaaa ccaggaacgg gagattatca atttaatgtt cagccagcgt atttcctggc
    37261 tagccgccgt gcgtatcagg cataaattat cgcttgctga agtaagtaaa atgttaggta
    37321 tctcgattaa ttctctaaaa cagatcgaaa agacggaacg gcttagctca aacatcaaaa
    37381 gcaaaatggc agaaatttat ggttgtccac cagagttgct gatatgccct tcctggatga
    37441 cggctgaaca taaatgaata gattcgtgaa tagatacttg aatgtttttt attttctatc
    37501 catatgaaaa ataatagtaa atctataaat cagttgctat tgatgaatag atactaaaaa
    37561 gccggagcag tcccccactc cggcttaaca cgacaatcgc ctttcagcga aacacaatta
    37621 ctctttatcg cccagcagaa cggattccag tgcgattttg atcatgtcgt tgaaggtggt
    37681 ctgacgctca gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca cggtgcagat
    37741 ggtcagggct ttcgcgccaa actctgcagc cacgccgtag ataccagccg cttccatttc
    37801 cacgccgagg atgccgtatt tttccatcac gtcgaacatt tcgccgtccg gagagtagaa
    37861 caggtcagcg gagaacaggt tacccacgcg agcatcaacg cccagtgctt tagctgcatc
    37921 tactgcgtta cgcaccatgt caaagtcagc gatagcggca aagtcatggt ctttaaaacg
    37981 gatgcggtta actttggaat cggtgcaggc ccccataccg ataacgacgt cgcgcagttt
    38041 tacgtgcggc agaactgcgc cacaggaacc aacgcggata attttcttca cgccgaaatc
    38101 ggtgatcagt tctttggtgt agatggagca ggacgggata cccataccgt gacccattac
    38161 ggaaattttg cggcctttgt aagtaccagt gaagcccagc ataccgcgaa cgttgttcac
    38221 ttcacgggca tcttcaagga aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg
    38281 catcaaaact acgtcagcga aatcgcccat ttctgcatta atgtgtgggg tagccattgt
    38341 tttatccttt ttgtgacata acaaaggcgg agtcaacgct ccgcccaaat ccatcagaac
    38401 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    38461 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc atataccagt
    38521 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcgca accgtggtca
    38581 gcggtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    38641 tcgaacagtt ccagacccgc agcataaccg gcgacgtcgc ggcggtggcc ccaggaagag
    38701 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    38761 atggtggcgt caaacagcgc gtccaagcca gtcgctttca cttttttggt gataccgcag
    38821 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc
    38881 agtttctgca gcacggtcgg tgctggcggc tcaacagcca agtcgtgacg gttaccggta
    38941 cgctggaagt taccagcttt gtcaccgata aacggacgag cgataacacg accgatattg
    39001 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaga
    39061 ccgaaagttt cttcatggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    39121 ggcttgccgg ttttcatgtg ctcttcgccc agttgatcca gaatgaccgt accggaagag
    39181 tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc cagcagctct
    39241 tgcgggaagc tgttttcgtg atcggagaaa tatccccact caaacagaac cgggacaccg
    39301 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    39361 tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct
    39421 tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg ccctttacga
    39481 ccattatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg
    39541 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    39601 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    39661 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgcgttatcg
    39721 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    39781 gcgaggataa ccgccagcgg acgctgaccg tctacctggt cgcccagacg cgccatatca
    39841 gtaaagccga cgctgtaatc gatggtgtca gatgcctgac gacgaccgcc gcccattgca
    39901 actactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    39961 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    40021 gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    40081 agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc
    40141 aacatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atactcaccc
    40201 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    40261 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    40321 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    40381 ttcacgtcca tcaccagcgc gtccagacct tccgccagtt tcttcgccag gatcgaggca
    40441 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    40501 tcagccggag ccagtgaact ggtctgaccg ataatcgcca cgccgacgtc tttaataatt
    40561 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    40621 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    40681 gctgcgacca tcggccccaa catcagcgaa gtgacatcgc cgacgccgcc ggtcgagtgt
    40741 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    40801 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    40861 atcgcgaggg cggcaatctg cccttcggaa atagtgttgt cgcgaatacc gttgataaag
    40921 aaacgaattt cttcatcgct cagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    40981 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    41041 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    41101 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcgccgtg gcccagcgct
    41161 ttcagcaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atcagcccag
    41221 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    41281 acgccgcccg ccggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    41341 atcatgatgc gagcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    41401 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    41461 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    41521 ttacaggctt tcaccaggtc aaaaccaacc tgctcgtcac ccgccatcag cgcgcggtac
    41581 gggaacacca cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    41641 gcgatttcga tgtcgtcgtt accgtgtggg aagttggtta ccgtagcgat acggatttcc
    41701 ggggtgccct gctctttcag agttttgcga gcaatcggga taaaacgagg atagatacag
    41761 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    41821 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    41881 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    41941 ttgttagtat tctaacatct actccgcaac acacttcgat acacatcaca attaaggaaa
    42001 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    42061 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    42121 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    42181 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    42241 ttttctggca ctggaagcag aaaacaatct gccttaccca cagcttcctg ccgaagcccg
    42301 ccgcgcgctg gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    42361 gcgctatgtc ttacccgatt acgctcgttt tctggcgaac ggttccgaat ggctggagct
    42421 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    42481 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    42541 tgttagaatt ctaacacaaa acgagatcga tgttcgaata aaacgtttct ggcgttacct
    42601 cgacagaacc ctgccagatg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    42661 tacccgcgcg atcttacgtg cagacgcgca ggtttcaccg aacctgacct ttatctacga
    42721 tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct gtgaatgtag
    42781 caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag ggggctacgg
    42841 gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc tggtacgcct
    42901 taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta cgcgcactct
    42961 accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat tcctctatca
    43021 acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta accctgaacg
    43081 ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc tgtgtgagaa
    43141 agaagggatt gccgcgcgct acggtaaaga agccgccgca aatgaagtgg gttatcgcat
    43201 cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct ggcaaaaacg
    43261 cgccatgtta cacgcacagt cggggatcag ttccgatatc ggcaccacgc cgggcgcgcg
    43321 tttgccgtat ggcgatgagc cagatccgat cacccatctg caaactgtcg ccccgcatca
    43381 tgcttattat tattccggca tcagcgacat tctgacgctc gacgaaacca tcaaacgtaa
    43441 cccgcaggca ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc gtgaatttac
    43501 cgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc gtttgtcgga
    43561 tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg gcgaagaagc
    43621 cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg aacagcagat
    43681 gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc agtcgtctgg
    43741 ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg tggacgatgg
    43801 gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg ttgcagattg
    43861 ttgacggcaa agtggtgtgg aacgctgtgg tctgcgagca atgtgatacc tgcctgaaga
    43921 tgtgtccgca acatgccaca ccaatggcgc aatccatgag cgtggacgaa gtgcttagcc
    43981 atgtgcgcaa agcggtgctg tttattgaag ggataacggt aagcggcggc gaagccacga
    44041 cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg caactgcgcc
    44101 atctcacctg tctggtggac agtaacggta tgttgagcga aaccggctgg gaaaaattac
    44161 tcccggtgtg tgacggcgca atgctcgatc tcaaagcatg gggtagcgaa tgtcatcaac
    44221 aattaaccgg acgcgataat caacagatta agcgcagcat ctatttgctg gcagagcgcg
    44281 gcaagctggc ggaactgcgt ttgctgatga tcccaggcca ggtggattat ttgcaacaca
    44341 tcgaagaact ggcggcgttt atcaagggac ttggtgatgt tccggttcgc ctgaacgcgt
    44401 ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg ccggaagacg
    44461 ttgagccgtt ggctgatgca ttaaaggtgc gcggggtgag ccggttgata tttccggcgc
    44521 tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag gcctgataag
    44581 acgcgtcaag cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt
    44641 atccggccta cggttcggca caaacctgta ggcatgataa gacgcgttaa gcgtcgcatc
    44701 aggcattatg cgccgactgc cggatgctgc ctacgaaggg caaactaccc acgaacgtta
    44761 aacaccgcat acgtgttatt aagcaacact tcggcaatct catccgcggg ttccgggcgt
    44821 aactcgcaaa gcacggcaaa cacacgggca gcctgttccg ggcggttagg ctgcccctga
    44881 aaaccgttga gcggcatatc cggcgcatcg gtttccagca acaacgatgc cagcggtaat
    44941 tttgcgatga catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg
    45001 attttgtagc ccagctgtac aaaccgttca gcctgttgct ggctgccaga aaatccgtgg
    45061 acaacaccag cacgcggcaa atcgtggcgt ttaagatgca tcgccagttt gtcgtgcgtg
    45121 cgccgtgaat gcaggatcac cggcagatcg tagcgtttcg ccagtttcag ttgttcgtcg
    45181 agtaaccact gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc
    45241 cccaccgcca ccaccttcgc cggacgcctt tccagcgcct gctgtagctg ctcaagagac
    45301 acatcgctat gtttttccaa cataccagga tgcaagccca atacggcata cagcggttga
    45361 taattttccg ctaacgcctg cacacgggca aaattttccg cctctgtcgc cggaacaatg
    45421 atcttgccta cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccaataaaa
    45481 ggcgggaaat caaaatggca gtgagtgtcg ataaaacggc aaatcaagcc agatcctcat
    45541 tgttaaatgt cgggtcgttc gcctgcggag cgtcgctcac ttcggcaacc agcgtatcgt
    45601 tggcgactgg cgcaggcgga atgacaatcg attccggcgt caccactggc accagatggc
    45661 gggtaagcgg cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc
    45721 ataaacgccc aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac
    45781 tatgtaatgg cttcggcgga taaatttcgg atatccgcag cttgcccggt ggtttctcaa
    45841 taaattgctg aatgtcacga tagctggttt catgatgctg caccagattg accagcggct
    45901 gcaggctact gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca
    45961 tttgtgacgg cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt
    46021 taaccggaat cgcatcactg atcccgccat ccaggtagtt aatgccttcc agcgacactc
    46081 cgctacgata aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt
    46141 tggttggtaa aaagtaattc ggcgcgtagt catcctgacg acaggcgcac atataaaacg
    46201 atttgccgct gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg
    46261 tggcctccac cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat
    46321 cgaaaaattc gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat
    46381 tgcagataaa cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag
    46441 gattaaactg cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc
    46501 cgccgccttc gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg
    46561 caatattacc aagcgttaca ggtattcgct gccccacttc tgccttcctg tttttgttgt
    46621 gaaagaaaga caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg
    46681 gactggcttt ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg
    46741 aacaaactca ctaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca
    46801 gcggtaccgg ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat
    46861 atgatgcccc aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat
    46921 gagcgtgaaa atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa
    46981 agttcgggag gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta
    47041 ccgctttggc gatactttca gcacggtcac tttgtgcctg agaatctacg gtaccggaga
    47101 gctgaaccac gccatcggtg gtttcaactt tcactttacg tgaagggacg atatcgtccg
    47161 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag cccttcaccg
    47221 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacg ccttcaacgc
    47281 ctttcgccac tttcactgcc tcttcggcct gggcctgact ttcaacgaaa ccgctcaggg
    47341 tcacgacttt ttgatcggtt ttgacagaga tatcggtgct cttgatgttg tcatgatcca
    47401 ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    47461 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgcgcg ttgttttccg
    47521 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    47581 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagccta
    47641 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    47701 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga atcagttatt
    47761 aatatcggta gaatgacaat cgacggcttt agatactaat atctacgcat tgaacggtat
    47821 ttaacgccat cagaaatgtc atcactttgt taaatataga tcacaatttt gaaaccgctc
    47881 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    47941 ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc cctggaggca agaattaatg
    48001 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttgac
    48061 catgctggta gcgatgtaag cgaggttatc gccgccatca agcgccagtt ggctttcgtt
    48121 cttacgcttg aactcttcga atttcttcgc gtctgcacat tctacccagc gggcagtggc
    48181 aacgttgact gactcataca ctgcttcaac gttgtattcg ctcttcaggc gcgctaccac
    48241 cacatcaaac tgcagcacac caaccgcacc aacgatcaga tcgttgttgg agattggacg
    48301 gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    48361 cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc
    48421 ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt
    48481 gtgcaggccg aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt cacccgccat
    48541 aaaggtcagc gcgtcggaga tcaccacatc tttcgcagtg cgcacctggc gcagtttcat
    48601 gcctttttca tatttaccgg acaccacacg cataaacgcc acgcggtcgc ggtgtttcgg
    48661 atccatgttg gcctgaattt taaatacgaa gccggtaaat ttatcttcgc tcgcttctac
    48721 ggtacgggta tcggtctgac gcggcatcgg cgcaggcgcc cactccacca ggccattcag
    48781 catatgatca acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc
    48841 cgcaaggaac agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc
    48901 acgcagctgc tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    48961 tttaacaatg cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc
    49021 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaagtgat
    49081 cggtgcgcag ccaattttca gctcgttctc aacttcatcg agcagttcca tcgggtcgcg
    49141 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    49201 ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca
    49261 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcgt gccccggggt
    49321 gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga
    49381 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg
    49441 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    49501 ggtagtttta ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc
    49561 tttcaaataa ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg
    49621 cttgttgaaa acgaaaaatt gcggctattt tacccatcaa cgggggggag gcaatcagtg
    49681 tttacacagg agctgctcca gttcgtgcaa cgaagaaacg gtccaggtgg gcgcgatgcc
    49741 ttctggttgc tcgcgattgt gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc
    49801 accgagaata tcggactcgg cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt
    49861 gcccgcctgt tccagcgcat aatcgaaaat tttcttattc ggtttggcaa cgccaacttc
    49921 ttcggaaatc accagcaaat cgaagtaatc acgcaggccc gtccgttcca gacgcacttg
    49981 ctgcaaggcg ctaaaaccgt tggtgatgat gccgatttta gcgttgctac gaatggcgtt
    50041 aagcagagaa accgcgcccg gcaacggcgt acagatttcc gccatcgcat taataaaggc
    50101 atcgttaagc aagcctgctt caactttcag ccgttcggcc cagctctcga aacgcccgtg
    50161 ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc acccacagtg gcttgttaac
    50221 ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg
    50281 ctgcaggccg gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca
    50341 gtcccacttc attattccac cttgtcttac atactgattg gcaacgccat gatgatggcg
    50401 tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct
    50461 aaactttcgt acagggcaat ggcggcagca tttgaggcac ggacttccag ccatagtgtc
    50521 gccacgccgc gtttttccag ttcgtcgatc agatgttcca gcagcgccct acccagcccc
    50581 tgacgctgat agtcgggatc gaccgcaata ttgaacaatg tagcttcatc cagcaccact
    50641 tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta actgaaagtt gagataacgc
    50701 tcgccctggt tgctggcaaa ggttttttca ctccacggaa aggcgtgggc gcgttgttca
    50761 atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca
    50821 aatttgttgc cataacgcgg cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg
    50881 tgatgccacc tgagcgcctt ccaatgatag cggttcgtca gtacccaacc gccagctgtt
    50941 gcagcgactg ccttgcggca gcatcgcgat tttttctggc gtcagttgca gcacttgatc
    51001 ggggctgacg gttaatgcgc gcagaacatc gctcactaaa ggatcagtca gggcgggaag
    51061 atcgtttgcc accatcacca gacggacgtg tgccgggctc gcaatggcaa tctccccctg
    51121 caacgcgcca gggcgacgca gcgaccactg ggtaatgccc agttgctgta actgccagtc
    51181 tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt
    51241 cgataccgcg ccaacatatg gctataatcg ccgccagtat caattgagga gcattccatg
    51301 tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc
    51361 cgtattctgt ttgccggaga cttacaggat gacctgcccg cgcgtttaga taccgcggcc
    51421 agccgtgctc atacccagca attccaccat tggcaggtgt taagctgcca gatgggggat
    51481 aacgcccgtt ttagtctggt cgccacggcg aatgatgtcg cagattgcga tacgctgatt
    51541 tactactggc cgaagaacaa accggaagcc cagttccagt tgatgaattt actttctctg
    51601 ctgccagtgg ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgct
    51661 gagcagatgc tggcagatta tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc
    51721 ctctattttg gtcgtctgga aaaacagcca gtatttgatg cggataaatt ctggggcgaa
    51781 tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg
    51841 gatgtcggta gccagttgct gctctcgacg ttaactccgc acacgaaagg taaagtgctg
    51901 gatgtcggct gtggcgcggg tgtactttca gttgcctttg cgcgccattc gccgaaaatt
    51961 cgtctcacct tgtgcgatgt ctctgccccg gcggtagaag ccagccgcgc aacacttgca
    52021 gccaacggtg ttgaaggtga agtctttgcc agcaacgtct tttccgaggt gaaaggttgt
    52081 tttgatatga tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctggatgcg
    52141 gcgcaaacgc tgattcgtgg cgcggtgcgt catcttaata gcggcggcga gctgcgaatt
    52201 gtagcgaacg ccttcctgcc ttacccggac gtgctggatg agacatttgg cttccatgaa
    52261 gttatcgcgc aaaccgggcg cttcaaggta tatcgcgcca ttatgacccg ccaggcgaag
    52321 aaaggttgag tatctgatat ctgtcgccgc ataccgtgtc ggatgcggcg tgaacgcctt
    52381 atccgaccta caaaacctgc acgttagccc ttcgtaggcc agataagacg cgccagcgtc
    52441 gcatctggca tctgcccccg catgcctacc aaacctgcac gctagtttcc tgatggacat
    52501 ttttccagca attacacatc aggcgataat taactattga cgaaaagctg aaaaccacta
    52561 gaatgcgcct ccgtggtagc aattcttttt aagaattgat gg
//
LOCUS       DAJVPT010000012        52521 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVPT010000012 DAJVPT010000000
VERSION     DAJVPT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..146)
                     /locus_tag="OQM47_000726"
     CDS             complement(<1..146)
                     /locus_tag="OQM47_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS1478998.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVL"
     gene            complement(159..1940)
                     /gene="gcl"
                     /locus_tag="OQM47_000727"
     CDS             complement(159..1940)
                     /gene="gcl"
                     /locus_tag="OQM47_000727"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1478999.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2030..2845)
                     /gene="allR"
                     /locus_tag="OQM47_000728"
     CDS             complement(2030..2845)
                     /gene="allR"
                     /locus_tag="OQM47_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1479000.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2923..3405)
                     /gene="allA"
                     /locus_tag="OQM47_000729"
     CDS             complement(2923..3405)
                     /gene="allA"
                     /locus_tag="OQM47_000729"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1479001.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3635..4561
                     /gene="allS"
                     /locus_tag="OQM47_000730"
     CDS             3635..4561
                     /gene="allS"
                     /locus_tag="OQM47_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1479002.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4630..5724
                     /gene="mnmH"
                     /locus_tag="OQM47_000731"
     CDS             4630..5724
                     /gene="mnmH"
                     /locus_tag="OQM47_000731"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1479003.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6437..8852)
                     /locus_tag="OQM47_000732"
                     /pseudo
     CDS             complement(6437..8852)
                     /locus_tag="OQM47_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(8849..9535)
                     /locus_tag="OQM47_000733"
     CDS             complement(8849..9535)
                     /locus_tag="OQM47_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1479004.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9506..10128
                     /gene="tesA"
                     /locus_tag="OQM47_000734"
                     /pseudo
     CDS             9506..10128
                     /gene="tesA"
                     /locus_tag="OQM47_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10118..10927
                     /gene="ybbO"
                     /locus_tag="OQM47_000735"
     CDS             10118..10927
                     /gene="ybbO"
                     /locus_tag="OQM47_000735"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1479005.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            10988..11842
                     /gene="cnoX"
                     /locus_tag="OQM47_000736"
     CDS             10988..11842
                     /gene="cnoX"
                     /locus_tag="OQM47_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1479006.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(11905..12684)
                     /gene="fetB"
                     /locus_tag="OQM47_000737"
     CDS             complement(11905..12684)
                     /gene="fetB"
                     /locus_tag="OQM47_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1479007.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12671..13348)
                     /gene="fetA"
                     /locus_tag="OQM47_000738"
     CDS             complement(12671..13348)
                     /gene="fetA"
                     /locus_tag="OQM47_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1479008.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13494..14411
                     /locus_tag="OQM47_000739"
     CDS             13494..14411
                     /locus_tag="OQM47_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1479009.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            14408..14866
                     /locus_tag="OQM47_000740"
     CDS             14408..14866
                     /locus_tag="OQM47_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1479010.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(14867..15274)
                     /gene="cueR"
                     /locus_tag="OQM47_000741"
     CDS             complement(14867..15274)
                     /gene="cueR"
                     /locus_tag="OQM47_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1479011.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(15399..16691)
                     /locus_tag="OQM47_000742"
     CDS             complement(15399..16691)
                     /locus_tag="OQM47_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1479012.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(16694..17626)
                     /gene="glsA"
                     /locus_tag="OQM47_000743"
     CDS             complement(16694..17626)
                     /gene="glsA"
                     /locus_tag="OQM47_000743"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1479013.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            17888..20392
                     /gene="copA"
                     /locus_tag="OQM47_000744"
     CDS             17888..20392
                     /gene="copA"
                     /locus_tag="OQM47_000744"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1479014.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(20607..20948)
                     /locus_tag="OQM47_000745"
     CDS             complement(20607..20948)
                     /locus_tag="OQM47_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1479015.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            21086..21880
                     /locus_tag="OQM47_000746"
     CDS             21086..21880
                     /locus_tag="OQM47_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1479016.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            22084..22563
                     /gene="ybaK"
                     /locus_tag="OQM47_000747"
     CDS             22084..22563
                     /gene="ybaK"
                     /locus_tag="OQM47_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1479017.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(22600..24252)
                     /gene="ushA"
                     /locus_tag="OQM47_000748"
     CDS             complement(22600..24252)
                     /gene="ushA"
                     /locus_tag="OQM47_000748"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1479018.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            24470..25690
                     /gene="fsr"
                     /locus_tag="OQM47_000749"
     CDS             24470..25690
                     /gene="fsr"
                     /locus_tag="OQM47_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1479019.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            25928..27604
                     /locus_tag="OQM47_000750"
     CDS             25928..27604
                     /locus_tag="OQM47_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1479020.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27737..29041)
                     /gene="gsk"
                     /locus_tag="OQM47_000751"
     CDS             complement(27737..29041)
                     /gene="gsk"
                     /locus_tag="OQM47_000751"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1479021.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            29193..30152
                     /gene="aes"
                     /locus_tag="OQM47_000752"
                     /pseudo
     CDS             29193..30152
                     /gene="aes"
                     /locus_tag="OQM47_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(30149..31111)
                     /gene="hemH"
                     /locus_tag="OQM47_000753"
     CDS             complement(30149..31111)
                     /gene="hemH"
                     /locus_tag="OQM47_000753"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1479022.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(31243..31887)
                     /gene="adk"
                     /locus_tag="OQM47_000754"
     CDS             complement(31243..31887)
                     /gene="adk"
                     /locus_tag="OQM47_000754"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1479023.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            complement(32068..33942)
                     /gene="htpG"
                     /locus_tag="OQM47_000755"
     CDS             complement(32068..33942)
                     /gene="htpG"
                     /locus_tag="OQM47_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1479024.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(34052..34657)
                     /gene="recR"
                     /locus_tag="OQM47_000756"
     CDS             complement(34052..34657)
                     /gene="recR"
                     /locus_tag="OQM47_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1479025.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(34657..34986)
                     /locus_tag="OQM47_000757"
     CDS             complement(34657..34986)
                     /locus_tag="OQM47_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1479026.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(35039..36964)
                     /gene="dnaX"
                     /locus_tag="OQM47_000758"
     CDS             complement(35039..36964)
                     /gene="dnaX"
                     /locus_tag="OQM47_000758"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1479027.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(35642..35706)
                     /gene="dnaX"
                     /locus_tag="OQM47_000758"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(37093..37644)
                     /gene="apt"
                     /locus_tag="OQM47_000759"
     CDS             complement(37093..37644)
                     /gene="apt"
                     /locus_tag="OQM47_000759"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1479028.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(37797..38174)
                     /locus_tag="OQM47_000760"
     CDS             complement(37797..38174)
                     /locus_tag="OQM47_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1479029.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            38244..38771
                     /gene="priC"
                     /locus_tag="OQM47_000761"
     CDS             38244..38771
                     /gene="priC"
                     /locus_tag="OQM47_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1479030.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38785..38946
                     /gene="rsmS"
                     /locus_tag="OQM47_000762"
     CDS             38785..38946
                     /gene="rsmS"
                     /locus_tag="OQM47_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1479031.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(39158..42520)
                     /gene="mscK"
                     /locus_tag="OQM47_000763"
     CDS             complement(39158..42520)
                     /gene="mscK"
                     /locus_tag="OQM47_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1479032.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42648..43295)
                     /gene="acrR"
                     /locus_tag="OQM47_000764"
     CDS             complement(42648..43295)
                     /gene="acrR"
                     /locus_tag="OQM47_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1479033.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            43437..44630
                     /gene="acrA"
                     /locus_tag="OQM47_000765"
     CDS             43437..44630
                     /gene="acrA"
                     /locus_tag="OQM47_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1479034.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            44653..47802
                     /locus_tag="OQM47_000766"
     CDS             44653..47802
                     /locus_tag="OQM47_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1479035.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            48348..48722
                     /gene="tomB"
                     /locus_tag="OQM47_000767"
     CDS             48348..48722
                     /gene="tomB"
                     /locus_tag="OQM47_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1479036.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            48748..48966
                     /gene="hha"
                     /locus_tag="OQM47_000768"
     CDS             48748..48966
                     /gene="hha"
                     /locus_tag="OQM47_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1479037.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            49138..49689
                     /gene="maa"
                     /locus_tag="OQM47_000769"
                     /pseudo
     CDS             49138..49689
                     /gene="maa"
                     /locus_tag="OQM47_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            49805..50275
                     /locus_tag="OQM47_000770"
     CDS             49805..50275
                     /locus_tag="OQM47_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1479038.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            50439..51989
                     /gene="pdeB"
                     /locus_tag="OQM47_000771"
     CDS             50439..51989
                     /gene="pdeB"
                     /locus_tag="OQM47_000771"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1479039.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(52031..52384)
                     /locus_tag="OQM47_000772"
     CDS             complement(52031..52384)
                     /locus_tag="OQM47_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1479040.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT        12519 a        13814 c        13505 g        12683 t
ORIGIN      
        1 agtacctgtt ttaattcttc aatgtcgtag tcataaggaa acataaattc aacgccgcga
       61 aaaccacact gcgcagcttt ctcaaaacgg gcgagaaaat catattcacc aaataacatc
      121 gataaattag cagagaaacg taacatcatt tatctccctt attcatagtg catgaagcag
      181 gtttcagtcg gtgcatccgc tgcgttatcg gcgatatctt caaattccat gacgttatcc
      241 agttcgctgc ccatcgaaat attggtcaca cgctcgagaa taacttccac gactaccggt
      301 acccgatatt gcgccattaa ggctttcgcc tgttcaaagg ctggcgcaat atcttccggt
      361 ttgaagaccc gaatagcttt acaacctaaa ccttccgcta cttttacgtg gtcaacaccg
      421 tagccattca cttcactgga gttgatattc tcgaaagcga gttgcacgca gtagtccatg
      481 tcaaaagcgc gttgtgactg acgaatcagg ccgagatagg cgttattcac cagcacatgg
      541 atgtacggaa tgttgaactg cgcgccaacg gctaactctt caatcaggaa ctggaagtca
      601 aagtcgccag aaatcgccac cacattgcgt ttcggatcag cggcacaacc ccccagcgcc
      661 gccggaatcg tccagcctaa cggaccagcc tgaccacagt tgatccagtg gcggtcttta
      721 aagacatgca gcatttgtgc cgcagcgatt tgtgacagac caatagtggt gacataacaa
      781 acatcgcgac caaaggcttt gttcatctct tcatacacgc gctgcggttt caccggcacg
      841 ttgtcgaagt gggttttgcg cagcaaagtg cgtttacgct gctggcagtc ggcgacccat
      901 tctttacgac acggcagacg acccgctttt tgcatctcct gcgccacttc aaccagcagt
      961 gtcagcgccg ctttagcatc agagacaatg ccgagatccg gacacagcac gcgaccaatt
     1021 tgcgtcggct caatatcaat atgaacgatt ttgcgccctt cggtgtattt ctctaccgaa
     1081 ccggtatgac ggttagcaaa acggttaccg ataccaaaca ccatgtcgga cgccagcaac
     1141 gttgcgttac cgtaacgatg cgcggtttgc agaccgacca tcccggccat cagttcatga
     1201 tcgtccggga tacagcccca gcccatcagc gttgggatca ccggaacgct ggtcagttca
     1261 gcaaactgtt gtaacagtgc agctgcgtca gcattgatta ccccgccccc ggcaacaatc
     1321 accggacgtt cggcctggat taacatttct acagcttttt cgatctgcat acggctggca
     1381 gcaggtttgt agaccggcag cggttcgtac atgtcaggat caaactcgat ttccgcaacc
     1441 tgaacgtcga acggtaaatc caccagtacc ggacccggac gaccagaacg catcaggtga
     1501 aatgcctgtt gcagcacgcg aggcaccagc gccgcttcac gaactgtaac cgccattttg
     1561 ctgaccggtt tagcaattgc ttcaatatct acggcctgaa aatcttcttt atgcagacgg
     1621 gcgcgcggtg cctggccggt aatgcacaga ataggaatgg aatcagcaga agcggaatag
     1681 agcgcggtga tcatgtccgt gcccgcagga ccggaagtcc ccagacatac gccgatattc
     1741 cctgccgttg cgcgggtata accttccgcc atgtgcgaaa caccttccac atgacgcgcc
     1801 agaatgtgac gaataccgcc gtgcttacgc atcgctgagt agaacggatt aattgcagct
     1861 cccggaacgc cgaaggcggt ggtgatacct tctttctcca gcacatacat tgccgcgtca
     1921 acggctctca tttttgccat tttattccta cctctattag ttggaaaaat tttccaactt
     1981 tcaaattttg tcaaaaaaac ccgctccgca gagcgggatg cgacagacac tatggatgtg
     2041 ctttcagtcc caacgccgtg ctgatatcgc gggcggtgtc tctgaccagc tcaccctgac
     2101 tgacaaaacg atcttctgtc agtcttgatg aaggcccgga gatggagata gcggcaacaa
     2161 ctctaccgac atcatcgtaa attgctgaag ctatgcaatt cagacctaca acatgctctt
     2221 ctttatctac ggtatagccc agttcacgcg cttgttccag gtccttcagc aaggtgggca
     2281 tatccacaag cgtagttggc gtaaactgct gcaaaccggt ttgcagaatg atgctcatca
     2341 actcctcttc cgccagcgga taaagcagcg ctttgcccgc accggaagca tgcagcggca
     2401 gacgactgcc cagtggcgca cacatcctga ccatcgattt acactctaac tgaccaatta
     2461 ataccgcttc attgccgtta cggatcgcga cattgaccgt ttcgccggaa agtaacatca
     2521 ggcggcgcat aaacggcccg gcgacggaga ggacatcgcg gttatggatg tacgccgcac
     2581 cgacgttaaa gacacctaat cctatatgcc accagcctaa ttgactgtcc tgatagacaa
     2641 aatccgctgc ttgtaaaacc ttcagcaagc gaaaggtcgt ggagagcggc aaatccagat
     2701 tgagagaaat atcgctaacc gacgaacttc ccccactttt ttccagatat tgcagaatcg
     2761 caattccccg ctctaacgcc tgtgcgccct tctgtgccac aggctccgcc tgtcctggcc
     2821 tgccgcgccg tctaacttcc gtcataccta cgttaactcc gacctacaag tgagcataaa
     2881 caatgaacaa ttagtttatc ttatgcaagt cggttgcagg cgtcacgcaa aacagagttc
     2941 ctgttcagga atactttcaa catcacagtt gtcactgccg ccgcgatcga tggtcagaaa
     3001 atcggtgacg cgctgccagg cgaaaagtgg gtgatgccag acgttacgat ggtaattcac
     3061 cccctgttca ccgttggtga taaacgcccg cagcgttgac aggtctggtt tgtcgtcgcc
     3121 taacgccacg accaccacaa acacttcacc tttcattggg ataaaggcct gagtgcccag
     3181 cggatgacgt tcgagttcgt gaatggtcag cggcagattc gccggttgcg cgcggttaat
     3241 gctgataagc gtacggtctt gctcaagaat ctcaaccagc gccaaatcgt ggtaacgctc
     3301 caccaggcca ttgttaatat ggaaaaaatc ccgttgctgc gtttcgatta cgtcgccata
     3361 agcactaaag gcttcctgac ttaacggtaa tacctgaagt ttcatctttt tgtccttgta
     3421 acataattcc gttcaaccac cgccgaccgg tcagcttggt ttcaataaca ggcaatctaa
     3481 aaccttaaga gaacaacatt aatacaaaag ttggaatgtt tttctaaaac tagatttagt
     3541 ccacaaattg aacaggatta tcatgtaaaa atactgataa atcagaggat aagaaaatca
     3601 cgcaacttgc ggatttaaaa ggggatacct gaagatgttc gatccagaaa ccttgcggac
     3661 tttcattgcg gttgctgaaa caggaagttt ttcaaaagcg gcagaacgat tatgtaaaac
     3721 cacggcgacg atcagttatc gcattaaact tctggaagag aataccggag tagcgctgtt
     3781 tttccgtacg actcgtagcg taacgttgac agcggctggc gagcatctac tttcccaggc
     3841 cagagactgg ctgagctggc tggaaagtat gcccagcgag ctgcaacagg tgaatgatgg
     3901 cgtggaacgc caggtgaata ttgtcatcaa caacctgctc tataaccccc aggccgtcgc
     3961 ccagttgctg gcgtggctga atgagcgtta cccctttacc cagtttcaca tctcccgaca
     4021 aatctatatg ggcgtctggg actcgctatt gtacgaaggt ttttcgctgg ctatcggggt
     4081 cacgggaact gaggcgctgg caaatacctt tagtcttgat cccttaggat cggtgcaatg
     4141 gcgatttgtc atggcggcgg atcatccgct ggcgaacgtt gaagagccgc taacagaagc
     4201 gcagttgcgt cgctttccgg cggtcaatat tgaagacagc gccggcacct taaccaaacg
     4261 cgtcgcctgg cgattgccgg ggcaaaaaga gattattgtt cccgatatgg aaacgaaaat
     4321 cgccgcccat ctggcgggtg ttggcattgg ttttttgcca aaatcacttt gccagtcaat
     4381 gatcgataat caacaactgg tcagccgggt aatcccaacg atgcgccctc cttcgccatt
     4441 gagtctggca tggcgcaaat ttggcagctg caaagcggta gaagatattg tgaccttgtt
     4501 tacccaacac aggccggaaa tcagcggatt tttagaaatt ttcggcaccc cacgcagtta
     4561 aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc gcaagctgat ttgaggttca
     4621 cttttaacta tgcaagagag acacacggaa caggactatc gtgccctgct gattgctgat
     4681 acgcccatta ttgatgttcg cgcccctatc gagtttgagc aaggcgcaat gcccgccgct
     4741 atcaatctgc cgttaatgga taacgatgaa cgcgcctccg ttggcacctg ctataaacag
     4801 caaggctcag acgcagcgct ggcgctgggg cataaactgg tggcgggtga aattcgtcag
     4861 cagcgtatgt acgcctggcg ggcagcgtgc ctgcaaaatc cgcaaggtat tctctgctgc
     4921 gcccgtggcg gtcaacgttc acatattgtg caacgctggc tgcatgaagc ggggattgat
     4981 tatccgctgg tggaaggcgg ttataaggca ctgcgccaga ccgcgattca ggcgaccatt
     5041 gaactggcac aaaaaccgat agtgctgatt ggcggttgta ccggcagcgg taaaacgcta
     5101 ttagtgcaac aacagccaaa cggtgttgat ctggaagggc tggcgcgtca tcgcggttcg
     5161 gcgtttggtc gcacgttaca accacaactt agccaggcga gttttgaaaa cctgctggct
     5221 gccgaaatgc taaaaaccga cgcccgtcag gatttgcgcc tgtgggtgct ggaagatgaa
     5281 agccggatga tcggttcgaa tcacctgccg gaatgtctgc gcgagcgaat gactcaggcg
     5341 gcgattgcgg tggtagaaga tccgtttgag atccgtcttg aacgcctgaa tgaagagtat
     5401 ttcttgcgta tgcatcatga ttttacccac gcttatggcg acgaacaggg ctggcaggaa
     5461 tattgcgaat acctgcatca cggactttcg gcgattaagc gtcggctggg gttacagcgc
     5521 tataacgaac tcgctgccca gctggatacg gcgctgacaa cgcaactcac caccggcagc
     5581 accgacggtc atctggcctg gctggtgccg ttacttaaag aatattacga cccaatgtat
     5641 cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc gcggtgagtg ggcggaagtg
     5701 gcggtatggg ttaaggcgca gtgatgggcg gtattctgaa acggcgattg tcgtgagaac
     5761 aaaatggttg caccagaata cagatataaa aaaaccgaca tgacagtcgg tttttttatt
     5821 ttttccgccc aagacttggt ggttccgggc agaaattaaa cagtcctcat ctagcaagtg
     5881 aattataagt caggatgcct acatattcaa acagctatgt tggcgataca atcccgacgc
     5941 tagtagtgat actgcctcaa gggattaccg cagtgcggaa aagtactttt aagtcctggt
     6001 caggatacag gtggtgctga ttctggtgct ctaatttgga gagagacagt cctcatctag
     6061 cacatgaaga aaatcatagt tataggcaac gtaagggtaa gctgtgattg tctttctccc
     6121 ttaagggagc aacggatgaa aacttttctc gataaagttc tcatcgttct taaggcgcta
     6181 atcgcgctgc tggaactgat ccgtcagttt attgaataac tgaggaaacg tagctaaggc
     6241 cgaaggttta cctccttcgg cctttaaaaa aaccgacatg acagttggtt tcatatttat
     6301 tgccaaaatg cacactgttc atgccagatg cggcgtaaac gccttatccg gcctacacaa
     6361 tcttgcaaaa tcaatatatt gcacgctctt cgtaggcctg ataagcgtag cgcatcaggc
     6421 aatctaacac ctgtcatcac cccacaaact gcctgaacag cgccttaccc ttaaccagtc
     6481 gcgcacccag ccagccgccg aaaagcgaca gcagcagcgc accgctgcac ggcagaacaa
     6541 tccacaatcg ccagtctggc tcccacggga aatcaaacac tttcgcctgc aacactgcca
     6601 gtgccgtttc cgcaccaatt gcggccacca ggccggaaac aaacccaagc atggcgaaca
     6661 tcacaccaca atgtcgtgcg cagcagtttt ttccccgcac cgagtgtgcg ccataccacc
     6721 agctcctgat gacgctggcg cattcccacc tgcacctgtg ccagcaacag caacataccg
     6781 caggcggtga ccagcaccac catcacctcc agcgcccgac ttacctgctc cagcacctga
     6841 ccgacctgtt ttaaaatcgc gccaatgtct aacaggctaa tggtcgggaa ctggcggttg
     6901 agttgcgtca acatgccgtt gccattctcc cagcggaaac tggtaagcca gctctgcggt
     6961 tgcccgtcta atgccccttc agggaaaata aaatagaaat taggccgcag actttcccag
     7021 tccactttgc gcaggctggt cactttagcg cggaactcct gggtatcgcc cataaaagtc
     7081 acggtatcac cgagggcaac gtttaagcgt ttcgccagcc cctcttccat cgacacttca
     7141 tcggcttttg gcggccagtt accggcgaca atcggattat gatcgggccg cgtattttgc
     7201 caggtaagat tgagttcgcg attaagcgcc tcatcttcat taccttctgt cggcttatca
     7261 ttaatcgccg tcagccgcgc ccgcactacc ggataaaatg attccgggac tatatgatgt
     7321 tccgcgagga acgcttttag cggtgctacc tgttctgtgg cgatgttaat taaaaagtag
     7381 ttcgggcttt ctggcggtag ctgctgctgc cagcggtcga gcagatcgcc acgcagcacc
     7441 agcagcagtg ccagcagcat aaaggagagc gaaaatgccg aaagctggct taacgttgac
     7501 cacggctgac gtaacaggcg gctaaccgcc aggcgcagag gcagcgattt cagcgtcatg
     7561 cggcgaagta cattcagcag catccagccc agcacaccgc acagcaaagc cagtactacc
     7621 gcgcccgcca gcaccgccca aagcagcatg ctgccaccca ttaatccggc gagcagcagc
     7681 acaaccacca cactgacaat cggcagataa aacttcagcg gccagacgtt cgcgaccaca
     7741 tcattacgta atacgcgtaa aggctgcgtt gccagcaaca agcggtatgg tcgtagcccc
     7801 accagcagcg agatgaccgt catggtgcca agcgcccaca gccacggcca gaggctggct
     7861 ggcggtagtg cagcaggcag aacgggcttg agcagcacca tcaacacgtt ttcgaacaac
     7921 aggcctatgg ccccaccggt aacggctgaa agcgtcagca ccatcaacca ctgaccgacg
     7981 attagcttac gcagttgcgc tcgccctgcc cccagcgttt tgaggatcgc caccagatcg
     8041 tagcgactgc gacagtaatg attcatcgct accgccaccg ctgccactgc cagcagcaag
     8101 gtcagaagcg ccgaaagcag caggaactgt tgcgagcgtt ccatcgatcg ccccagcgcg
     8161 ccttcgtcct gttccagacc gtaccagcgt tgttcgggtt taagctgagg taacaaccat
     8221 ttctcatagc cgtcgagctg gttctcgttg ccgccgaatt tataacgcca ggtgacccga
     8281 ctccccggct gcacggctcc ggttttatcg acatccgcca gattcatcat cagacgcgga
     8341 gccatctgga aggggttaaa accggaatcc ggttcctgaa tcacttctcc ggcaatccgc
     8401 aaggtggcat cgcccacgtc aatggtgtcg cccgttttca ggttaagcag tgccatcagg
     8461 cgtggggcca gcaatacgct gcccgcctgc ggtttcaggc cagggggatt agtttgcaga
     8521 ccgccataca tcgggtagat atcatccacc gctttgacgt tcgccagctg cggtgtgtcg
     8581 cctgcaaagg tcattgtggc gaaagtcagc tgcttgccga ctttcaggcc gcgcttttgc
     8641 gcttcctcca gccacgcttg cggcacttcg cgtgaactgc gcaacgcccg atcgcccgcc
     8701 ataaactcac ggctttgctg gcttaagccc ttctccatgc gatcgctgat attgcccagc
     8761 gccagcacgc aggccaccgc caggcttagc gccagccaga caattaatag cgacggcgaa
     8821 cgccattcgc gccagaacca acgtgcaatc atgcttcctc ctgcaactgc ccgttcacca
     8881 gccgtaagca gcggtcgcag cgtgccgcca gttgcaggtc gtgggtcacc ataatcaacg
     8941 tggtgccatg ttcacggttg agggaaaaca gcaggtcggc aattttatcg cccgtctggc
     9001 ggtcaaggtt gccggtgggt tcgtcggcaa acagcacatc aggtcgacca ttaaaggctc
     9061 gtgccagcgc cactcgttgc tgttcaccgc cggaaagctg tgccggaaga tgatccagac
     9121 gtttacccag ccctaactgt tcgagcaacg ctttcgcccc gttacgactt tccgcgctac
     9181 tctcaccgcg cagcagagcc ggaagctcga cgttttccag cgcgttaagg gtaggaatta
     9241 acataaatga ctgaaaaaca aagccgacgt gcttcgcgcg caactttgcc cgcgcttctt
     9301 cgtccatatt atgtagcggt tgtcccacca gactcacttc gccactgctg ccgtcatcaa
     9361 gcccggcgag gatcgccagc aaggttgact tacccgatcc cgactcgccc accagtgcga
     9421 tggtctcgcc acgtttgaca accagctcaa ctccggtgag gatggagagt tcatgctccc
     9481 cctgaccgac ggacttctta agatgatgaa cttcaacaat gttttccgct ggcatttgcc
     9541 cttcctgttc ctggtcctgt taaccttccg tgccgccgca gcggacacgt tattgattct
     9601 gggtgatagc ctgagcgccg ggtatcgaat gtctgccagc gcggcctggc ctgccttgtt
     9661 gaatgataag tggcagagta aaacgtcggt agttaatgcc agcatcagcg gcgacacctc
     9721 gcaacaaggg ctggcgcgcc ttccggctct gctcaaacag catcagccgc gttgggtgct
     9781 ggttgaactg ggcgcaatga tggtttgcgt ggttttcagc cacagcaaac cgagcaaacg
     9841 ctgcgccaga ttttgcagga tgtcaaagcc gccaacgctg aaccattgtt aatgcaaata
     9901 cgtctgcctg caaactatgg tcgccgttat aatgaagcct ttagcgccat ttaccccaaa
     9961 ctcgccaaag agtttgatgt tccgctgctg ccctttttta tggaagaggt ctacctcaag
    10021 ccacaatgga tgcaggatga cggtattcat cccaaccgcg acgcccagcc gtttattgcc
    10081 gactggatgg cgaagcagtt gcagccttta gtaaatcatg actcataaag caacggagat
    10141 cctgacaggt aaagttatgc aaaaatcggt cttaattacc ggatgttcca gtggaattgg
    10201 cctggaaagc gcgctcgaat taaaacgcca gggttttcat gtgctggcag gttgccgaaa
    10261 accgaatgat gttgagcgta tgaacaacat gggatttacc ggcgtgttga tcgatctgga
    10321 ttcaccagaa agtgttaatc gcgcagcaga cgaggtgatc gccctgaccg ataattgtct
    10381 gtatgggatc tttaacaatg ccggattcgg catgtatggc cccctttcca ccatcagccg
    10441 tgcgcagatg gaacagcagt tttccgccaa ctttttcggc gcacaccagc tcaccatgcg
    10501 cctgttaccc gcgatgttac cgcacggtga agggcgtatt gtgatgacat catcggtgat
    10561 gggattaatc tccacgccgg gtcgtggcgc ttacgcggcc agtaaatatg cgctggaggc
    10621 gtggtcagac gcgctgcgca tggagctacg ccacagcgga attaaagtca gcctgatcga
    10681 acccggtccc attcgtactc gcttcaccga caacgtcaac cagacgcaaa gtgataaacc
    10741 agtcgaaaat cccggcatcg ccgcccgctt tacgttggga ccggaagcgg tggtcgacaa
    10801 agtgcgccat gcttttatta gcgagaagcc gaagatgcgc tatccggtaa cgctggtgac
    10861 ctgggcagta atggtgctcg agcgcctgct gcctgggcgc gtgatggaca aaatattgca
    10921 ggggtgagtt gaagcgcgcg cttaagcccc catgtcaaag aaaatatcga caacagagag
    10981 cgactccatg tccgtagaaa atattgtcaa cattaacgaa tctaacctgc aacaggttct
    11041 tgaacagtcg atgaccactc cggtgctgtt ctatttttgg tctgaacgta gccagcactg
    11101 tttgcagtta accccgatcc tggaaagcct cgcggcgcag tacaacgggc aatttattct
    11161 ggcgaagctg gactgcgacg cggagcagat gattgccgcc cagtttggtc tgcgcgcgat
    11221 tccgaccgtg tatctgttcc agaacgggca accggtagat ggcttccagg ggccgcaacc
    11281 ggaagaggcg atccgcgccc tgctggataa agtgctgccg cgcgaagaag agctgaaagc
    11341 gcagcaggcg atgcaactga tgcaggaagg caattacacc gatgccctgc cattgctgaa
    11401 agacgcctgg cagttgtcga atcagaacgg ggagatcggc ctgctgctgg cagaaacgct
    11461 gattgcgctg aaccgttctg aagatgcgga agcggtgctg aaaaccattc cgttgcagga
    11521 tcaggacacc cgctaccagg ggctggtagc gcaaatcgaa ctgctgaagc aggcggctga
    11581 taagccggaa attcaacagt tgcaacagca ggtggcggag aatccagaag atgccgcact
    11641 ggcgacgcaa ctggcgctgc aactgcatca ggttgggcgc aatgaagagg cgctggagtt
    11701 gctgttcggg catctgcgta aagatctcac cgccgcagac ggtcagacgc gtaaaacgtt
    11761 ccaggagatc ctcgctgcgc tgggtacggg tgatgcactg gcgtcgaagt atcgccgcca
    11821 gctgtatgca ttgttgtatt gatattccgt tgttcggcac atgaaccaca acgccggatg
    11881 cgatgctgcg catccggcat cgcatcattt cttcttcaat tgagtcacca ccaactggtg
    11941 gcgcgaatta taaaacttac gataggttaa atagcaggca ataatggtcg acaagctggc
    12001 ggttgagagc agcataaagg tcaccataat ctgatattta atcgccttca ccggatcaat
    12061 cccggcaaat atcagcccgg acatcattcc tggtaaactc actaagccaa ccgtttttgc
    12121 tgaatcgacc gtcggaatta aagccgcgcg aatactgtcg cgaatcaata tcgctgaagc
    12181 ctgcttcggc gtcgcgccaa gactcagttt ctcctgaatc tgttgctgtt cgctaatgac
    12241 ccgttgccct aaattgttgt aacacaaccc caccgctacc atggcgttac cggcaatcat
    12301 cccggcgaca gggatcacct gcatcgggat aaattcaatc gcccctgaga gaatcagcac
    12361 cgccagggta attcccgccc cgaccgtaat ggcaataaac gatgagataa aagctttagc
    12421 aatatattta ctgcgttttt gcgcgttcca cgccgcatta aagcagataa ataacaccat
    12481 caataatgtc aggctggcat catccacgct gaaaatatac ttcagcacat agccgacaat
    12541 aatcagctga attatcgctc gcccgacgct ccatagaata tctttctcca gcgccagttt
    12601 ttctttatgg ctaattaaga ttgccaccac caccagcatt aatgccagtg ctaatgattc
    12661 gttagtaata ttatgcgagt tcatagcgtg cttcctgcat ttctccggca tgcggttgca
    12721 gtgtaatcac tttatccgca tgattaattt cgtctttatc gtgtgtcacc cacagcacgg
    12781 caatattttg ctcgcgcaca taacgatgga tcatctcatt gacgttatgt ttattacttt
    12841 catccagcgc actggttatt tcatccagca ataaaacctt cggcataaat tgcaggttac
    12901 gaatcaatga gatgcgttgt ttttcaccac cagatagctc ggcgatattc ttcgtcaaaa
    12961 tgctgtccgg caaagcgaag cgttcgagaa aatcgagaaa aatggccggg tcaggctgct
    13021 ggtcacggat ctgccaggga aagatcagat tatcgtatac cgtatcgcca aacagcgtcg
    13081 gcgtctgggc gcagtaagag acttgctggc ggtagatttc tggttttagt gtgctgacat
    13141 cctcaccttc aaacagtaac gttccgctgg ttgggctgat caatgaagca actattttta
    13201 gcagcgtact tttgccacaa ccagaaggac cggtaattaa cttaaattcg ccagcacgca
    13261 gcgaaaaatt gatgttatta agaatcttcg tatcacccgc cagatatcct acgttttgta
    13321 gctgaagcaa aggactattt tcctgcatcg ctgttccctt tttctgattt ttactaaaaa
    13381 cagtttatcc ttcgcaggaa taagggggaa ctctctttca gtaatcaggt ataatttgtt
    13441 taatcaagca gcaattttgc tgattatgga cggtacaaca ggaggttttt ccgatgctta
    13501 tctttatccc gattctcatt tttgtcgcgc tggtcattgt cggcgcgggc gtcaaaatcg
    13561 tgccgcaggg ctatcaatgg acggtagaac gttttggtcg ctataccaaa acgttacagc
    13621 cggggctcag tctggtggtg ccgtttatgg atcgcattgg tcgcaagatc aatatgatgg
    13681 agcaagtgct cgatatccct tcccaggaag ttatctcgaa agataacgcc aacgttacca
    13741 tcgacgcagt ctgttttatt caggtgattg acgcgccacg cgctgcctat gaagtcagca
    13801 atctggagct ggcgatcatc aacctgacca tgactaacat ccgtaccgtg ttgggttcaa
    13861 tggaacttga cgaaatgctc tctcagcgcg acagcatcaa ctcacgcctg ctgcgtattg
    13921 tcgatgaagc caccaacccg tgggggatta aagtcacccg tattgaaatt cgcgacgtgc
    13981 gcccaccggc agagcttatc tcttcaatga acgcgcagat gaaagcggaa cgtaccaaac
    14041 gcgcttacat tcttgaagcg gaagggatcc gtcaggcgga aatcctcaaa gccgaaggtg
    14101 aaaaacagtc gcaaatcctg aaagcggaag gcgaacgtca gtcggcgttt ttacaggctg
    14161 aagcgcgtga acgttccgcc gaagcagaag cccgcgccac caaaatggtg tctgaagcta
    14221 tcgcctccgg tgatattcag gcggtgaact acttcgtagc gcaaaaatac accgaagcgt
    14281 tacagcagat cggttcctcc agtaacagca aagtagtgat gatgccatta gaggccagca
    14341 gcctgatggg gtcgattgcc gggattgccg agctggtgaa agacagcgcc aacaaacgga
    14401 ctcagccatg atggagttaa tggtcgttca tccacatatt ttctggctca gtctcggcgg
    14461 gttgctgctg gcagccgaga tgctgggcgg aaatggttat ttgttgtgga gcggcgtggc
    14521 agcggtgatt accggcctgg tggtctggct ggtgccgctg ggttgggagt ggcaaggggt
    14581 aatgtttgcc atcctgacgc tgctcgccgc ctggctgtgg tggaaatggt tgtcgcggcg
    14641 ggtgcgcgaa caaaagcaca gcgatagtca tttaaaccag cgcgggcagc agctgattgg
    14701 ccgacgtttt gtgctggaat ctccgctggt caacgggcgc ggtcatatgc gcgtcggtga
    14761 cagttcatgg cctgtcagcg ccagcgagga tctcggtgca ggtacgcatg ttgaagtcat
    14821 tgcgatagaa gggataaccc tacatatccg agccgtctcg tcttaatcac cctgcccgat
    14881 gatgacagca gccggagaga ttttcgataa tcgggcagtc ggcgctgtca tcgccagggc
    14941 aggcattcgc cagtgccagc agctggtcgc gcatggattg cagctcctca atgtgtcgtt
    15001 cgatctccgc caccttctcc agcgtgcgcc gtttgacgtc ggcgctgtgc cgctgcgggt
    15061 cgttaaacag attcaccagc tcgccgctct cttccaggtt aaagcccacc tgccgtgcct
    15121 ggcgcagtaa ggtcagttcg ttgagatgct gctgcgtgta ggtgcgataa ccgttttcgc
    15181 tgcgcatcgg cggcgtcacc agccccttct cttcatagaa gcgaatggct ttgctggtca
    15241 ggccggtaat ttttgctaca tcgctgatgt tcatcgttcg cgcaacgcct cctgtgcttt
    15301 gttaaagggt ttcaacagat attcaagcac gtttttttgc cagtgcgaat atcgaccgcc
    15361 accacaataa tacaggaagt actgcgcaca gagaggtgtt atacggtttt attacgcttc
    15421 atgaccattg ccacaataag gctgagtaag attgcaccaa taatccatat caacgcacgg
    15481 ctgccctggg accacaggct gtagataaag ccaacgatca ccaacaacat caccaacgta
    15541 ccaacgataa gaatcggcaa cgaggcgtga atatcatgac gcaggcggat cgccaccaca
    15601 aacaccgcca gatagcagat caaaaaggtg gcgctggcaa cgctggcgag tgagtctaaa
    15661 tttagcgccg ccgtcatcag cattatcaac accacgacga tgatattgcc ccaggtactc
    15721 tgccgccaca gggatttatt cattagcttc ggcagttcgc gttcgctgcc catgttgtcc
    15781 atgatgttaa acacggcgaa caggttcgcg ttaatgcctg aagccgtcgc cagtaaagcg
    15841 ccgatgacga cgatcacata acccacatgc ccgagcagcg gagaagcagc ctgcgctacg
    15901 gcggtatcag catatttttc taactctaat gccgatacat cgctaagcaa aaccagcgcc
    15961 agcgagatat aaagcaacgt ggtaacgcca atcgccacca gaaacgcccg tggcataatg
    16021 acctgcggat ctttcacttt atccgccgcg ttcgccatca tgccaaagcc cgcataggca
    16081 aggaaggtta tcccaataca ggagaagaac gcaccggagc tggggggcgc agagacggaa
    16141 atatgcgccg gttgcagcga ccagacaccg gcaataatca gcaataacag gatcatcatt
    16201 ttaatgccga cgaggatcac ttccagccgc cctaccgcat ggttgcttaa ggagttgaaa
    16261 agcgtcatca ccgcaatgat ccccaacgcg tagagcaaaa taaggtgctc ctcctggctg
    16321 ccttcatgca aaaactgcac ggcataagcg ccaaaagcac gggcaaccat ggcgatgctc
    16381 accgccagcg tcaacaggta tagcaacgag agcgctagcg aaaagacgcc gttgcctaat
    16441 ccgcgacgaa aaaagtcgat aataccgcca ttgctgggat agctcgcccc cagacgcgca
    16501 taggcataac cggaaaacat agccacaata ccgccaaaag caaaggcgcc ccaggtcgag
    16561 gcttccatta gcaacgcagc ctgccccagc agcgcgaaga tccccgcccc caccattgcc
    16621 ccaataccga tggaaacgac gttccataga ccgaggggtt tgttaccgtt attaccttcc
    16681 gtgttcatca tgatcagccc ttaaacacgt tatagccgag ttgcttagcg accgatgcca
    16741 ccattttttg accgcgaaca ctattgcctt cttcgtccag cggtggtgag aacgcggcaa
    16801 ttcccatcac gccagggacg accgccagaa taccgccacc tacaccgctt ttgcccggta
    16861 aaccaacgcg atacgcccag tcaccggagc gaccatacag cccttccatc atcatttcgg
    16921 ccagaatgta cggcacgttg tcggcctgaa gaacgcgttt atgcgtcaac ggattcacac
    16981 cacccgccgc cagcgtcgcg ccaagcgttg ccagttcaac agtattgatg agcgtagagc
    17041 actgacgggt gtacacgtca caggcttcca ttgcatcaca atagagatat ccggcggagt
    17101 acagcagcca ggcaatggcc cgattatgga agttggttgt ttgttccgac tggttgactt
    17161 cgtcagagag cgctacctgc tcaccagcca gttgctgttg gatatgtaaa attcgctgcc
    17221 agcgttgttc ggcgttttca gcgttaatca ggctggtggt ggcaatagcg ccagcattta
    17281 ccagtggcga aagcggtttg ccgccatgca actctaaggc gataactgag ttaaagggca
    17341 atccggtcgg gtcagcgcca attttgtcct gtaccgcctg cgggccgaca tcttctaacg
    17401 caagggctaa cgtacagact ttagagatgg attccagtgc aaagcggtaa tcactgtcac
    17461 ccgcactata gacgttgcca tcgcaggtca cgatagccac tgccgccagt tgacctggta
    17521 cattcgccag aaagggaatg taatcggcat tttgtccgcc gttaagtgag tgaaattggg
    17581 tgtaagcctg atccactgcc tgctgtaatt tgtttgcatc taacatcttt tgttaactcc
    17641 tttttataga tgcgggaggt aattcctcac cccagtgccg attttcaggc atcctgattt
    17701 aacttagcac ccgcaactta actacaggaa aacaaagaaa taaatgtcta atcctgatgc
    17761 aaatcgagcc gattttttaa tctttacgga cttttacccg cctggtttat taatttcttg
    17821 accttcccct tgctggaagg tttaaccttt atcacagcca gtcaaaactg tcttaaagga
    17881 gtgttttatg tcacaaacta tcgacctgaa cctggacggc ctgtcctgcg gtcactgcgt
    17941 taaacgcgtg aaagaaagtc ttgaacagcg cccggatgtg gaacaagcgg atgtgtctat
    18001 cactgaagcg cacgttaccg ggactgccag tgcagaacag ctgatcgaaa ccatcaaaca
    18061 agcgggttat gacgcatctg taagccaccc aaaggctaaa ccgctggcgg agtcatcaat
    18121 cccgtcggaa gcactgacag cggtttctga ggcgcttccg gcagcgaccg ccgatgacga
    18181 tgacagccag cagttgctgc tgagcggcat gagctgcgcc agctgtgtca cccgcgtaca
    18241 aaatgcgctg caaagcgtac cgggcgtcac tcaggcacgg gtaaacctgg cggagcgtac
    18301 tgcgctggtg atgggcagtg cctccccaca agatttagtg caggcggtgg aaaaagcggg
    18361 ctacggcgcg gaagcgattg aagatgacgc gaaacgccgc gaacgccagc aagaaaccgc
    18421 cgtcgctacg atgaagcgct tccgctggca ggcaattgtc gcactggcgg tgggtatccc
    18481 ggtgatggtc tgggggatga tcggcgataa catgatagtc accgccgaca accgcagcct
    18541 gtggttggtt atcggcctga taaccctggc ggtgatggtt ttcgccagcg gccattttta
    18601 ccgcagtgca tggaaaagcc tgctgaacgg tgcagcgacg atggatacgc tggtggcgct
    18661 gggaacgggc gtggcgtggc tctattcgat gagcgtcaac ctgtggccgc agtggttccc
    18721 gatggaagcg cgacatcttt actatgaagc cagtgcgatg attatcggtc tgatcaatct
    18781 cggccatatg ctggaagcac gcgcacgtca gcgttcttct aaggcactgg agaaattact
    18841 cgatttaacc ccaccgacgg cgcgcctggt tactgacgaa ggtgaaaaaa gcgtgcctct
    18901 ggcagaagtg cagccaggta tgttgctgcg cctgacgacc ggcgatcgcg tgccggtaga
    18961 tggcgagatt acccagggcg aagcatggct ggatgaagcg atgctgacgg gcgaaccaat
    19021 cccgcagcaa aaaggcgaag gcgatagcgt ccatgccggg acagtggtac aggacggcag
    19081 tgtgctgttt cgtgccagtg cggttggcag ccataccacg ctatcaagga tcattcgcat
    19141 ggtgcgccag gcccagagca gcaagccaga aatcggtcag ctggcggata aaatctcagc
    19201 catatttgtg ccggtagtag tggttattgc gcttgtcagt gcggcaatct ggtatttctt
    19261 tggtccggca ccgcagattg tctataccct ggtgattgcc actacggtgc tgattatcgc
    19321 ctgcccgtgt gcgctgggac tggcgacgcc gatgtcgatt atttccggcg tcgggcgggc
    19381 ggctgagttt ggcgtgctgg tgcgggacgc cgacgcgctg caacgcgcca gtacactcga
    19441 taccgtcgtg ttcgataaaa ccgggacgct gaccgaaggg aagccgcagg ttgtcgcagt
    19501 gaaaacattt gctgatgttg acgaagcgca ggcgctgcgt ctggcggcgg cgctggagca
    19561 aggttccagc catccattgg cacgagcgat ccttgataaa gcaggcgata tgcagctacc
    19621 gcaggtcaac ggtttccgca cattgcgcgg gctgggcgtg agcggtgaag cagaaggtca
    19681 tgcgttattg ctgggtaatc aggcgttgtt aaatgatcag caagtcgata ctaaagcgat
    19741 tgaagccgat atttcagctc aggcatcgca aggagcaacg cctgtgctgc tggcgattga
    19801 cgggaaagca gtagccctgc tggctgtacg cgatccgttg cgtagtgata gcgtggcggc
    19861 gctgcaacgc ctgcataaag cgggatatcg tctggtgatg ttgaccgggg ataacccaac
    19921 caccgccaat gcgatcgcca aagaagctgg aattgatgaa gtgattgccg gagtgctgcc
    19981 ggacggtaaa gccgaagcga tcaaacgtct gcaaagtgaa ggacgtcagg tggcaatggt
    20041 gggcgacggc attaacgacg cgccagcgct ggcccaggcg gatgtcggca ttgcgatggg
    20101 tggcggcagt gatgttgcca ttgaaaccgc ggcgattacc ctgatgcgcc atagcctgat
    20161 gggcgttgcg gatgcgctcg cgatttcccg cgcaacgctg cgcaacatga agcagaacct
    20221 gctcggtgcc tttatctaca acagcatcgg tattccggtc gccgccggta ttttgtggcc
    20281 gtttactgga acactgctta acccggtagt tgccggagcg gcaatggcgc tctcgtcaat
    20341 taccgtagta agtaacgcca accggttgct gcggtttaaa ccgaaggaat aagtacgtca
    20401 ttgcggatgc gctgctttag ggcggcgcat ttggcatcga cgcatgactg ccggatgcgg
    20461 cgtaaacgcc ttatccggcc tacggttcag gcacaggttt gtaggcctga taagacgcgt
    20521 aagcgtcgca tcaggcaatg gtgctcggat gccggatgcg gcgtgaacgc cttatccggc
    20581 ctacgagatg aagcgatgtt ttggccttac gcgacctttt ttgcacgctc ttcacggcgt
    20641 gccaaatatt cagccactgt ttcaatccgt cccaattctt cctgggtgcg catgttgttt
    20701 tcaacttccc aaagatcaac tgccgcctgg aggtttagcc taaaatcgac tgtggtatca
    20761 aaaactttcg ccagacggaa tgccatctca gtggtgagtt tacgattgtt attgatcagt
    20821 gcactgacgc gattacgatg aacatgcagc aactctgcta actcattgat tttcaaatcg
    20881 agcggttcca gatattcata gagaagaata tctccgggtg tcgtcggttt tcttgttgcc
    20941 tgtttcatgg tttttaactg ttgcttccct gtgaatagcg atgcaagaac atactgaatc
    21001 atacaaacac attaaacaag tccccaactc catttgcgcc atccctcacc gcgcgctagc
    21061 atgatatttc acaaagggag gtcgtatgga tctgttgtac cgggtaaaaa cgctttgggc
    21121 cgcgctgcgc ggtaatcatt acacctggcc tgccatcgat atcaccctcc ccggcaatcg
    21181 ccattttcat ctgattggca gtattcatat gggtagccac gatatggctc ccctgcccac
    21241 ccgtttgctc aaaaagctca aaaacgccga tgcgctgatc gtcgaggcgg atgtttccac
    21301 cagcgatacg ccttttgcta atttgcctgc ctgcgaggcg ctggaagagc gcattagcga
    21361 ggagcaactc caaaacctgc agcacattag ccaggagatg ggcatttctc cctcactctt
    21421 ttctacccaa ccgctgtggc aaatcgcgat ggttcttcag gcgacgcagg cacaaaaact
    21481 ggggctgcgg gcagaatacg gtatcgatta ccagctattg caggcggcga agcaacaaca
    21541 taaacccgtg attgaactgg aaggggctga aaaccagatt gccatgttgc tccagctccc
    21601 tgacaaagga ctggcgctgc tggacgatac gctgacccac tggcatacca acgcacggtt
    21661 gctgcaacaa atgatgagct ggtggctgaa tgcaccgccg caaaataatg atataacgct
    21721 gcccaatacg ttcagtcagt cgctgtacga tgtgctgatg catcagcgaa atctcgcctg
    21781 gcgggataaa ttacgcgcca tgccgccggg gcgatatgtg gtcgcggtcg gtgcactaca
    21841 cctgtatgga gaagggaatt tgccgcaaat gttgcgctaa aaaaatggcc aatatcgcta
    21901 ttggcccgtc aaagaggaat ttcatttttt tattattatg ccgtcacttt aagcgacggt
    21961 gtaactcgat tgtcgctcaa tctcaccatc ctgccaatac tcttaggcaa gatgactctt
    22021 ttttttggac cgccatcagg cgtatgcttg cttcgttttt ttgtttaagg caggaaaatt
    22081 accatgacac ccgcagttaa attactcgaa aaaaacaaga tttcgtttca aatccatacc
    22141 tacgagcacg atccggctga aaccaatttt ggcgatgaag tcgtcaaaaa attaggtttg
    22201 aatccggatc aggtctacaa aacgctgctg gtggcagtga acggtgatat gaaacacctt
    22261 gccgtggccg ttacgccggt cgcaggtcaa ctggatctta aaaaagtagc aaaagcgctg
    22321 ggtgccaaga aagttgagat ggccgatccg atggtcgcgc agcgttcgac gggatacctg
    22381 gttgggggga ttagcccact ggggcaaaaa aaacgtctgc caacgattat cgacgccccc
    22441 gcacaagaat ttgccactat ttatgtttcc ggcggcaagc gcggactgga tatcgaactg
    22501 gcggcaggcg atctggcaaa gatcctcgat gccaaatttg ctgatatcgc ccgccgcgat
    22561 taagcattgt gccggatgca aacatccggc actttcggat tactgccagc tcacctcacc
    22621 tttcggttca taaacactca catccagcgg cgagcttttc tggatatacg ctttcagcac
    22681 ttcggcatca ataaagccgg tattcacata gcccggtttg ttatcaaggc gcggatatcc
    22741 atcaccgccg gtggcattga agtttaatgt cgccatacgg taagttttcg ccggatcgac
    22801 cggttcgcct ttgattttaa ggtcgttcag tttgccgtct ttcgccacaa agctaacgtt
    22861 ggcaaattgc gggtaggcac ctgaatctgg cttcatctgc gcgacggcgg tcaggtaatc
    22921 aatcacctct ttaccggtca tgtcggcata caccaccaca ttgccgaatg gctgcacttt
    22981 cagcacgttt ttatagctga tatcgcctgc ttcgatagaa tcacgaattc cgcctccgct
    23041 catcaccgca aagtcggcac cagtgcgatc catttgggct gccagaatca accgccccat
    23101 attggtctgt acaaaacgca ctttgtcacg atcgccttcc agacgaccat tggttttgcc
    23161 tattttcact tccagctgcg ctttgccttt gttctggaac ggtgataaca gcgagatcat
    23221 ttgctggttt tcagcgattt caggagtgta aagcacgcgc tcgcttttcc cgtcttccca
    23281 ggtcactttc ttcttcaggt tcaccggaat cagctggtag ttaaccattt tcatttcgcc
    23341 attacgaaac tcaaaatcag cccgtcccac gtatttgccc cactcatgcg cctgcacaat
    23401 ccagatgccg ttttgttgat ctggtttgca tggcgtaccc ggcacgtaat cgacctgttt
    23461 tttgttttct gccgccatgc agaccggatc ttgcgagtga ccaccgacga tcatcgccag
    23521 cgatccggca ggcagcgcgc gtgccatctc cacatcgccc ggtgcgttag agccgtgctc
    23581 accattatcg taatgcccca tatgggtcgc cgcgataata atgtctggct tttctgtctg
    23641 ttgcagctcc tgaatcacca gcttcgcttc atcggcgggc ttacgaaatt cgatatcagt
    23701 gaagtattcc aggttaccaa tttttgctgt gtcatcggtt gtcagcccaa taacggcaat
    23761 tttcagatcc tgacgcttaa acagcgccca cggtttaaac aggcgctcgc cagtactttt
    23821 ctggtagata ttcgcggaaa gcaacgggaa cttggcccac ttttcctgct ggcgtaatac
    23881 ggtgagcgga ttatcaaatt catgattacc gatcgccatc gcgtcatagc ccaccagatt
    23941 cataccgcga aaatcaggtt cggcatcctg taagtcagac tcgggcacgc cagtgttaat
    24001 gtcgccaccg gaaagtagca gcacgctacc gccttcagcc gcaacctctt tgcggatacc
    24061 atccaccagc gttttttgcg ccgccagacc atattcgcca tattcattgc gccaaaaatg
    24121 cccatgatga tcattggtat gcagaactgt aattttgtag gttttatcct gctcatacgc
    24181 cagagcagtt tcactcgcca gtgtaaatgt ggttaacagc gctaacgcca cgccccgctg
    24241 caataatttc atacttctct ccctgacctg atttcaacgt gttgaaaaat agtatgacgc
    24301 aacatcatag acaagtctgc gtccggtgaa gataaatctg catccagatt agaaattctg
    24361 tattcggcgt tgagacttcc ttcagacgac gcatacaggt atgttagtcg ggtaataatc
    24421 acaaataaga tcattcttta catcgcaacc ctgaaaaagt gactttccta tggcaatgag
    24481 tgaacaaacc cagcctgtgg cgggcgcggc tgcgtcaacg accaaggccc gaacatcgtt
    24541 tggtatttta ggtgctatca gcctctcaca tctgctgaac gacatgatcc aatcgctgat
    24601 tctggcgatt tatccgctgc ttcagtcaga attttctctg acatttatgc agattggcat
    24661 gataaccctc accttccagc tcgcctcttc gctactgcaa ccagtggtcg gctactggac
    24721 cgataaatat ccgatgccgt ggtcgttgcc aattggcatg tgctttacct taagtggtct
    24781 ggtgctgctt gcgctggcgg gcagttttgg cgcagttctg ctggcagcgg cgctggtcgg
    24841 taccggttca tcggtctttc atccggaatc ttctcgcgta gcccgtatgg cttccggcgg
    24901 gcggcatggc ctggcgcaat ctatctttca ggtcggcggc aactttggca gttccctggg
    24961 acccttgctg gcggcggtga ttatcgcgcc ttatggcaaa ggcaacgttg cctggtttgt
    25021 gcttgcggca ctgctggcga tcgtggtgtt ggcgcaaatt agccgttggt actcggcaca
    25081 gcaccgaatg aataaaggaa aacccaaagc gacgataatc aatccactgc cgcgcaacaa
    25141 agtggtactg gcagtcagca ttctgttaat cctcattttc tcgaaatatt tctatatggc
    25201 gagcatcagc agctattaca ccttttatct gatgcaaaaa ttcggattat ctatccagaa
    25261 tgcccagctt catctgtttg ccttcctgtt tgccgttgcg gcaggtacgg tgatcggcgg
    25321 gcctgtaggg gataaaattg gacggaaata tgtgatttgg ggctctatcc tcggcgttgc
    25381 gccgtttacg ctgattttac cctacgcccg cctgcactgg acgggggttt taacggtgat
    25441 tattggattt atcctcgctt cggcattctc tgccattctg gtctacgctc aggagctact
    25501 tccgggacgt atcggtatgg tttctggact ctttttcggt tttgctttcg gcatgggagg
    25561 tctgggagcg gcagttctgg ggcttatcgc cgatcacacc agcatcgagt tagtctataa
    25621 aatctgtgct ttcctgccac tattggggat gttgaccata ttcctgcctg ataaccggca
    25681 taaagactga tttcattgca gccaaaggca aactttggct gcatcgtttt acagtcgcca
    25741 taagcctttc ctctgttaaa ccgccttctg ttttccgaac cgtgcttttt ttgcagctaa
    25801 ttccattttt ctgcaaaatt caacaattat tcataaacat catcaatgaa attgtcataa
    25861 actattacct gtaattttgg ttttcccagc caaaaatgga caataaccac caggaaaagg
    25921 agacggaatg catcacgcca ccccgcttat caccaccatt gttggcggcc ttgtgctcgc
    25981 ctttatcctc ggcatgctgg ccaataaact acgtatttct cctctggtgg gatatctgtt
    26041 agcgggtgtg ctggcaggac cattcactcc gggctttgtt gccgatacca agcttgcgcc
    26101 ggaactggct gaactgggcg ttattttgct gatgttcggc gtcggtttgc atttttcgct
    26161 caaggatttg atggcggtaa agtccatcgc cattcccggc gcgatcgccc agatagccgt
    26221 ggcgacgctg ctgggtatgg cgctctccgc cgtgctgggc tggtcgttaa tgaccggtat
    26281 cgtgttcggc ttatgtcttt ccaccgccag taccgtggtg ttactgcgcg cacttgaaga
    26341 acggcaatta atagacagtc agcgtgggca aatcgccatc ggttggttga ttgtggaaga
    26401 cctggtgatg gttctgacgc tggtgttgct gcccgcagtg gcaggaatga tggaacaggg
    26461 cgatgtgggc tttgccactc ttgcagtcga tatggggatc accatcggca aggtgatcgc
    26521 atttatcgcc attatgatgc tggtaggtcg ccgtctggtg ccgtggatta tggcacgcag
    26581 cgcggcaacc ggttctcgcg agctgtttac cctgtcagtg ctggcgctgg cgttagggat
    26641 tgcctttggt gcggtagagc tgtttgatgt ctcctttgca ctcggtgcgt tctttgccgg
    26701 gatggtactg aacgagtctg aattgagtca ccgtgccgcc cacgatacgt tgccattgcg
    26761 cgacgcgttt gcggtgctgt tttttgtctc agtcgggatg ttgtttgatc cgttaattct
    26821 gattcagcaa ccgctggcag tgctggcgac gctggcaatt attctgtttg gtaaatcggt
    26881 cgcggccctg ttccttgtcc gtctgtttgg tcactcccaa cgtacggcat taaccatcgc
    26941 cgccagcctg gcgcagattg gtgagttcgc gtttatcctg gcgggactgg gaatggcatt
    27001 gaatttactg ccgcaggccg gacaaaacct ggtactggca ggggcgatcc tgtcgattat
    27061 gctcaacccg gtactgttcg cactactgga gaaatatctg gcgaagaccg aaacgctgga
    27121 agagcagacg ctggaagagg caatcgaaga agagaaacag atcccagtgg atatttgcaa
    27181 ccatgcgcta ttggtgggtt acggtcgtgt aggcagcctg ctgggggaga aattgctcgc
    27241 ctctgatatt ccgctggtgg tgattgagac gtcacgaacc cgtgttgatg agctgcgaga
    27301 gcgtggggtc cgcgctgtat tgggcaatgc agcgaacgaa gaaattatgc aactggcaca
    27361 tctggaatgt gcaaaatggc tgatcctgac gatccccaac ggttatgaag cgggtgagat
    27421 tgtggcgtct gcccgcgcga aaaacccgga tattgagatt atcgcccgcg cccattatga
    27481 cgatgaagtg acgtatatca ccgaacgtgg cgcgaatcag gtagtgatgg gtgaacgtga
    27541 aattgcccgt actatgctgg aactgctgga aacgccaccg gcgggtgaag tggtgacggg
    27601 gtaaacggtg gttgccggat gtaaaatatg aaaaatatgt aggcatgata agacgcgcca
    27661 gcgtcgcatc aggcatcgag agccaaatgc cagatgcggc gtgaacgcct tatctggcct
    27721 acgaaccgac gataacttaa cgatcccagt aagactcttc ctggctgtct tcacgctccg
    27781 gcaagccgcg cgttaaacga ggtgaatgct ggttcagtac ctgatagctc acacggttag
    27841 catatttaca cacctgcgct aacgatgaat aagttaacca ggtgaattta tgtttgctgg
    27901 agtttggtac gttgctacga tggtagctgt tggcggtaat gtcatgcagc aacgctgcca
    27961 atgcgccatc ccccgctcca ttagtgttca tgattttttc cggcccgccc atgtacggcg
    28021 caatgtgcga atagacacgc agcggattct ggcaatcctt gtggcgcatg gcgcggctaa
    28081 actcatactg gttgaattcc gctatagcgc ccggcagcag cggatgctgg gttttacgtt
    28141 tcgcttcgtc ttcggtaaag cccgccatat acaagccgat tggcccggcg gtgcacagca
    28201 ccagctctac ccagtccagc gccttgtcag atgccaacaa cggatcgctt tctccggtca
    28261 acgcttcggc ttcatcttcg ttcatcgcaa ggatagaaac gtggtctttg aggaattgct
    28321 gccaccactg cggattctcg gcaatgacaa acttggtgcc cagcgtcagc accaccggta
    28381 cgttatattt cttcgcgtac tcaatggctt tcatggttgc ttccggcatg ggttcacccg
    28441 gcttgcaacg caccagatat gaggtgagaa ccagtgccga ggctccggca atcacatctt
    28501 ccggaatgct ttcagcccgc agctggttca tgtggcctgg actgatagca aaggtacgtt
    28561 ccccggactc gccaatcagc gtaaagcaac gaccaatcgg accatccacg ccttgtagat
    28621 agttaagatc ggtacggctg gaagtgttac acaggtaacg ataggcataa ctgccaattt
    28681 caatattgct gcacatgacg cccagcagca ccgaacggtc gtccgcgagc accgagtagt
    28741 tgtgcatggt gttaccaatg gtgccacccg caaactgatg ggtaatcagg tttttctgtt
    28801 ttagctcctg ataaagcact tcggctacgt catcctcaat caccagtgaa tgcccggcgc
    28861 ttaatccata acgctcaatg aattcatcat ccactttcgc ttcaatatcg accagcgtct
    28921 ggtcaatacc tacgacccag gcggcgctgg tttcgttttc tggctggaat tgctgaagca
    28981 gcggatcgcg tgcatttaca gggaagtaat gtttggattt acgtttaccg ggaaatttca
    29041 tggttgtttt tacgggtggt taactgagcg gcgaatgtta acacaatctg acgggagcgc
    29101 gcgagacgca gccagtttca atatcagcag gcgggatcgc attttcatgg cagtggttcc
    29161 ttacaatgac gtaatttgaa aggagttttt gtatgaagcc ggaaaacaaa ctacctgttc
    29221 tggaccttat ttctgctgaa atgaagaccg ttgtgaatac tcttcagccg gatttaccgt
    29281 cctggcccgc aacgggaacg attgctgagc aacgacagta ttacacgctt gagcgccgat
    29341 tctggaatgc gggcgctcca gaaatggcaa ccagagctta catggttcca acaaaatatg
    29401 ggcaggtgga aacacgtctc ttttgtccgc agccagatag cccggcgacg ctattttatt
    29461 tgcatggagg cggttttatt ctcggcaatc tcgataccca cgatcgcatc atgcgcctgc
    29521 tggcaagcta cagccaatgt acggtgattg gtattaatta caccctttca cctgaagcgc
    29581 gttttccaca agcgatagag gaaattgtgg ctgcttgttg ttatttccac cagcaggcgg
    29641 aggattatca aatcaatatg tcccgcattg gctttgccgg tgattccgca ggtgccatgc
    29701 tggcgctcgc cagtgcgttg tggttgcgtg ataaacagat cgattgcggt aaaattgcgg
    29761 gtgttttgct gtggtatggg ctttacggat tacgggattc cgtgactcgt cgtctgttgg
    29821 gcggtgtctg ggatggctta acgcaacagg atttgcagat gtatgaagag gcgtatttaa
    29881 gcaacgacgc ggaccgcgag tcaccgtatt actgtctgtt taataatgat ctcactcgcg
    29941 aagttccgcc ctgttttatt gccggggcgg agttcgatcc gctgctggat gacagccgtc
    30001 tgctttacta gacgttagcg gcgcatcagc agccctgtga gttcaaactc tacccaggta
    30061 cgctgcatgc ctttttgcat tattcacgga tgatgaaaac cgccgacgag gctcttcgcg
    30121 acggcgctca gttctttacc gctcagcttt agcgatacgc ggcaacaaga ttagccatca
    30181 tttcgatatg ttccggcgtg gcattaagcg ctggaatata ttcatatttt ttcccgcccg
    30241 caccgaggaa gacctcacgg ttttgctcgg caatctcttc cagcgtctcc agacaatccg
    30301 cagcaaagcc cgggcacatc acctgtatat gacctacgcc tttttctccg agcattttca
    30361 gcgtttcgtc ggtataaggc atcagccagg gttcccggcc aaagcgcgac tgaaaggtca
    30421 tcatcacttt ttccggtacc atccccagtg cggaagccag ttcgcgagtc gttgtgcggc
    30481 aacgttgcgg gtaatcatcg ccttcatctg cataacgctg gggaatgcca tgataagaga
    30541 gcagcagcag atccggttcg ccatgtttgg caaaagaagc gcgtacgctg ttcgccagtg
    30601 cattaatgta atcgtggtta tcggcgtaat cacgaataaa cgatatcccc ggaatgctac
    30661 gtttgcgcgc cagaatgcgt gccagttcat cccataccgc accgaccgta gaacaggaga
    30721 attgcggata aagcggcagc accacaatat gatctacatg ctctgccagg agttcatcga
    30781 cggagctttc cagtgatggc gagccgtagc tcatgcccag cgccactggc gtatccggta
    30841 aacgttgtgc caacgcctgc tgttgctggc ggctgtaaac catcagcggc gagccgtctt
    30901 ccatccagac agaggcgtac agcttcgcca cacgcggcga gcgcagcggc aaaatcacgc
    30961 cgcgcagcaa cggccaccat aacaaccgtg aggtatcaac cacgcgtctg tcgcttaaaa
    31021 attgtttcag atagcgtttt accgcttcag gtgtgggggc atcgggcgta cccaggtttg
    31081 ccagcaggat accggtttta gtctgacgca ttaccgcctc ttatcgattc aacttgttga
    31141 taattgtagc ggaaaagtga ggaagaagaa ctaattgctg tgagaggtat tgcctgaggt
    31201 ggataagcag acctgcccga aagcagatct gctgcggctt gattagccga ggattttttc
    31261 cagatcagcg cgaacttcag caacctgctt ggtaccgtca actttcgcgt atttggtgtt
    31321 acccgcttcc gcttctttgg agtagtagcc gatcagcggt gcagtcatct gatggtattc
    31381 aaccagacgt ttacgcacgg tttcttcctg atcgtcttta cgggtagtca gttcttcacc
    31441 ggtaacgtcg tctttgcctt ctactttcgg cggattgaat ttaacgtgat aaacacgacc
    31501 agacggcgca tgaacgcggc ggcctacgat acgatcaaca atcagttcgt ccggtacgtc
    31561 gaattccaga acgtaatcaa cattgatgcc cgcttctttc atcgcgtctg cctgcggaat
    31621 ggtacgcggg aagccgtcca acaggaaacc attacggcag tcttcctgag caatgcgctc
    31681 tttaaccagc gcgatcacca gttcgtcggt gaccagtttg ccagcatcca taatgtcttt
    31741 tgcttgttta cccagctcgg agccagattt gaccgcagca cgcagcatat cgccagtgga
    31801 gatttgcgga ataccatatt tctccatgat gaactgagcc tgagtccctt tccccgcgcc
    31861 cggagcgcca agcagaatga tacgcattgc gaaaatcccc ttaaaatgtg tcgatttttt
    31921 tgaaaaagcg ataaacgata ccaccaacag gcgattgcct caagaaaggc acctgaggct
    31981 gaaacggtta atggcgagat aatttgcgga aagtacagat gatgaaaaga aaaatgccgg
    32041 atgacacaaa ggtcatccgg catgacatca ggaaaccagc agctgattca tacgacgaat
    32101 aaacaggttc ggatcttcca gcgtgccgcg ttctgccagc agcgcctgat ccagcagcag
    32161 ttctacccac tcgctgaact tggcttcatc ttcagtatct gccgcacgtt tcaccagtac
    32221 gtgatccggg ttcagttcga agatgtattt cacttctggc actttctggc ccgccgcagc
    32281 gaacagtttc gccatctgag tgctcatttc gtccgcgtcg gtcgaaacga tcgctggcgt
    32341 atcggtcaga cggtgagtca gacggacatc tttcacgcgc tcgccgagca gagctttcac
    32401 gcggtcgatg aacggagtca gtgctttctc cgcttctttc gcgctctcat caacttcgtc
    32461 agccagtttt tccagcgact cgtcaacttt agacaccgac tggaacggtt taccgtcgaa
    32521 ctcagtcaga tagttcatca tccactcatc gatgcggtcg gaaagcagca gaacttcgat
    32581 gcctttctta cgcagcagtt ccaggtgcgg gctgctcttc gctgccgcat agctgtctgc
    32641 agtgatgtag tagattttct cctgcccttc tttcatgcgg gaaacgtagt cttccagaga
    32701 cacggtctgc gcagaagaat cggtataagt agaagcaaaa cgcagcagtt tggcgatcgc
    32761 ttcctggtta gcgaaatctt ccgccggacc ttctttcagt accaggccaa actgttgcca
    32821 gaaggtctgg tatttttccg cgtcgtcttt cgccagtttt tccagcattt gcagcacacg
    32881 cttggtcagc gcattgcgca ggttacgcgt taccgtgctg tcctggagga tttcacggga
    32941 aacgttcagc ggcagatcgc tggagtcaat cagaccacgc acgaagcgca gatagttcgg
    33001 catgaactgt tcagcgtcgt ccatgataaa cacgcgttga acgtacagtt tcaggccgtg
    33061 tttatgatca cggttccaca tatcccacgg agcctgggac gggatgtaca gcaggctggt
    33121 gtactcctgc ttaccttcaa cgcggttgtg gctccaggtc agcggatcgt taaagtcgtg
    33181 ggcgatgtgt ttgtagaact ctttatactc ttcatcggtg atttccgact tgttacgagt
    33241 ccacagcgcc tgcgctttgt tgatttttgc ccaggagata acggtttcgc catctttctc
    33301 ttcgcgtttt tcgatctcta ccggcagcgc gatatggtcg gagtatttgc tgatgatgga
    33361 acgcacgcgc cagtcatcga ggaactcgtc ttcgccttca cgcagatgca gggtgatttc
    33421 agtaccacga tcttctttgg tgatatcggc aacggtatat tcaccttcgc cagccgattc
    33481 ccagaagacg ccgttttctg gtttttcgcc tgccgcgcga gtacgcacgg tcactttgtc
    33541 ggccacgata aacgcagagt agaaaccaac accaaactga ccgatcagct ggctgtcttt
    33601 cgcttggtca gaacccaggg attcgaggaa tgatttggta ccggatttag cgatagtccc
    33661 cagatggtca atcacttcgt cgcgggtcat ccccacgccg ttatcggaga tggtcagcgt
    33721 gcgcttgtct ttatcgaaag agacacgaac gcgcagttcg ccatcacctt cgtacaggtc
    33781 cgggttagat agcgcacgga aacgcagctt gtccgccgca tcggaggcgt tagagataag
    33841 ctcacgcagg aagatttctt tattggaata gagagaatgg atcatcaggt gcagaagctg
    33901 tttcacttct gactgaaaac cacgagtttc ttgtcctttc atgtaggtct acctcaataa
    33961 tgccatttta aggttaaaaa caggatgagg gagagatggg gacaagggag aataatttca
    34021 agcgagagca ggtgctcctg ctctcgtttg cttaaaaacg aatcttatga cgcccggcga
    34081 gggagtgtga cagcgtggtg ccatcgacca tttccagttc gccgccaacc ggtacgccat
    34141 gagcgattcg gctggcttcc acgtcatatt gcgcgcaaag ctcggcaatg tagttagcgg
    34201 tagcttcacc ttcaaccgtg ggattggtgg cgaggatcac ttcagtgatt ttttcctctg
    34261 ccagacgctg ttccagacga tcaagcccga tatcatccgg accgatgccg tccagcggtg
    34321 acagatgccc catcaacaca aaataacgac cagaaaactg ccccgtctgc tcaatggcgt
    34381 agatgtccgc cggactctcc accacacaga tttgaccgtt ttcctgacga cgcggattcg
    34441 aacagatgtt acagacttcc tgttcggtga aagtgcggca atcggcgcag tggccgattt
    34501 ccgacatcgc ccgggtgagc gcctgcgcca gacgcatccc gccgctacga tcgcgctgaa
    34561 gcagcgtgaa cgccatacgc tgcgccgact tcgggccaac gcccggcaga cagcgcagtg
    34621 cttccataag ctgtgttaac agcgggctgg tttgcatcag aacggcatct taaagccagg
    34681 cggcagctgc attccggagg atacagaggc cattttttct ttctgcgttt cttcaatacg
    34741 acgtgctgcg tcgttgaatg ctgcagccac caggtcttcc agcatctctt tgtcgtcttc
    34801 cagcaggctc gggtcgatct ctacgcgacg gcagttgtgt gcaccgttga tggtcacttt
    34861 taccagacct gcgccagatt cgccggtaac ttccagctgc gcgatctctt cctgcatttt
    34921 ctgcattttt tcttgcatct gctgggcttg cttcatcagg ttacccagac cgcctttacc
    34981 aaacataggt ttctctctca atcacgttaa ggatgacgaa cgtaagctgt gcttacgatc
    35041 aaatggggcg gatactttct tcatccagct ccgcatcgaa aaaccgacgc agggtctgaa
    35101 tattattatc cgcaataatg gactcgcgcg cctgcgcaag tttttcttcg tatatcgcct
    35161 gacgccactc cagcggcgta cgcaccgcgg gattatcatc ttcaacgata gtcagttcaa
    35221 ccgttgaacc ttttaacatg ctcaacgctt cagccagttt ttgctgtgca ccgcggttgt
    35281 tcaaatgccg ctgagaggag cgcaaatgca gacatactgc gttgtcgctc tcctctttcc
    35341 aggcatttaa cgccacctgt tcgaccagtt ttggtagcga aagttggctc acctgtgccg
    35401 cccacgggtc gcgctcaatg gcttctgccg ctagcttcac cgccagttcc ggcgtttttt
    35461 catgttccag cgcttttttc agcgccttcg gcgtggcgac cacttctttt tgctgcatca
    35521 ccggagtggt cgccttccag cgatacgctt cttttttggc tggcgctttt tccagcgccg
    35581 atggcaccgg acgcgcctga acgcgatcgg tgaccgaagc cagtctttcc agcgcagcgt
    35641 tattcaccgg ccgcgcgcgg gtagcggctg ccggttcact cttttttgct ttggttgctc
    35701 cctgcacgcg ctgcaactgc tggcgcgccg ccagcacctg gctggtggtt tccgggagcg
    35761 gtacagtcgg tgcctgctgc ggcgctgatt gcggcggcac cagggttggc gtcattactg
    35821 ccgttggcgc gacgggtgca aaggactggc gtggcacttc tggctcaggc agcggcatac
    35881 gcggatggaa cgccagcgcg cgcagcagcg tcatctcaac gcccatgcgg cggtccggcg
    35941 catacggtaa ttctttgcga ccaatcaaca gcgtctgata gtaaagctga atatccgtcg
    36001 gcggtatggt gcgcgccagt tcacgcatcc gcagctcgat ggcggccatg tcgttgccaa
    36061 gtgcagcagg cgaaagttgt accatcgcaa tacgatgcaa caagccgagc atttccacca
    36121 gcaacgcttc ccactcgata ccacgggcag cggcttcatt aatcagtgcc attacgcgct
    36181 cgccgttgcc ctcgaccatc gcttcaacca gcgacagcgc ctgatcgtcg tcaagcgtac
    36241 ccagcatcgc actgaccgcc tgggttgaaa cctggccgtc accgctggca atcgcctggt
    36301 cggtcagact taaggcatct cgcaggctgc cttcagcggc gcgcgccaac aattgcagcg
    36361 cccgcggctc gtgagcgata tgttcttcat tgaggatgtg ctcaagctga tggcgaattt
    36421 gctcgacatc cagcgccttg agatgaaatt gcagacagcg tgacaaaatc gtcaccggca
    36481 atttctgtgg atcggtcgtc gccagcagaa acttaacgtg ctccggcggc tcttcaaggg
    36541 tttttaacag tgcgttaaag ctgtggcgcg acagcatatg cacttcgtcg atcagataaa
    36601 ctttgaaacg accacgcgcc ggagcgtact ggacgttatc cagcaggtcg cgggtatctt
    36661 caactttggt gcgcgaggcg gcgtcgattt caatcagatc gacaaagcgc ccctgctcga
    36721 tttcacgaca gttatcgcac acgccgcacg gcgtcgcggt aatgccggtt tcgcagttta
    36781 gccccttcgc cagcagtcgg gcgatagagg tttttccgac gccacgggtg ccggaaaaaa
    36841 gataagcatg atgaatacgc cctaacgaca agccgttcgc cagtgcggtc agcacatgtt
    36901 cctggccgac gacgtcagca aaggtttgtg ggcgccattt tcgggctaaa acctgataac
    36961 tcattggcag gctctgaaac gctggaaggt ggattcacga aggggtaatg ctaacacagc
    37021 cccgtcagaa cggcgaggct gaatgcacat cttgtgctgt ccgtagcgtg ggcagcacaa
    37081 gactggcgat aattaatggc ccgggaacgg gacaaggctg tagctggtaa tgccctgttt
    37141 ttcgagacgc tgttcgccgc cgagatcaaa caggttgata atgaacgcag cgtcagccac
    37201 ttcaccaccc agacgacgga tcagtttaac ggtcgcttcg atagtgccgc cggttgccag
    37261 cagatcgtcc accaccagaa ctttgtcgcc cggtttgatg gcatcaacgt ggatttccag
    37321 ctgatcggtg ccgtattcca ggtcgtaatt ttcactgatg gtttcacgcg gcagtttgcc
    37381 cggtttacgg accggtacaa agccaacgcc cagacccaga gctaccggag cgccaaacaa
    37441 gaagccacgc gcttcggtgc cgacaacttt ggtaatgccc gcatttttgt aacgctcaac
    37501 cagcaagtcg atgctgagag cgtaagcttt cgggtcttcc agtaagctgg tgacatcgcg
    37561 gaaaagaatg ccgggttttg ggtagtcctg aatgcttttg atgctatttt tgagatactc
    37621 aagctgctgt gcagtcgcgg tcataagtgt atgcctgctt gttacggtgg tactcacggc
    37681 gcgttttaaa cgtatcaaaa gtaactgttg tttaaccttt gaccgcctgc gcctgtgctc
    37741 gaaaacgcca gaatgtactg gctgtacgca ataattgcaa ctgcgattgt tgtgcttcag
    37801 tgcttttctt gcttttcatc aatcaccgga attcgccaca tatagaaaag caaacaggcg
    37861 agaattacca gcaacatgat gcgcacccat ggcatctgga cgaaccacag agaaatggca
    37921 aacgtgagca aaataagcaa aatcgcccgc ggtttgacgc cgcgcggcat cgcatgatgt
    37981 ttctgccaga aacgtagata gctgccaaac catgagcggt acagcaacca ggcgtgaaag
    38041 cgcggggaag aacgggcaaa gcaccaggcc gccagcagga taaacggcgt cgtcggtaat
    38101 accggtaata ccacgcccag cgtacccagc actaccgcca gccagccaat gatgattaaa
    38161 atgattcgtt gcatattgag tgttgtcagc ttacatagtg gctactttag cataacaatt
    38221 atcattttca ttgaggtctt atcgtgaaaa ccgccctgct gctggaaaaa ctggaaggac
    38281 agctcgctac gctgcgtcag cgttgtgccc cggtgtcaca gttcgccacg ctaagtgctc
    38341 gtttcgacag gcatcttttt cagactcgtg cgacaacact acagtcttgt ctcgacgagg
    38401 cgggcgataa tctggctgcg cttcgtcatg cagttgagca gcaacagctg ccgcaagtgg
    38461 cctggctggc ggaacatctg gcggcacaac tggaagccat cgcgcgtgaa gcctccgcct
    38521 ggtcattgcg cgagtgggac agtgcgccac cgaaaatttc ccgctggcag cgtaaacgta
    38581 ttcagcatca ggattttgag cggcggctac gtgagatggt tgccgaacgc agagcccgcc
    38641 tggcgcgggt gaccgatctc gtggaacagc aaacgctgca tcgtgaagtg gaagcctatg
    38701 aagcgcgcct ggcacgctgc cgccatgcgc tggaaaaaat cgaaaacagg ctagcgcgct
    38761 taacccgcta gcaatggaaa gagtatgtca cttgaaaatg cccctgacga tgtcaaactg
    38821 gctgtcgatt tgattgtgct gctggaggaa aatcagatcc ctgccagtac cgtgctgcgc
    38881 gcgctggata ttgtaaagcg tgattatgaa aagaaattaa cgcacgatga tgaggcggaa
    38941 aagtgagaaa taaatggggc cgctaaatgt cccctaagat tgcgggcaaa cgcaaaactg
    39001 cctgatgcgc tacgcttatc aggcctacac cgctcctgca atatattgaa ttcttgcggt
    39061 ttcgtaggcc ggataaggcg gtcacgccgc atccggcatg aacaacgcgc actttgtcag
    39121 taatctcggg gcaactaaat gtcgccccct ttggttatta cccaaccgct ggtgtcggat
    39181 cgtcgccttt gtagtcgcgt tttacttccg tcacctcatc gcccttctcg ttgtgcagat
    39241 gcacttcaag ctggttaaag gcaatgttga tgtcgttttc acggcacagc tgatcgatag
    39301 tacggttcag ctcatcgaca gtacggctac ggtcacgcag ttcacgcaca tacagacgca
    39361 gctcatgatc caacgtgctg gcaccaaatg ccgtaaagaa gacttccggc attggttcgt
    39421 gcatcaccct tgggtgctca gtcgccgcct tcagtaacac tttacgcact ttttccagat
    39481 cggagccata ggccacgccg agacggatca ccagacgcgt agtagtgtca gtcaacgacc
    39541 agttgatcag acgctcggta acaaacgctt tgttcgggat gatcacttct ttgcgatcga
    39601 aatcggtaat cgttgtcgca cgaatacgga tcttacttac cgtccccgag aagctaccaa
    39661 tggttaccgt atcgccaata cgcaccggac gttcgaacag aatgatcaaa ccggagacga
    39721 agttaccgaa aatttcttgt aaaccaaaac caagacctac ggataatgct gcggccagcc
    39781 actggagttt atcccaagag acgcccagcg atccgaacac cgtcatcgca ccaacggcaa
    39841 taatgatgta gttaaggatg gtagtaatgg catacgacgc gccctggcgc atattcagtc
    39901 gcgagagcac cagcacttcc agtaaaccag gcaggttgcg aatcaacgcc caggccacca
    39961 ttgaggcgat aatcgcaaac aacagactgc ccatggtgac gtttttcacc accgcagcgc
    40021 cagcttcagt gccgttgtaa tgccagagcg tgatgctgtc gagatagctg aacacggtga
    40081 tcaaatcgga ccaaattgcc cagaacatga caccgaacag cgcaaacatc agcaacatgg
    40141 taatacgcag cgtctgctgg ttaacttgct ccagtgcaat ggtgggttct tccggcggtt
    40201 cggcaccttc tgcgccctct ttcaccagat tctgccgacg cgccagcgca cgacgccagg
    40261 cgatacgccg cgccgctacg cttaagccgc gcaataccgt ctggtagagc aagttccaga
    40321 tgatcaccag ataaacggtt tcaatccaac gcccggagag gcgcagcgtg gtgtagaaat
    40381 aacccgtggc agtcagcacc atcaatgcaa tcgggataat cgacagcacg gtaatggtga
    40441 ctagtcgcat ggtgtgcgac tctttatcac gccagctttc gcggcacatt ggccatacca
    40501 ggaaggcaat cagcagcagg ttgaagaaaa tcatcgcctg ccccagcaca tcatccatca
    40561 gatgcagcgg ggaaagttct gccaccacag accagaaatg gataggcagc aacgcgagac
    40621 tgatgcggac aatttgccga cgccagtggc tggtctgctg ctccggcatg ccgaagtgac
    40681 gcacggcaac gccatttttc tccagtacct tccagcacag gccaaacacc agccagaaaa
    40741 tcgccagttt tttactgaac gaccatagca gttcgctgat gttgagctgc atggtcaaca
    40801 gaatcaggcc aaccgcgaga ataatcaggc acaccggcag cgcgcggatc aggtcgataa
    40861 ggatcgcttt tggtgtgttg agctggctat cgttacgcag ggaacccaca gcggaagcca
    40921 gtttctgttg atacgctttc agccagccca gacgccagtg gatcagcccg gcaatcaaca
    40981 gcagcggcaa accggcgagg aaagcgataa aaacggcagg ccaggctttt tcccagttca
    41041 ccgtgatttt catcgactta aattcatctt tcaggctttg cgggaacgct ttgatccagt
    41101 cccagtccat tggacggtta ctgttcaccc aaaagatttg ctgagtcagg atggatttca
    41161 ggtttttcga tacgctcatt aactgctgct ggttgatttg caggttaatg gccatcatca
    41221 gctggttacc caactgtttg ttgagttgat ccagcaattc gcgacgcata tcaaccactt
    41281 gcaataacgc atcgtgaact tcgctgttga cttcgttggt gtgaccttct tccagtttgt
    41341 tgacgaacgc atcgctctgg aagagtgcat cacgctgctg gttaacttca aactgttcga
    41401 gacgcaaatc cgcgatgcgg ttggtcatgt tttccagttc atccgccgag ggcagcgttt
    41461 gttgttgctg gtaaaggata cgagacaaca gcaggctgcc cttcaggacg gcaatctgct
    41521 ctttaatatt gcgttccgat tgcagcgccc gctccagcca gtttttgact ttaatgtttt
    41581 gctgcatcaa ctgattaccg ttttcagtcg cggtaatcag acgctgactt aactgctggt
    41641 taatttccag ttcctgtttc accagcggat tagcctgaat acgcgcggct tcatccgggg
    41701 agacggcttc ctgcgccgtt ttttcagtta aagtcaggcg cttgctgttt accgcttctt
    41761 gcaacagctg caactggtgc tccagacggg cgctgttcgc cgtcacgtaa tcacgttgct
    41821 tttgcaaggt atcctgcaag acggtgttcc cttccaggct tttacgctgc tggtcaatct
    41881 ccgcattcag caacgcctgc tgagcctgca ttaacacttt ctggctggga cgtaaggcag
    41941 tctcgccgac atcagtccca tccagacgac tgcgaatttg ttgcagctgc tgcgaagcgt
    42001 tatacatcgc attttgcacg cgttcgggct gcgtctgtaa cgaaaccagc tggctgttat
    42061 aagacgccag atcgttttgt gcgttttgca aatcgtccag cgcctgggca acgcgagttt
    42121 ccagctggcg caacgaaagc gtactcagaa ttttgcgcgt ttcttcgtcg ttatcgacat
    42181 cgctaagtgc tgttaacgcc gcggtcgcct ggcgcatttt ttccggcgct tcagcgactt
    42241 tttgccgtag ctgaactgtc tcttctttta tgcgatcgat tttatcgagg gtggctaatg
    42301 tatctgtcag atcctgctgc accagtttgt cctgagcaga aagatctttt tgtttattta
    42361 gtgagtcaag ttgcgcctgc aggtccgctt ttgtcggcag atcaccattc gatgacgccc
    42421 gcgcaaacgc cgtgttctgg cataacaaga caaaaacaaa agcaataaat gctgaaaaaa
    42481 caaaatgccg tgatcgtttg taatactgga acatagtcat gatgaatgaa ggtttctgaa
    42541 cctgaagaac gacctgaaaa agtcaaaccg caagaatatc acgacgcagt gaaccagaat
    42601 agcaacgacg aaaatgtcca ggaaaaatcc tggagtcaga ttcagggtta ttcgttagtg
    42661 gcaggattac gaagcgtggg gcacaggaga tacatctcca gtaagatggc gacgtaatcg
    42721 cgggcttctt ttttaagatc aaaagattgc ggggcaaaga gccagttttc catcaggccg
    42781 gaaatatagc cgcgcataat aattgctgcc cgacgcgtca ttaaatccgc aggcaacatt
    42841 ttcgcttcaa tacaatgttt taacgtttgt tctatacggt cataactttc cagacagaga
    42901 ttacgttgtg cctgttgcac aacagccatt tctccgacaa attcgcattt gtggaatata
    42961 atctccatca ataatcgacg tcgttcttct gtcaccgtgg attcaagaac atgaattaat
    43021 atctctctta atactgagag tggatcgcca gggaattttg cctgatactc aagctctagt
    43081 tcaccaatat tggattctga cagttcccag atctcactga acaaatccga cttgtcttta
    43141 aaatgccagt agattgcacc gcgcgtaacg ccagctgctt ttgcaatctc gcccagcgag
    43201 gtggatgata ccccctgctg tgagaaaaga cgtagagcca catcgaggat gtgttggcgc
    43261 gtttcttgcg cttcttgttt ggtttttcgt gccatatgtt cgtgaattta caggcgttag
    43321 atttacatac atttgtgaat gtatgtacca tagcacgacg ataatataaa cgcagcaatg
    43381 ggtttattaa cttttgacca ttgaccaatt tgaaatcgga cactcgaggt ttacatatga
    43441 acaaaaacag agggtttacg cctctggcgg tcgttctgat gctctcaggc agcttagccc
    43501 taacaggatg tgacgacaaa caggcccaac aaggtggcca gcagatgccc gccgttggcg
    43561 tagtaacagt caaaactgaa cctctgcaga tcacaaccga gcttccaggt cgcaccagtg
    43621 cctaccggat cgcagaagtt cgtcctcaag ttagcgggat tatcctgaag cgtaatttca
    43681 aagaaggtag cgacatcgaa gctggtgtct ctctctatca gattgatcct gcgacctatc
    43741 aggcgacata cgacagtgcg aaaggtgatc tggcgaaagc ccaggctgca gccaatatcg
    43801 cgcaattgac ggtgaatcgt tatcagaaac tgctcggtac tcagtacatc agtaagcaag
    43861 ggtacgatca ggctctggct gatgcgcaac aggcgaatgc tgcggtaact gcggcgaaag
    43921 ctgccgttga aactgcgcgg atcaatctgg cttacaccaa agtcacctct ccgattagcg
    43981 gtcgcattgg taagtcgaac gtgacggaag gcgcattggt acagaacggt caggcgactg
    44041 tgctggcaac cgtgcagcaa cttgatccga tctacgttga tgtgacccag tccagcaacg
    44101 acttccttcg cctgaaacag gaactggcga atggcacgct gaaacaagag aacggcaaag
    44161 ccaaagtgtc gctgatcacc agtgacggca ttaagttccc gcaggacggt acgctggaat
    44221 tctctgacgt taccgttgat cagaccactg ggtctatcac cctacgcgct atcttcccga
    44281 acccggatca cactctgctg ccgggtatgt tcgtgcgcgc acgtctggaa gaagggctta
    44341 atccaaacgc tattttagtc ccgcaacagg gcgtaacccg tacgccgcgt ggtgatgcca
    44401 ccgtactggt agttggcgcg gatgacaaag tggaaacccg tccgatcgtt gcaagccagg
    44461 ctattggcga taagtggctg gtgacagaag gtctgaaagc aggcgatcgc gtagtaataa
    44521 gtgggctgca gaaagtgcgt cctggtgtcc aggtaaaagc acaagaagtt accgctgata
    44581 ataaccagca agccgcaagc ggtgctcagc ctgaacagtc caagtcttaa cttaaacagg
    44641 agccgttaag acatgcctaa tttctttatc gatcgcccga tttttgcgtg ggtgatcgcc
    44701 attatcatca tgttggcagg ggggctggcg atcctcaaac tgccggtggc gcaatatcct
    44761 acgattgcac cgccggcagt aacgatctcc gcctcctacc ccggcgctga tgcgaaaaca
    44821 gtgcaggaca cggtgacaca ggttatcgaa cagaatatga acggtatcga taacctgatg
    44881 tacatgtcct ctaacagtga ctccacgggt accgtgcaga tcaccctgac ctttgagtct
    44941 ggtactgatg cggatatcgc gcaggttcag gtgcagaaca aactgcagct ggcgatgccg
    45001 ttgctgccgc aagaagttca gcagcaaggg gtgagcgttg agaaatcatc cagcagcttc
    45061 ctgatggttg tcggcgttat caacaccgat ggcaccatga cgcaggagga tatctctgac
    45121 tacgtagcgg cgaatatgaa agatgccatc agccgtacgt cgggcgtggg tgatgttcag
    45181 ttgttcggtt cacagtacgc gatgcgtatc tggatgaacc cgaatgagct gaacaaattc
    45241 cagctaacgc cggttgatgt cattaccgcc atcaaagcgc agaacgccca ggttgcggcg
    45301 ggtcagctcg gtggtacgcc gccggtgaaa ggccaacagc ttaacgcctc tattattgct
    45361 cagacgcgtc tgacctctac tgaagagttc ggcaaaatcc tgctgaaagt gaatcaggat
    45421 ggttcccgcg tactgctgcg tgatgtggcg aagattgagc tgggtggtga gaactacgac
    45481 atcatcgcag agtttaacgg ccaacctgct tccggtctgg ggatcaagct ggcgaccggt
    45541 gcaaacgcgc tggataccgc tgcggcaatc cgtgctgaac tggcgaagat ggaaccgttc
    45601 ttcccgtcgg gtctgaaaat tgtttacccg tacgacacca cgccgttcgt gaaaatctct
    45661 attcacgaag tggttaaaac gctggtcgaa gcgatcatcc tcgtgttcct ggttatgtat
    45721 ctgttcctgc agaacttccg cgcgacgttg attccgacca ttgccgtacc ggtggtattg
    45781 ctcgggacct ttgccgtcct tgccgccttt ggcttctcga taaacacgct aacaatgttc
    45841 gggatggtgc tcgccatcgg cttgttggtg gatgacgcta tcgttgtggt agaaaacgtg
    45901 gagcgtgtta tggcggaaga aggtttgccg ccaaaagaag ctacccgtaa gtcgatgggg
    45961 cagattcagg gcgctctggt cggtatcgcg atggtactgt cggcggtatt cgtaccgatg
    46021 gccttctttg gcggttctac tggtgctatc tatcgtcagt tctctattac cattgtttca
    46081 gcaatggcgc tgtcggtact ggtagcgttg atcctgactc cggctctttg tgccaccatg
    46141 ctgaaaccga ttgccaaagg cgatcacggg gaaggtaaaa aaggcttctt cggctggttg
    46201 aaccgcatgt tcgagaagag cacgcaccac tacaccgaca gcgtaggcgg tattctgcgc
    46261 agtacggggc gttacctagt gctgtatctg atcatcgtgg tcggcatggc ctatctgttc
    46321 gtgcgtctgc caagctcctt cttgccagat gaggaccagg gcgtatttat gaccatggtt
    46381 cagctgccag caggtgcaac gcaggaacgt acgcagaaag tgctcaatga ggtaacgcat
    46441 tactatctga ccaaagaaaa gaacaacgtt gagtcggtgt tcgccgttaa cggcttcggc
    46501 tttgcgggac gtggtcagaa taccggtatt gcgttcgttt ccttgaagga ctgggccgat
    46561 cgtccgggcg aagaaaacaa agttgaagcg attaccatgc gtgcaacacg cgctttctcg
    46621 caaatcaaag atgcgatggt tttcgccttt aacctgcccg caatcgtgga actgggtact
    46681 gcaaccggct ttgactttga gctgattgac caggctggcc ttggtcacga aaaactgact
    46741 caggcgcgta accagctgct tgcagaagca gcgaagcacc ctgatatgct gaccagcgta
    46801 cgtccaaacg gtctggaaga taccccgcag tttaagattg atatcgacca ggaaaaagcg
    46861 caggcgctgg gtgtttctat caacgacatt aacaccactc tgggcgctgc atggggcggt
    46921 agctatgtga acgactttat cgaccgcggt cgtgtgaaga aagtttacgt catgtcagaa
    46981 gcgaaatacc gtatgctgcc ggatgatatc ggcgactggt atgttcgtgc tgctgatggt
    47041 cagatggtgc cattctcggc gttctcctct tctcgttggg agtacggttc gccgcgtctg
    47101 gaacgttaca acggcctgcc atccatggaa atcttaggcc aggcggcacc gggtaaaagt
    47161 accggtgaag caatggagct gatggaacaa ctggcgagca aactgcctac cggtgttggc
    47221 tatgactgga cggggatgtc ctatcaggaa cgtctctccg gcaaccaggc accttcactg
    47281 tacgcgattt cgttgattgt cgtattcctg tgtctggcgg cgctgtacga gagctggtcg
    47341 actccgttct ccgttatgct ggtcgttccg ctgggggtta tcggtgcgtt gctggctgcc
    47401 accttccgtg gcctgaccaa tgacgtttac ttccaagtag gactgctcac aaccattggg
    47461 ttgtcggcga agaacgcgat acttatcgtc gaattcgcca aagacttgat ggataaagaa
    47521 ggtaaaggtc tgattgaagc gacgcttgat gcggtgcgga tgcgtttacg tccgatcctg
    47581 atgacctcgc tggcgtttat cctcggcgtt atgccgctgg ttatcagtac tggtgctggt
    47641 tccggcgcgc agaacgcagt aggtaccggt gtaatgggcg ggatggtgac cgcaacggta
    47701 ctggcaatct tcttcgttcc ggtattcttt gtggtggttc gccgccgctt tagccgcaag
    47761 aatgaagata tcgagcacag ccatactgtc gatcatcatt gatacaacat gtaatcacta
    47821 aggccgcgta agcggccttt tttatgcata acctacgaac attaaggagt aattgaacca
    47881 ccaactcagg atctcatacg aaaaccagta ttaaccacgt ataaaattca taaaaaatac
    47941 tgattgttag ttaatttata ttaagtagcg ctaatagatt taataatcca taatcattta
    48001 gaggctattc ttaattattt gcggtaattc tttattcatt cctcggttat tacgtcatat
    48061 tcagagcaat cctggtatta gtgtcaccaa tttcatctgg cgataatcct gaaatgttat
    48121 gaacagttcg agcaaactgc ttttacctgc tgcgggttag tgctagtatg aaaaagtgag
    48181 tcctgtcccg attccttcct aattgtaatg tttcgtaata atgcgatgaa aacctgcaaa
    48241 gagtggctta tagttaagct aacaaacgag agggcaagtc taggtcagta agttttttcc
    48301 atcccgaaag gtgtccgtta gttcaaccac taagaagggg acgcgttatg gatgaatact
    48361 cacccaaaag acatgatatc gcacagctta agtttctctg tgaaaccctg tatcatgact
    48421 gtcttgcaaa ccttgaagaa agcaatcatg gttgggttaa cgacccaacc tcggcgatca
    48481 acctccagtt gaatgaactg attgagcata ttgcgacctt cgcacttaat tacaaaatta
    48541 agtataatga agacaataag ctcattgagc agatcgacga atatctggat gacaccttta
    48601 tgttgttcag cagttatggt attaatatgc aggctcttca gaaatggcgg aagtcaggta
    48661 atcgactatt ccgttgtttt gtcaacgcga cgaaagagaa tcctgcgagt ttatcttgtt
    48721 agaattatta caaccatggg tagaagtatg tccgaaaaac ctttaacaaa aaccgattat
    48781 ttaatgcgtt tacgtcgttg ccagacaatt gacacgctgg agcgtgttat cgagaaaaat
    48841 aaatacgaat tatcagataa tgaactggcg gtattttact cagccgcaga tcaccgcctc
    48901 gccgaattga ccatgaataa actgtacgac aagatccctt cctcagtatg gaaatttatt
    48961 cgctaataaa taattcgctt tcggtgctat aaccggctgt ttattaagaa ttttatactt
    49021 tttcgccatg aagacatacc ctatgtgatc tttatcacac agatgtaatg agaacgttct
    49081 cttcactgac ttttcgtctt actgtgttgc cgcattttca gcaaccggag gtcagtaatg
    49141 agcacagaaa aagaaaagat gattgctggt gagttgtatc gctcggcaga tgagacgtta
    49201 tctcgcgatc gcctgcgcgc tcgtcagctt attcaccgat acaatcattc cctggcggaa
    49261 gagcacacat tacgccagta aattctcgct aatttattcg gtcaggtgac agaggcttat
    49321 attgagccaa cgtttcgctg tgattatggc tataacattt ttctcggcaa taattttttc
    49381 gccaacttcg attgcgtgat gcttgatgtc tgccctattc gcatcggtga taactgtatg
    49441 ttggcaccag gcgttcatat ctacacggca acacatccca tcgaccctgt agcacgtaat
    49501 agcggtgctg aactggggaa acccgtcacc atcggtaata acgtctggat tggcggacgc
    49561 gcggtcatta acccttgtgt gaccattggt gataacgtcg tggtagcttc aggtgcagtt
    49621 gtcacaaaag gtgtcccgga caacgttgtc gtgggcggta atccagccag aataattaaa
    49681 aaattgtaat cggtttttcg caactgttat acaaaattgt ggtagatctg ttacttcccc
    49741 tctactattc ccacgttaaa atagggtgtt ccctggaaag ttgcagatac cacgaaggca
    49801 aacgatgacc gaaatacaac gcctgctgac cgaaacgatt gagtctctga atacccgcga
    49861 aaaacgcgac aacaaacccc gctttagtat cagttttatc cgtaaacatc cggggctgtt
    49921 tatcggtatg tacgttgctt tttttgccac cctggcggtg atgttgcagt ccgaaacgct
    49981 gtcaggctct gtctggctac tggttgtatt atttatcctg cttaatggtt tcttcttttt
    50041 cgatgtctac ccacgctacc gctatgaaga tatcgacgtg ctggatttcc gcgtttgcta
    50101 taacggcgaa tggtacaaca cgcgctttgt acctgccgcg ctggttgaag ccatcttgaa
    50161 ctctccgcgt gtcgcggatg ttcataagga acaactgcaa aaaatgatcg tccgtaaagg
    50221 tgaactgtct ttttacgata tttttaccct cgctcgcgcc gaatcaacat cttaagttag
    50281 ggttacatac caggcgtaaa gctctgcgcc tggtcaaatg acaatgatcg tttccaccca
    50341 tcacttcatg aaataccagc tctacctcct tatctcctgc cagccttttt ccacaatcag
    50401 atatactttc cctacactgt gttaataagg atatgctggt gagaacacga catctggtcg
    50461 gccttatttc gggagtactg attctttcag tattgctgcc tgtcggctta agcatctggc
    50521 tggcccatca gcaggtagaa acatcgttta ttgaagagct ggatacctat tcctcccgcg
    50581 tcgctattcg agccaataag gtggcgacac aagggaaaga tgcgctgcag gagctggaaa
    50641 gatggcaagg cgctgcctgt agcgaagccc atctcatgga aatgcgtcgg gtatcttaca
    50701 gttatcgcta tattcaggaa gtggtttata tcgataacaa cgttccccag tgttcgtctc
    50761 tggagcatga aagtccgccc gatacctttc ccgagctagg taaaatttcg aaagatggtt
    50821 atcgtgtctg gttaacatcg cataacgatt taggcattat ccgttacatg gtcgccatgg
    50881 gaacggcaca ttatgtcgtc atgatcgacc ccgcttcctt tattgatgtc attccctata
    50941 gttcatggca aattgatgcc gccattattg gcaatgccca taacgttgtc ataaccagca
    51001 gcgatgaaat tgctcaggga attattacca ggctacaaaa aacacccggt gagcatatcg
    51061 aaaataatgg gatcatttac gatatactgc ccttcctgga gatgaatatt tcgatcatca
    51121 cctgggcttc aacgaaaatg ttgcagaaag gctggcatcg gcaagtcttt atttggttac
    51181 cgctcgggtt ggtgattggc ctgctggcag cgatgtttgt gctgcgtatt ttgcgccgta
    51241 tccaatcacc gcatcatcgg ctgcaggatg ctatcgaaaa tcgtgatatt tgcgtgcact
    51301 atcagccgat tgtctcctta gccaatggca aaattgtcgg tgctgaggca ctggcgcgct
    51361 ggccgcagac agacggtagt tggttgtcac cagatagttt tattccgctg gcacagcaaa
    51421 cgggcctttc tgagccattg acgctactga ttataagaag cgtctttgaa gatatgggcg
    51481 actgcttgcg tcagcatcca cagcagcata tttcgatcaa tcttgaatcc accgtgctca
    51541 cctcggaaaa aatcccgcaa ttgctgcgtg aaatgatcaa tcactatcag gttaatccca
    51601 gacagatcgc gcttgaactc actgaacgcg agtttgccga tccgaaaacc agcgccccga
    51661 taatttctcg ctaccgggag gcgggccatg aaatttatct tgatgatttt ggtacggggt
    51721 attcaagttt aagttattta caggatctgg atgtcgacat tctgaagatc gataaatctt
    51781 tcgttgatgc gctggaatat aaaaatgtca cgccgcatat catcgaaatg gcaaaaacac
    51841 tgaaactgaa aatggtagcg gagggaatcg aaaccagtaa acaagaagag tggctacgcc
    51901 agcatggcgt gcactacggt cagggctggc tctacagcaa ggcattaccg aaagaagatt
    51961 tcttacgctg ggccgagcaa catttgtgaa tttttgggct gacggcacgc cgcacagccc
    52021 gtcacgctac ctattcgtcg atgattgact cgaatccgcc atagatcatt cgcttgccgt
    52081 caaacggcat ggactcgccg aactctttca tccgtgggtc cgacatcatc ttttgattag
    52141 ctgcgtcgcg gacctctttt gaagggtatt caatccagct aaagacaacc tcttcattct
    52201 cttccgcttt caccgccata cgaaaatcgg tcactttgcc atccggcaca tcgctggccc
    52261 agcattcgac aatacgaagc gcgccaaact ctttaaacaa tggcgcggcc ttagcggcca
    52321 tttcccgata ggcatccttt ttatcggcag gaacggcaac cacaaaacca tcaacatact
    52381 tcatcagaca tacctccgaa gcgtactgcg cagccagaca attcttgcga cgtcagttgt
    52441 ggttaaagtt tagccatgct gggatttttt gacgaaacgg tgggaggcag aaatgggtgg
    52501 gggccctgcc agctacatcc c
//
LOCUS       DAJVPT010000013        52321 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVPT010000013 DAJVPT010000000
VERSION     DAJVPT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..540)
                     /locus_tag="OQM47_000773"
     CDS             complement(<1..540)
                     /locus_tag="OQM47_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1479041.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVI"
     gene            694..1845
                     /gene="argE"
                     /locus_tag="OQM47_000774"
     CDS             694..1845
                     /gene="argE"
                     /locus_tag="OQM47_000774"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1479042.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            2197..4848
                     /gene="ppc"
                     /locus_tag="OQM47_000775"
     CDS             2197..4848
                     /gene="ppc"
                     /locus_tag="OQM47_000775"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1479043.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            5031..6764
                     /locus_tag="OQM47_000776"
     CDS             5031..6764
                     /locus_tag="OQM47_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1479044.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            6913..7764
                     /locus_tag="OQM47_000777"
     CDS             6913..7764
                     /locus_tag="OQM47_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1479045.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(7751..8092)
                     /locus_tag="OQM47_000778"
     CDS             complement(7751..8092)
                     /locus_tag="OQM47_000778"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1479046.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(8094..8972)
                     /locus_tag="OQM47_000779"
     CDS             complement(8094..8972)
                     /locus_tag="OQM47_000779"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1479047.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(8938..11235)
                     /locus_tag="OQM47_000780"
     CDS             complement(8938..11235)
                     /locus_tag="OQM47_000780"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1479048.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(11286..11606)
                     /locus_tag="OQM47_000781"
     CDS             complement(11286..11606)
                     /locus_tag="OQM47_000781"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1479049.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(11621..12700)
                     /locus_tag="OQM47_000782"
     CDS             complement(11621..12700)
                     /locus_tag="OQM47_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1479050.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            13009..14112
                     /locus_tag="OQM47_000783"
     CDS             13009..14112
                     /locus_tag="OQM47_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1479051.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            14113..15510
                     /gene="ptsP"
                     /locus_tag="OQM47_000784"
     CDS             14113..15510
                     /gene="ptsP"
                     /locus_tag="OQM47_000784"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1479052.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            15522..16184
                     /gene="fsa"
                     /locus_tag="OQM47_000785"
                     /pseudo
     CDS             15522..16184
                     /gene="fsa"
                     /locus_tag="OQM47_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            16195..17296
                     /gene="gldA"
                     /locus_tag="OQM47_000786"
                     /pseudo
     CDS             16195..17296
                     /gene="gldA"
                     /locus_tag="OQM47_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            17571..18178
                     /locus_tag="OQM47_000787"
                     /pseudo
     CDS             17571..18178
                     /locus_tag="OQM47_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(18205..19110)
                     /gene="yijE"
                     /locus_tag="OQM47_000788"
     CDS             complement(18205..19110)
                     /gene="yijE"
                     /locus_tag="OQM47_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1479053.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(19204..21384)
                     /gene="katG"
                     /locus_tag="OQM47_000789"
     CDS             complement(19204..21384)
                     /gene="katG"
                     /locus_tag="OQM47_000789"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1479054.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(21713..22603)
                     /gene="metF"
                     /locus_tag="OQM47_000790"
     CDS             complement(21713..22603)
                     /gene="metF"
                     /locus_tag="OQM47_000790"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1479055.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(22952..25384)
                     /gene="metL"
                     /locus_tag="OQM47_000791"
     CDS             complement(22952..25384)
                     /gene="metL"
                     /locus_tag="OQM47_000791"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1479056.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(25387..26547)
                     /gene="metB"
                     /locus_tag="OQM47_000792"
     CDS             complement(25387..26547)
                     /gene="metB"
                     /locus_tag="OQM47_000792"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1479057.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            26824..27141
                     /gene="metJ"
                     /locus_tag="OQM47_000793"
     CDS             26824..27141
                     /gene="metJ"
                     /locus_tag="OQM47_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1479058.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            27325..27933
                     /locus_tag="OQM47_000794"
     CDS             27325..27933
                     /locus_tag="OQM47_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1479059.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(27994..28206)
                     /gene="rpmE"
                     /locus_tag="OQM47_000795"
     CDS             complement(27994..28206)
                     /gene="rpmE"
                     /locus_tag="OQM47_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1479060.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            28409..30607
                     /gene="priA"
                     /locus_tag="OQM47_000796"
     CDS             28409..30607
                     /gene="priA"
                     /locus_tag="OQM47_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1479061.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            30763..31788
                     /gene="cytR"
                     /locus_tag="OQM47_000797"
     CDS             30763..31788
                     /gene="cytR"
                     /locus_tag="OQM47_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1479062.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            31880..32839
                     /gene="ftsN"
                     /locus_tag="OQM47_000798"
     CDS             31880..32839
                     /gene="ftsN"
                     /locus_tag="OQM47_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1479063.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            32932..33462
                     /gene="hslV"
                     /locus_tag="OQM47_000799"
     CDS             32932..33462
                     /gene="hslV"
                     /locus_tag="OQM47_000799"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1479064.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            33472..34803
                     /gene="hslU"
                     /locus_tag="OQM47_000800"
     CDS             33472..34803
                     /gene="hslU"
                     /locus_tag="OQM47_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1479065.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            34870..35796
                     /gene="menA"
                     /locus_tag="OQM47_000801"
     CDS             34870..35796
                     /gene="menA"
                     /locus_tag="OQM47_000801"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1479066.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            35889..36374
                     /gene="rraA"
                     /locus_tag="OQM47_000802"
     CDS             35889..36374
                     /gene="rraA"
                     /locus_tag="OQM47_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1479067.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(36459..36704)
                     /gene="zapB"
                     /locus_tag="OQM47_000803"
     CDS             complement(36459..36704)
                     /gene="zapB"
                     /locus_tag="OQM47_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1479068.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            37130..37975
                     /gene="glpF"
                     /locus_tag="OQM47_000804"
                     /pseudo
     CDS             37130..37975
                     /gene="glpF"
                     /locus_tag="OQM47_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            38015..39505
                     /gene="glpK"
                     /locus_tag="OQM47_000805"
     CDS             38015..39505
                     /gene="glpK"
                     /locus_tag="OQM47_000805"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1479069.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            39735..40745
                     /gene="glpX"
                     /locus_tag="OQM47_000806"
     CDS             39735..40745
                     /gene="glpX"
                     /locus_tag="OQM47_000806"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1479070.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            40842..41588
                     /gene="fpr"
                     /locus_tag="OQM47_000807"
     CDS             40842..41588
                     /gene="fpr"
                     /locus_tag="OQM47_000807"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1479071.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(41593..42021)
                     /gene="uspD"
                     /locus_tag="OQM47_000808"
     CDS             complement(41593..42021)
                     /gene="uspD"
                     /locus_tag="OQM47_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1479072.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(42048..42347)
                     /locus_tag="OQM47_000809"
     CDS             complement(42048..42347)
                     /locus_tag="OQM47_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1479073.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(42559..42999)
                     /locus_tag="OQM47_000810"
                     /pseudo
     CDS             complement(42559..42999)
                     /locus_tag="OQM47_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            43100..43699
                     /locus_tag="OQM47_000811"
     CDS             43100..43699
                     /locus_tag="OQM47_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1479074.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            43807..44574
                     /gene="tpiA"
                     /locus_tag="OQM47_000812"
     CDS             43807..44574
                     /gene="tpiA"
                     /locus_tag="OQM47_000812"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1479075.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(44629..45384)
                     /gene="cdh"
                     /locus_tag="OQM47_000813"
     CDS             complement(44629..45384)
                     /gene="cdh"
                     /locus_tag="OQM47_000813"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1479076.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(45491..46480)
                     /gene="sbp"
                     /locus_tag="OQM47_000814"
     CDS             complement(45491..46480)
                     /gene="sbp"
                     /locus_tag="OQM47_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1479077.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(46800..47762)
                     /gene="pfkA"
                     /locus_tag="OQM47_000815"
     CDS             complement(46800..47762)
                     /gene="pfkA"
                     /locus_tag="OQM47_000815"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1479078.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(47943..48845)
                     /gene="fieF"
                     /locus_tag="OQM47_000816"
     CDS             complement(47943..48845)
                     /gene="fieF"
                     /locus_tag="OQM47_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1479079.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(48993..49493)
                     /gene="cpxP"
                     /locus_tag="OQM47_000817"
     CDS             complement(48993..49493)
                     /gene="cpxP"
                     /locus_tag="OQM47_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1479080.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            49643..50341
                     /gene="cpxR"
                     /locus_tag="OQM47_000818"
     CDS             49643..50341
                     /gene="cpxR"
                     /locus_tag="OQM47_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1479081.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            50338..51711
                     /gene="cpxA"
                     /locus_tag="OQM47_000819"
     CDS             50338..51711
                     /gene="cpxA"
                     /locus_tag="OQM47_000819"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1479082.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(51817..>52321)
                     /gene="yiiM"
                     /locus_tag="OQM47_000820"
     CDS             complement(51817..>52321)
                     /gene="yiiM"
                     /locus_tag="OQM47_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1479083.1"
                     /translation="CHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMG
                     DIFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAP
                     LELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDF
                     SRRLWGK"
BASE COUNT        12813 a        14067 c        13426 g        12015 t
ORIGIN      
        1 gatcaccggc cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag
       61 ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt
      121 accgcaccac tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt
      181 aaagccgtaa tatttttcat agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc
      241 gaacaccacg cagcccgctt caagaaactg cggcgctaaa tcgtggctaa cttcatgggc
      301 ggtggcgaga aacactacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg
      361 caacggcagc tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc
      421 atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac
      481 ataggtcact agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat
      541 cggggctatt caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg
      601 gctggtgggt tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat
      661 attgatacta tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc
      721 gagatttacc gcgctctgat tgccacacct tcaataagcg ctacggaaga ggcactcgat
      781 caaagcaatg cagatttaat cactctgctg gcggactggt ttaaagattt gggcttcaat
      841 gtggaagtgc agcctgttcc aggaactcgc aacaaattta atatgctggc cagttacgga
      901 cagggggctg gcggcttgtt gctggcgggg cataccgata cggtgccatt tgatgacggt
      961 cgctggacgc gcgatccgtt tacactgacg gagcatgacg gcaagcttta cggcttaggc
     1021 accgccgaca tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tgtcgacgtc
     1081 acgaaactga aaaaaccgct ctacattctg gcgactgctg atgaagaaac cagtatggcc
     1141 ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgaa
     1201 ccgacgtcac tacaaccggt acgcgcacat aaaggtcata tctctaacgc catccgtatt
     1261 cagggccagt cggggcactc cagcgatcca gcacgcggaa ttaacgctat cgaactaatg
     1321 cacgacgcca tcgggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac
     1381 gaagcattta ccgtgccata ccctacgctc aacctcgggc atattcacgg tggcgacgct
     1441 tctaaccgta tttgcgcttg ctgtgagttg catatggata ttcgtccgca gcctggcatg
     1501 acactcaatg aacttaatgg tttgctcaac gatgcattgg ctccggtgag cgaacgctgg
     1561 ccgggtcgtc tgacggtcga cgagctgcat ccgccgatcc ctggctatga atgcccaccg
     1621 aatcatcaac tggttgaagt ggttgagaaa ttgctcggag caaaaaccga agtggtgaat
     1681 tactgtaccg aagcgccgtt tattcaaacg ttatgcccga cgctggtgct cggtccgggt
     1741 tccatcaatc aagctcatca gcctgatgaa tatctggaga cgcggtttat taaacccacc
     1801 cgtgagttga ttatccaggt aattcaccat ttttgctggc attaaaacgt aggccggata
     1861 aggcgctcgc gccgcatccg gcaccgttgc caaactccgg tgccgcaata atgtcggatg
     1921 cgatgcttgc gcatcttatc cgacctacac ctttggtgtt atgtatggcg attttttaac
     1981 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
     2041 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
     2101 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
     2161 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
     2221 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
     2281 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt cgaaatcttc acgcgctggc
     2341 aatgatgcta accgccagga gttgctcacc accttacaaa atttgtcgaa cgacgagctg
     2401 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
     2461 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
     2521 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
     2581 ctgtcgctgg aactggtcct cacggctcac ccaaccgaaa ttacccgtcg tacactgatc
     2641 cacaaaatgg tggaagtgaa cgcctgttta aaacagctcg ataacaaaga tatcgctgac
     2701 tacgagcgca accagctgat gcgtcgcctg cgccagttga tcgcccagtc atggcatacc
     2761 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
     2821 gtagaaaaca gcctgtggca aggcgtaccg aattacctgc gcgaactgaa cgaacaactg
     2881 gaagagaatc tcggctacaa actgcccgtc gaatttgttc cggtacgttt tacctcgtgg
     2941 atgggcggtg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
     3001 ctgctcagcc gctggaaagc caccgatctg ttcctgaaag atattcaggt tctggtttct
     3061 gaactgtcga tggttgaagc gacccctgaa ctgctggcgc tggttggcga agaaggtgcc
     3121 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
     3181 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaagggct gctgacacaa
     3241 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
     3301 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
     3361 ccgctggtcc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
     3421 ctgacccgct acctcggtat cggcgactac gaaagctggt cagaggccga caaacaggcg
     3481 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
     3541 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
     3601 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
     3661 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
     3721 gatgacctga acaacgccaa cgatgtcatg acccagctgc tcaatatcga ctggtatcgc
     3781 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
     3841 ggcgtgatgg cagcttcctg ggcgcaatat caggcacagg atgcattaat caaaacctgc
     3901 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
     3961 ggcgcacctg cccatgcggc gctgctgtca caaccgccag gaagcctgaa aggtggcctg
     4021 cgcgtgactg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
     4081 agcagcctgt cgctttacac cggggcgatt ctggaagcca acctgctgcc accacctgag
     4141 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatctctac
     4201 cgtggctacg tacgtgaaaa caaagatttt gtgccttact tccgctccgc tacgccggaa
     4261 caagaattgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
     4321 gtcgagtcgc tgcgcgccat accgtggatt tttgcctgga cgcaaaaccg cctgatgctc
     4381 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagatgg caaacagagc
     4441 gagctggaag ccatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
     4501 atggtcttcg ccaaagcaga cctgtggttg gcggaatact atgatcaacg tctggtagac
     4561 aaagcactgt ggccgttagg taaagagtta cgcaacctgc aagaagaaga catcaaagtg
     4621 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
     4681 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
     4741 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
     4801 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
     4861 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
     4921 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
     4981 aggaattgtc gttatattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
     5041 cagaagtcca ggctaaaccc ctttctagct ggaaagccct gggttgggca ctgctctact
     5101 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacca
     5161 acggcattcg cgactcgctg ttattcagct cgctgtggtt gatcccggta ttcctctttc
     5221 cgaagcggat taaaattatt gccgcagtga tcggcgtggt gctatggacg gcctctctgg
     5281 cggcgctgtg ctactacgtc atctacgggc aggagttctc gcagagcgtt ctgtttgtga
     5341 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
     5401 ttgtgcttat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
     5461 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
     5521 ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg
     5581 ataacctagc ctcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
     5641 agtatcgtca gcaactaaac tcgctaacaa agttactgaa tgaaaataat gccttgccgc
     5701 cactggctaa tttcaaagat gaatcgggta acgaaccgcg cactttagtg ctggtgattg
     5761 gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
     5821 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtagttacgt
     5881 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
     5941 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
     6001 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
     6061 cgcgccagac cgacaagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
     6121 acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac
     6181 tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa ataccgctac ccggaaaatc
     6241 agggcaagtt tgatggcaat accgatcatg ttccgccagg attaagcgca gaagagctgg
     6301 aatcatataa cgattatgac aacgctaacc tgtataacga tcatgtggtt gccagcctga
     6361 ttaaagactt taaagcggca gacccgaacg gattcctggt ttacttctct gaccacggtg
     6421 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc
     6481 gccacatgta caccattccg ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc
     6541 cccgtgattt ctcacaggat gtcgatcgta aatacagcct ggcggaactg atccacacct
     6601 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
     6661 cgcagttcaa agaaactacc cgctggattg gtaacccgta caagaaaaac gcgctgatcg
     6721 attacgacac tctgccgtat ggcgatcagg tgggtaatca gtagatgacc tttgtatcgc
     6781 gtctggcgct atttgccgga tgcgatactg ccgcaatgtt tacatcgaac tcccctcagg
     6841 ccacactttt caggtggcct tttttaataa tgccagttac tgttttcccg tctacatacc
     6901 taagggaata acatgtatca cgacgtcagc tatctgctct cccgcctgat caacggcccg
     6961 ctgtccctgc gccagattta ctttgccagc agtaacggcc cggtgcccga tcttgcgtat
     7021 caggtcgatt ttccacggct ggaaattgtg ctggaaggtg agtttgttga taccggcgct
     7081 ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg ctggtggctg gaatttccca
     7141 caatggaaaa cacccgctac cacctttagc gtgctgtttg gcaaacagca actcggcttc
     7201 agcgtcgtgc aatgggatgg caaacaatat caaaatctgg cgaagcaaca cgtcgcccga
     7261 cgcggcccac gcataggttc ttttctgcta caaacgctca atgaaatgca aatgcagccg
     7321 caggagcagc aaacggcaag gcttatcgta gccagcctgc ttagccactg ccgcgatttg
     7381 ctcggcagcc aaatccagac cgcctcacgc agccaggcac tatttgaagc tattcgcgat
     7441 tatatcgacg aacgctatgc ctccgcgctt acccgcgaat ctgtcgcaca ggcattctat
     7501 atttcaccaa actatctctc gcacctgttt caaaaaacgg gggccattgg ttttaacgaa
     7561 tacctgaatc acacgcgact ggagcacgct aagacgttat tgaaaggcta tgacctgaag
     7621 gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca actacttctg ccgtctgttt
     7681 cgtaaaaaca ccgaacactc gccgtcggaa tatcgccggc agtaccacag ccaactgaca
     7741 gaaaaaccga ctactccaga ataagatggg tttgctgcgg cgcagaaagc actttgcgca
     7801 ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta gatgctgcat tgcacatagc
     7861 ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc cgtaatcaac agagcaacat
     7921 ccgcccgacg gatatcctcg tccgctaaac gattctccgt tcccagcgcg ccctgagttt
     7981 caatgctgac gccccacttc tctaactggc acaacttttc cagccgttcc gccgccatat
     8041 aagtatgcgc cacgccgctt acgcaggcgg ttactgccac caggtatgcc attttaacct
     8101 cccacggtaa cctgaaatcc ggcccgttct gccatttcgc gcatcgttgt cacatcggct
     8161 gacgacggcg caggcacctc tttcatcgac catgttttcc ccagcaggcg gtatttcggt
     8221 tcgccgtact gatgaaacgg taacagatgg atctgcctga ttttcagcgg gatcagtaca
     8281 tccagcgcct gctgcatatt ctcccggctg agcgtgaaac cagggatcaa cggtaaacgc
     8341 gggatcacgt tgacaccctc actcaccagc aaacgcagat tctccagcac gcgtggcagg
     8401 ttcatcttca ccacatcccg cgcctgagtc gcgtccataa tttttaaatc gaacagcact
     8461 tcatcgcaca atttcgccag cggtaacagc ttgcttgctg gcgcgtctcc ggcagtttca
     8521 atggcgcatg agacacccca cagccgcagt cgctgtaaaa aacgggtagc aaactccgcc
     8581 tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc cggacgtgcg aaagaaaatg
     8641 tcatctttca tcacttcccg ttccagagcg tcaaggctga tatcgcgacc aatccgttca
     8701 aacgccccgg aggggcattc atccgcatca cgcaaacatt tcgcacagtg cagacatttc
     8761 gcctctctgc gtaccgtctg gattttgccg gagatcgact ccggattagc acaccacggg
     8821 caaagatgcg gacagccttt aaaaaagacc accgtacgaa tgccctcgcc gtcattcagt
     8881 gaataacgct gaatgttgaa aatgcgcgcc acatcatcgc ggcgcgtttc cacaacgtta
     8941 cagctgatgc gctgtccggc ggatgatgtc atcctggatc tccttcgaca gttcgacaaa
     9001 gaaggcgctg tatccggcaa cgcgcaccac cagcccggca taatcttgtg ggcgctgttg
     9061 cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga atatgctgca acttaagctg
     9121 ggtaaacacc cgtaagaagt cggccagttt gcgtaatccc gcttcacctt ccagggtcgc
     9181 cggagtgaat ttcacgttca gcaacgtacc gttagacagc agcgtattat cgagcttact
     9241 gactgacttc agtaccgccg ttggcccttg tgcgtcctga cccagcatag gtgacaagcc
     9301 gccgtctgcc agttgttccc cggcaaaacg accgtctggc gtcgcgccaa ccaccgatcc
     9361 caacggaacg tgagcagaaa cggtatacga tcccggcgtg aagtaaccgc cgcgcgggtt
     9421 ctggtatttt tccacttctt tgcagtagtg gcgcaacagt tcggcgctaa tgttatccac
     9481 ctcgtcgata tcgttaccgt atttctcaaa gcggttaatt aagcgagcgc ggactttttc
     9541 gccttctggc gttgcaaagt tggcttttaa taccgacagc aattcgtcaa aacttaaacg
     9601 ctgttgctca aaaaccatcc ctttcaacgc atggagagaa tcgctcaggt tggcgatacc
     9661 aattccctgt acgccggaga agttataacg cgcgccgcca tcggtaatat cgcgaccttt
     9721 ttccagacaa tcgctgataa aagacgagag cagcggtaca ggtgcccagt cgcgatggcc
     9781 gatatcacaa atattgctgc cctcaaccat cagggtgatg tagtggctga tcttggcgcg
     9841 gatctgctcc agtaaacctt cataagtcag cgcagcattg ccttcatttt catgcaggca
     9901 gatttccatc actttcagca gattaaacat cgcgatgtca tgcaagccgt aggttctgcc
     9961 gggaatagat aattccacac agcccactac ggaatagtcg cgcgcatctt ccagcgaaac
    10021 gccacggttg aggaacgctg gcaccaccac ttcatcgtta aagatttgcg gaataccggt
    10081 gccgaggcga atggtttcgg cggttttcat caggaacggc gtgtcgatca aggcgttagt
    10141 gcgcacgccg aggttcggtt gcggtaattg cacgctttga taggcgtcaa ggcaaaggaa
    10201 cgaaagcacg ttcaccgcgc tacgtccgtt ctcggttaac ccgccgagca gtgcggtata
    10261 gccggtcggg aaaccggcga aataacgcgc gctactggtg gagcgcaaca gcacgatgtc
    10321 gttgcatttc acccataaag attcgagcag ttctttcagg aacgccggat cttcgccctg
    10381 ggttaaagat gcctgataga acggcaacat atactggtcg aagcgcccta aagacagcga
    10441 actggcgttg gattcgtatt gcagaatgat gttcatgtac cagaataact ggcacgcctg
    10501 ccagaacgtc tgcggcttat gttgcgcgtt atggcgggag atctccgcaa tagtcagcag
    10561 ctcttcgcga cgctgggcat ctgtgcagtt tgccgccatc gtttccgcca gttcggcgta
    10621 acgcaaaatg tgtttctgcg aggcttccag cagtaacaac gcggcctgat aaaagtgatt
    10681 ctccggctgt tgctgacaat gttgctgcat ctgtgccacc agctcaccca gaccgtgatt
    10741 caacaggcgt gggtaatcaa taataatgtg cccctggcct ttatccgtct ggttgatgct
    10801 gaaaatctgc gtgttggtcg cggcttttac ttcatctgtc atctgcccgt tgatgaaatc
    10861 tttcatcgaa cgtttttccc agtacgggaa caactcttca cggtagatac gtttatcttc
    10921 ttcgctgata gcaaagcggt cctgcggacg cgtcgggaat tgatccagct ctttcagcag
    10981 ccagtaaggg tccatttccg gcgacataat cccggcgcgc ggttttacgg tgcggttacc
    11041 ggcaatcagt tcttcatcac gaatcgaaat ttcaacatgt tcaaggatat acgctgttgc
    11101 tttcgcccgg cgcaatatca ccggttcgcc ttcggtttgc cgatggctgg cggtataaag
    11161 cagcgcccgc tccagcgaga tttcacgggt attggcaaac agtgcagttt tgaggcgaga
    11221 gatacgattc gtcatgagga actcctgtgg gaaagcgaac ccgcccttgc gagcgggaga
    11281 agaacttaag cggtttgcgc caggtgagct tcaattttgc tcataatggc gtcagcacgt
    11341 tttactgcat cgctgatgtt gacgcgcacg atggtcttgc cagcaaaacg ctcttcaaac
    11401 ttgatgccga tatctttggt cagaatcacc atatcagcgc tcgccacgtc ttccgcagtc
    11461 agttcatttt ccagaccaat tgacccctgg gtttctactt tcacttccca gcctttcgct
    11521 ttcgcggcac tttccagcgc ctctgctgcc atataggtat gggcaacacc tgaaggacat
    11581 gcggttactg caataatttt cgtcatggct cgttcctcaa ttaattaatt tcaaaatcca
    11641 aatccaggtc gtcttctttt tcatcagtcg aagacccatt tttacgcgcc agacttttca
    11701 gcacgttcac acaaacagcc gtcaccactg ctccgacagc cactgctgcg atataaccca
    11761 acttaccttc aaccaccggc agcacaatca gaccacccca gcctgcatag cactgcgcac
    11821 cgaccagcgc cgcagttact gcaccacaaa ctgaaccgac cataatggaa ggaatgacac
    11881 gcagcggatc ggcagcggcg aaaggaatcg ccccttcagt aacgccaaca cagcccatca
    11941 ccagtgccgc tttgccggtt tcgcgctctt ctgcggagaa atttttacga ccaatcagcg
    12001 tcgccagccc catccccagc ggcgggatac aaatacccac ggcagcaatg gccaccacgg
    12061 tataaacacc ctgagcaacg caaatcagca tgaaggcata ggccactttg ttaaccggac
    12121 cgcccatatc gaacgccagc atcagcccca tgatcaccgc cagcataaca atgctgccct
    12181 gctgcatccc ctgaagccac tgagtcaggc tgttggtcaa cgcccctaca ggctcgccca
    12241 agccccacat catgatgcct gcggtaatca gtgtgccgat gataggaata atgaagatgg
    12301 gcatcaccga gcgcagaact ttatgcaccg gaattttctt caggtaatgc accacgatgc
    12361 cgccgataat cccggcgatc agcgcaccaa agaagcccgc accaaagctg ttaccaaccc
    12421 aggcaccgat agcgcacgga gccagcgcag aacgctctgc aatggagtaa ccaatgtaag
    12481 cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc gatatcaaac agttttttca
    12541 aattcggatc ggctacggca tccggcactg cgcctttgcc atacaacatg acggaaaccg
    12601 ccagcaaaat accgcccgat accacgaagg gaatcatgtg tgaaacgccc gtcattaaat
    12661 gctgacgggt atttttcagg atctgcacca actcattcat aagctgctcc cgggcttgca
    12721 aaagcccatg tccatatttt tgatggagga aacaataggc aatcgcaggc ggagcaaaca
    12781 gtggataaaa aggccattta ctggactttt gtaatatcag tacaaaaatg cgatccgcct
    12841 cataacttgc gataatccgg aagcatgccg caaaacggcg tcttgtgaga cgcgtcgcaa
    12901 attgacggtt acattacatt tgtccagctt ttggcagatt tgtcacatgg caggccgtta
    12961 ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga gaaaggcaat ggccctgatt
    13021 gtggaattta tttgtgagct acctaacggc gtacatgcgc gtccggcaag ccacgttgaa
    13081 acgctgtgta atactttttc atctcaaatt gagtggcata acctgcgtac tgaccgtaaa
    13141 ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata cgctggtggg tgataactgc
    13201 cagctactga tttccggggc cgacgaacag gaagggcacc agcgtttaag ccaatggctg
    13261 cgcgatgaat tcccccactg cgatgcgccg ctggcggaag ttaaatctga cgaactggaa
    13321 ccactgccgg tttcactgac caatctgaat ccgcaaatta tccgcgcccg caccgtgtgc
    13381 agcggtagtg caggcggcat tctgacgccg atctcttctt tagatctcaa tgcgctgagt
    13441 aatcttcccg cagccaaaga cgttgacgcc gagcaatccg cactggaaaa cggcctgacg
    13501 ctggtactga aaaacattga gtttcgtctg ctggatagcg acggtgctac cagcgcgatt
    13561 ctggaagcgc accgatccct ggctggcgat acttccctgc acgaacattt actggcaggc
    13621 gtcagcgccg gattaagctg cgccgaagcg attgtcgcca gtgcgaatca cttttgcgaa
    13681 gagtttgccc gttccagcag cagatacctg caagaacgtg ccctggacgt acgcgacgtc
    13741 tgcttccagt tactccagca aatctacggt gagcaacgct tcccggcacc gggcaaactg
    13801 acgcagtccg ccatttgtat ggctgatgaa ctgaccccca gccagttcct cgaactggat
    13861 aaaaatcacc tcaaaggatt gttactgaaa agcggcggca ccacctcaca tacggtgatc
    13921 cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg atattgatgc cctcactccg
    13981 tggcagcatc aaacgattta tatcgacggc aacgccgggg cgattgtggt tgagccagga
    14041 gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac aggacgccct gcgtgagcaa
    14101 cagcgtgtct agctgaccca acaagcccgt accgctgacg gtatccgcat tgaaattgcc
    14161 gctaacatcg ctcactccgt ggaagcgcag gccgcattcg gcaatggtgc ggaaggcgtt
    14221 ggtttgttcc gcactgaaat gctctatatg gatcgcacca gtgcaccggg cgaaagcgag
    14281 ctgtacaaca ttttttgtca ggcgctggag tctgccaacg gacgcagcat tattgtgcgc
    14341 actatggaca ttggcggcga caaacccgtt gattatctaa acattcccgc agaggcaaac
    14401 ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat acgcgtcgtt atttaccaca
    14461 caactacgct cgatcctgcg cgcctccgct cacggcagcc tgaaaatcat gatcccgatg
    14521 atctcctcaa tggaagagat cttatgggtg aaagaaaaac tggcagaagc caaacagcaa
    14581 ctacgtaacg aacacattcc gtttgatgag aagatccagc tcggcattat gctggaagtg
    14641 ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga ttgatttctt tagtattggt
    14701 agtaatgacc tgacgcaata tctgctggca gtggatcgcg ataacgctaa ggttactcgt
    14761 cactacaaca gcctgaatcc ggcattcttg cgggcgctcg attacgacgt gcaggcggtg
    14821 catcgccagg gtaaatggat tggtctgtgc ggtgagctgg gagcgaaagg ttccgtgctg
    14881 ccgttgctgg tcggcttagg gctggatgaa ctcagcatga gcgcaccatc aattccggcg
    14941 gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc gcaagttgct caaccaggca
    15001 atggcctgcc gtacttcgct ggaagtggaa cacctgctgg cgcaattccg catgacccaa
    15061 caggacgcac cgctggtcac cgccgagtgc atcacgctgg aaagtgactg gcgcagcaaa
    15121 gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg cgggccgctg ccgctatccg
    15181 cgtaaactgg aagccgactt atgggcgcgc gaggccgttt tctctaccgg tctgggcttt
    15241 agttttgcca ttccacacag caaatcagaa cacattgagc aatccaccat cagcgtggcg
    15301 cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc aattcatcat tatgttaacc
    15361 ctgaacaaac acgctgcggg cgatcagcac atgcgcattt tctcgcgcct cgctcgccgc
    15421 atcatgcacg aagaattccg taacgcgctg gttaacgccg cctctgccga cgctatcgcc
    15481 agcctgctgc aacatgaact ggaactgtaa aaggaaacat catggaactg tatctggaca
    15541 ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt cccgattgcc ggggtgacaa
    15601 ctaacccgag cattatcgct gccagcaagg agtccatctg ggaagtgctg ccgcgtctgc
    15661 aaaaagcgat tggtgatgag ggcattctgt ttgctcagac catgagccgc gacgcgcagg
    15721 ggatggtgga agaagcgaag cgcctgcgcg acgctattcc gggtattgtg gtgaaaatcc
    15781 cggtgacttc cgaaggtctg gcagcaatta aaatactgaa aaaatagggt attactacac
    15841 ttggcaccgc tgtatatagc gccgcagaag ggttattagc cgcactggca ggggcaaaat
    15901 acgttgctcc gtatgttaac cgcgtagatg cccagggcgg ggacggcatt cgtacggttc
    15961 aggagctgca aacgctgtta gaaatgcacg cgccagaaag catggtgctg gcagccagct
    16021 ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg atgtgaatcc atcaccctgc
    16081 ccttagatgt agcgcaacaa atgctcaaca cccctgcggt agagtcagct atagagaagt
    16141 tcgaacacga ctggaatgcc gcatttggca ctactcatct ctaaaggagc aattatggac
    16201 cgcattattc aatcaccggg taaatacatc cagggcgctg atgtgattaa tcgtctgggc
    16261 gaatacctga agccgctggc agaacgctgg ttagtggtgg gtgacaaatt tgttttagtt
    16321 tttgctcaat ccactgtcga gaaaagcttt aaagatgctg gactggtagt agaaattacg
    16381 ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc tgcgtggcat cgcggagact
    16441 gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa ccctcgatac tgccaaagca
    16501 ctggcacatt tcatgggtgt tccggtagcg atcgcaccga ctatcgcctc gaccgatgca
    16561 ccgtgcagcg cattgtctgt tatctacacc gatgagggtg agtttgaccg ctatctgctg
    16621 ttgccaaata acccgaatat ggtcattgtc gacaccaaaa tcgtcgcggg cgcacctgca
    16681 cgtctgttag cagcgggtat cggcgatgcg ctggcaacct ggtttgaagc gcgtgcttgc
    16741 tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca cccaggcggc gctggcactg
    16801 gctgaactgt gctacaacac cctgctggaa gaaggcgaaa aagcgatgct tgccgccgaa
    16861 cagcatgtag taaccccggc gctggagcgc gtgattgaag cgaacaccta tttgagcggc
    16921 gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt gcataacggc ctgaccgcta
    16981 tcccggacgc gcatcactat tatcacggtg aaaagtggca tttggtacgc tgacgcagct
    17041 ggtactggaa aacgcgccgg tggaggaaat cgaaaccgta gctgcactta gccatgcggt
    17101 aggtttgcca ataactctcg ctcaactgga tattaaagaa gatgtcccgg cgaaaatgcg
    17161 cattgttgca aaagcatctt gtgcagaagg tgaaaccatc cacaacatgc ctggcggcgc
    17221 gacgccagat caggtttacg ccgcactgct ggtagctgac cagtacggtc agcgtttcct
    17281 gcaagagtgg gaataaccta ctccaaactc ccggcttgtc cgggagtttg aacgcaaaat
    17341 tgcctgatgc gctacgctta tcaggcctac gcaatctctg caatatattg aatttgcgtg
    17401 cttttgtagg ccggataagg cgttcactcc gcatccggca ctctgtgcca gtaatctgaa
    17461 actcccacgt tgttcaggag ttttttatcc gcttctggac attttctcca cagaacaatc
    17521 gttagcccgg aaggtcgaat ctgtgactct tattttcacg gacgaaccag atgaaagctt
    17581 cactggcgct gctcagcctg ctaacggcat ttaccagcca ctccttaaaa tctcccgccg
    17641 ttccaccaac cgtggtacaa atccaggcca ataccaacct tgccatcgca gacggtgcca
    17701 gacagcaaat tggcagcacg ctattttacg atcgcagctt acctatcctg gcggtgatgt
    17761 tccgcaagaa cgcggtgtat gttccgatgt ggtgatccgc gcattgcgca gccagaaggt
    17821 cgatttgcag aaactggttc acgaagatat ggcgaagaat tttgctgagt acccgcaaaa
    17881 atggaagtta aagcgcccgg acagcaacat cgatcaccgt cgggtgccta atctggaaac
    17941 ctggtttagc cgccacaaca aaacacgccc catcagtaaa aatgccggtg actatcaagc
    18001 gggcgatatt gtctcctggc gattagataa cgggctggcg catattgggg tggcgtcaga
    18061 tggcttcgcc cgcgacggta cgccgctggt gatacataac attggcgcag gcgcgcagga
    18121 agaagatgtg ctgtttagct ggcggatggt ggggcattat cgttattttg ttaaataaga
    18181 gatcgcccca catgaagaaa aaattcagat gctttttaca ctgacagctt cttttttctt
    18241 acggctcacc agcgccagtg ccagcacaat cagcacaata ccgctacctt caacggcccc
    18301 cggattctca ccgagcagcc accaggaaaa gagtacgcca caaacgggaa cggccagtgt
    18361 gcttaagctg gcaatactgg caggcaagtt tttcagcaca aacagccata agctccacgc
    18421 tagcgccgtc gccagaatcg cactgtaggc cagcgcccag aacacggtgg gctgccagtc
    18481 aatttcacgt tgcggcacca gtaaagcgac cacactcatc accagcgccg catacagcat
    18541 ctgccaggat gttaacgata ataaatccac gcgcggatga cgggcgtata ggcgtttagc
    18601 aacaatcgcg ctcgcccccc aactaacacc ggagaggatt gccagcatgg cacttttcat
    18661 cgaagagaaa tcgagttgcc acggctgcaa caccaaaaat aagccgaaag cggcaatcag
    18721 aatcgcgaaa tattgcccac gtcgcaggcg ttcaccgaga aacaacgcgg cgaaaatcac
    18781 cacccagaac ggcatggtat agctcaggat cgccaccttc cccgcacctc cgctgaccaa
    18841 cgcccactgc gccagaccaa ccatcccgca ggtttgtaac agggcaatgg ctaaggtgta
    18901 tttaaacggc gtcgggcgca ttccgcgacc acgtaataaa aggacgatga ataaaacgag
    18961 agcgccgaaa atgcagcgta aggcggtaaa gtcgaaggca ccgatgtaac tggtgacttg
    19021 cttcatgaaa atccagctat aactccagat aagtgtgagc acaaccaagc cactgattgc
    19081 cagtgggttg ctctttcctg cggcagacat gagagatcca gtgtgtagtt ttcctgtgga
    19141 cactatacgc cggtaaagaa acttcagcga ctgcctgaaa gacagtcgct gaagaaggtc
    19201 agattacagc aggtcgaaac ggtcgaggtt catcactttc acccatgccg ctacgaagtc
    19261 tttaacaaac ttctcgtggg catcgctgct ggcgtaaact tccgctactg cacgcaggac
    19321 agagttagaa ccaaatacca gatccgcacg gctggctgtg tatttcactt cgccggtttc
    19381 gcggtcacgt ccttcgaaca gctctttcga ttcgtcagtc gctttccact cgtaacgcat
    19441 atccagcaag ttcacgaaga agtcattgct caatacgcca acgcggtcag tgaagacgcc
    19501 gtttttgctg ccatcgaagt tggcacccag tacacgcatc ccgcccacca gcgcagtcat
    19561 ttccggcgcg gtcagcgtca gttgctgtgc tttgtcgatc agcagtgact cggtggtgga
    19621 aacgtccaga cgagcgcgat agttacggaa accgtcagca attggctcca gcagctcaaa
    19681 catctcaatg tcagtctgat cctgacgcgc atcaacgcga cccggcgcaa acggtacatg
    19741 aatgctcaaa cctgcggcgc ttgcggcttt ctcaacacca accacaccag ccagcacgat
    19801 gatatccgcc agcgaggctt taccagactc tttctggatt ttctccagaa caggcagagc
    19861 acgaacggct gcggcgttca catcccagtc gcgctgcggc attaatgcca gacgcgcacc
    19921 gttggcacca ccgcgtttgt cgccaccacg gaaggtagaa gcagatgccc aggccaccga
    19981 taccagctca ctaacagaca gaccagaatc cgcaatcgcg aatttcagat cgataatgtc
    20041 ctgctcggtc gggttgtaga tcggctgcgg cagcggatct tgccagatca gatcttcttt
    20101 cggcacttcc ggcccgatgt agcgagattt cggccccata tccctgtgcg tcagtttgaa
    20161 ccaggcacgg gcaaaggctt cgttgaacgc ctgcggatcg ttgaggaaac gacgagagat
    20221 cttctcgaac tcaggatcaa aacgcagcgt caggtcggtc accagcattg tcggtttacg
    20281 tttcttcgac ggatcgaacg gatccgggat aatttccggt gcgtctaccg cttcgaactg
    20341 gattgctcca gccggactgc gggtctgtac ccactcatac ttgaacagat tctcgaagaa
    20401 atagttgctc cactggatcg gcgtctgggt ccagactact tccagaccag aggtaatggc
    20461 atctgcgcca acgccgctgc cgtaagtgct cgcccagcct aaaccttgtt cttcaatcgg
    20521 tgcagcttct ggatcaggac ctacgtttga tgtcggaccg gcaccgtggg ttttacccag
    20581 cgtatgacca cccgcaatca gcgccacggt ttcttcgtcg ttcatgccca tgttgccgaa
    20641 ggtcgcgcgg atagctgctg ccgcagaaag cggttcgccg ctgtgatccg ggccttccgg
    20701 gttaacgtaa atcaggccca tctcggttgc acccagcggt gctttcgcca gcgcttccgg
    20761 atgacggtga gtcagccagg ctttttcatc accccagtta acatccagat ccggttccca
    20821 gacgtcttca cgaccggcac caaaaccgaa ggtacggaag ccggagtttt ccagcgccac
    20881 gttacctgcg aggataaaca ggtcggccca ggagattttc tgaccatatt tctgtttgat
    20941 tggccacaac aggcgacgcg ctttatcgag gcttacgtta tccggccagg agttcagcgg
    21001 tgcaaaacgt tgctgaccac gacccgcgcc gccgcgtcca tcgattgaac ggtaagtacc
    21061 tgcgccgtgc caagccatac gaataaacag accggcgtag ctgccccagt cggctggcca
    21121 ccacggctga gattctgtca gcagggcttt cagatctttt ttcaggccgt agtaatctaa
    21181 tttgctgaat tctttgcggt agtcaaagtc ctcacccagc gggttagaac gattagaatg
    21241 ttggtttaac aggtcaacac ggagttgatt tggccaccag tcgcgagtgg ttgtgcccgc
    21301 ccccgcgctc tggtcgtgac cgccctgatg gaacgggcat ttgccagtgg ctgtggtgtt
    21361 atggatatcg tctgacgtgc tcatcaatgt gctcccctct acagtgttac cgttacgata
    21421 cacagcgtta gagagaagtt ataattgaat taatccacgg atgcgatagt tgagatctta
    21481 catattgttg gttaaagaga tgtagatcaa attgatcgta attaagcttt tttgattatg
    21541 tccgaattcg gacaaatgct ttataaaaaa gggttttagc ttcatatcct tcaagtaatg
    21601 agggagaaaa caaggctgac aaccttattt tcccgccctc atttcgaggc agcattttgt
    21661 gctctgttta aaatttgtga tcactgtgtg attttcacaa aagccacact atttataaac
    21721 caggtcgaac acccagcgta tggcaaatcg cgtaactcat ttcagcgcgg ttaagcgtat
    21781 agaagtggaa atctttcact ccttcacggc ttaaaatctt caccatatcc atggcaatat
    21841 tcgcgcccac cagtttgcgg gtttcggcat cgtcatccag cccgtcaaac atttgcgcca
    21901 tccacgccgg aatacgcacg ttggtcatgt cggcaaattt cttcgcctgt ttaaagttag
    21961 ataccggcaa aatgcccggg atgatttcca catcaatgcc cgccgataca cagcggtcac
    22021 gaaaacgcag gtagctttcg acatcgaaga agaactgagt aatcgcgcgg ttggctccgg
    22081 catccacttt gcgtttcaga ttaagcaaat ccgcctgagc gctttttgct tccgggtgaa
    22141 cttccggata cgccgccacg gatatatcga aatctgccac ttcttttaac agcgtcacca
    22201 ggtcagaagc atacatttct ggcttaccac ttcccggcgg cagatcgcca cgcagtgcca
    22261 cgatatgacg aataccgtta ttccagtagt cgcgtgcaat ggtgcgcagc tcgtcgggcg
    22321 tcgcatcaat gcaagtaaga tgcggtgccg cttccagacc agtgcgatct ttaatgcctt
    22381 taataatgct gtgcgtacgg tcgcgctcgc cggagttcgc gccataggtc accgatacaa
    22441 acttcggttt caggctgcta aggcgatcga tggagttcca cagggtctgc tccatttcac
    22501 tggtacgcgg cgggaaaaac tcgaacgaaa cgttaatctg cccctggact tctgccaggc
    22561 tctgattcag ggcatcccgc tggctggcgt gaaaaaagct cataccttac ctcatcaatc
    22621 gcttgtagtt atatgttgtg ttgtgcacat ctatccattt agacgtccag atgtaaagat
    22681 gaaggaaaag ccgaagggcg tcaaccgaaa aatgaccaat gatggtgagg aaaaatcagg
    22741 gaagatgaaa aaacttcagg atggtaagaa aaagaaaatc cgatatgcaa agcaaaccgg
    22801 actgcaaata taaactaacc atgtacttat tgcttcttgc cggattcgtc gtaaacgctt
    22861 catccggcct aattctatga taattcaata tatttcatta gaaaccatag acccgaaaac
    22921 atgagtaccg ggcattatta aatttctgaa attacaacaa ctgtgccagc cggttgatat
    22981 ccgactgaat cgccccggcg gtgacgtcgc gcccagcgcc aggcccacgg atcaccagcg
    23041 gattatcgcg gtaccagcgg ctttcgatgg caaagacgtt atcgcatggc agcagtgatg
    23101 ccagcggata gtcttcacgc accgcctcca cgcccacacg cgctttgccg ttggcatcga
    23161 aacgcgccac gtagcgcagc accagcccca tttcgcgggc cgcttccagc cgttgcacca
    23221 tctgctcgtt aagttcatcg ccattttcaa agaaatggtc gatgctgccg ccttcgcaat
    23281 gagcaggcac cagcgattcc acacgtacct ggtccggttc aatgttgtaa cccgcttcac
    23341 gcgccagaat caccagcttg cgcattacgt ctttgccgga gaggtcgtca cgcggatcag
    23401 gctcggttaa gccctgctgc cacgcctgat ccaccagctc ggtaaacggc acgctaccgt
    23461 cgaattgcag gaacagccag gagagcgtgc cggagaagat cccactgatc gacaaaatgg
    23521 tatcgccgct gtcgatcaga tcgcgcacgg tatggttgat cggcaagccc gcaccaacgg
    23581 tggcgttgta tagccagtga cgcccggttt tttcgaaggc gtcgtggatc tggcgatatt
    23641 tattgctgtc gcttgctccc gccagtttgt tggcactgat gacgtggaaa ccgtggctgg
    23701 cgaaatcgag atactgatca gcaagctgct ggctggcggt aacgtccaac accactaaat
    23761 catcatacgg atgggcgcgc atccacagga aaagcgactc ttcatcctgc tcgaccgctt
    23821 catcgttgaa gaaggctaac gcgcggctgg cgtccagccc gtcataactc aacagactgc
    23881 ggcggctgtc caccacgcct gccagcacaa attcaaagcc ggtacgcgcc gaaagcgtgc
    23941 tctgctcgcg ggcgaacagt tccagccagc gagagccgat attgcctttg ccgaacaata
    24001 ccaggccgat gcgtttttct gcgcggaaga cggactgatg cagcccctga atcaggcttt
    24061 cggtcgggcc ggtgcgcagt actgccacca gactgatgcc gtcatcggac tgccacgtaa
    24121 actcgaccgg ctggcctttc agttgctgcc agaagcggtg gcaatgcagc gggttacggg
    24181 tgacacccgc cccgaccatc gccaccagcg ccagcccctg acgcaggcgc agttctccag
    24241 gtaatcccgc ttcgtcgagg attttcagcg cactgtcggc cacttctgag gtgtagcaaa
    24301 attgcaacaa ctggcgatcg ttatgcacgc caaccgccag tggacgcacc tgggcacgtt
    24361 tcaggatctg gtcgatctct ttatgcgcca gtttgaaatc ctgactggcg ggcacctgaa
    24421 actcaatcaa acagacatca tcgtggctgg tgacaatacg cgcaccagta ccggaggcca
    24481 gcacgcgttc aatgcgcgtg gaaccttgat ccggcgtgta gctacagcgc agctgcaggt
    24541 cgatttcgct gccagaaact ggctgtaaag tacgggcgtg aagaacggga gccgccagac
    24601 gcgccagttc gctggcctca tccagacgca gcaacggcag caggcaggca tctttcactt
    24661 tacgcgggtc ggcactgtat accccggcga cgtcgctcca gatggttaca cgagaaacac
    24721 ccgccagcgc accgatttgt gtcgcggaat agtcggaacc gttacgcccc agcaacaccg
    24781 tttcaccggc gttgttacgg ctgataaacc cggtcaccac cagacgtttg ctcggatgtt
    24841 gtaccagtag ctgttgcagc aacgggtaag aaagcccttc atcaacctgc ggttgcgcag
    24901 cgcgttcagc gcgtaaaaac tcacgggcat caagccaggc cgctggcagc ccttgttgat
    24961 taagtaccgc agacatcagg cgcgccgacc acacttcacc gtggcccacc acttccgcat
    25021 acaccgcatc gttaatacca ctgtcgagca acgccgccag gcgctcaaga tcgctgacaa
    25081 aagcgctaat gaggctatcg gcttcttcag cgggtagcag accgctaatc agatcgcact
    25141 gatagcgacg cagcgtttgt tgaacctgat gcgcagagag acgatcggtc tggcttagtt
    25201 tcaaccagtt aatcaactgg ttagtggtgc taccggcggc ggaaaccacc atcatatcgt
    25261 caggctgaga gaactccgcc ataatgcccg cgacacgcaa ataacacttc acatcagcca
    25321 gactactgcc accaaattta tgcagctgac gacctttcgc ccctgcctgc gcaatcacac
    25381 tcatttttac cccttgtttg cagcccggaa gccattttcc aggtcggcaa ttaaatcttc
    25441 gccatcttca ataccggtgg agatacgcag cagcgtctcg gagatcccgg cggcagcacg
    25501 cgcttctggt gccatgcctg catgtgtcat ggttgcggcg tgagagatta aactttccac
    25561 tccccctaat gattccgcca gcgtaaacaa cgacagcccg cccaggaaac gacacagcgt
    25621 ctgctcatcg ccatccagtt caaaactcaa cattgcgcca aagccttttt gctgacgcgc
    25681 ggcaatttca tgcccctgat tttccggcag cgacgggtga tacagttttt tcaccaacgg
    25741 ctgggtttgc agatatttca caatcgcctg cgcgttgcgc tgcgccagct ccatacgcgg
    25801 caccagcgtt cgcaacccgc gtagcagcag atagctgtca aacgcgccgc ccgtcacgcc
    25861 aatattgttt gcccaccagg caagttcagt gacaacgtcc gggtctttag caatcaccac
    25921 gccagccacc acgtcagagt gaccgttcag atatttcgta catgaatgca acaccagatc
    25981 ggcacccaat gccagcggat tttgtaatgc cgggcttaag aaggtgttat ccaccacgct
    26041 caccgccccg acttcccttg ccagatggca gattttcgca atatccacga cgcgtaacaa
    26101 tggattactt gggctttcta ccagtaccag tttgggtttt tctgccagcg ctgcccgtaa
    26161 tgcctgttca tcgccttgat caacaaacaa cacgcgatag caaccgcgtt tcgccagact
    26221 gtcgaacagg cgatagctac cgccgtagca gtcgtgcggc gcaaccagca gatcgccagg
    26281 tttcaaaaag acggtcgtta ccaggtgaat cgcggacatg ccggtattag taagtactgc
    26341 gccagcaccg ccttccagtt ctgccagcgc acgctgaacc acatcgcgcg ttgggttgcc
    26401 gcgacgcgag taatcatgcg cgcgcggttc attaaatccg gtaaagttat aggtgctgga
    26461 aagatggatg ggagggacaa cgcaaccata ctgttcgtcg tcatttaacc cgctacgcac
    26521 tgcgatggtg gcctgtttac gcgtcatgtg atgaagtttc ctgggctttg tcggtgaaat
    26581 gtcaggcacc agagtaaaca ttgtgttaat ggacgtcaat acatctggac atctaaactt
    26641 ctttgcgtat agattgagca aatcccaaat agccgttaaa attatatgca ttatcacgcc
    26701 gacaggtgca ttacacgatg tcacggtaac gcctgtacgg taaactatgc gggtttacgg
    26761 tcagtaccca cataaattgt ggggtctggt ctcaatttat tgacgaagag gattaagtat
    26821 ctcatggctg aatggagcgg cgaatatatc agcccatacg ctgagcacgg caagaagagt
    26881 gaacaagtca aaaagattac ggtttccatt cctcttaagg tgttaaaaat cctcaccgat
    26941 gaacgcacgc gtcgtcaggt gaacaacctg cgtcacgcta ccaacagcga gctgctgtgc
    27001 gaagcgtttc tgcatgcctt taccgggcaa cctttgccgg atgatgccga tctgcgtaaa
    27061 gagcgcagcg acgaaatccc ggaagcggca aaagagatca tgcgtgagat ggggattaac
    27121 ccggagacgt gggaatacta aagcggaaaa gagcggcgcg gagtggaatc gcctgatgca
    27181 ctacgcttat caggcctacg tcatattgca atttattgaa tttgcacgaa cttgtaggcc
    27241 ggataaggcg ttcacgccgc atccggcata aacaacgagc acgttgtctg cgacccaccg
    27301 ctttttatac atggacgttt aactatgaaa aacaggctgc tgatcctcag cctgctggtt
    27361 tctgtacctg cctttgcctg gcagccgcaa actggcgaca tcatctttca aatctctcgc
    27421 tcatcgcaaa gtaaagcgat ccaactggca acccattccg attacagcca caccggtatg
    27481 ctggtgatgc gtaacaaaaa gccctacgtt tttgaagcgg tcggcccggt gaaatacacc
    27541 ccgctcaagc agtggattgc ccatggtgaa aagggcaact acgttgttcg ccgcgttgaa
    27601 ggcggactga gtgttgaaca acagcaaaaa ctgacgcaaa cggcaaaacg ttatctcggt
    27661 aaaccgtatg atttcagctt ctcatggagc gacgatcgcc agtactgttc ggaagtggtg
    27721 tggaaggttt accagaacgc gctgggaatg cgcgtgggcg aacagcagaa gttgaaagag
    27781 tttgatctca gcaacccgct ggtccaggcg aagctcaaag aacgctacgg taaaaatatt
    27841 ccgcttgagg aaacggtagt ttcaccacaa gccgttttcg acgcgccaca actcaccacg
    27901 gttgccaaag agtggccgct gttttcgtgg tgatacgacc aaaattcagg cacaaaaaaa
    27961 gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg
    28021 cttgttgaag cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa
    28081 cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt
    28141 tattacgtta ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt
    28201 tttcatggga aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc
    28261 acgcgatgtt aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac
    28321 agaaattaac cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac
    28381 ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc
    28441 gcttcctcgt acctttgact atctgctgcc agaaggcatg acggttaaag ctgggtgtcg
    28501 cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg attgtggtat cagttagcga
    28561 tgccagcgaa ctgccgctca ctgagctaaa agcggtagtc gaagtgctgg atggtgagcc
    28621 ggtgtttact cactccgtct ggcgattgct gctatgggcg gcagattact atcatcatcc
    28681 gattggcgat gtgctgtttc atgccttgcc gattttacta cgccaggggc ggcctgcggc
    28741 gaacgcgccg atgtggtact ggtttgccac tgaacaaggt caggcggtgg atctgaacag
    28801 cctgaaacgc tcacccaagc aacaacaggc gctggcagcg ttacggcaag gcaaaatctg
    28861 gcgcgaccag gttgccacgc tcgaatttaa tgatgccgcg ttgcaggcgc tgcgcaaaaa
    28921 aggtctgtgt gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc
    28981 cgtttctggt gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca
    29041 tagcgcggca gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac
    29101 ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat
    29161 ggtgccggaa atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc
    29221 gcccgtggaa gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa
    29281 agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt
    29341 taaaaatctc ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga
    29401 aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc
    29461 gattattctt ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa
    29521 ataccgcctg ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt
    29581 gctggattta aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat
    29641 gcgccagcat ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc
    29701 gcctgcgcta ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta
    29761 ctacacgctg catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc
    29821 ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac
    29881 cgaacagctt gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga
    29941 ccgcgatacc accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg
    30001 cggtgcgagg attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt
    30061 gacgctggtg gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc
    30121 agagcgtttc gctcagcttt acacccaggt cgctggtcgt gccgggcgtg cgggtaaaca
    30181 aggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta
    30241 taaaggctac gatgcctttg ccgaacaggc gctggctgag cgacggatga tgcaactgcc
    30301 gccatggacc agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct
    30361 gttcctgcaa caactgcgta atctgatcct ctccagcccg ctggcagacg acaaactgtg
    30421 ggttctcggt ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat
    30481 attgttgcag cacccttccc gcgtgcgctt gcaacacatc attagtggta cgctggcgct
    30541 catcaataca ataccggatt cccgcaaggt gaaatgggta ctggatgttg atccgattga
    30601 gggttaaacc gcttacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca
    30661 tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa
    30721 acatggatgt agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag
    30781 gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc
    30841 tcccgagcat taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa
    30901 gcggcccggg aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa
    30961 tcccgcacca ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt
    31021 cgcggtatcg aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg
    31081 catcaaaatc agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc
    31141 atgttgctgc tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg
    31201 ccgccgatgg tgatggcgaa cgaatttgca ccggaactgg agctgcctac cgttcatatc
    31261 gacaatctga ccgccgcatt tgatgcagta aattatttat atgagcaagg gcataaacgg
    31321 attggctgta tagccggtcc cgaagagatg ccactgtgtc actaccgcct gcaaggctat
    31381 gttcaggcgc tgcgtcgctg cggaattatg gttgatccac aatacatcgc ccgtggcgac
    31441 ttcaccttcg aagccggaag caaagcaatg caacagctgc ttgatcttcc acaaccgcct
    31501 actgcagtct tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc
    31561 caggggctga aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg
    31621 caattttgtg atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa
    31681 gctatgctgt tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta
    31741 atggactgcg aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta
    31801 agactgccgt gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa
    31861 taaatacagc gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc
    31921 ttcgcggcga aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc
    31981 cgctatggtc gctattgctg ccgccgttct tgtgaccttt atcggtggtc tgtacttcat
    32041 tacgcatcac aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggaaacgg
    32101 actaccacca aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc
    32161 gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact
    32221 gacaccagaa caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac
    32281 gcagctggtt gaggttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca
    32341 acgccagcgt caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac
    32401 gactgaacaa agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca
    32461 accgcgccag tctaaaccag cttctactca gcagccgtac caggatctgc tgcaaacccc
    32521 tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga
    32581 cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc
    32641 gttcagaggc gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga
    32701 ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa
    32761 agagaacgca gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg
    32821 gctcgccgcc gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc
    32881 gtacttttgt acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact
    32941 atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg
    33001 ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc
    33061 atcgcgggct ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt
    33121 aaactggaaa tgcatcaggg ccatctggtt aaagccgccg ttgagctggc aaaagactgg
    33181 cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca
    33241 tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc
    33301 ggctccggcg gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt
    33361 agcgcccgtg aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc
    33421 aaccatttcc acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa
    33481 atgaccccac gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc
    33541 aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag
    33601 ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa
    33661 actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg
    33721 accaaattta ccgaagtggg ctacgtcggt aaggaagttg attctattat tcgcgatctg
    33781 accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct
    33841 gaagaactgg cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg
    33901 ggacagactg aacagcagca ggaaccttcc gctgctcgtc aggcattccg caaaaaactg
    33961 cgtgaaggcc agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc
    34021 gttgaaatta tggctcctcc gggcatggaa gagatgacca gccagctgca gtccatgttc
    34081 cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag
    34141 ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct
    34201 atcgacgctg ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag
    34261 cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg
    34321 ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg
    34381 tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa
    34441 ggtcggctgc caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt
    34501 ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc
    34561 gtaaatatcg agtttaccga ctccggtatt aaacgcatcg cggaagcggc atggcaggtg
    34621 aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg
    34681 gaagagattt cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat
    34741 tatgtgagca aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta
    34801 taatcgcgtt caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg
    34861 gcgcgtatta tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga
    34921 cctaaaaccc tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg
    34981 caaggtcact tcgatccgct ggtcgccctg ctggcactaa ttaccgccgg gctattacag
    35041 atcctttcta acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac
    35101 cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta cccagcaaaa gatgaaacgg
    35161 gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca
    35221 tgccatacgc tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc
    35281 gctatcacct acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc
    35341 gtactggttt tctttggctg gttgagtgtc atggggagct ggtatttaca ggctcataca
    35401 ttgattccgg cactgatcct tccggcgacc gcatgcggcc tgctggcaac ggcagtactg
    35461 aatattaata acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg
    35521 gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctcgctg
    35581 gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg
    35641 ctggcggcac cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg
    35701 gcgatgcgac caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt
    35761 gttttaggga tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg
    35821 atgattttgc caacagccca catagcgcga tatactgaaa attctcgcag caactgaatg
    35881 ttaagcctat gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg
    35941 tcgtggaacc gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca
    36001 cggtaaaatg tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg
    36061 gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac
    36121 tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt catttacggc gcggtgcgtc
    36181 aggtagatga cctggaagag ttggatatcg gcatccaggc gatggcggca attccggttg
    36241 gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtcacct
    36301 tcttctccgg cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc
    36361 tggatattga atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc
    36421 ctacacagta aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc
    36481 agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca
    36541 cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt
    36601 ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt
    36661 acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc
    36721 aaggcgttga tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga
    36781 gcgcacttta gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct
    36841 tgcagattac ggtttgccac acttttcatc cttctcctag tgacataatc cacatcaatc
    36901 gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag
    36961 atggaataaa tggcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga
    37021 tatttctcgt ttttgctcgt taacgataag ttaacagcat gcctacaagc atcgtggagg
    37081 tccgtgactt tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac
    37141 atcaaccttg aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt
    37201 cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat
    37261 cagtgtcatt tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg
    37321 cgcgcatctt aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg
    37381 caaagttatt ccttttatcg tttcacaagt tgccggcgct ttctgcgctg cggctttagt
    37441 ttacgggctt tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg
    37501 cagcgttgaa agtgttgatc tggctggcac tttctctact taccctaatc ctcatatcaa
    37561 ttttgtgcag gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct
    37621 ggcgttaacg gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg
    37681 tctactgatt gcggtcattg gcgcatctat gggcccattg acaggttttg ccatgaaccc
    37741 agcgcgtgac ttcggtccga aagtctttgc ctagctggcg ggctggggca atgtcgcctt
    37801 taccggcggc agagacattc cttacttcct ggtgccgctt ttcagcccta tcgttggcgc
    37861 gattgtaggt gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg
    37921 tgttgtggaa gaaaaggaaa ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga
    37981 ctacgggaca attaaacatg actgaaaaaa atatatcgtt gcgctcgacc agggcaccac
    38041 cagctcccgc gcggtcgtaa tggatcacga tgccaatatc attagcgtgt cgcagcgcga
    38101 atttgagcaa atctacccaa aaccaggttg ggtagaacac gacccaatgg aaatctgggc
    38161 cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc gatatcagtt ccgatcaaat
    38221 tgcagctatc ggtattacga accagcgtga aaccactatt gtctgggaaa aagaaaccgg
    38281 caagcctatc tataacgcca ttgtctggca gtgccgtcgt accgcagaaa tctgcgagca
    38341 tttaaaacgt gacggtttag aagattatat ccgcagcaat accggtctgg tgattgaccc
    38401 gtacttttct ggcaccaaag tgaagtggat cctcgaccat gtggaaggct ctcacgagcg
    38461 tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg tggcttatct ggaaaatgac
    38521 tcagggccgt gtccatgtga ccgattacac caacgcctct cgtaccatgt tgttcaacat
    38581 ccataccctg gactgggacg acaaaatgct ggaagtgctg gatattccgc gcgagatgct
    38641 gccagaagtg cgtcgttctt ccgaagtata cggtcagact aacattggcg gcaaaggcgg
    38701 cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag gccgcgctgt ttggtcagtt
    38761 gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact ggctgcttta tgctgatgaa
    38821 cactggcgag aaagcggtga aatcagaaaa cggcctgctg accaccatcg cctgcggccc
    38881 gactggcgaa gtgaactatg cgttggaagg tgcggtgttt atggcaggcg catccattca
    38941 gtggctgcgc gatgagatga agttgattaa cgacgcctac gattccgaat atttcgccac
    39001 caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca tttaccgggc tgggtgcgcc
    39061 gtactgggac ccgtatgcgc gcggggcgat tttcggtctg actcgtgggg tgaacgctaa
    39121 ccacattata cgcgcgacgc tggagtctat tgcttatcag acgcgtgacg tgctggaagc
    39181 gatgcaggcc gactctggta tccgtctgca cgccctgcgc gtggatggtg gcgcagtagc
    39241 aaacaatttc ctgatgcagt tccagtccga tattctcggc acccgcgttg agcgcccgga
    39301 agtgcgcgaa gtcaccgcat tgggtgcggc ctatctcgca ggcctggcgg ttggcttctg
    39361 gcagaacctc gacgagctgc aagagaaagc ggtgattgag cgcgagttcc gtccaggcat
    39421 cgaaaccact gagcgtaatt accgttacgc aggctggaaa aaagcggtca aacgcgcgat
    39481 ggcgtgggaa gaacacgacg agtaatgtca ggcacctgaa tcgattgcct gatgctgatc
    39541 gcctgatgcg acgcttacgc gtcttatcag gcctacgagc caatgccatt ttgtgtaggc
    39601 gggataaggc gttcacgccg catccggcat cacgcagact ctgaaaccgt gcgggggcaa
    39661 ccccgcacac atcaataatc ccacccttcc cctgtgctac acttcgcgcc attccttact
    39721 gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatctg
    39781 cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg
    39841 cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg
    39901 gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc
    39961 gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg
    40021 gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgcttc ctcaatgcgc
    40081 cggatatgta tatggagaag ctgattgtcg ggccgggagc caaaggcacc attgatctga
    40141 acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg
    40201 aactgacggt aacgattctg gctaaaccac gccacgatgc cgttatcgct gaaatgcagc
    40261 aactcggcgt acgcgtattt gctattccgg acggcgacgt tgcggcctca attctcacct
    40321 gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtgg
    40381 tttctgcggc ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc
    40441 atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct
    40501 gcaaagcgat gggcatcgaa gccggtaaag tattgcgtct gggcgatatg gcgcgcagcg
    40561 ataacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa ggtattagcc
    40621 gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgcaccattc
    40681 gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacattc
    40741 tctgagtgat ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg
    40801 agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag
    40861 gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc
    40921 ccgtgcttcc gtttaccgcc gggcaattta ccaagcttgg ccttgaaatc gacggcgaac
    40981 gcgtccagcg cgcctactcc tatgttaact cgcccgataa tcccgatctg gagttttacc
    41041 tggtcaccgt ccccgatggc aaattaagcc cacgattggc ggcactgaaa ccgggcgatg
    41101 aagtgcaggt ggttagcgaa gcagctggct tctttgttct ggatgaagta ccggactgcg
    41161 aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac
    41221 taggcaaaga tttagatcgc ttcaaaaatc tggtcctggt tcacgccgca cgttatgccg
    41281 ccgacttaag ctatttgcca ctgatgcaag aactggaaaa acgctacgaa ggcaaactgc
    41341 gcattcagac ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga cggataccgg
    41401 cattaattga aagtggggaa ctggaaagcg cgattggcct gccgatgaat aaagaaacca
    41461 gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaaag
    41521 agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt
    41581 actggtaagc ggttacttat cgataaacgg cacgatgagc aaatccgcac tcatcttatt
    41641 gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtgatgat gaccacagac
    41701 aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg
    41761 ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt
    41821 atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg
    41881 atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct
    41941 ggcgagctcc agggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac
    42001 gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa
    42061 cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaagtgaa ggccgcatca
    42121 agctcaactt gcccctccag atccttaatc ttataggcca cgctggcccc ttcatctgca
    42181 gaggatgcag cggcggcaag ctctttcagt ttggtcataa aagactctgc ttcttcgcgg
    42241 gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata
    42301 tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt
    42361 atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac
    42421 aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa
    42481 ggattttccg ggtattgtca tttgcatcgt ggaaataaca atcccggaaa aaaatagcag
    42541 taagagatat attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg
    42601 agtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag
    42661 catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc agacacccgg
    42721 taaaatcgct cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc
    42781 gccggaacgc ccgcgatcat gcaagcgctt aacagttact gccgctgttg gccagagcaa
    42841 gcacacaagg caaaacgccg cggtctgaat atcgagtaga ttcttacccg ccagtgagaa
    42901 aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat
    42961 acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc
    43021 ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc
    43081 ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc
    43141 attaaccgtt acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta
    43201 tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa
    43261 cagccagaat cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa
    43321 agccaatctc actcgtgctg caagtaaaat taacgagaac ttgtatgctt ctacagcgct
    43381 ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc
    43441 agagcaaaaa gcagcgaaag cgaaagctca ggaatacatg gcagcagtga tccgcacact
    43501 caccccatta atgaccaaaa tacaaagcca gaaaaaactg cagtcgctac taacggcggg
    43561 gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa
    43621 cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct
    43681 tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt
    43741 tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga
    43801 attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg
    43861 gttcacgagc tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt
    43921 gcaatcgcac caccggaaat gtacatcgat atggcgaagc gcgaagctga aggcagccac
    43981 atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaact
    44041 tctgccgcta tgctgaaaga catcggcgct cagtacatca tcatcggcca ctctgaacgt
    44101 cgtacttacc acaaagagtc tgacgaactg attgcgaaaa aattcgcggt gctgaaagag
    44161 cagggtctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggaaaa
    44221 actgaagaag tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca
    44281 ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa tcggtactgg caaatctgca
    44341 actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac
    44401 gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct
    44461 gcagaactgt ttgctcagcc agacatcgac ggcgcgctgg ttggcggtgc ttctctgaaa
    44521 gctgacgctt tcgcagtgat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga
    44581 caggtgccgg atttcatatc cggcacttac tttccttaac tcctcgcctt aacgcaaaat
    44641 ctcacactca tgatcctgga tttcctcggc tgaagcacgg ttcagcgtca gtagatttcg
    44701 ttgtgtcgcc agcaacacaa acgaattatc actctgccgt accatcgcca gcccgtagct
    44761 tcccatatgt tcccgcgcct caggcacttc ttctgcaagc atcataaatg ggctgcgttg
    44821 taccagttcg ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg
    44881 cagtggcaac cagcggctgc tgatgttcgc cagattttta tccagttgtt cgcgcacatc
    44941 aggacgaata caagagatat gaatatgaaa atggttttgc gtatgcccgg tgcgggagtt
    45001 gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc
    45061 acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact
    45121 ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtggac cattaagatc
    45181 ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt
    45241 ttgctgctga ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc
    45301 accggttaat ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa
    45361 aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta
    45421 acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat
    45481 cctagggaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt
    45541 tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa
    45601 acgcattttc gtactttttc gctacttcag cgtcgcgcgg acggtagtag tttttcgcgg
    45661 caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg
    45721 tgcctttttt ctcgaccact ttatcgacca ccgacaccgt tggctctgcg aggatagact
    45781 cactcggcgt gacgatttcg aatttatctt tccccagttc attcgctgcc agcagagctt
    45841 cgttttccca ggcgatcagt acatcgccaa tcccgcgctc gacaaaggtg ttagtggagc
    45901 cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg
    45961 cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggcagcc aggtagttcc
    46021 agcgtgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca
    46081 gatcgttcca gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg
    46141 aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc
    46201 gttccgcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac
    46261 cgttgattac cgacgtcgct tgtttacctg aaccaccgtg tgactgacga atcaccacgt
    46321 tatcaccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt
    46381 cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg
    46441 ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg
    46501 tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt
    46561 ggctataact tgcgggtata tgttgaggga ttaaaaaggc ggaagaaatc cgcctcatat
    46621 tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca
    46681 aaagtttgca aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca
    46741 ggcatttttg cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat
    46801 taatacagtt ttttcgcgca gtccagccag tcacctttga acggacgctt catgttttcg
    46861 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac acaacgaccg
    46921 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
    46981 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
    47041 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
    47101 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
    47161 tcttcacggc tgaattcaac ttccggaact acaacgaatt cacagccacc ggcaatggcc
    47221 gcagccaggg tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg
    47281 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
    47341 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg
    47401 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
    47461 ataaccacca gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg
    47521 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccgcc acggttgatc
    47581 atgtcagaca cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
    47641 ccgtcataaa tacccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
    47701 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacacctat tttcttaatc
    47761 atgactacct ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac
    47821 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
    47881 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
    47941 gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg
    48001 ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
    48061 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
    48121 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
    48181 acgatatcaa taatttcttg ccgttcttca tcaggcaatg cgcgatccag taatgactgt
    48241 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
    48301 aacagagcat cggcgcgatg ccagccgtac caagacaacc ccagcgccag cagaattgcg
    48361 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
    48421 gtccggcgca ccacccaccg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
    48481 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatac cagatgttga
    48541 atacccgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
    48601 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
    48661 tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
    48721 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
    48781 aacgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
    48841 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
    48901 cattaagcag caggcaaatt gaggataaaa aaaaccccac atcatgtggg ggaagacagg
    48961 gatggtgtct atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc
    49021 aatagcttca acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt
    49081 tgttgatgtt tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg
    49141 ttgcggactt ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc
    49201 tgtgcgcgca cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc
    49261 agttcgctaa cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc
    49321 tgctgacgct gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc
    49381 tgcgtaagtt cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt
    49441 aatgaactga ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc
    49501 tcccaaaatc tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat
    49561 gcgtcagggg gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt
    49621 ctgcctcgga ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct
    49681 gacttcccta ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga
    49741 tggggaacag gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat
    49801 gatgccgaag aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc
    49861 tgtcattatg ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg
    49921 cgcagatgac tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc
    49981 aatcctgcgc cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac
    50041 actggaagtt gatgccttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca
    50101 aacgctggag ttaaccggta ctgagtttac cctgctctat ttgctggcac agcatctggg
    50161 tcaggtggtt tcccgtgaac atttaagcca ggaagtgttg ggcaaacgcc tgacgccttt
    50221 cgaccgcgct attgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga
    50281 tggtcacccg tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg
    50341 ataggcagct taaccgcgcg catcttcgcc atcttctggc tgacgctggt gctggtgttg
    50401 atgttggttt tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc
    50461 gaacagcgtc aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg
    50521 cccaacgatt taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca
    50581 ggacagcgtt tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa
    50641 atgcagatca ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa
    50701 aagtatggcc gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac
    50761 caactttatc tgattcgtcc ggccagtagt tctcaatccg atttcattaa cttactgttt
    50821 gaccgcccgc tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg
    50881 ttggcctgga gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc
    50941 cagggaaact tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca
    51001 ggtgccagtt ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc tcagcagcgt
    51061 ctgctttctg atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg
    51121 gcgttactgc gccgtcgtag cggtgaaagc aaggaactcg agcgtattga aaccgaagcg
    51181 caacgtctgg acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac
    51241 gcgctggtta gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg
    51301 gcgttcgaag ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc tgggccgtgg
    51361 ccgctgtacg gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct
    51421 ctgcgttatt cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc
    51481 attacggtgg acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt
    51541 ccgttctatc ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg
    51601 gcgattgttg aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg
    51661 ctgggcggtt tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat
    51721 ttgtaggcag gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga
    51781 cgctgatagc gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga
    51841 aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga
    51901 taacccggcg gcggagagta aacggtgata ctgatcgtca tcaaacggca tatgccatgc
    51961 gatggcggca gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc
    52021 gtccgcagac actttccctg gcgctatcac gctatacaac caccccacct tacctgtgtt
    52081 ttgcatcaac tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga
    52141 gcgcggctgg ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatatgaac
    52201 attactttcc gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa
    52261 ctccgcctgt tccggaaatt cccgcaccca gtagagatag tgctcacgag gataatgaca
    52321 c
//
LOCUS       DAJVPT010000014        49522 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVPT010000014 DAJVPT010000000
VERSION     DAJVPT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1171
                     /locus_tag="OQM47_000821"
     CDS             <1..1171
                     /locus_tag="OQM47_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1479084.1"
                     /translation="SVCLSTWKAALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKN
                     LTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSY
                     FAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAI
                     NGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTG
                     DVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHYNSTHVV
                     GTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGV
                     TMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1168..2133
                     /gene="yggF"
                     /locus_tag="OQM47_000822"
     CDS             1168..2133
                     /gene="yggF"
                     /locus_tag="OQM47_000822"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1479085.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2155..2664
                     /gene="fumE"
                     /locus_tag="OQM47_000823"
     CDS             2155..2664
                     /gene="fumE"
                     /locus_tag="OQM47_000823"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1479086.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2661..3374
                     /locus_tag="OQM47_000824"
     CDS             2661..3374
                     /locus_tag="OQM47_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1479087.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3346..4023)
                     /locus_tag="OQM47_000825"
     CDS             complement(3346..4023)
                     /locus_tag="OQM47_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1479088.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4017..4694)
                     /locus_tag="OQM47_000826"
     CDS             complement(4017..4694)
                     /locus_tag="OQM47_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1479089.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4682..5065)
                     /locus_tag="OQM47_000827"
     CDS             complement(4682..5065)
                     /locus_tag="OQM47_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1479090.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5081..5389)
                     /locus_tag="OQM47_000828"
     CDS             complement(5081..5389)
                     /locus_tag="OQM47_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479091.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5390..5968)
                     /locus_tag="OQM47_000829"
     CDS             complement(5390..5968)
                     /locus_tag="OQM47_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1479092.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5991..6422)
                     /locus_tag="OQM47_000830"
                     /pseudo
     CDS             complement(5991..6422)
                     /locus_tag="OQM47_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6794..7813
                     /gene="epd"
                     /locus_tag="OQM47_000831"
     CDS             6794..7813
                     /gene="epd"
                     /locus_tag="OQM47_000831"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1479093.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7863..9026
                     /gene="pgk"
                     /locus_tag="OQM47_000832"
     CDS             7863..9026
                     /gene="pgk"
                     /locus_tag="OQM47_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1479094.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9241..10320
                     /gene="fbaA"
                     /locus_tag="OQM47_000833"
     CDS             9241..10320
                     /gene="fbaA"
                     /locus_tag="OQM47_000833"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1479095.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10511..11371
                     /gene="mscS"
                     /locus_tag="OQM47_000834"
     CDS             10511..11371
                     /gene="mscS"
                     /locus_tag="OQM47_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1479096.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11510..12145
                     /gene="argO"
                     /locus_tag="OQM47_000835"
     CDS             11510..12145
                     /gene="argO"
                     /locus_tag="OQM47_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1479097.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12238..12978
                     /locus_tag="OQM47_000836"
     CDS             12238..12978
                     /locus_tag="OQM47_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1479098.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13145..14095
                     /locus_tag="OQM47_000837"
     CDS             13145..14095
                     /locus_tag="OQM47_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1479099.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14037..15515)
                     /gene="scpC"
                     /locus_tag="OQM47_000838"
                     /pseudo
     CDS             complement(14037..15515)
                     /gene="scpC"
                     /locus_tag="OQM47_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15539..16324)
                     /gene="scpB"
                     /locus_tag="OQM47_000839"
     CDS             complement(15539..16324)
                     /gene="scpB"
                     /locus_tag="OQM47_000839"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1479100.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16335..17330)
                     /gene="meaB"
                     /locus_tag="OQM47_000840"
     CDS             complement(16335..17330)
                     /gene="meaB"
                     /locus_tag="OQM47_000840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1479101.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17323..19467)
                     /gene="scpA"
                     /locus_tag="OQM47_000841"
     CDS             complement(17323..19467)
                     /gene="scpA"
                     /locus_tag="OQM47_000841"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1479102.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19671..20564)
                     /gene="argP"
                     /locus_tag="OQM47_000842"
     CDS             complement(19671..20564)
                     /gene="argP"
                     /locus_tag="OQM47_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1479103.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20947..21606
                     /gene="rpiA"
                     /locus_tag="OQM47_000843"
     CDS             20947..21606
                     /gene="rpiA"
                     /locus_tag="OQM47_000843"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1479104.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21862..23094
                     /gene="serA"
                     /locus_tag="OQM47_000844"
     CDS             21862..23094
                     /gene="serA"
                     /locus_tag="OQM47_000844"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1479105.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23323..23382
                     /gene="ibsC"
                     /locus_tag="OQM47_000845"
     CDS             23323..23382
                     /gene="ibsC"
                     /locus_tag="OQM47_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1479106.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23715..23774
                     /locus_tag="OQM47_000846"
     CDS             23715..23774
                     /locus_tag="OQM47_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1479107.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23875..24477)
                     /locus_tag="OQM47_000847"
     CDS             complement(23875..24477)
                     /locus_tag="OQM47_000847"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1479108.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24498..24681)
                     /gene="ssrS"
                     /locus_tag="OQM47_000848"
     ncRNA           complement(24498..24681)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQM47_000848"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24723..25052)
                     /gene="zapA"
                     /locus_tag="OQM47_000849"
     CDS             complement(24723..25052)
                     /gene="zapA"
                     /locus_tag="OQM47_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1479109.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25220..25798
                     /gene="ygfB"
                     /locus_tag="OQM47_000850"
     CDS             25220..25798
                     /gene="ygfB"
                     /locus_tag="OQM47_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1479110.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25824..27149
                     /gene="pepP"
                     /locus_tag="OQM47_000851"
     CDS             25824..27149
                     /gene="pepP"
                     /locus_tag="OQM47_000851"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1479111.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27146..28324
                     /gene="ubiH"
                     /locus_tag="OQM47_000852"
                     /gene_synonym="visB"
     CDS             27146..28324
                     /gene="ubiH"
                     /locus_tag="OQM47_000852"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1479112.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28347..29549
                     /gene="ubiI"
                     /locus_tag="OQM47_000853"
                     /gene_synonym="visC"
     CDS             28347..29549
                     /gene="ubiI"
                     /locus_tag="OQM47_000853"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1479113.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29623..29706)
                     /locus_tag="OQM47_000854"
     CDS             complement(29623..29706)
                     /locus_tag="OQM47_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479114.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29997..31091
                     /gene="gcvT"
                     /locus_tag="OQM47_000855"
     CDS             29997..31091
                     /gene="gcvT"
                     /locus_tag="OQM47_000855"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1479115.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31115..31504
                     /gene="gcvH"
                     /locus_tag="OQM47_000856"
     CDS             31115..31504
                     /gene="gcvH"
                     /locus_tag="OQM47_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1479116.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31623..34496
                     /gene="gcvP"
                     /locus_tag="OQM47_000857"
     CDS             31623..34496
                     /gene="gcvP"
                     /locus_tag="OQM47_000857"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1479117.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34763..35506
                     /locus_tag="OQM47_000858"
     CDS             34763..35506
                     /locus_tag="OQM47_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1479118.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35563..36996)
                     /gene="bglA"
                     /locus_tag="OQM47_000859"
     CDS             complement(35563..36996)
                     /gene="bglA"
                     /locus_tag="OQM47_000859"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1479119.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37041..37351
                     /locus_tag="OQM47_000860"
                     /pseudo
     CDS             37041..37351
                     /locus_tag="OQM47_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37515..38174
                     /locus_tag="OQM47_000861"
     CDS             37515..38174
                     /locus_tag="OQM47_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1479120.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38370..39350)
                     /gene="ygfZ"
                     /locus_tag="OQM47_000862"
     CDS             complement(38370..39350)
                     /gene="ygfZ"
                     /locus_tag="OQM47_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1479121.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39593..39859
                     /gene="sdhE"
                     /locus_tag="OQM47_000863"
     CDS             39593..39859
                     /gene="sdhE"
                     /locus_tag="OQM47_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1479122.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39840..40247
                     /locus_tag="OQM47_000864"
     CDS             39840..40247
                     /locus_tag="OQM47_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1479123.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40287..40808)
                     /gene="fldB"
                     /locus_tag="OQM47_000865"
     CDS             complement(40287..40808)
                     /gene="fldB"
                     /locus_tag="OQM47_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1479124.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40920..41816
                     /gene="xerD"
                     /locus_tag="OQM47_000866"
     CDS             40920..41816
                     /gene="xerD"
                     /locus_tag="OQM47_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1479125.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41841..42551
                     /gene="dsbC"
                     /locus_tag="OQM47_000867"
     CDS             41841..42551
                     /gene="dsbC"
                     /locus_tag="OQM47_000867"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1479126.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42557..44290
                     /gene="recJ"
                     /locus_tag="OQM47_000868"
     CDS             42557..44290
                     /gene="recJ"
                     /locus_tag="OQM47_000868"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1479127.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44381..45479
                     /gene="prfB"
                     /locus_tag="OQM47_000869"
     CDS             join(44381..44455,44457..45479)
                     /gene="prfB"
                     /locus_tag="OQM47_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1479128.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45489..47006
                     /gene="lysS"
                     /locus_tag="OQM47_000870"
     CDS             45489..47006
                     /gene="lysS"
                     /locus_tag="OQM47_000870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1479129.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47049..47597)
                     /gene="idi"
                     /locus_tag="OQM47_000871"
     CDS             complement(47049..47597)
                     /gene="idi"
                     /locus_tag="OQM47_000871"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1479130.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47652..47723
                     /locus_tag="OQM47_000872"
     CDS             47652..47723
                     /locus_tag="OQM47_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479131.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47720..47845)
                     /locus_tag="OQM47_000873"
     CDS             complement(47720..47845)
                     /locus_tag="OQM47_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479132.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47847..49295)
                     /gene="uacT"
                     /locus_tag="OQM47_000874"
     CDS             complement(47847..49295)
                     /gene="uacT"
                     /locus_tag="OQM47_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1479133.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11954 a        12402 c        13341 g        11825 t
ORIGIN      
        1 cagcgtctgt ttatcgacct ggaaagcggc gttactcggt agtgaacata aacgcgtacc
       61 cgacgattta gaaaatcatc cggtcattac cgggcatgaa tgtgccgggg ttattgtcga
      121 agtgggtaaa aatctcactg gcaaatataa aaaaggccag cgttttgtat tgcaaccggc
      181 gatggggtta ccaagcggat attcagcggg ctacagctac gaatattttg gcggcaatgc
      241 cacttatatg attattcccg aaatcgccat taatttgggc tgcgtattac cgtatcacgg
      301 ctcttatttt gctgcggcgt cgctggcaga gcctatgtgc tgcattattg gtgcttatca
      361 tgccaattat cacaccacgc aatatgttta tgagcatcgc atgggcgtca aacctggcgg
      421 caatattgca ctgctggcgt gtgcgggtcc gatgggcatt ggcgctatcg attacgccat
      481 taacggtggc atacaaccgt cacgggtggt ggtggtcgat atcgacgaca aacgcctggc
      541 gcaggtgcag aagctgctgc cggtggatct ggcggccagt aaaggcattg agctggtgta
      601 tgtgaatacc aaagggatga gcgatcctat ccagatgctg cgggcgctga caggagatgt
      661 cgggttcgat gacatttttg tttatgcggc ggtgcctgct gtcgttgaga tggctgacga
      721 attactggcg gaagatggct gtctgaactt ctttgccggg ctgacggata aaaacttcaa
      781 agtgccgttt aatttctaca acgtccatta caacagcacg cacgtagtcg gtacatccgg
      841 cggttcaacg gacgacatga aagaggcgat tgcccttagc gccacaggac agttacagcc
      901 gtcgtttatg gtgacccata ttggtggcct ggatgcggtg ccagaaaccg tgctcaatct
      961 gccggatatc cctggcggta aaaaactcat ttataacggc gtgaccatgc cgctcactgc
     1021 cattgccgat tttgccgaaa aaggcaaaac cgatccgctg tttaaagagt tggcgcggct
     1081 ggttgaggaa acgcacggca tctggaatga acaggccgag aaatatctgc tggcacaatt
     1141 tggcgttgat atcggggagg ccgcgcaatg atgtccctgg cgtggccatt atttcgcgtt
     1201 acggaacagg cagcgttggc tgcctggccg caaaccggat gtggcgacaa aaataaaatt
     1261 gatggcctgg cggtcaccgc gatgcgccag gcattaaacg acgtcgcttt tcgtggacga
     1321 gtggttatcg gcgaaggaga aattgaccat gcgccgatgc tgtggattgg cgaagaggtg
     1381 ggcaaaggcg atgggccaga agtcgatatc gcggttgatc ccattgaagg cacgcggatg
     1441 gtggcgatgg ggcagagcaa tgcgctggcg gtcatggctt tcgctcctcg agatagcctg
     1501 ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta atcgactggc tgccggggcg
     1561 attgatctgt ctttgcctct ggcggacaac ttgcgtaacg tggcgcgggc gttaggtaaa
     1621 ccgctggaca agctgcgcat ggttacgctg gataaaccgc gtctgagtgc cgcgattgaa
     1681 gaagcaacac agctgggcgt gaaggttttt gccctgccgg atggcgatgt cgccgccagc
     1741 gtgctgacct gctggcagga taatccttac gatgtgatgt acaccatcgg cggtgcgcca
     1801 gaaggcgtga tttctgcctg tgccgttaaa gcgttaggcg gggatatgca ggccgagctg
     1861 attgactttt gtcaggctaa aggggattac acggaaaatc ggcagattgc agagcaggag
     1921 cgcaagcgtt gtaaggcaat gggcgttgac gttaaccgtg tgtactcgct cgatgaactg
     1981 gtgaggggga acgatatcct ctttagcgct acgggcgtga cgggcggcga gttggtgaac
     2041 ggtatccaac agacggcgaa tggggtgcgg acgcagacat tactgatcgg cggcgcggac
     2101 caaacgtgta atataataga ctccctgcat tgatggtgat ctatcggaag accaatggcg
     2161 acgctgacag aagatgatgt gcttgagcaa ctggatgcac aggacaattt attttcattt
     2221 atgaaaactg cgcattctat tttgctccag gggatacgcc agtttctgcc gtcgctgttt
     2281 gtcgataacg atgaagagat cgtcgaatat gcagtgaagc cgttactcgc ccaaagcggc
     2341 ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg cgctggggaa aatggataaa
     2401 tggctgtatg ccgatatcac gcacttttcc cagtactggc attacctgaa cgaacaggat
     2461 gaaacgcccg gatttgccga tgatataacc tgggatttta tcagcaatgt caacagtata
     2521 actcgcaatg cgacgctcta cgatgcgtta aaagcgatga agttcgccga tttcgccgtc
     2581 tggtcagagg cgcgttttag cggaatggtc aaaacggcgc tgacgctggc agtaacgacc
     2641 accttaaagg aattaacgcc gtgaaaattg aattaacggt gaatgggctg aacgtgcaag
     2701 cgcagtacca tgatgaagag atagaacgcg tgcataaact gttgctgcgt atgctggcgg
     2761 ccttacagac ggtaaatccg cagcggcgta cggtggtttt tctttgcgct ccgccgggga
     2821 cgggtaaatc taccctaacc accttctggg aatatctcgc gcaacaagac ccggagctgc
     2881 ccgctattca ggcgctcccg atggatggtt ttcaccatta caatagctgg ctggacgcgc
     2941 atcaattgcg ccccttcaaa ggcgcaccag agacatttga cgttgcgaaa ctggcggaaa
     3001 atctgcgcca ggtggtggaa ggggattgta cgtggccgca gtacgatcga caaaagcatg
     3061 atcctgttga agatgcgttg cacgttaccg caccactcgt catcgtcgaa ggaaactggt
     3121 tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt ttgtgatttc tcgatcttta
     3181 tcaacgcgcc agctgctgcg ttgcgggagc gtctggtggg gcgcaagctg gcgggagggg
     3241 tatcgctggc agatgcagaa gccttttatg accgtaccga cgggccgaat gttcgccggg
     3301 ggctggaaga gagtctgcct gcaaatttga ccttaatgat gaccgctacg ggtgaatatc
     3361 gtctggtgga ttaacgttcc tgattacacc atcttccagc cgcaccacac gagagaaatg
     3421 acgccgggta aatgcggcgt catgactgat tgctaccacg ctcgtgcctc gctggcggca
     3481 cttctccagc cagctttcaa acacgctcaa ccaattttcg tcaaaatccc gactcggctc
     3541 gtccagcagt aatagtggcg gagagagtgc ttccaggcag gcgacggcga ccatccgtcg
     3601 ctgcgcatta tgcagatcca acgggtgcga gttggcagtg tctgctaact gacaacattg
     3661 cagggcagca tgggtacgtt gagtgatttc atccgccggg catttttgca gtttcaaacc
     3721 aaatgcgatc tcgtcagcga cggtgctgtg aaaaagttgg ttttccgctt cctgaaacag
     3781 tacgccaacc tttgccgcgc gctggcggtt tttcaggttt ttcatcgcct gttgttgcag
     3841 cataaccgta ccggctgtgg gcgtaagaag acctgccatt actcgtaaca gcgtcgattt
     3901 ccctgcgcca ttgtcaccgg tcagcgccaa ccattcacct tgtttgagtt gcagagaaat
     3961 gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa gatatcttat ttaacgttaa
     4021 catagtggct ggagcgctcc gttttgcaat tgccacgctt gctggcagta gttcaggaaa
     4081 ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac gttcaaacgc ccagtgttgt
     4141 aagcgctgca acagcatttc gctggcctgc ggtgtcaggc ggctaaaggc ttcatcaaga
     4201 attaagagct ttggctgcat cgcgatggcg caggcgatga tcacgcgctg tgtctctcca
     4261 ccggagagtg ttgccgggtg acgatggcgc aacggctggc actcggtcag agtcagggcg
     4321 gcatcaatcc gcgccataat ctctgcttca gcaagacaca gattttctgg cccaaaggca
     4381 acttcttctt ccacgctaaa ggtacagccg gagagctgca aatagggcga ttgctggacg
     4441 agctggatgg tggcggattg ttcattaagc gggagatgcc caatgggcgt accgaggagc
     4501 gtccccgtgc ctgttatctc tcttggcaga aagtccggat accagcccgc catcagctgt
     4561 gccagcgtac ttttgccact gccgttgtca ccaaagatgg cgaccatccc cggtttgaca
     4621 tagtgaaagt cataacagaa cggaggatgc gttgagtgtg tcgggcaata acggaactgt
     4681 tctaacgtaa ccatatccac gctccgaatt ctgtcagcat cagcaagatc atggtgtaac
     4741 gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga
     4801 tccgaaatgc ccgcatatcc agcgccgcac cacggacagc cagatcgtta agcgcattgt
     4861 gtgttagcgg aataattaac gcgggcatag cacgtaaacg ctgataccag ccctcatcca
     4921 gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc taattgccgt tttaactgct
     4981 caacgaccaa taacggcccg gcaaacagat aagcgacgcc tggcggcagg cgagaagcaa
     5041 acagagcgcg aataaaacat tgcacgggga cgtactgcat tcacaactgt gaggttgaaa
     5101 caatcgccag aatcagcaac cagagcgtaa tagcatgcga ccaccgttct ggcgagcgtg
     5161 gcgtgccgct tagccattct ggtagccagc caccgtgtac cagccacagc ccggctccca
     5221 gcgagaacat cagccagacg acatattttg cccgccgccg cgtggccttt agcgcaataa
     5281 ggcagaaaaa ggtcgccgcg ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg
     5341 tggtgcaggc cgctaatgcc cacagtgtta atgaggtaaa tggatgcatt tagcgcacgg
     5401 cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga
     5461 caatcaccgc tgtaagaatt ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg
     5521 actccaccag gttttgcccc atcgagtgca tccaggcaac gaacaaatcg gctccgctac
     5581 cggtgacacc gccgaacagc gcagtacgca gcggtacagc gacgaccgtt accgcaagcg
     5641 taataatcac gccgctgaca acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca
     5701 gccaaccgga gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg gcgatcgggt
     5761 cggtcagcaa tccccagagt aaattggtca gtaagccggt cagcattccg atcaccggac
     5821 caagcagtac cgcgctaata agtgtgccga tggagtcgag aaatataggt aatttgacca
     5881 tgctggccag ttgcccgccg atcatattga tggcgataga tataacgatc aacaccagtg
     5941 cctgactgga aaagtggcta cgcgccataa gaaaacctct ttgaaagtag ttatttaacg
     6001 ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct aaacgccagt
     6061 tcctcaaggc taccgatcac cagttcaagc gtggtccgca cggtacagcc gggcatcata
     6121 tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc
     6181 tgttccagcg tgccgtttag tgagatcact tcgaatgtac cgttcaggag cgtggtgcct
     6241 tcttgcccgg catagcgcaa ggcgacgtca gtcaggctac cggtacagcc tgcaatctag
     6301 gcggcatgaa gttgatgttg ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg
     6361 gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat atgagtaggg caatgtcatc
     6421 attggagtac tccgaaactc ggacgtattg cggataatat cgacgctgca tcagtcatat
     6481 tctgatataa gtcagggttt gtgacggcac aacgaaaaga ttttgctacg cttattcgtg
     6541 attctgcaaa gtggaataaa ttccacaggg gcggggctga aaacgtgacg taagtcagat
     6601 aattgctttc tttgactgga caatcattcc ttttattcca cgtttcgctt atcctagctg
     6661 aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt
     6721 aaggtttccc cggacgattt gctggattac tctgtctggc aaatttgctc agggaaaacc
     6781 ttgcaggaga tctatgaccg tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa
     6841 tgtggttcgt gctttgtatg aatccggacg ccgggcggaa attaccgtgg tggcaatcaa
     6901 cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa tatgacacca gccatggccg
     6961 ttttgcatgg gaagtacgac aggaacgcga tcaacttttt gttggtgatg acgccatccg
     7021 cgtattgcat gaacgttcac tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt
     7081 cctcgactgc accggcgtat atggctcccg cgagcatggc gaagcgcata ttgccgccgg
     7141 ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat ctcgacgcga ccgttgttta
     7201 cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc gtttctaacg cttcctgtac
     7261 cacgaattgc ataattcccg tcatcaaatt gttagatgat gcgtacggta ttgagtccgg
     7321 cactgtgacc acaattcact ccgccatgca cgatcaacag gttattgatg cataccatcc
     7381 tgacctgcgt cgcacccggg cagccagcca gtcgatcatt ccggtcgata ctaaactggc
     7441 cgctggtatc acacgatttt ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg
     7501 tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa
     7561 agccaatgaa gtcaacctgt tgctgcaaaa agcagcacaa ggtgcatttc atggtatagt
     7621 tgactatacg gaattgccgt tggtctctgt agattttaac cacgatccgc acagtgccat
     7681 tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg
     7741 cgataacgaa tggggctttg ctaaccgaat gctcgacacg acgttagcta tggctactgt
     7801 tgctttcagg taagacgcaa gcagcgtctg caaaactttt agaatcaacg agaggattca
     7861 ccatgtctgt aattaagatg accgatctgg atcttgctgg gaaacgtgta tttatccgtg
     7921 cggatctgaa cgtaccagta aaagacggga aagtaaccag cgacgcgcgt atccgtgctt
     7981 ctctgccgac catcgaactg gccctgaaac aaggcgcaaa agtgatggta acttcccacc
     8041 tgggtcgtcc taccgaaggc gagtacaacg aagaattctc tctgctgccg gttgttaact
     8101 acctgaaaga caaactgtct aacccggttc gtctggttaa agattacctc gacggcgttg
     8161 acgttgctga aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga
     8221 aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg
     8281 cattcggtac tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg
     8341 ttgcgtgcgc aggcccgctg ctggcagctg aactggacgc gctgggtaaa gcactgaaag
     8401 aacctgctcg cccgatggtg gctatcgttg gtggttctaa agtatctacc aaactgaccg
     8461 ttctggactc cctgtctaaa atcgctgacc agctgattgt tggtggtggt atcgctaaca
     8521 cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct gacctggttg
     8581 acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat gttcgcgtag
     8641 caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg
     8701 acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa atcctgaaga
     8761 atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg aacttccgca
     8821 aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc atcgctggcg
     8881 gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc tcctacatct
     8941 ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct gcagtagcga
     9001 tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg cacttgtttc
     9061 agcgcgcttt tagagcacgc attttttcat actctaaata attcgagttg caggaaggcg
     9121 acaagagagt gaatccccag gagcttacat aagtaagtga ctggggtgag cgaacgcaga
     9181 cgcagcacat gcaacttgaa gtattatgag tataaggccc gacgatacag gacaagagac
     9241 atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa
     9301 gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg cgtcggtact
     9361 gactccatca acgccgtact ggaaactgct gctaaagtta aagcgccggt tatcgttcag
     9421 ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag
     9481 ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat ggctgaacat
     9541 tatggtgttc cggttatcct gcacactgac cactgcgcga agaaactgct gccgtggatc
     9601 gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta ccggtaagcc gctgttctct
     9661 tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa
     9721 tacctggagc gtatgtccaa aatcggcatg actctggaaa tcgaactggg ttgcaccggt
     9781 ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta cacccagccg
     9841 gaagacgttg attacgcata caccgagctg agcaaaatca gcccgcgttt caccatcgct
     9901 gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgtggttct gactccgacc
     9961 atcctgcgtg attctcagga atatgtttct aagaaacaca acctgccgca caacagcctg
    10021 aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga ctccgtaagc
    10081 tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg ggaaggcgtt
    10141 ctgaactact acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc
    10201 gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc cggtcagact
    10261 tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga cgttctgtaa
    10321 gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa
    10381 ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
    10441 tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
    10501 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
    10561 ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
    10621 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
    10681 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat
    10741 tatcgccttt acgctaatcg ctgccctggg acgcgtgggt gtacaaacag cgtcagtcat
    10801 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactttctaa
    10861 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga
    10921 cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    10981 tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa
    11041 cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga
    11101 tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa
    11161 agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt
    11221 ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    11281 taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt
    11341 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    11401 gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc gctaataaca
    11461 atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta
    11521 ttactttcaa ggtcttgctc ttggggcagc tatgattctg ccgctcggcc cacaaaatgc
    11581 ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgcgc
    11641 tatcagcgat ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt tattgatgca
    11701 gtcgccgtgg ttgctagcgc tggtcacctg gggcggcgta gtcttcttgc tctggtatgg
    11761 ttttggcgct tttaaaacag cgatgagtag taatattgag ttagctagtg ccgaagtcct
    11821 gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    11881 tgtttacctg gatacttttg ttgtactggg cagccttggc gggcagcttg acgtcgaacc
    11941 aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    12001 tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa
    12061 tctggtagtg ggatgtgtca tgtggtttat agccttgcag ctggcgagag acggtattgc
    12121 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    12181 agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    12241 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    12301 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagtgt ggatgcggtg
    12361 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctacagcc
    12421 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc
    12481 gcgaaaaaag atatcagctc agcgaattta cgcacccagc cagattatga ctatcaggat
    12541 ggtaaaagta tcctgaaagg ctaccgagct gtgagaacgg tggaagtcac gctccgtcag
    12601 ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt
    12661 tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    12721 attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg
    12781 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    12841 atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc cgctattcag
    12901 tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa
    12961 acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc
    13021 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    13081 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcggggagg
    13141 ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa
    13201 taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat
    13261 tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag
    13321 cctcaataaa gccggtcagg aactgtatca aaaactgttc ccggtttatc aacgactttc
    13381 tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat
    13441 agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga
    13501 aggcgtgacc gcccagccag ttgagttcag cgaaaacggc gttatcgata atctttttga
    13561 ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga
    13621 aaacctgact atcagtgaat taccaccgct gcgattgggc tttctcgttt ctcgccgtta
    13681 tgaagagcgt caagagcaag agctgcttca ggagctgccc tggctacaaa tgcgctttca
    13741 aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc
    13801 caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca
    13861 ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata
    13921 ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca
    13981 tcgttatacc gccacgatct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    14041 cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    14101 tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc ccgatacata
    14161 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    14221 tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    14281 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg agccataaag
    14341 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    14401 accccggcta cgtgcgttga atttgcatgc ccgtaaatat caaactccag gccgacgttc
    14461 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    14521 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    14581 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    14641 accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg gttttccccc
    14701 agacgtgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    14761 ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc
    14821 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    14881 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    14941 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    15001 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    15061 agcagctagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc
    15121 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    15181 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    15241 cgtaaactgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    15301 acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    15361 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg cgggtagggc tttcggcgaa
    15421 cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    15481 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    15541 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct tcctggtaat
    15601 cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca tcggagttca
    15661 tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg gctaacggcg
    15721 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca
    15781 caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgattggc gaagcggtaa
    15841 aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca
    15901 ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga
    15961 tgatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca
    16021 tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc aatggatcat
    16081 cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga cctgcggaga
    16141 agactttgga tccacttggc gcccgcaaaa tgatacagcg aatttccggc cggttgagat
    16201 cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta agttttcgtc
    16261 catagttaaa ctcaatgacc gccactttgt tgatagtggc aacgttcaca tactgataag
    16321 acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg
    16381 ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga
    16441 tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg cttacgcagc
    16501 cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt
    16561 ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa
    16621 gtcagacccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg
    16681 tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg
    16741 agcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg
    16801 gcaatttgca acgagataaa acagtccacc atgcgggcga cttccgtttc cgactgcgcg
    16861 atgccaaccg tttcgacaat cactacgtca taacccgccg cttcgcataa cagcattaat
    16921 tcccgtgctc gctgactggc accgcccaga tgaccggagg agggtaccgg gcgaataaac
    16981 gccgcctcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca
    17041 gtgaccgggc tgctgggatc gaccgcaata acagcaacct ttaatccctc acgaatcaac
    17101 aacatgccaa aggcttcaag aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc
    17161 agtcgcaggg cgttaccaca gaacggcata atagcgtcaa gcagctgtgt acttagcgcc
    17221 tgatgacgtg ggtgacggct ttccaccagc gtcatggctt gggcgagcgt ggcacgctca
    17281 ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct
    17341 tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac cataaatcgc
    17401 cgccacgccg cgctcttgca ggaaggcgta atcctgcggc ggaatgacgc cacccgcgac
    17461 cacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt
    17521 tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcgtttt ctacggctag
    17581 gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc
    17641 ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc
    17701 gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat
    17761 ggcatcgaac tcggaggccg atttctcaga ctgatgatag ctttgcgcaa tcacgccggt
    17821 aacacactgg cttggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc
    17881 acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg
    17941 ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc
    18001 gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag
    18061 tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt
    18121 gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag
    18181 acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct
    18241 ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc
    18301 gacggtgtgg cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg
    18361 tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg
    18421 agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt tgttatacgg
    18481 atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt
    18541 cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    18601 catggcgacg ttcataaaca gatccatgcc gataccgaag aataacgaca ggcgaggagc
    18661 gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcggc
    18721 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcccccatgt ggtaaccgct
    18781 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat
    18841 aatgcgcatt gacggttttg gtgggtaaat ataggtgttg cggcagaggt actctttgag
    18901 aatatcgttt tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttctgcggc
    18961 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    19021 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    19081 gcccgctttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc cacggtgggt
    19141 ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa
    19201 agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc
    19261 ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt
    19321 cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc
    19381 ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag
    19441 ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg
    19501 tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca
    19561 gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa
    19621 aagaagtgag gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga
    19681 cgaaggactt tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt
    19741 tcaggagcaa agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca
    19801 atcagttcac cgctggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg
    19861 gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggtacg
    19921 ctgccaggag gaagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg
    19981 tcaaacgcga ccactggcgc tttcaataat gccgaacgcg ttacgccgtt aggaaaatat
    20041 ttttcggcaa acggttttga gctgacgaac agatagtcga gcgcaccgag tttatcgaca
    20101 agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc
    20161 agacgttcct gagtacgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca
    20221 gccaacacag gagccagtgc cggaagcaac cacgtcgcca gactgtcggc gttgaccgcc
    20281 agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc
    20341 aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt
    20401 acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag
    20461 acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc
    20521 accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc
    20581 tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc
    20641 cgtcggcggg taacatttgt atcaatggtt aaacgcgcag cttaaaagat cagggatttg
    20701 cgctattgca tttcgcttag cgacttcgcg ccctggttat gttgaatggc gtggcgttat
    20761 tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc
    20821 gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacc cggcttgcca
    20881 acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa
    20941 acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt
    21001 cagcccggta ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgcg
    21061 ctcggcacga tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa
    21121 aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc
    21181 tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg
    21241 gcgctgaccc gtgaaaaaat cattgcttcg gttgcggaaa aatttatctg tattgccgac
    21301 gcctccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg
    21361 gcacgtagtg cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga ataccgtcag
    21421 ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac
    21481 ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggcttgttt
    21541 gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg
    21601 aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac
    21661 atgtgtcacg cttttaccag gcaattgtcg attgctctaa ataaatcctc taaaccagca
    21721 tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat
    21781 attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt
    21841 tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt
    21901 ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc
    21961 aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat
    22021 gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca
    22081 gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg
    22141 gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg
    22201 gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa
    22261 gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag
    22321 ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg
    22381 ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag
    22441 gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag
    22501 aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg
    22561 ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg tgatgcgctg
    22621 gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat
    22681 agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt
    22741 ggcggttcga ctcaggaagc gcaggagaat atcggcttgg aagttgcggg taaattgatc
    22801 aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca
    22861 ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg
    22921 ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc
    22981 gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg
    23041 ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc
    23101 ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac
    23161 cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga
    23221 tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac
    23281 ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc
    23341 atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt ctcaagggag
    23401 gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca
    23461 gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg
    23521 tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat ggggtttttc
    23581 gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat ttaaagagag
    23641 cttacgatag ctgggcagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
    23701 gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta ctcataagtt
    23761 tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc cttactcact
    23821 aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc gcccttacca
    23881 ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg
    23941 gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt tatagtgctg
    24001 ccagttttgt aaggtccgat cataaaaacc accgcccatt cccagacgct gaccgtactc
    24061 atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg
    24121 cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcgc tttgcggatg
    24181 gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg
    24241 cttaccggcg cgccagagtt gttctattag cggctgggtg tcgagttcac catcaaaaga
    24301 gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca tccgggtagc
    24361 ggcttgttga cccatttcca gctgttgttc cggcgttaac gcacgacgac gttgccgaat
    24421 cattttgcgg atttcttgtc gggataatgt cagtgggagc tctggtagtt gcgtcatggc
    24481 tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct
    24541 tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacgaacc gagcatgctc
    24601 accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc
    24661 ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag
    24721 tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt
    24781 tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc
    24841 tttgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt
    24901 tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc
    24961 gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca
    25021 aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg
    25081 ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag
    25141 agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc
    25201 cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa
    25261 ccagtatctg aaccaacaag ggacgggtct gacccctgcc gagatgcatg gtttaatcag
    25321 tgggatgata tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac
    25381 gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc
    25441 caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga
    25501 tgatgtcagc gttttcgatc gggctgatgc actggcaggt tgggtcaatc acttcctgct
    25561 tggtcttggc gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat
    25621 cgacgatctg cgtaacatcg cgcagctggg ctacgacgaa gacgaagatc aggaagagct
    25681 tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga
    25741 cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa
    25801 acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc
    25861 gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag
    25921 aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact
    25981 tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata
    26041 accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc
    26101 gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg
    26161 aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac catgcccagg
    26221 gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt
    26281 cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa
    26341 tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga gaaatcaccg
    26401 ccatggcaca tacacgggcg atggaaaaat gccgtccggg aatgttcgag taccatctgg
    26461 aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccttcc tataacacca
    26521 ttgtaggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag agtgaactgc
    26581 gcgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac gcgggcgata
    26641 ttacccgcac cttcccggtc aacggcaaat tcacccaggc ccagcgtgaa atctacgaca
    26701 ttgtgctgga gtctctcgaa accagcctgc gcctgtatcg tccgggaact tccattctgg
    26761 aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcatcctga
    26821 aaggtaatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc
    26881 ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggtcag gatcgctcgc
    26941 gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc gcgccggatg
    27001 cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac attgtgatta
    27061 ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag
    27121 cgttgatggc tgctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg
    27181 cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg tacatttgat
    27241 tgaagcgact gcgccagagt cacatgctca tccgggcttt gatggacgag cgatagcgct
    27301 ggcggcgggt acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggctgattg
    27361 cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt catgctggat tcgtcaccct
    27421 cgccgcagaa gattaccaac tggcggcgct ggggcaggtt gtcgaattgc ataatgtcgg
    27481 gcaacggctg tttgcattgc tgcgaaaagc acctggcgta atgctgcatt gccccgatcg
    27541 cgtagctaac gttgcccgta ctcagagtca tgttgaagtg acgctggaga gcggcgagat
    27601 gctgacgggc cgcgtgctga tagccgctga tggcacccat tcagcgttag ccaccgcgtg
    27661 cggcgttgat tggcagcagg agccttacga acaactggca gtgattgcca acgttgctac
    27721 ttctgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc cgctggcgat
    27781 gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga
    27841 agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtctg cctttggctg
    27901 gcgactgggg caaattaccc acgccggtaa acgcagtgct tatccgctgg cattaacccg
    27961 cgccgccaga ccgattaccc atcgcactgt gctggtgggc aatgcggcgc aaactctgca
    28021 cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac
    28081 cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat tgtgccgcta
    28141 tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc gtcacggaca gcctggtaca
    28201 tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac tgatgacgat
    28261 ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg
    28321 ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg
    28381 gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac
    28441 tggagcagcg cgtaccggaa cctctggcgg tagatgcact accacaactg cgcgtttcgg
    28501 ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctcg
    28561 ctcgtagggc cagttgttat cacggcatgg aagtgtggga caaagacagt tttggtcaca
    28621 tctcgtttga cgatcaaagc atgggctata gccatcttgg gcacatcgtt gaaaactcag
    28681 tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc agatattact ctgttggccc
    28741 ccacagaatt acagcaggtc gcctggggag aaaatgaaac cttcctgacg ctgaaagatg
    28801 gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg tgctaattcc tggttgcgca
    28861 acaaagcaga tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca
    28921 ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc
    28981 tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag
    29041 aggaagcgca gcggatgcag caggcaagtg aagacgaatt taatcgcgcg ttaaatatcg
    29101 cttttgataa tcgccttggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccgctga
    29161 cggggcgtta tgcgcgccag tttgccgcac accgtctggc gctggtgggc gacgccgcgc
    29221 ataccattca cccactggcg gggcagggcg ttaatctcgg atttatggac gccgcagagc
    29281 tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc
    29341 tgcgccgcta tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct ggtatgcagg
    29401 gttttcgcga tctcttttcc ggcgctaatc cggcgaaaaa actgctgcgt gatattggtt
    29461 tgaaactggc cgacacgctt cctggcgtta agccgcaact tatccgccag gcaatgggat
    29521 taaacgattt gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta tttgataccc
    29581 atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa
    29641 tttcacctcc gttttcgcat tatattttct aataccatga ttttttgatt tagtgttttt
    29701 tgacattttt ttagctctta atattgtctt attcaaattg actttctcat cacatcatct
    29761 ttgtatagaa attggtgtat tttttggttt tttattctgt cgcgattttt gcatttttta
    29821 accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa
    29881 gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggttttg
    29941 tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag gacaagatgg
    30001 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    30061 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    30121 ccgatgccgg aatgtttgat gtgtcgcata tgaccatcgt cgatctccgc ggcagccgca
    30181 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    30241 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct
    30301 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    30361 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    30421 tttccatgat tgccgtgcaa gggccgaatg ctcaggcaaa agctgccaca ctgtttaatg
    30481 atgctcagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gtgggcgatc
    30541 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    30601 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg cgttaagccc tgcggtctgg
    30661 gggcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    30721 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccgacagatc
    30781 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    30841 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    30901 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    30961 gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg cgaaacggcg attgtgcaaa
    31021 ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    31081 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    31141 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    31201 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    31261 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga
    31321 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    31381 ggaactggtg aacagcgaac cgtatgcagg cggctggatc tttaaaatca aagccagcga
    31441 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagatga
    31501 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    31561 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    31621 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    31681 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg
    31741 cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gacaccaccg caggttggcg
    31801 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    31861 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    31921 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcag cctgaagtct
    31981 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    32041 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    32101 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccaatgtgc
    32161 atccgcaaac gctggatgtg gtccgtactc gtgctgaaac ctttggtttt gaagtgattg
    32221 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    32281 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    32341 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    32401 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    32461 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    32521 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    32581 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    32641 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    32701 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    32761 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    32821 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    32881 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagtgtgc
    32941 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    33001 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    33061 atcgctatca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    33121 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    33181 ccgagatgat cccaatcact tggacggaat ttgccgaact gcacccgttt tgcccgccgg
    33241 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    33301 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    33361 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    33421 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    33481 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    33541 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    33601 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    33661 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    33721 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    33781 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    33841 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    33901 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    33961 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    34021 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    34081 gcccgctgaa agaagaaacc ggcatcagcg agctggacat tgccaagcgc ctgatcgact
    34141 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    34201 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    34261 cagaaattga ccaggtgaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
    34321 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    34381 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    34441 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    34501 ctgattcgag tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    34561 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    34621 ggcactggcg cgaatattta ccctcacccc gaccctctcc ctgaaagggc gagggggaaa
    34681 agcgtgccaa cattgaagat tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa
    34741 ttaatttttc tcggaaaaac aaatggctat agcacttgtg accggtggca gtcgcggcat
    34801 cgggcgggca actgcattac tgttggcgca agaagggtat acggtggcgg ttaattatca
    34861 gcaaaacctc cacgcggcgc aggaagtgat gaacttaata atgcaagccg gtggcaaagc
    34921 attcgtgctc caggcggata tcagcgacga aaaccaggtc gttgcgatgt ttacagcaat
    34981 cgatcagcac gatgaaccgc tagcagcgct ggtcaataac gccgggatct tgtttaccca
    35041 gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga gtactttcca ccaacgtgac
    35101 gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa atggtggcag
    35161 tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg ttgggttcgc caggggaata
    35221 tgttgattac gcggcatcga aaggggcgat tgattcgtta accaccggac tatcgctgga
    35281 agtcgccgcg caggggatcc gcgttaactg cgtgcggcca gggtttattt ataccgaaat
    35341 gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca
    35401 gcgtggtgga caggcagaag aggtcgcgca ggccattgtc tggctactaa gtgataaagc
    35461 ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg aaataaaaca gggaagttgt
    35521 ctgaccggat gcaacaagta ttgcatccgg tacttcatcg acttaaagct tctcgccgtt
    35581 gctggcaatc acctctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc
    35641 agtaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt
    35701 agtgaatgac acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacaccatc
    35761 gtaagtcact gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc
    35821 gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat
    35881 aaacagcggc ctctgataac gctcatacag ttcgcaaagg gcatagcgca gacctactgg
    35941 atcaatctgc cagccccagt cagacgcttt aacatacggg ttcggtacgc tgccttcaaa
    36001 accagagatc gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct
    36061 gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg agatcgccgt cttccatttt
    36121 gatattaaat ccgcgacgtt cccactcgtt caatacatag gacgggtaat agccgcatag
    36181 ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc
    36241 cgggttacat gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc
    36301 cgggttaatg cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag
    36361 cacctggtac atcgtctctt ccgggttgtc atgctcggta tacaccacgc cggagcagca
    36421 gtaaccaaac agcggcgcac gccagttacg ctggttgttt atttcgttga aggtcatcca
    36481 gtatttgact ttatgcttgt agcgttcgaa taccacttcc gcgaaacgga caaagaaatc
    36541 aacgaattta cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa
    36601 gtgggagagg gttatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc
    36661 gtaaaacttc agcccttctt cgtttggctg agcttcatca ccttttggaa aaatacgcgt
    36721 ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc
    36781 cttgtagtga ccataaaaat caacggcttc atggtttgga tagtattttc ctggcaagac
    36841 ttctttggtg atttcgcgcg gcacgccgtg tgcgccacag gtcagaacgt cacaaatgct
    36901 cggtcctttg ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa cggcgccacc
    36961 ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca gatcagtcaa
    37021 ttcttacagg aaatagtacc atgcagccaa acgacatcac ttttttcaac gtttccagga
    37081 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    37141 aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga
    37201 agtcaccgca acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga
    37261 aaatatgacc ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca
    37321 attttatatg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    37381 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    37441 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    37501 ctctggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    37561 aaattgccaa cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt
    37621 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    37681 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    37741 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    37801 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    37861 tgattgttat ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc
    37921 gattcaaaat tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa
    37981 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg
    38041 tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca
    38101 tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
    38161 ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca
    38221 ggcatcggag cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt
    38281 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    38341 gcgataaatg cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg
    38401 atatgcagtg tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta
    38461 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    38521 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    38581 cggctggcac tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct
    38641 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    38701 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    38761 ccagcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    38821 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    38881 gggtgttcaa accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa
    38941 ggcagttcgc taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    39001 agcacacgct cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt
    39061 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg
    39121 cggaacagac gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg
    39181 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    39241 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    39301 aaacgggcag aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc
    39361 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    39421 ctttcactac gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa
    39481 ttgttgtgaa ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc
    39541 gaaaaacacg ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat
    39601 taacaacaaa gcccgaattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    39661 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    39721 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    39781 accggccgat gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg
    39841 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    39901 ttcatgggct ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt
    39961 tatggatggt gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta
    40021 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    40081 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    40141 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    40201 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    40261 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    40321 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    40381 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    40441 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    40501 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    40561 gcaataattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    40621 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    40681 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    40741 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    40801 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    40861 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    40921 tgaaacagga gctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    40981 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    41041 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    41101 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    41161 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    41221 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    41281 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    41341 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    41401 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt
    41461 taggggaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    41521 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    41581 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    41641 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    41701 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    41761 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    41821 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    41881 tttcaggctt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    41941 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    42001 gcggtgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    42061 ttagtggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    42121 ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta
    42181 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    42241 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    42301 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    42361 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    42421 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    42481 cgggttacca gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca
    42541 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac
    42601 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt
    42661 acgcagtgcg caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag
    42721 cggtgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat
    42781 tgtggtcggt gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat
    42841 gcgctcgctt ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta
    42901 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac
    42961 ggtggataac ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc
    43021 ggttattgtt accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat
    43081 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    43141 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    43201 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcactgg ggacagtagc
    43261 ggacgtcgtg ccgctggacg cgaataatcg cattctgacc tggcagggga tgagtcgcat
    43321 ccgtgccgga aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc
    43381 acaaaaacta gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg
    43441 gcgactggat gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggcgaagc
    43501 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    43561 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
    43621 cggcgggctg gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc
    43681 gcgtatcaaa gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac
    43741 gctgaaaggt tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    43801 agacacactc taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt
    43861 gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    43921 gtggctggcc ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    43981 cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    44041 cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg
    44101 tcatttgaag gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt
    44161 taatgtcgat accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa
    44221 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    44281 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    44341 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    44401 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    44461 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    44521 ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    44581 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    44641 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    44701 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    44761 actgttacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    44821 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    44881 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    44941 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt
    45001 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    45061 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    45121 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    45181 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    45241 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    45301 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    45361 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg
    45421 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    45481 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    45541 aactgaaaac gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga
    45601 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    45661 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    45721 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    45781 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    45841 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    45901 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    45961 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    46021 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    46081 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    46141 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    46201 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    46261 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    46321 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    46381 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    46441 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    46501 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    46561 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    46621 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    46681 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    46741 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    46801 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    46861 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    46921 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    46981 tcccggcgat gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    47041 ggggtttttt atttaagctg ggtaaatgca gataatcgta ttctggcttc gcgatttgtc
    47101 gcctgcatca ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca
    47161 tcagctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    47221 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgcctct cggatcggtg
    47281 gcgcggtagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    47341 taacggcaac ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa
    47401 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    47461 aattgcccct tggcattaaa cagccaactg gagaacgcga gatgtaagag ggtgtctgcc
    47521 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    47581 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    47641 tgaagagtga aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat
    47701 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    47761 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga
    47821 atatagcgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    47881 atcagaagcc acatacgtac tgtcctgact tctaaatctt tatcagagac ggattccttc
    47941 accaggtcag catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg
    48001 gttgcgagca taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag
    48061 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    48121 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    48181 atgccgaaca tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc
    48241 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    48301 cagcggctat gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg
    48361 gggaagctgt taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcaa
    48421 ataatatcgt gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc
    48481 atcgactcga taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg
    48541 aaaatcggca taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc
    48601 ccggataaat tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt
    48661 aatacggcga cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt
    48721 aagacggcaa aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct
    48781 ccagcggccc agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca
    48841 ccggtaacca gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg
    48901 gtgataaaac ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc
    48961 atcccaatgg ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg
    49021 cggatcccca taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa
    49081 aagagatccg agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca
    49141 atcataagag gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt
    49201 atgatcagct ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca
    49261 gaagatgagg gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag
    49321 aataaaaagc aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag
    49381 tttttgcgag gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt
    49441 aaaccacgaa aatgacaaga gaaactgaca atggaattat ctgtaatttt ctcatattga
    49501 tatttcatta atcattctgg ca
//
LOCUS       DAJVPT010000015        48999 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVPT010000015 DAJVPT010000000
VERSION     DAJVPT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1143)
                     /gene="setB"
                     /locus_tag="OQM47_000875"
     CDS             complement(<1..1143)
                     /gene="setB"
                     /locus_tag="OQM47_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1479134.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPTMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMII"
     gene            1511..2641
                     /gene="fruB"
                     /locus_tag="OQM47_000876"
     CDS             1511..2641
                     /gene="fruB"
                     /locus_tag="OQM47_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1479135.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2641..3579
                     /gene="fruK"
                     /locus_tag="OQM47_000877"
     CDS             2641..3579
                     /gene="fruK"
                     /locus_tag="OQM47_000877"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1479136.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3596..5287
                     /gene="fruA"
                     /locus_tag="OQM47_000878"
     CDS             3596..5287
                     /gene="fruA"
                     /locus_tag="OQM47_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1479137.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5711..6652
                     /locus_tag="OQM47_000879"
     CDS             5711..6652
                     /locus_tag="OQM47_000879"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1479138.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6640..7578
                     /gene="psuG"
                     /locus_tag="OQM47_000880"
     CDS             6640..7578
                     /gene="psuG"
                     /locus_tag="OQM47_000880"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1479139.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7672..8922
                     /locus_tag="OQM47_000881"
     CDS             7672..8922
                     /locus_tag="OQM47_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1479140.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8993..9687)
                     /gene="yeiL"
                     /locus_tag="OQM47_000882"
                     /pseudo
     CDS             complement(8993..9687)
                     /gene="yeiL"
                     /locus_tag="OQM47_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9818..10759
                     /gene="rihB"
                     /locus_tag="OQM47_000883"
     CDS             9818..10759
                     /gene="rihB"
                     /locus_tag="OQM47_000883"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1479141.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10859..12109
                     /locus_tag="OQM47_000884"
     CDS             10859..12109
                     /locus_tag="OQM47_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1479142.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12165..13253)
                     /locus_tag="OQM47_000885"
     CDS             complement(12165..13253)
                     /locus_tag="OQM47_000885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1479143.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13256..14113)
                     /gene="nfo"
                     /locus_tag="OQM47_000886"
     CDS             complement(13256..14113)
                     /gene="nfo"
                     /locus_tag="OQM47_000886"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1479144.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14187..15236)
                     /locus_tag="OQM47_000887"
     CDS             complement(14187..15236)
                     /locus_tag="OQM47_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1479145.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15335..16216
                     /locus_tag="OQM47_000888"
     CDS             15335..16216
                     /locus_tag="OQM47_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1479146.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16422..17891
                     /gene="lysP"
                     /locus_tag="OQM47_000889"
     CDS             16422..17891
                     /gene="lysP"
                     /locus_tag="OQM47_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1479147.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18183..20174
                     /gene="cirA"
                     /locus_tag="OQM47_000890"
                     /pseudo
     CDS             18183..20174
                     /gene="cirA"
                     /locus_tag="OQM47_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20206..21041)
                     /gene="yeiG"
                     /locus_tag="OQM47_000891"
                     /pseudo
     CDS             complement(20206..21041)
                     /gene="yeiG"
                     /locus_tag="OQM47_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21299..21967
                     /gene="folE"
                     /locus_tag="OQM47_000892"
     CDS             21299..21967
                     /gene="folE"
                     /locus_tag="OQM47_000892"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1479148.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21984..23060
                     /locus_tag="OQM47_000893"
     CDS             21984..23060
                     /locus_tag="OQM47_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1479149.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23202..24242
                     /gene="galS"
                     /locus_tag="OQM47_000894"
     CDS             23202..24242
                     /gene="galS"
                     /locus_tag="OQM47_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1479150.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24522..25520
                     /gene="mglB"
                     /locus_tag="OQM47_000895"
     CDS             24522..25520
                     /gene="mglB"
                     /locus_tag="OQM47_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1479151.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25581..27101
                     /gene="mglA"
                     /locus_tag="OQM47_000896"
     CDS             25581..27101
                     /gene="mglA"
                     /locus_tag="OQM47_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1479152.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27117..28127
                     /gene="mglC"
                     /locus_tag="OQM47_000897"
     CDS             27117..28127
                     /gene="mglC"
                     /locus_tag="OQM47_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1479153.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28163..29376)
                     /gene="preA"
                     /locus_tag="OQM47_000898"
                     /pseudo
     CDS             complement(28163..29376)
                     /gene="preA"
                     /locus_tag="OQM47_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29370..30608)
                     /gene="preT"
                     /locus_tag="OQM47_000899"
     CDS             complement(29370..30608)
                     /gene="preT"
                     /locus_tag="OQM47_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1479154.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30802..31041)
                     /locus_tag="OQM47_000900"
     CDS             complement(30802..31041)
                     /locus_tag="OQM47_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1479155.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31044..31763)
                     /gene="sanA"
                     /locus_tag="OQM47_000901"
     CDS             complement(31044..31763)
                     /gene="sanA"
                     /locus_tag="OQM47_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1479156.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31913..32797)
                     /gene="cdd"
                     /locus_tag="OQM47_000902"
     CDS             complement(31913..32797)
                     /gene="cdd"
                     /locus_tag="OQM47_000902"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1479157.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32927..33622)
                     /locus_tag="OQM47_000903"
     CDS             complement(32927..33622)
                     /locus_tag="OQM47_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1479158.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33619..34017)
                     /locus_tag="OQM47_000904"
     CDS             complement(33619..34017)
                     /locus_tag="OQM47_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1479159.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34256..35203
                     /gene="dusC"
                     /locus_tag="OQM47_000905"
     CDS             34256..35203
                     /gene="dusC"
                     /locus_tag="OQM47_000905"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1479160.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35574..35657)
                     /gene="yohP"
                     /locus_tag="OQM47_000906"
     CDS             complement(35574..35657)
                     /gene="yohP"
                     /locus_tag="OQM47_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1479161.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35881..37317
                     /gene="mdtQ"
                     /locus_tag="OQM47_000907"
                     /pseudo
     CDS             35881..37317
                     /gene="mdtQ"
                     /locus_tag="OQM47_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37370..38131
                     /locus_tag="OQM47_000908"
                     /pseudo
     CDS             37370..38131
                     /locus_tag="OQM47_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38248..38826)
                     /locus_tag="OQM47_000909"
     CDS             complement(38248..38826)
                     /locus_tag="OQM47_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1479162.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            38996..39583
                     /locus_tag="OQM47_000910"
     CDS             38996..39583
                     /locus_tag="OQM47_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1479163.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39757..40689
                     /gene="pbpG"
                     /locus_tag="OQM47_000911"
     CDS             39757..40689
                     /gene="pbpG"
                     /locus_tag="OQM47_000911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1479164.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40727..42442)
                     /gene="dld"
                     /locus_tag="OQM47_000912"
     CDS             complement(40727..42442)
                     /gene="dld"
                     /locus_tag="OQM47_000912"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1479165.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42638..44935
                     /gene="bglX"
                     /locus_tag="OQM47_000913"
     CDS             42638..44935
                     /gene="bglX"
                     /locus_tag="OQM47_000913"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1479166.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45187..46104
                     /gene="osmF"
                     /locus_tag="OQM47_000914"
     CDS             45187..46104
                     /gene="osmF"
                     /locus_tag="OQM47_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1479167.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46111..47268
                     /gene="yehY"
                     /locus_tag="OQM47_000915"
     CDS             46111..47268
                     /gene="yehY"
                     /locus_tag="OQM47_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1479168.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47261..48187
                     /gene="yehX"
                     /locus_tag="OQM47_000916"
     CDS             47261..48187
                     /gene="yehX"
                     /locus_tag="OQM47_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1479169.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48192..48923
                     /gene="yehW"
                     /locus_tag="OQM47_000917"
     CDS             48192..48923
                     /gene="yehW"
                     /locus_tag="OQM47_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1479170.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48904..>48999)
                     /locus_tag="OQM47_000918"
     CDS             complement(48904..>48999)
                     /locus_tag="OQM47_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1479171.1"
                     /translation="KIGVIALFLFMALGGIGGVMLAGYTFILRAG"
BASE COUNT        11831 a        12077 c        13160 g        11931 t
ORIGIN      
        1 gataatcatc accatcgcaa accagaacac agcgtgataa ttccagatct cggcgacgat
       61 gcccgccact gatcctgcga tgatccagcc cacgcgcgac gtgttggtat agagcgtggt
      121 ggctgaacct gcctgaccgg gcatcaaatc ctgaaaatag agcatcccga tgccgcccag
      181 aatgccaata aaaatagcat ttagcagctg caagcccaac agaatggcag gtgaatgcgc
      241 catcagcatt cctgcgtaaa aacagacgcc acccacggca gcaacgcgca ttaagaaacg
      301 cttacccaga cgtttggcga aatatccggc aatcaacatc gtcgggattt ccagcccggc
      361 ggcggtcccc atcatcacac cggccagttt ctcgggaaga tgcagttcgt tgataataaa
      421 tagcggcatg ttgatgatgt acaggctgtt cgagccccac atcaatgtac aaatgacaaa
      481 cagcagtagc gtatcgcgac ggttacggcg cggcgcttca accgtgccgg tcgccagcgg
      541 aagctctttt tgcatcgacg gtaaaaacag ccacaccatc acgccgcaaa caataaacgc
      601 taccgctgcg ctcagataca ttaccgtaaa gctgaaaccc atcgctaacg cataagccag
      661 cggtgggcca atgacccatg ccagtgaaac ctgagcgcgt aaaaaagagc tgaacatcac
      721 cgcctcacgt ccggttttgt cggcatgttc acgggcaagg gcaaacattt gcgggttagc
      781 ggtcgagcca aagctgctaa gaaagacgcc aacgaatagc aaaacaaagt agttgcgatt
      841 ccaagcaaaa agggtgcagg ccagcacgcc taacaggcag caaaagacaa tcagcgattt
      901 gcgatcgccg cgcttatcag agcgcccagc gagaaactga cttaccagaa tcccaatgac
      961 agcgctgccg gtaaagaaga atcccaccat cgtcggacgg gcatgtactt catcggtaag
     1021 gaaaatactg agtgtcgggg tttgcagagc acccgcaata ccggtgagaa aggcaacgat
     1081 taaaaacgcc gtcgaggtca ggtcaaacga tttcgcgctg gagactgcgg gggagttatg
     1141 catgctggtc gatcactgga agaggaagac gcggagttta cgctgcttgt cagaaaataa
     1201 acagacagcg aaatcaaaag cgccacgaat aatcaaaatg ccatttcact gttgctgatt
     1261 ggctgctgac gctgctgaaa tgaaattgct gataacggat tttcccatca gcaattagga
     1321 aaaatgacaa aaaattgtgc agcacatcaa acttttgctc ataactttac ggctttcctt
     1381 gcgtgctgaa acagaagtat tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa
     1441 ccctaagcta ggttggcgct ttttttctca tagaggctga atcgtttcaa ttcagcaaga
     1501 gaggagaact atgttccagt tatccgtaca ggacatccat ccgggcgaaa aggccggaga
     1561 caaagaagag gcgattcgcc aggtcgctgc ggcgctggtg caggccggta atgtagcaga
     1621 aggctacgtc aatggcatgc tggcgcgcga acagcaaacc tcaacgttcc tcggcaatgg
     1681 tattgctatt ccacacggca ctaccgacac ccgcgatcag gtgctgaaaa ccggcgttca
     1741 ggtatttcag ttcccggaag gcgtcacctg gggtgacggt caggtagcgt acgtggcaat
     1801 cggtattgct gccagctcgg ataaacatct gggcctgcta cgccagctga cccacgtact
     1861 gagcgatgat tccgttgctg aacaactgaa gtcagcaaca acagcagaag aacttcgcgc
     1921 attactgatg ggcgaaaagc agagtgagca gctgaagctc gacaacgaaa tgctgacgct
     1981 ggatatcgtc gccagcgatc tgctgactct tcaggcgctg aacgctgcgc gtctgaaaga
     2041 ggcgagggca gttgacgcca ctttcgtcac caaagccatc aatgaacaac cgctgaatct
     2101 cggacagggt atctggctga gcgatagcgc cgaaggcaat ctgcgtagtg cgattgcggt
     2161 aagccgtgcg gcaaatgctt ttgatgtgga cggcgaaacg gcagccatgc tggtgagtgt
     2221 ggcgatgaat gacgatcagc ccatcgcggt tcttaagcgt ctcgctgatt tgttgctcga
     2281 caataaagct gaccgcttgc tgaaagcgga tgcggcaacg ttgctggcgc tgctgaccag
     2341 cgatgatgcg ccgaccgacg acgtgttaag cgcggagttt gtggtgcgca atgaacacgg
     2401 cctgcatgct cgtccaggta ccatgctggt caataccatt aaacaattta acagtgatat
     2461 taccgtgaca aaccttgatg gcaccggcaa accggcaaac ggacgtagtc tgatgaaagt
     2521 tgtggcactt ggcgttaaga aaggtcatcg cctacgcttt accgcccagg gtgcagatgc
     2581 tgaacaggcg ctgaaagcca ttggcgacgc tatcgctgct ggtcttgggg agggcgcata
     2641 atgagcagac gtgttgctac gatcaccctt aatccggctt atgaccttgt tggtttctgc
     2701 ccggaaattg aacgcggcga agtgaacctg gtgaaaacca ccggtctgca tgcggcgggt
     2761 aaaggcatca acgtggccaa agtattaaaa gacctgggaa ttgatgtcac cgttggcggc
     2821 ttcctgggta aagacaatca ggatggtttt cagcaactgt tcagcgagct gggcattgcc
     2881 aaccgtttcc aggttgtaca ggggcgcacc cgaattaacg ttaagctgac ggaaaaagac
     2941 ggcgaagtga ccgacttcaa cttctcgggt ttcgaagtca cccccgccga ctgggaacgc
     3001 tttgtgactg attctctgag ctggctcggt cagttcgata tggtctgtgt cagcggaagc
     3061 ttaccgtcag gcgtcagccc ggaagcgttc accgactgga tgactcgcct gcgtagtcag
     3121 tgtccttgca ttatctttga tagtagccgt gaagcgttag tagcaggttt gaaagcggca
     3181 ccgtggctgg tgaaacctaa ccgccgcgag ttggaaatct gggcaggccg taaactgcct
     3241 gaaatgaaag atgtgattga agctgcacat gcgctacgtg aacaaggtat tgcgcatgtt
     3301 gttatttcac tgggtgccga aggcgcgctt tgggttaatg cctccggcga atggatcgct
     3361 aaaccaccgt cagtcgatgt cgtaagcacc gttggcgcag gggattctat ggttggtggc
     3421 ctgatttatg gcttgctgat gcgtgaatcc agtgaacaca cactgcgtct ggcgacagct
     3481 gttgcagccc tggcggtaag tcaaagcaat gtgggtatta ccgatcgtcc gcagttggcc
     3541 gcaatgatgg cgcgcgtcga cttacaacct tttaactgac agcaggagag gcataatgaa
     3601 aacgctgctg attattgacg ctaatctcgg tcaggcacgc gcctatatgg cgaagaccct
     3661 gctgggcgcg gcggcgcgaa aagcaaaact ggaaatcatc gacaatccga acgacgcaga
     3721 aatggcgatt gttctcggtg attccatccc gaatgacagc gcgctgaacg gtaaaaatgt
     3781 ctggctgggt gatatttccc gggcagttgc gcaccctgag ctgttcctga gtgaagccaa
     3841 aggccatgcg aaaccttaca ctgcgccggt cactgcgaca gcaccggttg ccgccagcgg
     3901 tccgaaacgc gtagttgcgg tgactgcttg cccgactggc gtagcacaca cctttatggc
     3961 ggctgaagcc attgaaaccg aagcgaaaaa acgtggctgg tgggtgaaag ttgaaacccg
     4021 tggttctgtt ggcgcgggta atgcaatcac tcccgaggaa gtagccgcag cggatctggt
     4081 gattgtggcg gcagatatcg aagtggatct ggcgaaattt gctggtaaac cgatgtatcg
     4141 tacctctacc ggtctggcgc tgaagaaaac ctcgcaggaa ctggataaag cggttgctga
     4201 agcaacgccg tatgaaccgg cgggcaaagc tcaaacggcg accactgaag gtaagaaaga
     4261 gagtgcaggc gcttatcgtc acttgctaac gggcgtctct tacatgctgc cgatggtcgt
     4321 tgctggtggt ctgtgtatcg cgctttcttt tgcttttggt atcgaagcgt ttaaagagcc
     4381 gggtacgttg gcagcggcgc tgatgcagat tggtggtggt tcagcctttg cgctgatggt
     4441 gccggtactg gcaggttata ttgccttctc cattgccgat cgtccgggcc tcacgccggg
     4501 tctgattggc ggtatgctgg cggtcagcac cggttctggc ttcattggcg gtattattgc
     4561 gggcttcctg gctggttaca ttgcgaagtt aatcagtacg caactgaaac tgccgcagag
     4621 tatggaggcg ctgaaaccga tcctgatcat tccgctaatt tccagtctgg tggtcggtct
     4681 ggcgatgatc tacctgatcg gtaaaccagt tgctggcatt ctcgaagggc tgactcactg
     4741 gctgcagacc atggggactg cgaatgcggt tctgctgggg gcgatcctcg gtggcatgat
     4801 gtgtactgac atgggcggtc cggtaaacaa agcagcgtac gcattcggtg tgggtctgct
     4861 gagtactcaa acctatggcc cgatggcggc gattatggcg gcaggtatgg tgccaccgct
     4921 ggcaatgggc ctggcaacaa tggtggcgcg tcgcaaattc gacaaagcgc agcaggaagg
     4981 tggcaaagcc gctctggtat tgggactgtg cttcatttcg gaaggtgcaa ttccgtttgc
     5041 tgctcgtgat ccgatgcgtg tgctgccgtg ctgtatcgtg ggtggggcgc tgactggcgc
     5101 aatctcaatg gcgattggtg cgaaactgat ggcaccgcac ggtggtctgt ttgttctgct
     5161 gatccctggc gctattacgc cggtattggg ttacctggta gcaattattg ccggtacgct
     5221 ggtggcgggt ttggcctatg ccttcctgaa acgtccggaa gtggacgcag tagcgaaagc
     5281 agcgtaataa aaggtgtgtt acagggcaga aatttcctgc cctgtaacat ttgatgagcg
     5341 caaagagaat gaccagcttg tgaatgtaat tttatgaatt taaatggttt tatctttcag
     5401 tagaaacttc aaacgttcat caaacactaa ggttatttcc tcaagggcag attattttct
     5461 gccctttttt agtgttcctg ttctgtgcat cccgtcacaa attcctcgct tttgtaattc
     5521 aggcatttaa tcgcattttg tgagcctgct caaatctgcc cattttgcct ctctggcggt
     5581 aatttacttt acgtaaaaca tgcatactat gagcacatgt ttaaatatga acacatgttt
     5641 aaaccgtctt tgcagtatct atattcttgt ggattgacag ggttggtaat tttttaccag
     5701 aggacgtgct atgcgcgaaa aggattatgt cgtaattata ggttcggcga atattgatgt
     5761 cgccggatat tcacatgaat cattaaatta tgcggattca aatccaggta aaataaaatt
     5821 tacgcctggt ggagtagggc gcaatattgc acaaaacctg gcgttgctgg gtaacaaagc
     5881 ctggctactg agcgccgtag gcagtgattt ttatggtcaa tcgctgctaa cgcaaaccaa
     5941 tcaatctggt gtttatgtcg ataaatgcct gattgtgccg ggagaaaata cgtcgagtta
     6001 tttatcatta ctcgataata ccggtgaaat gctggttgct ataaatgaca tgaatattag
     6061 caacgctatt acagctgaat atctcgcaca gcaccgtgaa tttattcaga gggcaaaggt
     6121 cattgtcgcg gactgtaata tcagtgaaga ggcactggca tggattctgg ataatgccgc
     6181 caacgtaccc gtatttgtcg atccggtttc agcgtggaaa tgtgtcaaag tgcgcgaccg
     6241 tctaaatcag aaccacactc tgaagccaaa ccgccttgaa gcggaaaccc tgagtgggat
     6301 tgcgctgtca gggcgtgacg atgtggcaaa agttgctgcc tggttccatc aacatggcct
     6361 gaaccgactg gtattgagca tgggcggcga cggcgtttat tacagcgata tcagcggtga
     6421 aaatggctgg tctgcgccga tcaaaaccaa tgttattaat gttaccggag cgggcgatgc
     6481 catgatggcg ggacttgctt cgtgttgggt agacggaatg ccgtttgccg aatctgttcg
     6541 tttcgcacag ggatgttcgt caatggcgct ctcctgtgaa tacaccaata accccgattt
     6601 atcgattgcc aacgttatat cgttagtgga gaacgcagaa tgtctgaatt aaaaatttcc
     6661 cctgaattat tacaaatttc tccggaagtg caggacgctt taaaaaacaa aaaaccggtt
     6721 gtggcgctgg aatcgaccat tatttctcac gggatgccgt tcccacaaaa tgcccagacc
     6781 gcaattgaag tagaagaaac tattcgtaaa cagggcgcag tacctgccac tatcgccatt
     6841 attggcggcg tgatgaaagt gggtttaagc aaagaaaaaa ttgaattact gggtcgtgaa
     6901 gggcataacg tgaccaaagt tagtcgtcgc gatttacctt ttgttgttgc cgccggaaaa
     6961 aatggcgcaa ccactgtggc ttcaacgatg attattgcgg cgcttgccgg aattaaagta
     7021 tttgccaccg ggggaattgg tggtgtgcat cgcggggcgg aacatacctt cgatatttct
     7081 gccgatttgc aagaactggc aaatactaat gtcaccgttg tttgtgccgg ggcgaaatct
     7141 attctcgatt taggattaac cactgagtat ttagaaacct tcggtgtgcc gttaattggc
     7201 tatcagacta aagcgctgcc tgcgtttttc tgccgtacca gctcgtttga cgtcagcatt
     7261 cgtctcgaca gcgccagtga aattgcccgt gcaatggcgg tgaaatggca aagcgggctg
     7321 aacggtggcc tcgtggtagc gaacccgatc ccggaacagt ttgcgatgcc ggaggaatct
     7381 atcaatgcag ccatagatca agccgtcgcc gaagccgaag agcagggcgt tattggtaaa
     7441 gaaagtacac cgttcctgct ggcgcgcgtt gctgagctga ccagcggtga cagcctgaaa
     7501 tccaatatcc agctggtgtt caacaacgcc attctggcga gtgaaattgc caaagaatac
     7561 cagcgtctcg cgggttaatt tctgacgggc agaaagccgt cgcccgtact catcaagcat
     7621 accaaaacct ggcgctgacg ccgggtttac tggcatgaag ggaaaatcat tatggatata
     7681 atgagaagtg ttgtggggat ggtggtgtta ctggcaatag catttctgtt gtcagtgaat
     7741 aaaaagagca tcagtttgcg cacggttgga gccgcactgc tgctgcaaat cgctattggt
     7801 ggcatcatgc tctacttccc accgggaaaa tgggcagtag aacaggcggc attaggcgtt
     7861 cataaagtga tgtcttacag tgatgccggt agcgccttca tttttggttc gctggttggg
     7921 ccgaaaatgg atgtcctgtt tgacggtgcg ggttttatct tcgcctttcg cgtacttccg
     7981 gcgattattt tcgttactgc gctcatcagt ctgctgtact acattggcgt gatggggctg
     8041 ctgattcgca tccttggcag cattttccag aaagccctca acatcagcaa aatcgaatct
     8101 tttgttgcgg ttaccactat tttcctcggg caaaatgaga tcccggcgat cgttaaaccg
     8161 tttatcgatc gcgtgaatcg caacgagttg tttaccgcaa tttgtagcgg gatggcgtcc
     8221 attgctggtt cggtgatgat tggttatgcc ggaatgggcg taccaattga ctacctgtta
     8281 gcggcatcgc tgatggcgat ccccggcggt attttgtttg cacgtattct tagcccggca
     8341 accgagcctt cgcaggtcac atttgaaaat ctgtcgttca gcgaaacgcc gccaaaaagc
     8401 tttatcgaag cggcggcgag cggtgcgatg accgggctga aaatcgccgc tggtgtagcg
     8461 acggtggtaa tggcgtttgt cgcaattatt gcgctgatca acggcattat cggcggaatt
     8521 ggcggctggt ttggtttcgc caatgcctct ctggaaagta tttttggcta tgtgctggca
     8581 ccgctggcgt ggatcatggg tgtggactgg agtgatgcca accttgctgg tagcctgatt
     8641 gggcagaaac tggcgattaa cgaattcgtc gcttacctga gtttctcccc atacctgcaa
     8701 acgggcggca cgctggaagt gaaaaccgtg gcgattatat cctttgcgct ttgtggtttt
     8761 gctaactttg gttctatcgg tgttgtcgtt ggcgcatttt cggctatttc gccaaaacgt
     8821 gctccagaaa tcgcccagct tggtttacgg gcgctggcag cagcaacgct ttccaacctg
     8881 atgagtgcga ctattgccgg gttctttatt ggtctggcgt aatgctcgat ttgtagaccg
     8941 gataaaacgt attaagcgtt gcatccggca atcgcgctat tgcaaataat ttttactgca
     9001 tcatcccgga gaatttattc tccgggtcca tctccagcgc cagtcctgac aactgctttc
     9061 tgtttttaat tagatatcct ttcttttaat taataaaccg tcgtgaatga attgcgcgag
     9121 aacatataaa agatgtcgat aagaaacgcc taaatactct gccgcttgcg tatgcttttc
     9181 gtgataaaga tcaccttctt gtgagagtaa tataaatgct gccaggcgat tcactaacgg
     9241 aaatgattga ttctgagtta aagaaacaat attacgataa tttttatgac ttaaggtgac
     9301 gcaaagtttt cgtaaaaata gggtgtcgtt taataacagc ggacggtaat gtttcatagg
     9361 gagcgcaagg caccaacact cttcaatagc ctgcaccgca cgcggttcat ggtctttgtc
     9421 gattaactca atctcgccaa taaaacaggg agcggcaaag aaatcgatca gcgacacgcg
     9481 accattggca agcgtggcgt aaagcctggc gcggcctcgc gtcagataaa acagccatga
     9541 cggctgttgc ccttcctgca caatgtagtc acgcgctaaa aaatgaaaca gccgcgtatc
     9601 ggctgaaaca tccgttgaaa agcaatcctt aaacgcagat tcactcatca attgctgctt
     9661 aaggtcatta ttgtggattt ctttcacgat gtttatccgc tatgagattt ctcatattta
     9721 gcatacgtac gctgttgtta agattcaatc attggcccgg tcgaattttc tttgctgtag
     9781 aaaaaaataa cgcaactgga aacagaggaa ataaacaatg gaaaagagaa aaattattct
     9841 ggattgtgag ccgggtcatg atgatgctat tgcaatgatg atggcagcga aacatccggc
     9901 aatagattta ttaggcatca ctattgtagc gggtaatcag acgcttgata aaacattaat
     9961 taatggcctg aatgtttgcc agaaactgga gattaatgtt ccggtttatg cggggatgcc
    10021 gcaacccatt atgcgtaaac aaatcgttgc cgataatatt cacggtgaaa ccggactgga
    10081 tggcccggta ttcgagccgc tgacccgcca ggcagaaagt acccatgcgg tgaaatatat
    10141 catcgatacc ctgatggcaa gcgatggcga tatcactctg gtgccggttg gtccgctttc
    10201 aaatatcgcg gtggcaatgc gtatgcaacc cgcaatcctg cccaaaatcc gtgaaattgt
    10261 gctaatgggc ggcgcttacg gtacaggcaa cttcacccca tctgccgagt tcaatatctt
    10321 tgccgacccg gaagccgccc gcgtggtgtt cacctccggc gttccattag tgatgatggg
    10381 cctcgatctt accaaccaga ccgtttgcac cccggatgta attgctcgga tggaaagggt
    10441 aggcggccct gccggagagc tgttcagcga catcatgaac ttcactctca aaacgcagtt
    10501 cgaatactac ggcctggctg gcggcccggt gcatgacgcc acctgcattg gttatctgat
    10561 caaccctgat ggcattaaaa cccaggatat gtacgtcgaa gtggacgtca acagcggccc
    10621 ttgctacggg cgtacggtct gcgacgaact gggcgttctt ggcaagcccg ctaataccaa
    10681 agtcggcatc actattgata ccgactggtt ctggggctta gtcgaagagt gcgtgcgcgg
    10741 ctacatcaaa acccattaag tgttgataac aagccgggac agtatcgcaa gatgcaggcc
    10801 cggctatttc tgatgggcag aaagccgtcg cccaaaatga catgaagaga taatcactat
    10861 ggatgtcatg agaagtgttc tgggaatggt ggtactgctg gcaattgcgt ttttactgtc
    10921 agtaaacaag aagaagatca gcctgcgtac cgttggcgcg gcgttagtgt tacaggtcgt
    10981 gattggcggg attatgcttt ggttaccgcc agggcgttgg gtcgcagaaa aagtcgcttt
    11041 tggcgtccac aaagtgatgg cgtacagcga cgcaggtagc gcatttatct tcggttctct
    11101 ggtcggaccg aaaatggata ccttatttga tggcgcagga tttatctttg gtttcagggt
    11161 attaccggca attatcttcg tcactgcact ggtgagtatt ctctactaca tcggtgtgat
    11221 ggggatctta attcgcattc tcggcggtat cttccagaaa gctttaaata tcagcaagat
    11281 cgagtcattc gtcgcggtca ccaccatttt cctcgggcaa aacgaaattc cggcgatcgt
    11341 caaacccttt atcgatcgtc tgaatcgcaa tgaattattt acagcgattt gtagtggcat
    11401 ggcctcgatt gctggttcga caatgattgg ttacgccgca ctgggcgtac ctgtggaata
    11461 tttgctggcg gcatcgttaa tggcgatccc tggcgggatc ttgtttgccc gcctgttaag
    11521 cccggctacg gaatcttcgc aggtttcttt taataacctc tctttcaccg aaacaccgcc
    11581 aaaaagcatt attgaagccg atgcgacagg ggcaatgacc gggctgaaaa tcgccgccgg
    11641 tgtagcgaca gttgttatgg catttgtcgc catcattgcg ttaattaacg gtattatcgg
    11701 cggcgttggc ggctggtttg gttttgaaca tgcctcgctg gagtccattt taggttacct
    11761 gctggcccca cttgcgtggg taatgggtgt tgactggagt gatgcaaatc ttgccgggag
    11821 tttgattgga cagaaactgg caataaatga atttgtcgct tatctcaatt tctcacccta
    11881 tctgcaaacg gctggcactc tggatgctaa aaccgtggcg attatttcct tcgcgttgtg
    11941 cggtttcgct aactttggtt ctatcggggt ggtggtgggg gcgttttctg cggttgcgcc
    12001 acaccgtgcg ccggaaatcg cccagcttgg tttacgcgcg ctggcggcgg cgacactttc
    12061 taatctgatg agtgccacca ttgccgggtt ctttattggt ttagcgtagt gtgcaattta
    12121 tgagccggat tagatgcggt caagcgtcga atccggcaac attgttacac cattggcact
    12181 aatgacagcg cgttatcggc agacagggta gggttgttta agctgccgct ggcgcgtgaa
    12241 attgccgcgc aggccatcgc aaaacgggcg ctgtcacgga aattgtttcc ctccagaaag
    12301 ctatatacca gacccgccat aaaaccatcg tcagcaccaa aactgtctac cgtcgtatgc
    12361 gctggcacag tcagcaaaaa ttgttcacca tccttttcgc tgcaataaac tgactcatcg
    12421 ggcaaataaa caaacagttg ctgaacacct tgctgatgca atgcattcac tgcggcatta
    12481 cggtcagcat cgctggtgat cgcctgtccc cataaaattt ccagctccgg taaagtgggt
    12541 ttcagggtgt gaatatgcgc cagccagtgt ttgattttgc ccgctttgaa ttctgaaacg
    12601 gtatcgacaa acaccgggat ctcatcagcg agggtaaaga cccattccag cgcctcggct
    12661 gtcaggttgc aatcagccag taccacgccc gcatgacgaa gtaaatcgcg cgacccgttc
    12721 agtagttgcg gtgtcaactg ttccagcaga tgggtatcgt taattgccag cacggtttga
    12781 tcgtctcgat tggcaattgc cagatacgtc gatgtgcttt gaccatgcaa acgaacgcag
    12841 ccggagacat tcacgccggc acggcgcgtt tcttccagga gcatttcgcc ataaaagtca
    12901 tcgccaatca ctgaaagcaa atggacgtca cggcctaaca gcgccagatt gtgggcgata
    12961 ttgcgtccaa cgccgcctgc cgaacaatga attgtaccgg gatgagaagc cgcttgcggg
    13021 taatggatat ccgccatccc gcgaatatcc atattgattg tccccaccac tacgcagtat
    13081 tcctgctcgg tgagaatgta acctttgtct ttaatccggc ctttgcgcat taaatccata
    13141 atatgcgccg caacgcgcga acgactgatt tgcagcatgt ccgcaatttc gttctgctga
    13201 atcagcgggt tacgccgtaa aattgcaagg atctcctttt cccggttatt catcttcagg
    13261 ctaccgcttt ttcagtttgt tgcgctttca gccaggcgat ctcttctgcc cagatatccg
    13321 ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg gtcgttctgc ataatccagc
    13381 ggaacgcatc atgaccgata ttgccttcac cgaggctatg atggcggtca acgcggctgc
    13441 caaaggtact tttcgcatcg ttaaggtgca tcccgcgcaa atacttaaag ccgacaatac
    13501 gggcaaaatc agcgaacgtt ttttcgcatt cggctggagt acgcaaatca tacccggcag
    13561 cgaaagcatg gcaggtatca atgcagacgc cgacgcggga tttatcttcc acgccgtcga
    13621 taatcgccgc gagatgttcg aatttaaacc ctaagttact gccctgaccg gcggtgtttt
    13681 caatcaccgc tgttacacct tgagttttat ccagcgcaat gttgatggat tcggcaatac
    13741 gcgcaaggca atcctcttct gaaatctgca tcagatggct gccagggtgg aagttgagca
    13801 aagaaagccc cagttgttcg caacgctgca tttcatctat aaaggcatcg cgcgattttt
    13861 ccagagcttc agtgaccgga tgtccgaggt tgatcagata actgtcgtgg ggaagaattt
    13921 gcgccgatgt gtagtgatat ttttcacagg cggctttgaa ttcatcgatg gtttgcgtcg
    13981 tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa caaggcaaac gcggttgcgt
    14041 cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc agcagcacta acgtgcgctc
    14101 caatgtattt catgcgagga ctcctgttaa acccgctgga ggaaaacggt aatgatagcg
    14161 ggttaacagg agcaagaagt agtggtttat gcgatgacgc tttgaatcac atagttaatc
    14221 gcaccaccac caacaatcag ccaggcaaac agtaccagtg ccatcagcag aggtttcgcc
    14281 ccagcttttt tcagcgcgct gacgtgagtg gtcagaccca gcgccgccat cgccattgcc
    14341 agcaggaagg tatccagcgt taccagcatg ttcaccacgc tctgcggtaa caggtggaac
    14401 gagttaaaga tggcaactac gatgaacaag atggcaaacc acggaatagt gattttgctt
    14461 ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg ccgccagcag gatgaggaac
    14521 ggagccagca tcatcacgcg cagcattttg gaaataactg ctgcgttttc tgcatccggg
    14581 ctgatggcat gacctgccgc caccacctgc gccacttcgt gcacagtaga accaatgtag
    14641 ataccgaaag tttccggact aaaccattga gacatcagcg gatatatcgc cgggtagagg
    14701 aaaatcgcga cggtcccgaa gataacaacg gttgcaacag ccacggttac tttactggct
    14761 tccgctttca ctaccggctc agtcgccagt accgcggcag caccacagat actgctaccg
    14821 gcaccgatca accaactggt gtgcttatcc agaccaaaca ctttctgccc caggaagcaa
    14881 gccagcagga aggtactgga cagcgtcagc acgtcaatga tgatcccact gataccgaca
    14941 tcggcaattt gcgagaacgt cagacggaag ccataaagaa tgatacccag acgtaataaa
    15001 tattgcttgg caaacagcac accaccgtca cagcttttcc agatgtgcgg atagatggtg
    15061 ttgcctaaaa ccatccccaa caagattgcg agggtgaggg cactaaaccc ggcacccgca
    15121 accgcgggaa tggaaccacc ccacagggcg accccggtga taactgcact cagggctaac
    15181 cccggaataa aatgccacag tgtacgatgt tgtttctgta aggtgatatt cgtcataacc
    15241 ctctccttta accgggcata aggttacgac taactggttt aaaaataaaa ttgattatat
    15301 atttataatt aatctttata agtggtaagc gactatgcac atcaccctcc ggcagttgga
    15361 agtttttgca gaagtattga aaagtggatc aaccacccag gcgtcggtga tgctggcgtt
    15421 gtcgcaatca gcagtgagcg cagccttgac tgacctggaa gggcagcttg gcgttcaact
    15481 gtttgatcgc gtggggaaaa gactggttgt taatgaacac gggcggctgc tctatccgcg
    15541 agcgttggca ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg aagacaacgg
    15601 cgcgattcgt atctatgcca gtagtaccat cggtaactac attctgcctg cagttatcgc
    15661 ccgttatcgc catgattatc cgcagttgcc gattgaactt agcgttggga acagccagga
    15721 cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc tttattgaag gaccgtgcca
    15781 cagcactgaa atcatttctg aaccgtggct ggaagacgag ctggtggttt tcgccgcgcc
    15841 gacttcgccg ttggctcgtg gtccggtcac cttagaacag ctggccgctg cgccgtggat
    15901 cctgcgtgaa cgcggttccg gcacgcggga tattgtcgat tatctgttgc tgtcacattt
    15961 accgaagttt gagatggcga tggaattagg taactccgag gcaatcaaac atgcggtgcg
    16021 tcatgggttg ggaattagtt gcctgtcgcg acgtgtgatt gaagatcaat tgcaggcagg
    16081 cacattaagt gaagttgcgg tccctctgcc gcgcctgatg cgtacgttgt ggcgtataca
    16141 tcatcggcaa aaacaccttt ccaacgcgct acggcgcttt ctggactatt gcgatcccgc
    16201 aaatgtgccg cgttaagttg ctgtacaaga acatgctggt gctgtgtcga tttcgtgacg
    16261 cagcgccttc agcatgcatt cgccagaaaa gagattggct gctttactta taatccctgg
    16321 gcgatcatga aggtgtctta taaccgtgta tttctgccgg aaggattgcc aatcgtctgc
    16381 tacaatcgcg ccatcatttt taagatggat agcatttttg tatgggttcc gaaactaaaa
    16441 ctacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg atgattgcca
    16501 ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt tctcaggcag
    16561 gtccgggcgg ggcattgctc tcgtatatgc tgattggcct gatggtttac ttcctgatga
    16621 ccagtctcgg tgaattggct gcatatatgc cggtttccgg ttcgtttgcc acttacggtc
    16681 agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg tacaactggg
    16741 cggtgactat tgccgttgac ctggttgcag cacagctggt catgagctgg tggttcccgg
    16801 atacaccggg ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg ttgaactaca
    16861 tctctgttcg cggctttggt gaagcggaat actggttctc actgatcaaa gtcacgacag
    16921 ttattgtctt tatcatcgtt ggtgtgctga tgattatcgg tatcttcaaa ggcgcgcagc
    16981 ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt tttgcggcga
    17041 tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg atcggtattg
    17101 ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt caggtattct
    17161 ggcgaatcct gttgttctat gtgttcgcga tcctgattat cagcctgatt attccgtata
    17221 ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgtcagtccg ttcaccctgg
    17281 tgttccagca cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt attctgacgg
    17341 cggtgctgtc agcaggtaac tccggtatgt atgcgtctac tcgtatgctg tacaccctgg
    17401 cgtgtgacgg taaagcgccg cgcattttcg ctaaattgtc gcgtggtggc gtgccgcgta
    17461 atgcgctgta tgcgacgacg gtgattgccg gtttgtgctt cctgacctcc atgtttggca
    17521 accagacggt atacctgtgg ctgctgaaca cctccgggat gacgggtttt atcgcctggc
    17581 tggggattgc cattagccac tatcgcttcc gtcgcggtta cgtattgcag ggacacgaca
    17641 ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc gcattcattc
    17701 tgtgtctgat tatcactttg ggccagaact acgaagcgtt cctgaaagat accattgact
    17761 ggggcggcgt agcggcaacg tatattggta tcccgctgtt tctgattatt tggttcggct
    17821 acaagctgat taaaggaact cacttcgtac gctacagcga aatgaagttc ccgcagaacg
    17881 ataagaaata agtttcctcc cttccttgct aagccctctc aaccgagagg gctttttcaa
    17941 ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg ataattgtta
    18001 tcgtttgcat tatcgttacg acgcaatcaa aaaaggctga caaatcagag gctgttccgg
    18061 ctttctggga tgatcacctg catacaaata agtccaccgc gatgctgccg tacgcaaggg
    18121 gacgtaaaga agatgtgagc gatacccatt ttattttcgt agttacctca tggagatatg
    18181 gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg tttgtccgct atttcttgtg
    18241 catggcctgt gttagcggtc gatgatgatg gcgaaacgat ggttgtcact gcatcttccg
    18301 tggaacaaaa tcttaaagat gcacccgcca gtatcagcgt cattacccag gaagacctgc
    18361 agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga agtgcctggc gtacaactga
    18421 cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg tctggacagc agctacaccc
    18481 tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc cgtcttccgc cacaatgatt
    18541 tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat tgaagtggtt cgtggcccga
    18601 tgtcgtcgct atacggctcc gatgcgctcg gcggtgtagt gaatatcatc accaaaaaaa
    18661 tcggtcagaa atggtcgggt accgttaccg tcgataccac cattcaggaa catcgcgatc
    18721 gcggtgatac ctataacggt cagttcttta ccagtggacc attaattgat ggtgtgctgg
    18781 gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga tgacccgcaa aactcaacga
    18841 ccaccgatac cggagaaacg ccgcgtattg aaggattctc cagccgcgac ggcaatgtcg
    18901 aatttgccta gacaccgaat caaaatcacg attttactgc cggatacggt ttcgaccgtc
    18961 aggatcgtga ttccgattcg ctggacaaaa accgcctgga acgccagaac tactgcgtca
    19021 gccataatgg gcgttgggat tacggcacca gcgaactgaa atcctacggt gagaaagtcg
    19081 agaacaaaaa ccctggcaac agcagcccga taacttccga aagcaatacg gtcgacggca
    19141 aatacacgtt gccgctgacg gcgattaatc agtttctcac ggttggcggt gaatggcgtc
    19201 acgacaaact tagcgatgcg gtgaacctga ccgggggaac cagctccaaa acgtctgcca
    19261 gccagtacgc gctgtttgtg gaagatgaat ggcggatctt cgagccgctg gcgctgacga
    19321 ccggcgtgcg tatggacgat cacgaaacct acggtgaaca ctggagtccg cgtgcctaca
    19381 tggtttataa cgccaccgac accgtaacgg tgaaaggggg ctgggcgacg gcatttaaag
    19441 caccttctct gttgcaactt agccctgact ggacgagcaa ttcctgccgt ggcgcatgta
    19501 agattgtggg tagcccggat ctgaaaccag aaaccagcga aagttgggag ctggggcttt
    19561 actacatggg tgaagaaggc tggctggaag ggggtgaatc cagcgttatc gttttccgta
    19621 acgatgtgaa agatcgtatc agcatcagcc gtacgtctga cgtcaacgct gcaccgggct
    19681 accaaaactt tgttggtttt gagacgggcg ctaacggacg gcgcataccg gtatttagct
    19741 actacagcgt taacaaagct cgtattcagg gcgtggaaac cgaactgaaa attccgttca
    19801 acgatgaatg gaaactgtcg atcaactaca cctacaacga tggtcgtgat gtcagcaacg
    19861 gcgaaaacaa accgctatcc gatctgccgt tccatactgc taacggtacg ctggactgga
    19921 aaccgctggc gctggaagac tggtcattct atgtttctgg tcactatacc gggcagaaac
    19981 gcgccgacag cgcgacggct aaaacaccgg gcggttatac catctggaat accggcgcgg
    20041 cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt gctgaacctt ggcgacaagg
    20101 atctcagtcg tgacgactac agctataacg aagacggacg tcgttacttt atggcagtgg
    20161 attatcgctt ctgatgagaa gatgcccggc gaaccgggcg gactttcact tcagtaaata
    20221 ctgcgcatgg aagcgcaggt gatcctctat aaaagaggcg atgaagtagt aactgtgatc
    20281 atatcccggc tgaatacgca gcgtcatcgg ccacgctttc tggcgcgcgg cttctgccaa
    20341 taccgcaggt tgcaactggt cggcaagaaa ctgatcatta tcgccctgat cgataagcgt
    20401 cgggatcgca tcctgcgcgt tactggcata catcagcgcg caactatccc attccagcat
    20461 gcatttttgt cctcacctaa ataggtgcta aacgctttga ttccccacgg gacgctgcac
    20521 ggattcacaa tgggcgcaaa ggccgaaacg ctggtgtatt tacccggatt tttcagcgcc
    20581 ataatcagcg caccgtgacc acccattgag tgaccgctaa tggcgcagcg gtcgctgaca
    20641 ttaaattgcg actgaaccag cgccggtaat tcatcgcgca gataatcata catccggtaa
    20701 tgcgtcgccc acggcggttg cgtggcatta agataaaagc ctgcgccctg gcctaaatcg
    20761 tagccatcgt cgttggcaac cttttcgccg cgcgggctgg tgtctggcat caccagtaca
    20821 atccccagtt ccgccgctac ccgctgggca cccgccttgg tggtgaagtt ctcgtcattg
    20881 caggttaatc cggaaagcca gtacagcact ggtggcgggg tgtgatcacg aggggggggg
    20941 agaaagatac tgaacgtcat cgggcagttt aaggtactgg agtcgtgtcg ccagcgttgc
    21001 tgccagcctt caaaacagcg gtgctcttcg agcatttcca tgcgtggctc cttgttgtgt
    21061 tgtttgcaat ttcttcatca taccgtttat tcaatgtgct gtgagtaact ttcacttccg
    21121 tatttgcata acgatgtttt aacatctgct gatgaaaggc agcggcaatt acaataatta
    21181 tcgctgtgaa tactggatta tgtgcgccgc ctcacgcaca ataatcaagc tgtaaatcag
    21241 cttaataact ttgcccccac gcagggtgga ggcgtcacac ctgcaggaga aatcataaat
    21301 gccatcactc agtaaagaag cggccctggt tcatgaagcg ttagttgcga gaggactgga
    21361 aacaccgctg cgcccgcccg tgcatgaaat ggataacgaa acgcgcaaaa gccttattgc
    21421 tggtcatatg accgaaatca tgcagctgct gaatctcgac ctggctgatg acagtttgat
    21481 ggaaacgccg catcgcatcg ctaaaatgta tgtcgatgaa attttctccg gtctggatta
    21541 cgccaatttc ccgaaaatca ccctcattga aaacaaaatg aaggtcgatg aaatggtcac
    21601 cgtgcgcgat atcactctga ccagtacctg tgaacaccat tttgttacca tcgatggcaa
    21661 agcgacggtg gcttatatcc cgaaagattc ggtgatcggt ctgtcaaaaa ttaaccgtat
    21721 cgtgcagttc tttgcccagc gtccgcaggt gcaggaacgt ctgacgcagc aaattcttat
    21781 tgcgctacaa acgctgctgg gcaccaataa cgtggctgtc tcgatcgacg cggtgcatta
    21841 ctgcgtgaag gcgcgtggca tccgcgatgc aaccagtgcc acgacaacga cctctcttgg
    21901 tggattgttc aaatccagtc agaatacgcg ccacgagttt ctgcgcgctg tgcgccatca
    21961 caactgatta aaaggcagga accatggagc gcaacgtcac gctcgatttt gttcgcggcg
    22021 tcgccattct ggggatcctg ctattaaaca tcagcgcctt tgggctacca aaggcggctt
    22081 atctcaatcc cgcctggtac gatgctatta cgccgcagga tgcatggacc tgggcatttc
    22141 tcgatctcat cggccaggtg aaattcctca cgctttttgc gctgctgttt ggtgctggcc
    22201 tgcaaatgtt gctgccccgt ggcagacgct ggatccagtc gcggttaacg ctgttagtct
    22261 tgttgggctt tattcacggt ttattgttct gggacggcga tattctgctg gcttacgggc
    22321 tggtggggct tggcgttttg ctgttattgg ggctgatttc cgacagccag accagccgcg
    22381 cctggacgcc ggatgcatcg gctattttgt atgaaaaata ctggaagctt cacggcggcg
    22441 ttgaagcgat cagtaatcgt gccgatggtg ttggcaacag tttactggca ctgggcgcac
    22501 agtatggctg gcaactggca gggatgatgc tcattggtgc ggcgctgatg cgcagcggct
    22561 ggctgaaagg gcagttcagc ttacgtcact atcgtcgtac tggttttgtg ctggtggcga
    22621 ttggggtgac cattaacctt cctgccatcg ccctgcaatg gcagctggac tgggcgtatc
    22681 gctggtgtgc cttcttactt caaatgccgc gggaactgag tgcgccgttt caggcgattg
    22741 gctatgcgtc gctgttttat ggcttctggc cgcaattgag ccgctttaag ctggtgcttg
    22801 cgatcgcctg cgtcggacgg atggcgctga ccaactatct attgcaaacg ctgatttgta
    22861 ccacgctttt ttaccacctc ggtttgttta tgcattttga ccgcctggag ctgctggcgt
    22921 ttgttattcc ggtatggctg gcgaatatcc tcttctctgt tatctggctg cgttacttcc
    22981 gccaggggcc ggtggaatgg ctctggcgtc agttaacttt gcgtgctgcc ggaccggcaa
    23041 tatctaaaac atcaagataa cgatctggat tacattcgtt aacaaaacgg ctgtaaccgt
    23101 ttccattgct gtgactcgat tcacgaagtc ctgtattcag tgctgacaaa atagccgcca
    23161 gcaagcagtc atttactgca atctcataac aggtagtgaa tatgatcacc attcgtgatg
    23221 tagcgcgtca ggctggcgtc tctgtggcaa cggtttcccg ggtgctcaat aacagcacgc
    23281 tggtcagtgc cgacacgcgt gaagcagtaa tgaaagccgt gagtgagctg gattatcggc
    23341 caaacgccaa tgcccaggcg ctggcaactc aggttagcga caccattggc gtggtggtga
    23401 tggacgtttc tgatgcgttt ttcggcgcgc tggtaaaagc ggtggatctg gtcgcccagc
    23461 agcatcagaa atacgtgcta atcggcaata gctatcatga agcggaaaaa gagcgtcacg
    23521 ccattgaggt gttaattcgc cagcgttgta atgcgttgat tgttcactca aaagcattga
    23581 gtgacgatga actggcgcaa tttatggata acattcccgg tatggtgtta atcaaccgcg
    23641 ttgtgccggg gtacgcccat cgttgcgttt gcctggataa tctcagcggt gcccgaatgg
    23701 cgacgcgcat gttgctgaat aacggtcatc aacgtattgg ttatctttct tccagccacg
    23761 gcattgaaga tgacgccatg cgtaaagcag gctggatgag tgcgttgaaa gagcaggata
    23821 ttattccgcc ggaaagctgg attggcactg gtacgccgga catgccgggc ggtgaggcgg
    23881 cgatggttga actgctgggg cgcaatctac aacttaccgc tgtatttgct tataacgaca
    23941 atatggccgc tggcgcactg acagcattaa aagataatgg cattgcgatt ccgttacatc
    24001 tctcaatcat cggtttcgat gatattccca tcgcccgtta caccgacccg caattaacga
    24061 ccgtgcgtta tcccattgct tcaatggcta aattagccac cgaactggcc ttgcaggggg
    24121 cagcaggcaa tattgatcct cgtgccagcc actgttttat gccgacgtta gtgcgtcgcc
    24181 attctgtcgc aacgcgccag aatgcggcgg cgatcactaa ctcaacaaat caggcgatgt
    24241 aaccgctttc aatctgtgag tgatttcaca gtatcttaac aatgtgatag ctatgattgc
    24301 accgttttaa tgttgtaacc cgtatgtaac agtgaataat cactttcacc gaggtaatag
    24361 cgtcataaca acaattaaag ccgttttctg gagcgttacc gggcatggaa gaacgaattt
    24421 taaaaagtga gcttcggcgt tcagtaacac ttcattaact ctactgcccc gccgagcatt
    24481 tatctcaagc actaccctgc ataagaaaaa ccggagatac catgaataag aaggtgttaa
    24541 ccctgtctgc tgtgatggcc agcatgttat tcggtgccgc tgcacacgct gctgatactc
    24601 gcattggtgt aacaatctat aagtacgacg ataactttat gtctgtagtg cgcaaggcta
    24661 ttgagcaaga tgcgaaagcc gcgccagatg ttcagctgct gatgaatgat tctcagaatg
    24721 accagtccaa gcagaacgat cagatcgacg tattgctggc gaaaggggtg aaggcactgg
    24781 caatcaacct ggttgacccg gcagctgcgg gtacggtgat tgagaaagcg cgtgggcaaa
    24841 acgtgccggt ggttttcttc aacaaagaac cgtctcgtaa ggcgctggat agctacgaca
    24901 aagcctacta cgttggcact gactccaaag agtccggcat tattcagggc gatttgattg
    24961 ctaaacactg ggcggcgaat cagggttggg atctgaacaa agacggtcag attcagttcg
    25021 tactgctgaa aggtgaaccg ggccatccgg atgcagaagc acgtaccact tacgtgatta
    25081 aagaattgaa cgataaaggc atcaaaactg aacagttaca gttagatacc gcaatgtggg
    25141 acaccgctca ggcgaaagat aagatggacg cctggctgtc tggcccgaac gccaacaaaa
    25201 tcgaagtggt tatcgccaac aacgatgcga tggcaatggg cgcggtagaa gcactgaaag
    25261 cacacaacaa gtccagcatt ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc
    25321 tggtgaaatc cggtgcactg gcgggcacag tactgaacga tgctaacaac caggcgaaag
    25381 cgacctttga tctggcgaaa aacctggccg atggcaaagg tgcggctgat ggcaccaact
    25441 ggaaaatcga caacaaagtg gtccgcgtac cttatgttgg cgtagataaa gacaacttgg
    25501 ctgaattcag caagaaataa gactgattgg gcgcagtcgt ttactgcgcc cttattgtat
    25561 ggccaacaag gtaaaattat atggtcagct caacgactcc gtcttccggg gaatacttgt
    25621 tggaaatgag cggtatcaac aagtcctttc ctggtgttaa ggcacttgat aacgttaatt
    25681 taaaagtccg gccacattct atccatgcat taatggggga aaacggtgca ggaaaatcga
    25741 cattattaaa atgcctgttt ggtatttatc aaaaagactc cggcaccatt ttattccagg
    25801 gtaaagagat cgatttccat tccgccaaag aggcactgga aaatggtatt tcgatggtac
    25861 accaggagtt aaacctggta ttacaacgtt cggtgatgga taacatgtgg ctggggcgat
    25921 atcccaccaa aggcatgttt gtcgatcagg acaaaatgta ccgcgaaacc aaagcgattt
    25981 ttgatgaact ggatattgat atcgatccgc gtgcgcgcgt cggcacatta tccgtttcgc
    26041 aaatgcagat gatcgaaatc gccaaagcgt tttcctataa cgcgaaaatt gtgattatgg
    26101 atgaaccgac ttcttcgtta accgaaaaag aggtcaatca tctgttcact attattcgta
    26161 aattaaaaga gcgcggctgc ggtattgttt atatctcgca taaaatggaa gaaatcttcc
    26221 agttatgtga tgaagttacc gtattgcgcg acggtcagtg gatcgccacc gaaccgctgg
    26281 caggactgac gatggacaag atcatcgcca tgatggttgg gcgttctctt aatcagcgtt
    26341 ttcctgacaa agaaaacaag ccgggcgaag tcatcctcga ggtgcgtaac ctaacgtcac
    26401 tgcgccagcc gtcgattcgc gatgtctcgt ttgatctgca taaaggggag atcctcggta
    26461 ttgccgggct ggtgggggcg aaacgtaccg atattgttga gacgttattt ggtattcgcg
    26521 agaaatcggc tggcaccatt acgttgcacg gcaaacagat caataaccat aatgccaacg
    26581 aagccataaa ccacggattt gcactggtaa ctgaggagcg ccgctcaacg ggaatttatg
    26641 cctatctgga tattggtttt aactcgttaa tttccaatat tcgcaactac aaaaataaag
    26701 ttggtttact ggataactcg cggatgaaaa gcgataccca gtgggtgatt gattcgatgc
    26761 gggtaaaaac gccgggtcat cggacgcaaa ttggttcgct ctccggtggt aatcagcaaa
    26821 aggtgattat tggtcgctgg ctactaacgc aaccagaaat attaatgctc gatgaaccga
    26881 cgcgcggtat tgatgtcggg gcgaagtttg aaatttatca gttaattgcc gaactggcga
    26941 agaaaggcaa ggggattatt attatctcct ctgaaatgcc tgagttgtta gggataacag
    27001 accgtattct ggtcatgagc aatggtctcg tttccggaat tgtcgataca aaaacaacaa
    27061 cgcaaaacga aattctgcgt cttgcgtctt tgcaccttta agattagggg cttcccatga
    27121 gtgcgttaaa taagaaaagt tttcttactt acctgaaaga gggcggtatt tacgtcgttc
    27181 ttttagtttt gctggcgatt attattttcc aggacccaac atttttaagt ctgttgaact
    27241 taagtaatat tctcacccag tcatcggtgc gtattattat cgcgctcggt gtggcagggt
    27301 taattgtcac ccaggggacc gatctttctg ctggtcgtca ggtagggctg gcggcagtgg
    27361 tggctgcgac attattgcag tccatggata acgccaacaa agtgttcccg gaaatggcga
    27421 cgatgccgat tgcgctggtt attctgattg tctgtgccat tggtgcggtg atcggtttga
    27481 tcaacggtct gattatcgct tatctcaacg tgacgccgtt cattaccacg ctcggcacga
    27541 tgatcatcgt ctatggcatc aactcgctct attacgactt tgtcggggcg tcgccaattt
    27601 ctggttttga cagtggcttc tctacctttg ctcagggctt tgtcgcgctg gggagtttcc
    27661 gtctctctta catcaccttc tacgcgttga ttgcggtggc gttcgtctgg gtgttgtgga
    27721 acaaaacccg cttcggtaag aacatttttg ccattggcgg taacccggaa gcggcaaaaa
    27781 tatctggtgt caacgtcggc ctgaacctgc tgatgatcta cgcgttgtct ggcgtgttct
    27841 atgcctttgg cgggatgtta gaagccggac gtatcggctc tgccaccaac aacctcggct
    27901 ttatgtatga gctggatgct atcgcggcgt gcgtggtagg cggtgtatcg ttcagcggcg
    27961 gtgtggggac ggtgattggc gtggtgaccg gggtaattat ttttaccgtc atcaactatg
    28021 gcctgacgta tatcggcgta aacccatact ggcagtacat catcaaaggg gcgattatta
    28081 tcttcgccgt agcgctggat tcactgaaat acgcgcgtaa gaagtgatgt gattgcggat
    28141 attgccggat gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg cctttcttaa
    28201 acttcacttc cccgagctca atacaaccca ccgggcagac gtgaccacaa agcagacaac
    28261 ccacacattt ctcggtatta caatgcggtg tgcgggtttt ctcgctccat tccatcgcct
    28321 ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca gccaacacat ttgtcaagat
    28381 tgatacgggg atagacaata taactgcggt ctaaatcttc cgccgggaca atattgttat
    28441 tcgccagacc taccatctcc tgcaacgaat caaatccctg atcggcgaga taatgcgata
    28501 acccgctcgc catatcttct acgatgcgat agccgtactg cataatgccg gtggtgacct
    28561 gtaacgttgc tgcgccgagc aataaaaact ccgccgcatc ttcccaggtt tcaatgccgc
    28621 cgataccgct gattgggaaa tcgcgcagtt ctggatgggt gcgcatttgc tgaatgaagc
    28681 gcagggcgat cggttttacc gctttaccgg aatatccgga aatactcgat ttaccgttaa
    28741 cgatcggcat accgattttc tgattaagat cgatattggt gatggattta acggtgttaa
    28801 ttgccgcaat gccatctgcg ccgccgcgct tcgccgccag cgccacttcg cacatatcac
    28861 cgatattcgg cgtcatcttc gccagcattg gcagcgtgga accccgtttc actgcccgac
    28921 aatatttttc taccagctcc gggctttgcc cgacatcgct acccatcgca tgagaagtca
    28981 tttgcggaca ggagaagtta cactcgatca tatccgcgcc agctgctccc attgctgctc
    29041 attttccccc atgatcgaag cgatcaatac tttgtccggg taatcttcct tcagccgacg
    29101 cagggcggtc agattctctt ccaacggatg ttcagcaatc tgctccatat ttttgaagcc
    29161 gataaaaccg gtatcttctt tcaccagatg atcaaaacgc ggcgagactt cgttggcgat
    29221 aaaaaagccg atcgttttaa acaccacacc gccccaacct gtgtcgtagg ctttggcaca
    29281 catctcatag cagttgccta ccggcgaaga agagaggcag aacgggttgg gaaagttcac
    29341 gccgcaaaaa gtaatcgaaa gatctttcgt taacatgagc aagctccctc taaatagtga
    29401 tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt tttgtcaccc
    29461 tcaacaatat cgccagcagc aaagacttgc ggatcgcgga tctgataatt ttgtgttttg
    29521 atggtgttac gctgctgctc taactccgca aaggcatcca gtctggcatg ctgaccgacg
    29581 gcgagaataa ttttatctgc cgccatcgtc agttcgcctg ataaccgtac atgcttaaac
    29641 gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga aacgcccagc
    29701 tcccgggcgc tggcaaactc tttttcgctt gccggaaact catctaactc ttcacgtgct
    29761 acgcaagtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc catcgcgaca
    29821 tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt
    29881 acacgttgca ggaagtcgac ggcaatctca acgtcgctat gctcaaacag cggtagcccg
    29941 gaaccgcttg ataaccccac ggtgaccagt accgcgcggt tttccgcttt cagttgctcc
    30001 agggtgagtg ttttaccgat ttcgttattg cacttaatgg tcacgcccat tttttcaata
    30061 cgggcgatct ctgcatccag cactgattgc ggtaaccgga attgcggaat accgttacgc
    30121 aaccagccac cggggtgcgc ttctttctca taaatcgtga cgtcataacc ctggtttgtc
    30181 agtgtcacac tggcctgcaa tccggcagga cctgcgccaa taatcgcgac tttgccgagc
    30241 gttttagtac cgggctgata aatttccatc ccggtttgtt gttcaaaatc agtaacaaaa
    30301 cgctgtaagc ggccgatatc aatgggagca tcaacaccgg cacgggtaca accgctttgg
    30361 cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt attttcgcga
    30421 attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa tttccccgga
    30481 tcggtctgtg ccgggcaagc ctgactacag ggagcgtcgt gacataataa acagcgagag
    30541 gcttctttaa tcgccagtaa cggcgtaaaa gccggagtga gttcatccag ataattttgt
    30601 tgcggcatgt gatcttcctt aatgctatga gtaattatgg cgaacgctaa ataaatattg
    30661 cgtgctgata ttaaggctat gcgcgtgggg aaatttaagc tgtgaaagga ttcacaaata
    30721 gctgcggttc agaatgttat atccgcagct tattgagtga agtaataatt attctcaata
    30781 attaacagaa aagagaaaat attaacgata gaaaataaga taaacaatca tgccgataga
    30841 taaaattccg aagccaatat atgccaccat ataagcgaag aagagtccgg gattttttgc
    30901 tcgccagaca tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg
    30961 ccagcctttc catgcttcgc ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac
    31021 aaaaaactgc tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt
    31081 tcgggtgtaa cggcgggata cccctgcgca tcttccggca cctggtgcat agccggaata
    31141 gggaccagcg gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc agccagcgca
    31201 ccgaaacggg cggcaaattc gcgaatacgt actgacagca tatctttcgg tgacggtacg
    31261 gcataacact gagcctgaat ccccatatgc agcgcaataa ataatgctcg ctcacagtgg
    31321 aaacgttggg tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg
    31381 gaatccagcg tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca
    31441 gcgattaaat ctttgcgcat ggtcatcggc tcgttataac tttgcaatgc gttatcgccg
    31501 ctcagtaata gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg
    31561 tagcgataat actgattaat aacgccggta cgataatatt ttgctgtgcc gagcaccacg
    31621 ccgacctggc ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag
    31681 ctcatccagc gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg
    31741 ctgaggaaca cgcgctttaa catgcaggag actcgagcta aggaaaattt ccatcaggct
    31801 actttacccg ctggttttgc gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca
    31861 ggagagggct ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga
    31921 gaagcacgcg gtcgatactg tgacagccga gagctttcag cgttgcggag gtggcatccc
    31981 actgaatcaa cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat
    32041 cataaccctt gagattcaac agaattaacg ccccctgcaa cggcggcaga gtcgggttga
    32101 atgcggcgtt ttcagcgtag ctgccgctga aaatacgacc gtctttacat tccagcgcga
    32161 caccgcttgg tgacttactg taaggcatgt gcgaacggtt tgccgctgca atcgccgcct
    32221 gagaaagcgc atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc agcagcgttt
    32281 taatctccag atctttcggc ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct
    32341 cgcggcccgg caaatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac
    32401 ggcagtgacc acaaggcgta tagttaacgg tgatggctgc aagcgctttt tcaccactca
    32461 accaagcgtg gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gttgcaccaa
    32521 taaattccat attggcacca aaataccagg ttccgctcac accgcgcgca attgcgccaa
    32581 cattaaaatt cgacaatggc gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg
    32641 ccagcgcgtc ttcgtccagt cccgttgcgc tcttcagcga tgagacttgc tccccggtca
    32701 acaaagcggg gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg
    32761 caagttgggc aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa
    32821 tgggataaca gtttacgaac ccgttatggc tttatatgtg atctgaatct cattaattat
    32881 gcaacacgtg aaacttacat caaaaatgcg cgacgtatcg caaattttag cccattactg
    32941 ccagaataat cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca
    33001 gcgctagcga actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga
    33061 gggcgtgcga ggcagtcccc attgccagac cgcgcgcagc tttggtacga atacgcatcg
    33121 cattaagcaa tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg
    33181 cgctgattgc cggaataccg ccaatactgc cgccaaccgc cattgcaata ggcgtggtga
    33241 ctgatttcgg caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg
    33301 aagtccccgt caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc
    33361 agcgcgcgcg gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg
    33421 gttgcagcag atcgtttagc acttcgctac ctttaaagta actgtcgtaa gagatgccgg
    33481 tcagcattaa aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa
    33541 acttataccg tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg
    33601 accaccagat attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc
    33661 gtgtaccagt tgtgaactcc agctcaccac cagaaaaacc accagcgtac tgacagcaca
    33721 ggaaaccact accgggccaa actgcgcgcg gagcaaatca aaatattgca tgacgcctac
    33781 gccaatcggc acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac
    33841 ccatttcgcc ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat
    33901 gctgcccgga atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc
    33961 ataaatcagg acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat
    34021 caacacccca gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca
    34081 cattaagaaa tcagcgctta ttttttcgct acccggtatt acgcaatgag cagcgaactc
    34141 aggcatacat caagcataga gagggattgc agtttgcagc aatccctctt tgcagacctt
    34201 taatccgctg accataggcg ctaacatagc gcctcatttt tttgcgggtg atgatatgcg
    34261 tgtgttactg gcaccgatgg agggagtgct tgactctctg gtgcgtgaat tgctgaccga
    34321 agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc
    34381 ggtaaaagtc tttcatcgca tttgccctga gctacaaaac gccagccgga caccatctgg
    34441 tacgctggtg cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc
    34501 ccgtgcggtg gagttaggtt tctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac
    34561 ggttaacggt agcggcggcg gggcgacgtt actcaaagat cctgaactca tctaccaggg
    34621 ggcaaaagcg atgcgtgaag ctgtaccggc gcatttgcct gtcagcgtga aagtgcgtct
    34681 gggctgggac agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggcgc
    34741 tacggagctg gtggtgcatg ggcggacgaa agagcagggt taccgcgcgg agcatattga
    34801 ctggcaggcg attggcgaga ttcgccagcg gctgaatatt ccggtgattg ccaacggtga
    34861 aatctgggac tggcagagcg cgcaacaatg catggcgatc agcggctgcg acgcagtgat
    34921 gattggtcgc ggggcgctca atattcccaa cctgagccgg gtggtaaaat ataacgaacc
    34981 gcgaatgccg tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca
    35041 gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa
    35101 agaatacgat gaagcaacgg aattatttca gcatgttcgg gtgttgaata actcccctga
    35161 tattgcaagg gctattcagg caattgatat cgagaaactc taaaaaccat agtgatgttt
    35221 ttgacaatgt gttagctctt tatcaatagg ccgcacggtt gtttggtggc gtcaaaatcg
    35281 ccgaggcgtt ccctgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtttta
    35341 cagggatgtt acctcgcgcc cgacccggta gccgtaaggg ataagccgag ggcaccgcgc
    35401 agcggcgatt ttgttcgcca gagcccgggg gggcaggggg cggcggcgat tggtcgcccc
    35461 ctgcgcgctc cttgcgccag tgattatatg aagcaatgca catgacagga gcgcaacagg
    35521 atgagagact gaaatattct cgttattttc ggatctgatg acattaatta attttaaaat
    35581 atcatcttaa atacgccagt caccaccagt agcccaatca ggaaaataat caatacagcc
    35641 cagagtataa ttttcattgg cgttccttta tttgtgagtg ctgaataagt ataggaaaaa
    35701 acgaagccct ttgccgttat ccataaacga ttaagataaa tcggaaaata tcatttactt
    35761 agtctatatt tttctgggta tgtcgtttta ttgctactga tgtattgcca cgcgaaacta
    35821 aaaaatatat cgcaacacac ggtaatctcc gcgcaccgtt tcttacctga ttattccccc
    35881 atgaatcgtg attcctttta tccagccatc gcctgttttc cgctgttact gatgctggcc
    35941 gggtgtgcgc ctatgcatga aacccgccag gcgttaagcc agcaaacgcc cgctgcacaa
    36001 gttgacaccg cattacccac ggcgctgaaa aatggttggc cagacagcca atggtggctg
    36061 gagtatcacg ataatcagct cacttcatta attaacaatg cactacagaa cgcaccggat
    36121 atgcaggtgg ctgaacagcg catttagcta gctgaagccc aggcgaaggc agttgccact
    36181 caggatggtc cgcagatcga cttttcggcg gatatggagc ggcaaaaaat gtcggcagaa
    36241 gggctaatgg ggccgtttgc tctgaacgat ccggccgcag gtacgaccgg tccgtggtac
    36301 accaacggaa cttttggctt aacggcgggc tggcatctcg atatctgggg aaagaatcgg
    36361 gcggaggtta ctgcccgcct gggtacggtt aaagcacggg cggcggaacg cgagcaaacc
    36421 cgccaattgc tggctggcag cgtagcccgc ctgtactggg agtggcaaac ccaggcggcg
    36481 ttaaacacgg tcttgcagca aatagaaaaa gagcagaaca ccattatcgc gaccgatcgc
    36541 cagctatatc agaacgggat tacttcttca gttgacggtg tggaaaccga tattaatgcc
    36601 agcaaaaccc ggcagcagct caacgatgtc gcggggaaaa tgaaaattat tgaggcacgg
    36661 ttaaatgcac tcacaaatca ccagactaag tcattgaagc ttaaaccggt cgcgttgccg
    36721 aaagtggcaa gccagcttcc tgatgaactg gggtactcct tactggcccg gcgggcagat
    36781 ttgcaggcgg cgcactggta cgttgagtca tcgttaagca ctattgatgc ggcaaaagcg
    36841 gcattttatc ctgacatcaa cctgatggcc ttcctgcaac aggatgcgtt gcacttaagc
    36901 gatctgttcc gtcattccgc gcagcaaatg ggcgttacgg caggcctgac gctacctatt
    36961 ttcgatagtg gtcgtcttaa cgccaatctc gatatcgcaa aagccgaaag caacttgtct
    37021 atcgccagct acaacaaagc ggtggttgaa gcagtgaatg acgtggcgcg ggcagccagt
    37081 caggttcaga cactggcgga gaaaaaccag catcaggcgc aaattgagcg cgatgccttg
    37141 cgtgtggtag gtcttgcgca ggcccgcttt aacgcgggca tcattgctgg ttcccgcgtc
    37201 agcgaagcca gaatcccggc gctgcgtgag cgggccaatg gcctgttatt gcaagggcag
    37261 tggctggatg cctccattcg actcaccggt gcgctgggcg ggggttacaa acgctgatga
    37321 tatgcgcctt ctatacttaa cgtttattca gcgttaagtg gagaactcga tggcataggt
    37381 cgcgataatt accgcttccg attcggggat cggcaaagag tgcgcgttat tactggcgca
    37441 gcaggggttt gatattggca ttacctggca ctcagatgaa gaaggggcaa aagataccgc
    37501 gcgtgaggtg gttacccacg gcgtacgtgc ggagatcgtg caactggatc tcggcaatct
    37561 gccagaaggt gcgcaggcgc tggagacact catccagcga tttgggcgta ttgatgtgct
    37621 ggtgaataat gcgggtgcaa tgaccaaagc gccgtttctt gatatggctt ttgatgagtg
    37681 gcgcaagatt tttaccgttg atgttgatgg tgcattttta tgctcgcaaa ttgcggctcg
    37741 tcagatggtg aaataagggc agggcggtcg catcatcaac attacgtcgg tacatgaaca
    37801 tacgccgctg ccggatgcca gcgcctacac agccgctaaa catgcgctcg gcgggttaac
    37861 caaaacgatg gcgctggagt tggtcaggca taagattttg gtgaacgcag tcgcgcctgg
    37921 ggcgatagcc acgccaatga acagcatgga tgatagcgac gtgaagcccg acgcggagcc
    37981 ttcgattcct ctacggcgtt ttggcgcaac gcatgagatt gccagcctgg tggtgtggct
    38041 ttgttcggag ggcgcaaatt acacgacagg gcagtcgttg atagtggatg gcggctttat
    38101 gttggcgaat ccgcagttca acccagaata gcgcagtgag gtgcgtggat atttcaatgg
    38161 gttaggccag ataagacacc agcgtcgcat caggcatgga acacccggtg ccggatgtgg
    38221 agtgaatgct tatctgtggc aactgcttta cgcctgatta tccggctttt tcttcccgcg
    38281 ccgtttcagc caccagcgca cgcccaccac cagaaccacg accagaatca accagaccca
    38341 gtgcttcaaa tgctggtcga gattgtgcaa ccacggcgca atcacctgac caccagcgta
    38401 accaatagtg gtaaaaatca acgcccaggc aaatgcgccg agaatattca gcggcagaaa
    38461 gattttcggc ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc gaaagccata
    38521 cataaagcgc gtaccaatga caaacagata cggatggcgt tggataagtt tctgtgcccg
    38581 ctcaatttta tcctgatgtt tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc
    38641 gcacagatag agcacctggt cgccaatcat gccaccaagc gccacagaaa gtaccaccag
    38701 tgggaacttt aatagcccct gatgtgccgc aacgcctccc agcaaagtca cggtttcacc
    38761 ttccgccagg ctaccgataa ccagcgcggc ataaccatat tgtgagatga gtgtattgag
    38821 atccataggc cagtttgtct ccctttggtt gcctgtaagc atacaccctg tgaatcattc
    38881 acgctgaaaa aggacataca tcagacggtg caaattacat ggatgaacaa aaaataatca
    38941 caagctggct tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag
    39001 ccatgtctgg ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa
    39061 cgaaacgatt tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat
    39121 ttgcgccttc attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa
    39181 gttatcctgg tttaccggac tggcgctggc tgtcttgttt tacggcgtta tgctggctgg
    39241 ggttgcggtg atggggcggg tcatctggtg gatggcgcgc aattacccgc agcgtccgtc
    39301 tcttgcgcac tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct
    39361 ggtggcgctt tatccactgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta
    39421 caccggctac ctgctttacc ttggtattcc gagcttcctg aatatcaaca aagaggaagg
    39481 ccttagcttc tccagttcga cgctggcgat tggcgtgctg gtactggaag tgctgctcac
    39541 cctaaccgtt atactatggg gctacggtta tcgtttgttc tagaaagttc tttgacgttg
    39601 cattgctggc gtaaatgtca gcaatgcagc atttactcac gattctcctt tgacgatcgc
    39661 actttttgct cgttatgatg gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc
    39721 aacccgtgcg cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta
    39781 tttagcttgg ccctgatgct ggctgtgcct tttgcaccgc aggccgttgc gaaaacggca
    39841 gccgctacca ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg
    39901 aataccaaca aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc
    39961 agcaaattaa tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta
    40021 aaagtggata tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaat
    40081 agcgaaatca gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg
    40141 gcggcaagcc ttgcgcacca ttatcccggt ggttacaaag cctttattaa ggcaatgaat
    40201 gcgaaagcga aatcgctcgg aatgaacaac acgcgctttg ttgaacctac cggattgtcg
    40261 gtgcataacg tttcaactgc ccgtgactta accaaactgc tcattgccag caaacaatat
    40321 ccgttgatcg ggcagttaag tactacccgg gaagatatgg caaccttctc taacccgacg
    40381 tatacgctgc cgttccgcaa tactaatcat ctggtgtatc gcgataactg gaatattcag
    40441 ttaaccaaaa ccggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc
    40501 aataataaac cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc
    40561 gatgccagcc gcctgcgtac ctggattgaa accggtaaag tgatgcctgt gccggcagca
    40621 gcgttgagct ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag
    40681 aacgattaag tgaattcgga tggcgatact ctgccatccg taatttttac tccacttcct
    40741 gccagttttt ccgtttactg gttttaccga tccccggatt catgctattg gtcggatcat
    40801 tctcgcgata gaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgct
    40861 cggcagggta ctgcgcgccg cgctgctgta gcagttccag catctgttct tttaacgcat
    40921 gcacatccac gccttttttc actatgtaat cctgatggaa gacatagcac ataaaatggc
    40981 cgtaatagag cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact
    41041 cggtgtcgtt acgccgcaga gcgatatcca gtgccagaat gtcttcgact tcatcggaat
    41101 gcaccgcctg ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc
    41161 taccttcctc cggcgtacag acaaagaaat cgccctcggc ctgtttgaaa taatccacca
    41221 gccacgattt ggcttcaccg acgccatcgc ctgccatttt taacagcaga tgatgctcgt
    41281 atttatcgcg ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt
    41341 tttgcattgc acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat
    41401 cggtgcgtcc cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca
    41461 tcaggaaggt gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt
    41521 ccccggcaac cggcagattt tcgaagttag ccagaatatg acggcggatt tcggtcagca
    41581 cttccggctg gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat
    41641 caagacgtac cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat
    41701 ccggatcggc gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat
    41761 agtcataatc gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcgt
    41821 catccagctt gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca
    41881 ccagcgtcag tttgccatct tcatctatac gcgcgaataa cgacatttcg gtatacgccg
    41941 ggccacgttg caccagcgag ccgccggagt tgttacaaat accgccgatg accgatgcgc
    42001 ctatacacga tgatccaatc accgaatgcg gttcgcgtcc cagcggtttg agggcttttt
    42061 ccagcgaata gagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccgagaacgt
    42121 gcagcttgtc gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt
    42181 ttggcgtcga tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg
    42241 cggtgacgca ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag
    42301 ccagcgcgtc gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat
    42361 cggtgagcag gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat
    42421 cagttgttgt catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact
    42481 agaatagcgc cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac
    42541 tgagcattct ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt
    42601 cgctgccacg tcgggcaaca aaggaagaaa aatccatatg aaatggctat gttcagtagg
    42661 aatcgcggtg agtctggtcc tgcagccagc actggcggat gatttattcg gtaaccatcc
    42721 attaacgcct gaagcgcggg atgcgttcgt caccgatctg ctcaagaaaa tgacggttga
    42781 tgagaaaatt ggtcagctgc gcttaatcag cgtaggcccg gataatccga aagaggcgat
    42841 ccgcgagatg atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga
    42901 tatccgcgcc atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt
    42961 tgcttacgac gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc
    43021 gtcttttaac ctcgatgcgg tgaaaacggt cgggcgtgtc tctgcttatg aagcggcaga
    43081 tgatggcctg aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg
    43141 acgtgcttcc gaaggttttg gcgaagatac gtatctcacc tcaataatgg gtaaaaccat
    43201 ggtggaagcg atgcagggta aaagcccggc agatcgttac tcggtgatga ccagcgtcaa
    43261 acactttgcc gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag
    43321 tccgcagcgc ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag
    43381 cggcgcggtg atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcctg
    43441 gctgctgaaa gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca
    43501 cggtgcaatc aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg
    43561 cgtggcgctg aaatccggaa tcaacatgag tatgagcgac gagtactact cgaagtatct
    43621 gcctgggttg attaaatccg gcaaagtgac catggcagag ctggacgatg ctgcccgcca
    43681 tgtactgaac gttaaatatg atatggggtt gtttaacgac ccatacagcc atttggggcc
    43741 gaaagagtct gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg
    43801 tgaagtggcg cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa
    43861 aaaatcggcc accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg
    43921 cagctggtcc gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggattaaaaa
    43981 ttccgtcggt gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa
    44041 aggcattatc gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc
    44101 gcaagagatg attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt
    44161 agtcggtgaa gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc
    44221 gcaaggccaa cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt
    44281 gctgatgaac gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct
    44341 ggaaacctgg tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga
    44401 ttacaacccg tccggcaagc tgccaatttc cttcccgcgt tctgtcgggc agatcccggt
    44461 gtactacagc catctgaaca ccggtcgtcc gtataatgcc gacaagccga acaaatatac
    44521 ttcgcgttat tttgatgaag ctaacggagc gctttatccg ttcggctatg gtctgagcta
    44581 taccactttc accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa
    44641 agtgacggcc agcgtgcagg tgacgaacac cggtaagcgc gagggggcca cggtagtgca
    44701 gatgtacttg caggatgtga cggcttccat gagtcgccct gtgaaacagc tgaaaggctt
    44761 tgagaaaatc accctgaaac cgggcgaaac tcagactgtc agcttcccga tcgatattga
    44821 ggcgctgaag ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt
    44881 atttatcggc actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt
    44941 tagtaagggc gcgacgtttg cgccctttgc ctgatgttca tatcttaact tgctgaattt
    45001 tcgccctttg taggccggat aaggcgctcg cgccgcatcc ggcaggaaac gccggaccga
    45061 atatgcctct taagtattta tctgattctg caactgaatc cttccgctca ggctaactct
    45121 gtcattatca actatgcttt tctcttaatt cgctgaaaac aacagtaaaa gaatgaggaa
    45181 agcatcatgc cactcttaaa gctctgggca ggttcactgg ttatgttggc agccgtgagc
    45241 ctgccgctac aggcggcttc ccccgttaaa gtcggttcaa aaatcgatac cgaaggcgcg
    45301 ctgctcggca acatcatctt gcaggtactc gaaagccacg gcgtaccgac ggtgaataaa
    45361 gtgcaactcg gaacgactcc tgtggggcgc ggggcgatta cttccgatga actggatatc
    45421 tatccggaat ataccggcaa tggcgctttc ttctttaaag atgaaaacga tgcagcgtgg
    45481 aaaaatgccg ggcagggcta cgagaaagtc aaaaagctcg atgcggagca caacaagtta
    45541 atctggctga cgcccgcgcc tgcaaataac acctggacta tcgccgtgcg tcaggatgtg
    45601 gcagagaaaa acaaactcac ttcgcttgcc gacctgagtc gttatctgca agagggcggc
    45661 accttcaaac tggcagcctc ggcagagttt atcgaacgcg ccgatgcgtt acccacgttt
    45721 gaaaaagcct atggctttaa gctcggtcag gatcagttgc tgtcactggc tggtggcgac
    45781 acggcggtga cgattaaagc cgctgcccag caaacctctg gcgttaatgc cgcaatggct
    45841 tacggcactg acggtccggt cgcggcgctg gggctgcaaa ccttaagcga tccgcaaggc
    45901 gtgcaaccta tctacgcgcc tgcaccagtg gtgcgtgaat cggtgttgaa ggagtatccg
    45961 caaatggcac agtggctaca gccagtcttc gccagcctcg atgcaaaaac attgcagcaa
    46021 ctgaatgcca gcattgcagt ggaaggactg gatgccaaaa aagtggctgc cgactacctg
    46081 aaacaaaaag ggtgggcgaa gtaatttccc gtgacttatc tacgtattaa tcctgttctg
    46141 gcgctgctgt tgttgctgac ggcaatcgca gcggcgctgc cgtttatcag ttacgcgcct
    46201 aatcgtttag tctcgggtga agggcttcat ctctggcaac tgtggccgca aacgatctgg
    46261 atgctggtgg gcgttggttg cgcctggctg acagcctgtt ttgtccccgc taaaaaaggc
    46321 agcatttttg cactcattct ggcgcaattc gtcttcgtat tgttggtgtg gggagctgga
    46381 aaggcggcga cccaactggc gcaaaatggc agtgcgctgg cgcgtaccag cctcggcaga
    46441 ggtttctggc tggctgcggc gctggcattg ctggcctgta gcgatgccat ccgccgaatc
    46501 tccacgcatc cgctgtggcg ctggttgttg catatgcaga ttgccattat tccgctgtgg
    46561 ttgctgtact ccggcacgct taacgatctc tcactaatga aagaatacgc caaccgtcag
    46621 gatgtgtttg acgacgcgct ggcacaacat ctgacgttgc tgtttggtgc ggtgctgcct
    46681 gcgttagtga ttggtgtgcc gttgggcatc tggtgctact tttccactgc gcggcagggg
    46741 gcaatttttt ctctgctcaa tgtcattcag accgtgcctt cggtggcgct ctttggcctg
    46801 ttgattgcgc cgcttgccgc gctggttacg gcctttccgt ggctggggaa gctcggcata
    46861 gcagggaccg gaatgacacc cgcactgatt gcgctggtgc tctatgcctt gctgccgctg
    46921 gtgcgaggcg tggtagtcgg tttgaaccag atcccgcgcg atgtgctgga gagcgccaga
    46981 gcgatgggga tgagcggggc gcagcgtttc ctgcatgttc agttaccgct ggcgttaccg
    47041 gtatttctgc gcagcctgcg ggtggtgatg gtgcaaactg taggtatggc ggtgattgcg
    47101 gcgttaatcg gcgcaggcgg ttttggtgcg ctggttttcc aggggctgct aagcagcgcc
    47161 attgatttag tgttgctggg ggtgatcccg gtaattgttc tggcggtgct taccgacgcg
    47221 ctgttcgatt tgcttatcgc actgctgaag gtgaaacgta atgattgaat ttagccatgt
    47281 cagcaaactg ttcggcgcac aaaaagccgt taacgatctc aatctcaatt ttcaggaagg
    47341 gagtttttcg gtgctgattg gcacatctgg ctccggcaaa tccaccaccc tgaaaatgat
    47401 taaccgcctg gtggagcatg acagcggagt gatccgcttt gccggagaag aaattcgctc
    47461 gctgccagta ctggagttgc gccgccggat gggctatgcc attcaatcta ttggcctgtt
    47521 cccccactgg agcgtggcgc aaaacatcgc taccgtgccg caattacaaa aatggtcgcg
    47581 ggcgcggatt gacgatcgta tcgacgaatt aatggcgcta ctggggctgg agtcaaattt
    47641 gcgtgagcgt tatccgcatc agctttccgg tggtcagcag caacgtgtgg gagtggcgcg
    47701 tgcactggct gccgatccgc aagtcttact gatggatgaa ccttttggcg cactggaccc
    47761 ggtaacgcgc ggcgcgttgc aacaagagat gacgcgcatt caccgtttgc tggggcgcac
    47821 tattgtgctg gtcactcatg atattgatga ggcgctacgg ctggcagaac atctggtatt
    47881 gatggatcac ggtgaagtgg tgcagcaggg gaatccgctg acgatgctga ctcgtccggc
    47941 gaatgatttt gtccgccagt tttttggacg tagtgaactg ggggtgcacc tgctttcgtt
    48001 acgtagcgtg gcggattacg tgcgtcgcga agaacgggca gaaggtgagg cactggcaga
    48061 agagatgacg ctacgcgatg cgctctccct gtttgtcgcg cggggatgcg aggtgctgcc
    48121 ggtggtgaac acgcagggcc agccttgcgg cacgctgcat tttcaggatc tgctggtgga
    48181 ggcgtaagcg tatgaagatg ttgcgcgatc cgctgttctg gctcattgct ctttttgtgg
    48241 cgctgatttt ctggctgcct tacagccagc cgctgtttgc tgccttgttc ccacaactgc
    48301 cacgacccgt ttatcagcaa gaaagttttg cagctctggc actggctcat ttctggctgg
    48361 tgggaatttc gagtttgttt gcggtgatca ttggcacagg tgccggaatt gctgttactc
    48421 gcccgtgggg cgcggaattt cgcccactgg tggaaactat tgccgccgtt ggacagactt
    48481 ttccgcctgt cgcagtgctg gcgattgccg ttccggtgat cggctttggt ctgcaaccag
    48541 cgattatcgc cttgatcctt tacggtgtgc tgcccatcct gcaggtgaca cttgccgggc
    48601 tgggagcgat tgatcccagc gtgacagaag ttgcgaaagg tatgggaatg agtcgaggtc
    48661 agcgactgcg taaggtcgag ctaccgctgg cggctccggt gattctggcg ggcgtgcgaa
    48721 cttcggtgat tatcaacatt ggtacggcga cgatcgcctc aacggtaggg gccagtacgc
    48781 tgggtacgcc catcatcatc gggcttagcg gatttaatac cgcgtatgtg atccaggggg
    48841 cgttactggt ggcactggcg gcgatcatcg cagaccgcct gtttgaaagg ctggtgcagg
    48901 cgcttagcca gcacgcaaaa taaaggtata acctgcgagc atgacgccac caattccgcc
    48961 taacgccata aacaggaaca gggcgatgac cccaatttt
//
LOCUS       DAJVPT010000016        47703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVPT010000016 DAJVPT010000000
VERSION     DAJVPT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(112..729)
                     /gene="tdk"
                     /locus_tag="OQM47_000919"
     CDS             complement(112..729)
                     /gene="tdk"
                     /locus_tag="OQM47_000919"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1479172.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1334..1747
                     /gene="hns"
                     /locus_tag="OQM47_000920"
     CDS             1334..1747
                     /gene="hns"
                     /locus_tag="OQM47_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1479173.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1891..2799)
                     /gene="galU"
                     /locus_tag="OQM47_000921"
     CDS             complement(1891..2799)
                     /gene="galU"
                     /locus_tag="OQM47_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1479174.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3001..4014)
                     /gene="rssB"
                     /locus_tag="OQM47_000922"
     CDS             complement(3001..4014)
                     /gene="rssB"
                     /locus_tag="OQM47_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1479175.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4106..5011)
                     /gene="rssA"
                     /locus_tag="OQM47_000923"
     CDS             complement(4106..5011)
                     /gene="rssA"
                     /locus_tag="OQM47_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1479176.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5124..5582
                     /locus_tag="OQM47_000924"
     CDS             5124..5582
                     /locus_tag="OQM47_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1479177.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5632..6474
                     /gene="purU"
                     /locus_tag="OQM47_000925"
     CDS             5632..6474
                     /gene="purU"
                     /locus_tag="OQM47_000925"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1479178.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6634..6718
                     /locus_tag="OQM47_000926"
     tRNA            6634..6718
                     /locus_tag="OQM47_000926"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6668..6670,aa:Tyr,seq:gta)
     gene            6765..6896
                     /locus_tag="OQM47_000927"
     ncRNA           6765..6896
                     /ncRNA_class="other"
                     /locus_tag="OQM47_000927"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7080..7757)
                     /gene="narI"
                     /locus_tag="OQM47_000928"
     CDS             complement(7080..7757)
                     /gene="narI"
                     /locus_tag="OQM47_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1479179.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7757..8467)
                     /gene="narJ"
                     /locus_tag="OQM47_000929"
     CDS             complement(7757..8467)
                     /gene="narJ"
                     /locus_tag="OQM47_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1479180.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8464..10002)
                     /gene="narH"
                     /locus_tag="OQM47_000930"
     CDS             complement(8464..10002)
                     /gene="narH"
                     /locus_tag="OQM47_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1479181.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9999..13742)
                     /locus_tag="OQM47_000931"
     CDS             complement(9999..13742)
                     /locus_tag="OQM47_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1479182.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14135..15526)
                     /gene="narK"
                     /locus_tag="OQM47_000932"
     CDS             complement(14135..15526)
                     /gene="narK"
                     /locus_tag="OQM47_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1479183.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15865..17661
                     /gene="narX"
                     /locus_tag="OQM47_000933"
     CDS             15865..17661
                     /gene="narX"
                     /locus_tag="OQM47_000933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1479184.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17654..18304
                     /gene="narL"
                     /locus_tag="OQM47_000934"
     CDS             17654..18304
                     /gene="narL"
                     /locus_tag="OQM47_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1479185.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18305..19699)
                     /gene="ychO"
                     /locus_tag="OQM47_000935"
     CDS             complement(18305..19699)
                     /gene="ychO"
                     /locus_tag="OQM47_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1479186.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19884..20237
                     /gene="ychN"
                     /locus_tag="OQM47_000936"
     CDS             19884..20237
                     /gene="ychN"
                     /locus_tag="OQM47_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1479187.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20281..20976)
                     /gene="chaC"
                     /locus_tag="OQM47_000937"
     CDS             complement(20281..20976)
                     /gene="chaC"
                     /locus_tag="OQM47_000937"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1479188.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21134..21364)
                     /gene="chaB"
                     /locus_tag="OQM47_000938"
     CDS             complement(21134..21364)
                     /gene="chaB"
                     /locus_tag="OQM47_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1479189.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21634..22734
                     /gene="chaA"
                     /locus_tag="OQM47_000939"
     CDS             21634..22734
                     /gene="chaA"
                     /locus_tag="OQM47_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1479190.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23139..23246
                     /locus_tag="OQM47_000940"
     CDS             23139..23246
                     /locus_tag="OQM47_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1479191.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23395..24249)
                     /gene="kdsA"
                     /locus_tag="OQM47_000941"
     CDS             complement(23395..24249)
                     /gene="kdsA"
                     /locus_tag="OQM47_000941"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1479192.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24285..25094)
                     /locus_tag="OQM47_000942"
     CDS             complement(24285..25094)
                     /locus_tag="OQM47_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1479193.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25098..25490)
                     /locus_tag="OQM47_000943"
     CDS             complement(25098..25490)
                     /locus_tag="OQM47_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1479194.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25487..26320)
                     /gene="prmC"
                     /locus_tag="OQM47_000944"
     CDS             complement(25487..26320)
                     /gene="prmC"
                     /locus_tag="OQM47_000944"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1479195.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26320..27402)
                     /gene="prfA"
                     /locus_tag="OQM47_000945"
     CDS             complement(26320..27402)
                     /gene="prfA"
                     /locus_tag="OQM47_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1479196.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27444..28700)
                     /gene="hemA"
                     /locus_tag="OQM47_000946"
     CDS             complement(27444..28700)
                     /gene="hemA"
                     /locus_tag="OQM47_000946"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1479197.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28914..29537
                     /gene="lolB"
                     /locus_tag="OQM47_000947"
     CDS             28914..29537
                     /gene="lolB"
                     /locus_tag="OQM47_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1479198.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29537..30388
                     /gene="ispE"
                     /locus_tag="OQM47_000948"
     CDS             29537..30388
                     /gene="ispE"
                     /locus_tag="OQM47_000948"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1479199.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30539..31486
                     /gene="prs"
                     /locus_tag="OQM47_000949"
     CDS             30539..31486
                     /gene="prs"
                     /locus_tag="OQM47_000949"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1479200.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31611..33290
                     /gene="dauA"
                     /locus_tag="OQM47_000950"
     CDS             31611..33290
                     /gene="dauA"
                     /locus_tag="OQM47_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1479201.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33345..33623)
                     /gene="ychH"
                     /locus_tag="OQM47_000951"
     CDS             complement(33345..33623)
                     /gene="ychH"
                     /locus_tag="OQM47_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1479202.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33901..34485
                     /gene="pth"
                     /locus_tag="OQM47_000952"
     CDS             33901..34485
                     /gene="pth"
                     /locus_tag="OQM47_000952"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1479203.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34602..35693
                     /gene="ychF"
                     /locus_tag="OQM47_000953"
     CDS             34602..35693
                     /gene="ychF"
                     /locus_tag="OQM47_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1479204.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36537..39404
                     /locus_tag="OQM47_000954"
     CDS             36537..39404
                     /locus_tag="OQM47_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1479205.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39504..41423)
                     /gene="dhaR"
                     /locus_tag="OQM47_000955"
     CDS             complement(39504..41423)
                     /gene="dhaR"
                     /locus_tag="OQM47_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1479206.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41651..42721
                     /gene="dhaK"
                     /locus_tag="OQM47_000956"
     CDS             41651..42721
                     /gene="dhaK"
                     /locus_tag="OQM47_000956"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1479207.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42732..43364
                     /gene="dhaL"
                     /locus_tag="OQM47_000957"
     CDS             42732..43364
                     /gene="dhaL"
                     /locus_tag="OQM47_000957"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1479208.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43375..44793
                     /gene="dhaM"
                     /locus_tag="OQM47_000958"
     CDS             43375..44793
                     /gene="dhaM"
                     /locus_tag="OQM47_000958"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1479209.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45113..46810
                     /gene="treA"
                     /locus_tag="OQM47_000959"
     CDS             45113..46810
                     /gene="treA"
                     /locus_tag="OQM47_000959"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1479210.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYRDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(46889..47328)
                     /locus_tag="OQM47_000960"
                     /pseudo
     CDS             complement(46889..47328)
                     /locus_tag="OQM47_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11973 a        12279 c        11684 g        11767 t
ORIGIN      
        1 tatagcggcg ttaaaaaact gctgagaagg gtatatagcc cggaagaagt gcgtaaaacg
       61 aactgacagg ataaaagtgc cctgctcacc ctgtcagtaa agaaattctt attaatcgtg
      121 gcgatgcctt tcctgaatag ccgttaatga gccgacttgt aacgcctctt tatagtgttt
      181 acggcataca gaaacgtatc gttcattacc accaattacc acctgctcac cttcgttata
      241 aggtctgcct gcttgatcaa gacgcagcac catgcttgct ttacggccac aaaaacagat
      301 ggtttttaat tcaaccagtt tgtcggacca tgccagtaag tattggctgc caataaataa
      361 ttcacctcga aaatcggtac gtaaaccata acaaagtacg ggtatatcga gttgatcgac
      421 aacctccgat aattcatata cttgttgtct ggttaaaaac tggcattcat caaccagtac
      481 gcaatgaatt gcctgctgtt catgttccgc acgaatctca tcaaataatg atgaattttg
      541 gttaaataat tttgcaggcg atgacaaacc tatacgcgaa ctgactttcc cggcaccaaa
      601 gcgatcatca atttctgccg tatatacgac agtgcgcatg ccgcgttcct ggtaattgta
      661 tgaagattgc aacaatgctg tagacttacc cgcattcatt gcggaatagt agaaatatag
      721 ctgtgccatc agccacaggc cctcaatgat atgaaataaa gttggctgga gtttatcata
      781 attcgtcagt ttttcagctc aacagtatgc acagaagtgg gtatatgcgt tctcccttac
      841 gaagccttgc ataatccttc tgagctatca ttacaactgc ctcgcttgtt ataagcgggt
      901 aaatgactgc tggtaactat tcacaatctt taacctgttg cgcaagtaat agccctctgt
      961 tgacctccag gagatagtgc aatactaagt ccatgctctt attgcgactg ttctactttt
     1021 catcattcgc ttaataggga attctcgtaa acacaactaa tacagaagac tgaaaggtcg
     1081 tcagcctacg ataatctccc cataaaatgt gacatgaatc aggaagtttt aacctcacgt
     1141 gctgcgaaat catcggtgta aatagggcta tatgccgcgt cttttctggc taattttatg
     1201 aaaagatatt tattggcggc acaaaataaa gaacaatttt gaattcctta cattcctggc
     1261 tattgcacaa ctgaatttaa ggctctatta ttacctcaac aaaccacccc aatataagtt
     1321 tgagattact acaatgagcg aagcacttaa aattctgaac aacatccgta ctcttcgtgc
     1381 gcaggcaaga gaatgtacac ttgaaacgct ggaagaaatg ctggaaaaat tagaagttgt
     1441 tgttaacgaa cgtcgcgaag aagaaagcgc ggctgctgct gaagttgaag agcgcactcg
     1501 taagctgcag caatatcgcg aaatgctgat cgctgacggt attgacccga acgaactgct
     1561 gaatagcctt gctgccgtta aatctggcac caaagctaaa cgtgctcagc gtccggcaaa
     1621 atatagctac gttgacgaaa acggcgaaac taaaacctgg actggccaag gccgtactcc
     1681 agctgtaatc aaaaaagcaa tggatgagca aggtaaatcc ctcgacgatt tcctgatcaa
     1741 gcaataatct tttgtagatt gcacttgctt aaaatcccgc cagcggcggg attttttatt
     1801 gtccggttta agacaattta ataagaataa gctataaaaa aacggcgtcg attgctcaac
     1861 gccgtttcgt ggataacacc gatacggatg ttacttctta atgcccatct cttcttcaag
     1921 ccaggcttta aattccgtgc caagggtgtt atgacgaata ccgtattcaa cgaaggcctg
     1981 catgtaacct aatttattac cgcagtcatg gctcttccct ttcatatgat aggcttccac
     2041 cgtttctttt tcgatcagca tatcaattgc gtcggtgagc tgaatttcat caccagctcc
     2101 cggaggggtt tttgccagca acggccaaat atccgcgcta agtacgtaac gacccacaat
     2161 agcgagatta gacggcgcaa catccgcttt cggtttttct accacaccaa ccatcggtac
     2221 gctttcaccc ggcgctaatt caacgccttt gcaatccaca acgccatatg cggtcacatc
     2281 agcaaccggt tcaaccatga tctggctatg acccgtttca tcaaagcggc ggatcatctc
     2341 tgccaggtta tcctgtgaca aatcggattc atattcatcc agaataacat caggcaaaat
     2401 aacagctacc ggttcatcac ccactaccgg gtgagcacac aataccgcgt gtcccaggcc
     2461 tttcgccaga ccctgacgaa cttgcataat agtcacgtgc ggtggacaaa tagactgcac
     2521 ttcatcaagc agttgacgtt ttacacgttt ttccagcatt gcttccagtt caaaactggt
     2581 atcaaagtgg ttttcaatag agtttttaga tgagtgtgta accagcacaa tttcagtaat
     2641 gccagccgca atacattcat tcacgacgta ttgaattaat ggcttatcga caagtggcag
     2701 catctctttc gggatggctt tcgtcgccgg caacatcctg gttcctaatc ccgcaacggg
     2761 gataacggct tttttgactt tcgtattaat ggcagccatt taaattctcc tggactgttc
     2821 gtgttttgaa cgtgttcata tttctgtatc gcatcccagt atatcagcac cccgaagggg
     2881 ctaagttctg aaaaggaggc gagatagcat aattaagaca attacttata cgaatggcga
     2941 cgatagtacc accagccgac attagcaggt aatgcaaatt tagcccgcgt tatcgtttgc
     3001 tcattctgca gacaacatca agcgcagtcg accaccggtt ccccatattt ggcattgcca
     3061 ggcatcgcat cgctggctca attgattcaa ataagcgtta cctaaagtgc ctaacggaac
     3121 accattactg atttgcacct ggtgttcgcc ggtatttaac gtcgcattca gacccgcaga
     3181 aaccagaatg agatttttca gttcgcgatg ataatagcca actaatagcg gaaactgccc
     3241 cggcaaattg gcctgacgaa gtaaatggtt tacctgcttc aataacgcgc ccaactctgg
     3301 caaccgttga ttttggtgtg caagctgttc ctgtaataat ccgttaaaca atgcgcgtaa
     3361 taacaaggca gcaagtacgc cattatgtcc agctcgggtg acatcaaggc aataaaatgc
     3421 cagatcgttt tccgaaagtg cggcaatatc aagcaccagg ccgggtttgt ccgcggcaac
     3481 caattgacgg taattaaccc ggcaatggga aatcacctgc tgaaccggcg gttgtagttc
     3541 ctgtaataat ttcgccgctg cggcagggtt atcaaccatt gcatcccagt cgcgaaaaag
     3601 cctttcctct tcctcaacgc gcgaattaaa catgctggga tagagacagg caaaaaccat
     3661 ctcgcgcaag cgattcagat ctttaactgg tttcagcaaa acatcttcaa cgcccagacg
     3721 taacgctttg gcaatatctg ccatattttc agtggcagat atcaccagaa ctggggtctg
     3781 gtcgcctctg ttacgtatat gctccagcag tttaagcccg ttcattcgtg gcatcgcgat
     3841 atcacatatc atcaggtctg gagtgaaacc tcccagcaac tcaagggcat ccaccccatc
     3901 agccgccagt accgttgtcg ctcccaatga ggaaaaccat gaatccagaa gcgagcgaaa
     3961 tacctgctca tcttcaacaa tgagaatctg ttttccgacc aatggctgcg tcatgttctc
     4021 tcccctgact ggctttactc aatagtggca tgctattgcc acctgcgcct gtcagaatta
     4081 acttaagtgt agataaaaat tctggtcaaa tgttggtgcg taccaacggc aaaagttcgt
     4141 ccattttcct ttccactgcc agctgtccgg ccgcaatggc agcgtgcgcg cgatggaaat
     4201 caagcgtaga tatttgcggg caaacaggtt gaatcagaat atcgggcgga tcacctgcca
     4261 tgcggttcct tttaaggcgg ttctccaaca cctggataga cgtggtcata atctctgttg
     4321 ccgttggcgc tgtcacagcc cgacgtgtcg ttatgctgcc taacctttct ttcagacgcg
     4381 catgccacgg tagagaatca ccattctcgc tattttcttc actgacatta aaggagagca
     4441 gatcttgttg catcaaatga gcatcgtgct gcaggtcgac cgctatcaca atatcagccc
     4501 ccaatgcacg cgtaagagaa ataggaattg ggttaactac tgccccatca accagccagt
     4561 agccgttatg tgcaacgggt gccatgagtc ctggaatact gcatgatgcg cgaatagcaa
     4621 gatggagatc gccttcagta aaccataatt cacgccccgt acttaaattg gtggcaacag
     4681 ccgcaaagcg acgggaacaa ttttcgatct ctgtttccgg cattatttcg cgatattgat
     4741 tgaagacacg ctcgccgcgc agtaacccac cgcgctgcca ggagaggtcc atcaggcgta
     4801 aaacgtccca ataactgaaa gaggttaccc aatcttccag cgcagataat cgatcgcatg
     4861 cataggccgc gcccaccagc gaaccaattg aacatcctgc aacgatatca atttcaatac
     4921 ccactttttt tagcgcatta ataacgccaa tatgcgacca acctctcgcc gcgccagatc
     4981 ccagcgccag ccctattttt atctttctca ttattcccgc aagattcccc tggaatgcaa
     5041 tcgtagccac atcgctactg ctcggttaac atagtgctac tcaggcggtt aatacgccgt
     5101 tattgtttcc agggagatta tttgtgtctc agctttgtcc ctgtggtagt gctgtcgagt
     5161 atagcctatg ttgccaccct tatgtgtctg gtgaaaaggt tgcacctgat ccagaacatc
     5221 tcatgcgttc gcgttactgc gcttttgtga tgcaagacgc tgattattta ataaagacct
     5281 ggcatccctc ttgtggagca gcggcgttac gtgccgaatt gatgaccgga tttgcgcata
     5341 ctgaatggtt gggattgacc gtatttgaac attgttggca ggacgccgac aatatcggat
     5401 ttgtcagttt tgttgctcga tttaccgaag gtggcaaaac aggtgcaata attgaacgtt
     5461 cgcgattttt gaaagaaaac ggtcaatggt actatattga cggtacacgt ccgcagtttg
     5521 gtcgcaacga tccctgccct tgtggttcag gtaaaaaatt taaaaagtgc tgcggccaat
     5581 aatggttgac ggtacggttt agcaaacact ctcaacaagg tttttccagc aatgcattca
     5641 ctccaacgta aagttctgcg tactatttgt ccggaccaaa aaggtctgat cgcacgtatt
     5701 accaatattt gctacaagca cgagttaaat atcgtacaga acaatgaatt tgttgatcac
     5761 cgtaccgggc gcttttttat gcgcacggaa ctggaaggga tttttaatga ttccaccctg
     5821 ctggcggatc tcgatagcgc attgccagaa ggctccgtgc gtgagctgaa tcctgccggt
     5881 cgtcgccgga tagtgattct ggtcactaaa gaagcgcatt gccttggcga tttgttgatg
     5941 aaagccaatt acggcggtct ggatgtcgaa atcgcggcag ttattggtaa ccacgatact
     6001 ttacgttctc tggttgagcg ttttgatatt ccgtttgagc tggtaagcca tgaagggtta
     6061 agccgcaacg agcacgatca aaagatggcg gacgccattg atgcttatca acctgactat
     6121 gtggtgctgg cgaagtatat gcgggtatta acgccggaat ttgtggcacg cttcccgaat
     6181 aaaatcatca atattcacca ttcattcctg cctgcgttta tcggcgcacg tccttatcac
     6241 caggcctatg aacgtggtgt gaagattatt ggcgcaaccg ctcactatgt gaatgacaat
     6301 ctggacgaag gcccaatcat catgcaggac gttattcatg tcgatcatac ctacacagct
     6361 gaagatatga tgcgcgcagg tcgtgacgtc gagaaaaacg tcttaagtcg tgcgctatac
     6421 aaagtactgg cgcaacgcgt ctttgtttac ggtaatcgga cgattattct ttaatcgcca
     6481 gcaaaaataa ctggttacct ttaatccgtt acggatgaaa attacgcaac cagttcattt
     6541 ttctcaacgt aacactttac agcggcgcgt catttgatat gatgcgcccc gcttcccgat
     6601 aagggagcag gctagtaaaa agcattaccc cgtggtgggg ttcccgagcg gccaaaggga
     6661 gcagactgta aatctgccgt catcgacttc gaaggttcga atccttcccc caccaccatc
     6721 actttcaaaa gccccggaat tctcaaacga atctgcaatc aaatattctg cccaagcggg
     6781 gaaggatgag aagcttcgac caaggttcga cttgagcgcc agcgagagag cgttgccgca
     6841 ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca ccaccatcgc
     6901 ttccttgaat aatcaaaacc tgttactccg agctaatttt gcccgatggc accacgttta
     6961 ccgggcctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg acaactcacc
     7021 ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact aaaattcgtt
     7081 tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg tacgctccag
     7141 atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag caccaggtgc
     7201 agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg gaaggtcacc
     7261 accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg ctgagcggag
     7321 aacggaatgg tcagcaggcc cagcgcgcac tggataacga gcagcgacag gatcaggata
     7381 tccgctccgg tagtggttgc acgcacgcgt gggctgaaca tacgacgttt cagcaaaagc
     7441 acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc cattttctgt
     7501 ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcaacat accgaagaag
     7561 tgaccgacaa aaatccccag aataccgata tggaacaggt tcgacgccag gttcatccct
     7621 ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta gtcataacgc
     7681 agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc aaagaagaac
     7741 atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat tgcggcgcga
     7801 cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag cctttgtcag
     7861 caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc gtatcatcgc
     7921 gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta ttggccaatt
     7981 tcagcagcag atcaaacatc acggcataac ggctttcacg ctgttgcaga cgcgcgctca
     8041 gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt tgcggcagct
     8101 gcgacaggta ctccagatac agcggcagat ggtccggtaa ttcgcggctg tttaactgca
     8161 agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg tcacgggatt
     8221 caccgtgaac atgttcaaat agcaacagtg acgtggcacg gccacggtcg aacagttcgc
     8281 tgtactggac ctgggcatcg agcggatcca tcgccgttaa atcgcgcagg aaaatgccca
     8341 gcgcatgggc atcttctttc gacagatttt tcgacgcagc aatcgcctca aacatctctt
     8401 gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca atcacgagtt
     8461 cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac gacggctgtt
     8521 gaacagattg aatttggtat ctgagccgtg gcagccatca ccaaaggtaa agccgcagcc
     8581 atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca ccacaaagcg
     8641 atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg cttcggtcag
     8701 accgacctct tccagcgcac gggtatcaac tttaccgtca acagtttcag cacgtttgta
     8761 atgacgcatc gccagcatac gtttcagcgc acgcagtacc ggtttggtat caccggcggt
     8821 cagcagattc gccagatact gtaccggaat acgcaagctt tcgacgtctg gcagaattcc
     8881 gttgctgccc agctcgcccg cgtctgctgc agactgaatc ggagacagag gcggcacgta
     8941 ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt tccattccat
     9001 cgccatttta taaaccggcg actgctgtgc agcttcaata acgctcagcg gaataccgtc
     9061 tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacgt ccagctgacg
     9121 ctggtagaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta
     9181 cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca cggtcggctg
     9241 acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc tcttccagtt
     9301 gaagtagatt tttttgtacg ggcatccggt gatgcacata cgccagccac ggcatttatc
     9361 ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt
     9421 cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca tcataaaggt
     9481 gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt ctttcgccag
     9541 tttatcaaac tcaccgccca ggtcatcttc ccagttcggc cctttttcga ttttcgccat
     9601 ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg
     9661 cgcggtatgc aggttctgat aatcaaaatc gaacggctcg taataatcgt cgatccccgg
     9721 cagatgcggg ttagcaaaga ttttacccag cagcatggct cggttaccca tgcgcggttg
     9781 cagtttgcca ttgattttac ggatccagcc gcctttgtat ttttcctggt tttcccagtc
     9841 agtcgggaag ccctggcccg gcttggtttc cacgttgttg aaccacgcgt attccacgcc
     9901 ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc cgatgcactt
     9961 atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct ctcctgtacc
    10021 tggtcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac tacaacaaac
    10081 tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag atgggcatag
    10141 ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg gataccacca
    10201 cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc gtggtacatc
    10261 atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag tgcaccgttg
    10321 ctgttgaaga cttcaatcca gtcgttatcg gcgataccca gatctttggc atcggcttca
    10381 ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt gtcgctgtag
    10441 gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg
    10501 ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg cggacgataa
    10561 accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag ttgctgacga
    10621 ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa
    10681 acgtgttcat cttccagacc agaccaggtc gggctggaga taattttgcg cggctgtgcc
    10741 tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg cgtatggtca
    10801 cgaccggtaa attcgctcag ggcagcccag gctttcacgg ctacctgacc gttggtttcc
    10861 ggtgccagcg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat cggctggcct
    10921 tttgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat ctcgctctgg
    10981 gtgttccagg caatcccttt accgccgtta ccgattttct ccatcagcgg gccgatagag
    11041 gtaaagcgtt cgtaagttgc cggataatcg cgctctacga ccataatgtg cggtgcagtt
    11101 ttgcctggga tcaggtcgca ttcgcctttt ttccagtctt tcacatccag cggctgcgcc
    11161 agttcagcgg cagagtcgtg ctggataggc agcgtgacga tgtcggtttc tttacccaga
    11221 tagccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc ccagtcgctt
    11281 ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg atgcatatcc
    11341 gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag
    11401 agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt gccttccaga
    11461 ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgttgccc cagatcttta
    11521 ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg acctttaccg
    11581 gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt tttaccgttt
    11641 tctggttgtt ctgccgcaaa acggatggaa ccctctttca gggatttcac cgtatagtcc
    11701 accggattca tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac
    11761 tgcggtgcag acggcagcca gcccatgcgt tccgcacgga cgttaaagtc gatcaagtgt
    11821 ccggtatagc gggatttgtc cgccatcggt gacagcaact cttccgccgt gacggtttca
    11881 taacgccact ggctggagtg gttatagaaa taagaagtgc tgttcatgtg acgcgccgga
    11941 cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg cagtttttcc
    12001 tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca gaaaatcagc
    12061 atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag ccccgcaccg
    12121 acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc acgggcgatg
    12181 cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg ggtataagct
    12241 ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc cagaccgtag
    12301 tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc agccagttgc
    12361 aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt gttgaagtgt
    12421 tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc ctggctaccc
    12481 agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc
    12541 catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac catttcgcca
    12601 ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag cgcatcaacc
    12661 agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc ttccagcatc
    12721 accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg gcttgggttg
    12781 tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc cgcatcggtg
    12841 ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta gtctggtgtg
    12901 acggcaacag ttttggtgcc tttgtaacgc acttcagtaa agaagtgagc atccggggta
    12961 cgcgtctgcg gcacgtttga accccaggcg atgatgtagc tggagttata ccagtcagca
    13021 gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg caggtcgcag
    13081 taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg tgcacccgat
    13141 gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg gtccgggccg
    13201 tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc ctgccaagaa
    13261 gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct
    13321 tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac
    13381 attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact gtaaagatac
    13441 caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag atccggacgg
    13501 gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt
    13561 ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc
    13621 tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc
    13681 ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc
    13741 atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta
    13801 agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag tgatggggat
    13861 gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagtta cagcataaca
    13921 tgctgtaaat taagacgttt tatacctata gttatttaaa ctattaagat ttttatgcaa
    13981 atgggtatta aggagtattc cccatgggta acatattaat ggctcttaat gaaatccggc
    14041 gaaataagcc atataacact aatatgttgt aactaaatga cgcataaaaa aagcgcggca
    14101 tcatgccgcg caaaggataa tcaaatatta agatttattt tttagaatgc cgaccatata
    14161 ccgcccaagt aatcactacg caggcgatat agaaaatcaa aaatactttc attgcaccta
    14221 ctggcgaacc cgttaatgcc agcgagctac caaacgcttt cgggataaag aagccaccaa
    14281 tcgcgccaat cgcagagatg aaacccagcg ccgctgccgt gccggttgcc gcttcacgca
    14341 tcgcacgttc gtcagaaccc ccttctgctt tcacgcgatc cattgtcagt ttacggaaga
    14401 tcactgaaat catctggaaa gtggaaccac tacccagccc agctgtcagg aacagcgcca
    14461 ggaagaccgc gaagaacgcc atgaagcttc cgccctgccc gtcagtcggt aaggtcagga
    14521 acagcaggcc gctgaaaatc gccatcagaa taaagttcac cagcgtgaca cgagttccgc
    14581 ccagacggtc agataatgca ccacctgcag aacgcgccag cgcaccaata aacggcccga
    14641 agaaagcgta ttgcagaatc tgaacatccg ggaactgcgt ttttgacagc atcgcaaagc
    14701 ccgcggagaa gccgatgaag gagccgaagg ttgccagata cagcaggctc ataatccaca
    14761 gatgaccccg tttgagtacc ggcaactgct ccttgatgga ggctttcgag gtagcaagat
    14821 cgttcatgcc aaaccacgcc gcaatggtga agatggcaag gaacggcacc catatccagg
    14881 acgcattcgc cagatacagc tcagtcccat ccggctgttt gacgccctgg ctaccaaata
    14941 ctgcgaaaat cgacagtgat accaccagcg gagcaaccaa ctgcatgacg ctgacgccca
    15001 tgtttcccag accaccattc agacccagcg cgccaccctg cttctgtttc ggaaagaaga
    15061 agctgatgtt tgccatactg gatgcgaagt tcgcgccagc aaagccgcat agcagagaga
    15121 tgatgatgaa gacgctataa ggcgtggagg tatcctgcac ggcaaaaccc agccagacgc
    15181 aaggaataat cagaataccg gtgctgaacg ccgtccagcg acgaccaccg aagataggaa
    15241 ccataaagga gtatggaaca cgtaataacg cgccagaaac cgaaggcagc gcagtcaaca
    15301 taaatagctg atcggtcgta aaattaaaac cgactttcgg taagttcacc gcaacagcgc
    15361 tgaacaacat ccatacgcaa aacgccagca gcagacaggg aacggaaatc cacaggttgc
    15421 ggctggcaat acgttgaccg cgttgttgcc agaacgcagg atcttccggt cgccaatctg
    15481 ttatgacagc tccagtagcc ctttcggagg cagatgagtg actcatagac acctctgata
    15541 ctcgtttcga tgtctgccac cttagtgtct ggagctaaag gcaatttgat gtaaatcaaa
    15601 cgataagata actttatcta tcattgatat ttatcattac ccatagtgag tacagtgatt
    15661 tcataaaaat tatgagattt ttcacggtgc tgtagaatcc ctacccttac cgatgtaaag
    15721 cgactaacca cacggcaaat aaggagtaac tctttccggc tatgggtata cttcagccaa
    15781 tagccgagaa tactgccatt ccagaatata tcgtcacatt cattaaggtt attgctcatt
    15841 taaagcctga aggaaggggt ttacatgctt aaacgttgtc tctctccgct caccctggtt
    15901 aatcaggttg cgcttattgt gttgctttct actgctattg gactggcagg gatggcggtt
    15961 tctggctggc tggtgcaagg cgttcagggc agcgcccatg cgatcaacaa agcgggatcg
    16021 ctgcgcatgc aaagttaccg tctgttggcg gcagtgccat taagcgagaa agacaagccc
    16081 ttaattaaag agatggaaca aacggcattt agcgccgagt tgactcgagc agcagaacga
    16141 gacggacaac tggcgcaatt acagggctta caagattact ggcgtaatga actgatccct
    16201 gcgctgatgc gtgcacaaaa ccgagaaacg gtgtcagcgg atgtcagcca gtttgttgcc
    16261 gggcttgatc agctggtatc tggttttgac cgcaccacgg aaatgcgtat tgagacagtg
    16321 gtactggtcc atcgggtaat ggcggtattt atggcacttt tactggtgtt cactattatc
    16381 tggttgcggg cgcgactgct acaaccgtgg cggcaactgc tggcaatggc gagtgccgtc
    16441 agccatcgcg attttaccca acgcgcaaac atcagcgggc gcaacgaaat ggcgatgctt
    16501 ggaactgcgt tgaacaatat gtctgcagaa ctggccgaaa gttatgccgt acttgagcag
    16561 cgggttcagg agaaaaccgc cgggctggag cataaaaatc agatcctctc ttttttatgg
    16621 caggctaacc gccgtttgca ttcccgcgcc ccgctgtgtg aacgcctgtc accggtactc
    16681 aacggcttac agaatttaac cctgctacgt gatatcgaac tgcgggtgta tgacactgat
    16741 gatgaagaga atcatcagga gtttacctgc cagccagata tgacttgtga tgataaaggc
    16801 tgccagctct gcccgcgcgg cgtattaccc gttggtgatc gcggcacgac cctgaagtgg
    16861 cggctggctg actctcatac gcagtacggt attttgctgg cgaccctgcc gcaggggcgt
    16921 catcttagcc atgaccaaca acaactggtg gataccctgg ttgaacaact taccgccacg
    16981 ctggcgctgg atcgccatca ggaacgtcag caacagttga tcgtgatgga agagcgtgcc
    17041 accattgcgc gcgaactgca tgattctatt gcccaatctc tctcttgcat gaagatgcag
    17101 gtgagttgtt tacagatgca gggcgatgcg ctgccagaaa gcagccgcga actgttaagt
    17161 cagatccgta acgaactgaa tgcatcctgg gcgcagttgc gtgaattgct caccacattc
    17221 cgcttgcagc tcaccgagcc tggattacgt ccggcgctgg aagcgagttg cgaagagtac
    17281 agcgccaaat ttggcttccc ggtgaagctg gattatcaat tgccgcctcg tctggtgcct
    17341 tcacatcagg caatccactt gttgcaaatt gcccgtgagg cattaagtaa cgccctcaaa
    17401 cattcgcaag cgagtgaggt cgtggtgacg gtggcgcaaa acgataatca ggtcaaactg
    17461 accgtccagg ataacggctg tggcgtgcct gaaaatgcca tccgcagcaa tcactacggc
    17521 atgataataa tgcgcgaccg tgcgcaaagt ttacgaggcg attgccgcgt ccgccgtcgt
    17581 gaatcaggtg gcaccgaagt ggtggtcacc tttattcccg aaaaaacttt cacagacgtc
    17641 caaggagata cccatgagta atcaggaacc ggctactatc ctgctgattg acgatcaccc
    17701 gatgctgcga actggcgtaa aacagcttat cagtatggca ccagatatca ccgtggttgg
    17761 cgaagcgagt aatggcgaac agggtattga actggcggaa tctcttgatc ccgatctgat
    17821 cctgttagat ctcaatatgc ccggcatgaa cggtctggaa acgctggata aactgcgcga
    17881 aaagtccctc tcagggcgca ttgtggtatt cagcgtctct aaccatgaag aagatgtggt
    17941 caccgcactg aaacgcggcg cggatggcta tctgttaaaa gatatggaac cggaagatct
    18001 gctgaaagca ttgcatcagg cagctgctgg cgaaatggta ttaagcgaag cattaacgcc
    18061 tgttctggcc gccagcttgc gcgctaaccg tgccactact gagcgcgatg ttaaccagtt
    18121 aaccccacgc gagcgcgata ttctcaagct gattgcccag ggtttgccga acaagatgat
    18181 tgcccgccgc ctggatatca ccgaaagcac agtaaaagtg cacgtcaagc acatgctgaa
    18241 gaaaatgaag ctcaagtctc gcgtggaagc agcggtatgg gtgcatcagg agcgcatttt
    18301 ctgattacgg ttcccagcgc agttcgtcgt ttgacaatgc atcgaacggt tcgacaagcg
    18361 ttagcgtgat ctcattggag gagacacgct gcccctggtt atcttccacc accacagaca
    18421 atcgccagtg attgcttgcc ccttccccgt tctgccagtc aggcatgatc agcgtccagc
    18481 cctccgcgct gttggcttgt gcgcccggcg tcaaacttaa tatctgcgta tcgccctgcc
    18541 aaatcagttg ccgaataccg taacgactgc ggatttgtaa tttcagcggc actgtttcgc
    18601 caggttttag atcccacggc ggtgtcgcca gaaacaccgt taacgttttt cgctgtcggt
    18661 actcaagagt cggtagatta tttcgctgcg gattgtcata gcgactacca cgtaacgact
    18721 gactttcggc aacctcgccc gcagaaagtt gttttttgag cggtacacca aagcggtaat
    18781 taagattcag cccgaggtta ttttgatttt cgccactttc cccctgttta tgctgggccg
    18841 tcacagtgac taatggcaca ggggtgtaat ttaatcccag actcaacgcg acgggattgt
    18901 gataacccgt accagagtta aacaaatcaa cacgatcacc aaaatactgt tctaggctga
    18961 cactggtatt gaggtgttga tagaacggca tgcgcatccg agctgtcagg tcgtacccgc
    19021 gcgccatccg ttgttcctgc gtggctgtct gttcatgcca tgcagcaaac ggctgataaa
    19081 agtttgccga taatcgcaaa tattcgcccc acgcttcggc accaaagccc gctcgctgaa
    19141 gattttcgtc ctgcaagttg tcataaaaag tgttataacc caccagccag ttgccgcgcg
    19201 cccagcgttg cccaacgccc acattgctca ccaacccatt atcctgctga gtaagaccaa
    19261 gctggctcca ggtgagataa cgatcattat cttgtaacgg cacaaaccag cttccacgac
    19321 tgccggtgaa atgtccttcg ttatccactt tgacgtcaac actggcattt ccccacggtg
    19381 atagccagga ctctacgtgc tgattaacct gttgactaag cgcgtcgcgg acttttccca
    19441 atgcgaaagc ttttgcctgc tcgccagtat ccagcccgtt atcattcata ctggtttcgc
    19501 cgaaatcttt cacaatttca gcaaagtgtt tttccccatc atgattttcg ggagccatgc
    19561 ctaaatccgg cagaccatca ttgttattat caaagggatt tgccgctttt tgctcgaagg
    19621 tagattgtgc gttagctgta ccgcctgcta caagcaagag taaataaaac gggataatgc
    19681 gaggaacgaa acggctcaaa acgtcggtaa cttccggtaa taatgaaaaa ccatagcctt
    19741 aaagaataac gattactggc aacttttgtc gtattttctc gcgctttcag gaaaatcctt
    19801 aaaaatgtga ccgattggtc agacgctgca gaagcagtaa gatagaagat gagtgtctaa
    19861 ttccctgaat acaggagtaa ataatgcaaa aaatagtgat cgttgccaat ggcgcacctt
    19921 acgggagcga atccttgttt aacagcttgc ggctggccat tgcgttacga gagcaggaga
    19981 gcaatctgga tctgcgtctg ttcctgatgt ctgatgcggt cacagccggg ttgcgcgggc
    20041 aaaaaccagg ggaaggctac aacattcagc aaatgctgga gatccttacc gctcagaatg
    20101 taccggtgaa attgtgcaaa acctgtaccg acgggcgcgg gattagcaca cttcctctga
    20161 ttgatggggt ggaaatcggt actcttgtgg aactggcgca atggacgctg tcagccgata
    20221 aagtgctcac attttaatgt ttaagggggc gtctgacgcc ccactatgag ggaagtttac
    20281 tcaggcgaat cccggcctta acacaccatc cggataattc tccgccagta gtttttttac
    20341 cgataccagc aaatcattca gcccatcgtc ctgcattccc agtttgataa gctcctgttc
    20401 cagtgaaaac aggtattgtg cattggttcc cagcggaccg ctcgccgccg caatcaacgg
    20461 cgcgatgacc tgagcgcgag tgtcagattc atattctgga tgtcgcgggt ccataataaa
    20521 cacaatggcg tttactgtgc gtccatcatc aagatcaagc tgacaccagg ttggcagata
    20581 acagccggta atcatctcgc gcttccacaa cagggttagt tcctgctcca gcgtctcttc
    20641 tggtagtcga taggcgacgc ctgtggtgcg tccgccctct ttcagtgcaa gcatccgtcc
    20701 cggctggtgc gcagtcccgc gcccggcggt caggcgcagg caaaacgcgc gatgccatcc
    20761 aaccagtgta ccggtgcatg attcggtaaa ctccagtgcc ggattccaca tcaacgatcc
    20821 ataaccgaag atccacaccg gtccctcatc aggtcgacaa gccagcgtcg ccgccagcga
    20881 agccgcccgt tgttctgctg accataagag tgattcttca atggcaccaa atgccgtttt
    20941 acaatcggca ttcatcaaga aatcacgcgt tatcacctta cacctccgcc actgcatact
    21001 tccctgcgac aactttttgg tgtcttccgg acatttttac cgctcatttt gaaaccacta
    21061 acggaacaat atgcaatcca cggaccactt gcaaggcaac accgcacgaa tagctttaac
    21121 taagctaccg gttttacgat tttttatgcc atttatcatc atcccctttg gcatattcat
    21181 gcttcacagc agcccaggcc actttatgcg cggtttcttc gcgactggcg tcatcacgcc
    21241 gatcttcttt atctttatat tgatcccatg cgctgttgaa cgcttcttta tagatatcct
    21301 gggcatgaga cggtagaacg tgctttacgc tttccggcag atcgcttttc gttttatacg
    21361 gcatcgttga ctccttttac aaagttgaac attaagtgtg gtcaacaata tctactcttg
    21421 ccaaaacata agaattttca tgtcagactg ttcttaagaa acgctaaaac aaacattgtc
    21481 taaacgtaat acttattttt atgccccaat gagtaaaaaa tcgacagcgg ggtaaaaatt
    21541 cgtaaaatgt taccgatatc cagacacttt ctgttatttt tcctacctat tttttacatt
    21601 ttcgataaat tcatctgcaa agggataacc ataatgtcaa atgctcaaga ggcggtaaaa
    21661 acccgccaca aggagacttc gcttattttc ccggttctgg tgctggtagt gctgttcctg
    21721 tggggaagta gccagacact accagtggtc attgccatca atcttcttgc gcttattggt
    21781 attttaagta gcgcctttag tgttgtccgt catgcggacg tattagccca tcgcctggga
    21841 gaaccttacg gttcgcttat tcttagcctt tcagtggtta ttcttgaagt cagtttgatt
    21901 tcagctttaa tggcaaccgg cgacgccgcg ccaacgctaa tgcgtgatac gctctattca
    21961 atcattatga ttgttaccgg tgggctggtt ggcttttcat tattgttggg cggtcgtaag
    22021 tttgccaccc aatatatgaa tctgtttggt atcaagcagt atttaattgc gctgttcccc
    22081 ctggcgataa tcgtactggt atttccaatg gctctgcctg cggcgaattt ttcaaccggt
    22141 caggcgttac tggtagcatt aatttctgcg gcaatgtatg gcgtattttt gctgatccag
    22201 gccaaaacgc atcaaagttt gtttgtctac gagcacgaag atgacagtga tgacgacgac
    22261 ccgcaccacg gtaaaacgtc tgcccatagc agcctgtggc atgctatctg gttgattatc
    22321 catctgattg ccgttattgc agtcaccaaa atgaacgcca gctcgctgga gacattgctc
    22381 gacagcatga atgcccctgt tgcctttact ggcttcctgg tggcactgtt gattctgtcg
    22441 ccggaaggtt taggtgcgtt aaaagcagtg ttgaacaacc aggttcagcg cgcgatgaat
    22501 ctgttctttg gttcagtgtt agcaacaatt tcgcttaccg tacctgtcgt cacgctaatt
    22561 gcctttatga cgggtaacga attgcagttt gcacttggtg cgccagaaat ggtggtgatg
    22621 gtggcctctt tagtgctgtg ccatatctcc ttctccaccg gacgtactaa cgtgctcaat
    22681 ggcgcagcgc atctggcact gtttgccgcc tatttgatga cgatatttgc ctgacagtta
    22741 tatctcctcc gatgacggaa atcgggagag ataagataaa ttcctgctta ttgtatttct
    22801 tcatactacc tggacagctc gttaatcaga attgcgagcc tgtaccacaa cctgcacgaa
    22861 accactgcaa cagctgttga aaagccggaa tgtactcttt attgcaaaca tttcagggat
    22921 gaagtgaaga tgaattgaga cgtagtatcc acaccaagcc ggaattatcc ggtgaggcgc
    22981 aatgttgcgg gggctttatc cctggtggca ttggttgctg gaaagagaaa acccccgcac
    23041 gttgagaggt aaaacctgac aacaaaacgg gggctaatct tgaaaccaga cacttcaaga
    23101 atagccgcaa aatgttgtca ttacaacaag gcggctatat gacgctcgcg cagtttgcca
    23161 tgactttctg gcacgacctg gcagcaccga tcctggcggg gattattgcc gcagcgattg
    23221 tcagctggtg gcgtaaccgg aagtaaggtg aaaacgggcg tcaggctgcc gtgatggcaa
    23281 tatgcgcccg aaaccggccg caggggtaac agcctgcggc cttttttgta tctggcaccc
    23341 atgaaaaaag tcttaacgca gaacgctaag actttatttt tcaagcaaaa aagattactt
    23401 gctggtatcc agttcttcga aacctttcac cagatcatca atcgctttca tctgcttgag
    23461 gaacggttcc agtttagcca gcggcagcgc ggatggacca tcacatttcg catgttccgg
    23521 atccggatgc gcttcaataa acagccccgc cagacctacc gccataccgg ctcgtgccag
    23581 ctcagccacc tgagcacgac gaccaccgga agctgcgcca aacggatcgc ggcattgcag
    23641 tgcgtgggtc acgtcgaaaa tcaccggcga gttaccagac actttcttca taatgctgaa
    23701 gcccagcata tcgacaacca ggttgtcata gccgaagtta gcaccacgat cgcaaagaat
    23761 cactttttcg ttgccgcctt ctttgaattt atcaacgata ttacccatct gtcccgggct
    23821 gacaaactgt ggtttcttga cgttaattac cgcaccggtt ttcgccatgg cttcaaccag
    23881 gtcagtctgg cgagcaagaa acgccggcaa ctgaatcaca tccacgacat cagcaacggg
    23941 ctgtgcctga cttggttcgt gaacgtcggt gataattttc acgccaaaag tctgcttcaa
    24001 ctcctggaag attttcatcc cttcttccag gcccggtcca cgataagagt ggatggagga
    24061 gcggttggct ttgtcaaaag aggctttgaa cacgtaaggg atacccagtt tctgggtcac
    24121 agttacgtag tgctcgcaaa tgcgcatcgc cagatcgcga gattccagca cgttcatacc
    24181 gccaaacagt acgaacggca ggtcatttgc tacgttgatg tcgccaatgc taaccacttt
    24241 ttgtttcata ggatcgcctt aatcttgagt aaaatgtcga ttaattaatg cagcacaata
    24301 tgtttatgcg cgatgttatt tatttgcgca cggatcattt cgctgatcgg gtcttccgga
    24361 caccgttcaa cgaaataact taaatcgttc aacgcaacgt gttcgcaatc cagttgcgca
    24421 taaatcaacc cacgatcgcg aatttcatag ggatcttcag ggttgaattg taataaagct
    24481 tcgctggtgc gtaacgccag ctccatctga ttttcttcca tcaacgaggc tttgagtgta
    24541 tccagcaatt tgcggattac ctcaatgtta tcagcttcat caaggtcttc ataaaacagt
    24601 tccgccgacg ggctgatatt tccctttaac catccgtcca gcatatgttc gcttaacgat
    24661 tcaccgttaa aaggattaat cagccaaatt tcgccatccg gacattcaat gcgcaatatc
    24721 agctgcgtag ggaaaatcac cggcagcagc ggcaaatcga gacgattcgc gacccataat
    24781 aaaaccgcac ctaatgatac cgcactgccc tgtcgattct ttaacacctg gtccagccat
    24841 aatgcatcgg aaagacgata aacgccgcgt gaggctttaa atccccagtc gccgtaaaac
    24901 agcgcgatca gtttttccag ctgctcttct aaaggcagaa gctggctgat ttcttccttc
    24961 gccagactaa cgagacgctc cagttcgtcg taaacatctt gcgagggaaa atcgcggcgg
    25021 attgcttcgc aagccaggat catgccttcg cacaatggcg ctttattaaa ttcgaaatca
    25081 gctaacgatc tcatgactta ccccagtaac ggtacttttg tggtggcgag tttaatgatg
    25141 atgtacagca ccaccaacgc cagcgggaag gcaataatgc gcgcctgctg actgtgcata
    25201 cgacgataat cgagtgcaat aaaacccaaa acgatataaa tgataactcc aaacagcttt
    25261 tcagtcagcc actgtgcctg cccggaaaat ggcaggatgt gcgctttagc catcaacgca
    25321 atgccgctta acaataacac cgtatcgaca accggcggca caatgcgcgt ccagcgagca
    25381 aatgcctgtg gatgcttctg ataacgtagc cagaaacgta aggttaatag cccaacagaa
    25441 agcgcgatac taataagatg aacactaagc agtgtagaaa aacttgtcat tgataatagc
    25501 ggccgagcgt tacgcgctcg ttatcaccat agtcacggca ggtttcgacg tcatgatacc
    25561 ccgcgaggat aaatgcttgt cgcaccgctt cgccctgctg ccagccatgt tccagaagca
    25621 gaaagccgcc ggataccagc gcgttacgcg actgttcgat gatatgcacg atgtctgcca
    25681 ttccactgtc tgccgcaacc agcgcagtga gcggctcaaa gcggacatcg ccttgttgaa
    25741 gatgtgggtc ctgctcgtca atatacggcg gattgctgac aatcatcgca aactgctgcc
    25801 cggctagcgc gctaaaccag tcgctttgca gaatgtggat atttttgatc gccagatgct
    25861 gggcattacg ttgtgccagg gagacagcat caggcatacg atctacagcg ataatttcgc
    25921 agtccgggcg ctcgctagcc agcgcaagcg caatcgcccc ggtacccgtc ccgagatcga
    25981 gaatacggca aggttgttca ggcaaccgcg ccagtgcctg ctccaccaga cactccgtat
    26041 ccgggcgcgg aattaaggtc gctggcgaaa caaataacgg caacgaccag aattctcgca
    26101 ccccggttaa atgagcaatg ggttcaccat cgcgacgacg tgtcagtagc gcatcaagtt
    26161 gctgacattg ttcgtcagtc agctgcgttt caccaaaggc gagaataaaa gtacgccctc
    26221 tgccggtaac atgctccagc aggatttcag catcacgccg cgggctttcg ctcgcctgaa
    26281 gttggcttat tgcttcacgt aaccagtgtt gatattccat tattcctgct cggacaacgc
    26341 cgccagttgg tcggcctgat gttcctggat aatcggttca atcagcatat ccagcttacc
    26401 ttccatcact tcatccaggc ggtagagcgt caggttgatg cggtgatcgg taacgcgccc
    26461 ctgcgggaaa ttgtaagtac ggttacggtc gctgcgatcg ccactcccca gcaggttacg
    26521 acgggtagac gcttcggcct gttggcgttt tgccatttca gcagcgtgga tgcgagcacc
    26581 gagcactgaa agcgccttcg ctttgttttt atgttgtgaa cgttcgtcct gacattcaac
    26641 aacaatcccg gtcggcaagt gagtaatacg aattgccgaa tcggtggtgt taacgtgctg
    26701 accacccgcc cctgacgagc ggaaagtatc aatgcgtaaa tctgctgggt tgatgtccgg
    26761 cagttctgcg tcaggcagtt ctggcattac cgcaacggta caagcagaag tatgaatacg
    26821 accctgcgat tccgtagcag gaacacgttg cacgcgatga ccgccggatt caaatttcag
    26881 acgaccatac acaccatcac cgctaatttt ggcgatgatc tctttataac caccatgttc
    26941 accctcgctg gcgctcatga tttctacccg ccagcggcgg gcttcggcgt aacggctgta
    27001 catacggaac agatcgcctg caaacagcgc tgcttcgtcg ccgccggttc cggcacggac
    27061 ttcgaggaag gcgttacgtt cgtcatcagg atcttttggt agtaatagaa cctgtaattg
    27121 ctgttccagt tgctcgcttt tttctttagc ttcgcgcagt tcatcctgcg ccatctcacg
    27181 catttcagga tcgtcgagca tcatctgtgc ggtttcgata tcttcctgaa cctgttgcca
    27241 gtcggtaaaa cagcgcgaaa catcacttaa ctgcgcatat tcgcgtgata atgcgcgaaa
    27301 acgttcctgg tcggcgatag tttgcgcgtc acccagcaac gcctgaactt cttcatggcg
    27361 ttcatgcagg gcttccagtt tggcaacgat agaaggcttc ataggcgtaa atgcaccctg
    27421 taaaaaaaga aaatgatgta ctgctactcc agcccgaggc tgtcgcgcag aatattcagg
    27481 cgttcgttat ccccgtcacg ggcggcctgt tgaagtgatt tcgttggcgc atggatcaag
    27541 cggttagtca gtttccatgc cagatcctgc ataatggctt gcgcgtcgcc gccctgctca
    27601 agggccgcta acgctttggc ggttaactca tcgcgaacgt gctctgcctg gctgcgatac
    27661 tcgcgaatgg tttcgctggc gctttgtgct cgcagccacg ccataaattc gctggtttcc
    27721 tgagcgacaa tagtttccgc ctcaaccgct gcggctttac gctgcgccag gttgtgcgaa
    27781 atgatgcttt gcagatcgtc cacactataa agataagcat tcgccagttt gccaacttcc
    27841 ggctcaacat cgcgcggaac ggcaatatcc accaacagca ttggttggtt acgacgactt
    27901 tttaatgcgc gctccaccat gcctttcccg ataatcggta acgggctggc ggtggaactg
    27961 atgatgatat cggcttcgcg cagacgttcg tcgatctcac tcagggcaat cacttccgcg
    28021 ccaacttcat ctgccagaat ttgggcacgt tcgcgagtgc ggttggcgat aatcatcttc
    28081 tgtactttat gttcgcgcag atgacgcgct accagctcga tggtttcgcc cgcgcctacc
    28141 agcaacactg tgaccgtaga gagcgattca aagatctgcc gcgccagcgt acaagccgca
    28201 aaagcgacag acacagcact ggcaccgata tctgtttcag tgcgaacgcg tttcgctaca
    28261 gagaaagatt tctggaacat gcgttccagt tcgctggcct tcatatgacc tttttgcgaa
    28321 tcgacaaacg cttttttaac ctgaccgagg atctgcggct ccccaagaac cagtgaatcc
    28381 aggccgctgg caacacgcat taaatggcta accgcgtcgt tatcctgatg ccagtagagg
    28441 cttttacgca gatcttcttc attaagattg tgataatcgc aaagccagcg gattaacgcc
    28501 tcttgcaggt tatcctgctc ttcaacgcta agataaagtt ccgtgcggtt gcacgtcgac
    28561 agcaccacgc cgccctgcac catcggctgc gcaagcaggc tgtcaagcgc ctgatcgagc
    28621 ttatccggcg aaaacgatac acgttctcgc agcgatacag gtgccgtttt atggttgata
    28681 ccgagtgcta aaagggtcat gtctgcggga aataatacca acgttgatag ggttagtctg
    28741 cttgcatcat acaggatgcg taagatcaat aaaagagagc gccccctttt ggagtaattg
    28801 ccgaaagccg ttagattctg gcaattaaga caacttgaac atagacgata gcggacggta
    28861 acgctagcat taagggttat aactgcaacg tatctcaagg acttgtcatc actatgcccc
    28921 tgcccgattt tcgtcttatc cgcctgctac cgctggctgc tcttgtgctc actgcctgtt
    28981 ccgttaccac gcccaaaggt cctggcaaaa gcccggattc gccacaatgg cgtcagcatc
    29041 agcaagacgt gcgcaatctt aatcagtatc agactcgcgg cgcgttcgct tatatttctg
    29101 accaacaaaa agtgtacgcc cgctttttct ggcagcaaac cggccaggat cgctaccgtc
    29161 tgctgctcac taacccattg ggcagcacgg aactggagct gaatgctcaa ccgggtaacg
    29221 tgcagttagt cgacaataaa ggtcagcgtt ataccgccga tgacgccgaa gagatgattg
    29281 gcaaattgac cggaatgcca attccgctca acagcttgcg ccagtggatt ttaggtttac
    29341 cgggtgatgc aaccgactac aaactggacg accagtaccg cctgagcgaa attacctaca
    29401 gccagaatgg caaaaactgg aaggttgttt atggtggtta tgacaccaaa acgcaacctg
    29461 cgatgccagc caatatggaa ctcaccgacg gtggtcaacg catcaagtta aaaatggata
    29521 actggatagt gaaataatgc ggacacagtg gccctctccg gcaaaactta atctgttttt
    29581 atacattacc ggtcagcgtg cggatggtta ccacacgctg caaacgctgt ttcagtttct
    29641 tgattacggc gacaccatca gcattgagct tcgtgacgat ggggatattc gtctgttaac
    29701 gcccgttgaa ggcgtggaac atgaagataa cctgatcgtt cgcgcagcgc ggttgttgat
    29761 gaaaactgcg gcagacagcg ggcgtcttcc gacgggaagc ggtgcgaata tcagcattga
    29821 caagcgtttg ccgatgggcg gcggtctcgg cggtggttca tccaatgccg cgacggtcct
    29881 ggtggcatta aatcatctct ggcaatgcgg gctaagcatg gatgagctgg cggaaatggg
    29941 gctgacgctg ggcgcagatg ttcctgtctt tgttcggggg catgccgcgt ttgccgaagg
    30001 cgttggtgaa atactaacgc cggtggatcc gccagagaag tggtatctgg tggcgcaccc
    30061 tggtgtaagt attccgactc cggtgatttt taaagatcct gaactcccgc gcaatacgcc
    30121 aaaaaggtca atagaaacgt tgctaaaatg tgaattcagc aatgattgcg aggttatcgc
    30181 aagaaaacgt tttcgcgagg ttgatgcggt gctttcctgg ctgttagaat acgccccgtc
    30241 acgcctgact gggacagggg cctgtgtctt tgctgaattt gatacagagt ctgaagcccg
    30301 ccaggtgcta gagcaagccc cggaatggct caatggcttt gtggcgaaag gcgttaatct
    30361 ttccccattg cacagagcca tgctttaagc cgggcaagct gagtttcggt gacaacgtca
    30421 ccttgttcca gacgttgcat cgcgctcttt aatacaccgc ctggaaagga tcatgcctgg
    30481 cccgcacagt tttcggcaga ttctttccac caatggacgc atgcctgagg ttcttctcgt
    30541 gcctgatatg aagctttttg ctggtaacgc caccccggta ctagcacaac gtattgccaa
    30601 ccgcctgtac acttcactcg gcgacgccgc tgtaggtcgc tttagcgacg gcgaagtcag
    30661 cgtacaaatt aacgaaaatg tacgcggtgg tgatattttc atcatccagt ccacttgtgc
    30721 ccctactaac gacaacctga tggaattagt cgttatggtt gatgccctgc gtcgtgcttc
    30781 cgcaggtcgt atcaccgctg ttatccccta ctttggctat gcgcgccagg accgtcgcgt
    30841 ccgttccgct cgtgtaccaa tcactgcgaa agtggttgca gacttcctct ccagcgtcgg
    30901 tgttgaccgt gtgctgacag tggatctgca cgctgaacag attcagggtt tcttcgacgt
    30961 tccggttgat aacgtatttg gtagcccgat cctgctggaa gacatgctgc agctgaatct
    31021 ggataaccca attgtggttt ctccggacat cggcggcgtt gtgcgtgccc gcgctatcgc
    31081 taagctgctg aacgataccg atatggcaat catcgacaaa cgtcgtccgc gtgcgaacgt
    31141 ttcccaggtg atgcatatca tcggtgacgt tgcaggtcgt gactgcgtac tggtcgatga
    31201 tatgatcgac actggcggta cgctgtgtaa agctgctgaa gcgctgaaag aacgtggtgc
    31261 taaacgtgta tttgcgtacg cgactcaccc gatcttctct ggcaacgcgg cgaacaacct
    31321 gcgtaactct gtaattgatg aagtcgttgt ctgcgatacc attccgctga gcgatgaaat
    31381 caaatcactg ccgaacgtgc gtactctgac cctgtcaggt atgctggccg aagcgattcg
    31441 tcgtatcagc aacgaagaat cgatctctgc catgttcgaa cactaatcga acccggctca
    31501 aagacccgct gcggcgggtt tttttgtctg taatatccat ttgtatgacc tatgcctcct
    31561 tcacctgcca tttagttgac agatgatgcg ctcacggatg aaacattatt gtgaacaaaa
    31621 tattttcctc acatgtgatg cctttccgcg ctctgatcga cgtttgctgg aaagaaaaat
    31681 atactgccgc acggtttacc cgtgacctga ttgccgggat aaccgtcggg attattgcta
    31741 tcccgctggc gatggcgttg gctattggta gtggtgtggc cccccagtac ggtttatata
    31801 ccgcagctgt tgcggggatt gtcattgctc taacgggtgg gtcacgcttt agcgtttccg
    31861 gtccgactgc ggcatttgtg gtaattctct atcccgtttc gcaacagttt ggactggcag
    31921 gactgctggt tgcgaccttg ttgtcgggaa tctttttgat tctgatgggg ctggcacgct
    31981 ttggtcgtct gattgagtat attccggttt ccgtcacctt aggtttcacc tcgggtatcg
    32041 ggatcaccat cggtaccatg cagattaaag attttctcgg tctgcaaatg gcccatgtcc
    32101 cggaacatta tctacaaaaa gtcggcgcat tatttatggc gctgccgacc attaatgtgg
    32161 gtgatgctgc cattggcatt gtgacgcttg gcattctggt tttctggccg cgtctgggca
    32221 ttcgtttacc cggtcacctt ccggccttgc tggctggttg cgcggtgatg gggattgtta
    32281 acctgctcgg cggacatgtt gctaccatcg gttcgcaatt ccactacgtc ctggccgatg
    32341 gttctcaggg taacggtatt ccgcaactgc tgccgcaact ggtgctgccg tgggatctgc
    32401 ctaattcaga attcacgcta acctgggatt ctattcgcac cctgctgcct gcggcattct
    32461 caatggcaat gctcggggcg atcgaatctc tgctctgcgc tgtggtactg gatggtatga
    32521 ccgggactaa gcacaaggcg aatagtgaac tggtcggtca ggggctgggg aatatcatcg
    32581 ctccattctt tggtggtatt accgctactg ctgccatcgc gcgttctgcc gctaacgtcc
    32641 gtgccggggc aacttcccct atctcggcgg tgatccactc tattctggtt attctcgccc
    32701 tgctggtact ggcaccactg ctctcctggc tgccgctttc cgccatggca gccctgctgt
    32761 tgatggtggc gtggaacatg agtgaagcgc acaaagtggt cgacttgctg cgtcatgcgc
    32821 cgaaagatga catcatcgtc atgctgctgt gtatgtcact gaccgtgctg tttgatatgg
    32881 ttattgccat cagcgtgggg atcgtgctgg catcgctgct gtttatgcgt cgtatcgcac
    32941 gtatgactcg cctggcaccg gtagtcgtag atgttccaga cgatgtcctg gttctgcgcg
    33001 ttattggccc gctgtttttt gctgctgctg aaggcttatt cacggacctg gagtcacgtc
    33061 ttgaaggcaa acggattgtg attctgaagt gggatgccgt tccggtactt gatgctggtg
    33121 gtcttgatgc gttccagcgt tttgtgaagc gtctgcccga aggatgtgaa ctgcgcgtgt
    33181 gcaacgtgga attccagcca ctgcgaacta tggctcgcgc aggcattcaa ccgatcccgg
    33241 gacgcctcgc gttcttcccg aatcgtcgcg cggcgatggc ggatttataa gtacgagatt
    33301 gaccagtcag cgcaaactga ctggtcagca aactgcatta tttgttagct atgacggcga
    33361 ttatcgctac ggcggcaacg tttgtcatag tgacgaaccc accagtaacg gtcgcacacc
    33421 tgttcatgcc cgccaacacg ggcacccgcc agccagagaa tggcaccgac gaaaatactt
    33481 agcactgcac catgtgcgaa atactggggc atattaaact gtggtaactg gttgaggatt
    33541 gaatacccca cgccgaccac cattaccacc agacccaacc ccatgagcac gttaccgagt
    33601 atcgaagcgt ttttgcgttt catatgtcac ctccggaact ttctgggttg taacagggaa
    33661 tacccctctt ccttatgtgt aaagtataga caacaccctg tgggttatgt gcgggcgtaa
    33721 tcacaattac aacccttatt tcaacaaaac tttacaaata aacgcctgaa caccttgact
    33781 ttctaatagt ccactcaggc aattattacg ttatctgcat ttgatacgca gttttttgtc
    33841 aagcggggcc gcaaccagta aactacgcgc cagttatgta cacactcagg acaaaaaaac
    33901 gtgacgatta aattgattgt cggcctggcg aaccccggtg ctgaatacgc cgcaacgcga
    33961 cataatgctg gtgcctggtt cgttgactta ctggcagagc gtttgcgcgc tccgctgcgc
    34021 gaagaggcta aattctttgg ttatacttcg cgagtcaccc ttggaagcga agatgtccgc
    34081 ctgttagtcc cgactacatt tatgaatctc agcggcaaag ccgttgcggc gatggccagt
    34141 tttttccgca ttaatccgga cgaaattctg gtggcccacg acgaactgga tctgcctcct
    34201 ggcgtcgcca aatttaagtt gggcggtggc catggtggtc acaatggact gaaagacatc
    34261 atcagtaaat tgggtaataa ccctaacttt caccgtttac gcatcggaat cggtcatccg
    34321 ggcgataaaa ataaagttgt cggttttgtg ttaggcaaac cgcctgttag tgaacagaag
    34381 ttaattgatg aagccatcga cgaagcggcg cgttgtactg aaatgtggtt tacagatggc
    34441 ttgaccaaag caacgaaccg attgcacgcc tttaaagcgc aataagtcgt tgtctgcggc
    34501 atttttgccg agtgccgtgt ataataggca aagttatttc catttctgca atctgttagc
    34561 aataacaggt tgattattaa gatattaagg tgatttaaat catgggattc aaatgcggta
    34621 tcgtcggttt gcccaacgtc gggaaatcta ccctgttcaa cgcgctgacc aaagccggta
    34681 ttgaagcggc caactttcca ttctgcacca ttgagccgaa cacaggcgtc gtaccaatgc
    34741 ctgaccctcg cctggatcaa ctggctgaaa tcgtaaaacc gcaacgtacg cttcccacga
    34801 ccatggaatt tgtcgatatc gccggtctgg taaaaggcgc atcgaaaggc gaaggtctgg
    34861 gtaaccagtt cctgaccaac atccgtgaaa ccgaagcgat cggtcacgtt gttcgctgct
    34921 ttgaaaatga caacatcatt cacgtttccg gcaaagttaa cccggctgac gatattgaag
    34981 ttatcaacac cgaactggcg ctggcagacc tcgacacctg cgaacgtgcg attcatcgcg
    35041 tacagaagaa agccaaaggt ggcgataaag acgcgaaagc tgagctggcg gtcctggaaa
    35101 aatgcctgcc ccagctggaa aacgcaggta tgctgcgcgc gctggattta agcgctgaag
    35161 agaaagcggc tattcgttac ctgagcttcc tgacgctgaa accaacaatg tacatagcca
    35221 acgtcaacga agatggtttt gaaaacaacc catatcttga ccaggtgcgt gaaatcgcgg
    35281 cgaaagaagg ttctgttgtg gttccggttt gtgctgctgt tgaagcagac attgccgaac
    35341 tggacgacga agaacgtgac gagtttatgc aggagcttgg gctggaagaa ccgggcctga
    35401 accgtgtgat ccgtgccggt tataagctgc tgaacctgca aacttacttc accgctgggg
    35461 tgaaagaagt gcgtgcatgg accattccgg ttggagcaac cgcgccgcag gcagcgggca
    35521 aaatccatac tgattttgaa aaaggcttta tccgtgcaca aaccatctcg tttgaagatt
    35581 tcattactta caaaggtgaa caaggcgcga aagaagcagg caaaatgcgt gcagaaggta
    35641 aagattacat cgtgaaagat ggcgatgtga tgaacttcct tttcaacgtc taattaattt
    35701 attgtctcat gaggtttcgc taaatctcat gaagagcgca aaagtatata aaatccacgc
    35761 aattgcgtgg atttttcatt tcaccgagtc ctaatcattc ttgtaacatc ctacactttt
    35821 gtgcttattg cctacagtga accacgagaa taattaaaga cataagtgtt cagatgactg
    35881 atatctcatt agcagtgata taacgtcagt ttttgttcca ctacacatca gccctcttca
    35941 cagctgatca atattgatct cgctccaata tgctggcgag aaaaggactt acctgaaggg
    36001 taatatcccg cagataaagt ccccgaataa cgtgattacg tgcgatcctg gcaagatgaa
    36061 aatgataacc agtagcacaa taataacgat aggccagaaa atccattgaa caaaaatgaa
    36121 tatcgatgtt ttttgctttt tcttattagt aatgcaaaaa tggcgcatat tgtttttgtt
    36181 atgcagatac atcaagcgaa aatcccataa ctaattagga ttattacaaa aaatcttcac
    36241 caaatttttt aaccgctgtt tgttgtaata tttttgccat attcatagta acgaattgga
    36301 ataagtaacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    36361 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    36421 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    36481 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    36541 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    36601 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    36661 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    36721 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    36781 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    36841 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    36901 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    36961 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    37021 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    37081 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    37141 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    37201 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    37261 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    37321 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    37381 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    37441 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    37501 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    37561 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    37621 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    37681 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    37741 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    37801 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    37861 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    37921 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    37981 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    38041 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    38101 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    38161 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    38221 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    38281 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    38341 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    38401 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    38461 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    38521 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    38581 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    38641 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    38701 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    38761 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    38821 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    38881 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    38941 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    39001 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    39061 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    39121 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    39181 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    39241 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    39301 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    39361 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    39421 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    39481 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    39541 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    39601 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    39661 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    39721 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    39781 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    39841 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    39901 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    39961 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    40021 aggtccgcgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    40081 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    40141 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    40201 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    40261 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    40321 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    40381 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    40441 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    40501 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    40561 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    40621 agcggatgtg ccagttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    40681 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    40741 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    40801 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    40861 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    40921 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    40981 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    41041 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    41101 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    41161 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    41221 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    41281 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    41341 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    41401 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    41461 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    41521 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    41581 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    41641 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    41701 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    41761 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    41821 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    41881 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    41941 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    42001 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    42061 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacaccg tattaattga
    42121 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gacgcctgtg cggaactggg
    42181 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    42241 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtaggcat
    42301 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    42361 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    42421 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    42481 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    42541 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    42601 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    42661 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    42721 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    42781 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    42841 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    42901 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    42961 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    43021 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    43081 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    43141 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    43201 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    43261 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    43321 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    43381 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    43441 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    43501 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    43561 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    43621 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    43681 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    43741 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    43801 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    43861 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    43921 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    43981 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    44041 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    44101 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    44161 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    44221 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    44281 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    44341 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    44401 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    44461 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    44521 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    44581 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    44641 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    44701 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    44761 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    44821 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    44881 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    44941 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    45001 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    45061 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    45121 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    45181 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    45241 attagggccg ctgtttaatg atgtgcaaaa cgtcaaactt tttccggacc aaaaaacctt
    45301 tgccgatgcc gtgcctaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    45361 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    45421 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    45481 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    45541 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acaattactt
    45601 caccatgtta ggacttgccg aaagtggtca ctgggataaa atcgcggata tggtggccaa
    45661 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    45721 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    45781 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    45841 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatag
    45901 tacccttctc aaccgctacc gggacgatcg cgatacgcca cgaccagagt catgggtgga
    45961 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    46021 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    46081 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    46141 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    46201 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    46261 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    46321 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    46381 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    46441 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    46501 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    46561 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    46621 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    46681 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    46741 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    46801 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    46861 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    46921 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    46981 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    47041 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    47101 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    47161 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    47221 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    47281 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    47341 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    47401 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    47461 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    47521 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    47581 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccgactt ttggcgagag
    47641 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    47701 atg
//
LOCUS       DAJVPT010000017        46719 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVPT010000017 DAJVPT010000000
VERSION     DAJVPT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(655..1431)
                     /gene="sseB"
                     /locus_tag="OQM47_000961"
     CDS             complement(655..1431)
                     /gene="sseB"
                     /locus_tag="OQM47_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1479211.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1573..2856)
                     /gene="pepB"
                     /locus_tag="OQM47_000962"
     CDS             complement(1573..2856)
                     /gene="pepB"
                     /locus_tag="OQM47_000962"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1479212.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2915..3115)
                     /gene="iscX"
                     /locus_tag="OQM47_000963"
     CDS             complement(2915..3115)
                     /gene="iscX"
                     /locus_tag="OQM47_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1479213.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3127..3462)
                     /gene="fdx"
                     /locus_tag="OQM47_000964"
     CDS             complement(3127..3462)
                     /gene="fdx"
                     /locus_tag="OQM47_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1479214.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3464..5314)
                     /gene="hscA"
                     /locus_tag="OQM47_000965"
     CDS             complement(3464..5314)
                     /gene="hscA"
                     /locus_tag="OQM47_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1479215.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5331..5846)
                     /gene="hscB"
                     /locus_tag="OQM47_000966"
     CDS             complement(5331..5846)
                     /gene="hscB"
                     /locus_tag="OQM47_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1479216.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5942..6265)
                     /gene="iscA"
                     /locus_tag="OQM47_000967"
     CDS             complement(5942..6265)
                     /gene="iscA"
                     /locus_tag="OQM47_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1479217.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6282..6668)
                     /gene="iscU"
                     /locus_tag="OQM47_000968"
     CDS             complement(6282..6668)
                     /gene="iscU"
                     /locus_tag="OQM47_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1479218.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6696..7910)
                     /gene="iscS"
                     /locus_tag="OQM47_000969"
     CDS             complement(6696..7910)
                     /gene="iscS"
                     /locus_tag="OQM47_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1479219.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8022..8510)
                     /gene="iscR"
                     /locus_tag="OQM47_000970"
     CDS             complement(8022..8510)
                     /gene="iscR"
                     /locus_tag="OQM47_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1479220.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8780..9520)
                     /gene="trmJ"
                     /locus_tag="OQM47_000971"
     CDS             complement(8780..9520)
                     /gene="trmJ"
                     /locus_tag="OQM47_000971"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1479221.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9639..10442
                     /gene="suhB"
                     /locus_tag="OQM47_000972"
     CDS             9639..10442
                     /gene="suhB"
                     /locus_tag="OQM47_000972"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1479222.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10587..11441
                     /locus_tag="OQM47_000973"
     CDS             10587..11441
                     /locus_tag="OQM47_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1479223.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11632..12912
                     /gene="csiE"
                     /locus_tag="OQM47_000974"
     CDS             11632..12912
                     /gene="csiE"
                     /locus_tag="OQM47_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1479224.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12904..14043)
                     /locus_tag="OQM47_000975"
     CDS             complement(12904..14043)
                     /locus_tag="OQM47_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1479225.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14203..15093)
                     /gene="hcaR"
                     /locus_tag="OQM47_000976"
     CDS             complement(14203..15093)
                     /gene="hcaR"
                     /locus_tag="OQM47_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1479226.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15229..16590
                     /locus_tag="OQM47_000977"
     CDS             15229..16590
                     /locus_tag="OQM47_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1479227.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16587..17105
                     /locus_tag="OQM47_000978"
     CDS             16587..17105
                     /locus_tag="OQM47_000978"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1479228.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17105..17425
                     /locus_tag="OQM47_000979"
     CDS             17105..17425
                     /locus_tag="OQM47_000979"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1479229.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17422..18234
                     /gene="hcaB"
                     /locus_tag="OQM47_000980"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17422..18234
                     /gene="hcaB"
                     /locus_tag="OQM47_000980"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1479230.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETPIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18244..19461
                     /locus_tag="OQM47_000981"
     CDS             18244..19461
                     /locus_tag="OQM47_000981"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1479231.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            19558..19980
                     /locus_tag="OQM47_000982"
     CDS             19558..19980
                     /locus_tag="OQM47_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1479232.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20028..20900)
                     /locus_tag="OQM47_000983"
     CDS             complement(20028..20900)
                     /locus_tag="OQM47_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1479233.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20912..22006)
                     /locus_tag="OQM47_000984"
     CDS             complement(20912..22006)
                     /locus_tag="OQM47_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1479234.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22039..23037)
                     /locus_tag="OQM47_000985"
     CDS             complement(22039..23037)
                     /locus_tag="OQM47_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1479235.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23062..24573)
                     /locus_tag="OQM47_000986"
     CDS             complement(23062..24573)
                     /locus_tag="OQM47_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1479236.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24589..25583)
                     /locus_tag="OQM47_000987"
                     /pseudo
     CDS             complement(24589..25583)
                     /locus_tag="OQM47_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25680..28781)
                     /locus_tag="OQM47_000988"
     CDS             complement(25680..28781)
                     /locus_tag="OQM47_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1479237.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(28788..28961)
                     /locus_tag="OQM47_000989"
     CDS             complement(28788..28961)
                     /locus_tag="OQM47_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479238.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29079..30272
                     /locus_tag="OQM47_000990"
     CDS             29079..30272
                     /locus_tag="OQM47_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1479239.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30470..31723)
                     /gene="glyA"
                     /locus_tag="OQM47_000991"
     CDS             complement(30470..31723)
                     /gene="glyA"
                     /locus_tag="OQM47_000991"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1479240.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32050..33240
                     /gene="hmpA"
                     /locus_tag="OQM47_000992"
     CDS             32050..33240
                     /gene="hmpA"
                     /locus_tag="OQM47_000992"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1479241.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33285..33623)
                     /gene="glnB"
                     /locus_tag="OQM47_000993"
     CDS             complement(33285..33623)
                     /gene="glnB"
                     /locus_tag="OQM47_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1479242.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33684..35018)
                     /gene="glrR"
                     /locus_tag="OQM47_000994"
     CDS             complement(33684..35018)
                     /gene="glrR"
                     /locus_tag="OQM47_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1479243.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35008..35721)
                     /gene="qseG"
                     /locus_tag="OQM47_000995"
     CDS             complement(35008..35721)
                     /gene="qseG"
                     /locus_tag="OQM47_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1479244.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35886..37313)
                     /gene="qseE"
                     /locus_tag="OQM47_000996"
     CDS             complement(35886..37313)
                     /gene="qseE"
                     /locus_tag="OQM47_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1479245.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37871..41758)
                     /gene="purL"
                     /locus_tag="OQM47_000997"
                     /gene_synonym="purI"
     CDS             complement(37871..41758)
                     /gene="purL"
                     /locus_tag="OQM47_000997"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1479246.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42017..43573
                     /gene="mltF"
                     /locus_tag="OQM47_000998"
     CDS             42017..43573
                     /gene="mltF"
                     /locus_tag="OQM47_000998"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1479247.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43570..44106)
                     /gene="tadA"
                     /locus_tag="OQM47_000999"
     CDS             complement(43570..44106)
                     /gene="tadA"
                     /locus_tag="OQM47_000999"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1479248.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44131..44766)
                     /gene="yfhb"
                     /locus_tag="OQM47_001000"
     CDS             complement(44131..44766)
                     /gene="yfhb"
                     /locus_tag="OQM47_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1479249.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44975..45823
                     /locus_tag="OQM47_001001"
     CDS             44975..45823
                     /locus_tag="OQM47_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1479250.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46162..46716)
                     /gene="pinE"
                     /locus_tag="OQM47_001002"
                     /pseudo
     CDS             complement(46162..46716)
                     /gene="pinE"
                     /locus_tag="OQM47_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11130 a        12832 c        11728 g        11029 t
ORIGIN      
        1 attattgttt atttagcgta ttatcgacac cggccctttc cgccgtgttc ggtaataaaa
       61 taacctggct tattagtccg aattcagaca aatataaaca aatcctgctc aaaattaaaa
      121 attctaaccg gtaaaagata ttacttaaac atgtaaattt actttccttt aaaaaacaaa
      181 aaaccgccaa aatcaggcgg ttatttgttg ctggtccggt tcgcggcctt tccagcaggt
      241 tgtattaccg tagtaatgca agcgcgtctc agcggagaca atactcgcca gtaactctct
      301 ttttgtcaag caaaagagag ttattattgt tctgttagtg tattatccac tgcggccctt
      361 tccgccgtct cgcaagcggg cgctggctta aggaaaggat gttccgcgac cgttagagca
      421 agcgcgtctc agcgcttgct atcgcggcaa tatcgccagt ggtgctgtcg tgatgcggtc
      481 ttcgcatgga ccgcacaatg aagatacggt gcttctgtat cgtacttatt gtttctggtg
      541 cgctgttttc ccaagtaaat aataaccgga gtctctccgg cgacaattta ctggtggtta
      601 acaaccttca gagcagcaag taagcccgaa tgccgccctt tagggcggca tattttagat
      661 tatccggttt tgtttaaagt cacgcaaaaa accaccccag cgacgttcat agaatggcgc
      721 aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct gacaaatatc
      781 gattggttca tcgccaggta atgtatcggt cgctacactt cccgtcgcct gaataatttc
      841 ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct cttcgttctc
      901 tttaattgaa caaataaaag cacgcttcac cggcttaatg gttttaaata aggtggtgag
      961 tgaatcaatc atttgtgctg gcggctctgc gacttccgat aatatcagcg attcaccgcc
     1021 ttccaggatt tcctggctgc tcagcggatt tccctcttca ccaatcaaca aactgatttc
     1081 acgcggcata aattctttac cggtcggcag tttggcatta aggaagagcg tttcgccaag
     1141 ggtcatctca aacaatgtgc gaacgggcat tacgacaaat gcctgttcgt cttcaaccgc
     1201 ctgttgaagc gcttctaacg aggtgaaaaa aggaatgacg ctggtgccgt cttctttttc
     1261 ccagtgctgt aaatcaagcg cactatcttc aaccacagcc tcgccctgcg ccgccgtacc
     1321 aggcacccag acggtggatt ccagtagagt acggaaaaag gccgggcggt gcgccggttc
     1381 ggttgctgct ttttccagca ggtcttcaag ttcgtttttt gtttcggaca taagaaccac
     1441 aattcattca acgttcgggg cgcaaatgcc tgatgcgcta cgcttatcag gcctacaagg
     1501 gattcgcaat ttgttgaatt tgcagaattt gtaggccgga taaggcgttt acgccgcatc
     1561 cggcatatta gtttacgccg ttaacagatt agctatcgtg cgcacaccaa gtcccgtagc
     1621 gcccgcagac cactgttcaa ccggcgcttt acggtaagtc gccgagcagt cgatatgcag
     1681 ccagccttgc tgatagttct caacaaagtg cgacaggaag cccgccgccg tgctcgcgcc
     1741 tgccggatac gccgcgcttc cggtattgtt cagttcggca aagttagacg gcagctggct
     1801 gcggtggaat tccgccagcg gcagacgcca gaatggttcg ttctcttgtg aggcactcgc
     1861 cagtaaacga ccggcaagcg catcgtcaaa actgaacagc gcgtgataat cattacccag
     1921 cgcagttttc gccgccccgg tgagggtcgc cgcatcaatg atcatttccg gtttctgcgc
     1981 actggcatca atcagaccat cggcaagcac cagacgccct tcggcatcag tgttcatcac
     2041 ttcaactttt ttaccgttgc gataggtgat gatatcgccc agcttgaacg cattgccgct
     2101 aatcaggtta tccgcacagc agaggaacag cttcacgcgc ttgttcagtc cgcgcataat
     2161 ggcaaatgcc agcgccccgg taaccgttgc cgcgccgccc atgtccgact tcatcgagtc
     2221 cataaacgca gtctgtttga tgctgtagcc gccggagtca aaagtgatac ctttacctac
     2281 caggcacgcg tacaccggtg cttctttatc gccagttggg ttgtaatcca gcgccagcaa
     2341 taccggagaa cgttctgaac cgcgcccgac agtgtgcagc cccatataac cttgctcacg
     2401 cagatcttcg cctttggtga tccgataagt cacacgatcg cccgcgacgt tgctgatcag
     2461 atcaacagca cgctgtgcca gttgcgatgg tcccaattct tctgccggtg cgttgatggt
     2521 gtcacgcacc cagtcgatga tcatcaggcg gttatccagt tcctggcgct gggcatcgtc
     2581 cagatccggc cactccactt tacgcgtgcc tttcgggcct ttataacctt gccagaatgc
     2641 ccagcagcga tccgcatccc agccttcacc gcttaactga acatgcttga tgcccagacc
     2701 gtcaatcttg cgggccgcac gctggatcag ccccagatcg tctgccccgt tcaaatgcag
     2761 ggtaatgccg tcattattaa tgctgtaagt tgctttttct ccccagcgcg cgtcggcagg
     2821 ttgggtagag agggtaatct tcatcgcttc tgtcatttta gttatcctta ttcgtttaac
     2881 agcaaaacgg gctaccccca ggcagcccgt tcattcactc ggcttcgtcc agccagacta
     2941 acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca
     3001 gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat
     3061 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt cccatactaa
     3121 cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc
     3181 ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt
     3241 gtccagcatg tcgtcttcct gctctgagct ttccggcagt gagtcaaaac cttcacgaac
     3301 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcaatacc
     3361 gttacgcagc gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc
     3421 agggcagagg tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
     3481 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
     3541 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg ccctgcgcca
     3601 cttcactcag gtgagcggca gcattgtcaa tgacctgacg ctctgcggcg cttaacagcg
     3661 cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt
     3721 gttctgccag catacgggct tttacgtcct gctcggcata gctcattgag tctttgatca
     3781 tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa
     3841 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
     3901 tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc gcaaaacgcg
     3961 ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg
     4021 ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga atagtggtat
     4081 tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga
     4141 tcacatcaag cagcagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa
     4201 tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt ggacgaccga
     4261 aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
     4321 cttccagcac ttcgtcagct tctacacccg cgtctttcag cgcgcgacga caagccagta
     4381 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
     4441 gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa
     4501 tggtggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga atgtccgcct
     4561 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac
     4621 cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg
     4681 tcccgccacc gaggtcataa acagcgatca cgccttcctg accggaatcc agcccgtagg
     4741 cgatagccgc agcagtcggt tcgttaagta agcgcagaac atgcaacccc gccagacgcg
     4801 ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta
     4861 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
     4921 ccgcagaaac gcgcaccggg ttcagtagcc ccgccgccgt ttcaatcatc ggcaggccgt
     4981 tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
     5041 agcgccccat caggcgttta acagaactaa tggtgttggc ggtgtcaagc gctgcgttag
     5101 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca
     5161 ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca
     5221 gcgagttggt tgtgcccagg tcaataccag ccgccagacg acgctgatgc ggcgcagcac
     5281 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
     5341 agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa acgcagctta
     5401 cgcaccgtat ccgccgccgc gtcccacgcc tcgttgtcta actgttcaac catcaactga
     5461 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
     5521 ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat caggaacgcg
     5581 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat
     5641 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt tgcagattgc
     5701 tgtacggcgg cgagttgttc cgccaggctt ccgctggcga atttatcagg atgatactga
     5761 cgttgtagat cctgaaaacg caggctcagt gcctgggtat cgagttgata acgggcaggc
     5821 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
     5881 gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
     5941 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg ttggtgaatt
     6001 taaacccttc attcaggcct tcttttacga agtccagctg cgtaccgtcc agaaattgca
     6061 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
     6121 tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc
     6181 ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact cgcgctgctg
     6241 cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
     6301 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
     6361 ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg
     6421 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcggaaccg
     6481 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
     6541 aacttcatta cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg
     6601 ttgttatcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
     6661 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
     6721 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
     6781 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
     6841 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
     6901 taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc gaggtctttc
     6961 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
     7021 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
     7081 agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
     7141 cgataggctt cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg
     7201 cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cacgcggttt acgacgcaca
     7261 tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
     7321 actttcaact ggctcaggtc gattggcagt ttgcccacgc tctgggttgc atcaacgtga
     7381 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
     7441 atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgccgcttcg
     7501 agttctttca ggtcgataat accgttacgc tgcggtgcca ggtaggtgac ttcgaaacct
     7561 tcgcgctcca gctgacggca agtatccagt accgctttgt gttcggtttt gctggtgatg
     7621 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat
     7681 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
     7741 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgagag
     7801 gccgggttac caaaggttcc gtccatcgtc atgaactgca tcattttctc ggcaacacgc
     7861 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
     7921 actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg accgtgttta
     7981 cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg taacttaacg
     8041 tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca
     8101 tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg
     8161 agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag
     8221 ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg
     8281 ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg
     8341 ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa
     8401 attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca
     8461 acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc
     8521 acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag
     8581 ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg
     8641 tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc ggatgcggtg
     8701 tgaacacctt atccgaccta cgattctgta cattgtaggc ctgataagac gcgttaagcg
     8761 tcgcatcagg caaccactgt tattcggcct tgttaccttt attctgctgc tcaatagaag
     8821 ccagaatccc gcgcaggata ttcaattcct ggctttccgg gcgcgcacgg gtaaacagac
     8881 ggcgcaattt attcatcacc tgccccggat ggttttcacg gataaaaccg gttgccagca
     8941 gcgtttgttc cagatgacca taaaaacgct ccagatcatc gaccagcgga tacggcgtct
     9001 cttcatgctc gacctgctcg ccattttcct gagttgccag ccaggccata cgcacttcat
     9061 aggcaataac ctgaaccgcc attgccaggt tcagcgaact gtattccggg tttgccgcaa
     9121 tcgcgacatg ataatggcat ttctgcaact cttcattggt caagccgacg cgctcacgac
     9181 caaacaccag cgccaccggg gtatttgccg cttcagcgac gcttttcaga ccgcattcgc
     9241 gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc gctggtgccc accaccaggc
     9301 tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg agcattacca atcacatcgc
     9361 tggcccctgc agccagggca atcgcctggg agtcgggttt caccagtgga ttaaccagcc
     9421 acagattggt taatcccatt gttttcatgg cacgggcaac agaacccata ttgccggtgt
     9481 gtgacgtctc caccagcaca attcgaatat tttgcagcat tgtctttctt catctaaaga
     9541 ttattcacgc atcttatcat aaaacgaaga cagatgccga tctcgctgct atactctgcg
     9601 ccgttttccc gttctttaac atccagtgag agagaccgat gcatccgatg ctgaacatcg
     9661 ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa aaactatgaa accccggacg
     9721 ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac caacgtagat aaagctgccg
     9781 aagcggtgat tatcgacacg attcgtaaat cttacccaca gcacaccatc atcaccgaag
     9841 aaagcggtga acttgaaggt actgatcagg atgttcaatg ggttatcgat ccactggatg
     9901 gtactaccaa ctttatcaaa cgtctgccgc acttcgcggt atctatcgcc gttcgtatca
     9961 aaggccgcac cgaagttgct gtggtatacg atcctatgcg taacgaactg ttcaccgcca
    10021 ctcgcggtca aggcgcacag ctgaacggct accgtctgcg cggtagcacc gctcgcgatc
    10081 tcgacggtac cattctggcg accggcttcc cgttcaaagc aaaacagtac gccactacct
    10141 acatcaacat cgtcggcaag ctgttcaacg aatgtgcaga cttccgtcgt accggttctg
    10201 cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga cggtttcttt gaaatcggtc
    10261 tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg tgaagcgggc ggcatcgtca
    10321 gcgacttcac cggtggtcat aactatatgc tgaccggtaa catcgttgct ggtaacccgc
    10381 gtgttgtgaa agccatactg gcgaacatgc gtgacgagtt aagcgacgct ctgaagcatt
    10441 aatgactcag gcgggtgata tcactcaccc gccctcgcct ttcaggcgct attccgaaat
    10501 acttcctcac cgctttactt tctttcgtca ctctcccacc atctttcccc gattaatgga
    10561 tgataaagaa gtcgcaagga aaggttatgg cactgccagt gaacaaacgc gttcccaaaa
    10621 ttctgtttat tctctttgtt gttgccttct gcgtttattt agtgccgcgc gttgccatca
    10681 acttcttcta ttatcctgac gataaaattt acggtcccga cccctggtcg gcggaatccg
    10741 tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg gtttattcct tcttcgacgg
    10801 gccctgctga caacgccatc gcaaccatca ttcatgctca cggcaatgcc ggaaatatgt
    10861 ccgcccactg gccgctggtc agttggttac ctgagcgtaa tttcaacgtt tttatgtttg
    10921 attatcgcgg gtttggtaaa tcaaaaggca ctccgtccca ggccggattg ctggacgata
    10981 cgcaaagcgc catcaatgtg gtgcgccatc gcagtgatgt aaacccacaa cagctggtgc
    11041 tgttcgggca gagtattggc ggggcgaata ttcttgctgt tattggtcag ggtgatcgtg
    11101 aaggcatacg tgcggtgatc ctcgactcca catttgcctc ttatgcaacc atcgccaacc
    11161 agatgatccc cggcagtggc tacttacttg atgagagtta cagcggcgaa aattacatcg
    11221 ccagcgtcag cccgatcccg cttttactca ttcacggtaa agccgatcac gttatcccat
    11281 ggcagcacag cgaaaagttg tatagcctgg caaaagagcc aaaacggttg atcctaatcc
    11341 cggatggcga acacattgat gctttttccg atcgtcacgg caatgtttat cgcgaacaga
    11401 tggtggactt tatccttagc gcgttgaatc cgcagaacta acccatgatc gctagcacga
    11461 taatcattca caaaaccacc ttaagacatg ctaatccact ggtcagaaca gtttaaaatg
    11521 agaaaaattc tgtgacgctt gccaacattt ctgatgatta gcattccctt cgccatttcc
    11581 ctgagcaaac tttagctatt cttatcaatt atgcttatgg gagatctaca gatgatgcct
    11641 acgcttgctc caccatctgt cctttcggct ccccagcgcc gctgccagat cttgctgacg
    11701 ctctttcagc cggggttgac cgccaccatg gcaaccttca gcgagcttaa tggtgtggat
    11761 gatgatattg ccagtcttga tatcagcgaa acaggacggg agatcctgcg ctatcatcaa
    11821 ctcacactga cgactggtta tgacggtagc taccgggttg aaggtacagt gcttaaccaa
    11881 cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc tgtgtccgtc atttattacc
    11941 agccaattca cccccgccct gaagagtgaa ctgaagcggc gcggaattgc gcgtaacttt
    12001 tacgacgata ccaatctaca agcgttagtg aatctctgct cccgacggct gcaaaaacgc
    12061 tttgaatcgc gcgatattca tttcctgtgt ctgtatctgc aatattgttt gctgcagcac
    12121 cacgctggaa ttacgcctca gtttaatccg ctccaacgtc gctgggcgga atcctgcctt
    12181 gaatttcagg tagcgcagga aattggacgc cactggcagc gtcgggcgct ccagcatgta
    12241 ccgcctgatg agccactgtt tatggcatta cttttttcca tgttgcgggt tcccgatcca
    12301 ctgcgggatg cgcatcagcg ggacagacaa ttgcgtcagt ctatcaaacg tctggtaaac
    12361 cattttcgtg agctgggaaa tgttcgtttt tatgatgaac aggggttatg tgatcagctt
    12421 tatatccacc tcgcccaggc gttaaatcgc agtttgtttg ccatcggtat tgataatacc
    12481 ctgccggaag agttcgccag actgtatcca cgcctggtac gcaccacccg cgcggcgctg
    12541 gccggatttg aaagtgaata cggcgtccat ctttctgatg aggaaagtgg tctggtcgcg
    12601 gtgattttcg gtgcctggct aatgcaggaa aacgatctgc atgaaaaaca gattattcta
    12661 ctgaccggga atgatagcga gcgagaagcg cagattgagc agcagttacg cgaactaacg
    12721 ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat ttttgcagac aggcgctccg
    12781 cgtggcgcgg cactgattat tgcgccttat accatgccgt taccgctctt ttcaccaccg
    12841 ctgatctata cggacctgac gctgacaaca catcaacagg agcagatccg caaaatgctc
    12901 gaatcagcat gagggaacaa ctttcgggcg caaaaacatt gccggaagcg ccaccagcgc
    12961 cattacccag aacacgccgt ggcccagata ttgatacagg aaaccggcga aaacggtcat
    13021 gatagcgata ctgccgccca tcgcgacggc agagtaaacc gcctgtaaac ggatgacttc
    13081 gctaacctgg cgagcagcaa tataacgcat ggcggccagg tggcagaccg tgaaggtgcc
    13141 gcaatgcaga atttgcacca ctatcaacca cggcaacgcc gtagttgctc ccataatgcc
    13201 ccagcgcact acgccgcaaa tcgccgagat caacagcata tcgcgagcac tacaacggcg
    13261 gaaaagttta ttactcagcg caaagataat gacttccgcc accacgccca gcgaccacaa
    13321 ataccccacc gccgaggccg agtagccagc tgcctgccag taaatggcgc taaaaccgta
    13381 ataggccgca tgtgccccct gcaataaaca aacgcaggcc agaaagcgcc agttctggcg
    13441 aaccagcgcc aaccacgcag accaaccggt gctctcctgc tggcggcttg ccccttgtgg
    13501 ctgaatcgtc ggacggatga gaaagccgag cagcatggat gccacgccca acgtcaacag
    13561 cacgaggatc acccgataat caaacatagt gaccagtttg cccgtcagcg ccgagccaat
    13621 gacaaacgcc accgagcccc acagtcgcac tttgccgtaa tcaagcggga actgcttttg
    13681 ccacgtattc gccagtgcat cggtcaacgg taccagcggt gagaaaaaga ggttaaagcc
    13741 aatcatcacc agcatcagcc acgctacgtg cgcccccgcc cagaaggcga cagcaaagag
    13801 aagtggcagc agtgccagca cgcgcaaggc ggaaatcagg cgggaaggat cgctgacgcg
    13861 gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga cctgccccca ataacaggcc
    13921 gatggtttct ggcgttaaac caatcccttt aagccagacg ctccagaaag gtaaaaaaat
    13981 gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac cagcgcgtgg attgcaaaac
    14041 catgagtccc tcccgtcagg aggcgttagt ctggggttaa tcgaatgttg gtgcaagttg
    14101 aaaaataaga atattcgagc ctgcttcccg atgtctgtaa cgccagttca caagcactaa
    14161 aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt aattatgccg ttacgcttgc
    14221 caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc agcttcatct cccctttctt
    14281 ccacgccatc agtaaagcaa tggaaggcac atttccggca atgggacgaa aaacaacctg
    14341 cccggtatta aaattattca tataaccggg tatcaaagtg acgcccagcc ccatgcccac
    14401 cagattcatg gtcaccagaa tattcgttgc cacctggacg atatttggct ggctgttttc
    14461 ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg gaatacgccg gatcggtact
    14521 gacgaaattc acgccatcca gttgcgcggc ggtgatctct ttttcatgtg ctaaagggtg
    14581 atcaaccggt aacaccacca ctaatggttc gtcaaaaagc tccagataat caatctccgg
    14641 gctataaaca ggatggcgca ttaagccgac gtcgagttca ccacggcgaa ttttttcctc
    14701 ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg tctggctgtc tgagacgaaa
    14761 catcggtaat acttttggca ataaattcac ttccgccgat ggcacaaagc caatggttaa
    14821 ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg gcattttccg cttgttcgag
    14881 gattgccagc gcgtcctgga gaaaacattc tcccgccgcc gtcaacgcga ctttgcgctt
    14941 atccctcacc agtaacggaa caccgacaca gttttcaaga tcgcggatct ggctgcttaa
    15001 cgaaggctgt gaggtatgca gtttttccgc cgcacgggta aagttcagtg cctgcgccac
    15061 tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc cccttgttat cgaaaaaacg
    15121 tctcagctgg tagaaattaa ctatttcaca tattagcaac caaccagcaa catccttatg
    15181 gcacaaaaat agaaggtcaa tacatcttat ctttcaggat taaaaaatat gaccacaccc
    15241 tcagatttga acatttacca actgattgac acccaaaacg gtcgggtcac tccgcgtatt
    15301 tataccgacc cggacattta ccaactggag cttgagcgta ttttcggtcg ttgctggcta
    15361 tttctcgccc acgaaagcca gatcccaaaa cccggtgatt tctttaacac ctacatggga
    15421 gaagatgcag ttgtcgtggt gcgccagaaa gacggcagca tcaaggcgtt tctcaaccaa
    15481 tgccgccacc gggccatgcg tgtgagttat gcagattgtg gcaacagtcg cgcctttacc
    15541 tgcccgtatc acggctggtc ttatggcatt aacggcgagt tgatcgatgt accgctggaa
    15601 cctcgcgcct acccacaagg gttgtgtaaa tcccactggg ggctaaacga agttccttgt
    15661 gtggagagtt ataaaggact gatttttggc aactgggata ccagcgcacc gggcctgcat
    15721 gattacctgg gtgacattgc ctggtatctg gatggcatgc tggatcgtcg cgaaggcggc
    15781 accgaaattg tcggcggcgt acaaaagtgg gtgatcaact gtaactggaa attcccggca
    15841 gagcagttcg ccagtgacca gtatcatgct ctgttcagcc atgcttctgc cgttcaggta
    15901 ttaggggcga aagatgatgg cagcgataag cgcctcggtg atggacaaac cgcccgcccg
    15961 gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg acggtcacgg tagcggtttc
    16021 ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg gcgcagtttc aagctattac
    16081 cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag ttcgcgccct gcgcctggcg
    16141 ggtcataaca atatattccc cacgctttca tggctaaacg gcactgccac actccgcgtc
    16201 tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt tctgtattac tgacaaagcc
    16261 gcctccgatg aagttaaagc cgcttttgaa aacagcgcca ctcgtgcttt tggtcctgct
    16321 ggttttctcg agcaggatga ctcggagaac tggtgtgaaa tccagaaatt gcttaaaggc
    16381 caccgcgccc gcaacagcaa actgtgtctg gaaatggggc ttggtcagga aaagcgccgc
    16441 gacgacggca ttcctggcat tactaactat atcttttcag aaactgccgc tcgtggaatg
    16501 taccaacgct gggccgatct tctgagtagc gaaagctggc aggaagtgct cgataaaacc
    16561 gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt ttcactggag ttacatcacc
    16621 gcattagcca gtttctcttt cacgaagcca gtctgctgga tgactggaaa tttcgtgact
    16681 ggttggcaca gctcgacgaa gagattcgtt acaccatgcg caccacggtt aacgcgcaaa
    16741 cacgcgaccg tcgcaaaggc gtccagccgc cgacgacctg gattttcaat gacaccaaag
    16801 accagctgga gcggcgaatc gcccgtctgg aaacgggcat ggcctgggca gaagagccgc
    16861 cgtcacgcac tcgtcactta atcagcaact gccaggtaag cgaaaccgac atcccaaacg
    16921 tatttgccgt gagggtgaat tatctacttt atcgggcaca aaaagagcgc gatgaaacat
    16981 tctatgttgg aacgcgtttc gacaaagttc gccgtctgga agatgacaac tggcgcttgc
    17041 tggaacggga tatcgtcctg gatcaagcgg tgatcacttc ccataacctg agtgtactgt
    17101 tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg ccggagggtg aggctctccg
    17161 gatagatacc tcgcccgtca tcgccttgtt caacgtgggc ggcgagtttt atgccattaa
    17221 cgatcgttgc agccatggta atgcgtcgat gtcagaaggg tatctggaag atgacgccac
    17281 ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa acggggaaag cgttatgcct
    17341 gcccgccacc gatccgctca ccacttatcc agtacacgtt gaaggtggtg acattttcat
    17401 cgacttaccg gaggcgcagc catgagcgat ctgcataacg agtccatttt tattaccggc
    17461 ggcggatcgg gattagggct ggcgctggtc gagcgattta tcgaagaagg cgcgcaagtt
    17521 gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc gtcagcgatt tggcgaacat
    17581 attctggcgg tggaaggtaa cgtgacctgt tatgccgatt atcaacgcgc ggtcgatcag
    17641 atcctgactc gttccggcaa gctggattgt tttatcggca atgcaggcat ctgggatcac
    17701 aatgcctcac tggttaatac tcccgcagag acgctcgaaa ccggcttcca cgagctgttt
    17761 aacgtcaacg tactcggtta cctgctgggc gcaaaagcct gcgctccggc gttaatcgcc
    17821 agtgaaggca gcatgatttt cacactgtca aatgccgcct ggtatcctgg cggcggtggc
    17881 ccgctgtaca ccaccagtaa acatgccgca accggactta ttcgccaact ggcttatgaa
    17941 ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg gtatggccag cgacctgcgc
    18001 ggcccacagg cgctcgggca aagtgaaacc ccgataatgc agtctctgac gccggagaaa
    18061 attgccgcca ttttaccgct gcaatttttc ccgcaaccgg cggattttac ggggccgtat
    18121 gtgatgttgg catcgcggcg caataatcgc gcattaagcg gtgtgatgat caacgctgat
    18181 gcgggtttag cgattcgcgg cattcgccac gtagcggctg ggctggatct ttaaggaagc
    18241 acgatgaaag aaaaaacgat cattattgtc ggtggcgggc aagcggcggc aatggctgcg
    18301 gcctcgctac gccagcaagg gttcaccggt gagctgcatc tgttttccga tgagcgacat
    18361 cttccttatg aacgacctcc actctcgaaa tccatgttgc tggaagattc cccgcagtta
    18421 cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg ttcatctgca ttccggtgta
    18481 accatcaaaa cgctgggtcg cgacacacga gagttagtgt taaccaacgg cgaaagctgg
    18541 cactgggatc agctttttat agcaaccggc gcggcagctc gaccgctgcc gttgcttgat
    18601 gcactgggag aacgctgctt taccctacac catgccggtg atgccgccag actgcgagaa
    18661 gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg gaactattgg tctggaactg
    18721 gctgccagcg ccacgcagcg cagcgcagcg cagcgcagat gtaaggtgac agtgattgaa
    18781 ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta tcttttacag
    18841 cgccatcagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca tgtggtcgat
    18901 ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga tgtggtgatt
    18961 tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct tgatactgcc
    19021 aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt tgccggtggc
    19081 gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag ctgggaaaac
    19141 gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct accgctactg
    19201 ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat tggcgatatg
    19261 cgtggcgatg actggctttg tcgtggcaac ccggaaactc agaaggcgat ttggtttaat
    19321 ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc agggccgtga gattcgccca
    19381 attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat agatgagaac
    19441 atcgcgctta aatcactgta atcaggataa ttagcgaata tctcaatgcc tggggcgtgg
    19501 cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg attggttatg
    19561 aacacactac gttattttga ttttggagct gcccgccccg ttttgttatt aattgcccgt
    19621 atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg ctttgacggt
    19681 acggtccaat atatggcctc gttgggcgcg ccaatgccga tgctggcagc gattattgcg
    19741 gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac ccgtccgctg
    19801 gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca ttactgggat
    19861 atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt cagtatcgct
    19921 ggcgcgttct tgctattggc aattaccgga ccgggggcaa tttctctcga tcggcgttag
    19981 gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt ttataaatta cagccactct
    20041 acccgcaacg acatctctga ggttgttgat tcccctggtg ctagcgcaat gagatcaccc
    20101 ccttccgggc gatgatgctc atccggcgca tggctcatcg gttccagaca gaagaaatca
    20161 aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga tgaaataaca cggtgcaggt
    20221 ggcgtcgttt ccatgatgat ggcataacct tcctgcggct gctcgatgcg ggcctgacca
    20281 ttccatccgg caaaaccatt gttcacccac tgacgcggca acggcgcgag ctggttaaaa
    20341 tccagttcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc ccgttccagc
    20401 cagtaaccgc tcgcctgcgc ctgaatccgc gtttgtggtg acaacggaaa ataaggatgc
    20461 cagccggtac caaatggcag cgtctctgcc ccttgattgg tgacagagag cgtcaccgtc
    20521 agcgtatccg ccgttaaatg aaacgcctga cttacccgat agtgatagac accgctgcga
    20581 tgttcataca ccagacacaa actatcatcg ctgtgcgaga cacattgcca ctcgcccagc
    20641 cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag ttgatactca
    20701 cgcccctgcc agacaaaacg gttgccgctc acccggtttg caaatggcac cagcggaaaa
    20761 catgaggcat cggttgccac accgcttttt ttaccaggac gtaagagcgg cgtcgtatcg
    20821 cgccagaagc cttcgattac gccgccctgg tcagaaacgt ccagctttag cgacccgtgg
    20881 gataaggtat agatggtcat ccgctaactc cttaatccgg gaagttaatc acaactttcc
    20941 cgcatttgcc gctcgccatc agcacatagg catcacctgc ctgttctagc gagaagcgat
    21001 gggtaatggc gttacgcggc cacagcttcc agtcagtcaa atcgtgggcg catttttcca
    21061 tatggaacag actggtcacc caggagccga taatccgccg ttgatggtgc atcagatcag
    21121 cgctgacctc gaattccact tttccggttt caccaatgta aaccacccgc ccccagtcag
    21181 cagtggattg cagtgccagc aagcgacctg cggcattacc ggaacaatcg agcgcaacat
    21241 ccgcgccacc gtgggtaagt tcagcaatta tctgcggcag accttcggtg gttgctaaat
    21301 agccgtgatc catcccccct aactgttttg ccatcgccag acgttccggc agcatatcaa
    21361 cgccgatgat ccgttttgca ccgcgacctt tcgccagcat catcgccatc atgccgaccg
    21421 gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc aaaatccctt
    21481 cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc agcgcatccg
    21541 gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg ccgtcacgct
    21601 gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg cggcggcagt
    21661 tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc tctttaaaat
    21721 ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg ttgataaatc
    21781 cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga tagatatagt
    21841 ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg ttaatccccg
    21901 gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctagtaaa taagcagcca
    21961 gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct gcagcacccg
    22021 cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca gcaagatatt
    22081 cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa aggagttaat
    22141 accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa gcaatgtacc
    22201 gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg cggcgatgac
    22261 gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg ccgccaacaa
    22321 aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc gcactcgacg
    22381 aacgttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa aaaccgagcg
    22441 cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca tcatgatcag
    22501 cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct cgttttcgtc
    22561 aatcggcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcagag cgctccacag
    22621 ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc cggcgagcgt
    22681 tcccatcagc gcgcctaaca gcaacaccag cagacaagca atcgccaacg gaacttcaaa
    22741 ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa cgctgacatc
    22801 aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa tggtggcggc
    22861 atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt tcagggagaa
    22921 gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat ggcgactgac
    22981 aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag catacatctt
    23041 caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca gctcatccac
    23101 attgaccggc gagtgaaact cctgcgagaa cgtgccgtgc tgtaataaca ggatgcggtc
    23161 acacaccagc ggcaattcct ccacttcact ggagataaac accacgcttt ttccttcggc
    23221 agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga cgccgcgcgt
    23281 tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga tcaccacttt
    23341 ttgctgattg ccaccagaaa gcgtgccgat gggtgtttcg ctactggcag ccttgaccgt
    23401 catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca gcacaccgtt
    23461 ggtgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccta gccagggaat
    23521 gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca gcatgtcgcc
    23581 gtaatcgggg cgcatgattt tctcgccgtt gataacaatt tcgccctgtt catacgtctc
    23641 cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc cagcaatgcc
    23701 aagcacttcg ccacgacgta gcgtaaagct gatatcctcc agcttgggct tatggcgtaa
    23761 cgcacggact tccagtacgg cctgatccac aatttcctga ggggcaaccg gcgcaatatc
    23821 aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt tttcgagcat
    23881 cacatcgccc gccacctgac catcgcgcat aacggtggca taggaggcaa tgcggcggat
    23941 ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca tctttttcac
    24001 cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt catcaagaat
    24061 gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct tttgcgccgg
    24121 gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct gtaagcaacg
    24181 ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag gccattgacc
    24241 aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct gataaaccgc
    24301 acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt ccagtcgcgt
    24361 ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagca ttcgaatgag
    24421 agtcgatttg cccgcgccgt ttttacctaa cagcgcacga acttcgcctt tattgagcgt
    24481 gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg ccaccacttt
    24541 tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc agcgattagg
    24601 gcagagcaga ccatcaacgt gcgttgccag ccactgttta ccatcttccg ttttggtata
    24661 gagatcgatt ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa
    24721 tgtttgcgcg aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag
    24781 cacctgattg ttttccagct cctgagcaat ttcggttgtc atatccgaac cgaaaacagc
    24841 aatttttccg gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc
    24901 cgactccccc ataatggcgt tgagatccgg cgtggagata atcagttttt caccaacgga
    24961 aatcgcttta tctaaaacag tcccttcctg gttagcgaca atttgcgcgc cgggaacgcg
    25021 ggattttaat acttcttcaa atcctttacg tcgctgcaca caaacttcaa aggcttcgca
    25081 attgatgacg gcaattttcg gctggtcaat tttattggca ataaaataat cggcggcagc
    25141 gttacccagt ttttaccaaa ttccagtgga tcgccgacca gatccgccga gacatattta
    25201 tcgacaccct tttgattaat acaggtgttg tagcaaatca ccggaatacc cgcttcactg
    25261 gcgcgacgaa cggtacggct actgccattt tcagacactg ccgataaaat aatggcatcg
    25321 acattacgcg ccacgagggt atcaacaaag gtactttctt tcgaaatatc cccctgggcg
    25381 ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg aacgccctga
    25441 cgcactccag cgtaatatcc ctgggtatca aggtatattg caccaatggt catttcttta
    25501 tccgcagccc tggcaaatag tgcgctacct aatagcgtag ccattaataa tagattacgg
    25561 gttgttctca tttttttagg catagtgcct cctgtagggt ttttattaac aacggcttat
    25621 tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa atattacagt taatagggtt
    25681 tagtgaacgt agttaaaaat aaaaggcatc acagtggtga ataatgccgc tttcggccag
    25741 gccggattaa tcgccgtcaa ttcagcgcga gcagactcat attgcgcaac ctccccgagt
    25801 cccgcggacg ccagcatcgc caccatcagg catttttctt tatgctgctg ttgtaaatcg
    25861 ccatacagcg ataacaggtc aggttgtgaa acagcaaaga aatccgcctc aatactggtt
    25921 ttcgccatct cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc ggtttgctgt
    25981 tcacccagca gtcgcagcgc cattccttgc cagaagagat aatcaaccgg ctgatcgttg
    26041 taataactgt ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcatc
    26101 gcttcagttt catcgccctg cgcgttggcg catatcgcct gccagaacca gatgtcgtta
    26161 tcagtttgcc ccggtaaacg gccttcgctt aaattctccg gataatacag cgcggcatga
    26221 agcagttcgc tggcctgctg cggctctctg gcatcaagat gctgccaggc gcgtaataac
    26281 tggttgagga taaactgact ggtgaccttc ccttccccgc cttcccacgg gtggaattta
    26341 cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg tgagatgcca caaattgagc
    26401 agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcca gattattttc cagacgcgcc
    26461 agtcgtttct ccggtgtggc tccacttaac ttatccagca aatcccgttc gaaaagcaga
    26521 cgtgcatcct gcggcgcaag ctgataagca ttatcaagat aacgcgcggc cagctcataa
    26581 tcgtgtttct tattccacgc atggatcgct aacccgcgcc agccgtcggc aaactccggc
    26641 gacatctcta cgcaacgttg ccataaggca atggctttgt tgtagctacg tttgttgtag
    26701 tagaagcagg ccagtaaatg gcgagcaaac caacactctt caatactctc cagcgccgcc
    26761 acttcttcca gcgtattcgg gaaacggaca aactgcggga agacatcaat ggcttttgcg
    26821 accagttcgc cacgttcggc tttcggcagc aagctggctt gcaggtaaag cggcagcgtg
    26881 cgctggcagt ccagtgcgtt cagcatctct gccgccaggg tgggcattcc ccagttaatc
    26941 agttgcccgg cggtcatcag agcgttaacg tcgcgtccct gacacagacc gcgccactgc
    27001 gcgagggctg attcgctacg accatcgaac cagttcagcc accacagagt ggcgttcagc
    27061 ggataatcac gcagcagttt ctcgcgctgc aaacgcgcgt tgtcctgacg accacttaac
    27121 accagcagca gattatgcaa gcaaagcact tcctgattgg ttgggcaggc acgaagactt
    27181 tgttggcaaa aatccagacc cgcgtcgaag ttatcattac gcgccgccag tcgtgccaga
    27241 ccataatagc cgcctgcttt actattgccg ctccagaccg cacgccagaa atcctcttcg
    27301 gcttgttgat attgtccctg acgttcgtaa gcactggcgc gaatcaaact tgcttgtcca
    27361 cactgcggtt ttttgttcag cgcatgtgca cgtttcagcg cctgactggc atacgccacc
    27421 gcttgcggga aatctgcgcg gttatattcc agcatcgcca gcgccaagtt acagcgataa
    27481 tccagcgggt ccagcgccac gccgcgcagg tagtaatcga acggtgaacg gctggcgtga
    27541 tgatattgct ccagatgctg accgataaac caggcttcat ctgtactggt aatgtcttgt
    27601 gctgccagtg gcgctttggc gacgtccggc aacggcaact cctgcggctg atgttcctga
    27661 taactcagta caatattgcc gtcggcatcg gagagttcaa tggtcagtct gtcaggattg
    27721 ataccgtgca acacgccctg gatggcggtc gctggtgtca gggccacggc atcatcgagc
    27781 aacgcgttgc atttgccgat ttcgcggatc gccagacggt atccgttcaa cggagagatg
    27841 gcatacagcc cccactcaat cccccgctca ctacgctgga gttttatcac cgcatcgcgg
    27901 gaggcatttt gcaccatgcc cagagaatga taaggcagga aatactgctc gaaacgcttc
    27961 tcttcgtaag catcaagcca ggtaaaatca ggctggttat cggcaaaaat accggtcatc
    28021 agttcgatat acgggccgtt attgtcggtc agactcttat cccacgcctg gccaaattca
    28081 ctgtgtcccc aactccactg ttttttacct ggcgcaatat ggtggttggc aacgtgcagc
    28141 aaaccgccat cttcatcgtg acaccacgcg ccgacaaaat cgtactgcga tttttcggcc
    28201 atatatgagg ttggaacagg cacattttta tagcgagaaa tgtccactcc ggcggagtag
    28261 tccactttgt agtaagtgcc ggtggcgatg gggaaagcgg agacggcccg tttgccgtga
    28321 tcaaacaccg cagtcacatc cggtgggaaa acgctctgat gcccttcacc ccctttcact
    28381 gccgggttga cccaccacaa gaaatgacgc ggcgtggcgt tgccgttata gacgcggctg
    28441 gcgatttcca gtgccgcccg gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca
    28501 tgcatcggct ccgtttcgcc gacccacacc gtctgtgcac cgtcttcatg ggcttcgagg
    28561 gtgaaatcaa cgggcataaa ggtggtcggg cgatggtgtt gcggccagtt aaactcaatc
    28621 ccgccggaga tccacggtcc cagcatcccc accagcgcag gtttaatgac ttcattgtga
    28681 tagacaaaat cgcgctgttt cactttatcc catgcgcgat gcacccgacc gcccagttcc
    28741 ggcaggatca tcactttgat gtagtcgttt tccagccaca ccgcctgcta ggatttcagg
    28801 gttttctgct cgctcagcgt atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc
    28861 tgataaacgc gattttccag gaacatggga tgtatatcct gcggcccggt ttcataggtc
    28921 gggatctcaa cgcgctcttg ccacactttt actggagtca tggtgccctc aatattaaca
    28981 agacatactg aattaaaaga tttgtggcag tgtattgaac aatctggcaa tgttttcgcg
    29041 gaataatcac gcaattaact aaacaaggtt tagtgaagat gagagcctgc attaacaatc
    29101 aacagattcg ccaccataac aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg
    29161 ccaataaatc aacgctggcc cggctggcgc aaatttcgat tccggcagtc agtaatattt
    29221 tgcaggaact ggaaagcgaa aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg
    29281 ggcatagtag cggtacatgg ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg
    29341 tgacgcccac cagtattgag tgtcaggttg ctaatgcttg tttaagtccg aaaggcgaat
    29401 ttgagtattt acagattgat gtaccgacgc cgcaggcgct gctgtccgaa atcgaaaaat
    29461 gctggcatcg ccaccgtaaa ttgtggccgg accgcaccat caatctggcg ctggcaatcc
    29521 acgggcaggt tgatccggtg accggcgtat cgcaaaccat gccgcaagcg ccgtggacaa
    29581 cgccagttga ggtgaagtat ctgctggaag agaagctggg cattcgggtg ctggtcgata
    29641 atgactgcgt gatgctggcg ctggcggaga aatggcaaaa taattcgcag gaacgggatt
    29701 tctgcgtgat caacgttgat tacggcattg gctcgtcgtt cgtgattaac gagcaaattt
    29761 atcgcggcag tttgtatggt agcggacaga ttggtcacac catcgttaat ccggatggag
    29821 tcgtctgcga ctgtggacgt tacggcagcc tggaaaccgt cgcctcgtta agtgcattaa
    29881 aaaaacaggc gcgggtatgg ctaaaatcac aaccggttaa tactcagctt gagcctgaaa
    29941 aactgacaac cgcgcagtta atcgctgcct ggcagagtgg agaaccgtgg atcaccagct
    30001 gggttgatcg ctctgccaat gccattggtt tgagtctgta taacttcctc aacatcctca
    30061 atattaatca gatttggttg tacggtcgca gttgtgcctt tggtgagaac tggcttaata
    30121 ctattattcg ccagacagga tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag
    30181 cgacgcaaat tggcttcggg caattaagcc gcgcacaaca ggtgctggga attggctatt
    30241 tgtatgttga ggcgcagtta cgacagattt gatggcgcga taacgtagaa aggcttcccg
    30301 aaggaagcct tgatgatcat aaacgaaaaa ttgcctgatg cgctacgctt atcaggccta
    30361 cacggagatt gcaatatatt gaatttgcaa gattttgtag gcctgagaag gtgttcatgc
    30421 cgcatccaac atgaacaacg agcacattgt cagcaaatca ccgtttcgct tatgcgtaaa
    30481 ccgggtaacg tgcgcagatg tcgagaactt tacctttgat gcgctcgata acggcttcat
    30541 cattgatgct gtccagcacg tcacacatcc agccagccag ttctttcgct tcggcttctt
    30601 taaagccgcg acgggtaatc gccggagtac ctacacgaat accggaggtc acaaacgggc
    30661 tcttcggatc gttcggtacg ctgtttttgt tgacggtgat gttagcacgg cccagagcgg
    30721 cgtctgcttc tttaccggtc aggtttttat caaccagatc aaccaggaac aggtggttat
    30781 cagtgccgcc ggaaaccact ttgtagccgc gctcgaggaa cacttctacc atcgctttag
    30841 cgtttttcgc gacctgctgc tggtaagttt tgaactcagg ctccatcgct tctttcagag
    30901 caaccgcttt accggcgatt acgtgcatca acggaccgcc ctgaccacca gggaaaacgg
    30961 cagagttcag ttttttgtac agctcttcgc taccaccttt cgccaagatc aggccgccgc
    31021 gcggacccgc cagggttttg tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt
    31081 tcgggtagac gccagcagca accaggcccg caacgtgcgc catatcaacg aacagataag
    31141 caccgatgct gtcagcgatt tcacgcattt tcgcccagtc aaccacgccg gaatatgcag
    31201 agaaaccacc gataatcatt ttcggcttgt gttctttggc ttgtttttcc agatcggcgt
    31261 aatcgatatg accggtagca tcgataccgt aaggaacgat gttgtacagt ttaccggaga
    31321 agttaaccgg agaaccgtga gtcaggtgac cgccatgcgc caggttcata cccagaacgg
    31381 tatcacctgg ttccagcagc gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg
    31441 gctggacgtt agcgtagtca gcgccgaaca gttctttcgc acgatcgatc gccagttgtt
    31501 caacgatatc aacatactcg caaccgccgt agtagcgttt gcccggataa ccttcagcat
    31561 atttgttggt cagctgagaa ccctgcgcct gcattacgcg cgggctggtg tagttttcgg
    31621 aggcgatcag ttcgatgtgc tcttcctgac gtactttttc ctgctccata gcctgccaca
    31681 gttcggcatc ataatcggca atgttcattt cacgctttaa catccgcatc tcctgactca
    31741 gctaacaata aaatttttgg cctttatagg cggtcctgtt ggacaacggc gaacagtata
    31801 accgaatcat tgtgcgataa caggtcttga caaaggaatt tacgcaaacg attaccttca
    31861 ggctacgcaa ggctttggag aataaagagc ttgcaaccgg aaacggattt cttttcaggt
    31921 ttgtgatgca aatttttcac ttcatcacat tctttctgaa aaacaccaaa gaaccattta
    31981 cattgcaggg ctatttttta taagatgcat ttgagataca tcaattaaga tgcaaaaaag
    32041 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
    32101 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
    32161 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
    32221 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
    32281 gtagaaaaaa tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc
    32341 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
    32401 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
    32461 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
    32521 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
    32581 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
    32641 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
    32701 cgtatcgcgg tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc
    32761 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
    32821 gatgacacac cagtgacgtt aatctctacc ggtgttggtc aaacgccaat gctggcaatg
    32881 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
    32941 aatggcgaag ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc
    33001 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
    33061 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
    33121 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
    33181 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa
    33241 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
    33301 tcctcttcac cggtacggat gcgaatgacc cgagccacgt caaagacgaa gattttaccg
    33361 tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg
    33421 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
    33481 ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc
    33541 atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt gaagggtttt
    33601 ataatcgcat caatcttttt catgctattc cttgaaaagg ttgcctgtct tttgatctgc
    33661 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
    33721 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg
    33781 ttgcctttgg tgatttgcag cagcttacgc aagtagttga gttcaaaatg attacgtgcc
    33841 tcaacaaagg ttggcagcgc cgtattttca ccctccagcg cctgctccac cagcgcatca
    33901 ctaatcaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
    33961 acattacccg gccagctcgc ggtcatcagg cgtttcatcg catcggtaga gaacgcgcgg
    34021 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg
    34081 tcttctgtgc gctccgccag tgccggaatt ttcaggctga caacgttgag gcggtaatag
    34141 agatcttcgc ggaattcccc gcgcgccatc gcttttgaca gatcacggtg agtggcagaa
    34201 ataatccgca cattgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
    34261 tgcaacacgc gcagcagttt gacctgtaac ggcgcaggca tatcgccaat ttcatcgaga
    34321 aatagcgttc cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
    34381 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca
    34441 cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat agcctgggcg
    34501 aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc tgattgcgcc
    34561 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aattgcctcg
    34621 cgccagcgtt catcggtggc tggcgcggat tgctccagcg catcgtcaat tgcctgatat
    34681 agcgcatctt tatcgacagg cttggtgagg aaactaaaaa cgccctgctg tgttgcagca
    34741 acggcatcgg ggatagaacc atgcgcagta agaataatta ctggcattcc cggctgcact
    34801 ttctggattt cagcaaatag ctgcataccg tccatttcat ccatccgcag gtcgctgata
    34861 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
    34921 accacactgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
    34981 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
    35041 tggcgcgggc ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
    35101 ggtcgagagc tggcgttcaa tatcggtcag gttttccagc ttgcgggtgg tgagttccag
    35161 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggactg
    35221 ctggagttta ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
    35281 gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta acgcttcaat
    35341 acgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
    35401 ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgcgactg
    35461 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    35521 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    35581 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgattctg
    35641 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
    35701 tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg
    35761 ttgcagacag tctggacatg gacagcgcgg agaaaccggg gcgtacatat cgtacgtgaa
    35821 gatttcgagc actacccggg gccgaactga caaataaaat agcctgatag gataggctgt
    35881 tagcattatt tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg
    35941 ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt
    36001 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt
    36061 tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata
    36121 taaacccgcg caccatgtaa actgctgcga aggcaaatgt taccggattc agccccgtag
    36181 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
    36241 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
    36301 tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag ttccaccgca
    36361 ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcag
    36421 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc
    36481 tgatcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga
    36541 gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac
    36601 caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cacagaacga aacgctattg
    36661 cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg
    36721 ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat
    36781 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
    36841 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga
    36901 cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc
    36961 aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
    37021 agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
    37081 agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca
    37141 ctacgccggg catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    37201 ttcaggcttt gccaggcttg ccatgccagc accaacaggg gtagtagaat tagcaaaaat
    37261 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    37321 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    37381 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    37441 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    37501 gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta
    37561 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt
    37621 gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    37681 aaagagacaa cttaagataa acttattaga ttacaataaa atcaatcatt taagtgtcgc
    37741 cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg
    37801 agttgggagc ttatgatagt ggttggtgct taatgccgca tccgggctgc aaaaccaatg
    37861 aactgatgac ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat
    37921 cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt
    37981 gcggcatcat aatggtgact cgaccacttt cagtcgtaac cgccgtaata ccgttcggtg
    38041 aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg acatagcgca
    38101 gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc acttccacgc
    38161 gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacagca
    38221 gagacgggct ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtgt
    38281 tacgcacaaa acgtggccac aactcactcc ccgggatcag ttcacgcagg ttagacatca
    38341 tctggcaacc gttacatacc cccagggcca gcgtttgcgg acgatggaag aaggttgcaa
    38401 actcatcgcg tacacggtca ttgaacagga tagacttcgc ccaaccttca ccggcaccca
    38461 gcacatcacc gtaggagaaa cctccgcacg cgaccagggc gtgaaaatcc tccagtcccg
    38521 tacgtccagt cagcaggtcg ctcatatgca cgtcgatagc atcaaagccc ccacgttgga
    38581 aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagtacg gcaactttcg
    38641 gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg aacgacagtt
    38701 ttacattcag gcccggatcg gcgtcgttag atttcgcctg atgctcctga tcggcacact
    38761 ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg
    38821 tagtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg
    38881 aaaccgcctg cccaacataa tggacgcaat ccgcaagccc atgctgtgcc agtacggact
    38941 cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc acccagttct tcgttaaaca
    39001 acgccgccag gcgatcgtca cccagagagg cgatatccgc gtcaatgcca caatgacctg
    39061 caaaggccat ttccgccagc gttaccagca ggcctccatc agagcggtcg tgatacgcca
    39121 gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttggacaa
    39181 catcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg
    39241 cgcccagcgc gttattgcct ttgcccaaat caatcagcag cagtgcgtta tcttcggtac
    39301 aaagctgcgg cgtgatggtg tgacgtacat cttccacgcg ggcaaatgca gaaatcacca
    39361 gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg
    39421 acatagagtc tttacccacc gggatcgtca ggcccagcgc cggacaaagt tcttcaccca
    39481 cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc
    39541 agttggcgga aagtttaatg cgtttgatat cgccaatctg tgttgcggca atattggtta
    39601 acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac
    39661 gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc
    39721 agttagcgac cggcacctgc cacggcccca ccatctgatc gcgcgccacc atgccggtta
    39781 cgctgcggtc accgatggtc accaggaaag ttttctccgc cacggtcggc agatgcagta
    39841 cgcgtttcac cgcgtctgca atggtgatcc cttcacggac cagcgcatcg cccttcgctt
    39901 tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg tccagcggca
    39961 gatcgatcgg ctgattatca aaatgacgat cgtgcagaga aagatgcagt tcttcggtcg
    40021 cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca cagttcgtca aacagcggta
    40081 attgatcggc agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca
    40141 gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag ttcaaattta ccgccgcgtc
    40201 cgccgtcgct caccagctcc ggcatcgcgt tagaaagacc gcccgcaccg acgtcgtgga
    40261 taaacagaat tgggttagca tcaccaagct gccagcaacg gtcgatcact tcctggcaac
    40321 gacgctccat ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact
    40381 gaccagacgc catggaagac gccgcgccgc cgccaagacc gatgttcatt gccgggccgc
    40441 cgagaacgac cagcttcgca ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa
    40501 tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccat
    40561 tgtggctgtt cactttttct tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa
    40621 attcgttgtt aaacgccgca ccgcccagcg ggccttcggt catgatgtcc agcgcggtaa
    40681 caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagcca ggaattcgca
    40741 ggttggatac ggagaaacca accaaaccag ctttcggctt cgcgccgcgc ccggtggcac
    40801 cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg
    40861 tcgggtggtt gtgagtttcg actttcatca ggatgtgcgc cggttcctga tggaaattgt
    40921 agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc attacggcgg
    40981 cgttatcttt ataagcagag agaacgtgat ctggcgtggt ttcgaaagta tttttgatca
    41041 tcctgaacag cgatttcggc tgttgttcac cgtcgataat ccagtcggcg ttaaaaattt
    41101 tgtggcggca gtgctcagag ttcgcctgag cgaacatata cagctcgatg tcgttcgggt
    41161 tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc gccagagcca
    41221 agccaagacg caggttagcg tcgatcagcg cctgacggcc ctgccccagc aaatcaacgc
    41281 ttgtgaccgg agtcggttga tggtgggcaa acaactgctc tgcatcatct aaatcaaaaa
    41341 agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg
    41401 tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta
    41461 gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac
    41521 gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc ggaccatatt
    41581 tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa
    41641 aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga aaacgtgcca
    41701 gcagtttgtt gattcggaat gccgacagtg caggcgaacc acgcagaatt tccatcataa
    41761 gtctctcgtc ttctaagctt tcggtgtacc caaggggggg gaaacgggcg tcattataaa
    41821 gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca
    41881 acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg
    41941 ctcatttatg agtaaacctt tcactattat tacgtttttt caagctggga cgcgcacgac
    42001 acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg gcattctggc
    42061 actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag ccgacaaccg
    42121 tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata ctcccctgac
    42181 ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga aacagtttgc
    42241 cgattacctc ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc agctgtttga
    42301 cgaccttgat aatggtaacg ccgacctgct ggcggcagga ctggtctata acagtgagcg
    42361 ggtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac tggtttataa
    42421 agtgggtcag tatcgcccac gtacgctggg caacctgacg gcggagcaac tcaccgttgc
    42481 accgggtcat gtggtagtta acgatctcca gaccctgaaa gaaacaaaat tcccggaatt
    42541 aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg tcatcgaagg
    42601 aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc
    42661 ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacttggt ttagcccgtt
    42721 agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga
    42781 cggtacgctg gcacgcattg aagagaaata cctggggcat ggcgatgatt ttgattacgt
    42841 cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt
    42901 tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt atcaggaatc
    42961 gcactgggat gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa
    43021 aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga gcatcagcgg
    43081 tggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc cggagaacga
    43141 acggatctgg tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg
    43201 cgccctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa aacagcgtct
    43261 gcctttactt agccagaaac cctattacag caagctgact tacggctacg ctcgtgggca
    43321 tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg tgggttatct
    43381 gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg attatccggc
    43441 ggtatcgcct acggagttgg gcaaagagaa atttcctttt ctctcgtttc tttcccagtc
    43501 gtcatcaaac tatttgaccc attctccctc tctgctgttt tccaggaaag ggagtgaaga
    43561 gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt cctggcggcg
    43621 catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc cttccgtaat
    43681 ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc ccgcagcgcc
    43741 agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga tcatcgctcc
    43801 ggcacacatt acacatggtt caagcgtgac atacaacgtg gcgtcgatca gacgataatt
    43861 ttgcatcacc agaccaccct gccgcagggc catgatttct gcatgtgcgg tgggatcatg
    43921 gcgaccaatc gggcggttcc agccttcgcc gattacccga ttgttatgca ctaataccgc
    43981 gccgaccggc acttcccgct catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat
    44041 ccaatattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta taaaagcgcg
    44101 gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt caccgcgcgg
    44161 ggttactcgc caacgatgct gacagaaata aagcagcgga ttgtcctgat tactgtcgct
    44221 atagccactg tacagccgca gcggcgtgcc gattttgcgc tccagttgtg cgaccttttc
    44281 atgtcccaga caacgcatcg tcaataccca accaccatag ccacgctgaa tttggctggc
    44341 gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa ccagcggctg
    44401 cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg tggttaatcg
    44461 ctcctgaacc agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg
    44521 caacgtctgt aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa gcagactcat
    44581 cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctataatcg gtaacaacgg
    44641 caggacaagt aacgcattca gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa
    44701 catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac gctcgtgagt
    44761 tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac tacatagcct
    44821 aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa ctggaatttt
    44881 taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtgt ctcatattgc
    44941 ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc gtatccgtca
    45001 gcgttaccaa gggcttgccc agagtgataa aaaactggcg gattatctgc tgctacaacc
    45061 tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag tcagtcagtc
    45121 cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc ttaagttggc
    45181 gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc ataaccaaat
    45241 ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa ataccgccgc
    45301 tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg taacaatgtt
    45361 gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa
    45421 ctttgcctgg aagctgatga agattggctt caatgctgcc gcactgcgcg atatgcatgc
    45481 gctactcgca acagtacagg cgtcgtcccc tgacgatctg ttattagcca tttcctacac
    45541 cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg gcggaaaagt
    45601 gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc attgcctgta
    45661 taccattgcc gaagaacaag cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg
    45721 aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac tggcaccaga
    45781 acgtattcgt catagtgaag cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat
    45841 aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgctgtta atcactaaaa
    45901 aatgcatcaa ttgtgatatg tgtgaaatca aataaaaaca catttatttt catagtgata
    45961 cagcaatgtg ttattgaatg tgttgttgga tttatggtgc aaaaaaattt tgcataagtt
    46021 cagcaacaca aagaacacca ctttctttaa cgtcctccgc gccctaaagg acggggcttt
    46081 acggcgcaaa aggcctgtga catacaacat taacttttca gtcaaccgta gcacgttctt
    46141 gtatacgccg tgtctgcggt tttatataac cctggctgga aacctcttat acaaagtcga
    46201 ttcgccaaca tcatagatga tcgccacctt ctggcgagaa actcctgatg caattagtcg
    46261 cccggcctgc gcccattatt ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg
    46321 tgcgcgagca gcttccagtc ctgcttttgt tcgttcaaca atcagttcac gttccatttc
    46381 agccagggca cccatcacat ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga
    46441 atcagtcaga ctacgaaagt tgatgcctcg ttcgcgcaac gcctccacca gcacgacaag
    46501 atgccgcata ctgcgcccca gccgatccag cttccagaca accagtgtgt cgcctgccga
    46561 taatgtcttg agcagttttt tcagccccgg tctttcggac tttgtaccac ttatcttgtc
    46621 ttcaaaaatc agctcgcatc ctgcacagtt caacgcatta cgttgtagat cggtattctg
    46681 gtcatttgtt gatacgcgta catagccaat aagcatggt
//
LOCUS       DAJVPT010000018        46471 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVPT010000018 DAJVPT010000000
VERSION     DAJVPT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(197..272)
                     /locus_tag="OQM47_001003"
     tRNA            complement(197..272)
                     /locus_tag="OQM47_001003"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Lys,seq:ttt)
     gene            complement(437..1228)
                     /gene="cpoB"
                     /locus_tag="OQM47_001004"
     CDS             complement(437..1228)
                     /gene="cpoB"
                     /locus_tag="OQM47_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1479251.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1238..1759)
                     /gene="pal"
                     /locus_tag="OQM47_001005"
     CDS             complement(1238..1759)
                     /gene="pal"
                     /locus_tag="OQM47_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1479252.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1794..3086)
                     /gene="tolB"
                     /locus_tag="OQM47_001006"
     CDS             complement(1794..3086)
                     /gene="tolB"
                     /locus_tag="OQM47_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1479253.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3219..4460)
                     /gene="tolA"
                     /locus_tag="OQM47_001007"
     CDS             complement(3219..4460)
                     /gene="tolA"
                     /locus_tag="OQM47_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1479254.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4525..4953)
                     /gene="tolR"
                     /locus_tag="OQM47_001008"
     CDS             complement(4525..4953)
                     /gene="tolR"
                     /locus_tag="OQM47_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1479255.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4957..5649)
                     /gene="tolQ"
                     /locus_tag="OQM47_001009"
     CDS             complement(4957..5649)
                     /gene="tolQ"
                     /locus_tag="OQM47_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1479256.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5646..6050)
                     /gene="ybgC"
                     /locus_tag="OQM47_001010"
     CDS             complement(5646..6050)
                     /gene="ybgC"
                     /locus_tag="OQM47_001010"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1479257.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6200..6493)
                     /gene="ybgE"
                     /locus_tag="OQM47_001011"
     CDS             complement(6200..6493)
                     /gene="ybgE"
                     /locus_tag="OQM47_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1479258.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6493..6606)
                     /gene="cydX"
                     /locus_tag="OQM47_001012"
     CDS             complement(6493..6606)
                     /gene="cydX"
                     /locus_tag="OQM47_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1479259.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6621..7760)
                     /gene="cydB"
                     /locus_tag="OQM47_001013"
     CDS             complement(6621..7760)
                     /gene="cydB"
                     /locus_tag="OQM47_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1479260.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7776..9344)
                     /gene="cydA"
                     /locus_tag="OQM47_001014"
     CDS             complement(7776..9344)
                     /gene="cydA"
                     /locus_tag="OQM47_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1479261.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10193..12826)
                     /gene="mngB"
                     /locus_tag="OQM47_001015"
     CDS             complement(10193..12826)
                     /gene="mngB"
                     /locus_tag="OQM47_001015"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1479262.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12844..14760)
                     /gene="mngA"
                     /locus_tag="OQM47_001016"
     CDS             complement(12844..14760)
                     /gene="mngA"
                     /locus_tag="OQM47_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1479263.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14930..15652
                     /gene="mngR"
                     /locus_tag="OQM47_001017"
     CDS             14930..15652
                     /gene="mngR"
                     /locus_tag="OQM47_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1479264.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15755..16624)
                     /gene="sucD"
                     /locus_tag="OQM47_001018"
     CDS             complement(15755..16624)
                     /gene="sucD"
                     /locus_tag="OQM47_001018"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1479265.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16624..17790)
                     /gene="sucC"
                     /locus_tag="OQM47_001019"
     CDS             complement(16624..17790)
                     /gene="sucC"
                     /locus_tag="OQM47_001019"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1479266.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17884..19101)
                     /gene="odhB"
                     /locus_tag="OQM47_001020"
     CDS             complement(17884..19101)
                     /gene="odhB"
                     /locus_tag="OQM47_001020"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1479267.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19116..21917)
                     /gene="sucA"
                     /locus_tag="OQM47_001021"
     CDS             complement(19116..21917)
                     /gene="sucA"
                     /locus_tag="OQM47_001021"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1479268.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22218..22934)
                     /gene="sdhB"
                     /locus_tag="OQM47_001022"
     CDS             complement(22218..22934)
                     /gene="sdhB"
                     /locus_tag="OQM47_001022"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1479269.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22950..24716)
                     /gene="sdhA"
                     /locus_tag="OQM47_001023"
     CDS             complement(22950..24716)
                     /gene="sdhA"
                     /locus_tag="OQM47_001023"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1479270.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24716..25063)
                     /gene="sdhD"
                     /locus_tag="OQM47_001024"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24716..25063)
                     /gene="sdhD"
                     /locus_tag="OQM47_001024"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1479271.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25057..25461)
                     /gene="sdhC"
                     /locus_tag="OQM47_001025"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25057..25461)
                     /gene="sdhC"
                     /locus_tag="OQM47_001025"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1479272.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25842..25949
                     /locus_tag="OQM47_001026"
     CDS             25842..25949
                     /locus_tag="OQM47_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479273.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26155..27438
                     /gene="gltA"
                     /locus_tag="OQM47_001027"
     CDS             26155..27438
                     /gene="gltA"
                     /locus_tag="OQM47_001027"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1479274.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27829..28395
                     /locus_tag="OQM47_001028"
     CDS             27829..28395
                     /locus_tag="OQM47_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1479275.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28446..30905
                     /locus_tag="OQM47_001029"
     CDS             28446..30905
                     /locus_tag="OQM47_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1479276.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30920..31651
                     /locus_tag="OQM47_001030"
     CDS             30920..31651
                     /locus_tag="OQM47_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1479277.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31648..32724
                     /locus_tag="OQM47_001031"
                     /pseudo
     CDS             31648..32724
                     /locus_tag="OQM47_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32876..33922
                     /locus_tag="OQM47_001032"
     CDS             32876..33922
                     /locus_tag="OQM47_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1479278.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33919..34710)
                     /gene="nei"
                     /locus_tag="OQM47_001033"
     CDS             complement(33919..34710)
                     /gene="nei"
                     /locus_tag="OQM47_001033"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1479279.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34746..35480)
                     /gene="pxpA"
                     /locus_tag="OQM47_001034"
     CDS             complement(34746..35480)
                     /gene="pxpA"
                     /locus_tag="OQM47_001034"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1479280.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35470..36402)
                     /gene="pxpC"
                     /locus_tag="OQM47_001035"
                     /pseudo
     CDS             complement(35470..36402)
                     /gene="pxpC"
                     /locus_tag="OQM47_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36396..37052)
                     /gene="pxpB"
                     /locus_tag="OQM47_001036"
     CDS             complement(36396..37052)
                     /gene="pxpB"
                     /locus_tag="OQM47_001036"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1479281.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37075..37818)
                     /gene="ybgI"
                     /locus_tag="OQM47_001037"
     CDS             complement(37075..37818)
                     /gene="ybgI"
                     /locus_tag="OQM47_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1479282.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38089..39570
                     /gene="dtpD"
                     /locus_tag="OQM47_001038"
     CDS             38089..39570
                     /gene="dtpD"
                     /locus_tag="OQM47_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1479283.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39720..41138)
                     /gene="phrB"
                     /locus_tag="OQM47_001039"
     CDS             complement(39720..41138)
                     /gene="phrB"
                     /locus_tag="OQM47_001039"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1479284.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41135..41644)
                     /locus_tag="OQM47_001040"
     CDS             complement(41135..41644)
                     /locus_tag="OQM47_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1479285.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41773..42060
                     /locus_tag="OQM47_001041"
     CDS             41773..42060
                     /locus_tag="OQM47_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1479286.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42115..42495)
                     /locus_tag="OQM47_001042"
     CDS             complement(42115..42495)
                     /locus_tag="OQM47_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479287.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42498..45884)
                     /locus_tag="OQM47_001043"
     CDS             complement(42498..45884)
                     /locus_tag="OQM47_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1479288.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDRHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45903..46106)
                     /locus_tag="OQM47_001044"
                     /pseudo
     CDS             complement(45903..46106)
                     /locus_tag="OQM47_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11172 a        12650 c        11216 g        11433 t
ORIGIN      
        1 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
       61 cgacactacg ctggatacta caactaacta tgttagtggg ggtcgtgcag gatgactcga
      121 cttcgtctcg cccttcggcg ccgttgctga tgcaacgtta tccttcacgt tttactaccg
      181 cttccacctt agcgattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
      241 gtcaactgct ctaccaactg agctaacgac ccactttttc gttgctttcg gtttgtttga
      301 tatcccgtgg caacggcggc atatattact gatttcagac ttgagcgcaa caaaaatttc
      361 gatgcagatc actcaactgc ttatgattcg cacgacacga ccagaaataa tgcgacttct
      421 ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg tgcctgttta gcgccatcag
      481 taccagggta tttactgata acctgctggt atacggcttt cgcttttgcg gtgtcacctt
      541 tgtcctgcat gatgacgccg actttaaaca tcgcatcagc agcctttggt gacttcggat
      601 agtttttcac taccgaagca aaatagtacg ccgcatcatc ttttttaccc ttgttgtagt
      661 ttaactgacc cagccaataa ttggcgtttg gcaggtaagt tgaatcaggg taatttttga
      721 tgaaattctg aaatgccacc attgcgtcat cctggcggga tttatcctgc accagcgcaa
      781 tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac cggcgcgcca gcattcgcag
      841 taccagcatc agctgtcggc gtcgttgatg ccgtcgcacc gctttgatcg ctgctggttg
      901 attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg caacaggatc tgcttttgcc
      961 gctccacgac ctgattcagt tgatactgat tttcctgaat ctgaccacgc agggaatcaa
     1021 tatcggattg attatcagaa agttgttgct ggagttgggt taaaagctgg ctgtgagcat
     1081 tagaaatacg ctcaagttga gtgacgcggt cttcgaccga gcctgagctg acactactga
     1141 ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc aaccagtaac gacagactca
     1201 atagttgatg tctgaagtta ctgctcatgc aattctctta gtaaaccagt accgcacgac
     1261 ggtttttgga gtatgccgct tcgtcatgac ccagtactgc aggtttttct ttaccgtaag
     1321 aaacgatgga gatctggtct gcagaaacgc ctttaccctg caggtacatc ttaacggcgt
     1381 tcgcacgacg ttcacccagg gagatgttgt attccggagt accacgttcg tccgcgtgac
     1441 cttctacggt gactttgtaa gacgggttgc tacgcaggaa gtttgcatgt gcatccagca
     1501 tttgagcgaa gtcagaacgg atatcgtact tgtccagatc gaagtaaacg atgttgttct
     1561 gctgcagctg ttgcatttgc agacgagcct gctcttcgga agacatgttg ccgttgccgc
     1621 cgttcgcatc cataccagtg ccggcaccca gcatgccttc gctgccgtca ttgctggcgt
     1681 tcttgttgga agaacatgcc gcaattgcca taacaggcag agcaatcatc agccctttca
     1741 gcactttgtt cagttgcatt tcaatgattc ctttactatt caattaatta ttatcacaga
     1801 tacggcgacc aggcagggaa tttgacctgt ccatcagttg ccggaagacg cgctttgaaa
     1861 cgcccatctg tagaaaccaa attcagcacg gatcccatcc cctgagaaga gctgtagatt
     1921 accatagtgc cgttaggtgc cagacttggc gtttcatcca ggaacgtgga cgacagaact
     1981 tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct gctgcccacc attggagctg
     2041 accattacca taaatttacc gtcgctgctg acatccgcat cctggttctg cgaaccttcc
     2101 caggtaatac gttgtggcgc accgccgttg atattcactt tataaacctg cggacgaccg
     2161 gcctggtcag aagtaaatgc caggttctgg ctatccggga accaggtcgg ttcggtattg
     2221 ttactgcgac catcagtcac ctggcggatc tgaccagaag ccaaatccat tacgtacagg
     2281 ttcagactac cggttttcga caaggcgaat gccagtttgc tgccgtctgg cgagaatgca
     2341 ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta cagcgccatt tgccagcgtc
     2401 tgaataacca gcgcggaacg accgctttcg aaggtcacat aagccagttt agaaccgtct
     2461 ggtgaccacg ccggagacat cagcggctgc ggtgaacggt gaacgacaaa ctggttgtaa
     2521 ccgtcatagt cagatacgcg cagttcatac gggaactgac cgccgttggt ctgaacaacg
     2581 taggcaatac gggtacggaa cgcaccttta atgccggtca gtttttcaaa cacttcatca
     2641 ctggcggtat gaccagcata acgcagccac tgcttgttca ctttgtacga gttctgagca
     2701 agtacagtac ccggtgcgcc gccagtgtca acaagttgat aagcaacatt gtaagaacca
     2761 tccggattcg gagtgacttg accgacaact acagcgtcaa tgcccagtgc ggaccatgca
     2821 gctggttgta cttcctgcgc actacccggc tgctgtggca gacgagcgcg atctaacgga
     2881 ttaaatttac cgctgttacg caagtcagca gcaacgatgc cgccaatatc ttcaggtgcc
     2941 gcaccaggcc ccgcccactg gaaaggaaca acaccaatag gacgaccgga atctacaccg
     3001 ctgtcgatca caatgcggac ttcagcatgc agaactgatg cccacagtat gagaaaacca
     3061 aatgctactc gtaatgcctg cttcatcata tctcccttat ctggaccgat ggcccacgat
     3121 aatttagcag aatgttaaca aactaaaata cacaaaacta ccagaacccc gtggcaaccg
     3181 gtgcctgatg ttgaccgtcc gaacagtcaa catcgcgatt acggtttgaa gtccaatggc
     3241 gcgtttttga acacttcata tactgcctgg cttggtggtt tcgggatctt cgcaagttta
     3301 gctgctgcca acgcagcctg acaaagtgcg ggatcgccac cttcaggttt gatatccagt
     3361 aacataccat cgggtgccag ttttatgcgc agcgtacagg ttttgcctgc ataggacgat
     3421 gcgtcataga acttactttc gatagcagat ttaatctgcc cggcatagtt attgatatcg
     3481 gcccctgatg cgccattgtt tttagtatta ccactcccgg caggcgaagc attgttccct
     3541 ttcgcccctc cccccgtttt cggtgcattc ttaccagagc ttagctcacc gaaaatatca
     3601 tctgcctctg cggcagcttt tgctgcagcg gctttttcag ctgctgcttt tgccgctgcc
     3661 gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc tgccttatca
     3721 gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc cgctttctta
     3781 tcagctgcag ccttttcagc agccgctttc ttctcagctt ctgccttggc tttttcagca
     3841 gcttcagttg ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc
     3901 ttcttcagtg ccgcagcggc tacctcggct tttttctgcg cttcggctgc ggctttggcg
     3961 gcttctgctt ctgctttttt cttcgcgtct gcagccgctt tcttcgctgc ttcttccgca
     4021 gctttagcat ctgccgccgc tttcgctgcc gccacttcag cttgcttctg ctttaactcg
     4081 gcctgttttg cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc
     4141 tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc
     4201 tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc ctggctttgc
     4261 atgcgtttgt actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa
     4321 ccaccgccgc ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgcc
     4381 gcaaataaga tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt
     4441 tgttcggttg cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct
     4501 ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac
     4561 acccgcacta tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc
     4621 gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac
     4681 cacctgctct ggtggtaaac gctccaggcg atctttctca accaccacgg tgtactgacc
     4741 aataccagac acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc
     4801 agtagcgtct ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa
     4861 gatcagcaac agcaccagca gtacgtccag caacggaaca atgttgattt cggacttgag
     4921 atcgcgacga cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac
     4981 ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag
     5041 ttccagtttg tttacgcgct ggttgagacg gttataagcc attaccgccg gaattgcggc
     5101 gaacagacca attgccgtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag
     5161 tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc
     5221 aaacagacca atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc
     5281 cagattttca agttcacggt tcatggagat acgcatagca cgcgacgccc cttccactac
     5341 ggcttccggc gcatggctat tggcacgatg caggcgcaca aactctttga acccgctgta
     5401 aaagatttgt tccgaaccag tcagattatc ccgtttcccc tgactctctt gatagaggcg
     5461 agagagttcg attccagacc agaatttatc ttcaaacgct tcggcttcac gcgccgctgc
     5521 gttaagaata cgggttcgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa
     5581 aatcaacatg ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat
     5641 gtcagtcact gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg
     5701 agtgggtcaa cgcaaacaac cagaacctct gcttcattca gcaaagtatt ctcggcgttg
     5761 acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt
     5821 tcgagcatat cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca
     5881 aaggcaacgc gttcagccat cagcgcctgc tgactgaagt gatgatgacg cagcatctct
     5941 gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg
     6001 gtatcttcat agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc
     6061 ggtaatgcaa caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt
     6121 tgggaatgag ggcaagttaa gggcgcgtaa aaatttatgg gcctagagcg gcccataatt
     6181 ttttaaagag aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat
     6241 cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga aagcccacgc
     6301 cgtgaatcac accagcacag acggcccaca tcagcaataa accatgccag atttccagtt
     6361 cactggtctt cgcggcaaaa cgagatgggt cccaaaacat acatcctgct aacagaagcg
     6421 ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggtcgcga
     6481 taatcttact catcagatgt cttcttgacc ggctttgcct gattcgacgt gttcaagcgc
     6541 cagcgcggtg attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata
     6601 ccacattttt agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg
     6661 gtgatacgac cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc
     6721 agaaccaccg caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac
     6781 attgtcagac ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata
     6841 ccggctgtca ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct
     6901 ttatccatac gtgcagtcag gatggtcagc agcagcagaa ccacacccag tgccggaata
     6961 gcccacagaa ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact
     7021 tctttattca gtgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca
     7081 tcgataccgt acatcaccca tacgccagcc agtgcgaaac agaccagtgt caccagcgca
     7141 gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg catttgcaga
     7201 taggttgcgc cctgagtaat gatcatccct acgctcacca cgcctgccag caggccgaac
     7261 gggttaagca actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg
     7321 aacggtacgc cctgcaacag gttaccgaac gctacaccaa ttaccagcgg cggaacgaag
     7381 ctaccaatga agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag
     7441 cggtagtcaa aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca
     7501 tagaagccgg agaacgcagc ggcatagacc atcggccagg cagcaaagag tgcgccgccc
     7561 gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg caatggagtt aatcataatt
     7621 cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa
     7681 ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca gcagccacca gataaaacgc
     7741 aatacttcat aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg
     7801 gaagactgct caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac
     7861 ttgaacatta agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc
     7921 attgagaaga tgagatcgcc tgcggtcagt gacgaattcg ccacagctgt cggcagcact
     7981 tcaccgatag cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca
     8041 atccacggca gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt ctcgccaatg
     8101 cggttgcgga tgacactcca gaaagagagc gcgatgattg ccagcagcag gaagccacac
     8161 gccaccatga tacggaacgc aaagtacagc ggcgctacac gcgggatgga gtctttggtt
     8221 gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc
     8281 agaccgtaac cgaggtcttt cttcatgcta ttgaactggt cacgaaccgt ctggtcggta
     8341 gaaccagaac gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct
     8401 tcatgctgca ccatcagctc tttcaggccg ataaccgggg tatccacgga acgcgttgca
     8461 atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc ttcctgatca
     8521 ggaatgccga acaaagtaaa ggcagcaggc gcaggttgcg tttcccactc ggcttcaata
     8581 gcagccagtt tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca
     8641 ataacagaca gaacagcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg
     8701 aaggcgaagt cacgaccttt cagcatatac catgcgctga taccgaggat gaacatcgcg
     8761 ccagtcacat aaccagacgc tacagtgtga acgaatttca cctgagcaac cgggttaagc
     8821 accagctcgg agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg
     8881 attgggtttt gcatccagcc gttcgcaacc agaatccaca gtgcggacag gtttgaaccg
     8941 agcgccacca gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag
     9001 aagaacagac ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc
     9061 ggcgcaccga agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg
     9121 aactccatgg tcagaccggt agccacaccc agagcgaagt tgataccaaa caacttgccc
     9181 cagaacttgg tcatatcttt ataaatctgt ttgccggaga ggacgtagac cgtttccata
     9241 atggccagga ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg
     9301 gtcaaggcaa actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca
     9361 tcgcatgaag actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataga
     9421 aatttgtttg aacactttca tcataccatg tgaatgcaca caaaaacggc gtaaagaaag
     9481 gttgcaaaca tgttaataaa aactcaaatt gatcccacgt atatattacg ccgcaaaatc
     9541 cttacaataa acaggttttt gttgaagcaa atttgcattt ttcgacggtg atcaatttat
     9601 agctaaattg ccgcctttca gccaatttga tcgagaacaa tttatctctt tttgatgccc
     9661 atttccaaga cttatacatt gataaatatc aatttctaca aattatgtta atcatgtttc
     9721 aacaaggtaa ctttagtgat atttatgttt aaaatgtgtg aattataaga ctgaaattag
     9781 tttcggttgg caattaaaga taaaaagaga cgaagcaaaa acagcacggt tgaccttaat
     9841 tctttttttg gtcactggct aatacgacgc agagtttcag gattattaat aaaggcataa
     9901 atctcccccc cgccgatatt aacatttaat taacttataa ttatattata attaactcac
     9961 ccgtggttat tattttgtta aaaataaagc cacatatacg tagcttgaga gtgaagataa
    10021 acacaaaatt acctgatgcg ctacgcttgc cagtgtctac atcatctctg caatatattg
    10081 aatttgcctg cttttgtaga ccgaataaag cgttcatgcc gtatccggca tgaacaaagc
    10141 gccctttgtc aacaatctgg ccgcgcatat gtggcttcgg tttgattgct attcaggcaa
    10201 gccggtaact gaacgtccgt gactggccgg gtaaaaaagg ccccgataga tttgaacctt
    10261 gattttcctc ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa atcacctcgc
    10321 gactgaaagc aacagtcgca tcacaggttg ctgattcagc cggattaaac agccgtaaaa
    10381 tcacttcttg tcggtcttca gctttcttaa gtgcgcttat caggcatccc actgggggca
    10441 ttttcaacaa actataactt tccggcacgt tgaatccggc tttgttgagc ttcatcgcat
    10501 cccatgggat tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc tgctgagcta
    10561 caccagcggc ggttggcgta ccggtataac tcaataaact taggcgacaa gaaagcagac
    10621 cacgtagttg tgagtccggg actggcattt taattcccga aggccgccta ggccttaaaa
    10681 gcagatcttc tttccccagt aagcccacgc cgcgcagcaa cgtaatggca aaggttttct
    10741 tctcttcacc gatgacttca aattcacgta gcccttcgct aaagacagcc atgccgttac
    10801 gcccttcctg taaggctgca tagttgagca tattccatac cggaaccggc gcttctttcc
    10861 agctttcttg ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt agcgaaccaa
    10921 actgggtatc tgccagaaca ctatcggtgt taaaagatgt agggatcagg acgcgaaggc
    10981 gatgatcgtc agcctggtta tcaaggttga tatccacatc aatgcgcctg ttgttatgac
    11041 taagagtgac aaccaactcg acatctactc tgccagtgga ttgtcttgcg ctgcgttgtt
    11101 gcagattacg cggcaccgcc atttcatagc ggataacagc cctgctctgc caggcctcat
    11161 gaataatctc gcattgcggt ttcgcattcg ccgaagtaat tacccactct tcttttgcgg
    11221 gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaac acccggtcat
    11281 agcgcacacc gctatcttta tctaccagtt gtagcgttcc atcctcattg agcgcaattt
    11341 gccagaaagc attttccagt atcccttcag cgtcactttt tgccgcaatc acgttgccag
    11401 gctgattcgc ttcgatataa agcgtgcgat agcccataga agggacaatc tggttgatct
    11461 gtatatcaaa ctccataaag ggatcgtaat taccgtaatg aactatttgc cgatcgatta
    11521 ggcctggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga ccgcgatcgt
    11581 ctcgtagatt aaactggctg gcgcgcagcc gcacagtggt gttgatcact tcttcccgcg
    11641 gccagggcat caggttaaac aggacgagtt tgtcggcgtc gctctgcggc atgttgtcgg
    11701 cgattttacg catatagaaa cgcagcagat tatccgccat gtcttcagcc agttcgaagc
    11761 gggcgacgat ttcgcgatga actttgtcac gacagcagca gccgatgctg tcgtgggcat
    11821 gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat ccaaaaccca
    11881 acgtcctggc cagcgttgcc agcggttcca gcagattaac aatcttattt tcaatacgtg
    11941 cgtgggcaac tttgatatcc atgcgcgtgg aaccgatggt gcgatgcacg cgcatatatt
    12001 tgccatcaat aaattcccct ttcagggttg ccagattatc tcgctgcgct tcgatcttct
    12061 caaatacctc ttcaaagcgg ctcatcacaa actttcgttg aggataaatt tcgcgcagtt
    12121 tctccatcac ttcgaagata ttttgctgca atggcatctg atcatgccca ttcggcagca
    12181 aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg aggcgtttgc
    12241 gtaatccgtt ttcgtccgca ggtaagtact taccgatggc ataacccaac ggcatcacct
    12301 gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta tccgtaccgt
    12361 ggcgctcaga acatccacgc cagaacatgg tgcaggtaat gccaaatcca ttgtagatat
    12421 gcggcagttg cccggacatg ccaaaggaat cgggtaaata acctattttc atcggctcgc
    12481 caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat tccgcagaaa
    12541 caatcgtggt atcggtctgg gtataccagg ggccgataat caacttgccg gcttctacca
    12601 gtttcttcaa gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg aggatcgccg
    12661 tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg cacaggatct
    12721 cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac cactcccgat
    12781 cccagtgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc gtttcctttt
    12841 tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt gacgccgcca
    12901 gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa accagccaat
    12961 tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga aaatgccagc
    13021 accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta ccatagagcc
    13081 cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga tcgccccttc
    13141 ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt caaactcttt
    13201 aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa ccattttgac
    13261 ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca ggcagaatgc
    13321 ataagcggct ttattcactg gccctccaag gtcaaaggaa cacataaaac cgagaatggc
    13381 acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa gcgagttatt
    13441 gatccaggcg acaggttccc ccaccacaaa cagcatcaga ctgcccgctc ccaatgtacc
    13501 gagcaccggg tagagataaa aagtcaggaa gccattgaat ttactgctaa gacgcaagtg
    13561 atttttcatc cagcgtatca agtaaccggc tatcaatccg ccaacgaccg cgccgagaaa
    13621 cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta acgccggttt
    13681 atctgccagc gaataggcgg tataggctgc cagcaccggt accatcaaaa ttccgagcag
    13741 cccgccgccc agtttgcggt acatccacag ccaggagttt tcttcattaa acagatcttg
    13801 tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtccccc ccgcgacaat
    13861 caacggtacg gcaaaagaga tcccgctcaa cagtgcctgt ttcagctccg ttttgacgct
    13921 tttcaccggt tgcgtatctt gctgtaaggt acgcgtctca tcgccacgct cgagggtaag
    13981 cgattgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca cagaaagcgc
    14041 aggaatgccg ttaaaacgct cactctcctt gatggcgact tcagccgcaa aaatacaggc
    14101 agttgcacta ttgagttgat ccgccgttaa acgcccttca ataccgttag caccttgctt
    14161 ttcaacgtag acattcacgc cgagtttgcg tccggctttt tccagatact ccgcagccat
    14221 gtaggtgtga gcaatacccg ccgggcaggc cgttacgcaa acgatagttg gcgcgttgga
    14281 aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca attcatcagg
    14341 cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gccaggcgcg tggtcagcgc
    14401 tgtcagcagt tgcatgtgcg tagtacccgc ttcattgagg ggaatcgcca gcaacaccac
    14461 taaatcaacc gcttccgggc catccacgcc ttcccactga agcggctcgc tgagtgtggc
    14521 gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag ccaacccttc
    14581 acttaaggcg gtcgggccaa ggctttcacg gcgatacact tctttcagaa attgctcagt
    14641 actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtgaatgg cctcttcacg
    14701 gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg tcaggttcat
    14761 catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgacaaa acaataccat
    14821 tgcgtaatca atttcacttg attgaaatta tttgcatttt atttgtattg tttacaggat
    14881 gagtttatcg ggcataatgg ctaaattcgt attaatgaga tgctaaataa tgggacacaa
    14941 acccttatac cgacagattg ctgatcgcat tcgtgagcaa attgcgcgtg gcgagcttaa
    15001 acctggcgat gcgttaccta ccgaatcggc attacaaaca gagtttggcg tcagccgggt
    15061 tacggtgcgt caggctttgc gccagttagt cgagcaacag atcctcgaaa gcattcaggg
    15121 cagcgggact tacgtcaaag aagagcgggt caattacgat atttttcagt taaccagttt
    15181 tgatgaaaaa ctgtcggacc gtcacgtcga tacacacagt gaagttctga tatttgaagt
    15241 gattccggct gacgattttc ttcagcaaca gctacaaatc accgtgcagg atcgtgtttg
    15301 gcatgtgaag cgcgtgcgct atcgcaagca aaagccaatg gcgctggaag aaacctggat
    15361 gccgcttgcg ttgttcccgg atctcacctg gcaggtcatg gaaaattcga aatatcactt
    15421 tattgaagaa gtgaagaaga tggttatcga tcgtagcgaa caggaaatca ttcctctgat
    15481 gccaaccgaa gagatgagcc gtctgcttaa tatcagccag acaaagccga ttcttgaaaa
    15541 agtatcgcgt ggatatttag ttgatggtcg ggtgtttgaa tatagccgca acgcttttaa
    15601 taccgatgac tataaattta ccctgatagc gcagcgaaaa tcatcgcgat aagcacaaaa
    15661 aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat tcctgtagag
    15721 ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt ttcagtgctt
    15781 caccgatatc cgccaggctg cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt
    15841 tctcatccgc agtccctttc ccaccggcaa tgatcgcacc cgcgtggccc atacgtttgc
    15901 ctttcggcgc agtcacacca gcgatgtaac ccacaactgg cttggtaaca tgctctttga
    15961 tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatc acgatcgctt
    16021 cggtctgcgg atctttttcg aacatttcga gaatgtcgat aaagttagag ccagggatcg
    16081 ggtcaccgcc gataccgaca caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa
    16141 ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc ggtttgtgaa
    16201 tgtgacccgg ctggatacca attttgcatt cacccggggt gataacgcct gggcagttcg
    16261 ggccgatcat acgaacgcct gcttcgtcca gtttcacttt cacggtcagc atatccagcg
    16321 tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcgata gcttccagaa
    16381 tggagtcttt gcagaacggt gctggtacgt aaataacaga cgcggaagcg ccagtagcag
    16441 caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagtg ccgcctttac
    16501 ctggagttac gccaccaacc attttagtgc cgtatgcaat ggcctgttct gagtggaaag
    16561 tcccctggct accggtaaag ccctggcaga taaccttggt gtttttatca attaaaatgg
    16621 acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca gaccttttgc
    16681 tgcaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc
    16741 ttccagacgt accacgaccg gtacgttaac acccacttcc gctaccgcgc cgatgatacc
    16801 gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa cggctttcac
    16861 tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc
    16921 gccaacgtcc aggaagttag ccggttcgcc gccgtgcagt ttaactatgt ccatcgtacc
    16981 catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg caacgtagtt
    17041 cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt cacgcatttc
    17101 gcgcagatca ggctggcgga acagggcgtt gccatcagcg cccagtttgc cgtcgaggca
    17161 aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcagcg ccaggtcgcg
    17221 ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa ccagtttacc
    17281 ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg
    17341 atcaagcgca actttatgga tcaggtgcgg agtttcttcc gccacttttt cgatttccac
    17401 gccgccttcg gtggaggcca taaagaccac gcgacgggaa ctacggtcta caacggcacc
    17461 gagatacagc tctttagcga tatcggtcgc cgcttcaacc agaatctggt taaccggttg
    17521 gccattggca tctgtttgat acgttaccag acgcttgccc agccagtttt ctgcaaaagc
    17581 acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc ggccaccagc
    17641 gtgaacctga catttcacta cccacggacc ggcaccgatt tttgaagcgg cttcttctgc
    17701 ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc gggcaaacag
    17761 ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag taatcgttat
    17821 cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt gaagcttaaa
    17881 ctactacacg tccagcagca gacgcgtcgg ctcttccagt aactctttga tcgttaccag
    17941 gaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg ccaggtacat
    18001 catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga tagcgtgcat
    18061 acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg aaccgaacac
    18121 accaccgttg gtgatggtga agttaccacc ggtcagatct tcaaccgtca gcttgccatc
    18181 acggcctttg actgccagct ctttgatttt cttctcgatg tctgccatgc cgagggtatc
    18241 gacatcacgc agaaccggcg tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc
    18301 gaaatagttg tggtaaacca cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg
    18361 tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac ggatgccgtg
    18421 gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct tcatgttgac
    18481 ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac gctctgccac
    18541 acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca gagccggttg
    18601 cgccgccgga gcagccgctg ccggagcaga ctctttcgcc ggggctttcg ccagatgttt
    18661 ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg cgctggcgtc
    18721 gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt tgttttgctc
    18781 ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag atttggcgct
    18841 ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac gagacgttac
    18901 cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg ccggtacttc
    18961 cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga ctgcgtcgcc
    19021 gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca ggtcagggac
    19081 cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta
    19141 accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg agaggcggag
    19201 gccgggcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc
    19261 tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac aaaatccttg
    19321 acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg gtagagttgc
    19381 tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc cagcaggtcg
    19441 taataaacct taccagaaca catcactacg cgcttcacgc ccttcggatc aagctcgtcg
    19501 atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc
    19561 agcggatgac gcagcaggga tttcggcgac atcacgacca gcggacgacg catcccgcgc
    19621 agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggtac gcaaacctgc
    19681 atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga gtgctccggc
    19741 ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg gccccatttc
    19801 tgctcgccag aggagatgaa ctggtcgata accacctgcg caccgttggc gaagtcaccg
    19861 aactgcgctt cccagatggt cagggtgcgt ggttctgcgg tggcataacc atattcaaac
    19921 gccagcacgg cttcttctga cagtacggag tcccagacac ggaacgcgcc ctgaccgtta
    19981 tggatgtgtt gcagcggcgt gtaagtggaa ccgttagact ggttgtggat caccgcgtgg
    20041 cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg aatgccttca
    20101 tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag tttctcaccg
    20161 gctgccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat ttcaactgct
    20221 tctggtaccg tactgatacg tttagccagt tcctgcaggc gcttcatctc aactttgttc
    20281 gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc
    20341 atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta
    20401 accatctcgg tggcatcttc cagcgtcgcc actttttcct gctccagctt gtcagcgtag
    20461 attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagtggctg ggttgcgctc
    20521 ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcata ccaggtcgat gaagacatca
    20581 cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac ggcttccgga
    20641 tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc agtacagtac
    20701 ggtgtagaac gggcatccag cgggttagag gtggtgaaac caacctggtt gttgataacg
    20761 atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag agtttcctga
    20821 accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag cactttgttg
    20881 ctgttcggct catcaagtct gtccagacgg gcacgaacag agccgataac aaccgggctt
    20941 acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc
    21001 tggaagtcag acgagaagcc catgtggtat ttcacgtcgc ctgtgccgag gtgttcttta
    21061 tgtttaccgg cgaactcgtc gaacaagtct tgcggttttt tacccagcac gttcaccagc
    21121 acgttcagac gaccacggtg cgccatcccg agaaccactt cgcgggtgcc gctgttgcca
    21181 gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc cagcgagaag
    21241 cgttttgcgc cagggaattt tgcgccgagg taacgctcaa ggccttcagc ggcggtcagt
    21301 tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc
    21361 tgttggatcc agcgtttttc ttcggtgctg gtgatgtgca tatactcggc accaatcggg
    21421 ccgcagtagg tttgcttgag ggcggaaata agctcgccga gtttcatggt ttctttgccg
    21481 ctggcaaatg aaccgacgtt gaaggtttcc tggaagtctg cttcggtcag atcgtggaaa
    21541 gacggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc tagattcgca
    21601 tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac ctgcttcaca
    21661 ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc caggcggcgg
    21721 aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc aggtaactgc
    21781 tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa gtcttcatag
    21841 agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa ccaggctttc
    21901 aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact tcgccgtgga
    21961 tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg tctacgtagt
    22021 ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt aaagatttcc
    22081 tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctgcgggta atgacttgta
    22141 ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcaac gcttgcgcgt
    22201 cttatcaggc ctacagttta cgcattacgt tgcaacaaca tcgacttgat atggccgatg
    22261 gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat gctgtgacag
    22321 cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg
    22381 ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa cttatcggga
    22441 ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca ttcatacagc
    22501 ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg cggattttgt
    22561 ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg tcccatgtct
    22621 accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat cttcttgccc
    22681 ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc gttcatgttc
    22741 agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag gctgggatct
    22801 ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc
    22861 agggtgtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg ataaattgaa
    22921 aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc
    22981 gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga ctcaggcaga
    23041 tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa gcggctatgt
    23101 gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc cgtttccatc
    23161 aggttatcca gttccaggca ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc
    23221 agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc tttcgccatc
    23281 gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag cgctttacgg
    23341 atcgccaccg gatcttcacc gttacgatta ttgttccacc ggttcaggcg atccagagac
    23401 gcttcaacat cagactcgct ggcatcgtgc agtgcgccct gctcggcgat agactcttgc
    23461 agatgcagac ctgccgcgcg accaaagacc accaggtcca gcagcgagtt gccgcccaga
    23521 cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag tcccggaaca
    23581 accacatctt cgcctttctc attcacagtc agcgcctgac cggtaacttt ggtcggaata
    23641 ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac cggatcgacg
    23701 tgagcgaagg tacgggaaag ctccaggata cccggcagac gggattcgag aacttcttta
    23761 cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct
    23821 tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag gtctttggcg
    23881 ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata accgccttca
    23941 ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc
    24001 cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac accgtcgcca
    24061 gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgctccgcc agtcgccagc
    24121 acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag tgcggtacaa
    24181 cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc ggagaaaatg
    24241 gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg accggtacgg
    24301 tcagcagccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga ctgaccgcca
    24361 aacggacgtt gatagatacg accatcatcg agacgcgaga acggcaggcc catgtgttcg
    24421 agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc ctggtcaccg
    24481 atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc ttcatgggta
    24541 ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag
    24601 actttagaga gcagggcaca ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata
    24661 cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa tttcatcaca
    24721 caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg acaatcacca
    24781 gttgcagcat caggcgcaag gccagcggtt taacgtagtc ggtcaacacc tgccacatgc
    24841 cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact ttggtgaacg
    24901 cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg gcgaaaaaac
    24961 cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg aggatgaaat
    25021 catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga ctcctgcgag
    25081 aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac ccgcttcgaa
    25141 tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga cgacgtgata
    25201 cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc ccataatcgc
    25261 ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc actgcaggat
    25321 gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg ctatcgccgt
    25381 gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt tcacatttct
    25441 tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg ggcacagagg
    25501 ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct tgcgggtcag
    25561 acagcgtcct ttccataact gcgcgtcatg caaaacactg cttccagatg cgaaaacgac
    25621 acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg cggcagtata
    25681 ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt tatcctgaac
    25741 agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat ttgacaatca
    25801 ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa agttatgaag
    25861 tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc aattcattcg
    25921 ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact tgattcgcca
    25981 tttattcgtc atcaatggat tctttacctg caagcgccca gagctctgta cccaggtttt
    26041 cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt tgatgtgcga
    26101 aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc ttaaatggct
    26161 gatacaaaag caaaactcaa cctcaacggg gacacagctg ttgaactgga tgtgctgaaa
    26221 ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg tgtgttcacc
    26281 tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt tattgatggt
    26341 gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac cgattctaac
    26401 tacctagaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga acagtatgac
    26461 gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac ccgtctgttc
    26521 cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac cggcgcgctg
    26581 gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga aattgccgcg
    26641 ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta ttccattggt
    26701 cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct gaatatgatg
    26761 ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc tatggaccgt
    26821 attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt gcgtaccgct
    26881 ggctcttcgg gggcgaaccc gtttgcctgt atcgcagcag gtattgcttc actgtgggga
    26941 cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt
    27001 aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg
    27061 ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc
    27121 tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt ggctatggag
    27181 ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta cccgaacgtc
    27241 gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat gttcaccgtc
    27301 attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat gcacagttac
    27361 ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg cgactttaaa
    27421 agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc cgccgttcaa
    27481 atggcgggtt aatttttata tgcctaaaca caaaaaattg taaaaataaa atccactatc
    27541 ggacattcat atattctaaa aaaaaataca tcggtataaa ttataaacaa atagatgatt
    27601 tttaatcatc tatcttcctc tcagttacat gaataaacca ccaaatatag cgcaccccta
    27661 taattcccaa tactttattc atcaaacatt taattcatac catcaattaa ttacttttac
    27721 atttaataaa attaataata tttttaagaa aaactaaaaa cattcgacaa tcaaataaga
    27781 tattcttgca ccgatatact tttttctttt ctgttaaagg actcagttat gtttaaagga
    27841 caaaaaacat tggccgcact ggccgtatct ctgctgttca cagcacctgt ttatgcggct
    27901 gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata
    27961 catcaggatg atattgataa agaggttgaa ctcggtcagg tgaccaccag ccacattaat
    28021 cagtcacatc acagcgatgc cgttgctgtc gacctgctct tagtcaactg tgatctggaa
    28081 aactccagca acggttccgg tggcaagatt tctaaagttg cagtcacctt tgatagctca
    28141 gcgaaaacca ccggcgcaga tccgattctc aacaacacca gcacgggtga agccaccggc
    28201 gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg ttttaggcac cgctactcca
    28261 gatatcgacc tggctccgac ctccagcgaa caaacgctga atttctttgc ctggatggaa
    28321 caaattgatc aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg
    28381 ctggattata aataagtatt actttggcgg cgctatcgcc gcctttcata aaatatttat
    28441 caggtatgga caccgtgaat atttatcgac tctcttttgt atcctgcctg gtcgtggcga
    28501 tgccttgcgc attggcggtc gaattcaatc tgaatgttct cgataaatca atgcgcgacc
    28561 gcattgatat ttcattatta aaggaaaaag gagtcattgc tcccggtgag tattttgtta
    28621 gcgttgcggt aaataataac caaatcagta acgagcaaaa gattaactgg cacaaaaatg
    28681 acgataaaac cattccgtgc atcaatgatt tactggtcga taaatttggc ttaaaacctg
    28741 aagtccgtca gtcgttacca ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa
    28801 tgctcttcaa tttcgatcaa gccaatcagc aactgaatat tagtattccg caagcctggc
    28861 tggcgtggca ctcagaaaac tggaccccac cctccacatg gaaagaaggt gtcgccggtg
    28921 tcctgatgga ttacaacttg tttgccagca gctaccgccc acaggatggc agcagcagca
    28981 ctaatcttaa cgcctatgga acagccggaa tcaacacggg ggcatggcgc ttacgcagtg
    29041 attatcagtt gaatcatact gatagcgatg ataaccatga acagtcaggc gaaatatcac
    29101 gtacctatct ttttcgcccg ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg
    29161 attttagtcc caatattttc gacggttttt cttataccgg cgcggcactg gcaagtgacg
    29221 atcgaatgtt gccatgggaa ctacgcggct acgccccaca aattagcggt attgcacaga
    29281 ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac
    29341 caggcccatt tatcattgac gaccttaatc agtctgttca gggcacactg gatgtcaaag
    29401 tgacggaaga agatggtcgg gtgaacaatt tccaggtttc agcagcatcg acgcccttcc
    29461 tgactcgtca gggacaggtt cgctataaac tggccgcggg tcagccacgg ccctccatgt
    29521 cacatcaaac tgaaaatgaa acctttttta gcaatgaagt ttcctggggg atgctgtcaa
    29581 acacctcgct gtacagcggc ctgctgcttt ctggtgatga ctaccattct gccgcaatgg
    29641 gtattgggca aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta
    29701 gccattttga tactcagcag gacgagcggg gcttaagcta ccgttttaat tacagcaaac
    29761 aagtggatgc cactaacagc acgatttccc tcgccgctta tcgtttctcc gatcgtcatt
    29821 ttcacagcta cgccaactat ctggatcaca aatacaacga cagcgatgcg caggacgaaa
    29881 aacagacgat cagcttatct gtgggccaac cgattacccc actaaacctc aatctttacg
    29941 ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg
    30001 caggttttaa tgttgatatt ggtgactgga gagatatctc tatttctacg tcattcaata
    30061 caacccatta cgaagataaa gatcgcgaca accagattta cctgtcgatt tcgctcccct
    30121 tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc
    30181 gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc
    30241 aatccgaccg tcctgacaat ggagcccagg tgagcggtaa ctatcagcac ctgagttcag
    30301 cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca
    30361 gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca
    30421 ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca
    30481 atctcgacta caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc
    30541 cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg
    30601 ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg
    30661 gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg
    30721 atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct
    30781 ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt
    30841 gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc
    30901 attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc
    30961 cagtctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa
    31021 tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta
    31081 ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc
    31141 gatacaacgt ctggaaccga aagcaacaac acaggtacgg atagtaaagc aagcctcgac
    31201 cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc
    31261 cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa
    31321 ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt
    31381 acaggtcagc cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac
    31441 cattgcctac ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt
    31501 tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct
    31561 cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg
    31621 ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc
    31681 tgtctgttat ttctgtcgtt gaagtctgcg cttgccctga actgctactt tggttcatcc
    31741 ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa
    31801 ctcggcgata agatctggga atctgacgat attaaaataa gatctgggaa tctgacgata
    31861 ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ctttgaaagc gaacatgtct
    31921 acgtatgggt aaacccctac ccgggggtgc aggatcgtta ttatcaactc ggagtaactt
    31981 acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact aaccagtgta
    32041 tcgacagtaa gaacattgac atttataccc ctgatgggat ggcagaacaa aatttgctcg
    32101 ggtgatcccg ctaatattca catgtcgcga acctttctcg ctcgcatgcg gttatacgtc
    32161 aaaatacgag aaatgccgcc gcatgattat caaagtacgc ttagcgacta tatcgtcgtg
    32221 caatttgacg gtgccggtag cgttaacgaa gaccctactg cccaaaacct gaaatatcat
    32281 attactggtc tggaaaacat tcgcgtgctg gattgcagcg tcaatttttc catttcaccg
    32341 gaaacacaga tgattgattt tggtaaattt aatttgctgg atattcgtcg ccacacaatg
    32401 tcgaaaacat tcagtattaa gacgacaaaa tcacaaaatg accaatgcac cgacggattt
    32461 aaagtcagtt cctcctttta taccgaagaa acgttggttg aagaagataa agcattgttg
    32521 attggtaatg gtctgaagct acgtttgctg gatgaaaacg cctcgcctta taccttcaat
    32581 aaatatgctg aatacgccga tttcaccagc gacatgttgg tctacgaaaa aacctataca
    32641 gctgaacttt cgtccattgc aggtaccccc atcgaggctg ggcccttcga tacagtggtg
    32701 ctttttaaaa ttaactataa ctgatttgcc atgatgccct ttgcagccgg aaagggcatc
    32761 tctttcagaa aattgctgat tttccccttt tccttgattg ctgttatttt ttcctagccc
    32821 ggctttcttt atgctgtatc gatacaccaa agagaaaata atgagggagc gaaggatgcc
    32881 agttttgcag tggggaatgt tatgtgtgct atcacttctc ctttctattg gtttcctcgc
    32941 ggtacatctc ccggcggcgc tattacttgg gccgatgatc gccgggatca tcttcagtat
    33001 gcgcggaatt actctgcaac ttccccgctc cgcttttctc gccgcgcagg ccattcttgg
    33061 ctgcatgatt gcgcaaaacc tcactggttc gattctcacc acgctggccg ccaactggcc
    33121 gatcgtgctt gctattttac tggtgacgct gctttccagc gccatcgtgg gttggttatt
    33181 ggtgcgctat agctcactgc ctggaaatac cggtgcctgg ggttcttctc caggcggcgc
    33241 agcggcaatg gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat
    33301 gcaatatctg cgagtgctgt tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct
    33361 gggtgataac gctgaagcgg ttaaccagca aatcgtctgg ttcccgccag tgagcattaa
    33421 tctcctgctt acgattttgc tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct
    33481 tccttccggc acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct
    33541 catcaccatc gaactaccgg aatggctgct ggcgatggcg tatatggcaa ttggctggcg
    33601 gattggtctt ggtttcgata aacaaatctt actgcgggca ctacgcccgc taccgcaaat
    33661 cctgctgtcg atttttgctc tgctggctat ttgtgcaggt atggcgtggg gactgacccg
    33721 gtttatgcat attgatttta tgactgccta cctcgccacc agtcccggcg ggcttgatac
    33781 cgtggcggtc atcgccgcag ggagcaatgc cgatatggcg cttattatgg cgatgcaaac
    33841 cctgcgcctg ttcagtattt tgctgacggg gccagccatt gcgcggttta tttcaaccta
    33901 tgcgccgaag cggtcggcct agtgctggca gcctggacac cagtaaaacg ggcgcgagga
    33961 caacgtggtt ttctcaatga tgccgccaca gcgttcgcac agttcgccat cacgatgaaa
    34021 aaccttaaag cgaaacagcg ccccatgatg cttattctca tccacctgcc cccgcgtagc
    34081 gtaagaaaac cgaggaatct ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt
    34141 gagatctttc gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa
    34201 ataattgcca agcccagcca gaaacgcctg atcgagcagt aatcctgcaa actgacggtt
    34261 acgaaagcgc ggcgacaata accgttcctt taccacctcc ggcgtcagat tcggatccag
    34321 cacatcggga ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat
    34381 ctcaatatcc gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg
    34441 caatactcgc gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag
    34501 ctgattatgg ctgtagagcg ttaaaccgtt gggaaaatga gttaacaacg ccttaccacg
    34561 cgtttccaca tgggtaacgt gttgaccgat aagttgtgat tgataagttt ttaactgcgg
    34621 gaaggcaaac cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc
    34681 tgcacggcgg atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaag
    34741 cggggttatg ctgcgacaac aatccccttt ttggcaaatg tagctcgtaa tcggcgggcg
    34801 aacgccagag cgtgctcgcc gtcgccatgc aggcagaccg tttgcgccgc gaccgttgcc
    34861 cattcgccgg tgatactttt gactctgccg tgttgcacca tctccagcgt ttgcgccaac
    34921 gcctgttctt cgttttcaat caacgcgcct ggctgacttc gcggcaccag cgagccgtca
    34981 gcctgataac cgcgatcggc aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct
    35041 gcacgaatca gctcgcttcc tgccagccca acgagaacca atgccggatc gcaagcgtat
    35101 accgctctgg cgatggcgtc tgccagttgt gcttctttcg ccgcctggtt gtacaacatg
    35161 ccgtgcggtt tgacatgacg cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg
    35221 ccaatttgat acagcgtctg ggcgtaaacg gtttccggcg gcagctgcat ggcgctgcga
    35281 ccaaaatttt ccctgtcggg aaaactcggg tgagcgccaa tcgcgacacc attttttatt
    35341 gcttcacgca cgcaagcctg catggtttgc gcatcgcctg catgaaatcc acaagcaata
    35401 ttggcagagg aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg
    35461 gcgttcaggt caattttcat tgtgcagccg ccacgctaat tgttcaaaat aacgttgctg
    35521 atcttgccgc gctttcagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc
    35581 gagcggaatt tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgtggata
    35641 accaccggtg gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gcggcacctg
    35701 caccacgccc ggtaacaaac cgtgagataa cagttcgcga tcggtgatgc gttttaaaat
    35761 ttgcccctgt aaacgatagc ccattcggtt actttgcgag ctaagctgcc agggcgaacg
    35821 ccagaatgca tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg
    35881 aatgcggttg ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcga
    35941 tttgccaatc gggagtcagt caccatcctt cagtaaacgg ccttccagcc cgccaatccc
    36001 cactttgaga tcggtgctgc atgaccccat taccggcgga acatcaatac cacccgcgac
    36061 cgccagataa ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt
    36121 catcggcaat cgccagccgg tccagacggc gttatcatcc aaccgcgctt cgcaacctgc
    36181 acccgtcagg gcaaaccacc catcagtttc gaactcaact gttaactgac cgagcgtgat
    36241 ctccagcgcg ggggcattgg cgtcattacc caccagcagg ttagcaatgc gcagcgcagg
    36301 catatccagt gcgccacagt ggctgatacc cgactggcaa aaaccgtgac gaccgccatc
    36361 ctgcacagtg gtgtacatgc ccgcacgaat aatcttcagc atattccctc cttctgcggt
    36421 acaaatcgca cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac
    36481 aggctgagtg aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca
    36541 ccagtctgcg gcccgccgat cccgacagaa cctgccggaa cgagcaagcg cggttcagcg
    36601 cgccgtggcg tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa
    36661 cctaaaaacc agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc
    36721 aacccgcaat acgccgcgac caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc
    36781 ggaatttcaa taaagcgaga ctcaggttcc agggcctcgc tctcctccca ccagcgttgc
    36841 aaacgctcta tggcatccag cgccagcgac tcaggattac gcaaaatcac cgtgatattg
    36901 ttcatgccgg gaatggcttc aaccacattc ggcatatcca ccagacgctg cgccagtcgc
    36961 cagatccgtt tctggctagc cagcgtcacc ggcggttcca gttccagcac taccgccgtt
    37021 tcacctatca gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc
    37081 aggattagga atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct
    37141 caatgcgcga ataccaccgc gttcagtggc atggtgacct gcagcataaa aatgcaatcc
    37201 ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac
    37261 gccaaaacgc gcggcgctat cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg
    37321 ctggaccacc tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc
    37381 aatccaggag gctagttcca ttcccggcac aggcatggtc agctctcccc acggcaccaa
    37441 cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc
    37501 cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac aggttgatat cattcgccag
    37561 caacgttttt aaacggttac gcttcattcc gcgaatgacc ggagactctc ctttccagaa
    37621 gtagccatga tgcacaatga ccgcatccgc gcccagacgc actgcctcat cgagcaaagc
    37681 ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ccacctgcaa
    37741 accattcggc gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc
    37801 cagttcggtg tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag
    37861 cgttacatcg cctgttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg
    37921 ccaaaagacg caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag
    37981 acagcatatt gcataaataa tcagatctgt atcaaagttt taactttaag gaaacacaag
    38041 cacgccgcgc aactattacg tgacgttgcc gtcaattgtg gaattattat gaataaacac
    38101 gcatcacaac cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc
    38161 ttttacggca tgcgtgccct gctgattctc tatctcacca atcaactaaa atacaacgat
    38221 actcacgcct acgagttatt tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc
    38281 ggtggctttt tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgttg
    38341 ttgatggcga tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat
    38401 ctgtccctgg cgattatcgt ctgcggctat ggcctgttta aatctaacgt aagctgtctg
    38461 ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat
    38521 gcggcgggta acgtggggtc tattatcgca cctatcgcct gtggttacgc ccaggaagag
    38581 tacagctggg cgatgggctt tggcctggcg gcggttggaa tgatcgcggg tctggtcatt
    38641 ttcttatgtg gcaatcgtca tttcactcat acccgcggcg ttaacaaaaa agtactgcgt
    38701 gcgacaaact ttctcctgcc gaactgggga tggctgctgg ttctgctggt ggcaacgcct
    38761 gcactgatta ccgtgctgtt ctggaaagag tggtcggtat acgccttaat tgtcgcgact
    38821 attattggcc tgggtgtact ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgg
    38881 aaggagctgg ggctgattgt gacgctcacc ttcttcagta tgttgttctg ggccttcgca
    38941 caacagggcg gtagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatgttt
    39001 ggttataccg ttccgaccgc gatgttccag tcgattaatg ccttcgcagt tatgctgtgc
    39061 ggtgtgttcc tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc
    39121 tggggaaaat ttgctcttgg ccttggcctg atgagcgccg gattctgcat tctgacctta
    39181 agcgcccgct ggtccgcaat gtatggtcac tcttctctgc cactgatggt gttaggcctc
    39241 gcggtgatgg gctttgcgga actgtttatc gacccggttg ccatgtcgca aattacgcgc
    39301 attgaaattc cgggcgtgac cggcgtatta accggcatct acatgctgct ttctggcgcg
    39361 atcgcgaact atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct
    39421 tccggggcga tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg
    39481 ggcgcactgg cgtgtgtagg actggtactg atgatttggc tgtatcaggc gctgaaattc
    39541 agaaaccgcg cgctggcgct ggagtcataa taggccagca ccgatgcctg atgcgacgct
    39601 tgacgcgtct tatcaggcct acgtatttcc tgcaatttat tgaatttgca caaatttgta
    39661 ggctggatta ggcgttcacg ccgcatccgg catctgggct cgattgcctg acgcgtctgt
    39721 tatttcccct tccgcgccgc ctcatacgct gccaacgttt gtactctcgc ctctttgtgc
    39781 tcgactatcg gttgcggata atccagcttc acacctgctt tctgcgccca cttccacggc
    39841 tcatgcaccg ctttccctgg tacatcgcgc agctccggta accatcgacg aataaactcg
    39901 ccctcacggt caaatttctc gccctgagtt atcggattga aaatacgaaa atacggcgca
    39961 gcatcagtac ccgtagaagc cgcccactgc cagccaccgt tattagctgc caaatcacca
    40021 tcaatcagct gcgacatgaa atatcgctcg ccttcgcgcc agtcgatcaa caaatctttc
    40081 accagaaaac tggctgtaat catccgtagc cggttatgca tccagccagt gctgttaagc
    40141 tgacgcatag cggcatcaat aatcgggtat cccgttttgc ctttctgcca ggcctgtaaa
    40201 tgtgcgggat tgctttgcca ctgcacacga tccgtccagg caataaatgg acgatgttta
    40261 cacaacgagg ggtaatacgt catcagatga cggtagaact cgcgccagat cagctcacta
    40321 agccagacac taccggcccc accgtccagc gcctgcggct gttccgccaa caagcgatgc
    40381 aaacactggc gaggcgataa cccgcccgtt gccaagctgg cggacaaacg gctggtgcct
    40441 tccactgccg gaaaatctcg ttgttgctca tactctccag caccgttctc gcaaaactgg
    40501 cgtaattgcg caatcgccgc tttttcttcc accgggaaat gcgcagtatc gaaagactga
    40561 cgaggataat tcagcgtaat ggatggcgcg ggctttatcg atccgctact acgaactttt
    40621 ggtgcagcga cgcactccgg catcccttcc cgcagccgtt tcagccaggc attcttaaaa
    40681 ggcgtaaaga ctttgtacat ctcgtgattg ccggtcatca ccgcccccgg cggcaggatc
    40741 acgctgtcat caaatccttc acacaccacg ttacgcagcg ctctttcaac gtccacatcc
    40801 cgcgctcgct cattcacctc atactgatag ttataaaaaa gatgagtaac gctgttttcc
    40861 gcgcacacct gtttaactat ttcgacactg gcgacaaagt catccacttc acggaacaat
    40921 aaaggaatac ctttttccgc cagcgcgatt tgtagcccat tcagttgagc attgataagt
    40981 tcagcctgac gcggcgacat gttatgcgtc tcccacttgc gtggtgtagc gatatacagc
    41041 gccagcacgc gtgcagacga attgcggcag gcggcagcca gtgcgagatt atcgtgcaga
    41101 cgtaaatcct ggcgaaacca ggccagatgg gtagtcataa aactcctgaa tggcgcaagt
    41161 taatcacacg cggccaaagt tcgaaataac gctgccctga aagccaggcg tcaggataaa
    41221 gcgccatata gtgtttgata cgcatcagcg gcgcaagaag tggttgctcg ccacggcgat
    41281 aactgtcgat aagcgcagcc agctgctggc gttctgtgga atcaatatgc gggcgaaaat
    41341 aaccctgaac gtgcatcaaa acgttggtgt gatcgcgcgg gttggcggga tgagaaagca
    41401 aaacaattac gcgttggcgg tactggttgt aaaaatcatc cagattttgc cactgatgaa
    41461 tatcagccac aaacgggcca agtttgcggt actcgggctg agaatgggcc agaaagacga
    41521 gtttatagcg gctgtgataa tcgagaagcg ccccgcgcgt taagccatgt tctttcagtc
    41581 gatgtaactc atgaagggcg aagatacgga ttagggtgct gtcatcaaat cgttgtagat
    41641 tcattactgc tcctgaacgg tatacaggag taagtgtagg cgttggtaaa tgatgtttgt
    41701 gaagttaatc cgctagcagg gcaagattac gaaagcgcgc tccccgcaag caactaatca
    41761 aggtagttaa tcatggctga acacgatatt acgccagaag tcactatttc caaaacacag
    41821 cgccgctata aagtcggcta tgtcagagcc cgccaccagg accgcaaaac cggtatagcc
    41881 cggtattatt cacaacatcc cagtctgcac cttaagggcg actggctgaa agaggccgga
    41941 tttgataccg gacggggtgt cacggtgaag atttcggagg ggggcctgac catcatcgct
    42001 gacagtgacg aggtgcagga actgcgggat gggatgttta gtgtgctgaa cgaaagttaa
    42061 atattaaaaa agctgggaag atttaaaacc ttcctagctt tttattctgt cttattataa
    42121 tggatactca tcatcttccg atgaatttat aaattgctcc aagtttacat gaagagtact
    42181 taacctagtt tttaactcat taagtgaagc gataatttca tattttccaa cataatcata
    42241 tgatggatcg tattcattat ctatgtcaga agctaaaaat gatgatattc gtagtttaaa
    42301 tttagcttca tctttattac attcattggc ataaggtgaa tgccctatca tagcatattc
    42361 atctaatcta aaaggataag atggttttaa ttcatcgatc aatgaaattt caatccatgt
    42421 tctatatcca ggcattaatg gactaatttc tttgagcaaa acctttccac ttaataatgt
    42481 attgagattg ttcattatta tgtctcccac gggaaaaata tatgtctaac acttccacca
    42541 tgttgatgaa cttgtaaatg tggtaaatca ccatcaggat ttcctggtcc atgacctgac
    42601 actctatatg aaggttttgt tacagaagga tcttttatat catcccaatg atatgaacct
    42661 gcttttgttt tattttcagt aagatattcc attaagttct tagtgctggg gcctgactca
    42721 cctgtcatat ttttataatt atgacctaaa tatctatcca taaacaattg ttcagcgtca
    42781 cttctagacc taacagtaac tttggtttca ctactatcta ataaacgatt cgctttttgg
    42841 ttactaacag cacatggagt taatccaagt ggatcagcat attttagagg attaggtgca
    42901 taactgtaga gatttaaacc gcctctcaac cctatcgggt cagttaccat gaaccgcccg
    42961 acatccggat catagtacct gtgcagattg tagtgcagcc ccgtttccct gtcgaggtac
    43021 tgtccctggt agcgcaggtt ctgctccacc ccgccgtggt ccttctcctg tatcgtgtta
    43081 ccccagacct gataccccgt ctcccacacg attttcccgt cgctgtccgt catctcttcc
    43141 ggcgcgccgt tcacatccgt gtgaaaatac agtatccggt tcgggccctc tttgcccgcc
    43201 ttatccaccc gcgccagcgg ttcataactc ccctggtccc tgtagatgta caggctgcct
    43261 gttccctccg gcctgctctc ctgcgccagc cgcattccgt cccataaaaa ttttgtccgg
    43321 ttgtacggct taccgtcagg gctgatgtgc tgtttttccg tccgcctccc cagtgcatcg
    43381 tacacatacc ggtatgtccc ccgcgctgtc gtcacttcca ccatccggtg ttccgcatca
    43441 taacggtaac tctgcgttcc gctccggccc tccttttcca ccgcacggcc aaattcgtca
    43501 taacggtagt gcacaccacg gtaagacatc agccggttga acggcaccac gctgttttct
    43561 gccgtggccc tttcccccgc gcgcctcccc agcaggtttc ccgccgcgtc cacctccgtt
    43621 tccggctggt gccacaccgg gtgccactgc gcctgctcca cccgccccag cgggtcgtgc
    43681 gtctccgtga tatgcccctg caggtcatac acatagcgcc atttgccgcc ctgagggtcg
    43741 gtcatcccca gcaacagtcg ttcttcgtcg ctccagcgga atgtcgtcac ctgtccggct
    43801 tcgtcctggt acgccgtcac ctgattctgc gcatcccact ggtgataacg tgtgcgcccc
    43861 agtccgtccg tcacgcacag cctgcgctgc gccaggtccc acgccagccg gtactgttca
    43921 ccgctgtccg tgtggtgccc cgtcacccgc catgccgcgt catccggacc ttcataccag
    43981 ccccactggt aataataccc ttcgccgccc ggctggcggt gcagcgtcat gcagtggttt
    44041 tcgtcgtaag caaactcacg cagcaggtgc cccgtgctgt cgtacacgcc gttcaggtgg
    44101 ccggcctcgt catagctgta acgacgcagc agtaaaggtg cagtctcagg gaaatgctga
    44161 taaatctgtg tcacccgccg gggatgcgcc gccagttcgt aatacagacg gatgcacgga
    44221 cgctgctgtt cgccgctgat ttccacaatg cggccccggt cgtcgtagta cagattcaga
    44281 cagttgctgt tacggtcgcc cagcattttc agccgctggc gctgcgggtg gtgcgggtca
    44341 gcctcaaaca gaaaacactg cccgtcatcg ctgctgataa gccagtgccc ctgctcacca
    44401 cgccttaccg ccagcccttc accggggctg taaaacccgc cccccggcag tacggcttcc
    44461 atatccaagc gtcgccccat gcccgacaca aaggtcatgc agttttcgtc tgcgctaccc
    44521 ggtgtgcaca cgaggctgac ttcatacatt acactccagc cctgaccaaa cattccttcc
    44581 gtacgttcgt cacgactgct gtacacgcgc tgccagtcca gcggaaagtg gcccggcaga
    44641 ctgaaatcga agtcatcgtc atcggccagg tatttggccc cggtggcggc attgaccgga
    44701 tgcggaaggg cactggtggc cttctgcgtc agcatgctca tcccgacact ggcagcaaaa
    44761 caggcgattt tagaaagcat tttgcccggg cgaagaaaca tcagcgccac ggttgccacc
    44821 agggttatct gcgacctgcc gcttctgata tccctgacca ccagtaacgg accgccaatg
    44881 cgcacatccg gtgacacaaa cgccccgttc cccggctcat tcaccaccct ggcctcgcag
    44941 gtacagcgtg ccccgctgcg tgtcgccggc tggctgttga tacgcacatc ccgcgcgcct
    45001 tccgcgatat attccgctgc gggcggctcg tcatcgcctt tgaacagatt actgatgctg
    45061 ccaataaccg cattcactcc ctgaaaggcg gtttccagca taccgcccgg attcagcagg
    45121 ttaagggccg ttcccagggg gtcatccaga aaggcggctt ttttttgtgc gagaaactgc
    45181 gtcagtgagg aaggatgttt atggcggtcg attttatcgt caggggtcgg ggtggatccc
    45241 ggggccgcac tggccacagt gggctgccat agttccgaca caaaatgtgt cacgccatcc
    45301 accatttcag aacgcccttc ttccgtactg agctggtcgt gcagagcccg ggacatcagc
    45361 acaggccctg tcaccgggtt aaccgccagg gctatggccc ccgccatttc cagatattcc
    45421 ccggccacat cccggaacgt tctggcatcc tcctgacgct gcgcttcttc ctgttctgcc
    45481 ttcgcctgcg cttcctgcgc ctctatgtcc tgccgcgatg ccgcagtcgc cgctgcccgc
    45541 gcggcaggtt tgctgttgat ccatgtgtca ccagagccgg tgaggatgta accttcaatc
    45601 tgaggcggga aaagcatatt tgcgagggct gaacaggctt cgctgacctt ttctgtcacg
    45661 ccggtgtaca ccatcgccac acccgccaca aaaccggtca gcacgacggc cgtgcacccc
    45721 atcgtgatcc ccactgacgt cgccagtaac gaggttgtgc cgacaatgag ggcatcaata
    45781 acgacatttg cggccacctc cagcaccgcc cccagcacat ctgccataaa cgacgtatgc
    45841 agcagggcat caccctgtct tgccgcccag aatgcctctt ccatgtcccg ttccctgtat
    45901 gattaattcg tcctgacttc ctgccgcatc ctgaagcgca gattcttttt aatttcagcc
    45961 cagcgcgtca tatctgccgg ggtcagcggt gtggttctgg cgtaggtgaa ggccatcatg
    46021 acccgctcac ccggcaatac aaatgccacc tgacactggt acacctgctc gttaccccgg
    46081 agaaactggc tctgtatttc caaaccgcct cgttgagata atattccgtc ataatgtaaa
    46141 tagtccgttt taataaagga attatttatt gacaggttct gtttttttaa tcatgaatgt
    46201 aatacccatc accgctttac agcacacttt atctttccag tcataatcac taataatatt
    46261 aagcaattag caatatttac aacgaccaga gcggatacat agttatgtat ttaacaatcc
    46321 caatatattc actatgcaaa taaaaccact agtaacagac acgctaccgc ctccatataa
    46381 aaatggacac cttataaaat tgtatcttgc atagaaaaag ctgaaatatc ggatgcgttt
    46441 taattatcaa ctataaatct ataagctttt t
//
LOCUS       DAJVPT010000019        46266 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVPT010000019 DAJVPT010000000
VERSION     DAJVPT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(37..489)
                     /locus_tag="OQM47_001045"
     CDS             complement(37..489)
                     /locus_tag="OQM47_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1479289.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(542..1876)
                     /gene="adeQ"
                     /locus_tag="OQM47_001046"
                     /gene_synonym="yicO"
     CDS             complement(542..1876)
                     /gene="adeQ"
                     /locus_tag="OQM47_001046"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1479290.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            2051..3817
                     /gene="adeD"
                     /locus_tag="OQM47_001047"
     CDS             2051..3817
                     /gene="adeD"
                     /locus_tag="OQM47_001047"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1479291.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(3863..5254)
                     /gene="uhpT"
                     /locus_tag="OQM47_001048"
     CDS             complement(3863..5254)
                     /gene="uhpT"
                     /locus_tag="OQM47_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1479292.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(5392..6711)
                     /gene="uhpC"
                     /locus_tag="OQM47_001049"
     CDS             complement(5392..6711)
                     /gene="uhpC"
                     /locus_tag="OQM47_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1479293.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(6721..8223)
                     /gene="uhpB"
                     /locus_tag="OQM47_001050"
     CDS             complement(6721..8223)
                     /gene="uhpB"
                     /locus_tag="OQM47_001050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1479294.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(8223..8813)
                     /gene="uhpA"
                     /locus_tag="OQM47_001051"
     CDS             complement(8223..8813)
                     /gene="uhpA"
                     /locus_tag="OQM47_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1479295.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(8888..9178)
                     /gene="ilvN"
                     /locus_tag="OQM47_001052"
     CDS             complement(8888..9178)
                     /gene="ilvN"
                     /locus_tag="OQM47_001052"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1479296.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(9182..10870)
                     /gene="ilvB"
                     /locus_tag="OQM47_001053"
     CDS             complement(9182..10870)
                     /gene="ilvB"
                     /locus_tag="OQM47_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1479297.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(10976..11074)
                     /gene="ivbL"
                     /locus_tag="OQM47_001054"
     CDS             complement(10976..11074)
                     /gene="ivbL"
                     /locus_tag="OQM47_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1479298.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            11639..11728
                     /gene="tisB"
                     /locus_tag="OQM47_001055"
     CDS             11639..11728
                     /gene="tisB"
                     /locus_tag="OQM47_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1479299.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            12008..13192
                     /gene="emrD"
                     /locus_tag="OQM47_001056"
     CDS             12008..13192
                     /gene="emrD"
                     /locus_tag="OQM47_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1479300.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(13200..13697)
                     /locus_tag="OQM47_001057"
     CDS             complement(13200..13697)
                     /locus_tag="OQM47_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1479301.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(13694..14056)
                     /locus_tag="OQM47_001058"
     CDS             complement(13694..14056)
                     /locus_tag="OQM47_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1479302.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(14046..14393)
                     /locus_tag="OQM47_001059"
     CDS             complement(14046..14393)
                     /locus_tag="OQM47_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1479303.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            14502..14915
                     /locus_tag="OQM47_001060"
     CDS             14502..14915
                     /locus_tag="OQM47_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479304.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(14962..16404)
                     /locus_tag="OQM47_001061"
                     /pseudo
     CDS             complement(14962..16404)
                     /locus_tag="OQM47_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(16439..18154)
                     /locus_tag="OQM47_001062"
     CDS             complement(16439..18154)
                     /locus_tag="OQM47_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCS1479305.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVIITFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            18321..19214
                     /locus_tag="OQM47_001063"
     CDS             18321..19214
                     /locus_tag="OQM47_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1479306.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(19211..20025)
                     /locus_tag="OQM47_001064"
                     /pseudo
     CDS             complement(19211..20025)
                     /locus_tag="OQM47_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20025..21641)
                     /locus_tag="OQM47_001065"
     CDS             complement(20025..21641)
                     /locus_tag="OQM47_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1479307.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            21936..22610
                     /locus_tag="OQM47_001066"
     CDS             21936..22610
                     /locus_tag="OQM47_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1479308.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(22649..24310)
                     /locus_tag="OQM47_001067"
     CDS             complement(22649..24310)
                     /locus_tag="OQM47_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1479309.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(24506..24934)
                     /gene="ibpB"
                     /locus_tag="OQM47_001068"
     CDS             complement(24506..24934)
                     /gene="ibpB"
                     /locus_tag="OQM47_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1479310.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(25046..25459)
                     /gene="ibpA"
                     /locus_tag="OQM47_001069"
     CDS             complement(25046..25459)
                     /gene="ibpA"
                     /locus_tag="OQM47_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1479311.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(25453..25525)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            25764..26096
                     /locus_tag="OQM47_001070"
     CDS             25764..26096
                     /locus_tag="OQM47_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1479312.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(26098..27360)
                     /locus_tag="OQM47_001071"
     CDS             complement(26098..27360)
                     /locus_tag="OQM47_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1479313.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTNTNTNTWTPDSQWLVF
                     DVRPSGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPD
                     ETWHYDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYN
                     DHVMHQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEI
                     NRAYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTL
                     PAPPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISP
                     QGGEPRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPS
                     ADAVVFSPDGQWLAWMEGGQLWITETDR"
     gene            27461..28561
                     /gene="cbrA"
                     /locus_tag="OQM47_001072"
     CDS             27461..28561
                     /gene="cbrA"
                     /locus_tag="OQM47_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1479314.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(28522..29859)
                     /locus_tag="OQM47_001073"
                     /pseudo
     CDS             complement(28522..29859)
                     /locus_tag="OQM47_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(29934..31082)
                     /gene="dgoD"
                     /locus_tag="OQM47_001074"
                     /pseudo
     CDS             complement(29934..31082)
                     /gene="dgoD"
                     /locus_tag="OQM47_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(31079..31696)
                     /gene="dgoA"
                     /locus_tag="OQM47_001075"
     CDS             complement(31079..31696)
                     /gene="dgoA"
                     /locus_tag="OQM47_001075"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1479315.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(31680..32558)
                     /gene="dgoK"
                     /locus_tag="OQM47_001076"
     CDS             complement(31680..32558)
                     /gene="dgoK"
                     /locus_tag="OQM47_001076"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1479316.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(32555..33244)
                     /gene="dgoR"
                     /locus_tag="OQM47_001077"
     CDS             complement(32555..33244)
                     /gene="dgoR"
                     /locus_tag="OQM47_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1479317.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            33522..34178
                     /locus_tag="OQM47_001078"
     CDS             33522..34178
                     /locus_tag="OQM47_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479318.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(34224..35036)
                     /gene="yidA"
                     /locus_tag="OQM47_001079"
     CDS             complement(34224..35036)
                     /gene="yidA"
                     /locus_tag="OQM47_001079"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1479319.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(35150..35548)
                     /locus_tag="OQM47_001080"
     CDS             complement(35150..35548)
                     /locus_tag="OQM47_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1479320.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(35788..38202)
                     /gene="gyrB"
                     /locus_tag="OQM47_001081"
     CDS             complement(35788..38202)
                     /gene="gyrB"
                     /locus_tag="OQM47_001081"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1479321.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(38231..39304)
                     /gene="recF"
                     /locus_tag="OQM47_001082"
     CDS             complement(38231..39304)
                     /gene="recF"
                     /locus_tag="OQM47_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1479322.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(39304..40404)
                     /gene="dnaN"
                     /locus_tag="OQM47_001083"
     CDS             complement(39304..40404)
                     /gene="dnaN"
                     /locus_tag="OQM47_001083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1479323.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(40409..41812)
                     /gene="dnaA"
                     /locus_tag="OQM47_001084"
     CDS             complement(40409..41812)
                     /gene="dnaA"
                     /locus_tag="OQM47_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1479324.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            42109..42189
                     /locus_tag="OQM47_001085"
     CDS             42109..42189
                     /locus_tag="OQM47_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479325.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            42419..42559
                     /gene="rpmH"
                     /locus_tag="OQM47_001086"
     CDS             42419..42559
                     /gene="rpmH"
                     /locus_tag="OQM47_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1479326.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            42576..42935
                     /gene="rnpA"
                     /locus_tag="OQM47_001087"
     CDS             42576..42935
                     /gene="rnpA"
                     /locus_tag="OQM47_001087"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1479327.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            42899..43156
                     /gene="yidD"
                     /locus_tag="OQM47_001088"
     CDS             42899..43156
                     /gene="yidD"
                     /locus_tag="OQM47_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1479328.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            43159..44805
                     /gene="yidC"
                     /locus_tag="OQM47_001089"
     CDS             43159..44805
                     /gene="yidC"
                     /locus_tag="OQM47_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1479329.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            44911..>46266
                     /gene="mnmE"
                     /locus_tag="OQM47_001090"
     CDS             44911..>46266
                     /gene="mnmE"
                     /locus_tag="OQM47_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1479330.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CI"
BASE COUNT        11342 a        12526 c        11761 g        10637 t
ORIGIN      
        1 caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt gaacgccctc
       61 ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct gcatttcagg
      121 ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa tcggcgcgga
      181 aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc tatcatcgac
      241 aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg actcatccgg
      301 gcgatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat cgaccatcga
      361 ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg cttttctggc
      421 cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg cgagcattat
      481 ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc ctcgccagtt
      541 actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc acacacaggt
      601 tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata aagccgagtg
      661 caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata aacgcaggca
      721 ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc agcacgccaa
      781 caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc ggtgagaaga
      841 acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg cgaccaccga
      901 ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg ataaatgcgc
      961 ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg aatttaccgt
     1021 tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat gaatcaaata
     1081 ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac gcgccgctca
     1141 aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg ccgctaaaat
     1201 gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc agcaccgcgg
     1261 catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata cctaacaaca
     1321 cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg acaataacac
     1381 cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg ataccaatac
     1441 gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc gtgagcaaaa
     1501 atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag atccccatcg
     1561 cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc agcgccacgg
     1621 gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa caggtggtaa
     1681 caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc acaaaaacaa
     1741 tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg acggtggtcc
     1801 catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc acgtaatcgg
     1861 tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc ttttgtaaaa
     1921 caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt aatagatatt
     1981 ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat aaaatcaaaa
     2041 tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc gggctgaata
     2101 ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta ttgataatgt
     2161 ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga ttaaaggacg
     2221 ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc ggattgatgc
     2281 tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg aatccagcat
     2341 gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga ccgttatttg
     2401 cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt ttgcccgctg
     2461 tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc ccgctctgga
     2521 aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct ggcgggacca
     2581 tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta acgggcagaa
     2641 tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc actgtccggg
     2701 tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact gccacgaaag
     2761 ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga tgatccgcga
     2821 agggtccgct gccagcaatc tcaacgcact ggcaccgttg atcaacgaat ttaacagccc
     2881 gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg aagggcatat
     2941 cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg tggcatatcg
     3001 cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct tactggcacc
     3061 cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg tgcagcaggt
     3121 actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt cggcgagact
     3181 ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt ccgccagcga
     3241 ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca tccataacga
     3301 attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc gcgatgatgt
     3361 gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct gtggtttgct
     3421 cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg acagccataa
     3481 tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc aggtgattca
     3541 ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc tgccgttacc
     3601 cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa ttgacgcctt
     3661 gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc agatggcgtt
     3721 tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat ttgatggcga
     3781 gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg gcgtcatgcc
     3841 gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt ttctcgcggc
     3901 ggattttgcg ttcttccatt accgccacta tcgccatcag gcagatacaa ccaatcgcgg
     3961 cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc ggcgtcccat
     4021 cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa gcaaaggtgc
     4081 ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca gcagccacac
     4141 caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa gccagataga
     4201 tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc gccagcgcga
     4261 tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc cacagcagcg
     4321 taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc gctttagaga
     4381 gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata cgtaccacat
     4441 agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc acatactcaa
     4501 caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct tcgctgatct
     4561 cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag tcgctgccgt
     4621 aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata aacatgccga
     4681 tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct gcgcctgcac
     4741 cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta cgacgcggcg
     4801 tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa aagccgctta
     4861 aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata ctggcactga
     4921 agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt ttggtgtttt
     4981 tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg gagaaaccca
     5041 ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata tcgttctgcg
     5101 cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag accaccaggt
     5161 aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga aggtccaggg
     5221 tcggcttgcg aacttggttt aggaaagcca gcatgggtta ctcctgaaat gaatacctgc
     5281 gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt ccaagaaaaa
     5341 cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc atcacgcttc
     5401 acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc cggcggcgat
     5461 agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg ccagcggcca
     5521 accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg ccgcccctgc
     5581 cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg ggccaaagac
     5641 aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa atggcatcag
     5701 ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca tcggcccacg
     5761 gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga taaatccgcc
     5821 cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca gcgtctcgga
     5881 catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca gcacatagca
     5941 aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga tctctttacg
     6001 cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc gccattcacc
     6061 gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga gaaaaatccc
     6121 cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt aatgcagcgc
     6181 gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg tgttccataa
     6241 tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac gcgcacacac
     6301 cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa acgcccatag
     6361 cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc ctatccccat
     6421 aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg acacgccgta
     6481 agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca cgccgttagc
     6541 aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca gcgcgtaacc
     6601 cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat caatttcatg
     6661 tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca tcatcgaacc
     6721 ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac aggaaatgtg
     6781 taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa aaccttgttg
     6841 cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca accgttcatc
     6901 ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa tgttgttcag
     6961 cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg ctgattcatc
     7021 gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt cccgcatcag
     7081 tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta accgtcctaa
     7141 caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct gcccgctctg
     7201 cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag tacgaatagc
     7261 agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc gcacgctctc
     7321 ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct tttgtagcga
     7381 ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg tcagactttg
     7441 caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac tggcgatcag
     7501 cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt gccaggccag
     7561 cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt ccggcaatcc
     7621 caactggagc cagagactga taacaaacag cagcaagtac cagaccagat gccgcccgcg
     7681 ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt tattggcgag
     7741 atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta aagtcagcaa
     7801 cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca acaacgccgc
     7861 cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat ggcgatagcg
     7921 cgagatcagc gctacgggca gtaacgtcag caaactaccg atcatcaata acggaaaatg
     7981 ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac ccagcaatac
     8041 gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa acggaaataa
     8101 cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata ggcaaaacca
     8161 tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga acaacgtctt
     8221 catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac gcccagtttt
     8281 tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag ttcggcggca
     8341 atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc acgtttggtc
     8401 agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag ataacagccg
     8461 cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg tttggaaaga
     8521 aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc gtgaacggag
     8581 agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc ggagatatcg
     8641 ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc cgccagcgcc
     8701 tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc cagcagctgc
     8761 gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat catggtcttg
     8821 tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct
     8881 tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg gactgattac
     8941 gctgcacttt cacgacatct tccagcttag cgatttggct tatcatctgc tccagacgct
     9001 ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc ggcagacaaa
     9061 gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg gtcattacgc
     9121 ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg ttttgcatgg
     9181 cttattcccc caccatttca gtattcgccg cacctggcgg caccatcggg taaacttttt
     9241 cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatg atttcctgca
     9301 atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg aatccggcgg
     9361 caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct tgctcgtaga
     9421 acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga atgattttga
     9481 catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc aggctgccgt
     9541 cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca atcgccgcag
     9601 gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt gggcgattga
     9661 gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg ataatcgcat
     9721 tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat ggatcgcacg
     9781 ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg tgccactctg
     9841 cacgcggttg cgcttccacc cgcgggatca attgcgccag cacgtcatca acatccgcct
     9901 gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata tcgacatgaa
     9961 taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg
    10021 caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg cgcacgccgt
    10081 gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc agcgccatta
    10141 aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc gcaggcgcat
    10201 tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc attgtagctg
    10261 cgtcacgaat gctttcttcg ctaaaggcgg gtgcaacggc tttttctgcc accgcgggct
    10321 gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc cacaccgggc
    10381 tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg agctcttcga
    10441 tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag gtgtccactt
    10501 cttggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg caaatcagcg
    10561 ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca cccggcccgc
    10621 tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga gcgataaacc
    10681 ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg cttaaggcgt
    10741 cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt
    10801 ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg
    10861 aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa
    10921 aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc agtccctacg
    10981 gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat ggcgcagttg
    11041 gtagtagttt tgcgttgaac atggaagtag tcattgggga ccttgttggt tttgtgttta
    11101 acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt cagcaaggga
    11161 aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac cccgccggag
    11221 cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt atgtcaacac
    11281 tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg acgtatttag
    11341 tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt ttatttatac
    11401 agtaagcttc tataatatca ctgtacgcaa tgtgttatgc gggggccgca tcgttacccg
    11461 gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc gcgtgagcac
    11521 actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca caacagctgg
    11581 caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg agacgcgtat
    11641 gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac tgcaactgct
    11701 tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa ggggagcggg
    11761 taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg ctgacgcatc
    11821 ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta catattctgc
    11881 cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat gtgatagatt
    11941 cctctcccgc atttatggga atgcgtagtg acttattcta attattttta taaaagcatc
    12001 cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat tactcgtggc
    12061 cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg cgcgcgatct
    12121 caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga cttacggtgt
    12181 ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag tgatcctcgt
    12241 cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca gtttgacggt
    12301 attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg taatggcgcg
    12361 tactttgccg cgtgatttat atgaacggac acagttgcgc catgctaaca gcctgttaaa
    12421 catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc tggatacgat
    12481 gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg tgaccttcag
    12541 tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc gcctgcttac
    12601 cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga tgctgattgg
    12661 cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg gcgcggtgtt
    12721 agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg cggcattttt
    12781 tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt ggcagtcggt
    12841 tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg gcgtgatgaa
    12901 tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga tgctgtttcc
    12961 gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg ctggtgcgct
    13021 ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg cgatgttgcc
    13081 gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt tgatcgtgct
    13141 gtgctggctg ccgctggcga cgcggatgtc gcatcagggg cagcccgttt aagcgcacgt
    13201 caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag atcacgcatc
    13261 acccgcatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca aagcacgctg
    13321 tctggcgtct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc ctggcagtca
    13381 cggcgcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc aaaaagctca
    13441 atcgagatac gactgatatc tgcccgcacc caacactcaa acagggcaat taaaaatttc
    13501 ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc aaattcaaga
    13561 tagggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata aaaatcggca
    13621 ggttgcagag catccttgta ctctacaacc agcttatgcc tgtcctcttc agcatcaatg
    13681 acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt atatgcgtta
    13741 cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg tcacggacta
    13801 cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta cgggtgtagt
    13861 gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat aacatgcccg
    13921 cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc agcatggtac
    13981 gaaaccacgc cagtgatgtg cgctccggct gcagccctgg gtcggcaatg cgtctggctt
    14041 tacggctatc cggcatacaa caccagtccc ataacgatca ccgccacaac catcaatatt
    14101 aagctgatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg catcgctttt
    14161 tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa cagacacaac
    14221 aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag ttgatcaagc
    14281 ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa agtacgctca
    14341 ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt cattgttgct
    14401 cctttgcaat ttcactaacg ctctatcttt agcggttttt tttaagaatt tcgtccgccc
    14461 gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc atagcgcaaa
    14521 ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg gggattatcc
    14581 acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc gtggcggaca
    14641 aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa attaccactg
    14701 tggagaaaaa agaggacatg gatatcgata aaatcctcga tcaaagcggt atcgcactgg
    14761 ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat cgctggggca
    14821 ttcgcggcgc gctggttttt ggtatcggca tcgtgtgcag cattttgctg atattcctga
    14881 ttttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg cccgccgggt
    14941 gatcatcagc gggcattatc gtcagaattt ctgttttttc tcctccacct tcactccttg
    15001 cgtcggtagg ccggtgtcat agtcgcgtac cactggcgaa tagccatctt gtgggcgtgg
    15061 gcgaaacgcc cccatccagc gcggtcgtgc atctttacgc cacggacgca gactccattg
    15121 gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt catgcatttt
    15181 gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat ttgggtcatt
    15241 acggcgatcg taaagttcat cactggtgaa gaggttgagc accagtttaa agtcatccgt
    15301 cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct cgtagcggtt
    15361 aaattccacc atcacgccgc gtggctcttt cacggcaagg atattttccc ccggcagaat
    15421 ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcaa tatgactgac
    15481 tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg gaatacgggt
    15541 gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca tcatttcacc
    15601 gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta aggcattgat
    15661 gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg gatggtgata
    15721 aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt gatgttccgg
    15781 tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat cagcgtattt
    15841 ctctaaatac tccaccggac aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat
    15901 caggaagggt tcctcggcgc gcacgggcta ttgcagaaaa tctaccgctc gattgctgat
    15961 gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt cgacgctgtt
    16021 taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat agttcgcccc
    16081 atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga aatagtcatg
    16141 accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct taaagtagcg
    16201 ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc cggactggtt
    16261 agcgtagata ccggtgaata gtccggcccg tgcaggcgta caaaccggtg aacaagtgta
    16321 ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg tattcagcgg
    16381 tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt ggggcgtttc
    16441 ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga tgccaccagc
    16501 tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc atcgctaacc
    16561 agcgcgtacc gtagccgccg aattcagcca gcccgacata gacgccaatc atcgcgaaca
    16621 ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc gcaaacgcat
    16681 ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc atcacgacca
    16741 cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac ttcaccagat
    16801 agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc gccagcgccg
    16861 ggatgcgcgg aaagaaaaag cccataatga tgatggtaac cagcggcacg ttgtaaatgc
    16921 cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttcgcgatc cacggcgcta
    16981 ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg accagctgct
    17041 gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat aatgtactgg
    17101 cgctattcag aaagccgttg aaggtaatga tcaccgcacc aaataacacc gcgccgaaga
    17161 aaccctccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc atgtcggctt
    17221 tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt accagcgggt
    17281 cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc aggctttttg
    17341 acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg ttcaccagaa
    17401 ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg actgagttta
    17461 atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg cccttgccta
    17521 tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca ataccgttga
    17581 tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa attcccgcca
    17641 gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt agcgattccc
    17701 cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc ggcagaaagc
    17761 agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt ttatcataac
    17821 gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc ggtaaaaaga
    17881 ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc accgacatgc
    17941 cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg ttggttaaca
    18001 ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa tagccttgtt
    18061 gcgatccggt atctgttttg cgaaccttcc accaggtgat caccgccacc agcagcgtaa
    18121 aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt atgtttatgt
    18181 cccggacgca tgaatatagg gagaggtaaa atgcgtcggg tcaggcagtc agattcttaa
    18241 aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt gtgagcataa
    18301 ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta aaaaacgaaa
    18361 ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga atttctctga
    18421 tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg cgggacaaga
    18481 agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga tgtgggaaaa
    18541 ttcttttaaa gaatggcgaa caaataactc tgcatagcaa ctgtattata tttttaaagc
    18601 caatggatat tcactcttat cactgtgaag gtttagtctg ggaacagtac tggatggaat
    18661 ttacccccac cagtatgatg gatatttccg ttggtcagca aagcgttatt tataatggcg
    18721 aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca gaagcaataa
    18781 aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt tgtcttatgt
    18841 ccgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt gccactttac
    18901 atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc ccctgtagcg
    18961 aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa gaatattacc
    19021 tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac tcggttggcg
    19081 aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc tttaaacata
    19141 aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca acggatgcca
    19201 gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc aactcatcca
    19261 gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc gcctgccaca
    19321 ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc gccacttgct
    19381 gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt tcaggccgta
    19441 gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca tccggctttc
    19501 aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca gacgacgcgt
    19561 ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt ttggtgaata
    19621 tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc cgtacgctat
    19681 tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga ttttttcatc
    19741 cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat caacgtcgct
    19801 gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt tttctttaag
    19861 gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat cataaaattt
    19921 cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga tgcctggcgt
    19981 aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac tgcctccgta
    20041 atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg ttcacgcaat
    20101 tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa gacgccgtgc
    20161 gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg cagtgcaata
    20221 cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc cagggcttgc
    20281 aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt ttggcctcgc
    20341 gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg tcccggcgtg
    20401 cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag ggtaaagcac
    20461 aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat cgggatccag
    20521 ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac accaaagaga
    20581 tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag caacggtgaa
    20641 atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc ggttaaggtt
    20701 gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt gaagtacatc
    20761 gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc aaaaccgcct
    20821 tccaggaaca acgatttcag tggctcggcg ctcagactga actcttgcag atgctgcgcc
    20881 cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg tccgtagatg
    20941 aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac ccacacccca
    21001 agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg tacttttggc
    21061 cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat aatcaccaca
    21121 taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa cagacggtta
    21181 tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt atcgagggtt
    21241 ttaatcccgg ccatcattgt cagaccgcta cctgccaccg cgtcctgagt gaaatcgacg
    21301 ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca ggtcaggaaa
    21361 ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc aatgggtaaa
    21421 ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc ttcaataatg
    21481 tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac aaacatcggg
    21541 ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag cagcactggc
    21601 gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc ctcttttctc
    21661 aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag gataggaaga
    21721 tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg cttttcgttg
    21781 ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag gtacacagca
    21841 tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact tgtgggcgca
    21901 ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc attgcggagc
    21961 ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cagccttctt cccggtgaaa
    22021 aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc attgacctgc
    22081 tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg gtgcgcaaag
    22141 atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa cggcagggaa
    22201 aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg gccattgcca
    22261 gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg
    22321 aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc cgtaaccttt
    22381 cgctgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc gggattttga
    22441 ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg gcgcggcaaa
    22501 tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat agcgagagcg
    22561 gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag gtggagtacc
    22621 atttacggcg actccacccg gaaaaaagtt aaccgatact ccagaagagc accgccagta
    22681 attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg taagagagcg
    22741 ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga ggatcggtca
    22801 tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac atccgcgcca
    22861 gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca atccagctta
    22921 gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga ccaacgaccg
    22981 agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc ggcataaacc
    23041 agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc agcggaccgc
    23101 ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga atagaaccta
    23161 acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt tgcagttttt
    23221 gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc cccaccaggt
    23281 tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg accccggcac
    23341 ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt
    23401 ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg
    23461 tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt aaatccgctg
    23521 gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc gaaggtactt
    23581 ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg agaatcggta
    23641 catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg atagtcttga
    23701 tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc tcgacattga
    23761 cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg ccgaatggat
    23821 acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta cccaggtcgc
    23881 gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg gagaaaatcc
    23941 ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta accagaccac
    24001 cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg
    24061 caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc aggccaaatt
    24121 cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa acaaaatggc
    24181 cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg cgaaatttga
    24241 cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc gttaatgcta
    24301 tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat tatggctgaa
    24361 tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt gccgaaatat
    24421 cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag ccccaccaga
    24481 atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg ctgatagcga
    24541 tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc agtaaaccgt
    24601 ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc aggctaaatg
    24661 gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc tccggcgtgc
    24721 cttttacgct caggcgcgtg ccttccagtt gaatctctaa atcttcctga cggaaacctg
    24781 ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg tacggcggga
    24841 agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt
    24901 gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct aagaagcgag
    24961 taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat ggagatgtca
    25021 ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt tttttcgctt
    25081 ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta gccccacgaa
    25141 catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg atgccctggt
    25201 acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc agcagattat
    25261 cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata gcaatgcggt
    25321 aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta ctctggctct
    25381 ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg taaagcgggg
    25441 ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt aagaccttcc
    25501 acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg actacgatgt
    25561 aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat
    25621 ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacagt
    25681 gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc
    25741 cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca
    25801 gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg gggaaagaag
    25861 gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa accaactggg
    25921 gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtaatggac acgcttttac
    25981 tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa aaaagcgaag
    26041 ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat aactgattta
    26101 gcgatcagtt tcggtgatcc acagctggcc accttccatc cacgccagcc attgaccatc
    26161 cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc
    26221 aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa agcccaacca
    26281 ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactt
    26341 gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatcacg
    26401 cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc gcgggacgtt
    26461 gactaacccc ggataagccc gatgatgggt aaaggttaaa cgtcgctgca cgacgccacg
    26521 cggtggcgcg ggtagggttg tttccgttcc acttaacggc gcatcacctg ccgatttcca
    26581 gctagcttca tcttgcggta actcaacgat aaatagctcc ggcactttct cgccctttgg
    26641 cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc
    26701 acgattgatt tcatcactgc caggctgcgg cgtgggcgtg gttttactca ccagcacaca
    26761 ccagtggcta ccgctgtatt cacgcggatg ttgcttttgt acgttgaccg ggccaaacgg
    26821 tgcagcaacc ccgacgtttc gcaaatccag cgccggatcg agttgatgca ttacatggtc
    26881 gttataggta aagctccccc tttcaccgtt cgggctaaag acatgcacat ggctgccacc
    26941 gcgcagcgct cctggcgtat acggcgcggt aatatccatt gcatcgagat tgctcacctt
    27001 gccgccttca gcaatgactc cgcgtcgatg atggaagtcg taatgccatg tttcatcagg
    27061 attttcaggg ccgtgaataa agacatattt ctctgacttc ggatgaacgg tcaccacgcc
    27121 gacgtgtgcg ccctgtgacg cgcgatagat aacctcgacc tcgcctgtat ggatattcac
    27181 acgctcaatg gtctcgccgg taaacgacgc cccagaagga cgcacgtcaa ataccagcca
    27241 ctggctgtcg ggcgtccagg tattggtatt ggtattggta ttggtattgg tattggtatt
    27301 ggtattggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
    27361 ggcgcggtcc tgatacaaaa cggttcccgc catcatactt cacaaggact tcactttcag
    27421 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
    27481 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
    27541 ggtgatcgcg ctggataaaa agcaccagtg cggtactgaa ggtttcagca aaccttgcgg
    27601 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatggactga cgcttcctgt
    27661 cgatgtgatc gccaatccac agattttcag cgtcaaaact gtcgacgtcg ccgcatcgct
    27721 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
    27781 gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc gaaaaatctg
    27841 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc aacatattac
    27901 cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
    27961 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
    28021 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
    28081 caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
    28141 cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
    28201 tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg gtaaggacaa
    28261 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    28321 ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc cggagaagag
    28381 caacgccgct tactggcgcg ccacacacaa actgcgttta aaactcttcg gcaagatagt
    28441 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    28501 gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca gcaggatgta
    28561 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg cgaaaccgta
    28621 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    28681 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
    28741 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
    28801 catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    28861 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
    28921 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    28981 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    29041 aaaacacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca gtttacgatg
    29101 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggcgc
    29161 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatacc
    29221 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    29281 accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    29341 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
    29401 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    29461 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    29521 ttggaataaa gtcgccactg atcaaccaaa tatcgcaata aaataagtca cgcgagaacc
    29581 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    29641 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    29701 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    29761 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    29821 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    29881 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    29941 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
    30001 ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt cgttaacggt
    30061 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    30121 gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt ataggaaaca
    30181 aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg cggcgcaagg
    30241 gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat accgcctgca
    30301 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    30361 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    30421 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    30481 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    30541 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    30601 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    30661 tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg acgatcgccg
    30721 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagact
    30781 ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc ggcgatggcg
    30841 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtcg
    30901 ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    30961 accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    31021 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    31081 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    31141 cggactgccc ggcgcgataa agatcgctgc ctaagcccgc cccaacacaa cctgcgtcta
    31201 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccggtg
    31261 gtaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    31321 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    31381 ggcaaacggt catgccgtag cccaccgcgc ggcggatcac ttcactatgg atattgggcg
    31441 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    31501 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctctgct
    31561 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    31621 caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc agcgggagtt
    31681 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    31741 accgccgcca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat
    31801 gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat actggcaacc
    31861 tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcgggg aagtgttccc
    31921 agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgc attaaggccg
    31981 cgctcaaggc cagccgcgaa ggcatcggca gagttttcct gcggcggcaa acctgcgcca
    32041 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    32101 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat aacataaaga
    32161 gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg catcacattg
    32221 tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    32281 gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg agcaactttc
    32341 cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattttc ttcacgccag
    32401 tcggtcgtga cttctgctaa cactgcagcc ggagattttc cgttcaggcg cgtgacgcct
    32461 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    32521 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    32581 tcgagctggc aatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    32641 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    32701 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    32761 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    32821 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    32881 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    32941 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    33001 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc ccgcgataac
    33061 gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg atgatgttgc
    33121 gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggcgag ccaggtacgt
    33181 atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt ttattgagag
    33241 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    33301 gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgcgcgcg taatgtagta
    33361 caaacttata ttattgcgct acaatttaga tcaaaaaaag aacaacacat aaaaaaatga
    33421 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    33481 tatgctttca acggcttagc tactcgttta aaggattaat catgaagttg aattttaagg
    33541 gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc tgtatctcgt
    33601 atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    33661 ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat gtttttgtag
    33721 gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag atgctcaatg
    33781 atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt gttttaaatg
    33841 cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat aacgaagaag
    33901 aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt accaggtcac
    33961 tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac tcaaaggtga
    34021 tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc agaggcattc
    34081 cggatggtgt ttccgcagca ttactgccag tgactgttac actggacatc attactgcac
    34141 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    34201 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    34261 ccatcttcaa ggttggattt tgtgacaaag ttcgccactt ctttcaccga aggaatagcg
    34321 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg
    34381 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc
    34441 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    34501 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    34561 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    34621 aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct gatatcgcgg
    34681 ttagcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    34741 agtttttcca ggaaacgata gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca
    34801 tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc
    34861 atatgcagct ctttcaggta gttgtgcaca cctgcatacg gacgacccgt cgttagcacg
    34921 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    34981 tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    35041 tcccaaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    35101 cacccgaggg aaaaggggat gcaacgcggg ttgcatgctc cccatttgct tagcggaaga
    35161 gtttcccttt cagcagttcc atgcctgcgg aaagcagatc gttattggct tgtgctgaca
    35221 cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc agtaaactgg
    35281 aagccgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga
    35341 gcgccgactc cagttgctcg ccgctaactg attgatttct ctgttgatta ctcaaccagg
    35401 ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccac
    35461 cctgctcctc aacccaactt aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg
    35521 caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc
    35581 caaaaagtat agatctgcgg gatgataatt gcggattgca gaaataaaaa gggcggagat
    35641 gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct tacccggcct
    35701 acaaaatcat gcaaaatcaa tatattgcag gagtctcgta ggactaataa gcgtagcgca
    35761 tcaggcacgc tcgcatggtt aacgccatta aatatcaata ttcgccgctt tcagggcgtt
    35821 ctcttcaata aacgcacgac gcggttctac ggcgtcgccc atcagcgtgg tgaacaactg
    35881 gtcagcggca atcgcatcct taacggtaac gcgcagcata cgacggcttt ccgggtccat
    35941 ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac gctggatgga
    36001 gaggccacga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg
    36061 ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac gcagtttctc
    36121 acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat ccagcggata
    36181 gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc tcgaacaggt tttgttcggc
    36241 attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt tcagttcgct
    36301 gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gaaaggtcag cttccgtcaa
    36361 cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct ccatgcggtt
    36421 gatcattttc tgcgtcgcgt tgtactcaga taccagtttc tctaacgctt cgccagccaa
    36481 tgccggtgca ctggcgttgg tgtgcagcgt tgcgccgtcc agcgcgatag agatctggta
    36541 ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc ttgcctttct tcactttgta
    36601 cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca tctgacgata
    36661 gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat
    36721 gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga taccacagcc
    36781 aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga agcgcgcttt
    36841 ctcgacgtta aggattttac ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc
    36901 ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg aaagcgccgg
    36961 atcgcgttcc tggcagtctg ccagtttgcc cggcaggcca gctaagtcga gcgcaccttt
    37021 acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag catcgataat
    37081 tttgccaacc acgattttcg catcagttgg gttttccagc aggtattccg ccagcagttc
    37141 gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa accagtttgt ctttggtctg
    37201 ggaggagaat ttcgggtccg gcacttttac ggaaacgacc gcaatcaggc cttcacgcgc
    37261 atcgtcaccg gtggcgctga ctttggcttt tttgctgtag ccttctttgt ccatgtaggc
    37321 attcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc cgtcacgctg
    37381 cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc actgcagcgc
    37441 cacttcaacg ccaataccgt ctttttcggt ggagaagtag aagatatttg ggtggatcgg
    37501 cgttttgttc ttgttcagat attcaacgaa cgctttgatg ccgccttcat agtggaagtg
    37561 gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa acgccggagt tgaggaacga
    37621 caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat tggtgaaggt
    37681 ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc cggtaaccgc
    37741 cagcggagcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac cctcgcgctg
    37801 aataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa cgccgtgcag
    37861 accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca gaacggtcat
    37921 gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc
    37981 gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt ctttacagtg
    38041 acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt ggtgcagacc
    38101 ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg catccagccc
    38161 tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt ctcgctcatt
    38221 tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc gaattttcgt
    38281 ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt gattgcgtcg
    38341 cttttaagcg gctggcaagc aacccgcgac gctcatcatc aagctcagag gcaaaatcat
    38401 ctatcaggta gagacaccgc cgcccgcttt cacgagtgag gaactctcct tgcgccagac
    38461 gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc accggcgcac
    38521 cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag gttagctggc
    38581 gatcgcgttc aaaattacgt tccagcacct cagcgtactc tgtctctttc tcccagccgc
    38641 gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaggta tcatccatat
    38701 cggccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc gccagcggga
    38761 tcagttcttt atcccacggg cgtagctgtt cgtaacgagt cacctggcgc agcgccgcat
    38821 tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatcca ggttcgttgt
    38881 gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg agtaaagtaa
    38941 acccttctgg cgttatcaac tgcatcggca tcaggtgcgc cagttccgcg actttatgcc
    39001 cgtccgtacc gtcaatacgg actttgctgt cgccctgttt gtctttggtt aagccaatcg
    39061 ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaatgcctcc tgctcatggc
    39121 gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc gtatagatgg
    39181 cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta aagccgggag
    39241 ataaagcgag atccgcggtt tcaatattgc ggaaatcgcg gatcaacaag cgggtgaggg
    39301 acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg catcttcaat
    39361 ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcgcatt tcagcgcgtt
    39421 cagaacatcc agcacatagc tgacgttgaa gccgatttcc atctccgcac cgctataggt
    39481 aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcagtaa ttttcagctg
    39541 gttttcgctg acatacaggc gcacaccgcg gaatttctcg ttagagagaa tcgccgcgcg
    39601 agcaaacgcc tgcttgagca gatcgcagcc tgcttccaga tgtttgtccg ggttcttcgg
    39661 cagaacgcga cggtaatccg ggaagcgacc atccaccagt ttcgaggtga aaataaagtc
    39721 gccaacgtgg gcacgaatgt tattgctgcc aatctgcaca cgcagcagat tgtcgccgcc
    39781 gtcgagcata cgcatcagtt caatcacgcc tttacgcggt acgatcaccg aatggcttgg
    39841 caaagattga ccaattggca ttgaacagac cgccagacgg tggccgtcgg ttgccacggt
    39901 gcgcagttct tcaccttcgg tttcaaacag cataccgttt aagtaatagc gaacatcctg
    39961 atgcgccatc gaaaactggg ttgcttcaat cagacgcttc atcgtcgcct gcggcagggt
    40021 aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg gcagggtaga
    40081 cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca gctgcacggc
    40141 aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg ccggaacggt
    40201 cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt ccatctcgag
    40261 atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat taccgagaat
    40321 cggtagcgta ggacgaccac ctaacggacc gctcacctgt tgtagcggtt ttaataaatg
    40381 ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt aaatttgaaa
    40441 aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg caggcatgaa
    40501 gcaccgtcgt gtggtcacgc ccaccaaacg catcgccaat ctctggcaga ctgtggttag
    40561 tcagctcttt cgctagcgcc atcgccatct ggcgcggacg cgccaccgag cgggatcgac
    40621 gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc ttctgaatat
    40681 tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagcgcc tcacgcacga
    40741 agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg ttcagcgccc
    40801 cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc acttcacccg
    40861 gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacacgg gtttccagct
    40921 ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa cgatcctcaa
    40981 cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga ttaccttcca
    41041 gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta gcaaaaaact
    41101 gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac tcttcgatcg
    41161 cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc atataaacca
    41221 ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc aggtgagttt
    41281 tacccagacc cgtgccgcca taaaggaaca acgggttata ggcaccgcca gggttatccg
    41341 ccacctggca agccgccgcg cgcgccagtt ggttagattt accttcaacg aagttatcaa
    41401 acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc gggacgttat
    41461 cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag
    41521 gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt ttggtgccga
    41581 cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt ccattgatat
    41641 tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg tacagggcca
    41701 gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat tctgtggctg
    41761 gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac acggcggact
    41821 ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt tgacgtacgt
    41881 cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata tgcccgatca
    41941 agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag agatcttctg
    42001 tttctcacag attttcccta tttatccaca ggactttcca gaactcgcta agtgtaaacg
    42061 atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat gaccatagac
    42121 aaaaattggc ttaatcgatc taataaagat ccaggacgat ccttgcgctt tacccatcag
    42181 cccgtataat cctccacccg gcgcgccatg ctggtttcca ctggtgtgag gtcgtacatt
    42241 ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg actccggtgt
    42301 gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc atcgtttcat
    42361 ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag aaatcgccat
    42421 gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg
    42481 tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg
    42541 tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca tttcccaggg
    42601 agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca caacgggctg
    42661 gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc
    42721 ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt aaacgcctga
    42781 cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc gtggtggtgg
    42841 cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg gaaaaattat
    42901 ggcgccgcca ctgtcgcctg gctcgcgggt cctgatagcc ctcattcggg tctatcaacg
    42961 cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt caagctacgg
    43021 aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt
    43081 attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg gaccatttga
    43141 taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc
    43201 gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc tcaggcccaa
    43261 cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc
    43321 agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac catcaacacc
    43381 cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct gaactctacc
    43441 cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca gagcggtctg
    43501 accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta taacgttgaa
    43561 aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc
    43621 gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga ttacgctgtc
    43681 aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc ctcgtttggt
    43741 cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag caacttcgca
    43801 ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga gaaatacaag
    43861 ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg ttgggtggcg
    43921 atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac caacaacttc
    43981 tataccgcta atctgggtaa cggcatcgtc gctatcggct ataaatctca gccggtactg
    44041 gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc ggaaatccag
    44101 gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg ttggttgtgg
    44161 ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt gggtaactgg
    44221 ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc gctgaccaaa
    44281 gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt
    44341 gagcgtctgg gcgatgacaa acagcgtatc agtcaggaaa tgatggcgct gtacaaagct
    44401 gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc aatcttcctg
    44461 gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg
    44521 atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat gggcgtaacg
    44581 atgttcttca ttcagaagat gtcgccgacc actgtgaccg acccgatgca gcagaagatc
    44641 atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc aggtctggta
    44701 ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat ttaccgtggt
    44761 ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg tgagttttcg
    44821 ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggcgga taagcaccgc
    44881 gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat cgtagcccag
    44941 gccacgcctc cgggacgtgg cggcgttggc atcctgcgca tctccggctt caaagcccgt
    45001 gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga ttatcttccg
    45061 tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt ccctggcccg
    45121 aactcgttca ccggcgaaga tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc
    45181 gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg ccctggtgag
    45241 ttttccgaac gcgcgtttct taacgataaa cttgacttag cccaggccga ggcgattgcc
    45301 gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc gctgcaaggc
    45361 gcattctccg cacgggttaa tcatctggta gaagccctca cccacttgcg catttacgtc
    45421 gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg caaaattgaa
    45481 gcccagctta atgacgtgat ggccgatctc gatgcagtgc gtgctgaagc acgtcagggt
    45541 agtttgttgc gcgaagggat gaaagtagtg attgccggac gtcctaatgc cggtaaatcg
    45601 agcctgttaa acgcgctggc gggccgtgaa gcggcaatcg taaccgatat cgccggaacc
    45661 acgcgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca tatcatcgac
    45721 actgccgggc tacgtgaggc cagtgacgaa gtggaacgta ttggtatcga gcgcgcgtgg
    45781 caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac gacagacgcc
    45841 gttgatccgg cagagatctg gccggaattt atcgcccgtc tgcccgcgaa actgccgatc
    45901 accgtggtac gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag tgaagtgaac
    45961 ggtcacgcgt taattcgtct ctcggcaagg actggtgaag gcgtggacgt gctgcgtaac
    46021 catctcaaac agagcatggg ctttgacacc aatatggaag gcggcttcct ggcgcgccgt
    46081 cgccacttac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa agcgcaactg
    46141 ttgggagcct gggcaggtga actgctggcg gaagagttgc gcctggcaca gcagaactta
    46201 agcgaaatca ccggggaatt tacttcagac gacctgctgg ggcggatttt ctccagcttc
    46261 tgtatt
//
LOCUS       DAJVPT010000020        45210 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVPT010000020 DAJVPT010000000
VERSION     DAJVPT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..223
                     /locus_tag="OQM47_001091"
                     /pseudo
     CDS             1..223
                     /locus_tag="OQM47_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            422..2110
                     /locus_tag="OQM47_001092"
     CDS             422..2110
                     /locus_tag="OQM47_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1479331.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2163..3893
                     /locus_tag="OQM47_001093"
     CDS             2163..3893
                     /locus_tag="OQM47_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479332.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3953..4444)
                     /gene="yajQ"
                     /locus_tag="OQM47_001094"
     CDS             complement(3953..4444)
                     /gene="yajQ"
                     /locus_tag="OQM47_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1479333.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4612..5523
                     /gene="panE"
                     /locus_tag="OQM47_001095"
     CDS             4612..5523
                     /gene="panE"
                     /locus_tag="OQM47_001095"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1479334.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5486..6076
                     /gene="yajL"
                     /locus_tag="OQM47_001096"
     CDS             5486..6076
                     /gene="yajL"
                     /locus_tag="OQM47_001096"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1479335.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6130..7578)
                     /gene="thiI"
                     /locus_tag="OQM47_001097"
     CDS             complement(6130..7578)
                     /gene="thiI"
                     /locus_tag="OQM47_001097"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1479336.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7784..8026
                     /gene="xseB"
                     /locus_tag="OQM47_001098"
     CDS             7784..8026
                     /gene="xseB"
                     /locus_tag="OQM47_001098"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1479337.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8026..8925
                     /gene="ispA"
                     /locus_tag="OQM47_001099"
     CDS             8026..8925
                     /gene="ispA"
                     /locus_tag="OQM47_001099"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1479338.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8950..10812
                     /gene="dxs"
                     /locus_tag="OQM47_001100"
     CDS             8950..10812
                     /gene="dxs"
                     /locus_tag="OQM47_001100"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1479339.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10867..11784
                     /gene="yajO"
                     /locus_tag="OQM47_001101"
     CDS             10867..11784
                     /gene="yajO"
                     /locus_tag="OQM47_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1479340.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11895..12413)
                     /gene="pgpA"
                     /locus_tag="OQM47_001102"
     CDS             complement(11895..12413)
                     /gene="pgpA"
                     /locus_tag="OQM47_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1479341.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12391..13368)
                     /gene="thiL"
                     /locus_tag="OQM47_001103"
     CDS             complement(12391..13368)
                     /gene="thiL"
                     /locus_tag="OQM47_001103"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1479342.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13446..13865)
                     /gene="nusB"
                     /locus_tag="OQM47_001104"
     CDS             complement(13446..13865)
                     /gene="nusB"
                     /locus_tag="OQM47_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1479343.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13885..14355)
                     /gene="ribE"
                     /locus_tag="OQM47_001105"
     CDS             complement(13885..14355)
                     /gene="ribE"
                     /locus_tag="OQM47_001105"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1479344.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14444..15547)
                     /gene="ribD"
                     /locus_tag="OQM47_001106"
     CDS             complement(14444..15547)
                     /gene="ribD"
                     /locus_tag="OQM47_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1479345.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15551..16000)
                     /gene="nrdR"
                     /locus_tag="OQM47_001107"
     CDS             complement(15551..16000)
                     /gene="nrdR"
                     /locus_tag="OQM47_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1479346.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16151..16690
                     /locus_tag="OQM47_001108"
     CDS             16151..16690
                     /locus_tag="OQM47_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1479347.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16988..17872
                     /gene="tsx"
                     /locus_tag="OQM47_001109"
     CDS             16988..17872
                     /gene="tsx"
                     /locus_tag="OQM47_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1479348.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18049..18396)
                     /locus_tag="OQM47_001110"
     CDS             complement(18049..18396)
                     /locus_tag="OQM47_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1479349.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18525..19496)
                     /gene="secF"
                     /locus_tag="OQM47_001111"
     CDS             complement(18525..19496)
                     /gene="secF"
                     /locus_tag="OQM47_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1479350.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19507..21354)
                     /gene="secD"
                     /locus_tag="OQM47_001112"
     CDS             complement(19507..21354)
                     /gene="secD"
                     /locus_tag="OQM47_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1479351.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21382..21714)
                     /gene="yajC"
                     /locus_tag="OQM47_001113"
     CDS             complement(21382..21714)
                     /gene="yajC"
                     /locus_tag="OQM47_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1479352.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21737..22864)
                     /gene="tgt"
                     /locus_tag="OQM47_001114"
     CDS             complement(21737..22864)
                     /gene="tgt"
                     /locus_tag="OQM47_001114"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1479353.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22919..23989)
                     /gene="queA"
                     /locus_tag="OQM47_001115"
     CDS             complement(22919..23989)
                     /gene="queA"
                     /locus_tag="OQM47_001115"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1479354.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24082..24655
                     /gene="acpH"
                     /locus_tag="OQM47_001116"
                     /pseudo
     CDS             24082..24655
                     /gene="acpH"
                     /locus_tag="OQM47_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24660..26477)
                     /gene="malZ"
                     /locus_tag="OQM47_001117"
     CDS             complement(24660..26477)
                     /gene="malZ"
                     /locus_tag="OQM47_001117"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1479355.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26633..28006)
                     /gene="proY"
                     /locus_tag="OQM47_001118"
     CDS             complement(26633..28006)
                     /gene="proY"
                     /locus_tag="OQM47_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1479356.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28082..29401)
                     /gene="brnQ"
                     /locus_tag="OQM47_001119"
     CDS             complement(28082..29401)
                     /gene="brnQ"
                     /locus_tag="OQM47_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1479357.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29808..31103)
                     /gene="phoR"
                     /locus_tag="OQM47_001120"
     CDS             complement(29808..31103)
                     /gene="phoR"
                     /locus_tag="OQM47_001120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1479358.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31161..31850)
                     /gene="phoB"
                     /locus_tag="OQM47_001121"
     CDS             complement(31161..31850)
                     /gene="phoB"
                     /locus_tag="OQM47_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1479359.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32040..33242
                     /gene="sbcD"
                     /locus_tag="OQM47_001122"
     CDS             32040..33242
                     /gene="sbcD"
                     /locus_tag="OQM47_001122"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1479360.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33239..36382
                     /gene="sbcC"
                     /locus_tag="OQM47_001123"
     CDS             33239..36382
                     /gene="sbcC"
                     /locus_tag="OQM47_001123"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1479361.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36508..37689
                     /gene="araJ"
                     /locus_tag="OQM47_001124"
     CDS             36508..37689
                     /gene="araJ"
                     /locus_tag="OQM47_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1479362.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37832..38740)
                     /gene="mak"
                     /locus_tag="OQM47_001125"
     CDS             complement(37832..38740)
                     /gene="mak"
                     /locus_tag="OQM47_001125"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1479363.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38865..39776
                     /gene="rdgC"
                     /locus_tag="OQM47_001126"
     CDS             38865..39776
                     /gene="rdgC"
                     /locus_tag="OQM47_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1479364.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39854..40309)
                     /locus_tag="OQM47_001127"
     CDS             complement(39854..40309)
                     /locus_tag="OQM47_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479365.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40306..40641)
                     /locus_tag="OQM47_001128"
                     /pseudo
     CDS             complement(40306..40641)
                     /locus_tag="OQM47_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40796..41080)
                     /gene="ppnP"
                     /locus_tag="OQM47_001129"
     CDS             complement(40796..41080)
                     /gene="ppnP"
                     /locus_tag="OQM47_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1479366.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41152..41829)
                     /locus_tag="OQM47_001130"
     CDS             complement(41152..41829)
                     /locus_tag="OQM47_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1479367.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42087..42278)
                     /gene="yaiA"
                     /locus_tag="OQM47_001131"
     CDS             complement(42087..42278)
                     /gene="yaiA"
                     /locus_tag="OQM47_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1479368.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42328..42852)
                     /gene="aroL"
                     /locus_tag="OQM47_001132"
     CDS             complement(42328..42852)
                     /gene="aroL"
                     /locus_tag="OQM47_001132"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1479369.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43035..43493)
                     /locus_tag="OQM47_001133"
     CDS             complement(43035..43493)
                     /locus_tag="OQM47_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1479370.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43613..44422
                     /gene="proC"
                     /locus_tag="OQM47_001134"
     CDS             43613..44422
                     /gene="proC"
                     /locus_tag="OQM47_001134"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1479371.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44439..>45210)
                     /gene="adrA"
                     /locus_tag="OQM47_001135"
                     /gene_synonym="dgcC"
     CDS             complement(44439..>45210)
                     /gene="adrA"
                     /locus_tag="OQM47_001135"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1479372.1"
                     /translation="MGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLE
                     LTGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTG
                     VYNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQL
                     QITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISV
                     GVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11742 a        11798 c        11075 g        10595 t
ORIGIN      
        1 cagttcatat gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat
       61 cgatcatgtt gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga
      121 tcgtttttat ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga
      181 atcaatggat attactgaca aagcctatgc cgacaaatta aaaaaattag aaactccttt
      241 tcttgatgat cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat
      301 gctgacagac ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat
      361 gtctactgta tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt
      421 tattcaatct cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta
      481 tatctcatca actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga
      541 cacagataac ctctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg
      601 aaagataaaa aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag
      661 ttattacaat agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa
      721 cggtgagttt attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa
      781 cgacaaaatt aaagatggtg aatgccctca tttttatgaa gatgggaaaa tcaaacagca
      841 acatagctat ctgaatagta aactcgatgg tcctgcatat gaatattatc ctgatggcaa
      901 actcatgcag gaaaatttct atcagcaaag tgtgttgatt ggaaacgata ccagttacta
      961 ccaatccgga aaaataagct ctatacacaa tagaaattcc cgtggtcagt atgatggagc
     1021 taacgaacgt tatagtgaag agggtaagtt actgtctaaa tccgtctata aagacggaaa
     1081 acaaatttct gttcaaacct ggtatgaaaa tgggcagaaa gaagaggaga aacattttga
     1141 tgagcaaggc caattaaatg gtctggttaa gcaatggtat aaaaatggta atcttgcaaa
     1201 atcacaaaac tataaacatg acatactcga tggtaacagt gaagagtggt acgaaaatgg
     1261 catccctgaa agtttgtacc cttataagaa tggaaaaacc gacggcattg ccaaatcatg
     1321 gaataagtat ggtaaattaa cctattcaat cgaatacaaa aatggcgtag agaatggggc
     1381 atatcgtaac tggagcaaaa acacaggtaa actaaccaaa gaaaccctgt acgtcaatgg
     1441 tatcagacag ggagtggaaa aagagtttaa cgatcgcaca ggtaaactat taacctccac
     1501 gcaatatgtt aataataaaa gacatggtac ggaggaaact tatgatcaaa acggaataaa
     1561 atatattaca tgctatcaaa atgatcagaa attatcgtct ttagacaatc caacacaaat
     1621 aaaagataat gcaacaacag gcgattccag tgctcaattt gcattaggga aatacgaatt
     1681 catctgtgca aatatagatg aagggattaa atggttaacc aaatctgcag agcaaaaaaa
     1741 cactgatgcg atatactttt tagctacagc ctacaaaggc aatggtatac ctgcaaacaa
     1801 tgaaaaatac ctagcatatc tgcaacaagc tgcaacactg ggcagtagta atgcccaggc
     1861 ggaaattggc tatttatatc tcatcggaaa agaactacca cagaatcttc ctgatgctgg
     1921 tgtctggttc aaaaaagcag ctgcacaagg taattttgcc gcacatttct atctgggaag
     1981 gatgtatcag aatggtgacg gcgttgagag aaatatggaa aaagccaggt ttcatctatc
     2041 taatgcggca gaaggtggaa tagaacctgc gcttaaagca ttaaatgaac ttgaacatca
     2101 aacaaaataa agcgctcagg gaccacgtaa ggttcatcaa acagaaaagg atatagtttg
     2161 cgatgaaagc gaaatccact ttgttattaa tgattttatc tgttttttat tctcatcagg
     2221 ttttatccag cgaacccact cagccggatg tttattctgt cgtcgagaaa aaactaaact
     2281 ctgctttacc tctggaagaa aataccacct tcagatcgca ggctgaatgg tttaatttgc
     2341 aatatgaatt attagtatct ggctaccctg aacgtgctta caatttactg agcaaacttg
     2401 agacagaatc gaaaaacgct aaatattatc tggatttaac tgcctccaga gtcgctgaag
     2461 aagaagcacc agagacagcc ttaacttttt tatcaaaaat tgggttaaat aaaccggcta
     2521 ctcttacatc ctttgagagc tatatcaata gctggataaa aagcaaacaa cccgaaaagg
     2581 ctatgcagct tctcagcctc gatgagaatg ccctgaatta ttatttaccg actgtgttag
     2641 tcgcatatca gatcgttcct gataaggcaa taagtattta taattctgta tatggagata
     2701 atgttgttat accctatcag caagtcacca tgttacttac agttgcggag ggttatcaaa
     2761 caaaaggcga tataaaaaac gccattcatt atgccgataa ggcgctgaat atgtttgata
     2821 aagctattgc tgtagatagc agtcgtgaat cccattatag tgatgagtat ttgaaactca
     2881 tgaatatcta cgccgccaat ggcaacaaag aaaaagcggt ggttttatct caacgtctac
     2941 acaaagccat taccgaatct caatcgaatt acttaagtac tctggaagga attttattgt
     3001 tttatagaaa taatgatatg cagcaagact atcaaaacag cctttcaaat tacatagtat
     3061 ttatcgataa aatttttgcc ttttccccat caacaagaag tgaattaagc ctcattaatt
     3121 tattaaattc attaaatgag gtggaattga tgagaaaacg tctggcgttt ctgatgacag
     3181 caccagaata cgcttgttat aatagtcagt attgttatga agacaaagtc agcgcattaa
     3241 aaatcctgta tcgtcatcat gacaatgagc taatggacaa atatttcacg actcttctta
     3301 aagagatcag taatcaacca ttagaagact gggatataat aataagtaat actggtgaaa
     3361 aatttagtga agtgggtcta accaaatatg ctcaacatct cgcatctttc gctgaagaat
     3421 tttatataaa agagcaaaaa tcacattctg aaagtgaaat gagaggacta ttttcatcac
     3481 ttgccgaatt atatagtttt ggtaatgata ctgccagcgc aaaccgagtg ttccatcagc
     3541 atgtccctgc cttcaataca gatcatatga ttcaatattt tataaatgcc aaagaatgga
     3601 acaatgccag ggaattattg ataaaagagg aaatgttggg tatgcataat ctgatattgt
     3661 tggagaatat ttgtatgcaa aaaaatgcag aatgcatggc acacattacc tttacgctta
     3721 ataaattaac aacacaacca gcaataacaa aaatagatag cgtgggtaat gagcaacttt
     3781 atcagattgg caaaatctac cataagttgg aaattaaacc tgagccagag caacaggttt
     3841 taattcagtc gctttatgac cgggcatcag gaagcgcctc tgcaactcaa taaatgcatg
     3901 gtcaaaaggg ccaccacggt ggccctgaca atcagtagct atatccagaa agttaatcgc
     3961 ggaagttttt gaactggaac ggctgaccga gatcgccacc gcgcaccatc gccatgacag
     4021 cctgcaaatc atcacgagat ttgccagtca cgcggatctc atcgccctga atttgcgcct
     4081 gcactttcag tttgctgtct ttgatcatct tgacgatttt tttctgagtg gcgctctcaa
     4141 tgccctgttt cagtttcgct tccacaaacc aggttttacc gctatgaacg atattttccg
     4201 gtacatccag tgaactgcct tcaatgccgc gcttcagcag cttggcacgc agaatatcca
     4261 gcaactgatt gacctggaag tcggactcgc tcaacacttt gatggttttg ctggcgtcgt
     4321 tcagctcaaa tgaggcttca acgttacgga agtcaaaacg ggactccact tcgcgactcg
     4381 cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc agagacaata tcgaaagatg
     4441 gcatcttttc ctctcccttc atttttgatg cgaagcataa tacctgcaaa gttaaataac
     4501 caacagtcct gcaccaggac gtatccctta attgtgcata gtcgcaacag catgcacaat
     4561 taagggatag ccttattgaa cacccggagt ggttgcgggt gaggaggaac aatgaaaatt
     4621 accgtattgg gatgcggtgc cttagggcaa ttatggctta cagcactttg taaacagggt
     4681 catgaagttc agggctggct gcgtgtaccg caaccttatt gtagtgtgaa tctggttgag
     4741 acagatggtt cgatatttaa cgaatcgctg accgccaacg atcccgattt tctcgccacc
     4801 agcgatctgc tcctggtgac gctgaaagca tggcaggttt ccgatgccgt caaaagcctc
     4861 gcatccacac tgcctgtaac tacgccaata ctgttaattc acaacggcat gggcaccatc
     4921 gaagaattgc aaaacattca gcagccatta ctgatgggca ccaccaccca tgccgcccgc
     4981 cgcgacggca atgtcattat tcatgtggca aacggaatca cgcatatcgg tccggcacgg
     5041 caacaggatg gcgattacag ttatctggcg gatattttgc aaaccgtgtt gcctgacgtt
     5101 gcctggcata acaatattcg cgccgagctg tggcgcaagc tggccgtcaa ctgcgtgatt
     5161 aatccactga ctgccatctg gaattgcccg aacggtgaat tacgtcatca tccgcaagaa
     5221 attatgcaaa tatgcgaaga agtcgcggca gtgatcgaac gcgaagggca tcatacttca
     5281 gcggaagatt tgcgtgatta cgtgatgcag gtgattgatg ccacagcaga aaatatctcg
     5341 tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa tcgactatat caatggtttt
     5401 ctcttacgcc gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa
     5461 atggtaaaaa gaaaggagag tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg
     5521 tagtgaagag actgaagccg tcaccacgat caacctcttg gttcgcggcg gtatcaaagt
     5581 caccactgcc agcgtcgaca gcgatggcaa cctggcaatt acctgctcgc gcggcgtgaa
     5641 gctgctggcg gatgcgccgc tggtcgaagt ggctgatggc gaatatgacg tgatcgtgct
     5701 gcctggtggc attaaaggca cggagtgttt tcgcgatagc actctgctgg ttgaaaccgt
     5761 taaacagttc caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt
     5821 gctggtgccg cacgatatct ttccgattgg caatatgacc ggcttcccga cgctgaaaga
     5881 caaaattccc gctgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt
     5941 gctgaccagc caggggccgg gtacagctat cgactttggt ctgaaaatta tcgacctgct
     6001 ggttgggcgc gagaaagccc atgaagtggc atcacaactg gtgatggcgg cagggattta
     6061 taattattac gagtagtgtc ggatgcggca aacgtcgcat ctgaccagat gcgacgtaaa
     6121 aactacaaat tacgggcgat ataccttcac attgttaaag ccttgctcgc gcagatagag
     6181 cgcctgcaga cggctcatca ccccgcgctc acaccacagc agccaggttt tgttctggtc
     6241 gagatcgcca aatttggtgc tcagtttata gaacggcaga gaaaccacat caatcccttc
     6301 gactttcagt ggcttatctt cctgttcatc gacagaacgg atatcgagaa tcacgtcgtt
     6361 cgggccgaag ccgttgacgg tttccacttc caccacttcc tgctcggtct gctgggcgat
     6421 ttcgcggata tcaacgttat tcgcttcctc aaccacttta tcgagaatac tgaagtcgaa
     6481 cttctcttct tccgcttcaa tcttcgattt aaccgctttc accgtcgggc ttttggagat
     6541 cacgccacaa tattccggca tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag
     6601 gttgatgatg tgctctttgt cgtaagaaat cagcggacgc aggatcagcg tgtcagagac
     6661 gttatcaatc aggcgcaggt tggtcagcgt ctgactggac acctggccga gcgcttcgcc
     6721 ggtgaccagc gcctgtacgc cgtaacgttc agccacttta gatgcggcac gcaccatcat
     6781 acgtttgagg ataacgccca tctgaccgtc gtcgattttc tcgagaattt ccccgacaac
     6841 cggttcgaaa ttaatggcga caaaacgcac gcggtgggag ctgccaaagc ggttccacag
     6901 ataatgcgcc acctgacgca cgccaatttc atgcgctgcg ccgccgaggt taaagaagca
     6961 gtaatgcacg cggcagccgc gacgcatcaa catataactg gaaacaccgg agtcgaaacc
     7021 accggaaatg agcgacagca catcttcctg ggtgccaatc gggaaaccac caataccttc
     7081 gtagcgacct ttaatcagca ggaggcgatc gtcttccact tccagatgaa cagttacatc
     7141 cgggttagtc agcttcacgc gcgcggattc aatatgctga tttaaaccgc cgccgacgta
     7201 acgttccaca tcaatcgagc taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa
     7261 ggttttgcct tccagctgat cgcgatactg aaccaacgct ttctcaaaaa tatcgtgcat
     7321 gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg atacccggaa tacgggtcag
     7381 ggcgtcgcga atagtcagac gctggttttc atctttcgcg cgaacttcga tgttatccca
     7441 gtggcggacg acagcgagcg tctcatcata gtgctttaaa acgttacgaa tgttcccggt
     7501 aaggattttt ataaagcgca agcgcacaga ttggcttttg atggtgattt ccgggaacaa
     7561 tttaatgata aacttcatgg cggcaatggt tcgttggcaa gccttaagcg acttgtatag
     7621 ggaaaaatac agcagcccac acctgcggct gcatccaggc gcggaagtat accactaaca
     7681 tcgctttgct gcgcacatca ccttaccatt gcgcgttatt tgctatttgc cctgagtccg
     7741 ttaccatgac ggggcgaaaa atattgagag tcagacattc attatgccga agaaaaatga
     7801 ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa cagattgtaa cccgtctgga
     7861 aagtggcgac ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg tgcagctggc
     7921 acgtcagggg caggccaaat tacaacaagc cgaacagcgc gtacaaattc tgctgtctga
     7981 caatgaagac gcctctctaa ccccttttac accggacaat gagtaatgga ctttccgcag
     8041 caactcgaag cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg
     8101 ccctttcaga acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag
     8161 cgcctgcgac ctttcctggt ttatgccacc ggtcatatgt ttggcgttag cacaaacacg
     8221 ctggacgcac ccgctgctgc cgtagagtgt atccacgctt actcattaat tcatgatgat
     8281 ttaccggcga tggatgatga cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt
     8341 ggcgaagcaa acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgattcta
     8401 agcgatgcca atatgccgga agtgtcggat cgcgacagaa tttcgatgat ttctgaactg
     8461 gcgagcgcca gcggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa
     8521 ggcaaacacg tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg
     8581 attcgcgccg ccgttcgcct tggtgcatta agcgccggag ataaagggcg tcgtgctctg
     8641 ccagtactcg acaagtacgc agagagcatc ggccttgcct tccaggttca ggatgacatc
     8701 ctggatgtgg taggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt
     8761 ggtaaaagta cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat
     8821 ctgatcgacg atgcccgtca ggcgctgaaa caactggctg aacagtcact cgatacctcg
     8881 gcactggaag cgctagcgga ctacatcatc cagcgtaata aataaacaat aagtattaat
     8941 aggcccctga tgagttttga tattgccaaa tacccgaccc tggcactggt cgactccacc
     9001 caggagttac gactgttgcc gaaagagagt ttaccgaaac tctgcgacga actgcgccgc
     9061 tatttactcg acagcgtgag ccgttccagc gggcacttcg cctccgggct gggcacggtc
     9121 gaactgaccg tggcgctgca ctatgtctac aacaccccat ttgaccaatt gatttgggat
     9181 gtgggacatc aggcttatcc gcataaaatt ttgaccggac gccgcgacaa aatcggcacc
     9241 atccgtcaga aaggcggtct gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta
     9301 ttaagcgtcg ggcattcatc aacctccatc agtgccggaa ttggtattgc ggttgctgcc
     9361 gagaaagaag gcaaaaatcg ccgcaccgtc tgtgtgattg gcgacggcgc gattaccgct
     9421 ggcatggcgt ttgaagcgat gaatcacgcg ggcgatattc gtcctgatat gctggtggtc
     9481 ctcaacgaca atgaaatgtc gatttccgaa aatgttggcg cgcttaacaa ccatctggct
     9541 cagctgcttt ccggtaagct ttactcttca ctgcgcgaag gcgggaaaaa agttttctct
     9601 ggcgtgccgc ccattaaaga gctgctcaaa cgtaccgaag aacatattaa aggcatggtg
     9661 gtgcctggca cgttgtttga agagctggga tttaactaca tcggcccggt ggacggtcac
     9721 gatgtgctgg ggcttatcac cacgctaaag aacatgcgcg acctgaaagg cccgcagttc
     9781 ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga cccgatcact
     9841 ttccacgccg tgcctaaatt tgatccctcc agcggttgtt tgccgaaaag tagcggcggt
     9901 ttgccgagct attcaaaaat ctttggcgac tggttgtgcg aaacggcagc gaaagacaac
     9961 aagctgatgg cgattactcc ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt
    10021 aaattcccgg atcgttactt cgacgtggct attgccgagc aacacgcagt gacctttgcc
    10081 gccggtctgg cgattggtgg atacaaaccc attgtcgcga tctactccac cttcctgcaa
    10141 cgcgcctatg atcaggtgct gcatgacgtg gcaattcaaa agctaccggt cctgttcgcc
    10201 attgaccgcg cgggcattgt tggtgctgac ggtcaaactc accagggcgc ttttgatctc
    10261 tcttacctgc gctgcatacc agaaatggtc attatgaccc cgagcgatga aaacgaatgt
    10321 cgccagatgc tctataccgg ctatcactat aacgatggcc cgtccgcggt gcgctacccg
    10381 cgcggcaacg cggttggcgt ggaactgacg ccgctggaaa aactaccaat tggcaaaggc
    10441 attgtgaagc gtcgtggcga gaaactggtg atccttaact ttggtacgct gatgccagaa
    10501 acggccaaag tcgccgaatc gctgaacgct acgctggtcg atatgcgttt tgtgaaacca
    10561 cttgatgaag cgttaattct ggaaatggcc gccagccatg aagcgctggt caccgtagaa
    10621 gaaaacgcca ttatgggcgg cgcaggtagc ggagtgaacg aagtgctgat ggcccatcgt
    10681 aaaccagtac ccgtgctgaa cattggcctg ccggacttct ttattccgca aggaactcag
    10741 gaagaaatgc gcgccgaact cggcctcgat gccgccggta tggaagccaa aatcaaggcc
    10801 tggctggcat aatccctact ccactcctgc tatgcttaag aaattattca tggcaggagt
    10861 ggcagcatgc aatacaaccc cttaggaaaa accgaccttc gcgtttcccg actttgcctc
    10921 ggctgtatga cctttggcga gccagatcgc ggtaatcacg catggacact gccggaagag
    10981 agcagccgtc ccataattaa acgcgcgctg gaaggtggca taaatttctt tgataccgcc
    11041 aatagctatt ccgatggtag cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc
    11101 cgtcgtgaag acgtagtcgt tgccaccaaa gtgttccatc gcgttggtga tttaccggaa
    11161 ggattatccc gtgcacaaat tttgcgctct atcaacgaca gcctgcgacg tctcggcatg
    11221 gattatgtcg atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg
    11281 ctggaagccc tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc
    11341 atgcacgctt cgcagtttgc tcaggcgctg gaactacaaa aacagcactg ctgggcgcag
    11401 tttgtcagta tgcaggatca ctataatctg atttatcgcg aagaagagcg cgagatgctg
    11461 cctctgtgtt atcaggaggg cgtggcggtg attccgtgga gcccgctggc gcgggggcga
    11521 ctgacgcgtc catggggaga aactaccgca cgactggtgt ctgatgaggt ggggaaaaat
    11581 ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt
    11641 gaagaactcg gtgcaacacg agcacaagtt gcgctggcct ggttgttaag taaaccgggc
    11701 attgccgcac cgattatcgg aacttcgcgg gaagaacagc ttgatgagct gctgaacgcg
    11761 gtggatatca ctttgaagcc ataacagatt gccgagctgg aaacgccgta taaaccgcat
    11821 gcggtcgtag gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag
    11881 gcatcggtgc acaactacga cagaataccc agcggccagt gatgaccgat aaagtacagg
    11941 atgcctgcgg aaatcacccc ggcgacaata tcgtcgatca tgatccccat gccgccatgt
    12001 acattgcgat caaaccagcg gatcggccac ggcttccaca tatccagaat acggaaaatc
    12061 acaaacccgg cggcaaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac
    12121 ataccaataa attcgtccca gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc
    12181 gtttgatgac aaagatagac gccgatacag atccccaaca tcaccaccag cgagtagagc
    12241 tgccagggta aaaaggtcat cagataccag aaagggatcg ccgccagcga tcccatcgag
    12301 ccaggaacga ttgggcttaa tccacttccg aatccgacag caagcagatg ccacggatta
    12361 ctcatcttca ggcgactttt cgcgacatct ttatggcgtg acaaaatggt catatccttt
    12421 ccagtcaaac gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt
    12481 catttgcccg atacaggtaa acggtacgcc aaggtgtccg agagcaacat ccagcgcgcc
    12541 acggttcagt tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag agagcgccca
    12601 gcgcagcgcc tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatgc
    12661 caggtcaata cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag
    12721 accgtcagag agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat
    12781 acgcggcgat ggacggagat gacgtttgat caagtagtca gcatctttag catcggcaac
    12841 ctgcaaacgg ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg taccggtcac
    12901 atagatccag tcacccggtt tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa
    12961 gccgtggata cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg
    13021 catatcgtaa taattgagaa gatcaaacaa actgtcgctg aaggactcaa gccacgcttc
    13081 gtctacgtcc ggtaaggtta atgccagcgt cagccaggcc ggatcggccc ccatcgctgc
    13141 cagatcgctt aggttcaccg ccagtgcttt ataagccaga tcagcaggat cgatatcagg
    13201 gaggaaatgg ttgcccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg
    13261 gatattgaga agagcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct
    13321 tacacggtca aaataacggg caatcaggga gaactcgcca catgccatac gttatgcctc
    13381 agcaggtaaa aaagaaaagg ccggcgatca tggaacagtc ttccgtgaat ctaccggcct
    13441 ggatattact ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg
    13501 aacttatggc tgtcttctgc gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc
    13561 actttgtatg gcacatcact acgtttagac agttcgtaca gcgcaatgcg cagtactgct
    13621 ttttctacct ggcccagttc ttccagcagg cgggacaggt agggcttcat cagtccgtcg
    13681 aggtatgcgg tgttagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg
    13741 tcttttacat cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac
    13801 aactgccagg agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt
    13861 ttcacggatt tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc
    13921 aagcgcggtc agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat
    13981 cgcttgttca atgctttcag tggtcagaac cccaaaagca accggaattt cgctgtcctg
    14041 ggcaacatgc gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc
    14101 accacgaata accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc
    14161 acccgccgcc agcggcagct cataggcacc aggcacccaa acaacggtaa tgttttcatc
    14221 ttttacctga ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat
    14281 aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc
    14341 ttcaataatg ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt
    14401 tagcataata tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat
    14461 gcaggcaaac atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg
    14521 ctaatttctc aagccctggc agcgagcata atccacgggc gtcgctgcct aatagtttag
    14581 gtgcgatata gacaatcagc tcatcgacta aacccgcctg tagcaatacg ccagcgagcg
    14641 ttggccccgc ttccacccag atgctgttaa tctgctgttt acccagttgc atcatcagta
    14701 caaccagatc cagatgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc
    14761 actcacgaga atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc
    14821 gatgttccgg cgtcacgcga ttttggctat caatcacaat acgtactggc tggcggagat
    14881 tttgttgcgg gtagagcgcc tgagtttgtt catccagttc agaccaacgc actgttaagg
    14941 ctggatcatc cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgtgcgcgct
    15001 gtcgttgtac gtcgcgccgc gcctgaggcg aagtgatcca ctggctttcg ccgctcgcca
    15061 tcgccgtgcg gccatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc
    15121 gcatccgctt gagaaagcct ttattcaatt gctcggcttc actcatcatc agcccgtggc
    15181 tgacgtcaat gccagcctgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag
    15241 gatcttgcat cgctgcgacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg
    15301 gtggcgtacg gccatgatgg ctacagggtt cgagtgtcac atacgccgtt gcgcctttgg
    15361 ctttttcacc cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gctcgttggt
    15421 ggtaaccttc accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg
    15481 ggtgggtggt aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt
    15541 cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga
    15601 aactgcggta gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct
    15661 ccatcaccag attgccaatc atcttgctcg gtacttcgcg ctcaccggtg gcgcgcagct
    15721 gcgatttaat atgattgatt gccatttcaa cgtaatcgga actcaccgga cgtttttcca
    15781 gcgcccgcag cattccgcta cgcaatttct cttcattaaa cggttcacgc acgtcgttgc
    15841 tttttacaac acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac
    15901 acaccagaca ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa
    15961 ttaccttagt gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg
    16021 ttaacggaaa tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga
    16081 taaaggatcg atgctttcgc tgcttttacc ctctaccatt aggctaattg cgacgagaaa
    16141 gggacgacct atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct
    16201 tagcgcctgt gtgcagcaaa gtgaagtgcg acagatgaaa cacagcgtca gcacgctgaa
    16261 ccaggagatg acgcaactca atcaagaaac agtcaaaatc actcagcaaa acaggctgaa
    16321 tgcaaaatcc agcagtgggg tttaccttct gcctggagcg aaaacaccgg caagactgga
    16381 aagccagatc ggtactttac gtatgtcgct ggtgaatatt acgcctgata cagatggcac
    16441 cacccttaca ctacgtattc agggtgagtc gaatgatccc ttgcccgcat tcagcggaac
    16501 tgttgaatat gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcagaa
    16561 tcaattgatt aatgcccctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca
    16621 attaaagggg atttctgtgg atcagttagg ctttgtacgt atccacgaca ttcaacctgt
    16681 tatgcagtaa acgtatttcg ggacgatttt gtgcgtcccg caacatcttc cccgtcattt
    16741 tgttactctg cttacatcac ctggattgat agtaaaagtt tgcaacaagg gcgaaagtca
    16801 gtacaatccc cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa
    16861 ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg
    16921 aaatatttag aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg
    16981 catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt
    17041 tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag
    17101 cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta
    17161 ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc
    17221 gccggtattc ttcggcggta actccgatgc taaaggtatc tggaaccacg gttctccgct
    17281 gtttatggaa atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt
    17341 cggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa
    17401 agatggtcgc cagagcacct ggtacatggg tctgggtacc gatatcgaca ctggcctgcc
    17461 gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa
    17521 cgaaaacgag tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct
    17581 gtggggcggt cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg
    17641 ggatgacagc ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc
    17701 cagccatatt ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg
    17761 gcacgacggt ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa
    17821 cgttcgctct accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa
    17881 atgccgggat ttattcccgg catttctgat tgttaacaac gtgtgctttg ttcatgccgg
    17941 atgcggcgta aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa
    18001 taacgtaggc ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt
    18061 gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg
    18121 cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg
    18181 ctcatgatcg tggcaataga gacacaacaa ttcccagtta ctgccatctt ccgggttatt
    18241 ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc aggttagaat aaacaaactc
    18301 gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct
    18361 ttctaaccgt gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta
    18421 gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca tcaaaaaaaa
    18481 tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattaagg cagaattgac
    18541 ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg cttcataccc
    18601 agtttcagag ccaacgcaga tgctacgtag atggaagatg cagtgccgat ggaaacaccg
    18661 ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagagata cagcatcagg
    18721 ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac
    18781 acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc acgaattcgg
    18841 tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa cgatgccaca
    18901 atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat gataacgtcg
    18961 tgcgtcagcg caataaccac ccctgccgcc agtcgccact caaagcggaa acctacgtac
    19021 acgaggatag acagcagcgc tgccatcaac gccatcgcac cggtttgcgc aaggtcagcc
    19081 cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt ggtggattcg
    19141 ttaatcacct tcagaacttg gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc
    19201 ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg ctcttcaaaa
    19261 ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc gagcgtaatt
    19321 tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac gcccataata
    19381 acgatagcag cgattaacag cagaccagag atgccgaaag cccagtagtc ccagcgcata
    19441 aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg tgccacatcg
    19501 cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt ttacgatggc
    19561 acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg tagtaatcgc
    19621 gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa tcagcgtggt
    19681 gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa ttgcctgttg
    19741 aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta cgttcgcatc
    19801 gaccgctacc gcaagggtta agacgatacc cgcgatacca ggcatactca gtgttgcgcc
    19861 tggcaacagc gacataatac cgacgatcaa gatcaagttg gcaatcagag cactggtcgc
    19921 aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca gcagaccggc
    19981 aaggcaagcc tccagcccct gctcaatgtt ctgcataccc agggtcgggc caattgtacg
    20041 ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg
    20101 acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca gacgagactg
    20161 gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac catttgcatc
    20221 tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc cgatgttgtc
    20281 cttggtgaag ttagacataa tgttgccacc agcgctatcg agcgagatgt taacctgcgg
    20341 ctggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccag tcagaattac
    20401 gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg
    20461 tacgcgaccg gatgccgcag cggcctgatc aacattggtg tttaccagac ggaattccag
    20521 cgttgccgtc gcgcccagaa tctctttcgc acgcgcagtg tcctgaatac cgggcagttc
    20581 aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc caagttggtt
    20641 tacacggtta cgcaggatat taatgttctg ctgcaccgca tattcacgcg cttcactcag
    20701 acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctgctaa tcaccaggtc
    20761 cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat cgcggaaagt
    20821 gatgctcagg ccgtagttgt tttctttacg aacagtggta tacgggatgc ccttttcgcg
    20881 caggtcactg cgtaggctat cgatattttg ttcctggagt ttgccaagcg cggtgtccat
    20941 atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct tcatcggctc
    21001 agcatgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg ccacgacgta
    21061 tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc aactgggtgt cagtggagtc
    21121 gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta ttttttcttc
    21181 ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc
    21241 agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca gcagaccgat
    21301 gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta acacggcaat
    21361 tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca ggacggcagc
    21421 tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag caatgtagcc
    21481 gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa cttcatcacc
    21541 tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct gtggacgcag
    21601 gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag acatcgggct
    21661 accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt
    21721 tccctcatta ttaatattaa tcaacgttca aaggtggtac ttctcgcccc tgacgctggt
    21781 aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt aaacccgcca
    21841 tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt
    21901 tgcaacggtc gagatgatgc aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac
    21961 actcaggatc gagtgggcca gtatcgctct tatatttcgc attgcggatt ttcaccacgc
    22021 catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg cagtcaaaca
    22081 tatcgatacc gcgacgtacg ccttcaacca ggtcttccgg tttaccaacg cccatcaggt
    22141 aacgcggttt gtctgccgga atttgcggac acacatgctc cagaatacgg tgcatatctg
    22201 ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaagccg atatctacca
    22261 gacctttaac agatatatca cgtaaatctt cgtaaatact gccctgaatt ataccaaata
    22321 acgcattttt gttaccaaga ctgtcaaaac gctcacggct acgcttcgcc caacgcagag
    22381 acatctccat agagcgtttt gcgtaatccc agtcagcagg atacggcgta cactcatcaa
    22441 agatcatgac gatatccgaa ccaagatcgt actgaatctc catcgacttt tccgggtcga
    22501 ggaaaatcgg gtcgccgttg atcgggttac ggaagtgaac gccctgttcg gtgattttac
    22561 gaatatcacc aaggctgaag acctggaagc cgccggagtc ggtaagaatc ggtcctttcc
    22621 actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc
    22681 acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact tcttccggcg
    22741 tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt tccactacgc
    22801 cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt
    22861 tcattttttc tccactacgt cagaaaaaca gtccaacgtt taaaccagcg ccgcggaatt
    22921 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    22981 aactaaaaaa gcgatatttc tcttctaccg ccgctttata ggcattcatg gtgtgttgat
    23041 aaccggcaaa ggccgaaacc agcataatca gcgtcgactc tggcaagtgg aagttcgtca
    23101 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    23161 agaacggttc gataagatcg ttttttgccg cctgtgccgc gctttccagc gaacgtactg
    23221 aagtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    23281 ccacatcctg cggtacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    23341 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    23401 ctttggcgcg taatttttcc agcaaaggct cgtcaaaatg cagaccagcg gtcggggctg
    23461 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    23521 ccggacgatc gatatacggc ggcagcggca tatggccgat gctgttgagg atatccagta
    23581 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    23641 cgttgatact ttcgtcatcg cccagcaaca gttctgcgcc aggttttggc gctttcgagg
    23701 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ctcaaccagc acttcaatct
    23761 tgccgccgct ggctttacgc ccaaacagac gcgccgggat cacgcgggta ttattaaaaa
    23821 ccagaagatc gccggggttg agcttatcga gtaaatcggt gaaagtaccg tgcgtcagcg
    23881 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    23941 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    24001 agactaaaat aagagtcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    24061 cctttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    24121 gaaagctcgc tttctggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    24181 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtattgactg acaatctgcc
    24241 ggaagtccgc gaagcacagg agtggtttcg tagtgaaacg cgccgcgttg cgcctattac
    24301 gctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt
    24361 tccgctacag gaatttgtct gttatgcccg cgagcaagtg atgacgattt tgccggactc
    24421 accgccacgt tttatcaatc tgaacaatta cttgtggtca gagcagtggc tggtgcgcta
    24481 tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc gcccacgtct
    24541 ggatgccctg cgtgactcct ggtacgattt agacgctcat tatgacgccc tcgaaacccg
    24601 cttctggcag ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt tataaaacgt
    24661 tagttcatcc ataccgtagc cgaaatggca ggcaaagcca gaatcccgtc agtcagttgc
    24721 ccatgccctt ctttgcattg ccattgcacg gcattgagaa acggtgacgc gggtagcacc
    24781 acttcactgg cctcgccacg gttgattgcc accagtacac gttgctgatt cagcacgcgg
    24841 acaaatacca ctacgttatc ttccgcatac agcacctgac agccgccacg acgcagcgcc
    24901 tgacttttct tacgcagcac aatcattcgc cggtacagcg cgaataacgc cgtatcctgc
    24961 ttttccacct gccaggggaa cggtttacgg caaaacggat cgtttttacc atccagtccc
    25021 acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca gaccaccgcc
    25081 agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt atcgtggcta
    25141 tcgagctgat taaacatacg taattgttgt tgatgagaaa gccctgcgcg gtaattatcc
    25201 atccaggcca tacaggtttg ggcgtcaatt tgctgcggat cataagagat gtcggtattg
    25261 gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt tcatggcagc atcttccaca
    25321 tcggcctgta accattgccg tgcatcgcca aaatgttcgc cgacaatata cgcttccggc
    25381 tgggtttctt tcgccgcttc ggtgatcccg gcaacgtgct gcatattatt gcgcgcccca
    25441 cccgcctccc ccagcatatg caccacatcc agccgccagc cgtccatatt ccacggcgct
    25501 ttcagccagt ggcggacaat actgtcttcc ccgcgataaa tttcattcac cagactttcc
    25561 gactgataat ccagcttcgt caagctggca tagccaagcc agtcgagcgc cgtgccatca
    25621 tcactaaacg agtaccagtc gcgccagggc gattcagagt tgtgacaagc tccccccgtg
    25681 ccaagattgt gcctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa cacgccgtcc
    25741 agcaccagcc gcattcccag ctgctgcgta ttgtgtcgca aacgcagcaa cgccccatca
    25801 ccgccaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg tacgctggga
    25861 gctttaaaca ccggattgag atacagcgct gtcacgccaa gctttttcag atacggcagt
    25921 ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc ctgcgccgtg
    25981 accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg gtaatagaca
    26041 tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa gatctgataa
    26101 aaaatctgat ccgcagccca ttgtgggccg atatcaggta catcgacggc aaactgctcc
    26161 agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg gcgatcgtgc
    26221 cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc aatcgccgca
    26281 cgccatgcgg tgacgcctgg ctgcggctga ctgcgctgct tatgcattgg tactgacatt
    26341 tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc gcccgtcagc
    26401 cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac cggcaggtgc
    26461 catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc catagcctaa
    26521 tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc ggataacttt
    26581 cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag tattattggt
    26641 tttcagccag ctgacgatcg tggcggcgtt taaacatcca gccaatcaac agcacaacaa
    26701 tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca atcaacccga
    26761 taataaagag caggaaaatc agcccgccga tggtcgttgc taccccaccc ggcactttaa
    26821 atttcagcgc cttaacttct tctggcggca aacggcgacg gaaggcgatt tgcgacagca
    26881 ggatcataat ccacacccat accgtggcga aggttgccag cgaagcgatc accaggaaga
    26941 cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta gtcatcacca
    27001 gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc ggtgcgctgc
    27061 cctgctctgc cataccgtgg agcatacggc ctacgccaaa tacgtcactg ttaattgccg
    27121 acagcgaagc ggtcagcaca acaaagttaa gaatgctggc ggcaaaggta atgcccatat
    27181 gctggaacgt cagtacgaac gggctaccgg cagtgccaac ctgattccac gggtagatag
    27241 acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc acggagttaa
    27301 tcgcacgcgg aatcgatttc tcaggatctt tcgcttcacc ggcggtaatc ccgataattt
    27361 cgatcccacc gtaagcaaac atcaccattt gcaacgacat taccatgcca agccagccgt
    27421 tactgaagaa gccgccgttg ctccacaggt tatgaatacc ggtcggttgc ccgccgttgc
    27481 caatccccca gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg gtggcgactt
    27541 taaagaacga gaaccagaat tccagctcac cgaagacctt cacgctcatc aggtttacgg
    27601 cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc gggaacaaga
    27661 cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg atttcaaaac
    27721 agtaggtcca tccggtaatg taacctgcca gcgggccaag gttttcctgc gcataacgcg
    27781 agaaagagct ggcggccggg ttatgtaccg acatttcccc cagcgcacgc ataatgatat
    27841 acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc attttgatgg
    27901 cgtctgccga accgtaaaac agcccggtgc caattgctga acccagtgcc ataaagcgta
    27961 tgtggcgggt acttagccca cgctttagct tattcttact ttccatcaat tcctaaccca
    28021 tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa tgttcagtga
    28081 tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata atggccagaa
    28141 ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc ggtaaacgct
    28201 gcgcccagga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg agaataccaa
    28261 aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc caccatgagc
    28321 gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc agcaccggta
    28381 cggagatctg gatcagctgg cttaagccga ggttagacac caccatcgag aagccgccga
    28441 ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat tctgcacaag
    28501 cacaggtcag gccaaccgcc gtgaccagac aggcgatgaa gattaacgcc gccagcagga
    28561 agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca gcgccgtttg
    28621 cagactgatc gaccagcgac gcgctgtctg aacccagaca gaacagcgcc aggtacagca
    28681 aagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc agcagacgcg
    28741 cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac accattgcgc
    28801 ccagcgtatc catggtcaga tagccgttaa cgaagccgtt agaaaatgca gcgttttgat
    28861 aagcctcagt cgccgtgctg atagaacccg ccggccagac aatcgcgaca acagacagga
    28921 tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc agcagcttgc
    28981 ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag ataaacagcg
    29041 gcagcgcgga atcacccgtc agcggcgcaa tccccacttc aaaggaaacg gtagctgtac
    29101 gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc agtacgccag
    29161 cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt gccagcgcca
    29221 ctaccgttaa taccggcagg ccaacggcag taatgaggaa gccgaatgcc gcagtccaga
    29281 cgtgttcgcc tgcctgtaaa ccgaccattg gagggaaaat aatgttacct gcgccgacga
    29341 acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat tgatgggtca
    29401 taaatcatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc aatccctggg
    29461 gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc aggcgcggcg
    29521 aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc tgaagcgcaa
    29581 tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg tatatagcga
    29641 taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg ctaatcatgc
    29701 gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt attttgactg caaagaaaat
    29761 aagccagcat cggctggctt atggcaataa aagatgacaa aggcggatta atcgctgttt
    29821 ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc tactgtactc
    29881 tcaatattca ggcgactttc gtgatgattc accgcatgtt tcacgatcgc taaccctaat
    29941 ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg ctcggtcagg
    30001 cgcggaatat gctccggtgc aatgcccggt ccgttatctt caacgctaaa ttcggcaccg
    30061 tgcggcactc gctgccagcg tacggtgata tgcgtgcctt ccggcgtatg attcacggcg
    30121 ttatagacca ggttcgaaat cgcactgcgt agctgatctt cgttgccaga caccttgagg
    30181 ccgttatcta tctcaaaggt aaatgtctgt tttttctgac tcagagtctg agcctcgcgc
    30241 tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaattg cgtcggcgcg
    30301 gcttcaattt tcgacagcgt tagcaactgc ttcaccagcc cttccatccg ctgggtctgc
    30361 tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg ctcatccatc
    30421 atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct cacgttggca
    30481 aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg cgccaccatc
    30541 agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc ggtgttgagc
    30601 accagattga gcgggcgaga aaaatcacgc gttttcagat attgcgtaaa ctccgggtaa
    30661 cgcagtaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc aagaatttgt
    30721 tgcgccagac cgttacacca gaaaataccg ccctcttccg tggtcagcac caccgcgtcg
    30781 ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc acggcggcgt
    30841 tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca gctaccacgc
    30901 cccggtggcg gggtcatact gcgatccacc cacagccacc atgaaaggcg caataaattc
    30961 cagaaatgcc agataagcag tcctgttacc gatgccagca aaaaccaggg caggtaacca
    31021 aaaaatgcac ccaggatgaa agccgggagg cagcaaagta gcagctccag caccagcctt
    31081 ttccacgaca gccgttccag cacgtaagat actccagtta agaaatcata agccctgctc
    31141 tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt accgcgcacg
    31201 gtctgcacca tgcggtcatg cccgccgggc tccagtgctt tacgcaggcg acgaatgtgg
    31261 acatcgaccg tgcggtcttc cacatacacg ttagttcccc agacgtggtt taacagctgc
    31321 tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt aaattctgtc
    31381 ggccccatct ccagcggctc ttcgcccgcc atcactcggt gagatgtcgg gttgagactt
    31441 aatccctgca tcttaatcac ctcttccacc gccattggcg aaatacggcg cattaccgct
    31501 ttgattcgcg ccaccagctc cttcggcgaa aatggcttgg tgatatagtc atccgcgccg
    31561 gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat caccactgga
    31621 atatcccggg tcatcgactc gcgcttgagg tgtttgatga actggatacc ggagccgcca
    31681 ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg attcacagca
    31741 ctgtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac gaagcagacc
    31801 atttcgcgaa ttggagcttc atcttctacg accagaatac gtctcgccat gatttgccct
    31861 gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga tttatgacag
    31921 atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc gcagaaaaaa
    31981 gcaaatggca catctgtttg ggtataatcg cgaccatgct ttttcgccag ggaaccgtta
    32041 tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt aaaagccgcg
    32101 aagctgaaca tcaggctttt cttgactggc tgctggagac agcacaaacc catcaggtgg
    32161 atgcgattat tgttgctggt gatgttttcg ataccggctc gccgcccagt tacgcccgca
    32221 cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg gtggtactgg
    32281 caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg gcgttcctca
    32341 atacaaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt cgcgacggga
    32401 cgccaggcgc agtgctgtgc cccattccgt ttttacgccc gcgtgacatt atttccagcc
    32461 aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt accgattatt
    32521 accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg cccatcatcg
    32581 ccacgggaca tttaacgacc gtgggttcca gtaaaagtga cgccgtgcgt gacatttata
    32641 ttggcacgct ggacgcgttt ccggcacaaa actttccacc agccgactac atcgcgctcg
    32701 ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat tgcggttccc
    32761 ccattccact gagttttgat gaatgcggta agagtaaata tgtccatctg gtgacatttt
    32821 caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaactcaa cccatggcgg
    32881 tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc gatgtatcgc
    32941 aggagccacc tgtctggctg gatatcgaaa tcactactga tgagtatctg catgatattc
    33001 agcgcaaaat ccaggcatta accgaatcat tgcctgtcga agtattgctg gtacgtcgga
    33061 gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc agcgaactca
    33121 gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga cctggatgaa tcgcagcagc
    33181 aacgtctgca gcatcttttc accacgacgt tgcgtaccct cgccggagaa cacgaagcat
    33241 gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat ggaagattga
    33301 tttcacccgc gagccgttcg ccagcaacgg gctgtttgcc attaccggcc caaccggtgc
    33361 ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcacgaaa ctccgcgtct
    33421 ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgctg aatgtctggc
    33481 ggaggtggag tttgaagtga aaggtgaagc gtatcgcgcg ttctggagcc agaatcgggc
    33541 gcgtaaccag ccggacggta atttgcaggt gcctcgcgta gagctggcgc gctgcgccga
    33601 cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa ctgacagcga cgttaaccgg
    33661 gctggattac gggcgcttca cccgttcgat gctgctttcg caggggcaat ttgctgcctt
    33721 cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg gcactgaaat
    33781 ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca ctgagctgga
    33841 gaagctgcaa gcgcaggcca gcggcgtcac gttgctcacg ccggaacaag tgcaatcgct
    33901 gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttaattaccg cgcagcagca
    33961 agaacaacaa tcgctaaact ggttaacgcg tcaggacgaa ttgcagcaag aagccagccg
    34021 ccgtcaacag gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac ctcaactggc
    34081 ggcgcttagt ctggcacaac cggcacgaaa tcttcgtcca cactgggaac gcatcgcaga
    34141 acacagcgcg gcgctggcgc atattcgcca gcagattgaa gaagtaaata ctcgcttaca
    34201 gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg gcgaagcagt cagcagaatt
    34261 acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct tccgtcagtg
    34321 gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg atcgcgagca
    34381 tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaatg cgcttgcggc
    34441 gatcacgttg acgttaaccg ccgatgaagt tgctaccgcc ctggcgcaac atgccgagca
    34501 acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag attgttcccc aacaaaaacg
    34561 tctggcgcag ttaatggtca ctatccagaa tgtcactctg gaacagacgc aacgtaatgt
    34621 cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg ccgatgtgaa
    34681 aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gcccagcgtg cacagttaca
    34741 ggcgggtcag ccttgtccac tttgtggttc caccagccac ccggcggtcg aggcgtatca
    34801 ggcgctggag cctggtgtta atcagtctcg attactggcg ctggaaaacg aagttaaaaa
    34861 gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat gcattaacga agcagcttca
    34921 gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag caagcactta ctcaacaatg
    34981 gcaagccgtc acggccagcc tcaatatcac cttgcagcca caggacgata ttcaaccgtg
    35041 gctggatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac gacatgaatt
    35101 acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc aaattgaaca
    35161 acgtcagcag cagctcttaa cggcattggc gggttatgca ctgacattgc cacaggaaga
    35221 tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc agcaacgcca
    35281 gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acaccgattc tggaaacgtt
    35341 gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata actggcggca
    35401 ggttcatgaa caatgtctcg cattacacag ccagcagcag acgttacagc aacaggatgt
    35461 tctggcggcg caaagtctgc aaaaagccca agcgcagttt gacaccgcgc tacaggccag
    35521 cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa cactaacgca
    35581 gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa cgctggtcac
    35641 tcagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt tggctctcac
    35701 tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt tgcgtgaaaa
    35761 caccacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata accgtcagca
    35821 acaacaaacc ttaatgcagc aaattgctca aatgacgcag caggttgagg actggggata
    35881 tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg cccaggggct
    35941 gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc acgtgcgcta
    36001 tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata cctggcaggc
    36061 agatgcggta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg ttagtctggc
    36121 gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact cgctgttcct
    36181 tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg atgcgctgga
    36241 tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgtagaag cgatgaaaga
    36301 gcgtattccg gtgcagatca aagtgaaaaa gatcaacggc ctgggctaca gcaaactgga
    36361 aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg ggtgaattat
    36421 ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac ggtgattggt
    36481 agccgttctg gtggttgtga tggtggtatg aaaaaagtca ttttatcttt ggctctgggc
    36541 acgtttggtt tggggatggc cgaatttggc attatgagcg tgctcacgga gctggcgcat
    36601 aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc actggtggtg
    36661 gtggtcggtg cgccaatcat cgcactcttt tccagccgct actcactcaa acatatcttg
    36721 ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc ttcgtcttac
    36781 ctgatgctcg ccattggtcg gctggtatcc ggctttccgc atggcgcatt ttttggcgtc
    36841 ggagcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc cgtggcgggg
    36901 atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac gtatttaagt
    36961 caggaatgct ggcgttacac ctttttattg atcgctgttt ttaatattgc ggtgatggca
    37021 tcggtctatt tttgggtgcc agatattcgc gacgaggcga aaggaaatct gcgcgaacaa
    37081 tttcactttt tgcgcagccc ggccccgtgg ttaattttcg ccgccacgat gtttggcaac
    37141 gcaggtgtgt ttgcctggtt cagctacgta aagccataca tgatgtttat ttccggtttt
    37201 tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt gctgggaaat
    37261 atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc agtgactgac
    37321 tttatcattg tactggcact gctgatgctc tttttctgcg gcggcatgaa aacaacgtcg
    37381 cttatttttg cttttatttg ttgcgcggga ttatttgccc tttcagcacc tctgcaaata
    37441 ttgttactgc aaaatgccaa aggaggagag ttattaggtg ccgcaggtgg gcaaatagcg
    37501 tttaacctcg gtagcgccgt cggcgcatat tgcggaggta tgatgctgac gctggggctg
    37561 gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat gtcatcgttg
    37621 ctgctgtatg gtcgctataa gcgccagcaa gcggcggata gtccggtgct ggcgaaacca
    37681 ctggggtaga ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg tgaacgcctt
    37741 atccgcccta cgcggttctg gcacattttg taggcctgat aagacgcggc aagcgtcgca
    37801 tcaggcatga tgcgccaatt gcctacgttt tttactcttg tggccataac cacgcagcgc
    37861 cgcgtacgcc gctggaatca ccgtgcttcg ccttactcac cggcgtttca cattcgccgc
    37921 cgaagacaaa ttgtttaatc aactgcccaa ccgtttgata taaacggtct acattgctca
    37981 tcccgccccc caggacaatc acatccggat cgagaatatt cacgacatgt gccagcgatt
    38041 ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg ctttcttcaa
    38101 ccaggcggat aatttcactg cctttcagcg catgtccgct caaacgacga taatccgtcg
    38161 cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa taacaaggga
    38221 cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg tagcggattg tgtccccact
    38281 cacctgccgt gccattgccg ccgatatgcg tccgcccatt gaatgccacg cccgcgccgc
    38341 atcccgtgcc gataatcacg gcaaataccg tctgcgctcc cgctgccgcg ccatctactg
    38401 cttctgaaac cgccagacag ttggcatcat ttgccagccg cacttcccgc tgcaacctcg
    38461 cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattgaca ttcttcacca
    38521 caccggtgta gggcgaaatc gaacctggaa tgcccatacc taccgttccg cgctgccctg
    38581 tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtctgc cggtaatcat
    38641 cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc agtgcaatca
    38701 cttcagtttt ggtgccgcct aaatcgatac ctatacgcac tgtactctcc ttattttttt
    38761 caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc cgacaattcg
    38821 ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat tatcatgctg tggttcaaaa
    38881 atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag atggaaaaac
    38941 agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag atgggctggg
    39001 ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt caaattgtta
    39061 tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt gattaaacag gcgctggaag
    39121 cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc gaaaaagatt
    39181 cgctgaaaga cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt tttagccaga
    39241 caatgatgtg gctcgacacg gttaacggtt tgattatggt ggactgcgcc agtgccaaaa
    39301 aagcggaaga tacgctggca ttactgcgta aaagcctggg gtcgttaccg gttgtaccat
    39361 tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc ggtagtgcgg
    39421 cacagggctt ccagctgctt gatgaagccg agctgaaatc gttgctggaa gatggcggcg
    39481 tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac attgaagccg
    39541 gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt gtgatgtgcg
    39601 acgatggttc gctcaagcgt cttaagttct gcgacgagct gcgcgatcaa aacgaagata
    39661 tcgaccgtga agatttcgcc cagcgttttg atgccgattt catcctgatg actggtgaac
    39721 tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa cgttaattgc
    39781 tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt acgaagccac
    39841 accaggcata taattattcg ctacggcgag caataatttt tagcgcagca atattatgcg
    39901 ttttacgccg taactgatct aatcgcctca tccctattcg cctgtcagca gcacgcagaa
    39961 tattcactaa cccccattta tcgctggtaa attgttttgt taacagctcc gcttttgggg
    40021 tatcaatata ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg taaggatacc
    40081 agcaagtttc accgtgaaaa ttacgcactc cagcgttacc tgcaataaag tctttcgggt
    40141 aatcccagat cacgttttta tccaacgtga tccaataacg cggtaaatcg gtacttccac
    40201 cattttggct gcgcatggga taacgggtac agtgaatttg caggttgatc gttggcgtca
    40261 gtaagtcata gatctcccgc tgtaacttgc tccatggacg ttgtgtcatt gtttttcctc
    40321 aagccggtga cgcgcttcac tgcgaatttt ccgcgttgcc ccggttttca ctaaatcacg
    40381 taactgcgaa agatcgggtt gttcgacaaa taagagtaat gatggatcct cttttaacca
    40441 cgccgttttc acatcggcag ccagctgtgg attattaata gccagcgacc gatcctgagg
    40501 ctcagtcaat gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg ccgtttggcg
    40561 caaatgatca ttgcgacttt gcagcaatgc acgccaccat tcatcatctg atttatcttt
    40621 tatcgtagcc agttccgcaa aaaatacctg gagatcttca ctgatattta tatcaacctt
    40681 gatgaattat gaatatatcg gaaattgaaa aaatcgcagc aaaggaaata ttattatggg
    40741 ttaaagagag gatatttcag aaaaatgccc tcccgttcaa ggggaaggcg aggaattaca
    40801 gatagcggca cagataagag gtaggttcgg caacttgaag atgaaactca ctgtgaccgg
    40861 gaacattaaa caccgaaccg gcttcataca cttgccagtc ggtcgcgtca ggcagtaaca
    40921 cattcagcgc gccactgatt accgtcatct cttccggctc agcggtgctg aaggtgtatt
    40981 cgccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa aagccgatcg
    41041 atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc tgtctggtag
    41101 ttgaacatct acttcactat aggggccaga ggcgctggct gtcacgcaaa attacaccag
    41161 taattccgca gccagccgtg caatcaatac gttagagagc aagacaggaa catcgagctg
    41221 tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa caatccagca tgatgacatc
    41281 agcccctttt gccagtaatt ctttcccagc atcaatgatt ttttgttctg aatcatgaat
    41341 ggggttaccc aatgaaaata ccggcgattt ctgcaaaatt tgccattttt gcgcctgaac
    41401 gggcagcatc tcctcaaccg gaacgataac ccccacctga tgatcttcaa caatagagga
    41461 aaccagtgga ggcaatattc gcgacggctc aagaaagatc gtattacgcg cagtcatact
    41521 actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat cgagcacttc
    41581 aaccacacct tgcagatctc gctccacttt acgacgcgaa acatgggcca gctggttgtc
    41641 atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg ccatcacttc
    41701 ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt caatgtattc
    41761 cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg atggtcagga tcgccaacga
    41821 cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg caagtgtgaa
    41881 gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag cagacgtgca
    41941 cggcataatg taaagattcc aaatgattcc agtaatggaa tgttattgtt taatattcta
    42001 attattagaa aaacataaat tatgaaaaat gtgacgcaga tcccttgtag tggcactcag
    42061 aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata gcgttttttc
    42121 gcatccatcg cttcctgagc cgtcggatgt tcactgatca acgagtctgg tttaggatga
    42181 tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttttc aatcgtcact
    42241 atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta tttcacggga
    42301 tgaacgttaa gtataggcgc tctaaaatca acaattgatc gtctgtgcca gggcgctacg
    42361 aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat gcgcaacttc
    42421 gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca gcggttttcc
    42481 cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa ccaggactga
    42541 tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac gattaaattc
    42601 cgtcagaata atgccgccgc ctgtagcgat aacggtggat gccgcagtta ccgcttccag
    42661 cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga tctccgcgac
    42721 cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac ggttaagcga
    42781 atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga tcagaaaaag
    42841 aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc cggtgtcatg
    42901 agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa aattccactt
    42961 aaagtaaaaa tctcaataca tcccttacta taccaataaa tattcaagaa tgaagtgtaa
    43021 ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt ctccagctcc
    43081 gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt ctggatccca
    43141 ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat ggtcgctggc
    43201 gtgtaacgtt cgccgcgcga attaagcgcc gctgcgcctt tctcgatggc ttcagcagcc
    43261 aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac aatttcgtta
    43321 tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga tggcggcacg
    43381 cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc cgcgcgatac
    43441 aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt cataaagtca
    43501 tcctttgtta ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata gttctgttta
    43561 tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg caatggaaaa
    43621 gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg gtctgattgc
    43681 cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccgg ataaagtcgc
    43741 cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag tggcgcaaat
    43801 cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc ttagcgaaat
    43861 cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg tcacgctcga
    43921 ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc cgaacactcc
    43981 cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa ccccagaaga
    44041 taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa ttgctgagcc
    44101 gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtat ttatgtttat
    44161 cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg cgtataaatt
    44221 tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag aacatccggg
    44281 ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg tacgcgtact
    44341 ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta tggaaaaatc
    44401 agaaaaactc agcaaatcct gatgactttc gccggacgtc aggccgccac ttcggtgcgg
    44461 ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa ccactcacga
    44521 tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa agttacctgt
    44581 ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat ggcggtaatg
    44641 gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc aaaccgacca
    44701 atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca
    44761 tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc
    44821 agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt
    44881 tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gagtactcat gacctgcaac
    44941 ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacatcaga
    45001 ggataaacga caataatggg aagggagagc caccattcca gcggagcact attgaacgac
    45061 acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc
    45121 gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacagaat catcaacata
    45181 gcggtggaag gcagcacgtt tacgcccatt
//
LOCUS       DAJVPT010000021        43480 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVPT010000021 DAJVPT010000000
VERSION     DAJVPT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            10..84
                     /locus_tag="OQM47_001136"
     tRNA            10..84
                     /locus_tag="OQM47_001136"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Gln,seq:ctg)
     gene            complement(238..1413)
                     /gene="ubiF"
                     /locus_tag="OQM47_001137"
     CDS             complement(238..1413)
                     /gene="ubiF"
                     /locus_tag="OQM47_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1479373.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1559..2983
                     /gene="miaB"
                     /locus_tag="OQM47_001138"
     CDS             1559..2983
                     /gene="miaB"
                     /locus_tag="OQM47_001138"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1479374.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3136..4176
                     /locus_tag="OQM47_001139"
     CDS             3136..4176
                     /locus_tag="OQM47_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1479375.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4173..4640
                     /gene="ybeY"
                     /locus_tag="OQM47_001140"
     CDS             4173..4640
                     /gene="ybeY"
                     /locus_tag="OQM47_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1479376.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4730..5608
                     /gene="corC"
                     /locus_tag="OQM47_001141"
     CDS             4730..5608
                     /gene="corC"
                     /locus_tag="OQM47_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1479377.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5633..7171
                     /gene="lnt"
                     /locus_tag="OQM47_001142"
     CDS             5633..7171
                     /gene="lnt"
                     /locus_tag="OQM47_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1479378.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7568..8476
                     /gene="gltI"
                     /locus_tag="OQM47_001143"
     CDS             7568..8476
                     /gene="gltI"
                     /locus_tag="OQM47_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1479379.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8646..9386
                     /gene="gltJ"
                     /locus_tag="OQM47_001144"
     CDS             8646..9386
                     /gene="gltJ"
                     /locus_tag="OQM47_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1479380.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9386..10060
                     /gene="gltK"
                     /locus_tag="OQM47_001145"
     CDS             9386..10060
                     /gene="gltK"
                     /locus_tag="OQM47_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1479381.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10060..10785
                     /gene="gltL"
                     /locus_tag="OQM47_001146"
     CDS             10060..10785
                     /gene="gltL"
                     /locus_tag="OQM47_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1479382.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10903..11838
                     /gene="rihA"
                     /locus_tag="OQM47_001147"
     CDS             10903..11838
                     /gene="rihA"
                     /locus_tag="OQM47_001147"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1479383.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11922..13592
                     /gene="hscC"
                     /locus_tag="OQM47_001148"
                     /pseudo
     CDS             11922..13592
                     /gene="hscC"
                     /locus_tag="OQM47_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13652..15103)
                     /gene="djlC"
                     /locus_tag="OQM47_001149"
     CDS             complement(13652..15103)
                     /gene="djlC"
                     /locus_tag="OQM47_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1479384.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15100..15807)
                     /locus_tag="OQM47_001150"
     CDS             complement(15100..15807)
                     /locus_tag="OQM47_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1479385.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15909..16463
                     /locus_tag="OQM47_001151"
     CDS             15909..16463
                     /locus_tag="OQM47_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1479386.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16473..17900)
                     /locus_tag="OQM47_001152"
     CDS             complement(16473..17900)
                     /locus_tag="OQM47_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1479387.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17897..18604)
                     /locus_tag="OQM47_001153"
     CDS             complement(17897..18604)
                     /locus_tag="OQM47_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1479388.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18768..19745
                     /locus_tag="OQM47_001154"
     CDS             18768..19745
                     /locus_tag="OQM47_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1479389.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19815..20297)
                     /locus_tag="OQM47_001155"
     CDS             complement(19815..20297)
                     /locus_tag="OQM47_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1479390.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20532..23114
                     /gene="leuS"
                     /locus_tag="OQM47_001156"
     CDS             20532..23114
                     /gene="leuS"
                     /locus_tag="OQM47_001156"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1479391.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23129..23710
                     /gene="lptE"
                     /locus_tag="OQM47_001157"
     CDS             23129..23710
                     /gene="lptE"
                     /locus_tag="OQM47_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1479392.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23710..24741
                     /gene="holA"
                     /locus_tag="OQM47_001158"
     CDS             23710..24741
                     /gene="holA"
                     /locus_tag="OQM47_001158"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1479393.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24743..25384
                     /gene="nadD"
                     /locus_tag="OQM47_001159"
     CDS             24743..25384
                     /gene="nadD"
                     /locus_tag="OQM47_001159"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1479394.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25408..26019
                     /locus_tag="OQM47_001160"
     CDS             25408..26019
                     /locus_tag="OQM47_001160"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1479395.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26279..26596
                     /gene="rsfS"
                     /locus_tag="OQM47_001161"
                     /gene_synonym="rsfA"
     CDS             26279..26596
                     /gene="rsfS"
                     /locus_tag="OQM47_001161"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1479396.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26600..27067
                     /gene="rlmH"
                     /locus_tag="OQM47_001162"
     CDS             26600..27067
                     /gene="rlmH"
                     /locus_tag="OQM47_001162"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1479397.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27098..28999
                     /gene="mrdA"
                     /locus_tag="OQM47_001163"
     CDS             27098..28999
                     /gene="mrdA"
                     /locus_tag="OQM47_001163"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1479398.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29002..30114
                     /gene="mrdB"
                     /locus_tag="OQM47_001164"
                     /gene_synonym="rodA"
     CDS             29002..30114
                     /gene="mrdB"
                     /locus_tag="OQM47_001164"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1479399.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30125..31213
                     /gene="rlpA"
                     /locus_tag="OQM47_001165"
     CDS             30125..31213
                     /gene="rlpA"
                     /locus_tag="OQM47_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1479400.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31352..32563
                     /gene="dacA"
                     /locus_tag="OQM47_001166"
     CDS             31352..32563
                     /gene="dacA"
                     /locus_tag="OQM47_001166"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1479401.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32674..32937
                     /locus_tag="OQM47_001167"
     CDS             32674..32937
                     /locus_tag="OQM47_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1479402.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33038..33679
                     /gene="lipB"
                     /locus_tag="OQM47_001168"
     CDS             33038..33679
                     /gene="lipB"
                     /locus_tag="OQM47_001168"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1479403.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33938..34891
                     /locus_tag="OQM47_001169"
     CDS             33938..34891
                     /locus_tag="OQM47_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1479404.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35100..36065
                     /gene="lipA"
                     /locus_tag="OQM47_001170"
     CDS             35100..36065
                     /gene="lipA"
                     /locus_tag="OQM47_001170"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1479405.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36166..36369)
                     /gene="tatE"
                     /locus_tag="OQM47_001171"
     CDS             complement(36166..36369)
                     /gene="tatE"
                     /locus_tag="OQM47_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1479406.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36498..37286)
                     /locus_tag="OQM47_001172"
     CDS             complement(36498..37286)
                     /locus_tag="OQM47_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1479407.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37379..37762
                     /gene="crcB"
                     /locus_tag="OQM47_001173"
     CDS             37379..37762
                     /gene="crcB"
                     /locus_tag="OQM47_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1479408.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37816..38025)
                     /gene="cspE"
                     /locus_tag="OQM47_001174"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37816..38025)
                     /gene="cspE"
                     /locus_tag="OQM47_001174"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1479409.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38200..38760)
                     /gene="pagP"
                     /locus_tag="OQM47_001175"
     CDS             complement(38200..38760)
                     /gene="pagP"
                     /locus_tag="OQM47_001175"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1479410.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39349..40734
                     /gene="dcuC"
                     /locus_tag="OQM47_001176"
     CDS             39349..40734
                     /gene="dcuC"
                     /locus_tag="OQM47_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1479411.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40775..41116)
                     /locus_tag="OQM47_001177"
                     /pseudo
     CDS             complement(40775..41116)
                     /locus_tag="OQM47_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41454..41963)
                     /locus_tag="OQM47_001178"
     CDS             complement(41454..41963)
                     /locus_tag="OQM47_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1479412.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42126..42371
                     /locus_tag="OQM47_001179"
     CDS             42126..42371
                     /locus_tag="OQM47_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1479413.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42346..>43480
                     /locus_tag="OQM47_001180"
     CDS             42346..>43480
                     /locus_tag="OQM47_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1479414.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSV"
BASE COUNT        10933 a        10895 c        10830 g        10822 t
ORIGIN      
        1 gcacccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
       61 gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt
      121 tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc
      181 gacgctgccg cgtcttatcc gacctacggc tggcacgcat ccggcaatgt tgtaaggcta
      241 caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat
      301 taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa
      361 cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt
      421 gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca gaacatcaat
      481 cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt
      541 atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg
      601 cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg
      661 cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc gaatacgcgt
      721 cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgcgg
      781 tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct
      841 aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg
      901 cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg tttcaccgcc
      961 cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg aggttggcac
     1021 acgtaacgtt actttcggat gcgcttccag cgcctgccac aacgcctgtt gcaggactgt
     1081 gttttccacc atgtagccaa gcaacggcaa ctttaattcg gctgcgtcaa ataccacatg
     1141 tgccgtttcc cactcccagg tttccagtct gcggtaagga tggcaacgca tagcctgtac
     1201 tgcatcccag acccctaacc ctttaagcaa tgataccgaa gccgcgctga tcgccgagat
     1261 ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt
     1321 taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca ttcctccgcc
     1381 gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct
     1441 ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt cacaacggca
     1501 gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat
     1561 gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa
     1621 gatggccgat ctgctggatg cctcccacgg ctatcaactg accgacgtgg cggaagaagc
     1681 ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca
     1741 tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg
     1801 tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat
     1861 tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga
     1921 ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctgccgga
     1981 accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg
     2041 cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat
     2101 tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca
     2161 gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc accggatcgt ttgccgatct
     2221 gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc
     2281 gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc tggtgagctt
     2341 cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg gacgtaccca
     2401 tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cggatattca
     2461 gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact tcgagaaaac
     2521 gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg
     2581 tccaggaacg cctgccgccg atatggttga tgatgttccg gaagaagaga agaagcagcg
     2641 tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gtcggatgct
     2701 cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc
     2761 cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa
     2821 attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg
     2881 aactgaagat gaaatgggtc tgcgcatggc agaaacaccg gagtcggtga ttgcccgcac
     2941 ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag gcctgccgtt
     3001 ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt
     3061 tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg gcggcataca
     3121 gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaat
     3181 gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt
     3241 ctcggcatcg agatcaatcg ccgcgataac cactttaaac tgaccggccg tccgatttgc
     3301 gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atactgcccc gatgcgcggt
     3361 cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc gcgggtactg
     3421 gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aactaaacgc
     3481 ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat tctcgaccat
     3541 gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc agtggctgcg
     3601 gcagttgatg ccctggagcg tcaggaaatt cgccgtattc tgctgactcg tccggcggtc
     3661 gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat
     3721 ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt cgagaaactg
     3781 attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac
     3841 gacgcgttta tcattctcga tgagagccag aacactacca tcgaacagat gaagatgttc
     3901 ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac
     3961 ctgccgcgta atactaaatc aggcttacgt catgccattg aagtgctggc cgatgtcgaa
     4021 gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcacccggt ggtggcgcgt
     4081 atcgttaacg cttatgaagc ctgggaagaa gcagaacaaa aacgtaaagc ggcgctggca
     4141 gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac
     4201 tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg
     4261 cggtgatccc gcagtttcag gaagagtcgg aagtgactat tcgcgtggtc gataccgccg
     4321 aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct
     4381 tcccgtttga agtgccgcct ggcatggaga tgtcgctact gggcgatctg gttatctgcc
     4441 gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc
     4501 atatggtggt gcacggcagt ctgcatttgt taggttacga tcacattgaa gatgacgaag
     4561 cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt
     4621 acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt
     4681 gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga
     4741 caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag
     4801 ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc
     4861 cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat
     4921 cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg
     4981 caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc
     5041 ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc
     5101 gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt
     5161 cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca
     5221 catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat
     5281 cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg atatcgactt
     5341 ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg aagacttcaa
     5401 cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg gtctggtgat
     5461 gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt
     5521 caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa tcccggatga
     5581 ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact acatggattt
     5641 tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg
     5701 cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt cgctgatggg
     5761 gcttcaggcg ttgaccttta accgtcgccc actccagtct gccgctattg gcttttgctg
     5821 gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg
     5881 cgggatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct
     5941 gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg
     6001 cgtggcgatt gccacccctg ctctctggca agtgaccgag tttctgcgcg gttgggtgct
     6061 gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct
     6121 ggcaccgcta atgggcgtgg aagccattaa cttcctgctg atgatggtta gtggcctgct
     6181 ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc
     6241 cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca
     6301 ggtttcgatg gttcagggcg atattccgca atcgctgaaa tgggacgaag gccagcttct
     6361 taatacgcta aagatttact acaacgcaac ggcaccgctg atgggcaaat catcgttgat
     6421 tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc
     6481 actggacggt gagttgcgtg ataaaggtag ctcgctggta accgggattg tcgacgcgcg
     6541 tctcaataag cagaaccgct acgataccta caacaccatc atcacgctgg gtaaaggtgc
     6601 accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc cgtttggcga
     6661 gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc
     6721 gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gcaaatggta ttgagcttac
     6781 tgcggctatt tgctacgaga ttattctcgc cgagcaagtg cgcgataact tccgcccgga
     6841 taccgactac ctgctgacta tctccaacga tgcgtggttt ggtaaatcta ttggtccatg
     6901 gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag
     6961 caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg cgatgatccc
     7021 gcagttcgcc cgcgaggtgt taaccactaa cgtgacgccg accaccggac tcacaccata
     7081 cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt ttgctgctgt
     7141 gttgatgagt ctgcgtcagc gacgtaaata atcccttctt atcgtgccgg aatacatccc
     7201 atatttcggc acgctcaccg gatgcggcat cagtgcatta tccggataac aatattctcc
     7261 tatccaactt tggcacatct attgctttgt tatacaaggc aaagcttgaa ccagcatcag
     7321 tacaacccgg ttgcaccaca gcaatccacc ggtagcacca aaagagtgca ttttaagaat
     7381 tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac aataaaacat caatatcttt
     7441 acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa agttgcagac
     7501 gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga agataaaggt
     7561 gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc tttccgcagg
     7621 actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca aaatcgccaa
     7681 aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt attacgacaa
     7741 tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg aagcagtgaa
     7801 aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct cacaaaatcg
     7861 tattccactg ctgcaaaacg gcactttcga ttttgaatgt ggttctacca ccaacaacgt
     7921 cgaacgccaa aaacaggcgg ctttctctga cactattttc gtggtcggta cgcgcctgtt
     7981 gaccaaaaag ggcggcgata tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt
     8041 cacttccggc actacctctg aagttttgct caacaaactg aatgaagagc aaaaaatgaa
     8101 tatgcgcatc atcagcgcca aagatcacgg tgactctttc cgcaccctgg aaagcggtcg
     8161 tgccgttgcc tttatgatgg atgacgctct gctggccggt gaacgtgcga aagcgaagaa
     8221 accagacaac tgggatatcg tcggcaagcc gcagtctcag gaagcctacg gttgcatgtt
     8281 gcgtaaagac gatccgcagt tcaaaaagct gatggatgac actatcgctc aggtgcagac
     8341 ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa aatccaattc cgccgaaaaa
     8401 cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga
     8461 caaggcactg aactaattac aagaaccagg ggcggaaatt ccagccctct cgattgttac
     8521 gtagcacgga cagactatac gcctgatggt cgttccccat cgggcctgaa aaccgcaata
     8581 cgctgggtaa caatcttcga gggtagcagt taacgctgct accctctttt ttctggagta
     8641 gatttatgtc tatagactgg aactggggta tttttttaca acaagccccg ttcggcaata
     8701 ccacctatct tggttgggtc tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg
     8761 cctggattat cgctttcctc gtcggttcat ttttcggcat tttacgtacc gttccaaacc
     8821 gttttctctc cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg
     8881 tgcaattctt tacctggtat ctggtgatcc cggagctgct gccggagaaa atcggcatgt
     8941 ggtttaaggc cgagctggat cccaatattc agtttttcct ttcatccatg ctctgcctgg
     9001 ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag tcgctaccgc
     9061 gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct accgcagact tatcgatacg
     9121 tactgctgcc taatgcttat cgcgttatcg tcccgccgat gacttccgag atgatgaacc
     9181 tggtgaaaaa ctccgccatc gcctcgacca tcggtctggt ggatatggcg gcgcaggcgg
     9241 gtaaattgct cgactactcg gctcacgcct gggagtcgtt taccgccatc acgcttgcgt
     9301 atgtgttgat taacgctttt atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc
     9361 tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca ttgttccttc
     9421 cctgccatat ctgctcgacg ggctggtgat caccctgaaa atcaccgtca cggcagtcgt
     9481 gattggtatt ttgtggggta ccatgctggc ggtgatgcgc ttgtccagct tcgcgcctgt
     9541 ttcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag tcatggtttt
     9601 gctgtggttt tacttgattg tgccgggatt tctgcaaaat gtgctgggat tatcgccaaa
     9661 aaatgatatt cgtttaattt cggcgatggt ggcgttttcg atgtttgaag cggcctacta
     9721 ttcagagatt atccgtgccg gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct
     9781 ggcgttgggg atgactcact ggcagtcgat gaaactgatt attctgccgc aggctttccg
     9841 cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata cctcgctggt
     9901 gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg agcgtgatgg
     9961 tacgcaggtc gagatgatcc tgtttgccgg atttgtttat ttcgttatta gccttagcgc
    10021 gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct gaaaaatgtt
    10081 tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt gaaaaaaggc
    10141 gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat caacgctgat taaaaccgtc
    10201 aacggcctcg aaccagtgca gcaaggtgaa atcaccgtcg atggtatcgt ggttaacgac
    10261 aagaaaaccg atctggcaaa gctgcgctcc cgcgtcggga tggtattcca gcatttcgag
    10321 ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa
    10381 cgcgataaag cgcccgctcg tgaaaaagca ttgaaattgc tggagcgcgt gggactttcc
    10441 gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcagcagcg cgtggcaatc
    10501 gctcgcgcat tgtgtatggc tcctattgcg atgctgttcg acgaaccgac atcggcgctg
    10561 gatccggaga tgatcaacga agtgctggac gtgatggtgg aactggcgaa cgaaggaatg
    10621 accatgatgg tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa tcgggtgatc
    10681 tttatggacg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt cgatgatccg
    10741 aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact ctggcgcaca
    10801 tacccaattg cgcgccatct cacgctttat cgtttcgccc ctcgtatctg ttacatcctg
    10861 gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact gccaattctg
    10921 ttagattgcg acccaggtca tgatgacgct atcgcaatag ttctcgcact cgcctcacca
    10981 gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga aaaaacctta
    11041 cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg atattccggt ggcaggcggc
    11101 gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg cgaaagcggt
    11161 ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag
    11221 ctgatggcga aaacgctgcg cgaaagtgcg gaacctgtca cgattgtttc taccggaccg
    11281 caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa aattgcccgt
    11341 atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc tgaatttaat
    11401 atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc ggtggtgatg
    11461 gccggtctgg atgttactca taaagcgcaa atccacgttg aagacaccga gcgtttccgc
    11521 gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt cctcgaatat
    11581 cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg caccatcgcc
    11641 tggctgttga aacccgagtt gtttaccact gttgagcgct gggttggcgt ggaaacacag
    11701 gggaaatata cccagggtat gacggttgtt gattattatt atctgactgg caataaaccg
    11761 aatgccactg taattgtcga tgttgatcgt cagggctttg ttgatttact ggctgatcgt
    11821 ctgaaatttt acgcttaaca ctcgtcattg attaagatca ccacgcgtta cctgggtggt
    11881 cttaatctat ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg
    11941 ccataggtat cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg
    12001 cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg
    12061 aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata
    12121 aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg
    12181 acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg
    12241 aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttctggct tatttcagcg
    12301 atgaacaacg caagcatacc cgtttagcag cggacttagc cgggttaaat gcggtacgct
    12361 taattaatga acccacagca gctgcgatgg cgtatggcct gcatacccaa caaaataccc
    12421 gttcgctggt gtttgatctc ggtggcggca cgtttgacgt tacggtgctt gagtacgcca
    12481 cgccggtgat tgaagttcac gcctccgctg gcgacaactt cctcggtggc gaagatttta
    12541 cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cacaaggacc acgcttaacg
    12601 agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc
    12661 caatgcacat tcgctggtag taccaggatg aaacgcggga atgcgaattt tacgagaacg
    12721 aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt
    12781 tgcgcgatgc gcgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt
    12841 cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa
    12901 gttacgatcc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac
    12961 gcagtgaaga tattgaagaa gtgatcctca ctgatatttg cccttactcg ttgggcgttg
    13021 aggttaaccg ccagggcgtt tccggcattt tctcgccgat tattgaacga aacaccactg
    13081 tgcccgtgtc gcgtgtagaa acttattcaa ccatgcaccc ggaacaggat tcaattacgg
    13141 ttaacgtcta tcagggagaa aaccacaaag ttaaaaacaa cattctggtg gaatccttcg
    13201 atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata
    13261 tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag tccagagtga
    13321 ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat
    13381 ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa gccaaactgg
    13441 aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg
    13501 attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc aggcggcggg
    13561 cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg gcgtaaatgc
    13621 tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg attttgccga
    13681 gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta atgctctgtg
    13741 gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt
    13801 catcctccgg ctcgagtttt actttcgtca acagcatccg ggtgataatg gcctctaatg
    13861 gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct tgtttcggaa
    13921 acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttctt
    13981 ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg aagaataacg
    14041 actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt tccgcccata
    14101 acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct tgcgcatcta
    14161 ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg caatgacgct
    14221 tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc
    14281 cgagatcagc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca cactggtaat
    14341 accgggcatc ttcgctttct ggttcttccg cttgccactg tttggctaca ggaatgagtt
    14401 ccaccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat
    14461 catcaggaat gaaccacggt ccttccatcg ccaaaaacgc agtgacatag tcaggatggt
    14521 aacggcagca acgttccagc gcgaagtaat attcaacagt ctggttctgt aacgcgacgg
    14581 gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag
    14641 cctccctttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct
    14701 ctgccaacaa agaagcacaa tttaatgaca gatatcgcgc ttctatagcg attgcacaca
    14761 gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt
    14821 tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac
    14881 gtagtatttc atgttcagcg gtggcatctt tttcttcatc atctgcttcc tcgacaggat
    14941 ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat
    15001 cggtttcggg atgacacaac ggtaagcgag caagataagc ctggcggata atgtctattt
    15061 gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt
    15121 aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg tggcatagaa
    15181 acggtcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta
    15241 cagggttagc gcctcgcgta gcgaagcgtt gtcttcgcac tgagaagatt gccagtagag
    15301 acgccctagt gaataggcag aaaaatagtg catccagctg tgatagtaat ggtgagctcg
    15361 tgcatagacg cgcgattgca gccataaact ctcttcttcc gtcagccagt tgttgagcac
    15421 accggcccgt gacagaaatc ccattcgaac ataatcccag gctttgattc ccccttctcc
    15481 acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc
    15541 agcaattaat gctttccact cttcagggga agagcgttgc catgtcagat ataagccagc
    15601 taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg
    15661 attatttatt ccccaggatg atgagagatc gaattctttt atcccggtat acatttttgg
    15721 cgagtcatgc gcgggtttgc aactctgatt aagtatttcc atgggcgcag aaagcgcaaa
    15781 aagaagatat tgttcttcct tattcatttt ttaataatcc cttaagtgca ttaactgact
    15841 acttttttat tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc
    15901 attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt gcagcaatta
    15961 cctgtattag ccaacccaag aaaacgatgt tgcgtgataa agccatggtg aattatgcct
    16021 ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg
    16081 cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg
    16141 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcagaacagg
    16201 aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac
    16261 ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
    16321 ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg
    16381 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
    16441 ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag tattttccct
    16501 atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag acagcccagc
    16561 ccgccatcat caacgggcat tggctgcggt tgcggctccg gctcgacggt ttcctgaccg
    16621 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
    16681 tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctggcc
    16741 tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg
    16801 agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccagc
    16861 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
    16921 ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc ttcaatcatc
    16981 atcaccacta atgggtaata atagacccga tgctgacggc accattgcaa cattaaagca
    17041 tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag caggctttcg
    17101 ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcaggt
    17161 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcggcttgcg
    17221 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
    17281 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt
    17341 tggtaaaaat cgatggtgtc attttgcacg gcaactggca gatgcagaat ggtctggaag
    17401 ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
    17461 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
    17521 aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc
    17581 tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
    17641 cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc
    17701 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc
    17761 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
    17821 aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatatcgagg
    17881 atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg catgtggggt
    17941 agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt
    18001 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata
    18061 aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
    18121 agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc
    18181 atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca
    18241 tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga
    18301 caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac
    18361 atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttct
    18421 cgatcattcg gtgaattatg cgcagcaggt catcacggtc atcaattccc catgaggaag
    18481 aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa ggtaaacagc
    18541 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt
    18601 ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc gaggctcagt
    18661 tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc aaaaatggaa
    18721 cacataaaaa tataatatat tttatctaca accataagga aatgataatg atttttacgt
    18781 caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt
    18841 gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt gactctccgg
    18901 acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac
    18961 agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag
    19021 ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg
    19081 gctggatact ggatcgtgga gaagacccca actataaaga agcggttacc tggtatcaga
    19141 tagccgcgga gagcggaatg tcttatgcgc aaaataatct tggatggatg tacagaaatg
    19201 gcaacggagt cgcacaagac tatacgctgg catttttctg gtacaaacaa gctgcattac
    19261 aaggccatag ttacgcacaa gacaatctgg ccgatcttta taaagacgga gaaggcgttg
    19321 ctcaaaacaa gacactcgcc gcattctggt atttgaaaag cgcacagcag ggtaatcggc
    19381 acgcccagtt tcaaattgca tgggattata acgctggcga aggggtggac caggactata
    19441 agcaagcgat gtactggtat ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca
    19501 acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag gctgcctttg
    19561 aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac ctggccatta
    19621 tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta
    19681 aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagctttac
    19741 tgtagaaata aaaacaaggg ccggggaatt tcattctgcc catattctct ttattgctgt
    19801 gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcata accacatttc
    19861 gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag
    19921 accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc
    19981 tggaaaattt cgcgccattc aagctgcgtt ttatcggtga tgtctgccag ctcctgccac
    20041 aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca
    20101 tagctcatgg cgaactcttc caggtcacgt ctgacagctc gcgtcagctc atcgatctcg
    20161 gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg
    20221 atatcacgtt cgccattgcg caggcgttcg ttcagtgacg cgaccagttc acggtaatat
    20281 tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt
    20341 tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt acgaataatc
    20401 cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat
    20461 gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac
    20521 cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc
    20581 attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact
    20641 gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca
    20701 ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg caaaaacgtt ctgcagccga
    20761 tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc
    20821 cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct
    20881 ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac
    20941 agaaattctt caccgagctg tataaaaaag gcctggtata taaaaagact tctgcggtca
    21001 actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct
    21061 ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg
    21121 cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta
    21181 aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta
    21241 acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt atgggttgta
    21301 cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg
    21361 aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga
    21421 cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag
    21481 aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg
    21541 cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca
    21601 aaccggttat cctggcggct gacggctctg agccagatct ttctcagcaa gccctgactg
    21661 aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa gcggccttca
    21721 acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc
    21781 tgcgcgactg gggtgtttct cgccagcgtt actggggcgc gccgattccg atggtgacgc
    21841 tggaagacgg taccgtaatg ccaaccccgg acgaccagct gccggtgatc ctgccggaag
    21901 atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta
    21961 ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct
    22021 cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag
    22081 cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc
    22141 acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg
    22201 acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg
    22261 ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg
    22321 agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca
    22381 tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt
    22441 acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg
    22501 aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg
    22561 tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag
    22621 atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc taaagtgacc gatgatatcg
    22681 gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg
    22741 cgaaagcacc gaccgatggc gagcaggatc gcgctctgat gcaggaagca ctgcaggccg
    22801 ttgtccgtat gctcaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactca
    22861 aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg
    22921 aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgtcc
    22981 cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa
    23041 aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa ctcctcaatc
    23101 tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacactgt tgttatctct
    23161 ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gacaccacgc aggttccttc
    23221 cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcggt
    23281 gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga
    23341 cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg
    23401 taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc
    23461 cggccgtgat atctacccga ttagcgccaa agtcttccgt tcattcttcg ataacccgca
    23521 aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc
    23581 tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga
    23641 agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccatgct
    23701 gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg
    23761 cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct
    23821 gttcgccagg tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc
    23881 aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga
    23941 caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt
    24001 ctcacactca ccggacttct gcatgacgac ctgctattga tcgtccgcgg taataaatta
    24061 agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg
    24121 acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgtgcg cgcaaaacag
    24181 ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta tgaaggtaac
    24241 ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaactg
    24301 acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg
    24361 gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca gcaactgcgt
    24421 ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact gttgttactg
    24481 gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg
    24541 gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagccgcag
    24601 ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt
    24661 cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa acccctggcg
    24721 gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga
    24781 tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga ttggtctgac
    24841 gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag
    24901 cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga
    24961 tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg
    25021 gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt cattgctgac
    25081 ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg
    25141 gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca
    25201 tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga
    25261 aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc
    25321 gtgtgaggat ttattgccgg aaccggtatt gacttacatt aaccaacaag gcttgtatcg
    25381 ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca tggcgaaaca
    25441 caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac cgcacgcggt
    25501 attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga cttggtttta
    25561 tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga ccgccagctc
    25621 cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat
    25681 catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag
    25741 catgctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt
    25801 atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag ccatcagggc
    25861 gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat gtggcatttt
    25921 cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc
    25981 gtcctcaata gccgtgccat cggggtcgag aacgcatgac ttttctgttt ttttacgggt
    26041 aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc cagacttttt
    26101 ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg
    26161 gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct
    26221 ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt
    26281 gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga
    26341 catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga tcatctgtac
    26401 gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc
    26461 agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga
    26521 tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga
    26581 aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga
    26641 ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga
    26701 gctgattgaa attccggccg gcaaacgcgg caagaatgcg gacatcaagc gcatactcga
    26761 caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat
    26821 tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga
    26881 tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc
    26941 ggcggctgag cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt
    27001 gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg
    27061 tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg
    27121 cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat
    27181 tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac
    27241 cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg
    27301 cggcattatc tacgaccgta acggtatccc tctggccctc aaccgcacta tctaccagat
    27361 agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt
    27421 ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg
    27481 tttcacctct attccggtga aaactaacct aaccgaagta caagtagctc gctttgccgt
    27541 caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta
    27601 cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga
    27661 acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata ttggtaagct
    27721 gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga
    27781 agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca
    27841 cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg
    27901 tagccgcgca gctgtggttg ttaccgaacc gcgtacaggt ggggtgctgg cgctggtttc
    27961 cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc
    28021 cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc
    28081 cgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg tgatcacgcg
    28141 caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa aacgttatcg
    28201 tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc
    28261 ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat
    28321 gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa
    28381 catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac
    28441 cattccggtt ggtatcggcc agggttactg gacagcgacc ccaatccaga tgagtaaggc
    28501 actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga tgagcaccgc
    28561 cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg gcgatattca
    28621 ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaatgg
    28681 tacggcgaat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ctggtaccgc
    28741 tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt
    28801 acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc
    28861 catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc gccagatcct
    28921 cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc
    28981 cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa
    29041 gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta cagcgccctg
    29101 gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc
    29161 gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc
    29221 tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga tgctttcgga
    29281 gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg
    29341 gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc
    29401 ccgccatcgt tgaagaatac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg
    29461 gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta
    29521 ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtagcggcg
    29581 ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg
    29641 ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt
    29701 gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt
    29761 gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga agagctggga
    29821 ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg
    29881 atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt aatgctgata
    29941 ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg
    30001 gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc
    30061 gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg
    30121 cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac
    30181 aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc
    30241 catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca
    30301 ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca
    30361 ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg cctctggcga
    30421 agctttcgat ccgacaaagc tgacggcggc ccatccaacg cttccgatcc ccagctacgc
    30481 cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc
    30541 ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc ttaacacgtc
    30601 aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg
    30661 tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg cacctcccga
    30721 tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca ttcttccggt
    30781 cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt
    30841 cctcggcgct ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc
    30901 gccacagccc gttgttacag cttcgtcgac aacgcctgca acctcgcctg caatggtgac
    30961 accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgtaag
    31021 cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg
    31081 tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg ccagcaaagc
    31141 cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac
    31201 taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca
    31261 aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt
    31321 taattccatc acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat
    31381 gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga
    31441 tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat
    31501 cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga
    31561 tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg
    31621 taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca ccggtaaccc
    31681 ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca
    31741 gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca tggccgattt
    31801 tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta acgcactggg
    31861 cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc
    31921 cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga atgaatactc
    31981 gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct
    32041 gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg
    32101 ttacaatctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cggtgatggg
    32161 tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg
    32221 tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg
    32281 gtttagtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc
    32341 gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc
    32401 gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat
    32461 cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat
    32521 cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa
    32581 gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct tctggcggga
    32641 gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa cgaactgctt
    32701 gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga gctggttgat
    32761 caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac ggtaaaacca
    32821 agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca tatcgagcag
    32881 gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt tctgtaactc
    32941 gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt gatatacttt
    33001 tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata aaattcttgt
    33061 ccgccagctc ggtcttcagc cttacgagcc aatctcccgg gctatgcatg aattcaccga
    33121 tacccgcgat aatagtaccc ttgatgaaat ctggctggtc gagcactatc cggtattcac
    33181 ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc cggtgatcca
    33241 gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga tgtatgtgtt
    33301 gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac
    33361 agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg acgcgcctgg
    33421 tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac gcggttgttc
    33481 attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac gtattaatcc
    33541 ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg aagcgacgac
    33601 taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca atccggactt
    33661 cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg gtcattactc
    33721 ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt atcacttgca
    33781 gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta ttgaaatagt
    33841 tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg taatttttta
    33901 aatattattt ttccatgaat aaaattggag taagtgcgtg gatagtaata atcaaattga
    33961 accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa ccttacgaaa
    34021 tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag ggatcgttaa
    34081 tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc agaaactgcg
    34141 cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc ctaccgcatt
    34201 tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg gcgcgctgga
    34261 tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc cctcggtcgg
    34321 agccctggtc cttcctgtca tctatcgggc gattaaaact cactatccgc agcttttact
    34381 gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat
    34441 catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa
    34501 tatggtgtta atttgccgca agggaaatcc actactctct ttagaagatg acagagagac
    34561 tatcgacaac gctgcgcatg tacttctgtt accggaaggg caaaatttca gcggtctgcg
    34621 gcagagagtt caagagatgt ttccggaccg gcaaatcaat ttcaccagct acaacatttt
    34681 gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca gccgttttta
    34741 taacctgttt agtcgttgct ggccgctgga aaaactgcct tttccgtcct taaatgagga
    34801 gcaaatagat ttctctatcc actacaacaa attcatcctg cgtgatccca tcctgcacgg
    34861 agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca gatcacagcg
    34921 cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc gctttggctg
    34981 ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag ttgataatta
    35041 caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac acgcacgcta
    35101 tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat aagatggccc
    35161 ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag ccggaatgga
    35221 tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc gcaatgcgca
    35281 aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg gaatgcttca
    35341 accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt tgtccgttct
    35401 gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg aaactggcgc
    35461 agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac cgtgatgacc
    35521 tgcgcgatgg cggtgcccaa cactttgcgg attgtattac tgccattcgg gaaaaaagcc
    35581 cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg tcgaatggat cgtgctctgg
    35641 atattctgac tgcaacgccc ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta
    35701 tttaccggca ggtacggcct ggcgcagatt acaactggtc gctgaagctg ctggaacgct
    35761 ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtgggt ctgggtgaaa
    35821 ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa
    35881 cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc
    35941 cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc catgctgcat
    36001 gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaagga atggaagtta
    36061 agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt tgaagcgggt
    36121 tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact ctttatgaga
    36181 gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat cgtcatcatt
    36241 catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg tacgtaactt
    36301 cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg taatactaat
    36361 ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt aaaaaccaga
    36421 gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag gtgaatcaag
    36481 cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt gtttaagatg
    36541 ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat aatgagtgca
    36601 ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg gctttgcccg
    36661 atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc cagcgcacga
    36721 gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca ggcggcagga
    36781 agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg ccagctctgg aaagcgtaag
    36841 tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg agctatttca
    36901 ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag atgcagtttg
    36961 gcataacggg cgacgatgtt tcctgcctga agtgccacca gcatattcca tgcgcgcccc
    37021 ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca tgttacgttt actttctcgc
    37081 cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga tagatctgca
    37141 tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa acagcgatac gtcgttttcc
    37201 gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac agatgtaaca
    37261 gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc agagtcgtaa aattcgccaa
    37321 cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg attttgcagt
    37381 gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtag cgagatggct
    37441 gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc tggcagcaaa
    37501 cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga tgacgaacat
    37561 tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa caaccttctc
    37621 aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct gggcattact
    37681 gaacgttttc gtcaaccttc tggggtcttt tgccatgacc gcactggcat tctggctgtt
    37741 ttcggcctca accgtacact aaagaccaaa aaaaacccgc tgattaagcg ggttttgaat
    37801 tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt tggcaccgtt
    37861 agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat tagtctggat
    37921 tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc
    37981 tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa ttacctttac
    38041 atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca ccaattgtgg tacgctttgt
    38101 ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt gcttatttta
    38161 gttattgatt ttaaagaagt tactaaaact tcatttgtct caaaactgaa agcgcatcca
    38221 ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct gaaaagtcac
    38281 tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt aattccagtt
    38341 atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt catccgccag
    38401 cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt tccacgagtc
    38461 cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac gcgacaggcc
    38521 aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa
    38581 acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct gttgccaggt
    38641 ttgtgcaata ttttctctaa acgttgtcat cctctcatct gcgttagcaa aaactttacc
    38701 aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac tcacgttcat
    38761 ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct acaattaaca
    38821 taaccttaat agacccaaca taaagaataa tctgaatagc tgctctattt atttccgctt
    38881 gttcaagtta aatatttccg cgcatcgatt aaagatgatg cgtcgttttc tgcgatggga
    38941 atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa atgcaatcaa ggaaaaatta
    39001 ttttattttt ttaacgagat acaacaatca tcttaacgaa gtatataata tatatcgtca
    39061 acccatgctc atattagatt attttttgag cgcacagccg cagctaacac cattgcaatt
    39121 aacaaatttg catcaatcca ccatcaattt acacacatta ttatgtgata attgccaacc
    39181 gctaaatatg cgttttgtta tctatgtata aaaacagcaa cttcaatgtc ttaatggcag
    39241 tttttcttga ttttaatcag cattcatcgc caatttattg ggcatatttt ttccttaagc
    39301 tttaggaagt ttttattatt tactttgggc ctggagacag gaaaaattat gctgacattc
    39361 attgaactcc ttattggggt tgtggttatt gtgggtgtgg ctcgctacat cattaaaggg
    39421 tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat cagtgccatt
    39481 atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc cacggatatc
    39541 gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat gatgattatg
    39601 atgctgtgtg gctttgccgc ttacatgacc catatcggcg cgaatgatat ggtggtcaag
    39661 ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat tgccgcctat
    39721 tttgttgctt gtctgatgtc actggccgtc tcttccgcaa ccggtctggg ggttttgctg
    39781 atggcaaccc tgtttccggt gatggtaaac gttggtatca gtcgtggcgc agcagctgcc
    39841 atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt ggtgctggcg
    39901 gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct gcctatctca
    39961 attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta tctggataaa
    40021 aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac tgcccccgcg
    40081 ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga cggtaaatgg
    40141 ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc ctccattctg
    40201 gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt ggcttatcgc
    40261 gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg ggtattcgct
    40321 caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac ctcgtttggt
    40381 tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc ggcagtcacg
    40441 accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa actggcgcac
    40501 tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc aaacctgggt
    40561 cgtaccctat cacccgtttc tggcgtagtc gttgcggttg ccgggatggc gaaaatctca
    40621 ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg ttgggctagt gattgttatc
    40681 gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa gtaaatagta
    40741 atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat atgcgttgtg
    40801 gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg gcgcaatatt
    40861 caagataacg cctggcagtg gtgcggctga tggtcagtgc ctgcgccacc gtttctgccg
    40921 tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc aaggggtcaa
    40981 tgccggtcgg cagttcgtct ttgggttcac cgcgagcata ggcattaaac atctcatcaa
    41041 tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg cggaaacggg
    41101 ttagcgtttg ccccagtgta ttgattcact tgaagtacga aaaaaccggg agaacattgg
    41161 attattcggg atctgatggg attagatttg gtggggcttg caagcctgta gtacaaattt
    41221 taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt gcttttgttt cattgaaaaa
    41281 acgaaaaaca aaaacactat aaatcatttc aatcatagct tcaaaaaacg ttcaaaaccg
    41341 ataacaacca ggctgtcacc aaatgactca tatcacaaat cagctcatgc cgtttaggta
    41401 tgttacatgt gtgattatgt gaggtgaagt atgttttagc tggctcatgg ttgttatacg
    41461 gcttttttcg catcttgtgg ttccaacaaa ggagctacag gctcttcctg tttatgaaca
    41521 ccatacattg acaaaatctc ttggcgagtt tctggaggta atttatgcag cgccaaagct
    41581 gtttgtagtg tttggcgatc aatacccagc gttgccaata gggtgtcaac ggagatggag
    41641 gggcgttctt gcattaatcc ggaaagccca tatttagcca tgtttagctc tatctctcgg
    41701 acttcatttt ctcctagttt attgattaac agggagaaca ttttcaatgt atcagcattc
    41761 atttcttcga tagcaatttt ttgcatagcc ggtattgccg ggatatattc aaccccaggc
    41821 ccgcgacgcc cttcttgtga acgcttcggc catccttctt tctttgctcg tttatgcacc
    41881 ccctgtattg tcccaggcat tccatccagt tcagtaattt gtttgggtgt catccactgg
    41941 tttatctcat ttgcgctttt cataaactaa gtttaccaga tttatctata taaaaatcaa
    42001 tggattacaa aataaaccac attaactgat ataaaccggt ttggtttatt tggtaatata
    42061 taaaacagat aatcaagacg aattatttgt tttggtaacc ctttatcata cgcaatatgg
    42121 ttggtgtgcg aaagatgagt aacgattggc atcgggccga cgtgattgcg gccctcaaaa
    42181 aaacaggaac ccctctggca aaattgtcca gacgttcagg ttattcaacc gggacgcttg
    42241 caaatgcatt agtcaggcca tggcctaaag gtgaacgcat tattgctgac gccttaaaca
    42301 tgaaacctga ggaaatttgg cctagtagat atctaaggga tcacaatgag agagtggata
    42361 tcagcaattg aatgcatggg tttatctggt ttacctggtt cattaatggg ggttcacaaa
    42421 agagctcaga gagaaggatg gctcaagaga agaagagaag gaattagagg ggttgcttac
    42481 gaataccata tatcttctct tcctaacatt gctcgagctg aagttttgct ccaacaagga
    42541 aaatttaaaa ccagtcaggg atgttttgaa atcgcccgcc ccacgctgga agctcatgat
    42601 tatgatcgtg aggcactatg gagcaaatgg gataaagcca gcgattccca gcgcagcctg
    42661 gctgaaaaat ggttgccctc aattcaggcc acagacgaaa tgctgaacca ggggatttca
    42721 acgaaaacgg cttttgcgac cgttgcaggg cattaccagg tcagcgcatc cactttgcgg
    42781 gacaagtatt accaggtaca gaagtttgcg aagcctgact gggcggctgc acttgttgat
    42841 ggacgtggag catcccgtcg caatgttcac aaaagtgaat ttgacgagga tgcctggcag
    42901 tttctgattg cagattatct gcgaccggaa aaacccgcct tccgcaaatg ttatgagcgt
    42961 ctggaactgg cagcccgcga gcatggctgg agtattccct cccgtgccac ggcctttcgc
    43021 cggattcagc aactggacga ggcaatggtt gttgcctgtc gtgaaggtga acatgcactg
    43081 atgcatctga taccggcaca gcagcgaact gtggaacacc tggacgccat gcagtggatc
    43141 aacggcgacg gttatctgca taacgtcttt gtacgctggt ttaacggtga tgtgatccgt
    43201 ccgaaaacat ggttctggca ggatgtgaaa acccgaaaaa ttctgggctg gcgctgcgat
    43261 gtgagcgaga acattgattc aattcgcctc tcgttcatgg atgttgtgac tcgctacagt
    43321 atcccggagg attttcacat caccattgat aacacccgtg gtgctgcgaa taaatggctg
    43381 acgggaggcg cgcccaatcg ctaccgcttt aaggtaaaag aggacgatcc aaaaggactg
    43441 tttttactga tgggtgcgaa aatgcactgg acaagcgttg
//
LOCUS       DAJVPT010000022        42998 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVPT010000022 DAJVPT010000000
VERSION     DAJVPT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            42..1475
                     /gene="cscA"
                     /locus_tag="OQM47_001181"
     CDS             42..1475
                     /gene="cscA"
                     /locus_tag="OQM47_001181"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1479415.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1483..2541)
                     /locus_tag="OQM47_001182"
     CDS             complement(1483..2541)
                     /locus_tag="OQM47_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1479416.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2709..2927
                     /locus_tag="OQM47_001183"
                     /pseudo
     CDS             2709..2927
                     /locus_tag="OQM47_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2945..4273
                     /gene="dsdA"
                     /locus_tag="OQM47_001184"
     CDS             2945..4273
                     /gene="dsdA"
                     /locus_tag="OQM47_001184"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1479417.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4381..5919)
                     /gene="emrY"
                     /locus_tag="OQM47_001185"
     CDS             complement(4381..5919)
                     /gene="emrY"
                     /locus_tag="OQM47_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1479418.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5919..7082)
                     /gene="emrK"
                     /locus_tag="OQM47_001186"
     CDS             complement(5919..7082)
                     /gene="emrK"
                     /locus_tag="OQM47_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1479419.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7498..8112
                     /gene="evgA"
                     /locus_tag="OQM47_001187"
     CDS             7498..8112
                     /gene="evgA"
                     /locus_tag="OQM47_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1479420.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8117..11710
                     /gene="evgS"
                     /locus_tag="OQM47_001188"
     CDS             8117..11710
                     /gene="evgS"
                     /locus_tag="OQM47_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1479421.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11766..12911)
                     /gene="yfdE"
                     /locus_tag="OQM47_001189"
     CDS             complement(11766..12911)
                     /gene="yfdE"
                     /locus_tag="OQM47_001189"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1479422.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12985..13929)
                     /gene="yfdV"
                     /locus_tag="OQM47_001190"
     CDS             complement(12985..13929)
                     /gene="yfdV"
                     /locus_tag="OQM47_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1479423.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13999..15693)
                     /gene="oxc"
                     /locus_tag="OQM47_001191"
     CDS             complement(13999..15693)
                     /gene="oxc"
                     /locus_tag="OQM47_001191"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1479424.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15747..16997)
                     /gene="frc"
                     /locus_tag="OQM47_001192"
     CDS             complement(15747..16997)
                     /gene="frc"
                     /locus_tag="OQM47_001192"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1479425.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17510..18133)
                     /locus_tag="OQM47_001193"
     CDS             complement(17510..18133)
                     /locus_tag="OQM47_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1479426.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18430..18705
                     /locus_tag="OQM47_001194"
     CDS             18430..18705
                     /locus_tag="OQM47_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479427.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18782..19024)
                     /locus_tag="OQM47_001195"
     CDS             complement(18782..19024)
                     /locus_tag="OQM47_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1479428.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19377..20297
                     /gene="lpxP"
                     /locus_tag="OQM47_001196"
     CDS             19377..20297
                     /gene="lpxP"
                     /locus_tag="OQM47_001196"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1479429.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20652..20723
                     /gene="ypdK"
                     /locus_tag="OQM47_001197"
     CDS             20652..20723
                     /gene="ypdK"
                     /locus_tag="OQM47_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1479430.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20788..22026)
                     /gene="alaC"
                     /locus_tag="OQM47_001198"
     CDS             complement(20788..22026)
                     /gene="alaC"
                     /locus_tag="OQM47_001198"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1479431.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22403..23889
                     /locus_tag="OQM47_001199"
                     /pseudo
     CDS             22403..23889
                     /locus_tag="OQM47_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23904..24638
                     /gene="ypdB"
                     /locus_tag="OQM47_001200"
     CDS             23904..24638
                     /gene="ypdB"
                     /locus_tag="OQM47_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1479432.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24651..25508
                     /locus_tag="OQM47_001201"
                     /pseudo
     CDS             24651..25508
                     /locus_tag="OQM47_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25511..28006)
                     /gene="ptsP"
                     /locus_tag="OQM47_001202"
     CDS             complement(25511..28006)
                     /gene="ptsP"
                     /locus_tag="OQM47_001202"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1479433.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28031..29068)
                     /gene="ypdE"
                     /locus_tag="OQM47_001203"
     CDS             complement(28031..29068)
                     /gene="ypdE"
                     /locus_tag="OQM47_001203"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1479434.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29068..30151)
                     /gene="ypdF"
                     /locus_tag="OQM47_001204"
                     /pseudo
     CDS             complement(29068..30151)
                     /gene="ypdF"
                     /locus_tag="OQM47_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30166..31413)
                     /locus_tag="OQM47_001205"
     CDS             complement(30166..31413)
                     /locus_tag="OQM47_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1479435.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31435..31761)
                     /locus_tag="OQM47_001206"
     CDS             complement(31435..31761)
                     /locus_tag="OQM47_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1479436.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31980..32945)
                     /gene="glk"
                     /locus_tag="OQM47_001207"
     CDS             complement(31980..32945)
                     /gene="glk"
                     /locus_tag="OQM47_001207"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1479437.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33149..34405
                     /locus_tag="OQM47_001208"
     CDS             33149..34405
                     /locus_tag="OQM47_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1479438.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34520..34846
                     /locus_tag="OQM47_001209"
     CDS             34520..34846
                     /locus_tag="OQM47_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1479439.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34987..36225)
                     /locus_tag="OQM47_001210"
     CDS             complement(34987..36225)
                     /locus_tag="OQM47_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1479440.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36561..37763
                     /gene="nupC"
                     /locus_tag="OQM47_001211"
     CDS             36561..37763
                     /gene="nupC"
                     /locus_tag="OQM47_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1479441.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37813..39925)
                     /locus_tag="OQM47_001212"
                     /pseudo
     CDS             complement(37813..39925)
                     /locus_tag="OQM47_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40133..40208)
                     /locus_tag="OQM47_001213"
     tRNA            complement(40133..40208)
                     /locus_tag="OQM47_001213"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40173..40175),aa:Ala,seq:ggc)
     gene            40429..40788
                     /locus_tag="OQM47_001214"
     CDS             40429..40788
                     /locus_tag="OQM47_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1479442.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40790..41182
                     /locus_tag="OQM47_001215"
     CDS             40790..41182
                     /locus_tag="OQM47_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1479443.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41234..42649)
                     /gene="gltX"
                     /locus_tag="OQM47_001216"
     CDS             complement(41234..42649)
                     /gene="gltX"
                     /locus_tag="OQM47_001216"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1479444.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42908..42983
                     /locus_tag="OQM47_001217"
     tRNA            42908..42983
                     /locus_tag="OQM47_001217"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42941..42943,aa:Val,seq:tac)
BASE COUNT        11441 a        10716 c         9978 g        10863 t
ORIGIN      
        1 cgttaataat tcaccaaatt tgcttaacca ggatgattaa aatgacgcaa tctcgattgc
       61 atgcggcgca aaacgcacta gcaaaacttc atgagcaccg gggtaacact ttctatcccc
      121 attttcacct cgcgcctcct gccggatgga tgaacgatcc aaacggcctg atctggttta
      181 acgatcgtta tcacgcgttt tatcaacatc acccgatgag cgaacactgg ggaccaatgc
      241 actggggaca tgccaccagt gacgatatga tccactggca gcatgagcct attgcgctag
      301 cgctaggaga cgataatgac aaagacgggt gtttttcagg tagtgctgtc gatgacaatg
      361 gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt aatgacgatg
      421 caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc gagaaacagg
      481 gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa gtgtggcgtg
      541 aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg gggcagatcc
      601 tgctttatcg cggcagttca ttgcgtgaat gggccttcga tcgcgtactg gcccacgctg
      661 atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc gatcagcatt
      721 atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga aatcgctttc
      781 aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa tccgggcatt
      841 ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta gcgaaggatg
      901 gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc tcaaaacgtg
      961 aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat ggcaaacttc
     1021 tacaacgccc ggttcacgaa gctgagtcgt tacgccagca gcatcaatct gtctctcccc
     1081 gcagaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag attcagttgc
     1141 agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc actggaatgc
     1201 ggctgtatat tgataaccaa tctgagcgac ttattttgtg gcggtattac ccacacgaga
     1261 atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc ctaaggatat
     1321 ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg atgagtagtc
     1381 gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac ggagtggctg
     1441 tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg aacaacggat
     1501 caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa taatatgcaa
     1561 tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg gcggtaaaaa
     1621 cagatgcccg acgccaacca gattatcaaa gcccattaca gcgacatcct gcgggatacg
     1681 tacccccttc gccaaaagaa cctgataagc cacaaaggct gcgcgatcgt taccacatat
     1741 cagaacatca aaatctggtt tgcccgattt gaagtgagca ttgagtaaac ttgcgagatc
     1801 ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca gatctcgtcc
     1861 agcatcacgc caagcctgct caaatccctg ccgacgatac cctgttgcca acgcactttc
     1921 cggtagccag aagcataatg gttgacgata gcccgccgcg agcaaatgct gtgttgattc
     1981 atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat ccgccacaca
     2041 gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat gtcgcagccc
     2101 cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg ccgcgcgggc
     2161 agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact cgctggcggt
     2221 ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt cctgcgccag
     2281 cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg aaagatcagg
     2341 aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag gacgcacaga
     2401 ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca ggcgtgcgac
     2461 atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt agcagaaaca
     2521 gacgtcactg gcgagatcca acgccctatc acctgacaca gcaatacaat aaaaaataac
     2581 aataattccc ggacaattgt ccccaattcc gcctctgttc tcgccaacga gtaaccatat
     2641 aggcaagtaa gtaaaacatg ccaactggca tcgccaacct ggagaagaaa atataaatat
     2701 ggatcattgt gctgccactg tatcccgaca tcagcccgga aattattgcg attgctatcg
     2761 gttcaggtgc aattggctgc acgatcgtta cggactcgct tttctggctg gtgaagcaat
     2821 attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca tttatcgctt
     2881 cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg caaagagacg
     2941 tactatggaa aacgctaaaa tgaactcgct catcgcccag tatccgttgg taaaggatct
     3001 ggttgctctt aaagaaacca cctggtttaa tcctagcacg acctcattgg ctgaaggttt
     3061 accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct tatcccgttt
     3121 tgcaccctat ctggcaaaag catttgctga aacagctgcc actgggggga ttattgaatc
     3181 agaactggtt gccattccgg ctatgcaaaa acggctggaa aaggaatatc aacaaccgat
     3241 cagcgggcaa ctcctactga aaaaagatag ccatttgccc atttccggct ctataaaagc
     3301 acgcggcggg atttatgaag tcctggcaca tgcagaaaaa ctggctctgg aagcggggtt
     3361 gctgacgctt gatgatgact acagcaaact gctttctccg gagtttaaac agttctttag
     3421 ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg gcattatgag
     3481 cgcccgcatt ggctttaagg tgacagttca tatgtctgct gatgcccggg catggaaaaa
     3541 agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt atggtgttgc
     3601 cgtcgaggaa ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta ttgatgacga
     3661 aaattcccgc acgttgttcc ttgggtattc cgttgctggc cagcgtctta aagcgcaatt
     3721 tgcccagcaa ggccgtatcg tcgatgctga taaccctctg tttgtctatc tgccgtgtgg
     3781 tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg gcgatcatgt
     3841 tcactgcttt tttgccgaac caacgcactc cccttgtatg ttgttaggcg tccatacagg
     3901 attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg cagcggatgg
     3961 ccttgcagtt ggtcgcgcat catgctttgt cgggcgggca atggagcgtc tgctggatgg
     4021 cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg cgcaggaaga
     4081 aggtattcgt cttgaacctt cagcactggc gggtatggcc ggacctcagc gcgtgtgtgc
     4141 atcagtaagt taccaacaga tgcacggttt cagcgcagaa caattgcgta atgccactca
     4201 tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatgaatc aatatctggc
     4261 aaaaggccgt taataacgtt tcaacgcaac atcgcaatcc tttccctggg tgagcgatgc
     4321 tgccgatggc gcagacttaa gatccccggt cttacccgct ataaccccct ccttttatca
     4381 tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa ccgtaagtaa
     4441 gataaaagca atagccgcca tacggaaaat ctcatttgca gaaatagaaa gtgactgctg
     4501 ggttatttca ttattaattt cattaagaac tcctgaaagc gaaccatagt atttatccat
     4561 aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg tcaactgact
     4621 atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac caactgatcc
     4681 tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat tatctggcaa
     4741 gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga atccctggaa
     4801 aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca cagaacgcca
     4861 gtaatagcaa accgcataca tcaaaaaact gaatgtcact aacacccgca tgtctatttt
     4921 attgccataa cgtcctatca aaggtgaaat caatagtggc atgatgccga tgggcgcata
     4981 agcaagcccg gcccatatcg cattataccc catcgttttc tggagtaact gcggcataag
     5041 gacgatcgct ccagagtaaa ataaatacgc gcaggtgata ctcacaatac caatggtgaa
     5101 attacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg attcccaaat
     5161 gactaaagag atcagagaaa taactgatac tactgttaat attattattg tactcgagtt
     5221 gaaccaatcc agatcgcgcc ctttatcaag cataatttgc aagccaccaa caccgagcac
     5281 taacagggtc agtcttggta gattcatttt taccggtgaa gtttcagttt ctcttccttt
     5341 aagtaaggtt aagcataatg tcaggacgat aatccccata gggacattga ttaaaaatat
     5401 ccaaccccag ctaaagttat cgcaaatata gccgcccaat atcggcccac atatcggagc
     5461 gataatcacg gtcattgacc ataatgccag agcaaatgtt cttttttctg gtggataatt
     5521 ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc cctgaacgac
     5581 tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg aagacagcga
     5641 aaaaaaagtg actgaaagta aaaataacct taattcgcct attctttgtg ccagcctgcc
     5701 ggtaacaggg atcgcaatgg catttgctac gccaaacgag gtgataaccc aggtgccttc
     5761 gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag aaatagtgga
     5821 atccaacatt tgcataaacg tcgctaatga caatgcaata gtaacgcacc ataacgtccc
     5881 accggttaat ggtgccggag ttgatttagt gattgccatt aaagttgtcc attatgcgaa
     5941 ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc cttactggta
     6001 taagcaggca tggaggtcac ggttgaagcc agatcaggca tctcagcaat gtcttcgtcc
     6061 ttcgtatcaa tagttgcagt catcgataaa ccaatgcgca aggggtgttc catgagttct
     6121 tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca gttccctgtc
     6181 gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat ccctgtcacc
     6241 cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac tgattgacca
     6301 atccgtacat ccgtgagttg tgtttcttta aagttggcat taacccacat ttgacgtgcc
     6361 ggtacgacag ccattaacga ttgtccggaa ctcactgttt cgccgacctg aacacttcgc
     6421 tgggcaatat agccagtaac cggactcctt atatccgtac gtttaagcac caaccaggct
     6481 tctttggttg catccgccgc ttcaactact tgtggctgac ggtttaatgg tgtgttcatt
     6541 actaaagctt tattcgcttt ataagcctgg atagcggcat tcagtgccgc tttgctactt
     6601 attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg cttcgctaaa
     6661 ggcacacgac ggttataatc ttctaaagat tgttgatact gaatacgtgc tgaagcgact
     6721 tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac aatatttgcc
     6781 agattatttt tagctttatt gagtgcgata gtggcatcag ttttatccag tgaaactaaa
     6841 atgtcaccct gtcgaacgta gttcgtatct ttatgattaa ctacagtgac actacctgag
     6901 acttgtgcag aaattggatc tgcattcccc gtgacatagg cgtcatctgt actaatcatg
     6961 tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa taaaactatc
     7021 accaataaag aaaagtattt ttttctgtta gactgttttt tatttgaatt aatctgttcc
     7081 actattatct ctcatttcgc atagatgaaa tttatgaatt gaatgaaaag agtattcatt
     7141 cttcatatca aacacagtgc atgtgttgca cattatccca ggaacgttgg aggcctaatt
     7201 taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa tacaattctt
     7261 acgcctgtag gattagtgag aagacttata gtgctaactt gaaactataa atcatcggta
     7321 taatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg gtatacttgt
     7381 cgaattatct taaaggaaac tcagattttc ttatttttat tgagaaaatg agatgacgcc
     7441 ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga ataatctatg
     7501 aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa tttattgatc
     7561 aaaaacgata ttgaaatctt agcggagtta actgaaggcg gaagcgccgt tcagcgggtg
     7621 gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt taacggtatc
     7681 caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat cgtctccgct
     7741 aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg tttcgtgagt
     7801 aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc
     7861 tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc
     7921 gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat
     7981 aatgacattg cggaaaaaat gttcatcagc aacaaaactg tcagcacgta taaaagtcgc
     8041 ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc gcaacgtaac
     8101 aaaatcggct aatcacatga agtttttacc ctattttttt cttctctgtt gtggtctttg
     8161 gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca gtagtaacaa
     8221 ccgtgtcaca cttgatccag tacgtctgag caacaaggaa ttacgttggt tagcgagcaa
     8281 aaaaaatctt gtgattgcag tacacaagtc ccaaacggct accttgttgc ataccgattc
     8341 gcagcaacag gttcgtggta ttaatgctga ttatttaaat cttttaaaaa gagcgttaaa
     8401 tatcaaatta acactccggg aatacgcaga tcatcaaaaa gcaatggacg cgcttgcaga
     8461 aggtgaagtc gatatagtgt tatcacattt agttacttcg ccgcctctta ataatgacat
     8521 tgctgcaacc aaaccattga taattacctt tccggcgctg gtaaccaccc ttcacgactc
     8581 aatgcgaccg cttacctcac caaaaccagt aaatattgct cgggtagcaa attacccccc
     8641 agacgaggta attcatcaat catttccaaa agcaacaatt atctctttta caaatttata
     8701 tcaggcatta gcatccgtct cagctgggca caatgattac tttattggta gtaacatcat
     8761 taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga aatattataa
     8821 ctcgccgcgt caatataatt ttttcttgac cagaaaagaa tctgtcattc ttaatgaagt
     8881 actcaataga tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat cacaaaattg
     8941 gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg aacatgaaaa
     9001 acagtggatt aagcagcatc ccaatttaaa ggtgctggaa aatccttact cgccccccta
     9061 ttctatgacg gatgaaaatg gctcggttcg gggcgttatg ggggacattc ttaatattat
     9121 taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata tccatgctgg
     9181 aacacaactt agccccggag gatgggatat aatacctggc gctatttata gtgaagatcg
     9241 agaaaataat gttttatttg ctgaagcctt cataacaacg ccttacgttt ttgtcatgca
     9301 aaaagcgcct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca ttccatatta
     9361 ttatgagctg cattcgcaat taaaagagat gtatccggag gttgaatgga tacaggtcga
     9421 taatgccagc gctgcatttc acaagattaa ggaaggtgaa cttgatgctc tggtcgcgac
     9481 acagctaaat tcgcgttaca tgatcgatca ttactatcct aatgaacttt atcattttct
     9541 tattcctggc gttccgaatg catcgctttc gttcgctttt cctcgcggag aaccggaact
     9601 taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc tgcgcctgac
     9661 ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctat atagcgagca
     9721 attttatatt gttacgacat tatccgtttt attagttggc agtagccttt tatggggatt
     9781 ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag aaaaccaaat
     9841 atcattccga aaagcactct cggattcctt accgaatcca acttatgttg taaactggca
     9901 aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg attactacaa
     9961 aaatgcaatg ttaccattag aaaacagtga ttcacccttt aaagatgttt tttctaatgc
    10021 gcatgaagtc acagcagaaa cgaaagaaaa tcgaacaata tacacacagg tatttgaaat
    10081 tgataatggc atcgagaaaa gatgcattaa tcactggcat acattatgca atcttcctgc
    10141 aagtgacaat gcagtatata tttgtggttg gcaagatatt actgaaacgc gtgatctaat
    10201 taatgcactc gaggtagaaa aaaataaagc gataaaggct accgtagcaa aaagtcagtt
    10261 tctggcaacg atgagtcacg aaataagaac accaataagc tctattatgg gcttcctgga
    10321 acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt cacttgccta
    10381 cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg acaaaattga
    10441 atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag tccagaacac
    10501 ttgtcactct ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt gcagcagtac
    10561 atttcctgat cattacctgg ttaagatcga ccctcaggcg tttaagcagg tcttatcaaa
    10621 tttactgagt aatgctctca aatttaccac cgagggggca gtaaaaatta cgacctccct
    10681 gggtcacatt gatgacaacc acgctgttat caaaatgacg attatggatt ctggaagtgg
    10741 attatcgcag gaagaacaac aacaactgtt taagcgctac agccaaacaa gtgcaggtcg
    10801 tcagcaaaca ggttctggtt taggcttaat gatctgcaaa gaattaatta aaaatatgca
    10861 gggcgattta tcattagaaa gtcatccagg cataggaaca acatttacga tcacaatccc
    10921 ggtagaaatt agccagcaag tggcgactgt cgaggcaaaa gcagaacaac ccatcacact
    10981 acctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc tattactcaa
    11041 acgccagcta aatctattag gatatgatgt tgatgaagcc actgatggtg tgcaagcgct
    11101 acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata tgccgaatat
    11161 ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac ccatctgggg
    11221 gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg gcatgaactt
    11281 atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc agttacacca
    11341 ggttgcgcat attgcacctc agtatcgcca ccttgatatc gaagccctga aaaataatac
    11401 ggcgaatgat ctacaactga tgcaggagat tctcatgact ttccagcatg aaacgcataa
    11461 agatctaccc gctgcgtttc atgcactaga agctggcgat aacagaactt tccatcagtg
    11521 tattcatcgc atccacggtg cggctaacat cctgaatttg caaaagttga ttaatattag
    11581 ccatcagtta gaaataacac ctgcttcaga tgacagtaag cctgaaattc ttcagctgct
    11641 gaactctgtt aaagaacaca ttgcagagct ggaccaggag attgctgttt tctgtcagaa
    11701 aaatgactaa attgcggctc ccacaatgtt ctaatgtggg agctatttac cagcacatct
    11761 tacatttatg atgagaactc ctggcgaatt tgttccccat gctggtcgag cgttgccgct
    11821 cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc cggcatcatt
    11881 attcctccgg cttcaatcaa catatttctc gcctgagttt gtggcaaatt aatggcctca
    11941 gccacactta ataacggcgc gacgggcaca ccaacttcat gtattttggc taaccaaact
    12001 tcagctgcct gcgtttttaa cgttcgctca atatattgtt taagaatagc ctggttttgt
    12061 acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag ctccagtgcc
    12121 tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg cttatcctga
    12181 gtatcgaaaa catcaaaagg tgccatgtag ggatggcgat ttcccaggcg ttgtggtgac
    12241 ttccctgtcg caatatatgc catcagacca tgctccagaa aactcagcgt ggcatcaaac
    12301 atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag tgcactcact
    12361 attccgctga ataaatagac accgccgcat agatccgcaa gagaggtacc aacacgcact
    12421 ggcggagcat cagggtaccc tgtttccatc attatcccac tcattgcctg aatgatggta
    12481 tcgtaggcag gagcatcttt tagcggaccg gtatgtccga aacctgacga tgaagcatat
    12541 atgaggcgcg ggttgatttc ttgtaacctt tcccatgaaa accccagttt ttccattgta
    12601 cctaggcgaa aattctcagc taatacatca gcttgtttga gcatatttat aaatatactt
    12661 ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt aataaaactg
    12721 taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc accatgacct
    12781 ggtggctcaa ctttaattac ccttgcgccc atattataaa gaagttgagt tccgaaaggt
    12841 ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc tttgctttca
    12901 ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac ttcggcattt
    12961 atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa tccacaaggg
    13021 agccgtgacg acaaagccca gtacgctcac agccaatgac gctgtaccag tacgggtgta
    13081 gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaatg cgcctgccag
    13141 taccatcatc tgcaaatgtt cgctgttcaa atgacatgcc ataccaacga gaagcagcgc
    13201 cagtggcatc agaatcaact tcaggaaggt gttataagca atttcggcac tgaactcgaa
    13261 tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt tagccttcgc
    13321 aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca gcaccaggat
    13381 cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta atgcgctcag
    13441 attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca gaccaatagg
    13501 aatagtaatt gcgttaacaa taatagaaat aattgctacc actaaacctg ttgatacgga
    13561 atcaccataa ataggatcga gaactgcaaa ccccaggaat ccaatggtag gtgaacccgc
    13621 aattaatgca catacagctg cttctgcatg ggtacgttta aaaaatttgt agcaaccaaa
    13681 ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca gacgagtgtc
    13741 cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagagccg caggaagagc
    13801 gtagtttaat accagtttat taaatgcgcg ggcttgatct tctgaaaaag tttctcgtct
    13861 gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc caataaaaaa
    13921 tgttagcata atagtatttc cgataagggt cccctcacca tcgcataatg agttggcaag
    13981 aggagacgat gtcagtattt aattaccagc gacttgtttt gggttaagtt tggtaatatg
    14041 gccactttca gttcctgctg cagggtcgat aaccacatta ataatggtcg gtttgcgcga
    14101 ctgaataccg gtggttaaag catgacgaag ttcatctgtc gtggtgacgt tatagccaac
    14161 gccacgaaac gcatccatta atttgtcata ccttgcatgg tgcaacagat ccgttggtga
    14221 tggtgcacca gcgccactga gatcaacacc gtctcctctg tagatgccgc cattattaaa
    14281 aataacgatc gtcaccggca ggttatatcg acaaatcgtt tcaatttcca tcccactgaa
    14341 accaaaagca ctatcacctt caatggcgac aaccggagaa ccagaagtca cgctagcacc
    14401 gatggcatag cccataccga tgcccatgac accccaggta ccacaatcca gacgacgacg
    14461 tggtttatac atatcaataa tatttcgtgc attatccagg gtatttgcac cttcattaac
    14521 taaataaata tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg cattaaagta
    14581 atttaatggt tgagtatctg tacttaattt ttcatgcatt ttttgtgcat tttgctgctt
    14641 gtggatattt aaaatatcgc gccataccag tggagtcgta aatgtgtttt gtttcagttc
    14701 tgccagcata ccttgcatac tggatgcaat atcaccaacg actggcacag caatggggcg
    14761 gttgctgtca atttcctgcg gttcaatatc cagttgaata aactgtgtat ctgccgccca
    14821 tcctttttta ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca tgacaacgtc
    14881 agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg tatcttcaag
    14941 gatccctttc gccatagaca ttggcaggaa tggaatctga gcactttcaa taaattcacg
    15001 aagctgttca tcagcttgtg aatacgccgc gcctttgcca aggataatta atggccgttc
    15061 agcttttgct aaaagcgaaa ttgcgctagt gactgacttc gggcatggca ataatgctgg
    15121 cgacggattt tcaactttaa caatcgtggt taacgcttcg tctttttcca tcgtcgcggc
    15181 caggacattt gctggcaaat caagataaac tccgccaggg cgacccgata cagagacccg
    15241 gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa atgctgcttt
    15301 ggcatacggt tttgccgcat tcatttggtc cagctcttca taatcacctt gctgtaggtc
    15361 gacgatcgcg cggtcgctgg agccgctaat catgataatc ggaaaaccat ttaccgttgc
    15421 gttggccaat gcggtcaaac cattgaggaa tcctggcgca gaaactgtca ggcagatccc
    15481 cggtttttgg gtaagaaaac cgcttgccgc agcggcatag cctgccgact gctcatgacg
    15541 aaaaccaata taacgaatgc cttccgcctg ggcatggcgt gccatatccg tcacaggaat
    15601 acctacaaca ccataaatag tgtcaatatt attctgtttt aatgcttcaa cgatgatatg
    15661 cataccatct gtcatttgaa gttgatctga catgattaat aacctcgaaa tttttataaa
    15721 ttgggagcca gaagcccccg ttaatatcag atggcgtggt tttgcttcat tgcagcaatt
    15781 tcatcgtcgc tataacccag ctcctgcaat acagcagcgg tatgttcacc taatagcggc
    15841 gcagctttaa tatccggcgt aaaggcagag aatttcattg gacaaccaac ggtcagatat
    15901 tttccacgca acggttgttc cacttcaaca acactgccac tttggcgcaa agagggatca
    15961 agtgaaattt ctttcatact taaaaccggt gcacaaggaa tatcaaactg agtcaaatag
    16021 gccaccgctt catgtttatc aatagtgaca gtgtattttt cgatttcagc aaaaatatcg
    16081 aaaatatgtg gctgtcgggc atgggctgta ctgtatgccg gatcggtaat ccattctggt
    16141 ttgccgatgg ctttacaggt gttttcccag ttttgctcct gaatagtgaa ataaatatag
    16201 gcgttaggat cggtttccca gcctttacat ttcaggatcc agccaggctg accgccacca
    16261 cctgcattac caccgcgggg aactgcatca ccaaatgtac cattcggata ctgcgggtat
    16321 tcttccagat aacccaattt atcgagacgc tgctggtcac gtaatttcac gcggcaaagg
    16381 ttcaatacgg catcctgcat tgacatggtg actcgttgcc cacgccccgt tttttcgcga
    16441 tgcagcaaag cagcaagtaa accgatcagc aaatgcattc cggtgttgct gtcacccaac
    16501 gctgcagcgc ttaccagcgg cggaccatcc caaaaacccg tagtggatgc cgcgccaccc
    16561 gctgcctgag caacgttttc ataggctttt acattcacat aaggcgaaca ctcatcaaac
    16621 cctttgatcg aaccaaaaat cagacgtgga ttgatttctt gaatatgctc ccaggtgaag
    16681 cccatgtgat caatggcccc tggatgaaag ttctcgacta agatatcagc ttcgcggatc
    16741 agcttttcca ttacctcttt gccttccgct gttttggtat ttaactcaat agaacgtttg
    16801 ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg cagttggtga
    16861 cgcgttacgt caccaacgcc gggacgttca attttaataa cgtcagcgcc aaaccaggcc
    16921 agcatttgag tacaagatgg gccagattgc acaccggtga aatcgagaac tttaattcct
    16981 tgaagtggag ttgacatagc aatacctatt agttaattaa atatgtgtta atttagcgtt
    17041 tatttaaggc ataacgccct ctataaataa tctgcaaaat atcttcgtag cggcactcga
    17101 tatttttaaa ttcctgtatc aagtactgaa aattatttta tcgccacttc caattccttt
    17161 ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattaatt cgtggtaatc
    17221 agagagagtc gcgacatttg tgatgttatt cttacaactg taagaagcgc cggtgattta
    17281 caagtaagag tatgattacg ctttttaaag cgtaccataa agcataacat aataaatgat
    17341 ttgtgatgac acgggattat tgcggtttac tttgaggaaa ataaaatgcg tgtttataga
    17401 accgcgagcg ccaaataatt ttatgaccac aacctgtcgt attgtggtca taaaaaattg
    17461 tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcat tagttcacaa
    17521 acagtgcttc gctatcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct
    17581 cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt
    17641 taagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga agctcttcca
    17701 ttgcgccctt tttatcccct ttcgccattt tttcattggc aatttttatc gcggcttctt
    17761 tttcgggtgt tgctacataa ctctcagaaa ttccgacaga tgcattaatg acaatatatt
    17821 ggtcatcatt gagattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt
    17881 catctgacag caaagcggaa gcttcattgg tcagttcttt tgccttttcc ggatcgccat
    17941 gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga
    18001 tacgctctgc tgctgccgtt ttctgggttt gctgagtttg ctgttgtgcc gctaccggtg
    18061 cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa
    18121 ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc catcatattg
    18181 ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacaat
    18241 tgtagttatt atcgcaaagc ggcatttagt cttacagttg taggaatatg cctcctgtcg
    18301 cagagaacta ttcttatata ccacaaagag atgtcattta ttatattgat gtaaatattg
    18361 attaattata tgtaaataca gccataacat cgtatggttc tcagtgaacc attttcagga
    18421 taactttcta tgaaagtaaa cttaatactt ttcagcttat ttttattggt ctctattatg
    18481 gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa gtgagaggag ctataaagag
    18541 actgaaaaaa catcagcgat gacgaccaca cactctacaa aacttcagcc atcacaggcg
    18601 attttgttta agatgagaga agatgtgcca ccattaaacc tcacagaaga aatcacgcct
    18661 acttatccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct gtcacatggc
    18721 aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgagcc atcctgaccc
    18781 actatcgcct gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa
    18841 acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg aagtaataaa
    18901 tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta
    18961 cctgaccgat gtagccgctt acgcagacaa tacacagggc gagaaacatc cataaataaa
    19021 tcatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag
    19081 acaatcctta acctctggca atttttgcta agcatcaatg aaaacagcac gttaaatgca
    19141 acgctattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta gctgaaagca
    19201 gtcagccagt gataaagtag agccaccgca ttcatgctcc tcgtggttat gtcctgacta
    19261 gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac
    19321 cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt
    19381 ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg
    19441 ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca
    19501 cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa
    19561 acctggaact ttgtttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa
    19621 actttcgttc actcggcatg gcgctggtag aaaccggcat ggcatggttc tggcccgaca
    19681 gtcgcgtacg taaatggttt gatgttgaag ggttggataa ccttaaacgc gcacaaatgc
    19741 aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg
    19801 tgatgggact gtgccaacca atgatggcta cctatcgtcc acataataat cagctgatgg
    19861 aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc
    19921 tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg
    19981 attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa
    20041 ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg
    20101 taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg gaaggctacc
    20161 cgacagatga aaatcaagcc gctgcttata tgaacaagat tatcgagaaa gagatcatgc
    20221 gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat
    20281 cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa
    20341 cctcatgatt tttatagctt ttttcttaag ttttttcctt cgctgaatat ctaattatta
    20401 ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa agtaaataag
    20461 aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc gagcagttta
    20521 gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt cgctttgccc
    20581 ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt aattacgcgg
    20641 agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg
    20701 gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc
    20761 tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat gtttgctgct
    20821 gacgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg cctgacgaat
    20881 acggtcgtgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc
    20941 aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa attccagcga
    21001 tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag ccttcggcat
    21061 ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacgac ggcgtttgta
    21121 ctgttcggca atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa tcgccgccac
    21181 ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca gggcactgac
    21241 cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt agcttttcga
    21301 cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa ttgacggcgc
    21361 tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca gcacatcgta
    21421 gcatttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt gcgcggttgg
    21481 gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac gaatggcgcg
    21541 ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc
    21601 ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc
    21661 accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac caatagtgac
    21721 gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc agcgggaaat
    21781 ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg
    21841 ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg ggttacccat
    21901 gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtgat
    21961 gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc
    22021 agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacaat
    22081 ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt
    22141 tatcaatgga tttaaatgag gaactccact tcaaaaacag gaaatttttc tacgctaaca
    22201 agaaaatcac caccaatttt attaacatta taatttcaat aaatttacca tccagaagtg
    22261 cattatcaag agatacattg ctaatacacc atttatcttt taatcccctt ttaaatttat
    22321 ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt
    22381 taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga
    22441 tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga
    22501 actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg ccattttctc
    22561 gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc tggtgaacgt
    22621 acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag gcatcattac
    22681 cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga cggcgatccc
    22741 ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc tgaaaatccc
    22801 caaagcacag cgctggcgct ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc
    22861 aatagcgagt cattacgcaa ggtctgcgaa attatccgcg atgacattca cgccgatgcg
    22921 gtggcgatta ctaataccga tcatgtgctg gcctatgttg gcgtgggtga acataactat
    22981 cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta cggaaaaatc
    23041 atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat gctggtgatc
    23101 ccattgtggg agaaaggggt cgtgaccgga acgctgaaaa tttactactg ccacgcgcat
    23161 cagatcacct cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa
    23221 ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga gattcgcgcc
    23281 ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat ttcatcgtca
    23341 atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc
    23401 tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct gtatcaaatt
    23461 aaagactata ttgccattga gcaggcccgc tttggcgaca agctgacggt tatctatgat
    23521 attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggagaac
    23581 gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca
    23641 gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat cgttccaaag
    23701 gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct gctgaatgtc
    23761 catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct ggagccgggg
    23821 acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta
    23881 ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac
    23941 aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg
    24001 acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg
    24061 atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc cagttcgccc
    24121 ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac
    24181 tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc gggatgctgc
    24241 aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc gcgacggtaa
    24301 cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc acgccaatta
    24361 acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat
    24421 cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg tcgcattttt
    24481 tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt
    24541 ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg gtcagccgca
    24601 gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc atgaaagcac
    24661 caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggccta gtgcttaacc
    24721 cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt
    24781 tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg
    24841 aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca cgcgcggcta
    24901 atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc
    24961 ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct cgccagaccc
    25021 agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc acccatctta
    25081 gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg ctgcatcttt
    25141 gccgcaacgt ggtgaatatg ccgccgggta attcacaacc gcgcggcgat tttctctatc
    25201 acagcatttg taactgggtt taggataatt atgcccagcc gctcacccgc gagagcgtgg
    25261 cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacga
    25321 tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga
    25381 aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat agcgactatt
    25441 tttgtcgcgt tttccggcgt cagtttagtc tgacgccggg agagtacagc gcccgttttc
    25501 agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt tgtgcatctt
    25561 gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga
    25621 agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc accagttcga
    25681 cttcgcccat ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg
    25741 aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg
    25801 taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt tcagtgcgcc
    25861 cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg ctaaaatcct
    25921 gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg
    25981 cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga cgcgccagtt
    26041 cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga atacgcgggc
    26101 tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa cggctttcac
    26161 cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg gtaactattt
    26221 gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat acgcgcgggt
    26281 tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata ctgaagaaat
    26341 cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc accatgatcc
    26401 caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt
    26461 ggatctcgcc tttcacccat aagatctgat cgaggctgtg aaccatcggg atcatcaact
    26521 gggcgttgcc gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag
    26581 caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa
    26641 tattcagata aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag ataatcggct
    26701 tgtcgcccgc cgccagtagc acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg
    26761 cgctgtcgcg atccatatag agcatttcgg tacggaacag cccgacacct tccgcgccgt
    26821 tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata tcaatacgct
    26881 tgttatcacg ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat
    26941 ccgccagcgt ctgcgcgacc tgataataac cgctcaccgc gtcattcggg ttaatcgcca
    27001 gcacgccgca ctgggcgtca agcactgcag gttgcccggc ataacgggca atcgcatcca
    27061 gcggcaagcc actcagtacc gggatcgccg aggcacgggc cagaatcagt gtatgcgagg
    27121 tgcgcccggt tttctccaga atcatacccg caagattttt caaatcaagg ctcaaaaact
    27181 ggcttggggt taaatcttca gccaccagaa tggtcggttt ttccagcacc agattgttgc
    27241 gcggcttcag ttccggccag gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc
    27301 tgacccgctc gcgcagataa tcgctggcag aagcagatag tttggcgcaa acctgctcca
    27361 tattgctgat gatcgccgcc cccagtccct gatgctgttc tgtcatcagg cgacggatat
    27421 tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgagc gctgaggata gttttgcttt
    27481 cgccgtcacg ctcacgcaat tgctgattca gttgctcggc aagcgttgcc aggctgtgct
    27541 ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg aggctgtcgc
    27601 tttgtaacag ggtcagcgta cccacgccga cgccgcttgc cagcacattg ccgtacagta
    27661 aatccgggtt aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg
    27721 tcggctgaac gctgtcgctg tcgataaagc gcaccaggat gtactcttcc agcacgcgcc
    27781 gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta aataaggtgc
    27841 cagttccaat caacgccagc gagcttttag catcagcctt tgcgttctgg cgatggttga
    27901 taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac gccggacgag
    27961 catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt
    28021 aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa
    28081 taagggcaga taaaagttgc tccatctgca aaatgtcgcg gcaatcggca atcgatgccg
    28141 cgcaatgtcc gtggcgggtg gcaggcccca ttaccaatgt gggtacgcca gtaccggtta
    28201 agtgcaccgc tccaccgtcc gtgccgccgt tactgaacat atccgcctgc aacggaacgc
    28261 caatttctgc cgccacggtt tcaatccacg cggtaagttt tggcggcgca atcagcgatt
    28321 tatcgcttaa caccagcatc ggcccgttac caatctgacg atggttagcc gcgccataat
    28381 caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacgtccggc gacaccgcgc
    28441 gggtagcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc accagccaca
    28501 cttccgcagg cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc agatagcaac
    28561 tgaggcgatc atcaaaggct ttgcccatca cccgctggtg agggaggacc tgaaaagtgg
    28621 tatcgaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg tcataggtgc
    28681 gcgcgccaat gtccacgcgc atggcgctaa cgtcattccc ctgccggtcg ccgtcaagca
    28741 ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc tggcgggcag
    28801 ccatgcgcac gttgccaacc ggcagcacgt caatagcgcc ttcgcgggag atgctgcgca
    28861 ccataaatcc cacttcgtcc atatgggcgc agatcatcac cttcggacct gtcgattcat
    28921 tgaggcggat cagcaccgat cccagaccat caaatcgcac ttctttttgc aggcgagccg
    28981 cttcttccag caggatctgc cgcacttcct gttccgagga ggcgatcgca tctgcctcgc
    29041 tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca ctgttttcgg
    29101 catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt cgatgcgcac
    29161 gcccccttgc cctagcaaat aaatccccgg ctccacggtc agcaacatgc ctggctgtag
    29221 cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg acttcaatgc cgatagcgtg
    29281 accagtgtta tgaccgaaat agtcgccaaa tccagcctcg gtaatgaccc ggcgcgcggc
    29341 gtcgtcaatc tgctggcagc gcacgccggg gcgaattgca gagattgccg cgagctgtgc
    29401 ctgcaggaca atctgataga cgtcaaacag cgggtgagat tcggcgctca ccccttcgcc
    29461 attcaccagc aaggtgcgcg tcatatcagc agtagccctg atacagcgca ccaaaatcga
    29521 gagtgacaaa ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc ggcagcgccc
    29581 cgcgccagcc gctggcgaca atggtgtcaa aagaggcttt ttctgcgccc tgctggcgca
    29641 taaaccactc cagttcagcg gctatctcgc gctcgctcat ccccgcctga ataaagcggc
    29701 gaatatgctc cgctccgcga tcggcaatcc cacaggcgag gcggattatc tccacctcct
    29761 ctggcgtttt gatttgccgc agcacatccg gcgtggcgct aaccagtttc gcattgagtt
    29821 cagacttcca gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa ccgagcgttt
    29881 gcaactgctc atcggcaatg atttgattga cgatagtggt aagcgtgttc gttgcgtcaa
    29941 gcaaatgcag ctggtagcct tgcgcacggg cttccacctc aacgaaatag cgcgaatcta
    30001 ccagaatgtg cgcactttca cggctaataa ccacatagcc tgatccggtg gagatcccca
    30061 gatgcggctg tttgttctgc cgtgaggaga gaagcaccgc atccagttgt tgcaccttaa
    30121 gccagttgcg cagcgaagcg agtaatgtca tctggatttt ccttcttaca ggctatcgat
    30181 taacaatttg cctttgcgga acatcatcag acgcaggaac accaccatca gcgcagtaat
    30241 gattgccccc agcgcgatcc ccgccatata aacgcccaag ttagtgacca gcggccatgc
    30301 ccagatagcg gattccggga accattgcac tgcgcccagc cagacagctg cggttgagcc
    30361 gacaatcgcg ccgaccatat atgacggaat agcggtgatg gggctttcca gcgcaaacgg
    30421 aatcgcaccc tcactaatcc ccataaaggc caggaacatc gcggtcttac cctgcggata
    30481 aagttgagcg ttgaacaggc gtttgccggt taaacgacgg tcaataatgg tcgccagacc
    30541 cagaccaatc ggcggaataa cgatagcgat agaacgagcg gtgaccggta atacgtggtc
    30601 agtggtaaag ctgaaggcaa cgaaacctgc ggctttgttg atcgggccac caaggtcgat
    30661 tgctgtcgcc gcggcgatcc ccattgcgta catcagcgcg cccttctcac ctgcggcagt
    30721 cagcacagta cggataccgc cgttgatcca gccaccaaag ggagtgatga cgtagtacat
    30781 cgccagcatg acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt tgaacgctaa
    30841 caggaaatcc ggtaactgga ttttctggtt catccatttc acaaggtaac cggcaacgat
    30901 agaaataatc agcgcaccaa tgaaggtgga cggcaccggc gaagaggtcg cccattgcat
    30961 agtgctggga tcgaagttca gcagttgcgt cggctgggta gacatcaacc cgccgataaa
    31021 accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag caaacatcgg
    31081 aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa atttaagtaa
    31141 ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc cgatctcagc
    31201 aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat
    31261 aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca
    31321 gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg
    31381 cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg
    31441 ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc
    31501 gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat cttccggggt
    31561 aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg tcaggcgatt
    31621 ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt ctaccgccgc
    31681 ttcttccagc acctgagcgg ccataaaagt gtgagccagg cccattgggc aggcacataa
    31741 ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac tagtgaaatt
    31801 atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat
    31861 cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag aaaaattgaa
    31921 ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac aggaaggatt
    31981 tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag acccggatag
    32041 tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct
    32101 tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg cacgataccg
    32161 cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg
    32221 cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct gtcagccagc
    32281 gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc agctttcaca
    32341 attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc cgaaacatga
    32401 ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt cggcgcaaaa
    32461 tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg gaccagatgc
    32521 gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc gaccggttct
    32581 gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg
    32641 gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt
    32701 gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg
    32761 gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg aatgaccgct
    32821 tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca
    32881 atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt
    32941 gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg
    33001 ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca
    33061 attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa
    33121 gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt
    33181 attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat
    33241 gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat
    33301 agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta ttgcggtggg
    33361 gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat
    33421 cggcgcaccg gttccgccct ctgcgctacc tagacttatc gtagcattaa ttctcggtct
    33481 tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata tcgcccttgc
    33541 cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc
    33601 ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt tgatattttc
    33661 gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg cgccgttaat
    33721 ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt tttcactgcc
    33781 cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa ttgtcgcggc
    33841 gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc acgcgatgat
    33901 gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggaattct
    33961 gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt
    34021 ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta aacttgccgc
    34081 cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt
    34141 cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag cggcaataac
    34201 cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct
    34261 ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat
    34321 gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca
    34381 acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt aagagttatg
    34441 gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg
    34501 atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt
    34561 taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca aattacaaat
    34621 tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag atcgtgatta
    34681 ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata atggctatca
    34741 tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct ggcgcgatcg
    34801 tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc gtcctccaat
    34861 ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac cggctcttta
    34921 acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc
    34981 attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag
    35041 cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa
    35101 cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc agggcgatac caaaacttaa
    35161 cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa
    35221 tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc gaatgaatcc
    35281 ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc cggcagcaac
    35341 cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca gcgtcagata
    35401 agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg ccgcagctgt
    35461 agccatcatc accagattga caaaaccggc aatcgtcatg gcgatagcca catcccattt
    35521 ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga gcgaagagtg
    35581 caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca ggaagaccgc
    35641 ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca ggttaggctg
    35701 ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc cgccaatcac
    35761 tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag tcgcgatccc
    35821 cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac cgatcgccgc
    35881 accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa cccaatagaa
    35941 ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt taccggtggc
    36001 aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg cccaaacgac
    36061 aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg caaagttacc
    36121 gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt
    36181 acgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt gcctctaaaa
    36241 catagccttt gctatgtttc atgctatgct aaacgagaat gattaccaaa ttcatttaaa
    36301 tggattgtgg tgatttctct gatagaccaa gattatgatt acgaaaagat tgatgacttg
    36361 aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac
    36421 atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg tcattatagt
    36481 gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct
    36541 gcctcatatt tggagcaaat atggaccgcg tccttcattt tgtactggca cttgccgttg
    36601 ttgtgattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta
    36661 ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt
    36721 taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt gccaacgaag
    36781 ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag
    36841 tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac attcgcgtgt
    36901 tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt atgggcaaac
    36961 tggaatcctt taacgccgtc agctccctga ttctgggtca gtcagaaaac tttattgcct
    37021 ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct gccacggcaa
    37081 tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg
    37141 tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg ctgatcaatc
    37201 cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac gaaggtcaga
    37261 gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg
    37321 ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg tttgccaccg
    37381 ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac atcttctatc
    37441 cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg
    37501 cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca
    37561 cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct agtttccttc gctaacttct
    37621 cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg
    37681 tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt
    37741 caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca ttccccggct
    37801 tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc cttgcagata
    37861 ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa actccgcgac
    37921 cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca tcgcatccag
    37981 cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat cagcctgcaa
    38041 acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg caatccggaa
    38101 accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag aaaacgcctg
    38161 ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct taaacagacg
    38221 aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat tgactgaaaa
    38281 gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg attccagcac
    38341 ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa acttatccgg
    38401 ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac cttcaccctc
    38461 tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg ccgtgcgaat
    38521 ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct ctctgctatc
    38581 cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta actgccccaa
    38641 caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac catagcccat
    38701 atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa ccatatgctg
    38761 gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac ccggcaattg
    38821 atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg agcggataca
    38881 gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat caatgcgcag
    38941 gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac gaaagttggg
    39001 taggagcgtt aacggatctg ttagcgcctg caaatgccag cggcgattaa gccattcact
    39061 gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca gcaccgcgag
    39121 aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctgat tgtaattcag
    39181 aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa gattaccgac
    39241 cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa agtcgttttc
    39301 ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc ttaaggtaaa
    39361 tgcgagtgag atagtagaaa agcatgttgt aaatcagcac tgcggtgaaa aggcttagta
    39421 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39481 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    39541 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    39601 ccagctgcca gcgtaatatt gcgcaagcac acaacacgac aaacgtctgg ctgccgagca
    39661 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    39721 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    39781 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    39841 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    39901 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    39961 acacaaataa taagtttatt cgcatcgcga agcaaacagg ggccagcctg ttgccgattt
    40021 ttcatccaat gatgctttca ccaggcggcg ctaacccgga cgtagactgt attacaaaag
    40081 cggcaaaaag cagagacaaa aaacccccgc tttgcagcga gggttggaaa tttggtggag
    40141 ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct
    40201 atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga
    40261 atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac
    40321 aattttggaa aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt
    40381 gttataaaat gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag
    40441 aggatgacac cagatgaact tgccagactg accggctata gccgccagac cattaataaa
    40501 tgggtacgca aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc
    40561 cgactggttc acgtcaatga acaggttcgt gaatatattc gcaatgctga acgtccagaa
    40621 ggtcagggag aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca
    40681 ctggcgaaag agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa
    40741 gggattatcg gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt
    40801 acgcaataaa atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt
    40861 taaccgttgg accagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg
    40921 tcgtgccaga cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc
    40981 cttccataac acaccaatgt tgatggaagc tgaagagaga attgctgaat acgctcctgg
    41041 agcacgcgcc ccggcgtatc gacaaatcat caacgcgata gataatatga cagacattga
    41101 gcaggaaaaa gtcgcgcaat ttttgtcacg tgaaggaatt cgcaatttcc tcgcccgtct
    41161 cgatattgac gaatcagcat aaataaaaaa cggcaggata ttatctcctg ccgtttatct
    41221 ttttacacgc taattactgc tgattttcgc gttcagcaat aaaatccagc gctttgttga
    41281 tacgctcgat actgcgggtc ttaccaattg cgtgaacggt aacatccagt gctggagact
    41341 gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca
    41401 gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg
    41461 cggccagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt
    41521 tcgccgcatc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca
    41581 tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc
    41641 cgttacgggt atcgatattt tcctgctcaa tgtgccactg taagtgagta gcaacatact
    41701 ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg
    41761 cactggcaga tttgctgacg gcattcaaag tgaagtattt gatcatctct tcacgagtga
    41821 agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg
    41881 gcaaataacc gtcatcacga tactgcatta cgctgactgc cccgtgacgt ttggacagtt
    41941 ttttaccgtc atcgccattg atcatagaaa cgtgcgcgta aaccggcacc ggcgctttca
    42001 gggccttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa
    42061 cgtgggtgat ttccatatcc cagtcatcga caaccacaca gaagttatag gttggggaac
    42121 catcggtacg gcggataata agatcgtcca gttcctggtt gctgaactcg atcggaccac
    42181 ggatctgatc gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacacacg
    42241 gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct
    42301 cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagag caatagcatt
    42361 tataagcagt gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt
    42421 tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa
    42481 tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga
    42541 actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa
    42601 cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt
    42661 acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta
    42721 tcactcccgc ctgattcctc aatgtagttc gggtttctac cttacgcatt attgttttcg
    42781 ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg
    42841 aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat gcgactccac
    42901 acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    42961 ttcgatcccg tcatcaccca ccaactactt tatgtagt
//
LOCUS       DAJVPT010000023        41793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVPT010000023 DAJVPT010000000
VERSION     DAJVPT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQM47_001218"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQM47_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1479445.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OQM47_001219"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OQM47_001219"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1479446.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OQM47_001220"
     CDS             complement(3736..4092)
                     /locus_tag="OQM47_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1479447.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OQM47_001221"
     CDS             complement(4096..4512)
                     /locus_tag="OQM47_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1479448.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQM47_001222"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQM47_001222"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1479449.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OQM47_001223"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OQM47_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQM47_001224"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQM47_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1479450.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQM47_001225"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQM47_001225"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1479451.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQM47_001226"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQM47_001226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1479452.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OQM47_001227"
     CDS             10521..11504
                     /locus_tag="OQM47_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1479453.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQM47_001228"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQM47_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1479454.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQM47_001229"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQM47_001229"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1479455.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OQM47_001230"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OQM47_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OQM47_001231"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OQM47_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1479456.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OQM47_001232"
     CDS             complement(15306..15515)
                     /locus_tag="OQM47_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1479457.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQM47_001233"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQM47_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1479458.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQM47_001234"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQM47_001234"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1479459.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQM47_001235"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQM47_001235"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1479460.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OQM47_001236"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OQM47_001236"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1479461.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQM47_001237"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQM47_001237"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1479462.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQM47_001238"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQM47_001238"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1479463.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OQM47_001239"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OQM47_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQM47_001240"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQM47_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1479464.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQM47_001241"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQM47_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1479465.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQM47_001242"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQM47_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1479466.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OQM47_001243"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OQM47_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1479467.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OQM47_001244"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OQM47_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1479468.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OQM47_001245"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OQM47_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1479469.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OQM47_001246"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OQM47_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1479470.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OQM47_001247"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OQM47_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1479471.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OQM47_001248"
     CDS             complement(34461..34604)
                     /locus_tag="OQM47_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479472.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OQM47_001249"
     CDS             34605..34883
                     /locus_tag="OQM47_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479473.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OQM47_001250"
     CDS             complement(34930..37026)
                     /locus_tag="OQM47_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1479474.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OQM47_001251"
     CDS             complement(37026..37763)
                     /locus_tag="OQM47_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1479475.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OQM47_001252"
     CDS             complement(37760..38398)
                     /locus_tag="OQM47_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1479476.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OQM47_001253"
     CDS             complement(38512..38754)
                     /locus_tag="OQM47_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479477.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OQM47_001254"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OQM47_001254"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1479478.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..>41793
                     /locus_tag="OQM47_001255"
     CDS             41391..>41793
                     /locus_tag="OQM47_001255"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; functions in amino acid
                     biosynthesis; lysine sensitive; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1479479.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERD"
BASE COUNT        10424 a        10752 c        10225 g        10392 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgataccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atg
//
LOCUS       DAJVPT010000024        40008 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVPT010000024 DAJVPT010000000
VERSION     DAJVPT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..804)
                     /locus_tag="OQM47_001256"
     CDS             complement(<1..804)
                     /locus_tag="OQM47_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1479480.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDR"
     gene            1050..1676
                     /gene="gstB"
                     /locus_tag="OQM47_001257"
     CDS             1050..1676
                     /gene="gstB"
                     /locus_tag="OQM47_001257"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCS1479481.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1673..2787)
                     /gene="yliI"
                     /locus_tag="OQM47_001258"
                     /pseudo
     CDS             complement(1673..2787)
                     /gene="yliI"
                     /locus_tag="OQM47_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2898..3281)
                     /gene="bssR"
                     /locus_tag="OQM47_001259"
     CDS             complement(2898..3281)
                     /gene="bssR"
                     /locus_tag="OQM47_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1479482.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3494..4819
                     /gene="rimO"
                     /locus_tag="OQM47_001260"
     CDS             3494..4819
                     /gene="rimO"
                     /locus_tag="OQM47_001260"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1479483.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4866..6194)
                     /locus_tag="OQM47_001261"
     CDS             complement(4866..6194)
                     /locus_tag="OQM47_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1479484.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6202..8544)
                     /locus_tag="OQM47_001262"
                     /pseudo
     CDS             complement(6202..8544)
                     /locus_tag="OQM47_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8722..9633)
                     /gene="gsiD"
                     /locus_tag="OQM47_001263"
     CDS             complement(8722..9633)
                     /gene="gsiD"
                     /locus_tag="OQM47_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1479485.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9636..10556)
                     /gene="gsiC"
                     /locus_tag="OQM47_001264"
     CDS             complement(9636..10556)
                     /gene="gsiC"
                     /locus_tag="OQM47_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1479486.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10574..12112)
                     /gene="gsiB"
                     /locus_tag="OQM47_001265"
     CDS             complement(10574..12112)
                     /gene="gsiB"
                     /locus_tag="OQM47_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1479487.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12132..14003)
                     /gene="gsiA"
                     /locus_tag="OQM47_001266"
     CDS             complement(12132..14003)
                     /gene="gsiA"
                     /locus_tag="OQM47_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1479488.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13990..14955)
                     /gene="iaaA"
                     /locus_tag="OQM47_001267"
     CDS             complement(13990..14955)
                     /gene="iaaA"
                     /locus_tag="OQM47_001267"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1479489.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15159..16394
                     /gene="moeA"
                     /locus_tag="OQM47_001268"
     CDS             15159..16394
                     /gene="moeA"
                     /locus_tag="OQM47_001268"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1479490.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16394..17143
                     /gene="moeB"
                     /locus_tag="OQM47_001269"
     CDS             16394..17143
                     /gene="moeB"
                     /locus_tag="OQM47_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1479491.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17322..17984)
                     /gene="fsa"
                     /locus_tag="OQM47_001270"
     CDS             complement(17322..17984)
                     /gene="fsa"
                     /locus_tag="OQM47_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1479492.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18115..19014
                     /locus_tag="OQM47_001271"
     CDS             18115..19014
                     /locus_tag="OQM47_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1479493.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19020..21452
                     /locus_tag="OQM47_001272"
     CDS             19020..21452
                     /locus_tag="OQM47_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1479494.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21598..22413
                     /gene="ybiV"
                     /locus_tag="OQM47_001273"
     CDS             21598..22413
                     /gene="ybiV"
                     /locus_tag="OQM47_001273"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1479495.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22565..23830
                     /locus_tag="OQM47_001274"
     CDS             22565..23830
                     /locus_tag="OQM47_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1479496.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24071..25663)
                     /locus_tag="OQM47_001275"
     CDS             complement(24071..25663)
                     /locus_tag="OQM47_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1479497.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25882..26802
                     /gene="ldtB"
                     /locus_tag="OQM47_001276"
     CDS             25882..26802
                     /gene="ldtB"
                     /locus_tag="OQM47_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1479498.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26860..27978)
                     /locus_tag="OQM47_001277"
     CDS             complement(26860..27978)
                     /locus_tag="OQM47_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1479499.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27975..28442)
                     /gene="mntR"
                     /locus_tag="OQM47_001278"
     CDS             complement(27975..28442)
                     /gene="mntR"
                     /locus_tag="OQM47_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1479500.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28628..28756
                     /gene="mntS"
                     /locus_tag="OQM47_001279"
     CDS             28628..28756
                     /gene="mntS"
                     /locus_tag="OQM47_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1479501.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29028..30611
                     /gene="opgE"
                     /locus_tag="OQM47_001280"
     CDS             29028..30611
                     /gene="opgE"
                     /locus_tag="OQM47_001280"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1479502.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30659..31174)
                     /gene="ompX"
                     /locus_tag="OQM47_001281"
     CDS             complement(30659..31174)
                     /gene="ompX"
                     /locus_tag="OQM47_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1479503.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31227..31292)
                     /locus_tag="OQM47_001282"
     CDS             complement(31227..31292)
                     /locus_tag="OQM47_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479504.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31527..32414
                     /gene="rhtA"
                     /locus_tag="OQM47_001283"
     CDS             31527..32414
                     /gene="rhtA"
                     /locus_tag="OQM47_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1479505.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32713..33216
                     /gene="dps"
                     /locus_tag="OQM47_001284"
                     /gene_synonym="pexB"
     CDS             32713..33216
                     /gene="dps"
                     /locus_tag="OQM47_001284"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1479506.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33620..34366
                     /gene="glnH"
                     /locus_tag="OQM47_001285"
     CDS             33620..34366
                     /gene="glnH"
                     /locus_tag="OQM47_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1479507.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34505..35164
                     /gene="glnP"
                     /locus_tag="OQM47_001286"
     CDS             34505..35164
                     /gene="glnP"
                     /locus_tag="OQM47_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1479508.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35161..35883
                     /gene="glnQ"
                     /locus_tag="OQM47_001287"
     CDS             35161..35883
                     /gene="glnQ"
                     /locus_tag="OQM47_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1479509.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36144..38368
                     /gene="ybiO"
                     /locus_tag="OQM47_001288"
                     /pseudo
     CDS             36144..38368
                     /gene="ybiO"
                     /locus_tag="OQM47_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38365..39291)
                     /gene="rlmF"
                     /locus_tag="OQM47_001289"
     CDS             complement(38365..39291)
                     /gene="rlmF"
                     /locus_tag="OQM47_001289"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1479510.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39567..39827
                     /locus_tag="OQM47_001290"
     CDS             39567..39827
                     /locus_tag="OQM47_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1479511.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
BASE COUNT         9949 a        10571 c         9746 g         9742 t
ORIGIN      
        1 acggtcagtt ttcgccccca gcactacgga gattaaacgc atatcaccct gggtagccga
       61 agcaaccaga ttatatcccg cacctgccgt ggttcctgtc ttcatgccat caacattcag
      121 attgctgctc cacagcagac ggttacggtt aggctgacga attttgttga aggtgaactc
      181 tttctcttta tgaatggcgt actcttccgg cacatcgtgg atcaatgctt tacccagcaa
      241 tgccatatcg cgcgcggtac tgaactgccc cggcgcatcc aggccgtgca ccgtctggaa
      301 ggtagtgttg gtcagcccca gttttttggc ataaccattc atcagaccaa taaaggactc
      361 ctggctcccg gcaacgtaat ctgccagcgc aatacaggcg tcattaccgg actggataat
      421 cacacctttg ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga acattaccga
      481 tgaaccacgc agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc
      541 ggtgagttta atcttatcgg ccttaagcgc ctgcccaacc acatagctgg tcatgatttt
      601 agtcaggctc gcaggatcca gtttctcatc cgcattgcct tctgccagca ctttaccgct
      661 ggcgtaatcc attaaaatcc atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc
      721 ggccgcgaat gccgttgggg caaaaaggaa taaaaatgca gaacccgctg caagaccacg
      781 aaggagagag gagtattgcg tcataaatgc cacccgagta tccattcaaa aaaatacgtt
      841 atatcgccgt atgataagtt cggtgattaa taacgcacac acaaacttaa agaaaccgta
      901 agttggtaaa gtttttaaag tttatgcaat atcctgccgc ttcgctgctt ctggtgtcat
      961 ttttttgcca gctgttgctt aaaagttaac tttacttcac catcggagtc ctgttcagtc
     1021 tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg aataattcaa
     1081 ctaacgtaaa aaaagtattg ctgacgctcg aagaactgga actaccttat gagcaaattc
     1141 tcgcgggccg tgagtttggg ataaatcacg atgctgattt tctggcgatg aaccctaacg
     1201 ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa tcaaacgcca
     1261 ttgtccgcta tctggcggca cagtacgggc aaaaacgcct gtggatcgac tcaccggcac
     1321 gtcgtgcgga agcggaaaaa tggatggact gggcaaacca gacgctcagt aatgctcatc
     1381 gcgggatcct gatgggatta gtcagaacac caccggaaga gcgcgatcag gccgccattg
     1441 atgccagttg caaagagtgc gacgccctgt ttgccctgct cgatgcggaa ctggcaaaag
     1501 taaaatggtt ctctggcgac gagtttggtg tgggcgatat cgctatcgca ccgttcatct
     1561 acaatttgtt taacgttggc ctgacctgga caccgcgtcc aaatctgcaa cgctggtatc
     1621 agcaactcac tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt agctaattgc
     1681 gtgggctaac tttaagtaat tccccactgg actcgtcggt gagaacgtat aaataaccgt
     1741 cgggtccagt gcgaacatca cgaattcgct gccctctgtc cgttaaaata cggccatctt
     1801 ctgtcacttt gtcgccgttg acgctcatca caatgacatc tttatctttc agctcccaat
     1861 aaataatttt tgctgccact gggggaattt atcgctgtta tagaaggcca tgccgctcac
     1921 agcgggcgaa tctttccagt aaaaaacagg ttgctcggtc ccggcgacga tctccccttt
     1981 cgcttccggt atcttaaagc ctgaatagtt gattccccag gttgccagcg gccagccgta
     2041 gtttttgcct ttttgcggga tattaatttc atcaccaccg cgcgggccat gttcattcag
     2101 ccacagtgca ttactccacg gattcatcgc cattccttgc ggattacgaa tgccataaga
     2161 ccagatctcg gcgcgcgcac cggattcctt tataaaagga ttatcatccg ggatttcgcc
     2221 ctggtcggtc agacgcacca gtttgccctg taatttatcc agatcctgcg ccgtcgggcg
     2281 ctgattgttt tcgcccagag caataaaaag ataaccttta ccgtcgaata ccagccgccc
     2341 gccaaaatgg ttgccggtag acagttttgg catctggcgg aagacggtgc ggaagtcggt
     2401 cacttttgag agatcatcac ttaagcggcc ataacccaca gcagttccgg ctttgccatc
     2461 atcgccaact tcggaataac ttaaccagat gcggcgagac tgagcaaaat caggcgctaa
     2521 aaccacgtcc agcaggccgc cctgcccgtg cgcccaaacg tccggaactc cggaaagcgg
     2581 cgcagataat ccttttcctg cttgccagtg acgcaactcg ccgccgcgca gagtgattaa
     2641 cataccgtga ttatcgggta aaaaggccag tgcccaggga tggtcgagtt tgtcttgcag
     2701 tacttcgaca tttaccgttg caggagccgc ccagagagcg gaagaaagac aaataagggg
     2761 cacaaggaaa aaggattgtc gatgcataac gcctccggat tgactctggc ttaaagcgta
     2821 gtcagtggag gagataacaa attcattttt acaaaaactt aaacatgaag ggggagacgc
     2881 tttctccccc ttagttttca ggccttctca agcatggcgt gcttctgcag gctctggata
     2941 ctcagcgtta agctcatcag acaattttca agcttatcgg cgttgacggt aataacagtc
     3001 gggcaatcat ggtgcccact catcaaacat actgcggctg tcgctaatgc ttcttcagca
     3061 tgatgaagag cactccactc ttcctgatcc agatgaagat tcaaccgcag cgatttatcg
     3121 tgcagttcgc gattcagttt aaaaaagtta tctcgtagat gattgctttc gctgacggac
     3181 atgtatcctt ttgccttcct cagttgcacg tatgcagcga ggtcaacgcg tgcgtcgatc
     3241 aaccggttca gcagatcgat tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc
     3301 cagccttgtt gcaattaaat agtatggcta ttttttgagc aaaatgctgt ccaggatcaa
     3361 ttcttcacgc cgaattaaca aaatttacat ttccttcgcc tccgccagcg aatgagattg
     3421 cgatacaaat tttatccaga actctgtaga atccctgccc tgacctttcc tgtaactgaa
     3481 tattttttaa gctatgagca aagtaactcc ccagccgaaa atcggctttg tttcccttgg
     3541 ctgtccgaaa aaccttgtcg attcagagcg tattctcacc gaactccgca ctgaaggtta
     3601 tgacgtggta ccgagctatg acgatgcgaa catggtgatc gtcaacacct gcggctttat
     3661 tgacagcgcg gtacaagaat cactggaagc cattggtgaa gcgttgaatg aaaacggcaa
     3721 ggtaattgtg accggttgtc tgggggcaaa agaagatcag atccgcgaag tccacccgaa
     3781 agtgctggaa atcaccgggc ctcatagcta tgagcaggtt ctggagcacg ttcatcacta
     3841 cgtgccaaaa ccgaaacaca acccattcct gagcctggtg ccagaacaag gtgtgaaact
     3901 gacgccgcgt cattatgcct atctgaaaat ttcggaaggc tgtaatcacc gctgcacctt
     3961 ctgcattatt ccgtctatgc gcggcgacct gatgagccgt ccgattggcg aagtgttaag
     4021 tgaagcgaaa cgtctggtag atgcgggcgt taaagagatt ctggtgatct cgcaggatac
     4081 ttccgcctat ggcgttgatg ttaaacatcg tactggcttc cacaacggcg agccggtaaa
     4141 aaccagcatg gtcagcctgt gcgaacagtt atcgaaactg gggatctgga cacgtctgca
     4201 ctacgtttac ccttatccgc atgtggacga cgtcatccca ctgatggcag aaggcaaaat
     4261 cctgccgtat ctggacattc cgttgcagca cgccagcccg cgcattctca aactgatgaa
     4321 gcgtccgggt tctgtagatc gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc
     4381 ggaactgacc ctacgctcaa cctttattgt cggcttccct ggcgagacgg aagaagattt
     4441 ccagatgcta ctcgacttcc tgaaagaagc acgtctggat cgcgttggct gctttaaata
     4501 cagcccggtt gaaggtgcag acgccaatgc cctgcctgac caggttccgg aagaagtgaa
     4561 agaagaacgc tggaaccgtt tcatgcagct gcagcagcag atttccgccg agcgcctgca
     4621 agagaaagtg ggccgtgaaa ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat
     4681 tggtcgcagc atggcagatg caccggaaat cgacggcgcg gtttatctca atggtgaaac
     4741 caacgttaag ccgggtgata tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt
     4801 gtggggtagc cgggtttaat taattcaggc cgggcaaaat catttgtccg gcctgcgaat
     4861 aatgattact gccagctaat ctgtgccctg ccgtttttct tactcttata cagccgctca
     4921 tccgccagca ttttcaagcg ttcgacggta gtacattcat ttgaatgcgc tacaccgata
     4981 ctggtgctta aattaatgct tcccacctgg aaatgaacga tttcttttag tatcgatagc
     5041 aactcttcca gcgtgccgcc agtataaatc accgcaaatt cgtcacctcc gaagcggtag
     5101 actttacctt tatctaccgg gatctgtgat ttaaggattt tcaccacttt catcaacact
     5161 tcatcgccca gcaaatgacc gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac
     5221 agacacaaat aaccgccttt ttccggcagc gcttttaaat cttcatcaaa ggattttctg
     5281 cccattgctt tggtcaggtc gtccaggaac gccgagttat acatactgta gagttttttg
     5341 acatcggcag ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaatg
     5401 atggcttctt tctccacaat accacgatta atcactctgc gtaccagata gaggcaactg
     5461 gcacccaata tcactgccac caatgcggca aagaaaataa tcaatgtaca aacccgcaaa
     5521 taaacatctt ttttatacgc aaatacgtac aatccggtcg gcaaataggc gaagtgctcc
     5581 atcgcttccg aaacggtgaa gatggcgttt ttagcattat attcatcacc ggtcaccggt
     5641 ttaccgttct taaatagaga gaacatcagg ttctcgctgt ccatcaccag cgattttgac
     5701 agtaaagaaa aacgctccag accaagcaaa aatatcagac tacgattgtg agttaccgga
     5761 tagaggtaat agacgaaggt tttaccgtca aaagagataa accctgtatt tttgacatgt
     5821 cgatacttca caacctgcat aatatctgca caataatttt tctcgaactc ctttacctga
     5881 atacattcgt cactggaaat cagataagga ccgacatcgt acaaataaga gggggaaacc
     5941 gagtcggcgt aagctgtgcg caaaccaacc aacagctgtt cctccgtggg atttagcgtg
     6001 ttgataagat caattgccac gctacgccga tattcagcaa tatttgccac cgcaaaagca
     6061 gtatcaataa cggactgttt caggctatac acccgttcct ttaccatttg agtgtagatc
     6121 ccgcaggcaa ctgctgaaat catgataaaa atcagcagac tgactgtaag tacgaactta
     6181 ttgattctgg acatacgtaa actactcttt tactaatttt ccacttttat cccaagcgga
     6241 gaatggcatg ggtttctggt acagataccc ctgagcctga acacatccta atttaattaa
     6301 ttcgcgcgca acgtcttcgc tctctacgcc ttccgcaatc accgggatcc ggttatagcg
     6361 tgccagatcg aaaataatgc gcacaatttt tcccgcagta ccgaaaatat cgccggtaaa
     6421 ttgcgagtcc acctttatgc tgtctggatt aatttcataa aaacgtttca ggtttgacaa
     6481 accggtgcca aagtcatcaa tcgatatccg taccccttgt ttacgcagtt gttcaatatt
     6541 gagataaaaa tgttcattaa ttcgctcgat cacggtttca gtgatctcaa cacagaggct
     6601 gttcgccttc aggccatttt ctttcatgat ggtggtaaaa cgctcatgaa agtgcggttc
     6661 gtttaactgc cagggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg
     6721 ttgactaaaa tgtctgaatt aacccattgc cgttttaata atctgataac ccagctcatt
     6781 gataaacccg atatcttcag caatggtaat gaacttcagc ggtgaaatga tcccccgctc
     6841 cgcagaaacc caacggcaca acgcctccgc gctcagaatg gcgcgggtgt taatatcaac
     6901 gatcggttga tagacgagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc
     6961 aatccagata ttctcttcta tttcgttgag tttcgcgtca tcacagaaaa taaactcgga
     7021 gaacatatgg tcacggatac tggaaatcgc cagtttcagt ttttctgcgt aactccaggc
     7081 gttttccccc tgcaaaggtt cacaaatggc agacttatgg atgcgcagat tgcggtagcc
     7141 aaagctgctg tcaaaatcgt ttaccatcgc tattttacgg gtaaatgtct gtacattttc
     7201 ccgacacaga agtaaataga gatcggcatt gaatgtcacg atatcgcagc attgcgcata
     7261 tttctcggca attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa
     7321 caccgcctcc acggcttcca gaccacacag tttgatgatc cccacagcca gatactgatt
     7381 attgttggac tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat
     7441 cagtgagagc aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaatt
     7501 tttgatgctg ataatctctt tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc
     7561 cgttgagtcg ggcaaaaacg ccagcgtatt aaccagctcg gtcatttcag aaaattgccg
     7621 acggaacaca cgagtggtca tgaaatatac caccagcaac acgataataa tgaacgcaaa
     7681 cggtagataa aaaagtttat ctgatgcgtt caataaatta ccgccaggat aataaaaata
     7741 gatttcgaac aatgcattat ttggcactgc tgatgtcttt gcaatatact ccgtatcgac
     7801 agaatcaata atattataat taccttcgct attataaata atcagttttt cacgagcgag
     7861 ttgattcttg gcaatgatct catgaccttg tttcaaaaat agaacttcat cattagcgct
     7921 gatttgatca agatactcgt tcagataccg ccagtttaaa tcgacatgaa ttttatctac
     7981 cgctaacaaa tcgtgatctt tgccgttgat acgtagcgta aaagagatac attttttacc
     8041 cggacgatca aagcaattgt atgccggatt ccagctgttg ttagagtcat ctgaagccac
     8101 cgaccacggt cgtttcgatg ggttaaaatc ggcaaaaata acccgccctg actcgtcgac
     8161 aagcggtcca tttacttgtt cctgatgata aacaacgatt ttatcattcg ccctccgcga
     8221 aaagagataa tatttattat catcaagtac cagtcccacc gagtctaaaa atggcatcat
     8281 ttccattttc agaatcacgg cgcgttgaat ctcctctgta ttttttaata aatacagtga
     8341 gtcctttaat gtcagggccg aacgttcaat atctttaaag aaatagccaa cgtttttctg
     8401 aataagttca aagcggctat cggcacgttt taccgcgcgt tccgatacca gttgatagtt
     8461 aatcatgaaa aaaagaccaa taaatgacag tgctgccaat aaaaataaaa ttatcagcct
     8521 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
     8581 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
     8641 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
     8701 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
     8761 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
     8821 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
     8881 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
     8941 gccaatgcgc atggtgaaaa acaccacgat agaagagacg gtcccaggca ggatatgacg
     9001 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     9061 tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac gcggggatgg aaaaaatggc
     9121 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     9181 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     9241 gccttcataa tatccagcca acaagcccag caacgtccct atcgccacac cgataaacac
     9301 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     9361 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     9421 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     9481 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     9541 ccagaattca tgccacggtg tacgtacctg gtcaggtttg accagtggca tggcgtttaa
     9601 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     9661 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     9721 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cagagtcaac gagtaagcgt
     9781 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     9841 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tgcgtagccc gtgtttgagg
     9901 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     9961 acgtcgacaa atgacgcacg ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc
    10021 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacagtagg cagccagccc
    10081 agttcaacgg agaatacctg aattaaaaac atccccagcg caaatgccgg aaacgagatc
    10141 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
    10201 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccat
    10261 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
    10321 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
    10381 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgggcccg
    10441 gcaatcaatc gcgccggatc gccgggcagc atgtggacaa ataaaaacac cagcaccgag
    10501 acgataaaca gcgtcggaat caaccccagt aatcgtttga taacgtaata aagcattcca
    10561 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggta tctggcatga
    10621 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
    10681 tccacggcga ttcttgccag atgatatcct gcgccgcctt atataagcgg gtcttttccg
    10741 ccggatcatt cgttttcagt gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa
    10801 acgcggtatt aaacagcgtc ggtggccagt tttgcgaggc aaacagcggc gatagtgccc
    10861 agtcggcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccactctctt
    10921 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
    10981 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
    11041 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
    11101 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
    11161 tacttggcgg taccacacca gtagctggag ttgcatagcc cgcaaaggca actttcacca
    11221 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
    11281 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
    11341 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
    11401 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
    11461 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
    11521 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
    11581 tctccttgcc atatttttcc agcgctgtcg gtgaaatcat cgcggtcgcc ggatgggcaa
    11641 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
    11701 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
    11761 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
    11821 atttaattcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
    11881 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
    11941 acgatttcgc tacggcctga gataacgtgt cattcgcgtc atacggatcg agcgtggtga
    12001 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
    12061 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
    12121 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
    12181 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
    12241 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
    12301 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcac
    12361 cgtgaaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
    12421 tctaccacag ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
    12481 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
    12541 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
    12601 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
    12661 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
    12721 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
    12781 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccaggtga caaggtatcg
    12841 attcgctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
    12901 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
    12961 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
    13021 ccgctgcgca aagggaaacg ggtgacaaga ttacgcaccc gtaaaacagg ttcgccatcc
    13081 accaccgttt tctgctcgat ggggggttcc tgtttcgctg gatgttcaag cgatatcaat
    13141 gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac agcagctaac
    13201 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
    13261 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
    13321 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
    13381 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
    13441 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
    13501 tgatgcgaat aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg atccagcatc
    13561 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
    13621 gcaatctgtt cacccacagt aaatactggg ttcagcgatg tcatcggctc ctgaaatatc
    13681 atcgccatat ccgcaccgcg cacatggcgc atttgtgcag cgctctgctc gctaagttca
    13741 atcacatcac gactgcgccg ccgcaacagc attttatcgc actgtactaa accgcccgcc
    13801 tgttccaaca gacgcatcaa tgccagcgca gtcactgact taccggagcc ggattcgcct
    13861 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
    13921 ttctgctggt cctgcataaa ggcaatatta agattttcaa ccgccagcac attaccggca
    13981 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
    14041 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
    14101 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
    14161 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
    14221 gatgtcatat gccgccagcg cgcggatgaa gacttcgcct gtgccggtac aagaaaccgc
    14281 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
    14341 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccagattgc cgtctaaatc
    14401 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
    14461 gtcgaggact gttgcccctt cctcgcgcgc tgccagtagt tgttcataac gcaaaggcgt
    14521 ggagaaaatc tccggtgaga cgcgctccat gccatgagca aacgcaaaat tttctgcccc
    14581 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
    14641 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
    14701 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
    14761 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
    14821 ggcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcga tgtagcgtaa
    14881 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
    14941 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
    15001 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
    15061 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tatggcgttt ttctgacata
    15121 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
    15181 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt taccccactg accacccagg
    15241 aaacgctgcc actggttcag tgttttggtc gtattctggc gagcgatgtc gtttcgccac
    15301 tggatgtccc ggggtttgat aactccgcta tggacggcta cgcggtgcgt ttagccgata
    15361 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccataccatg
    15421 gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
    15481 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
    15541 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
    15601 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
    15661 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
    15721 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
    15781 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
    15841 acgatccaca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
    15901 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaatgatt cttgaagagc
    15961 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
    16021 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
    16081 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
    16141 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
    16201 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
    16261 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
    16321 gcgatcgcgg caatgtggac gtgcgcgaat gggtggaagt agaaccgttt aacgcgttgt
    16381 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
    16441 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctggt
    16501 agtcggcctc ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg ccggtgtcgg
    16561 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
    16621 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
    16681 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
    16741 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
    16801 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
    16861 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
    16921 tagccgtttg tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga tggcaccgtt
    16981 gataggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
    17041 caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
    17101 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaagcct
    17161 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
    17221 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
    17281 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
    17341 aacgctccct gccagtcctg ctcaaacttc gtcacagcgg cttccaccgc cggatagcta
    17401 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatccggc cagtaagcag
    17461 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
    17521 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
    17581 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag cagcccttgt
    17641 gccgcgccat ataccgcggt tcccagcgtc ggaatccctt ccgcttttaa catcttaatc
    17701 gctgccagcc cctcggctgt caccggaact ttcaccacga tatccgcaat aatagaacgc
    17761 agcttaagcg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
    17821 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
    17881 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
    17941 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    18001 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    18061 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc aactatgatt
    18121 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    18181 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgtacgcag
    18241 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    18301 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    18361 gagcatctga cggcgttaac cgactgctgt ccgacacagg cgttaaccgt gtgtggtgaa
    18421 gtgaaaagcg ttgaggagat catggcgacc gttctgcgcg ataaaccgtt ttacgatcgc
    18481 agcggcggcg gcttaacgct ttcgggtggt gaacccttca tgcagccgga aatggcgacg
    18541 gcgctgctgc aagcctgcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    18601 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    18661 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagggtgctg
    18721 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    18781 cagggcttta atgccgacga aacctctgta aaagccatta ccgattttgc cgccgacgag
    18841 ctgcacgttg gcgaaattca ttttctgccc taccacacgc tgggcatcaa caaatatcac
    18901 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga gctgctcgat
    18961 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    19021 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    19081 tgcatattgt gaagccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    19141 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    19201 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    19261 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    19321 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgtgttctgc
    19381 atgaagtgtg cccgtggtgg cgcggtcaga cggtacagga tcgctgctat ggcatgttta
    19441 ccgatgagca aaaaggtctg ctggcgaccg gcatcattaa agcggaaggc aatatgacct
    19501 ccggcgatgc gcacctggcg gtcaatttcc cgctgctgct ggaaaaaggg cttgatggtc
    19561 tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtgctg gaagatttgc
    19621 acggcgaaca gttcctgaaa gcgattgata tcatgctggt ggcagtcagt gaacacattg
    19681 aacgtttcgc tgccctggcg cgtgcaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    19741 atgaactgct ggcgatggca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    19801 tctggcaggc gctgcaactg tgttacttca tccagttgat tttgcagatt gaatctaacg
    19861 gtcactcggt gtcgtttggt cgtatggatc agtatctcta cccgtactat cgccgcgacg
    19921 ttgaactgaa ccagacgctg gatcgcgaac acgccatcga attgctgcat agctgctggt
    19981 tgaaattgct ggaagtgaac aaaatccgct ccggctccca ctcaaaagcc tctgcgggaa
    20041 gtccgctgta tcagaacgtc accattggcg gacaaaatct ggttgatggt caaccaatgg
    20101 acgcggtgaa tccactctct tacgcgatcc tcgaatcctg cggtcgcctg cgttccactc
    20161 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa cgatttcctc gacgcctgcg
    20221 tacatgtgat ccgctgcggc ttcgggatgc cggcattcaa caacgacgaa atcgtgatcc
    20281 cggaatttat taaactcggt attgaaccgc aggacgctta tgactacgca gcgattggtt
    20341 gtatagaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    20401 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    20461 tgttcctgcc acaagaaaaa gcgttgtcgg caggtaactt caacaacttc gatgaagtga
    20521 tggacgcgtg ggatacgcaa atccgttact acacgcgtaa atcaatcgaa atcgaatatg
    20581 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    20641 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    20701 gcctgcaggt cggtattgcc aacctcggca acagcctggc ggcagtgaag aaactggtgt
    20761 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    20821 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    20881 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    20941 aacagtacca taacccacgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    21001 cgtcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaacg ccggacgggc
    21061 gtaaagccca caccccgctg gcagaaggcg ctagtccggc ctctggtact gaccatctcg
    21121 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    21181 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    21241 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    21301 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    21361 tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct ctctccagac gctcaggacg
    21421 atatcatcgc ccgtactgaa catatgctgt aatctcttcg gcccggctgc ggcgtcgccg
    21481 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    21541 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    21601 agcgtaaaag ttatcgtcac agatatggac ggtacttttc ttaacgacgc caaaacgtac
    21661 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    21721 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    21781 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    21841 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    21901 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    21961 tttgtcgcac tgatggcaaa acactaccat cgcctgaaac ctgtaaaaga ttatcaggag
    22021 attgacgacg tactgttcaa gttttcgctc aacctgccgg atgaacaaat cccgttagtg
    22081 atcgacaaac tacacatagc gctggatggc attatgaaac ccgtcaccag tggttttggc
    22141 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    22201 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    22261 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    22321 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggct
    22381 gtgctggata acacatcccc ttttaacagc tgacctctct accggagcca tgcgctccgg
    22441 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    22501 ttataacttg tattgtcaca tgcaaattag catagtttcg aatgaaagtt aatgcaaggt
    22561 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    22621 tcagatgaag cacgcgctgc gggcgcagct tggcgatgtc cagcagatct ttaatcagct
    22681 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    22741 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    22801 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    22861 gctaggctgg gatcagtcga tgctggacta tctgaaccgc aaccgctttg acgaggtcta
    22921 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    22981 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    23041 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    23101 tgtgagcgtc atctaccctg accgtatccg ccgccttccg cccggaacga cctccaaagg
    23161 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagca
    23221 cgttttcgcc aacgtcttta atggcaatct ggcgaaatac gatccctggc atgcggcaca
    23281 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    23341 attccagggc tggactgcgc tctcggatat gctgcctggt caggggttgc tgcacgtcgt
    23401 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    23461 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    23521 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    23581 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    23641 gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    23701 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    23761 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat
    23821 ggatgtttga gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt
    23881 actcagattg ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatccg
    23941 gcctacaaag tcctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag
    24001 cgcatcaggc tattttgcat ttgtcatcag ccgtcgtcac cccgacagta tcggggtgac
    24061 ggataatcac ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt
    24121 cgatcacgcg ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac
    24181 ggtcgtgtga aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact
    24241 cgatggattc catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct
    24301 gcatcattaa cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg
    24361 gctttttgat gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct
    24421 cgtcatcgcc ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa
    24481 actcatattc gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg
    24541 tgccgctgtc cggttgcaga tcgcccacca gcgttttcag cagcgttgat ttaccgacgc
    24601 cgttggtacc cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa
    24661 acagtggacc gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca
    24721 gtttcttatc ctgttcaaaa cggataaacg ggttctgacg gctggaggct ttcacctctt
    24781 ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt
    24841 tggcgctaaa gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttggcgt
    24901 tatcggccag cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac
    24961 ccggataaac gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa
    25021 ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt
    25081 ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat
    25141 ccggctcagc aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt
    25201 cactcatcgg gccgtagtgc tgttccactg gaattcccac gccaagcaac agttcaccgg
    25261 cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt cacttccaga tcggccactt
    25321 tatagccgtc ttcttcactc atttccggca aagcatagat gcggtcgcgc tcctgcttca
    25381 cttcccacaa ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg
    25441 caaactgatc ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca
    25501 gcgtcggctc aaggtcgccg ccgagaatct tcataaaggt ggatttacca ctaccgttcg
    25561 cgccaatcag gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt tcaaacaacg
    25621 gcttactgcc gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag
    25681 agatatgaca aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa
    25741 ggtctacact caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga
    25801 gctggaatga acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc
    25861 aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc
    25921 gctgttgtcg gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg
    25981 agtcgcctgg ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg
    26041 gagtattttg ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt
    26101 gtggatacct tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg
    26161 ccggataccg ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat
    26221 ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat
    26281 acgcctatca actggaccac caaagttgag cataagaaag caggcccgac ctggacgccg
    26341 accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac cgctaccggc tgtcgttccg
    26401 gcaggtccgg ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc
    26461 catggcacca acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg
    26521 cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt
    26581 attgatgagc cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat
    26641 aacccgctgt ctaccaccga agcccagttt gaaggtcagg aaatcgtgcc aattaccctg
    26701 acgaagagcg tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa
    26761 gcgatcaaaa atcgttccgg gatgccggtt cgcctgaatt aatcgtcaaa acgtaaaaca
    26821 aaggcggact cataatccgc cttttttatt tgccagacct taccaggccg ggatcataac
    26881 taacaaaacg tatcccacca gcgccgccca caacagcatc ggtattgaat agagatggaa
    26941 acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc
    27001 tggtaataaa ccaaagcccc ccacgtttac cgcccatgcc agtaataaag acggaggtac
    27061 ataattgagc agcaaaatgg tactcggcac gttactgatc acctgcgata aaccgactgc
    27121 cgttaaccat aacccgggtt cagataaatg actcacgtta cccaacacgc cttgcaacgc
    27181 tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcaacgt
    27241 ccagtccaca ctcaacacca cgcgacgcgc cagcagcaca aagcccgccg ccacaatcac
    27301 cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag
    27361 acaattccac accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag
    27421 cgcctttcca gggaaacaac accagcacaa cagcaggagt gtcagcatca ttgcgccagc
    27481 catcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag
    27541 aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc
    27601 aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg
    27661 aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag
    27721 caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag
    27781 cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt
    27841 gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa
    27901 gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg
    27961 taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc
    28021 gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg
    28081 acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc
    28141 agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc
    28201 atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca
    28261 gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg
    28321 taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc
    28381 tcttccacgt tcactaactg cgtcactttt tttgctattg gcgtacctgc gcgacgactc
    28441 attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta
    28501 aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct
    28561 gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg
    28621 aggtcttatg aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt
    28681 acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa
    28741 accttcagat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt atgcaggcat
    28801 aacgcgtttt ggtctgaaaa aaaccacttt ttgtcggatt tgcaatcccc ttcgcaaaag
    28861 atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt
    28921 atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga
    28981 cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc
    29041 tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt
    29101 tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg
    29161 cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg
    29221 tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc
    29281 ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc
    29341 tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa
    29401 tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt
    29461 gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg
    29521 gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg
    29581 tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag
    29641 atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggta gtgatcggtg
    29701 aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg
    29761 ccagcagcgt taacggtttg atatttgctg actacattgc tgccagtggc tccacgcaga
    29821 aatcgcttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact
    29881 ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa
    29941 tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc
    30001 tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga
    30061 ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg
    30121 gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga
    30181 aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt
    30241 acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc
    30301 tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc
    30361 aaaatttcca ggtgcctttt atggtcattt ccagcggcga taaagcgcat cgggtgatta
    30421 aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag
    30481 cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca
    30541 tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc
    30601 cgaaacctta aaaacaaaaa tccgccccga gaggcggatt tttatatcac caaagtgatt
    30661 agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct
    30721 gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt
    30781 aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt
    30841 taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg
    30901 gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc
    30961 ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc
    31021 ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt
    31081 aaccgccagt tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg
    31141 cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa
    31201 gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta
    31261 acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca
    31321 ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt
    31381 cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa
    31441 attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt
    31501 aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt
    31561 taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta
    31621 agtcactttt tcctctggtg agcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa
    31681 cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt
    31741 taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta
    31801 ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg
    31861 cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc
    31921 tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc
    31981 tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag
    32041 cggaacatgg ccctgcgacg gtagcaattg gttcgttgat tgcagcgtta attttcgtgc
    32101 caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc
    32161 tggctgtcgt tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc
    32221 gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt
    32281 ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta
    32341 tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat
    32401 tagacattaa ttaaatttac atttctgtat ggttatgcat aaccatgcag aatttctcgc
    32461 tacttttcct ctacaccgta tttatatatc gaattatgca aaagcatatt tattccgaaa
    32521 attcctggcg agcagataaa taagaattgt tcttatcaat atatctaact cattgaatct
    32581 ttattagttt tgtttttcat gcttgttacc actattagtg tgataggaac agccagaata
    32641 gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga
    32701 ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat
    32761 acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag
    32821 gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc
    32881 gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat
    32941 ctggatacca tggcagaacg tgccgtgcag ctgggcggtg tagctctggg gaccactcaa
    33001 gttatcaaca gcaaaacccc gctgaaaagt tacccgctgg acatccacaa cgttcaggat
    33061 cacctgaaag aactggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt
    33121 ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat
    33181 aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgctgatg gtgcagaact
    33241 ttagtacccg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca gcccacctca
    33301 acgcacttat ttagtgcatc catctgctat ccccagctga ttaagtaaat tttttgtatc
    33361 cacatcatca cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa
    33421 tcactgtgtt gagtgcacaa ttttagcgca ccagattggt gccccagaat ggtgcatctt
    33481 cagggtattg ccctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaatgag
    33541 ttgcaaaatt ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg
    33601 atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct
    33661 ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc
    33721 cttcgttccg tttgaattta aacagggcga taaatatgtg ggctttgacg ttgatctgtg
    33781 ggctgccatc gctaaagagc tgaagctgga ttacgaactg aagccgatgg atttcagtgg
    33841 gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac
    33901 cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt
    33961 gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt
    34021 tgctgtgaag agcggtactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga
    34081 tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga
    34141 cgccgttctg cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca
    34201 gttcaaagcg gtaggtgact ctctggaagc gcagcaatac ggtattgcgt tcccgaaagg
    34261 tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac
    34321 ttacaacgaa atctacaaaa aatggttcag tactgaaccg aaataataac gctacacctg
    34381 taaagcgcac tggcagttcc ctctcccccc tggggagagg attagggtga ggggcgcaaa
    34441 cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca
    34501 acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc
    34561 caaaatgacc ctgtggattt cggtcctcgg tctggctggc ggtctggtaa tcggattgct
    34621 ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat
    34681 tgaagtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc
    34741 gatggcgttt aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat
    34801 caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta tccacaaagg
    34861 ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat
    34921 tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat
    34981 taaagacacc tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga
    35041 aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct
    35101 gattattacc ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct
    35161 gtgattgaat ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct gcacaatatc
    35221 gatttgaaca ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa
    35281 tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cctccggcga tctgattgtc
    35341 gatggcctga aggtcaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt
    35401 atggtgttcc agcagtttta cctcttcccg catctgacgg cgctgggaaa cgtcatgttt
    35461 ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg
    35521 ctggcgaaag tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt
    35581 caacagcagc gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt
    35641 gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag
    35701 gatctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag
    35761 aaagtagctt cgcggctgat ctttatcgac aaaggccgga ttgcggaaga tggcaatccg
    35821 caggtgttga tcaagaaccc gccgagccag cgcttgcagg aatttttgca gcacgtctct
    35881 taataagaca cattgcctga tgcgctacgc ttatcaggcc tacaggatat ctggcaactt
    35941 attaaaattg catgaacttg taggacggat aaggcgttca cgccgcatcc ggcaaaaaag
    36001 cccgcacgtt gtcagcaacc tgcttaatat cccttcctcc ctttcacccg aaagggaggc
    36061 acaccagatt cctctcattt aaaatcgccc ctcccccagc atctatactt atctttttgc
    36121 tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg
    36181 gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca acggactcaa
    36241 cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg gcgcactggc
    36301 ggatgtgctg gataacgaca cctcgcgtaa agagttgatc gaccagttgc gtactgtcgc
    36361 cgcgacgccc cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca
    36421 aaccgtgctg caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg
    36481 cttcgggcaa ctttatcgca atatcaccgg ctccccgcat aagccattta atccacaaac
    36541 cttcagcaat gcgctgagcc atttttcaat gttagcggta ttagtgtttg gtttttactg
    36601 gctgattcgc ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg cgaggcaaaa
    36661 aaatcgtgag cgcagcaact ggttgcagct tccggcgatg attattgggg cgtttattat
    36721 cgacctgctg ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa
    36781 tgcaggcagt cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat
    36841 tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg agctgcgccc
    36901 gttcacgatt caggacgaga gcgcccgtta ctggggtcgt cgcctgagct ggttaagcag
    36961 cctgattggc tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca
    37021 gataggtgcg ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct
    37081 gatcttccga aataaaaaag agattaccca gcatatgctc aactttgcgg agcattcgct
    37141 ggcttttttc agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc
    37201 ctattttatc atgctgtttt tcttttcgtt gttcgatccg ggcaacagtc tgaaatttat
    37261 gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat
    37321 gttttcccgc tggctggcga aaaccatcac cctctcgcca catactcagc gtaactatcc
    37381 ggagctacaa aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct
    37441 gaccgtctgc gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg atttctggaa
    37501 ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca tcgcactcat
    37561 tcttttcttc tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc
    37621 ttcggatatt catggccgcc cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt
    37681 ccgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat
    37741 cggcgtcaat atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt
    37801 tggttcgcaa acgctggtaa aagatattat caccggggta tttattcagt ttgaaaacgg
    37861 catgaacact ggagatttgg tgactatcgg gccgttgacc ggcacagtgg aacggatgtc
    37921 gattcgctcc gtgggcgtgc gccaggatac cggggcgtat cacatcattc cgtggtcttc
    37981 gataaccacc tttgctaact tcgtccgcgg tattggttcg gtagtggcaa actacgatgt
    38041 tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg taacggaatt
    38101 gatggaaaac gaagaaattc gcgggctgat tattggtgaa ccgaactttg ccgggattgt
    38161 cggcttaagc aataccgcgt ttacactgcg tgtttcgttc accacgctgc cactcaaaca
    38221 gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt
    38281 tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc
    38341 gctgccgccg ggggagccaa cgctttaacg ttggcgatta acaaaacggc ggcgctgctc
    38401 gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt
    38461 ttttaccacc ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg gtaagttttc
    38521 accacgagat accagcgagg taaaccacat cacctgcttc gcgaagcctt tgctctcttc
    38581 aatcatcttt ttgataaagt tgacttcacc gccttcacac cacaactcct gttgctgacc
    38641 accaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc
    38701 tgcccgcgcc gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg
    38761 ctcgtttttg tggatgatgc cgttaaaaat cgccccactc tctttttgcc gacgcagacg
    38821 aatggcgcgg ttaagccccg gattagcact gataatcgcc tgcgcactgc ttaacgcctg
    38881 gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata
    38941 aatacagttc gcaccaacgc cgatatccag aatgctggca ttcgctggaa ttgttccgct
    39001 tgcttcagcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggtgg
    39061 gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc
    39121 cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct cccctgcggg
    39181 tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta
    39241 gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc
    39301 ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt
    39361 atcattcccc aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca
    39421 ttatgcatgt cattgatttg tcttattttg taaaaatata tcgttcaaaa aataagcatt
    39481 tctctgtcaa ttttttgcgt catttgcatc gcgcaggcga cctacactta tcaggcacta
    39541 cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg
    39601 tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg
    39661 gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg
    39721 aggataaact ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg
    39781 caggcggaaa tgaccagatg ttaggtactg caaccatcta caaataaccc caccaacgta
    39841 actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatgtg
    39901 aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcatcaa
    39961 gtgataatgc ttatcaaaat tattatcact tgcacgaaca ctatcacg
//
LOCUS       DAJVPT010000025        39999 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVPT010000025 DAJVPT010000000
VERSION     DAJVPT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(86..1339)
                     /gene="amiC"
                     /locus_tag="OQM47_001291"
     CDS             complement(86..1339)
                     /gene="amiC"
                     /locus_tag="OQM47_001291"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1479512.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1571..2902
                     /gene="argA"
                     /locus_tag="OQM47_001292"
     CDS             1571..2902
                     /gene="argA"
                     /locus_tag="OQM47_001292"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1479513.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2964..4790)
                     /gene="recD"
                     /locus_tag="OQM47_001293"
     CDS             complement(2964..4790)
                     /gene="recD"
                     /locus_tag="OQM47_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1479514.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4790..8332)
                     /gene="recB"
                     /locus_tag="OQM47_001294"
     CDS             complement(4790..8332)
                     /gene="recB"
                     /locus_tag="OQM47_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1479515.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8325..11213)
                     /gene="ptrA"
                     /locus_tag="OQM47_001295"
     CDS             complement(8325..11213)
                     /gene="ptrA"
                     /locus_tag="OQM47_001295"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1479516.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11389..14757)
                     /gene="recC"
                     /locus_tag="OQM47_001296"
     CDS             complement(11389..14757)
                     /gene="recC"
                     /locus_tag="OQM47_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1479517.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14770..15120)
                     /locus_tag="OQM47_001297"
     CDS             complement(14770..15120)
                     /locus_tag="OQM47_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1479518.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15105..15512)
                     /locus_tag="OQM47_001298"
     CDS             complement(15105..15512)
                     /locus_tag="OQM47_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1479519.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15509..16072)
                     /locus_tag="OQM47_001299"
     CDS             complement(15509..16072)
                     /locus_tag="OQM47_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1479520.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16063..16533)
                     /locus_tag="OQM47_001300"
                     /pseudo
     CDS             complement(16063..16533)
                     /locus_tag="OQM47_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16717..17511)
                     /gene="thyA"
                     /locus_tag="OQM47_001301"
     CDS             complement(16717..17511)
                     /gene="thyA"
                     /locus_tag="OQM47_001301"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1479521.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17518..18393)
                     /gene="lgt"
                     /locus_tag="OQM47_001302"
     CDS             complement(17518..18393)
                     /gene="lgt"
                     /locus_tag="OQM47_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1479522.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18544..20790)
                     /gene="ptsP"
                     /locus_tag="OQM47_001303"
     CDS             complement(18544..20790)
                     /gene="ptsP"
                     /locus_tag="OQM47_001303"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1479523.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20803..21333)
                     /gene="rppH"
                     /locus_tag="OQM47_001304"
     CDS             complement(20803..21333)
                     /gene="rppH"
                     /locus_tag="OQM47_001304"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1479524.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22018..22707
                     /gene="mutH"
                     /locus_tag="OQM47_001305"
     CDS             22018..22707
                     /gene="mutH"
                     /locus_tag="OQM47_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1479525.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22776..23489
                     /locus_tag="OQM47_001306"
     CDS             22776..23489
                     /locus_tag="OQM47_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1479526.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23627..23845
                     /gene="ygdR"
                     /locus_tag="OQM47_001307"
     CDS             23627..23845
                     /gene="ygdR"
                     /locus_tag="OQM47_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1479527.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23953..24993
                     /locus_tag="OQM47_001308"
     CDS             23953..24993
                     /locus_tag="OQM47_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1479528.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25026..26216)
                     /gene="lplT"
                     /locus_tag="OQM47_001309"
     CDS             complement(25026..26216)
                     /gene="lplT"
                     /locus_tag="OQM47_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1479529.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26209..28368)
                     /gene="aas"
                     /locus_tag="OQM47_001310"
     CDS             complement(26209..28368)
                     /gene="aas"
                     /locus_tag="OQM47_001310"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1479530.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28954..29985
                     /gene="galR"
                     /locus_tag="OQM47_001311"
     CDS             28954..29985
                     /gene="galR"
                     /locus_tag="OQM47_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1479531.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29992..31254)
                     /gene="lysA"
                     /locus_tag="OQM47_001312"
     CDS             complement(29992..31254)
                     /gene="lysA"
                     /locus_tag="OQM47_001312"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1479532.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31376..32311
                     /gene="lysR"
                     /locus_tag="OQM47_001313"
     CDS             31376..32311
                     /gene="lysR"
                     /locus_tag="OQM47_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1479533.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32298..32990)
                     /gene="ygeA"
                     /locus_tag="OQM47_001314"
     CDS             complement(32298..32990)
                     /gene="ygeA"
                     /locus_tag="OQM47_001314"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1479534.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33119..34537)
                     /gene="araE"
                     /locus_tag="OQM47_001315"
     CDS             complement(33119..34537)
                     /gene="araE"
                     /locus_tag="OQM47_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1479535.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34852..35613)
                     /gene="kduD"
                     /locus_tag="OQM47_001316"
     CDS             complement(34852..35613)
                     /gene="kduD"
                     /locus_tag="OQM47_001316"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1479536.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35643..36479)
                     /gene="kduI"
                     /locus_tag="OQM47_001317"
     CDS             complement(35643..36479)
                     /gene="kduI"
                     /locus_tag="OQM47_001317"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1479537.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36766..37947)
                     /locus_tag="OQM47_001318"
     CDS             complement(36766..37947)
                     /locus_tag="OQM47_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1479538.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38202..39431
                     /locus_tag="OQM47_001319"
                     /pseudo
     CDS             38202..39431
                     /locus_tag="OQM47_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         9926 a        10820 c         9702 g         9551 t
ORIGIN      
        1 ccccgcaacc aattattgaa caccctaacg ggtgtttttt tgtttctgat ctcccataaa
       61 aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc cagcgttgcc ccatcggcaa
      121 aatacgcttt aatccccgca agaatagact ccgcaacttc ctgctggaaa gtcgccgttt
      181 tcagtttacg ctcttcctca acgttactga taaacgccgt ttcgaccaga atggagggaa
      241 tatctggtgc ctttagtacg gcaaacccgg cctgttcaac ttgattttta tgcagcttgt
      301 tgatttttcc gagcttattc agtaccgctt taccaaactt aaggctgtca gcaatagtca
      361 gcgactgcac catatcgaac atggtgtggt cgacatagcg gtcaccgctt ttgctcacgc
      421 caccaatcaa gtccgaggcg ttctgggttt gtgccagata ttttgccgca gtactggttg
      481 cgcctttggt tgagagcgca aacaccgagg aaccgctcgg ctgacgactg gtaaaggcgt
      541 cggcatggat agagacaaac aggtcagcac gctgtttctg ggcttttgct acgcgcactt
      601 gcaacggaat gaagatgtct tcattgcgcg tcatgtacac cttcatattg ccctcttcct
      661 cgatcagaga gcgcaaacgg cgagctattt gcaataccac atctttttcg cgcgttttgt
      721 atttccccac cgcaccggag tcttcgccac cgtggccagg gtcaagcata atgacaatcg
      781 gacgatcgcg ccctgcttta cccggttgtg gaccactttg cgctggcggc acctgctttt
      841 cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag cgggtcctgc atatcctgtg
      901 cattggccgg atagaggtcc atcaccagac gctctttaaa cccggcgacc ggcgcaaggg
      961 caaacagctg cggttttacg ttttgcttta attcaaaaac catgcgtacg gtttgcgggt
     1021 caaattgccc gacgcgcgcc gacttgatga acgggtcgtc tgcacgaatt tgtgccgcca
     1081 tccccttgag taccgagttc aggtttacat cttcgatatc caccaccacg cgttcagggt
     1141 tactcaacgc gaactgctta tatttcagct gacgatttga ttctaccgtc acgcgggtgt
     1201 agctggacgc aggccagacg cgcaccgcca cgacctggct aaccgcagcc aggctgacct
     1261 gacttacgct caatagccac atagcgcccg cgccttgcag taaacgacga cggctgattg
     1321 cagtgttgga tcctgacatg cctctcccga gcaaaagaaa tctaagctgt gtaacaagta
     1381 aacgactaat ttgaccggaa actttagcga aagacgcata atctgtcatc taataaacgg
     1441 taaacattct ttttatattc acggcattac tgataaaaaa gtcgctctcg cataaaattt
     1501 acacttgcac cctgcgaaaa aacagaataa aaatacaata atttcaaata atcatgcaaa
     1561 ggggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc gagggattcc gccattcggt
     1621 tccctatatc aatacccacc ggggaaaaac gtttgtcatc atgctcggcg gtgaagccat
     1681 tgagcatgag aatttctcca gtatcgttaa tgatatcggg ttgttgcaca gcctcggcat
     1741 ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg ctgcgcatca
     1801 ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac gccaaaacac tggaactggt
     1861 gaagcaggct gcgggaacat tgcaactgga tattactgct cgcctgtcaa tgagtctcaa
     1921 taacacgccg ctgcagggcg cgcatatcaa cgtcgttagt ggcaatttta ttattgccca
     1981 gccgctgggc gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc ggcggattga
     2041 tgaagacgcg ctccatcgtc aactggaaag cggtgcaata gtgctaatgg ggccggtcgc
     2101 tgtttcagtc actggcgaga gctttaatct gacctcggaa gagattgcca ctcaactagc
     2161 catcaaactg aaagctgaaa agatgattgg tttttgctct tcccagggcg ttactaatga
     2221 cgacggtgat attgtctccg aacttttccc taacgaagcg caagcacggg tagaagccca
     2281 ggaagagaaa ggcgattaca actccggtac ggtgcgcttt ttgcgtggcg cagtgaaagc
     2341 ctgccgcagc ggcgtgcgcc gctgtcattt aatcagttat caggaagatg gcgcgctgtt
     2401 gcaagagttg ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa gcgccgagca
     2461 gattcgtcgc gcaacaatca acgatattgg cggtattctg gagttgattc gcccgctgga
     2521 gcagcaaggt attctggtgc gccgttctcg cgagcagctg gagatggaaa tcgacaaatt
     2581 caccattatt cagcgcgata acacgactat tgcctgcgcc gcgctctatc cgttcccgga
     2641 agagaagatt ggggaaatgg cctgtgtggc agttcacccg gattaccgca gttcatcacg
     2701 gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag cagagcggct taagcaaatt
     2761 gtttgtgctg accacgcgca gtattcactg gttccaggaa cgtggattta ccccggtgga
     2821 tattgattta ctgcccgaga gcaaaaagca gttgtacaac taccagcgta aatccaaagt
     2881 gttgatggcg gatttagggt aagcgatgaa aatcaccgga tgcggcaggc gtaacactcg
     2941 tgcgtcgcat ccggcaatta cgtttattcc cgcgaactaa acaacgccgc cagaccactg
     3001 cgccgctcag tacgagtggc gattgccgca cttaatatac gctcatcggc atacagcgac
     3061 agacgacgac gcgcgcgggt caccgcggtg tataccagct ctcgcgttac taccggcgtg
     3121 cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg atccctgcga tttatgtacc
     3181 gtcatcgccc acgtagtttc gtgctctggc aagcgactcg gttgcacaga cttaatattg
     3241 ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc gatccagcgc aataccgata
     3301 tcgccattaa acaacccaag cgcgctgtca ttacgggcaa tcatcaccgg tcggccttcg
     3361 taccaacgag agtgcggatg acgatgaatt ttgcgcttct gttgcataaa ctgttcaatt
     3421 cgctcattca gtccagccac gccaaacggc ccttcccgca gggcgcacaa aagctggtac
     3481 tcattgaacg cctgaatgat taaatccggc tcggcacgcg cttgcagcag atccagataa
     3541 cgtccgtaac ccgcaagagc ttcctcaagc atcgcaatat aatcttcgcc gctctgtaaa
     3601 agtcgttttt cgatatcagt aaaatcctgc tgaaaaacgg ttttcactgc cgttttatca
     3661 ccacggttga tcgccgcagc taactgacca atgccagaat cgctgccgaa acgatagctt
     3721 ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt ctgtgccggt tcctgccgga
     3781 acgtgagtcc ccgtcaggcg gcttagctgc ctggcacgct cggcggtaaa gcccgcgttg
     3841 gcataagcgc agatatcgcc cagcacagcc ccagcctcaa ccgaggccag ttgatcacga
     3901 tcgccgagaa agatcactcg cgcatgatct ggcaaggcgt cgatcagtct cgacatcata
     3961 ggcagatcga tcattgacgc ttcatctacc accagcacat caagatgcag cgggttaccg
     4021 gcatgatgac gtaaacgctg gctacccggc tgcgcgccca gcaatcggtg caaagtgctg
     4081 gcatcttccg gaatgcgttt cttttgttca tcggtcagcg gtaactgtcg caaagccttg
     4141 ccgagagatt cggttaagcg cgcggcagct ttacccgttg gtgcagccag acggatacgg
     4201 cagcgttcgc cgtcggccat ttgaattaac gctgccagca acttcgccac ggtggtcgtt
     4261 ttaccggtgc ctgggccgcc ggaaatcacc gagatccgcc gcgtcagcgc cactgccgct
     4321 gcaacttttt gccagttaat ttcatcgctt actggaaaaa gtttgtccag ggtttgcgcc
     4381 agtagagctt catcaacctc aatggtatga ttcacctcat tgaagaagcg cgctaccgtc
     4441 cgctcgttac accacatgcg attcaaataa agacgatcgc cacagaggat catcggcgtg
     4501 ggttcatctc ccctgctgac cgcttgagaa gccagcaagc attcttccca attttgtagc
     4561 tcaccgattt cactgacaca ggtcgccaac agcggattcg acgcctcgtt attttccagt
     4621 cgtgaaagcg gcaaacagac gtgtccctct ccggcatcat gacttaacag tgccgccgcg
     4681 agggtgacgg caggatgttc atctcccgcc acggtcaggg caaattgcac atccagcggg
     4741 cgtagctgtt tgtgctccac agcttccagt aattgctttt gcaatttcat tacgcctcct
     4801 ccagggtcat accggcaaac atctcatcca tcagggcaat caacccggcg ttgggtcggg
     4861 ttgtgtaaat cccttgttgc ggatgttctt tatcaacgcc acgcaggaac agatagataa
     4921 cgccgccaaa gtgaagatca tagtcgtaat cagcaatgcg atggcgcaga taacgatgca
     4981 gcgccagggt ataaagctga tattgcagat catagcggtg tgcctgcatc gctgctgcca
     5041 tagcctgttg ggtgtaagcc gaactgtctt cacccagcca gttggattta tagtcgagca
     5101 ggtaataacg cccttcgtgg cggaatacca ggtcgataaa gccttttaac atgccgcgta
     5161 cctgcatgaa ctccagcggc gggcagccag cggatagcgg gtcaaactgg cgaattagtg
     5221 catcaagctg gctggcgatg agcggttcgc taatcggcag gtaaaactcc atctccacct
     5281 gtttatcgcg gtcggaaagc tgactcaggc taacacccgt ttcattgaga ggtgcctgga
     5341 ggacagccgt gatccactcg gtcaacaccg gttcccactg cgattcaaag ccgccgagtt
     5401 ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt aaaatcgagg tcttcaaaca
     5461 aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa ctgatgcggt gttaacgtcg
     5521 gttcttcaac gacgctgacc acgcctgcgg catcaacatc cagccgtggc atcagatcct
     5581 gagcgatacc gtgaccacgc tgctgcaagc cggagtagct ggtgacacgc cagttatcgc
     5641 cgggcaatcg ttgtagtgtc ctcgcattca gttctgcagt taaagcatca ttaacctgcc
     5701 agggctggtt atcaccagtt tgtgccgttt gccaggcaat atcatcatcg cataacgctt
     5761 caatacaggt gcgaagccct gccgcatctt gcggttcccc tttttgcagc aaacgcccga
     5821 gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc acgacggcgc accagcggtg
     5881 caacgccgag actgcaatgc caaaccgaac gcgtcagcgc cacgtaaagc aaacgcagat
     5941 cttccgccag acgttcgacc tccgcgaggt cgacgctttc tggcgcagca ttaagatcca
     6001 aaactgcctc aaacgagtgg cgatcgtgat aaaacgcctg atcctggacg cggaaattgg
     6061 taataaatgg caaccagacc agcggatatt ccagcccttt cgatttatgg atcgtgacaa
     6121 tctgcaccag atgtttatca ctttcgaggc gcatttgttg gctggaggca ttactgtctg
     6181 gctcgaggat atgttgcgat aaccagcgta ccagcgcatg ttcactttcc agctgcgttc
     6241 cggcttcttg aagcagttcg ctgatatgca agatatcggt aagacgccgc tcaccgcctg
     6301 ccgttgccag caagttttct gcaatgttac gcgccgacat cagcgcccgc agcatcggca
     6361 taacgccacg tttgcgccag atttgccgat aaccatcgaa ctcttcgact accgcatccc
     6421 acgcatgttc gtcattattc agcgtttcaa tatccagcgc attcagcccc atcattgacg
     6481 ttgccagcgc actgcgcagg gtgttctcac gttcgggcgt catcaccgcc tgcaacaacc
     6541 aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc gcggttcgaa aggtaaacgg
     6601 aagggatttc cagcaacgtt aaggcatcgc gcacctgggc ggcctcctgg cggctgcgca
     6661 ccagcacgct gatgtccgaa gcacgcaccg gacgcgcgtc gtcgccgttc atcagcaacg
     6721 cttcgccccg ctgtccggct tgtagccagt cgcggatttg cgcagcacat acctgcgcca
     6781 tggtactttg atagtcgcca acgccgcagc tttcgccttc catcagccac attttcatcg
     6841 caggctgcgt ttcaccttta aatacaaaac gtaacgcctg atttttcccg gctgatttca
     6901 ctggaataaa cggtatttcg cgaaacatga acgtgtcatc agtctggctg aaaagcttgt
     6961 tcacactgtt caccattcca ggtgcggagc gccagttggt gtctaaagtg tagtgggcat
     7021 gaacttcact acgcgccttc atataagtga agatatccgc accccggaat gcatatatgg
     7081 cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg ctgatggtgc cagatacggc
     7141 gaaaaattcg gtactgctgg gggtcggtat cctgaaattc atcgatcatt gccaccggga
     7201 atcgcgtacg gatcgccgct gccaacacct cgccgctttc gctacgcagc gcggaatcga
     7261 gccgacttaa catgtcatca aaacccaatt cgccacggcg gcgtttttca cgcgctactg
     7321 tttcgcggat ctcagccaat gcgcgggtga tcaacagatc gcggatcgac aatggttctg
     7381 caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg ggtttccccc ccggccttcg
     7441 tgcgatcttc taagaaacgc tgggagaatt tttccagcga ctccggcaac tgataactgt
     7501 ttgtctcttc ttctgcccag gcgctgatct tttcgatcca tttagcctga ttgctacggt
     7561 taaacttgcg tcgatcaata ccagaagatt cgatcagcgc atccagttca cccactgcgt
     7621 cgcgccactg ctgttttacc gtatcaatac gcgccacaat ttgcgcgtgg cgggaagcca
     7681 gcgtttcatc atcgggcggc ggtgctttga taaccggcgg ttcgccttgc agataacgat
     7741 taatatcgcg cagcaacgcc tgcggccctt tccaggtttc aaagacgacc tgggctattt
     7801 cacgcggcag cgggtagcag tggcgacgcc agaaatcggc gcaggcctgg tagcgtagca
     7861 gagactcatc ttcaatcagc tgctgctcaa acagcatgcc ggattcaaag gcattcaggt
     7921 tgagcatgcg ctggcaaaag ccgtgaatag taaagactgc cgcttcatcc atctgccgtt
     7981 cggctaacaa caaccactgc gcggcttgcg ctttatcgtc gatctcttcc agcaggcgtt
     8041 cgtacagtgg attgtcggtg gtttcacgca gacaggcgat gcgcaactcg tggatattgc
     8101 tacggatacg accgcgcaat tctgccgtgg cagcctcggt aaaggtgacc accagcagtt
     8161 cttcaacgat cagcgggcgg ggaaaggcgg cggaaccgcc tagtccaagt aacaggcgca
     8221 aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc agaggcttca atcaggcgct
     8281 cgccctgtaa gggcaagcgc aaaggatcta gtgtctcggc gacatcactc attcttttca
     8341 ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct cccacacttt ccagccttca
     8401 ggatgcacat attcggcttt cccgttctgg ctgccggaaa tctgcgacag aatagccatg
     8461 ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa gtttttgcgg cgtcagcagt
     8521 tttatctggg ccacgatttt atcacgcgaa tcgaagcgca tattgccgcg atcgaaatct
     8581 ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg cctgcagcat ctgggtaatt
     8641 accgcctgct ggatttgcgc aaactcatct ggcttcatcg ctcgcaattt tgcctctgcg
     8701 gttgggaaaa acgccttgta acgctcccac aagaatgaag gctgtttatc attgctttgc
     8761 aaaaggaagc ccatgcccca ctgacgcccc acgctcattg gaaacgcaaa cacggcatag
     8821 cccaattgtt cttcggtacg caactgattg tagaaccacg gctgtacgat ctgccccaac
     8881 agagagctat aggctgagct ggtgtattca tcgtagccag tcggtacaaa taccgctgcc
     8941 agtgcggagt cggtgctgtt accggctttt tcaaagatga cggattgttt tttatcgacc
     9001 actacatctt tgtttcgaca ccactctgaa ccatcagcgc ccaactgttt ttgcacatcg
     9061 cgtgccagcg ttgttgcctg ggcctcggtc atgttgccga taaccataaa ctctggtcga
     9121 gcccctgatt ttaaggcgtc gcgataggcc agcacctctt tcaacgtaat ggagggcaaa
     9181 attttacgcc gttcatctcg cgagaagtac ggcacttgcg agagcatctg cgcgggcata
     9241 atcgcctgct caaacgcttt acccttttct gcggaatcca tcatctggtt ataccaggac
     9301 ttcgcctgct caagctgctc ttccgtagcg gtatagctaa agtacccctc gagcaatgcc
     9361 tggaacagct gcggcagacg ctgggtgtaa ccattagcat taatcataag gccgttgtta
     9421 gcgttggtgg aaaaacttat gccaccaacc gacgcctggt tgcttaactg atcaagcgcc
     9481 agccctgcga gataatcatt gagcgcaaac atcacctgat tgcgggcgct gtccatggct
     9541 ttcggattac gcaaaatcag gctgacatca gctttgggct cgctggcaaa ataacggctt
     9601 ggcgcataca ccacgcgcag attcgactca tcaacaatca gctctggatg gtcgtatttc
     9661 ttctctgact taatcagcga gaaatcatca ggaatataag ggttaagctc tggcaaagag
     9721 agcgcaatgt cggcggcttt tttctgccag tcggcgaaag tttgtgcgct gattttatcg
     9781 acctgatacg gcgcatcgac aaagtaagcc gttttgttgt gcggctcttt cgggctgata
     9841 taccagatac gcgcattctg cggcgtcatc atcgccagac gttcctttac tgctttagca
     9901 tcgtaccgat cggcaatatt gactgcatcc agcgtatgct caacaggaac gcgaatcatg
     9961 gtatctgcca gccattcgac gtaatccata tcacgggtga tcgacggata acggaagtcg
    10021 atatccagca cattcgccag ttcatcgaag tattgtttat caatgccttt ttcacgtaac
    10081 agattgagat agctaaaaat tgccgccaca acctgatcgc gattagccag gcctttatca
    10141 gttaaagacg cagagatcgc taatacgccg ctgttgccgt tgacgatagg atcggagttg
    10201 gcgctaatgc cctcaactaa tccctgcttt tgcagccagt cagaaagtgt acctgggctg
    10261 cgattgccaa tcagataggt aatcaattca tcggttttac tacggaactt cgctgagttg
    10321 ttatcgatgc gaaactcaac gcgcaacact ttacgcggca gcgcagggac gtaatgaatg
    10381 ataatgccct tttgcgcgtc ggtgactacc ggcacggtga tttccggttt tttgctctct
    10441 ttgttcggca cgcgaccaaa ggtgtccgcc gccatttttg ccaactccgg cagcggttta
    10501 ttactgtaaa taaccgcctt catcaaattg gcggaatagt acttctcgtg gaaatctttc
    10561 agcgcctgct gcaccggatt accaggtttg tcgcttaaag tttcgaggtt accaccagaa
    10621 aactttgaac cggggtgtgc cgggttaatg gtttctgcgc tgacctgtgc catgcgcatc
    10681 ccgtcacgcg tacgcgccat ggttaattca gcgttcactg cattacgctc acgttcggcg
    10741 tatttcttgt cgagcaaggg ttctgcaata gcatcggcca ggcggtctac cgcaccgggc
    10801 aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat acggcgcagt gctggcattg
    10861 tgactaccgc cgtgcatttt gagatattcg gccagactgt cagcctgcgg gtactttttc
    10921 gaccccatca gactcatatg ttcaaggtaa tgtgccagcc cctggtacgc ctcgggatct
    10981 tccagcgacc caacgggcac taccagcgcc gagagcgatt taactgcctg cggatcagaa
    11041 accagcaaga ccaccatacc gttatccaga cgtatagcct gatactggcg gttatcttta
    11101 tcacttttac ggatggtttc ctgaatcggc tgccatcccg tttctgcctg acttaatggt
    11161 gcccagaggg caacaaacaa caataatgct ttgaaccagg tgctgcgggg cattcacgga
    11221 cctcataatc aactttattt tctggacaga ttcaacaggt taacgcaaat catctgccag
    11281 aatttaatct tgtgctgcac gagtcagcct atgtttatat aaccatcagt ccgtgactgg
    11341 tgcgcatcat aaagtaagca gatagattgc gcaattttta tacaacactc atgactgatt
    11401 aaagcgaaac agcggtaaca ggaaacgttg cgactgttca acgatggcct ccattgtctc
    11461 tggtgttaat tgccgccaga gccgttgata ccagatatca tcaccttcgc cacgcaccat
    11521 catgttgcct tcgtaagcct gaaggaattt cgtacgggct ttttgcaacg tggaatcgtc
    11581 atccagcatg gcatcgtttt gcgcgtcata acaggttttt agccacgcgc cgccactttc
    11641 aggtaacacc agcaatggcg cggacattcc ttcacgatac ccctcaatca gttgtgagag
    11701 gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc cactcaccgt ctttgcgtag
    11761 aaaaaggcga ctttcgccat taccaccgct ggcacagtag acaaggtgtt ccagccaaag
    11821 ttgcattccc tgcgccacac ttaataaaga gggacgccag cgcaacaggc catccggctg
    11881 cacctgcggc aaccagccag ttatctgcac accgttgcag gcgagatcaa tttccatact
    11941 ctgccccggc tggcgacagg caatgactct gtcggcaagc tgctgcatct cctggcactg
    12001 tgtttcccag aaaatttcac caaaagcgcc atacggtaaa tcccctgccg cccggaagcg
    12061 gcggaacaag cgttcggcat catcctgctc aaccagtgca ttcaataact gctgattgat
    12121 ttgataacgg ctaagtcctt ccagaataaa tggctcggtg tctgggattt cgctgtcttc
    12181 agtatggaag ttcacctgca aacgcatctg gaagaacgcc cgcaccggat gtgcccagaa
    12241 tcgttgtagc gtttccagcg gcacggtttc cggtaaggta aacggcagcg gctgaacaaa
    12301 ttcagaatgt gctttaccag cctggctggc cgcaggtagc cattcacgag catagctttg
    12361 tcgttcgcct ggctggtagt tttgcggatc aaacggcatc cgggtatgga ggcaagtaag
    12421 atgcgctttt acccttgcct cgctttcatc acagttgagc gcttcatcgc ccggtagata
    12481 atgactttgc ccgatgtagt cgatcagttc ctgcaccagt accgacggga aacgttcact
    12541 gttatcctga atggaacgcc cgatatagct gatatagagt ttttgctgcg cggaaattaa
    12601 cgcttccagg aacaggtagc ggtcgtcatc gcgacggcta cggtcgccac gcttcggttt
    12661 ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt ggataaacgc cgtcgttcat
    12721 tcccagcagg caaaccactt tgaacggaat tgaacgcatt ggcatcagag tacaaatgtt
    12781 aaccggtccg gcgagaaaac gctggctgat acgttcttga tccagacgct gtgccagttc
    12841 atcacgcaat agtgacagcg gcaccgcgtc gccatactgc gcacctaaac cttcggcgat
    12901 aatcgcctgc cattgttgtt cgatcagcgt catcgccgct tcggtttccg catccggcag
    12961 gaagaaggcg ttgagcatat cgcgacaaac cggcaaccac tcttctagcg gacgctcctg
    13021 tgccagcccg cgacgccaga tgtttagctg cattagcagt gaagccagat gccccaccag
    13081 ttctgcaatt aagccgctcg attcatcata aggtagaacc gattgccact cgccctgcgc
    13141 gctctccatc gcgtagccca gcaacatacg cgtcaggcca aatcgccagg tgtgttgtcc
    13201 ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct atcccccaac gaatgccgga
    13261 ttcgttgacc cactggcgta aataacgcag cccttcttcg gtgatgtcaa accgcgccgc
    13321 cagcaccggc acatccagca gcgccagcac atcctctgac acaaagcgac tgtcaggcag
    13381 tgataacagg ctgataaacg cttccagtac cggatgtgac tgccgcgcac gacggtcgga
    13441 aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca aacactgcct gaataaacgg
    13501 actgtagctg tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa gtgtcggggc
    13561 ttcctccagc atcgccagca agcgatcgtg taaaacttca acttcacgct gcgggctatg
    13621 gcaaacgtgg aatgtgatac tgctatccag tggatcaagc gggcgtttgt tatcgctacg
    13681 ggaaaactct tcgatgttca cacccgcaac ggcgcggttt tccagttcca gaatgtcaga
    13741 ctgaatgtta tgcagcaggt tatctggcgt cacatcgaca aaagcgtcca gctcctggct
    13801 gctctccagg tcagaaagga gataaatgta gtcgcgccca agcttccccc atgaggccag
    13861 cagcgagttg ccgacatcct gttcaccatc gctgttaaag agctgtccgg cattttcgct
    13921 atcgcgaaat agcggcaatt cgcgatcttc aaaactgtgt cgacgctgac gagtcagcag
    13981 tttcgccaga taagcaggat ctttaatatc gccccagtaa taacggcagg ggttggtaaa
    14041 caggagatgg atttcaatat gtttacccag cgcctggagc gcctggagat aaacaggcgg
    14101 taacgcggaa ataccgcata taaagacgcg cgaaggtaac cccggcgggc aggtcgtcgc
    14161 ggactccagc gtttcgataa agcgctgata gagattcgcg cggtgccagc gcggttgtcc
    14221 gagttcatgg gtatattcca ccagcgcctt ccacaacggg gcttgccagg cctgtgcttc
    14281 tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc agccagtccg gacgatagac
    14341 cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc tggaacagtt ttcgcttgtc
    14401 gctatcgtca gtcagataat gccgcaacag ggtaaagtct tcgcgctcca gcaattgcgg
    14461 cagcagagtc atcagtttcc agctcatgct ctgtttgtta aaggcgctct ctttggggat
    14521 ttccggtaac acccggacga acatatccca gataaagctc gctggcagcg gaaaatcaat
    14581 gtttgccgca ataccaaact tttgcgacag ggtcatttgc agccactgcg ccataccggt
    14641 actttgcacc agaatcatct ctggttcgaa aggatcgtcc agccgttcgc gttcgacaat
    14701 aaactccatc aacgcttcca gcacgtccag acgattggaa tggtagaccc ttaacatagc
    14761 ggctcctgac tactgacaat tcgggcaatg caggcgggtc atctcgcctt ctctgccccc
    14821 gggtgaaact agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct gcattcggtt
    14881 gacctgccag ttggcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc aaccatgccg
    14941 ccagagcttt tggtactggt ttctgctggc gagactgttc attgatgttc gctggatacc
    15001 cgataacgca gtgacaatca tcaccatcag caccatcagc accatcagca ccatcagcac
    15061 catcgcaacc attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat
    15121 aatgccccct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaattttt
    15181 ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aatcaataag
    15241 gcttcattat ctgccagtaa acgcaaacaa acctgggcat cggttccagc gtactgcgag
    15301 cactgaactg ctggctgcgt ctgccaggag tgcatttttc cccacgccag cgccgactga
    15361 acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga
    15421 tcctgctgac tcattccttg caatagcaag ctacccaaaa ccagcagcat caggaccaga
    15481 gccagtgacg aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg
    15541 catccaccac ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg
    15601 gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg
    15661 gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca
    15721 gcacatgctc cttcagacga aatccaatct ggtcggactc ttttaccggt tcgcgatccc
    15781 agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt
    15841 ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga
    15901 cggtaaatac ccgcagccag atttcatctt ccagtgccag cttacgagtg ctcgttaaac
    15961 tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg
    16021 ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt
    16081 ttctccttgc tgataaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc
    16141 cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc
    16201 aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg
    16261 tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc
    16321 ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata
    16381 gagcaaatag tcccgcgcct agctggcggt ttgccaaagc cgttgcgact gctgccagta
    16441 ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt
    16501 ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa
    16561 aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag
    16621 gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac
    16681 gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaac
    16741 gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga
    16801 ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag
    16861 atgagtttga tccatatggt tgctgtacag gtgcgtatcg ccaccggtcc agacaaaatc
    16921 acccacttcc agatcgcact gctgcgccat catatgcacc agtaacgcgt agctggcaat
    16981 gttgaacggc aggccgagga agacgtcaca ggagcgctga taaagctggc aagagagttt
    17041 gccgtctgcc acatagaact ggaagaatgc atggcacggt gccagcgcca ttttatccag
    17101 ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg
    17161 gttcagtacc gtagtaatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca
    17221 ctgtttacca tacactggcc cgaggtcgcc attttcatcg gcccattcgt cccagatggt
    17281 gacattgttt tcgtgtagat aagcaatgtt agtatcgccc tgcaggaacc acagcagttc
    17341 gtggatgatg gaacgcaggt ggcaacgttt ggttgtcacc agcgggaatc catcctgcag
    17401 gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt
    17461 ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca
    17521 ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac
    17581 aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa
    17641 ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag
    17701 gaacaagcct gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat
    17761 aaacagcacc acaccttcca gcagcagttc gtaaagctgt gatgggtggc gcggcagcac
    17821 accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc
    17881 tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa
    17941 ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc
    18001 gataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga taatcatcac
    18061 cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag
    18121 atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc
    18181 cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca
    18241 gccactgccc ggacgattcg cccgacgtgt tgccagccac attgcaaaaa tgaaccccac
    18301 cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc
    18361 aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg
    18421 ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa
    18481 taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc
    18541 gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac
    18601 ctgatgacga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc
    18661 ttcggcaaaa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat
    18721 agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat
    18781 ttcaccgcac aaacgaagat cgataccatg tatttccgct tcccgggcga tcatcgccag
    18841 agctcgtaac attgcaggat gaagactgtc gtaaatgttc gccacccggg tattgttgcg
    18901 atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgaccct
    18961 ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg
    19021 tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag
    19081 gcggcgtgct tcgtcaactt catcgaggct ggtgaccatc ggcaacagaa tattcaggtt
    19141 gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg
    19201 atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat
    19261 gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt
    19321 gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact
    19381 ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac agctgcccag
    19441 tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct
    19501 ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc
    19561 actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc
    19621 gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc
    19681 catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt tggctgcgcc
    19741 atctcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga
    19801 cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc
    19861 atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcaccagccc gctctttgag
    19921 atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc
    19981 ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc
    20041 cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc
    20101 aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg
    20161 ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa
    20221 cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat
    20281 tcgcgtctgg cgatattgcc caaacagggc agtcaactgc gactgagaaa gaatagctgc
    20341 catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc
    20401 tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa
    20461 aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg
    20521 cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc
    20581 aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta
    20641 gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag
    20701 acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt
    20761 ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg
    20821 ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc
    20881 gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg
    20941 ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt
    21001 gatttctgca tcgccgctta ccagctgcaa gagaaaccat ttttgttttt ggccgataca
    21061 aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg
    21121 cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg
    21181 gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga
    21241 gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc
    21301 tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata
    21361 tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac
    21421 actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg
    21481 atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac
    21541 gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta
    21601 gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac
    21661 ctctcattta cgggttggcg aaagatcaac caatgccgtg tttatccaca gaatgtgcca
    21721 ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg
    21781 aaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag
    21841 atcctgttta ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat
    21901 taccggcagc ctaaaaagca atataaatca gagaattgaa caacacatgt ggaaaaagtt
    21961 acactgcgaa tattcggcac ataattgctg tttgtttttt aaccaaggta tcatgacatg
    22021 tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca
    22081 cagcaacttt ctggttatac attgggagaa ctggcggcac ttgacgggct ggttacgcca
    22141 gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc
    22201 agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc
    22261 cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc ccggttaacg
    22321 ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg
    22381 tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg
    22441 ttgctgtgga gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta
    22501 atggatatga ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta
    22561 cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc
    22621 gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta
    22681 ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat
    22741 aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc
    22801 ctaacgcttg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca
    22861 atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc
    22921 gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatctctt
    22981 gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg
    23041 atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg
    23101 aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta
    23161 tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt accgctgtgg
    23221 gtctgtcaga tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga
    23281 tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg
    23341 cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg
    23401 taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact
    23461 tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta
    23521 ctcactcctt ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt
    23581 ttataaacag actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc
    23641 agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt
    23701 catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga
    23761 taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga
    23821 tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt
    23881 ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa
    23941 aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg
    24001 gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc
    24061 gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct
    24121 ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat
    24181 ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac
    24241 aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac
    24301 agcctcaagc gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc
    24361 ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg
    24421 ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc
    24481 ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac
    24541 gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa
    24601 gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg
    24661 ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat
    24721 acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg
    24781 tatgttgata tcgccagacg tcatggcctt gatcctgctc agatggcgct cgcgtttgta
    24841 cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa
    24901 actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg
    24961 gtacatcagg tttatactta tcctgcacca taaagattaa cgccggttaa aaccggcgtt
    25021 aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc aaatagcgca
    25081 ccgaagccaa tgccaatggg cacgaccggg atgcctacca ttaccgccag cgagtaaatg
    25141 cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat cgcattcccc
    25201 gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac gacaaaaaag
    25261 ccccccagca cgccaatcag catcaacaag gcataggctg gcagcagctc gtgttgcagg
    25321 gagaaaatcg gtaccaccac gccaatcaaa atcccggctg gcatacagcg tgacacggtt
    25381 tccagcgtaa ccagcttcgc tgccgcacct gcgccaacca cgataccaat cgctaccatc
    25441 gcgttgagat aggtcggcgt agcgttatcg gtaatgccca gcgccaccgg tacccacagc
    25501 accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc caccagcgaa
    25561 aaacgcgttt caccattgca ccatagcgag gtgcaggcat tcaggaaact gcgggtcatg
    25621 ttgatgagat tccaggactg ccccggacgc gccgccagtt tgggaatgta gatattggca
    25681 acgaccgcac caccgtaggc cagtgcgcat gcggccagag cgaccaggac atgccagtca
    25741 gccagcacac caccggctac ggaaccgagt aaaatcgccg ctatggcaga agcttccatt
    25801 aaaccgttag cttttactaa cttactaccc gtggttaatt cgccgagaat accgtatttc
    25861 gccggtgaat aggctgcagc accaacaccc accagcgtat agccgagaaa cggattgata
    25921 ccaaagcaga tactggctgc gcccagcagc ttcaggccgt tggcaaacat catcacccgg
    25981 cctttggcga agctatccgc cacctgcccg acaaacggcg ccaaaagaat gtaagcacct
    26041 acaaacacca tttgcaggat gggctggctc cactccggat agaactgcgc tttcagtaac
    26101 gccagagtgg caaacagtag cgcgttatcg ccaaacgcag agagaaactg cgccacgata
    26161 accgctttca tccccttcga ccacaacgaa gtgttagtgt gcactgactc actcatcgtg
    26221 ttgttccgct tcgtctaccc agcttttcaa cgtgacaaag tcaggtttgc cgctgccaag
    26281 taatggcatc tgtttcagat agcgaatatc gcgcggtaca gcaagctccg gcacgccgtg
    26341 ctcgcgggca tactgttgca acttatcgcg cgtcagttcg ttatctgtgg tgaaaagcac
    26401 cagtgcctcg cctttgctgg catcgctctt aatcgcagtg gcatggactt tatctggcga
    26461 aacaccaagt gccagttgtt ccaccatttc cagcgacacc atttcgcctg caattttggc
    26521 aaagcgtttt gcgcggccct gaatctgcac aaagccctgc tcgtcaaaac gcacaatatc
    26581 gccagtgtca taccagccgc gctccatttc gccgcgaaca ttctcggcgg tgggcacttc
    26641 cagtacacct ggcttctcca cccgcagata gccgttcatt atgttcggcc ctttcagttg
    26701 caggcgtccg ccctcttcga taccagggac cgacaacagg cgtgcatcca ttcctggtag
    26761 aatacgccct accgtaccgg gtttcgccgc catcggtacg ttgatagaaa cgacaggcgc
    26821 gcattcggtc acaccgtagc cttcaaggat gcgcaggcca aatttatcct gccaaagctg
    26881 tttggtactt tcttgtagtt tttctgcgcc tgccaccaca tagcgtagac gatagaagtc
    26941 atacgggttg gcgaaacgcg cgtagtgacc gaggaaagtc gaggtaccga acaacacggt
    27001 gcaactgcgg tcatacacca agtccggcac aatgcggtaa tgcagcgggc ttggataaag
    27061 gaacacttct gcacctgtaa gcagtggcgt aaacaggcct accgtcagcc caaaggagtg
    27121 aaacagcggt aatgccgaca taaagcgatc gttggtggtg aagtcggcaa tcgttttaat
    27181 ctgctcgacg ttcgccagaa tgcttttatg gctatggacg acgccttttg gatggccttc
    27241 agaaccggag gtaaagagga tcagcgcctc ttcttccggc tgctgtttta cctgcgccag
    27301 gcgcggcatc agcaaatgag cgaagatcca tactttgtcg gcagtggtga catctgcttt
    27361 taaatcttcc agatagaccc agcgcacctg agtaagttgc tccggcagat gccagagttt
    27421 gcctttatcg agaaactggc gggaagtgaa gatggttttg atttcagccg ccgtaatagc
    27481 actggtcagc ccttttaccc cggcagtgta gttcatcatt gcgggaatgc ggcgacgcgc
    27541 gatggcccca aaaatcactg ccgcactgat gcctgcattg ggcagcatta agccgatgcg
    27601 ttcgccttca acactgtatt tttcaaggat gcgtccaaca aacagcgttt tcgtaagcaa
    27661 tttgcgatag gagtctgggg taaagttgac gtcttcgaca catttcttcc cggctccgaa
    27721 gcggtacatt gcactcagta aagattcgta cagagtttca cgcgggcgca ccgccatacg
    27781 cgcttccatc attatttgat gcagcatttc gccagcgatt ttgcgacggt cacgggcacg
    27841 cggcgcatcc ggcatcgcca cctgcgttgg aggcaaaata tgcagagtaa tttgcgggaa
    27901 caagcgacgt ttaaccagac ctttcaggcg gctgaagtgc gtaagttccg ccccttcaat
    27961 acgcacagga ataaccgttg caccagactt cgccgcgaca aaacccgcgc catcgtagat
    28021 tttcatcagc gagcctgtag tggtgatgcg tccttcaggg aaaatcacca ctggtcggcc
    28081 ctgttccacc agacgtacca gatgtttaat agccataggt tgcgtcgggt cgagaggaac
    28141 aaagtcgata aatgatttca gccaacgcat ataccactgt tggcttattg aggtgtaaac
    28201 ggcaaacact ggacgcacag gtaaaaacag tccaagcaaa atgccatcaa taaaagagac
    28261 gtgattaggc gtaattagta cgcgctcgcc cttcagtgcc tgggtgtcac ccgtaacgcg
    28321 aacgcgatac aaaacacggc acaaatttcg aaaaaagcta aaaagcattc caactccctt
    28381 tgctctgatt cagtaaaagc gaatggaggg agattacacg agataaagaa cgcgagcgac
    28441 agtaaattag gtgcgaaaaa aaacctgcgc atccgcgcag gttggtgcaa gagacagggt
    28501 acgaagagcg taccgaataa tctcaccaat caatacctct gggatcttga ttgtggtctg
    28561 cacgacgact cttcgccagc gagaaaacgc aaaggaatga agggaaatgc aacgaggtgt
    28621 gtaaattgtc ggttactgtt acagattgat gaccggcaaa aaaaaacctg cgcatctgcg
    28681 caggctggtg taattcatgt gctcaacacg aagttgactt cacctatcaa tacctctggg
    28741 atcaccactt tagcaacctg aagccaaacg ccaccagcgg tcaatcgcaa cagcgtttcg
    28801 caaagtgtaa ccaaaggttt gcaatctctt tttttgtctt acttatttgc gtttggctca
    28861 cattcccacg atgaaaacac gccacccctt gaaccaacgg gcgttttccg taacactgaa
    28921 acaatgtaag cgtttaccca ctaaggtatt ttcatggcga ccataaagga tgtagcccga
    28981 ctggcaggcg tttcagtcgc caccgtttcc cgcgtcatta ataattcacc caaagccagc
    29041 gaagcttccc ggctggctgt gcatagtgca atggagtctc ttagctatca cccgaacgcc
    29101 aacgcccgtg cgctggcgca gcagaccact gaaacggtcg gtctgatcgt tggtgatgtt
    29161 tccgatccgt ttttcggtgc aatggtgaaa gcggtcgaac aggtggctta tcacaccggt
    29221 aatttcttat tgattggcaa cggttaccac aacgaacaaa aagagcgtca ggctattgag
    29281 caactgatcc gccatcgctg tgctgcgttg gtcgtccatg ccaaaatgat cccagatgcc
    29341 gatttagcct cattaatgaa acaaatgccc ggtatggtgc tgatcaaccg tatcctgcct
    29401 ggctttgaaa accgttgtat tgctcttgac gatcgttacg gtgcctggct ggcaacgcgt
    29461 catttaattc agcaaggtca tacccgcatt ggttatctgt gctctaacca ctctatttct
    29521 gacgccgaag atcgtctgca agggtattac gatgcccttg ctgaaagtgg tattccggcc
    29581 aatgatcggc tggtgacatt tggcgaacca gacgaaagcg gcggcgaaca ggcaatgacc
    29641 gagcttttgg gacgaggaag aaatttcact gcggtagcct gttataacga ttcaatggcg
    29701 gcgggcgcga tgggcgttct caatgataat ggtattgatg taccgggtga gatttcgtta
    29761 attggctttg atgatgtgct ggtgtcacgc tatgtgcgtc cgcgcctgac caccgtgcgt
    29821 tacccaatcg taacgatggc gacccaggct gccgaactgg ctttggcact ggcggataat
    29881 cgccctctcc cggaaattac taatgtattt agtccgacgc tggtacgtcg tcattcagtg
    29941 tcaactccgt cgctggaggc aagtcatcat gcaaccagcg actaaccgca gttaaagcaa
    30001 ttccagcgcc agtaattctt cgatggtctg gcgacggcga atcaaccgcg cctgaccatt
    30061 atcaaacaga acttctggta acagcggacg gctattgtag ttggatgaca ttgatgcgcc
    30121 atatgcccct gtatcatgca gtaccagata atcacctgcc ttcacttccg gcaaggcgcg
    30181 ggtttcaaca tttccccctt cctgctgggt aaagacatcg cccgattcac ataacggccc
    30241 ggcgacgacg gtttccaccg ttggcgcgtg ttccagagaa cgaccatcag ctgccagggc
    30301 actgatatgg tggtaactac cgtacattgc cgggcgcatc agatcgttga acccggcatc
    30361 aaccagcaca aagtggcggc tacccatttg tttgacgctc cgcacctgag taattaatac
    30421 gccagactgc gctaccagga agcgacccgg ttcaatttcc agtttcacag ggtggcccaa
    30481 atggcgagcg atttgctcac gcgcggcatt ccacagacca taataatgtt cggtatcaac
    30541 cgcctcttca ccctgttgat aaggaataga aagcccaccg cccgcagaaa tagcctgtaa
    30601 atcctgaccg aattcgataa cctgacgcac catagcacca cacacctgct ccagatgggc
    30661 ataatcaacg ccagaaccaa tgtgcatgtg aatgccgact agctgcagat gatgacgttg
    30721 tatcacgtcc agtgcggcgg gcagatcggt gtaccagata ccgtgcttgc tgttttcgcc
    30781 accggtattg gttttttggc tatgtccgtg accaaacccc ggattaacgc gcagccatac
    30841 ccgatgccct ggcgaaacct ggcccagttg gtcgagcgta tcaacagaac ccgcattcac
    30901 cggaatttgc aattcactga cgcgttcaag cgtcgcctga tcgataacat ctgccgtaaa
    30961 aacaatatca tcggggtgcg tttgcggatt gtaacccgcc gccaacgcac gctctatttc
    31021 gcctaacgag acggaatcca ctttcacgcc ctgctcacgc attaagcgca aaatatgaat
    31081 attggaacag gctttctgtg caaagcgcac cacttcaaac tgtttcagcg ctgcaatctg
    31141 ccgacgaata atttgcgcat cgtagaccca caccgggcag ccaaattcag cgggcaaacg
    31201 cagcagattt tcggcggtga gatcggtatc ggtgctgaac agtgaatgtg gcataacaaa
    31261 ctccagatga gtgctttttt atgattacgc cacatcataa aaagaataaa aaatatcgat
    31321 ttatgtcgag tctatgcaaa aatgatatga attaccggat tgcgagagag cgctaatggc
    31381 cgccgttaac ttacgtcata ttgaaatttt tcatgcggta atgaccgccg gaagcctgac
    31441 tgaggcagca cacctgctac acacctcaca gccaaccgtc agccgcgaac tggcgcgctt
    31501 tgagaaggtg atcgggctga aattgtttga gcgcgtacgt ggacgattac atcctaccgt
    31561 gcaaggactg cgtctgtttg aagaagtgca acgatcctgg tacggactgg atcgcattgt
    31621 cagcgccgca gaaagtctgc gcgagtttcg ccagggagaa ctgtctattg cctgcctgcc
    31681 ggtcttttcg caatcttttt taccgcagct cctgcaaccc tttctggcac gttatcccga
    31741 tgtcagctta aatatcgtgc cccaggaatc accgctactt gaagagtggc tctcggccca
    31801 gcgtcatgat ttaggactca ctgagacact ccatacgcct acgggaacag aacgtaccga
    31861 attactctct ttagatgaag tgtgtgtgtt acctccgggt catccgctgg cggtaaaaaa
    31921 ggtattaacg ccggatgatt ttcagggtga gaattacatc agcctttccc gtactgacag
    31981 ctatcgccag ttgctggatc agctatttac tgaacatcag gttaaacgac gcatgatcgt
    32041 agaaacccac agcgccgcgt cagtctgcgc aatggtacgg gcgggggtag gtatttcggt
    32101 ggttaacccg ctcaccgcac tagattatgc ggcaagcggt ttagtggtgc ggcggttcag
    32161 tattgcggtt ccgttcaccg tcagcctgat ccgccccctg caccgcccgt catcagcgct
    32221 ggttcaggcg tttagtgggc atttacaagc ggggttaccg aaactggtca cttctcttga
    32281 cgctattttg tcgtcagcta cgacagcata aaagcgacag catcctcggc atggatcgcc
    32341 gcggtatcaa acacaggcaa aacactgcgc tcttctggca ccagtaaacc aatttctgtg
    32401 cagccaaaaa tgacgccctg tgcgccctgt tctgcaaggc gagcaatcac ttgcgcataa
    32461 taagcgcgtg acgcttcggt aaattgcccc agacacagtt cttcaaaaat aatctgatta
    32521 attttcgccc gttcatccgc ttcaggaata agacaattga tggaaaattg ttccgtcagc
    32581 cgcccgcgat aaaaatcctg ttccatggtg taacgcgtac ccagcagcgc cacacgagtc
    32641 attccggccc cggtaattgc acgtccggtg gcatccgcaa tgtgtaagaa aggcagagtg
    32701 caacgtgact caatggcatc cgccacttta tgcatcgtat tggtacataa cacaatacct
    32761 tctgcgcccg cccgctgtaa gccaagcgcc gcctcagcca gaatgtcccc ggttttatcc
    32821 cattccccgc gacgctggca ctcttctatt tcatgaaaat cgacgctatg tagcagcatt
    32881 tgcgcagagt gaagcccacc aagccgctgt ttaatgcctt catttatcag acgatagtaa
    32941 ggaatggtgg attcccagct cattcctccc agcaaaccaa ttgttttcat tcctctctcc
    33001 tgatgtgtgt taccgcaaca aggctgaata aggaacgaga caaacgcctc aacggccagg
    33061 tgcccaatct ctattaacga aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc
    33121 agacgccgat atttctcaac ttctcgcctg ccatcagttt gcgttcgata tgttccagcg
    33181 tgacattttt ggtttccgga atgagccaga aagtaatacc cacaaacgca atgttcagcg
    33241 cagtgtagag ccagaacgta ccggcagcgc caatgctatc aagcagtgtc aggaaggccg
    33301 cgccgataat catattcgac acccagtttg tcgtcgtcga acaggtaata ccgaaatcgc
    33361 ggcatttcag cggctgaatt tcagagcaca ggatccacac cactggcgcg gcgctcatcg
    33421 cataaccggc aatacacatc atcgtcatgc caacagagag ccaggacaag ccactggaag
    33481 ccgtaccgtt atcaaactgc atcaggcaat agcccagcac cagagtgcct aacgccatca
    33541 cgctgaaacc aattttcaga gccggtttac gacctgcttt atctaccgta aagaccgcaa
    33601 tgaaggtcgc gaacataaag gtcagtccca cgaccagagt cgcaatcatc tgttgttctg
    33661 tggtcgtaaa gcccgccatt ttgaagatac gcggcgcgta gtacatgatg atgttcatac
    33721 cggtaaactg ctgcatcgcc tgcaacaaca taccgagaaa cacagcacga cggacgttac
    33781 ggttgatctt aaacagtgcc caaccgccct gttttaactt caggctttca cgaatttcgt
    33841 tgagttcttc tcgcgctttt tccgacgtat cgcgcagcat acgcaacact tcttccgcct
    33901 caatatgacg ccccttttcc gccagccagc gcgggctatt tggcaggaag accaccagaa
    33961 taatcagcag aactgctggt aaagcaagaa cccccaacat tgcgcgccag ttaccgctat
    34021 aactgaacgc tgtatcggat aaaaacgcca gcacgatgcc gagtgtgacc atcaactggt
    34081 acatactgat catcttaccg cgaacgtttt cacttgccat ttcagaaaga tacagaggag
    34141 cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac acgagcggcg attaacatct
    34201 ctacgctggt cgcaaaagcg gaccctatag aaccgagtac aaacaggatg gcccccgcca
    34261 tcaggctgta tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg
    34321 ctgcgccgag catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt
    34381 gatcggtaat gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc
    34441 ctgcgaccgc agcagctacc gaaacaaaca tattcatacg ccgcgtatca cgcaaagaac
    34501 gtggcgttaa agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa
    34561 gacattgtga aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa cagcataaat
    34621 atggattaaa ttgctgcgac atgccgttat gtgatggata ttccaatttt aaaattaagt
    34681 tgaattattg agattattat taacctccta attttaaagc agataaaatt cataaagttc
    34741 attaattgat aattgatatg gattatttca taaccatgat atggattatg atgatctaga
    34801 gatataaaaa accctgccat gcggcagggt cataaaagta agaggaatga attaacgcgc
    34861 cagccaaccg ccatccacag caatggtata accatttaca taatctgaag cgctggaggc
    34921 aagaaacact accggcccca tcaggtcact cggcagtccc caacggccag ctggaatgcg
    34981 atcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgggtat tgttggtcgc
    35041 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgtttgccat
    35101 caatcgcgtc acacccatca cgccgctttt tgatgcggta taagaaggca cacggatccc
    35161 gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat
    35221 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
    35281 atcgtcccag tctttttcgc tgaactcgag agcgtcttcg cggcgaatca atccggcgtt
    35341 attcaccagg atatcaatat gaccaaattc agctaccgcg cgatccagca gtccaggaat
    35401 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
    35461 ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
    35521 cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac
    35581 tttaccttgg agagaaaatg cacttaaaat cataacaaca ccttattcgt ttatgcccac
    35641 aactagcgca aatctttaac gcccacatgg tccatatcat taaagacctg gttttcaccg
    35701 accatgcccc agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctc
    35761 ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc
    35821 atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg
    35881 cgctcgtggg tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
    35941 cccatactca attgacaggt ttccagtaca tccgggacaa aatatttgtt aatcgtgcga
    36001 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
    36061 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg
    36121 gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg
    36181 tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata
    36241 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
    36301 ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg
    36361 ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattgcgc
    36421 agcccttggg tatccagcgt ttttgcgtgc gcaccgtgga tgctctgtct tacgtccaca
    36481 tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt
    36541 tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat
    36601 gttttatttt taatttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
    36661 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
    36721 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
    36781 aatggtcaat gccacgccct ggcccccgcc gatacaaagc gttgccagcc ctttgcgggc
    36841 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
    36901 cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag
    36961 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
    37021 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
    37081 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
    37141 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
    37201 tatggatgat gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg
    37261 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
    37321 ctgaccgtta ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
    37381 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
    37441 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
    37501 ccacaaccca tcatgcacca gactgtcaac caactggcta ttgccaagct gttcaccggt
    37561 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac ctgcgatgac
    37621 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagaccgga
    37681 accgcaaacg tcattaatcg tgattgcaga aacgctgtta ggaagaccac ctttaatagc
    37741 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
    37801 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
    37861 aagttccacg gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa
    37921 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
    37981 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
    38041 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
    38101 accaatagat gggtagatag ggcgttaatc tcccatactt aacttggttt atggtaaatt
    38161 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
    38221 aagaagaaac tctgtgaagt ttcgcgctct atggcacagc cgttgatgca ggcacgctat
    38281 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
    38341 cctggccttt aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcaaaaa
    38401 catcagcagg tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta
    38461 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
    38521 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
    38581 gtatgttggt gagtctgggt gttgtattaa ttctgaatct catttttctg atgggacgtc
    38641 atgccactat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
    38701 tttccattta ccttgtcggt agttggcaac ctgatctatt aacaacccag gtagagttca
    38761 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
    38821 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
    38881 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
    38941 tctttgtctt tagctgcctg ctttctattc catcttcgta tattgaagcg gctaaagaag
    39001 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa
    39061 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
    39121 gcgttattga aggtgccaca gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
    39181 aaagtcgtgc atttaaccac gcactatcaa ttatgttggt atcgctgatt accttcattg
    39241 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
    39301 ccatgatact tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct
    39361 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
    39421 tctttagcta atatccgttg tggatggttc aaaattgcta caattttgaa ccattccatt
    39481 tttcataata ttcatttgtt attattccgc cctatcactt ctctcgtcat atcctagaat
    39541 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
    39601 ataacttaga ttcgcaagat atcttctggt cattcaaaaa caatttgcga tatagatatt
    39661 ctcataccat gagatagtta tctggaccac gttcgaccat gcaagattaa atcagtgaaa
    39721 cataatcata tttaataacg agaaatgcat ttttaaatat cttaatcaga attatagcaa
    39781 atacagatta atcgcaattt ttcccagcaa aattacaaaa tcaaggtgta ttgacatcat
    39841 caatcaccaa tgaaattatg caattatata cggataggga ggttcttaac atggggcttt
    39901 gtagtcgtta taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag
    39961 aagagtcacc gcgtttgcag tactgcgcaa actcgtgtt
//
LOCUS       DAJVPT010000026        39154 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVPT010000026 DAJVPT010000000
VERSION     DAJVPT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..858)
                     /gene="rpnA"
                     /locus_tag="OQM47_001320"
     CDS             complement(<1..858)
                     /gene="rpnA"
                     /locus_tag="OQM47_001320"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1479539.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEE"
     gene            complement(1061..1297)
                     /gene="feoC"
                     /locus_tag="OQM47_001321"
     CDS             complement(1061..1297)
                     /gene="feoC"
                     /locus_tag="OQM47_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1479540.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1297..3618)
                     /gene="feoB"
                     /locus_tag="OQM47_001322"
     CDS             complement(1297..3618)
                     /gene="feoB"
                     /locus_tag="OQM47_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1479541.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3635..3862)
                     /gene="feoA"
                     /locus_tag="OQM47_001323"
     CDS             complement(3635..3862)
                     /gene="feoA"
                     /locus_tag="OQM47_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1479542.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4300..6621)
                     /locus_tag="OQM47_001324"
     CDS             complement(4300..6621)
                     /locus_tag="OQM47_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1479543.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6718..7194)
                     /gene="greB"
                     /locus_tag="OQM47_001325"
     CDS             complement(6718..7194)
                     /gene="greB"
                     /locus_tag="OQM47_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1479544.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7422..8141
                     /gene="ompR"
                     /locus_tag="OQM47_001326"
     CDS             7422..8141
                     /gene="ompR"
                     /locus_tag="OQM47_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1479545.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8138..9490
                     /gene="envZ"
                     /locus_tag="OQM47_001327"
     CDS             8138..9490
                     /gene="envZ"
                     /locus_tag="OQM47_001327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1479546.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9566..11188)
                     /gene="pckA"
                     /locus_tag="OQM47_001328"
     CDS             complement(9566..11188)
                     /gene="pckA"
                     /locus_tag="OQM47_001328"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1479547.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11567..13318
                     /locus_tag="OQM47_001329"
     CDS             11567..13318
                     /locus_tag="OQM47_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1479548.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13462..14331)
                     /gene="hslO"
                     /locus_tag="OQM47_001330"
     CDS             complement(13462..14331)
                     /gene="hslO"
                     /locus_tag="OQM47_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1479549.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14356..14757)
                     /gene="hslR"
                     /locus_tag="OQM47_001331"
     CDS             complement(14356..14757)
                     /gene="hslR"
                     /locus_tag="OQM47_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1479550.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14768..15436)
                     /gene="yrfG"
                     /locus_tag="OQM47_001332"
     CDS             complement(14768..15436)
                     /gene="yrfG"
                     /locus_tag="OQM47_001332"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1479551.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15501..17636)
                     /gene="igaA"
                     /locus_tag="OQM47_001333"
     CDS             complement(15501..17636)
                     /gene="igaA"
                     /locus_tag="OQM47_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1479552.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17956..18516
                     /gene="nudE"
                     /locus_tag="OQM47_001334"
     CDS             17956..18516
                     /gene="nudE"
                     /locus_tag="OQM47_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1479553.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18681..21233)
                     /gene="mrcA"
                     /locus_tag="OQM47_001335"
     CDS             complement(18681..21233)
                     /gene="mrcA"
                     /locus_tag="OQM47_001335"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1479554.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21353..22132
                     /gene="hofM"
                     /locus_tag="OQM47_001336"
     CDS             21353..22132
                     /gene="hofM"
                     /locus_tag="OQM47_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1479555.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22132..22671
                     /gene="hofN"
                     /locus_tag="OQM47_001337"
     CDS             22132..22671
                     /gene="hofN"
                     /locus_tag="OQM47_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1479556.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22655..23095
                     /gene="hofO"
                     /locus_tag="OQM47_001338"
     CDS             22655..23095
                     /gene="hofO"
                     /locus_tag="OQM47_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1479557.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23085..23491
                     /gene="hofP"
                     /locus_tag="OQM47_001339"
                     /pseudo
     CDS             23085..23491
                     /gene="hofP"
                     /locus_tag="OQM47_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23403..24641
                     /gene="hofQ"
                     /locus_tag="OQM47_001340"
     CDS             23403..24641
                     /gene="hofQ"
                     /locus_tag="OQM47_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1479558.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25042..25563
                     /gene="aroK"
                     /locus_tag="OQM47_001341"
     CDS             25042..25563
                     /gene="aroK"
                     /locus_tag="OQM47_001341"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1479559.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25620..26708
                     /gene="aroB"
                     /locus_tag="OQM47_001342"
     CDS             25620..26708
                     /gene="aroB"
                     /locus_tag="OQM47_001342"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1479560.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26800..28092
                     /gene="damX"
                     /locus_tag="OQM47_001343"
     CDS             26800..28092
                     /gene="damX"
                     /locus_tag="OQM47_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1479561.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28199..29035
                     /gene="dam"
                     /locus_tag="OQM47_001344"
     CDS             28199..29035
                     /gene="dam"
                     /locus_tag="OQM47_001344"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1479562.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29053..29730
                     /gene="rpe"
                     /locus_tag="OQM47_001345"
     CDS             29053..29730
                     /gene="rpe"
                     /locus_tag="OQM47_001345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1479563.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29723..30481
                     /gene="gph"
                     /locus_tag="OQM47_001346"
     CDS             29723..30481
                     /gene="gph"
                     /locus_tag="OQM47_001346"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1479564.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30474..31478
                     /gene="trpS"
                     /locus_tag="OQM47_001347"
     CDS             30474..31478
                     /gene="trpS"
                     /locus_tag="OQM47_001347"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1479565.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31748..32653
                     /locus_tag="OQM47_001348"
     CDS             31748..32653
                     /locus_tag="OQM47_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479566.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32670..33032
                     /locus_tag="OQM47_001349"
     CDS             32670..33032
                     /locus_tag="OQM47_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1479567.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33116..34279
                     /locus_tag="OQM47_001350"
     CDS             33116..34279
                     /locus_tag="OQM47_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1479568.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34280..35506
                     /locus_tag="OQM47_001351"
     CDS             34280..35506
                     /locus_tag="OQM47_001351"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1479569.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35503..36381
                     /locus_tag="OQM47_001352"
     CDS             35503..36381
                     /locus_tag="OQM47_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1479570.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36392..36745
                     /locus_tag="OQM47_001353"
     CDS             36392..36745
                     /locus_tag="OQM47_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1479571.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36757..38061
                     /locus_tag="OQM47_001354"
     CDS             36757..38061
                     /locus_tag="OQM47_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1479572.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38073..>39154
                     /locus_tag="OQM47_001355"
     CDS             38073..>39154
                     /locus_tag="OQM47_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1479573.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIAS"
BASE COUNT         9089 a         9870 c        10739 g         9456 t
ORIGIN      
        1 ctcttcttct gacagcccgg taaatcgcat gatgtctgca agagggactc cggattcaag
       61 cattattttg gctatatgca gggctttgga ttgttccccc tcctgccgca atctttccgc
      121 aatagtcatt aaactctcct tgtgtttcgg tgaacgttcg gcaacgccgt cgataaaatc
      181 gttaaaacgt acagcgtcgc cagtttgcag tatgtaatta aacagccctt tgatttgtct
      241 gtcattagcg tatccactac ttaataagca ggccatttgc tctaccagcc ccatcagatc
      301 gcgttgacga atatgttttt gaattaactc cagcagcgcc atacgtcggt gctgcatgat
      361 ttcatcatca ggcatgacgg tgacatcaat cagcggaaat gcggaggcat aaagctgcct
      421 tgccagtttg ggatcggcga aacaatccag ccaacacagc gaatagggat aggggctttc
      481 aataccgtgg taaaacaaca atggcaccac catcggcaac gttttgtatc cagcatcaag
      541 gtgattttgc attgcggcaa tagcgtaacg catcatgcga aatgcgatca gtttgtttga
      601 ggtgctttga tgttcaatca gacaatagat gtatcctggt ccttgttccg ttttcaccga
      661 ccacagcaca tcggaatagc tttcacgcag atcgtcatca ataaagctcc tcgactccag
      721 cttgagggtt ttcatatcac agagcgcgtg aatgggtgcc ggtaaatgaa acgcaagaaa
      781 atcgcgtgcc gtctccggtt ggcgtaaaaa gagtttgaac agcgcatcat gtggggtgga
      841 actctgcttt ttactcatgg cgttccttca cctgttgttt gaatgccgcg actctacgcg
      901 ccactccaca tctgtgcatt agcgagtttt catccctgcg agacgctttc agaggaactt
      961 acgttgcgct gcaagaattg catttttatg gaaagccgca cgcaaaaaag cccggatgca
     1021 aacgcatacc gggcttttca aaacaggcga tggagtatgg ttaacgcagc gcccaccact
     1081 cgcgcagaca ggctttgcct tccgggcagc ttttacaact gccagagagg cagccgtcag
     1141 gttcttcctg aatccgcacg gctttgccca tgctttccag ctgttgcagc atggcgttaa
     1201 tcattggctg cggagtgttc aatgtctggc ttatctgggt cgcttccata cggccccgta
     1261 acgccagcaa atcacgtacc tgaataagtg aagccattaa tggcaatcac cggtggtgct
     1321 ggctgcgcag caactgctta ccgacttgcg ggttgccagc agttcgatat ccacccggct
     1381 acgcgcgcgg cgcagcagac cgatagctac gatgttaaac aggattaccg ccagaatgca
     1441 caccaggctg taagttgggt gctgactgta gctggcgacc tgatagaaca atgttgccag
     1501 tgagtaagcg atattcagcc cccacaggat ggagaagccc atccagccac ggcttgattc
     1561 acgggcgatg gctcccatca ccgagataca tggtacatac agcaggacga aaatcaggta
     1621 gctgtatgcg gcagctgcgc taccgaattt ctgatccatc acgcccatcg ccccggtacc
     1681 catttcaccg ttgcctttgc tagcttcaat ggggttcatc agtacgctaa ggctgaaggt
     1741 gtctttcagg ctctgccagg tttcatctac cgcactgaac agctcttcac cgaggttgaa
     1801 ttccgccgga ttgaactctt cgtcctgaat attttctgcg gtgtagaggg tgttgagcgt
     1861 acccaccacc acttcttttg ccattgcgcc cgtaaacagg ccaaccgttg cctgccagtt
     1921 atcttcatgt acgccaatcg gcttgaagac aggggttatc acccggctga cggaagccag
     1981 cgccgagtcg ttgatgttat cgacgatttt cccgctcaac gagaagctgt tgaaagcgct
     2041 caggaaaatg ctgacgatga taatcacttt accagcacgc agaacgaagc ccttcagacg
     2101 ctgccaggtc tggataatca ggcttttaac gtgtggtaca tggtagaccg gcagctccat
     2161 gacaaacggc gtcgcttcac cgcgcatgat ggtgtacttg agcatcaggc cagtcagcac
     2221 cgccatcacg ataccgagca tatacagcga gaaaaccgcc agggcaccgt tctgcccgaa
     2281 gaaggcagcc gcgaatactg cgaagatagc cagacgcgcg ccgcaggaca taaacggtgc
     2341 catcatgatg gtcatcagac gttcacgcgg tgcatcaagc gtacgtgcgc ccattaccga
     2401 cggtacgtta caaccgaaac cgacgatcag cggcacaaag gatttccccg gcaagcccag
     2461 cgcctgcatc aggcggtcca tcacaaacgc tgcacgcgcc atatagccgg agtcctcaag
     2521 gaaggagagg aacaggtaca tcatgccgat ctgcggcacc agtggcagca cggtgttaat
     2581 accgccaccc aggccctggg cgaggaagat agtcagccag tccgggaagt ggagcgtgta
     2641 gccaatccat tgaataccat gcacaaatag cgccacggag ccgacgttaa acagcggctg
     2701 taacgccccg ccgatgttga tagccagcag gaacatcagg tacatcacaa agaggaaaat
     2761 cggcagaccg aggaaacggt tgagcacgat tttatctacc gcagtggtga aacggctggg
     2821 ttctgccgtc agggtgttgc ttaccacatc acagatggca gcaatgcact ggtaacgcgc
     2881 atcggcaatg tgcagcgccg gatcatccat ttcattacgc agacgggcga gggtggcatc
     2941 cagatgctgc gacgcttcac cggcgtaggc gcggctgtag atatcgcctt ccagcatttg
     3001 caggcccagc cagcgacgtt gtttcagcgg gatgtcggaa ggcatcactt ttgccagtga
     3061 atctgcttcg ttgagtagcg gctgtgcgta atgcaccagt tccacattct cgttagcttt
     3121 atagcgatca atcgccagct tgagcgcttc aataccgcga ccacgggttg aaaccagcgg
     3181 gatcaccggg cagccaagac gtgccgatag agcatcaatt tcaatacgaa tattttgctt
     3241 ctcggcaatg tcgagcatgt tcagcgccac gatgcaggga atgccgagtt ccagcagttg
     3301 cagcgtcagg tacaggttac gctcaaggtt agacgcatcc accacgttaa tcagcaggtc
     3361 ggcgtcgcca ctcaaaatgt agtgacaggc gatttgctca tcgagcgagg tctgcgatga
     3421 gatggtggtc agagaatagg tgccgggcag gtccaccagc gtgacctgat gatcggtggt
     3481 ggagaattgc ccttctttac gttcgacggt aacgccagcc cagttaccta cacgctgacg
     3541 tgcgccagtg agctggttaa ataaggttgt cttgccagaa tttggattac caattaagcc
     3601 aatggttaat tttttcattg ttgttatcac cgtattaaca ggaaaccgct tccacttcta
     3661 ataaggccag atcttttttg cgtaatacca ggctcacacg acgggtttcg atatgaatgg
     3721 ggtcgccgag tggagcgacg cgcaccacat caaaagagga gccaggtaac atgcctagag
     3781 aaagcagttt ttggcgatat gccgggctga tttcacggga aaagccagtg attttccacg
     3841 cagtatctgg agtgtattgc ataggtgcct acttgtttct cattaactgg ataactacct
     3901 catggggcgt ttgaccgcga catgagacca gatgatttgc caacaaagga gagattaaat
     3961 aatcaacaac acaatgataa tgagaatggt ttctatcagc aatacttaaa atgtgtgcga
     4021 atgttggttt gaataataat ctgactcaat gttgatgtgg ctcaaggtta tatcgcagcg
     4081 aaaatataat tcgaatagtg agttacccgg actggctaat gtttaattaa ggtttcagtt
     4141 gttaatgaag tgataaatat taacggagta tattgtgagt ttggctgtgg aataaaagtg
     4201 ccggagatat tctccggcac ttttatgtca ggcaaagcgt tcgttgccag atgcggcgtg
     4261 aacgccttat cgcgcaggtt taggggccag tccgggcgtt taacgttttt tacccatcgc
     4321 tgccgccagc gcatccatca tcgcgctatt accggcaggc tgcgcttcac gaccgcgtgg
     4381 tttggctgcc gggcggttgt tttgcgggcg ttcattaccg ccgccgcgac gagagttggt
     4441 ttcgccaggc tgctcgtcca gacgcatagt cagggcgata cgtttacgct gaagatccac
     4501 ttccagcact ttcaccttca caatgtcgcc cgctttcacc acggtatgcg gatcttccac
     4561 aaacttgttc gacaatgaag agatgtgaac caggccgtcc tgatgcacgc caatatcgac
     4621 aaacgcgcca aagttggtga cgttggtcac tgcgccttcg aggatcatac ccggttgcag
     4681 gtcgttcatt gtctcgacgc catcggcaaa ctgagcggtt ttaaattccg gacgcggatc
     4741 gcgacccggt ttttccagct ctttgatgat gtcagttact gtcggcacac cgaatttttc
     4801 atcagtaaag tcagacgctt tcaggttacg cagttcgctg ctgttaccca tcagatcttt
     4861 cagtgcctgc tgtgttgctg ccagaatgcg ttccaccacc ggataggctt ccggatgaac
     4921 ggtagaggcg tccagcgggt tatcaccgtg gttaatgcgc aggaagcccg cgcactgctc
     4981 gaaggctttc ggccccagac ggctgacttt cagcagttgc tgacggttct ggaactggcc
     5041 gttctcatcg cgccaggcca cgatgttttg cgccatcatg cgcgtcaggc ccgccacgcg
     5101 ggttaatagc ggaacagaag cggtgttgag atcgacgcca acggcgttta cgcagtcttc
     5161 tactactgca tccagtttgc gggccagttg cgtctggctg acgtcatgct gatactgacc
     5221 tacgccgata gatttcggat cgattttcac cagctccgcc agcggatcct gcaaacggcg
     5281 ggcgatagat accgcaccac gcagcgaaac gtcgagatcc gggaactcct gcgctgccag
     5341 ctcggaagct gagtaaaccg acgcgccagc ttcgctgacg atcactttct gtgcggtcac
     5401 tttcgggaac tgcttctgca cgtcgagata gaaacgttca gtttcgcggg aagctgtacc
     5461 gttgccgatc gccaccagtt caacgttatg tttttcacac aaggcagcaa cggtcatcgc
     5521 tgcttttgcg gcttgtccgg tgtgcgggta aatggtatcg gtcgccacca gtttgccagt
     5581 ggcatcgacc accgccactt ttaccccggt acgcagaccc ggatcgaggc ccatcgttgc
     5641 acgcagtccg gcaggggccg ccatcagcag atcgtgcagg ttacgggcaa agacgttgat
     5701 tgcttcatct tccgcgcgtt cgcgcacggt acccatcagt tcggtttcca gatgcatcag
     5761 caccttgatg cgccaggtcc agctcactac gcttttgcgc cagctatccg ccggggcatt
     5821 gttcaggcgc aggccaaggt gatccatgat gatttgctcg caatagctct ctttgggcgg
     5881 ctcatcgaac tgcggatcgg cattcagcga aagctggagt acgccttcgt tacgcccacg
     5941 gaacatcgcc agcgcgcggt gagaaggcac cgtggacaac ggttcgtgat gatcgaaata
     6001 gtcgcggaat ttcgcccctt cctcttcttt accgctcacc accgtagaaa ccaaatgcgc
     6061 gttcttccac agataatcac gcactttcgc cagcagcgcg gcatcttcgg caaaccgttc
     6121 catcaggata tagcgcgcgc catccagcgc ggctttggta tctgccacgc ctttatcggc
     6181 atcaacatat tgtgcagcgg cgacttctgg cgtgtgtgac ggatcgctcc acagcaggtc
     6241 agccaacggc tcaagccctg cttcaatggc gatttgcccg cgggtgcggc gtttaggttt
     6301 gtagggcagg tagaggtctt cgagttcggt tttgcttagg gtggcgttga tggccttcgc
     6361 cagatcatcg gtgagtttgc cttgctcgga aatggatttg aggatcgcct gacgtctctc
     6421 ttccagctcg cgcagatagc tcagacgcgt ttccagatta cgcagctgcg tgtcatccag
     6481 accgccggtg atttccttac gataacgtgc gataaacggc acggtattcc cttcgtcaag
     6541 caggcgaacg gcagcgtcaa cctgttccgg gcgcgcctga atttcacccg caataatgcg
     6601 gcagaacgaa tcattcatca tggtttgagt tcatcttttc ggatcaaaaa tcaggggata
     6661 gttatacgga ctggctggca aaaatgccag ccatcggcag gaggttaaga ctcttcctta
     6721 cggtttcacg tactcgatag cattaacata ccagttcgct tccccggcag gggtattcac
     6781 caccgccaga tcgccgactt cttttttcag caatgcgcgg gccatcgggg aatcgataga
     6841 gatgtaatct ttacggccaa aaatttcatc gtagccgaca atacggaaac ggtgagtcac
     6901 gccatcgtcg ttttcaatct ccacccacgc gccaaaaaag actttgcctt cctgctgagg
     6961 ggagtaatcg acgattttga gattttccag gcatttagtg agatagcgca cgcgacggtc
     7021 gatttcacgc agacgctttt tattatactg atagtcagca ttttcgctgc ggtcgcccag
     7081 acttgcggcc caggtcacct tttttgtgac ctccgggcgt tcttcacgcc agagataatt
     7141 aagctcttgt ttgagttttt catacccttc ccgggtaacc aggggcgttt tcatctcgtt
     7201 gattcccttt gtctgtttga taatgcgcac attgggtata acgtgatcat atcaacagaa
     7261 tcaataatgt ttcgccgaat aaattgtata cttaagctgc tgtttaatat gctttgtaac
     7321 aatttaggct gaaattcata ccagatttag ctggtgacga acgtgagctt ttttaagaat
     7381 acacgcttac aaattgttgc gaacctttgg gagtacaaac aatgcaagag aactacaaga
     7441 ttctggtggt cgatgacgac atgcgcctgc gtgcgctgct ggaacgttat ctcaccgaac
     7501 aaggcttcca ggttcgaagc gtcgctaatg cagaacagat ggatcgcctg ctgactcgtg
     7561 aatctttcca tcttatggta ctggatttaa tgttacctgg tgaagatggc ttgtcgattt
     7621 gccgacgtct tcgtagtcag agcaacccga tgccgatcat tatggtgacg gcgaaagggg
     7681 aagaagtgga ccgtatcgta ggcctggaga ttggcgctga cgactacatt ccaaaaccgt
     7741 ttaacccgcg tgaactgctg gcccgtattc gtgcggtgct gcgtcgtcag gcgaacgaac
     7801 tgccaggcgc accgtcacag gaagaggcgg taattgcttt cggtaagttc aaacttaacc
     7861 tcggtacgcg cgaaatgttc cgcgaagatg agccgatgcc gctcaccagc ggtgagtttg
     7921 cggtactgaa ggcactggtc agccatccgc gtgagccgct ctcccgcgat aagctgatga
     7981 accttgcccg tggtcgtgaa tattccgcaa tggaacgctc catcgacgtg cagatttcgc
     8041 gtctgcgccg catggtagaa gaagatccag cgcatccgcg ttacattcag accgtttggg
     8101 gcctgggcta cgtctttgta ccggacggct ctaaagcatg aggcgattgc gcttctcgcc
     8161 acgaagttca tttgcccgta cgttattgct catcgtcacc ttgctgttcg ccagcctggt
     8221 gacgacttat ctggtggtgc tgaacttcgc gattttgccg agcctccagc agtttaataa
     8281 agtcctcgcg tacgaagtgc gtatgttgat gaccgacaaa ctgcaactgg aggacggcac
     8341 gcagttggtt gtgcctcccg ctttccgtcg ggagatctac cgtgagctgg ggatctctct
     8401 ctactccaac gaggctgccg aagaggcagg tctgcgttgg gcgcaacact atgaattctt
     8461 aagccatcag atggcacagc aactgggcgg cccgacggaa gtgcgcgttg aggtcaacaa
     8521 aagttcgcct gtcgtctggc tgaaaacctg gctgtcgccc aatatctggg tacgcgtgcc
     8581 gctgaccgaa attcatcagg gcgatttctc tccgctgttc cgctatacgc tggcgattat
     8641 gctattggcg ataggcgggg cgtggctgtt tattcgtatc cagaaccgac cgttggtcga
     8701 tctcgaacac gcagccttgc aggttggtaa agggattatt ccgccgccgc tgcgtgaata
     8761 tggcgcttcg gaggtgcgtt ccgttacccg tgcctttaac catatggcgg ctggtgttaa
     8821 gcaactggcg gatgaccgca cgctgctgat ggcgggggta agtcacgact tgcgcacgcc
     8881 gctgacgcgt attcgcctgg cgactgagat gatgagcgag caggatggct atctggcaga
     8941 atcgatcaat aaagatatcg aagagtgcaa cgccatcatt gagcagttta tcgactacct
     9001 gcgcaccggg caggagatgc cgatggaaat ggcggatctt aatgcagtgc tcggtgaggt
     9061 gattgctgcc gaaagtggct atgagcggga aattgaaacc gcgctttacc ccggcagcat
     9121 tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg gcgaatatgg tggtcaacgc
     9181 cgcccgttat ggcaatggct ggatcaaagt cagcagcgga acggagccga atcgcgcctg
     9241 gttccaggtg gaagatgacg gtccgggaat tgcgccggaa caacgtaagc acctgttcca
     9301 gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc acgggattag ggctggcaat
     9361 tgtgcagcgt atcgtggata accataacgg gatgctggag cttggcacca gcgagcgggg
     9421 cgggctttcc attcgcgcct ggctgccagt gccggtaacg cgggcgcagg gcacgacaaa
     9481 agaagggtaa ataaacggga ggcgaaggtg cctcccgttt tgctttctat aagatactgg
     9541 atagatattc tccagcttca aatcattaca gtttcggacc agccgctacc agcgcggcac
     9601 ccgcaggggt gtcggtgtat ttatcgaagt tgtcgataaa cagtttcgcc agggtttcgg
     9661 ctttttcctg ccactgttcc ggagaagcgt aggtgttacg cggatcgaga atcttcgtgt
     9721 ctacgcccgg cagttcggtt gggatcgcca ggttaaacat cggcagagtg aaggtttctg
     9781 cattatccag cgaaccgttg aggatggcgt cgataatggc gcgggtatct ttaatcgaga
     9841 tacgtttgcc agtgccgttc cagccagtgt taaccagata agcctgcgcg cccgccgcct
     9901 gcatacgttt caccagcact tctgcgtact gagtcgggtg cagcgacagg aacgccgcgc
     9961 cgaagcaagc ggagaaggtt ggcgtcggtt cggtgatgcc acgctcagta ccggccagtt
    10021 tggcggtgaa gccagagagg aagtgatact gggtttgatc ggcagtcagg cgagaaaccg
    10081 gcggcaacac gccgaaagca tcagcagtca ggaagataac cttagtcgcg tggcccgctt
    10141 tggaaaccgg cttaacgatg ttatcgatgt gatagattgg ataagaaacg cgggtgttct
    10201 cggtttttga accatcatca aagtcgatag tgccatcttc acgcacggtg acgttttcca
    10261 gcagcgcatc acgacggata gcgttgtaga tttcaggttc cgcttctttc gacagcttga
    10321 tagtttttgc gtagcagccg ccttcgaaat taaacacgcc gtcatcgtcc cagccgtgtt
    10381 cgtcatcgcc aatcaggcgg cgtttcgggt cggtggaaag ggtggtttta ccggtgccgg
    10441 aaaggccgaa gaacaccgca acatcgcctt tctcgccaac gttggcggag cagtgcatag
    10501 aagcgatacc tttcagcggc agcaggtagt tcatcatcga gaacatccct ttcttcattt
    10561 cgccgccgta ccaggtgccg ccaatcagct gcatgcgctc ggtcaggtta aacgccacga
    10621 agttttcgga gttcagaccc tgttctttcc actgcgggtt agtgcacttc gcgccgttca
    10681 taacgataaa gtctggtttg aaacctgcca gctcttcatc gctcgggcga ataaacatgt
    10741 ttttgacaaa atgcgcctgc caggccactt cggtgatgaa acggacggaa agacgagtat
    10801 ccgggttcgc accacagaaa gcgtcgacaa cgaacagacg tttgccggaa agctgcctgg
    10861 tcaccaggcc tttcagatgc tgccaggttt ccggagagag aggtttgttg tcgttcttac
    10921 ctttgccttt gtctgcccac cagaaagtat cgcgagtggt atcgtcacgg acgatatact
    10981 tatcttttgg tgaacgaccg gtgaagatcc cggtatcgac ggcaacggca cccagattag
    11041 ttaacacccc gcgctcataa cctgtcaggc tcggatcgag ctcttcctga tacagcaggt
    11101 cgtagcttgg gttgtaaacg atatcatgta cgtcactgat accataagcc tcgagttctt
    11161 gcgggggcaa accattgtta acgcgcattt cactgctcct tagccaatat gtattgcctg
    11221 aatagtaaag tctttttggg ggtgttaacc gcgacaaggc tcatagattt acgtatctgg
    11281 agaaattcat atttgacaaa aaactccgtg attcctgtca cgaaacggtt gctattatcg
    11341 cagaaatagc attttaggtg tggaaaatgt tctcaaaagg ttaaatcttg gtttatatgg
    11401 cgggaatatt gtgagtggaa tcgcattcct gaatgaaatt gatgcaaatt caaccggtaa
    11461 agtatcgatt caccacgcta actttttttg attattttct gaaaaaagca aaatactact
    11521 gcccggatgc gataattaac ccaccttttg attctggata accaacatgg ataacgttga
    11581 actttcaccg gcaacacgct gggggatgat tgctaccgga ttacttcagg ggatgattgc
    11641 taccggatta cttcaggggc tggtttgcta cctgcttatc gcctggcttg ccggaaaaaa
    11701 tcacagttgg attgtttatg gcgtgcctgc gaccgtcgcg ttttcatccg ttttactctt
    11761 ctccgtgatc tcttttaaac agaagcgcct ttggggatgg ctggcgctgg tgtttattgc
    11821 cacgacaggg atgagcggtt ggctgaagtg gcaaactgat ggcatgaacc cctggagagc
    11881 tgaaaaggcg atttgggatt ttggctgcta tctgctgttg atggcaatgt tgttgctacc
    11941 gtggatacaa caaagccttc gtatccgcaa tggcagtagc cgctatagct acttttacca
    12001 gtcagtatgg cataacgtac ttatattact ggtgattttt ctctccaatg gcctgacgtg
    12061 gctggtgctt ttactgtggg gtgagttgtt taaacttgtt ggcatcacgt ttttcaatac
    12121 gctctttttt gcaaccgact ggtttatgta tcttacattt ggtctggtta ccgcactggc
    12181 ggtgatcctc gcgcgtacac agtcacgttt aatcgactct attcaaaagt tgttcacgct
    12241 aatcgccacg gggttgctgc cgttagtatc attgctaacc ctgatgttta tcatcaccct
    12301 gccgtttacg ggcctgagcg cgatttctcg ccacatctcc gccgccgggt tgctgttaac
    12361 gctggccttt ttgcaattga tcttaatggc tattgtccgc gatccgcaaa aagcgtcact
    12421 tccctggacg gggccgttgc gttgcctgat taaaaccgct ttgctggttg ctccgctgta
    12481 tgtgttcgtc gccacctggg ggttatggct gcgggtcgct cagtacggct ggactgtcga
    12541 ccgcttgcag ggcgcgctgg cggagctggt gttactggtg tggtcgctgg ggtattttgt
    12601 cagtatcgtc tggcgtaatg ggcaaaatcc ccttgttctt cagggcaaag tgaaccttgc
    12661 ggtttcgtta ttggtgttgg tgatactggt gcttcttaat tcgccggtgc tggacagtat
    12721 gcgtattagc gtgaacagtc atatggcgcg ttatcagagc ggcaaaaaca cgccagacca
    12781 ggtaactatc tacatgctcg aacagagcgg tcgctatgga cgtgcggcgc ttgagtcgct
    12841 gaaaagcgat gccgggttta tgaaagaccc gaaacgcgcg cgggatctgc tgatggcgtt
    12901 agatggagag caacatcttc aggaacaggt atcggaaaaa gtattcgccg aaaatgtgtt
    12961 aattgcccct ggttctgtta aaccggatgc gacattctgg tcggccttaa tccaggatcg
    13021 ctataacgtg atgacctgta ttgaaaaaga cgcctgcgta ctggtcgagc aagatctgaa
    13081 tagtgatggt caggcggagc ggatcctgtt tgcttttaat gatgacagag tcattgtcta
    13141 tggctttgac tcagacagaa aagaatggga cgcgcttgat atgagtttac ttccgaacga
    13201 aataacgaaa gaaaaattac tcacagctgc gaaggatgga aaactgggga cgaggccgaa
    13261 agcgtggcgc gatctgacgg tagatggtga aacgctggaa ataaacttaa gtaagtgaca
    13321 acgcttttat cggatacgac gctgtatctg accaaaataa tttgcgattt atttcatagg
    13381 cgctggtaag cgcagcacat caggccatta agtcgtaaaa tccggcagag ccctctctgc
    13441 cggacatact cattaatgaa cttacggatc tgccggagac gcgttgttgc ggatttcagc
    13501 aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca tgtcaatttc
    13561 gccatcttcc gccaggatgc tatcaacttc ttcatcaggc agcgttttca gcgcatcggc
    13621 gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt aaaccgtcac
    13681 ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca gcagttcttc
    13741 ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgtt gggtattttg
    13801 cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta cgtcgccggt
    13861 gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt ctaggcaggc
    13921 cgccagggta tcaccttcca gaccaactac gccctgatag cgttcgcctt cgctcggggt
    13981 aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat tttctggaat
    14041 ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt taataaccgc
    14101 cagattcatc ggaccgtcgc cctgcagctg tacggtgata tcaccatcaa acttcagcgt
    14161 agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa cgggctgcgg
    14221 ataatcgtgg ttctcaagga tctgttgcag ggtttccgaa acggttacca gttcgccgcg
    14281 aacggcaaaa ttttcaaaca gatagcgatg taattggtca tgttgcggca taatcatctc
    14341 tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt cgcggcgctc
    14401 ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt tacgtgccat
    14461 cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca aggctgcctc
    14521 gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt cgtcatttcc
    14581 ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc tgcgctgccc
    14641 gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg ttttataaaa
    14701 acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggtttct ctttcatggc
    14761 gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac ggatggcgtt
    14821 gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag taacgaatac
    14881 caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc gttctttcag
    14941 ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct ttcggataac
    15001 caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagacccgta tgctcaagtt
    15061 ttaccgccag gttgtgcgga tgagcattgg tgagcaaaat tcgctgctta ccgctggctt
    15121 tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcgcgcggt cccatctcgg
    15181 tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga cagtaccagt
    15241 ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct tcctgtggcg
    15301 taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa tagttatcga
    15361 aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg tcctgccagg
    15421 caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga cagggtagca
    15481 taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat cagttgcggg
    15541 ttcaggcagc tttcataata ggcctgaatc tccatcatgc gagtgcgatg acgctggtaa
    15601 cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag tagcagcaat
    15661 gtggtgctga gatagcgcca caggccggaa cgatccggga tcggatgaag gccaatatgc
    15721 tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cttcggcgtt aaacggcgta
    15781 tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg cgggtagtcg
    15841 taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc gctgacaatc
    15901 aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt ttcatgcgtg
    15961 ataaacggcg cggtggaggt tgccaccagg ttatccagcg attcagcact caccgggcgt
    16021 aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag cgcgtcccag
    16081 tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc atctttggca
    16141 gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa taccatgccg
    16201 gatttttgaa tcgctgaacg taatgaggca ctgacgcgag attcatcttc cggtttaggg
    16261 tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag ttcagattct
    16321 ggtaatggta atgagcgggc gtcattccag atgatctgcg agcagtcaaa cggtaaaaaa
    16381 ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat tcccgtaccg
    16441 ctaatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt tacgctggtg
    16501 gcttcaatgg tctgcgcgcc tttcatccat gagagagtga atttcagcgg catatccagc
    16561 gggatccaga ataacagcat aaacagcact agcagcgagc ccgcagcgat aatcgtactg
    16621 cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag atatcgcccc
    16681 tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc gagatcctga
    16741 gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag tgaaatattg
    16801 ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt accgcgcaga
    16861 caatgaattt ctcgcaggga ggattttgcc gggggcgcga atagccccca cagacctgcg
    16921 cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg ggtaatcagg
    16981 cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag cccgcgcgga
    17041 cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc gatttgctca
    17101 ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc atagccgcgc
    17161 gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccaa cggcagcgaa
    17221 tcggtatgaa tcagttcaac ggtattctca tcgttgatgt actgttccca gaaggggggc
    17281 aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc ggtagagatg
    17341 ccgtaacgtg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc attcagcgcc
    17401 agagagatcg gtgccgcgct agcttccgtt gggccaggca cctgtaatac ctgcgtcagg
    17461 ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg cgtttgcgca
    17521 tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt tatcagccat
    17581 cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt gctcatgctt
    17641 tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cggacacaat acatgattct
    17701 gcggttggct atcggcaagc gtggagctta acttgagcat cattaagcac atcacagtaa
    17761 ccgctgatca tagcaaaccc tctgaaggcg gaatatcagc aaattcctgg agttatttca
    17821 acctattgac ttttaatttg atttgaggac gattatttat taagttacat aaatgtaaat
    17881 aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg ctgatttcac
    17941 agcatagatc ccacgatgaa caaatcatta caaaaaccca ccattctgaa tgttgaaact
    18001 gtagcccgtt cccgactgtt taccgtcgag agcgtggatc tggagttcag caatggcgtg
    18061 cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat tgtgccgatt
    18121 gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc ctacgaatta
    18181 ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc taaccgcgag
    18241 ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa gctcagcatg
    18301 gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct ctacccggaa
    18361 tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct ggcgcatatg
    18421 atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc gctgttcctc
    18481 gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg cctgatgcac
    18541 aacgcttatc aggcttatgg ggttcctgca atatattgat ttttcacgat tttgttagcc
    18601 ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc aactgaaaag
    18661 gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc gataatggtc
    18721 gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg
    18781 cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac cgtcacaata
    18841 cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt catataagcg
    18901 tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg atctttaatc
    18961 gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc aatccagacc
    19021 gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact gttagtggta
    19081 ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca gccggtaccc
    19141 tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag gaatgccagc
    19201 ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac taacgcctga
    19261 tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg ggagatagcg
    19321 acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc gtaaatcact
    19381 ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac gccgccctga
    19441 tcgttttcaa ttttgctgat aaaccacggg tccactagga agccgccgtt cgccatgacc
    19501 gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc cagcgattcg
    19561 gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc agcgtagtcg
    19621 acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc cagcccctga
    19681 cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca gtcagaaccg
    19741 gcacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag cgtcagacct
    19801 ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg acgcagtgcc
    19861 tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag cgccataacg
    19921 gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac ttgtgccagc
    19981 caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag aacatcggtc
    20041 actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc ccagcgaacg
    20101 ccttccatac tcaatgcgac ggtcgacccg tccgccagca tcgccgtcgc tccctgagga
    20161 ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc cttcagcgta
    20221 tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac atttgccggg
    20281 ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc ctgctgcgcg
    20341 gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc ataggcactt
    20401 tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg cgcagagaaa
    20461 gcaatctccg gcgcgtgata attagcgtta atcgcctcag tgcgtgtctg atcgaactgt
    20521 tgctgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg ccgcgcgacg
    20581 gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcaa cccggctatc
    20641 accgccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac ttgtgccgca
    20701 gcaccgacac cataggcgcg gtaaccaagg taaatcttgt tcagataaag ctcgaggatt
    20761 tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt aatcttacgc
    20821 atcatcgtgc gttctggact gaggaagaaa tttctcgcca gctgctgggt aatggtactc
    20881 gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa gatccccacc
    20941 gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc
    21001 atctccggtg ggatttgatc caacgtaacc ggaatacgac gtttctcacc gtattgagca
    21061 atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac atcttttaat
    21121 gtcaccacat ccggcagttg tggctcgatg tagcggtata ggccataaat cgagcctgct
    21181 cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt cactggaaat
    21241 ttcccattta gtttcatttg ggcagtttat aaacaaacgc gcggtagtat aaaggcaagc
    21301 cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca caatgacatt
    21361 taagatctgg caaattggtt tgcatttaca acagcaagaa gcggcagcgg ttgcgatcgt
    21421 gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg cgcacgacat
    21481 tatcaaagat gggcggattg ttgatgcgca gcagctggct aaaacgttgt taccgtggag
    21541 tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc gcacattaca
    21601 gcggtcattt ccgcgcccgt cgatgtccct tggtgagcgg gagcaaacgg cctggctgtc
    21661 agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgcttcg attacagcga
    21721 agactcactc agccccgctt ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct
    21781 gcttacgctg gcagaaaggt tgcgtgttca tgttagtgcg atcaccccgg atgccagtgc
    21841 attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa
    21901 cgaacagtgg ctgtgggcga cgcgctatag ctgggggcgc aaactggcgg tggggatgac
    21961 tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gacagcgtcg cgatatgtgg
    22021 cgaaggcgga tttgacccct gggaggccgt ttctgttcgt cagccgccgc taccgccgcc
    22081 tggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact gatgaatccg
    22141 ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttctgcg tttctggttg
    22201 ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact gcgtctgaca
    22261 ggcagcgccg aagcgcgcat agacgcagtt ttgcttcagg cggaacaaca actcgcccgc
    22321 agcttacaga taacgaagcc acgtttgctg gagcaacagc agttacgcga acagcgttcg
    22381 caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga agcactggcg
    22441 gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg aacgctggag
    22501 atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc actccgccag
    22561 gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca gggacgctgg
    22621 caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg actggtggtt
    22681 cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga tgttagttac
    22741 gctcattttt ctatcatcga cacaccatga agagcgcgac gcattaattc gactacgggc
    22801 aagtcatcac cagcagtggg ccgcactgta tcgcctggta gacaccactc ccttcagcga
    22861 ggaaaaaacg ctgccctttt cgccactgga ttttcagtta tccggcgcgc aactggtttc
    22921 ctggcatcca tccgcgcagg gaggtgagtt ggcgttgaaa acgctgtggg aagcagtgcc
    22981 gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt cgttaagcgt
    23041 ggaaggtgat gatcttttgt tcacgctaca actggagatg ccgcatgagg gttaaacgct
    23101 ggttgttggc aggtattgca ttgtgccttt taaccggtat gcgtgaccct tttaaatcgc
    23161 cggaagatct atatgccgga ttagcgaact tagccagtgg cgctatcagg ggatggtagg
    23221 gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa aagaaatggc gacgggtgca
    23281 gcaaaacgat gtgctggaaa acggctggac aattttacag ctgacgccag acgtactaac
    23341 gctgggtacc gggacaaact gcgaaccgcc acaatggttg tggcaacggc aaggagatac
    23401 aaatgaagca atggatagcc gcactactgt tgatgctgat acccggcgta caggcggcaa
    23461 agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg ttgcaggcgc
    23521 tggctgaaca ggagaagttg aacctggtcg tgtcgccaga cgtcagcggt acggtgtcgt
    23581 tacatctaac agatgttccc tggaagcagg cactacaaac tgtagtgaaa agcgccggac
    23641 tgataacgcg gcaggaaggc aacattctct cagtgcattc cattgcctgg cagaatgaca
    23701 atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc aaatctgccg ctggaaaatc
    23761 gcagtataac cctgcaatac gccgacgcgg gagaactggc gaaagcgggg gagaagctac
    23821 tgagtgccaa agggagtatg accgtcgata aacgcaccaa tcgccttttg ctacgagata
    23881 acaaaacggc gttaagcgcg cttgaacagt gggtagcgca aatggatctg ccggtcgggc
    23941 aggttgagct gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt gagttaggcg
    24001 tgaaatggac gctggccgat gcgcaacacg ctggtggcgt tgggcaagtc accacgcttg
    24061 gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg ttttaacatt gggcgcatca
    24121 acggacgctt gctggatctt gagctttccg cgctcgaaca aaaacagcag ctggatatta
    24181 tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa caggagagcg
    24241 aaattccata tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa tttaaagagg
    24301 ccgtcctggg gatggaggtc acgcccacgg tgttacaaaa aggtcgcatc cggctgaaat
    24361 tacacatcag ccagaacgtt ccggggcagg tgctacagca ggccgatggc gaagtgctgg
    24421 cgattgataa gcaggagatc gaaacgcagg tcgaggtaaa aagcggagaa acgttggcgc
    24481 tgggcggcat ttttacccgt aaaaataaat cgggtcagga tagcgtaccg ttgcttggcg
    24541 acattccctg gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa cgacgcgagt
    24601 tagtggtgtt tatcacgcca cgactggttt ccagtgagta aacagccgta aaagcggtaa
    24661 tgtttttatg ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag catacaagga
    24721 gtaccgattt gagagttggt gctcttcgct gcctgcgttc catgatgatg atttatcatt
    24781 caggcggcat tttgctgtct tttttacgct aatcttaccc ggtgatttat cgccagagcg
    24841 gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca gagggatggg tgatttattc
    24901 agttgccaaa cccgctggag tattgagata attttcagtc tgactctcgc aatatcttat
    24961 gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag cgggtttatc attaacgaat
    25021 agtcttagta gtaccgaaaa aatggcagag aaacgcaata tctttctggt tgggcctatg
    25081 ggtgccggaa aaagcactat tgggcgccag ttagctcaac aactcaatat ggaattttac
    25141 gattccgatc aagagattga gaaacgaacc ggagctgatg tgggctgggt tttcgattta
    25201 gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt gaccgagaaa
    25261 cagggtattg tgctggctac tggcggcggc tctgtgaaat cccgtgaaac gcgtaaccgt
    25321 ctttccgctc gtggcgttgt cgtttatctt gaaacgacca tcgaaaagca acttgcacgc
    25381 acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa caccgccgcg tgaagttctg
    25441 gaagcgttgg ccaatgaacg caatccgctg tatgaagaga ttgccgacgt gaccattcgt
    25501 actgatgatc aaagcgctaa agtggttgca aaccagatta ttcacatgct ggaaagcaac
    25561 taattctggc tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga tgtcgcgtta
    25621 tggagaggat tgtcgttact ctcggggaac gtagttaccc aattaccatc gcatctggtt
    25681 tgtttaatga accagcttca ttcttaccgc tgaaatcggg cgagcaggtc atgttggtca
    25741 ccagcgaaac cctggctcct ctgtatctcg ataaggtccg cggcgtactt gaacaggcgg
    25801 gtgttaacgt cgatagcgtt atcctccctg acggcgagca gtataaaagc ctagctgtac
    25861 tcgataccgt ctttacggcg ttgttacaaa agccgcatgg tcgcgatact acgctggtgg
    25921 cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc ggcggcgagt tatcagcgcg
    25981 gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca ggtcgattcc tccgttggcg
    26041 gcaaaactgc ggtcaaccat cccctcggta aaaacatgat tggcgcgttc taccagcctg
    26101 cttcagtggt ggtggatctc gactgtctga aaacgcttcc cccgcgtgag ttagcgtcgg
    26161 ggctggcaga agtcatcaaa tacggcatta ttcttgacgg tgcgtttttt aactggctgg
    26221 aagagaatct ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac tgtattcgcc
    26281 gttgttgtga actgaaggca gaagttgtcg ccgccgacga gcgcgaaacc gggttacgtg
    26341 ctttactgaa tctgggacac acctttggtc atgccattga agctgaaatg gggtatggca
    26401 attggttaca tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg acgtcggaac
    26461 gtctcgggca gtttagttct gccgaaacgc agcgtattat aaccctgctc acgcgggctg
    26521 ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc gtatttaccg catatgctgc
    26581 gtgacaagaa agtccttgcg ggagagatgc gcttaattct tccgttggca attggtaaga
    26641 gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc gattgtcaat
    26701 cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg tctattagct tcaggttaat
    26761 tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa tggatgaatt caaaccagaa
    26821 gacgagctga aacccgatcc cagcgatcgt cgtactggtc gttctcgtca atcttctgaa
    26881 cgttctgagc gtactgaacg tggcgaaccg cagatcaatt ttgatgatat tgaacttgat
    26941 gacactgacg atcgccgtcc gactcgtgcg caaaaagagc gcaatgagga accggaaatc
    27001 gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt agagcgtcgt
    27061 ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat gatgatgggc
    27121 gtcggcattc tggttctact gctgttgatc atcggtatcg gttctgcgct aaaagccccc
    27181 tcgaccactt ccagcgatca aaccgcgtct ggcgagaaga gtattgatct tgctggcaat
    27241 gcgaccgatc aggcgaatgg cgtgcagcca gcgccgggaa ccacgtctgc ggaaaatact
    27301 cagcaggatg tttctctgcc accgatctct tctacgccga ctcaagggca aaccccggtg
    27361 gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc gctgacccag
    27421 ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt ccacgttgcc gactgaaccc
    27481 gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg acacggcgaa aacgcaaacc
    27541 gctgaacgtc cgtccactac gcgcccagtt cgtcagcagg cggtgattga accgaaaaaa
    27601 ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac agacgccgaa gcgtactgaa
    27661 cctgaaccag ctgcccctgt ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca
    27721 ccaaaagaga cggcgactac ggctccagta cagacggcat ccccggcgca aaccacggca
    27781 acaccagccg ctggagggaa gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc
    27841 agccattaca ctctgcagct gagcagttcc tctaactacg acaacctgaa cggttgggcg
    27901 aagaaagaga atctgaaaaa ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg
    27961 tatgtcctgg tttctggcgt gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca
    28021 ttgccagcag atgttcaggc caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc
    28081 gatctgaagt aatcaaggtt atctcccgca atggtttatc gttgcgggag ctgcctgaag
    28141 cgctggatgc tgtcggagct ttctccacag ccggagaagg tgtaattagt taatcggcat
    28201 gaagaaaaat cgcgcttttt tgaagtgggc agggggcaag tatcccctgc ttgatgatat
    28261 taaacggcat ttgcccaagg gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt
    28321 gtttctcaac accgactttt ctcgttatat ccttgccgat atcaatagcg acctgatcag
    28381 tctctataac attgtgaaga tgcgtactga tgagtacgta caggccgcac gcgagctgtt
    28441 tgttcccgaa acaaattgcg ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag
    28501 ccaggatccg ttccgtcggg cggtactgtt tttatatttg aaccgctacg gttacaacgg
    28561 cctgtgtcgt tacaatctgc gcggtgagtt taacgtgccg tttggccgct acaaaaaacc
    28621 ctatttcccg gaagcagagt tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta
    28681 ttgtgagtct tacgccgata gcatggagcg cgcagatgat gcatccgtcg tctattgcga
    28741 tccgccttat gcaccgctgt ctgcgaccgc caactttacg gcgtatcaca caaacagttt
    28801 tacgcttgaa caacaagcgc atctggcgga gatcgccgaa ggtctggttg agcgccatat
    28861 tccagtgctg atctccaatc acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa
    28921 attgcatgtc gtcaaagttc gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt
    28981 ggacgaactg ctggctttgt acaaaccagg agtcgtttca cccgcgaaaa aataattctc
    29041 aaggagaagc ggatgaaaca gtatttgatt gccccctcaa ttctgtcggc tgattttgcc
    29101 cgcctgggtg aagataccgc aaaagccctg gcagctggcg ctgatgtcgt gcattttgac
    29161 gtcatggata accactatgt tcccaatctg acgattgggc caatggtgct gaaatccttg
    29221 cgtaactacg gcattaccgc ccctatcgac gtacatctga tggtgaaacc cgtcgatcgc
    29281 attgtgcctg atttcgctgc cgctggtgcc agcatcatta cctttcatcc agaagcctcc
    29341 gagcatgttg accgcacgct gcaactgatt aaagaaaatg gctgtaaagc gggtctggta
    29401 tttaacccgg cgacacctct gagctatctg gattacgtga tggataagct ggatgtgatc
    29461 ctgctgatgt ccgtcaaccc tggtttcggc ggtcagtctt tcattcctca aacactggat
    29521 aaactgcgcg aagtacgtcg ccgtatcgac gagtctggct ttgacattcg tctggaagtg
    29581 gacggtggcg tgaaggtgaa caacattggc gaaatcgctg cggcgggcgc ggatatgttc
    29641 gtcgccggtt cggcaatctt cgaccagcca gactataaaa aagtcattga tgaaatgcgc
    29701 agtgaactgg caaaggtaag tcatgaataa gtttgaagat attcgcggcg tcgcttttga
    29761 tcttgatggt acgctggtcg acagtgctcc tggtcttgct gctgcggtag atatggcgct
    29821 gtatgcgctg gagttgcccg tcgcaggtga agaacgcgtt attacctgga ttggtaacgg
    29881 cgcagatgtt ctgatggagc gcgcattgac ctgggcgcgt caggaacgtg cgactctgcg
    29941 taaaacaatg gggaaaccgc ccgttgatga cgacattccg gcagaagaac aggtacgtat
    30001 tctgcgtaaa ctgttcgatc gctactatgg cgaggttgcc gaagagggga cgtttttgtt
    30061 cccgcacgtt gccgatacgt tgggcgcgtt gcaggctaaa ggcctgccgc taggcttggt
    30121 caccaacaaa ccgacgccgt tcgtcgcgcc gctgctcgaa gccttagata ttgccaaata
    30181 ctttagcgtg gttatcggcg gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct
    30241 gttactggtg gctgagcgga tgggaattgc cccacaacag atgctgtttg tcggcgactc
    30301 acggaatgat attcaggcgg caaaagcggc aggttgccca tcagttggct taacctacgg
    30361 atacaactac ggcgaggcta tcgatctcag ccagcctgat gtaatttatc agtctataaa
    30421 tgaccttctg cccgcattag ggcttccgca tagcgaaaat caggaatcga aaaatgacta
    30481 agcccatcgt ttttagtggc gcacagccct caggtgaatt gaccattggt aactacatgg
    30541 gtgcgctgcg tcagtgggta aacatgcagg atgactacca ttgcatttac tgtatcgttg
    30601 accaacacgc gatcaccgtg cgccaggatg cacagaagct gcgtaaagcg acgttggata
    30661 cgctggcctt gtatctggct tgtggtatcg atcctgagaa aagcaccatt tttgttcagt
    30721 cccacgtacc agaacatgcg cagttaggct gggcactgaa ctgctatacc tacttcggcg
    30781 aactgagtcg catgacgcag tttaaagata aatctgcgcg ttatgccgag aacatcaacg
    30841 ctggtctgtt tggctatccg gtgctgatgg ctgcggacat cctgctgtat caaactaatc
    30901 tggtaccggt gggtgaagac cagaaacagc acctggaact gagtcgcgat atcgcccagc
    30961 gtttcaacgc gctgtatggc gagatcttta aggtgccgga gccgtttatt ccgaaatctg
    31021 gcgcgcgcgt aatgtcgctg ctggagccga ccaagaagat gtccaagtct gacgataacc
    31081 gcaataacgt tatcggcctg ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg
    31141 cggtcactga ctccgacgag ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg
    31201 tttccaacct gctggatatc ctttctgcgg taacgggcca gagcatccca gaactggaaa
    31261 aacagttcga aggcaagatg tatggtcatc tgaaaggtga agtggctgat gccgtttccg
    31321 gtatgctgac tgaattgcag gaacgctatc accgtttccg caacgatgaa gccttcctgc
    31381 aacaggtgat gaaagacggc gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag
    31441 cggtgtacga agcgattggt tttgtggcga agccgtaata tcaaaacgct cggacggtcc
    31501 cctcgcccct ttggggagag ggttagggtg aggggagcag gcaagcgcca gtgggaatta
    31561 ccctttgctc gcattgtcga ataaatcact tcgtcatcac tctgaaaccg gggaaacccg
    31621 gtttttttat cctcttttcg caacaacccg aaaaaatgtg aagcgcctcg ccgtttccac
    31681 atctcgtcgt cgccacttat cttcatttta atgaagatat aaatattcat tttattgaaa
    31741 atatattatg cgtcgaacgt ttatcaaaaa agaaggcgtc gtcatcacga cactggcccg
    31801 ttatttgttg ggtgaaaaat gcggtaatcg actgaaaacc atagatgagc tggcaactga
    31861 atgccgttca tcggttggcc tgacgcaggc cgcgttgaaa acgctggaat caagcggagc
    31921 gatacggatt gaacgccgtg ggcgcaatgg cagttatctt gtcgagatgg ataacaaagc
    31981 attgctgagt catgtggata tcaacaacgt ggtatgtgcg atgcctttgc cctatacccg
    32041 cttgtacgaa ggtttagcga gcggattgaa agcccagttt gatggcattc ctttttacta
    32101 tgcgcatatg cgcggcgcgg atattcgcgt ggagtgttta cttaacggtg tctatgacat
    32161 ggcagtagtt tcccgcctgg cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc
    32221 cctcgagctt ggcccgcata cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga
    32281 gtctggaaat gttaagcgag taggtctcga tagtcgttcg gcggatcaga aaatcatgac
    32341 cgatgtcttt tttggcgata gtgatgtgga acgagtcgat ctctcttatc acgagagttt
    32401 acaacgcatt gttaaaggcg atgtcgatgc ggtgatctgg aacgtggtgg cggaaaacga
    32461 actgaccatg ctgggattag aggcgacgcc gctaacagac gatccgcgat ttttacaggc
    32521 caccgaagcc gtggtcctga cgcgagtcga tgattaccca atgcaacaat tgctgcgtgc
    32581 tgttgtagat aaacacgccc tgcttgccca tcaacaacgg gtggtgagtg gggaacagga
    32641 acccagttat taaacgaaag gcatctgata tggaaaccag actcaacctg ctttgcgagg
    32701 caggcgttat tgataaggac gtctgcaaag gcatgatgca ggtcgtcaac gtactggaaa
    32761 aagagtgcca tctgccggtg cgcagtgagc aaggaacgat ggcgatgaca catatggcga
    32821 gtgcactgat gcgcagtcgc cgtggtgaag aaatagagcc gctggataac gagttgctgg
    32881 cggaactggc gcaatccagc cactggcaag ccgttgtgca attgcatcag gagttgttga
    32941 aggaattcgc tctggaagtt aacccgtgtg aagaaggcta ttttctggcc aatctttatg
    33001 gattatggat ggctgctaac gaagaggttt gaagctcccg acaaatccac gtcgcacaac
    33061 ctaaatattc aaaaaaatga atatttaatt caaggaacag aaacgaggca acaagatgtt
    33121 tgtagaagca ttgaaacgcc agaacccggc gctgatttcc gccgcactaa gtttgtggca
    33181 gcagggcaaa atcgccccgg acagctgggt gatcgacgtg gatcagatac tggaaaacgg
    33241 taagcggctc attgagacgg cgcggcttta cggcattgaa ctgtatctga tgaccaagca
    33301 atttggtcgt aatccgtggc tggcggaaaa attgctggca ttaggctaca gcggcattgt
    33361 ggcggtggat tacaaagagg cgcgagtcat gcgccgcgct ggtttgccag tggcgcatca
    33421 ggggcatctg gtacaaatcc cttgtcatca ggttgctgac gccgttgaac agggcaccga
    33481 catcatcacc gtgtttactc tcgacaaagc gcgggaagtt tctgcggcag cggttaaggc
    33541 cggacgggtc cagcctgtgc tgctcaaagt ttatagcgac gatgattttc tttatccggg
    33601 gcaggagagc ggttttgtac agcattcgct gcacgaggtg attgctgaaa ttaagaaact
    33661 gccggggctg catttagccg gactaaccca tttcccttgc ctgctttggg atgaggctgc
    33721 tgggaaagtt ttgccaacac cgaatcttca cacgctggta caagcacggg atcaactggc
    33781 gaaatctggt attgcgattg agcaactgaa cgtaccttca gcgaccagct gcacttcgct
    33841 gccattgctg gcggaatacg gtgcgactca tgccgaaccc ggtcatgcgc tgaccggtac
    33901 tattccggca aaccagcagg gcgatcagcc tgaacgtatc gcgatgctct ggttaagtga
    33961 aatctcccat catttccgtg gcgacagcta ctgctacggc ggcggttact accgtcgtgg
    34021 tcatgcgcaa catgcgctgg tgtttacgcc agaaaatcaa aagattactg aaaccaatct
    34081 caaaactgtg gatgacagca gtatcgacta caccctgccg ctggcaggcg agtatccggt
    34141 gagcagtgca gtggtgctct gttttcgcac gcagattttt gtcacccgta gcgatgtggt
    34201 gctggtgtcc ggtattcagc gcggcgaacc agaaatagtt ggtcgttatg acagcctcgg
    34261 caaccctctg gaggcataaa tggcgcgatt tgtggtgtta gtgattgata gctttggcgt
    34321 aggggcaatg aaagatgtca cgctggtgcg cccgcaagat gccggggcga atacctgtgg
    34381 tcacatcctg agccagttgc cgcatttgca gctaccaacg ctggagaagc tggggctaat
    34441 caacacattg ggttatgcgc caggcgatat gcagccgtca gattccgcaa cctggggcgt
    34501 ggcagagctg caacatgaag gtggcgatac ctttatgggg catcaggaaa ttttaggcac
    34561 gcgcccgtta ccgccgctgc ggatgccttt tcgcgatgtg attgaccgtg ttgagcaggc
    34621 attagtttcc gctggctggc aggtggagcg ccgtggcgat gatctgcaat ttctgtcggt
    34681 caatcaggcg gttgcgattg gcgataatct cgaggcggat ttaggccaag tctataacat
    34741 taccgccaat ctctctgtga tctcttttga cgacgcaatc aaaattggtc gtatcgtgcg
    34801 tgaccaggta caggtcggtc gggtcattac atttggcggc ctgttaacca acagtcagcg
    34861 tattctcgat gccgcagaaa gcaaagaagg gcgctttatt ggtattaatg cgccgcgttc
    34921 tggcgcttat gacaacggct tccaggtcgt gcatatgggc tacggtgttg atgaaaaagt
    34981 gcaggtgccg caaaaactgt atgaagcggg cgtgccaacc gtgctggtgg gtaaggtggc
    35041 agatatcgtc agcaatcctt atggtgtgag ctggcaaaac ctggtggata gccagcggat
    35101 tatggatatc accctcgacg aatttaacac ccatccgacg gcgtttattt gcatcaatat
    35161 ccaggaaacc gacctcgctg gtcatgcaga agacgtcgca cgttatgccg aacgtttgca
    35221 ggtcgttgac cgtaaccttg cccggcttgt tgaggcgatg cagccagatg attgcctggt
    35281 cgtgatggcg gatcacggta acgatccgac cattggtcac agccaccata cccgtgaagt
    35341 ggtgccagtg ctggtttatc agcaagggct ggtcgccacg cagctcggtg tgcgcaccac
    35401 gctttctgat gtgggagcta ccgtgtgtga atttttccgt gcgccaccgc cacaaaatgg
    35461 tcgctctttt ctttcctccc tccggtttgc aggagacacc ctatgagttt tgatccgacg
    35521 ggttacaccc tggcccatga gcatctgcat attgatctct ccggctttaa aaacaacgtg
    35581 gactgccgcc ttgatcagta tgcgttcatt tgccaggaga tgaacgacct gatgacccgg
    35641 ggtgtacgta atgtgattga gatgaccaac cgttacatgg ggcgcaatgc gcaatttatg
    35701 ctcgatgtaa tgcgcgaaac cgggatcaac gtggtggcct gtactggtta ttaccaggat
    35761 gcgtttttcc cggaacatgt ggtgacccgc agcgtgcagg aactggcgca ggagatggtc
    35821 gatgaaattg aacagggtat cgatggcacg gagctgaaag ccgggatcat cgcggagatc
    35881 ggctccagcg aaggaaagat tacgccgctg gaagagaagg tatttattgc cgctgcgctg
    35941 gcgcataacc agaccggacg cccgatctcc acgcatacgt cgttcagcac catggggctg
    36001 gaacaactgg ctctgttgca agcccacgga gttgatcttt cgcgcgtcac cgttggtcac
    36061 tgcgatctga aagacaacct cgacaacatt ttgaagatga tcgatctcgg cgcgtacgtg
    36121 cagttcgaca ccattggtaa gaacagttac tacccggacg aaaagcgcat tgcgatgctc
    36181 cacgcgctac gtgaccgtgg gttgctgaac cgcgtcatgc tgtcgatgga tattacgcgc
    36241 cgctcccatt taaaagccaa tggtggttat ggctatgact atttattgac cacctttatt
    36301 ccgcaattgc gccagtcagg attcagtcag gccgatgtgg atgtgatgtt acgtgaaaat
    36361 ccctctcaat ttttccaata aggacagact catgaaaaag attggcgttg caggcttaca
    36421 gcgtgagcag attaaaaaaa ccattgaagc gacggctcct ggctgttttg aagttttcat
    36481 tcacaacgac atggaagcgg caatgaaggt gaaatccggg caactagatt attacattgg
    36541 cgcgtgtaat accggtgcgg gcgcggcatt gtcgattgcc atcgcggtga ttggctataa
    36601 caaaagttgc accattgcca aaccaggcat taaagcgaaa gacgagcata tcgccaaaat
    36661 gatcgctgaa gggaaagtgg cgtttggcct ttccgttgag cacgtcgaac acgcgattcc
    36721 gatgttgatt aaccatctga aataaaaggc acgactatgg atctgtatat tcagattatc
    36781 gtggtggcgt gcctgacggg tatgacatcg cttctggcgc atcgctcggc ggctgttttt
    36841 catgacggca tccacccgat cctgccgcaa ctgattgaag gctatatgaa ccgtcgcgag
    36901 gcggggagta tcgcttttgg tctgagcatt ggttttgtgg cctcggtggg gatctctttt
    36961 accctgaaaa ccgggctgct caacgcatgg ttactctttc ttcctaccga tatcctcggc
    37021 gtactggcga taaacagcct gatggcgttt ggtcttggcg ctatctgggg cgtgttgatc
    37081 cttacttgcc tgttgccagt taaccagctg ctgaccgcgc tgccggtgga tgtattaggt
    37141 agcctcgggg aattaagctc gccggtggtt tctgcttttg cactgttccc gctggtggcg
    37201 attttctacc agtttggctg gaagcaaagt ctggtcgccg ccgtggttgt tctgatgacc
    37261 cgtgtggtag tcgtgcgcta tttcccacat cttaaccctg aatccatcga aatctttatt
    37321 ggcatggtga tgctgctggg gatcgcgata actcacgacc tgcgtcatcg tgatgaaaat
    37381 gacatcgatg ccagcgggct ttcggtgttt gaagagcgca cgtcgcggat tatcaaaaac
    37441 ttaccttata tcgccatcgt gggagcattg attgccgccg ttgccagtat gaagattttt
    37501 gctggtagcg aagtttcgat cttcacactg gagaaagcgt attccgcagg cgtgacgccg
    37561 gaacaatcgc aaacgctgat taatcaggcg gctctggcag aatttatgcg cggactgggg
    37621 tttgtgccgt tgattgccac caccgcctta gcaacgggtg tgtatgcagt tgcgggcttt
    37681 acctttgttt atgcggtggg ctatctctcg ccgaatccga tggttgcagc ggtattaggc
    37741 gcagtggtta tttcggcgga agtcttgctg cttcgttcga tcggcaaatg gctgggacgc
    37801 tacccgtcgg tgcgtaatgc gtcggataac atccgtaacg ccatgaatat gctgatggaa
    37861 gtggcgctgc tggtcggttc gattttcgca gcaattaaga tggcgggtta taccggattc
    37921 tctatcgcgg ttgccattta cttcctcaac gaatccctgg gccgtccggt acagaaaatg
    37981 gcggcaccgg tcgtggcagt aatgatcacc ggtattctgc tgaatgttct ttactggctt
    38041 ggcctgttcg ttccggctta aggaaggcac ctatgaagac gtttcctttg caaagcctga
    38101 ctcttgctga agcgcagcaa aagcagtttg cgctggtgga cactatttgc cgccattttc
    38161 caggcagcga gtttcttgct ggcggtgatt tagggttaac tccaagtctg aatcaaccgc
    38221 gtattaccca acgggtggag caggtgctgg ctgatgcatt tcacgcacag gccgcggcgc
    38281 tggtgcaggg cgcggggact ggcgcgattc gcgccgggct ggcggctttg ctcaaaccgg
    38341 ggcagcgtct tctggtgcat gacgcgcctg tttacccgac gacacgggtt attattgagc
    38401 agatggggct gacgcttatt actgctgatt tcaatgacct gtcggcactg aagcaggtcg
    38461 tcgacgagca acaacctgat gccgcgctgg tgcagcatac gcgccagcag ccgcaggaca
    38521 gctatgtgct ggcagatgtg ctggcaacgt tgcgcgcggc aggtgttcca gcgttaaccg
    38581 atgataacta tgcggtgatg aaggtagcgc gaatcggctg tgaatgcggc gcgaatgtct
    38641 cgacattctc ctgcttcaag ctgtttgggc cggagggcgt tggtgcagtg gttggcgatg
    38701 ccgatgttat tagtcggatt cgcgccacgc tttactccgg cggtagccag atccagggct
    38761 cacaggcgct ggaagtcttg cgtggtctgg tgttcgcgcc agtgatgcat gcggtgcagg
    38821 cgggagtatc cgaacggttg ctggctttgc ttaacggcgg cgcggtggcg gaagtgaaaa
    38881 gcgccgtcat tgccaatgcg cagtcgaagg tgttgattgt cgagtttcat cagctgattg
    38941 ccgccagggt gctggaagaa gcgcaaaagc gtggcgcgtt gccttacccg gtcggtgcag
    39001 agtcgaaata tgaaatcccg ccgctcttct atcgactttc aggaacgttt cgccaggcga
    39061 atccacaact cgaacattgt gcgattcgca ttaacccgaa tcgcagcggt gaagagacgg
    39121 tgctgcggat tttgcgtgag agtattgcga gtat
//
LOCUS       DAJVPT010000027        37885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVPT010000027 DAJVPT010000000
VERSION     DAJVPT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            384..2087
                     /gene="uidA"
                     /locus_tag="OQM47_001356"
     CDS             384..2087
                     /gene="uidA"
                     /locus_tag="OQM47_001356"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1479574.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            2093..3118
                     /gene="uidB"
                     /locus_tag="OQM47_001357"
                     /pseudo
     CDS             2093..3118
                     /gene="uidB"
                     /locus_tag="OQM47_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            3157..4422
                     /gene="uidC"
                     /locus_tag="OQM47_001358"
     CDS             3157..4422
                     /gene="uidC"
                     /locus_tag="OQM47_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1479575.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(4648..6156)
                     /locus_tag="OQM47_001359"
     CDS             complement(4648..6156)
                     /locus_tag="OQM47_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1479576.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(6257..7432)
                     /gene="manA"
                     /locus_tag="OQM47_001360"
     CDS             complement(6257..7432)
                     /gene="manA"
                     /locus_tag="OQM47_001360"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1479577.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7631..9277
                     /gene="fumB"
                     /locus_tag="OQM47_001361"
     CDS             7631..9277
                     /gene="fumB"
                     /locus_tag="OQM47_001361"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1479578.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9420..10823
                     /gene="fumC"
                     /locus_tag="OQM47_001362"
     CDS             9420..10823
                     /gene="fumC"
                     /locus_tag="OQM47_001362"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1479579.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(10820..11749)
                     /gene="tus"
                     /locus_tag="OQM47_001363"
     CDS             complement(10820..11749)
                     /gene="tus"
                     /locus_tag="OQM47_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1479580.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(11825..13126)
                     /gene="rstB"
                     /locus_tag="OQM47_001364"
     CDS             complement(11825..13126)
                     /gene="rstB"
                     /locus_tag="OQM47_001364"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1479581.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(13305..14624)
                     /locus_tag="OQM47_001365"
     CDS             complement(13305..14624)
                     /locus_tag="OQM47_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCS1479582.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(14849..15592)
                     /gene="rstA"
                     /locus_tag="OQM47_001366"
     CDS             complement(14849..15592)
                     /gene="rstA"
                     /locus_tag="OQM47_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1479583.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            15721..16056
                     /locus_tag="OQM47_001367"
     CDS             15721..16056
                     /locus_tag="OQM47_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1479584.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(16053..16775)
                     /gene="folM"
                     /locus_tag="OQM47_001368"
     CDS             complement(16053..16775)
                     /gene="folM"
                     /locus_tag="OQM47_001368"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1479585.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(16812..18194)
                     /locus_tag="OQM47_001369"
     CDS             complement(16812..18194)
                     /locus_tag="OQM47_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1479586.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(18380..19324)
                     /locus_tag="OQM47_001370"
     CDS             complement(18380..19324)
                     /locus_tag="OQM47_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1479587.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            19848..21380
                     /gene="pntA"
                     /locus_tag="OQM47_001371"
     CDS             19848..21380
                     /gene="pntA"
                     /locus_tag="OQM47_001371"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1479588.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21391..22779
                     /gene="pntB"
                     /locus_tag="OQM47_001372"
     CDS             21391..22779
                     /gene="pntB"
                     /locus_tag="OQM47_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1479589.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(22804..23838)
                     /gene="tqsA"
                     /locus_tag="OQM47_001373"
     CDS             complement(22804..23838)
                     /gene="tqsA"
                     /locus_tag="OQM47_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1479590.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24249..24614
                     /gene="mdtJ"
                     /locus_tag="OQM47_001374"
     CDS             24249..24614
                     /gene="mdtJ"
                     /locus_tag="OQM47_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1479591.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            24601..24930
                     /gene="mdtI"
                     /locus_tag="OQM47_001375"
     CDS             24601..24930
                     /gene="mdtI"
                     /locus_tag="OQM47_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1479592.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(24969..25790)
                     /locus_tag="OQM47_001376"
     CDS             complement(24969..25790)
                     /locus_tag="OQM47_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1479593.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(25890..25973)
                     /locus_tag="OQM47_001377"
     CDS             complement(25890..25973)
                     /locus_tag="OQM47_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479594.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(26066..26401)
                     /locus_tag="OQM47_001378"
     CDS             complement(26066..26401)
                     /locus_tag="OQM47_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1479595.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(26798..28051)
                     /locus_tag="OQM47_001379"
                     /pseudo
     CDS             complement(26798..28051)
                     /locus_tag="OQM47_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            28158..29078
                     /locus_tag="OQM47_001380"
     CDS             28158..29078
                     /locus_tag="OQM47_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1479596.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            29213..30433
                     /gene="mlc"
                     /locus_tag="OQM47_001381"
                     /gene_synonym="dgsA"
     CDS             29213..30433
                     /gene="mlc"
                     /locus_tag="OQM47_001381"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1479597.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            30558..31253
                     /gene="bioD"
                     /locus_tag="OQM47_001382"
     CDS             30558..31253
                     /gene="bioD"
                     /locus_tag="OQM47_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1479598.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(31206..32498)
                     /gene="clcB"
                     /locus_tag="OQM47_001383"
     CDS             complement(31206..32498)
                     /gene="clcB"
                     /locus_tag="OQM47_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1479599.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(32657..33271)
                     /gene="dmsD"
                     /locus_tag="OQM47_001384"
     CDS             complement(32657..33271)
                     /gene="dmsD"
                     /locus_tag="OQM47_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1479600.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(33314..34168)
                     /locus_tag="OQM47_001385"
     CDS             complement(33314..34168)
                     /locus_tag="OQM47_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1479601.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(34170..34787)
                     /gene="dmsB"
                     /locus_tag="OQM47_001386"
     CDS             complement(34170..34787)
                     /gene="dmsB"
                     /locus_tag="OQM47_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1479602.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(34798..36836)
                     /locus_tag="OQM47_001387"
                     /pseudo
     CDS             complement(34798..36836)
                     /locus_tag="OQM47_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(36897..>37885)
                     /locus_tag="OQM47_001388"
     CDS             complement(36897..>37885)
                     /locus_tag="OQM47_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1479603.1"
                     /translation="SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVE
                     QEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQE
                     QWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
                     KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRT
                     HSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPG
                     VSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9614 a         9176 c         9709 g         9386 t
ORIGIN      
        1 ctcatttcgc gggctggtga taactgtgcc cgcgtttcat atcgtaattt ctctgtgcaa
       61 aaattatcct ccccgtgttc ggagaattcc cccccaaaat attcaccgta gccatatgtc
      121 atgagagttt atcgttccca acactctcga acgaacgttc gattgcttat tttatggctt
      181 ctgtcaacgc tgttttaaag attaatgcga tccatatcac gctgtgggta ttgcagtttt
      241 tggttttttg atcgcggtgt cagttctttt tatttccatt tctcttccat gggtttctca
      301 cagataactg tgtgcaacac agaattggtt aactaatcag attaaaggtt gaccagtatt
      361 tttatcttaa tgaggagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
      421 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
      481 gttggtgcga aagcgcgtta caagaaagcc gggcaattgc agtgccaggc agttttaacg
      541 atcagttcgc cgatgcagat attcgtaatt atgtgggcaa cgtctggtat cagcgcgaag
      601 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
      661 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
      721 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
      781 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
      841 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccggg atccatcgca
      901 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
      961 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatatca
     1021 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc accagcggga
     1081 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     1141 gcgtcacagc caaaagccag acagagtgtg atatctaccc gctgcgcgtc ggcatccggt
     1201 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
     1261 ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt cgataacgtg ctgatggtgc
     1321 acgatcacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     1381 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     1441 cagctgtcgg ctttaacctc tctttaagca ttggtttcga agcgggcaac aagccgaaag
     1501 aactgtacag cgaagaggct gtcaacgggg aaactcagca ggcgcactta caggcgatta
     1561 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     1621 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     1681 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     1741 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggttggtatg
     1801 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactg ctggcctggc
     1861 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccggcc
     1921 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     1981 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     2041 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caattgacct acatgggcct
     2101 cgggctttgc tacagcctgg tgaatattcc ttatggttca cttgctaccg cgatgaccca
     2161 acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg attgccgctt cattgacctt
     2221 tgtatgcctg gcatttctga taggaccgag cattaagaac tccagcccgg aagagatggt
     2281 gtcggtatac catttctgga cgattgtgct ggtgattgcc ggaatggtgc tttacttcat
     2341 ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg gcgcagccgt cattgaagat
     2401 cagtttgcaa accctgaaac ggaatcgccc gctgtttatg ttgtgcatcg gtgcgctgtg
     2461 tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg ttctacgcgc gctatgtgtt
     2521 aaatgatacc gggctgttca ctgtgctggt actggtgcaa aacctggttg gtactgtggc
     2581 atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt aaaaagaata ccttcctgat
     2641 tggcgctttg ctgggaacct gcggttatct gctattcttc tgggtttccg tctggtcact
     2701 gccggtggcg ttggttgcgt tggccatcgc ttcaattggt cagggcgtta ctatgactgt
     2761 gatgtgggca ctggaagctg ataccgtaga gtacggtgaa tacctgaccg gcgtgcgaat
     2821 tgaagggctc acctattcac tattctcatt tacccgtaaa tgcggtcagg caatcggagg
     2881 ttcaattcct gcctttattt tgggattaag cggatatatc gccaatcagg tgtaaacgcc
     2941 ggaagttatt atgggcatcc gcacatcaat tgccttagta ccttgcggat ttatgctact
     3001 ggcattcgtt attatctggt tttatccgct cacggataaa aaattcaaag aaatcgtggt
     3061 tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc agcgatatca ctaattaata
     3121 ttcaataaaa ataatcagaa catcaaaggt gcaactatga gaaaaatagt ggccatggcc
     3181 gttatttgcc tgacggctgc ctctggcctt acctctgctt acgcggcgca actggctgac
     3241 gatgaagcgg gactacgcat cagactgaaa aacgaattgc gcagggctga taagcccagt
     3301 gctggcgcgg gaagagatat ttacgcatgg gtacagggag gattgctcga tttcaatagt
     3361 ggttattatt ccaatattgt tggcgttgaa ggcggagcgt attatgttta taaattaggt
     3421 gctcgtgctg atatgagtac ccggtggtat cttgatggtg ataaaagttt tggctttgcc
     3481 ctgggggcag taaaaataaa acccagtgaa aatagcctgc ttaaattagg tcgcttcggg
     3541 acggattata gttatggtag cttaccttat cgtattccgt taatggttgg cagttcgcaa
     3601 cgtacattac cgacagtttc tgaaggagca ttaggttatt gggctttaac gccaaatatt
     3661 gatctgtggg gaatgtggcg ttcacgagta tttttatgga ctgattcaac aaccggtatt
     3721 cgtgatgaag gggtgtataa cagccagacg ggaaaatacg ataaacatcg cgcacgttct
     3781 tttttagccg ccagttggta tgatgatacc agtcgctatt ctctgggggc atcggtacag
     3841 aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa gcataccgct cgacccgaat
     3901 tatacgttga aaggggagtt gctcggcttt tacgcgcagc tcgaaggttt aagtcgtaat
     3961 accagccagc ccaatgaaac ggcgttggtt agtggacaat tgacctggaa tgcgccgtgg
     4021 ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa tgaatggtgc cgtggtggat
     4081 accgacattg gctatccctt ttcattaagt cttgatcgta accgtgaagg aatgcagtcc
     4141 tggcaattag gtgccaacta ccgtgtaact ccgcaattta cgctgacatt tgcaccgatt
     4201 gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga ttgaaggcgc gggtatctta
     4261 ggtggtatga actatcgggt cagcgaaggg ccgttacaag ggatgaattt ctttcttgct
     4321 gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg gcgatcgcct gaattactgg
     4381 gatgtgaaaa tgagtattca gtatgacttt atgctgaagt aagaaataac gccggagaga
     4441 aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt ttttatgccg gatgcggcgt
     4501 aaacgcctta tccagcctac aaaaactcat aaattcaaag tgttgcagga aaaggtaggc
     4561 ctgataagcg tagcgcatca ggcaatctct ggtttgtttt cagatgaaaa cgccggagtg
     4621 aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga attgccggta cagccggaac
     4681 gttaagagct ggcatcgcaa acatgccaac aaaatcttct aacgacattt tctgcccatt
     4741 taacgttatc tgaccgttag catattgcag gctggtggtg atggtattgt cctgcaaggt
     4801 ggtcagacgg aacatctgcc ccattgctga tgcaccttca acttgctgtt tcgccagttt
     4861 tttcgcttga tcttcctgat aaccttccag cttcgctacc tgagtcatca actcagttgc
     4921 catatccacc ggaatggtca gtttcgcatc cagagattta accgaacgat ctacttcctg
     4981 cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct ttcaggaaca gcgacagatt
     5041 cagcgcactt tcaccctgac tgtttttcca gcttagcggc gcgatagtaa tcaccggatc
     5101 gcctttcaac atcagcggca gggcgctaaa gaaggcttcc gtcactttct cctgataaag
     5161 ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc tgagtttgcg cgttatattg
     5221 ctggctaaac tgatgccagg cttcaccatc aatctggccg acttttaaag tcagcttgcc
     5281 gctgcccaga tcctgattct gtaccttcag gctgtttagc gagtaatcca gttggctatt
     5341 gatcgtttta ccgtcattga ccagatccga tttaccgctg atctccatgc cttccagcag
     5401 tgccagttct ttgccttcca ctgaaatggt cattttttcc agtgacagtt tttggtttcc
     5461 tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg gttttcagat tattaaaggt
     5521 caactgcact ttctggttgt attcgttaac tgcgtctatc cgaccacttt gcgcctctcc
     5581 ggaaagggag atggctttgc cgtctctgtc agcatttaac tggaactcgc cgccgctaaa
     5641 ggcgactttt tcatcctttt gctcgtaatt cagtggattg agcgaaatat cggaactgga
     5701 atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc gtttcacctt ttgccatatc
     5761 aaacagtggt ttgcttactt cgttattaac cagcgtggtt tgaattgatg ccatcgacgg
     5821 gatcaggttc agttttttaa gctgggcaag cgggaaggga ccatgatcaa ccgattcgtt
     5881 gaagatgacg ctctgaccgc ttttaatcca cggattttct ttcccggcaa tgggtttcac
     5941 caacagttgc aactggctgc tgaatacgcc gcgatgatag ttttgataac tcacttccag
     6001 gttggattca ggagctgtca gtttgagttg cgcgttcgcc tgcgcgacca tgtcttcgag
     6061 atgggtttca atcttcttgc ctgtatacca tgcgccgcct gtccagacta cgcctagcgc
     6121 aacaatgacg cctaccgcta ccagcgattt attcataatg attatccata aaatgaaatc
     6181 aggcggactg gccgcctgaa ggtgttataa gcctttaata agcttagcaa gagatgttaa
     6241 ttttttcagt aagctcttac agcttgttgt aaacacgcgc taaacggccg tggcctttga
     6301 cagtcaccgg tgattcgttg gcggcaataa acgctgattc acccggttta agctgtaact
     6361 gctgagaacc tttccacaac gttgcatcgc cttcgacgca gaacaaaatg gcggcactct
     6421 gctggctgat ggtggtttct ttatcactaa ggtcatgcag cgagaaggca aaatcatcca
     6481 caggaatcgg gaagtccagt tctgcacctt gtttcaccgg ctgggtcaac aactggttag
     6541 ccggtttggc ttcgaatttc acattggcaa ccagttccgg aatatcaatg tatttaggcg
     6601 tcagacctgc acgcagcacg ttatcggagt tcgccatcac ttccagcgcc acgccttgca
     6661 ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc gccaggattc aatttcacca
     6721 cattcagtaa cagcggggag aacaggccgc tgtcttccgg gtaaaattca gaaattaaac
     6781 gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc cgattttaaa atcgccagcg
     6841 cgcgggattt ttcttcaccc tgcatattca acaggttggc gaacagttcg cttaaacgtt
     6901 cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg tgcaccggcg accggctgga
     6961 gtagggagac aatctcggaa aattcacgaa acgcgttcat cgcaaggaaa ggcgtcagcg
     7021 caaaaaccag ctccggcttg tggttaggat ctttatagtt acgctcggcg gcatccatcg
     7081 ggatacctgc ggcattttct ttggcaaaac cgatttcaga attgtgtttg tttggatgaa
     7141 cctgaatgga gagtggctgt gctgcgcata atactttgaa caggaaaggc agttcgccaa
     7201 agcgtttggc aacggcctct ccgagcagag tcgatttatc actctcaatc acatcacgca
     7261 gtgaaacgat atctccggcg gcattctgca ctcgtgaact gcttttcgga tgtgcgccca
     7321 tccacagctc ggccatcggc tggctggacg gattttccat accataaagt tcagtcaacg
     7381 ccgttttgct accccaggca tagttttgca ctgagttaat gagtttttgc atgatcaatc
     7441 cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag aatcctacgg gaagtaacct
     7501 ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa aaactgtgta ggatattgtt
     7561 actcgctttt aacagggcaa cggaacaccc gcccagagca taaccaaacc aggcagtaag
     7621 tgagagaaca atgtcaaaca aaccctttca ttatcagtct ccttttccac tcaaaaaaaa
     7681 tgatactgag tattacctgc taaccagtga acacgttagc gtatctgaat ttgaagggca
     7741 ggagattttg aaagtcgcac ccgaagcgtt aactctgttg gcgcgtcagg cgtttcatga
     7801 tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg gccgacattc tgcgtgaccc
     7861 ggaggccagc gaaaatgata aatatgtggc gctgcaattc ctgcgtaact ccgacatcgc
     7921 ggcgaaaggc gttctgccaa cctgtcagga taccggcacc gcgattattg ttggtaaaaa
     7981 agggcagcgt gtatggaccg gtggtggtga tgaagcggcg ctggcgcgcg gtgtctataa
     8041 cacttatatc gaagataatc tgcgctactc gcaaaacgcg ccgctggata tgtataaaga
     8101 agtgaatacc ggcaccaatc tgccagcgca gatcgatctt tatgccgttg atggcgacga
     8161 atacaaattc ctctgtatcg caaaaggtgg tggttcggca aacaagacgt atctctatca
     8221 ggaaaccaaa gcgttactga cgccggggaa actgaaaaat tacctggttg agaagatgcg
     8281 cacgctgggt accgcggcct gtcctccgta tcatattgcg ttcgttattg gtggaacttc
     8341 tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg aaatactatg atgaactgcc
     8401 aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg gaactggaaa aagaattact
     8461 gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt ggtaaatact tcgctcacga
     8521 catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt ccggttggta tgggtgtttc
     8581 ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt caggggatct ggatcgaaaa
     8641 actggaacat aatccaggta aatatatccc tgaagagctg cgtaaagcgg gagaaggcga
     8701 agcggtgcgc gttgacctta accgtccgat gaaagagatc ctcgcacagt tgtcgcagta
     8761 tcccgtttct acacgcttat cgcttaacgg cacgattatc gttggtcgtg atattgctca
     8821 cgccaaactg aaagagcgga tggataacgg tgaagggctg ccgcaataca ttaaagatca
     8881 tccgatttac tacgcgggtc cggccaaaac gccggaaggt tatgcctccg gttctcttgg
     8941 cccaacgacc gccggacgga tggattctta tgtcgatcaa ctgcaagcgc agggcggaag
     9001 tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg acggatgcct gtaaaaaaca
     9061 cggcggcttc taccttggca gtatcggtgg tccggccgct gtattggcgc agggaagtat
     9121 taagagcctg gaatgtgttg aatatccgga actgggaatg gaagccatct ggaaaattga
     9181 agtggaagat ttcccggcgt ttatccttgt ggatgataaa ggaaatgact tcttccagca
     9241 gatacaactc acacagtgca ctcgctgtgt gaaataaaca gagccgccct tcggggcggt
     9301 ttttttacat ggcacgaaag accaaacatt tgttatcaaa tagtaaataa tgagtgagct
     9361 aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta atcaggtgag gagcaggcca
     9421 tgaatacagt acgcagcgaa aaagattcga tgggggcgat tgatgtcccg gcagataagc
     9481 tgtggggcgc acaaactcaa cgctcgctgg agcatttccg catttcgacg gagaaaatgc
     9541 ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc agcggcaaaa gttaatgaag
     9601 atttagactt gttgtctgaa gagaaagcga gcgccattcg gcaggcggcg gatgaagtac
     9661 tggcaggaca gcatgacgac gaattcccgc tggctatctg gcagaccggc tccggcacgc
     9721 aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc cagtgaatta ctcggcggtg
     9781 tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt gaacaaaagc caaagttcca
     9841 acgatgtctt tccgacggcg atgcacgttg cggcgctgct ggcactgcgc aagcaactca
     9901 ttccgcagct taaaaccctg acacagacgc tgagtgaaaa atcgcgtgca tttgccgata
     9961 tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc gctaacacta gggcaggaga
    10021 tttccggctg ggtagcgatg ctcgagcata atctcaaaca tatcgaatac agcctgcctc
    10081 acgtagcgga actggctctt ggcggtacag cggtgggtac tggactaaat acccatccgg
    10141 agtatgcgcg tcgcgtagca gatgaactgg cagtcattac ctgtgcaccg tttgttaccg
    10201 cgccgaacaa atttgaagcg cttgcgacct gtgatgccct ggttcaggcg cacggcgcgt
    10261 tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga tgtccgctgg ctggcctctg
    10321 gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa tgagccgggc agctcaatca
    10381 tgccggggaa agtgaaccca acccagtgtg aagcattaac catgctctgc tgtcaggtaa
    10441 tggggaacga cgtggcgatc aacatgggtg gcgcttccgg taactttgaa ctgaacgtct
    10501 tccgtccgat ggtgatccat aatttcctgc aatcggtgcg cttgctggca gatggcatgg
    10561 aaagttttaa caaacactgc gcagtgggta ttgaaccgaa tcgtgagcga atcaatcaat
    10621 tactcaatga atcgctgatg ctggtgactg cgcttaacac ccacattggt tatgacaaag
    10681 ccgcggagat cgccaaaaaa gcgcataaag aagggctgac cttaaaagct gcggcccttg
    10741 cgctggggta tcttagcgaa gccgagtttg acagctgggt acggccagaa cagatggtcg
    10801 gcagtatgaa agccgggcgt taatctgcaa catacaggtg cagccgtgga atgatcaaac
    10861 gaagcggctg cgcctgaggt ttataacggt gctgcacatt gtcggcatcg taatttaaca
    10921 actcaccaac gtccggcacg cccgcgccat tatcccgatt aatcagtgca atcagtggtg
    10981 tagggcaggc gtgttggact tgtttttgat ctcctttgta ccagacgcgg gcaatcggct
    11041 gcaccttcac tggacgtttg atttttaact tcgcgttctg tggcagggca gcgatatcct
    11101 gatactctcg ctccagtttt ctttgccact cctcgcgcgt ccacggtgcg acactgcgtg
    11161 gtgatttcag gcttttttcc agctgtgcca ggacttcatc acgatgcaaa ttcttaatga
    11221 tatgtttatt agcccaacca aagcgtaaag tggcggggtc gtgcagaacg gtgagcgtgc
    11281 ggtaagcatt aagggtgatc agccccggca aatgacgatg cacccattca aaacgtgccg
    11341 cggtggggag ttctgattca accgtgacga tatgctcgaa cgtggtcttg agtttattga
    11401 tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt atcgacctgg taacacaaca
    11461 cgccaggcag acggacagcg gccttgctgc tgcgattttc ggactgttgt tgaataaata
    11521 aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt gccgagatgt tgttttacct
    11581 caatacgatt aagcggatta tgctcatcct ctttttttac ctccggcaaa gagaacacgc
    11641 gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa tgtagccagc tcttgttcca
    11701 tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta acgcgccata atattaacca
    11761 ctttagttac aacatactta ttttatagca cagtcgtggt gacttgacca tcgtgcgcgt
    11821 agtgtcaggc agaggtaaat tgcgggatgt tatgccataa cggccagcta aacgagaagc
    11881 gagcaccacc cagttcgctg atgtcacaat taaccgtacc gcccattgtc agtgctattg
    11941 agtggacaat tgccagcccc agcccgcagc cgccggttga gcgatcccgg ctgggatcga
    12001 ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg ggcaatccct gggccatcat
    12061 cctcaacaat taatgtcgct ctattccccg acagtagcag gctggtttca accgttgaat
    12121 ggcagtagcg cagggcgtta ttgagcaaat tatccagcac gcgctccatt aagcgcatat
    12181 ccaacgccgc ataatggcct tgcatgagcg tttttatccg taccgtttta tcgggcgtta
    12241 ctgcctgaat atctgccaga tgcgttgaca gccacaacgg caggtctggt tcgctaagat
    12301 gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag cagctcttca attaaagctt
    12361 caagttgact gatatcacga ttcaacgcct gggattcggc ggcgctcagg ttatcgctca
    12421 tctccagtcg ataacgcagg cgcactaacg gtgttcgcag ttcgtgagcg ataccgtcaa
    12481 taagctgttt tttgctggca attaaggcgt tgatattgtc cgccatctgg ttaaatgcga
    12541 taccaagtcg ttcaaagctc gaaccctcat caaagtggat acgttcattg agatgcccat
    12601 cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc ctgccagtgc ggacgcatcc
    12661 agataaacac cggaaaggcg agggaaatag caataaaagc gatcagggcg atatccagca
    12721 atcgcatttg atggaggtaa taaagataag gaacaggacc aactgccagc acgtagtggc
    12781 tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag ggcgacaatt tcgccgccac
    12841 gcagtcggtg catggaaata tcatcaagat ggtatttact cagtggctcg acacgcagat
    12901 cgaaagagag atttaaatcc atctctttca gcgtcttacc ccagtcgtgt ggggggatct
    12961 ctcgcaattc gctgcgcatc agatacagcg aactgttcat caaatcatcc agcgactgtt
    13021 tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac cagcagagac atcacaagga
    13081 agcagacaaa caataacagg taaaactgga taaacaattt tttcattggc atcgcccgca
    13141 acagaatact attcgtatta attcacgact gtggagcaat gtaaaccgcc aactggataa
    13201 gaccttgttt ggaagataat gtccggaaca gacggatgta tccccggtgt attcatattc
    13261 agtaaattca attttccacc ggggcttgtt tattatgcta aggtttaatt taacgtgacg
    13321 ctttgttgtt ctttagtctc tctatcctta agaagacggg ctgatattac accggacatt
    13381 attgaaccat tcacattaag cgctgttctt gccatatcaa cgatcggctc tatggcaata
    13441 aaaataacta ctaatgcgac agggaaatta agtgttgaaa ggacaatcag cgccgcgaat
    13501 gtcccaccac tacctacgcc tgctacccct attgagccaa gcgcgatggc aggtaaaaga
    13561 gttgcgagga attgcaatga gaagggatcg atacccattg ttggagcaat catcgccacc
    13621 atcacggcag gataaatccc agcgcagcca ttttgtccca tgctggaacc aaaagacgct
    13681 gcaatattag caatcgtatt tggaaccccc aatttttcca atgcagaaat agccagagga
    13741 attgaagccg cactactgcg agaaacaaac gcaaaggtaa gtacttgcca gacggtcttg
    13801 taatagttaa cggggttttt acctattaac agcaaaatgc ccgagtgaac aataaacatc
    13861 acaaaaacgg ccagataaca ggcgacgatg aacccgagca aattggtaaa ctgtgaaagc
    13921 tgatacgttg agaagacttt cgccattagt gccattaccc cataaggcgt cagcgcaatg
    13981 acaatacgga ccattttcat tacccatatt tgaatcgcgt tgatccccgc gctcaatttt
    14041 tcgccctctg ctggtgcatc tttctttact tttaacagcg caataccagc aagaattgta
    14101 aagatgacga tgccaataac cgaaacactt cttgccccgg tcagatcaag gaagaggttg
    14161 gttgggatca atgacgtaat gagtcgcggt aaagataccg cagctgtttt cgagacgtca
    14221 tcgctggtta aagaggattg catatgacca aaggcgctgg catcaaggcc aattgcactg
    14281 gcaatagcca ggccaactaa acctgcaatc atgactaaac acaacataca ggcaacaata
    14341 gttaaagaca tcttgccaat accagcgcta ttttcgagct tattaatcgc tgaaagaatc
    14401 gaaatgaata tgagtggaac ggcaacaagt tttaataaat taacataacc gttaccagca
    14461 atattatacc attcgataac ggtttcactg cttgtctcag gaatgaaatg cagggagata
    14521 ccaatgatag cccccaggaa aatcgcgata aaaacacttt tggaaaggga ttcgctcttt
    14581 tgatccgttt tgaaaatccc ccatgagcag atcaaaaaaa gcacaatata tgtgaaagtt
    14641 gtaaccgttt tttaagcctc ttgcctcatt cagcacctga aatgccggaa agagtttgtt
    14701 gtaaaaaatc tgcgccataa gacgcaggga cgaggcgatt atagagaaat tttttctcat
    14761 tttcatatca aatgtagggg gtttggaatt tgaataagct cttattaatt gaattcgtta
    14821 attgttctct ttttcagatg aacaaatatt agtcatctga ttgtttattg tcactatccc
    14881 atgcatgagg cgcaaaaaga tagcctttgt tacgcacagt tttaatgcga taaggttctg
    14941 cggcgttatc gagcagtttt tttcttaacc gcgaaatagc cacgtccacg ctacgatcca
    15001 gtccgtcata actgacgccg cgtaaatttt tcagcaatgc atcgcggtcc atgatttgcc
    15061 cggcatgcgt agctagttcc cacaataatt cgaaatcagc tgtcgagagc gagatttcag
    15121 tgttagccag ggtgactacg cggttgatgg ggtcgatagt taacgtgccg aaatgcaggg
    15181 ctttgtaggg agtaagagac gtttcctgaa gacctttggt cagtgtggct tgctcattct
    15241 gacgcaaatg caaacgtaaa cgcgctagca aaacagcagg gggcgtcgtt ttgagaatat
    15301 agtcgcaggc acccatttcc agtgccagga tgtggttcat atcgctatcg agagaggtta
    15361 gaagaacaat cggtccagac cactttgcgc gtaaatcacg acaaatggtc atgccgtcct
    15421 tgcctggtag catgatgtcg agtaacacca aatccggatt ttctcgcaaa atggtttctt
    15481 cggcctggtc gccgcgcggt tctacggtaa cctgcatatc atgttttgcc aggtacgcgg
    15541 caatcagtga accgacttcc gcatcatctt ccacaaatac gatagtgttc ataacattca
    15601 cggtagatat aaaaacgtca caatacaccg cgctgttttt acttactatc aatcgtttct
    15661 aaacaagttt tcatcaggta ttctactgat gacttgttat ggaagtgtta aggtaaaaag
    15721 atggggctgg taatcaaagc cgcgctcggt gcgctggtag tgctgttgat tggtgtttta
    15781 gcaaaaacga aaaattatta tatcgccggg ctgattccac ttttcccgac ctttgcgctt
    15841 atcgcgcatt atattgttgc cagcgaacgt ggcattgaag ccttacgcgc aaccatcatt
    15901 tttagtatgt ggtcgattat tccctatttt gtttacctgg tgtcgctgtg gtatttcacc
    15961 ggaatgatgc gcttgcccgc cgcttttgtt ggttctgttg cgtgttgggg gataagcgca
    16021 tgggtgttga ttatatgctg gataaagctg cattaacgca gatgacgacc gccatcaagt
    16081 gggaaactgc gtccggtgac aaagcaactg gtaagtaagt aatcgaccag gtcgatcact
    16141 tctttctcgc caggcgcggt tttcatcagt gatttattca gcgcctgttg tcgatattcg
    16201 gcatcatcat gttcattaaa caggatcagc gatggcgcaa tagaattcac tttcacttcc
    16261 ggtgccagct tgcgggcaaa cgagcgggtc atattatcca gtgccgcttt gcttgcagca
    16321 tacgcaacat gtttgtcgct accgcgctcc accacataat cggtaaagtg aatgatatcg
    16381 ctggcggcgt gtccgtgccc acgcagtaat ctttccagcg catggttgag caggtatggg
    16441 gtattaacgt ggatctgcat catgcaagcc agtacgtcgg ccagtggcgc acccggtttt
    16501 tccgcaatcc acgcactggc gttatgcaaa atagcacgca gaccatgggt ggtttttagt
    16561 acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc
    16621 gcacctgcat taatcagtcc atcaatggct ggatagtgtg tccgatagct gacaatcacc
    16681 ggttgctttt gattaatgaa atgccatgcg agggcgaggc cgatgcgacg acctccgcca
    16741 gtaattaata ttggcaaggg ctgggtttta cccatcgtta tctcctttgc tatccaacga
    16801 tgggatgaac gttatcccac cagcatccag gtcgccggaa ctgaggcaat taacagcatg
    16861 ccaatcagca ccatttcctg acggttcagt acattatcat gtgtatgcgt tttgcgcgca
    16921 tacaggaaaa ccagcagccc cggtgcatac agaacaacgg ataacagcaa gtgcattgga
    16981 ccagaggcgt acaataacca taagccataa atgcaggcac cgacacctac cgctttatgc
    17041 agtggacggg ttgcgatttt cagcaagaac gcgccgacca ggaaataggg caccagaatc
    17101 atttctgagg cgatggtcag caacgtgttg taatcggaac cggtcagcca gatgagtacc
    17161 agacaaattt gcacgcagat gttggtcaac cacagtgagg cggatggcgc agcttgtgcg
    17221 ttctggcgcg caaaaatgcg tgggaatgct ttatgagtgg cagccaggaa cggaacttct
    17281 gccgccatga tggtccagct taagtacgcg ccgcaaacgg aaacgatcaa accggcagcg
    17341 atgatgattt cgccccatgg tcccatcatt tccaccatca gaccggccat tgacgggtta
    17401 cgaatttcag ccagttcagg acgtgccacc acacccagcg aaagcagcgt taccagcaag
    17461 taaacgccca gagcggagag aaccgccagc agtgtcgctt tgccaacatc acgtttatta
    17521 cgcgcacgcg cagaaacaac cacggcacct tccacaccaa tgaaaaccca cagggtgatc
    17581 agcatggtgt ttttcacctg ttcccaaaca ggtacgccaa gtgcaagtcc ggtgaagtcg
    17641 agcttgaagg tatccagttt gaacatcatc atcgccagca caacaaacag acccagcggc
    17701 aacaattttg ccagtgtcgc caccaggtta atgctggcag cggtttgcac cccgcgcagg
    17761 atcaaaaagt gaacaatcca caataaagcc gatgcaccga ctatcgattg ccaggtatta
    17821 ccatcgccaa acaggcgcaa ttccggcgtg tccgtaaaaa agcttaacgc ggaaaaaacg
    17881 atcaccagat aggagacgtt ggcgatgacc gcgcacagcc agtatcccca tgcggaacaa
    17941 aagccgatta gctcgccaaa cccttcgcgg gcataggtaa agataccgcc atcaagttcg
    18001 ggacgaatgc gcgtgaggat cagcatggca aaggccagca ataaaatgcc agcgccagta
    18061 ataccccagc cgatgagcag tgctgccggg ctggcaactg ccgccatatt ttgcggcaga
    18121 ctgaaaacac ccgcgcccag cattgagctt aataccagcg cggtgagtgc gctcagtccc
    18181 agtttctttt ccatcgatat ccagtgatta aaacgtgata agaagaataa atattagggc
    18241 aaaacgcata aaaatggagt ttgccactga agcgcgggat tttacgaaga gatgtgtctg
    18301 catgcaatgg tcgcggggaa atttgtgact aaaaatgcga aaggcggcat caagccgcct
    18361 tataggagta actgaatagt tatttataca gatctgcgct gacggtcagg ttattaccac
    18421 gtttctgcca ctggcgggtg atgtggtaat acttagcacc tttcttcgcg gcacgtttcg
    18481 caacctgata ggagacttcg gtcatgttgc cgtagttgcc agagaatttg atgctgtcga
    18541 acgggaccat catcgctgct gtcgctttgt tcagttcttc gactttagtg ccatccggga
    18601 gcgtgacggt gtaacgcccg ccttttgatg actgggtttc aaagaagcga ccgacttcag
    18661 aacttggtga tgcggtagtc gcaacacccg ggatctcaac tttcttcgcg gcttcgccac
    18721 cggcagccag agctgcacgt cctgcttcgg aatctgccgg gatcacatcc gggctctgga
    18781 cgatacgttt cttagcatct tttttataga tgaatgcagt aatacgctgg ttgccgccct
    18841 ggttggcatc gatttgacga acgatgtaga aagagtaggc acctttcgct tttgccgctt
    18901 tggtgatggc atcattgact tctggctggc tacgatagaa gccctggacg gtgaccgtgt
    18961 caaacggttc aatcagaaca gcctgatctt tcggcagttc gacaacaccg ttaattacgc
    19021 gattacttgt ttcttctgct ttttcagcat cggctttata gaggtcagcg accacacgcc
    19081 agttaccgct gttaccaaaa tcagaagtgt cgacaacata aaaagaggcg gcaccttctt
    19141 tatctgcgcg acgagaaacg gctttcaccg cttcgccaat agcattaaaa cgaccggtaa
    19201 ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc cggtgtcagt tctgttgctg
    19261 cgttaacgga gaatgccgta gcagaaagca gtgccgacgc caggagggtg ttcttaagct
    19321 tcataaaaat aatccttcgc cttgcgcaaa ccaggtactg gtattgttat taacgagaaa
    19381 cgtggctgat tattgcattt aaacggtgta actgtctgcg tcatttttca tatcacattc
    19441 cttaaaccaa ttttaatcct gctcaaatga ccgtctatgc ttaaaaaaca gccgtatcag
    19501 catcattact actgaagcaa ctgaattgta taagttaatt taatgttaag tagtgattcg
    19561 tgccggggcg atgtctcgtt ttacccgacc gtcgaagaca attatcagtc tttatccggc
    19621 gttctaaggt gtttatccca ctatcacggc tgaatcgtta atattttgcg agttcacgcc
    19681 gaaatactga tttttggcgc tagatcacag gcataatttt cagtacgtta tagggcgttt
    19741 gttactaatt tattttaacg gagtaacatt tagctcgtac atgagcagct tgtgtggctc
    19801 ctgacacagg caaaccatca tcaataaaac cgatggaagg gaatatcatg cgaattggca
    19861 taccaagaga acggttaacc aatgaaaccc gtgttgcagc aacgccaaaa acagtggaac
    19921 agctgctgaa actgggtttt accgtcgcgg tagagagcgg cgcgggtcaa ctggcaagtt
    19981 ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt agaagggaat agcgtctggc
    20041 agtcagagat cattctgaag gtcaatgcgc cgttagatga tgaaattgcg ttactgaatc
    20101 ccgggacaac gctggtgagt tttatctggc ctgcgcagaa tccggaatta atgcaaaaac
    20161 ttgcggaacg taacgtgacc gtgatggcga tggactctgt gccgcgtatc tcacgcgcac
    20221 aatcgctgga cgcactaagc tcgatggcga atatcgccgg ttatcgcgcc attgttgaag
    20281 cggcacatga atttgggcgc ttctttaccg ggcaaatcac tgcggccggg aaagtgccac
    20341 cggcaaaagt gatggtgatt ggtgccggtg ttgcaggttt ggccgccatt ggcgcagcaa
    20401 acagtctcgg cgcgattgtg cgtgcattcg acacccgccc ggaagtgaaa gaacaagttc
    20461 aaagtatggg cgcggaattc ctcgagctgg attttaaaga ggaagcgggc agcggcgatg
    20521 gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga aatggaactc tttgccgccc
    20581 aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat tccaggcaaa ccagcgccga
    20641 agctaattac ccgtgaaatg gttgactcca tgaaggcggg cagtgtgatt gtcgacctgg
    20701 cagcccaaaa cggtggcaac tgtgaataca tcgtgccggg tgaaatcttc agtacggaaa
    20761 atggtgtcaa agtgattggt tataccgatc ttccgggccg tctgccgacg caatcctcac
    20821 agctttacgg tactaacctc gttaatctgc tgaaactgtt gtgcaaagag aaagacggca
    20881 atatcactgt tgattttgat gatgtggtga ttcgcggcgt gaccgtgatc cgtgcgggcg
    20941 aaattacctg gccggcaccg ccgattcagg tatcagctca gccgcaggcg gcacaaaaag
    21001 cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc accgtggcgt aaatacgcgt
    21061 tgatggcgct ggcaatcatc ctgttcggtt ggctgtcaag cgttgcgccg aaagagtttc
    21121 ttgggcactt caccgttttc gcgctggcct gcgttgtcgg ttattacgtg gtgtggaatg
    21181 tatcgcacgc gctgcataca ccgttgatgt cggttaccaa cgcgatttca gggattattg
    21241 ttgtcggagc actgttgcag attggccagg gaggctgggt tagcttcctt agttttatcg
    21301 cggtgcttat agccagcatt aatattttcg gtggcttcac cgtgactcag cgcatgctga
    21361 aaatgttccg caaaaattaa ggggtaacat atgtctggag gattagttac agctgcatac
    21421 attgttgccg cgatcctgtt tatcttcagt ctggccggtc tttcgaaaca tgaaacgtct
    21481 cgccagggta acaacttcgg tatcgccggg atggcgattg cgttaatcgc aaccattttt
    21541 ggaccggata cgggtaatgt tggctggatc ttgctggcga tggtcattgg tggggcaatt
    21601 ggtatccggc tggcgaagaa agttgaaatg accgaaatgc cagaactggt ggcgatcctg
    21661 cacagcttcg tgggtctggc ggcagtgctg gttggcttta acagttatct gcatcatgac
    21721 gcgggaatgg caccgattct ggtcaatatt cacctgacgg aagtgttcct cggtatcttc
    21781 atcggggcgg taacgttcac gggctcggtg gtggcgttcg gcaaactgtg tggcaagatt
    21841 tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga acctggcggc tctggtcgtt
    21901 tccttcttgc tgctgattgt atttgttcgc acggacagcg tcggcctgca agtgctggca
    21961 ttgctgataa tgaccgccat tgcgctggta ttcggctggc atttagtcgc ctccatcggt
    22021 ggtgcagata tgccggtggt ggtgtcgatg ctgaactcgt actccggctg ggcggctgcg
    22081 gctgcgggct ttatgctcag caacgacctg ctgattgtga ccggtgcgct ggtcggttct
    22141 tcgggggcta tcctttctta cattatgtgt aaggcgatga accgttcctt tatcagcgtt
    22201 attgcgggtg gtttcggcac cgacggctct tctactggcg atgatcagga agtgggtgag
    22261 caccgcgaaa tcaccgcaga agagacagcg gaactgctga aaaactccca ttcagtgatc
    22321 attactccgg ggtacggcat ggcagtcgcg caggcgcaat atcctgtcgc tgaaattacc
    22381 gagaaactcc gcgctcgtgg tattaatgtg cgtttcggta tccacccggt tgcggggcgt
    22441 ttgcctggac atatgaacgt attgctggct gaagcaaaag taccgtatga catcgtgctg
    22501 gaaatggacg agatcaacga tgactttgct gataccgata ccgtactggt gattggtgct
    22561 aacgatacgg ttaacccggc ggcgcaggat gatccgaaga gtccgattgc tggtatgcct
    22621 gtgctggaag tgtggaaagc gcagaacgtg attgtcttta aacgttcgat gaacactggc
    22681 tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca cccacatgct gtttggtgac
    22741 gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac cctgacggcc tctgcggagg
    22801 ccgtcactct ttattgagat cgcttaacag aacggcgatg ctttgacctc ccgctgtttg
    22861 ttcaagcgca attttgacaa taattgtcaa cggcacggaa agcagcatac ccaccggtcc
    22921 taacaaccat ccccaaaaaa tcaacgacaa aaataccacc aatgtcgaaa gccccagccc
    22981 acgtcccatg atgcgcggct ccagaatatt gccgaagacc agattaatca gcagatatcc
    23041 cgccagcacc agcaacgctt cgtagaagcc attaaacacc agtacctgag cgataggggg
    23101 gattgccgcg agaactgaac caatattagg gatgtaatta agcgcaaagg ccagcaatcc
    23161 ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc gcccaggcga ccaggccggt
    23221 gatgatgctg atggctgttt tcagcaccag ataatgagaa acactgtcaa tcgcacgttg
    23281 aatcgccgcc atcccttcaa ccggacgcgc catcatttgc tgaaattttc cgggcaattg
    23341 tggcacttcg agcagcataa acagcaccgt cagcagcaat aaaaatattg atgacatggc
    23401 attagataac tgcgtcaata agttggtgag caacgtcatc gccgcgttcg gatcaatgta
    23461 atgcgccagt tggtcaactg agacgtcaat ccctacgcgt tgcaacaacg gctcaagagc
    23521 ttgcagcggc gtcataatag agttgcgata ttgcggtaac gtccgcgtca actcgttgag
    23581 cgcggaaccc agataagcta atagcaacac catcgccatc acgatgattg tcatcaaaat
    23641 cgacactgcc agtacacgcg gcacacgcca gcggaccata tgttgcacca gcgggttaag
    23701 aataacagca ataaataatg cgagaataaa cggcacgatg atctcggcgg caaaacggat
    23761 accgcagaga ataatgacca gcattcccaa cataatgacg atttttaggc cattgagcgt
    23821 gatgatcggc tttgccatgc ttgtccccgt ttttcttcct ttgctaataa taatgtcttt
    23881 ttcgctccgt catcataacg aaaatcaaac cgctcaggta aagaagtgaa aatattttga
    23941 gttaattctt aagctatgat acaaatcagg cgtgttcaac taccgaggac aattatcatc
    24001 tgcgatgacg agaagcaaca ctgcggataa ttgtaatatt atggacaatt tgttcaggac
    24061 gttattttct acatttaccc atcttcgtac ctcatccact atcttgctcg taggtgagca
    24121 gcactggcgc aacgcgcttt agtcctttct tctgcctaag cttgccctat gcgcagggct
    24181 tgaccacttt actttggttt cgctgaatta agcgaaaatt aaaatagttc tcttgcagga
    24241 gaaggacaat gtatatttat tggattttat taggtctggc tattgctaca gaaattaccg
    24301 gtacgctgtc aatgaaatgg gcgagcgtca gtgagggaaa tggcggcttt attttaatgc
    24361 tggtgatgat ttctctgtcg tatatatttc tctctttcgc cgttaaaaaa atcgccttag
    24421 gcgtagctta tgcgctgtgg gaaggtatcg gtattttatt tattaccttg tttagcgttt
    24481 tgttattcga cgaaagttta tcgctgatga aaattgccgg gttaaccacc ctggtcgccg
    24541 ggatagtgtt gatcaaatca ggtacccgta aagcgcgtaa acctgaactg gaggtgaacc
    24601 atggcgcagt ttgaatgggt tcacgccgcc tggctggcat tggcaatcgt gctggaaatc
    24661 gttgctaacg tctttttgaa attttctgac ggctttcgtc gcaaaatatt tggcttgctc
    24721 tccctggcgg cggtgctggc ggcctttagt gcgctttctc aggccgttaa agggatcgac
    24781 ttgtctgtcg tttatgcatt gtggggcggg tttggtattg ccgccacgtt agccgcaggt
    24841 tggatcttgt ttggtcaacg gttaaatcgt aaaggctgga ttggcctggt cttgctgttg
    24901 gctggaatga tcatggtgaa acttgcctga tgaagacgct gcccgcgctg tcgggcagcg
    24961 tttgaacatt atttttgcga cagttgatcc agcttgtcgc gaaaaccggt aacagaaatg
    25021 gcccggttat cggcgcgcca gcgatctttc gcggcaggag ccgaactttg caccccaatt
    25081 aattgccagc cgtcatcggt atgcaacatc agaggcgaac cgctgtcacc cggcaaggta
    25141 tcgcactgat gtgacatcac cgacgtttgc gcccagccag tcacttcaca gttttgatga
    25201 ctgtataacg tatcgagatg atcttcaggg tatcccgcct gagtcacttt acgaccagcc
    25261 gattttaatg cggcagtaag cgcggcttta tctccctcaa ataacggcaa cggtgtaatg
    25321 ccagaagggg gattacgtag cacaatcaat ccgaagtccc acggtgcagc tgcgggagga
    25381 acaatccaac catccccatc tgcttttaac cgctttccca gtgtcggatc aacgcggcct
    25441 tctatgtcgt ggatctcata gcgccaaaga cctttatttg acacaaaacg cagcgctacc
    25501 gccttatccg ctttaccctt aggcggcgtc aataaacagt gtcctgccgt taatgccaga
    25561 ttgggtgcaa tcagcgtcgc cgtacataaa ttgccgctgg ccgtttccag ttgcccaacc
    25621 gcatcccacg gtgattgggt cgtgtcattc actggcacac gatcatcatg accaaaaaac
    25681 agggtgctga cctcatcgtt tgccgatttg gcaacgtctg gtttatctgc aaacacaaaa
    25741 gcagacgtca aactaattgc acccaacact acagcaatgg ttgtacgcat atcacactct
    25801 ggtgggtaat tatgattatt aaaagcaaac cctcataaat actatagacg ggacgggatg
    25861 aaagtgggag taaaatcaga taactacagt caggaaagat aaaaacgggc ggcaatcagt
    25921 gcgcataaaa taaggatgat aagaatgaac tcaaagcgat aacggcccac catactaccc
    25981 tccgctaaag gcggtgctaa acgttacccg cttagcaccg taattttcag gcgcgagggg
    26041 gcgcgccagc attactgttg aaaacttacg ctgcgggttg tgcagcaggt tttgccggtt
    26101 gctgatggcc gtgtttcccg gcgtgtttct tcgctgcctg cgctttttgt tcaggggctt
    26161 tctgttcagc tttcgtattt ttatgatgct ttttagccgc ctgcgctttc tgggcaggtg
    26221 ctgctttatg ctgtttttta tgatgtgtag ttttcgctgg cgctgctttg gtggtcgtcg
    26281 cagtcggagc aggtgtggtc gcagtctctg cagcaaaggc ggcagaagac agacccatag
    26341 cagcggcaac aaccagagct aatacttttt tcattgtcat accctcaatt tgttttttca
    26401 tttaacccca ctgcggggcc gttgaaataa ctatatccct gggaatggca gacttccgtg
    26461 agtggttggt ttcagcgtgt aacgatatgt acaaacgctg aataaattac agcgttgata
    26521 ataacgtatt gtgacttaag gaaaatttag ctaccaataa tagtagtctt gatcgggtta
    26581 actattatac tacttacaaa gcggtagaat aactgcgcat cagtaataaa tgacacacag
    26641 caaaatgaat ccgtttattt gggtacttat aatcctgatg acgctagatg cgctgcggga
    26701 attggctggc gcttcgtcta ttttaggatg gctattaacg ttggtttgag caggcaataa
    26761 gtccggacgg gtatttaccg cagtccggac ttatttttca ggcgtgcaga cgacgatgca
    26821 aacgcgtccc gaccagcaga gcaatgacca gcatcagagc aataaatgcg ccgacgccgt
    26881 tccagccata gttatgccag aaaacaccac ccagcgtccc ggcaatactc gaccccagat
    26941 agtaactgaa cagatacagc gaggaggcct ggcctttagc gcgttttgcg cgggggccga
    27001 tccagctgct ggctactgag tgggctgcga agaatccggc tgagaagagt aacattccgg
    27061 caaagatcag ccacagcgag ctaaataagg tcatcagtaa accaaacggc ataactcccg
    27121 tcgaaaacaa catcaccgga ccgcgcccat agcgggtggt catggttccg gctttgggtg
    27181 agctccatgt accggtcaaa taagccagcg ataataagcc aaccacggcc tgactgacat
    27241 gctaggggga aagcatcaac cgatagccga tgtaattaaa cagcgtgacg aacgacccca
    27301 tcagcaaaaa gccttctgcg aacaataacg gtaatccccg gtcacgccag tgcagacgaa
    27361 agttgataaa taacgtctta gggcgcagcg aagtcgggca aaaatggcgt gattcaggga
    27421 ggattttcca gaacatcaac gccgaggcca gcgcgaaaca accgattgcc gccagagcaa
    27481 ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc atgccgccaa
    27541 ttgagttgcc gctgatatac aaccccatcg aaaaggccac gaaactgggg tggatttcct
    27601 cgctaagata agtcatgcca acagctgcca cgccacttaa cgaaagccca atcaaggcgc
    27661 gcataatcaa aatgccgtgc cagctggtca tcattgtcga aagtaacgta caaatggagg
    27721 ccaacagtag cgccgtgacc atcactggtt tgcgaccaat ggcatcggat agcgggccag
    27781 taaatagcaa accgatagcc aacatcgccg tggaaatgga cagtgaaata ctactgttcg
    27841 cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat aggctgcaca caatagagaa
    27901 gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt gacgcgcata aattgcggcg
    27961 taccgcgttt aataaattga tttggctgag aaatgctttg cttgtcagtg tcgcttgccg
    28021 gagcgccatc aacagttgta gtacggctca cttgaaatcc ttgctaaata tgcctgtgga
    28081 tcaggcttat acatagggta ggaaaatcga attgttctgt ctaatatatt aataatctca
    28141 aataagatgt tttaaatatg aatattgaac ttcgtcatct gcgttacttt gttgctgttg
    28201 cggaagagct gcattttggg cgcgcggctg cccgcctgaa tatttcgcaa ccgccgctaa
    28261 gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg actgctggca cgaactaatc
    28321 gcagtgtatt gctgacggca gcaggaaaac agtttcttgc agatagtcgg caaatcctgt
    28381 ctatggtgga tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa gcgggggagt
    28441 tgcgtattgg ttttacttcg tccgctcctt ttattcgggc ggtgtccgat acgttatcgc
    28501 tgtttcgccg tgattatcct gatgtccatt tacaaacccg cgaaatgaac actcgcgagc
    28561 aaatcgctcc gctcattgaa ggaacgctgg atatgggatt gctgcgtaac acagcgttac
    28621 cggagacgct tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg atcccgtacg
    28681 atcatcccct ggcaaataat ccgagtgtaa cactggctga actggctgaa ctggctgaac
    28741 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    28801 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    28861 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    28921 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    28981 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    29041 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    29101 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    29161 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    29221 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    29281 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    29341 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    29401 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    29461 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    29521 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    29581 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    29641 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    29701 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    29761 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    29821 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    29881 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    29941 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30001 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    30061 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    30121 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    30181 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    30241 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    30301 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    30361 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    30421 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    30481 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    30541 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    30601 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    30661 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    30721 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    30781 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    30841 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    30901 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    30961 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31021 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    31081 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    31141 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    31201 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtctgc
    31261 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    31321 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    31381 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    31441 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    31501 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    31561 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    31621 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    31681 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    31741 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    31801 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    31861 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    31921 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    31981 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    32041 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    32101 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    32161 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    32221 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    32281 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    32341 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    32401 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    32461 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    32521 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    32581 taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg
    32641 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    32701 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    32761 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    32821 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    32881 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    32941 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33001 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    33061 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    33121 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    33181 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    33241 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    33301 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    33361 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    33421 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    33481 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    33541 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    33601 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    33661 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    33721 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    33781 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    33841 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    33901 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    33961 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34021 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    34081 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    34141 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    34201 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    34261 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    34321 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    34381 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    34441 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    34501 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    34561 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    34621 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    34681 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    34741 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    34801 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    34861 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    34921 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    34981 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    35041 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    35101 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    35161 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    35221 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    35281 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    35341 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    35401 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    35461 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    35521 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    35581 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    35641 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    35701 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    35761 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    35821 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    35881 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    35941 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36001 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    36061 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    36121 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    36181 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    36241 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    36301 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    36361 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    36421 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    36481 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    36541 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    36601 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    36661 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    36721 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    36781 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    36841 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    36901 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    36961 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37021 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    37081 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    37141 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    37201 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    37261 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    37321 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    37381 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    37441 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    37501 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    37561 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    37621 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    37681 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    37741 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    37801 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    37861 tcatgcgttt tgttgatgtc agaat
//
LOCUS       DAJVPT010000028        37178 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVPT010000028 DAJVPT010000000
VERSION     DAJVPT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..704)
                     /gene="yhdJ"
                     /locus_tag="OQM47_001389"
     CDS             complement(<1..704)
                     /gene="yhdJ"
                     /locus_tag="OQM47_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1479604.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIA"
     gene            complement(790..1086)
                     /gene="fis"
                     /locus_tag="OQM47_001390"
     CDS             complement(790..1086)
                     /gene="fis"
                     /locus_tag="OQM47_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1479605.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1112..2077)
                     /gene="dusB"
                     /locus_tag="OQM47_001391"
     CDS             complement(1112..2077)
                     /gene="dusB"
                     /locus_tag="OQM47_001391"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1479606.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2406..3287)
                     /gene="prmA"
                     /locus_tag="OQM47_001392"
     CDS             complement(2406..3287)
                     /gene="prmA"
                     /locus_tag="OQM47_001392"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1479607.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3299..4750)
                     /gene="panF"
                     /locus_tag="OQM47_001393"
     CDS             complement(3299..4750)
                     /gene="panF"
                     /locus_tag="OQM47_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1479608.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4740..4982)
                     /locus_tag="OQM47_001394"
     CDS             complement(4740..4982)
                     /locus_tag="OQM47_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1479609.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5091..6440)
                     /gene="accC"
                     /locus_tag="OQM47_001395"
     CDS             complement(5091..6440)
                     /gene="accC"
                     /locus_tag="OQM47_001395"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1479610.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6451..6921)
                     /gene="accB"
                     /locus_tag="OQM47_001396"
                     /gene_synonym="fabE"
     CDS             complement(6451..6921)
                     /gene="accB"
                     /locus_tag="OQM47_001396"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1479611.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(7899..8873)
                     /locus_tag="OQM47_001397"
     CDS             complement(7899..8873)
                     /locus_tag="OQM47_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1479612.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9025..10965
                     /gene="csrD"
                     /locus_tag="OQM47_001398"
     CDS             9025..10965
                     /gene="csrD"
                     /locus_tag="OQM47_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1479613.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11270..12313
                     /gene="mreB"
                     /locus_tag="OQM47_001399"
     CDS             11270..12313
                     /gene="mreB"
                     /locus_tag="OQM47_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1479614.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12379..13482
                     /gene="mreC"
                     /locus_tag="OQM47_001400"
     CDS             12379..13482
                     /gene="mreC"
                     /locus_tag="OQM47_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1479615.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13482..13970
                     /gene="mreD"
                     /locus_tag="OQM47_001401"
     CDS             13482..13970
                     /gene="mreD"
                     /locus_tag="OQM47_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1479616.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            13979..14572
                     /gene="yhdE"
                     /locus_tag="OQM47_001402"
     CDS             13979..14572
                     /gene="yhdE"
                     /locus_tag="OQM47_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1479617.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14562..16031
                     /gene="rng"
                     /locus_tag="OQM47_001403"
     CDS             14562..16031
                     /gene="rng"
                     /locus_tag="OQM47_001403"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1479618.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16099..19899
                     /gene="yhdP"
                     /locus_tag="OQM47_001404"
     CDS             16099..19899
                     /gene="yhdP"
                     /locus_tag="OQM47_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1479619.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20055..21500
                     /gene="tldD"
                     /locus_tag="OQM47_001405"
     CDS             20055..21500
                     /gene="tldD"
                     /locus_tag="OQM47_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1479620.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21628..22557)
                     /gene="aaeR"
                     /locus_tag="OQM47_001406"
     CDS             complement(21628..22557)
                     /gene="aaeR"
                     /locus_tag="OQM47_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1479621.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22740..22943
                     /locus_tag="OQM47_001407"
     CDS             22740..22943
                     /locus_tag="OQM47_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1479622.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            22951..23883
                     /gene="aaeA"
                     /locus_tag="OQM47_001408"
     CDS             22951..23883
                     /gene="aaeA"
                     /locus_tag="OQM47_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1479623.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            23889..25856
                     /gene="aaeB"
                     /locus_tag="OQM47_001409"
     CDS             23889..25856
                     /gene="aaeB"
                     /locus_tag="OQM47_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1479624.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            25948..26220
                     /locus_tag="OQM47_001410"
     CDS             25948..26220
                     /locus_tag="OQM47_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1479625.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26276..26539)
                     /gene="yhcN"
                     /locus_tag="OQM47_001411"
     CDS             complement(26276..26539)
                     /gene="yhcN"
                     /locus_tag="OQM47_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1479626.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(26904..27374)
                     /gene="argR"
                     /locus_tag="OQM47_001412"
     CDS             complement(26904..27374)
                     /gene="argR"
                     /locus_tag="OQM47_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1479627.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27809..28747
                     /gene="mdh"
                     /locus_tag="OQM47_001413"
     CDS             27809..28747
                     /gene="mdh"
                     /locus_tag="OQM47_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1479628.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28809..29876)
                     /gene="degS"
                     /locus_tag="OQM47_001414"
     CDS             complement(28809..29876)
                     /gene="degS"
                     /locus_tag="OQM47_001414"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1479629.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(29966..31313)
                     /gene="degQ"
                     /locus_tag="OQM47_001415"
                     /pseudo
     CDS             complement(29966..31313)
                     /gene="degQ"
                     /locus_tag="OQM47_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31467..31865)
                     /locus_tag="OQM47_001416"
     CDS             complement(31467..31865)
                     /locus_tag="OQM47_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1479630.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32059..33186
                     /gene="zapE"
                     /locus_tag="OQM47_001417"
     CDS             32059..33186
                     /gene="zapE"
                     /locus_tag="OQM47_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1479631.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33405..33833
                     /gene="rplM"
                     /locus_tag="OQM47_001418"
     CDS             33405..33833
                     /gene="rplM"
                     /locus_tag="OQM47_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1479632.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33849..34241
                     /gene="rpsI"
                     /locus_tag="OQM47_001419"
     CDS             33849..34241
                     /gene="rpsI"
                     /locus_tag="OQM47_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1479633.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34636..35274
                     /gene="sspA"
                     /locus_tag="OQM47_001420"
     CDS             34636..35274
                     /gene="sspA"
                     /locus_tag="OQM47_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1479634.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35280..35777
                     /gene="sspB"
                     /locus_tag="OQM47_001421"
     CDS             35280..35777
                     /gene="sspB"
                     /locus_tag="OQM47_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1479635.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35820..37178)
                     /gene="dcuC"
                     /locus_tag="OQM47_001422"
     CDS             complement(35820..37178)
                     /gene="dcuC"
                     /locus_tag="OQM47_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1479636.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVI
                     ATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDK
                     KDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLL
                     SLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVD
                     AVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVPAVV
                     MIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITL
                     FY"
BASE COUNT         8994 a         9308 c         9813 g         9063 t
ORIGIN      
        1 ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg
       61 cctgggttgg aagaggcgag aataatgcgt ttcagtaagg ctttcggttt ttgcgtcggg
      121 tggttctcat attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt
      181 actttttgat gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc
      241 gatccggttt tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct
      301 ttcaccatca tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca
      361 gaactgtcat atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga
      421 tcgataaagg gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga
      481 acgcggtggc actctgcaat cacttcaaac agccagtcga taaacagatc ttctttccag
      541 gcttcgatca gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagagcaga
      601 tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg catcaccgtg aataatggtc
      661 ttagcttcat taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg
      721 acacctataa aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc
      781 taacctgaat tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat
      841 gcccatcatc agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa
      901 caggggctgt tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt
      961 cagttgagca aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt
     1021 tacctgatcc tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc
     1081 gaacatagtt ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca
     1141 acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat
     1201 ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg
     1261 ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg
     1321 ccaaaggcag tgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga
     1381 tccagggtct tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga
     1441 gcacagctct ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa
     1501 ctttctgctt aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc
     1561 gtgtacggcc atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct
     1621 cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt
     1681 ccactgcatt gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg
     1741 ctgaacctgc gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa
     1801 tctgggcacc gctttccacg ttaatacgtg ctgcatctgc catttctttc ggagcgctac
     1861 cagcaatttg cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt
     1921 tgtcgctttc ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc
     1981 ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga
     2041 tcaggcgatt tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt
     2101 gactgcaagg cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc
     2161 aatcctctga gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca
     2221 taaaaatcat catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag
     2281 ttctcaattt ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataatcggca
     2341 aatttacgtg ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc
     2401 gaaggtcaat tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc
     2461 agtgcgaagc tatcggcata agcttcacaa acgctctctg cctggcttgc cagaataccg
     2521 gaaaggccca gcaaaccgcc tgaaactggc aggacgctga ttaacggtgc cagttcacgt
     2581 aatgggcctg caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct
     2641 ttcggtaagt agagttccag acggtcagaa acgccattac gttcggcgtt atcgcggctg
     2701 gcctgaatcg cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc
     2761 gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt accggttaaa
     2821 tcgaggctgt cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac
     2881 gccagccctg gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta
     2941 gggcagatcc acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc
     3001 tcccagtctt tatcttctag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga
     3061 tggttttcca gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc
     3121 acatcggtgt cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg
     3181 tgggtatcct gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct
     3241 tccgcgttcg cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt
     3301 atttatcagt agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca
     3361 ggaaagccag caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa
     3421 tattcagcgt cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc
     3481 ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt
     3541 ccagcccacc gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg
     3601 caagcagcag caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg
     3661 tctcgttttg catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg
     3721 aactttgcag cagttgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa
     3781 agatcccggc agcaaaaggt ggcagcactt ttaccattaa cgtcgggatc accagatctg
     3841 gtacggtgag atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca
     3901 tcaggattgc gactataatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt
     3961 tataagagat acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta
     4021 cccagaacga cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca
     4081 gttgcggatc gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta
     4141 ccacgccaat aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt
     4201 tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa
     4261 tcagcagccc ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga
     4321 taaactgcac ggtcatcgca ccaacgaacg caaccagcaa actcaaactc gccagccaca
     4381 ccagaagacg actctggtag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc
     4441 ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag tgaaagccag acagcaggaa
     4501 gctgaatcat cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc
     4561 cgataaacga actggcactg atataggtcg cggtgagagt catcgccagc acaataccgc
     4621 ccatagagcg gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca
     4681 tcgcataaac cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt
     4741 caagctgcat cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag
     4801 cacagtccaa taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag
     4861 ccggtaaaac cgggggcaac gccaggtaag taagcgtcta ctaaccaaac tgccagatac
     4921 aaaagggtca gccccagcgc ccagcgggcc tctttatggg cctgaacaaa acgagtgtcc
     4981 attttttgtc cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc
     5041 ccagtaataa aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc
     5101 tgaagaccga gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg
     5161 tcattcatga tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc
     5221 tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc
     5281 ttaccgatca ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc
     5341 caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg
     5401 aaggtgttcg gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact
     5461 tcttcttgct tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg
     5521 tcaacgccgg tgatcatttc tgtaaccggg tgttctacct gaatacgggt gttcatttcg
     5581 atgaaataga actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg
     5641 atatcaacac acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg
     5701 cccggtgctg gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt
     5761 tccgccagat agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga
     5821 ggattctcca ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc
     5881 gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg
     5941 cgaccgccgc cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg
     6001 gcgcggtttt tatccatatc gtcgcccagc gggccgtcag aaccaggtac gcaaggaacg
     6061 cccgcttttt tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct
     6121 gctttcgggc cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc
     6181 tcggagagga agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg
     6241 atgattgccg ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt
     6301 tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc tggagtgcac agcgacagtc
     6361 ttgatgccca gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca
     6421 ataacaattt tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa
     6481 ttctaccggt tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc
     6541 gatctggttc atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt
     6601 ctgacccact tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac
     6661 catcggggaa cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg
     6721 aacggtcgcc ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta
     6781 agcttgttgc atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc
     6841 ttcgccttca gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt
     6901 tttaatctta cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca
     6961 ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt
     7021 ccagcaggcc gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac
     7081 attgtgcagc gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca
     7141 atgtcacctt ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac
     7201 gatttcgtag caattggcag ctaaatactg gtcttatcag ggaatataat caacagctaa
     7261 catgtaaata accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag
     7321 cgccatcatc gacggaactt cttataacgt aaggtgaaaa gctgaaaagc cagccctgcg
     7381 aagatgaggg gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg
     7441 atcgcgacaa gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc
     7501 atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca accaactgac aattctcagc
     7561 attaaatagg ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg
     7621 taccagaaat cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa
     7681 caggcgacgg aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg
     7741 tggtcagtcg aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg
     7801 tgttcgacga aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc
     7861 aaaatgagtt ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg
     7921 cgaccctgga tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat
     7981 atctcttttg ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag
     8041 gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta
     8101 cgcagaataa atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc
     8161 gccacgcagc cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca
     8221 gtgtcaattg ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca
     8281 cgagggagaa taccgctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg
     8341 gaaacggcaa cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg
     8401 ccaccactgg cacccgtcac gacaatctca ccgtcctgcg ggcgaacacc ggcatcttcc
     8461 agcgccatca cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc
     8521 gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc
     8581 agcccacccc agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca
     8641 tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc
     8701 ggaaaattac ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg
     8761 ctcgaccagt gaacatcgac cgtgacatcg ccctccggca ggcgacgttc gtccagagtc
     8821 tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt
     8881 cctcatgtgc atgatggatt ggaaaataat ttctgaagac tatactcgct aatggaaaag
     8941 caaagccgat gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc
     9001 ctcactatcg gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg
     9061 ctgctcaccg ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac
     9121 gccattcagt ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac
     9181 cttgtgtcga atgacttcag cgcattaagg ccacaaatta ccgaattaat ggtgtcagca
     9241 gatatcgttc gtgtagacct gctgcatggt gataaacagg tttataccct ggccagaaat
     9301 ggtagttatc gtccggttgg caccagcgat ctgtttcgtg aactgagcgt tccgttgata
     9361 aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc cgatgggcaa ctatttccat
     9421 tcgttgatga ccaccgcgcc gctcacgggg gcgattggct ttatcattct tatgctcttt
     9481 ctggcggtac gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct
     9541 actcgtatct taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg
     9601 cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa
     9661 cacagccgtc ttgatacgct gatccgctct tatgccgcac aggacgtgaa aaccggcctc
     9721 aataaccgac tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta
     9781 ggtacccacg ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatacc
     9841 tgggggcaca gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt
     9901 atgatgcgct accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg
     9961 ttaccgcacc ggacgttaaa agaggcagag agcatagcca gtcagttaat caaagccgtc
    10021 gataccttgc cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgt
    10081 gcctggcgta gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt
    10141 aatgcgggat tgcagggtgg caatagctgg gctatttatg atgactcgtt gcctgaaaaa
    10201 ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg
    10261 cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg
    10321 tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg
    10381 cagtttggct tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg
    10441 cgttactggc cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgtccg
    10501 cgttttcagc gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc
    10561 ataattattg aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt
    10621 attcgtttag tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg
    10681 gtaagcacca gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg
    10741 gtcaggaaca ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc
    10801 tgctccggga ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag
    10861 accctgattc agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca
    10921 cttgatacta acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc
    10981 gttttcacgt agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga
    11041 ttgttgcagc acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct
    11101 gctgatgcat taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc
    11161 actaattttc accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa
    11221 agtaagcgga ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa
    11281 atttcgtggc atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat
    11341 ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga
    11401 tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg
    11461 ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt
    11521 cttcgtgact gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat
    11581 gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg
    11641 cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc
    11701 gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt
    11761 tgatatcggt ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc
    11821 ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg
    11881 taattacggt tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc
    11941 ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg
    12001 tgttccacgc ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct
    12061 gaccggtatt gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc
    12121 cgacatctcc gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga
    12181 ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg
    12241 tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt
    12301 cagcgaagag taatcggatg caggcagggg aagcgtctgt ttaccctgcc tggtctgata
    12361 cgagaatacg cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc
    12421 cttattctgg cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg
    12481 ttcagtcaaa tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat
    12541 gctcctcgtg aattgctgga tggcatatcg cagacgctgg cctcgcgtga ccaactagaa
    12601 cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt
    12661 ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag
    12721 gatgagcaga aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa
    12781 gttgttatcg ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac
    12841 aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt
    12901 tgtgatgcga cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca
    12961 gccggtaacg gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt
    13021 cgtgttggtg atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg
    13081 gtcgcggttg tctcttccgt aaaactcgat acccagcgcg cttatactgt gatccaggcg
    13141 cgtccgactg caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt
    13201 aacggcgcta acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg
    13261 cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg
    13321 gcaacgggga tcgctcagcc gactccgcag caaccggcga caggaaatgc agctactgcg
    13381 cctgttgcgc cgacacagcc cgctgctaat cgctctccac aaagggctac gccgccgcaa
    13441 agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc
    13501 agggacgctg ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct
    13561 ggccggataa cctgattgtt ttccggccaa actgggtgtt acttatcttg ttgtattgga
    13621 tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg
    13681 atctgatcag cggctcgatg cttggcgtac gcgtattggc gatgagcatc attgcttacc
    13741 tggtggcgct gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg
    13801 tcatgttgct ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta
    13861 acgtctcttt cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt
    13921 ggattttctt gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat
    13981 gacttctctg tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg
    14041 cgtgaccttt gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc
    14101 gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag gcgggtgtcg cgcaaacggc
    14161 acaggatctc ccggtgctgg gtgcggatat tatcgttatc ctgaacggag aagtgctcga
    14221 gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca
    14281 tcaggtgatg acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt
    14341 caccgatgtg actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg
    14401 tgaaccgtta gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag
    14461 gaagataaat ggcagctatc acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt
    14521 attaagtaat tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag
    14581 taaacgtaac gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa
    14641 ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa
    14701 gtcgtgtact tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt
    14761 ttcttcatgc atccgacatc atgccgcata ccgaatgtgt ggcgggtgaa gaacaaaagc
    14821 aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg
    14881 tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt
    14941 ctcgctatct ggtatttatg ccaggggctt ctcacgttgg ggtttcccaa cgaattgaaa
    15001 gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg
    15061 ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg
    15121 cttatctgaa acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt
    15181 atcagctgta cggcgaactg gcgctggcgc agcgtgttct gcgtgatttt gccgacgccg
    15241 aactggaccg cattcgcgtt gactcacgcc tgacttacga agcattgctg gagtttacct
    15301 cggagtacat tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt
    15361 tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt ggaacgcaaa gtagaactga
    15421 aatccggtgg ttatctgatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata
    15481 ccggagcgtt tgtcggtcat cgcaatctgg acgacactat tttcaatacc aatattgaag
    15541 cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg
    15601 atttcatcga tatgaataat gaagatcacc gccgccgcgt gctgcattcg ctggagcagg
    15661 cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg
    15721 agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa
    15781 cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg
    15841 agattgttcg tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg
    15901 cagtagccga agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg
    15961 ttggcaaaca ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg
    16021 taatgatgta aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta
    16081 gcagacaagg agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg
    16141 ctcgttgtga tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttaac
    16201 gcctggcgtc cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc
    16261 agtcagctct cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt
    16321 gcagaactaa aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc
    16381 tggcagagcc tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc
    16441 tttcgcacca acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac
    16501 atcagcgatc tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt
    16561 tttctgacgc catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac
    16621 gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac
    16681 ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc
    16741 gtctggctac aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac
    16801 aatattgctc tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc
    16861 gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag
    16921 caaacgcata cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc
    16981 tggcagttct ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga
    17041 gcattgacgc tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt
    17101 gacgaactcc ggattcgcgc tagtaatctg gagctggcag gcctggaggg catacgcccg
    17161 ctggccgcga aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc
    17221 aagattaaca ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag
    17281 gcatcgtgga gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc
    17341 tccgggacgc tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca
    17401 aagatgcctt acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact
    17461 ataagctggc tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa
    17521 gccaaagccg tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc
    17581 tggctgggta ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc
    17641 ccggaaaact tgatgggtaa agacctggtt gattatttaa gtggtgcgat tcagggcggt
    17701 gaagcggata acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac
    17761 aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg
    17821 gactggcctg cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg
    17881 atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc
    17941 cctgactact caaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc
    18001 gttggccctt actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa
    18061 ctccagctcg acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgaa
    18121 ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc
    18181 gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt
    18241 gaaccactga cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa
    18301 ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc
    18361 ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa
    18421 gtgggcattg atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat
    18481 ctgaataatg tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta
    18541 gcggtaaacg ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt
    18601 gcggataacc atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct
    18661 atttgggcgg cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactt
    18721 aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag
    18781 agtgtcggtg gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtca
    18841 ctgggaaatc agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc
    18901 ctggttgagg cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg
    18961 tggctggcga atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat
    19021 tcaacgccgt catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac
    19081 gcacaaatac gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt
    19141 gatatcacca tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc
    19201 tcgcggctta ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg
    19261 aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg
    19321 cccatacgcc agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg
    19381 cagccagatg aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt
    19441 accgaaatca ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg
    19501 atgcgtaagc tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc
    19561 attcgcagca ccgcgtggat taaagacggc gttatgcaca ccgacgacac gctggtggat
    19621 ggcctggagg cggatatcgc catgaaaggg tcggtaaatc tggtacgccg cgacctgaat
    19681 atggaagcgg ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg
    19741 gttaacccca ttgttggcgc ggcagtgttt gccgccagta aagtgctggg gccgctgtgg
    19801 agcaaagtct ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac
    19861 gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg
    19921 ccccaatctc atatgataat cgttgccaaa ggccaacgag ccggaacata actgtaggcc
    19981 ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac
    20041 gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacggcc
    20101 tgaaacatca ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg
    20161 gcgatctcta ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta
    20221 aagatggttc ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa
    20281 ccggatttgc ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg
    20341 cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc
    20401 atagcccgtt gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg
    20461 atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga
    20521 ctgccagcct cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag
    20581 cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca
    20641 aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg
    20701 atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg
    20761 tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt
    20821 ggccgggcgt gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc
    20881 gtggcacttc agtatttagt ggacaggtcg gggagctggt ggcttcagaa ctgtgtaccg
    20941 tggttgatga cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gatgaaggta
    21001 cgccaggcca gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata
    21061 aactcaacgc gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg
    21121 cccatctgcc catgccgcgt atgaccaaca cctatatgct gccgggtaaa tcgaccccgc
    21181 aagaaattat tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg
    21241 tggatatcac ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg
    21301 gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc
    21361 agcagatttc gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta
    21421 aagaagggca aagtttgccg gttggcgtgg gccagccaac gttgaaagtc gataacctga
    21481 ctgttggcgg tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg
    21541 tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt
    21601 ttgtagaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg
    21661 aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt
    21721 cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata
    21781 acgcggcagc aggatctcca gctccccacg attgatctcg ttgatcaccc acatcagcgg
    21841 cacgtaggcg atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt
    21901 cacaaatctc ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa
    21961 ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt
    22021 ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc
    22081 gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc
    22141 cagaccgtcg gcaatcaggt cgggggctgg aattccggta accagattgg cgctcaaacc
    22201 tgggtattct ttcagcattt tggctgtcag cccggcgaga acattttgtg ccatagttga
    22261 agaacagcca atgcgtagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg
    22321 aacatcctgc acttcatgaa gcatacgacg gcagccctgg tagtaaattc taccggcttc
    22381 ggtcaggcca atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag
    22441 ttttgacacc gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt
    22501 aaaagagcca aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat
    22561 tcactctgac ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc
    22621 taatatatta aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct
    22681 ctgcggcgac agatgctgaa aataataacg cctgctctct ctatacaacc aaggtcaaca
    22741 tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat
    22801 tgcttttatc actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct
    22861 acgactttgt ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt
    22921 tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt
    22981 acggccatca cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc
    23041 tattacaccg aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc
    23101 gcgccggacg tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa
    23161 aaaggacaga tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg
    23221 caagccgatg ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt
    23281 cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta
    23341 ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg
    23401 gatcttgaac gcacggtgat ccgagcgcca gcagatggct gggtgaccaa cctcaacgtc
    23461 tataccggtg agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc
    23521 ttctatgtac tggcctatat ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt
    23581 gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc
    23641 gctggggtca ccaacgccag cagcacgcgt gacgacaaag ggatggcgac catcgactct
    23701 gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc gtattcgtct cgacaaccag
    23761 caagagaaca tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat
    23821 cgcgacgaaa gccaggattc gttcttccgt aaaatggccc atcgcctgcg tgagtttggt
    23881 taatcacgat gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg
    23941 cgaccgccat tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct
    24001 gggcggtact gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac
    24061 cgtattctgg cgctattcgc tatcgtggct ttttgcgcat catcggcaca tttattggct
    24121 gtattgccgg actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg
    24181 tgtgctgtat ctgggccggt ttttgtacct ggatatcctc gctggtacga atagaaaact
    24241 cgtacgcgtg ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg
    24301 aaccattgct tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg
    24361 tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc
    24421 gagagctgga aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg
    24481 gcgatggtga agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcactac
    24541 aaggcatgcg cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt
    24601 taaaagcgat caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta
    24661 ttcagaatac gcgcccggaa ttgatcactg atactttccg cgaatttttt gacacgccgg
    24721 tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga
    24781 ctggggaacg ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc
    24841 agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc
    24901 tgcaaggcga accggaagta aaagtagagt cagccgaacg tcatcatgca atggttaact
    24961 tctggcgaac cacactttcc tgcattctgg gcacgctttt ctggctgtgg acgggctgga
    25021 cttccggcag tggtgcaatg gtgatgattg cggtcgtgac gtcactggca atgcgtttgc
    25081 cgaatccacg catggtggcg atcgacttta tctatgggac gctggccgcg ctgccgttag
    25141 ggctgctcta ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta
    25201 ttagcctggc agtgctggga ttcttcctcg gtatagaagt acagaaacgg cgactgggct
    25261 cgatgggggc actggccagc accataaata ttatcgtgct ggataacccg atgactttcc
    25321 atttcagtca gtttctcgac agcgcattag gacaaatcgt cggctgtgtg ctcgcgttca
    25381 ccgttatttt gctggtgcgg gataaatcgc gcgacaggac tggacgtgta ctgcttaatc
    25441 agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc
    25501 acctcccggc actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc
    25561 cgaaatttcg cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga
    25621 tcccggttaa cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg
    25681 tgatatctgc ccgtagcgat gataaacgtc gtcggtactt tggccagttg ctggaagaac
    25741 tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg
    25801 ttcatcggct ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca
    25861 aaaccgacgc caaaagcgtc ggttttttca tggctatact tagcgatgca cggcagaact
    25921 cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg cctttgattt tgatgagatt
    25981 gagagtcagg aggattttta tcgtgacttt agccaaacct ttggtctggc taaagataag
    26041 gtacgcgatc tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt
    26101 gagattgaat ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg
    26161 ctgtttgatg aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag
    26221 cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga caaaacgatg agggtttatt
    26281 tgtacagttc agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg
    26341 ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc acgtatatcc attggcgaag
    26401 acgccacacc acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagcat
    26461 caatggagtc ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcta
    26521 cagtggtttt gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt
    26581 cgtgacccat atcacaaaat caagtataca ctaattattg aactaattaa taccacacta
    26641 actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt aataacaaaa aagtataaaa
    26701 aacacggtta tttattatta attatagtat tttcaattag ataatgaatt ttgcataatg
    26761 tgcttttttt accgtatgta ttcattttgt gaatgatgtg tcgcggtaaa acgcactatt
    26821 cgttaaatgt atcgcaggag aaagaagggg aatgagaggg ataagcaaca ttttccccgc
    26881 cgccagaagc gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg
    26941 tacaggtctt tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg
    27001 atggtgccca gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc
    27061 gccgcgccag ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc
    27121 agattcttca atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc
    27181 atttccattt tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact
    27241 ttagactgat taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg
    27301 gagctaaatt tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta
    27361 gccgagcttc gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga
    27421 tgaattttta tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg
    27481 gattttatca aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgttt
    27541 aaaagtaaat taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt
    27601 caccacctgt tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt
    27661 cctgattacg gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat
    27721 gtggtcattg taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt
    27781 agtttatcaa tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg
    27841 tattggccag gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc
    27901 tctgtatgat atcgctccag tgactcccgg tgtggccgtc gatctgagcc atatccctac
    27961 tgctgtgaaa atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga
    28021 tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt atggatcgtt ccgacctgtt
    28081 taacgttaac gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa actgcccgaa
    28141 agcgtgcatt ggtattatca ctaacccggt taacaccaca gttgcaattg ctgctgaagt
    28201 gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggatat
    28261 cattcgttcc aacacctttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt
    28321 gccggttatt ggcggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg
    28381 cgttagtttt accgagcagg aagtggctga tctgaccaaa cgtatccaga acgcgggtac
    28441 tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc
    28501 tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg
    28561 tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg
    28621 taaaaacggc gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagaacgc
    28681 gctggaaggt atgctggata cgctcaagaa agatatcgcc ctgggcaaag agttcgttaa
    28741 taagtaattg attagcgaat aataaaaaac cggagcacag actccggttt ttgttttgag
    28801 cactcgactt aattggttgc cggatattcc tgaatggtga cctgcagcgt taactgctta
    28861 tcatcacgca tcactacaac cgggatcacc gaaccagggc gaatttccgc cacctgatcc
    28921 atcgtctcca gagcagagat ggccggtttg ttatccaccg aaataatcag atcgttgacc
    28981 tgaatacccg cattcgccgc tgggccgtca ggtgacactt cattaaccac gatcccttgc
    29041 agttgatcta taccaccgct ctgcgcgtgc agtggtgcga tctcgcgtcc gccgatacca
    29101 atgtagccgc ggatcacgcg accatcgcgg atcagcttat ccataatttt ggttgctaac
    29161 tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc catcgttact cttatcaaac
    29221 gacagcgtgt taatgcccat cagttctccc agcgagttca ccagcgcgcc gccagagtta
    29281 ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc gcccggtcgg gttcagaccg
    29341 attcgacccg tggcactaat aatcccttgg gtaatggtct gcccgaggtt gtacgggtta
    29401 ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac gtgcattaat tggaatggta
    29461 ggtaaaccgc cagtggcatt aattttaagt accgccagat cggttagaga gtcagatccc
    29521 accagcaatg cttcaaatac acgtccatcc tgtaaggcga cgatgatctg atcggcgtcg
    29581 ttgatgacgt gtttattggt gatgatataa ccgcgttgat ccatgattac accggatccc
    29641 agggtgcgga tctcaagctg gttgtgagag ttggtgttca aaccacggtt gtaaacgtta
    29701 accaccgctg gcgcggcgcg gcgaaccgcc agattatagc tggcaggcgt ctcatcggta
    29761 ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca gcgaaggcat ggcaaccagc
    29821 agaatagcgc cgacaattaa tccaatcgca acggaacgta agagcttcac aaacatgatg
    29881 gaggcgtcat taaaaaaggg aacggcagca gcataccacg agttaaccgg acatcacacg
    29941 tcagcctgat gcccggttta cgacattaac gcatcagcag atagatgctt tcattgccgc
    30001 gtacaatttg cagggcgatg atggccggtt ttgccgccag cactttacgc atttcagcaa
    30061 tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc gtctttttgc aagccagcct
    30121 gagcagctgg gcttcctttg acaacttcat ctatcttaat acctttgccg ccatctttta
    30181 gctgaccatc gctcaacgtt gcaccttcca gcgctggccg acgaagaggt gctggtatca
    30241 agcgtcactt ctacttccag tggtttgccg ttacgcagca ggccaagctt cactttcgtg
    30301 cccggctctg tagtcgcgat acgagagcgc aactcagcaa agctattcag cggtttgccg
    30361 ttgaggctgg taataatatc gcccgctttg acgcccgctt tcgctgagcc agaacttggc
    30421 aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg
    30481 gcactcatct cggtgccttt gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata
    30541 agctgctgcg ccagtgttcg cgccatatta ctggggatgg caaatccaat cccgacgctc
    30601 ccgccgccag gcgcaaggat tgcagtgttg atgccaatta actcaccgtt aaggtttaat
    30661 agtgcaccgc cggagttacc gcggttaatg gaagcatctg tctggataaa gttttccaga
    30721 ccttcaagat tcaacccgct gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc
    30781 ccaaggccaa atgggttacc gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag
    30841 tcggcaatag cgatttgcgt taatttgctc gggttttgaa tttgtaacag ggcgatatcg
    30901 ctctggtcat cgctaccaat cagttttgca tcaaactcgc gcccatcatt gagctgaata
    30961 ctgattttct gtgcctgatt aatcacatgg ttgttggtca gcacatagcc tttactggcg
    31021 ttgatgatga caccggaacc taaaccttcg aagggttgtg caggttgatc cggtaaatca
    31081 tcaccaaaaa actttttgaa ttcttccggg attttctgtc cctgactggc cgttccttcc
    31141 acccgtacgc tcaccactgc cggaagcact ttttccagca ttggagccag actggggaga
    31201 ggggcctgat cggcaacctg gcctggaatc gacgcgacgg cctgaaatga cgccgagaga
    31261 gttaacccgg cacttaacgc taatgcactc aacagctggg tttgtttttt cattattcct
    31321 gctctcgtac ctgaatgata agaaaagaga ttcaaaacag tttgatgtta ttgaattttc
    31381 agacggctaa caatgagaca ccagattaaa tgatagtcgg ggagggagag aagaggaggg
    31441 cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg cgccagtacg cagcaggccg
    31501 gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg cctgatcgtt gctggcttca
    31561 gactctgcca gacgattacg gaacgggttt gcttcagcag acagttccgg cagcaggctg
    31621 ctggagcttt ttgccatgtg ctgatacagc tggcgatagt cgtgcgccat ggtatccagt
    31681 aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc gatactcgtc cagttcagct
    31741 ttattctttt ccagttcgta ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa
    31801 cgcatggcca cagcaccaat aatgatgccg acgactaacc caattagcgc atattcccag
    31861 gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg ttggcttcag gctccatgcc
    31921 tgcggctgat tatgccacta taaccgttaa ttccacagaa gtggaatccc gactgcatat
    31981 cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac aacggcgcac cgattattca
    32041 aggaataaca ataagatcat gcaaagcgtt accccaacat cgcaatacct gaaggcgctc
    32101 aatgaaggca gccatcaacc cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt
    32161 atttatcaag aactcatcaa tagcaggcca ccagccccca ggacgagtgg gctaatggcg
    32221 cgggtcggta agctgtgggg taaacgcgaa gacacaaagc atacgccagt gcgtggctta
    32281 tatatgtggg gcggtgtagg acgcgggaaa acctggctga tggacctttt ctatcaaagc
    32341 ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa
    32401 gagctaactg ccttacaggg gcagaccgat ccgctggaaa ttattgccga tcgctttaaa
    32461 gccgaaactg acgtgctctg ttttgacgaa ttttttgttt ctgatattac cgacgccatg
    32521 ctacttggcg gtctgatgaa agccctgttc acccgcggta ttaccctggt agcgacgtca
    32581 aatattccgc cggacgaact ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca
    32641 atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg acgctggtgt tgattatcga
    32701 ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac tcaacgatga aacccgggcg
    32761 cagatggata aactatggtt ggcgctggcg ggggtgaaac gagaaaattc accgacgtta
    32821 gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga accagacgct ggcggtttct
    32881 tttactacgc tgtgcgtcga cgcccgcagt cagcatgact atattgcgct ctcacgcctg
    32941 tttcacacag taatgttgtt tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg
    33001 cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt
    33061 gcagaagtgc cgctgtatga aatttatcag ggtgagcggc tgaagtttga gttccagcgt
    33121 tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg
    33181 ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt tgaccccgac ttctctataa
    33241 tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa ctttttgtgt gctggcatag
    33301 gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt
    33361 ttgggtgttc accaacgtgt aactatttat tgggtaagct tttaatgaaa acttttacag
    33421 ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga cgcgaccggt aaaactctgg
    33481 gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa gcacaaagcg gaatatactc
    33541 cgcacgtaga taccggtgat tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg
    33601 gcaacaagcg tactgacaaa gtgtactatc accacaccgg ccacatcggt ggtatcaaac
    33661 aagcgacctt tgaagagatg attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta
    33721 aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg
    33781 cgggtaacga gcacaaccac gcggcacagc aaccgcaagt tcttgacatc taatcgggat
    33841 tataggcaat ggctgaaaat caatactacg gcactggtcg ccgcaaaagt tccgcagctc
    33901 gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt
    33961 acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg
    34021 ttgagaaact ggacctgtac atcaccgtta aaggtggtgg tatctctggt caggctggtg
    34081 cgatccgtca cggtatcacc cgcgctctga tggaatacga cgagtccctg cgttctgaac
    34141 tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc
    34201 tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta attggcttct gctacggcag
    34261 aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt atggctaaat tctgaatcag
    34321 cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac
    34381 atcgccaata agggtctaag tcaactattt cagactaaag cgcatctctt tttccccatt
    34441 tccggcatcc actcaccaca aaggtcgcaa aatctggtaa actatcatcc aattttctgc
    34501 ccaaatgtcg ggtattgctc attttttgtt tgattttcga acaaagagag tcgttcctta
    34561 ttgggtaaca caacttctga ctggccacct ggtggctggt agcagtaaaa attctgacta
    34621 tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt cggtaatgac gctgttttcc
    34681 ggtcctactg acatctatag ccatcaggtc cgcattgtgc tggctgagaa aggtgtaagt
    34741 ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg atctgattga cctcaacccg
    34801 aatcagagcg ttccgaccct ggtggatcgt gagctgaccc tgtgggaatc tcgcatcatt
    34861 atggaatatc tggatgagcg tttcccgcat ccgccactga tgcctgttta cccggtagct
    34921 cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag actggtacac gctgatgaac
    34981 actatcatca acggttcagc ttctgaagca gatgccgcac gtaagcaact gcgcgaagaa
    35041 ctgctggcga ttgcgccggt cttcggtcag aagccgtact tcctgagcga tgagttcagc
    35101 ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc cgcaactggg catcgagttc
    35161 agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc gcgtctttga gcgtgactct
    35221 ttccttgctt ctttaactga agcagaacgt gaaatgcgtc tgggccggag ttaatctgta
    35281 tggatttgtc acagctaaca ccacgtcgtc cctatctgct gcgtgcattc tatgagtggt
    35341 tgctggataa ccagctcacg ccgcacctgg tggtggatgt gacgctccct ggcgtgcagg
    35401 ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa cattgcgccg cgtgctgtcg
    35461 gcaatctgga actggcgaat gatgaggtgc gctttaacgc gcgctttggt ggcattccgc
    35521 gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta cgcccgtgaa aatggcgcag
    35581 gcacgatgtt tgagcctgaa gctgcctacg atgaagatac cagcatcatg aatgatgaag
    35641 aggcatcggc agacaacgaa accgttatgt cggttattga tggcgacaag ccagatcacg
    35701 atgatgacac tcatcctgac gatgaacctc cgcagccacc acgcggtggt cgaccggcat
    35761 tacgcgttgt gaagtaatac aaaacaggcc caggcggcct gttttgtctt tttaatgact
    35821 taataaaata gcgtgattgt ggcaatcata ttcaccacga aaccgactgc catggggatc
    35881 gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa ttcctgacgt aacgaccacc
    35941 accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg caaaatgcat cggcataatc
    36001 attacaaccg ctggtacatg tagtccggct gcgatattcg gaataagact ggcaaatggc
    36061 ataaacggcg cattgccaga gcccatcaca atggcacaaa tggcaatgac tagcgccata
    36121 ataatcatca cgccaatacc gcccagacca gaatgctccg cacccctgat aaccgcatca
    36181 acagtgccaa tcgtcgttaa gcctttcgca aatatttcac ccgcgaccac cagcgttacc
    36241 acgttgacaa actgcgtacc catgccgtca aaaaacgcct gcacatcgtc cattgtttcg
    36301 cgcaagttat gcttgcggaa gaactcaaca aacatcgtca ccgtcaaact tagtaacatc
    36361 accaccacca gatgcagttc tgactgcatc agcccgacgt gggcgaggaa cagcgagccg
    36421 agcatcagga ttaacggcat cacaggtaaa atggcgtaat agagcggcgg gacattatcg
    36481 agagtttttt gctctgcctg ttcgtgattg atatttttat cttttttgtc aaaagcgcgt
    36541 tgcacgaaaa agtgggagat cgccaccgag ataatgacgc aagaggcgac cggaagctgg
    36601 tagtggaaga agtatgtggc aattttcatt cccgcgacct gggctgcaaa aatggagttc
    36661 gtttccagaa tcccccactc aatggacatc gtggttgcga taaccgccac cgcagagagg
    36721 cgacttactc ccagactcac cagcgtcgga aataaggtga ccatcagcaa catacccaga
    36781 ccggaggcgc tggtaataaa ctgcgccatg atttggccga tgacgtaagt tgccgacaga
    36841 ataatatacg gcgagcgaat gagttttaac gggcggctta gcaagctcac catcgcgcaa
    36901 ctggctccta tgcgctccat gtagcgggca taaccgccca ccgccataat cgacagcccc
    36961 aatccggcaa tgcggttgga cagcatgcgc aggatttcat tataaatgtc gacgatcaag
    37021 tagccgctgc ttttggcggg atcgagtaca ccaccgaacc ctaaccagac tccgcacatc
    37081 atcaggaaga cacctgcggc agccagcacc acctgaggtt tgtagttttt caggatcaaa
    37141 tagcccatcg taattaatac gatgacagat ataattat
//
LOCUS       DAJVPT010000029        36796 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVPT010000029 DAJVPT010000000
VERSION     DAJVPT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..113)
                     /locus_tag="OQM47_001423"
     CDS             complement(<1..113)
                     /locus_tag="OQM47_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCS1479637.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACV"
     gene            396..437
                     /gene="hisL"
                     /locus_tag="OQM47_001424"
     CDS             396..437
                     /gene="hisL"
                     /locus_tag="OQM47_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1479638.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    413..536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            583..1482
                     /gene="hisG"
                     /locus_tag="OQM47_001425"
     CDS             583..1482
                     /gene="hisG"
                     /locus_tag="OQM47_001425"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1479639.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1488..2792
                     /gene="hisD"
                     /locus_tag="OQM47_001426"
     CDS             1488..2792
                     /gene="hisD"
                     /locus_tag="OQM47_001426"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1479640.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2789..3859
                     /gene="hisC"
                     /locus_tag="OQM47_001427"
     CDS             2789..3859
                     /gene="hisC"
                     /locus_tag="OQM47_001427"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1479641.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3859..4926
                     /gene="hisB"
                     /locus_tag="OQM47_001428"
     CDS             3859..4926
                     /gene="hisB"
                     /locus_tag="OQM47_001428"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1479642.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4926..5516
                     /gene="hisH"
                     /locus_tag="OQM47_001429"
     CDS             4926..5516
                     /gene="hisH"
                     /locus_tag="OQM47_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1479643.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5516..6253
                     /gene="hisA"
                     /locus_tag="OQM47_001430"
     CDS             5516..6253
                     /gene="hisA"
                     /locus_tag="OQM47_001430"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1479644.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6235..7011
                     /gene="hisF"
                     /locus_tag="OQM47_001431"
     CDS             6235..7011
                     /gene="hisF"
                     /locus_tag="OQM47_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1479645.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7005..7616
                     /locus_tag="OQM47_001432"
     CDS             7005..7616
                     /locus_tag="OQM47_001432"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1479646.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7802..8779)
                     /gene="wzzB"
                     /locus_tag="OQM47_001433"
     CDS             complement(7802..8779)
                     /gene="wzzB"
                     /locus_tag="OQM47_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1479647.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8925..10045)
                     /gene="ugd"
                     /locus_tag="OQM47_001434"
                     /pseudo
     CDS             complement(8925..10045)
                     /gene="ugd"
                     /locus_tag="OQM47_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10295..11701)
                     /gene="gndA"
                     /locus_tag="OQM47_001435"
     CDS             complement(10295..11701)
                     /gene="gndA"
                     /locus_tag="OQM47_001435"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1479648.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11810..12169)
                     /locus_tag="OQM47_001436"
     CDS             complement(11810..12169)
                     /locus_tag="OQM47_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479649.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12819..13382)
                     /locus_tag="OQM47_001437"
     CDS             complement(12819..13382)
                     /locus_tag="OQM47_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479650.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13589..14527)
                     /locus_tag="OQM47_001438"
                     /pseudo
     CDS             complement(13589..14527)
                     /locus_tag="OQM47_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14590..14964)
                     /locus_tag="OQM47_001439"
     CDS             complement(14590..14964)
                     /locus_tag="OQM47_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCS1479651.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14968..16116)
                     /gene="wzy"
                     /locus_tag="OQM47_001440"
     CDS             complement(14968..16116)
                     /gene="wzy"
                     /locus_tag="OQM47_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1479652.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16136..17041)
                     /gene="rfbG"
                     /locus_tag="OQM47_001441"
     CDS             complement(16136..17041)
                     /gene="rfbG"
                     /locus_tag="OQM47_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1479653.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17034..17924)
                     /locus_tag="OQM47_001442"
     CDS             complement(17034..17924)
                     /locus_tag="OQM47_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1479654.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17954..19210)
                     /gene="wzx"
                     /locus_tag="OQM47_001443"
     CDS             complement(17954..19210)
                     /gene="wzx"
                     /locus_tag="OQM47_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1479655.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19226..19771)
                     /gene="rfbC"
                     /locus_tag="OQM47_001444"
     CDS             complement(19226..19771)
                     /gene="rfbC"
                     /locus_tag="OQM47_001444"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1479656.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19776..20654)
                     /gene="rfbA"
                     /locus_tag="OQM47_001445"
     CDS             complement(19776..20654)
                     /gene="rfbA"
                     /locus_tag="OQM47_001445"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1479657.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20713..21612)
                     /gene="rfbD"
                     /locus_tag="OQM47_001446"
     CDS             complement(20713..21612)
                     /gene="rfbD"
                     /locus_tag="OQM47_001446"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1479658.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21612..22697)
                     /gene="rfbB"
                     /locus_tag="OQM47_001447"
     CDS             complement(21612..22697)
                     /gene="rfbB"
                     /locus_tag="OQM47_001447"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1479659.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23070..23963)
                     /gene="galF"
                     /locus_tag="OQM47_001448"
     CDS             complement(23070..23963)
                     /gene="galF"
                     /locus_tag="OQM47_001448"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1479660.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24138..25532)
                     /gene="wcaM"
                     /locus_tag="OQM47_001449"
     CDS             complement(24138..25532)
                     /gene="wcaM"
                     /locus_tag="OQM47_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1479661.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25543..26763)
                     /gene="wcaL"
                     /locus_tag="OQM47_001450"
     CDS             complement(25543..26763)
                     /gene="wcaL"
                     /locus_tag="OQM47_001450"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1479662.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26760..28040)
                     /gene="wcaK"
                     /locus_tag="OQM47_001451"
     CDS             complement(26760..28040)
                     /gene="wcaK"
                     /locus_tag="OQM47_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1479663.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28220..29698)
                     /gene="wzxC"
                     /locus_tag="OQM47_001452"
     CDS             complement(28220..29698)
                     /gene="wzxC"
                     /locus_tag="OQM47_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1479664.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29700..31094)
                     /gene="wcaJ"
                     /locus_tag="OQM47_001453"
     CDS             complement(29700..31094)
                     /gene="wcaJ"
                     /locus_tag="OQM47_001453"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1479665.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31149..32519)
                     /gene="cpsG"
                     /locus_tag="OQM47_001454"
                     /gene_synonym="manB"
     CDS             complement(31149..32519)
                     /gene="cpsG"
                     /locus_tag="OQM47_001454"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1479666.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32712..34148)
                     /gene="cpsB"
                     /locus_tag="OQM47_001455"
     CDS             complement(32712..34148)
                     /gene="cpsB"
                     /locus_tag="OQM47_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1479667.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34151..35374)
                     /gene="wcaI"
                     /locus_tag="OQM47_001456"
     CDS             complement(34151..35374)
                     /gene="wcaI"
                     /locus_tag="OQM47_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1479668.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35371..35850)
                     /gene="gmm"
                     /locus_tag="OQM47_001457"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35371..35850)
                     /gene="gmm"
                     /locus_tag="OQM47_001457"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1479669.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35850..>36796)
                     /gene="fcl"
                     /locus_tag="OQM47_001458"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35850..>36796)
                     /gene="fcl"
                     /locus_tag="OQM47_001458"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1479670.1"
                     /translation="AGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDSRAVHDFFA
                     SERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKLMFLGSSCI
                     YPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRTVMPTNLYG
                     PHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMAAASIHVME
                     LAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASKPDGTPRKL
                     LDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
BASE COUNT         9901 a         9200 c         8236 g         9459 t
ORIGIN      
        1 acacaagcct ctccattctg acgagtaatg aggatcgggg catgatcttc aacggctttc
       61 atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg catgtaaatc
      121 tcctattttg tacagttcat tgtacaatga taggtgttaa ttaactattt attaattagt
      181 ttgtagatca agacattgcc agcgagacga aaaatcagac tttgctcttt ttggtgctgt
      241 caagttagag gacagtcctc ttagccccct cctttccccg ctcattcatt aaacaaatcc
      301 atcgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa
      361 agacatcagt tgaataaaca ttcacagaga tttttatgac acgcgttcaa tttaaacacc
      421 accatcatcc tgactagtct ttcaggcgat gtgtgctgga agacattcag atcttccagt
      481 ggtgcatgaa cgcatgagaa agcccccgga agatcacctt ccgggggctt ttttattgcg
      541 cggttgttaa cggttcagac aggtttaaag aggaataaga aaatgacaga caacactcgt
      601 ttacgcatag ctatgcagaa atccggccgt ttaagtgatg actcacgcga attactggcg
      661 cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga tcgcgatggc agaaaacatg
      721 ccgattgata ttctgcgcgt gcgtgacgat gacattccct gtctggtaat ggacggcgtg
      781 gtagaccttg ggattatcgg cgaaaacgtg ctggaagaag agctgcttaa ccgccgcgcc
      841 cagggtgaag atccgcgcta ctttaccctg cgtcgtctgg atttcggcgg ctgccgcctt
      901 tcgctggcaa cgccggttga tgaagcctgg gacggtccgc tctccttaaa cggtaaacgt
      961 atcgccacct cttatcctca cctgctcaag cgttatctcg accagaaagg tatctctttt
     1021 aaatcctgct tactgaacgg ttctgtcgaa gttgcgccgc gtgccgggct ggcggatgcg
     1081 atttgcgatc tggtttccac cggtgccacg ctggaagcta acggcctgcg cgaagtcgaa
     1141 gttatctacc gctcgaaagc ctgcctgatc cagcgcgatg gcgaaatgga agaatctaaa
     1201 cagcaactga tcgacaagct actgacccgt attcagggtg tgatccaggc gcgcgaatca
     1261 aaatacatca tgatgcacgc accgaccgaa cgcctggatg aagtcatcgc cctgctgcca
     1321 ggtgccgaac gcccaaccat tctgccgctg gcgggtgatc aacagcgcgt agcgatgcac
     1381 atggtgagta gcgaaaccct gttctgggaa acgatggaaa aactgaaagc actgggtgcc
     1441 agttcaatcc tggtcctgcc gattgagaag atgatggagt gatcgccatg agctttaaca
     1501 caatcattga ctggaatagc tgtactgcgg agcaacaacg ccagctgtta atgcgcccgg
     1561 cgatctccgc ttctgaaagc attacccgca ccgttaacga tattctcgat aacgtgaaaa
     1621 cgcgtggcga tgaggccctg cgtgaataca gcgcgaagtt tgataaaacc acggttaccg
     1681 cgctgaaggt gtctgctgat gagatcgccg ccgccagcga acgcctgagc gacgagctaa
     1741 aacaggcgat ggcggtggca gtaaagagta ttgaaacctt ccacactgcg caaaaactgc
     1801 cgccggtaga tgtagaaacg cagctaggcg tgcgttgcca gcaggtcacg cgcccggtgg
     1861 cttcagtggg tttgtatatt ccgggcggct ccgccccgct cttctcaacg gtattaatgc
     1921 tggcaactcc ggcgcgtatt gcgggctgta aaaaagtggt gctgtgctca ccgccgccga
     1981 ttgccgatga aattctttat gcggcgcagc tgtgcgatgt gcaggacgtg tttaacgtcg
     2041 gcggcgcaca ggccattgcc gcgctggcgt ttggtacgga atctgtgccg aaagtggaca
     2101 aaatcttcgg gccgggtaac gcctttgtca ccgaagcaaa acgccaggta agccagcgtc
     2161 tggacggtgc ggcgatcgat atgcccgcag gcccgtcgga agtgctggtg attgctgaca
     2221 gcggcgctac gccggatttt gtggcttctg atttgctttc tcaggctgaa cacggccctg
     2281 actcacaggt aattttactg acccccgatg ctgatatggc gcgtcgcgtt gccgaggccg
     2341 tcgaacgcca actggcagaa ctgccgcgtg ccgaaaccac ccgccaggca ctgaacgcca
     2401 gccgcctgat cgtgactaaa gatttagcgc agtgcgtgga gatctccaac cagtacggcc
     2461 cggagcacct gatcattcag acccgcaacg cccgcgaact ggtcgatggc atcaccagcg
     2521 ccggttcggt atttcttggt gactggtcac cggaatcggc aggcgactac gcctccggca
     2581 ccaaccacgt gctgccgact tacggttaca ccgccacctg ttccagcctc gggctggcag
     2641 atttccagaa acgcatgacc gtacaggaac tgtcgaaaga aggtttctcc gcgctggctt
     2701 cgaccattga aacactggcc gccgccgagc gcctgaccgc ccacaaaaat gccgttactt
     2761 tgcgtgttaa cgtccttaag gagcaagcat gagcaccgtg actattactg atttagcgcg
     2821 tgaaaacgtc cgcaacctga caccgtatca gtcggcgcgt cgtctgggcg gtaacggcga
     2881 cgtctggctg aacgccaacg aatatcccac agccgtggag tttaagctta ctcagcaaac
     2941 gctcaaccgc tacccggaat gccagccaaa agcggtgatt gaaaattacg cgcaatatgc
     3001 aggcgtaaaa ccggaacagg tgctggtcag ccgtggcgcg gacgaaggta ttgaactgct
     3061 gatccgcgct ttttgcgaac cgggtaaaga cgccatcctc tactgcccgc caacgtacgg
     3121 catgtacagc gtcagcgctg aaaccattgg tgtcgaatgc cgcacagtgc cgacgctgga
     3181 aaactggcaa ctggacttac agggcatttc cgacaagctg gacggcgtaa aagtggtcta
     3241 tgtttgcagc cccaacaacc ccaccgggca actgatcaat ccgcaggatt ttcgcaccct
     3301 gctggagcta acccgcggta aggcgattgt ggttgccgat gaagcttata tcgagttttg
     3361 cccgcaggca tcgttagctg gctggctggc ggaatatccg cacctggcga ttttgcgtac
     3421 cctttcgaaa gccttcgcgt tggcggggct tcgttgcgga tttacgctgg caaacgaaga
     3481 agtcatcaac ctgctgatga aagtgatccc cccctacccg ctctcgacgc cggttgccga
     3541 cattgccgca caggcgttaa gcccgcaggg aatcgtcgcc atgagcgaac gggtggcgca
     3601 aattattaca gaacgcgaat acctgattgc cgcactgaaa gagatcccct gcgtggagca
     3661 ggttttcgac tctgaaacca actacattct ggcgcgcttt aaagcctcca gtgcagtgtt
     3721 taaatctttg tgggatcagg gcattatctt acgtgatcag aataaacaac cctctttaag
     3781 cggctgcctg cgaattaccg tcggaacccg tgaagaaagc cagcgcgtca ttgacgcctt
     3841 acgtgcggag caagtttgat gagtcagaag tatcttttta tcgatcgcga tggaaccctg
     3901 attagcgaac cgccgagtga ttttcaggtg gaccgttttg acaaactcgc ctttgaaccg
     3961 ggcgtgatcc cggaattgct gaagctgcaa aaagcgggct acaagctggt gatgatcact
     4021 aatcaggatg gtctgggaac acaaagtttc ccgcaagcgg attttgatgg cccgcacaac
     4081 ctgatgatgc agatcttcac ctcgcaaggc gtgcagtttg atgaagtgct gatttgtccg
     4141 cacctgcccg ccgatgagtg cgactgccgt aagccgaaag taaaactggt ggagggttat
     4201 ctggctgagc aagcgatgga tcgtgccaac agttatgtaa ttggcgatcg cgcgaccgac
     4261 attcaactgg cggaaaacat gggcattaat ggtttacgct acgaccgcga aaccctgaac
     4321 tggccaatga ttggcgagca actcactaaa cgagaccgtt acgcccatgt agtgcgcaac
     4381 accaaagaga cgcagattga cgtccaggtg tggctggatc gcgaaggtgg cagcaagatt
     4441 aacaccggcg ttggcttctt tgatcacatg ctggatcaga tcgccaccca cggcggtttc
     4501 cgcatggaaa tcaatgtcaa aggcgacctc tatatcgacg atcaccacac cgtcgaagat
     4561 accggcctgg cgctgggcga agcgttaaaa attgccctcg gcgacaaacg cggtatttgc
     4621 cgctttggtt ttgtgctgcc gatggacgaa tgccttgccc gctgcgcact ggatatctct
     4681 ggtcgcccgc acctggaata taaagccgag tttacctacc agcgtgtggg cgatctcagc
     4741 actgaaatga tcgaacactt cttccgttcg ctctcttaca ccatgggcgt gacactacac
     4801 ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaaa gcctgttcaa agcctttggt
     4861 cgtaccctgc gccaggccat ccgcgtcgaa ggcgacaccc tgccctcgtc gaaaggagtg
     4921 ctgtaatgaa cgtggtgatc cttgatactg gctgcgccaa cctgaactct gtgaagtctg
     4981 ccattgcgcg ccacggttat gaacccaaag tcagccgtga cccggacatt gtgttgctgg
     5041 ccgataaact gtttttaccc ggcgtcggca ccgcacaagc agcgatggat caggtgcgtg
     5101 agcgcgagct gtttgatctc atcaaagcct gtacccaacc ggtgctgggc atctgcttag
     5161 ggatgcaact cctgggacga cgcagcgaag agagcaatgg cgtcgacttg ctgggcatca
     5221 tcgacgaaga cgtgccgaaa atgaccgact ttggtctgcc actgccacat atgggctgga
     5281 accgcgttta tccgcaggca ggcaaccgcc tgtttcaggg gattgaagac ggcgcgtact
     5341 tttactttgt tcacagctac gcaatgccag tcaatccgtg gaccatcgcc cagtgtaatt
     5401 acggcgaacc gttcaccgcg gcggtacaaa aagataactt ctacggcgtg cagttccacc
     5461 cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa cttcctggag atgtgatgat
     5521 tattccggca ttagatttaa tcgacggcac cgtggtgcgt ctccatcagg gcgattacgg
     5581 caaacaacgc gattacggta acgacccgct gccgcgctta caggattacg ctgcgcaagg
     5641 tgccgaagtg ttgcacctgg tggatctgac cggggcaaaa gatccggcta agcgtcaaat
     5701 cccgctgatt aaaaccctgg tcgcgggcgt taacgtcccg gtgcaggttg gcggcggtgt
     5761 tcgtagtgaa gaagacgtgg cggcgttact ggaagctggc gttgcgcgcg tggtggtcgg
     5821 ttccaccgca gtgaaatcac cggacgtagt gaaaggctgg tttgaacgtt tcggtgccga
     5881 tgcgctggtg ctggcactgg atgtccgtat tgacgagcaa ggcaacaagc aagtggcagt
     5941 cagcggctgg caagagaact cgggcgtttc actggaacaa ctggtggaaa cctatctgcc
     6001 cgtcggcctg aaacatgtgt tgtgtaccga tatctcgcgc gacggcacgc tggcgggatc
     6061 taacgtctct ttatatgaag aagtgtgtgc cagatatccg caagtggcgt ttcagtcctc
     6121 cggcggcatt ggcgacatta acgatgttgc tgccctacgt ggcactggcg tgcgcggcgt
     6181 aatagttggt cgtgcattac tggaaggtaa attcaccgtg aaggaggcca tcgcatgctg
     6241 gcaaaacgca taatcccgtg tctcgacgtt cgtgatggtc aggtggtgaa aggcgtacag
     6301 tttcgcaacc atgaaatcat tggcgatatc gtgccactgg caaaacgcta cgccgaagaa
     6361 ggtgcagatg aactggtgtt ctacgatatc accgcttcca gcgatggccg tgtggtagat
     6421 aaaagctggg tatctcgcgt ggcggaagtg atcgacattc cgttttgtgt ggcgggtggg
     6481 attaagtctc tggaagatgc cgcgaaaatt ctttcctttg gcgcggataa aatttccatc
     6541 aactcccctg cgctggcaga cccaacatta attactcgcc tggccgatcg ctttggcgtg
     6601 cagtgtattg tggttggtat tgatacctgg tacgacgccg aaaccggtaa atatcatgta
     6661 aatcaatata ccggcgatga aagccgcacc cgcgtcactc aatgggaaac gctcgactgg
     6721 gtagaggagg tgcaaaaacg cggcgccgga gaaatcgtcc tcaacatgat gaatcaggac
     6781 ggcgtgtgta acggttacga cctcaagcag ctgaaaaaag tgcgtgaagt ttgccacgtc
     6841 ccgctgattg cctccggtgg cgcgggcacc atggaacact tcctggaagc cttccgcgat
     6901 gccgacgttg acggtgcgct ggcagcttcc gtattccaca aacaaataat caatattggc
     6961 gaattaaaag cgtacctggc aacacagggc gtggagatca ggatatgtta acagaacaac
     7021 aacgtcgcga actggactgg gaaaaaaccg acggtctgat gccggtgatt gtgcaacacg
     7081 cggtatctgg cgaagtgtta atgctgggct acatgaaccc ggaagcctta gacaaaacca
     7141 tcgaaagcgg taaagtcacc ttcttctcgc gcactaaaca gcgactgtgg atcaaaggcg
     7201 aaacctcggg caattttctc aatgtggtaa gtattgcccc ggactgcgac aacgacacat
     7261 tactggtgct ggcgaatccc atcgggccaa cctgccacaa aggcaccagt agctgcttcg
     7321 gcaacaccgc tcaccagtgg ctgttcctgt atcaactgga acaactgctc gccgaacgta
     7381 aatatgccga cccggaaacc tcctacaccg ccaaactgta tgccagcggc actaaacgca
     7441 ttgcgcagaa agtgggcgaa gaaggcgtgg aaaccgcact ggcagcaacg gtacatgatc
     7501 gctttgagct gaccaacgag gcatctgact tgatgtatca cctgctggtg ctgttgcagg
     7561 atcaggatct ggatttaacg acagtaattg agaacctgca taaacggcat cagtgagtta
     7621 cgggctgagc aggtgtgatg ttgttgtcac acccggagcc attgcccgat gcgcaaactt
     7681 atcgtgccta cacattatct caacacacgt aggccggata agatgcgcca gcatcgcatc
     7741 cggcaaaaaa aacgggcatg gtgtcaccac cctgcccgtt tctcttaaat gcacaataat
     7801 attacttcgc gttgtaatta cgcagagcgt tacgccccaa cacaatcccc gcgccaacca
     7861 tgccgcccag aagcactgcc agaatcaagg tgattgcctt tttcggacta tcgcgacgaa
     7921 taggtaacgt cggtttcatc acatagcggt aagcatgaat atcaagatca tcaaacttta
     7981 atttttcaat atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg
     8041 gacgagtcgc ttcatgctta atcatcgact ccagcgcttc gctccctaga aggaacaacg
     8101 tatcttgcgt cacatcttca gtctgctgaa cctgtggctt tgtcacctgc gcctgattcg
     8161 catactgcaa cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacca
     8221 cttcctgggt tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtccttga
     8281 gatccttttc tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact
     8341 tcatttgtgc gccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt
     8401 tcttaacaga aggttcgatg gtaagttttt ctggctcttc ctgattatcc agcgtttctg
     8461 ctaatgcaga gaaggcagaa ctgaagcgac caattaacgt ctcctgcaaa tccgatactt
     8521 tcggtgcagc ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca
     8581 cgtcgggctg agtgataatt gctgttgacg tccatttctc cttcgctact gccaaataac
     8641 caatagccag ggcaatagcc acaatgacgg aaatgataat tgtcatcttg ccacgccaca
     8701 actgcactag taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat
     8761 tattattttc tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt
     8821 agcgataatc cccgtgaatg ctataggata agtgataaaa ataatgaatt attgcggagg
     8881 taacaaaaga caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag
     8941 agatcgcggg tgtagacctt atctgccaca tccttaagct cttctgccat acggttggag
     9001 ataatgacgt cggcttgttg tttgaaggtg gcgagatcac gttccaggcg agagttgaag
     9061 aatgagtctt ctttcatcac cggctcgtag atgatcactt caacgccttt cgccttgata
     9121 cgcttcataa tcccctgaat agaagacgca cggaagttat ctgacccgct cttcataatc
     9181 agacgataaa tacccaccac ttgcggtttg catcgacaat tgccgagatc aggttattcg
     9241 gcacagactg gtagttcgcc agtaactgct tggtatcttt cggcagacaa taaccaccat
     9301 aaccaaacga cggattgttg taatggttgc caatacgcgg atcgagacaa acgccttcga
     9361 taatctggcg agtattcaga cctaaacttt ctgcataact atccagttca ttaaagtatg
     9421 ctacgcgcat cgccagatag gtattagcga aaagtttaat cgcttctgct tcagtggagt
     9481 cggtaaacag ggtcgggata ttttgcttaa tcgcgccttc ctgtaacaac gcagcaaaac
     9541 gttctgcgcg ttctgaacgc tcaccgatga caatacgtga cggatggaga ttatcgtaaa
     9601 gggctttacc ttcgcggagg aattccggag agaaaataat attttcagta cgatatttct
     9661 tatgcatcgc tgcggtaaaa ccaacgggaa ccgttgattt gatgaccata accgcataag
     9721 gatttatctc aactacgtct ttaattactg attctacact ggatgtattg aaataattag
     9781 ttttaggatc atagtcggtt ggagtggcga tgatgacata atcagcatcc cggtaggctt
     9841 catttttatc taatgtggca ttaaagtgta ttttatctga ttgcaaaaac tgctgaattt
     9901 ccttatcaac aataggagat atccgatcat tcagcatagc aacgcgtgac ggtaaaatat
     9961 ctaatgccac aacctcatga ttttgtgcga ttagaagccc gtttgacaag cctacatagc
    10021 cagtaccgga aatggtgatt ttcatttcat gctctcagaa ttaacttaac tgtgaatcat
    10081 gatgttttta gcatcgtgat aagatgctaa aagttttaac gctacgttgt cagatggtca
    10141 acgcaacgaa aaacaattca gattttcttt ataagaatag ctgatgttta ttaagttaat
    10201 attaagcagt gaaatttagt ctataagata tttggcaaaa aaaagcccgg tgcaatatac
    10261 gccgggcctc aattttattg ttggttaaat cagattaatc cagccattca gtatggaaca
    10321 caccttcttt atcaatgcgc ttataagtat gcgccccgaa atagtcacgc tgtgcctgga
    10381 tcaggttcgc aggcagaacc gcggcacggt agctatcgta ataggcaacc gcagcggcga
    10441 aggtcggaac cgggataccg ttctgtactg cataagcgac gacatcgcgc agcgcctgct
    10501 ggtagtcatc ggcaatttgc ttgaagtacg gggccagcag caggttagcg atctgcggat
    10561 tttcggcata agcatcggtg attttctgca ggaactgcgc acggatgatg cagccagcac
    10621 ggaaaatctt cgcgatttcg ccgtagttca gatcccaatt gtattcttca gacgcagcac
    10681 gcagctgaga gaagccctga gcgtaagaaa cgattttgcc cagatacagc gcactgcgaa
    10741 ctttttcgat gaactcagcc ttgtcgcctg ctgactgagc ttgcggacca gagagaactt
    10801 tagatgcggc aacgcgctga tctttcagag aagagatata acgtgcaaac acagactcgg
    10861 taatcagcga cagcggttcg ccgagatcca gcgcgctctg gctggtccat ttaccggtgc
    10921 ctttgtttgc tgcttcatcc agaatcacat caaccaggta gttaccgtct tcatcttttt
    10981 tggtgaagat atctttagtg atgtcgatca ggtagctgct aagttcaccg ttattccact
    11041 cggtgaaagt ctgggccagt tcttcattag atagattcag gccacctttg agcagagaat
    11101 acgcttcagc aatgagctgc atatctccgt attcaatacc attgtgaacc atcttcacat
    11161 aatggcctgc gccgtcggca ccaatataag taacgcatgg ctcgccgtct tctgccacgg
    11221 cagcgatttt agtcaggata ggcgcaacca gctcataagc ctctttctgc ccgccaggca
    11281 taatagaagg acctttcagc gcgccctctt caccgccgga aacaccggta ccgatgaagt
    11341 taaagccctc tgctgaaagc tcacgattac gacgaatggt gtcctggaag aaggtattac
    11401 caccatcaat gatgatgtca cctttatcga gatatggttt cagggaatca atcgcagcat
    11461 ccgtgcctgc acctgctttc accattaaca ggatgcgacg aggcgtttcc agagattcaa
    11521 caaactcttt caccgtatag taaggagcca gtttcttgcc tggattttca gcaatcactt
    11581 cttccgtctt ttcgcgggag cggttgaaaa tagagacggt ataaccacga ctttcgatgt
    11641 tgagcgcaag gttgcgcccc atcactgcca taccgactac gccgatctgt tgctttgaca
    11701 ttgtttactc ctgtcttcta tattattaac cgtctattaa ccgttatatt attaaccgtc
    11761 gtgattttca tcttctaatc aaggaataca tatggttaca gcatgcttct tactttatag
    11821 cgataagtat gccattgggt gtatccataa gaactttgaa ttttggatac ttttctggat
    11881 taaaattagc atagcttcgt ttagatataa caataaacga actattaaaa gatttcatct
    11941 tctctattaa atcatcctgt gggattacct cacactcggg attactaata gtccaatcac
    12001 tatatcttgc taatccaaag acgtcaccaa ttgccatccc tttatagaag taaataccat
    12061 cctccaagcc taattggata agattatctc catataactc actatattca cttgctttct
    12121 tcatcagttc atagctatag cgactttcta agcgtgaatt aaaattcaag tattcagaat
    12181 aaaaatattt gaattcaaaa tacaacgatc gccccgaaaa aaaaataata cacacaagga
    12241 taaaatatcg tcccaacttg taatttgtaa tgttgatatc ttttattaaa cttgcaattg
    12301 agatcgcaac ctgcaatact gacaatgggt aaatgggtga taaatacctg tcaccttgcg
    12361 taacagaaaa ccaaaataag acatatgtta aaacaatcga gaaaaatacc caaagtattt
    12421 tattattcct aagtgtaaag acagacagta tagcataaaa aacaacatca ctgccaactt
    12481 tattaattgc ttgttgcaaa tctaatgtgg caggacttat accatgtcga gcttgttctg
    12541 ctgtttgaac ttgtagatca atttcattcc ataggtagtg accaaatata tttcctccag
    12601 caggactaaa aggatcacca gagatccaaa aactacggac ataccaataa atgccaccaa
    12661 gaattgttgc caagctaaaa ataagaaatg tctgtatgtt tctctttttc gctatcaata
    12721 taacaaatat gatggctcca tatatggcac caaatagttt tacgccacaa gcaattccaa
    12781 taaaaaaacc acataaaaaa attaattcat tttgtgtttt attgttttct gagcgaatat
    12841 atataagaat actggctgca gcaaaacaaa gatagcgtta aatcaatata tgcataacca
    12901 agatgatctt taagtggatt gatagtaaat agtaggattg tagcaataat gcctggataa
    12961 taagttttcg taatttccca tgaacagcca acaagtccac aggctattac aaaaaaacag
    13021 atatttgcta aaaactgagc taaccttaca tctcctaact gtaaacctaa agccattaaa
    13081 agatctgcat tttgcgggaa cagaggaaaa cgcaaatatt gttcaacaac aattgcatgg
    13141 tgctcaacta atgaacgagc caaaggtaag tgatacatag tatcatccca atcaccggga
    13201 gctcgcaggg acgataacaa tgtaaccaca aaaatcaaaa caagaaataa aataacaaac
    13261 agcgtactga actcaatatt atttattgcc agccatctaa attgctgacg tttaataatg
    13321 cttaaaaccc aacacaccgt aaaaattaaa aagatagcaa tcttattaaa ttgcccgacc
    13381 atgcccagaa agtaaattat caaaatattt aaagacatcc ctatgcatag gcaataaaaa
    13441 ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac aatacaatat
    13501 taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac aaatacaacg
    13561 gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt ttttcagaaa
    13621 gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa ataatgccac
    13681 atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca gtcggaaatc
    13741 ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa attatagaca
    13801 aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca agcgagtaaa
    13861 gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt ttgctcacac
    13921 tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata cgtaattcta
    13981 aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat ggaaaagatt
    14041 taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa ttccctttaa
    14101 agatagtctg aacagtacca gtcaacaatt tatttcctca gcgatgccct tttctgtatg
    14161 tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga ttttttatga
    14221 gatggttaat catttctgga gcagaacttg catcataagt gtcatctcca tcaaccatta
    14281 gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc ttgcctttca
    14341 gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta ctgtcagtcg
    14401 aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa tcatttatga
    14461 cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata ttaaaagagt
    14521 gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt gtttttaaca
    14581 aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa ccaaactaaa
    14641 aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa ataaaacgcc
    14701 gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag ccttggttgc
    14761 agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg cacatataaa
    14821 accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg cataaaatac
    14881 cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa aagaactggt
    14941 caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt tattactaat
    15001 ttggatattt tctatagaaa atacccataa aactacaacg cctataataa aaattcccat
    15061 gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa gtattaacga
    15121 catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga taagcaacat
    15181 aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct caagaggtac
    15241 tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa acaagaacgt
    15301 cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag agtcagaatc
    15361 ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc ccaatattat
    15421 tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca gtgataatgt
    15481 tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta ttatgagaca
    15541 ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa agtagaagga
    15601 tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg ataagtgttc
    15661 ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg aaatatataa
    15721 cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac atgaaaatac
    15781 ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa ggtatggttg
    15841 cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa agaagccata
    15901 aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga ttgaaaaatt
    15961 gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata aatatcgcaa
    16021 agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca gttcaataca
    16081 taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat gttttttaaa
    16141 atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc cgccttaact
    16201 tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat tgattattca
    16261 caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga taccacttgt
    16321 ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct cccacttggt
    16381 tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac gaataatttc
    16441 tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt gcagcatttt
    16501 tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata acatatgtac
    16561 accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga gccttatcaa
    16621 ctaaattttt tcgccctgat ggataatatg caattacatt gctggaataa caattcgcat
    16681 tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa atatcgtcct
    16741 gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt aatctataaa
    16801 aatttttacc tgccccccca aacttattga cgcttggaag tatatttata attgagggat
    16861 atgataattg aaattcttca caaatattta atgtattatc agttgataaa tcatctgata
    16921 taaaaattga gatgtctaca tctttttgtt caataatact ttttaattgt tcctcaatcc
    16981 agcattcgcc attatacgta gccataagta cagctactct tttttgatca gaactatgca
    17041 ttataacgac cgcccccagt aattcctctt attccatcca gcgtagccaa aatatgtttt
    17101 ttcatatatt caattttatt tttttgagtt gcgataatta aagtcgtatg aattaaagaa
    17161 aaaacaactt ccctaataga gagtaaaaga ggcacatgat tttttctaag caaaagaaat
    17221 gcatttctta ctcgataata acgacgtact ggcgagtgaa ttggtatgtt aatcccacag
    17281 atttttactg acttatcccc aatagcatga accatttcta tatcaggaat gatatgaact
    17341 aatatgccat agtttaagca tctaagacac cattctgtat cgacataatc aataaaaagt
    17401 tcctcatcca tcattccaac aatctcaaaa acttcttttg aaaccatggt tccggatgag
    17461 attgttactg aacttttaaa cggtgtctgt ccttcagtga catgaatttt ctctcgaaga
    17521 ccattttttt tgatattaca aataggataa taatgtgact tatctctatc aataaaaact
    17581 gggccagcta tttttgcatt ttcattatcc atatagcagg aaagtttttt gataaactca
    17641 ttgcgaatac ggctatcttg atcaaaaaaa attatcttct ctgccccttt ttcaagagaa
    17701 tatcgaatcc caatattttg tgcttttgct ataccttcat tcctaccaag acaaattaaa
    17761 tgaacttttt caatgtctgc taatttatct ttcaaggtga aattagtcct attatcgact
    17821 actacaacgt aacaattttg ctcctttagt tcagtgatca gtgcattcag atttttaagt
    17881 tcgggattat atgtcacaat gacagcgtaa atattactat tcattatcca agtgactcag
    17941 taattggtta attttagttt tcatatacag aacacattct agtataaagc cattgtaata
    18001 acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt aacccatatt
    18061 tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa tgaatcaaag
    18121 cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt gccagcgcat
    18181 tgaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt tgataattct
    18241 caccaaacca tattttcaat acaagccctg aatataacaa gcctatgatt atcaccggca
    18301 aacagattag aaacattaaa agcaatgatt tatttacatt acgtttaaat tctttaaagt
    18361 ctttgatgtt acttaacctt ggaaacactg ccctcccgat tgctgcaggt attattccta
    18421 attttagaat aacctccgct ggtgcagaat aaaatgcaac tttatctgcc cccataatat
    18481 ttgacactat aaatcgatca aaatacacca tgaccggact tatgatatta cttactgtca
    18541 tccaaccgcc aaagaaaaag agtcgtttaa aggttttata acaaaaatga acccccgaaa
    18601 ttttaatatc attcctgaca taataggcag agactaaaat cgaaataact cttgcaaata
    18661 ttaaaccagc aaccgctgcc gacaatgtag cactataaaa aacaaatatg gccggaattc
    18721 ccgcgatgca agagcttgat atagattttt gaatatttac aatgccaaat ttttcatccc
    18781 cctcaagaat ggctgaccat aattgattta gaataaataa tggaatgcaa atagccaaca
    18841 gtttaaatgc tagttgtata tcactatgtt caacaccaga aatttttaga taattaacaa
    18901 ttccatcaga gaaaatcaat aataaaaaag caccaaagca tgaaaatagc accaaaaaag
    18961 atgtacttgt tgaaattacc ttggttcttt catgatgatt atcacgatga attgcaattt
    19021 ctctaataac agagcgagtc aggcctacat caaaaattcc agcataccca acaagtgcaa
    19081 ttgcaattgt ataaacaccg aaaccttcag gccccaaacc tcgagcgaga aatcctagcg
    19141 aggggatagc aattaaagtt ggtattgcat agccaaaaag gttccagaca ctatttttta
    19201 ttatgctcat tattacattt ctttatcata taaaataatc agcatctaca aatttttgac
    19261 cattaatatc tttatcagaa agcaatggat tttgaaccgg ccattctata tttatacttt
    19321 tgtcgctcca taaaatccca cgctcttgca tttttgaata gaaattattt gttttgtaga
    19381 caaattctgc ttcatcacta agaacgcaaa acccatgagc gaatccttcc ggtatccata
    19441 attgacgctt attctccgcc gacaaattta caccaaccca cttgccaaat gttggtgatg
    19501 atttccgaat atcaacagct acgtcaaaaa cttcaccgac aacacagcgt accagttttc
    19561 cttgagcata aggttccagc tgatagtgca gtccgcgtaa cacaccctta gttgatttag
    19621 aatggttatc ctgaacaaac tcaacctttc gtcctacagc ctcttcgaaa actttctgat
    19681 tgaagctttc cataaaaaag ccccgctcat caccaaatac ttttggttca aaaattagca
    19741 catcaggaat ttcagtttta attacgttca ttttattaat aacctttaat cattttcagc
    19801 agatactgac cataagcatt tttcttcagc ggctcggcta ataccttcac ttgctcagca
    19861 tcaataaagc ctttacggtg agcaatttct tccgggcagg aaactttcag cccctgacgc
    19921 tcttcaattg ttgcaatgaa gttgcttgct tcaataagac tttgatgtgt ccccgtgtcc
    19981 agccatgcat aaccacgccc catcatagcg acagacaaac gtccttggtc catataaata
    20041 cggttaatat cggtaatttc cagttcgcca cgtgcagatg gtttaaggtt tttcgccatt
    20101 tctacaacgt cattgtcata gaaataaagc ccagtaaccg catagttact ttttggttcc
    20161 tgcggttttt cttccagact aattgccgta ccgttatcat caaattccac cacaccgtag
    20221 cgctcaggat catttacgtg ataggcaaaa accgttgcgc cactctctct gttaacagcg
    20281 gtatccatta atttcggcag gtcgtgacca tagaagatat tatcaccaag taccagcgca
    20341 caatcatcac cacctataaa ttcttcacca atgataaatg cttgcgccag accatccgga
    20401 ctcggttgta ctttatattg cagattaagc ccccactggc ttccatcacc aagcaattgt
    20461 tgaaaacgtg gtgtatcctg cggcgtactg ataatcagaa tatctcgaat acccgccaac
    20521 ataagcgttg aaagcggata ataaatcatc ggtttatcat aaatcggtag cagttgtttg
    20581 ctcacagcca ttgtcacagg ataaagacga gtgccggaac caccagccag aataatacct
    20641 ttacgcgttt tcatttcacc attcctttta attcatctcg ctctagcatc atgagcgaga
    20701 tgcaataatt tgttaaattg ccgtggtcgt aaataattcg ttgagcatac gtttcacgcc
    20761 cacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttcgg tattgaggcg
    20821 agaattatgg ggacgacgtg ctggtgtggg ataggccgtt gtcggcacgg cattaagttt
    20881 gttaagagca aggttgatcc ccgctctgcg cgcttcttca aataccagag cggcataatc
    20941 gtgccaggtt gttgtaccac cagcaaccag atggtacaaa ccagcaactt ctggtttatt
    21001 tgcggccacg cgaatggcat gagcggtgca atcagccagt aattcagccc ccgttggtgc
    21061 gccgaattga tcgtttatta cagccaactc ttcgcgttct tttgccaggc gcaacatcgt
    21121 tttggcaaag ttatttcctt taccagcgta tacccagctg gtacggaaaa taagatgctt
    21181 tgcgcaatgt tcttgtaacg ctttttcccc agctagcttg gtttcaccgt agacatttag
    21241 cggtgctgtt gcatccgttt ccagccatgg cgtgtcaccg ttgcctggga aaacataatc
    21301 agtggagtag tgtataaccc aggctccgac ttcattagct gcttttgcaa tcgcttcgac
    21361 gcatgtcgcg ttaagtaatt gcgcgaaatt cggttctgat tccgctttgt ctactgcagt
    21421 gtgagcagca gcattaacaa taacatcggg gcgaattttt ttgacggttt cagccacacc
    21481 ttcggggttg ctgaaatctc cacaataatc agtggagtga acatcgagcg caatcaagtt
    21541 acccagagga gccagagcac gctgcagttc ccaacccacc tgccctgttt tgccgaaaag
    21601 caggatattc attactgacg gccctcatag ttttgttcaa tccacgactg ataagtaccg
    21661 cttttcacat tctcaaccca atttgtatta gccagatacc attccaccgt tttacgaatc
    21721 ccgctctcaa acgtttcttg cggtttccag cccaattcgc ggctaatttt atcggcatca
    21781 atggcgtaac ggcggtcatg ccccgggcga tcggcaacat aagtaatttg ctcacgataa
    21841 gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac tacgtcgatg
    21901 tttttctttt cgttatgtcc accaatgtta taagtttcac ccgctttacc ttcggttacg
    21961 acggtatata acgcacgagc atgatcctct acatacaacc agtcgcgaat ttgatcccct
    22021 ttaccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa tggaataagt
    22081 ttttccggga agtgataagg accataattg ttcgagcaat tagtcacaat ggttggtaaa
    22141 ccataagtcc gcttccaggc acgtaccaaa tgatcgctgg aagctttaga cgcagaatac
    22201 gggctacttg gcgcgtaagc tgtcgtttcc gtaaatagcg gtaacgcttc gttattattt
    22261 gcttcatccg gatggggtaa gtcaccataa acttcatcag tagaaatatg atgaaagcgg
    22321 aaactttttt tcttttcatc attcagagca gaccagtaat tgcgcgccgc ttctaaaaga
    22381 acataagtcc ccacaatatt ggtttcaata aatgccgcag ggcccgttat tgaacggtca
    22441 acgtggctct ctgccgccag gtgcatcacc gcgtctggct ggtgctgtgc gaaaatacgc
    22501 gacatggcta cagcatcgca gatatctgca tgctcaaaag cataacgttc agaatcagaa
    22561 acatcagcga gtgattccag gttcccggcg tatgttaatt tatcgacatt aacaacacta
    22621 tcttgcgtat tatttattat gtgacgaaca acagcagaac caataaaccc tgcgccacca
    22681 gtaacaagta tcttcacttt tttattccat atagctatag agcatgctgt gaaatagact
    22741 gctccagatt tgattaatag aggtattaat acacgctacc gcccctggct taacagctac
    22801 cagtgcactg catgcatctc aacgatgtgg cgagcgttag ccactcgcgc caaatccgaa
    22861 aaattcaaac gttaattgtc ttaccaaaca gccgtagaaa caaggaaaat cctggaaaaa
    22921 tttgaataat atgagctatg taacttcttg tttttatagg ctttataaaa acggcaacgg
    22981 caggaaaatt caatgagcaa aacttatgcc acgaatcttc actgccgtta taattttctt
    23041 atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc aataccttta
    23101 cggaacttcg ccccttcttt caggttgcgt aggccatact tcacaaacgc ctgcatatag
    23161 cccatttttt tgccgcagtc gtaactgtcg ccggtcatca gcattgcatc aacggattgt
    23221 tttttcgcca gctcggcaat agcatcagtc agctgaatac gtccccatgc accaggctga
    23281 gtacgttcca gttccggcca aatatcggca gaaagcacat agcgacctac ggccatgatg
    23341 tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat gcggctgact
    23401 ttaccctcac ggtccagcgg ctctttagtc tggatgacgg agtattcaga gaggtcaccc
    23461 ggcatacgtt ttgccagcac ctggctgcgg cccgtttcgt tgaaacgtgc aatcatggca
    23521 gcaaggttgt aacgtagcgg gtcggcgctg gcatcgtcga tcacaacgtc tggcagtacc
    23581 acgacaaatg ggttgtcacc aatggcaggt cgcgcacaca aaatggagtg gcctaaacct
    23641 aaaggttcgc cctgacgcac gttcataatg atcacgcccg gcggacagat ggactgtact
    23701 tccgccagca gttgacgctt cacgcgctgc tcaaggagtg attctaactc ataagaggtg
    23761 tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat ttctttgatc
    23821 cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac gattggtagc
    23881 atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccgagacc cgctacagga
    23941 ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc tgaattatag
    24001 cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa ttacttgtga
    24061 tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc tccatttttc
    24121 caggaatggt cgcaaatcta ctccctccgt tccggcaatc taaagttaat cttttccaca
    24181 ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct ttcattcacc
    24241 gcatgaacat ttgcaagaga cagcagtgtt tcttttttcg ccataaagac gccgcgaacg
    24301 tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc ctgcatcaca
    24361 ttgatatttc gcataaaaag atgttgcggc ttgttgtgta actccagcga ggcacgcttc
    24421 atctcaatgt tagttagcga cacaaaggag acggcattcc cggcggagat ttggatgccg
    24481 cgcaatttat aagcaagatg ggtattatcc agttgaatat tattcactcg gaaattttgc
    24541 ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc ggcgctatta
    24601 atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcgac tgtcgcgtta
    24661 tcaatgcccg ctttcttact gaaatccggc gtgatattgc gggcattgat attacgaata
    24721 acaaaatgtt taccattttc aacatggatc aactgccgac aatccgatcc cgtgatattc
    24781 gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcata agtgcttccc
    24841 gcaaggccta tgccgatgcc ccagttgatt ttgccgttgg tacagttgat acgctcgatg
    24901 acatggtcag atatcaaaat atcactgtcg ttaattgcca cgttccattc aatggcgtcg
    24961 ccctgtaagt cgctgaactt acagttggta atgttggcac cgataatctg gttatgaaat
    25021 ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat ggtcaggttg
    25081 cgcatgaccc gtttgttttt gccgccaata taaatctgca ttaccgggcc aaagccgctc
    25141 atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtaat gttatgcata
    25201 ccgccgccct ctccccctgt cacctggctg ccgtcctgta agacaaatcg ccctctgccg
    25261 ttgccgcgca ggcttccaag gatgtgtaac gttttgccgg gagggatgaa gatgccggtg
    25321 ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc gctgaaggct
    25381 tgtttaaatg aggcgatcca gtcatgtggg ttgtagtcgt taatgttaac gctttgtcgg
    25441 gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct tgccgtcagg
    25501 aaggtgcgtc gggagagtgt tttaaatggc atacgttctc ctctataaag cctgcagcaa
    25561 gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt tttcgcacgc
    25621 acgtttgacg accggagcca gttcgtcggt atccagttgg ctaaacgccg ccagacgttg
    25681 tgccagtgcg cgagcatcgt tctcaggcag cagccagccg gatttatcgg cctccaccag
    25741 ttcaggaatt ccgctatgca gagtagaaac caccggaata ccgaccgcca tcgcttccat
    25801 tagcgccacc ggaatgcctt ccatatcgcc atccgcgccc gttaccgatg gcaacaggaa
    25861 gacatccgcg tcgtcgagca tctccttcac ttcatggctc ggtttaaagc ccggcatctc
    25921 taccacatct tccagttgat attgttcgat gatggtgcgc aggcgtcgtt cccacaggcc
    25981 aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac ggcaggcttc
    26041 aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acagatatga tttccagcgg
    26101 cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc ccatacgcga
    26161 tacggcgatt ttttctctgg ggcagcccat tttttgcagc cttccggccc acagatcgct
    26221 tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag tgtagtggtt
    26281 gagcacttcc cgactggaga tatcaatacc gtggaagatg gtggcaattt tgccgcgaat
    26341 gatacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat gagcgatgaa
    26401 cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa tcaggttccg
    26461 cgactcggca ccatagcgtt tgaggttgag cgcatgccag gtatttttac gatgaatgcc
    26521 acgtaaggtc tggctggcgc ggtggcgcag tttcgctact ttgcccgcag gttcgtcctg
    26581 taaccagcgg gttctggcga caaggttgta tttcgtccat gccgcgtggg tgttctctgt
    26641 atcgcctttt tgcaaagcga caatctccac ttcaaagccc atatcaataa acgcggtaat
    26701 ttgattgagg acgaaggttt ccgacgacag cgggaatttc aataagaaga agccgacctt
    26761 catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc cgtctgacgt
    26821 tcgcgactga cggcttcgtt aaatcgagcg ttcagcaccg gaagctggcc taaggtatcc
    26881 gcaaccatcg cttgcaggct gccgtccagt aaatgacgaa tatcaatcgc catctctggc
    26941 agtcccagct gctgcataat cccggcggat ttatgttcat agttgatggc aatcgccgga
    27001 gtagcaaagt tcattgagat aatggcggag tgcaggcgcg taccgacggt gagttcacag
    27061 gctccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcactac gtggtaacgg
    27121 gcaggatcgc tgatgtgctg gcgcagattg agcgccacca tgcggtcgtc tttgttatag
    27181 ctgtcgatac ccgtacaggt ggagagcgca atcacctgat agccttcatc gagaatgcga
    27241 ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag acgtttgtcg
    27301 aacggtgcca gttcgcgcag ggtaattgcc actgttttct gttgtgcggc aacgtccagc
    27361 cagtgttgaa cggcatagct ggcggtgaag tcttcggtgt ggtgatcgac cagccacgcg
    27421 gtatcgacgc catgttccac ttttgcggtg gtgatatttc tgcgtttcat caaatcaagg
    27481 ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa agacatagtt cgccagttgg
    27541 ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag cggctttttc
    27601 gccataaatg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc gacaaaaaac
    27661 gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa gtcggtgaat
    27721 ccctgggcga tggcgatatt gcgcagcttg ccagtgtcgg tgacgcgtga gagcaatacc
    27781 tgatgctggt agcggcgacg gaggactttt ttaatgcgcc cgacaacgcc cgccgcgcta
    27841 ttgtgttgtg tcatctgcag gaacagcgga tcgcccatta ccgggcggtt gagcagccag
    27901 gaagaactga ccggatagcg gctcatcacg tccacttcgg tatgtggatt gaggatgttg
    27961 atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca agtgtggttg
    28021 cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag gctgtgtttg
    28081 ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca ggcctacagg
    28141 tcccgagcac agaaccgtag gacggataag gcgttttacg ccgcatccgg caaccgttgt
    28201 cggaaccgaa aacagcaatt caccccgccc gtaaaagcat tttcattttt tcgctgcgac
    28261 aaaactgacg cttcacttcc accaccagcg gatggcgtga cagcacaatc atcaccacaa
    28321 acgccagcac gcccgcggct atttgcaccg ccagcagcat ccccagcgcc agttgccctt
    28381 tcagcacaat gcccagcgca taactgacca ccagcgtcgg caacgagaga taaaacggca
    28441 gccacaaact caggatgtac tggcgataac tggaaccaag aaccggttta atcatcacga
    28501 agtaactcag aatggtgttg ataatctgca ccagcaggaa accgagcgtg acgccgattg
    28561 cgcccgccat ctgcccacca ataacaatcg ccggaataaa cagaaatgtt ttgaatacgt
    28621 tgaatttaaa gctgatatcg acccgcgctt tcgccatcag cagcgaacca atcgggttac
    28681 ccacggagcg cagcagaccc acaatacaca gcaattgcag caccggaata atgctgttcc
    28741 acttttcgcc gaataccagc ggtacgaagt tattcgacac caccatcagc cccagcagcg
    28801 ccggaaagtt gataatcccc actatcgaca gcagcttgta gaagttaaca cgcagctttt
    28861 cggtgtcgtc ctgaattttg gcaaacgccg gaaacaacac gcgggtgatg attgggttca
    28921 gcttcatcgg cggtacgacg gccacgttgt acgccaggtt gtatccccct gccacgcccg
    28981 cgccaagaat acgcgccagc accagcgttg aaaggttggt gttgagatag ttgatgatgc
    29041 tgtccgccgt cagccaggca ccaaaacgta agttcggtgc cacagacgcc agcgagaagt
    29101 gcagaccggg gcaataaatt ttgcgtccaa agtagccaaa cagcagtgtt ctcaccgcac
    29161 tattgaccag ataaccgagg atcgcggtca tcgccagcgg ccagaaatgg gcgctaacta
    29221 ccgtaaaagt gaagcccgcc agcaccgcgc tggtttcgat catgccgatt ttgttgaact
    29281 ccagctcctt ctgcatcaac gcgcggaact gttgcccgtg ggggatcacc acaaacgcca
    29341 gcgacaatgt tttaatcaac ggtgccaggt ccgggttatt cagcacgtcg ccgatgacat
    29401 cactcaacaa aaacaccgcc acacatacca caagccccag cccgacgttc agccagtaca
    29461 gcgtggtgag ttcaaggtga ctgatttctt ttcgctgaat aatcgagtta gcgataccga
    29521 agtcagaaag cgtatctgcc agcgcgataa tcaccagcga cacagtcagc aggccgaact
    29581 ggtggttgtc gataatccgc gccagcacgg tcatctgcac cagcccgagg ccgatgatga
    29641 tcaccgtggc aatagccgac cacttcgcgc cgctgatggt tttttcacgt aagctcatat
    29701 caatatgccg ctttgttaac gaagcccttg aataccgtca ggaaaacgat tttgatatcg
    29761 aaccagacgc tccattcgcg gatgtactca aggtcgaact cgacgcgttt ttccattttc
    29821 tccagcgtgt cggtttcgcc gcgccagcca ttaatctgcg cccagccggt aatgcccggt
    29881 ttcactttat ggcgcagcat gtagccttca atgagctggc gatactgttc gttatgcgct
    29941 actgcgtgcg gacgtggacc gacaatcgac atccccccgg tcagcacatt gataaactgc
    30001 ggcaattcgt ccagcgaggt gcggcgcaga aagttcccca ctttagtgac gcgcggatcg
    30061 ttctgcgtcg cctgggtcac cactttgtcg ttctccatca ctttcatgga acggaacttc
    30121 cacactttga tcggcttgcc atccatacca tagcgagtct ggcggaaaat aaccggccct
    30181 ggtgaactga gtttcaccgc cagcgcaata cagcacagca ccggggagat cagcagcaga
    30241 ataagcgtcg ccagcacaat gtcttccgca cgttttagca ggcggttaac ccccgaaagc
    30301 ggcgtgtcat acagcggcac caccggaacg ccgttcatct cttcgagacg tgaatggaga
    30361 atgttgaagg taaagacgtc ggggatcagc agcaccgaac aggtggtatc cgccagttga
    30421 tggaccagtt ttttcactcg tgcgccgtcg cacatttgca tcgcgatata gacgttatga
    30481 atcttgcccg cttttgcatc ctcgaccagc tgttgcaggt tacccgccca gtcgttagaa
    30541 acgccgcccg gtttcgggtc gtggtaaacg cccaccactt caaaccctaa ccacggctga
    30601 ttacggaaac tctccatcag catttgtccg gcggctaaat cccccgctac ggcgaccatc
    30661 cgcttgttgt agccatgatt acgcagccag cccgccccaa tgcgaataca tgaacggcaa
    30721 accaccagtc cgatgctggt cagcccatac cacgccagcc agattttcag ttgcgtgtcg
    30781 aaatcattgt tgaacgccac cagtccggcg ctgaaaatca cgcttaaggt ccagttttgt
    30841 agcaacaggg caaattctgt cgctgcccga acaccgcgcc atgagcgata aaaatcggtg
    30901 atgccgccca gcatctggaa caccaccagc gtaatcagcg ccaccaacag gtgcatgtag
    30961 aggaatgaca gtccgctgac ttcgcaaacc agccatagcc cggcaaacat gatggtgata
    31021 tctgaaaagc gttgcaccat agagattaac gatgcattgg ttttcgctcg ctcgcgcttt
    31081 tttagatttg tcatcgttgt tcctgttatt agccccttac ccgcagtggg gtaagggaag
    31141 atccgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc catcagcggc
    31201 acatcaccgc gcgattccac attcaggcgc accaccggtt cggtattgga ggtgcgcagg
    31261 ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgc tgcgatccac cgccagcgcc
    31321 tcacggctaa aatgctgttc cacgcggtta atcgcctcaa cgggttgcgc cagtttgctg
    31381 ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc gcccagcgtt
    31441 tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc gctgtcgcag
    31501 taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat ggcgtcttcc
    31561 ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acattaccgg cgtgccgcct
    31621 gcagcagtca ccacatcaat agtgttccaa gagagacgcg gatcatggat gatcttcgcg
    31681 ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta gccctcgata
    31741 aactgccctt tttcgtcaaa cagaaaacag cggtcaaaat cgccatcaaa ggcaatgccc
    31801 atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcgacattc cggcagcagc
    31861 gggttaggaa taccgttggg gaaattgccg tccggtgtgt tgtgtacttt gattaattcc
    31921 acgggcgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg acccgctgcg
    31981 ccattcccgg agttgatcac cagcttgagc ggcgtgagat ttttgacatt gatataaccg
    32041 aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc gcgtttggtt
    32101 tcatcgacgg gaggaaagtc gttggcttca gccagacgct ggacgtcgcg cagtccggta
    32161 tcaccgctga tcgtgcgagc cccctcgcgc accagcttca tgccgttgta atccatcgga
    32221 ttatggctgg cagtaacttc aatgccgcca tccacgccga gatggaacgt ggcgaaatag
    32281 atctcttcgg tgccggacat accgatatcc agcacatcga cgcccgcatc ctgtaaacct
    32341 tttgccagcg ccagttttaa ggtttcgctg gtgagacgga catcaccgcc taacacaatg
    32401 gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat atcttcattc
    32461 agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa ttttttcatg
    32521 tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccagttt tatttttgtc
    32581 gttatttaca ccgcggtttc gcattcattg cctgatgcga cgctgccgcg tcttatcagg
    32641 cctacaaaac cgaaccgtag gtcggataag gcgttcacgc cgcatccgac aaacagcgcc
    32701 tgatgcgacg tttacacccg tccgtagcga tccgcgaagc gcaccacatc atcctcttcg
    32761 agataagagc cggagcgcac ttcaattaaa tcgagcggaa ttttccccgg gttttccagg
    32821 cagtgcgtcg cccccagcgg aatataaatg gactcgtttt caccaagcag tttgatatca
    32881 ccatcaatgg tgacttttgc cgttcccgcg accactaccc agtgttccgc gcggtgatgg
    32941 tgcatctgta ccgacaaacc ctcgcccggt ttcacggtga tgcgtttcac ctggtagcgg
    33001 tcgcccgcgt cgatagagtc atatttgccc cacggacgat acacttcgcg atgcacccga
    33061 tgctcatggc gaccatcggc tttgatctgc tcgaccactt ttttcacatc ctgtaccgcg
    33121 ttacggtcgg caatcagcac cgcatctttg gtctgcacca ctaccagatc tttcaccccg
    33181 acggtggtga ccaggcctga ttcggcatac acatagctgt tttcagtttt gtgattaatc
    33241 acatcgccgt ggcaaacgtt gccctcggcg gtgtgggcgc tgatctccca taatgaagac
    33301 caggaaccaa catcgctcca gcccgcatcc atcggcacca caacagcatc tgccgtaggt
    33361 tccatgaccg cgtaatccac cgactcttcc gggcaggcga gaaacgcttc ttcatccaca
    33421 cgaataaaat cgagatccgg atcgacggcg ctcatcgctt tttcgcaggc atcgaggata
    33481 tccgggcgat attttttcag ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg
    33541 ctgttccagt aatattcgcc gcttgctaca taggcctggg cggtttccag attcggtttt
    33601 tcgacaaact gcgccacttc aaaggccacc gtatcctgct cacccacaga cacttcaccg
    33661 cgacgaatat agccataacc ggtttctggt agatccggca caatgccaaa ggtcaccagc
    33721 ttgcccgctt cggcataagg catggcatta cgcacggcgg cacggaacgc gtcttcatcg
    33781 gcaatcacat gatccgccgc caataccagc attaacgggt cgctctccgg gctatgacgt
    33841 tttgccgcca gcgccgccag tgcaatggca ggtgcagtgt tacgccctgc cggttcgaga
    33901 ataatgttct cggtgagttt gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc
    33961 tgctcattgc aaatcaccac cgggctttcg cactccacgc cgttcaggcg gcagatggtg
    34021 gtttgcagca tggtgagatc gcctttcagg cataaaaact gcttggggta aagtacgcgg
    34081 gaaagcggcc ataaacggct accggagcca cctgccatca caactggata gagtttcgac
    34141 tgcgccataa ttatccccga atatcattta taaattgacg taacacgttc tctttatcga
    34201 gcgtgcgttc ggcatattca cgtgccaccg tgttatgttt gggcagcagg agcgcctgac
    34261 gaatccccgc caccagcgcc tcgaccgatt ccggttctac gcaaaccgca atacccggaa
    34321 aggtttcgca aagctgcccc agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc
    34381 ctaccgccag aatattggtc agtttcgacg gcaatacggc atctgccgcg ccgcgttttt
    34441 gcaccaccag atggcaatcg cccatcttca gcagtgcagg taaagcgtca tacgattgca
    34501 gcgggaaaaa ttgcatgttg cgcagtccac gctgctgcgc cattttttcc agccgcgctt
    34561 tgccgccgcc ctgcccgaca atggcaaaaa tcagcggttc atcgcgcagg agatcggcag
    34621 cttcaataac gttttccagc ccctgctttt caccaatatt gccggagtaa agaatgattt
    34681 ttttgttatc cggcaggcca agctggttac gaagggcatc aacatcggcg tctgcaacat
    34741 gctgaaaacg ggcaatttcc gaccagttgg ggaagaagat gacgttatcc gccgccacgc
    34801 ctttttcgat ggctttattc atcatcgaac gcgaaatcgt ggaaacgtta tcgacgttat
    34861 gcagtccgct acgttcgaac gccgttgcca gctgtgccac tttgccgcct ttgccttttc
    34921 cggcaaggcc cagccccagc atggcgtcca cttcgtaatc ctgaatatgc agcacggtac
    34981 gcgcaccaga gagcttcgcc agcaggcgca ttcccggcgt gcaaaacagc gttggcacta
    35041 cgccaataat gcgatccggc ttccagcgac gttgcgccat cagtggaaag aaactgctga
    35101 cggcaaaact gccgagatgc aacaagcgtt tcagggtgct cggctgtttt ggcacgtaca
    35161 gcgggcagcg ccacaccgtg gcggcccctt cttctcgttt gtagcgccag gcggaatagt
    35221 tctcacccac ctgccattgc gggtagtaag gcggtgcggt aatgacccgc acctcatgac
    35281 cttgtgccgc caaccattcc accatctcgc cggtgtattt gccgatgccg gttaactccg
    35341 gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg cacgcttctc
    35401 agcgagaaaa taggctcggc tgttagcgtg aacatcgtta ctggcgagca gcgcgtccgg
    35461 cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagctctt cttccgctac
    35521 tctgaagcga aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg agaagttatc
    35581 gtcataaaaa tgctgccaga caccgtaaaa ctggcctgct gttatcggca gacgcagccc
    35641 cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt tctgcacgcg
    35701 ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc caagcagaaa
    35761 ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg agcgcactac
    35821 agtggcaaag tcttcctgac gtaaaaacat cattcccccc gaaagcggtc ttgattctca
    35881 aggaaccact ggtaagtgct ggcaagcccc gcttccagtg agatttcgtg ataccagcca
    35941 agctgatgca ggcgcgtcac atccagcagt ttgcgcggcg taccatccgg tttgctggca
    36001 tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc cagctcgcgg
    36061 atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggttg ggtattctcc
    36121 agccagactt catgcgccag ctccatgaca tgaatgctcg ccgccgccat atcatcgacg
    36181 tgcaggaatt cacgcatcgg tgtaccgctg ccccatacca ccacgtccgg cgcattctgt
    36241 gccgtcgcct cgtggaagcg acgcagcaat gctggtatca catgggagtt actcgggtgg
    36301 aagttgtcat gcggcccgta caggttggtc ggcatgacag tgcggtaatc gcgtccatac
    36361 tggcggttgt aagattcgca cagtttgatc ccggcgattt tggcaatggc gtaaggctcg
    36421 ttggtcggct ccagcgtgcc ctgcaataac tcgctttctg ccatcggctg ttttgccagt
    36481 ttcgggtaga tgcaggacga tccgagaaac atcagtttgt tcacgtcgtt ctgatgcgcg
    36541 gcgtgaatga tgttgctctc aatcatcatg ttctggtaga tgaaatccgc cggataggtg
    36601 ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc aatgcgttcg
    36661 ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt tcagttcatc gcgggtacgt
    36721 aataccagct ccacatcacc gcgctgttcg agctgccgct tgatggcaga accgaccatc
    36781 ccacaatggc cagcga
//
LOCUS       DAJVPT010000030        36038 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVPT010000030 DAJVPT010000000
VERSION     DAJVPT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..832)
                     /locus_tag="OQM47_001459"
     CDS             complement(<1..832)
                     /locus_tag="OQM47_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1479671.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     H"
     gene            complement(979..1055)
                     /locus_tag="OQM47_001460"
     tRNA            complement(979..1055)
                     /locus_tag="OQM47_001460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1019..1021),aa:Pro,seq:tgg)
     gene            complement(1098..1184)
                     /locus_tag="OQM47_001461"
     tRNA            complement(1098..1184)
                     /locus_tag="OQM47_001461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1148..1150),aa:Leu,seq:cag)
     gene            complement(1205..1280)
                     /locus_tag="OQM47_001462"
     tRNA            complement(1205..1280)
                     /locus_tag="OQM47_001462"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:His,seq:gtg)
     gene            complement(1339..1415)
                     /locus_tag="OQM47_001463"
     tRNA            complement(1339..1415)
                     /locus_tag="OQM47_001463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1379..1381),aa:Arg,seq:ccg)
     gene            complement(1518..2903)
                     /locus_tag="OQM47_001464"
     CDS             complement(1518..2903)
                     /locus_tag="OQM47_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1479672.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3094..3834)
                     /gene="rffM"
                     /locus_tag="OQM47_001465"
     CDS             complement(3094..3834)
                     /gene="rffM"
                     /locus_tag="OQM47_001465"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1479673.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(3837..5189)
                     /gene="wzyE"
                     /locus_tag="OQM47_001466"
     CDS             complement(3837..5189)
                     /gene="wzyE"
                     /locus_tag="OQM47_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1479674.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5186..6265)
                     /gene="rffT"
                     /locus_tag="OQM47_001467"
                     /gene_synonym="wecF"
     CDS             complement(5186..6265)
                     /gene="rffT"
                     /locus_tag="OQM47_001467"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1479675.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6262..7512)
                     /gene="wzxE"
                     /locus_tag="OQM47_001468"
     CDS             complement(6262..7512)
                     /gene="wzxE"
                     /locus_tag="OQM47_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1479676.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7514..8644)
                     /gene="rffA"
                     /locus_tag="OQM47_001469"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7514..8644)
                     /gene="rffA"
                     /locus_tag="OQM47_001469"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1479677.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8649..9323)
                     /gene="rffC"
                     /locus_tag="OQM47_001470"
                     /gene_synonym="wecD"
     CDS             complement(8649..9323)
                     /gene="rffC"
                     /locus_tag="OQM47_001470"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1479678.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9301..10182)
                     /gene="rfbA"
                     /locus_tag="OQM47_001471"
     CDS             complement(9301..10182)
                     /gene="rfbA"
                     /locus_tag="OQM47_001471"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1479679.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10201..11268)
                     /gene="rffG"
                     /locus_tag="OQM47_001472"
     CDS             complement(10201..11268)
                     /gene="rffG"
                     /locus_tag="OQM47_001472"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1479680.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11268..12530)
                     /gene="wecC"
                     /locus_tag="OQM47_001473"
     CDS             complement(11268..12530)
                     /gene="wecC"
                     /locus_tag="OQM47_001473"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1479681.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12527..13657)
                     /gene="wecB"
                     /locus_tag="OQM47_001474"
     CDS             complement(12527..13657)
                     /gene="wecB"
                     /locus_tag="OQM47_001474"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1479682.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13713..14759)
                     /gene="wzzE"
                     /locus_tag="OQM47_001475"
     CDS             complement(13713..14759)
                     /gene="wzzE"
                     /locus_tag="OQM47_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1479683.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14771..15874)
                     /gene="wecA"
                     /locus_tag="OQM47_001476"
     CDS             complement(14771..15874)
                     /gene="wecA"
                     /locus_tag="OQM47_001476"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1479684.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16114..17373)
                     /gene="rho"
                     /locus_tag="OQM47_001477"
     CDS             complement(16114..17373)
                     /gene="rho"
                     /locus_tag="OQM47_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1479685.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17458..17559)
                     /gene="rhoL"
                     /locus_tag="OQM47_001478"
     CDS             complement(17458..17559)
                     /gene="rhoL"
                     /locus_tag="OQM47_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1479686.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17700..18029)
                     /gene="trxA"
                     /locus_tag="OQM47_001479"
     CDS             complement(17700..18029)
                     /gene="trxA"
                     /locus_tag="OQM47_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1479687.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18160..19425
                     /gene="rhlB"
                     /locus_tag="OQM47_001480"
     CDS             18160..19425
                     /gene="rhlB"
                     /locus_tag="OQM47_001480"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1479688.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19559..21043
                     /gene="gppA"
                     /locus_tag="OQM47_001481"
     CDS             19559..21043
                     /gene="gppA"
                     /locus_tag="OQM47_001481"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1479689.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21090..23111)
                     /gene="rep"
                     /locus_tag="OQM47_001482"
     CDS             complement(21090..23111)
                     /gene="rep"
                     /locus_tag="OQM47_001482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1479690.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23198..23479
                     /gene="ppiC"
                     /locus_tag="OQM47_001483"
     CDS             23198..23479
                     /gene="ppiC"
                     /locus_tag="OQM47_001483"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1479691.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23525..25000)
                     /gene="ilvC"
                     /locus_tag="OQM47_001484"
     CDS             complement(23525..25000)
                     /gene="ilvC"
                     /locus_tag="OQM47_001484"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1479692.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25150..26040
                     /gene="ilvY"
                     /locus_tag="OQM47_001485"
     CDS             25150..26040
                     /gene="ilvY"
                     /locus_tag="OQM47_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1479693.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26037..27581)
                     /gene="ilvA"
                     /locus_tag="OQM47_001486"
     CDS             complement(26037..27581)
                     /gene="ilvA"
                     /locus_tag="OQM47_001486"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1479694.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27584..29434)
                     /gene="ilvD"
                     /locus_tag="OQM47_001487"
     CDS             complement(27584..29434)
                     /gene="ilvD"
                     /locus_tag="OQM47_001487"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1479695.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29499..30428)
                     /gene="ilvE"
                     /locus_tag="OQM47_001488"
     CDS             complement(29499..30428)
                     /gene="ilvE"
                     /locus_tag="OQM47_001488"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1479696.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30448..30711)
                     /gene="ilvM"
                     /locus_tag="OQM47_001489"
     CDS             complement(30448..30711)
                     /gene="ilvM"
                     /locus_tag="OQM47_001489"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1479697.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30708..32354)
                     /locus_tag="OQM47_001490"
     CDS             complement(30708..32354)
                     /locus_tag="OQM47_001490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1479698.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32357..32407)
                     /locus_tag="OQM47_001491"
     CDS             complement(32357..32407)
                     /locus_tag="OQM47_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479699.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32493..32591)
                     /gene="ilvL"
                     /locus_tag="OQM47_001492"
     CDS             complement(32493..32591)
                     /gene="ilvL"
                     /locus_tag="OQM47_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1479700.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32943..34422
                     /locus_tag="OQM47_001493"
                     /pseudo
     CDS             32943..34422
                     /locus_tag="OQM47_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34447..34785)
                     /gene="maoP"
                     /locus_tag="OQM47_001494"
     CDS             complement(34447..34785)
                     /gene="maoP"
                     /locus_tag="OQM47_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1479701.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34904..35743
                     /gene="hdfR"
                     /locus_tag="OQM47_001495"
     CDS             34904..35743
                     /gene="hdfR"
                     /locus_tag="OQM47_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1479702.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35839..35914)
                     /locus_tag="OQM47_001496"
     tRNA            complement(35839..35914)
                     /locus_tag="OQM47_001496"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35879..35881),aa:Trp,seq:cca)
     gene            complement(35923..35999)
                     /locus_tag="OQM47_001497"
     tRNA            complement(35923..35999)
                     /locus_tag="OQM47_001497"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35963..35965),aa:Asp,seq:gtc)
BASE COUNT         8803 a         9860 c         9088 g         8287 t
ORIGIN      
        1 gatggcaaac cgcacccggc gcaccgacaa aattggcaaa cgcccattcg atattcatca
       61 cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa aatcgccacc agaaattcac
      121 caaattcatc ggggcgcaca gaccattccg tcagttcccc ctgaatatca ccaggcgcat
      181 gtagtttcag tccatcgcgg acagctgttt catcagccag gcgctcgacc accggaataa
      241 actggatgaa ttcgactccc gcatcgcaca aaaaatcata aacctgtaac ggttgctgcg
      301 cgctggtgcg gttgacgcag accagcacgt tatagtcgac atgatgtttt tgcaggagcg
      361 tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc tttggtcacg cgatattgat
      421 tgtggatctc agccggacca tccagcgata acccaacaag aaaattattt tctgccagaa
      481 atgcacacca ttcgtcatcg agcagcacgc cgttagtctg gaaactgtta cttatcttcc
      541 tgccagcacc atatttcgcc tgtagcgcca cggcacggcg gtaaaactcc agcccgagta
      601 gcgttggttc gccgccctgc caggtaaaag ccacttcgtt ttgcagttcg ctggcggcga
      661 tatagtgacg gatatacgct tccagcgtgt catcgtccat atgcgtgact ggcttttcgc
      721 ggtaaaggga ttgtttttcg agataaaaac agtagtcaca gttcagatta caatcggaac
      781 cgctcggttt cgctatcaca tgaaaagcac gcgttggaac ctgttgcagc atggtcgctc
      841 cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc ataccgtggg gcaatgagtt
      901 attacgggcg tttattgcga agggagggtg acgaaatgca cagaaaacaa aaaaccccgc
      961 cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga acctccgacc cactggtccc
     1021 aaaccagttg cgctaccaag ctgcgctact cgccgaaata ctgctttttg aatttttagt
     1081 tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca
     1141 ctaacacctg aagctagcgc gtctaccaat tccgccacct tcgcattgtc acaacttcta
     1201 ataatggggt ggctaatggg attcgaaccc acgacaactg gaatcacaat ccagggctct
     1261 accaactgag ctatagccac cactacaaat cttgttacgc ggtattacta ccaccgcagc
     1321 tcaagcgctg ggactaaatg gcgcgcccga caggattcga acctgagacc tctgcctccg
     1381 gagggcagcg ctctatccag ctgagctacg ggcgcttagc gccgttgcgg gggtggataa
     1441 tacggacttc ccacccctct gtctagtccc tttttaaata aattgctcgt ttggttacgg
     1501 tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt taccgtggcg attaagccca
     1561 aaaactttat aaatcgccgt caccgccagc ataaagatga tgccgacaaa cagcgacata
     1621 cgcgtatctt cattaaagta catgccgatc aaaacgcaaa tcaggaatgc cattgttacg
     1681 tagttggccc acgggaacag gattgagcgg aacggatggc tggcaatcgc cgctttatgt
     1741 gcacgccgaa aacgcagctg gcttatcaat atcacaaacc atggcaccat ccccggaagc
     1801 acgctggcac tgtagacgta gacaaacaca cgctgcggat tgggaatgat gtagttcagg
     1861 catgagccaa ttagcagaat agcaatagat actgccacac ccgcaaccgg tacgccgtga
     1921 cgggaaactt ttgccattgc cgccggtaac tgacggtttt tcgccagtgc gtagagcata
     1981 cgtccgcaac tgtacatgcc gctgttacag ccagagagcg cagccgtcag caccacaaag
     2041 ttgataatgc ccgccgctgc ggtaataccg attttggcaa aagtcagtac gaacgggctg
     2101 ccgttgctgc caatttcatt ccacgggaag atggtgacga taacgaaaat cgcgcctacg
     2161 tagaaaatca ggatccgcca cagcactttg cctacggcac tgcgcagcgt cacctgcgga
     2221 ttcttcgctt caccggcagt aatgccaatc agctccacgc cctggtagga cgccaccaca
     2281 atacacagag cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc atgctctgtg
     2341 agattgctaa aaccaatcga ctgcccgcca ttgccaaagc caaagaaaat cacgcccaag
     2401 ccaatgacaa tcatcacgat aatcgtggtg actttgatca tcgcgaacca gaactcgatt
     2461 tcgccgtaca accgtaccgc cgccagattc gccaacgcca ccagcgccac cgcgatcaat
     2521 gcgggtatcc actgcgccat ctccggggac caaaactgga cataaacgcc aatggcggtg
     2581 atttctgaga tccccaccgc catccacata aaccagtaag accaggcggt gagatagcca
     2641 aaaaacgggc tcatataacg atgcgcataa acggcgaacg aaccggtaac cggttcgagg
     2701 aacaacattt cgcccattga acgcatgatg aaaaaaacga acagcccggc gatgatatag
     2761 gccaacaata cggatggccc ggcccatttc agggtactgg cggcccccat aaacaggccg
     2821 acgccaatgg tgccccccag ggcgatgagt tcgatatgtc gagcttccag cccacgctgt
     2881 agctctggtt tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt tccggtgtta
     2941 ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca ggagaaatgg
     3001 caaataaagc attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca ggcgacgaag
     3061 tgaccactcc gtcgctttac aaagagagga aaatcagagg ttgccggtgt agtgccagcg
     3121 taaataacgc agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa gcaggcggta
     3181 gagccactcc agtcccagcg tttgccagat tttcggtgcg cgttttacgt gaccggtgaa
     3241 aacatcgtaa gtcccgccaa cgcccatata cagcgcatct ggatgcacca gacggcagtc
     3301 gcgcatgaag atctcctgct ttggcgatcc catcgcaacg gtgacgattt gcgctccgct
     3361 ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac catcctgact
     3421 gccaacgata ttcacattcc attggttgcg cagtttagct tcagtttgcg ccagcacttc
     3481 aggtttaccg cccacaagaa ataccggcgt cccttctttg cctgcgcgcg ccatcagctc
     3541 ttcccagaga tcggcaccgg caacgcggga aacctgcgcc tgcgggtact ttttacgtac
     3601 tgaacgtaca atgctgatgc catccgcata tttaaattcg gcagcgttaa ttaactccct
     3661 gacctcggcg ttatcttcaa tggtcagcat tttttcggca ttaatggcaa ccagcgttcc
     3721 ctgcttaagc tgcccgtcag caaacagata atcgagggca tgctgcatat cacgccaacc
     3781 aatcaactgt aagccacgca gcgtataggt cggtgccgtg gtgttgttat tcattgttat
     3841 ccttcaacct gcgtccggag cgatgatttt gtacgtttat gaatgagtcc ggcgctttca
     3901 aaaagccagt acaacagttt tgcgatcatc agacatgcgc cgaagaccac gataaaaaag
     3961 actacgcgtg agacaaacga atccagccct tcacgtgcca gcacgatcat attgaagatc
     4021 gccccaaagc agaaactgtg caatatcgca gccttatagc gattagtctc gcggttgccc
     4081 agctcataca gccagtcgaa ccatttgatg atcagtccaa ccacaatcgc cccgagtggg
     4141 atgaacaacg cgccgcccat caccaccagt gagcctataa gcgtaggcga gatcgccagt
     4201 ccggagtggt tattcagtac ttcccaggta aagtaattgg ctgagttcag caccatactc
     4261 gggcgccccg gccacagcca ggaagggata aagacataga aatcgcggac aattggagcc
     4321 aggccctgga agtcgatgtt gtcgtagttc tgcaacagca acgccagatt ctcccacggt
     4381 gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg cttcatcgcc gctcacattc
     4441 attccatagc gttttaacgc cagccagaac atgccaacaa tccccagcac gcccgccgcc
     4501 gccagcatcc acaacgaaat ccagccgcga ataatgccaa taaacaggaa gatagcgaat
     4561 gcgatgatga tattggcgcg agtgccgccg acaatcatat aagtcagcaa gccaaaggcg
     4621 accgtgctga cgaggaaaaa cagccacgct ttgctgtcct ggcgcagaaa gtagaccacc
     4681 agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg ccacgccgga gacttcactg
     4741 gaaaagatct gactgtagga gttaagccgg aacagcaaaa agccgttgtg catgaagaag
     4801 atgccgacgc ttaccagcgc gatacccatc aggatcaccc acgtaagatt ggtctccacg
     4861 cggttcatgg taaacagcgg acggcgcggt acatcagcaa cgcgtttgcg taggcgggtt
     4921 ttgtaggtga catagtaaac cgcgtagaag cagcccgcag aaagcaacac ctgcaacaag
     4981 atttctggcg gcgcgacacc aacatcaaag cgaaatacca gcacgctggt cagcgggaag
     5041 ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt aaagcgcaca
     5101 cggcgaaact caaaccaggt cagcgtggca ataaacagcg tgcagagcag ccagaccaca
     5161 aacaggccac tgaattgcag cagactcatg cgacctcccc ggcggcaatc gccaacgccc
     5221 gctgccagcc ttgtagatag ttagggctaa agaaggcaat ggtgttttta tccgccgacg
     5281 ccaactggcg ctgcgcttca cgcacaatat cctcgttgag atcgtcggta gtaaacagca
     5341 ctggcaaatg ctgttccgtc atatcctgcc agaacggatt ttccctgtta agcacacaag
     5401 gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg gcaaaaataa
     5461 agtaaccaag atcgcactga cgaagtagcg ccagataggc gtcaaattcc agtttttcgc
     5521 tcaaaatttg cagattttct tcgctgaata actccagccc cgcctgacga acttcctcaa
     5581 tgtacgcttc gttattaggc ggatatccca tcggcaccac cacttttacc gtatcgccaa
     5641 attgctgatg aacggcacgt aaagcggcga catgctcatt gctgcggtcg ccggagttcc
     5701 ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt cgccatcgta ttgagagaag
     5761 ggtccatccg cgtcgggaag tacagcagtt ctccccgcac ctttgggtgt gttttggcaa
     5821 aaaagctcaa atcgccgcgg gtggcaaata cacagccgac tcgcttttgc gccaggcgac
     5881 gtagtgggta aaaaagctta tatctcaagc cactggaaag ctcatacagg tctgcccccc
     5941 agatatgcca gaaaaactgg ctgggcttaa tgccaccact cagcagagcc agccacagtg
     6001 tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg gttagctttt gctttcgcga
     6061 tgaccgcttc cgccagcgat tttttcccag ggaaaaattg cacagaaagc gccggacagc
     6121 tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc tcgctcgtcg
     6181 cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt atggtgaggg atatccgatc
     6241 ccagtacgtg aatcagtaca gtcatgcccg cctacgccag agtaaaaaca cgccacaaca
     6301 aagagaaaaa tagacgatat aagttgccat atatgcctgc gccgcgccca gtgcaccatg
     6361 cgcagggatt agccagtggg caaataccat caataaagtg aactggctga cttccgccag
     6421 aatataaaac cgcagtgacg ctttggcgat caccagataa ccaaagacat aagcgcccac
     6481 ttttaacaca tcacccacta actgccaggc aaagagatcg cgcatagcgg taaatttatt
     6541 cgacaacagc aaccagatag caaaatcacg cagcaaccag acggtaaaac tcgctgccgc
     6601 cactgccggt aagacgaatt tcagcgattt aaccacttcc cgggtgatat cgcgcttttc
     6661 cgttagccgc gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa taaattgcag
     6721 gtaggcatcg gaaatactgc tcaccccttg ccagatcccc acttcatccc agctatactg
     6781 cgccgccagc agtttacgca tcatgatgta agcaacaggc aaggtcaccg acgtaatcaa
     6841 cgccatgagc gtaaatttgc tcaactgccc tgccagaccg ttatcccagc tgggtttcag
     6901 atagcttaac gggatgacac cacgtttaat caacatgatg gcggcaggaa ttaccaccag
     6961 cgcgggaatc agcgccagac ccagcaacgc cccttcataa ccgcccaaac ggtaactgac
     7021 gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc gataacgcat tacctgcggc
     7081 atcgcgaaag cctttcatca gcgccagtaa caggttgccc caggcgatcc ccatttgcac
     7141 cagcgccact aaacgcacca gcccctgata gtcggtatta ccaaacaatc cctggctgat
     7201 tggcgcagct gccagcacaa aaaccagcgc catcagcgta gagaaaccaa gtaccatcgc
     7261 tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta tcatggtact gggcaacgta
     7321 tttggttaca ccgttaaaga tgccagcccc ggcaagcacg ccgagcacgg taatcaactg
     7381 gcggaaattt gccgccagcc caagccccgc cggaccaaat gacaccgcca gcaacttacc
     7441 gaccagtaac ccggcaccaa tcttgaccag tgtactggcc gccgtccaca aggacgcttt
     7501 tgccaacgac atatcaggag aagtagttca acaaagtcgc aattaccgta cgctgattga
     7561 cgggcgacag gttgtagaac agcggcaggc gcagcaggcg ctcgctctct ttggtggtgt
     7621 agcgatcttc accgtggaac tcaccaaagt gttcccccgc agggcaaccg tgcagcggaa
     7681 tgtaatgaaa caccgccatg atttccgctt ctttcagaaa gttaatcaac gcgctccggt
     7741 catcaatatc ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag ccatcgggaa
     7801 tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc taacgtatcg tagtagtttt
     7861 gccacagcgc cagacgttgc tggttgatac gatccgctgc ttccagttgc gcccacaggt
     7921 acgcagcttg cagatcggac atcaaatagc tggagccaat atcgcgccag gtatatttat
     7981 cgacctgacc acggaagaac tggctgcggt tagtgccctt ttcacggatg atctcggctc
     8041 gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc ttcaccaccc gccgtgtagt
     8101 ttttggtttc atggaagcta aagcagccaa tatgaccaat ggttcccagt gcacgccctt
     8161 tgtaagtgga catcacgccc tgagcggcat cttccaccac aaacaaatta tgctttttcg
     8221 ccaacgccat aatggtgtcc atttcgcagg ccacacccgc gtaatggacc ggcacgataa
     8281 cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc gtcgatgttc atggtgtccg
     8341 ggcgaacatc cacaaagacg attttcgcgc cacgcagcac aaaggcattg gcggtggaga
     8401 caaaggtgta gctcggcatg atcacttcat cgccaggctg gatatcgagc agcagcgccg
     8461 ccatctccag cgaagcggtg caggacggcg tcagtaacac tttggcgctg ccaaaacgtt
     8521 gctccagcca ctgctggcag cgacgggtaa aaccgccatc gccacacagt ttgccgctac
     8581 ccattgccga ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg ttaaatggaa
     8641 tcatgtgatc acctgtataa ccagtacgcg gtgctttcta cattcgcacc actttgtatg
     8701 tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca cccgcaaagt tgttttaccg
     8761 cgagcatacg cccagtttag cgccgtttgc atcagctcag cacctgcacc gcgtccagcc
     8821 agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta aagagacata gccgcgaata
     8881 tcgccggacg ccgcacgtag aatcagacat tgatgatcaa aggtgccgcg cacggcattt
     8941 tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg cataccacgg cgcacgaaaa
     9001 cggctttgcg caaatgcggc gctagctaac tgacgtaatg cgggaatatc ggtctcttgt
     9061 gccacgacag caccgctatc actgacattg ttcacgggta gcgccaaatc aacttcacct
     9121 tctaccaggg agaatcccag ctgttgcagg gcatccagtt cccccgtatt tgatgccgca
     9181 attttggcct gcacccgtga ccatggcgct aaggcgtctg acgtcaggag cggtgcttca
     9241 gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg cgttttccca ggttagtggc
     9301 tcaatactgg cgcggacggg cacgaagtaa ctccagcaga tattggccgt agccagtttt
     9361 cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg agccagccgt tacgccaggc
     9421 gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt tctactgtct gcacaaaagt
     9481 gctggcctca atcaggctgt cgtgagtgcc ggtatccagc caggcaaatc cgcgcccgag
     9541 cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg taatctccag
     9601 ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc acgactttac tgtcgtagaa
     9661 ataaagcccg gtcaccgccc agtttgactt cggctgtttt ggcttttctt caagcgagat
     9721 agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt tccgggtcca tcacctgata
     9781 gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga cgcagcttcg gactgaagcc
     9841 ctgaccgaag aagatgttat cgcccagcac cagacaagaa ggttcaccat taaggaaggt
     9901 ttcaccgatg ataaaggcct gcgccagacc gtccgggctg ggctgttcgg catattccag
     9961 ctggataccg aactcactac catcgcccag caggcgctgg aaataacctt tatcttccgg
    10021 cgtagtgatg atgagaattt cgcggatacc ggccagcatc agcaccgaca gcggatagta
    10081 aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg taatcggatg
    10141 caatcgggta ccggaaccgc ccgccaggat aatacccttc atgtttgcct ccgctgaaaa
    10201 ttagcctttc agacctaaac gctcaccctg atagctgccg tcctgcacct gcttccacca
    10261 gctttcatta gccagatacc actgaaccgt tttacgcatt ccactttcaa aggtttcctg
    10321 cggcagccag ccaagttcac gggcaatttt cgaagcatca atggcataac gcagatcatg
    10381 ccccggacgg tcagcgacaa aggtgatcag gtcacgatag tacgccacgc cgtgcggctt
    10441 gttcgaagcc agttcttcca gcagctcgca aatggtttcc acaacatcga gattcttacg
    10501 ctcgttgtga ccaccaatat tataggtttc accgactttc ccggtggtcg ccacgcaata
    10561 cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg atttgctgcc cgttgccata
    10621 taccggcagc ggtttacccg ccagcgcgtt gaggatcatc agcgggatca gtttttccgg
    10681 aaagtggtaa ggaccgtagt tattcgagca gttggtgatc agcgtcggca gaccgtaggt
    10741 ccgcagccag gcgcgcacca gatggtcgct gctggctttt gacgcggaat aagggctgct
    10801 cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt caccatatac
    10861 ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt ttatcttccg tcagcgtatt
    10921 ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg acaatgttgg tttcaataaa
    10981 cgctgccggg ccgtcaatag aacggtcaac atggctttcg gctgccagat gcatgacaca
    11041 gtctggctga tgctcagtga atacgcgtgc cagttctgcc cgatcgcaga tatcaacttt
    11101 ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc gacatcaggt ttccggcgta
    11161 ggtcagctta tcgaccacta ccaccgcatc gctcgtttcg ttgatgatat aacgcaccag
    11221 cgccgagcca ataaacccgg caccacctgt tatcagaatt tttctcatca gcgccagact
    11281 cctttggcat cgacgacata ctgctgatgg acattgtcgc cattgataac tttgaactga
    11341 ctatgatcga ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc aagctgcgcc
    11401 agagtacaaa gcccggtcag ttttttcggc aactggtgga tgttaggctc aacaaccagc
    11461 gtttcgccgc tgtgccactg ggcgatcagt tcagcgattt ccatcgccgg gctttcgcgc
    11521 aggtcatcaa tattcggttt aaacgccaga ccaaagcagg cgattttcag ttcactggct
    11581 cgtttatcgg tagcagcaag gcagtcggca accgccgctt tcacctgatc gataacccag
    11641 aacggtttgt gatcgttaac ttcgcgcgcg gtacggataa gccgcgcctg ctgggggttc
    11701 tgtgccacga taaaccacgg atcaacagca atgcagtgac cgcccacgcc agggccaggc
    11761 tgaagaatat taacgcgagg gtgacgattc gccaggcgaa tcagttccca gacgttaatc
    11821 ccctgatcgg cacaaatcag cgacaattca ttagcaaaag cgatattcac atcgcggaag
    11881 ctgttttcgg tgagcttaca catttccgcc gtccgcgagt tagtgacgac acactcacct
    11941 tcgaggaaaa ttttgtacaa ttcgctggcg cgggccgaac aaaccggcgt cataccacca
    12001 atcacgcgat cgtttttaat cagctcgacc ataacctggc ccggtaatac acgttccggg
    12061 cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct gtgggaaagt cagatccggg
    12121 cgcatctctg ctaaccattc tgccatcttc tcggttgacc ccaccggcga ggtggattca
    12181 aggatcacca gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc cgattcaacg
    12241 taggtcatat ctggctcatg atcgccttta aacggcgtag gtacagcaat cagccaggca
    12301 tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc agtttttact
    12361 acgctcgcca aatcaggctc gacgatatgg atttcgccac gattgatggt atcaaccgcg
    12421 tgttggttga tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc tgctgctgta
    12481 ggcagcccga tgtaacccag tccgataaca gaaatggtcg caaaactcat agtgatatcc
    12541 gattattttt taacgcttcc agaatgcgag agcatgcctg accatcacca tacgggttat
    12601 gggcgcggct catagcttga tattcgtttt cgtcttttaa aagacgcgtc acttcctcga
    12661 caattcgctg cttatccgtg cctaccagac gcaccgtacc cgccgtcacc gcttccggac
    12721 gctcagtggt atcgcgcatc accagcaccg gtttccccag cgaaggcgct tcttcctgaa
    12781 tgccgcctga gtcagtcaaa atcagccagg cgtggttcat cagccagaca aacggtaaat
    12841 actcctgggg atcgatcaga atgacatttt tcacatgccc cagaatgcga ttgaccggct
    12901 ctctgacgtt cgggttgaga tgcaccggat agacaatctg gatgtcctgg tgcgtggtgg
    12961 cgatgtctgc cagcgcgtgg cagatttctt caaagccacg accgaaactc tcacgcctat
    13021 gaccggtcac cagaatcatc tttttatcgg gggcgataaa cgggtaattt gccgccagtt
    13081 ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac ccataacagt gcatcaatga
    13141 ctgtattacc ggtaatgaag attcggctat ccgcaacgtt ttcacgcagc aagttttgcc
    13201 gggaagtttc ggttggagag aagtgataca tcgccagatg cccggtcaat gtacggttag
    13261 cctcttccgg ccacggcgaa tagagagcgc ccgtgcgcag accagcctca acgtgaccaa
    13321 caggaatacg ctgataaaac gccgccaggc tggttgccag cgtcgtcgtc gtatcgccgt
    13381 gaaccagcac gacgtctggt ttgaactcgg caagaatagg ttttagccct tccagaatcc
    13441 gacaggttat ctctgtcagg ccctgtcctg gctgcattat gttgagatcg tagtcaggta
    13501 caatggaaaa gagtttcagt acctgatcaa gcatctcccg atgctgcgca gtgacgcaaa
    13561 ctttagcctc aaaaaaagga tcttttgcca acgcatgcac caacggagcc atcttgatgg
    13621 cttccgggcg cgtaccaaat acagtcagta ctttcacatc gattctcttc gaataagcgg
    13681 cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg ggttaatgcg
    13741 acaccagccc cgatcagccc cccgacaatg ccccacataa tcatcaggaa ggcacgacgt
    13801 gggctatcgc gttttaccgg ttcttccggc gtacgcaaat agcgataggt ctgaaaacgc
    13861 ggatccaggg ttggaccaac attcagggtg tttaacatgg ctcgattctg atcatagtcg
    13921 agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc gagcctggag cattggacgc
    13981 ccaagcagga acatttctga atcaggtaat tcctcggcag gcacatctgt cgcactgcgc
    14041 gaaatattat gctgctcagc aattttcagc gcctgctcaa tactgttcat ccggcggtcg
    14101 tagatggctt tcgccacctc ttcctgacgc ttcacctgag ctttcatctg gatggtgcgt
    14161 gccgcccatg cgcctttcag ctcatcattc agatggctgg ctgcacgctg gctggcaaaa
    14221 gcaacatact gacgtaacag gttattagcg tcaggcgcgg tttcggcaat aagcttcacg
    14281 ctgtcattga ccgcgcgagt aaagtctccg gggataaact ggatgttgtt aatcatttca
    14341 tccagcaacg ccgcatcggc tttgctgttg cccaccatcc gctgtttgta atagtcggtt
    14401 tgcagccaga actctctgcg ggtatcccac gaggccagtt gcataacaaa ctctttatag
    14461 gcttcgtcca tgaccgatgg ttgatcggca gaagccatgt ttgaacggac atccaggtta
    14521 cgcaaaaatt gctgctgcga gtaatatccc cccagcatat tcaccgttgg acgatcggta
    14581 atcgccgtcg agctccactc ctgacgagca aaaaaagtat acgccagcgc gattaacgca
    14641 aacgccagcc ccatgccaat aatccatagc ttcccagccc acaaggtacg aaacaaccca
    14701 cgaatatcca gttcattttc agcgtcttcg gccggtttcc caggcattgg ttgtgtcatc
    14761 acatcctcat ttatttggtt aaattggggc tgccaccacg atttctacgc agtctgcgtt
    14821 ttacgcgctt aataaagcga gcaactttcc aggcacgctt aatgcaatat ccatagagga
    14881 agaatgctag caaaaagagc accagcatga cccactccgg gacaaaatga gaatattctg
    14941 ccagcacgcc aatggaagcg agcagtgctg cggcaagggt aatcagcaca aacgcctgac
    15001 gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg ctgacggtca ggagagaatg
    15061 ggctcatgcc tttacgcagg cgacggtaca taatcgccac catatccatt agcggaatgg
    15121 cgattatcca caaagcggta accgggctga tgggatgggt tttgccctgg gtcgtttcga
    15181 gcaggatcca gataacggta aaaccaatca gcgtactgcc cgcatcaccc ataaagactt
    15241 tgtagcggcg acccaggata ccaaggttaa gcatgatgta tggcaggatg gcggcgatca
    15301 tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa ccacaaaatc ataccgattg
    15361 ctgcaaacga gacgcaggac aacccgccca gcaagccatc aatgccatca accatgttga
    15421 acgcattaat ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac ggtccgagca
    15481 ccatctccca ggagccaaag atataaccca ggctactgag ataaagtttg ccgaacacca
    15541 tcataacaat gccaacagcg gcctgtatgg tggcacggat ttttacgctg atatcaaaac
    15601 gatcatccag cgcgccaatg aaaacaagca caccggcaca agcgagatag agagatgcat
    15661 gcggaatata gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa accgaaatcc
    15721 ccccaacgag aggtatcaat ccctggtgac gtttgcggaa gtttggttta tccactaaac
    15781 cgactttttt tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat aaaaaaatac
    15841 tgatgagatc agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt
    15901 agcagaagta taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca
    15961 gccctgtgca aaaatcaacc atttgatatg caaattgcat aacatgccag caaattccag
    16021 gaattttcct aacatcgcca gcgcgacatt aagattacag atataaaagc aaaacgccac
    16081 gtaaacacgt ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa
    16141 atcgtcattg gtcttggtca tggccagttt attaatgagg aattccattg catcgatttc
    16201 gcccatcggg tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtcgt
    16261 gagcagctct tctttacggg taccggaacg gttgtagtcg atagccggga agacgcgttt
    16321 ttcagcgatc ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta
    16381 aataacttcg tccattttag aaccggtatc gataagcgcc gtcgcgataa tggtcaggct
    16441 gccgccctct tccacgttac gcgccgcacc aaagaagcgt ttcggacgat gaagggcatt
    16501 ggcgtccaca ccaccggtca acactttacc tgacgccgga acaacagtgt tgtaagcgcg
    16561 cgccagacga gtgatggagt cgagcagaat gataacgtct ttcttgtgct caaccaggcg
    16621 tttggccttc tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg gttcgtcaaa
    16681 ggtagaagca acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg
    16741 ttcgtcgatc agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgagc
    16801 aatgttctgc agcagcatag ttttaccggc tttcggcggt gccacaatca gaccacgctg
    16861 accacgaccg ataggtgatg ccagatccag tacgcgagca gttaaatctt cagtagaacc
    16921 gttaccacgt tccatacgca gacgagagtt tgcgtgcagc ggggttaagt tctcaaagag
    16981 gattttgttg cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc
    17041 aaaatagcgt tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg
    17101 gaggttgaaa cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta
    17161 ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacgccatc
    17221 accaaagata tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg
    17281 cttacgcatg cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga
    17341 aaccggcgta ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt
    17401 aaatctcgaa cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca
    17461 tcgactcatg tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga
    17521 cggattgagt gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt
    17581 tggaaattac aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta
    17641 gcatgactca cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct
    17701 tacgccaggt tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg
    17761 gttgccgcca cttcaccgtt tttgaacagc agcagagtcg ggataccacg gatgccatat
    17821 ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga
    17881 tattcgtcag cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct
    17941 gcccagaaat cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg
    18001 tcagtcaggt gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt
    18061 tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg
    18121 ctttcgtaaa gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca
    18181 gaacagaagt tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa
    18241 gggtttcata actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt
    18301 gacgtagccg ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg
    18361 tttcattatc ttctctctca tcctgcgatt gccgatcgca aggtgaatca accgcgtgcc
    18421 ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc acgccgacgc ggaaccgctg
    18481 gcggaagcta ccggcctgaa gctgggcctg gcttacggtg gtgatggcta tgacaaacag
    18541 ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca ccacgggtcg tttaattgac
    18601 tacgccaagc agaaccacat taacctcggt gccattcagg tagtggtact ggacgaagcc
    18661 gatcgcatgt acgatctagg ctttattaaa gatatccgct ggctgttccg ccgtatgccg
    18721 cctgcaaatc agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa
    18781 ctggcgttcg agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg
    18841 ggccaccgta ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg
    18901 caaacgctga tcgaagaaga gtggccagac agagcgatta ttttcgccaa taccaaacac
    18961 cgttgtgaag agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca
    19021 ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggcgatctg
    19081 gatattctgg ttgccaccga cgttgccgcg cgtggtttgc atattccggc agtgacgcac
    19141 gtctttaact acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca
    19201 ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt gtgaagagta tgcattgaat
    19261 ttgcctgcta ttgagaccta tattggtcac tcaattccgg taagcaaata caatccggac
    19321 gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc gcccgcgcac aggcaatggt
    19381 ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact
    19441 caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag cttactgtag
    19501 taagtgattc gggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat
    19561 gggttccacc tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct
    19621 ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaattaaac gcaaagtgcg
    19681 tctggctgct ggcctgaata gcgaaaatga cctctctaat gaagcaatgg agcgcggttg
    19741 gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt
    19801 tgtcgctacg gcgacattac gacttgccgt caatgcgggt gattttattg ccaaagcgca
    19861 ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcgc gtctgattta
    19921 tcagggcgtt gctcacacca ctggtggtgc cgatcagcgc ctggtggtgg atataggcgg
    19981 tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc
    20041 gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaagaaaa
    20101 ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg
    20161 gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat
    20221 catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca
    20281 gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg
    20341 tgcattagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat
    20401 tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca
    20461 tctggccgtc gagcaggata ttcgcagccg tacactgcgt aatattcaac gccgctttat
    20521 gatcgatatt gatcaggcac agcgcgtagc caaagttgcg gctaacttct tcgatcaggt
    20581 ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct
    20641 tcatgaaatc ggcctgagcg ttgacttcaa acaagctccg caacacgctg cttatctggt
    20701 gcgtaacctg gatcttcccg gttttacccc cgcacagaag aaactgctgg cgacgctact
    20761 gctcaaccag actaacccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccacc
    20821 gcgcgtcgca gaacaactct gccgtttact ccgcctggct atcatttttg ccagccgtcg
    20881 ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac
    20941 gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaaa
    21001 ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg
    21061 cgatgctgac gcatcttctc cggccttgat tatttccctc gttttgccgc catcatcgct
    21121 ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcact gaccactttg
    21181 cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc
    21241 gggcgcacca gttcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc
    21301 tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg
    21361 atactgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac
    21421 tccagccctt ttgacgcgtg gagagtcatc agttgcacct gatccagctc ttcttcactc
    21481 tcaccacgct ccatcatgtc gcgtaaagta aagcgcgtca ccacctgggt gagcgtcatc
    21541 ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg
    21601 ttcttcatgc gcatttcggc agctttcgtg ctgggcgatg tttcgtacag ccaggattca
    21661 taatccatgc cgtggatcag atcgcgcacc gcggcaatcg gctcccgctc cgccagacgc
    21721 tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc
    21781 gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc
    21841 cactcaccca gctttttcag cgtcgccggg ccaatctctc gcttcggcgt gttaacgata
    21901 cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc
    21961 ttgatttcag gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt
    22021 tgcatcagga atttttcaaa cacccgcgac tgatagttac cgcgataaag aatagcgtaa
    22081 tctttgtact gcgttttatt gacgaagtga tgcgcaatca gttcaccagt gacgcgctcg
    22141 gcttcatgtt cttcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg
    22201 gagaacagac gcttttcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc
    22261 aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa
    22321 tcctggctca gcagcaccag gttttgcgga cgtgcgccgc gccaggagta gatcgactgg
    22381 tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagttca
    22441 tactggctgg tgttggtgtc ctgatattcg tccaccagca ggtagcgaat tttgttctgc
    22501 cagcgctcgc ggacttcttc attgcgttgc agaagtagcg tcggcaataa aatcagatca
    22561 tcgaagtcga gcacattaca ggctttcagg tgtgcatcat acagcccata acaatgggca
    22621 aaaatacggt cccgctcgcc aatcgcactg gctgccgcct gggacggtgt tttgagatcg
    22681 ttcttccagt tagagatggt cgaaatcaac tgttgcagga gaactttgtc atcttcaatc
    22741 agcccttcgg tcaactcttt gagcaacgcc agctgatcgg tatcgtcaaa cagcgaaaag
    22801 ttcgccttca tccccagcgc cgcatactca cgcttgatga tatccagccc cagcgtgtgg
    22861 aaggttgaga tcatcagccc atgcgcctct ttgcgaccca gcgtctgtcc gacacgctct
    22921 ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa
    22981 ccgcacccgc ggatcagatg ggcgatttta ttggtgataa cacgagtttt accggaaccc
    23041 gcacccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg
    23101 tttagacgca taggaaaatc gctcaatgaa agtcgggagg ggaaaataac agcgtggtag
    23161 tatagccagc ctcatcctta ccactcaagg cacgatcatg gcaaaaacag cagcagcact
    23221 gcatatcctt gtaaaagaag agaaactggc tctggatctt ctcgtgcaga ttaagaacgg
    23281 tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg
    23341 tgatttaggt gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc
    23401 ttgtccggta ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa
    23461 ggtgctgtac cgtaactaat agcaaggcct tctccgggag aaggccttga actctgaatc
    23521 gctattaacc cgcaacagca atacgtttca tatccgtcat atagccgcgc agtttcttac
    23581 ctacttgctc aatcgcatgg ctgcgaatcg cttcatttac atcacgcagc tgcgcgttat
    23641 ctaccgcacc ttccggaata gctttaccca aatcgcccgg ttgcagttct gccataaacg
    23701 gtttcagcag cggcacacaa gcgtaagaga acagatagtt accgtactcg gcagtatcag
    23761 agataaccac gttcatttca tacagacgct tacggacgat ggtgttggca atcagcggca
    23821 gttcgtgcag tgattcatag taagcagatt cttcgatgat gccggaatca accatggttt
    23881 cgaacgccag ttcaacgccc gctttcacca tcgcgatcat cagtacgcct ttatcgaagt
    23941 actcctgctc gccgattttg ccttcatact gcggcgcggt ttcgaaggcg gttttgccgg
    24001 tctcttcacg ccaggtcagc agtttcttat catcgttggc ccagtccgcc atcataccgg
    24061 aggagaattc gccggagatg atgtcgtcca tatgtttctg gaacagcggt gccatgatct
    24121 ctttcagctg ttcagacaac gcgtaagcac gcagtttcgc cgggttagag agacggtcca
    24181 tcatcagtgt gataccgccc tgcttcagcg cttcggtgat ggtttcccaa ccgaactgaa
    24241 ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac cagcttgtcg aagcacagca
    24301 gcgagccagc ctgcaacata ccgcacagga tggtttgctc acccatcagg tcagatttca
    24361 cttccgcaac gaaggacgat tccagcacgc ccgcacggtg accaccggtt gctgccgccc
    24421 atgctttagc gatcgccatg ccttcgcctt tcggatcgtt ttccgggtga acggcaatca
    24481 gcgtcggtac gccgaatcca cgtttgtact cttcacgcac ttcggtgcca gggcatttcg
    24541 gcgcaaccat gactacggtg atgtctttac ggatctgctc gcccacttcg acgatgttga
    24601 aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag tggctgtacg gtgcgcacta
    24661 catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag atccgcctgt gggatcagtt
    24721 cttcgtaagt acccacttta aaaccatttt cggtcgcttt acgccaggac gcgcgcttct
    24781 cggcaatcgc ttctttacgc agagcgtagg agatatcgag accagaatca cgcatgttca
    24841 gaccctggtt cagaccttgt gcgccacagc cgacgatgac tactttttta ccctgaaggt
    24901 agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg acatttgccc agctgtgcca
    24961 gctgctgacg cagattcagt gtattgaagt agttagccat ggtgattcct cgtgatgttg
    25021 tgcttcttat tgttcggttc gcgtttgcga gattgaattc actatatgac aggaaattta
    25081 ttgcggaaat tgatatattc acaacgtcac attgcaattt ctgcaacgtc aacattgagg
    25141 gctgcgccat tggatttacg cgatctgaaa accttcctgc atctggcgga aagccgccat
    25201 tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca cgctctcacg gcagattcag
    25261 cgcctggaag aagatctcgg ccagccgctg tttgtgcgcg ataaccgcac ggtgacgctg
    25321 actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa cgctgttgca gtatcagcag
    25381 ctgcgccaca ccatcgacca gcaagggccg ttgctctatg gcgaattaca tatcttctgc
    25441 tcggtgaccg ccgcttacag ccatctgccg ccgattctgg atcgcttccg cgcggaacac
    25501 ccgtcggtgg agatcaaact cactaccggt gatgcggcag atgcaatgga aaaagtcgtc
    25561 acaggtgaag ctgatctggc gattgccggg aagccagaga ctctgcccgg cgcggtggcg
    25621 ttttcgatgt tggagaatct ggcagtggtg ttgattgcac ccgcattgcc ctgcccggtg
    25681 cgtaatcagg tttctgtcga aaagccggac tggtcaacgg taccgtttat catggccaat
    25741 caggggccgg tgcgccgccg cattgagttg tggttccgac gcaataaaat cagtaacccg
    25801 atgatttacg ccacggttgg cgggcatgaa gcgatggtat cgatggtggc gctcggctgt
    25861 ggcgtggcat tgttgccgga agtggtgctg gaaaacagcc ccgaaccggt gcgtaaccgc
    25921 gtgatgattt tagagcgcag cgatgagaaa acgccgtttg agctgggcgt gtgtgcgcag
    25981 aaaaagcggc tacatgagcc gctaattgag gcattctgga agattttgcc gaaccattaa
    26041 cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagc
    26101 tcattcagcc gggtttcgaa atccggttca tggtcgccaa gttcgaacgc cgccagtacg
    26161 cgcccgtagt cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc
    26221 agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg
    26281 cgttcctgca acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc
    26341 atttcgtcgt cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt
    26401 tctttgcgct cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg
    26461 gcatcggcaa aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat
    26521 ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg
    26581 cccagttcgc agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa
    26641 agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc
    26701 gccagcgcca gcgcgccaga gggttccgcc accgcacgca catcttcgaa taaatccttc
    26761 atcgccgcac agatcgcatc gctatcgacg gtgatgatgt cgtcgagata ctcctggcat
    26821 aaacggaagg tttcgtcacc gatgcgtttt accgcgacac cttcggcgaa cagtcccacg
    26881 cgtggcagat caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc
    26941 gcttctacgg cgatcacttt gatttgcggc atcagttgtt tgatcagcac cgctacgccc
    27001 gccgccagac cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg
    27061 agcagttcca gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc
    27121 acccaggtga acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag
    27181 ttcgcgccat gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg
    27241 gcggtggcgg ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg
    27301 acgccctgcg cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc
    27361 gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga
    27421 tcttcgcgct tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt
    27481 agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg
    27541 gcaccttccg gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg
    27601 atttatcgcg caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcacaggg
    27661 caaaggaaac ctgacgttca cggtttttcg gcgtccaggc tttgtcaccc cgagcttcct
    27721 gcgcttcacg acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg
    27781 ggatgtcgat agcgatcagg tcaccatctt caatcagacc aatgctgccg ccgcttgccg
    27841 cttccggtga gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg
    27901 tgatcagcgc acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca
    27961 tttcctgcat gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg
    28021 cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg
    28081 ccgggccggt gaatttgagg atgctgtcat cgacgcccgc ggttttcacg atgcagccgt
    28141 tttcagcaaa attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca
    28201 gcgagcggat acagccgttt gcgcgatcgt catcgagaga atcccaacgg caatcctgcg
    28261 agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg gaacatattt tttaccgcgt
    28321 catcctgggt cagcataacg tcgtattgtt ccagagtttg cggcaacgtc aggccaagta
    28381 cgtttttcac atcacggttc agtaaccccg cgcgatccag ttcaccgaga ataccgataa
    28441 caccaccagc acggtgaaca tcttccatat ggtatttttg ggtgctcggc gcaactttgc
    28501 ataactgcgg gactttacgc gaaagtttgt cgatatcact catggtgaag tcgatttccg
    28561 cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt agagccgccc atcgcgatat
    28621 ccagcgtcat ggcattttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt
    28681 cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga
    28741 acaactgctt acggtccgcg tgggttgcca gcagcgagcc gttgcccggc tgcgacaggc
    28801 ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc
    28861 aggtcggaca cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg
    28921 cgccctggat catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt
    28981 tcccggcctc catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag
    29041 ccatcagcat ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc
    29101 agtgggcgtt gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggaagtgaat
    29161 aaagcatccc cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt
    29221 tggcaacgcc gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat
    29281 ggacgtgacc cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa
    29341 aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac
    29401 catgagtggt ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct
    29461 cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac
    29521 cagccccatt tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt
    29581 ttggtaaccg ggccacaacg gccttcgcca atctgaatac cgtctacgct gcgcactggc
    29641 gtgatttctg ccgccgtacc ggacataaag acttcatccg ccaagtacag ggattcgcgc
    29701 gacagcacct gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca
    29761 cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc
    29821 acttcaaaca ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata
    29881 ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta
    29941 ccaccggctt ttgccgcagt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc
    30001 atcgcatcga tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg
    30061 gcgataatca catcggttga gtatcccgct ggcgggttta ctcccatgcc aacatcaccg
    30121 acgaagatca gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga
    30181 caagcttcca tcagctcatc aatgctctgt gaaaccggga agcgatagat tttggcggag
    30241 tcatgcagac gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgtgagtcg
    30301 tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca gcgcgtgcga catcacatgc
    30361 accttcgcgt cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc
    30421 gtggtcattt ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct
    30481 ctggcagatg gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc
    30541 gaccgaccgt gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc
    30601 agccatattc attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc
    30661 taaggtttcc ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc
    30721 tccaacattt ctgaattact ggcacctggc ggcaccagcg gccagacgtt ctcaagttcg
    30781 tcgattgaga catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct
    30841 tcaacctggt ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg
    30901 aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc
    30961 tgccattgtc gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac
    31021 tgcttgcgtt ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag
    31081 atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc caactgccgc cggtaaacca
    31141 aaacccatgg tacctaagcc gctggaggtg atgaaatttt ccgggcgagt gtgggcgatg
    31201 tgctgcgcag cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt
    31261 ttacgatccg acagttgttt taacaacaac ggagcgtaga tagcgtcacc gggatggtcg
    31321 taacgccagg aatgttcatc acgcagctgc gcgcagtgtt gctgccagtc attgatattt
    31381 aacggctgct gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc
    31441 tgacgcagct tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggt
    31501 gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc
    31561 aggtcacact cctgcaccgc gaagtttgcc gctttggttc cgtgcatccc cagcatgccc
    31621 agatagtacg gataatctgc ttctactgcg cccagccctt tcagcgtaca ggtggcaggc
    31681 atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg cctgcgccat ccccacgcca
    31741 ccgccaacgt acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcgact
    31801 tcggcatgtg ggaaagtcac ttcgttttca acggtggtga accacggttc cagatcgccg
    31861 ctggctaact ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg
    31921 gcaacgtcga atgcttcagc catgatgcgc ggcaactctt ccagagactg caccagaaag
    31981 ctgtgcttgg tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtcggtg
    32041 ccgataaacg gtgcggacac ctgaccagtg atggcaacga cagggatgga atctaacagt
    32101 gcgtccgcaa gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg
    32161 ccagttttac cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt
    32221 cggcacagca agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg
    32281 ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac
    32341 tgtgcgccat tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt
    32401 tattcattgt ctgctcctcg gttatgtttt taaggtcaaa aaaacccccg gacctttcgg
    32461 tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct ttcctcgtcc aagtgcagcc
    32521 ccgcacggtg ggataataat caccaccacg ctaatcacga ccaggctaat cactcgtaga
    32581 agggctgtca ttttgtcttt tcttgcatct tgttcgaagg aatgcctaaa gagttaccat
    32641 agattttgcg aatagcacaa gatatttttg ggcctatttt tatgatggct ttaataattt
    32701 caatagaaat tgttgttttt taaaaagaaa atagtgagtg aaaaaattca atttcagtat
    32761 aaatgttgtt cccttcctcg tagttcatcc tttgtccgct gcatttttgc gagcatcatt
    32821 ccaccgccag aacaactcaa cttcacttgc acaaataaac aggaatcagc cactaaaatt
    32881 cacgaattag cgggctacag cagcgatatc ttatgcacat aatggtgact caaggagggt
    32941 ttatgtcact gtcaattgtt catacccgcg cagccctgga agtaaatgcg cccccaatca
    33001 ctgttgaggt acatatcagt aaaggtctac ccggcttaac gatggtgggc ttaacagaaa
    33061 caacggtgaa agaagctcgc gatcgcgtgc gcagcgccat tatcaatagc ggatatgaat
    33121 atccggcgaa aaaaatcacc atcaaccttg ctccagccga tctgccaaaa gaagggggac
    33181 gatatgattt acctatcgcc attgcgttgc tggcggcctc agaacagctt acagccaata
    33241 agttagatga atatgaatta gtcggagaac tagcgcttac aggcgctctg cgtggcgttc
    33301 ccggtgcaat ctccagtgca actgaagcta ttaagtcggg cagaaaaatt atcgtcgcga
    33361 aagataacga agatgaagtg gggctaatta acggtgaagg atgcctgata gccgatcatc
    33421 tgcaggctgt ctgtgcgttt ctggaaggta agaacactct cgaacgcccg aaaccaactg
    33481 atgcagtatc ccgggcgcta caacatgatc tcagtgatgt tgtcggtcag gagcaaggaa
    33541 aacgaggact ggaaattacc gccgctggtg ggcacaacct tttactgatt gggccgccgg
    33601 gaacaggtaa aacaatgctc gccagccgta ttaatggcct gttgccagat ttaagcaatg
    33661 aagaggcact ggagagcgct gcgatattaa gtctggtaaa tgctgaatca gtacaaaaac
    33721 aatggcggca gcgcccgttc cgctcacctc atcacagtgc gtcgttaact gcgatggtgg
    33781 gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc gcataacggc gtgctttttc
    33841 ttgatgagct acctgaattt gaacggcgta cactggatgc cttgcgagag ccgattgaat
    33901 ccgggcagat ccatctttca cgcacacgag caaaaataac ctatccagcc cgtttccagc
    33961 ttgttgcggc gatgaatccc agccctaccg gacattatca gggaaaccat aaccgctgca
    34021 cgccagaaca gacattacgt tatctcactg gagatcccat taccaccccc cggcattttg
    34081 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34141 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34201 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34261 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34321 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34381 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    34441 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    34501 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    34561 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacaaaga gtttctcttc
    34621 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    34681 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    34741 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    34801 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    34861 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    34921 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    34981 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35041 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35101 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35161 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35221 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35281 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35341 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    35401 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    35461 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    35521 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    35581 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    35641 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    35701 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    35761 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    35821 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    35881 gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa cggacgggac
    35941 tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctcca
    36001 ccgaattctt ttacaaccac cggttttacg accggctt
//
LOCUS       DAJVPT010000031        34561 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVPT010000031 DAJVPT010000000
VERSION     DAJVPT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1858
                     /locus_tag="OQM47_001498"
     CDS             <1..1858
                     /locus_tag="OQM47_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1479703.1"
                     /translation="PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYS
                     VALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLP
                     LHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANW
                     SNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQ
                     NNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRND
                     NGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYND
                     SFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYS
                     STLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQS
                     DSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNY
                     NYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLS
                     VVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1855..2943
                     /locus_tag="OQM47_001499"
     CDS             1855..2943
                     /locus_tag="OQM47_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1479704.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3031..4329)
                     /gene="exuT"
                     /locus_tag="OQM47_001500"
     CDS             complement(3031..4329)
                     /gene="exuT"
                     /locus_tag="OQM47_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1479705.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4814..6226
                     /gene="uxaC"
                     /locus_tag="OQM47_001501"
     CDS             4814..6226
                     /gene="uxaC"
                     /locus_tag="OQM47_001501"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1479706.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6241..7728
                     /gene="uxaA"
                     /locus_tag="OQM47_001502"
     CDS             6241..7728
                     /gene="uxaA"
                     /locus_tag="OQM47_001502"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1479707.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7811..8362
                     /locus_tag="OQM47_001503"
     CDS             7811..8362
                     /locus_tag="OQM47_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1479708.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8367..9611)
                     /gene="sstT"
                     /locus_tag="OQM47_001504"
     CDS             complement(8367..9611)
                     /gene="sstT"
                     /locus_tag="OQM47_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1479709.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10207..11172)
                     /gene="alx"
                     /locus_tag="OQM47_001505"
     CDS             complement(10207..11172)
                     /gene="alx"
                     /locus_tag="OQM47_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1479710.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11443..12441)
                     /locus_tag="OQM47_001506"
     CDS             complement(11443..12441)
                     /locus_tag="OQM47_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1479711.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12520..13212)
                     /locus_tag="OQM47_001507"
     CDS             complement(12520..13212)
                     /locus_tag="OQM47_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1479712.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13289..13792)
                     /locus_tag="OQM47_001508"
     CDS             complement(13289..13792)
                     /locus_tag="OQM47_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1479713.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13877..15013
                     /gene="rlmG"
                     /locus_tag="OQM47_001509"
     CDS             13877..15013
                     /gene="rlmG"
                     /locus_tag="OQM47_001509"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1479714.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15298..15612
                     /gene="higB"
                     /locus_tag="OQM47_001510"
     CDS             15298..15612
                     /gene="higB"
                     /locus_tag="OQM47_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1479715.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15609..16025
                     /gene="higA"
                     /locus_tag="OQM47_001511"
     CDS             15609..16025
                     /gene="higA"
                     /locus_tag="OQM47_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1479716.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16070..18088)
                     /gene="fadH"
                     /locus_tag="OQM47_001512"
     CDS             complement(16070..18088)
                     /gene="fadH"
                     /locus_tag="OQM47_001512"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1479717.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18314..20665)
                     /gene="ygjK"
                     /locus_tag="OQM47_001513"
     CDS             complement(18314..20665)
                     /gene="ygjK"
                     /locus_tag="OQM47_001513"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1479718.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20682..21101)
                     /locus_tag="OQM47_001514"
     CDS             complement(20682..21101)
                     /locus_tag="OQM47_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479719.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21079..21432)
                     /locus_tag="OQM47_001515"
     CDS             complement(21079..21432)
                     /locus_tag="OQM47_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479720.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21433..21753)
                     /locus_tag="OQM47_001516"
     CDS             complement(21433..21753)
                     /locus_tag="OQM47_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479721.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(21887..23320)
                     /locus_tag="OQM47_001517"
     CDS             complement(21887..23320)
                     /locus_tag="OQM47_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1479722.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23383..23832)
                     /locus_tag="OQM47_001518"
     CDS             complement(23383..23832)
                     /locus_tag="OQM47_001518"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1479723.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23829..26915)
                     /gene="ebgA"
                     /locus_tag="OQM47_001519"
     CDS             complement(23829..26915)
                     /gene="ebgA"
                     /locus_tag="OQM47_001519"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1479724.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27098..28081)
                     /gene="ebgR"
                     /locus_tag="OQM47_001520"
     CDS             complement(27098..28081)
                     /gene="ebgR"
                     /locus_tag="OQM47_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1479725.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28299..28630
                     /locus_tag="OQM47_001521"
                     /pseudo
     CDS             28299..28630
                     /locus_tag="OQM47_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28672..30051)
                     /gene="ygjG"
                     /locus_tag="OQM47_001522"
     CDS             complement(28672..30051)
                     /gene="ygjG"
                     /locus_tag="OQM47_001522"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1479726.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30469..31989
                     /gene="aer"
                     /locus_tag="OQM47_001523"
                     /pseudo
     CDS             30469..31989
                     /gene="aer"
                     /locus_tag="OQM47_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32143..32766)
                     /gene="nfeR"
                     /locus_tag="OQM47_001524"
                     /gene_synonym="yqjI"
     CDS             complement(32143..32766)
                     /gene="nfeR"
                     /locus_tag="OQM47_001524"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1479727.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33043..33788
                     /gene="nfeF"
                     /locus_tag="OQM47_001525"
                     /pseudo
     CDS             33043..33788
                     /gene="nfeF"
                     /locus_tag="OQM47_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33842..33917)
                     /locus_tag="OQM47_001526"
     tRNA            complement(33842..33917)
                     /locus_tag="OQM47_001526"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33882..33884),aa:Ile,seq:cat)
     gene            34042..34548
                     /gene="mug"
                     /locus_tag="OQM47_001527"
                     /pseudo
     CDS             34042..34548
                     /gene="mug"
                     /locus_tag="OQM47_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8425 a         8896 c         9087 g         8153 t
ORIGIN      
        1 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
       61 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      121 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      181 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      241 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      301 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      361 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      421 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      481 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      541 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      601 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      661 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      721 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
      781 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
      841 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
      901 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
      961 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1021 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1081 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1141 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1201 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1261 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1321 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1381 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1441 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1501 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1561 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1621 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1681 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1741 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     1801 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     1861 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     1921 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     1981 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2041 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2101 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2161 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2221 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2281 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2341 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2401 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2461 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2521 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2581 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2641 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2701 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     2761 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     2821 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     2881 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     2941 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3001 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3061 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3121 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3181 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3241 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3301 agacagtgct tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3361 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3421 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3481 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3541 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3601 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3661 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3721 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     3781 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     3841 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     3901 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     3961 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4021 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4081 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4141 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4201 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4261 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4321 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4381 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4441 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4501 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4561 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4621 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4681 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4741 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     4801 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     4861 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     4921 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     4981 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5041 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5101 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5161 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5221 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5281 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5341 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5401 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5461 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5521 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5581 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5641 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5701 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     5761 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     5821 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     5881 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     5941 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6001 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6061 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6121 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6181 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6241 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6301 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6361 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6421 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6481 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6541 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6601 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6661 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6721 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     6781 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     6841 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     6901 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     6961 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7021 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7081 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7141 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7201 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7261 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7321 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7381 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7441 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7501 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7561 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7621 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7681 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7741 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     7801 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     7861 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     7921 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     7981 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8041 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8101 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8161 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8221 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8281 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8341 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8401 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8461 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8521 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8581 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8641 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8701 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     8761 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     8821 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     8881 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     8941 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9001 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9061 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9121 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9181 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9241 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9301 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9361 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9421 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9481 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9541 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9601 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9661 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9721 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
     9781 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
     9841 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
     9901 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
     9961 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10021 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10081 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10141 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10201 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10261 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10321 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10381 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10441 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10501 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10561 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10621 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10681 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10741 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    10801 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    10861 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    10921 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    10981 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11041 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11101 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11161 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11221 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11281 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11341 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11401 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11461 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11521 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11581 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11641 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11701 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    11761 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    11821 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    11881 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    11941 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12001 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12061 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12121 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12181 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12241 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12301 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12361 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12421 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12481 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12541 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12601 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12661 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12721 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    12781 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    12841 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    12901 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    12961 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13021 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13081 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13141 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13201 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13261 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13321 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13381 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13441 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13501 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13561 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13621 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13681 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13741 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    13801 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    13861 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    13921 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    13981 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14041 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14101 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14161 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14221 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14281 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14341 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14401 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14461 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14521 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14581 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14641 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14701 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    14761 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    14821 cgataacgtt gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    14881 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    14941 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15001 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15061 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15121 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15181 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15241 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15301 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15361 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15421 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15481 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15541 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15601 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15661 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15721 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    15781 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    15841 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    15901 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    15961 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16021 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16081 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16141 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16201 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16261 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16321 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16381 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16441 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16501 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16561 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16621 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16681 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16741 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    16801 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    16861 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    16921 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    16981 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17041 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17101 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17161 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17221 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17281 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17341 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17401 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17461 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17521 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17581 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17641 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17701 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    17761 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    17821 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    17881 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    17941 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18001 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18061 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18121 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18181 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18241 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18301 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18361 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18421 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18481 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18541 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18601 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18661 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18721 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    18781 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    18841 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    18901 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    18961 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19021 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19081 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19141 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19201 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19261 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19321 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19381 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19441 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19501 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19561 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19621 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19681 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19741 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    19801 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    19861 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    19921 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    19981 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20041 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20101 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20161 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20221 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20281 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20341 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20401 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20461 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20521 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20581 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20641 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20701 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    20761 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    20821 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    20881 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    20941 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21001 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21061 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21121 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21181 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21241 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21301 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21361 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21421 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21481 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21541 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21601 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21661 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21721 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    21781 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    21841 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    21901 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    21961 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22021 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22081 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22141 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22201 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22261 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22321 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22381 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22441 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22501 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22561 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22621 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22681 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22741 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    22801 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    22861 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    22921 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    22981 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23041 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23101 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23161 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23221 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23281 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23341 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23401 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23461 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23521 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23581 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23641 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23701 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    23761 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    23821 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    23881 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    23941 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24001 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24061 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24121 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24181 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24241 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24301 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24361 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24421 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24481 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24541 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24601 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24661 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24721 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    24781 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    24841 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    24901 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    24961 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25021 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25081 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25141 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25201 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25261 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25321 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25381 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25441 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25501 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25561 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25621 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25681 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25741 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    25801 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    25861 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    25921 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    25981 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26041 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26101 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26161 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26221 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26281 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26341 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26401 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26461 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26521 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26581 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26641 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26701 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    26761 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    26821 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    26881 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    26941 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27001 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27061 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27121 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27181 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27241 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27301 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27361 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27421 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27481 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27541 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27601 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27661 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27721 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    27781 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    27841 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    27901 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    27961 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28021 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28081 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28141 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28201 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28261 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28321 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28381 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28441 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28501 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28561 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28621 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28681 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28741 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    28801 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    28861 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    28921 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    28981 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29041 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29101 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29161 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29221 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29281 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29341 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29401 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29461 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29521 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29581 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29641 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29701 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    29761 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    29821 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    29881 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    29941 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30001 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30061 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30121 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30181 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30241 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30301 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30361 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30421 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30481 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30541 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30601 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30661 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30721 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    30781 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    30841 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    30901 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    30961 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31021 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31081 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31141 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31201 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31261 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31321 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31381 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31441 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31501 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31561 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31621 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31681 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31741 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    31801 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    31861 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    31921 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    31981 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32041 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32101 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32161 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32221 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32281 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32341 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32401 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32461 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32521 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32581 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32641 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32701 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    32761 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    32821 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    32881 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    32941 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33001 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33061 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33121 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33181 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33241 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33301 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33361 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33421 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33481 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33541 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33601 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33661 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33721 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    33781 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    33841 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    33901 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    33961 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34021 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34081 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34141 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34201 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34261 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34321 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34381 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34441 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34501 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
    34561 g
//
LOCUS       DAJVPT010000032        34134 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVPT010000032 DAJVPT010000000
VERSION     DAJVPT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..365)
                     /locus_tag="OQM47_001528"
     CDS             complement(<1..365)
                     /locus_tag="OQM47_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1479728.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRL"
     gene            650..1871
                     /gene="mdfA"
                     /locus_tag="OQM47_001529"
                     /pseudo
     CDS             650..1871
                     /gene="mdfA"
                     /locus_tag="OQM47_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(1912..2196)
                     /locus_tag="OQM47_001530"
     CDS             complement(1912..2196)
                     /locus_tag="OQM47_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479729.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(2282..3097)
                     /gene="ybjI"
                     /locus_tag="OQM47_001531"
     CDS             complement(2282..3097)
                     /gene="ybjI"
                     /locus_tag="OQM47_001531"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1479730.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(3097..4305)
                     /locus_tag="OQM47_001532"
     CDS             complement(3097..4305)
                     /locus_tag="OQM47_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1479731.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4389..4925
                     /gene="rcdA"
                     /locus_tag="OQM47_001533"
     CDS             4389..4925
                     /gene="rcdA"
                     /locus_tag="OQM47_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1479732.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(5100..6785)
                     /locus_tag="OQM47_001534"
     CDS             complement(5100..6785)
                     /locus_tag="OQM47_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1479733.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7055..7432
                     /locus_tag="OQM47_001535"
     CDS             7055..7432
                     /locus_tag="OQM47_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1479734.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(7462..7719)
                     /gene="grxA"
                     /locus_tag="OQM47_001536"
     CDS             complement(7462..7719)
                     /gene="grxA"
                     /locus_tag="OQM47_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1479735.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            7879..8166
                     /locus_tag="OQM47_001537"
     CDS             7879..8166
                     /locus_tag="OQM47_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1479736.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            8150..8872
                     /gene="nfsA"
                     /locus_tag="OQM47_001538"
     CDS             8150..8872
                     /gene="nfsA"
                     /locus_tag="OQM47_001538"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1479737.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            8933..9835
                     /gene="rimK"
                     /locus_tag="OQM47_001539"
     CDS             8933..9835
                     /gene="rimK"
                     /locus_tag="OQM47_001539"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1479738.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            9923..10399
                     /locus_tag="OQM47_001540"
     CDS             9923..10399
                     /locus_tag="OQM47_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1479739.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            10751..11863
                     /gene="potF"
                     /locus_tag="OQM47_001541"
     CDS             10751..11863
                     /gene="potF"
                     /locus_tag="OQM47_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1479740.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            11958..13091
                     /gene="potG"
                     /locus_tag="OQM47_001542"
     CDS             11958..13091
                     /gene="potG"
                     /locus_tag="OQM47_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1479741.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            13101..14054
                     /gene="potH"
                     /locus_tag="OQM47_001543"
     CDS             13101..14054
                     /gene="potH"
                     /locus_tag="OQM47_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1479742.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14051..14896
                     /gene="potI"
                     /locus_tag="OQM47_001544"
     CDS             14051..14896
                     /gene="potI"
                     /locus_tag="OQM47_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1479743.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            14956..15444
                     /locus_tag="OQM47_001545"
     CDS             14956..15444
                     /locus_tag="OQM47_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1479744.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            15485..16612
                     /gene="rlmC"
                     /locus_tag="OQM47_001546"
     CDS             15485..16612
                     /gene="rlmC"
                     /locus_tag="OQM47_001546"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1479745.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16787..17518)
                     /gene="artJ"
                     /locus_tag="OQM47_001547"
     CDS             complement(16787..17518)
                     /gene="artJ"
                     /locus_tag="OQM47_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1479746.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17810..18478)
                     /gene="artM"
                     /locus_tag="OQM47_001548"
     CDS             complement(17810..18478)
                     /gene="artM"
                     /locus_tag="OQM47_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1479747.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(18478..19194)
                     /gene="artQ"
                     /locus_tag="OQM47_001549"
     CDS             complement(18478..19194)
                     /gene="artQ"
                     /locus_tag="OQM47_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1479748.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(19201..19932)
                     /gene="artJ"
                     /locus_tag="OQM47_001550"
     CDS             complement(19201..19932)
                     /gene="artJ"
                     /locus_tag="OQM47_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1479749.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(19950..20678)
                     /gene="artP"
                     /locus_tag="OQM47_001551"
     CDS             complement(19950..20678)
                     /gene="artP"
                     /locus_tag="OQM47_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1479750.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(20896..21411)
                     /locus_tag="OQM47_001552"
     CDS             complement(20896..21411)
                     /locus_tag="OQM47_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1479751.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22060..23829
                     /locus_tag="OQM47_001553"
     CDS             22060..23829
                     /locus_tag="OQM47_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1479752.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            24040..24363
                     /locus_tag="OQM47_001554"
     CDS             24040..24363
                     /locus_tag="OQM47_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1479753.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            24360..25190
                     /gene="amiD"
                     /locus_tag="OQM47_001555"
     CDS             24360..25190
                     /gene="amiD"
                     /locus_tag="OQM47_001555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1479754.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(25187..26200)
                     /locus_tag="OQM47_001556"
     CDS             complement(25187..26200)
                     /locus_tag="OQM47_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1479755.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(26299..27729)
                     /locus_tag="OQM47_001557"
     CDS             complement(26299..27729)
                     /locus_tag="OQM47_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1479756.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(27740..28741)
                     /gene="ltaE"
                     /locus_tag="OQM47_001558"
     CDS             complement(27740..28741)
                     /gene="ltaE"
                     /locus_tag="OQM47_001558"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1479757.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(28778..30496)
                     /gene="poxB"
                     /locus_tag="OQM47_001559"
     CDS             complement(28778..30496)
                     /gene="poxB"
                     /locus_tag="OQM47_001559"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1479758.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(30629..31597)
                     /gene="hcr"
                     /locus_tag="OQM47_001560"
     CDS             complement(30629..31597)
                     /gene="hcr"
                     /locus_tag="OQM47_001560"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1479759.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(31609..33261)
                     /gene="hcp"
                     /locus_tag="OQM47_001561"
                     /gene_synonym="priS"
     CDS             complement(31609..33261)
                     /gene="hcp"
                     /locus_tag="OQM47_001561"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1479760.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(33405..>34134)
                     /gene="lysO"
                     /locus_tag="OQM47_001562"
     CDS             complement(33405..>34134)
                     /gene="lysO"
                     /locus_tag="OQM47_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1479761.1"
                     /translation="LLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSR
                     IAMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLK
                     QIVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESF
                     GPVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDM
                     VPAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8452 a         8441 c         8788 g         8453 t
ORIGIN      
        1 aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc gtgatcgctc
       61 gggaatgagt catccgccgc atgatgcagg aagttatagc cgatattttc gacaaagggt
      121 cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa acaggctgac cgccagcgcg
      181 atagcgattt ttatcaccag ttgccgttgt gctgtaagcc cccacaacca aagtaccgcg
      241 gccagcaacg gcaccacggt aatcaaatct ttagcaataa aaatcgccaa cgagatcatc
      301 cacggagccg agtctggcgt cgcgttaata agagagaata gagagagatt caaattttcc
      361 agcataactt cccgacgcaa agtgattaaa agggagccaa tacaggcaag tcgttgagaa
      421 taaagtgcag gttaaactgg gtaaagcggc atcgtcttat ttccctcaag cggcctgttt
      481 acggtgggtg attgtaacgg gcataggtta aataaaactt aaagaaagcg tagctatact
      541 cgcaataatg taagaatgtg cttaaccgtg gtttcagcta caaaattcgc tttctcgtta
      601 gctgcgcttt tattaaactc tgcgcgatta ttattggcga agaaattgca tgcaaaataa
      661 attagcttcc ggtgccaggc ttggacgtca ggcgttactt ttccctctct gtctggtgct
      721 ttacgaattt tcaacctata tcggcaacga tatgatccaa cccggtatgt tggccgtggt
      781 ggaacaatat caggcgggca ttgattgggt tcctacttcg atgaccgcgt atctggcggg
      841 cgggatgttt ttacaatggc tcctggggcc gctgtcggat cgtattggtc gccgtccggt
      901 gatgctggcg ggtgtggttt atcgtcacct gtctggcaat attactggcg caaaacattg
      961 aacaattcac cctgttgcgc ttcttgcagg gcataagctt ctgtttcatt ggcgctgtgg
     1021 gatacgccgc aattcaggaa tccttcgaag aggcggtttg tatcaagatc accgcgctga
     1081 tggcgaacgt ggcgctgatt gctccgctac ttggtccgct ggtgggcgcg gcgtggatcc
     1141 atgtgctgcc ttgggagggg atgtttgttt tgtttgccgc attggcagcg atctcctttt
     1201 tcggtctgca acgagctatg cctgaaaccg ccatgcgtat aggcgagaaa ctgtcactga
     1261 aagaactcgg tcgtgactat aagctggtgc tgaagaacgg ccgctttgtg gcgggggcgc
     1321 tgggattcgt tagcctgcca ttgctggcgt ggatcgccca gtcgccgatt atcatcatta
     1381 ccggggagca gttgagcagc tatgaatatg gcttgctgca agtgcctatt ttcggggcgt
     1441 taattgcggg taacttgctg ttagcgcgtc tgacctcgcg ccgcaccgta cgttcgctga
     1501 ttattatggg cggctggccg attatgattg gtctgttggt cgctgctgcg gcaacggtta
     1561 tctcatcgca cgcgtattta tggatgaccg ccgggttaag tatttatgct ttcggtattg
     1621 gtctggcgaa tgcgggactg gtgcgattaa ccctgtttgc cagcgatatg agtaaaggta
     1681 cggtttctgc ggcgatggga atgctgcaaa tgctgatctt taccgtcggt attgaaatca
     1741 gcaaacatgc ctggctgaac gggggcaacg gactgtttaa tctcttcaac cttgtcaacg
     1801 gaattttgtg gctgtcgctg atggttatct ttttaaaaga taaacagatg ggaaattctc
     1861 acgaagggta aaaaaatgcc tgattgattt gtgcgatcag gcattctcga attaatggtg
     1921 atggtcgtca atctggtgtt cgataaccat cccttcacct acgctggcaa gatggcgaac
     1981 ataaggatgc gggcggtaag ccggagctgg cgcaggagcc acatagacgg tttgcggcac
     2041 agtcgcgacg ctgaccgctt gtggaacgct gactgaatca ggaaccacaa ccactttgta
     2101 tccactcggc acatcgacgg tgacactttg cgccatcgcc acaccagtaa agcccattaa
     2161 aagcgcgccc aacagtagac aatttttcat aatcatttcg cctgaaataa tgcagcgggt
     2221 taatttccgc gaattatgca gccttcattc aggcattgtg ttgccgggcg tgctgccgtt
     2281 tttattggtc aaatggcgct tcgtgtttaa gaactttatc gatcacatcc agtacgcctt
     2341 cacggttatt gtagcctgcc cggtattttg ccgctgcgac gactgcgctg ccggcatttt
     2401 ccattgcaaa actaaagcct gcctgacgca gcatctcaat atcgttaccg ccatcgccaa
     2461 agaccaccac ttcgctgtcg tctattcccc ataatttctg cagttggcga aggccattgg
     2521 ctttatgtac gccggggata atcagatcga tgctgccgtt gccggtgtgg actggcacca
     2581 taatatcgcc gatggcctca tgtaatgctt tttgtacttg tggaatcagt tcatcggaaa
     2641 gattcaggcc aaacttaaag aaaatatctt ctaagttgtc aaagttatcg acgtattcca
     2701 gacggtgata atacatttcc gccaccgttt tcatggcatc gtcatatttt ttgagtgtat
     2761 aggcgctatt ttttccgcag gcaataattt ccacttccgg gcgcgtcagc aaatgttcca
     2821 cgacagtagc aaacgcatcc ttcgacagct cgccattaaa aacatctttg ccttcactca
     2881 ttacccagcc gccgttttca gccacaaagg caatttcatt cgcaatttca gggaagaaag
     2941 agatcaactg ataatattga ttcccgctgg cgaccacaaa gcgaatgcct tgtgctttca
     3001 tttgctgata ctgagccata aaccgctcac ggttataggt tttttgatcg cttaagaaag
     3061 taccatccat gtctaccgca attaatttaa tgctcatcaa ctattctcca tcgccgtctg
     3121 cgttttggta tcgggtttgg cgacggcttt cgcgacaata gccgcgagaa taaccagcgc
     3181 cagtacaacc agcattgcac tacgtaatcc ataatgttcg ccgagatagc ccagcagcgg
     3241 cggcccgacg aggaaagcca gataaccggt cgttgctacc acactgacgc gggttggtgc
     3301 atcggggccg gtatcgctgg cggcagaaat ggtcagcggg aagcccagcg aggcacccag
     3361 tccccaaagt acaacagaca ctcctgcgac ccaggcgcta tcgacaaaaa taatcagccc
     3421 aatacccaac gcccccatta gtgcactggc ccgaaccacg gcaacgcgac tgtaactgtc
     3481 gatgaaccaa ccgccggtga agcgtccaac ggtcatcccc agggtaaaac cggcataaat
     3541 cagtgagccg gaagtggggc taaaaccgtg accatcaacc attaataagg gtaaccagtc
     3601 gttggcagaa ccttcggcaa aggccatcgc cagcactaca acaccaatca acagcaactg
     3661 gatatcgcga taaaaaggaa tgcctttttc gccatgctgg gtgccatcgg cagcattttt
     3721 gcccgtaccg tcagggattg cctgaatggc gatataaata ggcgcgatac ctaccagcgc
     3781 cgccaataaa atgtgcaccg ttgccggaac gccaaaggcc gtcagtgcca tcccgacacc
     3841 agcgcctgcc agcgtgccca ggctataaaa accgtgcatc atcggcaaaa ccgttttatt
     3901 catttctcgc tcaacggcgg caccttcaac gtttatcgcc acttccgcag aaccaaaact
     3961 tgccccaaag acgccgagac caacggcaaa gagcaggggc gatgtcagcc agagtgccag
     4021 acttaatatc atcatcccga tcaatgcgca ggacatcgtg accaggatga cattacgtgt
     4081 cccaaagcgt ttcactaacc acgccgagca gagaataccg ctcatcgaac cgatcgacag
     4141 accaaagaga acaccgccca tttcagcgat cgagacagag agaatatcgc ggatagcagg
     4201 cgtacgggtt gcccaggacg ccattaacag gcctggcaaa aagaagaaca taaacagcgc
     4261 ccaggttcgg cgtttcaatg cattacgtga agaatttacg gtcatagatc acgtcaaaat
     4321 aagaagaggg aagacaacat tagcaaggtt gtgtacattt gtacacaatt gcagacagag
     4381 gaaatgacat gcgtcgcgct aacgatccgc aacggcgaga aaaaattatc caggccacgc
     4441 tggaggcggt gaaactttac ggaatacatg ctgttacgca ccgcaaaatt gctacccttg
     4501 ccggggtacc gttgggatcg atgacctact atttttcagg aattgatgag ttgttgctgg
     4561 aggcgttcag cagttttact gagattatgt cccggcaata tcaggcattt tttagcgatg
     4621 ttagtgatgc tcaaggcgca tgccaggcta tcaccgatat gatctacagc tcacaggttg
     4681 caacgccgga taatatggag ctgatgtacc agctctacgc gctggctagc cgaaaaccgc
     4741 tattaaaaac ggtaatgcaa aactggatgc agcgcagtca gcaaacgctc gaacaatggt
     4801 ttgaacccgg aaccgcccgc gcgcttgatg cgtttattga ggggatgacg ctgcattttg
     4861 tcaccgaccg taagccgcta tcgcgcgagg agattttgag gatggttgag agggttgcag
     4921 ggtagtagat aaattttaga taacaaaaaa cccatcaacc ttgaaccgaa atggcggggt
     4981 tgatgggctc cacaaaatgg ggacatcaaa gaaaagcagt ggcactaatt aagactgatg
     5041 ccctgcggaa aagttctgcg gttgtgcaaa aaaatttcat tttcagggca acttcagttt
     5101 tatcctaatc ctggccatac catgacgatg attgtccctg ccagcgtcag caggacgttg
     5161 gcgattgcgt aggtgcccgc atagcccagc gcagggatgt tactgcgagc tgtatcactg
     5221 atgatctcca ttgccggcgc gcaggtgcgt gcgcccatca ttgcgccgaa caacagtgcg
     5281 cggttcattc gcaatacata agcaccgaac aagaaacaga taaccacggg cacaagactg
     5341 acaattaatc cggcaatcaa catctgaccg ccaatcgcgc ccaggccgtt attaataccg
     5401 ctaccggcgc tcagaccaac gcctgccata aacaccatca agccgaactc tttcaccatg
     5461 cttaatgcac cttgcggaat gtaaccgaag gtcgggtggt tagcacgcat aaagcccagc
     5521 ataattccgg cgaataacaa cccggcagcg ttccccatgc cgaaactgaa tgtgctgaac
     5581 tggaaggtga tcatcccgat catcagccca ataacaaaga aggcgcaaaa tgccagcagg
     5641 tcagtgacct ggctgtgaat cgagataaag ccgatgcgat cggcgatggt ttttacgcga
     5701 cgggcatcac cgctgacttg taaaacgtca cctttgttaa gcacgacgtt gtcatctatc
     5761 ggcatctcaa tctggctacg aatgacgcgg ttaaggaagc aaccgtgatc ggtcaacttc
     5821 agttgtgcga gacgtttacc tacagcgtta tggtttttaa cgaccacttc ttcagtgacg
     5881 atacgcatgt cgagaaggtc acgatcgaaa acttctttac cgttacggaa gctgggatcg
     5941 agtcgggcat gggcgtcggg atagcctacc aacgctattt catcgcccat ttgtagcacg
     6001 gcatcaccgt ctggatttgc cagaatcccg ttacgtcgaa tacgttcaat gtagcagccg
     6061 gtttgtcgat aaatacccag ttcacgcaga tttttgccgt cggtccaggc caccagctcc
     6121 gggccgacgc gataggcgcg gatcaccggt aaataaacct tacggttggc atcagtgtcc
     6181 aggccacgtt cgcgggcgat ttgctgggcg ctggtctgta agtcctgatg ctgcaatttc
     6241 ggcaagtaac gcgcaccaac aatcaaactc accagaccga ttaaataggt taaggcatac
     6301 ccgaggctca gattatccag tgccagtgag agctgcctgc tttccatgcc ggaatgatgc
     6361 agtgtatcgc cagcaccgac cagaaccggt gtcgacgtca tagagcctgc taacataccg
     6421 gccgtcaggc caatatccca gccaaacagc ttacctaacc ctaaggcgat caccagcgca
     6481 ctgccaacca tcaccagtgc taacattagg taatttttcc catcgcgaaa aaaaatggaa
     6541 aaaaagttcg gtccggcttc gaccccgacg cagaaaataa acagcataaa gccaagatta
     6601 agcgcatcgg tgttaatgct gaaatgttgt tggcctaata acagcgatac gactaaaacg
     6661 ccaatggaat tacccagttg gatcgaacca agtcgtaact ttccgagaca tagcccaagc
     6721 gcgaggacca caaataataa cagaatgtaa ttcccattta acaattcggc gacgtttata
     6781 ttcacggagg ctaacttctt gtttactagt aagctgttga aagaaatgct aatttacgat
     6841 aatgtgtttt accagaattc agggcgcaga ttcattcagc gcacctaaac gatagtaaag
     6901 taacaatata ttttactagt gtaatcacat taggtatcaa cggctatatg aattgcgttg
     6961 gcctatatta gcatggaatg cgaagcggct ttatcttact gaacgccaca ctggcgaaaa
     7021 atgtgttcga tagacgcagt gtcaggagga acgagtgaaa cataaacaac gttgggcggg
     7081 ggcaatctgc tgttttgtcc tcttcattgt ggtgtgcctt tttctggcga cgcacatgaa
     7141 aggcgctttt cgggctgccg ggcatcctga aatcggcttg ctgtttttca ttcttcctgg
     7201 agcagttgcc agtttctttt cacagcgtag agaagtcctg aaacctctat ttggcgcaat
     7261 gctggcggca ccctgttcaa tgctcattat gcggctgttt ttttcaccga cgcgctcatt
     7321 ctggcaagag ctggcatggt tactaagcgc ggtgttctgg tgtgcgctgg gggcactgtg
     7381 tttcttattt atcagtagtt tgtttaaacc acagcacaga aaaaatcagt aaagccctca
     7441 acgcgagggc ttgtcagacg atcaggcgtc cagattttct ttcacccatg cagcaaaatc
     7501 ggtatagccg ccgatatgtt gctgatcgac aaaaatctgc ggcacggttt ctacgggttt
     7561 acctgccttt tgttgtagat cttctttagt gatcccttcc gcacgaatat ctacatactg
     7621 atactgaaaa tcatcgcgtt cattgctcaa tttctcagcc agatcttttg cacgcacaca
     7681 gtaagggcaa cccgaacgac caaaaataac ggtttgcatt atttctctcc tcatagattt
     7741 atgcctgtaa tgatcacgct aaaatgtatt cgctgaaagt aggtttaacc tgttgcatta
     7801 attgctaaaa gctataactg ttaaacacaa tacagtgaaa agttttagac tgaaggctca
     7861 ctttgcagag ggaagcgtat gcgcgcgatc ggtaaattgc ctaaaggcgt gttgatactg
     7921 gaatttatcg gaatgatgct actggcggtg gcgctgctgt cggtaagcga ctccctgtcg
     7981 ctgcctgagc cattttctcg gccagaagtg cagattctga tgatttttct cggtgttttg
     8041 ctcatgcttc ccgctgcggt ggtggttatt cttcaggtgg caaaacgtct tgccccacag
     8101 ctgatgaact gtccaccgca atattcacgt tcagaaagag aaaaagataa tgacgccaac
     8161 cattgaactt atttgtggcc atcgctccat tcgccatttc actgatgaac ccatttccga
     8221 agcgcagtgt gaggcgatta ttaacagcgc ccgtgcgacg tccagttcca gttttttgca
     8281 gtgtagtagc attattcgca ttaccgacaa agcattacgt gaagaactgg tgacgctgac
     8341 cggcgggcaa aaacacgtag cgcaagcggc ggagttctgg gtgttctgtg ccgactttaa
     8401 ccgccattta cagatctgtc cggatgctca gctcggcctg gcggaacaac tgttgctcgg
     8461 tgtcgttgat acggcaatga tggcgcagaa tgcattaacc gcagcggaat cgctgggatt
     8521 gggcggggta tatatcggcg gcctgcgcaa taatattgaa gcggtgacga aactgctgaa
     8581 attaccgcag catgttctgc cgctgtttgg gctgtgcctt ggctggcctg cggataatcc
     8641 ggatctcaag ccgcgtttac cggcctccat tttggtgcat gaaaacagct atcaaccgct
     8701 ggataaagac gcactggcgc agtatgacga gcaactggcg gaatattacc tcacccgtgg
     8761 cagcaataat cgctgggata cctggagcga tcatatccgc cgaacaatca ttaaagaaag
     8821 ccgcccattt attctggatt atttgcacaa acagggttgg gcgacgcgct aaaaccgcca
     8881 cgtcgatgta tgatacgcgg gcttttgacc aggtctgaca gagaggtgca gggtgaaaat
     8941 tgccatattg tcccgggatg gaacgctcta ttcgtgtaag cggctgcgtg aagccgctat
     9001 acagcgcggt cacctggttg aaattcttga tccgctttct tgctacatga acataaatcc
     9061 tgcggcgtct tctatttact acaaaggccg caagttaccc cattttgacg cagtgatccc
     9121 gcgtattggc accgccatta ccttttatgg gacggcggca ctgcgccagt tcgagatgct
     9181 ggggagctat ccgctcaatg agtcggtcgc cattgcccgg gcgcgtgaca aattgcgttc
     9241 catgcaactg ctggcgcgtc agggcatcga cctgcctgtc acgggcattg cgcattcgcc
     9301 ggatgatacc agcgatttaa tcgacatggt cggtggtgcg ccgctggtgg tcaagttggt
     9361 tgaaggcacg cagggaattg gcgtcgtgct ggcggagact cgtcaggcgg cggaaagcgt
     9421 gattgacgct ttccgcggtc tgaacgcgca tattctggtg caggaatata tcaaagaggc
     9481 gcaagggtgc gatatccgct gtctggttgt tggtgatgaa gtggtcgctg cgattgaacg
     9541 gcgggcgaaa gagggctatt ttcgttccaa tttgcatcgt ggcggcgcgg caagcgtcgc
     9601 cagtatcaca ccacaggagc gtgaaatcgc gataaaagcc gcgcgaacga tggcgctgga
     9661 cgttgctggt gtggatattc tgcgtgctaa tcgcgggccg ttggtgatgg aagtgaatgc
     9721 gtcgccgggg ctggaaggaa tagaaaaaac caccggtatc gatatcgcgg gtaaaatgat
     9781 ccgctggatc gaacgccacg ctacgacaga atattgcctg aaaacgggtg gttagtcgca
     9841 atcacattac tgatcatggt tttgcctgcg ctttttgcgt aagctgtgcc ggtcttttta
     9901 tcgaaagagg ttgtacaaaa ttatgacatc gctggtcgtt cctggtctgg atacgctgcg
     9961 tcaatggctc gatgacctgg ggatgagttt ttttgaatgt gataactgtc aggctctgca
    10021 tctgccccat atgcagaatt tcgacggtgt ctttgatgcc aaaatcgatc tgatcgataa
    10081 cacgatcctg ttttctgcca tggcggaagt ccgaccttca gccgtattgc cgctggcggc
    10141 ggatttatct gccatcaatg ccagttcgct gaccgtgaaa gcatttcttg atatgcagga
    10201 tgataatctg ccaaagctgg tggtttgcca gtctttatcc gttatgcagg gcgtaaccta
    10261 tgagcagttt gcatggttcg tgcgtcagag cgaagagcag atttcgatgg tcattcttga
    10321 agctaatgcc catcaactgc tgttaccgac tgacgatgaa gggcaaaaca acgttaccga
    10381 aaactatttt ctccactgat aattcctttc gagcacgcag tcgctgccgc agtgactgcg
    10441 cgctgcaaaa ttatctgctg tttttaacct tttcttaaag attatttcac ttctcttgtg
    10501 tcgatttggc tttatcacat agagcaaata cgcataaaaa tttgttaaat accgtttttt
    10561 aatccgagct atagtctcaa accctggcta aagttattct tgcgatgctt ctatatagcg
    10621 agcagtgctg gccgggagag agttctcttt tcttacaccg cgccgataaa aaaatatgca
    10681 cgtttattgc atatctttca gtgtgacaac ttttgttcgt ttgttaacga actttcagaa
    10741 ggaaagagat gtgaccgcct taaataaaaa atggctatcg ggtctggttg cgggtgctct
    10801 gatggccgtc tctgtcggca cgctcgcggc tgaacaaaaa acactccaca tttataactg
    10861 gtctgattat atcgcccctg acacggtggc caattttgaa aaagaaaccg gtattaaagt
    10921 cgtctacgat gttttcgact ctaacgaagt actggaaggc aaattaatgg cgggtagcac
    10981 cggctttgac ttggtggttc catctgccag ctttctagaa cgccagttga ctgcgggagt
    11041 tttccagccg ctggacaaaa gcaaattgcc ggagtggaag aatctcgacc cggagctgct
    11101 gaagctggtc gccaaacacg atcccgacaa taaatttgct atgccctata tgtgggcgac
    11161 gaccgggatt ggctataacg ttgataaagt taaagctgtg ctgggcgaaa acgcgcccgt
    11221 cgatagctgg gacttgatcc tcaaacctga aaatctggaa aaactgaaaa gctgcggtgt
    11281 ctctttcctg gatgcgccag aagaagtttt tgctaccgtg ttgaattatc tcggcaaaga
    11341 tcccaacagc actaaagcgg atgattacac cggaccggca accgatctgc tgttaaagct
    11401 gcgcccgaac attcgttatt tccattcatc tcaatacatt aacgacctgg caaacggcga
    11461 tatttgcgtc gctatcggct gggcaggtga tgtctggcag gcgtcaaacc gcgcgaagga
    11521 agcgaagaat ggagtgaatg tctcgttctc gattccaaaa gaaggggcga tggcgttctt
    11581 tgatgtattc gccatgccag cggatgccaa aaacaaagac gaagcctatc agttcctgaa
    11641 ttacctgctg cgcccggatg tagtagcgca tatttccgac catgtgttct atgtcaacgc
    11701 caataaagca gccacgccgc tggtgagtgc ggaagtccgt gagaacccgg gtatttatcc
    11761 gcctgcggat gttcgtgcga agctgttcac tctgaaagtg caggacccga aaatcgaccg
    11821 tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa taatccgcag ccttagatgc
    11881 cgggcgggcg caccacaccc gccggcaatt cgcaccatca tggtgcgctt gcacacattc
    11941 aatgccggag agcagccgtg aatgacgcta tccctcgccc gcaggcgaaa acccgtaagg
    12001 cgctgacgcc gctattagaa atccgcaacc tcaccaaatc ctacgatggt caacatgcgg
    12061 tggatgatgt cagcctgacc atctacaaag gtgaaatctt cgcgctgctg ggcgcatccg
    12121 gctgtggcaa gtccacgctg ctgcgtatgc tggcaggttt cgaacaacct tctgccggac
    12181 agataatgct tgatggcgtc gatttgtcac aggttccgcc ttacctgcgc cccatcaata
    12241 tgatgtttca gtcttacgcg ctgtttccgc atatgaccgt ggaacagaac atcgcttttg
    12301 gcctgaaaca ggacaaacta ccgaaagcgg aaatttccag tcgggtcaat gagatgctcg
    12361 gcctggtgca catgcaggag ttcgccaaac gtaaaccgca tcagctttct ggtggtcagc
    12421 gacagcgtgt ggccctggcc cgaagccttg ccaagcgccc gaaactatta ctgctcgacg
    12481 agccgatggg cgcgctggat aaaaagctgc gtgaccggat gcagcttgaa gtggtggata
    12541 ttctggagcg cgtcggtgtg acttgtgtga tggtcaccca cgatcaggaa gaggcgatga
    12601 ccatggcggg gcgcatcgcc attatgaatc gtgggaaatt tgtccagatt ggcgaaccgg
    12661 aagagatcta cgagcatccg actacccgct atagcgccga atttattggc tcggtaaacg
    12721 tctttgaagg cgtactcaaa gagcgtcagg aagatggctt ggtgcttgat tcgccggggc
    12781 tggtgcatcc actgaaagtc gacgcggatg cctcggtggt cgataacgtg ccggtacatg
    12841 tggcgctgcg cccggaaaaa atcatgcttt gcgaagagcc gcccgccaat ggttgtaact
    12901 tcgcggtggg ggaggtgata cacattgcct atctcggcga tctttcggtg tatcacgttc
    12961 gtctgaaaag tgggcagatg attagtgccc agctacaaaa cgcccatcgt catcgtaaag
    13021 ggttaccgac ctggggcgac gaagtgcgtt tgtgctggga agtggacagc tgtgtggtgc
    13081 tgacggttta aggagcaaag atgaatacac ttgaacctgc tgcccagtcg aaaccgccgg
    13141 gcggatttaa gctgtggttg tcgcagctgc aaatgaagca tgggcgcaaa ctggtcattg
    13201 cgttgccata tatctggttg atcttgctgt ttctgctgcc atttctgatt gtctttaaaa
    13261 taagcctggc ggagatggcg cgcgctattc caccttatac cgaactgatg gagtgggctg
    13321 acgggcaact gtccatcact cttaatctcg gtaattttct gcaactgacc gacgatccgc
    13381 tctatttcga tgcttatctc cagtcgctac aggtggctgc gatttcgaca ttttgctgtt
    13441 tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg acccgtaata
    13501 ttttattact actggtgatc ctgccgtcgt ggacctcgtt tctgatccgc gtttatgctt
    13561 ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg ctgggggtta
    13621 tcgatcaacc gctgaccatt cttcatacca atctggcagt ttatattggc attgtttacg
    13681 cctacgtgcc gtttatggtg ctgccgattt ataccgcgtt gattcgtatt gattattcgc
    13741 tggtggaagc agcgctggat ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg
    13801 tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc ccggctgtgg
    13861 gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc gggcgcgtgc
    13921 tatggcaaga gtttttcaat aaccgcgact ggccggtggc ctcggcggta gcgatcatca
    13981 tgttgctgct gctaattgtg ccgataatgt ggttccacaa acaccagcaa aaaagcgtgg
    14041 gagaacacgg atgaataatt taccggtagt tcgttcgccc tggcggattg tgattttgct
    14101 gctgggcttt acctttctct acgcgccaat gctgatgctg gttatctatt cgtttaacag
    14161 ctcgaagctg gtgacggtgt ggggcggctg gtcaacgcgc tggtatggtg agttattgcg
    14221 cgatgacgcg atgatgagtg cggttggttc aagcctgaca attgctgcct gtgcggcaac
    14281 ggcggcggcg atcctcggga ctattgcggc ggtggtgctg gtgcgctttg gcaggtttcg
    14341 cggatcaaat ggctttgcct ttatgatcac cgcgccgcta gtaatgccag atgtcatcac
    14401 gggcttgtcg ctgttgttat tattcgtcgc gcttgctcat gccattggct ggcctgcgga
    14461 ccgcggtatg cttaccatct ggctggcgca tgtcactttc tgtacggctt atgtggcggt
    14521 cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagccg cgatggatct
    14581 cggtgcgacg ccgctgaaag tgtttttcgt cattacgcta ccgatgatca tgcccgcgat
    14641 catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga tcgccagctt
    14701 tgtttccggg ccgggagcca ccacgttacc gatgctggtc ttttccagcg tgcggatggg
    14761 ggtgaatccg gaaatcaacg ccctggcaac attgatcctc ggcgcggtcg gaattgtcgg
    14821 atttatcgcc tggtatctga tggcgcgcgc agaaaaacag cggatacgcg acatccaacg
    14881 tgcaagacgt ggctgaagac actaaaattt gccaacctgg ctacataatg ccgcgcatgt
    14941 cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac atcttcatct
    15001 catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg cgctcgccat tattatgatc
    15061 cgtggcctcg acgtgctgat gattttcaat acgctgggcg tgcgcggtat tggcgagttc
    15121 attcatcgca gcgtacaaac ctggagttta acgctggtct ttttaagcag tctggtgctg
    15181 gttttcattg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg
    15241 ctatatctgc tgacacaaat caccgccgca agttacttgt gggcggcttc gctggggtac
    15301 ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt ccatagcctg
    15361 atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc ctcgaccagt
    15421 cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca
    15481 atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca
    15541 gccgattcca gagcaactct ccgctaaaac cgccgatctt aaaaatctgc tcgccgattt
    15601 tccggttgag gaatggtgcg cgccggtgtc aggcccggaa caagggtttc gtaataaagc
    15661 caaaatggtg gtgagtggta gcgttgaaaa aacactgctc ggtatgctgc atcgcgatgg
    15721 cacgccggaa gacctttgtg actgcccgct ttatccagcc tcatttgcgc ccgtttttgc
    15781 ggcgctaaaa ccgtttatcg cccgagcggg gttaacgccc tacaacgtgg cgcgtaagcg
    15841 tggcgaactg aaatacattc tgctgactga aagccagagt gatggcggca tgatgctgcg
    15901 ctttgtactg cgttctgata ccaagctggc gcaactgcgt aaggcgctgc cgtggttaca
    15961 cgaacaacta ccgcagctga aagttattac cgtcaatatt cagccggtac atatggcgat
    16021 tatggaaggg gagacggaga tctacctgac cgaacaacag gcactggcgg agcgttttaa
    16081 tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg cggtcgccag
    16141 ccagttgtac gccaccgcgc gcgactgggt acgacagctg ccggttaaac atatgtggga
    16201 tctgttctgc ggtgtggggg gctttggttt acactgcgcg acgcctgaca tgcagttaac
    16261 cgggatcgaa attgcgccag aggccattgc ctgtgcaaag cagtcagccg ctgaactggg
    16321 cttaactcgt ttacaatttc aggcgctgga ctccactcag tttgccactg ctcaggggga
    16381 ggtgccggag ctggtgctgg ttaacccgcc gcgccgcggc attggtaaac cgctgtgtga
    16441 ttatctttca acgatggcac cgcgttttat tatctactcc agctgtaacg cccaaaccat
    16501 ggcgaaagat atccgcgaac tgccaggtta ccgcatcgaa cgggtacagc tttttgatat
    16561 gttcccgcac accgcgcact atgaagtgtt gacgctgctg gtgaagcaat aaaaaagcca
    16621 caggtgcggc ttcagattgc tgatcaggtg cgctttgttc atgccggatg cggcgtgaac
    16681 gccttatccg gcctacaaaa tcatgctaaa tcaatacatt gcagagttga tgtgggccgg
    16741 ataagcgcag cgcatcaggc aatcttactt ttgtcatcag tctcaattac tgcggaaacc
    16801 actggtcatt gattttctga tatgtaccgt cagctttaat tgctgccagc gcgttattca
    16861 gtttttccag cagagctttg ttatccggac gtacagcgat acccaggccg gtgccgaagt
    16921 attgcggatc ggtcactttt tcagtcgcaa cgcccagttg cggattggtt ttcagccatt
    16981 cgtttaccac cgcggtgtca ccaaataccc catcaatacg accatttttc agatcgataa
    17041 aggcattctg ataactgtca taggagacag ttttcacttc cgggtgctga tcctgaatat
    17101 atttctggtg cgtcgtaccg ttttccatcc caatgcgttt gcctttcagg tcggcaaacg
    17161 ttttgtaggt atcttttttg gaaatcacga cggctgagtt ttcatagtag ggtgtggtaa
    17221 acgaaacctg cttgctacgc tccggggtga tatccatacc ggagattacg gcgtcatatt
    17281 ttctgaattt cagtgacggg atcaggctgt cgaacgcgtg attagtaaaa gtacattctg
    17341 cctgcatttg tttgcacaag gcttttgcca gatcgatatc aaagccgaca atctcattat
    17401 tagcacctat agattcaaag ggtggatagg tggctgaaac gccaaaattg attttctctg
    17461 cggcagaagc accgaaagta aaggaagcaa gtaaagcggc aagaactaac tttttcatga
    17521 tggaactccc gtctgtcaat cttatgattt ttggccgtgt ctgcggcatg ggataacaat
    17581 gccatcaagt gaatttatat gcaataaaca tgattaaata atttaaatga aataaaaaaa
    17641 gacggataac ttagtgagtt gtccgtcttc attataagaa tttatgcact atgtaggccg
    17701 gataaggcgt ccacgccgca tccggcacag gcaccgtgct gatgtctgat gcgacgctgg
    17761 cgcgtcttat cagacctaca aacccccccg gcgaatgtac gcagctacat taattccgcc
    17821 gttcgaatgc cagtgctttg cgctcgatca gacgcatcat cagcgtcagc aggccgttga
    17881 cgaccaggta aataatccct gccgcaccga acaccattac atcgtaggtg cgtccgtaca
    17941 acaactggct gtatcccatc acttccatca gcgtaatggt gtaagccaga gaggtacttt
    18001 tgaacaccag caccacttcg ttggaataag aagagagcga gcgtttaaag gcatacggca
    18061 gcaggatcgc cagcgtatct tttttgctca ttcccagggc gctacaggac tgccactgac
    18121 cttctgggat cgcacgaatt gcaccgtaaa acagctgcgt ggtatacgcc gcactgttca
    18181 gcgataacgc aatcagcgca cataaccacg gttctgacaa caaatgccac agtgccggat
    18241 actcctgcaa cgtcggaaac tggcccggcc cgtaataaat caggaagatc tgcaccagca
    18301 gcggcgtacc ggtaaacagc gtgatataac cccgcaccag ccacaccagc accggcgttt
    18361 tcagcgtcag gatgatggta aaaatcaatg ccagaatcag tgccacaatc agcgaggcaa
    18421 cggttagcgt caggctggtg tgcagccctt tcatcagctc gggtaaatac tcaaacatta
    18481 gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact
    18541 gagcagggtg atcaccaggt aaatcgccgc cgccacaatg taccaggtaa atggttcctg
    18601 ggtacgagta gcaatgcttt tggtttgtaa cattaaatca ttcacactaa tcaaactgac
    18661 cagcgcggta tctttcagca gcaccagcca ctggttaccg aggccaggca gcgcatgacg
    18721 ccacatctgc ggcatcacca gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg
    18781 accagattcc cactggccca ccggtacagc tttcaacgcg ccgcgcagcg tttgcgaggc
    18841 ataggcggca tacagcagtg acagagcgat gacaccacaa aggaacggac tcacgtcgaa
    18901 gttctcaatg tccatctgca ccgggatctg cacgaaccca agattgatag tgaagccatc
    18961 cgaaagcgtc agcagtaact gcgaggagcc aaaatagata aacagcacta ccagaatttc
    19021 tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc cacgcgacag gacgccattt
    19081 cgctgactcc cataccgcaa agaacatcgc cagcgccagc ccgacaatca atgcacaaac
    19141 ggcaaggccg acggtcatcc cggcggcgct tgctaaagga aaaaattcat tcatcaggaa
    19201 ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg ccatctttct tcactttttc
    19261 cagcgcagtg ttgagtttct gctgcagttc agtgtttccc tgacgtaccg cgatgccgag
    19321 gccagtaccg aaataatctt tatcggtcac tttatcgccc actgccgcca gttttgggtt
    19381 atctttcagc cactcagtga ccactgcggt gtcaccaaat acgctgtcga tacgcccgtt
    19441 ttgcagatcc agttttgcgt tctggtagct gtcatacgga acggtggtga tttccgggtg
    19501 cttatccata atgaacttct ggtgtgtcgt cccgttctga acgccgactt ttttgccttt
    19561 cagctgatca acactggtgt atttgccttg ctgacccaca aacagggcag agttgtcata
    19621 gtacggggta gtaaacagta cctgcttttc acgctccgga gtgatatcca tgcccgccat
    19681 cactgcttct acgcgacgga atttcaggct tgggatcagg ctgtcaaacg cctggttaga
    19741 gaaagtacag gttgcatcaa tctctttaca cagtgcctgc gccaggtcga cgtcaaaacc
    19801 aacgatctgg ttgtttgcat caatcgattc aaacggagga tagggggctt cggtagcaaa
    19861 acgaatggtt tcggcagctg tggcggaaag actaaaacct gcaattaacg cggcaatcag
    19921 aacttttttc attgttgtta tcccgaatct tagtgagaga gatagttttt aaatgcttcg
    19981 gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta cgatatgacc attttccata
    20041 tacaccactc ggctggcggt tttacgcgcc acttcaactt cgtgggtgac gatcacctgg
    20101 gtaatattcg tttctgccag ctcacgaatg atgctgacga tttgtgccgt aatttccggg
    20161 tccagtgcgg cggtcggttc atcgaacagc agtacctgcg gttccatcat caacgcacgg
    20221 gcaatagcaa cacgctgctg ctgaccacca gaaagatgca gcgggtaacg atcgctataa
    20281 ggtttgagac gcagacgttc cagcagtttt tctgcacggg ccagcgcctg atctttactc
    20341 aaccccagta cacggcaggg cgcttcaatc aggttttgct gcacggtcag atgcggccac
    20401 aggttgtatt gctgaaacac catgccaacg ttacgacgca aatcgcgaat cgctttgtca
    20461 gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc
    20521 atctcaagca gattgagtac acgcagcagc gagcttttac ccgcgccgct ggggccaagt
    20581 aacaccagcg tttcgccctg tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg
    20641 ccgtagaagc aattaatgcc gtttaattga atactcattg acactcgtat actggcagtc
    20701 tgatagctat tgaggtcgaa gatagtacct ttgacagaat aattatgcaa tatttatgct
    20761 ttaaaagtta aaagcaaagc gcattattca ataaacatag cacaaaataa cgggggcggt
    20821 ggtcggtgag cataaatgtc ggcattcctc acgaaatgcc ggataattta cggggtttat
    20881 tggttgatca aggcgttagc gattctcgat ggactgacgg agcgtacccg ccgtggcatg
    20941 aacgctaccg cctaagtagc gcacatcgtc gatgacccaa cactggcctt cctgaatcat
    21001 taacacttca tcctgccagc cctggtcacc ctgtttgaga tccacccgca atggaatgtt
    21061 acgggcatca cgattaggga tagtcgatgc actggcaacg tgggcgctgt ctggcaaggt
    21121 ggttcgactg gagaacggat cgctgcttaa caattcacga tgactattat cgcggctggc
    21181 atcgctaagt tgggttgcca gtttgtcact caaatagggg cgcagggcgg taatgtcatt
    21241 gctgcgatgt tgaatgcggt agtcgtagaa ttgttgagcc acactgtcag gccctccctg
    21301 gacacaggaa ccggtgcggg ggccgttatc tttataagct ggcgtgacgg tggtacaggc
    21361 actcagcagc agtgcacatg gaataagtag agatagtgaa cggtagcgca taatcatttc
    21421 cttattagct tgctgtctgc gtcacactac gaccaaacgc tatcagatta tttaatacat
    21481 tgtttttaat acaattctga aacaatctga acacctgatt gggtttgagt ccattgctgg
    21541 cgacccactc aaggttgctc gtagctgact tacttaaatt acccaaacaa tagacagtgt
    21601 aaaccatttg tgaggttgag cgtgttatat ggctgaaatg atcatttaag gtaaatatga
    21661 tagttctgaa atggtggtat aaatcctcct gtagaggtta agagcatctg caagaagcca
    21721 gtttcacatt tataacgcgt gataaatgaa tgcggttatt gtctaatcat tacaaaattt
    21781 ttcacatgcg ataagagatc ctgcgattaa gatgattgcc aaaattacgt tgtatttttg
    21841 aaaatgagcg aaatttgata gtcccagctt tgactctcga taatattatt tatgctacaa
    21901 ttgtgggtaa agaatttaag ctaatgaatt tgagtgaaga ataaattaaa ttgcttcagt
    21961 atttcgttaa ttaatataaa tacaaaaggc gttgtgcttt tataattata aagcgcgagg
    22021 agttcggtgg attatcgttt ttcttgggtg atttttaata tgataaacaa tatatctgat
    22081 caagcatcat catttccagg cacacaacta aatcaaagtg ataatttcct ggattcactg
    22141 agggaatttt tcgcgatatt aaatcccagt cgtaagggtg agttgtctac atgggatacc
    22201 atctacttgc atctgatatt agcaataaat gctgacagtg atctcataaa aaatgatgtg
    22261 ttgctcgcgg aaaatattcc atcagcgaat taccaattta atacattttt tagcaatact
    22321 ttcgagattg atgttaaaaa atatctgggt aaatcagaag ataacgaagt tgaaataaaa
    22381 gcgggtaatg aaagaatctc tattggaata cgtaatgtat cgaatggaaa acttgaaaga
    22441 caacagtttc ttttcccact ggactatgag aataaattac aagaacaatt agataagtat
    22501 tttacgattg aatcgcaccc attattatac aggtatacca ttggtagtaa gattgcgaat
    22561 gtgatttttg aaaagttata cagtcgtatt gactttaaca aagaacaata tatatccttt
    22621 ataaaggacg catttattca tttttatgat tatagcaggc gatatgctat tagtgagaat
    22681 atcgataaag acgctgtaac aaacaatatc gcattgatga gcacttttta tgacagtgat
    22741 aatacgtcag gtgaggtttt aaataatgat tttacagaag aagagtcatt tgaaactgcg
    22801 ttagatgtag agcatgcaat agtgcttgga tttgctgatg ataattttga aacaaaacct
    22861 gttcattatc aggatttatt aacacgattt agtgcctttc aagataccgt gtttaatctg
    22921 tttcctgaaa tgcactcttc gcattaccac gatatttgta gcgtaagtgt ggatatgaca
    22981 aagggaacgc aatgcatgat tcacttgatg gtgaatgaag aagttttcat gtctctgcct
    23041 gttccagttg ccacgatggt acgtgaagat gcaagtaatt tagtcaatct taaaacacta
    23101 ctcaatgatg gttgtttcat taaatatagt cattttaatg acgtggcatt aataaagcaa
    23161 aacataagta atctctattt atcacacact gttgtgaatg aaagcatatt gaaaaaatgt
    23221 tgttttgaaa acggcagttt aggtgatgtt aaaataacaa atagcaatgt aataaacagt
    23281 gcattcaaaa atatttcgtt tagatctgtt aaaattaata acgtaaatac tcattcttta
    23341 aaatttatta actgcaattt tttcaatgtt gatatgataa gagttaatct cagcaaatgt
    23401 ctttttcatg aatgtagtat gcatggtgtc aagataaaac cctggttgcc tgtaaaatgg
    23461 acaaaagaat taataagtga ttatttgtat ggctgtttac tatctctgta ctctatctgt
    23521 gcaagggata tatataatat gaacgccggg aataacgtga aggttgctgc ggatgctttc
    23581 cttgaaatta ttttctctct taaaaataaa tattgtatta aactattaag cgctcaggat
    23641 cgggcgttta tatacgagtt tgcaagaatg atcttcgctt atattaatga caaaagcatt
    23701 gagatcttat tactttcttg ttttgcggcg gctgaccaaa aagcaatcca gcggtataga
    23761 cctcagtcac aagatgggga agactttagg agtcatttac aatataagtt gcctctttcc
    23821 gctcattaat gttgttggtg caacataata agacaggtaa gcggtaccct ataatgaaac
    23881 caatatcggg ttatgcaacg gataatcgta aagacttaag tgggtgtttt tgacacaatg
    23941 agatgatttc ctaaactgat caaaaccttc ttcccaacat ggcatatcag tccgataatg
    24001 ctttagagat cgaaggctta acaaacaagg agatcgatca tgcaattttc tacaacccca
    24061 actctggaag gcctgaccat cgttgaatat tgcggtgtgg tgaccggcga agcgatttta
    24121 ggtgccaata ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgttcc
    24181 ggtgcgtatg aaaaagaact gcgtaaagca cgggagatcg cctttgagga attaggctcc
    24241 caggcgcggg cgctgggggc cgatgccgta gttggtattg atatcgacta cgaaacggtc
    24301 gggcaaaacg gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga
    24361 tgagaagagt cttctggctg gtcgctgccg ctctgttact ggcagggtgt gcaggcgaaa
    24421 aaggcattgt cgagaaagag ggatatcagc ttgatacccg acaccaggcg caggcggcgt
    24481 atccgcgcat taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg
    24541 cgacactgac tgataagcag gtcagctcgc attatctgat ccctgcggtg ccaccgcgat
    24601 acaacggtaa accgcgcatc tggcaactgg tgccggaaca agaactggcc tggcatgcgg
    24661 ggattagcgc ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg
    24721 aaaaccgtgg ctggcaaaaa tcatccggag tgaaatattt tgccccgttt gaacaggcac
    24781 agattcaggc gcttattccg ctggcgaaag atattattgc ccgttatcac atcaagccgg
    24841 aaaacgtagt ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg gggccattat
    24901 ttccctggca gcaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtga
    24961 acttttacct tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc
    25021 ttttggcgcg ctacggttat gacgtaaaac ctgatatgac accgcgcgag cagcggcgcg
    25081 tgattatggc attccagatg catttccgcc cgacgttata taacggcgaa gcggatgcag
    25141 aaactcaggc gattgccgaa gcattgctgg agaaatacgg gcaggattag cgcggcagtt
    25201 ttccgtggtc gcgtagccag gcggcagttt tctcgatgcc ttcatccagg gtgatgaccg
    25261 gctgataacc taactcttcc tgcgcacgcg taatatccag cgtaaagtca aaattcaact
    25321 tggaggcgcc gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca
    25381 tgctgcgggc gatcatatcc agcatcgggt aagggacgga acgaatacga cagtcaatat
    25441 tcaactcgtc gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtga
    25501 tgttgtacac acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacattg
    25561 cgtgcacggc attttcatag taggtcatat cgaccagcgc actgccgcca tgcggtaaca
    25621 gaatactgcc gtagtggtgc atcatatgcg ccagacgggg aataaagact ttatcgtgcg
    25681 gtccgaacag actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa
    25741 gcatattgat cacttcttcg ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt
    25801 gagggcgaaa atcttcttta atattgcgat ggtggtgata atcgaagtac agggaggggg
    25861 aagagatatg aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag
    25921 tggcgcgaac gttagccaga tcgaaagcct gttgtgtccc ccagggtgag gtaaagctgg
    25981 agcagtgcca cagcgtatca atgcccgcga gcatcacttt agcttgtgat gaaaccagct
    26041 cggtcagatc cgtcggaaca aactctgcgc ccattttttc cagcaattta cccattgctt
    26101 cgttgcgacc gctcgcacgc acgctgatgc ctttctggca taaaaactct accgcgtttc
    26161 gacctaagcc gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag
    26221 aaaatgacgt gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa
    26281 taacgcaggt tttgtcgatt aatctgtgct ttgttctgcc agtctggcga tttgttttgc
    26341 cattccgcga aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg
    26401 cataccgtgc ggatgccaga aagcgcggac atcgatagta cgatagtcac ctttatcttc
    26461 cagggtaaaa cacagtcgtc ccagccccgg cgctttcatg ccaaataaca acgtaagttg
    26521 ctttttcggt tcaacgacaa tcactttcca gctatccacc gcatcgccgg tctgtaaata
    26581 ttcgcgctcc gggcggcctt tcgccagatt atgaccgatc gcgcggtcca tcaacgcccg
    26641 tgtctgccac aaaatattgc caaagaaata acgctcttta ccgccgattt ggttcaccac
    26701 cagccataaa gccgcaaggc tggcggacgt tttaacggta aaccccgcct gtttggcgaa
    26761 ataaccgtac tccggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga
    26821 gttgaccagt ttttcttcct ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag
    26881 ccgttgtggg atgagtgcac gtagcgcggt atcatctgcc agcagatcgt gtttcagccc
    26941 ctgaatcaac gccctggcgg tggtgggcgg tacggaagta atcacattga gaaaccacac
    27001 cgaaatccag cgggtgggga gggggatggg gatcaaccag cggcgcttac cgctcaccgc
    27061 cataaaatgt tcaaactgtt gctgataact gagcacctct ggtccggcgg cttcgaagat
    27121 gcggtgttcg ttggctggat ggtctaacag cgccaccaga tagtgcagca agttttccag
    27181 cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc gttaacactg gcaggttgta
    27241 gaccatatcg cgcatgactt cgaacgccgc tgaacctgcg ccaacgataa ttccggcccg
    27301 aagttcggtc actggtacat tcgcttcacg aagaatgtcc gccgtagcct gacgagctcg
    27361 cagatgatcc gactgctcat gtggcggggc ctgcaacgaa ctgagaaaga ttaattgctt
    27421 aactggtact tcacgtagcg catcgcggac gttgagagcc agctggcgct cctgagcgat
    27481 aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag acggtatcga tatcctgcaa
    27541 cagggccgga aggttatccg gccagctgag atcgacttta tggcaactga cgtttgccag
    27601 ttgcagcttt gcaagcctgt cgacatgacg tgccgccgct aggatctgat gcccttgctg
    27661 gctgagtgtg cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg
    27721 ttgcggcacg tctctctcct taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg
    27781 ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa
    27841 tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc
    27901 gaacaaacag catattagtg tcctgacgca tcacatccgc gcctgcttca cgcagttgct
    27961 ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg tgcgacgtta ttttccaggg
    28021 catacatccc ggcggcagcc agaatcccgg actgacgcat cccgccaccg gccattttcc
    28081 gccagcggat ggcacgttta atgtaatcac gattaccgac gagtaatgaa ccgactggcg
    28141 tcccaagacc tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca
    28201 gttcgcagcc gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca
    28261 gattgcgctc gcgggtaaat tcccatgctt ctttcaggta ttcacgcggc agcactttgc
    28321 cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt
    28381 tgattttcat cgccacttta tccagcggta gcgtgccgtc ggcagccgcg tcgatgggtt
    28441 gcggctgaat actgcccagc accgctgcgc caccggcttc aaacagatag ttatgcgcgg
    28501 cctgaccgac aatatactct tcgccacgtt cgcagtgact gagcagagcg accaggttgg
    28561 cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcatagt
    28621 cctgcagagc attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca
    28681 tcatcgcttc gagcatggcg cggctcggtc gggtaacggt atcactgcgt aaatcaatca
    28741 tggcatgtcc ttattatgac gggaaatgcc acccttttta ccttagccag ttcgttttcg
    28801 ccagttcgat cacttcatcg ccgcgtccgc tgataattgc gcgcaacata tacaggctaa
    28861 aacctttggc ctgttcgagt ttgatctgcg gtggaatggc taattcttct ttggcgacca
    28921 ccacatccac caacaccgga ccgtcgatag agaaggcgcg ttgcagggct tcatcaactt
    28981 cagaggcttt ttctacacgg atacccgtaa tgccgcacgc ttcggcaatg cgggcaaagt
    29041 tggtgtcgtg cagctcagta ccgtctgtca ggtaacctcc ggccttcatc tccatcgcca
    29101 caaagcccag cacgctgtta ttaaagacga taattttcac cggcagtttc atctgcacta
    29161 ctgagaggaa atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct
    29221 gacgttccgg ctcggttgcc tgcgcaccca acgcctgcgg catggcgtta gccatcgaac
    29281 cgtggttaaa cgaacctaac aggcgacgct tgccgttcat tttcagataa cgtgccgccc
    29341 ataccgttgg cgtgccgacg tcacaggtaa agatggcgtc atcggcggca aaatgactaa
    29401 tttgctgcgc cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca
    29461 gccctttgcg ggcatcgcgg taatcttcca gcgctttatc cagaaacttg cgatcggttt
    29521 tttcttccac cagtggcagc aatgcgcgca gggttgattt gatatcgccg accagcgcca
    29581 tatctacctt gctatgcgcg ccgatgctgg ctggattaat atcaatctga ataattttgg
    29641 catcggtcgg gtagaacgca cggtagggga actgagtacc gagtagcact aacgtgtcgg
    29701 cgttcatcat ggtatggaaa cctgacgaga atccgattaa cccggtcatt ccaacatcat
    29761 acggattatc gtattcgaca tgctctttac cgcgtagggc atgaacaata ggtgctttaa
    29821 ttttcccggc aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca
    29881 gggcgatatt gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg
    29941 gcatcacgac tggctgtggc gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt
    30001 ttaacgccac gtcgcccggt aatacaacaa cggaaacgcc acggttaagc accgctttgc
    30061 gcatagcaat tgccagcact tgtgggatct gctccgggct ggaaaccagc tcgcaatagt
    30121 gactacattc gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgccaattt
    30181 cgctggaggg aatatgagcg gcaatcgcca gtaccggaac gttattgcgg tggcaatcga
    30241 ataggccgtt gatcaagtgc aggttgcctg ggccgcacga tccggcacag accgccagtt
    30301 ctcctgaaag ttgtgcttca gcgccagcgg caaacgccgc cacttcttca tggcgggtgg
    30361 acatccactc gatggtgccc atgcgattaa gactgtcgct aagaccgttc agagagtcgc
    30421 ccgtgactcc ccagatgcgt ttcacgcctg ccgattcgag tgttttggcg atataagctg
    30481 caaccgtttg tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc
    30541 atctgacgga gggggaaggg atgggagaga aaggaggcac taacggttaa atagcccgat
    30601 gaaaggaata tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc
    30661 aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtactgc
    30721 tcaccgtata ttcaccggaa accacttttg tcttacagca gccgcaaaca cccgcacggc
    30781 aggcagcgac aaccggaacg ttattgcttt ccagcgcctc cagtagcgtg gtgccaaccg
    30841 gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt
    30901 ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt
    30961 cctgctctac ccaatccata tacggagccg ggccgcaggt catcacggta cgtgaagcta
    31021 agtcaggtac atctgccagc agttcgcgag tgaggcgacc agcgataaag ccttcggtaa
    31081 cgttattttc tgccaccagc gttaccggat agttacgcca ctcatcggcg aaaataacat
    31141 cctgcggcgt acgcacgttg tagatcaccc gcacatcggc ctgtggacgg ttcttcgcaa
    31201 gccagcgacg catcgacata atcggcgtga cgccgcagcc tgccgccagc aacaggaatt
    31261 tatcttctgc tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc cagagataat
    31321 caccgcgttt tacatcgcgc gtcagccact gggagccgac accgtcatca atccgccgca
    31381 cggtcagggt gatatattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg
    31441 tttccgctga gtttcgcacg ctgaccagtg catattgccc ggcgcgatat gggtagtgat
    31501 cgtggcaaat cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct
    31561 gtatccgcca cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgttcaa
    31621 cagttgcttc atgtcttctt caacagtggt gatagaacgc aggccgaatt tctcgttcag
    31681 caccgccagc aggtctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt
    31741 cacgcccaga gaaagcagcg tcagcagaat gacaatcgct ttctgttcaa accaggagag
    31801 caccagcgac agcggcagat cgttcacacc acagcccagt ttctctgcca gagtgacagc
    31861 cagaataatt gctgagtaag catcgttaca ttgacctgca tctaccagac gcggcagacc
    31921 ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta ccacaggcga gggtcaggat
    31981 caggcagtca tccggcacgc tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc
    32041 gccgtcacag ccaccaagca ggaagatatg acgcagtttt tcacggctaa ccagatcaat
    32101 cagcgtatcg gctgcaccaa gcagcgtctg gcgaccaaag cccacggtga tcaggtgagg
    32161 aatttcgctg tacgggaagc ccgccatttg ttgcgcctgg gcgatgaccg cagagaaatc
    32221 ttcgccatcc agatgacgca cgccaggcca gccaacgatg ctgcgggtcc agatacgatc
    32281 gtcataagcg cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg
    32341 gaaacgagcg aactccactt gctgattctg ccagccgctg ccgtagttac cgaccagatg
    32401 cttgaattta cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac
    32461 attaacgccc gtgccttcgg tctgttccag caggttgtag agatctttga gatcgtgacc
    32521 ggaaatcaga atgcatttac ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg
    32581 accgtattta ccggtttcgc ctgcatccag aatgctcatc actttgaagt tcatctggcc
    32641 gatttccatt gaacactcaa gaagcgcgtt catatcggca ggccaggtcc ccagccacgc
    32701 catgatttta tggtactggg cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg
    32761 ttccatatag gccgccgcac ctttcaggcc atacaggcac agcagacgca ggccgagaat
    32821 gttttcgcca atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag
    32881 ctcgccgaga tcatcgctca ccagttgcag gtcagccatc gggttatcga cgcgcgcgtt
    32941 ggcatctaca gccaggcatt gtgctttcag cgcctcgcgc aaggcaatcg cttcacgagc
    33001 gtagccgaca atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaaggcacg
    33061 tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca
    33121 ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga aggtcagaag tttccgccgt
    33181 tttaccacac atcccctgcg cgtatgagca gccgttttct gccggagtac ggatagtttg
    33241 ttcacattgc acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatgttta
    33301 agggtaagac gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg
    33361 atttagcgca attttggcgg cagggaacta ccgccagaga ggtattacgc agagaaaaag
    33421 gcgatgagga tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc
    33481 atatccagcc cgccagtacg ttgaagaacg ggcagggtga aatccattga tgtggcaccg
    33541 cataagccca gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa cataatagca
    33601 atcagttcac gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat
    33661 tcggtcaata aaataccgga aagagaatac cagccgaaac cggaggccat tgccagcgcg
    33721 gtattgatgg ggagatcaag aataaaggcg ttaattaaac caccaattaa tgaactgaca
    33781 accaccacca cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg
    33841 ccattattgc gcaactgaat accaacgagg aaaagtagca aaattaacgt gtattcactg
    33901 gcttcggtcg cgtgttgtaa gaaagccagt ccactcagac caatggcaaa accaatcact
    33961 actacgccgc acagttttag cgactccagc gccatcgcaa tacgcgacgg gagtttttct
    34021 tgctgatggt ggttgcgcca cggcaggcct cgctccagcc acatcagggc ggcaatatta
    34081 cacagtaaaa taacggtaat actgacggca gaataatgca gaatcgccaa cagg
//
LOCUS       DAJVPT010000033        33935 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVPT010000033 DAJVPT010000000
VERSION     DAJVPT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..717)
                     /locus_tag="OQM47_001563"
     CDS             complement(<1..717)
                     /locus_tag="OQM47_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1479762.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLP"
     gene            809..895
                     /locus_tag="OQM47_001564"
                     /pseudo
     CDS             809..895
                     /locus_tag="OQM47_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1300..1776)
                     /locus_tag="OQM47_001565"
     CDS             complement(1300..1776)
                     /locus_tag="OQM47_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1479763.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1835..3124)
                     /gene="bioA"
                     /locus_tag="OQM47_001566"
     CDS             complement(1835..3124)
                     /gene="bioA"
                     /locus_tag="OQM47_001566"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1479764.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3211..4251
                     /gene="bioB"
                     /locus_tag="OQM47_001567"
     CDS             3211..4251
                     /gene="bioB"
                     /locus_tag="OQM47_001567"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1479765.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4248..5402
                     /gene="bioF"
                     /locus_tag="OQM47_001568"
     CDS             4248..5402
                     /gene="bioF"
                     /locus_tag="OQM47_001568"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1479766.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5389..6144
                     /gene="bioC"
                     /locus_tag="OQM47_001569"
     CDS             5389..6144
                     /gene="bioC"
                     /locus_tag="OQM47_001569"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1479767.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6137..6814
                     /gene="bioD"
                     /locus_tag="OQM47_001570"
     CDS             6137..6814
                     /gene="bioD"
                     /locus_tag="OQM47_001570"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1479768.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7393..9414
                     /gene="uvrB"
                     /locus_tag="OQM47_001571"
     CDS             7393..9414
                     /gene="uvrB"
                     /locus_tag="OQM47_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1479769.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9606..10514)
                     /gene="yvcK"
                     /locus_tag="OQM47_001572"
     CDS             complement(9606..10514)
                     /gene="yvcK"
                     /locus_tag="OQM47_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1479770.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10779..10923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10911..11900
                     /gene="moaA"
                     /locus_tag="OQM47_001573"
     CDS             10911..11900
                     /gene="moaA"
                     /locus_tag="OQM47_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1479771.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11922..12434
                     /gene="moaB"
                     /locus_tag="OQM47_001574"
     CDS             11922..12434
                     /gene="moaB"
                     /locus_tag="OQM47_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1479772.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12437..12922
                     /gene="moaC"
                     /locus_tag="OQM47_001575"
     CDS             12437..12922
                     /gene="moaC"
                     /locus_tag="OQM47_001575"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1479773.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12915..13160
                     /gene="moaD"
                     /locus_tag="OQM47_001576"
     CDS             12915..13160
                     /gene="moaD"
                     /locus_tag="OQM47_001576"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1479774.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13162..13614
                     /gene="moaE"
                     /locus_tag="OQM47_001577"
     CDS             13162..13614
                     /gene="moaE"
                     /locus_tag="OQM47_001577"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1479775.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13751..14455
                     /locus_tag="OQM47_001578"
     CDS             13751..14455
                     /locus_tag="OQM47_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1479776.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14660..15373
                     /locus_tag="OQM47_001579"
     CDS             14660..15373
                     /locus_tag="OQM47_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1479777.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15409..16365)
                     /locus_tag="OQM47_001580"
     CDS             complement(15409..16365)
                     /locus_tag="OQM47_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1479778.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16365..17606)
                     /gene="clsB"
                     /locus_tag="OQM47_001581"
     CDS             complement(16365..17606)
                     /gene="clsB"
                     /locus_tag="OQM47_001581"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1479779.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17603..18364)
                     /locus_tag="OQM47_001582"
     CDS             complement(17603..18364)
                     /locus_tag="OQM47_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1479780.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18497..18907
                     /locus_tag="OQM47_001583"
     CDS             18497..18907
                     /locus_tag="OQM47_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1479781.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18869..19975)
                     /locus_tag="OQM47_001584"
     CDS             complement(18869..19975)
                     /locus_tag="OQM47_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1479782.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19986..21119)
                     /locus_tag="OQM47_001585"
     CDS             complement(19986..21119)
                     /locus_tag="OQM47_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1479783.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21112..22848)
                     /locus_tag="OQM47_001586"
     CDS             complement(21112..22848)
                     /locus_tag="OQM47_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1479784.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22841..23836)
                     /gene="hlyD"
                     /locus_tag="OQM47_001587"
     CDS             complement(22841..23836)
                     /gene="hlyD"
                     /locus_tag="OQM47_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1479785.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23839..24510)
                     /gene="cecR"
                     /locus_tag="OQM47_001588"
     CDS             complement(23839..24510)
                     /gene="cecR"
                     /locus_tag="OQM47_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1479786.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24739..25926
                     /gene="rhlE"
                     /locus_tag="OQM47_001589"
                     /pseudo
     CDS             24739..25926
                     /gene="rhlE"
                     /locus_tag="OQM47_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26133..26615)
                     /gene="ybiA"
                     /locus_tag="OQM47_001590"
     CDS             complement(26133..26615)
                     /gene="ybiA"
                     /locus_tag="OQM47_001590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1479787.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26735..28885
                     /gene="dinG"
                     /locus_tag="OQM47_001591"
     CDS             26735..28885
                     /gene="dinG"
                     /locus_tag="OQM47_001591"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1479788.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28913..29875
                     /gene="ybiB"
                     /locus_tag="OQM47_001592"
     CDS             28913..29875
                     /gene="ybiB"
                     /locus_tag="OQM47_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1479789.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30016..31101
                     /gene="hcxB"
                     /locus_tag="OQM47_001593"
     CDS             30016..31101
                     /gene="hcxB"
                     /locus_tag="OQM47_001593"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1479790.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31330..31590)
                     /locus_tag="OQM47_001594"
     CDS             complement(31330..31590)
                     /locus_tag="OQM47_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1479791.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31855..32121)
                     /gene="ybiI"
                     /locus_tag="OQM47_001595"
     CDS             complement(31855..32121)
                     /gene="ybiI"
                     /locus_tag="OQM47_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1479792.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32195..32872)
                     /gene="ybiX"
                     /locus_tag="OQM47_001596"
     CDS             complement(32195..32872)
                     /gene="ybiX"
                     /locus_tag="OQM47_001596"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1479793.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32914..>33935)
                     /locus_tag="OQM47_001597"
     CDS             complement(32914..>33935)
                     /locus_tag="OQM47_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1479794.1"
                     /translation="HPDSRIHPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNGG
                     IRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAKSGNLVNWKAGALYHL
                     TENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKR
                     LLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNITPAWQVIGGYTQQKA
                     TIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGARYIGSIHKGSDGAVGT
                     PAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLT
                     ANMHF"
BASE COUNT         8049 a         8709 c         9161 g         8016 t
ORIGIN      
        1 tggcaatgtg ttcagctgat tgttagggat atatagtgat tgcagacgtt gtggaagttc
       61 gggcagtctg tgtagttggt tttccccaac gtttaaaagc ctcagatttt gaggaagggg
      121 cggtagctct tgtaatctgt tatcaaaagc acttagttcc tgtagttcac atggtaattc
      181 tggtaaggca gtaagctggt tgttgccaac agaaagtttt tgtagagccg ggggaagatc
      241 aggtagcgtc tccaggctat tgagaagggc tgagagtgac tgcaaggaag atggaagaga
      301 aggcaaacat gttaaaactc tgttaagtga aacatcaaga gcgaccaggt gaggcggaag
      361 agccgggagc ctgctcagtc tgttatcgct ggctttaagt acagttaagg acggagggag
      421 ttctggcaga gagcgtagct cattgctaga gatgttaagt tcttgtatgt gcgggggcag
      481 gtatgggaga gagcgtaatc tgagtaaact taaattgagg gctggctctt gaaaagccag
      541 acatattttc agtaatcgaa ctgcctgtgt tcggtcttct gttgcagcac cttccttggc
      601 ccagttatcc cagatgcggt gataatccgt gatattttgc tcttctacgg ataaacggga
      661 tatacatcct aaaccacaaa aggagcggaa aaaagaagga ttgtgggcag gtttcattta
      721 ttattgatcc tcattgttta tatattaata tattcgtgca aatggagtga ggattcttta
      781 aaaaaacgcg cctgtaaata gttaagaaca ggaagaactg ttggggatgt ggctggccga
      841 aaatgaaggg gtgaagttct ggctcaatgt gctgactgaa ctgaaaaaac gcggttggtc
      901 gccctacctt ttcatcaagc cagtagtctg cccaccacag catcatttct ctgcgcttat
      961 cgagatactg agcatggttg taaattcagc taacccagtg cgttgacaaa aaattagcgc
     1021 aagaggacaa aaaatcacct tgcgctaatg ctctgtatca ggtcactaat accatctaag
     1081 tggttgattc atagtgactg gatatgttgt gttttgcagt attatgcagt ctatttttta
     1141 agctaaatgt attataatgc attgatatta atgattttta ctgtttctcg gtcagctttt
     1201 ttatactaac ttgagcgaaa cgggaaggta aaaagacaaa aagttgtttt taataccttt
     1261 aagtgatacc agatggcatt gcgccatctg gcagagtgat taactaaaca tcgcggtaat
     1321 cgatgcgctt gccagagagt ggaaatgaac gttaaacccg accatcgcgc cgctggctcc
     1381 ttcatcgaca tcaatacgct ctacatccag cgcgtgaacg gtaaaaatgt agcgatgggt
     1441 ttcgcctttc ggcggcgctg cgccatcgta tccggtttta ccaaaatcgg tacgcgtctg
     1501 caaaacgccg tctggcattg ctaccagacc agagccaaac ccttgcggta atacgcgggt
     1561 atcagcgggt aaattaacaa ctacccagtg ccaccagccg gagccggttg gcgcatccgg
     1621 gtcgtagcag gtgacaacaa aacttttcgt tcccgcagga acatcatccc acgccagatg
     1681 cggtgaaata ttatcgccat cgtaacccat gccgttaaag acatgacgat gcggcaactt
     1741 atcgccatcg cgcagatcgt tactgatgag tttcatgaac cctcctttct tgtttgcaga
     1801 aagtgtagcc agaaaccctc acgctgactt cccgttattg gcaaaaaaat gtttcatcct
     1861 gtaccgcgcg gttaaccgct gcggtcagac gctgcaactg ttgcgggaga ataatatagg
     1921 gcggcatcag gtaaatcagt ttgccaaaag gccggatcca taccccctgt tcgacaaaga
     1981 atttttgcag cgccgccata ttcaccggac gagtggtttc gaccacaccg attgccccca
     2041 gtacgcgcac atcggcaacc atttcggcat cacgggctgg tgccagttgc tcgcgcagct
     2101 gcacttcaat agccgccacc tgctgctgcc attcgccgga ttcgataatc gctaggctgg
     2161 cgtttgctgc cgcgcaggcc agcggattgc ccataaaagt tggcccatgc ataaagcagc
     2221 cggcttcgcc gttactgatg gtttctgcaa cctcgcgcgt ggtaagtgtg gcggaaaggg
     2281 tcattgtgcc gccggttaag gctttaccga ggcacaaaat gtccggcgcg atttctgcat
     2341 attcacaggc aaacagtttg ccggtacgac caaatccggt ggcgatctcg tcggcaatca
     2401 gcaagatacc ttcgcgatcg cacattttgc ggattcgttt taaccattcc ggatggtaca
     2461 tgcgcatccc gcctgcgccc tggacaatcg gctcaatgat caccgctgcg atttcatgac
     2521 gatgcgccgc catcaggcgg gcaaaaccaa ccatatcgcg ttcatcccat tcaccatcca
     2581 tgcggctttg cggggcggga gcaaacaagt tttctggcag atagcctttc cacaggctgt
     2641 gcattgagtt gtccggatcg cacaccgaca tcgcgccaaa ggtatcgcca tgataaccat
     2701 tgcggaaggt cagaaaacgc tggcgcgctt cgcctttggc ttgccagtac tgcaacgcca
     2761 ttttcatcgc cacttccacc gctacggaac cggagtccgc gagaaaaacg cactccagcg
     2821 gttgcggcgt cattgccacc agtttgcggc atagctcgat ggcgggcgca tgggtgatac
     2881 cgccaaacat cacatgcgac atggcatcaa tttgcgtctt catcgccgca ttaagctgcg
     2941 ggtgattgta gccgtggatc gccgcccacc aggacgacat accgtcaacc aggcgtctgc
     3001 cgtcagacaa aattagctcg caaccttcgg cgctcgccac cggataaacc ggcagagggg
     3061 aggtcatgga tgtgtatggg tgccagatat ggcgttggtc aaaggcaaga tcgtccgttg
     3121 tcataatcga cttgtaaacc aaattgaaaa gatttaggtt tacaagccta caccgaatta
     3181 acaacaaaaa acacgttttg gagaagcccc atggctcacc gcccacgctg gacattgtcg
     3241 caagtcacag aattatttga aaaaccgttg ctggatctgc tgtttgaagc gcagcaggtg
     3301 catcgccagc atttcgaacc tcgtcaggtg caggtcagca cgttgctgtc gattaagacc
     3361 ggagcttgtc cggaagattg caaatactgc ccgcaaagct cgcgctacaa aaccgggctg
     3421 gaagccgagc ggttgatgga agttgaacag gtgctggagt cggcgcgcaa agcgaaagcg
     3481 gcaggatcga cgcgcttctg tatgggcgcg gcatggaaga atccccacga acgcgatatg
     3541 ccgtacctgg aacaaatggt gcagggggta aaagcgatgg ggctggaggc gtgtatgacg
     3601 ctgggcacgt tgagtgaatc tcaggcgcag cgcctcgcga acgccgggct ggattactac
     3661 aaccacaacc tggacacctc gccggagttt tacggcaata tcatcaccac acgcacttat
     3721 caggaacgcc tcgatacgct ggaaaaagtg cgcgatgccg ggatcaaagt ctgctcaggt
     3781 ggcattgtgg gcttaggcga aacggtaaaa gatcgcgccg gattattgct gcaactggca
     3841 aacctgccga cgccgccgga aagcgtacca atcaacatgc tggtgaaggt gaaaggcacg
     3901 ccgcttgccg ataacgatga cgttgatgcc tttgatttta ttcgcaccat tgccgtagcg
     3961 cggatcatga tgccgacctc ttacgtgcgc ctttctgccg gacgcgagca gatgaacgaa
     4021 cagactcagg cgatgtgctt tatggcaggc gcaaactcga ttttctacgg ttgcaaactg
     4081 ctgaccacgc cgaatccgga agaagataaa gacctgcaac tgttccgcaa actggggcta
     4141 aatccgcagc aaactgccgt gctggcaggg gataacgaac aacagcaacg tcttggacag
     4201 gcgctgatga ccccggacac cgacgaatat tacaacgcgg cagcattatg agctggcagg
     4261 agaaaatcaa cgcggcgctc gatgcgcggc gtgctgccga tgccctgcgt cgccgttatc
     4321 cggtggcgca aggagccgga cgctggctgg tggcggatga tcgccagtat ctgaactttt
     4381 ccagtaacga ttatttaggt ttaagccatc atccgcaaat tatccgtgcc tggaagcagg
     4441 gggcggagca atttggcatc ggtagcggcg gctccggtca cgtcagcggt tatagcgtgg
     4501 tgcatcaggc gctggaagaa gaactggccg agtggcttgg ctattcgcgg gcgctgctgt
     4561 ttatctctgg ttttgccgcg aatcaggcag tcattgccgc gatgatggcg aaagaggacc
     4621 gtattgttgc cgaccggctt agccatgcct cattgctgga ggctgccagt ttaagcccgt
     4681 cgcagcttcg ccgttttact cataacgatg tcgctcatct ggcgcgactg cttgcttccc
     4741 cctgtccggg gcagcaactg gtggtgacag aaggcgtgtt cagcatggac ggcgatagcg
     4801 cgccactggc ggaaatccag caggtaacgc aacagcacaa tggctggttg atggtcgatg
     4861 atgcccacgg cacgggcgtt atcggggagc aggggcgcgg cagctgctgg ctgcaaaagg
     4921 taaaaccaga attgctggta gtgacttttg gcaaaggatt tggcgtcagt ggggctgcgg
     4981 tgctttgctc aagtacggtg gcggattatc tgctgcaatt cgcccgccac cttatctaca
     5041 gcaccagtat gccgcccgct caggcgcagg cattacgtgc gtcgctggcg gtcattcgcc
     5101 gtgatgaggg ggatgcacgg cgcgaaaaac tggtgtcact cattgcgcgt tttcgtgccg
     5161 gagtacagga tttgccgttt acgctggctg attcatgcag cgccatccag ccattgatcg
     5221 tcggtgataa cagccgtgcg ttacaactgg cagaaaaact gcgccagcaa ggctgctggg
     5281 tcacggcgat tcgcccgcca accgtacccg ctggtactgc gcgactgcgc ttaacgctaa
     5341 ccgctgcgca tgaaatgcag gatatcgacc gtctgctgga ggtgctgcat ggcaacggtt
     5401 aataaacaag ccattgcagc ggcatttggt cgggcagccg cacactatga gcaacatgca
     5461 gatctacagc gccagagtgc tgacgcctta ctggcaatgc ttccacagcg taaatacacc
     5521 cacgtactgg acgcgggttg tggacctggc tggatgagcc gccactggcg ggaacgtcac
     5581 gcgcaggtga cggcgttaga tctctcgccg ccaatgcttg cgcaagcccg ccataaagat
     5641 gccgcagatc attatctggc aggagatatc gaatccctgc cgttagcgac tgcgacgttc
     5701 gatcttgcat ggagcaatct cgcggtgcag tggtgcggta atttatccac ggcactccgc
     5761 gagctgtatc gggtggtgcg ccccggaggc gtggtcgcgt ttaccacgct ggtgcaggga
     5821 tcgttacccg aactgcatca ggcgcggcag gcggtggacg agcgtccgca tgctaatcgc
     5881 tttttaccgc cagatgaaat cgaagagtcg ctgaacggcg tgcgttatca acatcatatt
     5941 cagctcatca cactatggtt tgatgatgcg ctcagtgcca tgcgttcgct gaaaggcatc
     6001 ggtgctacgc atcttcatga agggcgcgac ccgcgaatat taacgcgttc gcagttgcag
     6061 cgattgcaac tggcctggcc gcaacaacag gggcgatatc ctctgacgta tcatcttttt
     6121 ttgggagtga ttgctcgtga gtaaacgtta ttttgtcacc ggaacggata ccgaagtggg
     6181 gaaaaccgtc gccagttgtg cacttttaca agccgcgaag gcagtaggtt atcggacggc
     6241 aggttataaa ccggtcgcct ctggcagcga aaagaccccg gaaggcttac gtaatagtga
     6301 cgcgctggcg ttacagcaca acagcagcct gcagctggat tacgcaacgg taaatcctta
     6361 caccttcgca gaatccacct cgccgcacat catcagcgcc caagagggca ggccgataga
     6421 atcattggta atgagcgccg gattacgcgc gcttgaacaa caggctgact gggtgttagt
     6481 ggaaggtgct ggcggctggt ttacgccgct ttctgacact ttcacttttg cagattgggt
     6541 aacacaggaa caactgccgg tgatactggt agttggtgtg aaactcggct gtattaatca
     6601 cgcgatgttg actgcgcagg caatacaaca cgccggactg actctggcgg gttgggtggc
     6661 gaacgatgtt acgcctccgg gaaaacgtca cgctgaatat atgaccacgc tcacccgcat
     6721 gattcccgcg ccgctgctgg gagagatccc ctggcttgca gaaaatccag aaaatgcggc
     6781 aaccggaaag tacataaacc ttgccttgtt gtagccattc tgtatttggt taaattgcga
     6841 gcgagatcgc gtcttcgatt gactgcaatt taaccaatta aattctaaaa taatcacgaa
     6901 aaaaatttta cttccgcctc atgcggcgaa tgtgggaatt gcccaggcgg cgggggatag
     6961 gggctggaga cagttatcca ctattcctgt ggataaccat gtgtattaga gttagaaaac
     7021 acgaggcaag cgagagaata cgcggcttgc acgcgaattg gcgttaaaga cggctcaaag
     7081 aaatatcttt tattttttaa ctggttagat aaatgcaatg gcagtcactg aacaggcatc
     7141 tcttgccata aaactgtcat cactcatctt gacaaatgtt aaaaaagccg ttgctttggg
     7201 gataacccgg taaggctgga gttttatctc gccacagagt aaattttgct catgattgac
     7261 agcggagttt acgctgtatc agaaatatta tggtgatgaa ctgttttttt atccagtata
     7321 atttgttggc ataattaagt acgacgagta aaattacata cctgcccgcc caactccttc
     7381 aggtagcgac tcatgagtaa accgttcaaa ctgaattccg cttttaaacc ttctggcgat
     7441 cagccagagg cgattcgacg tctcgaagag gggctggaag atggcctggc gcaccagacg
     7501 ttacttggcg tgactggctc cgggaaaacc ttcaccattg ccaatgtcat tgctgacctt
     7561 cagcgcccaa ccatggtact tgcgcccaac aaaacgctgg cggcccagct gtatggcgaa
     7621 atgaaagagt tcttcccgga aaacgcggtg gagtatttcg tctcctacta cgactactat
     7681 cagccggaag cctatgtacc gagttccgac accttcattg agaaagatgc ctcggttaac
     7741 gaacatattg agcagatgcg tttgtccgcc accaaagcga tgctggagcg gcgtgatgtg
     7801 attgtagtgg cgtctgtttc cgcgatttat ggtctgggcg atcctgattt atatctcaag
     7861 atgatgctcc atctcacggt cggtatgatt atcgatcagc gcgcgattct gcgccgactg
     7921 gcggagctgc aatacgctcg taatgatcaa gcattccagc gtggtacttt ccgcgttcgt
     7981 ggcgaggtga tagatatctt cccggcagaa tcggatgaca ttgcacttcg cgtggaactg
     8041 tttgacgagg aagtggaacg attgtcgtta tttgacccgc tgaccgggca gattgtttcc
     8101 actattccac gttttaccat ctacccgaaa acgcactacg tcacaccgcg cgagcgcatc
     8161 gtacaggcga tggaggagat caaagaagaa ctggccgcca gacgcaaagt gctgttggaa
     8221 aacaacaaac tgctggaaga gcagcggctg acccagcgta cccagtttga tctggagatg
     8281 atggacgagc tgggctactg ttcggggatt gaaaactact cgcgcttcct ctccggtcgt
     8341 ggaccgggtg agccaccgcc gacgctgttt gattacctgc ctgccgatgg gctgctggtc
     8401 gtcgatgaat ctcacgtcac cattccacaa attggcggca tgtatcgcgg tgaccgggcg
     8461 cgtaaagaga cactggtgga gtacggcttc cgcctgccat cagcgctgga taaccgtccg
     8521 cttaagtttg aagagttcga agcattagcg ccgcaaacca tctatgtttc ggcgacgccg
     8581 ggtaattacg agctggaaaa atccggcggc gatgtggtgg atcaggtggt gcgtccaacc
     8641 ggattgcttg acccgattat cgaagtgcgg ccggtggcga cacaggttga tgatcttctt
     8701 tcggagattc gtcagcgagc ggcaattaac gaacgcgtgc tggtcaccac actgaccaag
     8761 cgaatggcgg aagatcttac cgaatatctc gaagaacacg gtgagcgcgt gcgttatctt
     8821 cactcagata tcgacaccgt cgagcgtatg gagattatcc gcgacttgcg tctgggtgag
     8881 ttcgacgtat tggtagggat caacttactg cgtgaaggtc tggatatgcc ggaagtgtcg
     8941 ctggtggcga tcctcgacgc tgacaaagaa ggcttcctgc gttccgaacg ttcgttgatc
     9001 cagaccattg gtcgtgcggc acgtaacgtt aacggtaaag cgattctcta cggcgatagg
     9061 agcactccat caatggcgaa agcgattggc gaaaccgaac gtcgccgtga gaaacagcag
     9121 aagtacaacg aggaacacgg cattacgccg caaggcttga acaagaaagt ggtcgatatc
     9181 ctggcgctgg ggcagaacat tgccaaaacc aaagcgaagg gcagaggaaa atcgcgcccg
     9241 attgttgagc cggataatgt gccgatggat atgttgccga aagcgttgca gcagaaaatc
     9301 catgaactgg aagggctgat gatgcaacac gcgcagaatc tggagttcga agaagcggca
     9361 caaattcgtg accagttgca tcagttgcgt gagttgttta ttgccgcatc gtaacaggat
     9421 agcgaagaag actgatgaca aacggaaaac agtctgatgc gctacgctta tcaggcctac
     9481 attttctccg caatatattg aatttgcgcg gtttgtaggc cggataaggc gttcacgccg
     9541 catccggcat aaacaacgcg cactttgtct tcaataggaa gccggaattt tccttcggat
     9601 ttccgttaac ctaaagcctg taacgccttt tccagcgcgt tatgtaacaa ctggcggtca
     9661 tgacgatacg gaatatcgct ggcctccagt acctcctgga tcacaatccg ctctttcacc
     9721 gccgagacat ccacttttgg cccgacgatg accgcatcaa tgaccttttt accaacatac
     9781 tgctccataa ttgccagctt gctttccagc ttcaaattag ccgcaggtaa actcaactca
     9841 cgccccagat tgccgatata aaccatcggc gctggcgtgc ggcgtaatgc ctgggcgatt
     9901 tccttcagca gcagaattgg catcaggctg gtataaaaac tgccaggccc aataatgatg
     9961 agatccgctt cattgatagc gtgaaccgcc tcacgcgttg cgggtacatt aggcgttaac
    10021 aataactctt gaatcggcgt agttaactgg tcgatattga cctcgccgta aacttcatgc
    10081 ccctgatcgt caatcgccat cagatcaaca ggatgctctg acattggaat caaatgcgta
    10141 tccactttca gcagattacg aattaaattg atggcttcca gaggccgcac gctaaggtga
    10201 tccagcgcct ttaacatcaa gtttccgaga ttatgaccgg aaagttcgcc attgccgcca
    10261 aaacggtatt caaacatcgc ggaggcgacg ttcggttccg ttatcagctg gttgaggcag
    10321 ttgcgcatat cgccccaagc aatgccgcct tctgaacggc gaatacgccc cgtcgagcca
    10381 ccattatccg tggtggtgac gatacccgtt aaacgagaac ccaaagacga aagtgatgag
    10441 agaacgcgtc ccagtccatg ccctccgccg agagcaacga cacgatcaag atcagccagc
    10501 gtacgattgc gcatatagtt tcctggagtc agattatccg cgctacagta gcgcaaattg
    10561 cggggaaaca gcaattaacc tgccaatatt gaggatacaa actattttct tctatctcta
    10621 aaggacgatg cacgctatgc ctccctgatg atgtatatca aagtaaaacc gccattttcc
    10681 cttattctgt agcgaaatag cacgatcatg acgctatata tatgattaca tagcgaaagt
    10741 gtggatggta aaaatctcat ttacacgcta gtatcggctt aaccactaaa cactctagcc
    10801 tctgcacctg ggtcaactga tacggtgctt tggccgtgac aatgctcgta aagattgcca
    10861 ccagggcgaa ggaagaaatg acttcgcctc ccgtatctgg aaaggtgtac atggcttcac
    10921 aactgactga tgtatttgcg cgtaagtttt actacttgcg cctgtcgatt accgatgtgt
    10981 gtaactttcg ttgcacctac tgcctgccgg atggctacaa accgagcggc gtcaccaata
    11041 aaggctttct taccgtcgat gaaattcgcc gggttacgcg cgccttcgcc agtctgggca
    11101 ccgaaaaagt gcgcctgaca ggaggagagc cgtctttacg ccgcgacttt accgatatca
    11161 tcgccgctgt gcgggaaaac gacgctatcc gccagattgc ggtcaccacc aatggttacc
    11221 gtctggaacg cgatgtggcg aactggcgcg atgcgggact tactggcatt aacgtcagtg
    11281 tcgacagttt ggacgcccgc cagtttcacg ctattaccgg gcaggataaa ttcaaccagg
    11341 tcatggcagg gattgatgct gcatttgagg ccggttttga gaaggtcaaa gtcaataccg
    11401 tgctgatgcg tgatgttaat catcaccagc tcgacacctt tctgaactgg atccagcatc
    11461 gccctatcca gctgcgtttc atcgaactga tggaaacggg cgagggcagc gagctcttcc
    11521 gtaagcatca catctctggt caggttctgc gtgacgagct actgcgtcgc ggctggatcc
    11581 accaattacg tcaacgcagc gacggtcccg cgcaagtctt ttgccatcca gattacgccg
    11641 gagagattgg ccttatcatg ccgtatgaaa aagacttctg cgccacttgc aaccgcctgc
    11701 gcgtttcctc cattggtaaa ctccatctct gcctgtttgg tgaaggcggc gttaacctgc
    11761 gcgatctgct ggaaggcgat acccagcaac aggcgctgga agcgcgtatt tcagcggcgc
    11821 tgcgggagaa gaaacagacc catttcctgc atcaaaacaa caccggtatt acgcaaaact
    11881 tatcgtacat tggcggctaa aacgtcaaaa ggagagatca gatgagtcag gtaagcactg
    11941 aatttatccc gacccgtatt gctattctta cggtttctaa tcgtcgcggt gaagaagacg
    12001 atacctccgg tcactatctg cgcgattcgg cgcaagaagc gggccatcac gttgtcgatg
    12061 aagccattgt gaaagaaaac cgctacgcta ttcgcgctca ggtatctgcg tggatcgcca
    12121 gcgacgatgt acaagtggta ttgattacgg gtggtactgg cctgacggaa ggtgatcagg
    12181 ctcccgaagc gctgctgccg ttgttcgacc gtgaggttga aggttttggt gaagtgttcc
    12241 gtatgttgtc gtttgaagag attggcactt ccacgttgca atctcgtgcg gtagcgggcg
    12301 tcgctaacaa aacgctgatt ttcgccatgc cgggttcgac caaagcgtgc cgtaccgcat
    12361 gggaaaatat catcgcgccg cagctggatg cccgtacgcg tccgtgtaat ttccatccac
    12421 atttgaagaa ataagtatgt cgcaactgac ccatatcaac gccgctggcg aagcgcacat
    12481 ggtggatgtc tccgccaaag cggaaaccgt gcgtgaagcg cgggcggaag cctttgtcac
    12541 catgcgcagc gagacgctgg cgatgattat tgatggtcgc caccacaaag gcgacgtatt
    12601 tgccactgcg cgtattgccg gtattcaggc ggcaaaacgc acttgggatc tgatcccgct
    12661 gtgtcacccg ctgatgctca gcaaagttga agtcaattta caggccgagc cggagcacaa
    12721 tcgggtgcgt atagaaacct tatgccgcct gaccgggaaa accggtgtcg aaatggaagc
    12781 gttaaccgcg gcctccgtgg cggcgctgac catttatgac atgtgcaaag cggtgcaaaa
    12841 agatatggtg attggtccgg tacgtttgct ggcgaagagc ggcggcaagt cgggtgactt
    12901 taaggtggaa gcggatgatt aaagttcttt ttttcgccca ggtgcgcgag ttggtgggaa
    12961 cagatgcaac cgaagtggct gcggattacc caaccgttga agcgttacgc cagcacctgg
    13021 ctgcgcagag cgatcgctgg gcgctggcgc tggaagatgg caaattgctg gctgccgtca
    13081 accagacgct ggtgagtttt gaccatccgc tgactgacgg cgacgaagta gctttcttcc
    13141 cgccggtaac cggaggttaa gatggcagaa accaaaattg ttgtcggccc gcagccgttc
    13201 agcgtaggag aagagtaccc gtggctggcg gagcgtgacg aagacggtgc ggtagtcacc
    13261 ttcaccggaa aggtgcgcaa ccataacctg ggcgacagcg tcaaagcatt aaccctcgaa
    13321 cactatccgg ggatgactga aaaagcactg gcagaaattg ttgatgaagc gcgtaaccgc
    13381 tggccgctgg ggcgcgtcac ggtgattcac cgcatcgggg aattatggcc gggcgatgaa
    13441 atcgtttttg tcggtgtcac cagtgcgcat cgcagcagtg cgtttgaagc cgggcagttt
    13501 attatggatt atctcaaaac ccgcgcaccg ttctggaagc gcgaagccac gccggaaggc
    13561 gaccgctggg ttgaagcccg ggagagcgat ctgcaggcgg caaaacgctg gtagtttttt
    13621 gttagccgga taaggcaccg ctgcgtccgg caatttcacc gctcagtaca tgcgtttacg
    13681 ctgcgtcgat gcacagcctc atcattttgc agtatcctta agatattcct tatatcttca
    13741 ggagatcgtc atggacagat tcccacgttc tgattcaatc gtacaaccct gggctggctt
    13801 gcaaacttat atggcgcaag tctatggctg gatgaccgtt ggcttgttgc tgaccgcatt
    13861 tgttgcctgg tatgcggcta attccgcggc cgtgatggag ctgttgttca ctaaccgtgt
    13921 ctttttaatc ggtctgatca tcgcgcaatt agcattggtt attgtgttat cagcgatgat
    13981 tcaaaagctg agcgcaggtg taacgacgat gctctttatg ctttattcgg cgctgacggg
    14041 tcttacgctt tccagtatat tcattgtcta taccgctgct tctatcgcca gtactttcgt
    14101 cgttactgcc gggatgttcg gcgcaatgag cctgtacggt tacaccacga agcgcgattt
    14161 aagtggcttt ggcaatatgc tgtttatggc gttaatcggc attgtgctgg catcgctggt
    14221 caacttctgg ctgaaaagcg aagcattgat gtgggcagtt acctacatcg gcgtgattgt
    14281 ctttgtcgga ttgacggcgt atgacactca gaaattgaaa aatatgggtg agcagattga
    14341 tacccgcgac acgtcgaacc tgcgcaaata ttccattctt ggtgcgttaa ccttgtatct
    14401 ggacttcatc aacctgttcc tgatgttgtt acggatcttc ggcaaccgcc gttaattctt
    14461 tttcaccatc actatcccgg ccatgtgtcg ggattttttt cttactaaaa acgacactta
    14521 acaagtacca ggtataaaat atgccttatg agcgagcgga atcgttttcc gtatccataa
    14581 atacaaaagt attttgtatg cgtggtttta aatttattga tggtgaatat taatgttttt
    14641 tctaaggaca actaaaagaa tggaatcata ctcgcaaaac agcaataaat tagattttca
    14701 gcacgaggcc aggatattaa acggtatatg gctcattacc gctttaggtt tggtggcaac
    14761 cgcaggacta gcctggggag ctaagtatct cgaaattacg gcaaccaaat atgattcacc
    14821 accaatgtat gtcgccatag ggttattatt actttgtatg tatggcttaa gtaaggatat
    14881 caacaagata aatgccgcca tcgcgggcgt aatatatctg tttttactct ctttggtggc
    14941 gattgtcgtt gcaagtttag ttcctgtata tgccattatc atcgtgttca gcactgcggg
    15001 cgcgatgttt ttaatcagta tgctggccgg tttattattt aatgttgatc ctggttctca
    15061 ccgttttatc attatgatga cgttgacagg gttggccctg gtaatcatcg tgaatgcggc
    15121 attaatgagt gaacggccca tttgggtaat aagttgctta atgattgtgt tatggccagg
    15181 cattatctcg catggacgaa ataagctcct tgaactggca ggaaaatgcc atagtgaaga
    15241 gttgtggagt ccggttcgtt gcgcttttac aggtgcatta acactctatt actattttat
    15301 cggcttcttt gggatacttg ccgcgatagc tataacgctt gtctggcaaa ggcatacgcg
    15361 tttttttcat tagtcgtaaa tgcagatact aaagaactta actaaacttc acatcgccgc
    15421 ttcatttttc gcccgtagct tcttcgcttg gctttccagt agcaggtaac agatcagcgc
    15481 cagcagcagc gggataaagt aatacaggac gcgataagcg agcagggcgg cgataatgct
    15541 ccctttcgag gtatgctccc cagccagtag tgcgataaac accgcttcca gcacaccgat
    15601 ccccgccgga atatgcacga tgacgccagc aatactacta accagtaaca cgcccagtac
    15661 aaagaaatag ttcacgcttt gaccaagtaa cagccagata atcgccccca ttaccatcca
    15721 gttaacgctg gaaatcagca tctgagcgag ggcgaatttc catgaaggca gcaccagctt
    15781 ttgccctttg atggtcatat ggcggtgctt cgcgaaagcg caaaaccaca aataaaccgc
    15841 gataatcatc agtaagccaa tgccgagaat acgcagcgta gtttgatcga cataccagta
    15901 atccggcaac tccaccacgc ctgcggtaaa gataatccct gccagtaaaa tatagcccag
    15961 ccagttggtg gtaatactga gcgaggaaat ccgcgtaata gtgctgcccg gtaaccctag
    16021 tcgggagtac aaacgatagc gcataccaat gccgccgacc caggtactga gcgtcaggtt
    16081 gaaggcgtag cagataaacg acaccagcat cacctggcgc ttcgccagtt tgtgaccgca
    16141 gtaaaagcgg gcgagcaggt catagcagcc gtaaatcaga tagctgacga ccaccagccc
    16201 gaccgcacta agcagcgcaa cgcgattgta gtcgcggatg accttccaga cctcttccca
    16261 gtccactttt ttggcgtaga ccaccagcaa cacaatcacc gcgataaaaa acagccaggt
    16321 gaggatcttc tttgctaagc gccagcgcgg gtgtgattta ctcatcaggg gtttaccccc
    16381 gtgttttcag tttctacccg atcctgcgtt tccattgtcg gttgtgcggg gggatcaacc
    16441 tgcgtcagac gtggtgtgtg tgccggaagc cagccaacca gcgccgggaa gtggcgtaaa
    16501 aagtggaacg ccagcacgct tttggtcagg ttccaccagg tgcgtttggg cagcatggtt
    16561 tcatccacct gctgacaatc tgcggcaata atgccgttca gattatcgcg cagcgtctgg
    16621 ttaaaatgac gatcgtggat gatgacattt gcttcgagat tcagtgacaa actgagcgga
    16681 tcgagattac tggaccctac tgtcgcccag tgatcgtcca tcaatgccac tttgccgtgg
    16741 agcgggcggc ggcggtactc aaaaacctga acgccgcctt taaccagata gttatacagc
    16801 aagcgcgcac cgactctgac aatcggcata tccggttcgc cctgaatgat cagtttgatc
    16861 cgcaccccgc gccgtgccgc tttacgcaag gcgtgtaaaa atcgatagcc ggggaagaag
    16921 taggcgttgg cgataatcac ttcccgctgc gcctgagtga gcattttcag ataatggcgt
    16981 tcaatatcat cgcgatgttc ttcgttatcg cgccagacca gcaatacctg cgcttctccc
    17041 ggctggcggt tctcttccgc tttgtgatga cgtcgccacc agcgtcgtgc cgcgctctgt
    17101 ccaggcaggt tttccagctc aaactggaga atatcttcga caatcggccc ttcaaggcgt
    17161 accgcgtaat cctgtttagc ctctggaccg tagctggaca tatgctcggc ggagtaattc
    17221 agcccgccaa taaaggcgat acgcgcgtcg atcaccacaa ttttgcgatg catccggcga
    17281 aacacattgg tgcgcatacc aaaaaggcga gggcggggat cgtagtagcg gaacactacg
    17341 ccagctgccg tcagttcatt gacaaactca tcgctgagat ccggcgaacc gtagccatcc
    17401 agcaagactt ccgctttaac cccgcgttgc gctgctgcca gtagtgccgc atgcagctgt
    17461 ttgccgacgt tatcctcaaa ccagataaac gtttcaagaa tgatgcgttc ttgtgcctcg
    17521 ccaatcgcct taaacaccgc gggataatat tgctcgccgt tttccagcaa ctggatctta
    17581 ttgccttcgc gccagctaca tttcataaat gaatctccgc acttaaaggg gcatgatcag
    17641 aaaggtgtcg ccatgtccgc agcggcaacg cggttggcgc gctggcgctg gcatttttga
    17701 cgtagatcct gtccagtcgt agtagaggaa attgcaccgg aaacgtgcgc gccgggcgtc
    17761 cgtgggcgcg ggtaaaaatc tcatccagtc cggcctgcac ttttaacgga tgattagctt
    17821 tttgccgcca gtcattgaaa tcacccgcca ccaataccgg ttcgccgtcc ggtagctcat
    17881 tcacccattc ggcgagcatc gcaagctgcg cctgacggtg cgcctcacgc aggcccagat
    17941 gtacgcacat cacatgaatc gcttttccgg tcatcggcgg cacaatgcgg cagtagagca
    18001 cgccgcgctt ttccgcacca tcgaccgaaa catcgcgatt ctcataatgt tcaatgggat
    18061 aacgcgacag tacggcgttg ccgtgatgcc cttccgggta tacggcattg cgaccgtagg
    18121 caaaatcgct ccacatagtg tcggcgagaa actcgtagtg cgaggtatcg ggccagtttt
    18181 ccacatgcag cggatgaact tcgtgcgcgc ccatcacttc ctgcaggcaa acaatatcgg
    18241 cgctgacggt acgcacggca tcgcgaagtt ccggcaaaat gaagcgtcgg ttaaacgcgg
    18301 taaagccttt gtgaatgttg atggtgagca ccttgaacga aaattgttgt gtttgatcgg
    18361 gcataatttt cctgtctttg cctcttatct cattgaaata gtgtagtcgg cgtcacaaaa
    18421 aggtgcggtc ttacggaatt ttccgtaaag ttcggtactc tgagtaagta gagataaatt
    18481 cttcaggaga gaagccatga agtggcaaca acgtgttcgt gtcgcaacgg gtctaagttg
    18541 ctggcagatt atgttgcatt tactggtagt ggcgctgctg gtggtgggct ggatgagtaa
    18601 gactctggtt cacgtcggcg tgggattatg cgcactgtat tgtgtcacgg tagtgatgat
    18661 gctggtgttt cagcgccacc ccgagcaacg ctggcgtgag gtggcagacg tgctggaaga
    18721 gctgaccacg acctggtatt ttggcgcagc gctgattgtg ctgtggctgt tgtcccgcgt
    18781 actggaaaac aactttttgc tggcaattgc agggctggcg atccttgctg gcccggcggt
    18841 agtgtctttg ctggcgaaag ataagaagtt acatcacctt acgtctaaac atcgcgtacg
    18901 ccgctgaccc tgtcgtggcc gttatcacca gtagcggcca caaactattc cacacaatat
    18961 ccagactcgc atccttcaaa taaatctgct tggtaatgtc cgtaaagtgg cgaatagggt
    19021 taatccacgt cagattttgc agccacactg gcatgttttc caccggcgaa acgtaaccgg
    19081 aaaggagaat ggcgggcatc ataaaaacaa acacgccgat aaacgcctgc tgttgtgtcg
    19141 agcagagtga tgaaatcaac agaccgaatc ccaccagcga taaaccgtaa atcaccatcg
    19201 taaagtagaa cagcgctagc gatccggcga aggggatttg atacgcccag ataccaatcg
    19261 ccagcacaat ggtggcctgg aaggtggcga caattaacgc cggtacggct ttgccgatga
    19321 agatctgcca ggtggtgagc ggcgaaacca gtagctgatc gagcgtacct tgttcacgtt
    19381 cgcgggcgac ggaaagtgaa gtgacgatca ttacgccgat agtggtgatc atggcgatca
    19441 gcgacggcac cacaaaccat ttgtagtcga gattcgggtt ataccagttg cgtaccacca
    19501 gctcgctgtt gttgggtttc ggttttcctt ccagcagctc ctgctgataa tttttgacga
    19561 tctgttgcag gtagttggcg gcaatttgcg cactgttgga gttacgcccg tcgaggatca
    19621 actgcaacgg cgcggtctgg aaggtatcca gtttgcgcga gaagtcagcc gggaaacgca
    19681 ccagtagtaa cgccttttgt gtgtcgatgg tcgggcggat ctcctgtggg cttttcagca
    19741 gcagcacatg tgtaaaggcg ctggcgcggg caaaacgttg agtaagttcc accgaatgct
    19801 cgccgttatc ttcatcgtag atggcgatgg tggcgttagt cacttccagc gtggcggcga
    19861 acgggaacag gatcacctga attagcacgg gtaaaatcag aatcgcgcgg gtttgtggtt
    19921 cgcgcagcaa cgactgcaac tctttgcgga ttaacgtcca taagcgatga aacatgctct
    19981 tctccctaat ccagccgacg tttggttttc agccacgtca ggccgataaa catcaccgcc
    20041 gaagcgatca aaaacagcac gtttaccacc agcaccactg gaatattccc ggcgaggaac
    20101 aggctttgca gggtgctgac gaaataacga gcgggaataa tgtacgtcac cgcgcggatc
    20161 accgcgggca tactgtcgat ctgaaaaata aagccggaaa gcataatcga cggcagaaaa
    20221 gcggcgttca gggcgacctg agcggcattg aactggttgc gggtaatcgt ggaaatcagc
    20281 agccccattc ccagggtact gagtaaaaac aggctggaga taaaaaacag aatcagcagc
    20341 gaaccgcgat acggcacgcc gagaataaac actgacacca gcatacacag caacatcgcc
    20401 agcatcccga gaaagtaata agggatcagc ttacacagca gcagttccgt gcgggtaatc
    20461 tccgtagaga gcagagcctc catggtgccg cgttcccatt ctcgcgccac caccagcgag
    20521 gtgagaatcg cgccgatgac cgtcatgata atggtcaccg cacccgggat aataaagtgc
    20581 tggcttatcg ccgccgggtt aaaccagtag cgggtttgta catcaataag cggttcaaaa
    20641 gtctgcccgt tgtcctccgc tcgctgcatt tgccagatct gccagatccc ttcgacatac
    20701 ccctgcacaa agttagcggt attcggctca ctgccgtcgg tgatcacctg aatcggtgcg
    20761 gtggcgttgg cgcgctccat ctgttcggcg aaatccacgg gaataaccac cagaccgcga
    20821 attttccccg actgcatttt ggcgatcagt tcctgacggt tatcgctgat ggtggcgtcg
    20881 atgtagggcg aaccggtcat ggtgtgggtg aaatccagcg ccgcttcgct acgctgttcc
    20941 agcaaaatcc cgacccgcag cttgctggag tcgaggttaa tgccgtaacc aaaaataaac
    21001 agcagtagca gtgggatcac taccgcaatc agccagctac tcggatcgcg aacgatctgc
    21061 cgcgtctctt tgacgcacag cgcccgcacg cgatgccagg aaaggatcgg gttactcatt
    21121 gctatgctcc ttatcccagt cgtggatcaa ctgaataaag gcttgctcca tggtgggatc
    21181 gggttgctca tcgttagctg actgtgcttt caaatcgtcc ggcgtaccgc tggcgattaa
    21241 tttcccacgg tacaccaggc cgatgcggtc gcaatattcc gcttcatcca taaagtgggt
    21301 ggtgaccatc accgtgacgc ctttctctac catgctgttg atgtgcagcc aaaattcacg
    21361 gcgggtgagg gggtcaacgc cggaagtcgg ttcgtcgaga aagagaatat ccggttcatg
    21421 catcagcgaa caggccagcg ccagccgctg tttaaaacct aatggcagtt catcggtggc
    21481 gtgtgaggca atacttttca ggccaaatgc ctcgctcata cgggagattt tttcgttctg
    21541 cgcccgaccg cgtaagccat acacaccaga gaaaaagcgt aaattctgtt cgaccgtcag
    21601 gttgccgtag agcgaaaatt tttgcgccat atagccgaga tgctggcgcg ctttaccgga
    21661 actctctttc agatccatcc ccagcaccag cgcctggccg gaagtcggca ccagcaaacc
    21721 gcacatcatc ttaaaggtgg tcgatttacc cgcgccgttt ggccccagca aaccaaaaat
    21781 ctccccacgt ttaacggcaa agttgacgtg atcggtggcg gcaaaatcac caaatttctt
    21841 ggtcagttct ttcgcttcga tcaccgtctc gccgggtgtg ccttctaccg tatgcaatat
    21901 tgcacccagc ggcgattctg aggttccggc accgcccagc aaatcaataa acgcatcttc
    21961 aaaacgcggc gtagtttcgt tgatgttgat ttccggcatc ccgtcggcat ggcgaatatc
    22021 gtctggtgtg gcctcttttt tgaggatcag acgtaccgat ttcccctgaa tcatgccatc
    22081 gctgacctgc ggcagtttca aggcgcgttg caacagtttg cggttgccct cgtgtggact
    22141 ggtcatcaga aagctgcgtc cggccatggt ttgtgtcagg gcttttggtt ctccctgata
    22201 cagcaactcg ccttcgttca tcagtaacac gtcacggcac tgctcggctt cgtcgagata
    22261 cgaggtactc cagaggatta acatcccttc gcccgccagc tcatgcacca tctgccacag
    22321 ttcgcgccgt gagatagggt caacgccgac gccgggttca tcgagcagca acactttcgg
    22381 ttcgcccacc agggtacagg ccaggccgag tttttgtttc atcccaccgg agagcttgcc
    22441 cgccaggcgt ccggtaaacg gcccaagaga cgtaaactcc agcaggcgag caaaagtttg
    22501 cttacgtgcc tcgccggtga cgctgcgcaa atccgcgtac agattgaggt tctccatcac
    22561 cgtgagatct tcatacagac caaatttctg cggcatatag ccgagcacgg cgtgcagcgc
    22621 gccgtcgttt ttgatcggat caaagccaat caccgtggca ctgccgctgt cgggtttcag
    22681 taatcccgcc aacatccgca tcagtgtggt tttacctgca ccgtccggcc ccaccaaccc
    22741 cgtcacataa ccggcgtgaa tggtacaatc gagcggcgcg acggcgggct tgtccatgcc
    22801 cggaaagcgt ttttccaggc cgttcagcgt gataacggca tcattcatgt cctgcctcgt
    22861 caccgaattg taccgtcact ggcattccct ggcgtaacgc atcatcggcg tcggtcacca
    22921 caatacgcag gcgatagacg aggtcggtac gcagatccgg tgtttcgacg gttttcgggg
    22981 taaattcagc agtcggcgaa acgaaaccaa tctgcccgtg atacggcttg tccgggcgac
    23041 catcggtata aagcagcact ttgcgccccg gctgggcctg gtcaagattc cgttcatcaa
    23101 cataagcgcg cacccacacc ggacgcgtta gtgaaacggt aaacaccgtg ccaccttcat
    23161 tgagaaccgt gcctggctct accgcgcgcg ttaacagcgt gccatcagac ggtgctatca
    23221 acgttgagtc ctgtaaattc agctccgcct gcgccagttg cgcctgcgcc tgttcgaggc
    23281 tggctttcgc ctgagcgatg tcctgttcac ggttaccgga acggtactga cgcaatttat
    23341 cctgtgctga tttcagcgtt gcctgtgcct ggtcgcgcga ggagcgggca ttttccaggt
    23401 catttgccga aatggtgcgg cttttccaca gtccttgctg gcggttatag aagttctgcg
    23461 catagtcata ggcggcttgc gcctgtttca ccgctgcggc ggcctgagcg atttcttcat
    23521 cgcgataccc ggcaagcatc agatcatact gcgcctgtgc caccgagacg cccgctttcg
    23581 cctgcatcag ggcaatctca tacggcttgt gatccagttc gcccagcacc tggcccgctt
    23641 tgatagcatc accttcgtcc accgccagcg attcaacgcg ccccccaaca cggaaactaa
    23701 gattcaccgt acgaatatcc acgttgccat acagcgtcag gccgttatcc tggcggcttt
    23761 gataccacca gtagcctccg gcaaccacgg cggcaagtac cactaccgcc agtccgatca
    23821 cgacaggttt tttcatcact acaaactcct ttgcgataat ccttgcaaaa tcaggtcgat
    23881 atgacaggtc accgtctggt tgatcagttc ggtcttttct tcatcgaacg cggtccagcc
    23941 ggtacgtaac agaattgttt ctttgccaag acggaacgcc agaatctcac caatcaacgc
    24001 atgggtatga aggatcatgc gggtgtcatt agcgtcgcag ccggtccagg cggcaatcag
    24061 gcgtgtcagg tggctgtgta gcggactaat cacctgttcg tgcaccaggt ggtaggctgc
    24121 cgtgggagag agctgctcac gggagataaa cttgctgagg ttgacggtgt catcctgggt
    24181 gagcagctta atcatgttcc tgcaggcgcg aaggatcagt tcacggatgg cagcccgatc
    24241 aggctgtggt tgggcgaaca agcgttcggc ttcctcggca tgcggacgga actgctcgcc
    24301 aataaaatcg gcaatccact gggcgcaggc gaggtacaaa tcttctttcg aaccgaagta
    24361 gtaggtgatg gcagcgatat tctgcccggc ctgggcggct atctcgcggg tggtggcgtt
    24421 cattccatat tcaccaaact gcgccagtgc ggcagcaatc agctgttttt tcgcctgttc
    24481 acccttgatt gtcatggcag gattattcat cgcacagtcc attcttaatc aaatgattga
    24541 ttaagattat gactccatat gggagttgtc cagtatggct aagaatttta gctacgccag
    24601 tcacagggat aatttatgcg ctgcgtcaca aaaactgcta cactccgctc cctcatgaca
    24661 ttgtggtttt tgtcattttc cttttcagta tctccctgaa aactacaccg gtaacggtcg
    24721 gggcggttcg gagtagttat gtctttcgat tctttgggtt taagccctga tatcctgcgc
    24781 gccgttgccg agcagggtta ccgtgaaccc acccctattc agcagcaggc gatccctgct
    24841 gtgctggaag gccgcgacct gatggctagc gcccagaccg gcaccggcaa aacagcgggc
    24901 tttacgctgc cgctgttgca acacctgatc actcgccagc cgcacgccaa agggcgtcgt
    24961 ccggtacgtg cgctcattct taccccgacc cgtgaactgg cggcacagat tggcgaaaac
    25021 gtccgtgatt acagcaaata cctgaacatt cgttcgctgg tggtgtttgg cggtgtcagc
    25081 attaacccac aaatgatgaa gttgcgcggc ggcgttgatg tgctggtggc aacgccggga
    25141 cgtttgctgg acctggaaca tcaaaatgca gtgaagctgg atcaggttga aatcctcgtc
    25201 ctcgatgaag ctgaccgcat gctcgacatg ggctttatcc acgatatccg tcgcgtgtta
    25261 acaaaactgc cagcgaaacg ccagaacctg ctgttctctg cgaccttctc tgacgatatt
    25321 aaagccctgg cggaaaaact gttgcacaac ccgctggaaa tcgaagtggc acgccgcaat
    25381 accgcgtctg atcaggtgac ccagcacgtt cactttgtcg ataagaaacg caaacgcgaa
    25441 ttgctgtcgc acatgattgg taaagggaac tggcagcagg tgctggtgtt tacccgtacc
    25501 aaacacggcg ctaaccatct ggctgagcag ctcaataaag atgtcatccg tagtgtggcg
    25561 atccacggga ataaatcgca aggtgcgcgt acccgtgcgc tggctgattt taaatcgggt
    25621 gacattcgcg tactggtggc gaccgatatc gctgcgcgcg gtctggatat cgaagagttg
    25681 ccgcacgtcg tcaactatga actgccaaac gtaccggaag attatgtcca ccgtatcggg
    25741 cgtaccggtc gtgcggctgc taccggtgaa gcgttgtcgc tggtgtgtgt tgatgaacac
    25801 aaactgctgc gtgatatcga aaaactgctg aaaaaagaga tcccgcgcat tgcgattccg
    25861 ggctatgagc cggacccgtc aatcaaagcc gaaccgatcc agaacggtcg ccagcaacgt
    25921 ggcggcatca ggctgatgaa caaacgcaaa actgcctgat gcgctacgct tatcaggcct
    25981 acgtgaactc tgcaatatat tgaatttgca tgcttttgta ggccggataa ggcgtttacg
    26041 ccgcatccgg catttcacaa caagcacttg tcagcaattt gagaacacgg gaaaaaattt
    26101 atctgtttta ccgcccataa gccaccgaga cgttacttct ctatagccaa ttgttcgcgc
    26161 aactccatta aaaggtagcc cagtcgattc ttgcccttac cattaccacc gtctccccag
    26221 taagcatcgt tttccgtatg ctcaaccagt tttgcgggcg cggttgccag caagagcaca
    26281 cgcagttctg catgctgttc gaatttagcg cgaagtgctt tacgcatcac ctgttcttta
    26341 accgactccc agtttttacg aagtggcttg gaacgattac gccccatgcg agcggcgacc
    26401 atgggggaag aaacccgacg gatctcttcg cggtattttt catcaaggaa tttttgtgcc
    26461 tgaaaatagt gttcggaggt gggccaggta ttcccgtcaa ccttgatggg cctggcggca
    26521 aagttagaaa aatcaccgta gtcatcgctg gtgctgtaga aatttatgat ggtgtcttgc
    26581 atgacgtgtt ggatcctttg tgctcgaacg ggcattaaac cgcattatgt tggtggttat
    26641 tgcgagccgc tttccagaaa cagaaaaacc attacccctg aaaaccgaaa aatgccacaa
    26701 tattggctgt ttatacagta tttcaggttt tctcatggca ttaaccgccg cgcttaaagc
    26761 gcaaattgcc gcctggtata aggcgcttca ggaacagatc cccgacttta ttccccgtgc
    26821 gccgcagcgg cagatgattg cggacgtcgc caaaacgctg gccggagaag aagggcggca
    26881 tctggcgatt gaagccccca ccggcgttgg gaaaacgctc tcctatttga tccccggcat
    26941 cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt accgccaacg tggcgttgca
    27001 ggatcagatt tacagcaaag atttaccgct gctgaaaaag atcattcccg atcttaaatt
    27061 cactgccgct ttcgggcgtg ggcgctacgt ttgtccacgt aatctgacgg cgctcgccag
    27121 cactgaaccc acgcaacagg atctgctggc gtttcttgac gacgaactga cgccaaataa
    27181 tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat ctcgacactt ataaatggga
    27241 tggtctgcgc gatcatacgg atatcgctat agatgatgat ctctggcgtc gtttgagtac
    27301 cgacaaagcc agctgcctca accgtaactg ttactactat cgtgaatgcc cgttttttgt
    27361 tactcgtcgg gagattcagg aagcggaagt ggtggtggca aaccatgcgc tggtgatggc
    27421 ggcgatggaa agcgaagccg tactgcctga cccgaaaaat ctgctgctgg tgctggacga
    27481 aggtcatcac ctgccagatg tggcccggga tgcgctggag atgagcgctg aaatcaccgt
    27541 gccctggtat cgactacagc tggacttgtt cacgaaactg gtcgctacct gcatggagca
    27601 gtttcgcccg aagaccatcc caccgctggc gatccctgaa cgtttgaatg cgcattgtga
    27661 agagttgtat gagcttatcg cctcgttaaa caacattctc aatctctaca tgcctgccgg
    27721 gcaggaggca gagcaccgtt ttgcgatggg cgaactgcct gatgaattgc tggagatctg
    27781 ccagcggctg gcaaaactca ccgagatgct gcgtggtctg gcggagttat ttcttaacga
    27841 tttaagtgag aaaaccggca gccacgatat tgtgcgtctg catcggttga ttttacagat
    27901 gaaccgcgcg ctggggatgt ttgaggtgca aagcaaactc tggcggctgg cgtctctggc
    27961 gcaatcttcc ggtgcgccgg tgaccaaatg ggcgacgcgg gaagagcgcg aagggcagct
    28021 acacctctgg tttcactgcg tgggaatacg tgtcagcgat cagctggaaa ggctgctgtg
    28081 gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg cgttcgctga atagtttttc
    28141 gcgtttgcag gagatgagtg gtctgaaaga gaaagcgggc gaccgttttg tggcgctgga
    28201 ttcccccttt aaccactgcg aacagggcaa aattgttatt ccccggatgc gctttgagcc
    28261 ttccatcgac aacgaagagc aacacattgc cgaaatggcg gcctttttcc gtgaacaggt
    28321 ggagagcaaa aaatatctcg gtatgttggt actgtttgcc agcggacggg cgatgcagcg
    28381 ctttctcgac tatgtgacgg atttacgcct gatgttgctg gtgcagggcg atcagccgcg
    28441 ttatcgctta gttgaactgc accgcaaacg cgttgccaac ggtgagcgta gcgtgctggt
    28501 gggcttacag tcatttgccg aagggcttga tttgaaaggt gatctgctca gccaggtgca
    28561 tatccacaaa atcgcttttc cgcccatcga cagcccggtg gtgatcaccg aaggggaatg
    28621 gctgaaaagc ctcaaccgct atccgtttga ggtgcaaagc ctgccgagcg cctcgtttaa
    28681 cctgattcag caggttgggc gactgattcg aagccacggt tgctggggcg aagtggttat
    28741 ctacgacaaa cgcttgctga caaaaaatta tggcaagcga ctactggatg cattaccggt
    28801 atttccgata gagcaaccgg aagtccctga aggtatagtt aaaaagaaag aaaaaacgaa
    28861 atccccacgc cgtcggcggc gttaatgatg tgagtcaggt aaggagtcgt aaatggacta
    28921 tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac gcgcgcgatt tagaccggga
    28981 tactgcgcgc ggtctgtatg ctcatatgct caatggtgaa gtccctgacc ttgagttggg
    29041 cggcgtattg attgcgctgc gtatcaaagg ggaaggggaa gcagagatgc tcggctttta
    29101 cgaagccatg caaaatcaca ccatcaagct gacgccgcct gcaggcaagc cgatgccgat
    29161 tgtcatcccc agttacaacg gcgcgcgtaa acaggctaac ctgacgccat tattggcgat
    29221 tctgctgcat aaactcggtt ttcctgtggt ggttcacgga gttagcgaag atccaactcg
    29281 cgtgctgaca gaaaccattt tcgaactgat gggtattaca ccaacgttgc acggcgggca
    29341 ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg cctgtcggcg cgttttgccc
    29401 gccgctggaa aaacaactgg cgatgcgctg gcggatgggc gtgcgtaaca gtgcacatac
    29461 cctggcgaaa ctggcgacgc catttgccga aggtgaggca ctgtgtcttt ccagcgtttc
    29521 gcatccggaa tacattggac gcgtcgcgaa gttctttagc gatatcggcg ggtgggcgtt
    29581 actaatgcat ggtactgaag gcgaagtgta tgctaatccg cagcgctgcc cgcagatcaa
    29641 tctcattgac cgtgaaggga tgcgggtgct gtatgaaaaa caggaccctg ctggtagcga
    29701 gttactgcca caagcaaaag atccggaaac cacggcgcag tggactgagc gttgcctggc
    29761 tggcagcgaa gcgattcccg aatcgctgaa aatccagatg gcttgctgcc tggtggctac
    29821 gggtgaagcg gcaactatca gcgatggcct ggcgcgcgtt aatcaggcat tttaattttt
    29881 atctcagccg gatgagccac gtcatccggc ttttacccca ccgcaatatg aaattcctgc
    29941 atctttattg accttcccgc gcccggcgtg cagcataaaa aaacaacaaa cacataacat
    30001 aaacaggagt taaccatgga aagtggtcat cgctttgatg ctcagacgct gcacagtttt
    30061 attcaggctg tatttcgtca gatgggtagc gaggaacaag aagcgaaatt agttgccgat
    30121 catttaatcg cggcaaacct ggcagggcat gattctcatg gtattggcat gatcccaagc
    30181 tatgtgcgtt cctggagtca ggggcatctg caaattaacc atcatgccaa aatcgttaaa
    30241 gaggcggggg cggcagtcac gctcgatggc gatcgcgcat tcggtcaggt cgcggcacac
    30301 gaagcgatgg cgctggggat tgagaaagcg catcagcacg gtattgctgc cgtggcgcta
    30361 cataactcgc atcatatcgg ccgtatcggt tactgggcgg agcagtgttc agcggcgggg
    30421 tttgtctcta tccactttgt tagcgtggtc ggtattccaa tggtcgcgcc gttccacggt
    30481 cgcgacagcc gctttggcac caatcccttc tgtgtggttt tccctcgtaa agataatttc
    30541 ccgctgttgc ttgattacgc caccagcgcc attgcatttg gcaaaacccg cgtcgcctgg
    30601 cataaaggcg tccccgtgcc gccaggttgc ctgattgacg ttaacggcgt gccgacgacc
    30661 aatccggcgg taatgcagga gtcgccgttg gggtcgctgt tgacctttgc cgaacataaa
    30721 ggctacgccc ttgcagcgat gtgtgaaatt cttggcgggg cgctttctgg cggtaaaacg
    30781 acgcatcagg aaacgttgca aaccagcccc gatgccattc ttaactgcat gaccactatc
    30841 atcatcaacc cggaactgtt cggcgcgccg gattgtaacg cgcagaccga agcctttgcc
    30901 gagtgggtga aagcctcgcc gcatgatgac gataagccga ttttgctacc gggcgagtgg
    30961 gaagtgaaca cgcgtcgcga acggcaggag caggggattc cattggatgc gggaagctgg
    31021 caggccattt gtgatgcggc gcggcagatt ggtatgtcgg aagagacgtt gcaggctttc
    31081 tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg cggacgggca aacaagggta
    31141 acataggatc aatgagggtt agagcatatg cgtctgtcgg caaacagaca gggaaatact
    31201 tgtgctggac gtagcgtaaa cgcctgatcc gtccaacggt tcggattttg taggcctgat
    31261 aagacgcggt agcgtcgcat caggcatctg ttgccggatg cggcataaac gccttatccg
    31321 tcctacgaat tacttataaa ttaccgcagt accgcttaat ttattgttgt tggtggcgga
    31381 agtgatgctg tatccactgg caccggctgc cgccgctttc tcagccagtt tcgcttccag
    31441 ggcatcgagg gtggatgcgc catcggcaga aaccacgccg attttattca tgttctgtgc
    31501 ctgggatgcc gttaccggtt ccgccgcgaa cacgccaaat gacagagttg aaagagccat
    31561 agcagcaaca acagtattga tagttttcat aattaatctc tcgcaggtga tttttgtata
    31621 agtgacgttg tttcgtcgat gtgatgagta tcacgttttt ttacgagaga taaaatcgaa
    31681 gagaattgac cgccttgttc aaataaattg attgatatct aacatactga taaaccattt
    31741 atttgttgta cttatggact tgttttatgc cgcgtggcag tcacagcagc gttaaaggca
    31801 cggaatgcca cgcaaaatac aaattatact cgctatgcat ctcgccagtt gtagttaacg
    31861 taactgtctg tctttcgaac ctctgcgatt atatcctgta tacgctggtt tttgtaaatc
    31921 tttctcctgc tgacaatgaa tacataagcg cacgccagga atggcttccc gacgggcttg
    31981 cgggatgggg gcaccgcact cttcacattc atacaggctt tcgccacgcg gaatttcacc
    32041 ccgagcgcgg gcaatcgcat cttcaattgt actgttgatc tgttcgttga cggcgtcatc
    32101 gttagcccaa gcggatgcca tatcgtcctc cccatatcaa tgcttgtaca gttaagtgta
    32161 gctaatatag ggacgaacct gggcagttca agcgtcagat ctccgaccat tcccgcagca
    32221 gattatgata aagattgagc agcgacagga tctcttcatt ttcaccatag cggcttttca
    32281 gcgactgaat atttttgtcc agttcaaaca gcatggcgcg ctttttatca tcgcggatca
    32341 tcgactggat ccacataaat gatgccactc gtacgccgcg ggttacgggt gtcacgcaat
    32401 gcaggctgct ggagggatac aacaccagat cacctgccgg gagttttacc cgatgttgtc
    32461 caaaggtgtc attaacgacc agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca
    32521 gcgtggcaga aaggtcagtg cgcatccagc cgttttgtgg atggctgcgt actgcgccat
    32581 ccacatggaa accataggtt tcattgttct gatagcgatt aaacagcggc gtggaaaggg
    32641 tacgcggcaa ggcagcggca aagaacaatg catgttggtt aaccgcgttc agcacctcat
    32701 tttgcagtgc ggcgtataac gcgctgcggg tgtctacttg ctgattgttc ttaacttgtg
    32761 caccttgtgc tccggtggtg acgcgtccat ccacccattc ggcttgttcc agttgttcgc
    32821 gaaaatgggc gacgtcctgt ggcgataaca cgccgggaat gtggtacatc atacaagttc
    32881 tccaaaaagt ggggcctgcg ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa
    32941 gaaggttctt ggctcgcccg ggtgataacg gtagccgctc ttgttgattg aggcgacgta
    33001 atcggtatca aacaggttgt aaacgtttag ctggaagtcg agattgcgat taacccgata
    33061 ccccagtttg gcatcggcga cccagtaacc ttctgtaaat gctggcgttc ccaccgcgcc
    33121 gtctgaacct ttatgtatac tgccgatata gcgtgcgccc gcgccaacag agatatcatc
    33181 ggttgcctga tattggctcc ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga
    33241 ggaaccatcc tgggcaacat ctttgccgtt tttgatggtt gctttttgct gggtatagcc
    33301 gccaatcacc tgccacgcgg gagtgatatt ccctgccacg gatatctcat agccttcgac
    33361 gcgtttctta ccgtattgcg agtaagtccc gtcatcattt tgctcaactt cattttcgat
    33421 atcagtacgg aacagcgcgg cggtgagcaa caggcgttta tccagaacct gccatttggt
    33481 gccaatctcg ctggtgttgg ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc
    33541 actgccagac tgcgcaaggg cgaagttgtt gccgcccgga ggctgctggg aaacggcata
    33601 gttaatatag acattgccgt tttccgtcag gtggtacagc gccccggctt tccagttcac
    33661 cagattgccc gacttggcgg tgtcgacggt ggtgactgga gaaccttttg ccacaccagc
    33721 tgggcaggtg atggcaccgc gtccgctgcc gccgcaggcg gtggcactgt catattcagt
    33781 atgataatta tccagacgga tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt
    33841 atcgaaggcg taaattgcga aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt
    33901 caggccgccg ggatgaattc tgctgtcagg atgat
//
LOCUS       DAJVPT010000034        33715 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVPT010000034 DAJVPT010000000
VERSION     DAJVPT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM47_001598"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM47_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1479795.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM47_001599"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM47_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1479796.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM47_001600"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM47_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1479797.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM47_001601"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM47_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1479798.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM47_001602"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM47_001602"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1479799.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM47_001603"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM47_001603"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1479800.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM47_001604"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM47_001604"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1479801.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQM47_001605"
     CDS             complement(4598..5320)
                     /locus_tag="OQM47_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1479802.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM47_001606"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM47_001606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1479803.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQM47_001607"
     CDS             6520..6738
                     /locus_tag="OQM47_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1479804.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM47_001608"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM47_001608"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1479805.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQM47_001609"
     CDS             complement(7472..7672)
                     /locus_tag="OQM47_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1479806.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQM47_001610"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQM47_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQM47_001611"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQM47_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1479807.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OQM47_001612"
     CDS             10101..12014
                     /locus_tag="OQM47_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1479808.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13035
                     /locus_tag="OQM47_001613"
                     /pseudo
     CDS             12014..13035
                     /locus_tag="OQM47_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            13029..13247
                     /locus_tag="OQM47_001614"
     CDS             13029..13247
                     /locus_tag="OQM47_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1479809.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13301..14170
                     /locus_tag="OQM47_001615"
     CDS             13301..14170
                     /locus_tag="OQM47_001615"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1479810.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14225..14629)
                     /locus_tag="OQM47_001616"
     CDS             complement(14225..14629)
                     /locus_tag="OQM47_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1479811.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14931..15563
                     /gene="crp"
                     /locus_tag="OQM47_001617"
     CDS             14931..15563
                     /gene="crp"
                     /locus_tag="OQM47_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1479812.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15614..17704
                     /locus_tag="OQM47_001618"
     CDS             15614..17704
                     /locus_tag="OQM47_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1479813.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17771..18991)
                     /gene="argD/dapC"
                     /locus_tag="OQM47_001619"
     CDS             complement(17771..18991)
                     /gene="argD/dapC"
                     /locus_tag="OQM47_001619"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1479814.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19077..19640)
                     /gene="pabA"
                     /locus_tag="OQM47_001620"
     CDS             complement(19077..19640)
                     /gene="pabA"
                     /locus_tag="OQM47_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1479815.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19672..20274)
                     /locus_tag="OQM47_001621"
     CDS             complement(19672..20274)
                     /locus_tag="OQM47_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1479816.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20264..20431)
                     /locus_tag="OQM47_001622"
     CDS             complement(20264..20431)
                     /locus_tag="OQM47_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1479817.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20536..21117)
                     /gene="ppiA"
                     /locus_tag="OQM47_001623"
                     /gene_synonym="rotA"
     CDS             complement(20536..21117)
                     /gene="ppiA"
                     /locus_tag="OQM47_001623"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1479818.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21388..22569
                     /gene="tsgA"
                     /locus_tag="OQM47_001624"
     CDS             21388..22569
                     /gene="tsgA"
                     /locus_tag="OQM47_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1479819.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="OQM47_001625"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="OQM47_001625"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1479820.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="OQM47_001626"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="OQM47_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1479821.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="OQM47_001627"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="OQM47_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1479822.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cobA"
                     /locus_tag="OQM47_001628"
     CDS             26648..28021
                     /gene="cobA"
                     /locus_tag="OQM47_001628"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1479823.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28268..28435
                     /locus_tag="OQM47_001629"
     CDS             28268..28435
                     /locus_tag="OQM47_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1479824.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28730..30067
                     /gene="frlA"
                     /locus_tag="OQM47_001630"
                     /pseudo
     CDS             28730..30067
                     /gene="frlA"
                     /locus_tag="OQM47_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30088..31110
                     /gene="frlB"
                     /locus_tag="OQM47_001631"
     CDS             30088..31110
                     /gene="frlB"
                     /locus_tag="OQM47_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1479825.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31160..31990
                     /gene="frlC"
                     /locus_tag="OQM47_001632"
     CDS             31160..31990
                     /gene="frlC"
                     /locus_tag="OQM47_001632"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1479826.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31987..32772
                     /gene="frlD"
                     /locus_tag="OQM47_001633"
                     /pseudo
     CDS             31987..32772
                     /gene="frlD"
                     /locus_tag="OQM47_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32872..33603
                     /gene="phoB"
                     /locus_tag="OQM47_001634"
     CDS             32872..33603
                     /gene="phoB"
                     /locus_tag="OQM47_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1479827.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         8107 a         9008 c         8560 g         8040 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgttagtg
    12241 gtgtttcacg ggctggaagg cagtctcaat agcccttacg cccacggtct ggttgaggcg
    12301 gcgcaaaagc gcggctggct gggcgtggtg atgcattttc gcggatgcag cggtgaacca
    12361 aaccgtatgc accgcattta ccattcgggc gaaaccgaag acgccagttg gtttttacgc
    12421 tggctgcaac gcgaatttgg tcatgcgcca acggctgccg tcggctattc gctcggcggt
    12481 aatatgctgg cctgtttgct ggcaaaggaa ggcaatgatc tcccggttga tgcggcggtg
    12541 attgtctctg cgccgtttat gctggaagcc tgtagctatc atatggaaaa gggcttttcc
    12601 cgcgtttatc agcgttactt gctgaacctg ttaaaagcca atgccgcgcg caagctggca
    12661 gcctaccccg gaacgctgcc gattaatctc gcgcagttaa aatcggtacg tcgcatccgt
    12721 gaatttgacg atctaatcac cgccagaatt cacggctacg ccgacgctat cgactattat
    12781 cgtcagtgta gcgccatgcc aatgctgaac cggatcgcca aaccgacgct gattattcac
    12841 gccaaagacg atccgtttat ggatcatcag gtgatcccaa aaccggaaag tctcccctcg
    12901 caggtggagt atcaactgac tgaacatggc ggtcatgttg gctttattgg cggtacatta
    12961 cttcatccgc aaatgtggct ggagtcacgc attcctgact ggttaacaac gtatctggag
    13021 gcgaaatcat gttgattccg tggcaagacc tctcccccga aacgctggaa aatttaattg
    13081 aaagctttgt gttacgtgaa ggcaccgatt atggtgaaca tgagcgtaca cttgaacaga
    13141 aagtcgccga cgtcaaacgc cagctacagt gcggagaagc ggtgctggta tggtcggagc
    13201 tgcacgaaac ggtcaatatt atgccgcgca gtcagtttcg cgaataacct gcaaccgtcg
    13261 gcggacgaga taatagtgat agctaagtca tggagttact atgtctgcca aacatccggt
    13321 cattgcggta acaggatcca gcggcgcggg gaccaccacc accagcctcg cgtttcgtaa
    13381 aatattcgcg cagttaaatc tgcatgcagc tgaggtggaa ggcgacagtt ttcaccgtta
    13441 cacccgcccg gaaatggaca tggcgatccg caaagcgcgc gacgccgggc ggcatatcag
    13501 ctacttcggc cccgaagcga acgacttcgg cctgctggaa caaaccttca ttgaatacgg
    13561 tcagagcggc aaagggaaat ctcgcaaata tctgcatacc tacgacgaag ccgtaccctg
    13621 gaatcaggta ccgggaacat tcaccccctg gcaaccttta ccagaaccca ctgatgtact
    13681 gttttatgaa ggtttacacg gcggcgtagt cacgccacag cataacgttg cgcagcatgt
    13741 ggacttactg gtcggcgtag tgcctatcgt taaccttgag tggattcaaa aactgatccg
    13801 cgacaccagc gagcgcgggc actcacgaga agcagtgatg gactcggtag tgcgttcaat
    13861 ggaagactat atcaactaca tcacaccgca gttttcccgc acccatctta acttccagcg
    13921 cgttcccacc gtcgacactt caaacccgtt cgcggcaaaa ggtatcccgt cgctggatga
    13981 aagctttgtg gtgatccatt ttcgtaatct ggaagggatc gatttccctt ggctgctggc
    14041 gatgttgcaa ggctcattca tttcccacat caatacgtta gtggtacctg gcggcaaaat
    14101 ggggctggca atggaattaa ttatgctgcc gctggtgcaa cgattgatgg aaggaaagaa
    14161 aatcgagtaa ctttgctatt atgccggata aaatactatc cggcttcaca acgggatagt
    14221 taagtcacgc ggcaaccact tcatacgagt gagtaatatt caccgctttt tccagcatca
    14281 acgccactga acaatatttc tcggcagaga gatcaaccgc acgcgcaacc gctgcgtctt
    14341 tcaggtcgcg accggtgacg ataaaatgca gattaatgtg cgtaaacagg cgtggtgcct
    14401 cttcgcggcg ttcagaggtc aattttactt cacaatcgac cacatcctga cgcccttttt
    14461 gcaggatcga aaccacatcg atggcactgc aaccacccgc cgccatcaac accatttcca
    14521 tcggacttgg tgctttatcg cctgagttgc cgtccattaa aatctggtga ccagaggcgg
    14581 attcgcccag aaaagttaac ccttcgaccc acttcactcg cgcttgcatt tttgctactc
    14641 cactgcgtca attttcctga cagagtacgc gtactaacca aatcgcgcaa cggaaggcga
    14701 cctgggtcat gctgaagcga gacaccagga gacacaaagc gaaagctatg ctaaaacagt
    14761 caggatgcta cagtaataca ttgatgtact gcatgtatgc aaaggacgtc acattaccgt
    14821 gcagtacagt tgatagcccc ttcccaggta gcgggaagca tatttcggca atccagagac
    14881 agcggcgtta tctggctctg gagaaagctt ataacagagg ataaccgcgc atggtgcttg
    14941 gcaaaccgca aacagacccg actctcgaat ggttcttgtc tcattgccac attcataagt
    15001 acccatccaa gagcacgctt attcaccagg gtgaaaaagc ggaaacgctg tactacatcg
    15061 ttaaaggctc tgtggcagtg ctgatcaaag acgaagaggg taaagaaatg atcctctcct
    15121 atctgaatca gggtgatttt attggcgaac tgggcctgtt tgaagagggc caggaacgta
    15181 gcgcatgggt acgtgcgaaa accgcctgtg aagtggctga aatttcgtac aaaaaatttc
    15241 gccaattgat tcaggtaaac ccggacattc tgatgcgtct gtctgcacag atggcgcgtc
    15301 gtctgcaagt cacttcagag aaagtgggca acctggcgtt cctcgacgtg acgggccgca
    15361 ttgcacagac tctgctgaac ctggcaaaac aaccagatgc tatgactcac ccggacggta
    15421 tgcaaatcaa aattacccgt caggaaatcg gtcagattgt cggctgttct cgtgaaaccg
    15481 tgggacgcat tctgaagatg ctggaagatc agaacctgat ctccgcacac ggtaaaacca
    15541 tcgtcgttta cggcactcgt taatcccgtc ggagtggcgc gttacctggt agcgcgccat
    15601 tttgtttccc ccgatgtggc gcagactgat ttatcacccc gatatcaact atgcacttcg
    15661 acaaacgctg gtgctatgtt tgcccgtggc cgttgggtta atgcttggcg aattacgatt
    15721 cggtctgctc ttctccctcg ttcctgcctg ttgcaatatt gcgggccttg atacgcctca
    15781 taaacgtttt ttcaaacact taatcattgg tgcgtcgctg tttgccacct gtagcttact
    15841 gacacagctg ctactggtaa aagatgttcc cctgcccttt ttgctgaccg gattaacgct
    15901 ggtacttggc gtcactgctg agctggggcc attacacgca aaattgcttc ctgcatcgct
    15961 gctcgccgcc atttttaccc tcagtctggc gggatacatg ccggtctggg aaccattgct
    16021 catctatgcg ttgggcactc tctggtacgg attgtttaac tggttttggt tctggatctg
    16081 gcgcgaacaa ccgctgcgcg agtcactaag tctgctgtac cgtgaactgg cagattattg
    16141 tgaagccaaa tacagcctgc ttacccagca caccgatcct gaaaaagcgc tgccaccgct
    16201 gctggtgcgc cagcaaaaag cggtcgattt aattactcag tgctatcagc aaatgcatat
    16261 gctttccgcg caaaacaata ccgattacaa gcggatgctg cgtattttcc aggaggcgct
    16321 ggatttgcag gaacatattt cggtcagttt gcatcagccg gaagaggtgc aaaaactggt
    16381 cgagcgtagc catgcggaag aagttatccg ctggaatgcg caaaccgtcg ccgctcgcct
    16441 gcgcgtgctg gctgatgaca ttctttacca tcgcctgcca acgcgtttta cgatggaaaa
    16501 gcaaattggc gcactggaaa aaatcgcccg ccagcatccg gataatccgg ttgggcaatt
    16561 ctgctactgg catttcagcc gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc
    16621 ccgtgactta ctggccgata aacagcggcg aatgccgttg cttccggcac tgaaaagtta
    16681 tctgtcacta aagtctccgg cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt
    16741 tgccagcctg atgggcaccg tgctgcatct gccgaagtcg tactggatcc tgatgacggt
    16801 attgctggtg acacaaaatg gctatggcgc aacccgtctg aggattgtga atcgctccgt
    16861 gggaaccgtg gtcgggttaa tcattgcggg cgtagcgctg cactttaaaa ttcccgaaag
    16921 ttacaccctg acgttgatgc tgattaccac cctcgccagc tacctgatat tgcgcaaaaa
    16981 ctacggctgg gcaacggtcg gttttactat taccgcagtg tataccctgc aactgttgtg
    17041 gctgaacggt gagcagtaca tccttccacg tcttatcgat accattattg gttgtttgat
    17101 tgctttcggt ggtaccgtct ggctgtggcc gcagtggcag agcgggttat tgcgtaaaaa
    17161 cgcccatgat gctttagaag cctatcagga agcgattcgc ttgattctga gcgaggaccc
    17221 gcaaccaacg ccactggcct ggcagagaat gcgggtaaat caggcgcata acactctgta
    17281 taactcattg aatcaggcga tgcaggaacc ggcattcaac agccattatc tggcagatat
    17341 gaaactgtgg gtaacgcaca gccagtttat tgttgagcat attaatgcca tgaccacgct
    17401 ggcgcgggaa caccgggcat tgccacctga actggcacaa gagtatttac agtcttgtga
    17461 aatcgccatt cagcgttgtc agcagcgact ggagtatgac gaaccgggta gttctggcga
    17521 tgccaatatc atggatgcgc cggagatgca gccgcacgaa ggcgcggcag gtacgctgga
    17581 gcagcattta cagcgggtta ttggccatct gaacaccatg cacaccattt cgtcgatggc
    17641 atggcgtcag cgaccgcatc acgggatttg gctgagtcgc aagttgcggg attcgaaggc
    17701 gtaatacagg ctaaaccatc ggataaggca ttcacgccgc atccgacata ttttgcctga
    17761 tacgtcttat ttacgcccca accaccttcg ccaccgcgtg ggcgaaacgt tgcatccctt
    17821 catcgatatc cgcatcttcc accaccagcg acggtgctaa acgcatcaca tccggtccgg
    17881 cattcagcac cattacgcca acctctgcac ccgcatacag gaaatcacgc gcccgacctt
    17941 tgtagtgtgg tttcagctcc gcgccaatta acagccccat accgcgaata tcgctaaaca
    18001 catcgtactg ctgatcgatc ttctgcaaat ggtcaacaaa acgctggcgt ttcgcctgaa
    18061 tgccttccag cacttcaggg gtattgatga tatcaaacgc cgcgcccgct actgcacagg
    18121 ccagaggatt accgccgtag gtggaaccgt gagaaccagg atgaaacgca gaagcaattt
    18181 ccgccgtggt cagcatggcg ctaatcggga agccgccgcc taacgctttc gcagaagtca
    18241 gaatatccgg cgtcacaccg tagtacctgt aagcaaacaa atcgccagtc cgccccatcc
    18301 cgcactgcac ttcatcaaac accaataatg cctgatgttg atcgcacagc tcgcgcaagc
    18361 cctgcaaaaa ctctggcgtc gctgccgtca caccgccctc gccctggatc ggctcaacca
    18421 ccaccgcaca ggtgtgatca tccatcaccg ctttcactgc atggagatcg ttaaagggaa
    18481 cgtggatgat gtctgccggt ttcggcccaa agccgtcgga atattttggc tgcccaccca
    18541 ccgaaacggt aaacagcgag cgaccatgaa aagcgttatg gaaggcaata attttggttt
    18601 tgaacgggct atgacgcaca caggcgtaat ggcgtgccag tttaaaggcg gtttcgttag
    18661 cttccgtgcc ggagttcata aacaccacgc gttcggcaaa cgtcgcctca atcagtttgc
    18721 gcccaagacg cagcgccggt tcattggtga aaacgttact gatatgccac agagtttcgc
    18781 cctgggtttt taacgcgttc accagcgcag gatggcaatg gcccaacgcc gtaactgcaa
    18841 tgccacccgc gaaatcgaca tactccttgc cttgctgatc ccagattcgg ctgccctgac
    18901 cttttaccgg aataaactct gccggagcat aaatcggcag gatcacttca tcgaaagtcg
    18961 cgcgtgtaat tgctgtttgt tcaattgcca tctcatgatc accctgttac gcataaataa
    19021 atgtgaaatt ataatcacaa aatatgcata aaaaatcact gaatggcaat cggaaatcag
    19081 agatgcagga aattagccag cagttgatgt ccttgttcgc taagaatact ttctggatgg
    19141 aactgcacac cttccagatc ccactggcga tggcgaatcc ccataatctc gcgggtttcg
    19201 ctccaggccg tcacgtcaaa gcacgctggt aatgagtcag gttccaccac cagcgaatgg
    19261 tagcgtgtca cggtaagtgg atttgccagc ccccgaaata cgccctcacc gctatgtgta
    19321 atcggcgagg ttttgccgtg catgaccttt gcggcgcgca caactttgcc gccaaatgcc
    19381 tgcgccattg cctgatgacc gaggcagacg ccaagaatcg gcaagcgccc ggcatagtgg
    19441 cgaataacgt caagggagat cccggcttca tctggcgtac aggggccagg tgagatgaca
    19501 attttttgtg gtttaagggc gtcgatatcc gccagcgtca acgcatcgtt gcgcttaacc
    19561 agcacatccg cccccagttc acaaaagtac tggtagaggt tccaggtaaa agaatcgtag
    19621 ttatctataa gcaggatcat ggcggctccg gtacgaaaga accgcgctat tttactcaga
    19681 ttccccggct tcgcttacca ctttgctaaa tatcatctgc agtgcggtga gatcccccat
    19741 tgccccactc tgatttgcct gattccaggc ctctttttcg ataccctgcc agctcagttg
    19801 atatccggca tgaatcgcca gttgctcgaa gaagatccgc tgtgccagac cacttcccac
    19861 ccggaaggga tgcagcacgt tgatttcaca atagtaatgc gccagccgct cgacgaactt
    19921 cgctttctcc aggccaacca gataaccttc ttcctccaga tcctgcatca gggcattgcc
    19981 ctctttttcg atataagcaa agtggcagaa cggcgtatcg ccctgataaa tatcaacttc
    20041 acgcagttgc cctgcccagt cgaaaatatc ctggtacagc tggcgatgga tagtgcgcaa
    20101 atgcggtaaa ccgcgcacca gcggaccaag ctcaatggtc gcagcacgca gcgccgtcat
    20161 ttcgtaagcg gcctgttcca gccgctgctg ctggcggatg ttcagccggt tacgcatgat
    20221 atcaaggcca ggataaagat acagatcgcg cccttcgccg aatttatcgc tcatagtgcc
    20281 tcctcagctc ttcaaggcgc gctaaagcct ctgcggcagt aagagtgact aaaggcatct
    20341 cgacgccttc aagacggcga ctggcctgga aattacgatt acgctgaagc tcccagagac
    20401 gggacttttg cttatcggtg agtttcttca cttgatgcct ccctgaacgt gtccatttgc
    20461 cacaagtata agcggcaaat ccgggttacg ccgggagagc agaagcgcgg gcaagattgc
    20521 ccgcgcgaga aatcattacg gcaggacttt agcggaaagg ataactaccg gttttgacgg
    20581 cacattctgg tacggaccaa catcatgagt cggcacctgg gaaatcttat cggcaacgtc
    20641 catgcctttc accactttac caaataccgc gtaaccgaaa tcacgctgac catggtcaag
    20701 gaaggcgtta tcggcaacgt tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt
    20761 acgcgccatc gcgatggtgc cacgcgtgtt gcgcaggccg ttatcggctt catttttgat
    20821 tggcgggttt ggttttttct gctgcatctg ctcggtgaaa ccgccgccct gaatcataaa
    20881 gccaggaatg acgcggtgaa aggtagtgtt gttataaaaa ccgctgttca cataatcgac
    20941 aaagttttgc acagacactg gcgctttttg tttatccagc tccagttcga tgttaccagc
    21001 tgaggttgtc aacaatacgt gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc
    21061 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21121 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21181 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21241 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21301 acatttaaac agatcataaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21361 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21421 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21481 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21541 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21601 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21661 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21721 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21781 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21841 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21901 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacgt
    21961 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28201 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28261 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28321 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28381 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28441 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28501 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28561 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28621 acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28681 ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28741 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28801 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28861 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28921 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    28981 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29041 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29101 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29161 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29221 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29281 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29341 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29401 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29461 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29521 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggctctttgt
    29581 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29641 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29701 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29761 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29821 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29881 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    29941 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg ccatcgttat
    30001 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30061 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30121 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30181 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30241 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30301 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30361 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30421 ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg cgcggacagc
    30481 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30541 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30601 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30661 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30721 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30781 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30841 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30901 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    30961 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31021 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31081 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31141 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31201 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31261 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31321 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31381 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31441 ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31501 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31561 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31621 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31681 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31741 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31801 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31861 tattattgat cggggctatg agggttactc tacggtggag ctggtgacga tgtatatgaa
    31921 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    31981 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32041 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32101 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32161 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32221 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32281 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32341 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32401 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32461 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32521 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32581 attgcctagg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32641 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32701 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32761 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32821 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32881 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    32941 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33001 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33061 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33121 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc gacgaaagag
    33181 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33241 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33301 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33361 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33421 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33481 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33541 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33601 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33661 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggac
//
LOCUS       DAJVPT010000035        33693 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVPT010000035 DAJVPT010000000
VERSION     DAJVPT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            509..2794
                     /gene="nrdA"
                     /locus_tag="OQM47_001635"
     CDS             509..2794
                     /gene="nrdA"
                     /locus_tag="OQM47_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1479828.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3028..4158
                     /gene="nrdB"
                     /locus_tag="OQM47_001636"
     CDS             3028..4158
                     /gene="nrdB"
                     /locus_tag="OQM47_001636"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1479829.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4158..4412
                     /gene="yfaE"
                     /locus_tag="OQM47_001637"
     CDS             4158..4412
                     /gene="yfaE"
                     /locus_tag="OQM47_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1479830.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4466..5116)
                     /gene="inaA"
                     /locus_tag="OQM47_001638"
     CDS             complement(4466..5116)
                     /gene="inaA"
                     /locus_tag="OQM47_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1479831.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5295..5537
                     /locus_tag="OQM47_001639"
                     /pseudo
     CDS             5295..5537
                     /locus_tag="OQM47_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5579..6655)
                     /gene="glpQ"
                     /locus_tag="OQM47_001640"
     CDS             complement(5579..6655)
                     /gene="glpQ"
                     /locus_tag="OQM47_001640"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1479832.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6660..8018)
                     /gene="glpT"
                     /locus_tag="OQM47_001641"
     CDS             complement(6660..8018)
                     /gene="glpT"
                     /locus_tag="OQM47_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1479833.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8291..9919
                     /gene="glpA"
                     /locus_tag="OQM47_001642"
     CDS             8291..9919
                     /gene="glpA"
                     /locus_tag="OQM47_001642"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1479834.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9909..11168
                     /gene="glpB"
                     /locus_tag="OQM47_001643"
     CDS             9909..11168
                     /gene="glpB"
                     /locus_tag="OQM47_001643"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1479835.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11165..12355
                     /gene="glpC"
                     /locus_tag="OQM47_001644"
     CDS             11165..12355
                     /gene="glpC"
                     /locus_tag="OQM47_001644"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1479836.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12548..13498
                     /locus_tag="OQM47_001645"
     CDS             12548..13498
                     /locus_tag="OQM47_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1479837.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13539..14009)
                     /locus_tag="OQM47_001646"
     CDS             complement(13539..14009)
                     /locus_tag="OQM47_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1479838.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14064..15266)
                     /locus_tag="OQM47_001647"
     CDS             complement(14064..15266)
                     /locus_tag="OQM47_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1479839.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15353..15895)
                     /locus_tag="OQM47_001648"
     CDS             complement(15353..15895)
                     /locus_tag="OQM47_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479840.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16174..16599
                     /gene="nudI"
                     /locus_tag="OQM47_001649"
     CDS             16174..16599
                     /gene="nudI"
                     /locus_tag="OQM47_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1479841.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16638..17240)
                     /locus_tag="OQM47_001650"
     CDS             complement(16638..17240)
                     /locus_tag="OQM47_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1479842.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17548..18687
                     /gene="arnB"
                     /locus_tag="OQM47_001651"
     CDS             17548..18687
                     /gene="arnB"
                     /locus_tag="OQM47_001651"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1479843.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18691..19659
                     /gene="arnC"
                     /locus_tag="OQM47_001652"
     CDS             18691..19659
                     /gene="arnC"
                     /locus_tag="OQM47_001652"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1479844.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19659..21640
                     /gene="arnA"
                     /locus_tag="OQM47_001653"
                     /pseudo
     CDS             19659..21640
                     /gene="arnA"
                     /locus_tag="OQM47_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21637..22527
                     /locus_tag="OQM47_001654"
     CDS             21637..22527
                     /locus_tag="OQM47_001654"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1479845.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22527..24179
                     /gene="arnT"
                     /locus_tag="OQM47_001655"
     CDS             22527..24179
                     /gene="arnT"
                     /locus_tag="OQM47_001655"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1479846.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24176..24511
                     /gene="arnE"
                     /locus_tag="OQM47_001656"
     CDS             24176..24511
                     /gene="arnE"
                     /locus_tag="OQM47_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1479847.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24511..24897
                     /gene="arnF"
                     /locus_tag="OQM47_001657"
     CDS             24511..24897
                     /gene="arnF"
                     /locus_tag="OQM47_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1479848.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24891..25163)
                     /gene="pmrD"
                     /locus_tag="OQM47_001658"
     CDS             complement(24891..25163)
                     /gene="pmrD"
                     /locus_tag="OQM47_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1479849.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25268..26623)
                     /gene="menE"
                     /locus_tag="OQM47_001659"
     CDS             complement(25268..26623)
                     /gene="menE"
                     /locus_tag="OQM47_001659"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1479850.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26620..27582)
                     /gene="menC"
                     /locus_tag="OQM47_001660"
     CDS             complement(26620..27582)
                     /gene="menC"
                     /locus_tag="OQM47_001660"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1479851.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27582..28439)
                     /gene="menB"
                     /locus_tag="OQM47_001661"
     CDS             complement(27582..28439)
                     /gene="menB"
                     /locus_tag="OQM47_001661"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1479852.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28454..29212)
                     /gene="menH"
                     /locus_tag="OQM47_001662"
     CDS             complement(28454..29212)
                     /gene="menH"
                     /locus_tag="OQM47_001662"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1479853.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29209..30879)
                     /gene="menD"
                     /locus_tag="OQM47_001663"
     CDS             complement(29209..30879)
                     /gene="menD"
                     /locus_tag="OQM47_001663"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1479854.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30968..32263)
                     /gene="menF"
                     /locus_tag="OQM47_001664"
     CDS             complement(30968..32263)
                     /gene="menF"
                     /locus_tag="OQM47_001664"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1479855.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32342..32647)
                     /gene="elaB"
                     /locus_tag="OQM47_001665"
     CDS             complement(32342..32647)
                     /gene="elaB"
                     /locus_tag="OQM47_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1479856.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32702..33163)
                     /locus_tag="OQM47_001666"
     CDS             complement(32702..33163)
                     /locus_tag="OQM47_001666"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1479857.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33228..>33693
                     /locus_tag="OQM47_001667"
     CDS             33228..>33693
                     /locus_tag="OQM47_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCS1479858.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDK"
BASE COUNT         8179 a         9076 c         8389 g         8049 t
ORIGIN      
        1 aaagtaatga ataattttct tataatataa ggtaaattaa caaaatggct tcgcatttaa
       61 caataaccga atagaaaaca accatttcga catcaacaat cgcttacatt cgcttatata
      121 ttgaccacaa ctgatacatc agattatgtg atgactcgtg cttagatcaa tttttgcaat
      181 cattagcaaa aagattaata aaccatctat atcaatttat ccaacctatt ctgccgttca
      241 agaaatcgcc gaacagttat ttttaacaaa tttttctctt cccattgact ttcccggaca
      301 ccttgtctga cctaaggtgc gcgaaagcca ctttttcctt cctgagttat ccacaaagtt
      361 atgcacttgc aagagggtca ttttcacact atcttgcagt gaatcccaaa cataccccct
      421 atatatagtg ttctaagcag cttcccttac tacaggtagt ctgcatgaaa ctattgcgga
      481 aagaattcca aaaacaggta cgacatacat gaatcagaat ctgctggtga caaagcgcga
      541 cggtagcaca gagcgcatca atctcgacaa aatccatcgc gttctggatt gggcggcaga
      601 aggactgcat aacgtttcga tttcccaggt cgagctgcgc tcccacattc agttttatga
      661 cggtatcaag acctctgaca tccacgaaac tattatcaag gctgccgcag acctgatctc
      721 ccgtgatgcg ccggattatc agtatctcgc cgcgcgcctg gcgatcttcc acctgcgtaa
      781 aaaagcctac ggccagtttg agccgcctgc gctgtacgac cacgtggtga aaatggtcga
      841 gatgggcaaa tacgataatc atctgctgga agactacacg gaagaagagt tcaagcagat
      901 ggacacgttt atcgatcacg accgtgatat gaccttctct tatgctgccg ttaagcagct
      961 ggaaggcaaa tatctagtac agaaccgcgt gaccggcgaa atctacgaaa gcgcccagtt
     1021 cctttatatt cttgttgccg cgtgcctgtt ctcgaactac ccgcgtgaaa cgcgcctgca
     1081 atacgtgaag cgtttttatg acgcggtttc cacatttaaa atttcgctgc cgacgccaat
     1141 catgtccggc gtgcgtaccc cgactcgtca gttcagctcc tgcgtactga tcgagtgcgg
     1201 tgacagcctg gattccatca acgccacctc cagcgcgatt gtgaaatatg tttcccagcg
     1261 tgccgggatc ggcatcaacg ccggacgtat tcgtgcgctg ggcagcccga ttcgcggtgg
     1321 cgaagcgttc cataccggct gtatcccgtt ctacaaacat ttccagacag cggtgaaatc
     1381 ctgctctcag ggcggtgtgc gcggcggtgc ggcaacgctg ttctacccga tgtggcatct
     1441 ggaagtggaa agcctgctgg tgttgaaaaa caaccgtggg gttgaaggca accgcgtgcg
     1501 tcatatggac tacggggtac aaatcaacaa actgatgtat acccgcctgc tgaaaggtga
     1561 agatatcacc ctgttcagcc cgtccgacgt accggggctg tacgacgcgt tcttcgccga
     1621 tcaggaagag tttgaacgcc tgtataccaa atatgagaaa gacgacagca tccgcaagca
     1681 gcgtgtgaaa gccgttgagc tgttctcgct gatgatgcag gaacgtgcgt ctaccggtcg
     1741 tatctatatt cagaacgttg accactgtaa tacccatagt ccgtttgatc cgaccatcgc
     1801 gccagttcgc cagtctaacc tgtgcctaga aattgccctg ccgaccaaac cgctgaacga
     1861 cgtcaacgac gagaacggtg aaatcgcgct gtgtacgctg tctgctttca acctgggcgc
     1921 aattaatagc ctggatgaac tggaagagct ggcgattctg gcggttcgtg cacttgacgc
     1981 gctactggat tatcaggatt acccgatccc ggccgccaaa cgtggagcga tggggcgtcg
     2041 tacgctgggt attggtgtga ttaacttcgc ttactatctg gcgaagcacg gtaaacgcta
     2101 ctccgacggc agcgccaaca acctgacgca taaaaccttc gaagcgattc agtattacct
     2161 gctgaaagcg tctaatgagc tggctaaaga gcaaggcgcg tgcccgtggt ttaacgaaac
     2221 cacttacgcg aaagggatcc tgccgatcga tacctataag aaagatctgg atgccatcgc
     2281 taatgagccg ctgcattacg actgggaagc attgcgtgaa tcaatcaaaa cgcacggcct
     2341 gcgtaactcc acgctttctg ccctgatgcc gtccgagacc tcttcgcaga tctctaacgc
     2401 cactaacggt attgaaccgc cgcgcggtta cgtcagcatc aaagcgtcga aagacggtat
     2461 tttgcgccag gtggtgccgg actacgagca cctgcacgac gcctatgagc tgctgtggga
     2521 aatgccgggt aacgatggct acctgcaact ggtaggtatc atgcagaaat ttatcgatca
     2581 gtcgatctct gctaacacca actacgatcc gtcacgtttc ccgtcaggaa aagtgccgat
     2641 gcagcagttg ctgaaagacc tgctcaccgc ctacaaattc ggggtcaaaa cgctgtatta
     2701 tcagaacacc cgtgacggcg ctgaagacgc gcaggacgat ctggtaccgt caatccagga
     2761 cgatggctgc gaaagcggcg catgtaagat ctgatattga gatgccggat gcggcgtaaa
     2821 cgccttatcc ggcctacggc tcggtttgta ggcctgataa gacgcgccag cgtcgcatca
     2881 ggctccgggt gccggatgca gcgtgaacgc cttatccggc ctacggctcg gatttgtagg
     2941 cctgataaga cgcgccagcg tcgcatcagg cacaggatgc ggcgtaaaat gccttatccg
     3001 gcattaaact cccaacagga cacactcatg gcatatacca ccttttcaca gacgaaaaat
     3061 gatcagctca aagaaccgat gttctttggt cagccggtca acgtggctcg ctacgatcag
     3121 caaaaatatg acatcttcga aaagctgatc gaaaagcagc tctctttctt ctggcgtccg
     3181 gaagaagttg acgtctcccg cgaccgtata gattaccagg cgctgccgga gcacgaaaaa
     3241 cacatcttta tcagcaacct gaaatatcag acgctgctgg attccattca gggtcgtagc
     3301 ccgaacgtgg cgctattgcc gcttatttct attccggaac tggaaacctg ggtcgaaacc
     3361 tgggcgttct cagaaacgat tcattcccgt tcctatactc atatcattcg taatatcgtt
     3421 aacgatccgt ctgttgtgtt tgacgatatc gtcaccaacg agcagatcca gaaacgtgcg
     3481 gaagggatct ccagctatta cgatgaactg atcgaaatga ccagctactg gcaccttctg
     3541 ggcgaaggca cccacaccgt taacggtaaa accgtaaccg ttagcctgcg cgagctgaag
     3601 aaaaaactgt atctctgcct gatgagcgtt aacgcgctgg aagcgatccg cttctacgtc
     3661 agctttgctt gttcctttgc ctttgcagaa cgcgaactga tggaaggtaa cgccaagatt
     3721 attcgcctga ttgcccgcga tgaagccctg cacctgaccg gtactcagca tatgctgaat
     3781 ctgctgcgca gcggcgcgga tgatccggag atggcggaaa ttgccgaaga atgtaagcag
     3841 gagtgctatg acctgtttgt ccaggcggca cagcaagaga aagactgggc ggattatctg
     3901 ttccgtgacg gttcgatgat tggtctgaat aaagatattc tctgccagta cgttgaatac
     3961 atcaccaata tccgcatgca ggcagtcggt ttggatctgc cgttccagac gcgctccaac
     4021 ccgatcccgt ggatcaacac ctggctggtg tctgataacg tgcaggttgc tccgcaggaa
     4081 gtggaagtca gttcttatct ggtcgggcag attgacgcgg aagtggacac cgacgatttg
     4141 agtaacttcc agctctgatg gcccgcgtta ccctgcgcat cactggcaca caactgctgt
     4201 gccaggatga acacccttcc cttctggcgg cgctggaatc ccacaatgtg gcggttgagt
     4261 accagtgccg cgaaggttac tgcggctcct gtcgcacccg actggtcgca ggtcaggtgg
     4321 actggattgc cgaaccgtta gcctttattc agccggggga aattttgccc tgttgttgcc
     4381 gggcaaaagg cgatattgaa atcgagttgt gatcgggtac tagcgccaga tacgacgctt
     4441 acgcgtatta tctggcctac aacgattaca tggcgtagta atacgctttc acctgctccc
     4501 agtccgcttt agggatcggc tccagatatt tttccagctg gcggaagtca tgattaatcg
     4561 ctttatcgcg acgcaagcgg cgacgacttt tctccagatc aagaaaacca gcttctgcct
     4621 taccttctgt tttcacatag atatggcgaa catagcaaca gccatgctga cgattaacgc
     4681 tatgcatttt cttaaacgcc agtgccactg ctttcaacat ggcttgccgt acttcgtcag
     4741 aataaggcga tactgcatgc cgggcatacc agtcagcaat gctgatgaac cccgccatat
     4801 cttcagtcac cagcaacgcg cgccattcac cctcaatttt caccgcttca ccaaaaacga
     4861 tcttcggcac gatgacaccg gcccgttcca gttctttaat taccgcaacc tcacggacaa
     4921 tcgttggtcg gccgaacgga taacgtactg aatgaaacag atgatgcgtc atgcgcttta
     4981 catacagctt tttgccgttg cgctcgacgc attgcacccc gctcatacca ttacggcgat
     5041 agttaggctc ttcaacccag tcgccctctg ttgcccacca gtgattaaat tcgtcgtact
     5101 ttgctgaaac tgccataccc tatcgcctgt catttttatt aacgacaatg actataggtg
     5161 gttacctgag gaaaatctta atgaaacgtg tcgtattaat gaatttcttt tgccgctaca
     5221 ggtgtgacac cgcttttgcc cttcatttgc agcgcataga ttccaccagc cactaccact
     5281 aatagtgcgc ccagcagata aaatgtgaat ttaatcagtc ggatacagcg atgaatttta
     5341 ttcgacaggg attaggcatc gccttacaac cagagttaac gctgaaaagc attgcaggtg
     5401 aattgtgttc cgttcctcac gaaccaacct tctatcgaca gatttcgttg ctgactaaag
     5461 aaaagccggt agaaggcagt ccactgtttt tactacaaat gtgcatggaa caattagtgg
     5521 cgattggaaa aatttgatat gagacaggat ggcgcatgaa cgccatcctg cagtaacatt
     5581 actctttatt aagaaatttt actgccttat cagggaaatc agtaaacagc ccatttacac
     5641 ccgctttgtt atacagagca tcatataact gattcacatc aggagtgtat tcaggcagtt
     5701 tatctgaccg cacggtataa ggatgcacta ccagtttatt ctgctgggca tcttgcacca
     5761 tgccagtgag tttgatatta cccggctgcg atgtctcctc aatcaacata tggtaatccg
     5821 gaccaatacc atctgcatat tccgccacct gtttcatggc tcccggctta aacatccagt
     5881 cgtagttgta attaacccag cttccatccg gctgtttctg ctgcgtttca ttccagtcgg
     5941 tataggcaat cagctgtacc agattgagct ccatgcccat tttgggttcc agctcattct
     6001 taatacgctt cagctcatca gcatcaaaac attgcaaata aactttatcg tctttaccgg
     6061 tgtaaccata tttcttcagc acttccagcg tttttgccgc aatatccttc ccttcctgat
     6121 gatggaacca cggcgctttg atttctggat agataccgat atttttcccg gtagagtgat
     6181 ttaacccctg aacaaattca atctcttctt caaaggtgtg cacccggaag tcggacttac
     6241 ccattgggaa acgccccgga taagtctgta cttttttacc gttttcaata tcgaaacctt
     6301 cggtaaattt cagcgactta atttcatcca gcgtgaaatc tatcgcgtag taacgaccgt
     6361 ctttgcgcgc ccgatccggg aaacgatcgg caacatcagt aacacgatcg agataatggt
     6421 catgcagaac aaccagatgg tcgtctttgg tcatcaccaa atcctgttcc agataatccg
     6481 ctccctgcgc atacgccatc gcttttgctg gcagcgtatg ctccggcaaa tatccactgg
     6541 caccgcgatg ggcgatgact attttttcgt tgctgtccgc cgccattgca ctgcttccca
     6601 tgactatagt gctcatcatg atcgccatgc taaggttttt cagcttcaat ttcatgccat
     6661 tagcctccgt tgcgtttttg cagtaattgt tcatggcgac gtttttcgcc aatcatcaca
     6721 acaatcaaca agataaccgc cagaatgctg ccgccaatca ttaccataaa gccgccatcc
     6781 cagccgaaga agtccacggt gtagccaaca atcgcgctcg ccgccaccga accgcccagg
     6841 taaccaaaca gcccggtaaa gcccgctgcc gtacctgccg cttttttcgg tgccagttcc
     6901 agcgcatgca gaccgatcag catcacagga ccgtagatca ggaagccgat aacaatcata
     6961 caaatcatat cgacggttgg gttacctgcc gggttcatcc agtaaacgat agtcgcgatg
     7021 gtcaccagtg tcataaagaa gacgccggtt gccccacggt taccacggaa gactttgtcc
     7081 gacatccagc cgcacagcag ggtgcccgga atacccgcat actcatagaa gaagtaggcc
     7141 caggaggatt tatccagcgc gaaatgctta acctctttca gataagtcgg tgaccagtcg
     7201 aggatgccgt aacgcagcag ataaacgaac acgttggcga tggcgatgta ccacagcagt
     7261 ttgttcggca gcacgtactg catgaagatt tgcttcgcgg tcagctcctg ttccgctttt
     7321 tcgttatagt cgtccggata atcatttttg tactcttcga tcggcggcaa gccacaggat
     7381 tgcggggtat cgcgcatcat cgcaaaggcg aataatgcca ccagaatggc gcagaaagca
     7441 ggcatataga gcgccgcatg ccagtcattg aaccaggcca tccccagcag gaacagcagc
     7501 ggcggaatac caccaccgac gttgtgcgca cagttccaca ctgacacaat gccgccacgt
     7561 tctttctgcg accaccagtg caccatagta cgaccacacg gcggccaccc catcccctgg
     7621 aaccaaccgc agaggaacaa cagtacaaac atcaccgcaa tgctcgacgt cgcccatggc
     7681 acaaagccca taaacaacat cactgccgcc gccagaatca aacctgcggg caggaaaacg
     7741 cgcggattcg agcgatccga taccgaaccc atgatgaatt tcgaaaatcc ataagcaatc
     7801 gagatccccg aaagggcaaa acctaaatca ccgcgtgaga atccctgctc aaccagataa
     7861 ggcatagcaa gcgcaaagtt cttacgaacc aaatagtaag ccgcatagcc aaagaatatc
     7921 cccaggaaaa tttgccagcg caatcgacga taagtcggat cgatctccgc ggcaggtaag
     7981 cgcgctttgt gtggcgctgg tttaaaaata ctcaacattg atagcctccg tggcccgtgg
     8041 tcttatttat gattaacagc ctgattcagt gagagaacct gccgtttctt gagttgccgc
     8101 gatgttaaga aaacattcat aaattaaatg tgaattgccg cacacattat taaataagat
     8161 ttacaaaatg ttcaaaatga cgcatgaaat cacgttttac tttcgaatca tgagcgaata
     8221 tgcgcgaaat caaacaattc atgtttttac tatggctaaa tggtaaaaaa cgaacttcag
     8281 agggataaca atgaaaactc gcgactcgca atcaagtgac gtgattatca ttggcggcgg
     8341 cgcaacggga gccgggattg cccgcgactg tgccctgcgc gggctgcgcg tgattttggt
     8401 tgagcgccac gacatcgcaa ccggtgccac cgggcgtaac cacggcctgc tgcacagcgg
     8461 tgcgcgctat gcagtaaccg atgcggaatc ggcccgcgaa tgcattagtg aaaaccagat
     8521 cctgaaacgc attgcacgtc actgcgttga accaaccaac ggcctgttta tcaccctgcc
     8581 ggaagatgac ctctcattcc aggccacttt tattcgcgcc tgcgaagaag cagggatcag
     8641 cgcagaagct atagacccgc agcaagcgcg cattatcgaa cctgccgtta acccggcact
     8701 gattggcgcg gtgaaagttc cggatggcac cgttgatcca tttcgtctga ccgcagcaaa
     8761 catgctggat gccaaagaac acggtgccgt tatccttacc gctcatgaag tcacggggct
     8821 gattcgtgaa ggcgcgacgg tgtgcggtgt tcgtgtacgt aaccatctca ccggcgaaac
     8881 tcaggccctt catgcacctg tcgtggttaa tgccgctggg atctgggggc aacacattgc
     8941 cgaatatgcc gatctgcgca ttcgcatgtt cccggcgaaa ggatcgctgc tgatcatgga
     9001 tcaccgcatt aaccagcatg tgatcaaccg ctgccgtaaa ccttccgacg ccgatattct
     9061 ggtgcctggc gataccattt cgctgattgg taccacctct ttacgtattg attacaacga
     9121 gattgacgat aatcgagtga cggcagaaga ggttgatatt ctgctgcgtg aaggggaaaa
     9181 actggccccc gtgatggcga aaacgcgcat tttgcgggcc tattctggcg tgcgcccgct
     9241 ggttgccagc gatgacgacc cgagcggacg taacgtcagc cgtggcatcg tgctgctcga
     9301 ccatgctgaa cgcgatggtc tggacggatt tatcaccatc accggtggca aactgatgac
     9361 ctatcggctg atggctgaat gggctaccga cgcggtatgc cgcaaactgg gcaacacgcg
     9421 cccctgtacg actgccgatc tggcactgcc tggttcacaa gaacccgctg aagttacctt
     9481 gcgtaaagtc atctccctgc ctgccccgct gcgcggttct gcggtttatc gtcatggcga
     9541 tcgcacgcct gcctggctga gcgaaggccg tctacaccgt agcctggtat gtgagtgcga
     9601 agcggtaact gcgggtgaag tgcagtacgc ggtagaaaat ttaaacgtta atagcctgct
     9661 ggatttacgc cgtcgtaccc gtgtggggat gggcacctgc cagggcgaac tctgcgcctg
     9721 ccgcgctgcc ggactgctgc aacggtttaa cgtcacgacg tccgcgcaat ctatcgagca
     9781 actttccacc ttccttaacg aacgctggaa aggcgtgcaa cccatcgcct ggggagacgc
     9841 actgcgcgaa agcgaattta cccgctgggt ttatcaggga ctgtgtggtc tggagaagga
     9901 gcagaaagat gcgctttgat actgtcatta tgggcggcgg cctcgccgga ttactctgtg
     9961 gcctgcaact gcaaaaacac ggcctgcgct gtgccattgt cacgcgtggt caaagcgcgt
    10021 tgcatttctc atccggttcg ctggatttgc tgagccatct gccagatggt caacccgtgg
    10081 cagacattca cagtggactg gaatctttgc gtcagcaggc acctgcacat ccttactctc
    10141 ttctcgggcc acaacgcgtg ctcgatctcg cttgccaggc gcaggcatta atcgctgaaa
    10201 gcggtgcgca attgcagggc agcgtagaac tggctcacca gcggattacg ccgctcggca
    10261 ctttgcgtgc aacctggtta agttcgccag aagttcccgt ctggccgcta cccgcgaaga
    10321 agatatgtgt agtggggata agcgggctga tggatttcca ggcgcatctt gcggcggctt
    10381 cgttgcgcga actggatctt tcggttgaaa ccgcagaaat agagctgccg gaactggatg
    10441 tcctgcgcaa taacgccacc gaatttcgcg cggtgaatat cgcccgtttc cttgataatg
    10501 aagaaaactg gccgctgtta cttgatgcgc ttattcctgt cgccaatacc tgcgaaatga
    10561 tcctgatgcc cgcctgcttc ggcctggccg atgacaaact gtggcgttgg ttgaatgaaa
    10621 aactaccttg ttcactgatg cttttgccaa cgctgccgcc ttccgtgctg ggcattcgtc
    10681 tgcaaaacca gttacagcgc cagtttgtgc gccaaggtgg agtgtggatg ccgggcgatg
    10741 aagtgaaaaa agtgacctgt aaaaatggcg tagtgaatga aatctggacc cgcaatcacg
    10801 ccgatattcc gctacgtcca cgtttcgcgg ttctcgccag cggcagtttc tttagtggcg
    10861 gactagtagc ggaacgtaac ggcattcgcg agccgattct cggccttgat gtgctacaaa
    10921 ccgccacgcg gggtgaatgg tataagggcg atttttttgc gccgcaaccg tggcagcagt
    10981 tcggtgtaac cactgatgag acgctacgcc cgtcgcaggc agggcaaacc attgagaacc
    11041 tgtttgccat cggttcggtg ctgggcggat ttgatcccat cgcccaggga tgcggcggcg
    11101 gtgtttgtgc cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc gcaggaggcc
    11161 aacaatgaac gacaccagct tcgaaaactg cattaagtgc accgtctgca ccaccgcctg
    11221 cccggtgagc cgggtgaatc ccggttatcc agggccaaaa caagccgggc cggatggcga
    11281 gcgtctgcgt ttgaaagatg gcgcactgta tgacgaggcg ctgaaatatt gcatcaactg
    11341 caaacgttgt gaagtcgcct gcccgtccga tgtgaagatt ggcgatatta tccagcgcgc
    11401 gcgggcgaaa tatgacacca cgcgcccgtc gctgcgtaat tttgtgttga gtcataccga
    11461 cctgatgggt agcgtttcca cgccgttcgc accaatcgtc aacaccgcta cctcgctgaa
    11521 accggtgcgg cagctgcttg atgcggcgtt aaaaatcgat catcgtcgca cgctaccgaa
    11581 atactccttc ggcacgttcc gtcgctggta tcgcagcata gcggctcagc aagcacaata
    11641 taaagaccag gtcgctttct ttcacggctg cttcgttaac tacaaccatc cgcagttagg
    11701 taaagattta attaaagtgc tcaacgcaat gggtaccggt gtacaactgc tcagcaaaga
    11761 aaaatgctgc ggcgtaccgc taatcgccaa cggctttacc gataaagcac gcaaacaggc
    11821 aattacgaat gtagagtcga tccgcgaagc tgtgggagta aaaggcattc cggtgattgc
    11881 cacctcctca acctgtacat ttgccctgcg cgacgaatac ccggaagtgc tgaatgtcga
    11941 caacaaaggc ttgcgcgatc atatcgaact ggcaacccgc tggctgtggc gcaagctgga
    12001 cgaaggcaaa acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc acactccgtg
    12061 ccatatggaa aaaatgggct ggacgctcta caccctggag ctgttgcgta acatcccggg
    12121 gcttgagtta acggtgctgg attcccagtg ctgcggtatt gcgggtactt acggtttcaa
    12181 aaaagagaac taccccacct cacaagccat cggcgcacca ctgttccgcc agatagaaga
    12241 aagcggcgca gatctggtgg tcaccgactg cgaaacctgt aaatggcaga ttgagatgtc
    12301 cacaagtctt cgctgcgaac atccgattac gctactggcc caggcgctgg cttaaactcc
    12361 cttctgatgc ccggtaagca tgtggttacc gggcattttt gcgtacacga ttccgtgccc
    12421 aatgtatgcg ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa ggctgaaact
    12481 ggcagatgtc aaaggcctgg gataaccgta atgtcgcgtc atcataaata tcaggtgacg
    12541 gacaaccatg accgaatcaa caacctcctc cccgcatgat gcggtattta aaacctttat
    12601 attcacaccc gaaaccgcac gggattttct cgaaatacat ttaccagaac cactgcgcaa
    12661 gctttgcaac ctgcaaacct tacgcctgga acccactagt tttattgaaa aaagtttacg
    12721 cgcttactac tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg gctatatcta
    12781 ctgcgtgatt gaacatcaaa gctctgcaga aaagaatatg gcttttcggc caatgcgcta
    12841 tgccactgcc gccatgcaga gtcacctaga taaaggctat gacagagttc cgctggtggt
    12901 gccattgctg ttttatcatg gcgaaacatc gccctacccg tactcactta actggctgga
    12961 tgagtttgac gatccgcaac ttgcccggca gttgtacacc gaagcttttc cgttggtgga
    13021 tatcaccatc gtacctgacg atgagatcat gcaacatcgg cgtatagctc tgctggaact
    13081 gattcaaaag catattcgcg accacgattt aatcggcatg gtcgacagga tcaccacgct
    13141 tttggttaga ggcttcacta atgacagcca gctacaaaca ctgtttaatt atctgctgca
    13201 atgcggcgat acctcccgtt tcacccgttt tattcaggag attgccgaac gttcaccact
    13261 acaaaaggag agattaatga ctattgctga acggctacgg caggaaggac atcaaattgg
    13321 ctggcaagaa ggtaaattag taggtttgca gcaaggtaaa ttagaaggtt tgcaggaagg
    13381 tatgcatgaa caagccatta aaattgcttt gcgcatgctg gaacagggca ttgatcgtga
    13441 ccaagtgctc gcggccaccc agctaagcga agccgatctg gcagcgaata accactaatt
    13501 aacacaggcc cacagccgat ccccatgggc ctttgatatc aataactacc ttttatgcgt
    13561 ggcccatttt tgcctgattt aaacatcgcc agtcgttgat ccagggcatc gctgtagagc
    13621 atcgtgtcaa cgccaacagc gacaaagttc gctccccacg ccaggcattg ctgcgccata
    13681 tcaggagcca cagccagaaa accagccgct ttacccgcag cacggatccg ccgaatgctg
    13741 gtttcaataa ttcgctgcac ttccgggtgc ccggcgttat ccgggtagcc caacgacgca
    13801 gaaagatccg ctggtccaat aaacacgcca tcaatccctt cgacgtcgag gatctcgtcc
    13861 aggttatcca gtgccgtttt actttccacc tgcactaaca gacaaagcga atcgttgact
    13921 tgcgccatgt aattctcaat gcgtccccag cgcgcagccc gtgccacact ggccccaaca
    13981 ccacgctcac cgtagggagg gttggacaaa ttttgccgga gggggaatgt gccggtgcag
    14041 taaaccggca cagagtggcg aaatcagacg gataaagtca tggactcaac aacctcaatc
    14101 cagccatgct cactagcgat gtcctggccg tttaaccaac ggcgtagcat attcagtgcc
    14161 atcattgcgc acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca
    14221 cgcagagcga aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa
    14281 cccgaaacag ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc
    14341 gcagtttgcg ccagggtttc ttcctgtgaa ggaatcactt cacacgccag caatggagca
    14401 cctgcgcgag aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg
    14461 ctgaactggc ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg
    14521 aaaatcacgc tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt
    14581 tgctcgcttg ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc
    14641 attgttacgc ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa
    14701 cgaccaaagg tagttaaacg cagacaaacc ggcggctgtg gtaaagaaaa acgctcgcgc
    14761 aggcgcggca ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta
    14821 aagaacatca ggcaacgatt aagctgcacg gcaaaaccac aggcggtgcc taccgggtta
    14881 ttgataaatt cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact
    14941 cgtccacgtt cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg
    15001 ccttcacctt ttgctgtcgc agcggcgagt gcgcttaaat catcgctggt cggtcccagc
    15061 ccgccgttaa cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag
    15121 tcatcaaggt tatcccccac cgtattgcgg cgagataatg gcagcccctg atgaaagaaa
    15181 aaatcggcca gccaggcagc gttagtgtca acgatttgcc cgtataacac ttcatccccg
    15241 gtggatagca tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata
    15301 acgcaaaggg agataaggcg tagtaagaaa ctgaatgccg cgccggaagg gatcagaaac
    15361 tggcgttaac cccaacatac aggccgtcag ccacagcgtt gtcgcggtta ccgtctttac
    15421 ccgacagatt caggtagcga taacccgcct caatactgac tggacgcata atggtgtaac
    15481 gcgcgccagc attcgcttcc tcataacttt gaataccgct ggagagcgaa tccggagagt
    15541 agtaatactc gccaaacaaa cggaagctgt tgccaatttt ccactgcaaa ccacctccta
    15601 ccgctgcggc ataaccttca tcgccgtaat tcgggttggt gtacacgcct tttccgccaa
    15661 cggtcgccat taacggcccg agaggcagat tcaaccccag ccccacgccc gcgacgtcac
    15721 cgtcgtcgtc gttatgtgtc cagttaccgc ttaaagccag gcccgtcgat tcagtcccaa
    15781 aaccgacacc aatattggtg tattctttac ccgcctggcc gctgatgctc attgcattga
    15841 ccgatgcaga aaccaacagc attccggcca gacctgctag cgcaattttt ttcattatcc
    15901 cctttcccac aagcaattaa tcaaaattcc caaagctgcg ggattgtact gcctggcaca
    15961 gaggattcaa tgagcgaaaa aagggatcta gtcacttatt gtaactaaat acttcttaaa
    16021 taattaaacg ccgaaaaaat aaacacactt taccgataca cacaaaaata ataataagtg
    16081 cagttatcat tgcaaaagag attactgtca ctttcacata tttctgagtt accgtattct
    16141 catggtttct ttttatttat aaggaatacc cctgtgcgac aacggactat tgtatgccct
    16201 ttgattcaaa atgatggtgc ttatttgctg tgcaaaatgg ccgacgatcg cggcgttttc
    16261 cccggtcaat gggcgctttc gggtggcggc gtggagtctg gcgaacgaat tgaagaggca
    16321 ctacgccgcg aaattcgcga agaactggga gaacagctgc ttttgacaga aatcacgccg
    16381 tggaccttca gcgatgatat tcgcaccaag acatatgcag atggtcgcaa ggaagagatt
    16441 tatatgattt acctgatttt tgactgcgtt tctgccaacc gagaagtgaa aataaacgaa
    16501 gagtttcagg actacgcgtg ggtaaaacct gaagatctgg tgcattatga tttaaatgtt
    16561 gccacccgaa aaacgttacg tttgaaaggt cttctttaac aatgatgaca gccatcactg
    16621 gctgtcagat tgaaaattta gtggttaacg aattccccat ccagataaac tttgcctttt
    16681 tccacatgca tgactaaacc atccagataa tcaggtttaa atatcgcatc acgcttattt
    16741 ttagcaatat atgtcaggca atgattatgg gtgaaaataa cgatattttt atcaggcgct
    16801 ttgttttgta agtccttaat tgcactataa atctcattac cgcactgaag aagtcgttta
    16861 tctaccgtca gctttttacc cgcggaaaac caggtcgccg actgaatggt ccggacggta
    16921 ttactggaat aaagatcgaa atcagagatg tcagcgctaa aagcgttgcc cagttcacgg
    16981 gcatcctggg tacctttaac cgtaatacct gttttatctg acaagcattg attggttgaa
    17041 cgatcgcaac gttcagcatg gcgaaacaaa acgacaaccg ggtgctgctg tgccagtctg
    17101 gccaatgttt tattgtcgat acgtggcaaa ccattgctac tccaggcggc atgagtaccc
    17161 agtccggcaa ttgcagcgag cgccagtaaa atgatgaaat atttttttga cttcaatgat
    17221 gagcggcaaa aagctaacac gtaaactcca cctatagaca agcgcaacca gacaattacc
    17281 gtgaaattga gcgacatttc tggcgataat tagcagttgg tgtaatatta aaaatcctac
    17341 gatgtcggca aaatgcctca aatttttgcc aaatgcaaag cctaaataag aaaaaatata
    17401 aaaatttcaa tatttacgtc taatattagt ttcttaaggt taagttaata ttctatcctt
    17461 aaaatttcgc tccaaatggc aaaatataca caacactctt tatagcaaat ataagtggac
    17521 aggtattcaa tggcggaagg aaaagcaatg tcagaatttt tgcctttttc gcgaccagca
    17581 atgggcgtgg aggaactcgc tgcagttaaa gaggttctcg aatccggttg gatcacaacc
    17641 ggtccgaaga atcaggcgct tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc
    17701 atcgcggtca gttcagccac cgccggaatg catatcacgc taatggcgtt ggaaattggc
    17761 aagggcgatg aagtgattac gccttccctg acctgggttt caaccctcaa tatgatttcc
    17821 ttgctgggtg caacgccggt aatggtggat gtcgaccgcg atacgctgat ggtcacgcct
    17881 gaagcgatcg aatcagccat tacgccacgc actaaagcca tcattccggt gcattatgcc
    17941 ggtgcgccag cagatattga cgccattcgc gccattggcg aacgttacgg catcgcagtt
    18001 atcgaagatg ctgcccatgc cgtcggtacg tattacaaag ggcgacatat tggcgcaaaa
    18061 ggtaccgcta ttttttcatt tcatgccatt aaaaatatta cctgtgctga aggtggcctg
    18121 attgtaactg ataatgaaaa ccttgcccgc cagctacgga tgctgaaatt tcacggtctg
    18181 ggtgtcgatg cctatgacag acaaacctgg ggccgtgcac cgcaggctga agtcttaaca
    18241 ccgggctata agtacaatct gaccgatatt aacgccgcga ttgccctgac acagttagtc
    18301 aaattagagc acctcaacac ccgtcggcgc gaaattgccc agcaatatca gcaagcactg
    18361 gcagctctcc cctttcagcc attaagcctt cccgcctggc cgcacgttca cgcctggcat
    18421 ctgtttatta ttcgtgtcga tgaacaacgt tgtggtatca gtcgcgatgc gttgatggaa
    18481 gcgttaaaag aaagaggcat tggtaccggg ttacatttcc gcgccgctca cacacaaaaa
    18541 tattatcgcg agcgttttcc cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc
    18601 atctgttcgt tgccgctgtt cccggatatg actaccgccg atgccgaccg cgtcatcaca
    18661 gcccttcagc aactcgcagg acaataagcc atgtttgaaa tccaccctgt taagaaagtc
    18721 tcggtggtta ttcccgttta taacgagcag gaaagcttac cggaattaat caggcgcacc
    18781 accacagcct gtgaatcgtt ggggaaagag tatgagatcc tgctgattga tgacggcagt
    18841 agcgataatt ccgcgcatat gctggtcgaa gcctcacaag cggagaacag ccatattgtg
    18901 tctattttga ttaaccgcaa ttacgggcaa cattcagcga ttatggcggg attcagtcac
    18961 gttactggcg acttaattat tacccttgat gccgatctcc agaatccgcc agaagaaatc
    19021 ccccgcctgg tggcaaaagc cgatgaaggt tacgacgtgg tagggactgt acgccagaac
    19081 cgccaggaca gctggtttcg taaaacagct tcgaagatga ttaaccggct tattcagcgc
    19141 accactggca aagcgatggg tgactacggt tgtatgctgc gcgcctatcg ccgtcatatt
    19201 gtcgatgcga tgttgcactg ccatgaacgc agcaccttta tcccgattct ggcgaatatc
    19261 ttcgcccgcc gtgcaattga aattccagta catcatgccg agcgtgagtt tggtgaatcc
    19321 aaatacagtt ttatgcgcct gattaatttg atgtacgacc tggtgacctg ccttaccaca
    19381 acgccgctac gtatgctgag tctgctcggc agcattattg cgattggagg ttttagcatt
    19441 gcggtgctgc tggtgatttt acgcctgacc ttcggaccac aatgggcggc agaaggcgtc
    19501 tttatgctat ttgccgtgct gtttactttt attggcgctc agtttatcgg catgggatta
    19561 ctcggtgaat atatcggcag gatctacacc gatgtccgcg cccgcccccg ctattttgtt
    19621 cagcaagtta tccgtccatc cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct
    19681 accacgatat gggatgcctc ggtattgaag ctctgctggc tgccggttac gaaattagcg
    19741 ccatttttac ccataccgat aatcccggtg aaaaagcctt ttatggttcg gtggctcatc
    19801 tggcggcgga aagagacatt ccggtttatg cgccggataa cgttaatcat ccgctgtggg
    19861 tggaacgcat tgcccaactg tcaccagagg tgattttctc tttttattat cgccatctta
    19921 tttgcgacga gatttttcag ctcgctcccg caggtgcatt taatctgcac ggttcgctgt
    19981 taccaaaata tcgtggtcgc gcgccgctga actgggtgct ggtgaacggt gaaacggaaa
    20041 cgggcgttac attgcaccga atggtaaacg tgccgatgct ggggccattg tggcgcaact
    20101 gcgcgttgcc attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc
    20161 cgcgcgccag ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat
    20221 cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct
    20281 tgaatggcat aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg
    20341 gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca
    20401 tcctcatgcc agcaaagcac agccggggag cgtgatttct gttgcgccac tgctgattgc
    20461 ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca
    20521 gggctcgcaa ttagcgcaga tgctgggcct ggtgcaaggt tcacgcttga atagccagcc
    20581 tgcctgcacc gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg
    20641 caaccatctg acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat
    20701 tggcagcgat gcgataagcc gttttctgaa tcatccacat tttcactttg tcgaaggcga
    20761 tatcagtatt cattccgaat ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc
    20821 gctggtggcg atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact
    20881 cgattttgaa gagaatctgc gcattatccg ctactgcgtt aagtaccgta agcgaatcat
    20941 cttcccgtca acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca
    21001 ttctaattta atcgtcggcc cggtgaataa accacgctgg atttattcgg tgtcaaaaca
    21061 attacttgat cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt
    21121 cctcccgttt aactggatgg gaccacgact ggataacctt aatgcagcgc gaattggcag
    21181 ctcccgcgct attacgcaac tcattctcaa tctggtagaa ggttcaccaa ttaagctaat
    21241 tgatggcgga aaacaaaaac gctgctttac tgatatccgg gacggtatcg aggcgttgta
    21301 ccacattatc gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc
    21361 tgagaacgaa gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca
    21421 tccgctgcgc catcatttcc caccgtttgc gggctttcgc gttgtcgaaa gtagctgcta
    21481 ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccaccg
    21541 ctgcctggac tgggagccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt
    21601 cttcctgcgc accgttgatc ttacggataa accatcatga ccaaagtagg cttacgcatt
    21661 gatgtcgatg cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt
    21721 aagcataata ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat
    21781 ctctggcgac tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg
    21841 ctttatggct gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat
    21901 gccaatgccg atatcattcg tgaagcggct aaacatcacg aagtcggtct gcacgcctgg
    21961 gatcaccatg cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat
    22021 attgcacgcg gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc
    22081 gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc
    22141 tacaacagcg attgtcgtgg ggccataccg ttccgtccac tgcttgaatc aggaaaccct
    22201 ggcactgcgc aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg
    22261 aaagcagaag attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg
    22321 gtttatacca ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc
    22381 ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag
    22441 acgttgccgc tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt
    22501 tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcatcttcg
    22561 cgtttattgc ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg
    22621 aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg attgtgcccc
    22681 atctgttagg gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg
    22741 ggcaatggct atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc
    22801 tgttaactgc cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg
    22861 ctctactcgc cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg
    22921 ccgtgctcga tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg
    22981 caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct
    23041 gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc
    23101 tgccatgggt agcgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg
    23161 ttatcagttg cgtactgacg gttctcccct ggggactggc gatagcgcag cgggagcctg
    23221 acttctggca ctattttttc tgggttgagc atattcaacg ctttgcacta gatgatgccc
    23281 aacatagagc tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc
    23341 tgggattact ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg
    23401 tctatttgtt gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggcaaat
    23461 tgcccaccta tattctttct tgctttgcac ctctggcaat gctgttggcg cattacgcgt
    23521 tgctggcagc aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt
    23581 ttggcgtcac tggcattatt gccacatttg tgatctcccc ctggggacca atgaacacgc
    23641 cggtgtggca aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat
    23701 gggcattttt cggctggtac accttaacaa acgtcgaaaa gacctggtct ttcgccgcgc
    23761 tttgcccgct ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag
    23821 gaaaacatcc gcaatttttt gtcgagatga cacaagaatc acttcagcca agccgctata
    23881 ttcttactga tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca
    23941 tcatcatgta tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag
    24001 ggagatttgt cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga
    24061 ttattacact cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc
    24121 ccgcagatgc catcgttcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat
    24181 ctggctaaca ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca
    24241 ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact
    24301 ggcgctggct tgtcttggtc ttgccatgat gctctggctg ctggtcttgc agaacgtacc
    24361 ggtaggcatt gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt
    24421 aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg
    24481 tggcattgtg atcctcggga gtacggtgta atgtgcctga tatggggatt attcagcgtc
    24541 attattgcct cagttgcgca attaagcctg ggttttgcgg caagtcatct gccgccgatg
    24601 acgcacttat gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg
    24661 ttactcgggc tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa
    24721 cttgcgctaa gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct
    24781 tcaatggtac ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc
    24841 tgtattatga gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt
    24901 ttccctgccg ctgtacactg ccgttcccac tcatcggggc tataactgct tgccgagagg
    24961 actttcaccg tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag
    25021 agtaaatccc ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg
    25081 ccgccagcat cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt
    25141 ttgtgccagc cattccattg cattatcctg tttgctaaga gttttcacat caatttacag
    25201 attaacagat aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga
    25261 gggttaatta ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac
    25321 cgccgttttt cagctccggc ggtaaagtca gccagcgcac cggttgttga aaacgagcca
    25381 gcttatcttt cacccattcg ccaagatcaa cggtttgctg gtcatactcc accaccgcta
    25441 ccggccgatg accaaactcc ttgtcggcaa cggaaacgat aaacacctgc aaaaccgcag
    25501 gatgtgcagc aataacacgc tcgacttctt ccggctgaat accctcaccg ccactgaaga
    25561 acagattgtc taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc
    25621 gcgtagcgta ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac
    25681 cttctgccat actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac
    25741 ccggcagcgg caaaccaacg tctgccaggc cgtcggcttc tttcgcacat accgtggagg
    25801 caaactcggt cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg
    25861 tcaactcgac cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc
    25921 ggttaaccag caaacgccag agttgtgttg gcaccagtga agcgtgagta cagcctgcca
    25981 gcatttgctc caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc
    26041 gccacataat tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt
    26101 gatcgccaaa cggaatcagc gataacacac cttgcgcact ggcaagatgg gcctgataag
    26161 tatggacagc ggctttcggc aaaccggtag aacccgaggt caacgtcatt gagcacagac
    26221 gcgtcggctg ccacgcagcg gcatgtacgc cttcaaccag ctgaatgtgc agcgacgtta
    26281 atgccggaaa cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca
    26341 gcaattcttc aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc
    26401 attgcagtaa cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca
    26461 cgccgctgcc ctcaaccacc ccctgcgccg caaagcccgt agctaactca tcgacgcgag
    26521 cacaaagctc gcgccagttg agttgctcgt cattaagacg taaggcgatg gcttctcccc
    26581 gcacttgccg ccagtgacgc cacggccagt cagagaagat cataacaact gctcctgtac
    26641 atcagcgtcc actaacggca gtgggctccc cggccagcga cgaacctgct gcgcctgcat
    26701 cagatccagc gtgtccagtc ccggaatggt gtctggcgtt aaccaggcag caatccgcgc
    26761 cagttgcgtt agacctaagc tcgactcaat ggaagaactg atcaccgccg tcagccccag
    26821 cgcatgcgcc gcctgtacct gctcgcgtac tttttccaga ctgcccgtga gcgtgggttt
    26881 gataactacc gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaggct
    26941 ttcatcccag gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt
    27001 tttgcacggc tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc
    27061 aaactgctga cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc
    27121 cggaattgcc tccagcaaca gattcaccac catgccgtcg cgcaccgctt cgtacaatcc
    27181 cactttgacc ttcgccactt tctcgcccgg catatcggca agtttgagga tcagatcgtc
    27241 cggatcgcca ttacacagcg gtgccgcacg gtagttggct gcttgcggca acgtatctgt
    27301 cagttctgcc aatgcacagc ttacgccaaa ggccacggaa ggcatctgcg gtagctcgca
    27361 atcgcctgcc agccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccagtt
    27421 ttcctggctg aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg
    27481 caggcaaacg tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc
    27541 gtccatgggg atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat
    27601 ttgctgaaat caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc
    27661 gtcatgtaga acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca
    27721 cagtcggcgt tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt
    27781 tcgcggcacc aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca
    27841 aggcccatat ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc
    27901 gcttttttct gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa
    27961 ccgactttcg ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac
    28021 atcatgtgca gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt
    28081 ttcggacagg tacggatctg acgctggaag tccagcacgt tcaggtgatg tacgccggaa
    28141 tcatctttat agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct
    28201 ttatcgcctg cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc
    28261 agtgcctgga tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga
    28321 ttaatggtga tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct
    28381 tcggagcagt cgtgccattc aaccggtgcg taaggcattg cttcatcagg ataaatcata
    28441 ttgtgtcctt tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat
    28501 tttcccgatg cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg
    28561 ccgccagggc gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc
    28621 gggcgctaag gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg
    28681 cggcaagggt tgcgccatta ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt
    28741 cattgagcga ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt
    28801 ctgtgcgaaa acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag
    28861 cattttgcag ccccggatgc ccgccttcga caataacccc acaaagcccc gccagcccct
    28921 ggcaagccgc catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa
    28981 ggatgttgta actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga
    29041 cgctaatcgc cgccgaacca ccatgaccta ggagatcaac atacaaccgt gagtagtcgg
    29101 caaacgcctc gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca
    29161 ccagccaggg taaacctagt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct
    29221 tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc
    29281 aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca
    29341 gttttgcgga tgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt
    29401 ttgcggcatc agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat
    29461 ttgcccgccg ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa
    29521 cgccagcgcg ttgagatcgt aaagcgcgga gagatcgccc acaatcgcca gcgtcggttt
    29581 gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc
    29641 tcggttgctg tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac
    29701 caccaggctg ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg
    29761 cgccagttgc gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc
    29821 cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag
    29881 ccagtcggca atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc
    29941 ttcaatgtca tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag
    30001 ccgtttcccc gtcaggctac ttcccagttg caccacaatt tgcgcctgct gcagctcgct
    30061 ggtcgctttg gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga
    30121 cagcacatcg ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc
    30181 ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca
    30241 gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg
    30301 ccaccagtca cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag
    30361 cggttcagca aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg
    30421 gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat
    30481 actgtgcgtg gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca
    30541 gtcaattagc tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc
    30601 ggcttcaatc agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc
    30661 caccggctgc ttgctgactt tcgccagccc cagcgccaga tgccccaacc cacgctcatc
    30721 gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga
    30781 acgcgagcct ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat
    30841 gaccgccgcc cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat
    30901 tgcggctaag tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga
    30961 ctcattacta ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct
    31021 gccactcttg ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga
    31081 cattgccgct aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga
    31141 gatagcccgc agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact
    31201 gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac
    31261 agatcacatc atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt
    31321 tacgcagacg cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc
    31381 gttggcagat atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca
    31441 gccactctcc taactgctgc gcctgcttat catcaggatg atttgctact gttcccgcca
    31501 gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa
    31561 gaaaagcatt ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac
    31621 gactggcagc catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc
    31681 gagcgagcac cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta
    31741 attgcgtcca gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc
    31801 caggcaaggg cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt
    31861 gctgaaggga gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc
    31921 gccattccag gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc
    31981 aaatgcgtaa gtcagcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg
    32041 acgtaaaacg ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc
    32101 agtagaattg cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt
    32161 tgagagggaa aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca
    32221 aatggcgcag tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt
    32281 aaaagcctca cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt
    32341 tttaacggcg tgccagcaac agtcctagta ccagcccgac ggccgcgccc acaccaattc
    32401 cttgccaggg tttttcgtgg acgtagtcat cagcacgata aacagcctgc ttcgcccgat
    32461 agtaataact gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg
    32521 catgcgcttt cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct
    32581 cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat
    32641 tagacattct cgttctccat ttgcgtaaaa cctgtcacta actatagaca agagttgttg
    32701 attacgcctg aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga
    32761 cctccgtcac agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc
    32821 cgaggtaaac aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca
    32881 gctgctgccc cactttttca ccacgcaacg cttcactgac aatcacccga cctataacga
    32941 ccggctcaag atcatcatca cttttcagaa tcctcgcata cgccaccagt tcatcatttt
    33001 tccaaccgag aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag
    33061 ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg
    33121 aaaccgataa ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga
    33181 tgtatgcctt atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt
    33241 ttttaggtac ttcagccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta
    33301 acctgcaaca tccgacccag agcggacttt ggttgtttga ctgcggtgaa ggcacccagc
    33361 atcagctact gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc
    33421 ttcatggcga tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca
    33481 ttatccaacc cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc
    33541 tgcggattag cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg
    33601 aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat
    33661 gttatggcta tcgtattgaa gaacatgata aac
//
LOCUS       DAJVPT010000036        33119 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVPT010000036 DAJVPT010000000
VERSION     DAJVPT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..326
                     /locus_tag="OQM47_001668"
     CDS             <1..326
                     /locus_tag="OQM47_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="responsible for the influx of magnesium ions;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS1479859.1"
                     /translation="GGQLEQAREILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQN
                     RIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFK
                     RKNWL"
     gene            complement(367..747)
                     /locus_tag="OQM47_001669"
     CDS             complement(367..747)
                     /locus_tag="OQM47_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1479860.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(761..1177)
                     /locus_tag="OQM47_001670"
     CDS             complement(761..1177)
                     /locus_tag="OQM47_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479861.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(1236..2126)
                     /gene="rarD"
                     /locus_tag="OQM47_001671"
     CDS             complement(1236..2126)
                     /gene="rarD"
                     /locus_tag="OQM47_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1479862.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(2178..2645)
                     /locus_tag="OQM47_001672"
     CDS             complement(2178..2645)
                     /locus_tag="OQM47_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1479863.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2810..3679
                     /gene="pldA"
                     /locus_tag="OQM47_001673"
     CDS             2810..3679
                     /gene="pldA"
                     /locus_tag="OQM47_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1479864.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3812..5641
                     /gene="recQ"
                     /locus_tag="OQM47_001674"
     CDS             3812..5641
                     /gene="recQ"
                     /locus_tag="OQM47_001674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1479865.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5705..6325
                     /gene="rhtC"
                     /locus_tag="OQM47_001675"
     CDS             5705..6325
                     /gene="rhtC"
                     /locus_tag="OQM47_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1479866.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6387..7007)
                     /gene="rhtB"
                     /locus_tag="OQM47_001676"
     CDS             complement(6387..7007)
                     /gene="rhtB"
                     /locus_tag="OQM47_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1479867.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7118..8140
                     /gene="pldB"
                     /locus_tag="OQM47_001677"
     CDS             7118..8140
                     /gene="pldB"
                     /locus_tag="OQM47_001677"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1479868.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8148..8948
                     /gene="yigL"
                     /locus_tag="OQM47_001678"
     CDS             8148..8948
                     /gene="yigL"
                     /locus_tag="OQM47_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1479869.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9024..9923
                     /gene="bioP"
                     /locus_tag="OQM47_001679"
     CDS             9024..9923
                     /gene="bioP"
                     /locus_tag="OQM47_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1479870.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9811..10764)
                     /gene="metR"
                     /locus_tag="OQM47_001680"
     CDS             complement(9811..10764)
                     /gene="metR"
                     /locus_tag="OQM47_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1479871.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11000..13261
                     /gene="metE"
                     /locus_tag="OQM47_001681"
     CDS             11000..13261
                     /gene="metE"
                     /locus_tag="OQM47_001681"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1479872.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13300..14115)
                     /locus_tag="OQM47_001682"
     CDS             complement(13300..14115)
                     /locus_tag="OQM47_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1479873.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14377..15138
                     /gene="udp"
                     /locus_tag="OQM47_001683"
     CDS             14377..15138
                     /gene="udp"
                     /locus_tag="OQM47_001683"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1479874.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            15279..16706
                     /gene="rmuC"
                     /locus_tag="OQM47_001684"
     CDS             15279..16706
                     /gene="rmuC"
                     /locus_tag="OQM47_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1479875.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16801..17556
                     /gene="ubiE"
                     /locus_tag="OQM47_001685"
     CDS             16801..17556
                     /gene="ubiE"
                     /locus_tag="OQM47_001685"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1479876.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17570..18175
                     /gene="ubiJ"
                     /locus_tag="OQM47_001686"
     CDS             17570..18175
                     /gene="ubiJ"
                     /locus_tag="OQM47_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1479877.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18172..19812
                     /gene="ubiB"
                     /locus_tag="OQM47_001687"
     CDS             18172..19812
                     /gene="ubiB"
                     /locus_tag="OQM47_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1479878.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19891..20160
                     /gene="tatA"
                     /locus_tag="OQM47_001688"
     CDS             19891..20160
                     /gene="tatA"
                     /locus_tag="OQM47_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1479879.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20164..20679
                     /gene="tatB"
                     /locus_tag="OQM47_001689"
     CDS             20164..20679
                     /gene="tatB"
                     /locus_tag="OQM47_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1479880.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20682..21458
                     /gene="tatC"
                     /locus_tag="OQM47_001690"
     CDS             20682..21458
                     /gene="tatC"
                     /locus_tag="OQM47_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1479881.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21500..22282
                     /gene="tatD"
                     /locus_tag="OQM47_001691"
     CDS             21500..22282
                     /gene="tatD"
                     /locus_tag="OQM47_001691"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1479882.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22279..22767)
                     /gene="rfaH"
                     /locus_tag="OQM47_001692"
     CDS             complement(22279..22767)
                     /gene="rfaH"
                     /locus_tag="OQM47_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1479883.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22934..24427
                     /gene="ubiD"
                     /locus_tag="OQM47_001693"
     CDS             22934..24427
                     /gene="ubiD"
                     /locus_tag="OQM47_001693"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1479884.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24473..25174
                     /gene="fre"
                     /locus_tag="OQM47_001694"
     CDS             24473..25174
                     /gene="fre"
                     /locus_tag="OQM47_001694"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1479885.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25366..26529)
                     /gene="fadA"
                     /locus_tag="OQM47_001695"
     CDS             complement(25366..26529)
                     /gene="fadA"
                     /locus_tag="OQM47_001695"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1479886.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26539..28728)
                     /gene="fadB"
                     /locus_tag="OQM47_001696"
     CDS             complement(26539..28728)
                     /gene="fadB"
                     /locus_tag="OQM47_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1479887.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28918..30249
                     /gene="pepQ"
                     /locus_tag="OQM47_001697"
     CDS             28918..30249
                     /gene="pepQ"
                     /locus_tag="OQM47_001697"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1479888.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30249..30863
                     /locus_tag="OQM47_001698"
     CDS             30249..30863
                     /locus_tag="OQM47_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1479889.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30902..32353
                     /gene="trkH"
                     /locus_tag="OQM47_001699"
     CDS             30902..32353
                     /gene="trkH"
                     /locus_tag="OQM47_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1479890.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32365..32910
                     /gene="hemG"
                     /locus_tag="OQM47_001700"
     CDS             32365..32910
                     /gene="hemG"
                     /locus_tag="OQM47_001700"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1479891.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7799 a         8365 c         8964 g         7991 t
ORIGIN      
        1 cgggtgggca actggagcag gcgcgtgaaa tcctgcgcga tatcgaatcc ctgctgccgc
       61 ataacgaatc cctgttccag aaggtgaact tcctgatgca ggcggcaatg ggttttatca
      121 acatcgagca gaaccgcatc atcaaaattt tctcggtggt atccgtcgtg ttcctgccgc
      181 caacgctggt tgcttccagc tacgggatga actttgagtt tatgccagaa ctgaagtgga
      241 gtttcggcta ccctggcgcg attatcttta tgatcctcgc gggcctggca ccgtatctgt
      301 actttaagcg gaagaactgg ttgtaaaaag aaggcggtgg cttagcctgg ctaagccacc
      361 tgttattcaa aggctccagg tatttaaccc ttttacctct ttctcataaa accatttgtt
      421 cgtgttaaca gcaacatagg ctgctacggc aattcccaga atgttaacgc caattaatgc
      481 accaacaaat agagatagaa tgccaagtaa tagaactata attgcttttt tccataagcc
      541 cagaacaaat aaatagatcc aggaacagaa gaaagcaatg aaattcattt gaatagttaa
      601 tcgttgtctt acttttaatg ctttaaatgc tgctttatat tctggtgttg ctccccagaa
      661 tccaggaaag ccatgttgat cataaaaatt aaatcggtat ttccattttt cacttaatga
      721 atcatcgttc atatattcct tactcataaa tactccataa ctattgtttt gatgaatcag
      781 taggtgcaag cattagcata ctgaaagtgg aaaaataaca aatcaaaaaa atcatcgaac
      841 cattgcctga acaggcaaaa tcttcggcta tcattgtgat gatagagatg atatatactg
      901 ctaatgtacc aaaaacataa atttttatat atatgaaacc actatcacgg agtcgttggc
      961 aattcatgtt gatgacgaga taatggagtg cgatggtaga gactataaca agaaaacctg
     1021 cttccccatc gttaaaaaag ataataagaa aggcaaaaat gaaatttatt aaaataaata
     1081 aaaatatata tcggtgtctg gaaatcttac cgttagatgt tgggataaac atacgtaaca
     1141 taaattttac atccttgtgt gagtctcctg tcagcatggc aatatgccca ctctcataca
     1201 agagtcggca tattttttca gaatatattt attttttatt tggacgttct acgctgcgta
     1261 taaatcgcgt ccatcacaaa aattgccagc gccacccaaa taaaggtgaa agtcaccatc
     1321 ttatcggcac ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg
     1381 ccaatgtact gaaaaaagcc taacgttgag agacgcaggc gcgtggcagc ggcagtaaaa
     1441 cacaacagcg gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc
     1501 gggttttgcc ccatatggct ggttgaactg tcggcaatag caaacaggta aatcgccgcc
     1561 acgggcagca gccacatggt ttcaattaac atgccggttt gtgcttctac cgcaatcttc
     1621 ttgcgcacca ggccgtagaa ggcaaaacta aatgccagcc ccagcgcgat aataggtagc
     1681 gaaccaaaag tccacagctg gaccaacacg ccacatatcg ccagaatcac cgccagccat
     1741 tgcatccggc ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg
     1801 ttaataaagt aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac
     1861 agtagccagt tgccaccaat cagtacggca gagactgcca gcataaaaat tttctgtggc
     1921 gtctgaatca gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata
     1981 aagaaaaacg accagatcac gcgatgcgtc aggatctcat cggcgggcac gtagtaaatc
     2041 agcttgaagt atgctggcgc aataccccaa ataaaataag cggcaagagc gagtaatacg
     2101 ccctgccgcg tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt
     2161 tacctgcttt tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc
     2221 tgttcttcat tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg
     2281 ctactggtag cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca
     2341 atggtcccca tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg
     2401 gttaaggtac ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgact
     2461 ccgccgtgca aaatgctttg cgcccagtta cccaccatca tcggctgatt tttaaaggcc
     2521 agctgtgcga actccttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc
     2581 atgtgataaa caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca
     2641 gacatatcat ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt
     2701 tggtttctac tttaggaagg gataactaac ggcaatggag ttaagtatgt aaaatagccc
     2761 gcagaaaaac attcacctta tcaataattc gttacggaga acacgaccga tgcggactct
     2821 gcagggctgg ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac
     2881 ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca
     2941 ggagcatgac aatccgttca cgctctatcc ttatgacacc aactacctca tctacaccca
     3001 aaccagcgat ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa
     3061 ggatgaagta aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc
     3121 aaactcggtg ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga
     3181 agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac
     3241 cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc
     3301 taacgggcgt tcagacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc
     3361 agaaaacggt aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga
     3421 taacccggat atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg
     3481 tgatgcggtg ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga
     3541 gttaggctta agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg
     3601 ctatggcgaa tcgctcatcg actataactt caaccagacc cgtgtcggtg taggggttat
     3661 gctaaacgac ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca
     3721 ggattaccct ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg
     3781 cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga
     3841 gtccggagct aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca
     3901 ggaagaaatt atcgacactg tgctttccgg tcgcgattgc ctggttgtta tgcccactgg
     3961 tggcggaaaa tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt
     4021 tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat caactacagg ccaacggcgt
     4081 ggcggcggcg tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg
     4141 ctgccgcacc gggcaaattc gcttactgta tatcgccccg gaacgcctga tgctggataa
     4201 ctttcttgag catctggcgc actggaatcc ggtgttatta gccgtcgatg aagcgcactg
     4261 tatctcccaa tggggtcacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca
     4321 gcggttcccg acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca
     4381 ggatatcgtg cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg
     4441 tccgaatatt cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgcta
     4501 cgtgcaggaa cagcgcggta agtctggcat tatctactgc aacagccggg cgaaagtaga
     4561 agacaccgct gcgcgcctgc aaagcagggg tattagcgcg gcggcctatc atgccgggct
     4621 ggaaaataat gtccgcgccg acgtgcagga gaaattccag cgcgatgatc tgcaaattgt
     4681 ggtggcgacg gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca
     4741 ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga
     4801 tggcctgcct gcggaagcga tgctgtttta cgatccggct gatatggcgt ggctgcgccg
     4861 ttgtctggaa gagaagccgc aggggcagtt gcaggatatc gagcgccaca aactcaatgc
     4921 gatgggcgcg tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga actattttgg
     4981 cgaagggcgt caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta
     5041 cgacggttca accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt
     5101 tgggatgggt tatgtggtgg aagtgattcg tggtgctaat aaccagcgta tccgcgacta
     5161 tggtcatgac aaactgaaag tctatggtat gggccgtgat aaaagccatg aacattgggt
     5221 gagcgtgatc cgccagctga ttcatctcgg cctggtgacg caaaatattg cccagcattc
     5281 tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct ctttgcaact
     5341 tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa
     5401 ctatgatcgc aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag
     5461 taatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat
     5521 gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg
     5581 ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg acgaagagta
     5641 gtcagcagca taaaaaagtg ccagtatgaa gactccgtaa acgtttcccc cgcgagtcaa
     5701 atgtatgttg atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc
     5761 cggtcccgat ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat
     5821 gatgggcgtg ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgttgcttgg
     5881 cctgcatttg attatcgaaa aaatggcctg gctgcatacg ctgattatgg taggcggtgg
     5941 cctgtatctc tgctggatgg gttaccagat gctacgcggt gcactgaaaa aagaggctgt
     6001 atctgcgcct gcaccacagg ttgagctggc gaaaagcggg cgcagtttcc tgaaaggctt
     6061 actgaccaat ctcgccaatc cgaaagcgat tatctatttt ggttcggtat tctcattgtt
     6121 tgtcggcgat aacgttggca ctaccgagcg ctggggcatt ttcgcgttga ttattgtcga
     6181 aacactggcg tggtttaccg tcgttgccag tctgtttgcc ctgccgcaaa tgcgccgtgg
     6241 ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg
     6301 cattcatttg attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc
     6361 gtttacgccg catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc
     6421 accagcataa acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta
     6481 atccatagag caatcctttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc
     6541 acaatagtgg tgacgccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg
     6601 aattgcggaa atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa
     6661 actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aagatcaatt
     6721 gcgccagcgg cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac
     6781 tttaacactt caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc
     6841 agcacaatat gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga
     6901 taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt
     6961 aaaatgatcg atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg
     7021 tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgcttaaa
     7081 aaaacgattt tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg
     7141 ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg
     7201 tcagcgtgat gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt
     7261 tcgcgcacag caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt
     7321 aaaatatgcg gaactggcct acgacctgtt ccatttgggg tttgatgtct taatcatcga
     7381 ccatcgcggg cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa
     7441 tcgctttaat gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg
     7501 tccgtggcgt aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt
     7561 tctgcaacgc catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat
     7621 cgtgattcgt atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc
     7681 acgtttccgt gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat
     7741 caacgtactg acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga
     7801 cccaacgatt cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg
     7861 cgaacaggtg ttagccggtg cgggtgatga cgccacgcaa acgcttctct tacaagctga
     7921 agaggaacgc gtggtggata accgcatgca cgaccgtttt tgtgaactcc gcaccgccgc
     7981 gggccatcct gtcgaaggag gacggccgtt ggtaataaaa ggtgcttacc atgagatcct
     8041 ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag
     8101 gcataactca cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg
     8161 ttgcgtctga tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca
     8221 aagaaactct gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc
     8281 accacgttga tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct
     8341 ccaatggtgc gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc
     8401 gcgacattgc cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg
     8461 tttatcgcga cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta
     8521 aagaagcggt gttcaaatat gcgctgtatg agccgggatt actggagccg gaaggcatca
     8581 gcaaagtgtt tttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta
     8641 acgctcgctg gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga
     8701 tggcgggcgg cgtgtcgaaa ggccatgcgc tggaagcagt ggcgaagaaa ctgggctaca
     8761 gcctgaagga ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg ctgtcgatgg
     8821 cggggaaagg ctgcattatg ggcagtgcgc accagcgtct gaaagatctg catccggagc
     8881 tggaagttat tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt
     8941 tatcgtaatc gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact
     9001 cgttaatcaa agagttttcc attgtggcgt tacttatcat caccacgatt ctgtgggcct
     9061 tctcctttag cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc
     9121 tggtgcgcgt tggcttggcg gcactcgttt ttctgccgtt tctgcgtacc cgtagcaata
     9181 gcctgaaaac ggtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata
     9241 tgctgagttt ccgcgcttat ctctacctga cggtctccga attgttgctg ttcaccgtac
     9301 tgacgccgct ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg
     9361 gctatgcttt tagcgccttg ctggcggtga ttggtgccgg gattattcgc tatgatcagg
     9421 tcaccgacca tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca
     9481 tcggcatggt gggttacaaa cgcctgatgg aaactcgccc gatgccgcag tataacgcct
     9541 ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa
     9601 atgcgcagaa aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg
     9661 tggcttccgg gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa
     9721 cgctgggcat tatgaataat atgcacgttc cggcagggct gctggtaaac ctggctatct
     9781 ggcaccaaca gccgcactgg ccaacgttta ttacaggcgc gctggtgatc ctggcctcac
     9841 tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc
     9901 gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg
     9961 cacggcggcg tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc
    10021 ctggcgctca aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc
    10081 aaccatttga atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc
    10141 cggctgaaga aaatgccgcc agacatccag tcgactacgc tgcaccggat aaattaatag
    10201 cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt gccgccagtg gatggtcagg
    10261 tgctaacacc aggcgtactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg
    10321 caggatatcg gaggtcatca ccagatccag ctcgccctgt tgcaacgcgg gctgcgggtc
    10381 aaacgtcacg cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaatttcc
    10441 taaagcgggt gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt
    10501 ctgctgcggt tcattgcagg cttgcagtgc ctggctgatt tgcggcagca cctggtttgc
    10561 cagttgcaac aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa
    10621 tagccggaag ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt
    10681 ctgatgcaac gttgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt
    10741 tttcaggtgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt
    10801 gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaaatcatt
    10861 cgtcttttaa atttatggtg cgttggctgc gtttctccac cccggtcact tacttcagta
    10921 agctcccggc gatgaataaa cttgccgcct tccctaaatt caaaatccat aggatttaca
    10981 tataattaga ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc ctcgcgttgg
    11041 cctgcgtcgc gagctgaaaa aagcgcaaga aagttattgg gcggggaact ccacgcgtga
    11101 agaactgctg acagtagggc gtgaactgcg tgctcgtcac tgggatcaac aaaagcaagc
    11161 gggtatcgac ctgctgccgg tgggcgattt tgcctggtac gatcatgtac tgaccaccag
    11221 tttgctgctg ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg tagatatcga
    11281 caccctgttc cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga
    11341 aatgaccaaa tggtttaaca ccaactatca ctacatggtg ccggagttcg ttaaaggcca
    11401 acagttcaaa ctgacttgga cgcagctgct ggaggaagtg gacgaggcgc tggcgctgag
    11461 ccacaacgtt aagcctgtgc tgctggggcc ggttacttac ctgtggctgg ggaaagtgaa
    11521 aggtgaacag tttgaccgcc ttagcctgct gaacgacatt ctgccggttt atcagcaagt
    11581 gctggcagaa ctggcgaaac gcggcatcga gtgggtacag attgatgaac ccgcgctggt
    11641 actggaacta ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg
    11701 acaggtgaaa ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat
    11761 tactgcgctg cctgttcagg ggctgcatgt ggaccttgta catggtaaag ataacgttgt
    11821 tgaactgcac aagcgcctgc cttctgactg gctgctgtct gcgggtctga tcaatggtcg
    11881 taacgtctgg cgcgccgatc ttactgagaa atatgcgcaa attaaggaca ttgtcggcaa
    11941 acgtgatttg tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt
    12001 ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca
    12061 tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg
    12121 gagcgcccca attcaggcgc gtcgtcactc tactcgtgta cataatccgg cggtagaaaa
    12181 gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc
    12241 tgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga ttggttcctt
    12301 cccgcaaacc acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc
    12361 caacaactac cgcacgggca ttgcggaaca tatcaagcag gccattgttg agcaggaacg
    12421 tttgggactg gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt
    12481 tggcgagcac ctcgacggat ttgtctttac gcaaaacggt tgggtacaga gctacggttc
    12541 ccgctgcgtg aagccaccga ttgtcattgg tgacattagc cgcccggcac cgattaccgt
    12601 ggagtgggcg aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga tgctgacggg
    12661 gccggtgacc atactctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc
    12721 caaacagatt gcgctggcgc tgcgtgatga agtggccgat ctggaagccg ctggaattgg
    12781 catcatccag attgacgaac cggcgctgcg cgaaggttta ccgctgcgtc gtagcgactg
    12841 ggatgcgtat ctccagtggg gcgtagaggc cttccgtatc aacgccgccg tggcgaaaga
    12901 tgacacacaa atccacactc acatgtgtta ttgcgagttc aacgacatca tggattcgat
    12961 tgcggcgctg gacgcagacg tcatcaccat cgaaacctcg cgttccgaca tggagttgct
    13021 ggagtcgttt gaagagtttg attatccaaa tgaaatcggt cctggcgtct atgacattca
    13081 ctcgccaaac gtaccgagcg tggaatggat tgaagccttg ctgaagaaag cggcaaaacg
    13141 cattccggca gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc gcggctggcc
    13201 agaaacccgc gcggcactgg cgaacatggt gcaggcggcg cagaatttgc gtcgggggta
    13261 aatccaaacc gggtggtaat accacccggt cttttctcat tacagcgact tcttcccacc
    13321 atactgctta aaccattcca gcatacgctg ccagccatct ttcgcagatt cggcatgata
    13381 gctcgggcga taatcagcgt tgaatgcatg cccggcatcc gggtacacga taatctctgc
    13441 tttcgcatta gcagcccgca gcgcctggcg catcgtttca acgctctcct gcggaatgct
    13501 gttatcctga ccaccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac
    13561 aggttgtttc ggtgaattca gcgacttgtc gcccgtcagt ttgccgtacc acgccactgc
    13621 ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa
    13681 tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg
    13741 atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt
    13801 ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc
    13861 cagatacccc tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa
    13921 aatttcctgc actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg
    13981 gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc
    14041 gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg
    14101 ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat
    14161 agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga
    14221 atgaagagtg atttatgtga tatgaatcac ttttggtggg taaatttatg caacgcattt
    14281 gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc
    14341 ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat
    14401 ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg
    14461 gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc
    14521 gaattcacta cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt
    14581 atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc
    14641 ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg
    14701 gttaccacgg cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc
    14761 ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa atctattggc
    14821 gcgacaactc acgttggcgt gacagtttct tctgatacct tctacccagg tcaggaacgt
    14881 tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag
    14941 gcgatgggcg taatgaacta tgaaatggaa tctgcaactc tgctgaccat gtgtgcaagt
    15001 cagggcctgc gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc
    15061 ccgaatgctg agacgatgaa acaaaccgaa agccatgcgg tgaaaatcgt ggtggaagcg
    15121 gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt
    15181 atttttgcgt agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt
    15241 ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt
    15301 acgcagttat tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg
    15361 cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg
    15421 caaaacaaca aattacccaa agcgagcact ggcgtgcaga gtgtgaattg ctcaataacg
    15481 aagtgcgcag cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca
    15541 cgcggatgga agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg
    15601 agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc
    15661 gccgggttga tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac
    15721 aactggacgg tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc
    15781 ataccctgac ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag
    15841 cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg
    15901 tagtattgac gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc
    15961 aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc
    16021 cgcagggaaa agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt
    16081 ttaacgccga agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc
    16141 gtaaccatat ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc
    16201 tggattacgt gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgcc
    16261 agccggagct gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc
    16321 tgctggtggc gttgcgtact attgccaacc tgtggcgtta tgagcatcaa agccgtaacg
    16381 cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg
    16441 acatgtccgc gattggtcaa agtctcgaca aagcgcagga taactaccgc caggcaatga
    16501 aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag
    16561 gagtagaaat taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg
    16621 aagagtatcg acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg
    16681 atgaatataa tcagcagtcg cgctagccca ttaggggtag ttaagccggg tagaaatcta
    16741 gggcatcgac gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag
    16801 atggtggata agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa
    16861 aaagcggata tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat
    16921 gatttgatgt catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc
    16981 gtacgccgtg ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa
    17041 ttctcccgcc tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg
    17101 ctcaaaatgg gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt
    17161 caggcgaacg ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg
    17221 tttggtctgc gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg
    17281 aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc
    17341 aaagcctatg atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac
    17401 gacgccgaca gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc
    17461 ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggca
    17521 ggggttgtgg cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa
    17581 acctttagtg acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc
    17641 gctgaaaaca gcccgctcgc gtctgctggg taaagtattg cgcgtggagg taaaaggctt
    17701 ttcgacgtca ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc
    17761 aggcgatgct gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg
    17821 ccagcagctt accgcactga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt
    17881 ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg aactgctggc
    17941 cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa
    18001 gttcctgcat cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga
    18061 gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt
    18121 cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca
    18181 ggtgaagtac ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa
    18241 ctgatcccca aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg
    18301 atgccaaatc ggcataaaga caaactttta ggtgagcgac tacgactggc cctgcaagaa
    18361 ctggggccgg tttggatcaa gttcgggcaa atgttatcaa cccgccgcga tctttttcca
    18421 ccgcatattg ccgatcagct ggcgttattg caggacaaag ttgctccgtt tgatggcaag
    18481 ctggcgaagc agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac
    18541 gattttgaaa tcaagccgct ggcttctgct tctatcgccc aggttcatac cgcgcgattg
    18601 aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt gccggtcatt
    18661 aaagcggatc tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat
    18721 ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa
    18781 ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt tgaagacagc
    18841 ccgatgctct acatcccgga agtttaccct gactattgta gtgaagggat gatggtgatg
    18901 gagcgcattt acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa cggcactaac
    18961 atgaaattgc tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc
    19021 tttttccatg ccgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac
    19081 ccgaaatata tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc
    19141 tatctggcag aaaactttat cgccttcttt aatcgcgact atcgcaaagt ggcagagcta
    19201 cacgtcgatt ctggctgggt gccaccagat accaacgttg aagagttcga atttgccatt
    19261 cgtacggtct gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta
    19321 ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg
    19381 ttactccaga aaaccctgct ctatgtcgaa ggggtaggac gccagcttta tccgcagctc
    19441 gatttatgga aaacagcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt
    19501 cctgccctgg tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa
    19561 ctgcctgaac tggtttacga cagtttgcgc cagggcaagt atttacagca cagtgttgat
    19621 aagattgccc gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc
    19681 ggaattggcg ctacgttagt attaagtggc acattcttgt tggtcagccg acctgagtgg
    19741 gggctgatgc ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc
    19801 aaaacacgct gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt
    19861 ttaatcatca tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg
    19921 attattgccg tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc
    19981 gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag
    20041 gataaaacca gtcaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat
    20101 acgaatcagg aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa
    20161 tccgtgtttg atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt
    20221 ctggggccgc aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgcgcgttg
    20281 cgttcactgg cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt
    20341 caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa
    20401 gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga agcgttccta cgttgcaaac
    20461 gatcctgaaa aggcgagcga tgaagcgcac accatccata acccggtggt gaaagataat
    20521 gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa
    20581 cagaagccag aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa
    20641 accgctgcac cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc
    20701 aaccgcttat cacgcatctg attgagctgc gtaagcgtct gctgaactgc attatcgcgg
    20761 tgatcgtgat attcctgtgt ctggtctatt tcgccaatga catctatcac ttggtatccg
    20821 cgccattgat caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc
    20881 cgttctttac gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga
    20941 ttctctatca ggtgtgggca tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg
    21001 tggtgccgct gctggtttcc agctctctgc tgttttatat cggcatggca ttcgcctact
    21061 ttgtggtctt tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtgcagg
    21121 tatccaccga catcgccagc tatttaagct tcgttatggc gctgtttatg gcgtttggtg
    21181 tctcctttga agtgccggtg gcaattgtgc tgctgtgctg gatggggatt acctcgtcag
    21241 aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgttgc
    21301 tgacgccgcc ggatgtcttc tcgcaaacgc tattggcgat cccgatgtac tgtctgtttg
    21361 aaatcggtgt cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa
    21421 acgacgctga agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg
    21481 ttgtcatatg gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc
    21541 gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat
    21601 caccggcact aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc
    21661 ctgttggtca acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgcgactga
    21721 agaagcgatt attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct
    21781 cgactttaac cgtaactttt cgacgccgga agagcaggaa cgcgcttttg ttgctcagct
    21841 acgcattgcc gcagaattaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg
    21901 gtttatgaca ttgctggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt
    21961 taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata tcggcattac
    22021 cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc cgttgattcc
    22081 ggcggagaaa ttgctgatcg aaacagatgc gccgtatctg ctcccgcgcg atctcacgcc
    22141 aaagccatca tcccggcgca acgagccagc ccatctgccc catattttgc aacgtattgc
    22201 gcactggcgt ggagaagatg ccgcatggct ggctgccacc acggatgcca atgtcaaaac
    22261 actgtttggg attgcgtttt agagtttgcg gaactcggtg ttcttcacac tgtgcttaat
    22321 ctctttatta ataagattaa gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa
    22381 aatggcctga aagccttcga acgcgccttc ggtaataatc accttatcac ccggataagg
    22441 gggggccgga tcgacaatgt ctttcggttt atataccgat agctgatgaa ttaccgccga
    22501 tgggactatc gctggcgacg cgccaaagcg cacaaagtgg ctgacgccac gcgtagcgct
    22561 gatggtcgtg gtatgaatca cttctggatc aaattccaca aacaggtagt tagggaataa
    22621 cggttcactg actgcggtac gtttaccccg tacgattttt tccagggtga tcatcggagc
    22681 gaggcaatta accgcctgtc tctcaaggtg ttcctgggca cgttgaagtt gcccgcgctt
    22741 gcagtacagt aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg
    22801 atagcaaaag ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt
    22861 tcacgctaat ttaacaaatt tacagcattg caaagatgaa cgccgtataa tgggcgcaga
    22921 ttaagaggct acaatggacg ccatgaaata taacgattta cgcgacttcc tgacgctgct
    22981 tgaacagcag ggtgagctaa aacgtatcac gctcccggtg gatccgcatc tggaaatcac
    23041 tgaaattgct gaccgcactc tgcgtgctgg tgggcctgcg ctgttgttcg aaaaccctaa
    23101 aggctactca atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg tggcgatggg
    23161 catggggcag gaagatgttt cggcgctgcg tgaagttggt aaattattgg cgtttctgaa
    23221 agagccggag ccgccaaaag gtttccgcga cctgtttgat aaactgccgc agtttaagca
    23281 ggtgttaaac atgccgacaa agcgactgcg tggtgcgccc tgccaacaaa aaatcgtctc
    23341 tggcgatgac gtcgatctca atcgcattcc cattatgacc tgctggccgg aagatgccgc
    23401 gccgctgatt acctgggggc tgaccgtaac gcgcggcccg cataaagagc ggcagaatct
    23461 gggcatttat cgccagcagc tgattggtaa aaacaaactg attatgcgct ggctgtcgca
    23521 tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg catccgggcg aacgtttccc
    23581 ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc ggtgcagtca cccccgttcc
    23641 ggatacgctt tcagagtatg cgtttgccgg attgctacgc ggcaccaaaa ccgaagtagt
    23701 aaagtgtatt tccaatgacc tcgaagtgcc agccagtgcc gaaatcgtcc tggaagggta
    23761 tatcgatcct ggtgagatgg cgccggaagg gccgtatggt gaccacacag gttactataa
    23821 tgaagtcgat aatttcccgg tgtttaccgt gacgcatatt acccagcgtg aagatgcgat
    23881 ttaccattcc acctataccg ggcgtccgcc agatgagccc gcggtactgg gcgtggcgtt
    23941 gaacgaagtg tttgtaccga ttctgcaaaa acagttcccg gaaattgtcg atttttacct
    24001 gccgccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg
    24061 acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa
    24121 atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc
    24181 gattaccacc cgtatggacc cggcgcggga tactgttctg gtagaaaata cgcctattga
    24241 ttatctggat tttgcctcgc ctgtctctgg gctgggttca aaaatggggc tggatgccac
    24301 gaataaatgg ccgggggaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga
    24361 tgttgtcgcg catattgacg ccatctggga tgaactggct atttttaaca acggtaaaag
    24421 cgcctgatgc gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac
    24481 cttaagctgt aaagtgacct cggtagcagc tatcacggat accgtatatc gtgtccgcat
    24541 cgtgccagac gcggcctttt cttttcgtgc tggtcagtat ttgatggtag tgatggatga
    24601 gcgcgacaaa cgtccgttct caatggcctc gacgccggat gaaaaagggt ttatcgagct
    24661 gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa
    24721 agatcatcaa atcgtggtcg accttcccca cggagaagcg tggctgcgcg atgatgaaga
    24781 gcgtccgatg attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct
    24841 gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga
    24901 gcagcatctg tatgatctct gcgagcttga ggcgctttcg ttaaagcacc ttggtctgca
    24961 agtggtgccg gtggttgaac aaccggaagc aggctggcgt gggcgtactg gtaccgtgtt
    25021 aacggcggta ttgcaggatc atggtacgct ggcagagcat gatatctata ttgccggacg
    25081 ttttgagatg gcgaaaattg cccgtgacct gttttgcagt gagcgtaatg cgcgggaaga
    25141 tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg cccctgacag
    25201 gcgggaagaa cggcaactaa aactttattc agtggcattt agatctatgt cgcatccggc
    25261 aattggtgca cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg
    25321 gatcggtaga ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa
    25381 caccgtcgca ataccctgac ccagaccgat acacatcgtc gccagaccaa actgaacgtc
    25441 tttgcgttcc atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg
    25501 atgacccagc gcgatcgcac caccattgag gttgatcttc tcgtcaatct gctccattag
    25561 cccgagatct ttaatgcacg gcaggatctg cgcggcaaag gcttcgttca tctcaaacac
    25621 gccgatatcg ctggcagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg
    25681 gccgtaaccc ataatcgatg ggtcacaacc aacgaccgcc atcgaacgca cacgagcgcg
    25741 cggcttaaga cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc
    25801 atcggaaagt gcagaagatg tgcccgccgt taccgtaccg tttactggat caaacgccgg
    25861 acgcagcgtg gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa
    25921 ctgcttcagg acgccgtcgg catcgtgacc accagttggg atgatttcat ttttaaatgc
    25981 ggccgactgc gtggcggccc aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat
    26041 ttcacggctg ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc
    26101 cgctttggcg acattgcggc tcaggccggg gtgaaaatcg acgccgtgac tcatcggcac
    26161 atggcctata tgctccacgc cgccaaccag acatgcctgc gtatcgccag tcatgatcat
    26221 tcgtgctgcg tcatgcagtg cctgcatgga tgaaccacac aagcgattga cggtaaccgc
    26281 cgggacagag tgcggcactt ctgccagcag cgccgcgtta cgggcgatgt taaaaccctg
    26341 ctccagcgtc tgttgcacac aaccccagta aatatcgtcg agggccgccg cttccagcgc
    26401 cgggttacgc gccagcaggc tacgcattaa atgagcggag agatcttccg cacgcacgtt
    26461 acgaaaagca ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac
    26521 ctgttccatt gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac
    26581 cggaggatag tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcata
    26641 cagcgggccg aggtgctgat attgctgtgc catatcgagg tattttgcgc taccgagggt
    26701 gtccagccag cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc
    26761 catatccgct tccgccggag tggcgataat gccctcttcc agacagcgca ccacttcgtt
    26821 gaccatcggg atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggttg
    26881 gctcacttct gccagcaggt cttcaacggc ggcgtcttct tctttcttcg gcttaccttt
    26941 gctgtcttct ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc
    27001 aaacagcgcg tcgatggcat cgcggtaatc tttctgcatc cgctgcggga agcctgctgc
    27061 catgacagcc tgagcgtgat gcgcggtatc aatgcccaca acgtccagca gatatgccgg
    27121 gcccatcggc cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc
    27181 gtcgcgcagc agctggctga aaccggcgaa atacgggaac agcacgcggt taacaaagaa
    27241 gccggggcag tcgttaacca caatcggcgt cttgcccatc ttgctcgccc aggcgacaac
    27301 tttcgcgatg gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcattcg
    27361 gtggaccggg ttaaagaagt gcatcccgca gaagttttcc gggcgttcca gcgcgttggc
    27421 cagttcgctg ataggaatgg ttgaagtgtt agacgccagc acggtatcct ggcgtacttt
    27481 ttgttcggtt tctgccagta cggctttttt cactttcggg ttttcaacaa ccgcttctac
    27541 cacaatatcc acgcggtcaa atccggcgta gtcgagcgtt gggtggatcg tggagatcac
    27601 gccagccagt ttcagaccat cgattttgcc gcgctcaagc tgcttgttca gcagtttcgc
    27661 ggcttcggtc atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac
    27721 gcctttccac gcggactggt aagcgatacc gccacccatg atgcctgcgc ccagcacagc
    27781 agcctgtttc ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg
    27841 atcgttaagg aaaatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa
    27901 acttttgttt tccagattta aggcttcttc acgaccaaaa cgggccgcag cttcaatggt
    27961 ttttactgcg gtgatggggg ccggataatg tttccccgcc gtttgcgcga ccattccttt
    28021 agcgatggtg aagctcatgg tggcttcaat cttgctcagt tttagcggtt cctgcttcgg
    28081 ctgacgtttc gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc
    28141 cccttcaatc agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg
    28201 atccgcgccg acatctttac cggcggcaat gatttccagt gcactgtcag cgcccagcat
    28261 acgtggcata cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag
    28321 accgatgcgc agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc
    28381 accacccagc gcatagccgt tgacggcagc aatggtcggc accggcagat cttccaggcg
    28441 attaaacacg ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa
    28501 cagggacaaa aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag
    28561 cagcagccct tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc
    28621 gacggtcgca gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat
    28681 gccatcttcc agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg
    28741 aatccgcaag gtaatctggt cgtaccagat gcatcggagt gtgtgttttg tgttaaaaat
    28801 atgcaaataa aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg
    28861 gatgaacgat gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg
    28921 gaatcactgg cctcgctcta taaaaatcat atagctacct tacaagaacg gactcgcgat
    28981 gcgctggcgc gcttcaagct ggatgcatta cttattcact ccggcgagct gttcaacgtt
    29041 tttctcgacg atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg
    29101 gtaactcagg tgccaaactg ctggttgctg gtggatggcg tgaacaagcc gaaactgtgg
    29161 ttctatctgc cggttgatta ctggcacaac gtcgaaccgc tgccgacctc cttctggact
    29221 gaagatgtgg aagtgatcgc gctgccgaaa gccgatggca ttggtagcct gctgcctgct
    29281 gcgcgcggca atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt
    29341 gaggccagca acatcaaccc gaaaggggtg atcgactacc tgcattatta ccgctccttc
    29401 aaaaccgagt acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caatggtcat
    29461 cgtgcggcag aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa tattgcctat
    29521 ctgaccgcga ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcgcttaac
    29581 gaacacgctg cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc
    29641 agcttcctgc tggatgccgg ggccgaatat aacggctatg cggctgacct gacccgtacc
    29701 tggtcggcaa aaagcgacaa cgactacgca cagctggtga aagacgtaaa tgatgaacaa
    29761 ctggcgctga tcgccaccat gaaagcaggc gtcagctatg tggattacca catccagttc
    29821 catcagcgca tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa
    29881 gcgatggtcg aaaacgatct caccggaccg tttatgccgc acggtattgg ccatccgctg
    29941 ggcctgcagg tgcatgacgt cgccggtttt atgcaggatg atagcggtac acacctcgcg
    30001 gcaccggcaa aatatccgta cctgcgctac acccgtattc tccagccggg catggtgtta
    30061 accatcgaac cgggtatcta cttcattgaa tcgcttctgg caccgtggcg tgaagggcag
    30121 ttcagcaagc acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt
    30181 atcgaagaca acgtggtgat ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa
    30241 ctggcgtgat ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca
    30301 aaaagagccg cttcataacg atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt
    30361 ttgttgaatc ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg
    30421 cgggtgcgcc ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa
    30481 cggcaggtaa accgatgctc gcccagctaa tgggcagcgg cgtcggggaa attaccgctg
    30541 tggtagtgcg ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg
    30601 gcggcggcgt gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg
    30661 aatatacttt gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt
    30721 gtgacggcaa aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc
    30781 cggcggcgaa agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc
    30841 aattgttagc gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga
    30901 gatgcatttt cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac
    30961 catgattatc cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac
    31021 ccagaccttt tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga
    31081 gaagggcgaa ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt ggaccgtgct
    31141 gggtagtgtc ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga
    31201 tgcgtttttt gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct
    31261 ggattcgctc cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat
    31321 ggggatcatc gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct
    31381 ctatcgcgca gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga
    31441 aacggcgaaa actctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt
    31501 gtggtttgcc ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat
    31561 tggcggcttc tcgacacatg atgccagtat cggttatttc gacagtccga ctattaacac
    31621 tatcattgct atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt
    31681 aagtgggcgt agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt
    31741 acagtttacg ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc
    31801 ggcgctgatg acaattaacc aggcgttttt ccaggtggta tcgatggcga caaccgccgg
    31861 gtttacgact gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc
    31921 agcttttatc ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct
    31981 gctgctgttt aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta
    32041 tagcattaag ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt
    32101 tttctccgcc tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt
    32161 ggatgatttt tctgcctttg cctcagttgt tgcgacattg aataacctgg ggccagggct
    32221 tggcgtggtt gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc
    32281 caacatgctg tttggtcgtc tcgaggtctt tacattactg gtgctcttta ccccgacttt
    32341 ctggcgtgaa tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga
    32401 caaacgcgcg agattgcctc ctacctggct tcggaactga aagaactggg gatccagacg
    32461 gatgtcgcca atgtgcaccg cattgaagaa ccacaatggg aaaactatga ccgtgtggtc
    32521 attggtgctt ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa
    32581 catgcgacgc gactgaattc gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc
    32641 aaaccggaga agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg
    32701 caatggcgtc ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc
    32761 tggtacgacc gttttatgct caagctgatt atgaagatgt caggcggtga aacggatacg
    32821 cgcaaagaag ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc
    32881 catttaaccg acaaaccgac gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa
    32941 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    33001 cacggaacaa cggcaaacac gcagccgggt cagcggggtt ctcctgagaa ttccgacaga
    33061 gaaagcaaaa ataaatgctt gactttgtag cgggaaagcg tattatgaat accccgcgc
//
LOCUS       DAJVPT010000037        32469 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVPT010000037 DAJVPT010000000
VERSION     DAJVPT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            77..1354
                     /gene="waaA"
                     /locus_tag="OQM47_001701"
     CDS             77..1354
                     /gene="waaA"
                     /locus_tag="OQM47_001701"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1479892.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1362..1841
                     /gene="coaD"
                     /locus_tag="OQM47_001702"
     CDS             1362..1841
                     /gene="coaD"
                     /locus_tag="OQM47_001702"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1479893.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1880..2689)
                     /gene="mutM"
                     /locus_tag="OQM47_001703"
     CDS             complement(1880..2689)
                     /gene="mutM"
                     /locus_tag="OQM47_001703"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1479894.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2787..2954)
                     /gene="rpmG"
                     /locus_tag="OQM47_001704"
     CDS             complement(2787..2954)
                     /gene="rpmG"
                     /locus_tag="OQM47_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1479895.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2975..3211)
                     /gene="rpmB"
                     /locus_tag="OQM47_001705"
     CDS             complement(2975..3211)
                     /gene="rpmB"
                     /locus_tag="OQM47_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1479896.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3428..4096)
                     /gene="radC"
                     /locus_tag="OQM47_001706"
     CDS             complement(3428..4096)
                     /gene="radC"
                     /locus_tag="OQM47_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1479897.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4268..5488
                     /gene="coaBC"
                     /locus_tag="OQM47_001707"
     CDS             4268..5488
                     /gene="coaBC"
                     /locus_tag="OQM47_001707"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1479898.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5466..5924
                     /gene="dut"
                     /locus_tag="OQM47_001708"
     CDS             5466..5924
                     /gene="dut"
                     /locus_tag="OQM47_001708"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1479899.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6031..6627
                     /gene="slmA"
                     /locus_tag="OQM47_001709"
     CDS             6031..6627
                     /gene="slmA"
                     /locus_tag="OQM47_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1479900.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6664..7305)
                     /gene="pyrE"
                     /locus_tag="OQM47_001710"
     CDS             complement(6664..7305)
                     /gene="pyrE"
                     /locus_tag="OQM47_001710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1479901.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7371..8087)
                     /gene="rph"
                     /locus_tag="OQM47_001711"
     CDS             complement(7371..8087)
                     /gene="rph"
                     /locus_tag="OQM47_001711"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1479902.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8214..9077
                     /locus_tag="OQM47_001712"
     CDS             8214..9077
                     /locus_tag="OQM47_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1479903.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9298..10122
                     /gene="dinD"
                     /locus_tag="OQM47_001713"
     CDS             9298..10122
                     /gene="dinD"
                     /locus_tag="OQM47_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1479904.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10413..11030
                     /locus_tag="OQM47_001714"
     CDS             10413..11030
                     /locus_tag="OQM47_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1479905.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11027..12709)
                     /gene="ligB"
                     /locus_tag="OQM47_001715"
     CDS             complement(11027..12709)
                     /gene="ligB"
                     /locus_tag="OQM47_001715"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1479906.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12967..13590
                     /gene="gmk"
                     /locus_tag="OQM47_001716"
     CDS             12967..13590
                     /gene="gmk"
                     /locus_tag="OQM47_001716"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1479907.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13645..13920
                     /gene="rpoZ"
                     /locus_tag="OQM47_001717"
     CDS             13645..13920
                     /gene="rpoZ"
                     /locus_tag="OQM47_001717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1479908.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13939..16047
                     /gene="spoT"
                     /locus_tag="OQM47_001718"
     CDS             13939..16047
                     /gene="spoT"
                     /locus_tag="OQM47_001718"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1479909.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16054..16743
                     /gene="trmH"
                     /locus_tag="OQM47_001719"
     CDS             16054..16743
                     /gene="trmH"
                     /locus_tag="OQM47_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1479910.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16749..18830
                     /gene="recG"
                     /locus_tag="OQM47_001720"
     CDS             16749..18830
                     /gene="recG"
                     /locus_tag="OQM47_001720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1479911.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18864..20069)
                     /gene="gltS"
                     /locus_tag="OQM47_001721"
     CDS             complement(18864..20069)
                     /gene="gltS"
                     /locus_tag="OQM47_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1479912.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20349..21740
                     /gene="xanP"
                     /locus_tag="OQM47_001722"
     CDS             20349..21740
                     /gene="xanP"
                     /locus_tag="OQM47_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1479913.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21861..23570
                     /locus_tag="OQM47_001723"
     CDS             21861..23570
                     /locus_tag="OQM47_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1479914.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23623..25941)
                     /gene="yicI"
                     /locus_tag="OQM47_001724"
     CDS             complement(23623..25941)
                     /gene="yicI"
                     /locus_tag="OQM47_001724"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1479915.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25951..27334)
                     /locus_tag="OQM47_001725"
                     /pseudo
     CDS             complement(25951..27334)
                     /locus_tag="OQM47_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27627..27721
                     /locus_tag="OQM47_001726"
     tRNA            27627..27721
                     /locus_tag="OQM47_001726"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27661..27663,aa:Sec,seq:tca)
     gene            27993..29204
                     /locus_tag="OQM47_001727"
     CDS             27993..29204
                     /locus_tag="OQM47_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1479916.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29616..30659
                     /locus_tag="OQM47_001728"
     CDS             29616..30659
                     /locus_tag="OQM47_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1479917.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OQM47_001729"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OQM47_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32469)
                     /locus_tag="OQM47_001730"
                     /pseudo
     CDS             complement(31632..32469)
                     /locus_tag="OQM47_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
BASE COUNT         8119 a         8590 c         8055 g         7705 t
ORIGIN      
        1 aaaacgggaa aagtaatggt aaagccacag ctaaatacat agaatcccca gcacatccat
       61 aagtcagcta tttactatgc tcgaattgct ttacaccgcc cttctctacc ttattcagcc
      121 gctgatctgg atacggctct gggtgcgcgg acgtaaggct ccggcctatc gaaaacgctg
      181 gggtgaacgt tacggttttt accgccatcc gctaaaacca ggcggcatta tgctgcactc
      241 cgtctccgtc ggtgaaactc tggcggcgat cccgttggtg cgcgcgctgc gtcatcgtta
      301 tcctgattta ccgattaccg ttacaaccat gacgccaacc ggttcggagc gcgtacaatc
      361 ggctttcggg aaggatgttc agcacgttta tctgccgtat gacctgcccg atgcgctcaa
      421 ccgtttcctg aataaagtcg accccaaact ggtgttgatt atggaaaccg aactatggcc
      481 taacctgatt gcggcgctac ataaacgtaa aattccgctg gtgatcgcta acgcgcgact
      541 ctctgcccgc tcggccgcag gttatgccaa actgggtaaa ttcgtccgcc gcttgctgcg
      601 tcgtattacg ctgattgctg cgcaaaatga agaagatggt gcacgttttg tggcgctggg
      661 cgcaaaaaat aaccaggtaa ccgttaccgg tagcctgaaa ttcgatattt ctgtaactcc
      721 gcagttggct gctaaagccg tgacgctacg ccgccaatgg gcaccacacc gcccggtatg
      781 gattgccacc agcactcacg aaggtgaaga gagcgtggtc attgccgcac atcaggcatt
      841 gttacagcaa ttcccgaatt tattgctcat cctggtaccc cgtcatccag aacgcttccc
      901 ggatgcgatt aaccttgtcc gccaggctgg actaagctat atcacacgct cttcagggga
      961 agtcccctcc accagcacgc aggttgtggt tggcgatacg atgggcgagt tgatgttact
     1021 gtacggcatt gccgatctcg cctttgttgg cggttcactg gttgaacgtg gtgggcataa
     1081 tccgctggaa gctgccgcac acgctattcc ggtattgatg gggccgcata cttttaactt
     1141 taaagacatt tgcgcgcggc tggagcaggc aagcgggctt attaccgtta ccgatgccac
     1201 tacgcttgca aaagaggttt cctctttact caccgacgcc gtttaccgta gtttctatgg
     1261 ccgtcatgcc gttgaagtac tgtatcaaaa ccagggcgcg ctacagcgtc tgcttcaact
     1321 gctggaacct tacctgccac cgaaaacgca ttgaggttgt tatgcaaaaa cgggcgattt
     1381 atccgggtac tttcgatccc attaccaatg gtcatatcga tatcgtgacg cgcgccacgc
     1441 agatgttcga tcacgttatt ctggcgattg ccgccagccc cagtaaaaaa ccgatgttta
     1501 ccctggaaga gcgtgtggca ctggcacagc aggcaaccgc gcatctgggg aacgtggaag
     1561 tggtcgggtt tagtgatttg atggcgaact tcgcccgtaa tcaacacgct acggtgctga
     1621 ttcgtggcct gcgtgcggtg gcggattttg aatatgaaat gcagctggcg catatgaatc
     1681 gccacttaat gccggaactg gaaagtgtgt ttctgatgcc gtcgaaagag tggtcgttta
     1741 tctcttcatc gttggtgaaa gaggtggcgc gccatcaggg cgatgtcacc catttcctgc
     1801 cggagaatgt ccatcaggcg ctgatggcaa aattagcgta gcgtttatgc cggatggtat
     1861 gccatccggc tcgcatgaat tacttctggc actgccgaca ataaaacgtt gcccgctgcg
     1921 catgtttagt cgccacaatc ggcgtaccgc acacccgaca cggctcacct tttcgcccgt
     1981 aaacctgcaa ttcctgcgcg aaatagcccg gtttaccatc actttgcaga aaatctttca
     2041 gcgttgtacc accctgctca atcgaacgca gcaacaccgc tttaatcacc cgagctaaca
     2101 attcacactc tgccagcgac agtgatgacg ccagccgatc cggatggatc cccgccgcaa
     2161 acagtgattc gctggcatag atattcccta cccctaccac cagcttgtta tccatcagcc
     2221 acggtttaat cgccgttttt ttcttcgcgc acttctgatg cagatactca ccattgaaat
     2281 cgtcgctaag cggctccggt ccaagatggg ccagcacatt atgcccttcc agctctttgg
     2341 tccacagcca ggcaccaaag cggcgcggat cggtgtagcg cagcacttta ccgttgctca
     2401 tcaccaaatc cacatggtca tgcttttcag ggggaagttc ttctggaagg atgcgcaggc
     2461 tgccagacat ccccaaatga atgataatcc agccctcagg cagctccagc agcagatatt
     2521 tagcccgccg ctgcacgcta agcactggtt gatcgcttaa tcggtagatc tcttctgaaa
     2581 ccggccagcg caagcgtccg ttgcgcacca ccgcatgaag aatggttgca ccaacgagat
     2641 gcggttctat gccgcgacgg ctggtttcaa cttcgggtaa ttcaggcata gcatctccag
     2701 gaatgaacag atgcaatcaa tatgggggca agcagataac aaaaaacccc gccggagcga
     2761 ggtttttcgt tacatcaaag cgagaattat ttgattttcg cttctttgta gatcacgtgc
     2821 tgacgaacaa ctggatcgaa ttttttcagt tccagttttt ccggcttagt acgtttgttc
     2881 ttcgtagtgg tatagaagtg accagtacca gcagaagaaa ccagcttgat tttctcacga
     2941 atacctttag ccatgattta tttcctctaa gtacttagta cttttcgcca cgggcacgca
     3001 gttcagccag aactgtatcg atgccttttt tatcgattac acgcatacct ttagcagata
     3061 cgcgcagggt gacaaaacgc ttctcgctct caacccagaa acggtgagag tgcaggttcg
     3121 gcaggaaacg gcgtttagtc gcgttcagtg cgtgggaacg gttgttaccg gtcaccggac
     3181 gcttgccagt aacttggcag actcgggaca tgtctattct ccaaaaatca aattagctcg
     3241 agcttcgtat ggggtattgg cgcctcgtca ggctttacag cccggtcatc gcagttctat
     3301 gtgaactctc gattgccagg cccaaatgcc aaacccgaga ttctcaaagg tggcgtagta
     3361 tacgctgact cagcgatgtg ctcaagtccc gaacagacaa agatcccgaa ggatcgcgca
     3421 tagcgggtta aatccagccg cgttcggcaa aagaaacata ctctccacgc ccaatcacga
     3481 tatggtcgag cacgcgtaaa tccatgaact gacaactctt tattatccgt tcagtaatga
     3541 gtttatccgc tttactgggt tcagcacaac ccgaagggtg attatgtgca aggatcagcg
     3601 ccgaggcgtt tatttttatc gcttcgcgga taatttcccg aggatggact tcaacatggt
     3661 ttagcgtgcc ggaaaaaaga cggctatgcg ttataacccg gtgttgtgag tcgagaaaga
     3721 tcaccataaa gatctcccgc tcctcacccg tgagctggct ttgtaaaaat tcccgcgtca
     3781 tctccgggct gagtaaaggg ctttcttcac gcatccgcac gttgtagtaa cgtcgcgcca
     3841 gttcagcaat cccctttaac tgggcaaatt tcgctacgcc aattccatga acaccactaa
     3901 attgttcata ttcagaggtt aacaagccat aaagagagcc gaaattctcc agcatctctt
     3961 ttgccagggt taatacatct ttaccgcgcg ttccggtacg cagaaatagc gccagcagct
     4021 cgacatccgt taaggcgcta ataccaaact tcagcatttt ttcgcgcggc atcaacagct
     4081 gtgcattgtt tttcaccttc acctcctttg tggtgctcgc atcctgtcat agccgttccg
     4141 ttaaatcgac ggccagtttt caatcctgga aagagcctcg caaagtgaat ctcaggtgaa
     4201 ggcacgacaa caaaagtatt gtgataaaat cgccaacttc tggtgtcacc caacaggaaa
     4261 aatcatcatg agcctggccg gtaaaaaaat cgttctcggc gttagcggcg gtattgctgc
     4321 ctataaaacc cctgaactgg tgcgtcgttt gcgcgatcgc ggggccgacg tccgcgtagc
     4381 catgaccgaa gcggcaaaag cctttatcac cccacttagc ttgcaggcgg tttctggtta
     4441 tcccgtttcc gacagtctgc ttgacccggc agccgaagcc gctatgggcc atattgagct
     4501 gggtaaatgg gctgatttag tgattctcgc ccctgccacg gcagatttga ttgcccgtgt
     4561 tgctgccgga atggcgaatg acctggtatc gacgatttgt ctggctacac ctgcgcctgt
     4621 agccgtgctc cccgccatga accagcagat gtaccgtgcc gctgccacgc agcataattt
     4681 agaggtgctt gcttcccgtg gtttgctcat ctgggggcca gacagtggca gtcaggcttg
     4741 tggtgatatc ggtcctggac gaatgctcga tccgttaacc attgtggata tggcggtggc
     4801 gcatttttcg cccgtcaacg acctgaaaca tctgaacatt atgattaccg ccggtccgac
     4861 gcgtgaaccg ctcgatccgg tgcgttatat ctctaatcac agctccggca agatgggttt
     4921 tgctatcgcc gccgctgctg cccgtcgtgg cgcgaacgtc acgctggtat caggtccagt
     4981 ttcactaccg acgccaccgt ttgttaaacg tgttgatgtg atgaccgcgc tggaaatgga
     5041 agccgccgtg aatgcttctg tacagcagca aaatattttt atcggttgcg ccgccgtggc
     5101 ggattatcgc gcagctaccg tggccccaga aaaaatcaaa aagcaggcca cgcagggtga
     5161 tgaattaaca ataaaaatgg ttaaaaaccc cgatatcgtc gcaggcgttg ccgcactaaa
     5221 agaccatcga ccctacgtcg ttggatttgc cgccgaaaca aataatgtgg aagaatacgc
     5281 ccggcaaaaa cgtatccgta aaaaccttga tctgatctgc gcgaacgatg tttcccagcc
     5341 aactcaagga tttaacagcg acaacaacgc attacacctt ttctggcagg acggagataa
     5401 agtcttaccg cttgagcgca aagagctcct tggccaatta ttactcgacg agatcgtgac
     5461 ccgttatgat gaaaaaaatc gacgttaaga ttctggaccc gcgcgttagg aaggaatttc
     5521 cgctcccgac ttatgccacc tctggctctg ccggacttga cctgcgtgcc tgtctcgacg
     5581 acgccgtaga actggctccg ggtgacacta cgctggttcc gaccgggctg gcgattcata
     5641 ttgccgatcc ttcactggcg gcaatgatgc tgccgcgctc cggattggga cataagcacg
     5701 gtatcgtgct tggtaacctg gtaggattga tcgattccga ctatcagggc cagttgatga
     5761 tttccgtgtg gaaccgtggt caggacagct tcaccattca acctggcgaa cgcatcgccc
     5821 agatgatttt tgttccggta gtacaggctg aatttaatct ggtggaagat ttcgacgcca
     5881 ccgaccgcgg tgaaggcggc tttggtcact ctggtcgtca gtaacacata cgcatccgaa
     5941 taacgtcata acatagccgc aaacatttcg tttgcggtca tagcgtgggt gccgcctggc
     6001 aagtgcttat tttcaggggt attttgtaac atggcagaaa aacaaactgc gaaaaggaac
     6061 cgtcgcgagg aaatacttca gtctctggcg ctgatgctgg aatccagcga tggaagccaa
     6121 cgtatcacga cggcaaaact ggccgcctct gtcggcgttt ccgaagcggc actgtatcgc
     6181 cacttcccca gtaagacccg catgttcgat agcctgattg agtttatcga agatagcctg
     6241 attactcgca tcaacctgat tctgaaagat gagaaagaca ccacagcgcg cctgcgtctg
     6301 attgtgttgc tgcttctcgg ttttggtgag cgtaatcctg gcctgacccg catcctcact
     6361 ggtcatgcgc taatgtttga acaggatcgc ctgcaagggc gcatcaacca gctgttcgag
     6421 cgtattgaag cgcagctgcg ccaggtattg cgtgaaaaga gaatgcgtga gggtgaaggt
     6481 tacaccaccg atgaaaccct gctggcaagc cagatcctgg ccttctgtga aggtatgctg
     6541 tcacgttttg tccgcagcga atttaaatac cgcccgacgg atgattttga cgcccgctgg
     6601 ccgctaattg ccgcgcagtt gcagtaatat gacgccggat gacttttcat ccggcgagtt
     6661 tctttaaacg ccaaactctt cgcgataggc cttaaccgcc gccagatgtt ccgccatttc
     6721 cggcttctct tccagatagg caatcagatc tttcagggtg atgatagaga tcactttgca
     6781 gttgtaatca cgctcaactt cctgaatcgc cgaaatctcg ccgcgcccgc gttcctgacg
     6841 atcgagcgaa atcaacacgc cagcaagcgt cgcgccattg gcctgaataa tctccatcga
     6901 ctcgcgaatc gccgttccgg cggtgatcac atcatctacc agcattacgc gtccttgtaa
     6961 cgcgctacca accagattgc cgccttcacc gtggtctttt gcttctttgc ggttaaagca
     7021 gtacggcagg tccaggtcgt gatgctccgc cagtgccaca gcggttgtgg tggcaatcgg
     7081 gatccctttg taagcagggc caaacagcag atcgaactca atgccggaat ccaccaacgc
     7141 ttcagcgtaa aaacggccta acagtgccag atcgcgcccg gtattaaaca gcccggcgtt
     7201 gaagaaatag gggcttttgc gcccggattt cagcgtaaac tcgccaaact ttaacacctg
     7261 cttgctaagc gcaaattcaa taaactggcg ctgatatgat ttcatgcctt cgctcctcat
     7321 cttacttttc tacagacaaa aaaaaggcga ctcatcagtc gccttaaaaa tcagtttgcc
     7381 agcgccgcct tctgcgtcgc tacaatggat tcgattcccc ctcgggccag cgccaacaag
     7441 gtgagtagct cttcatgggt gaacggctcg ccttctgccg tcccctgcac ttcaatgatg
     7501 cgcccgtctt cggtcatcac tacgttcatg tcggtctctg cggctgagtc ttcaacgtat
     7561 tccagatcgc aaattgcttc accgtttaca ataccgacag aaactgcggc taccatccct
     7621 ttcatcgggt tggttttcag cttgccgttt tccaccagct tctgtagcgc atctgccagc
     7681 gccacgcagg cacccgtaat cgacgcggta cgagtgccac catcagcctg aagcacgtcg
     7741 cagtccagcg taatggtgaa ctcacccagc gctttcaaat ctactgccgc gcgaagagca
     7801 cgggcgatca gacgctggat ttccattgtg cgtccaccct gcttaccttt cgccgcttca
     7861 cgagcgttac gggtgtgggt agaacgtggc agcatgccgt actctgcggt gatccagccc
     7921 tggccctgac ctttcaggaa gcgcggcacg ccttcttcaa tagaggcggt acacaatact
     7981 ttggtatcgc caaattcgac cagcaccgag ccttctgcat gttttgtata gttacgagtc
     8041 agggtaacgg gacgcacctg attattgcta cggcctgctg gacgcatatt gaaatctccg
     8101 gcttgaaaca aatgtggctg cgcattatac ggacttccgg cggttattcc tatcctgaca
     8161 aggcatcgat ggctataatc cttccacctc tccttttata aacaggaacg tctatgatcc
     8221 gcagtatgac cgcctacgcc cggcgtgaaa tcaagggtga atgggggagc gcaacctggg
     8281 aaatgcgctc ggtaaaccag cgttatctgg aaacttactt tcgtctgccg gagcagttcc
     8341 gtagccttga acctgtcgtt cgcgagcgta ttcgttctcg cctgacgcgc ggtaaagtgg
     8401 aatgtaccct gcgctatgag ccagatgtta gcgcacaagg tgagctgatc ctcaatgaaa
     8461 aactggctaa acagctggta actgccgcga actgggtaaa aatgaagagt gacgaagggg
     8521 aaatcaaccc ggttgatatt ctacgctggc cgggcgtgat ggcagcccag gagcaggatc
     8581 tcgacgcgat tgccgctgaa attctcgcgg cgttggatgg tacgctggac gactttatcg
     8641 tcgcgcgcga aaccgaaggt caggcactga aagcattgat cgagcagcgt ctggaaggcg
     8701 tcaccgccga agtggtcaaa gtccgcgccc atatgccgga aattctgcaa tggcagcgtg
     8761 agcgtctggt cgcgaagctg gaagatgctc aggttcagtt ggaaaacaac cgtctggagc
     8821 aggagttggt tctgctggca caacgaattg acgttgccga agagctggac cgcctcgaag
     8881 cgcatgttaa agagacgtac aacattctga agaaaaaaga agcggttggt cgtcgtctgg
     8941 actttatgat gcaggagttc aaccgtgagt cgaacactct tgcgtcgaaa tctatcaatg
     9001 ccgaagtgac aaactccgcc atcgagctga aagtgttgat tgagcagatg cgcgagcaga
     9061 ttcagaatat tgagtaactc gtaacaccaa ttctgacttt tcagccttca caaatgccgc
     9121 ccactcaaac agagcggcat ttttcttccc cgcaacttca attctgtttt gcgtgcctgc
     9181 tccagatttt gcgatgtttt tttgcccagc acactgagaa cgtgagatac tcacaactgt
     9241 atataaatac agttacagat ttactttctt tgcaatcgat atcacatgga gtgggcaatg
     9301 aacgaacatc atcaaccttt tgaagagata aaactgatta atgcaaacgg agcagaacaa
     9361 tggtcagcaa gacaacttgg gaaactactg ggttattcag agtatcgtca ctttatacct
     9421 gtattaacgc gcgccaaaga ggcctgtgag aacagtggtc acacaattga tgaccatttc
     9481 gaggagatcc tcgatatggt caaaattggc tcaaatgcca aaagagcatt aaaagacatc
     9541 gtactctccc gctatgcctg ttacctggta gtacaaaacg gcgaccccgc gaaaccggtc
     9601 attgcggcag ggcagactta ttttactatc cagacccgac ggcaggagct tgctgacgat
     9661 gaagcattta agcaacttcg tgaagacgaa aagcgtctgt ttctgagaaa tgagttgaaa
     9721 gaacataaca aacaattggt tgaggcagca cagcaagctg gggtagcgac agctactgat
     9781 ttcgccatat ttcagaatca tggttaccag gggctgtatg gtggattaga tcagaaagcc
     9841 atccaccaac tgaaggggct gaaaaagagt cagaagatcc tggatcatat gggttcaaca
     9901 gaactggcgg ctaatctctt tcgagctacc caaacagaag aaaaactcaa gcgggatagc
     9961 gtgaattcaa aacagcaagc taataccaca cactttgacg tgggtagcaa ggtgaggcaa
    10021 accattcagg aacttggcgg aaccatacct gaggagttgc cgaccccgca ggtcagcatc
    10081 aagcagttgg aaaacagtgt aaaaattaca gagaagaaat agcagaaacg aaaaactctt
    10141 tttgttgatt gagacacccg atgcgtaagc caaggtccag gtgcagtgaa cacaatggct
    10201 aaacattgca cctttctttt cccccctcag ttttaaccta ttttttctta tgcattttct
    10261 cagacaagaa gtcagaagaa tgcatctacg ctacagaaaa tagcgatttc acataactaa
    10321 aagttatcaa tttcctctca ccttaaaaaa tatcaatcgt gacaatgcgc acaaatcgct
    10381 accctgccag acagattttt agggagagaa ccatgctgtt acacattttg tatttggttg
    10441 gcattactgc cgaagccatg acgggggcgc tggcggccgg gcgacggcgc atggatacat
    10501 ttggcgtaat tattattgca accgccaccg caattggcgg agggtcagtg cgcgatattc
    10561 tgctgggcca ctatccgctc ggctgggtca aacacccgga atatgtgatt atcgtcgcta
    10621 ccgccgcagt actcaccact atcgttgctc ctgtaatgcc ttacctgcgc aaagtgtttc
    10681 tggtgctcga tgcgctcgga ctggtggtct tttctatcat cggcgcacag gttgcactgg
    10741 atatggggca cggcccaatt attgccgttg tcgcggcggt aaccaccggt gtatttggcg
    10801 gcgttttgcg cgatatgttc tgtaaacgca ttccgctggt ctttcaaaaa gagttgtatg
    10861 ccggtgtctc atttgcctcc gccgtgctgt acatcgcgct gcaacactat gtttctaacc
    10921 atgatgtggt gattatctcc accctggtat tcggcttttt tgctcgttta ctggcgctac
    10981 gtcttaagct gggattaccg gtcttttact acagccacga aggtcactaa ggttcaaaac
    11041 ctgtgatctg ctgggcagcc agccaactgc ccagcttctt gatttgcgca ttttccttcc
    11101 attcaataac ctgtctggcg cgcccagatc cagtccccgg cagctgctgc cagaactgct
    11161 ccgtgctaaa taaaagttgc gaccaggacc gttcatcact ggcattaagc gccgcccggg
    11221 ttagcggtat tcccattgcc atcacccagc gagtaaaagg ctggttacga gccagattaa
    11281 actgatgcca taactgcgca cttttacttt tcgcgatccc cggcgtgttc tgtaattgct
    11341 ctggcgttaa taaaagccag gaaaagatat gctcaaagcg atgagtctga tgcagcgcgc
    11401 gccaaccggc ctcaccaatg ccatccagcc ccagaacctg ttttgacccc agccagacta
    11461 agcgtgaaat gaactgttcc tgacaaacat cagaagcaaa gtagcaggtc aacgggttaa
    11521 agcggttttc tggtggtgtc ggttttgtac gttctgcacc gcgccagacc acatcatcaa
    11581 tgcgaggaat accctgaccg gcaaggctga cgaggatctg atcaccaggc gcaatatccc
    11641 actcctgcca gcgcctgacg gaaccaatat tcacccgctg gactttttta tcatccagca
    11701 tgacaggtgc gagtgacgca accaccgata ttttaccgct cttacccacc gcaaactgaa
    11761 tcgccttcac ttcggcaacc tgagctacag gttgatattt ccaggccacc agccactccg
    11821 cctggcccgg tagccaatga cgggattcgg gttccttagc ccctcgcaca accacgccat
    11881 cggtgacgaa gggtaatttc gctttccacc actcattgcg tacgcgcgca acctcatcag
    11941 catttttaac cgcacgggta tacctctgcg ttagagtaaa acctgcggta gccagctcct
    12001 ttaaacgatc ggtcattaac tgcggtccat ccggccatgc ccagacaaaa acagccagtg
    12061 agtttagcgt gtcgctattc ccctggcgca tcatcaggcc agcaactttt gcgcgggcat
    12121 ttattccccc catttgttgt tggatatgcc cctcgcgctg gagaaatatt tccccctgaa
    12181 gcgtactgtt ggctaaaggc ccgctaacgg tttgcagcac agcggaaatt aagctaactt
    12241 tctgcgtcca gtcctcgcct ttcaggccat taccgcgact gattgctttg ttcagtttcc
    12301 cgtcccgata aaccagggtt accgcaacgc catcaacttt tggctgcacc caaagatcgc
    12361 tacgttctcg catccacaga cttaatgcgt ttttatccgc cattttgcgt acacccgtat
    12421 gggcaaccgg atgcataacc gcaccattta aaggcggcat catcacgtcg cggggctcgc
    12481 tcacaaagca gcgttgccac tgcgtaagac gggcgcttaa ctgatcgtaa acaccgtctt
    12541 ccacctcact ttttccttcc ttccagtagt catcgtccca ctgttttatt tgctgttgca
    12601 ggcgggaaat ttcttcctgt gctctggctg gcgaccaggc cggacagacc gcccacacag
    12661 atgattgcca gcacaagata cttattaata tcgccatcca tactttcatc ttcacctccg
    12721 ctgtagtcag gctggcagat atacaacgtg gtgaaggcaa agccgagtgg caaaaacgga
    12781 gtctgcgagg acgcttcctg agaatcgtct ttattgcagt aaatcacagg caaatgcgga
    12841 aacagctacg caaaacgcaa caactttgcg caaaaagtgt gagcaagggc tacgtcacat
    12901 ggccgcgccg tgtataataa gctcgtatgt aggctttatt tcgctaatca catacgaaag
    12961 atactcatgg ctcaaggcac gctttatatt gtttctgccc ccagtggcgc gggtaaatcc
    13021 agcctgattc aggctttatt aaaaacccaa ccgttgtatg acacccaggt ttctgtttca
    13081 cacaccacac gccaaccgcg tcctggtgaa gtccacggtg aacattattt ctttgttaat
    13141 catgatgaat ttaaagaaat gattagcaga gatgcgttcc tcgaacacgc agaagttttt
    13201 ggtaattact atggcacttc gcgtgaggcc attgagcaag tactggcgac cggtgtcgat
    13261 gtttttctcg atatcgactg gcagggcgcg cagcaaattc gccagaagat gccgcacgcg
    13321 cggagtatct ttattttacc gccgtccaaa attgaactgg accgccgtct acgcggtcgc
    13381 ggtcaggaca gcgaagaggt cattgcaaag cgtatggcgc aagctgttgc agaaatgagc
    13441 cattacgccg aatatgatta tctgattgtg aatgatgact tcgataccgc gttgaccgat
    13501 ttgaagacca ttattcgcgc cgaacgtctg cgcatgagcc gccaaaagca gcgtcatgac
    13561 gctttaatca gcaaattgtt ggcagactga acctgatttc agtatcatgc ccagtcattt
    13621 cttcacctgt ggagcttttt aagtatggca cgcgtaactg ttcaggacgc tgtagagaaa
    13681 attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta
    13741 ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat cgcgctgcgc
    13801 gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg ccaggaacag
    13861 caagagctgg aagccgctga attacaagcc gttaccgcta ttgctgaagg tcgtcgttaa
    13921 tcacaaagcg ggtcgccctt gtatctgttt gaaagcctga atcaactgat tcaaacctac
    13981 ctgccggaag accaaatcaa gcgtctgcgg caggcgtatc tcgttgcacg tgatgctcac
    14041 gaggggcaaa cacgttcaag cggtgaaccc tatatcacgc acccggtagc ggttgcctgc
    14101 attctggccg agatgaaact cgactatgaa acgctgatgg cggcgctgct gcatgacgtg
    14161 attgaagata ctcccgccac ctaccaggat atggaacagc tttttggtaa aagcgtcgcc
    14221 gagctggtag agggggtgtc gaaacttgat aaactcaagt tccgcgataa gaaagaggcg
    14281 caggccgaaa actttcgcaa gatgattatg gcgatggtgc aggatatccg cgtcatcctc
    14341 atcaaacttg ccgaccgtac ccacaacatg cgcacgctgg gctcacttcg cccggacaaa
    14401 cgtcgccgca tcgcccgtga aactctcgaa atttacagcc cgctggcgca ccgtttaggt
    14461 atccaccaca ttaaaaccga actcgaagag ctgggttttg aggcgctgta tcccaaccgt
    14521 tatcgcgtaa tcaaagaagt ggtgaaagcc gcgcgcggca accgtaaaga gatgatccaa
    14581 aaaatcctct cggaaatcga agggcgtttg caggaagcgg gaattccctg ccgcgtcagt
    14641 ggtcgcgaga agcatcttta ttcgatttac tgcaaaatgg tgctcaaaga gcagcgtttt
    14701 cactcaatca tggacatcta cgctttccgc gtgatcgtca atgattccga cacctgttat
    14761 cgcgtgctcg ggcaaatgca tagcctgtac aagccgcgtc cgggtcgcgt gaaagactac
    14821 atcgccattc caaaagcgaa cggctatcag tctttgcaca cctcgatgat cggcccgcac
    14881 ggtgtgccgg ttgaggtcca gatccgtacc gaagatatgg atcagatggc ggagatgggt
    14941 gttgccgcgc actgggctta taaagagcac ggcgaaacca gcaccaccgc gcaaatccgc
    15001 gcccagcgct ggatgcaaag cctgctggag ctgcaacaga gcgccggtag ttcgtttgaa
    15061 tttatcgaga gcgttaaatc cgatctcttc ccggatgaga tttacgtttt cacaccggaa
    15121 gggcgaattg tcgagctgcc tgccggtgca acgcccgtcg acttcgctta tgcagtgcat
    15181 accgatatcg gtcatgcctg cgtgggcgca cgcgttgacc gccagcctta cccgctgtcg
    15241 cagccgctta ccagcggtca aaccgttgaa atcattaccg caccgggcgc tcgcccgaat
    15301 gccgcgtggc tgaactttgt cgttagctca aaagcgcgcg ccaaaattcg tcagttgctg
    15361 aaaaacctca agcgtgatga ttctgtaagc ctgggccgtc gtctgctcaa ccatgctttg
    15421 ggtggtagcc gtaagctgaa tgaaatcccg caggaaaata ttcagcgcga gctggatcgc
    15481 atgaagctgg caacgcttga cgatctgctg gcagaaatcg gacttggtaa cgcaatgagc
    15541 gtggtggtcg cgaaaaatct gcaacatggg gacgcctcca ttccaccggc aacccaaagc
    15601 cacggacatc tgcccattaa aggtgccgat ggcgtactga tcacctttgc gaaatgctgc
    15661 cgccctattc ctggcgaccc gattatcgcc cacgtcagcc ccggtaaagg tctggtgatc
    15721 caccatgaat cctgccgtaa tatccgtggc taccagaaag agccagagaa gtttatggct
    15781 gtggaatggg ataaagagac ggcgcaggag ttcatcaccg aaatcaaggt ggagatgttc
    15841 aatcatcagg gcgcgctggc aaacctgacg gcggcaatta acaccacgac ttcgaatatt
    15901 caaagtttga atacggaaga gaaagatggt cgcgtctaca gcgcctttat tcgtctgacc
    15961 gctcgtgacc gtgtgcatct ggcgaatatc atgcgcaaaa tccgcgtgat gccagacgtg
    16021 attaaagtca cccgaaaccg aaattaatgt tttatgaacc caacacgtta tgcacgcatc
    16081 tgcgaaatgc tcgccaggcg gcagcctgat ctgaccgtct gcatggagca ggtccacaaa
    16141 cctcataacg tttctgcgat tattcgtacc gcagatgccg ttggcgtaca tgaagttcac
    16201 gccgtctggc ctggtagccg catgcgcacc atggcttcgg cagcggcggg tagtaacagc
    16261 tgggtacagg tgaaaacaca ccgcaccatt ggcgatgccg tcgctcatct caaaggccag
    16321 ggcatgcaga ttctggcaac ccatctttct gataatgctg tcgatttccg cgaaattgat
    16381 tacactcgcc cgacctgcat tttgatggga caggagaaaa cgggcatcac gcaggaagca
    16441 ttggccctgg cggatcagga catcatcatt ccgatgatcg gcatggtgca gtcgctgaat
    16501 gtttccgttg cctcagccct cattctttac gaagcccagc gtcagcggca aaatgcaggc
    16561 atgtacctgc gtgaaaacag catgttgccg gaagcagagc aacaacgcct gttgtttgaa
    16621 ggcggctatc cggtgctggc gaaagtcgca aaacgcaaag gcctgcctta tccccacgtc
    16681 aatcagcaag gcgagatcga agctgatgcc gactggtggg ctactatgca ggctgcaggg
    16741 taagtgccat gaaaggtcgc ctgttagatg ctgtcccact cagttcccta acgggcgttg
    16801 gcgcagcact tagtaacaaa ctggcgaaaa tcaacctgca taccgtgcag gatctactct
    16861 tacaccttcc cctgcgctac gaagatcgca cccatctcta ccccatcgga gaactactgc
    16921 cgggcgttta tgccacggtg gaaggcgaag tgctgaactg caatatctcc ttcggcggtc
    16981 ggcggatgat gacctgccag atcagcgacg gttccggcat cctcaccatg cgctttttca
    17041 atttcagcgc ggcaatgaaa aatagcctgg cgacgggccg ccgtgtactg gcttatggcg
    17101 aagcaaagcg cggtaaatat ggtgcggaga tgatccaccc ggaataccgc gtgcagggcg
    17161 atctcagcac gccagaatta caggaaacgc tcacgccggt ttatccaaca acggaaggcg
    17221 taaagcaggc cacgctgcgt aaattaaccg accaggcgct ggatctgctc gacacctgcg
    17281 ccattgaaga actcctgccg ccggaactgt cacaaggaat gatgacgcta ccggaagcgt
    17341 tgcgcacttt gcaccgcccg ccaccgacgc tacagcttag cgatctggaa accgggcagc
    17401 atccggcgca acgtcgtctg attctggaag aactgctggc gcacaacctc agcatgttag
    17461 ccttacgtgc cggagcacag cgttttcatg cccagccgct gagcgccaat gacacgctga
    17521 aaaataaact cctcgccgcc ttaccgttca agccaacggg cgcacaggca cgcgtagtgg
    17581 cggagatcga gcatgatatg gcgctggatg tgccgatgat gcgcctggta cagggcgatg
    17641 taggttccgg taaaacgctg gtcgccgccc tcgccgcgct gcgtgcgatt gcccacggca
    17701 aacaggtggc actgatggca ccaaccgaat tactcgccga gcagcacgcc aataacttcc
    17761 gcaactggtt tgcaccgctc ggtatcgaag tgggctggct cgccggtaag cagaaaggca
    17821 aagcgcgact gtctcagcag gaagccatcg ccagcggtca ggtgcagatg attgtcggta
    17881 cacacgccat cttccaggaa caggtgcagt ttaacggcct ggcgctggtg attatcgacg
    17941 aacagcatcg ttttggcgtg catcagcgtc tggcattgtg ggagaaaggc cagcagcagg
    18001 gcttccatcc gcatcagttg atcatgaccg ccacgccgat cccccgcacg ctggcaatga
    18061 cggcgtatgc cgatctcgat acctcggtga tagatgagct gccgccaggc cgcacgccag
    18121 tgactacggt cgctattcct gatacccgcc gtaccgacat cattgaccgc gtgcgccacg
    18181 cctgcataac tgaaggtcgt caggcgtact gggtttgtac gttgattgaa gagtcagagt
    18241 tactggaagc gcaggcggcg gaagctacct gggaagagtt gaaactggcg ctaccagaat
    18301 tgaatgttgg cctggtacac gggcggatga aaccagccga gaaacaggcg gtgatggcgt
    18361 cgtttaaaca aggtgagcta catctgctgg ttgccactac cgttattgaa gtcggtgttg
    18421 atgtgcctaa cgccagcctg atgattatcg aaaaccctga gcgtctgggt ctggcgcagt
    18481 tacaccagct gcgcgggcgc gtaggtcgtg gcgcggtggc ttctcactgc gtgctgctct
    18541 acaaaacgcc gctttctaaa acggcgcaaa ttcgcctgca agtgctgcgc gacagtaacg
    18601 acggttttgt gattgcgcaa aaagatctgg agattcgcgg ccctggcgaa ttgttaggca
    18661 cgcgtcagac gggtaatgct gaatttaaag tggcggattt actgcgcgat caggcgatga
    18721 tcccggaagt tcagcgcctg gcgcgacata ttcacgaacg ttacccacaa caggcaaaag
    18781 ccttgataga acgctggatg ccggagacag aacgttactc gaatgcgtaa aaggcggcgg
    18841 tgagaagacc gccgcttcat cggttaaccg gcaaaaatcg gcaacattaa atacagctta
    18901 atcaccagcg cattgacgat atcgataaag aacgcaccga ccatcggcac caccaaaaac
    18961 gccatgtgcg acgggccaaa gcgttcagtg atcgcctgca tgttggcgat tgccgttggc
    19021 gttgcaccga ggccaaaacc acagtgaccc gcagccagca ctgccgcatc gtagtttttg
    19081 cccatcatgc gccaggtaac gaagatggca tacaacgcca tgaagatggt ctgtaccacc
    19141 agaatcgcca gcatcggcag cgccagcgaa gccagctccc acagtttcag ccccatcaac
    19201 gccatcgcca ggaacaacga caagcttacg ttacccagca cggataccgc acgctcaaag
    19261 acgcggtaaa agcccattat tgacagaccg ttgctcagaa tcacgccaac aaacagtaca
    19321 cagacgaagg tcggcagttc aaaagcagtg ccagccaaaa gttgcgcaac aattttcccc
    19381 accgtcaggc agatagcaat cagcgcgata gtttcaatca gcaccaacga ggtgatcatg
    19441 cgtcccacat ccggcttttc aaacgccgtc gggacttcct ggtcatccgg aataccgttc
    19501 ggcgtggtgg agtgtttcac cagatagcgc gccaccggac cgccaatcaa gccgcccagc
    19561 accagaccga acgttgcaca ggccatcgcc acttccgtcg cattggtgaa gccataacgt
    19621 tcaatgaaca atttactcca cgcagcgccc gtaccgtgac cgccggaaag cgtaatagaa
    19681 ccggccaaca gccccatcag cggatcaagc cctaacaggc tagccatacc aatgccaatg
    19741 gcattttgca tcaccaacag accaacaacc acaatcaaga agatgccaac cacacgccca
    19801 ccggcacgca aactggcaat gttggcgttc aggccaatgg tggcgaagaa agccagcatt
    19861 aacggatcgc gcagggacat atcaaagttg acttcccagc ccatgctttt tttcagtact
    19921 agtagcgcca gcgccaccaa caaaccaccc gcaacaggtt ccggtatggt gtatttcttc
    19981 aaaaaggaga cggaatggac caacttacgc ccgagcagca acgtcagcgt tgcggcaaca
    20041 agcgttgcta aagtatcgag atgaaacata gttactcctt tgtatccgca tcactctttc
    20101 atactcgtca tacttcatcg cgcagtggca acttcatgaa ctatgacggg tatcgagtta
    20161 tgctttcctg ggtcaaattc tcagctgtta atgaacaaat ggcatggatt ttaagcagaa
    20221 aaataccaaa agttatataa aaaagtgcat ttatctcatt taaatattta gatgaggatg
    20281 tggcaaacgt ttgcttttct ctttcggtca gctaaaatgc ccgctttgct accacgggat
    20341 tgttttcgat gtctgtttcc accctcgagt cagaaaatgc gcaaccggtt gctcagactc
    20401 aaaacagcga actgatttac cgtcttgaag atcgtccgcc gcttcctcaa accctgtttg
    20461 ccgcctgtca gcatctgctg gcgatgttcg ttgcggtgat cacgccagcg ctattaatct
    20521 gccaggcgct gggtttaccg gcacaagaca cgcaacacat tattagtatg tcgctgtttg
    20581 cctccggtgt ggcatcgatt attcaaatta aggcctgggg tccggttggc tccgggctgt
    20641 tgtctattca gggcaccagc ttcaactttg ttgccccgct gattatgggc ggtaccgcgc
    20701 tgaaaaccgg tggtgctgat gttcctacca tgatggcggc tttgttcggc acgttgatgc
    20761 tggcaagttg caccgagatg gtgatctccc gcgttctgca tctggcgcgc cgcattatta
    20821 cgccgctggt ttctggcgtt gtggtgatga ttatcggcct gtcgctaatt caggtggggc
    20881 tgacctccat tggcggcggt tacgcagcca tgagcgataa caccttcggc gcaccgaaaa
    20941 atctgctgct ggcaggcgtg gttttagcct taattatcct gcttaaccgt caacgtaacc
    21001 cttacttacg cgtggcctca ctggtaattg cgatggcggc cggatatgcg ctggcgtggt
    21061 ttatgggcat gttgccagaa agcaacgaac cgatgacgca agaactgatt atggtgccaa
    21121 cgccgctcta ttacggtctt ggcattgaat ggagtctgct gctgccgctg atgctggtct
    21181 ttatgatcac ttcgctggaa accattggcg atatcacggc gacctctgac gtttccgaac
    21241 agccagtgtc cggtccgctg tacatgaaac gcctgaaagg cggcgtgctg gcaaacggcc
    21301 tgaactcgtt tgtttcggcg gtgtttaaca ccttcccgaa ctcctgcttc gggcaaaaca
    21361 acggagtgat ccagttgact ggtgttgcca gccgctatgt cggttttgtc gtcgcgctga
    21421 tgttgatcgt gctgggtctg ttcccggcag tgagcggttt tgtacaacac attccagaac
    21481 cggttctggg cggcgcaacg cttgtaatgt ttggcaccat cgccgcctcc ggtgtgcgta
    21541 tcgtttctcg tgagccgctg aaccgtcggg cgattctgat tatcgcgctg tcgctggcgg
    21601 ttggtctggg cgtgtctcag cagccgctga ttttgcagtt tgcccctgaa tggctgaaaa
    21661 acctgctctc ctccgggatc gccgcgggcg gtattactgc catcgtgctg aatctgattt
    21721 tcccaccaga aaaacagtaa ttcactggcg gcggtaagat aattaccgcc gcagtctcac
    21781 ctctttcacc attcccgcct tgaggattgc gcgtaaatcg tgcataactc ccttatgtgc
    21841 atttcacggg atggaagacc atgaaattta ttgggaagct gcttctctac attctcatcg
    21901 ctctgttagt ggtgatcgct ggcctctatt ttcttctgca aacccgctgg ggagcagaac
    21961 atatcagcgc atgggtttcc gagaatagcg actatcatct ggccttcggg gcgatggatc
    22021 accgtttttc cgcgccatct catatcgtgc tggagaatgt cacgtttggt cgtgatggtc
    22081 agcccgcgcc cctggtggca aaaagtgtcg acattgcgct aagcagtcgc caactgaccg
    22141 aaccacgcca tgtcgatacc atcctgctgg aaaacgggac gctgaatctc accgaccaga
    22201 ccgcgccgct accgttcaaa gccgatcgtc tgcaactgcg tgatatggcg tttaatagcc
    22261 cgaatagcga atggaaactg agcgcgcagc gggtaaatgg cggcgtggtt ccgtggtcac
    22321 cagaagccgg taaagtgctg ggtacgaagg cgcagattca gtttagtgcc ggatcgcttt
    22381 cgctcaatga tgttcctgcc accaatgtac tgattgaagg cagtattgat aacgatcgcg
    22441 ttacgctgac taacctgggt gccgacatcg cccgcgggac attaaccgga aacgcgcagc
    22501 gtaacgccga cggcagctgg caagtggaaa atctgcgcat ggcggatatc cgtctacaaa
    22561 gcgaaaaatc gctaaccgac ttctttgcgc cattacgctc tgtcccgtcg ttgcagattg
    22621 gtcgcttgga agtcatcgat gctcgtctgc aaggtccgga ctgggcggtg accgacctcg
    22681 atctcagctt gcgcaacatg accttcagta aagatgactg gcagacacag gaaggcaaac
    22741 tgtcgatgaa cgccagcgag ttcatttatg gttcgctgca tttatttgac ccgattataa
    22801 acgcggaatt ttccccgcag ggcgtagcgc tgcgccagtt caccagccgc tgggaagggg
    22861 gtatggtcag aacgtcaggg aactggctgc gtgacggtaa aacgttgatc cttgatgatg
    22921 cggcaattgc cgggctggaa tataccttgc cgaaaaactg gcaacagttg tggatggaaa
    22981 cgacacccgg ttggttaaac agcctgcaac tgaagagatt tagcgccagc cgcaatctga
    23041 tcattgatat cgaccctgac ttcccgtggc agctcaccgc gctcgatggt tacggtgcca
    23101 acctgacgct ggttaccgat cataaatggg gcgtctggag tggctcggcg aatctgaatg
    23161 ccgccgccgc gacattcaat cgtgttgatg ttcgtcgccc gtcgctggcg ctgaccgcca
    23221 acagcagcac ggtgaatatc agcgaactga gtgcatttac tgaaaaaggc attctggaag
    23281 ccaccgcaag tgtttcacaa acgccacaac gtcagaccca tatcagcctg aatggacgcg
    23341 gtgtgccggt gaatattttg caacaatggg gatggcctga attaccgttg actggcgacg
    23401 gcaatattca gcttaccgcc agtggagata ttcaggccaa tgccccgctg aaacctacgg
    23461 ttagcgggca attgcatgcc gtgaacgccg caaagcagca agtgactcaa accatgaatg
    23521 cgggcgtcgt ttccagcggt gaagttacat cgacggagcc ggtgcggtaa acatcaatct
    23581 cgccgggtac gactcagacg tacccggcat tccgtcaata gattacaacg taattgtcag
    23641 cgcattccct tgaggcgtca ccaccagccc ctgctcactt tcagcctgcg aaccgccttg
    23701 cagaccattt actttcacaa cattgcgcaa gcacagcgtc cagttcttcg cctcgcccgc
    23761 accagtcaca gtaatcgtgt tgccagtacg tgctgctttt aaagtaaaga tcaccgatcc
    23821 gtcagcagca ggcacttcac agacggcttc atgcccgtct tgcagattaa agaggtggaa
    23881 tgccgtgcct tcgtgccaca cgtaatcggg acgttgatcg ttgttgccca gcgccagtag
    23941 agtgttatca cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg
    24001 actaccgtcg agttcatcgt tgtgccacag gtgtgtccag cgaccttccg gcaggtagaa
    24061 ctgcacatcg cccgcttcag tgaacaccgg cgcaaccatc acgttgtcgc ctaacatgta
    24121 ttgacggtca aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat
    24181 catcggcgta ccccgcgcgt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg
    24241 gcatttcagt tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg
    24301 cacacgataa gatttgctac cgtgtaaacg gctatggctg gagagcaaac caaacgcgca
    24361 ccagcgtttg taaacgtgcg ccggagcggt attttcaaag ccgccgatat cgtggctcca
    24421 gaagccaaaa cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc
    24481 gtagttagcg taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc
    24541 cgagcgggca aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac
    24601 cagttcgttg tagatgtacg cataatggtt atgcattttc tgcgaatcgg aaccgtcaaa
    24661 ccactgaaca tcaattggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat
    24721 cgcgaccaga cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc
    24781 ataaatcgcc agacctggct gccatttatc ccactgccat agcgaaccgt ccgggcgttt
    24841 gagtaaatag cctttctctt gtaactcttt aaagacgggg gatttttgac cgatataggg
    24901 gttaatccag acgcagattt tcagcccttt cgctttcagg cggcggatca tcccttccgg
    24961 gtcagggaaa gtcagcgggt cccactcaaa atcgcaccac tggaaggctt tcatccagaa
    25021 acagtcaaag tggaaaacat gcagcggcag attgcgttcc gccataccat cgataaagct
    25081 gtttaccgtc gcttcgtcgt agttggtggt aaatgaagtg gttagccaca ggccgaagga
    25141 ccacgggggc ggcagcgccg gacgaccagt aaagcgggta taacgatcaa gtaccgcttt
    25201 cggcgtcggg ccgtcgataa caaagtattc gagatattca ctctcaacgc tgaactgcac
    25261 tttggagact ttctccgatc ccacttcaaa agagacacac tggggatgat tgaccagtac
    25321 cccataacca cggttagtca tgtagaacgg gatattttta tacgcctgtt cagtacttgt
    25381 gccgccgtcc cggttccagg tctctaccgt ctggccattg cgcaccaggg cagtaaagcg
    25441 ctctcccaga ccgtaaactg tttcgccaac gccaagatca agccgctcaa acatataatt
    25501 gcgttgatta ttcgtgtcct gcacgtagcc attatttttc acctgactac cggtaatacg
    25561 ttcgccgttg cgcagaaaat ccagtgacca gaactcacct ttgctgacac gcgcgcttaa
    25621 gttgccactt ttaaactcag cataacgttc tgtgttttcg attgtgacct tcacgtcctg
    25681 caaaatattg agcggataat gaggaccgtt attcagcgcc ccctgaaaat gctcaatccg
    25741 cacaccgaca ataccttcct gtggggagaa aaagcgcagc gtaaataaag gcgtatcaag
    25801 ctgccaggta cgttcacgca catcacgggg ggcagcatag accaccattt cattatcctg
    25861 ctgttcaacc tcgaatacct gaagcgggtg aatcaaattg aggccaggtt gaatcaacca
    25921 gtttccatcg ctaattttca ttgccgttcc ttagttctgc acttcttgag aggtgaattg
    25981 ttgctggcaa cggcgtttac cctgagccag ctgttccata acggttttca gattgtgcgt
    26041 cgtgagtgag tagtagcgtt tagcgataat cgcgctcagc aaataacaga tcgccggaac
    26101 aatcgtgaat agcgcaataa tgatgctaat cgtggcgctg ttctgcgctt tttctgccgc
    26161 atcatatccg ccataagcca gcatccagcc gataagagcg ccgccgaagg ccaaccccag
    26221 tttgagcaca aacagcgtgc cagcaaaact gatcccggtc aggcgtttac cattgcacca
    26281 ctcgccgtag tcgacggtat cgaacatcat tacccactgg ataggtgtca ccagttgatg
    26341 caacacacca atcacgaaga tgaagacaaa catagtgatg ctggcctgca tgggaacaaa
    26401 gaacatcgcg aggctaatca ctgccagcag ggcgttcgtc caccagaaga tagtgacttt
    26461 acatttccag tcggtcagag gttttgccag tgcggaacca atcaggttac ccacgcaata
    26521 agtggtgaga aaagcgacaa acacttccgg cgtgcccaaa atccatgtga cgtaatacat
    26581 catcgcccca ccgcgtacgc acaccgccag gatattgaaa atggttagta aaccgacaat
    26641 ccgccactgg tcgttttgcc agatatcacg taaatcttcc cgcatagacg ttgttgtagg
    26701 tggtgcttca acgcgttctt tagtggtgaa gaaacaaaat gccagcatca tgaatgccac
    26761 cacggaaagg accacgatac cgccctggaa accgagtggt ttattatcac cgccaattaa
    26821 attaaccagt ggcatcatca gaacagtaga aagcatgcct cccgcggtcg ccagcacaaa
    26881 acgccaggat tgcagcgaga tacgctgagt cgggtcattg gtgattacac cacccaatgc
    26941 gcagtaaggg atattgacga cggtataaag taaggtaagt tagggtgtaa gtaattgctg
    27001 catagatcat tttgccgttc atactgagat ctggcgtgct ataggccagt acacagacga
    27061 tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa tttaccccag cgagagcgcg
    27121 ttcgatcggc caacaacccc atgcaaggat cggaaatcgc atccagtgca cgagcgacca
    27181 aaaacatggt tccgacaaat ccggcaggaa tgccaaaaat atcggtataa aagaacatca
    27241 tatataacat tacgttatcg aaaataatgt ggctggcggc gtctcccatg ccataaccaa
    27301 ttttctcttt aacggacaac acttcactcc tcatctgctt ttccttgatg ctataggggt
    27361 gctgagaccg ataacgcatt ttgtaaacca tccctgtgag agcgaatatt ccgatatctg
    27421 gttattaatt tatgattctt gttttatgtg atcgtggtag cgttaattcc gctcatatat
    27481 cattgtaaaa tatgggtttt atataaatta taatgctttc gtgataatac gctgcgttta
    27541 ttaggcggaa aaaactgatc tgggggatgt agaaactcaa ggaagtagct ataatgcgcc
    27601 ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc tccggtgagg cggctggact
    27661 tcaaatccag ttggggccgc cagcggtccc gggcaggttc gactcctgtg atcttccgcc
    27721 agtttacctc ttctgaactc tcccaatgtc aataaatctc ttctaatccc ttgtatatca
    27781 gtatttattt gtatcctgag gtcaacctac ttcaccccgt atcaagtttc ttttgggggt
    27841 actttagggg gtgttatttc ctattgaaat acaagcaccc ccataatagg aaaaatgtga
    27901 tacataacaa cggcttttaa caatatcttt gttatacaag gggtgtattg ggggtatcag
    27961 gttagttact ggcagaaatc cccctcagga acatgaattc atcaaaagcc ggaggatgtc
    28021 atggcattaa cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caggggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc aaaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggcgcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaaccc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttc
//
LOCUS       DAJVPT010000038        32273 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVPT010000038 DAJVPT010000000
VERSION     DAJVPT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..255
                     /locus_tag="OQM47_001731"
     CDS             <1..255
                     /locus_tag="OQM47_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCS1479918.1"
                     /translation="LSRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPA
                     NMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(295..693)
                     /gene="yciA"
                     /locus_tag="OQM47_001732"
     CDS             complement(295..693)
                     /gene="yciA"
                     /locus_tag="OQM47_001732"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1479919.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(798..1337)
                     /locus_tag="OQM47_001733"
     CDS             complement(798..1337)
                     /locus_tag="OQM47_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1479920.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1367..2110)
                     /locus_tag="OQM47_001734"
     CDS             complement(1367..2110)
                     /locus_tag="OQM47_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1479921.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(2138..2227)
                     /locus_tag="OQM47_001735"
                     /pseudo
     CDS             complement(2138..2227)
                     /locus_tag="OQM47_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2467..3105
                     /gene="ompW"
                     /locus_tag="OQM47_001736"
     CDS             2467..3105
                     /gene="ompW"
                     /locus_tag="OQM47_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1479922.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(3165..3671)
                     /locus_tag="OQM47_001737"
     CDS             complement(3165..3671)
                     /locus_tag="OQM47_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1479923.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(3717..4217)
                     /locus_tag="OQM47_001738"
     CDS             complement(3717..4217)
                     /locus_tag="OQM47_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1479924.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(4303..4482)
                     /locus_tag="OQM47_001739"
     CDS             complement(4303..4482)
                     /locus_tag="OQM47_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1479925.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(4862..5668)
                     /gene="trpA"
                     /locus_tag="OQM47_001740"
     CDS             complement(4862..5668)
                     /gene="trpA"
                     /locus_tag="OQM47_001740"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1479926.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5668..6861)
                     /gene="trpB"
                     /locus_tag="OQM47_001741"
     CDS             complement(5668..6861)
                     /gene="trpB"
                     /locus_tag="OQM47_001741"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1479927.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(6873..8234)
                     /gene="trpCF"
                     /locus_tag="OQM47_001742"
     CDS             complement(6873..8234)
                     /gene="trpCF"
                     /locus_tag="OQM47_001742"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1479928.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(8235..9830)
                     /gene="trpD"
                     /locus_tag="OQM47_001743"
     CDS             complement(8235..9830)
                     /gene="trpD"
                     /locus_tag="OQM47_001743"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1479929.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(9830..11392)
                     /gene="trpE"
                     /locus_tag="OQM47_001744"
     CDS             complement(9830..11392)
                     /gene="trpE"
                     /locus_tag="OQM47_001744"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1479930.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(11414..11507)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(11484..11528)
                     /gene="trpL"
                     /locus_tag="OQM47_001745"
     CDS             complement(11484..11528)
                     /gene="trpL"
                     /locus_tag="OQM47_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1479931.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            11666..12547
                     /gene="yciV"
                     /locus_tag="OQM47_001746"
     CDS             11666..12547
                     /gene="yciV"
                     /locus_tag="OQM47_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1479932.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            12544..13164
                     /locus_tag="OQM47_001747"
     CDS             12544..13164
                     /locus_tag="OQM47_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1479933.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            13265..14137
                     /gene="rluB"
                     /locus_tag="OQM47_001748"
     CDS             13265..14137
                     /gene="rluB"
                     /locus_tag="OQM47_001748"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1479934.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(14177..14767)
                     /gene="cobO"
                     /locus_tag="OQM47_001749"
     CDS             complement(14177..14767)
                     /gene="cobO"
                     /locus_tag="OQM47_001749"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1479935.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(14764..15522)
                     /locus_tag="OQM47_001750"
     CDS             complement(14764..15522)
                     /locus_tag="OQM47_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1479936.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            15742..16791
                     /gene="sohB"
                     /locus_tag="OQM47_001751"
     CDS             15742..16791
                     /gene="sohB"
                     /locus_tag="OQM47_001751"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1479937.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(16827..17078)
                     /locus_tag="OQM47_001752"
     CDS             complement(16827..17078)
                     /locus_tag="OQM47_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1479938.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            17458..20055
                     /gene="topA"
                     /locus_tag="OQM47_001753"
     CDS             17458..20055
                     /gene="topA"
                     /locus_tag="OQM47_001753"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1479939.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            20265..21239
                     /gene="cysB"
                     /locus_tag="OQM47_001754"
     CDS             20265..21239
                     /gene="cysB"
                     /locus_tag="OQM47_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1479940.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            21534..21698
                     /locus_tag="OQM47_001755"
     CDS             21534..21698
                     /locus_tag="OQM47_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1479941.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            21701..21868
                     /locus_tag="OQM47_001756"
     CDS             21701..21868
                     /locus_tag="OQM47_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479942.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            21982..22077
                     /locus_tag="OQM47_001757"
     CDS             21982..22077
                     /locus_tag="OQM47_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479943.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            22241..24916
                     /gene="acnA"
                     /locus_tag="OQM47_001758"
     CDS             22241..24916
                     /gene="acnA"
                     /locus_tag="OQM47_001758"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1479944.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(24980..25570)
                     /gene="ribA"
                     /locus_tag="OQM47_001759"
     CDS             complement(24980..25570)
                     /gene="ribA"
                     /locus_tag="OQM47_001759"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1479945.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            25740..26504
                     /gene="pgpB"
                     /locus_tag="OQM47_001760"
     CDS             25740..26504
                     /gene="pgpB"
                     /locus_tag="OQM47_001760"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1479946.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            26653..26961
                     /gene="lapA"
                     /locus_tag="OQM47_001761"
     CDS             26653..26961
                     /gene="lapA"
                     /locus_tag="OQM47_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1479947.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            26968..28137
                     /gene="lapB"
                     /locus_tag="OQM47_001762"
     CDS             26968..28137
                     /gene="lapB"
                     /locus_tag="OQM47_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1479948.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            28330..29067
                     /gene="pyrF"
                     /locus_tag="OQM47_001763"
     CDS             28330..29067
                     /gene="pyrF"
                     /locus_tag="OQM47_001763"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1479949.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            29067..29393
                     /gene="yciH"
                     /locus_tag="OQM47_001764"
     CDS             29067..29393
                     /gene="yciH"
                     /locus_tag="OQM47_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1479950.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29519..29737)
                     /gene="osmB"
                     /locus_tag="OQM47_001765"
     CDS             complement(29519..29737)
                     /gene="osmB"
                     /locus_tag="OQM47_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1479951.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30006..30755)
                     /locus_tag="OQM47_001766"
     CDS             complement(30006..30755)
                     /locus_tag="OQM47_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1479952.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(30845..31018)
                     /locus_tag="OQM47_001767"
     CDS             complement(30845..31018)
                     /locus_tag="OQM47_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479953.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(31166..32273)
                     /locus_tag="OQM47_001768"
                     /pseudo
     CDS             complement(31166..32273)
                     /locus_tag="OQM47_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         8133 a         7828 c         8290 g         8022 t
ORIGIN      
        1 ttaagccgta atcagccgca gtatccggca cgagcacagg cattgcgcat tgaagggcag
       61 gttaaagtta aatttgacgt cacgccggat ggtcgcgtgg ataacgtaca aatcctctca
      121 gccaagcctg cgaacatgtt tgagcgtgag gtgaaaaatg cgatgcgcag atggcgttat
      181 gagccgggta agccaggcag tgggattgtg gtgaatatcc tgtttaaaat taacggcacc
      241 accgaaattc agtaagcaga aagtcaaaag cctccgaccg gaggcttttg actattactc
      301 aacaggtaag gcgcgaggtt ttccttcagg atcaaccgct acatacttaa ataatgcttc
      361 tgtcgcttta tagcgttgcc cgattggttc agacgctact tttttcaccc acacttcaat
      421 attaatgctg accgatgtcg tccctttctg gacacagcgt gcatagcagc acaccacatc
      481 gccgaccgca accggccgta agaaagtcat tccttcaacc cgcacagtca ctacgcgacc
      541 gtgggcaatt tcttttgcca gaatagcgcc gccaatatcc atttgtgaca ttaaccaacc
      601 accaaagatg tcaccattgg cattggtatc ggcgggcatg gctaaagtac gtaaaacaag
      661 atcgccctga gggacgttat gtgttgtaga catggtaaaa ccgacttaaa aggaaaatca
      721 caggcgcgat gctactatga ttttcctgag gagaacagag gagatacagc gccagccccg
      781 aaggactggc agtctggtta ggatttatct tcctgcggca tgtggcggta gatatagata
      841 ccgcttaaca atgtgaagat taaggtaagg gcggtcaggc caaagacttt aaagttgacc
      901 caaatatttt gcggcagcca gaatgcgatg tagatgtttg ccagaccgca aaggataaag
      961 aaaacagccc aggccagatt tagcttcgac cataccggtt gcggcagcgt gagttcttta
     1021 ctcagcatcc gctgaattag cggctttttc atcacccatt ggctgactaa cagggctccc
     1081 gcaaacagag cataaatgac tgtaaccttc catttaataa actcatcatt gtggaagaac
     1141 agcgtcaagc caccgaagac gaccaccaga acaaaagtga tcagggccat cttctcaacc
     1201 ttacgaaagc gaacccagct atagataagc acaatcgccg tggcgacgat cagcgctgca
     1261 gtagccgcat agatgtcata aatcttgtaa aacgcgaaaa agacaaccag cggtaaaaaa
     1321 tcaagaaact gcttcatttt acgattccgt aatcaaatgc ttaaggttat tggcgaatca
     1381 acatatacag gcggaacaga tagatgagca atatggcaga aatcaggttg ctcaaggtgt
     1441 tcgccagtac ggcaccaatt tccggggtta atgcggcaaa agaagaggca aaaagcagca
     1501 acagtgtttt tgccagcaac cagctcagta ctgcgggtgc caccagacgc atattcgccc
     1561 aagtcagccg catactgcta cgcatcgagg caaaaatgcc cattttgtcc tgtaccagca
     1621 tcaccggtgc cagggccagt aaaatggcca tgataattcc cggaacgacc accagcatga
     1681 tgccaatctg tactaaaagg gtagtcagaa aaatcagaat aaataacttg ggcaatatcg
     1741 gcgcactggc accaatagct cgcagcgcac tgactctctg acccgcagac accagctgga
     1801 taattaatat tacgcctccg gcgagaatgg cgttaccgat taattctgaa aaagtggacg
     1861 ccgctgaagc ctgcagcaaa atttgttgct gttccggtga catattctga accaggtcga
     1921 acaacccact actgccgcta acgggcacgc cgtcattgag ctgcgcaagc tgtgcatcac
     1981 tgggtgagaa aacatgccct aacaccactg tgataaacgc acatagcaac gataccaaca
     2041 gaatggtcat aaattgatta cggaagaaat ttccggtgtc acggtatacg gactgcgccg
     2101 tgatagacat gcactctcct tgagttttat gcaggtgtta attagcgggc aattgtaccc
     2161 tggttaagcc tcgggtggca gcatcaggtc ttgtatggaa aagcatatct ttgtaaagcg
     2221 gaggtaattg ctggccttca ggcgtgggca aagcgcggca agtggcgatc gagttaatat
     2281 ttgcgtagag aaaatattta aaaattgatt taaatcacat taaccaggat tctcaatgca
     2341 acttctaaat taatccagat caataaatgg tgaattatca tatgtaatgt gatctatgta
     2401 ggatcatttg ttactccaat gtaggtatat tcgtcacgtt tttataacca taacgacgga
     2461 gcggatatga aaaagttaac agtggcggct ttggcagtaa caactcttct ttctggcagt
     2521 gcctttgcgc atgaagcagg cgaatttttt atgcgtgcag gttctgcaac cgtacgtcca
     2581 acagaaggtg ctggtggtac gttaggaagt ctgggtggat tcagcgtgac caataacact
     2641 caactgggct tgacgtttac ttatatggcg accgacaaca ttggtgtgga attactggca
     2701 gcgacgccgt tccgccataa aatcggcacc cgggcgaccg gcgatattgc aaccgttcac
     2761 catctgccgc caacactgat ggcgcagtgg tattttggtg atgccagcag caaattccgt
     2821 ccttacgttg gggccggtat taactacacc accttctttg ataatggatt taacgatcat
     2881 ggcaaagagg cggggctttc cgatctcagt ctgaaagatt cctggggagc tgccgggcag
     2941 gtgggggttg attatctgat taaccgtgac tggttggtta acatgtcagt gtggtacatg
     3001 gatatcgata ccaccgccaa ttataagcta ggcggtgcac agcaacacga tagcgtacgc
     3061 ctcgatccgt gggtgtttat gttctcagca ggatatcgtt tttaattccg cacaaaaacg
     3121 accccgtaat atacggggtc aataaggaca tggtataaag ggtattattt cttcgcttct
     3181 acgccatcag tttcagaacg aattaaaaat ttctcagttg tttgcggaat atgctgaatc
     3241 agccagtcgg ccatttgctt ttcctcattt aaaatcgctt cgatgatcgg aattgagact
     3301 gtatcaccgg cattttttgc tgctgctaat agtgaggtgt aacaggtgat ttcaaattgc
     3361 tcgaagacat atccgccaat agagccttta actatttcat cagaagggaa tataccaccg
     3421 attgactgcc caagcgcagc cattttactc atggaatctt taatgactga acgtgaaatg
     3481 tcattacgat caagaatagt ttccagttga acaatctggt ttttggtttc actaagatgt
     3541 tgttcaatac gagcgcgtag ttcaggataa ttatctatac ggctggccat ggattcaagc
     3601 atagattcgg cttgcttttc cattgcgtgg gcgtcacgta accagtcatg ataatgttca
     3661 atacgattca tgaaaatgtc ctctttataa aatgactaaa agttaaaatt catatttcag
     3721 gctttatttt cggctttctt atttacgtta ttaagggcca gatcagtcag tttgatgtcg
     3781 gtggcctttt cttcttccag ggtttctttc agaagcttcg ctgctttacg gtaacctaat
     3841 tgttcagcca gcgtcgctaa tgtcccgtaa ctggcaatct cataatgctc gactttctgt
     3901 gctgcggcaa tcagtgcggc atcacgcact tcgtttttct cggtactttc gatgacctca
     3961 ttagcttctt caataagacc ttccattgcc acacatttca tgcgcttaat tttcagattt
     4021 gattctgatt ccacaacttg atcaattcgt tcaatctgtc catgagtttc ctcgaggtgc
     4081 gcatgaaaag cctgacttaa tttttcattt gatgttgctc ttgcgagttt tgccagtgcc
     4141 cgggttaatt gtttttctgc gctgtaggta tctgaaagca gttgaataaa tacatcttca
     4201 atggtcttca tattcatatt tttctccagt gaaatcactg cgagccatac ggcccgcaga
     4261 tgcaattatc tttatcagct gatattaata attaacggtg aatcaggatt tgcctgattt
     4321 attaccaccg ctttgttgac cgcctttttt acccgcttca gatgcgcgtt gcggatcatt
     4381 tttaaaatta ccgccgctat gctgaccgcc tttacggcct gcgtcggatg ccttctcacg
     4441 gtcttcggcg aaatttcctg aactaccacg atgttcggcc atgttatttc tcccgttgcg
     4501 ttgcattgtt tcattaatat gagtgttgtg tgtcgacact cattaaaatt agtcactaat
     4561 gagaattagt caaattaagc gcaacgagaa gatagaggga aatatatttt gaggaacatt
     4621 ctggatatat taacaattac ctgaggaata tgtgacttag agaaaattat tgattttact
     4681 ggtgttatgt tgcgggatta atttgtttga ttatattact gttgggcgga aaaatgacgt
     4741 aagttgacgt tcgaccgggg taagcgaaac ggtaaaaaga taaatattaa atgaatttag
     4801 gatttttccg gcttcattaa agaaagttaa aatgccgcca gcggaactgg cggctgtggg
     4861 attaactgcg cgtcgccgct ttcatcggtt gtacaaaaac tttcagtgcc gccagcattt
     4921 tctctggctc attaatatgt tgctcgatga ttttaacaat ggccgaacca gaaatcgcgc
     4981 ccgcagctcc tgcatcaatc gctgctttta cctgatccgg ggcggaaata ccaaacccct
     5041 gcaatggagg tgcagcgttg tactctttca gcttcgcaac cagatgattg aggggtaacg
     5101 cggcgcggtt ttctgcgccg gtcacgcccg ctcgtgacag caaatatgtg taaccacgac
     5161 cgtaagaggc tatctggcgc agcaggtcgt catcggcatt tggcgggcag atgaagatag
     5221 gtgcgacatt atggcgcaac gcggcctggc ggaagggcgc ggactcttca actggcacat
     5281 cggcaaccag caccgaatcg acgccgactt tctcgcactg ggcataaaac tcatcaatgc
     5341 ctttgttaaa caccagattg gcatacatca acaggccaat gggaatggtc gggtgtttct
     5401 ggcgaatcag tgccagcatt tcaaaacatt gtgccggagt cacacctgcc gcaaaggcgc
     5461 gcagagtggc gttttgaatg gtcgggccat ccgccagtgg gtcggagaag gggataccta
     5521 actccagcgc gtcagcaccg gcttcaatta gcgtatcgat aattttcaat gactgctcaa
     5581 tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc ttctttgcgc tccttcaact
     5641 gggcaaacag agattcgtag cgttccatca gatttcccct cgtgctttca aaatatcgtg
     5701 aacggtgaag atgtctttat cgccgcgacc ggaaaggtta accaccagta gctgctcttt
     5761 atccgggttt tcgcgcatca ttttcagcgc atgggccagg gcgtgggagg attccagcgc
     5821 cgggatgatc ccttcgtgca ggcacagcgt tttgaaggct tcaagggctt catcatcggt
     5881 aatagacacg taatcagcgc gtccagtgct gttaagatac gcgtgttgtg gaccgacaga
     5941 cgggaaatcc agtccggcgg agatggagta agattcttca atctgcccgt cttcggtttg
     6001 catcatcggc gctttcatac cgaaataaat gcccacgcga ccatgtttta gcggtgcgcc
     6061 gtgctcgcca gtttcgatac cgtgaccacc aggctccaca ccaatcaggc cgacgttggt
     6121 ttcattgatg aaatcagcaa acatgccgat ggcattcgaa ccgccgccaa cacaggcgat
     6181 aacggcatcc ggcaggcgac cttctctttc cagaatctgc gctttggttt cttcgccaat
     6241 catccgctga aactcacgga caatggtcgg ataaggatgc gggccagccg cggtgcccag
     6301 catatagtgc gcggtttcat aactgccgga ccagtcgcgt agcgcctcat tacaggcatc
     6361 tttcagcgtg gcggaaccgc tatgtaccgg gatcacttcc gcacccatta agcgcatacg
     6421 aaaaacgtta ggcgactggc gttcaacgtc tttggcaccc atataaatac ggcatttcag
     6481 gccgagcagg gcgctggcaa gggccgacgc cacgccatgc tgaccggccc cggtttcggc
     6541 gatgatttcg gttttaccca tccgcttcgc cagcaacgcc tgccccagca cctggttggt
     6601 tttatgtgcg ccgccgtgca gcaaatcttc acgcttgaga tacagcgtgg tgttcgtccc
     6661 ggctgtaatg ttctggcatt tggtcagtgc ggttggacgc ccggcatagt ttttcagcag
     6721 gtcgttgaac tgggcttgaa attcaggatc tttttgcgcg ctgacaaaag cttcttccag
     6781 ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg ccgccaaact caccaaaata
     6841 ggggttaagt aatgttgtca ttgttccttt ccttaatatg cgcgcagcgt ctggaaaacc
     6901 gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact ctacagcaga attaaaatca
     6961 agtccggcgc agccggtttg tgccgcttcc acgcagttat ctgcgcctaa gccccccgcc
     7021 agcagaacgt tgccaagcga ttgaccattt aatagtgacc agtcaaaacg ttgcccgctt
     7081 ccaccctggc cgttgtctaa aacatattta tcaacgtgct gaaactcgcg ggcgggcagg
     7141 gtttcaccga cgcttaatgc tttccagatg gcaacatgtg ctggcagagc ttcacgcagc
     7201 gtatcgatat acagctgatc ttcattgcca tgcagttgca ctgccgccag cgataacacc
     7261 ttagctttgt ccaccacatc ggcaatatcg tgattgcgga acacgccaac atactgcaac
     7321 ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga cgcaacgcgg tgatgtcgca
     7381 acaaaaatca acccaccgta aatcgcgccc gcgtcataag ctgctttagc atcttgccca
     7441 cgcgtcaggc cacatacttt attctcaccc agcaacaccc ggcgcacggc ggcgtgcaaa
     7501 tcgtcatggg ccatcaacgc cgaaccaatc agaaaaccgt tagcgaagtg gcttaactcg
     7561 cgcacctgag cgtaagtatt gatgccggat tcgctgatta ccgttacgtt gtgccccagt
     7621 ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca aatcacgcag atcgcggttg
     7681 ttgatgccaa cgacctttgc ccctaatgca atggcgcgct ccagttcctc ttcattactg
     7741 acttcggtca gcacccccat ctccagactg tgagcgacgg ctgcaagctg gcgatattgg
     7801 tcgtcatcca gtactgaaag cattaataag caggcatcgg cctggtaata gcgcgccaga
     7861 tagatctggt aagggtcgat aatgaagtct ttacataaaa tcggctgcgg ggcgatttgg
     7921 ctgacgatga ggaggaaatc aaagctcccc tgaaaatatt tctcatcagt cagcaccgaa
     7981 attgccgaag cgtaatgttt ataaatggcg gcaatgcgtg ccggatcgaa atcatcacgg
     8041 atcacgcctt ttgacggcga cgctttttta cactccagaa taaacgccgt gcgtgcaccc
     8101 tgtagcgcat cataaaaatg tcgtgtgctc ggctgaacct cattctgaaa actggccagc
     8161 ggttgctgct gtttgcgggt ttctacccaa atcgccttgt ctgcgacgat tttcgctaaa
     8221 acggtttgca tcatttaccc tcgtgccgcc agcgcggtaa ctctgtcgta agcggaacca
     8281 ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca gatcttcatg gccatgcagg
     8341 cgcattaaca tggcgacgtt cgcagcgacg gctgcttcat gggcggcgtc gcctttacct
     8401 tgtaacaagc gtgttaaaat gtcacggttt tcttcaggtg ttccgcctgc cagttgctcc
     8461 tggtggtagg gagtcaggcc aaaatcttca gcggtcaatt gatagctctt aattttgccg
     8521 tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa cttcatccat cccgccgctg
     8581 tgcaccaccg ccgcgcgttg ataccccagc acgcgcaagg tttcggcaat cggcagcacc
     8641 agttccggac tataaacacc aattaacgcc agcggcggat gcgccgggtt aatcaatggc
     8701 cccagcacat tgaacagggt gcgggttttc agttgctggc gaaccggcat cgcgtggcgg
     8761 aatccggtgt gatatttcgg cgcaaagagg aaacatacgc ctaactcgtc cagcgcctgg
     8821 cgcgatttat tggcgttcat atcaagatta ataccgaatg ccgccagcag atccgacgag
     8881 ccggatttac tggagacgct gcggttaccg tgtttcgcca ctttcagccc acaggccgcg
     8941 gcgacaaacg cactggcggt agaaatattg atactgttgc tgccgtcacc gccagtaccg
     9001 acgatatcag caaacagata atccgggcgc gggaacggcg ctgcgttttc cagtagcgcg
     9061 gttgctgccc cggcgatctc gttcgggtgc tcaccgcgaa ttttcatgct caccagcgcc
     9121 gccgccagtt gttccggctt cagctcgcca cgcaccaccg ctgaaaacag ctggtggctt
     9181 tcttgttggc taagcgtctg cgcctgatac agtttttcca gaatcggttg cagcgtgttg
     9241 gctggctcta gtttctgctg cgcccaggcc agcgtttgtt ccagcaggcg agcgccctgg
     9301 gtggtgagaa tggattccgg atggaactgg aatccacaaa cgcgatccgc atcgtgacgt
     9361 actgccatca ccatgccatt aaaatgggcg ttgatggtta aaccggccgg aatgttactg
     9421 ccaaccagcg agtgataacg cgccaccggc agcgggtttg ttaatccggc aaacatcgcc
     9481 tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa tttcgcccgc ctgaccgaca
     9541 tagcccccgt aagcttcgac aattgcctga tgtccgaggc aaatgccaat aatgggcagc
     9601 ttgccacgca agcgggtgag gagttccggc atacaaccgg cttcgctcgg cacaccgggg
     9661 ccaggagaaa gcatcagtac cggattgctc atcgtcgcca ggcgttcaat taaggtctgc
     9721 gccggaatat ggttgcggta aatcaccacg ttatgaccat tgctgcgcaa ctgatctgcc
     9781 aggttgtacg taaaagagtc gatattatcg agcagcagaa tgtcagccat cagaaagtct
     9841 cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc gcgggctttg ttacgggttt
     9901 cgtcggcttc cgactgcgga acagaatcaa ggactacacc agcacccgct tgcacggtgg
     9961 cgataccgtt ttccaccagc gccgagcgga tcacaatgca ggtgtcgaga tcgccatgcg
    10021 cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg acgaccttcc gcctcggcaa
    10081 ttaactgcat agcgcgtact ttcggcgcac cgcttaacgt ccccatattc atacaggcgc
    10141 gataagcgtg cagggcgtca agatcgtgac gcagttcgcc gactacgcga gagacgaggt
    10201 gcatcacata ggaataacgg tcaactttgg tgagatcggc gatgtagcgg ctgccagggg
    10261 tgcaaatgcg tgccagatca ttacgggcga gatcaaccag catcagatgt tcagacagct
    10321 ctttatggtc agtacgcatt tccagttcaa tacggctgtc gagatctctg tccagtgaac
    10381 catcggcgcg acgaccgcgt gggcgtgttc cggcaatcgg gtagatctca atctggcggc
    10441 tggtggcatc atacttgagc gagctttccg gcgacgcgcc aaatagggtg aaatcattat
    10501 cctgcataaa aaacatgtac gggctgggat tactcttttt cagcacgtaa taggccgcca
    10561 gcggtgacgg gcagggcaga gagaaacggc gagatggcac cacctggaaa atttctccag
    10621 cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa ctcttcatcg ctctgattac
    10681 attcacaacg catatgcggc acggaaacca ctggcagcgg cggcgcggct tcggtcagtt
    10741 gctgacgtag ttcgttcagg cgagcagtga gacgttgttt ttcttcttca ttcggagcaa
    10801 acaggctggc ctgaatacgg gtgctttttt tctgatggtc aatcaccatc agcgtttcag
    10861 cgagataaaa acagaaatca gggcagttat tttccgctga cagttgcggt aaatcttcaa
    10921 atcccgccac aaggtcataa gagaacaggc cgccgaagaa cattgcttct cgttcttcct
    10981 tcggtacatt caacagattc tgcaataaac ggaaagcgtc aaaaaccgaa agggagcata
    11041 agcgggcgtc ttcatccagc agtggactga caggggggaa gcgcagcaca cggcagtttg
    11101 gtgattgttc actttccaca cccgcaggca gggcgttatc cagtagtgcc agcagggctt
    11161 cgccattgcc ggaaagtgcc tgaattgtga cggtgtcacc taatgctgta atgcgcagcg
    11221 cactgtctat cagcagcagg ctttttaaat catctttgct gtcgatatct gcggattcca
    11281 gcagcagcgt tgccggacga ttcccacaca actggtgaaa aagcgcagtc gggttgtcgc
    11341 gataagcgcc ttcgcaggtt agcagttcga gagtcggttt ttgtgtttgc attgttattc
    11401 tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg attgctttac
    11461 gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc aacctttcag tacgaaaatt
    11521 gctttcattg tcgataccct ttttacgtga acttgcgtac tagttaactg gttcgatgat
    11581 taattgtcaa cagctcattt cagaatattt gccagacccg ttatgatgtc ggcgcaaaaa
    11641 acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct
    11701 gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc
    11761 agtcgagatg cgcgtcggca ccctggcgat caccgaccat gacaccacag ctgcaatcgc
    11821 gccagcaaga gaggaaattt cacgttcggg actggcgctg aatcttattc ccggcgtgga
    11881 aatttccacg gtctgggaaa atcatgaaat tcatattgtc gggctgaata ttgatattac
    11941 tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc agcgggcgca
    12001 gcaaattgcc gaaagacttg aaaaagcgca aatacctggg gcgctggaag gcgcacaacg
    12061 actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg cgtttccttg tggagtgcgg
    12121 caaagccagt tcaatggcag atgtctttaa aaagtatctg gcgcgcggga aaaccggata
    12181 cgttccgcca cagtggtgta caatagaaca agctattgat gtcattcatc attctggcgg
    12241 taaggcggtg ctagctcatc ctgggcggta caatcttagt gctaaatggc tgaaaagatt
    12301 ggttgcgcat tttgccgaac accacggcga cgcgatggaa gtcgcgcagt gccagcaatc
    12361 gcccaatgaa cgcacccagc tggccacact tgcgcgtcag catcatttat gggcatcaca
    12421 aggatctgat tttcatcagc catgcccgtg gatcgaactg ggtcgtaaac tctggctgcc
    12481 cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg cagaacacca cagagaggga
    12541 attatgagcc agttttttta tattcatcct gataacccgc agcaacgtct gatcaaccag
    12601 gcggtggaga tcgtgcgtaa aggcggggtg attgtctatc caactgattc cggctatgcg
    12661 ctcggctgta aaattgaaga taaaaacgcc atggagcgta tttgtcgtat tcgccagctg
    12721 ccggacggtc acaactttac cctgatgtgt cgcgatcttt ctgaactctc gacctattca
    12781 ttcgtggata acgttgcgtt tcgtttgatg aaaaacaaca cgccgggcaa ctataccttc
    12841 atcctgaagg ggacgaagga agtgccacgc cgtctgttgc aggagaaacg caaaaccatc
    12901 ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc ttgaagcact gggcgaaccg
    12961 atgctttcca cttcgctaat gctgccaggc agcgaattta ccgaatccga tcccgaagaa
    13021 attaaagacc gtctggaaaa acaggtagat ttgattattc acggcggcta tctcgggcag
    13081 aaaccaacaa cggttattga tcttaccgat gatacgccgg tcgtggtgcg tgaaggcgta
    13141 ggtgatgtga agcctttctt ataagtgcgt aactctgtat actacgcggc cttaaaatgg
    13201 cgctatacgg cgccattgat tctcctgttc gacgcctgtg aaggcgacac caaaaggaag
    13261 ctctatgagc gaaaaattac agaaagtgct ggcccgcgct ggccacggct cccgccgtga
    13321 aatcgaatct attattgaag ccggtcgtgt gagtgttgat ggcaaaattg ctaaactcgg
    13381 cgatcgcgtt gaagttaccc ctgggctgaa aattcgtatc gatggtcatc tgatttcggt
    13441 acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg aaggtgagtt
    13501 gtgtacccgt aacgatccgg aagggcgtcc gacggtgttt gaccgtctgc caaagctgcg
    13561 tggcgcacgc tggattgccg taggtcgtct ggacgttaac acctgtggtc tgctgctgtt
    13621 caccaccgat ggtgaactgg caaaccgttt aatgcatcca agccgtgaag ttgaacgtga
    13681 atacgccgtg cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt tgagtcgtgg
    13741 cgtgcaactg gaagatggtc cggcagcttt taaaaccatc aagttcagcg gcggcgaagg
    13801 gatcaaccag tggtacaacg tgactctgac cgaaggccgt aaccgtgaag ttcgtcgtct
    13861 gtgggaagcg gttggtgtgc aggtgagccg cctgatccgt gttcgttacg gtgatatccc
    13921 actgccaaaa ggtctgccac gcggtggctg gacggagctg gacctcgccc agactaacta
    13981 tctgcgcgaa ctggtggagc ttccgccaga aaccagctct aaagtcgctg tagaaaaaga
    14041 ccgtcgtcgc atgaaggcta atcagattcg tcgtgcggtg aaacgtcaca gtcaggtgag
    14101 cggcagtcgt cgttctggcg ggcgtaataa cggttaatca aagtatgccg gacgtcatat
    14161 ccggcatttt tacagattaa taatcaatcc ctatttgcgc cttaacaccg gcatcaaacg
    14221 catgtctgat ggggcgcagc tcacttaccg tatccgccag ttcaagaata tcccgatgac
    14281 agcctcgacc cgtgataatc accgtctgtt gatgtggacg ttcatttaac gcctgcacca
    14341 cttcttccag tggcaaatag tcatacgcca ccatatacgt cagttcatca agcaaaacca
    14401 tatccagtga ggaatcagca agcatccgct ttgcatgttg ccagacttcg cggcaggcgg
    14461 cggtatcaga ctcgcggttt tgtgtatccc aggtaaagcc cgttgccatc acctgaaact
    14521 caacgccatg tggctccagc agattgcgtt cgccattagg ccaggtgcct ttaataaact
    14581 gcacgacgcc cacttttttt ccgtgaccaa ctgcgcgtgt tgccgtacca aatgccgcgg
    14641 tggttttgcc ttttccattg ccggtaaaga cgatgataat accgcgttca tcctgggcct
    14701 gggccacacg agcatctact ttttctttca ctcgctgctg acgctgttgg tagcgttcat
    14761 cactcattgg gaaattcctg gtttacggcc cggttgggcg tcaaaggtca tgccggtttt
    14821 acggcggctg tcatcgccca tcagccagag gtagagcggc atgatatcag cgggtgtttt
    14881 aagtttctgt ggatcttcgg tcgggaaggc gcttgcgcgc attgcggtgc gcgtaccgcc
    14941 tgggttaatg cagttgacgc gcaggcgctg ctgatattca tcggccagta cctgcatcat
    15001 cccttcggtg gcaaatttcg acgctgcata tgcaccccag ttggctcgtc cctgacgtcc
    15061 aacgcttgat gaagtaaaga ccagtgaacc ggcgtccgat ttgagtaata aaggaagcag
    15121 tgcctgggtg agcataaagg tggcattaac gttgatctgc atgacgtcct gccagacctg
    15181 cggattttgt tcgctcattg ggcaaacatc gccgagcaat ccggcattat gcaaaacacc
    15241 atccagacgc ggataattaa cgacaatgcg ctgtgccagt tgttggcaat tttcggacgt
    15301 gcaggtcagc aaatcgagaa taaaccactg tggctgacgc ccagtttctt cgtttatgtg
    15361 gctggctacc tgacgtaatt tttcttcatt acggcccaac agaatcactg tcgcaccata
    15421 gcgtgcatac gtcatcgcgg cttcacgacc aatcccatcg ctggctcccg tcaccaggat
    15481 aatgcgatca ttgagtaaat cttgttttgg ctggtaatgc atggctactc ctcaacgacg
    15541 ttgtctgtcg cgctgccagt gtactttatg actttggggc tttatgcctg aaacagaagg
    15601 gcatttcaat cagcctgggg cgataacacc gcaaaatgga tactttgtca tactttcgct
    15661 gcaataacat ctctgcgaga cggcttaaca tgcctgttgt aaactgtgag ccaaagcgtt
    15721 gtttaaccaa ggtggggact cgtggaattg ttgtctgaat atggtttgtt tttggctaaa
    15781 atcgttaccg ttgtgctagc gattgcggcg attgccgcca ttattgtcaa tgttgctcaa
    15841 cgtaataaac gccagcgtgg cgagttacgg gtcaacaatc tcagcgaaca gtataaggag
    15901 atgaaagaag aactggccgc ggcgctgatg gactcacatc agcaaaaaca gtggcacaaa
    15961 gcgcagaaga aaaagcacaa gcaagaagcg aaagcagcaa aagcgaaagc caaactgggc
    16021 gaggtggcaa ctgacagtaa acctcgcgtc tgggtgctgg attttaaagg cagcatggac
    16081 gcccatgaag tgaactcgct acgtgaagag ataacggcgg tactcgcagc attcaaaccg
    16141 caggatcagg ttgtgctccg tctggaaagc cctggtggca tggtgcatgg ttacgggttg
    16201 gcggcttcgc agctgcagcg tctgcgtgat aaaaacattc ctttaactgt tacggtagac
    16261 aaagtcgctg ccagcggcgg ttacatgatg gcctgtgtgg cggacaaaat tgtttccgca
    16321 ccgtttgcta ttgtgggttc cattggggtg gtggcgcaaa tgcccaactt taaccgcttc
    16381 ctgaaaagca aagatattga tatcgaactg cacaccgccg ggcagtataa gcgcacgctg
    16441 accttgctgg gtgaaaatac cgaagaaggg cgggagaaat tccgcgaaga gctgaacgaa
    16501 acgcatcagt tatttaaaga ttttgtgaag cgtatgcgtc cgtctctgga tattgaacag
    16561 gtggcaacgg gtgaacactg gtacggacaa caggcggtag agaaaggcct ggttgatgaa
    16621 atcaacacca gtgatgaagt tattcttagc ctgatggaag gccgtgaagt ggttaatgta
    16681 cgctatatgc agcgtaaacg actcattgac cgattcaccg gcagcgcggc agagagcgcc
    16741 gatcgattgt tgctacgctg gtggcagcgg ggtcaaaagc cattgatgta aaagacaaac
    16801 gcgaggctaa gacctcgcgt tttgctttaa tcaaccagat gatatttttc tgaaagcaca
    16861 tgggccaggt gtttgaacat attaaacacc gcggtgcttt tggctgttgg caatccttgt
    16921 tcatctaaaa agtagtcgcc ggtaaatacc agcacgccat tacgctgcgt gacgccggtg
    16981 gcttcaatcc ctgcgagcgt atcctcatgc tcacgaatga ttttgttggc ctctttcaac
    17041 agcgtttcgc ggtcgatggg ttgtgtctct ttgttcatta tttactcctt aaacaaggac
    17101 attagtctac gccaggcatg gcttgcagac aaatatacca cgctggtggc aagagcgcct
    17161 tactggcaac tttggatttt gcatgctaat aaagttgcgt atcggatttt atcaggtaca
    17221 gtgtgacgct ttcgtcaatc tggcaataga tttgcttgac attcgaccaa aattccgtcg
    17281 tgctatagcg cctgtaggcc aagacctgtt aactcagtca cctgaatttt cgtgaacaga
    17341 gtcacgacaa ggggttgata tccgcagaga gcgagtccat atcggtaact cgttgccagt
    17401 ggaaggttta tcaacgtgcg acgcattcct ggaagaatca aattaggtaa ggtgaatatg
    17461 ggtaaagctc ttgtcatcgt tgagtccccg gcaaaagcca aaacgatcaa caagtatctg
    17521 ggttgtgact acgtggtgaa atccagcgtc ggtcacatcc gcgatttgcc gaccagtggc
    17581 tcagctgcca aaaagagtgc cgactctacc tccaccaaga cggctaaaaa gcctaaaaag
    17641 gatgaacgtg gcgctctcgt caaccgtatg ggggttgacc cgtggcacaa ttgggaggcg
    17701 cactatgaag tgttgcctgg taaagagaag gtcgtctctg aactgaaaca actggctgaa
    17761 aaagccgacc acatctatct cgcaaccgac cttgaccgcg aaggggaagc cattgcatgg
    17821 cacctgcggg aagtgattgg tggtgatgat gcgcgctata gccgagtggt gtttaacgaa
    17881 attactaaaa acgcgatccg ccaggcattt aacaaaccgg gtgagctgaa tattgatcgt
    17941 gttaatgccc agcaggcgcg tcgctttatg gaccgcgtgg tggggtatat ggtttcgccg
    18001 ctgctgtgga aaaagatcgc tcgtggtctg tctgccggtc gtgtgcagtc ggtggcggtc
    18061 cgcctggtgg tagagcgtga gcgtgaaatt aaagcgttcg taccggaaga gttctgggaa
    18121 gtcgatgcca gcacgactac gccatctggt gaagcgttgg cgttgcaggt gactcatcag
    18181 aatgacaaac cgttccgtcc ggtcaacaaa gaacaaactc aggctgcggt aagtctgctg
    18241 gaaaaagcgc gctacagcgt gctggaacgt gaagacaaac cgacaaccag taaacctggc
    18301 gctcctttta ttacctctac gctgcaacaa gctgccagca cccgtcttgg ttttggcgtg
    18361 aaaaaaacca tgatgatggc gcagcgtttg tatgaagcag gctatatcac ttacatgcgt
    18421 accgactcca ctaacctgag tcaggacgcg gtaaatatgg ttcgcggtta tatcagcgat
    18481 aattttggta agaaatatct gccggaaagt ccgaatcagt acgccagcaa agaaaactca
    18541 caggaagcgc acgaagcgat tcgtccttct gacgtcaatg tgatggcgga atcgctgaag
    18601 gatatggaag cagatgcgca gaaactgtac cagttaatct ggcgtcagtt cgttgcctgc
    18661 cagatgaccc cagcgaaata tgactccacg acgctgaccg ttggtgcggg cgatttccgc
    18721 ctgaaagcac gcggtcgtat tttgcgcttt gatggctgga caaaagtgat gcctgcactg
    18781 cgtaaaggcg atgaagatcg tatcttaccg gcagtcgata aaggcgatgc tctgacgctc
    18841 gttgaactaa caccagccca gcactttacc aagccgccag cccgtttcag tgaagcatcg
    18901 ctggttaaag aactggaaaa acgtggtatc ggtcgtccgt ctacctatgc gtcgatcatt
    18961 tcgaccattc aggatcgtgg ctatgtgcga gtagaaaatc gtcgtttcta tgcggaaaaa
    19021 atgggcgaaa tcgtcaccga tcgccttgaa gaaaatttcc gcgagttaat gaactacgac
    19081 tttaccgcgc agatggaaaa cagcctcgac caggtggcaa atcacgaagc agagtggaaa
    19141 gctgtactgg atcacttctt ctcggatttc acccagcagt tagataaagc tgaaaaagat
    19201 ccggaagagg gtggtatgcg cccgaaccag atggttctga ccagcattga ctgcccgact
    19261 tgtggtcgca aaatggggat tcgcacagcg agtaccgggg tattccttgg ctgttctggc
    19321 tatgcgctgc cgccgaaaga gcgttgcaaa accaccatta acctggtgcc ggaaaacgaa
    19381 gtgctgaacg tgctggaagg cgaagatgct gaaaccaacg cgctgcgcgc aaaacgtcgt
    19441 tgcccgaaat gcggcacggc gatggacagc tatctcatcg atccgaaacg taagttgcat
    19501 gtctgtggta ataacccaac ctgcgacggt tacgagatcg aagagggcga attccgcatt
    19561 aaaggttatg acggcccgat cgttgagtgt gaaaaatgtg gctctgaaat gcacctgaaa
    19621 atggggcgtt tcggtaaata catggcctgc accaacgaag agtgtaaaaa cacacgtaag
    19681 attttacgta acggtgaagt ggcaccaccg aaagaagatc cggtgccatt acctgagctg
    19741 ccgtgcgaaa aatcagatgc ttatttcgtg ctgcgtgacg gtgctgccgg tgtgttcctg
    19801 gctgccaaca ctttcccgaa atcgcgtgaa acgcgtgcgc cactggtgga agagctttat
    19861 cgcttccgcg accgtctgcc ggaaaaactg cgttatctgg ccgatgcgcc acagcaggat
    19921 ccggaaggta ataagaccat ggttcgcttt agccgtaaaa ccaaacagca atatgtctct
    19981 tcggaaaaag acggaaaggc gactggctgg tcagcatttt atgttgatgg caaatgggtt
    20041 gaaggaaaaa aataaccttt aattctgtca ggtttttata aacaaagggt cgcgaaagcg
    20101 gccctttttt attgcatatt attttttctt cacacctata cactaaggct ataaatgata
    20161 tagtggttat agttagcacc ttttttatta ttaaatcgta ttagtcaccc gccaggtgtg
    20221 acgaaaaaac gatgttctga tggcgtctaa gtggatggtt taacatgaaa ttacaacaac
    20281 ttcgctatat tgttgaggtg gtcaatcata acctgaatgt ctcatcaaca gcggaaggac
    20341 tttacacatc acaacccggg atcagtaaac aagtcagaat gctggaagac gagctaggca
    20401 ttcaaatttt ttcccgaagc ggcaagcacc tgacgcaggt aacgccagca ggacaagaaa
    20461 taattcgtat cgctcgcgaa gtcctgtcga aagtcgatgc cataaaatcg gttgccggtg
    20521 agcacacctg gccggataaa ggctcgctgt atatcgccac cacgcatacc caggcacgct
    20581 acgcgttacc aaacgttatc aaaggcttta ttgagcgtta tccccgcgtt tctttgcata
    20641 tgcaccaggg cccgccgaca caaattgctg atgccgtctc taaaggcaat gccgatttcg
    20701 ctatcgccac agaagcgctg catctgtatg aagatttagt gatgttaccg tgctaccact
    20761 ggaatcgggc tattgtagtc actccggatc acccgctggc aggcaaaaaa gccattacca
    20821 ttgaagaact ggcgcaatat ccgttggtga catatacctt cggctttacc ggacgttcag
    20881 aactggatac tgcctttaac cgcgcagggt taacgccgcg tatcgttttc acggcaacgg
    20941 atgctgacgt cattaaaact tacgtccggt tagggctggg ggtaggggtc attgccagca
    21001 tggcggtgga tccggtcgcc gatcccgacc ttgtgcgcgt tgatgctcac gatatcttca
    21061 gccacagtac aaccaaaatt ggttttcgcc gtagtacttt tttgcgcagt tatatgtatg
    21121 atttcattca gcgttttgca ccgcatttaa cgcgtgatgt cgttgatgcg gctgtcgcat
    21181 tgcgctccaa tgaagaaatt gaggtcatgt ttaaagatat aaaactgccg gaaaaataat
    21241 ttcttgcgtt attttcggca ccttttatgt agcgaaggtg ccggaatata ttctcttttg
    21301 ttacttattt ctataccgtg cacaatattt ttatccctca tatttatagg gtaaattacc
    21361 tgtaaagtag tgtctaatta cccgcctcgc aaattccagt tttcaaatat ctatttcagg
    21421 tcaaaagatt gaattattca ctggagacga ttcgtaaatt cgctggattt ttgactaaag
    21481 tttctttagg atttgtctca tcgtatgatt aattacacca atcattcaac gtcttgctga
    21541 taagtgatgg cgattatatg aggttagcaa tgccttctgg aaatcaggaa ccccgccgtg
    21601 atcctgaatt aaaacgtaaa gcctggctgg cggtttttct tggttctgca cttttctggg
    21661 tggttgtcgc actgctgatt tggaaagtgt ggggataact atggtcggtc aggagcaact
    21721 ggagtcgtca ccattatgcc agcatagtga caatgaacca gaaacgaaac gggaatgttc
    21781 cgtcgttatt ccagacgact ggcaactaac atcgcagcag caagccttta tagaactgtt
    21841 tgctgaagat gatcagccga aacaataatt atcatcattc ttattaccca tttttaatga
    21901 attaaagggc ttttaataca ccgcagcaat aacagcttga gttatctcaa cacaaaataa
    21961 taaccgttaa gggtgtagcc tatgatcaac acaaatatga aatattggtc ctggatgggc
    22021 gcgttttctc tgtcgatgct cttctgggcc gaactcctct ggatcattac tcactgatcc
    22081 ttgaccccgc tgcggcgggg ttgtcatttg ctttgccaca aggtttctcc tcttttatca
    22141 atttgggttg ttatcaaatc gttacgcgat gtttgtgtta tctttaatat tcaccctgaa
    22201 gagaatcagg gcttcgcaac cctgtcatta aggaggagct atgtcgtcaa ccctacgaga
    22261 agccagtaag gacacgttgc aggccaaaga taaaacttac cactactaca gcctgccgct
    22321 tgctgctaaa tcactgggcg atatcacccg tctacccaag tcactcaaag ttttgctcga
    22381 aaacctgctg cgctggcagg atggtaactc agttaccgaa gaggatatcc acgcactggc
    22441 gggatggctg aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg caagggtgct
    22501 gatgcaggac tttaccggcg tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt
    22561 taaacgcctc ggtggcgata ctgcaaaggt taacccgctc tcaccggtcg acctggtcat
    22621 tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag gcatttgaag aaaacgtacg
    22681 cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa agcaagcgtt
    22741 cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta acctcgaata
    22801 tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt atccggatac
    22861 actcgttggt actgactcgc acaccactat gatcaacggc cttggcgtgc tggggtgggg
    22921 cgtgggtggg atcgaagcag aagccgccat gttaggccag ccggtttcca tgcttatccc
    22981 ggacgtagtg ggctttaaac ttaccggaaa attacgtgaa ggtattaccg ccacagacct
    23041 ggttctcact gttacccaaa tgctgcgcaa acatggcgtg gtggggaaat tcgtcgaatt
    23101 ttatggtgat ggtctggatt cactaccgtt ggcggatcgc gccaccattg ccaatatgtc
    23161 gccagaatat ggtgccacct gtggcttctt cccaatcgat gcagtaaccc tcgattacat
    23221 gcgtttaagc gggcgcagcg aagatcaggt cgagttggtc gaaaaatatg ccaaagcgca
    23281 gggcatgtgg cgtaacccgg gcgatgaacc gatttttacc agtatgttag aactggatat
    23341 gaatgacgtt gaagcgagcc tggcagggcc aaaacgccca caggatcgcg ttgcactgcc
    23401 cgatgtacca aaagcatttg ccgccagtaa cgaactgaaa gtgaatgcca cgcataaaga
    23461 tcgcctgcca gtcgattatg ttatgaacgg acatcagtat cagttacctg atggcgctgt
    23521 ggtcattgct gcgataacct cgtgcaccaa cacctctaac ccaagtgtgc tgatggccgc
    23581 aggcttgctg gcgaaaaaag ccgtaactct tggcctcaag cggcaaccgt gggtcaaagc
    23641 gtcgttggca ccgggttcga aagtcgtttc tgattatctg gcaaaagcga aactgacacc
    23701 gtatctcgac gaactggggt ttaaccttgt gggatacggt tgtaccacct gtattggtaa
    23761 ctctgggccg ctgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg
    23821 tgcggtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg ttaaaactaa
    23881 ctggctggcc tcgccaccgc tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa
    23941 cctggcttct gagcctatcg gccatgatcg caaaggcgag ccggtttatc tgaaagatat
    24001 ctggccatcg gcacaagaaa ttgcccgtgc ggtagaacaa gtctccacag aaatgttccg
    24061 caaagagtac gcagaagttt ttgaaggcac agcagagtgg aaggaaatta acgtcacacg
    24121 atccgatacc tacggttggc aggaggactc aacctatatt cgcttatcgc ctttctttga
    24181 tgaaatgcag gcaacaccag caccagtgga agatattcac ggtgcgcgga tcctcgcaat
    24241 gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta agcccgacag
    24301 cccagcgggt cgatatctac aaggtcgggg tgttgagcga aaagacttta actcctacgg
    24361 ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg
    24421 taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgg catttacctg acagcgacgt
    24481 agtctctatt tatgatgctg cgatgcgcta taagcaggag caaacgccgc tggcggtgat
    24541 tgccgggaaa gagtatggat caggctcaag ccgtgactgg gcggcaaaag gacctcgtct
    24601 gcttggaatt cgtgtggtga ttgccgaatc gtttgaacga attcaccgtt cgaatttaat
    24661 tggcatgggc atcctgccgc tggaatttcc gcaaggcgtg acgcgtaaaa cgttagggct
    24721 aaccggggaa gagaagattg atattggcga tctgcaaaat ctacaacctg gcgcgacggt
    24781 tccggtgacg cttacgcgcg cggatggtag ccaggaagtc gtaccctgcc gttgtcgtat
    24841 cgacaccgcg acggagttga cctactacca gaacgacggc attttgcatt atgtcattcg
    24901 taatatgttg aagtaacaag tatttgcttg ccggttattt tgcttccggc aagcaaatga
    24961 attacacgat gcaagagggt tatttgttca gcaaatggcc cattttctcg gctttggtat
    25021 cgagataatg ttcgttattg gggttacgac ctacaatcaa tggtacgcgt tcaacaatat
    25081 taatccctgc ttcggtcaga atttcgactt ttttcgggtt attggttaac aagcggactt
    25141 cattgacgcc aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg
    25201 cgaagcctaa ctggtggtta gcctctacgg tatcgtaacc ttgatcctgc agtgcgtaag
    25261 cgcggatttt attcagcaga ccaatgttac gaccttcctg acggtgatac agcaaaatac
    25321 cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc
    25381 gcaagctgaa cagggcgtca ccggtcagac attcggaatg gacgcgcgca agtaccgggg
    25441 tatgcccgga aatatcgcca tagactagcg caacatgatc gtgtccggtt gccagttctt
    25501 caaatcccac catcaggaaa tcgccccatg gggttggcag tttggcttct gccacacgtt
    25561 taagctgcat gaaattctcc agataatgct ggttctgtat tggcttattt tgccataacg
    25621 agaaggggtt cacctaatca cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa
    25681 tttttcttgg aactggcatt ttcagttatg attgtgggac ttatcaaaaa ggagaggcca
    25741 tgcgttcgat tgcccgacgt accgcagtgg gagctgcact attgcttgtc atgccagtag
    25801 ccgtatggat ttctggctgg cgttggcaac ctggagaaca aagttggcta ctaaaagcgg
    25861 ctttttgggt tactgaaact gtcacccagc cctggggcgt cattacacat ttgattttgt
    25921 tcggctggtt tctctggtgt ctgcgttttc gcattaaggc tgcctttgta ttatttgcca
    25981 ttctggcggc ggcaatcctt gttggacaag gcgttaaatc ctggatcaaa gacaaagtac
    26041 aggaaccacg accttttgtt atctggttgg aaaaaacaca tcatattccg gttgatgagt
    26101 tctacacttt aaagcgagca gaacgcggaa atctagtaaa agaacagttg gctgaagaga
    26161 aaaatattcc acaatatttg cgttcacact ggcagaaaga gacggggttt gcctttcctt
    26221 ccggtcacac gatgtttgct gccagttggg cactgctggc cgttggtttg ctgtggccgc
    26281 gtcggcgaac gttaaccatt gctatcttgt tggtctgggc aacgggagtc atgggaagcc
    26341 gcctgctgct cgggatgcat tggccacgcg atctggtggt agctacgttg atttcgtggg
    26401 cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg tgggccatta acaccacctg
    26461 cggaagaaaa tcgcgaaatt gcacaacgag aacaagaaag ttaacgctgg ttgattttcc
    26521 gaatttaggc cttaaatcat caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa
    26581 atggtacttt aaagggctat tgcggtaagt tgaccataat ttattcgctc taaccacata
    26641 acgggaagta atgtgaaata tttactcatt ttcttactgg tgttagcgat cttcgtgatt
    26701 tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt ttaattatct gttagcgcaa
    26761 ggggagtacc gtatttccac attgctggcg gtattgtttg ctgcggggtt tgctatcggt
    26821 tggttgattt gtggcctgtt ctggctgcga gttcgtgttt ccctggcgcg cgctgaacgt
    26881 aaaataaagc gactggaaaa ccagctttca cccgcgactg acgtggctgt agtgccgcac
    26941 tcgtcagcgg cgaaggaata actttctatg ctggagttgt tgtttctgct tttgcctgta
    27001 gccgctgcct atggctggta tatgggccgc agaagtgcgc aacaaaacaa gcaagatgaa
    27061 gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact tcctgcttag taatcaacag
    27121 gataaagcgg tagacctgtt tctcgatatg cttaaagagg atacaggcac cgttgaagcc
    27181 caccttacgc tcggaaacct gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc
    27241 catcagaccc taatggaaag cgcctcgctg acctatgaac agcgtctgtt ggcgattcaa
    27301 caactggggc gtgattacat ggccgccggg ttatacgacc gcgcggaaga catgttcaat
    27361 cagctgaccg atgaaactga cttccgcatt ggcgcgctgc aacagttgct acaaatctac
    27421 caggctacca gtgagtggca gaaagcaatt gatgttgccg aacgcctggt gaagctgggt
    27481 aaagataaac agcgcgtcga aattgcccat ttctactgtg agttagccct gcagcatatg
    27541 gccagcgacg atctcgatcg tgccatgacc ttgctaaaaa aaggggcggc ggcagataaa
    27601 aacagcgccc gcgtatccat catgatggga cgcgtgttta tggcgaaagg agaatacgcc
    27661 aaagccgtcg aaagtttgca acgcgtcata tcccaggaca gagaactggt cagcgaaacg
    27721 ctggaaatgt tgcaaacctg ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc
    27781 ctgcaacgcg cggtggaaga gaacaccggt gccgatgctg aattgatgct tgctgatatc
    27841 atcgaagcgc gcgacggtag tgaggctgca caggtctata ttacacgcca gcttcagcgt
    27901 catccgacca tgcgtgtatt ccataagcta atggattacc acttaaatga agcggaagaa
    27961 gggcgcgcca aagagagtct gatggtgctg cgtgacatgg ttggcgagaa ggtgcgtagt
    28021 aagcctcgtt atagctgcca gaaatgtggt tttaccgcat acaccctcta ctggcattgt
    28081 ccgtcttgtc gtgcctggtc aactattaaa ccgattcgcg gtcttgatgg cctgtaattt
    28141 ttaaaaaaat ccgactttag ttacaacata ctagttattg atgtcccatt gtgctccggc
    28201 aacgacggag cagaaaaagc ctgctagggg aaaaatcgca actgttaatt ttttattttc
    28261 acgggtagaa tgctcgccgt ttacctgttt cgcgccactc ccggtgccca tcatcaagaa
    28321 ggtctggtca tgacgttaac tgcttcatct tcttcccgcg ccgttacgaa ttctcctgtg
    28381 gttgttgccc ttgattatca taattgtgat gacgcgctgt cctttgtcga caagatcgac
    28441 ccacgcgatt gtcgtctgaa ggtcggtaaa gagatgttta cattgtttgg gccacagttt
    28501 gtgcgcgaac ttcaacagcg tggttttgat atctttcttg acctgaaatt ccacgatatc
    28561 cccaacactg cagcgcacgc tgtcgctgct gcagctgact taggcgtgtg gatggtgaat
    28621 gttcatgcct ccggtggggc gcgtatgatg accgcagcgc gtgaggcact ggttccgttt
    28681 ggcaaagatg caccgctttt gattgctgtg acagtgttga ccagcatgga agccagcgac
    28741 ctggccgatc ttggcgtgac actgtcacct gcagattatg cagaacgtct ggcagcactg
    28801 acgcaaaaat gtgaccttga tggtgtggtg tgttctgctc aggaagctgt gcgctttaaa
    28861 caggtattcg gtcaggagtt caaactggtt acaccgggca ttcgtccgca ggggagtgaa
    28921 gctggtgacc agcgccgcat tatgacgcca gaacaggcgt tggcggctgg tgttgattat
    28981 atggtgattg gtcgcccggt aacgcaatcg gtagatccag cgcagacact gaaagcgatc
    29041 aacgcctctt tacagcggag tgcatgatga gtgattccaa cagccgtctg gtctactcaa
    29101 cggaaaccgg gcgtattgat gagcccaaag cggccccagt acgccctaaa ggcgatggag
    29161 cggtgcgtat tcagcgtcag accagtggac gtaaaggtaa gggcgtttgc ctgattaccg
    29221 gtatcgatct cgatgatgcc gaactgacaa aacttgcagc ggaactgaag aaaaaatgcg
    29281 gctgcggcgg agcagttaaa gatggagtta ttgaaatcca gggcgataag cgtgatttat
    29341 taaaatcact acttgaagcg aaagggatga aggtaaaact cgcaggcggt taacataaaa
    29401 agccacggat atatccgtgg ctttcgaata ttttactgtg tgtattattc agttatttcg
    29461 tactgaacag aatcaagcgt attataattg cgagtcgata atcgacattt atttacgatt
    29521 atttaccgac ctggtgacca ataacaccac cgacagctgc accacctaat gtacccaacg
    29581 tactgccatc ggtcagtact gcaccgccta atgcccctgc acccgcgccg attgcggtgt
    29641 tgcggtcccg tttagaccag ttagaacagg cactcagaga cattgccaaa gtaattgcca
    29701 gaacagccgc ggtcattttt ttgctcgtta caaacataat actctctcct gaatttatga
    29761 ttcacggaag taagctctct ataactataa tagctaagaa taagcctggt gaaattattc
    29821 cgtgaaatct gctcgcgcgc agggttatat cacgcaggtg ataattactt cctgttatat
    29881 cgctgataat aattttatat cttgagagtg ttaataacag gtaaatagtc ttaattatca
    29941 accaggaatc atcttagagc ggatgatttg ccaaactgca aatcatccgt agagaaggga
    30001 aatggttaaa tgtcaacgac gtgaacggtg agtttactgt gctcaagatg catcagatcg
    30061 ctggcgcgta ttttcgaatc ggtaatcacg cgattgaatc gctcaacggg accgatggag
    30121 tatgaatgta cagcaccaaa tttcgagctg tcagtcagga ctatcgcttc gcactctttt
    30181 tccagcacgg cattgaccac atcggtgcgc atcatatcgc gaccggtgaa tccagtttca
    30241 ggttgccagc catcaatacc aataaatgct ttgctgaaat gtacctgctg gatgcactgg
    30301 cgtgtcaaag ggccaaccat actttcgctt tttttctggt acacgccacc gagcaaaatg
    30361 acttcacaag gcgcgtcttt cagcaaatgc gcgatgtagc tgctgaccgt gatgatagtg
    30421 acatttttct tctgctcccc cagagtccgg gcgagcaggg cattgctgct gccattttcg
    30481 ataaagatgg tttcgcccgg ttgaaccagt gacgcggcaa actcggcgag ttcacgcttc
    30541 agcgtatagt tgctcatcat acgggtttcg acgtcatcac tatcaagcga aactgcaaag
    30601 ccatgtgcac ggcggaggta actcagtttt tcgagggtgt tgagatcctg gcgaatggta
    30661 acttcagaaa ctccagtggc ttttgccaga tcggttacgc taacctgacc ctggtcaatg
    30721 accatctgta gaatagtttg ttgtcgggaa ttcatcgctg taattcttat aacgttataa
    30781 tactaaataa aaaaatactg acgcgccggg catcctcaaa taggaaaacc tgaaggcacg
    30841 attgttaaat ctcccacggt gtcttcggga tgtctggcgt agattcagca acctggcgca
    30901 agagttctgc tttaagtatc tcgagattat ggatcgcaga gtggtaatcg cctgccacaa
    30961 ggatatctaa cacaatatca atacgttcgg caacgcgctg ggcatcaaat tcagacatca
    31021 aggacatcct tatagcaaaa gggggaaaat gaataatgca aaaattgccg ctaaaagaga
    31081 agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg acgggatata
    31141 gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta taccagcgtt
    31201 cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca ttgatcccgt
    31261 tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc acctgaagat
    31321 tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca ggttgtttgt
    31381 gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag cgcgccagtt
    31441 gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca cctagttggc
    31501 taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa ctctctgtca
    31561 gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt ttcagggcgg
    31621 tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact cgcaggttta
    31681 tgcctttatc ccgccacttt gccacttggc gtacgacatc gagaatcacc caacggccta
    31741 aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt ggaatcaacc
    31801 cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg acacgctagg
    31861 tgattttcga taaccaactg atcgttttcc agtgctttac gcaagttggt atccagccag
    31921 agatattcaa atacccgctg attcatttct ggagtaaaaa cgcaaaattg tcctcgtccg
    31981 ccttccttcg ctgtgtacat tgctgtgtcg gcgtgacgaa taatagtcgc gctgtccgaa
    32041 ccatgttcgg gagagagcga aatacctact gaacagctgg tataaacttc aattaaacca
    32101 atacgaaagg ggaggcgtaa gcgggtcaaa attcgtgatg ccattgcttc cagcgcgctt
    32161 tgtgaggtat tggatgccag taccagaaac tcatcgccac ctggacgcgc caacacctgg
    32221 tcatgttcga gacagcttaa aatagccaat gacacgtcgc gtaataactg gtc
//
LOCUS       DAJVPT010000039        31450 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVPT010000039 DAJVPT010000000
VERSION     DAJVPT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(898..1827)
                     /gene="virB"
                     /locus_tag="OQM47_001769"
     CDS             complement(898..1827)
                     /gene="virB"
                     /locus_tag="OQM47_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1479954.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(2044..2280)
                     /locus_tag="OQM47_001770"
     CDS             complement(2044..2280)
                     /locus_tag="OQM47_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1479955.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(2284..4185)
                     /gene="ipaA"
                     /locus_tag="OQM47_001771"
     CDS             complement(2284..4185)
                     /gene="ipaA"
                     /locus_tag="OQM47_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1479956.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(4194..5192)
                     /gene="ipaD"
                     /locus_tag="OQM47_001772"
     CDS             complement(4194..5192)
                     /gene="ipaD"
                     /locus_tag="OQM47_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1479957.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(5243..6334)
                     /gene="ipaC"
                     /locus_tag="OQM47_001773"
     CDS             complement(5243..6334)
                     /gene="ipaC"
                     /locus_tag="OQM47_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1479958.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(6354..8096)
                     /gene="ipaB"
                     /locus_tag="OQM47_001774"
     CDS             complement(6354..8096)
                     /gene="ipaB"
                     /locus_tag="OQM47_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1479959.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(8102..8569)
                     /gene="ipgC"
                     /locus_tag="OQM47_001775"
     CDS             complement(8102..8569)
                     /gene="ipgC"
                     /locus_tag="OQM47_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1479960.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(8626..9252)
                     /gene="ipgB1"
                     /locus_tag="OQM47_001776"
     CDS             complement(8626..9252)
                     /gene="ipgB1"
                     /locus_tag="OQM47_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1479961.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(9268..9657)
                     /gene="ipgA"
                     /locus_tag="OQM47_001777"
     CDS             complement(9268..9657)
                     /gene="ipgA"
                     /locus_tag="OQM47_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1479962.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(9670..11154)
                     /gene="icsB"
                     /locus_tag="OQM47_001778"
     CDS             complement(9670..11154)
                     /gene="icsB"
                     /locus_tag="OQM47_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1479963.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            11468..13084
                     /locus_tag="OQM47_001779"
     CDS             11468..13084
                     /locus_tag="OQM47_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1479964.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            13094..13456
                     /gene="ipgE"
                     /locus_tag="OQM47_001780"
     CDS             13094..13456
                     /gene="ipgE"
                     /locus_tag="OQM47_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1479965.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            13456..13914
                     /gene="ipgF"
                     /locus_tag="OQM47_001781"
     CDS             13456..13914
                     /gene="ipgF"
                     /locus_tag="OQM47_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1479966.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            13923..15038
                     /gene="mxiG"
                     /locus_tag="OQM47_001782"
     CDS             13923..15038
                     /gene="mxiG"
                     /locus_tag="OQM47_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1479967.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            15046..15297
                     /gene="mxiH"
                     /locus_tag="OQM47_001783"
     CDS             15046..15297
                     /gene="mxiH"
                     /locus_tag="OQM47_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCS1479968.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            15310..15603
                     /gene="mxiI"
                     /locus_tag="OQM47_001784"
     CDS             15310..15603
                     /gene="mxiI"
                     /locus_tag="OQM47_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1479969.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            15609..16334
                     /gene="mxiJ"
                     /locus_tag="OQM47_001785"
     CDS             15609..16334
                     /gene="mxiJ"
                     /locus_tag="OQM47_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1479970.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            16331..16858
                     /locus_tag="OQM47_001786"
     CDS             16331..16858
                     /locus_tag="OQM47_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1479971.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            16800..17495
                     /gene="mxiN"
                     /locus_tag="OQM47_001787"
     CDS             16800..17495
                     /gene="mxiN"
                     /locus_tag="OQM47_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1479972.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            17488..17895
                     /gene="mxiM"
                     /locus_tag="OQM47_001788"
     CDS             17488..17895
                     /gene="mxiM"
                     /locus_tag="OQM47_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1479973.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            17879..18307
                     /gene="mxiM"
                     /locus_tag="OQM47_001789"
     CDS             17879..18307
                     /gene="mxiM"
                     /locus_tag="OQM47_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1479974.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            18437..19069
                     /gene="mxiE"
                     /locus_tag="OQM47_001790"
     CDS             18437..19069
                     /gene="mxiE"
                     /locus_tag="OQM47_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1479975.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            19056..20756
                     /gene="mxiD"
                     /locus_tag="OQM47_001791"
     CDS             19056..20756
                     /gene="mxiD"
                     /locus_tag="OQM47_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1479976.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            20775..21842
                     /gene="mxiC"
                     /locus_tag="OQM47_001792"
     CDS             20775..21842
                     /gene="mxiC"
                     /locus_tag="OQM47_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1479977.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            21855..23915
                     /gene="mxiA"
                     /locus_tag="OQM47_001793"
                     /gene_synonym="virH"
     CDS             21855..23915
                     /gene="mxiA"
                     /locus_tag="OQM47_001793"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1479978.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            23928..24329
                     /gene="spa15"
                     /locus_tag="OQM47_001794"
                     /gene_synonym="spaK"
     CDS             23928..24329
                     /gene="spa15"
                     /locus_tag="OQM47_001794"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1479979.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            24333..25625
                     /gene="spaL"
                     /locus_tag="OQM47_001795"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             24333..25625
                     /gene="spaL"
                     /locus_tag="OQM47_001795"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1479980.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            25718..26056
                     /gene="spa13"
                     /locus_tag="OQM47_001796"
                     /gene_synonym="spaM"
     CDS             25718..26056
                     /gene="spa13"
                     /locus_tag="OQM47_001796"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1479981.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            26043..26921
                     /gene="spa32"
                     /locus_tag="OQM47_001797"
     CDS             26043..26921
                     /gene="spa32"
                     /locus_tag="OQM47_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1479982.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            26915..27796
                     /gene="spa33"
                     /locus_tag="OQM47_001798"
                     /gene_synonym="spaO"
     CDS             26915..27796
                     /gene="spa33"
                     /locus_tag="OQM47_001798"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1479983.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            27801..28451
                     /gene="spa24"
                     /locus_tag="OQM47_001799"
                     /gene_synonym="spaP"
     CDS             27801..28451
                     /gene="spa24"
                     /locus_tag="OQM47_001799"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1479984.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            28466..28726
                     /gene="spaQ"
                     /locus_tag="OQM47_001800"
     CDS             28466..28726
                     /gene="spaQ"
                     /locus_tag="OQM47_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1479985.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            28726..29496
                     /gene="spa29"
                     /locus_tag="OQM47_001801"
                     /gene_synonym="spaR"
     CDS             28726..29496
                     /gene="spa29"
                     /locus_tag="OQM47_001801"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1479986.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            29503..30531
                     /gene="spa40"
                     /locus_tag="OQM47_001802"
                     /gene_synonym="spaS"
     CDS             29503..30531
                     /gene="spa40"
                     /locus_tag="OQM47_001802"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1479987.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            30544..30786
                     /locus_tag="OQM47_001803"
     CDS             30544..30786
                     /locus_tag="OQM47_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1479988.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         9849 a         4543 c         6185 g        10873 t
ORIGIN      
        1 cataacaaat gatattactg agtacaaatt tagctatatt gggcggatta gttgtgtggg
       61 aagacgaaat gtttctcttg aaataagtga actccctcaa ggaatgattg tgtgcttaaa
      121 tatttaagtg ctggtaagtt tgagaaggct gacatatttt gcagagcaaa cagtgattga
      181 cttggatgat atttttattt tagtcactcg ttgtaattgg agtatgcttt attttatttg
      241 aaaatagatg tattttatac ccgcaattta aactgtaagc cctatgaggg gtatggatat
      301 tataagttac tctttgacca agcataactt ttggggttag aaaaataaat ctcttgttaa
      361 taattaatat gattttaaaa tcaaacttgt ttataattat aaaataatgt aagtcggtca
      421 ttttatggga agattaggag aataaaacag taattatatt tgagaaatct gctaatatat
      481 tcttaaagct tttttgatac tgtgaacgaa gtctgttaat ggtggatttt ttgtttatca
      541 ccatgtaaaa aaaattgagt agtaaaagcg ttgactgcta atcctcgctg tttttattga
      601 aacgattaaa aatacgctga atattttcgg ccagtcactc atattcacat aaaattttga
      661 ccatcatgcc gcatccctta gttgcacaat tcattttcaa tgatatgaat attactaatt
      721 tcaccagata attttctcgt ggtcagcagg gtatgagttt tcaccgtaat tttaaggaaa
      781 cacagtgcgt ggggattgaa taacagatag ccgacggaat taccccaccg gctgaaagtc
      841 aagttcaacg aagattttta ttatctgaat tgggcagttt acatcagtgt tcgatgttta
      901 tgaagacgat agatggcgag aaattatatc ccgaatagct tcatctattt ctctctgaac
      961 tgttttgttt attttagata atgtaaatat tagagttctg tttgttttat tctcttttct
     1021 ttttatgtaa gtccgtttgt ctttagaaat aaataacgag tcaactttta gcgaagggtt
     1081 ttgctttctg tttttgcttt tctttatgat gtttaaaatt tcttttttat atatgtccgg
     1141 gggcaaattt gtatcaaggt cttttatttc ttcctttaat attggtagtg tagaactaag
     1201 agattcatta gccttttcaa gtcctttata ataattgaat aatatcttgt agtcattaaa
     1261 gttaagttct gacgcgattg gaaatagaga tattatttcc tgtggaacgc ttgctgcctg
     1321 aaaggcacga gtgactttcg cgcgagacag attctctttt ttggctatgt ctttatagga
     1381 catccctgat actttcagaa aattaagacc aataccaagt tctcggatgc tatgctcttt
     1441 tgctgtttgt atatcgtttg ctagttttct ggcatcaaga gtagatatat attcttttga
     1501 atatagaact tcaagatctg ctcctgcata tattgcagat gctcttctac gagtgccatc
     1561 cagaatttca attctaccat caatctccct tcctattaca gggaagaatt gttgtagctt
     1621 tatagttttt atgatatcgg ctaatgattc ttctgttagg gattcttgat cgcgtacatt
     1681 cgttttttgg ttgacgaagg ttaaatcttg gatcctttta tgaggaatct tggctttgat
     1741 aaaggaaact ttattaccag aatggagtgt aaactctttc ttttggcctt cctttatact
     1801 taacaagtcg ttgcacaaat ccaccatatc acaccctgtt tattcatatt gatttatatt
     1861 gacaaaagtt aaatgcagtg tgagcaagat tgtatgtaac tgttgatgat agtcaacggt
     1921 agtcttaaaa attactattt taatagctgg attgatgcaa tttatcttta ttggataaaa
     1981 gaataatatt cttgtggagc tctgatgtga gagctccata gatgaaaaaa tgcttatcaa
     2041 ttactatatt gattttgtac tatttttgac tacagaaata agatctccaa tagtaataat
     2101 gtgttctaat gtagattcat ctatacgtag attgaactca aaactaatca tatccagcat
     2161 ttcaatcaga gaaagagaat cggcagaaag atcttcaata aatttagttt cttcagatag
     2221 tttgctgtac gctataccat agcaaaaggc cactattgat aatatttttt cttttatcat
     2281 attttaatcc ttattgatat tctttaatac ttttgatagg gaagtggtta catcttttgc
     2341 tgctttatat atattattgt tttcagctgt aatatcattg ttatttttta aatcagatat
     2401 atcatctgta agtaattctg cagaggtatc gtcaattttt gacaatacct tgctgagggc
     2461 gctagatact tcttttgcct tttcatagat cgcatgattt ttatcaattg tatctgcgtt
     2521 ggtttctgtt acgtagtttc ttatatttgc atctgtaact tttaatttgt tgtttattct
     2581 atgttctgtt gtggattttg taatgtcctt tgatttttcc tgtaactctc gttcttgtgt
     2641 tcctgattta gttccaatga gagatataag attacttagg gagtttgtta ctttttttga
     2701 agcttcaaat atcgtttccc gtgttaaata tgtgtctcca tttcttgata aaaaatcagt
     2761 ctgaagtact tttgaatcag tgtttgagtt gaatttattg atttgcgaag ctactctatg
     2821 agaaagagaa gcgctacttt tagttgtatc tgatgagtta tcattactgc ttgtcactcc
     2881 ttccgggtgg agaggggggg tggttgaaga gttctgtatg ttcagaggcc tgtgtccccg
     2941 agaaagagag ttatttgcag atctttcttg actactcgta gttaacaggt ctgaacctat
     3001 tccatccgaa ttatctggag tatggttgaa agccttttct aaagctgaat tatttgcaaa
     3061 tgtctggaca gtatctgata tagagtgtgg tagaacggaa gttacttttt gaactagtgc
     3121 tctttgtgga tttagtaatg agtttccgtt acgggataat agatcgtttg tctgagaagt
     3181 aggagactgt gcatcatttt ctgggttctc gtgatagcta ttgtcatata ctctgttgtc
     3241 aaaaactcta ttatcgtaag ttctattatc attatttata tgatagtgaa ttacaggttg
     3301 ggatgttttc tcattggctc cgggggttgg tccgccatct ggcatatctt cgggaggtgt
     3361 agggaggcca tcttggatag gatgcgaatc ttttatatct tctaaaatat cattaaggac
     3421 ttttctcaac agctcaggaa acacggctac tgtatttaag tctcttgaca gtttacctat
     3481 accaaatcct ttactatcaa tagtattttt acgttgattg cgcaaactat caaaggcaag
     3541 ttttgcctgt gcgtcaacta aatcatctag tttgagagat gaaacagttt cattacttaa
     3601 ctttaattca gcaagaatat tattcttgat aaatgatgat acttcagtat tgaggagttt
     3661 ttgtcccaat gataaagctt caccagatgt gtgttgttgt gctaatagtg caagtctgtg
     3721 ctcaatgttt tgtcgtaatt gatggagttt cttattcttt gccgaccatg gtccaaaggc
     3781 agaatcttgg gataatttat cttttataat tagttccgaa tactgtgcga caattttttg
     3841 aagtcccttt tcgttttcaa aattaagacc attttgatgt actaaggagt acaaaacatt
     3901 tgaaaattcg agaatctcgc ttttggcata cgcattcatt gagttggaat aaatgcttct
     3961 taacacatct tctttccctg aggaagaaaa taaaaaatca agacatttct gattaaaaga
     4021 cgttgcaaag ttttcttttt cagacactga tgctggtgtt tttagagaag tttgcgaact
     4081 atggatatcg gatattttgc tttttaactc gctgtattct gttgatgatg gtgaagttgc
     4141 cttatataag aatgttggcg cttgagtatt atttacatta tgcatggcgc acctcagaaa
     4201 tggagaaaaa gtttatctgt atctgtacat gagcttattg tactactcaa aacctttact
     4261 aaattatcaa aaatactatt ggcattactg tatttttgaa ctaaagtttg aagattattt
     4321 ttcattgttt catcttcggc agagaatccg gcattccatg cctgatattt tgcattatct
     4381 agcacaacct cgccatttcc acctagatta tctaagcttt ttaacatatt gtctattggg
     4441 gtcatgttta tactgacaac atatcccccg tttttttgag ataccttgcc gattgttcca
     4501 cctaattctg taagccattt atttgcttgt tcctgactaa cagtattatt tgctggatat
     4561 agcggtttat ctttatattt ttccttgagt tcttccaatg cctttttaag cgagttgact
     4621 tgtaatttca cggagtttcc gtcgttacct ccgggagaga tccagccggc aagactggaa
     4681 agaacagcgc taaaatcttg atacatttga gtatatgaac taacggcatg ttcatatact
     4741 ttcagatact gttcattaat atcattgatg gagtttgcaa ttttagccca cagttctcta
     4801 tgagatatca tttgatctcc atcaagctcg gcttctttcg gagctgaatg taataattct
     4861 cttgcgtctt tattaattgg atattcgtgc ctggaaagaa tatctaatag ttgagcggat
     4921 ttatttacat ccatgctaat ctgagatgaa tgtaatgcta tttcttctag cgatgtttta
     4981 gtcaacgttt tttgtgaaag ctctttcttt aatgcctgat ttgttgttct gatattatga
     5041 agggtgtcgt tgagcatagt aagggaactt acaggatgag aactggtcgt tgtttttata
     5101 tcagaattaa ctgtttcggt tgatgaaccg ttggtattgt ttggactgaa tgatgaggtg
     5161 gaaatactat tagtcagagt tgttatattc atggttattt ccttacagga agagccatat
     5221 attattatgg ctcttggata tattaagctc gaatgttacc agcaatctga ctggctgtcg
     5281 aattctttga ttggttgatg ctgtcaatta tattcaataa tttttgtatt aattgatttg
     5341 tcgcttggga tgcttcttta gatacttggg atgcttcctc tgcttgttta ctgctggcct
     5401 gactgattag ttgttcttct tcttcaagag cagatgcata acgccctcct gatgtggata
     5461 tattaccagc aacggctgat gtttccattg ttgcacggcc aatgttttgt ttttgctgcg
     5521 cagaaagggt attgagctcg taagttcttc tctgggtgtc aattttatcc tgcaaagaaa
     5581 tatcgggaga actaagagaa gttttatgtt cagttgacag ggatatatta tctggcgcca
     5641 gtttattttt acctaaaaat tttgtgctag agttagtttg tggtgaggtg atatttgtat
     5701 ctatttgttt atttaaccct aatttagaac ctgcaagctc tttttcaaga gattgagcag
     5761 tggcaaggtt ctttcttaag gctccttttt ggtcgctaat ccctgaatgc gtttttttgg
     5821 caccgatacc cgttatacct acttgtgtga ctgctccagt aatgcttgat gatagggctg
     5881 ccaggccttg ccgaacaatg ttctctgcag ctgattttgt agcatcgaac gcaatcaatg
     5941 acaattgaga gcccaattta gtttctgcag tgcggagagc agaaagtaga acggctacac
     6001 taataattaa agaaacagca ttagaagaaa gactggaaat atccagagtg ttctctggag
     6061 taagggtgtg actcatctct gaaagatctt tcatcttttt gtcagtaagg cgagcaatga
     6121 tttctgaatc atgctgaacc tgctctgata actttaaaag ttgatcatca tttaatgtgg
     6181 ttgttaatac gggattttta ccgacattaa gtggaatatg cgctgcaatc tgctgataat
     6241 tttgtgattt ttgtgtttcg gaagaacttt gagtttgttt tgtggatata tctgtatata
     6301 aaatctgggt tggttttgtg ttttgaattt ccataacatt ctccttattt gtatcaagca
     6361 gtagtttgtt gcaaaattgc ttttgcaaca tcagttctat tagcctgaga gttggacatt
     6421 gaggctaata gatctgcaat tacttcctgc aattggccga atttttctat tgcttcactg
     6481 atatattttg acagttgttc aacttgatat ttcgataatg tcaggtctgc tagatttgtc
     6541 gacgcgctgt tctggaaaac agcagaagcg acacttcctc ccgcttgtgt ggcagagtta
     6601 acactttctc ctaataaaac tgcttggttt aaatgggtgg aaataatttg tttggatatt
     6661 acttcatcac ccgctgcacc aagaaattta tttaatctgg caacagcatt agtgattaaa
     6721 tcgtccagtt gagaagaaaa attcttgaga aactttggta taaggtctgt gagggtttta
     6781 cctattattt tgccaatatt ttctgcaagt tttgctgctg cctgtttacc aacagtggct
     6841 acgagaacga ctgctgcaac taggacaaga gcgcctgcga ttgcccccag aatagagcca
     6901 atcattttgg cttttttcga gtcgacgccc aagccttcga gcatttttgt aaatgcatct
     6961 gaaaggagtt tgattaaggg ttcaatgact gctttcatga tcggattcag ggcttgttcc
     7021 atgaaggaat tgccggtcgc tgcttgtact atagcatccg taaccataag agctaaacca
     7081 acagctgcca gtgctagaga ggctcctcca gaaaaagctg ctgcaacaac actaacgata
     7141 gttaaaagtg ccccaagtat tttcccaaca caacccatta ctctgttgag ttcttctgct
     7201 ttacgtactt cagcagcata ctcatcagat tttctctcca tttcagtttt tcttgattct
     7261 tggagagact ggaatagagc cagatcattt tttaaagatt cttcattatt ttttccaact
     7321 agttgaataa aggttgccat caattgagta acactggcaa gtccggttaa tgatttctgc
     7381 tgggttgata gctgttcagc agatgctgtg tttgaaaatg cagaaaaaga gtctatttct
     7441 ttttcgagtt gcattgattt atctgtaagt ttgctatgaa ttgacagggt tttctgctca
     7501 atcaatgtcc tgtctttaac tgctgcgtct tttttgatag tgagttgtat ttcttcccgg
     7561 cttaattttt tcttttctgg tgactctggg tcgagttcgg ataatcttgt ttgaatttgg
     7621 ttaattttat tttctaggtc ttttatttta gaatctgcgt tttttagttt attaatttgt
     7681 ttttcatagt ctctggttag tccttcagtt tcagatagaa gagtgttaat tttatcggag
     7741 aattctaggt ttttttgctg tcttgcctgt tgctgggact tccaagcagt aattttattt
     7801 gttaatgcag ttaaagattt ttcaccgagt atttgaataa ggtttccaat taaaagcgtt
     7861 agttgggaac ttgcatttaa tgactttggt gctttaagtt cagggattaa tatatttgaa
     7921 gttgagtgtg cattagttgt attaatattt tggttagcag taagatcagc aattgtaaga
     7981 gaaaataatt tgttagctgc atcatttgca gcttggatag tattgtctcc aagctcagtg
     8041 gaagccaata ttttggcaag aggaaaacca gtggttgtgg tgcttacatt atgcataata
     8101 attactcctt gatatcctga attgcgtcca agtatgattg tgcttttatt tttaattttt
     8161 catcattgct gtgttgaatt acgagttcga agcactcttt agcttttaag ggggctttca
     8221 accgaagctg gcattgtcca gtatggaata ctggtgtata gtcatttttt cctaatgcaa
     8281 aagcgacagc ataaaggtct gctgcttgtt ggaactgttc ttttatctga taaatagctg
     8341 cgagtcccat aatgtagtct acattgtaaa agtcgtatat acataaaaac ctgaagaaaa
     8401 cttcagcttc ctctattctt cctttgttgt aaaagtcata agcatatgaa taaatgtcat
     8461 ccatcatatc atcaggaatt gcattaatat ctttcagtgt agcgccagag ttaattgcat
     8521 caattactgc agtagagatg ctttcatttt cggtgatatt taaagacata aggtctcctt
     8581 tttgtctgct ttactttaat aagtataaga tttaatataa aagatttaat ttgtattgct
     8641 ttgacggtat acagccttta gaacctctgc gcttatttct atttgtaaaa cttttgttga
     8701 ttcattaaat attttatcgt agacggaact ggatactaga tttgtaagat ccttcatttg
     8761 tgtgttaaac aaatccatgt cactgctata gcgttttaaa atctttgctc tgaggccaga
     8821 tgaggcaatc tcgtttagtg ctgtatgact gatagatgtt tgtgctgatt tttcatcaag
     8881 ttttatatcc gggaattttt tttcaatagc tgcaaatatg ttttttttgt tatctgaggt
     8941 caaaacaact ccattgtgta tggccatttc atctatactt ctgtttatca ttgaagagac
     9001 ataagtggtt cgctcttggc tcatccaaca aaatagttga ttccccgaat tactatcttt
     9061 tattttgtct atgacgctgt ccctgatttt atttatattc accgggcatc caaaattctt
     9121 ctgaggaaag cgttgtttta agttaaaaac agataatatt ttctttacaa gcttattgcg
     9181 acttaacgaa tcaaatgatg ggcgaggaat cgcgaattca acctgtggaa gtattttgtt
     9241 tagaatttgc atgatacccc ctatatgtta gttcacttct gaagtgatgt ttgcaatggt
     9301 tagtttaata caaaataaaa attcatacaa agattcaatg agttcatcag gtgaaagata
     9361 ttttttgtag tgtttcttgg tcaaaacaca ttgatgagta tcagggagaa gacagataga
     9421 agaaccataa tctctactca aagagtagtt aaggatgagt atgtgttctg gattaataat
     9481 attttctgta aaaagttcat gttcattaag aagtccggtc aaaaagatgt attcatcatt
     9541 atctgttata caaacaggta tttgttcttc gagcaatata aaggcttgat ttttatcgtt
     9601 aaaatcaagc tttgcacctg ttatttcata aagtttatca tatagtttgc gacacatttc
     9661 tatggccttc tatatattag aatgagagtt attcaataaa agcttattga tctgtaataa
     9721 gtcgtgaatg taaagtttgg ttccaagatc tggcgattta agagaattgt aataatcaat
     9781 aatttttatt gatttttcga tgatttcttc ttttttatct gttgcttcgg tttttaattc
     9841 gtttaataca cgagtcatat tgctgggtgc tgattttact aagtatgagt ggcaagcgtt
     9901 gatgaccgca tcaaagtctt ctgaagatac attttcagta ggatgtttgt aaagtgaaat
     9961 tgataaaggc ttgaatgttt ttggtgtgtg gataaggtta attttattga aacttttaag
    10021 atagctgcgt cgtaattgag tcaatctgga aagatcaaaa tcagattcaa tatacttcat
    10081 tatttcgtta taagactgat tgagactttc aatgcgttgc ctaactttat ttgcatacgc
    10141 gtatacatcg ttaggtgtag caaccaattt aacgtcaggg agtggaaagt atatttcaga
    10201 atttcctgct ttaagaacat taagtgccat accagcacag ttttcagatt tactgattaa
    10261 tttataattt gccaattgtc tgaaagcatc tgctttcttt ttgcatataa attttgacat
    10321 gtttgtttca tcaaggccaa aaaggttatg gctgatttta ccatcctttg ttttattatc
    10381 acctgataat ggaatgtata ctttatcagc actgaccccc caataaccat aggggtcttt
    10441 tttttgatta gctctgggaa aaaatatttt tttgttgtta ttttgatctt gttttgaatt
    10501 taataagttt ctaacctctt ggcctgcatc aagtctttcg gctgtttggt ctgaaatcat
    10561 atttagtttg tcttgcttat aggtatcgac actgtaacct gagcgttttt caaaaaggta
    10621 gtttatttct gtatcttttg taacattccc gccgggatac cagctgagat aggtatgagt
    10681 tggttcctgt agtaaattgt tttttatact gatactagta tgccctaaat gggttatgcc
    10741 tctatttccc cagcaacgaa aaactaaatg tctttcaaga tttttggctg taccgatgcc
    10801 atgaaaacgc tctgcggtat tcaacattaa tttgatgtct ctacctgtga aagagtgttg
    10861 aattgagttt ttagctgctt cataggcggc aaacgcactt tctgcagatt tttcgctagc
    10921 taatgtattg aggaaaagca ataaggattt ttgattctct agtcgataat tttttagttg
    10981 ttcatttaat atctgtgaat ttatctttgc attaatagtg cttaatgttc tgaaaaagag
    11041 atcctttagg ttgaatttct gtgctatcaa tataggtcca tgctctgtat cttcaacgcg
    11101 tataggccct tttgtattgc ttgcgtcaat gaaattgcta attttgagga tcatacttta
    11161 ttaactctcc attacttggt gattgtgggg atgtaaaatg tatttggtat ataatatatt
    11221 caattaataa atttagaaac ttgagcctgt taacataatc aaattttctt ttgctgtaca
    11281 taatatgtac ctcgtgagca tatgtagtgc tcgtttcatc atgaaatccc acaagataaa
    11341 gtgcctgatg tatcaggctc ggagtgttat agaaaaagag agaaccctgt tgaataagta
    11401 tcttttttgt tgtaaaacga ggtacaaaac tgcaatcaat gccccattta tgactgagga
    11461 taattaaatg cacataacta atttgggatt gcatcaggtt tcatttcaaa gcggagattc
    11521 ctataaaggc gcagaagaaa ccggaaagca caaaggtgta agcgtgattt catatcaaag
    11581 agttaaaaat ggagaaagaa ataaggggat cgaagcgctt aatcgattat atttacagaa
    11641 ccaaacaagc ttaacaggaa aaagcctttt gtttgcgcgt gacagagctg gagttttttg
    11701 cgaggcaata aagttggcag gtggtgatac gtcaaaaatt aaagccatga tggaacgatt
    11761 agatacctat aaacttggtg aagttaataa aagacatatt aatgagctta ataaagtaat
    11821 aagtgaagag ataagagcac agctaggcat taaaaataaa aaggaattgc aaacgaaaat
    11881 aaaacaaata tttactgatt atttaaataa taagaattgg ggaccggtcg acaagaatat
    11941 tagtcatcat gggaaaaatt atggttttca attaactcct gcctctcata tgaagatagg
    12001 gaataaaaat atatttgtca aagagtataa tggaaaagga atttgttgtg cttctacgag
    12061 agagagtgat catatcgcga atatgtggct ttcaaaagtg gtggatgatg aggggaagga
    12121 aattttttcg ggtatcagac atggagttat atcagcctat ggattgaaaa aaaactcatc
    12181 tgaaagagct gttgctgctc gtaataaagc tgaggagtta gtaagcgcag cattatatag
    12241 cagaccagag ttattatcac aggctttgtc tggtaaaaca gtagatttaa agattgtttc
    12301 gacttctctt ctgacgccga ccagtttaac cgggggagag gagagtatgt taaaagatca
    12361 ggtgaacgca ttaaaagggt taaactctaa aagggggaag ccaacgaaat tattaattcg
    12421 gaatagtgat ggtcttctga aagaagtaag cgttaatcta aaagtggtga catttaactt
    12481 tggtgtaaat gaattagcgc taaaaatggg cttaggctgg aggaatgttg acaagcttaa
    12541 tgatgaatca atatgttctt tgttggggga taatttcctc aaaaatggtg tgattggagg
    12601 ttgggctgca gaagcaatag aaaaaaatcc accatgtaag aatgatgtga tatatctggc
    12661 taaccagata aaagagattg taaccaaaaa gttacaaaaa aatgataatg gagagccgta
    12721 taaattgtca caaagaatga ctctattggc ttatactata ggtgctgtac cttgctggaa
    12781 ttgtaagagt gggaaggaca gaacaggtat gcaggatgct gaaattaaaa gagaaataat
    12841 aagaaaacat gaaacaggtc agttttctca attaaatagt aaattatcct cagaagagaa
    12901 aagattattt tctactattc taatgaatag tggtaatatg gaaatccaag agatgaatac
    12961 tggtgtgccc ggaaataaag tcatgaaaaa attgccgcta tcttcgttag agctatctta
    13021 ttctgaaaga ataggggacc caaaaatatg gaatatggtg aaagggtatt cgtcatttgt
    13081 ataagaggaa tatatggaag atttagcaga tgttatttgc cgagccttgg gtatcccttt
    13141 aattgatatc gatgatcaag caatcatgct tgatgatgat gtgctaatat atattgaaaa
    13201 agaaggagat tcaataaatt tattatgccc tttttgtgca ttgcctgaaa atataaatga
    13261 tcttatatat gcgttgagcc taaattactc agaaaagata tgcttagcta ctgatgatga
    13321 aggcggaaat ctaattgcgc gattagatct taccgggata aatgaattcg aggatgtata
    13381 tgttaataca gaatattata tttcgcgtgt gcgttggctg aaggatgaat ttgcgcgaag
    13441 aatgaagggg tattaatgtc ccgtttcgta tttattttgc tgtgttttat cccatattta
    13501 gggagagctg attgttggga taaggctggt gaaaggtaca atattccatc aagtttgtta
    13561 aaagcgattg cggaaaaaga atccggattt aataaatctg cagtcaatgt taataacaat
    13621 ggaagtaaag attatggtat aatgcaaata aatgactttc attctaaaag acttagagaa
    13681 atgggatatt ctgaggaaat gctaattagt catccatgtc tttctgtaca ttatgcagca
    13741 aagttattaa atgagtttat gatgatgtat ggaagagggt gggaggctgt tggagcctat
    13801 aatgcaggaa cgtctccgaa aaagaaaaaa gaaagattaa agtacgcaga ggatatctat
    13861 agacgctatt tgcggatagc agcagagagc aagcagaata atcgaaggat ataagaggat
    13921 ttatgtctga ggcaaagaac tcaaatcttg caccattcag gttattagtg aaactcacaa
    13981 acggtgtcgg ggatgagttt ccgttatatt atgggaataa cttaattgtt ctcggacgga
    14041 cgatagaaac acttgagttt ggaaacgaca atttcccaga aaatattatt cctgtaacag
    14101 atagtaaatc ggatggtata atttatttga ccataagcaa agataatatc tgtcaattta
    14161 gtgatgaaaa aggtgagcaa attgatatca attcacaatt taattcattt gaatatgacg
    14221 ggatttcttt tcaccttaaa aatatgaggg aggataaatc gagaggccat atactaaacg
    14281 gaatgtataa gaaccactcg gtgttttttt tctttgctgt tattgttgtg ttaattataa
    14341 ttttttccct ctctttaaag aaagatgaag taaaagaaat tgctgaaatt atagatgata
    14401 agagatatgg aatagtcaac actggacagt gcaattatat tcttgccgaa acacaaaatg
    14461 atgcggtatg ggcgtctgta gccttaaata aaaccggatt taccaaatgc agatatattc
    14521 tggtgtcaaa caaagaaata aatagaattc aacaatatat caatcaacga ttcccattta
    14581 tcaatttgta tgtgttgaat ttagtgagtg ataaagcgga attactggtg tttttaagta
    14641 aagaaagaaa tagttcaaaa gataccgaac ttgataaatt gaaaaatgct ctgattgttg
    14701 aatttccgta tatcaaaaat attaaattca actatcttag cgatcataat gcaagggggg
    14761 atgctaaagg tattttcaca aaggtaaatg tccagtataa agagatttgt gaaaataata
    14821 aagttacata ttctgtcaga gaagaactga cagatgaaaa gcttgagctc attaatagac
    14881 tgatttctga acataaaaat atatatggag atcagtatat tgagttttct gtacttttga
    14941 tagatgatga ttttaaaggt aaatcatatc ttaacagcaa agacagttat gtaatgttga
    15001 atgataaaca ctggtttttt ttagataaaa ataagtgagg ataaaatgag tgttacagta
    15061 ccgaatgatg attggacatt gagttcatta tctgaaactt ttgatgatgg aactcaaaca
    15121 ttacaaggtg aactaacatt ggcactagat aaattagcta aaaatccttc gaatccacag
    15181 ttgctggctg aataccaaag taaattatct gaatatacat tatataggaa cgcgcaatcc
    15241 aatacagtga aagtgattaa ggatgttgat gctgcaatta ttcaaaactt cagataatag
    15301 ggagcattca tgaattacat ttatccagtc aatcaggttg atattatcaa agccagtgat
    15361 tttcaatctc aagagatatc aagtctggaa gacgtcgtgt cggctaaata tagtgatatt
    15421 aagatggata cagatattca agtatcacaa ataatggaga tggtaagcaa tccagaatca
    15481 ttaaacccag aatctttggc caagttacag acgacgctct caaattattc aataggagta
    15541 tcattagctg gcacgttagc aagaaaaaca gtttcggctg ttgaaacttt attaaagtct
    15601 taatttatat gattaggtat aaaggtttta ttttattctt gttgctgatg ttgattggat
    15661 gtgagcaacg tgaagagtta atttctaatt tatctcaaag acaggcaaat gaaataatat
    15721 ctgtgctaga acgccataat attactgcta gaaaagttga tggaggtaaa caggggatct
    15781 cggtacaagt cgaaaagggg acatttgcat cggcagttga tttgatgcgc atgtacgatt
    15841 tgccaaatcc ggagagagtt gatatctcac aaatgtttcc tacagattca ttagtgtctt
    15901 ctccaagagc tgaaaaggcc cgtttatata gtgctattga gcaacggctg gaacagtctt
    15961 tagtttctat tggtggtgtt atttcggcaa aaatacatgt tagctatgat cttgaagaaa
    16021 aaaatatatc ttcaaaaccg atgcatatat cagtaatcgc tatatatgac tcaccgaaag
    16081 agtctgaact attagttagt aatattaagc gatttttgaa aaacaccttt tctgatgtta
    16141 agtatgaaaa tatatctgtc atattaactc cgaaagaaga atatgtttat acaaatgtac
    16201 aacctgttaa ggaagttaaa tcggaatttt taacaaatga agtaatatat ttatttctcg
    16261 ggatggctgt actagttgtc attcttttgg tatgggcatt caaaacaggg tggttcaaga
    16321 gaaacaaaat atgataagaa tggatggaat ttataaaaaa tatctttcaa taatttttga
    16381 tccagcgttc tatataaata gaaatcggtt gaatttgcct tctgaactgt tagaaaatgg
    16441 cgtaatcaga agtgagatta ataatctcat aattaataaa tatgatctaa attgcgatat
    16501 tgaaccttta agcggggtaa ccgctatgtt tgttgccaac tggaatttac ttccagctgt
    16561 tgcttatttt atcggtagtc aagagtctcg gttaattaat catagtgaga tggttattag
    16621 ttattatggg ggcaaaatca gtaagcaagg ggaggccgct attcgaagtg gtttttggca
    16681 tcttattgcg tggaaggaaa atatatctgt tggtatatat gaaagaataa acttattatt
    16741 taatccaatt gctttggagg gaaattatac gcctgtagaa agaaatttgt cccgattaaa
    16801 tgaaggtatg caatatgcaa aaaggcactt taccggtatc cagacatcat gcctatgatg
    16861 gtgttgttat aaagagaatt gaaaaggaat tatgtaaaac cataaaagat agagacactg
    16921 agagtaagaa gaaagcaatt tgtgttataa aggaggctac gaaaaaagct gaatcactgc
    16981 gtatagatgc tgtatgtgat ggttatcaaa ttggtataca gactgctttt gagcatatta
    17041 ttgactatat ttgtgaatgg aagttaaagc aaaatgaaaa tagaagaaat attgaggact
    17101 atataactag tttgctttca gaaaatttac atgatgagag gatcattagc actttattgg
    17161 agcagtggct tagttcgttg cgtaatacag taacagaatt aaaagtggtt ttgcccaagt
    17221 gtaatttagc actgaggaaa aagttagaac ttgatctcca taaatatcgc tcagatgtca
    17281 agattatttt aaaatattct gaagggaata attatatatt ctgtagtggt aatcaagtag
    17341 tggagtttag tcctcaggat gtaatatcag gtgttaaaat agaacttgcg gaaaaattaa
    17401 caaaaaatga taaaaaatat ttcaaagagt tagcacataa gaaactgaga caaattgcag
    17461 aagacttgtt aaaagagaat cctgttaatg attaatcaaa taaatgcaag caatgctctg
    17521 cagcaaagat taaatagtga agaagttgtt aatttaaatg agagattatc atcttcgcaa
    17581 tcatttgatg aggatataat ctatgaaata atgcaatatt tctcacagag tgaattaaat
    17641 tccatagata atgatgaact gcacaataag attgaacaat tattcaattc acgttttcct
    17701 tatcttacag ctgcacaaaa aagttcactt cttaataaac tcatagatgc aaatcaatat
    17761 gtcgatttgc atgagggatt ttatgcatca ttgtccatat ataacaatat tgatttctat
    17821 attaagacta caaccttcga ttcattaatt agtgtctttg aagcagggag agaggcagat
    17881 gattcgacat ggtagtaata agttgaaaat atttatttta agtatattgc tattaacact
    17941 gagtgggtgt gctttaaagt catcatctaa ttctgaaaaa gaatggcata ttgttcctgt
    18001 aagtaaggat tatttttcta ttccaaatga tttattatgg tcgtttaata caaccaataa
    18061 aagtataaat gtttactcta aatgtattag tggtaaggcg gtttatagtt ttaatgcagg
    18121 taaattcatg ggcaacttta atgttaagga agtagatggg tgcttcatgg atgcacaaaa
    18181 gatagctata gataaactat tttctatgct gaaagacggg gttgttttaa aaggtaataa
    18241 gataaatgat accatcctta tagagaagga tggggaagtt aaattaaaat taattcgagg
    18301 gatataattg tattgtgagt aaatataaag gtctaaatac aagtaatatg ttttacattt
    18361 actctagtgg acatgaacca gttaacgttg agcttgtaaa agataaagaa cgtaacataa
    18421 ttgagctggc tccagcatgg aagggttttt ttttgtccga aatcaaaata taaaattcag
    18481 tgataatgtg aactatcatt atcgctttaa cattaatagt tgtgcaaaat ttcttgcttt
    18541 ttgggactat ttcagtggtg ctctagtaga gcatagccat gctgaaaaat gtattcattt
    18601 ctatcatgag aatgatcttc gggatagttg taataccgaa tcgatgcttg ataagttaat
    18661 gttaagattc atcttttcat ctgaccagaa tgtaagtaat gcactggcta tgatacgaat
    18721 gactgagtca tatcatttgg ttctttatct tctgagaact attgagaaag aaaaggaggt
    18781 aagaataaag agtctgacag agcattatgg tgtgtcggag gcttatttta gatctttatg
    18841 tagaaaagct ttaggggcaa aagtcaaaga acaactgaac acttggcggt tagtaaatgg
    18901 tttattagat gtatttttgc acaatcaaac aatcacttca gctgccatga ataatgggta
    18961 tgcatcaaca tcccattttt caaatgagat aaaaacacga ctaggctttt ctgcaagaga
    19021 attatcgaat attacttttt tagtgaaaaa aataaatgaa aaaatttaat attaaatctt
    19081 tgactctctt gattgtattg ttaccattga tagttaatgc taataatatt gactctcatt
    19141 tattagaaca aaatgacata gcgaaatatg ttgctcaaag tgatacggta ggatcttttt
    19201 ttgaacgttt ttctgcgtta ttaaattatc ctattgttgt tagcaaacaa gctgcaaaaa
    19261 agagaatatc tggggagttt gatttatcta atccagaaga aatgcttgaa aaactgacat
    19321 tacttgtggg actcatatgg tataaagatg gaaatgcatt atacatatat gactctggag
    19381 aattaataag caaagttatt ttgctggaga atatatcact taattatctt attcagtatc
    19441 tgaaggatgc taacctttat gatcacagat atcctataag aggaaacatt agtgataaaa
    19501 cattctatat atctggtcct ccggcattgg ttgaactggt agctaataca gcaactttgc
    19561 ttgataagca ggtttcttct attgggacag ataaagttaa ttttggggtt ataaaattaa
    19621 aaaatacatt tgttagtgac agaacttata atatgagagg ggaggatatt gtcatcccag
    19681 gggtggctac tgttgtagaa cgtttattaa ataatggtaa ggctctgagt aacagacaag
    19741 cccaaaatga tccgatgcca ccctttaata ttacacagaa agtatctgag gatagtaatg
    19801 acttttcatt tagttcagtt accaatagtt ctatattaga ggatgtatca cttattgcat
    19861 atccagaaac caattctata ttagtcaaag gtaatgatca gcaaatacag attattcgcg
    19921 atattatcac tcagttagat atagccaaac gacatataga attatcatta tggataatcg
    19981 atattgataa atctgaatta aataaccttg gtgttaattg gcaaggaacg gcttcatttg
    20041 gtgattcgtt tggtgcgtca tttaatatga gctcatcagc atcgataagc actttggacg
    20101 gcaataagtt tattgcttca gttatggcat taaatcagaa aaagaaagca aatgtagttt
    20161 caaggccggt gattttgaca caagagaata ttccggctat atttgataat aacaggactt
    20221 tttatgtgtc attagttggt gagcgcaatt cttcgttgga gcatgttacg tatggaacat
    20281 tgataaatgt tatacctcgc ttttcatctc ggggacaaat tgagatgtct ttaacgatag
    20341 aggatggaac aggaaatagt cagtcaaatt acaattataa taatgaaaat acatctgtcc
    20401 ttccagaagt tggaaggacc aaaatcagta caatagcgag agttccacaa ggcaaaagtt
    20461 tgttgatagg tggttatacg catgaaacga actctaacga aataatatct attccttttc
    20521 tctcctctat tcctgtaatc ggtaatgtgt tcaagtataa aacaagtaat atcagtaata
    20581 ttgtaagggt ttttctgatt caaccccgtg agataaagga gtcaagttat tataatactg
    20641 cagaatataa aagcctcata tctgaaagag aaatacaaaa gactacacaa ataatcccat
    20701 ctgaaactac tttgctggaa gacgaaaaat cattggtttc atacttaaat tactaactat
    20761 aaagtaggtg atgtatgctt gatgttaaaa atacaggagt ttttagctct gcattcattg
    20821 ataagttgaa tgcaatgaca aattcagatg atggagatga gactgctgat gcagagcttg
    20881 attctggctt ggctaatagc aagtatattg actcatctga tgagatggct tccgctcttt
    20941 cgtcatttat aaacagaaga gaccttgaga aactgaaagg aacaaatagt gatagtcagg
    21001 aacgtatttt agatggggaa gaagatgaaa ttaatcacaa gatttttgat ttaaagagaa
    21061 cgttaaaaga taaccttcct ctagatcggg attttataga cagactaaag agatatttta
    21121 aagatccaag tgatcaagtc ttagcattaa gggaactttt gaatgaaaaa gatcttactg
    21181 ctgaacaagt cgaattatta actaaaatta ttaatgagat aatatcaggt agtgaaaaaa
    21241 gtgttaatgc tggaataaat tcagctatac aggctaaatt atttggcaac aaaatgaaac
    21301 ttgaaccaca gcttttgcgt gcatgttatc gtggttttat catggggaac atatcaacaa
    21361 cagatcagta tatagaatgg cttggtaatt ttggttttaa tcacagacat acaattgtga
    21421 attttgtaga gcagtcactg attgtagaca tggattctga gaaaccgagc tgtaatgctt
    21481 atgagtttgg ttttgtgtta tctaaattaa ttgcaattaa gatgattaga acttcagacg
    21541 taatttttat gaagaaactg gaatcctcaa gcttgctaaa agatggcagt ttaagtgcag
    21601 agcagctatt gctaacttta ttatatattt ttcaatatcc aagtgaaagt gagcaaattc
    21661 ttacttctgt tatagaagta tcacgagcca gtcatgagga ttctgtagtg tatcaaacat
    21721 atctatcttc tgttaatgaa agtcctcatg atatatttaa aagtgaaagt gaaagagaaa
    21781 ttgcgatcaa tattctacga gagcttgtca caagtgcata caagaaagag ctttctagat
    21841 aacaggagat aaaagtgatc cagtcttttc ttaagcaagt aagtactaag cctgaattaa
    21901 ttatattagt gcttatggtt atgatcattg ctatgttgat cataccatta cctacatacc
    21961 ttgttgattt tttaataggg cttaatattg tacttgctat tctcgttttt atgggatcat
    22021 tttatattga aagaatattg agtttttcta cattcccttc ggttttgttg ataacgacat
    22081 tatttcgtct cgctttatca ataagtacta gtcgactaat tttagttgat gcggatgcgg
    22141 gtaaaattat tactacattt ggccagtttg tcattggtga tagtctggct gtaggttttg
    22201 ttattttttc aatagtaact gttgtgcagt tcattgttat aacaaaagga tctgaaaggg
    22261 ttgcggaggt agctgctcgc ttttctcttg acggtatgcc ggggaaacaa atgagcattg
    22321 atgcggattt aaaggctgga attattgatg ccgcaggagc taaagaaaga cgcagtattc
    22381 tggaacgtga gagtcaattg tatggttcat ttgatggagc aatgaagttc attaaaggtg
    22441 atgcaattgc tggcatcatt attatttttg tcaatttaat aggaggtatt tctgttggta
    22501 tgagtcaaca tggaatgtcc ctatctggtg cattatctac ttataccata ctaactattg
    22561 gtgacggatt ggtctctcag attcctgctc tgttaatttc tataagtgca ggatttatcg
    22621 ttacaagagt taatggtgat agcgataata tgggacgtaa cataatgtcc cagatatttg
    22681 ggaatccttt tgttctcatt gttacatcag ctcttgcctt ggcgataggg atgttgccag
    22741 gttttccgtt ttttgttttt ttcctgatag cagttacttt gacggcttta ttctattata
    22801 aaaaggtcgt agaaaaagaa aaaagtctgt ctgagtctga ttctagtggc tatactggta
    22861 catttgatat tgataatagc catgactcat ctttggcaat gatagaaaat ctggatgcta
    22921 ttagttcaga aaccgttcct ttaattttat tatttgccga aaataagata aatgcaaatg
    22981 atatggaagg tcttattgaa aggataagaa gtcagttctt tattgattat ggtgtcaggc
    23041 ttccaactat tttatataga acaagcaatg agcttaaggt cgatgatatt gttttgttaa
    23101 taaatgaggt gcgtgccgat agttttaata tatattttga caaagtgtgt attacagatg
    23161 aaaatggaga tatagatgct ctaggtattc ctgttgtttc aacttcatat aatgaacgtg
    23221 ttatttcttg ggtagacgtt tcatatacag aaaatctaac taatattgat gctaaaatta
    23281 aaagtgctca agatgaattt tatcaccagt tgtcacaagc tttattaaac aacataaatg
    23341 agatttttgg tatacaagaa acaaaaaata tgttagatca gtttgaaaat cggtatcctg
    23401 atctattaaa ggaagtcttc cgacatgtga ctatacagag aatttctgag gtattacaaa
    23461 gattgcttgg agaaaatatt tctgttcgca atttaaaact tattatggag tctttggcgc
    23521 tttgggctcc cagagaaaaa gatgtcataa cattagttga acatgtccgt gcatcacttt
    23581 ctaggtatat ttgtagtaaa atagctgttt ctggtgagat taaagttgtg atgctttccg
    23641 gatatattga ggatgcaata agaaaaggga taaggcaaac ctctggtggc tctttcttga
    23701 atatggatat agaggtttcg gatgaggtaa tggaaacttt agcacatgct ttgagagaat
    23761 tgagaaatgc aaaaaaaaat ttcgttcttt tggtatcagt agatatacgt aggtttgtta
    23821 aaagacttat agataacaga tttaagagta tactcgttat atcgtatgct gagattgatg
    23881 aagcatatac cattaatgta ttaaagacta tttagtgagg tttaaatatg agtaacatta
    23941 atttagttca attagttaga gatagtcttt tcacgattgg ttgtcctcca tcaattatta
    24001 cagatttaga cagccattca gcgattacca tttctttgga tagtatgcct gctattaata
    24061 tagctcttgt caatgagcag gttatgttat gggctaattt tgatgcacca agtgatgtaa
    24121 aacttcagtc atctgcttat aacatattaa atttaatgtt aatgaacttt agttatagca
    24181 ttaatgagct ggtagagctt catcgttctg atgagtactt acagttaaga gttgttatta
    24241 aagatgacta cgttcacgat gggattgttt ttgcagagat tctgcatgag ttttatcaga
    24301 ggatggaaat cttaaatggg gtcttataat caatgagcta tacaaaattg ctcactcaat
    24361 tatcttttcc taatagaatc tcggggccaa tcttggaaac aagtcttagc gatgtttcga
    24421 ttggtgagat ttgtaacatt caggctggaa ttgaaagtaa tgaaattgtt gcaagagctc
    24481 aggttgtagg atttcatgat gaaaaaacaa tattaagctt gattggaaat tctcgtggac
    24541 tttcacggca aacgttgatt aagcccactg cccagtttct tcatacgcaa gtcggccgtg
    24601 gattattggg agcagtagtc aatcctttag gggaggttac tgataagttt gctgttacag
    24661 ataacagtga aattctttat cgacctgtag ataatgctcc tccgctatat agtgaaaggg
    24721 ctgcaattga gaagcctttt ttaacaggta ttaaggttat tgattcttta ctcacgtgtg
    24781 gtgaaggaca gcgaatgggg atttttgcgt cagctggttg tggcaaaact tttctcatga
    24841 atatgctcat tgaacatagt ggtgctgata tatatgttat tgggttaatt ggtgagcgag
    24901 gtcgagaggt tactgaaacg gttgattatt tgaaaaactc tgagaaaaaa agcaggtgtg
    24961 ttttagtata tgcaacttcg gattactctt cggttgatcg ttgtaatgct gcatatatag
    25021 ccactgctat agccgaattt tttaggactg aaggacataa agtagcgctt tttattgatt
    25081 cattaacaag gtatgccaga gcattacgtg atgtggcctt agccgctgga gaatcacctg
    25141 ccagaagagg ctatccggtt tcggtttttg atagcttacc cagacttctt gaaaggccag
    25201 gaaagttaaa ggcaggtggc tctattactg cattttacac tgttcttttg gaggatgatg
    25261 attttgctga tccattagct gaagaggtaa gatccatttt agatggacat atatatttga
    25321 gcagaaatct agcccaaaaa ggacaatttc ctgcaattga ttccttaaaa agtataagca
    25381 gggtatttac acaggttgtt gatgaaaaac atcgtattat ggccgctgca tttcgggagc
    25441 tactttctga aatagaagag ctaaggacaa ttattgactt tggtgaatac aaaccggggg
    25501 agaatgcctc tcaggataaa atctacaaca aaatatctgt tgttgagagt tttctgaagc
    25561 aagattatcg actcggtttt acttatgagc agacaatgga gcttattggt gaaacaatta
    25621 gataaggttt taaaaatcaa agataaatat caacggtctg ttaaattaat tgaagcacac
    25681 atattaacac taaaaaaaaa actctttgta tcgagatgtg gaggcattag ataaaaggat
    25741 tatatacttc ctgcaactgg agaatgactt agaacctgta ggcgctcaga gtgttagtca
    25801 gcttttcaat acaagacgta agatcgctat agtaaaaaag catattatcc aatatcagtc
    25861 tgaacgaatt cttctaaagg gaagaattga agaaattcaa aaagatattg atgaggcaaa
    25921 tgcttctaaa agaaaactgc ttcataaaga gagtaaaata tgtaaacgga ttggcttgat
    25981 taaaagaaat aactttgcca aacagttgat acttgatgag ttatcacaag aggatatgaa
    26041 gtatggcatt agataatata aacctaaatt ttagtagtga caaacagata gaaaaatgtg
    26101 agaaactatc ttcaatagat aatattgata gtctcgtttt gaagaagaag aggaaggttg
    26161 aaattcctga gtactcttta attgcatcta actattttac tattgataag cactttgaac
    26221 ataagcatga taaaggagaa atttatagtg gcattaaaaa tgcgttcgaa cttagaaacg
    26281 aacgagcgac atattctgat attccagaat caatggccat taaagaaaat attttgatac
    26341 cagatcaaga tatcaaagca agggaaaaaa taaatatcgg cgatatgagg gggatctttt
    26401 catataataa gagtggaaat gcagacaaga acttcgaaag aagtcatact tcttctgtaa
    26461 accctgataa tctgctagaa tctgataata gaaatggtca aattggttta aaaaatcata
    26521 gcttgtctat tgataagaat attgctgaca tcatttcttt actaaatgga agtgttgcta
    26581 aatcatttga gctgcctgta atgaataaaa atactgcaga cataacccca tccatgtcat
    26641 tgcaagaaaa atcaatagtt gaaaatgata aaaatgtttt tcaaaaaaat agtgaaatga
    26701 cttaccactt taaacagtgg ggggctggac attctgttag tatttcagtg gagtctggtt
    26761 cttttgttct aaaaccgtca gatcaatttg taggaaataa acttgactta attttgaaac
    26821 aagatgctga gggtaattac agatttgata gcagtcaaca taataagggg aataaaaata
    26881 atagtacagg atataatgaa cagagtgaag aagaatgcta agaattaaac attttgacgc
    26941 taacgaaaaa ctacagattt tatatgcaaa gcaactctgc gagcgttttt cgattcagac
    27001 attcaaaaat aaatttacag gcagtgaaag tttagtcact cttacttccg tgtgtgggga
    27061 ttgggtaatt cgtattgata cattatcttt tttgaaaaaa aaatacgagg tattttcagg
    27121 attttctaca caagaatctt tactgcattt atcaaaatgt gtctttatag agtcgtcatc
    27181 tgtattttcg attccagaac tgtctgataa gattactttc cggatcacga atgaaatcca
    27241 gtatgcaact actgggagtc atttatgctg tttttcctct tctttaggta ttatttattt
    27301 tgacaagatg ccggtattac gtaatcaagt ttctcttgac tcattgcatc atcttttaga
    27361 gttttgctta ggttcatcta atgtaaggct ggctacttta aaaagaattc gcactggtga
    27421 tataatcata gttcagaaac tttataattt attattgtgt aatcaagtta ttattgggga
    27481 ttatattgtg aatgataata atgaggcaaa aattaatctg tcagaaagta atggtgagtc
    27541 agaacacaca gaagtttctt tggcattatt caattatgat gatatcaatg taaaagtgga
    27601 ctttattctt ttagaaaaaa atatgacaat caatgaacta aaaatgtatg tagaaaacga
    27661 attattcaag tttcccgatg acatagttaa acatgtaaat attaaagtaa atggttcttt
    27721 ggttgggcat ggggaacttg tttctattga ggatggttat ggtatcgaga ttagttcttg
    27781 gatggtaaag gagtaatctc atgctgagtg acatgtccct catcgctaca ctttcttttt
    27841 ttacgctatt accattttta gttgctgctg gtacctgtta tataaagttt tctattgttt
    27901 ttgtaatggt acgaaatgca cttggtttgc agcaggttcc atcaaatatg actctcaatg
    27961 gtattgcatt gattatggct ttgttcgtga tgaaacccat tattgaagct ggttatgaaa
    28021 attatctaaa tggccctcaa aaatttgata caatatctga tattgtccgt ttttctgatt
    28081 ctggcttgat ggaatataag caatacttga aaaagcatac agatcttgag ttagcacgtt
    28141 ttttccaaag gtctgaagaa gaaaatgctg atttaaagag tgcagagaat aatgattatt
    28201 cattgttttc cctcttgcct gcatatgcgc taagtgaaat caaagatgcg ttcaaaatag
    28261 gtttttattt atatttgcca tttgttgttg ttgatcttgt tatttcaagc attttacttg
    28321 cacttggtat gatgatgatg agtccaatca caatatcagt accaataaaa cttgtattat
    28381 ttgttgcgct tgatgggtgg ggtatcctgt ctaaggcctt gattgaacaa tatatcaata
    28441 ttcctgctta gatgatggag gtaatgtgtc tgatatagtt tatatgggta ataaggctct
    28501 ttatttaatc cttatctttt ccttatggcc agtaggtata gctacggtta ttggattatc
    28561 tattggtttg ttacagacag tgactcaact ccaagagcag acacttcctt ttggtataaa
    28621 gcttataggt gtctcaatat ctttgctact tctttctgga tggtatggtg aggttttatt
    28681 gtctttttgt catgaaataa tgtttttaat taagagtggg gtttgatgga catttcaagc
    28741 tggttcgaaa gtattcatgt gtttttaata ctcctgaacg gcgttttttt tagattggct
    28801 ccattatttt tctttcttcc atttttaaat aacggtataa tttctccatc tattagaata
    28861 cctgtgattt ttcttgttgc atcaggatta attacttctg gtaaggtaga cataggttct
    28921 tctgtttttg aacatgttta tttccttatg ttcaaggaaa taattgttgg cctccttctc
    28981 tctttttgct tgtctcttcc cttttggata tttcatgctg ttggtagcat tattgacaac
    29041 cagcgtgggg caacgcttag tagttcaatt gatcctgcca atggtgttga tacgtctgag
    29101 ttggcaaaat ttttcaatct tttttctgca gttgtatttc tatacagtgg tggtatggtc
    29161 tttattttag aatccataca attgtcttat aatatatgcc cgttattttc tcaatgttct
    29221 ttccgtgtct caaatatctt aacatttctg actttattgg caagtcaggc tgttatttta
    29281 gccagtcctg ttatgatagt attgttacta tcagaagtat tacttggtgt attatcgaga
    29341 tttgctccgc agatgaatgc tttttccgta tcattaacta ttaaaagttt acttgcaata
    29401 tttattattt tcatctgttc ttctactatt tacttttcta aagttcaatt tttcctcggt
    29461 gaacataaat ttttcacaaa tctatttgtt agataaaata ttatggcaaa taaaacagaa
    29521 aagccgacac ctaaaaaact aaaggatgcc gcaaaaaaag gacagtcatt taaatttaag
    29581 gatttaacga ctgttgttat tattctggta gggacattta ctataatatc attcttttcc
    29641 ttaagtgatg taatgctctt atacagatat gtaataatta atgacttcga aattaatgag
    29701 ggtaaatact tttttgcagt ggttattgtc ttttttaaga taattggctt cccacttttt
    29761 ttctgtgttc tttcggctgt gttgccaaca ttggttcaaa caaagtttgt tcttgcgact
    29821 aaagctatca agattgattt ttcagtatta aaccctgtta aagggttaaa aaaaatattt
    29881 agtataaaga caataaaaga atttttcaaa agcattctgc ttcttattat tctagcatta
    29941 acaacctatt tcttttggat taatgaccga aaaataattt tttctcaggt gttttctagt
    30001 gttgatggct tatatcttat ttgggggagg ctgtttaagg atataatatt atttttcttg
    30061 gcattttcta ttcttgttat tattcttgac tttgtgattg agttcatttt atacatgaaa
    30121 gatatgatga tggataaaca ggagataaaa agagaatata tagagcaaga gggacacttt
    30181 gagacaaagt cgagaaggcg tgagttgcat atcgagattc tttcagagca gactaaatct
    30241 gatatacgta attcaaaatt agtggtaatg aacccgactc atattgcaat tggtatttat
    30301 tttaatccag aaatagcgcc tgcacctttt atttctctca ttgaaactaa ccagtgtgcc
    30361 ttggctgtca gaaaatatgc aaatgaagtt ggtataccga ctgtgcgtga tgtgaaatta
    30421 gctagaaaac tatataaaac acatacaaaa tatagttttg ttgattttga acacttggat
    30481 gaagtcctac gtcttattgt ttggcttgag caggttgaaa acactcatta ataagtaaag
    30541 gagatgtgtt atatgggagt taatttctgt aataaaatag gtattgatca gtctgaattt
    30601 gaaatagaat cttccatcat taactccatt gctaatgagg tattgaaccc aatatctttc
    30661 cttagcaata aggatataat aaatgtttta ctcagaaaga tttcatctga atgtgacctg
    30721 gttagaaaag acatttatcg ttgtgctctg gagttggtcg ttgaaaaaac acctgatgat
    30781 ttataatcat tcataataac tcttttgctg atataaatat ttttgttgaa gatatttatg
    30841 tagtcacagg atcaattgta gaaaattgtt atgtttttgt taggaactgc actaaataat
    30901 atatgataag acagcaaaaa cgactgacca taatccttct tttattagga gttgataaga
    30961 gagactattc atcctgtaat gttaaaactc tgttatatag tattcgcgat tatgcaaagt
    31021 ctgtaaatga tcatgagata ctaaccgaga gtaatcgttt attatctcat tgcatttcag
    31081 atagtaacgg agctttcttt aaaagtagta aatacgttcc ccttaagtac ttacgtaaaa
    31141 ggagaatagc acgaaagata ccaaatgatt agcactttgc tagtaagctt gctggtaaac
    31201 tatattgacc gttgggtttg ttttgagact gtaatttaaa ttgtgtaatt gcctgttttt
    31261 gatatgttta ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc
    31321 ttgcggctga actggctaaa ggcctcaaaa ctgaggccga cctcaatcag ttttcccgta
    31381 tgctgacgaa atatcccggc tcttaaaacg atagtggctc aggctttcat ttttcagcgt
    31441 tccccagaag
//
LOCUS       DAJVPT010000040        30012 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVPT010000040 DAJVPT010000000
VERSION     DAJVPT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQM47_001804"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQM47_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1479989.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQM47_001805"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQM47_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1479990.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OQM47_001806"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OQM47_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1479991.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OQM47_001807"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OQM47_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1479992.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OQM47_001808"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OQM47_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1479993.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OQM47_001809"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OQM47_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1479994.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OQM47_001810"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OQM47_001810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1479995.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OQM47_001811"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OQM47_001811"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1479996.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQM47_001812"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQM47_001812"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1479997.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQM47_001813"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQM47_001813"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1479998.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQM47_001814"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQM47_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1479999.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQM47_001815"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQM47_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1480000.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQM47_001816"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQM47_001816"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1480001.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQM47_001817"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQM47_001817"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1480002.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQM47_001818"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQM47_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1480003.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OQM47_001819"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OQM47_001819"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1480004.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OQM47_001820"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OQM47_001820"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1480005.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OQM47_001821"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OQM47_001821"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1480006.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OQM47_001822"
     CDS             21622..22212
                     /locus_tag="OQM47_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1480007.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OQM47_001823"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OQM47_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1480008.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OQM47_001824"
     CDS             22684..23379
                     /locus_tag="OQM47_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1480009.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQM47_001825"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQM47_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1480010.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OQM47_001826"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OQM47_001826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1480011.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OQM47_001827"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OQM47_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1480012.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQM47_001828"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQM47_001828"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1480013.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQM47_001829"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQM47_001829"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1480014.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7824 c         8255 g         6905 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJVPT010000041        29828 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVPT010000041 DAJVPT010000000
VERSION     DAJVPT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1105
                     /locus_tag="OQM47_001830"
     CDS             <1..1105
                     /locus_tag="OQM47_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1480015.1"
                     /translation="LDFLARELSSHPQNLSDGQIRQGLSAMVQLLEHYFSEQGAGQAR
                     YRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQAKGTLRLDKDAR
                     PLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVEHVCRLVRESSR
                     VAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWVINELARQCGHH
                     FDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATVRSLLNNDREAE
                     REHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAIHRSSKPDLAIE
                     VAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1102..2058)
                     /locus_tag="OQM47_001831"
     CDS             complement(1102..2058)
                     /locus_tag="OQM47_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1480016.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2209..3324
                     /gene="macA"
                     /locus_tag="OQM47_001832"
     CDS             2209..3324
                     /gene="macA"
                     /locus_tag="OQM47_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1480017.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3321..5267
                     /gene="macB"
                     /locus_tag="OQM47_001833"
     CDS             3321..5267
                     /gene="macB"
                     /locus_tag="OQM47_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1480018.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5340..5564)
                     /gene="cspD"
                     /locus_tag="OQM47_001834"
     CDS             complement(5340..5564)
                     /gene="cspD"
                     /locus_tag="OQM47_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1480019.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            5887..6207
                     /gene="clpS"
                     /locus_tag="OQM47_001835"
     CDS             5887..6207
                     /gene="clpS"
                     /locus_tag="OQM47_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1480020.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6238..8514
                     /gene="clpA"
                     /locus_tag="OQM47_001836"
     CDS             6238..8514
                     /gene="clpA"
                     /locus_tag="OQM47_001836"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1480021.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(8917..9004)
                     /locus_tag="OQM47_001837"
     tRNA            complement(8917..9004)
                     /locus_tag="OQM47_001837"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8968..8970),aa:Ser,seq:gga)
     gene            complement(9258..9476)
                     /gene="infA"
                     /locus_tag="OQM47_001838"
     CDS             complement(9258..9476)
                     /gene="infA"
                     /locus_tag="OQM47_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1480022.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(9761..10465)
                     /gene="aat"
                     /locus_tag="OQM47_001839"
     CDS             complement(9761..10465)
                     /gene="aat"
                     /locus_tag="OQM47_001839"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1480023.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(10507..12228)
                     /gene="cydC"
                     /locus_tag="OQM47_001840"
     CDS             complement(10507..12228)
                     /gene="cydC"
                     /locus_tag="OQM47_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1480024.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12229..13995)
                     /gene="cydD"
                     /locus_tag="OQM47_001841"
     CDS             complement(12229..13995)
                     /gene="cydD"
                     /locus_tag="OQM47_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1480025.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14118..15083)
                     /gene="trxB"
                     /locus_tag="OQM47_001842"
     CDS             complement(14118..15083)
                     /gene="trxB"
                     /locus_tag="OQM47_001842"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1480026.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            15627..16121
                     /gene="lrp"
                     /locus_tag="OQM47_001843"
     CDS             15627..16121
                     /gene="lrp"
                     /locus_tag="OQM47_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1480027.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16256..20284
                     /gene="ftsK"
                     /locus_tag="OQM47_001844"
     CDS             16256..20284
                     /gene="ftsK"
                     /locus_tag="OQM47_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1480028.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            20439..21050
                     /gene="lolA"
                     /locus_tag="OQM47_001845"
     CDS             20439..21050
                     /gene="lolA"
                     /locus_tag="OQM47_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1480029.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21061..22404
                     /gene="rarA"
                     /locus_tag="OQM47_001846"
                     /gene_synonym="mgsA"
     CDS             21061..22404
                     /gene="rarA"
                     /locus_tag="OQM47_001846"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1480030.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22495..23787
                     /gene="serS"
                     /locus_tag="OQM47_001847"
     CDS             22495..23787
                     /gene="serS"
                     /locus_tag="OQM47_001847"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1480031.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24027..26470
                     /gene="dmsA"
                     /locus_tag="OQM47_001848"
                     /pseudo
     CDS             24027..26470
                     /gene="dmsA"
                     /locus_tag="OQM47_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            26481..27098
                     /gene="dmsB"
                     /locus_tag="OQM47_001849"
     CDS             26481..27098
                     /gene="dmsB"
                     /locus_tag="OQM47_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1480032.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27100..27891
                     /locus_tag="OQM47_001850"
     CDS             27100..27891
                     /locus_tag="OQM47_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1480033.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(27927..28553)
                     /locus_tag="OQM47_001851"
     CDS             complement(27927..28553)
                     /locus_tag="OQM47_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1480034.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28868..>29828
                     /locus_tag="OQM47_001852"
     CDS             28868..>29828
                     /locus_tag="OQM47_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480035.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLV"
BASE COUNT         7361 a         7352 c         8051 g         7064 t
ORIGIN      
        1 gctcgatttc ctcgcccgtg agttgtcctc acatccgcaa aatctctctg atggacagat
       61 tcgtcaggga ctttccgcaa tggtgcagtt gcttgagcat tatttctctg agcagggggc
      121 tggacaggcg cgatatcgtt taatgcggcg gcgggccagc aatgagcaac gaagctggcg
      181 ctatctggac atcatcaacc ggatgattga ccgacctgga gggcgctcgt atcgggttat
      241 tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc acattgcgac tggataaaga
      301 cgcgcgtccg ttattgctaa ttgaagatcc agaaacccgc ctgcatccga ttatgctttc
      361 cgttgcctgg catctgttga atcttttgcc tttacagcgt attgctacca ccaactctgg
      421 cgagctgctt tcgttaactc cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg
      481 tgttgccgcc tggcgtctgg ggccaagtgg cttgagcacc gaagacagcc gccgcatctc
      541 ttttcacatt cgttttaatc gtccgtcatc gctgtttgct cgctgctggt tgctggtgga
      601 aggggaaacg gaaacctggg ttatcaatga actggcgcgt cagtgcggac atcattttga
      661 tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt
      721 tgcccgccga atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa
      781 atatgccgct acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt
      841 aacggcgtta ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt
      901 gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg aatctacgca aaattatctc
      961 gaaagcgatc catcgctctt ccaaacccga tcttgccatt gaagtggcaa tggaggcagg
     1021 acgtcgtggt gtggactccg taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg
     1081 gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc
     1141 gtcgagcatc tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat
     1201 ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc
     1261 aaccgactcc cagaacgcgt tgtaatcagc atggatcttg ccttctttat cgcgataacg
     1321 caggctgcgg taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg
     1381 ttgggcaaac agacaggccg cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc
     1441 tttcgtcgca ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc
     1501 aataaacatc gttcttttcc cctgatattc acacagggta aacgtgatct ctgccagagg
     1561 aataccttcg ctgttgcgga acaggattgt gctgtcacct tctttatcca ttgagatcat
     1621 catggtcagc tcaagcgtga actgctcacc gtttttgcct tccagtttag ccagttgcag
     1681 cccgggggta ttcaaatata agctgaattc ttccgccgac atacacccgc ggagtaacgc
     1741 ataatggtaa cgtaacgcct ccagcaattg cttacggcta agattcgcgg caaggtaagg
     1801 gcgatgcaga cgcacaggca gtcgcggctg gcgcgttaac aacacattga gattaggcca
     1861 gtgggaaagt tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa
     1921 aaatttctgg cgaaaactac agcgatgcca gaatttaccc ggccgacact gtccacgtgc
     1981 cagactaaga aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt
     2041 tagctgcgac atattcatga aatcaatggt tatacatgac gtcgatttca ccattgcgta
     2101 tcttaaccaa acatcaatag tgtgattact aacgtaaatt ttagggtttt gttgatattt
     2161 cgttgaagtt aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg
     2221 aaaaccgtga agaagcgtta cgttattgcg ctggtgatag tcatcgccgg actgattacg
     2281 ttatggagaa ttcttaacgc acccgtgccg acttatcaga cactgattgt gcgccccggt
     2341 gatttacagc aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg
     2401 ggcgcgcagg tcagcggtca gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa
     2461 aaagaccagc ttttaggggt cattgatcct gaacaggctg aaaaccagat caaggaggtc
     2521 gaagcaacgc tgatggagct acgcgcgcag cggcagcagg cggaagcgga gctgaaactg
     2581 gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag
     2641 gatctcgata ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac
     2701 gcgcaaatca agcgcaatca ggcctctctc gatacggcta aaaccaatct cgattacacc
     2761 cgtatcgttg ccccgatggc tggggaagtg acgcaaatca ccactctgca aggccagacg
     2821 gtgattgccg cacagcaagc accgaacatt ctgacgctgg cagatatgag cactatgctg
     2881 gtaaaagcgc aggtttctga agcggatgta atccacctga agccggggca aaaagcctgg
     2941 tttacggtgc ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtgctaccg
     3001 acgccggaaa aggtaaacga cgctattttc tattacgccc gttttgaagt acccaacccc
     3061 aatggtttgc tgcggctgga tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa
     3121 aatgtgttga cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa
     3181 gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac
     3241 gataccgatg ttgagattgt caaagggctg gaagcgggcg atgaagtggt gattggtgag
     3301 gccaaaccag gagctgcaca atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct
     3361 atcctgccgg tgatgagcag gttgaggtgc tgaagggcat cagcctcaat atttatgcgg
     3421 gtgagatggt cgcgattgtt ggcgcttcgg gttccggtaa atcgaccctg atgaatattc
     3481 tcggctgcct ggataaggcc accagcggca cctatcgcgt agccggtcag gatattgcca
     3541 cgctggacgc cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt attttccagc
     3601 gttaccattt gcttccgcat ttaaccgtcg agcagaacgt tgaagtaccc gccgtctatg
     3661 ccggtcttga gcggaaacag cgactgcttc gtgcccagga gttgctgcaa cggctggggc
     3721 tggaagaccg tacagagtat tatccggcac agctttcggg tggtcagcaa cagcgcgtca
     3781 gcatcgcgcg ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccgaccggcg
     3841 cactggacag ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg
     3901 ggcatacggt gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga
     3961 tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg
     4021 ctggcgggac ggagcctgtt gtcaatacag tgtctggctg gcggcagttt gtcagcggtt
     4081 ttaacgaggc gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac
     4141 tgaccatgct ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg
     4201 acgccgccaa acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg
     4261 tctatcccgg gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg
     4321 acgacttaat cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc
     4381 aaaacctgcg cctgcgttat aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg
     4441 gcgattattt taatgtctat ggcatgacct tcagtgaagg aaacaccttt aatcaggagc
     4501 agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc
     4561 cccataaagc agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca
     4621 ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc
     4681 taccttacag cacgatgtcc gggcgagtta tgggccagtc gtggcttaac tccattactg
     4741 tcagggtgaa agaaggattt gacagcgccg aggcggaaca gcaactcacg cgtttacttt
     4801 cactgcgcca cggtaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaactg
     4861 ttgaaaagac cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg
     4921 tggtgggcgg tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg
     4981 aaattggcat tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga
     5041 tcgaagccgt actggtttgc ctggtcggtg gcgcgttggg aataacactg tcactgttaa
     5101 ttgctttcac cttgcagctt ttcttacccg gctgggagat tggtttttca ccgttggcgc
     5161 tgctgctggc gtttctctgc tcgacggcca ccgggatttt atttggctgg ttacccgcac
     5221 gaaatgcggc acgactggat ccagtagatg ctctggcacg ggagtaattt ttgagataaa
     5281 aatgccagcc gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc
     5341 tatgcgactg ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc
     5401 ccctggtgga catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc
     5461 tgaatggtgg aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca
     5521 aaccctttgg cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg
     5581 caaatcttat acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct
     5641 cgccaactct agaaatacaa tttagagaat tagggcgagc cgtcaagcat ttgacagggg
     5701 ccagggggca ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac
     5761 agatgtcgct gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag
     5821 gcaaataagc tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa
     5881 ctgacaatgg gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt
     5941 cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact
     6001 ccgatggagt ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca
     6061 acgcaattga tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc
     6121 gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca
     6181 ttgctgtgta cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg
     6241 ctcaatcaag aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt
     6301 catgagttta tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg
     6361 gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt
     6421 attgaacaaa ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg
     6481 ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat
     6541 gaggttaccg gtgcaaacgt tctggtcgct atcttcagcg aacaggagtc gcaggcggca
     6601 tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc
     6661 acgcgtaaag acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa
     6721 caagctggtg gggaggaacg tatggagaat ttcacgacga acctgaacca gcttgcgcgt
     6781 gtgggcggaa tcgacccact aattggtcgt gagaaagagc tggagcgtgc tattcaggtt
     6841 ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc
     6901 gcgattgcag aaggtcttgc ctggcgaatt gttcagggtg atgtgccgga agtgatggct
     6961 gactgtacga tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc
     7021 gactttgaaa aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc
     7081 ctgtttattg atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc
     7141 gatgcggcta acctaatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg
     7201 acaacctatc aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc
     7261 cagaaaattg atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg
     7321 aaaccgaagt atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg
     7381 gtagagctgg cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgatgtt
     7441 atcgacgaag caggcgctcg cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt
     7501 aatgtggcgg atattgagtc cgtggtggcc cgtattgcac gcattccaga gaagagtgtt
     7561 tctcagagtg accgcgatac cctgaaaaaa ctcagcgatc gcctgaaaat gctggtcttc
     7621 ggccaggata aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta
     7681 ggtcacgaac ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa
     7741 acagaggtga cggtacagct ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg
     7801 tccgagtata tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt
     7861 ggttttgatc agggcggttt gctgaccgat gcggtaatca agcatccaca tgcggtgctg
     7921 ctgctggacg aaatcgagaa agcgcatccg gacgtgttca atattctgtt gcaggtgatg
     7981 gacaacggta cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg
     8041 gtgatgacca ctaacgccgg ggtacgggaa actgagcgca aatccattgg tcttatccac
     8101 caggataaca gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt
     8161 aaccgtctcg acaacattat ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg
     8221 gtggataaat tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa
     8281 gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca
     8341 cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg
     8401 tttggttcgc tggtggacgg cggtcaggtg acggttgcgc tggataaaga gaaaaatgag
     8461 ctgacttacg gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat
     8521 tgtcaggtag gttggtgaag tccgtaatct cgtcaggggt tacggacttt ttatttatgg
     8581 ggtggaacgg tgaaaaccct atttttggag gtgaaggtaa gttgttgata attagtgctg
     8641 ctggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg ctttattaac
     8701 aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag aaatcgacgg
     8761 ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca ggcagcttag aaattcacag
     8821 gtaacatact ccacccgccc accatgttca ctgccgtaca gacagataaa atgcgaaaaa
     8881 aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac
     8941 ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct
     9001 caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact atagggagtt
     9061 ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata ctgtaaacaa
     9121 cttgtgcagt atatctacat cgagacaagt tatggactta tacttccaaa gtacttcata
     9181 catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt ctcttcgccc
     9241 atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct caggtcgtac
     9301 ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt
     9361 ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg gaacatggta
     9421 ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt ggccatctaa
     9481 tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat
     9541 aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact ccgaaaacac
     9601 acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa cgatgggcgt
     9661 tacctgacgc gaaaaattcc ttatcggcag cggggtaatg agcgtaacca actctgcgac
     9721 cgcaattata acactctggg gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca
     9781 agcaccgtgg tacccagaaa ttattcggca atcgtccgag gcgcatttga ttgagataat
     9841 taaggtaatc ccggcgggga atttcgcagg caccaagcga tgctgtgtga tcgttaagga
     9901 cctggcagtc gataagctta ccgccatgac cgataaattc ctcacagaat accagaagcg
     9961 ccgttttaga cgcattttcc atccggctga acatggactc gccacaaaat agcgttccct
    10021 gggccacgcc gtacataccg ccgacaagct catcttcacg ccagacttca atggagtggg
    10081 catgaccgag ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg atccaggttc
    10141 cttcttcgcg atcgctggca cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga
    10201 cacgataggg cgagcgttta tgaaatcgct tcatactacg gctgatatgc agtgattctg
    10261 gccatagcac cgcgcgggga tcgggcgacc accagaggat ggggtcgtct ggagaaaacc
    10321 acggaaaaat accacgctgg taagccatta acaggcgcgc agggctaaga tcgcccccaa
    10381 gtgccagcag gccgttaggc tcacgtaatg cgccttccgg ggaagggaag gctattgaat
    10441 ggcgagaaag ctgaaccaag cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat
    10501 aatagcttac aaaccctgct tgaactggta ataacgcccc tgtctggcaa gcagttctgc
    10561 gtgagtacct tgctcaataa tttgcccgtt gtccatcact attatttgtt ggaaacgaga
    10621 gagtccgcga agtcgatggg tgaccattaa caccgttttc tcacgcatca tttttgcaag
    10681 caattcaagg atctggcttt cggttgtggc atctaagcct tcggtaggtt catccagcaa
    10741 caccagtggc gcatcatgta acagcgtacg ggcgatagcc agacggcgca gttcaccacc
    10801 ggagagctgg cgtccgcctt cacctaacca actgttgaga cctgcatcct cgagcagctt
    10861 ctccagacca acgcgacgca agatctccga cagagcctca tcactactgc caggcgaggc
    10921 gagtaacaga ttatcacgca gtgtggcgct aaacagatgc actcgctgag gaacaacgct
    10981 gatggtctgt cgtagagccg cttcattcag gctggctatg gggctatcgt taagcaaaat
    11041 ctcgccctgt tgcgggtccc atgcgcgggt cagcagttgt aacagtgttg atttgccgca
    11101 tccggttcgc ccgagaatcg ctatatgttc cccggcgttt acctgaagag aaatcccttt
    11161 aagtgcctgt tgggattgct ccggataagt gaactgaaca tcccgtaatg tcagcgaaac
    11221 gcgatcggca acacgagttt gggtatcagg gaaggtgacc tccggttttt gatccgttaa
    11281 gtcagagata cgtacggcag aggcaatgac ttgccccaga tgctgaaatg cacccgttac
    11341 tggtgccagt gcttcaaacg cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc
    11401 gggttgagca ttgccgccaa cgccgccaga cgccatccac agcatcagga tcaccgctaa
    11461 cgcgccaatg agcagcatta tcgcttgcga caatgcggtc agttcagatt gacggcgttg
    11521 cgcttccagc cattgaattt ctgtattctc tagttgcgtg cgataacgat cgctggcacc
    11581 aaaaatggtc agctcagctt gcccttgcag ccaggccgtc agttgttggc gatactgtcc
    11641 gcgaagatga gtcagatttt gcccggtgct ttttcccgca cgataaaaca gcggtggcat
    11701 caggaaaagc gtcagtaaca taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa
    11761 acttaacccg attgtcacca ccataatcac cacaaaagcg cccaccagcg gcgagataac
    11821 gcgcaggtaa agatgatcga gcgtatcaac atccgccacc acgcggttga gcaattcgcc
    11881 ctgacgatag cgcgccagtc cggcagggga gaggggcaac aatttgctga aggtgtaaat
    11941 gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt caaaataacg
    12001 cccggcagta cgggtgattg cggcaccacg cacgcccgca gcgggtagca tatagttgaa
    12061 gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt
    12121 caacagaccg atactggcga gcagcgtcac aattgccagc acaataccaa gacttaacat
    12181 ccatttatga cgtttataca gtgccagata gggtagcaaa gcgcgcattt aaatctcctc
    12241 ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg
    12301 tccttgctca ataatccggc catcctgcat aacccaaatg acatcccagt cagcaagatc
    12361 ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc
    12421 cattacgcgc tgttcactgt gagcatcaag gctggcagcg ggttcatcca acagtaatag
    12481 cgaacaggga tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag
    12541 gcgggcagcc tggtcgccaa caggtgtatc aacgccttgt ggcaggagcg gtagaaactc
    12601 gctgacccag gcgttatcca gcgctgcttg taattcttgt tcgctggcat caggtcgcgc
    12661 cagtagtacg ttatcccgca attttgctgc cggtaattgt gggttttgcc caacccagga
    12721 gagatgttta cgccatgatt ctggtgataa atcacgtaat tctatcccgt tgattcgtaa
    12781 tgatccctga tatgagagaa aaccagaaag cgcgttcagc agtgagcttt tacctgaacc
    12841 gctgcggcca accaacactg cacgttggcc tgctggcaaa gtaaagttca gcggtccggc
    12901 cagcgtttta ccttccggcg acgtgataaa cagatcctcg gcctcaatgg tcagcggatc
    12961 ggtcaatgct aattccgcct caccgcgctg cggatgggcg agcggggttt ccataaacgt
    13021 tttcagactg tcagctgcac caacagcctg ggctttagca tgataaaacg taccgagatc
    13081 gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc agaaaacctg cagccagcgt
    13141 caccccagta tcgtagtgac caaaatccag ctcgccgaga taggaaaaac caaagtagac
    13201 cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc
    13261 cagtcgtagc acttccattg tccgttggcg gaaatcttcc gaagcagaac gaatactttc
    13321 aatttcagct tcaccacgac caaaaatacg caatgtttcc atgccgcgca ggcgatcgag
    13381 gaaatgccca cttaagcgag caagagcgag aaagttacgc cggttagcat cggcagcccc
    13441 cattccaacc agcgccataa acaacggaat tagcggtgct gtgcccagca gaatgagcgc
    13501 cgcagcccag ttagagggga agatcgccac cacaatcagc aacggcaccg acactgccag
    13561 cgccatttgc ggcagatagc gtgcatagta atcatgcata tcgtcaattt gctcgagtac
    13621 cagcgtcgcc cagctccccg caggtttacc ctgaatccac gctggccctg cttgttgcag
    13681 acggtcgaga acctgacggc ggatggcaaa gcggatatgc tgcccggcgt gataacccac
    13741 ccgttcgcgt aaccagacca cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa
    13801 gggaagcagc agggcttcac ggggaatatt ctccataatc atatgttgca gaattcgcgc
    13861 catgaaccag gcctgggcaa tgatcaatat gccgcttaca aagcccagca gacgagaaat
    13921 attcagccaa cgttgggaga tgacgctttg ctgttttaac cagcgggtta actctttttg
    13981 acgagattta ttcattgcac gcttagcagg tgagttatca gaattatttg cagagcaatg
    14041 ttacaacggg gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt
    14101 gttactgatt tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca
    14161 agtgctgcca tgcagccagt accggccgaa gtaatggcct ggcgataaat gtgatccatc
    14221 acgtcgcctg cggcaaagac gccaggaatg ctggtctggg tggcattacc atgaataccc
    14281 gactgtactt tgatgtagcc gttttccagt tccagctgcc cttcgaaaat cgcagtattc
    14341 gggctgtgac cgatagcaac aaacagaccg gcaacgtcga gtgactcgat gttatcgctg
    14401 ttttgcgtat cgcgcagacg aacgccagtg acacccattt gatcgccggt cacttcttcc
    14461 agcgtacggt tggtgtgcag aatgatgttg ccgttctcca ctttatccat cagacgctta
    14521 ataaggattt tttccgcgcg gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg
    14581 atgttagaca gatacagcgc ctcttcaacc gcggtattgc cgccgccgat gaccgcaact
    14641 ttctggttgc gatagaagaa accgtcgcag gttgcacaag cagaaacccc acggccttta
    14701 aaggcttctt cagagggcag gccgagatag cgtgcagaag ctccggtggc aataatcagc
    14761 gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga acggacggtt ttgcagatcc
    14821 accttgttga tatgatcaaa aatgatctca gtttcaaact tggtggcatg ttcgtgcatg
    14881 cgcgccatta ataacggacc ggtcagatcg tttggatcac caggccagtt ttccacttcc
    14941 gtggtggtgg tcagttggcc gcctttttcc atgccggtaa tcagcacagg ttgcaggttg
    15001 gcgcgcgccg cgtagacagc agcggtgtat cccgccgggc ctgaacccag gataagcagt
    15061 ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt tggcagacaa tgggcaggat
    15121 tgtagggaat ttacagacgt aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat
    15181 cggcagcatc gataagcagg tcaaattctc ccgtcattat cacctctgct acttaaattt
    15241 cccgctttat aagccgatta aatgatgaat aaacgcccct gttaatgaat atctggcatg
    15301 ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg tgttttgcga aaacattcga
    15361 ggaagaaaaa aacagtattc ttatatgcgc ataaccatgt atgtaaatac catgtttacc
    15421 gtgctagtga aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca
    15481 caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt
    15541 cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag
    15601 ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc
    15661 tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg
    15721 tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc
    15781 tggaaagaca agggtttatt cagggctata cggcgctgct taacccccat tatctggatg
    15841 catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac
    15901 aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatctggtg
    15961 atttcgacta cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg
    16021 gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag
    16081 aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat
    16141 cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt
    16201 actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag
    16261 ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgccgcct
    16321 tctggaggca ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact
    16381 aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa
    16441 tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt ttggcgtgat
    16501 ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc
    16561 cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct
    16621 catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc
    16681 cggtggcgtc attggcagct tactaagcac tacgctacaa ccactgctac acagtagcgg
    16741 gggaactatt gcgctgctct gcgtttgggc agcgggcctg acgttgttca ccggttggtc
    16801 atgggtgacc attgctgaaa aactcggcgg ctggatttta aacattctca ccttcgccag
    16861 taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gagtatgaag acgacgaaga
    16921 gtatgaagat gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg
    16981 cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac
    17041 agacgctgcg ttgttctccg gtaagcggat ggatgatgac gaagagatta cctacactgc
    17101 acgcggtgtg gctgccgacc cggacgacgt cctattttcg ggcaatcgtg caacgcagcc
    17161 agaatatgac gaatacgatc cattattaaa cagtgcgcca attaccgaac ctgtcgctgt
    17221 agcagctgct gctaccacgg cgacacaaag ctgggctgcg ccggttgaac ctgtgactca
    17281 gacgccgcct gttgcctctg ttgatgttcc accttcgcaa cctacagtag cctggcagcc
    17341 tgtaccgggt ccacaaacgg gagagccggt tattgctcct gcaccggaag gttacccaca
    17401 gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aaccagtaca
    17461 gccgcagcag ccgtattatg cacctgcagc tgaacaacct gcgcaacagc cgtattatgc
    17521 ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc agcaatccac
    17581 ttttgctcca cagtctacat accagactga gcaaacttat cagcagccag ccgctcagga
    17641 gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc ctgaacccgt
    17701 tgtagaagag acaaaacccg cgcgtccgcc gctttactat tttgaagaag tggaagagaa
    17761 gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag aaccggttaa
    17821 agaaccagaa ccgatcaaat cttcgctgaa agcaccttct gttgcagcag tacctccagt
    17881 agaagccgct gccgctgttt ctccgctggc atctggcgtg aaaaaagcga cactggcgac
    17941 gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg gtggaccgcg
    18001 tccgcaggtc aaagagggga ttggcccgca gttgccacga ccgaaacgta tccgcgtgcc
    18061 aactcgtcgt gaattggcgt cttacggtat taagctgccc tcacagcgtg cggcggaaga
    18121 aaaagcccgt gaagcccagc gcaatcagta tgattctggc gatcagtata acgatgatga
    18181 aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacac agcagcaacg
    18241 ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag atgctgcggc
    18301 agaggctgaa ctggctcgtc agtttgcgca aactcaacaa caacgttatt ccggcgaaca
    18361 accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt
    18421 gctggatgat ggtccacacg aaccgctgtt tacgccaatt gttgaacctg tacagcagcc
    18481 gcaacaaccg gttgcaccac agcagcagta tcagcagcca caacagccgg ttgcgccaca
    18541 gcagcagtat cagcagccac aacagcaggt tgcgccgcag ccacaatatc agcagccgca
    18601 acaaccggtt gcgccacagc agcaatatca gcagccgcaa caaccagttg cgccgcagcc
    18661 gcagtatcaa cagccgcaac aaccggttgc accgcagccg cagtatcagc agccacaaca
    18721 gccagttgcg ccacagccgc aggataccct gcttcatccg ctgttgatgc gtaatggcga
    18781 cagccgtccg ttgcacaaac cgacgacgcc actgccttct ctggatttgc tgacaccgcc
    18841 gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa caaatggcgc gtctggtgga
    18901 agcgcgtctg gctgatttcc gtattaaagc cgatgtcgtt aattactctc cggggccagt
    18961 tatcactcgc tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt
    19021 gtcacgggac cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc
    19081 tggcaaaccc tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg
    19141 cgaagttttg gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg
    19201 taaagatatc gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt
    19261 tgcggggact accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct
    19321 ttataaagca cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct
    19381 ttcggtttat gaaggcattc cgcacctgtt aacggaagtc gttactgata tgaaagatgc
    19441 cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt cggtataaac tgatgtctgc
    19501 gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat
    19561 gcgtccgatt ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt
    19621 gctgaaaaaa gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc tgatgatgac
    19681 ggtaggtaaa aaagtggaag agctgatagc tcgtctggcg caaaaagccc gtgccgcggg
    19741 tatccacctc gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa
    19801 agcgaatatt ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat
    19861 tcttgatcag gctggcgcgg aatcactgct gggtatgggg gatatgctct actctgggcc
    19921 gaactccacg ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc
    19981 cgtggtgcag gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacctccga
    20041 cagcgaaagc gaaggtggtg cgggtggttt cgatggcgct gaagaactgg atccgttgtt
    20101 cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg
    20161 tcagttccgc attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg
    20221 gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga
    20281 ctaattaatg cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga
    20341 aaattcagta ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg
    20401 aactcccgat cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gctatcacct
    20461 gtgcattact ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga
    20521 aaagccgcct ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg
    20581 gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat
    20641 tcaactggca tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt
    20701 ggttctataa cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta
    20761 atacgccgtt tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca
    20821 aacagaatgg cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt
    20881 tcaccattaa cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg
    20941 atcagcgcag cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat
    21001 ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga
    21061 gtgagcaatc tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg
    21121 cggccagaaa atttagcaca gtatatcggt cagcaacatt tgctggctgc ggggaagccg
    21181 ttgccgcgcg ctatcgaagc cgggcatttg cattctatga tcctctgggg gccaccgggt
    21241 accggcaaaa caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt
    21301 atttctgccg tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa
    21361 aaccgcaatg caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa
    21421 agccagcagg atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca
    21481 accactgaaa acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat
    21541 ctgttgaaat ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac
    21601 aaaacccgtg gctatggtgg tcaggatatt gttctgccag atgatacacg acgtgccatt
    21661 gcagaactgg tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat
    21721 atggccgaag tctatgatag cggtaagcgg gtcctgaagc ctgaattact gaccgaaatc
    21781 gccggtgaac gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctaatttcc
    21841 gcactgcata agtcggtacg tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga
    21901 attattaccg ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct
    21961 gaagacgtcg gtaatgccga tccacgggcg atgcaggtgg caattgcggc ctgggattgc
    22021 tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg
    22081 gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct
    22141 cgcgaacgcc cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg
    22201 aaggaaatgg gctacgggca ggaatatcgt tatgctcatg atgaagcaaa cgcttatgct
    22261 gccggtgagg tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac
    22321 aggggccttg aaggcaagat tggtgaaaag ctcgcctggc tggctgaaca ggatcaaaat
    22381 agccccataa aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg
    22441 tggtcgcagg ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc
    22501 gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc
    22561 tttaagctgg atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc
    22621 aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa
    22681 gcgcgcgggg aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg
    22741 gatgcagcaa aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc
    22801 atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa
    22861 gtcagccgct ggggtacccc gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg
    22921 ggtgaaatgc actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt
    22981 gtggtaatga aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat
    23041 ctgcataccg aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac
    23101 acgctgtacg gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg
    23161 ctggaagaag aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg
    23221 actaacctgg tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgaccgcc
    23281 cacaccccat gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc
    23341 cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg tgcgcccaga agactcaatg
    23401 gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg
    23461 taccgtaaaa tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac
    23521 ctggaagtat ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt
    23581 tgggatttcc aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc
    23641 cgtctggttc ataccctgaa cggttctggt ctggctgttg gtcgtacgct ggttgcagta
    23701 atggaaaact atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat
    23761 atgaacggac tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgccttt
    23821 agggcgcttt ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa
    23881 tactactttc gagtgaaaat ctacctatct ctttgatttt caaattattc tatgtataca
    23941 agcctatata gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa
    24001 tcgtgatcaa gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg
    24061 ctgaggagag tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca
    24121 gcagcgcatt aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa
    24181 caaaatcaga cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc
    24241 cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacgaca ataccggcga
    24301 tgacaattac gacggcctac actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtca
    24361 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    24421 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    24481 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    24541 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    24601 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    24661 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    24721 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    24781 gacttactat cttgaacagg cacgccagaa atctaatgct cgcatgatca tcatcgatcc
    24841 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    24901 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actaaaaacc tggtggatca
    24961 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    25021 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    25081 accggaatgg gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    25141 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    25201 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    25261 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    25321 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    25381 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    25441 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    25501 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    25561 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    25621 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    25681 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    25741 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    25801 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    25861 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    25921 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    25981 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    26041 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    26101 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    26161 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    26221 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    26281 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    26341 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    26401 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    26461 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    26521 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    26581 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    26641 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    26701 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    26761 tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg tatggcgcac
    26821 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    26881 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    26941 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    27001 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgaccgggg
    27061 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    27121 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    27181 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    27241 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    27301 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    27361 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    27421 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    27481 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    27541 agcatctgga cgccgatggg ctgggcgatg cgtctgctgc cagccatttc tgtactggca
    27601 ctggtggtga gtggcgtgat gtcagtgatg cagggcacag agctggcaac tattcatagt
    27661 tctgtgcagc aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc
    27721 gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct
    27781 gcggttccgc tactatccgt ctcattcatt ctgcttctgg caggggaatt aatcggtcgc
    27841 ggcgtattct atggtttgca tatgaccgtg gggatggccg tcgcaagcta atcagtaaca
    27901 accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc ataactggtc
    27961 atcaaattac gataatcagg aatatggtta gagaacaacg tcgccagtcc ttcaatatca
    28021 ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacca
    28081 gcttgcgaca tgcgatccca cgccgaatgc cgggtaattt cattaaaggt ccctgatgca
    28141 tcagtcacga caaagacatc aaacccttct tcaatggctg aaagcgccgg gaatgccacg
    28201 caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgactgct
    28261 tttacaaaat cttcgttatc ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca
    28321 tcggggaatt gtgctttcag ttctagaact aatgggccgt taggaccggt ttcaaaactg
    28381 gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta
    28441 tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca
    28501 acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc
    28561 tcctttcgag agtcagtggt acgtctgaaa tcggtcagac gcgatgcatt gctctgaaag
    28621 catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat
    28681 gaaatttgtg ttagatcaat aaaaacaatc agatgagatt aatcatctct tcagcattag
    28741 cgttatgaat gagtaatata aatcgataat accgccgctg cgatatgcgc gtattttcac
    28801 ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa atttgaactt cctcacggtt
    28861 ttaattcatg tccacgtata cccggcctgt catgcttttg ctgtctggcc tgcttttgtt
    28921 gactctggcg atcgcggtgt taaatacact cgtgccgctt tggctcgccc aggaacacat
    28981 gtccacatgg caggtaggcg ttgtcagctc atcctatttt accggcaacc ttgtcggtac
    29041 attgctgaca gggtatgtca ttaaacgcat tggctttaac cgcagctatt atctgtcctc
    29101 cttcattttt gctgctggct gtgccggcct tggcctgatg attggtttct ggagctggtt
    29161 ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt tgggtggttg ttgagagcgc
    29221 gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg cttgctgcgt atatgatggt
    29281 ttattacgtg ggaacgtttt taggccagtt actggtcagc aaagtctcaa ccgagctgat
    29341 gagcgtattg ccgtgggtta caggtttgac attggcaggg atcttaccgc tgttgtttac
    29401 gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg tcaattacgg caatgctaaa
    29461 actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc
    29521 tctatatggc ctgatgcctc tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg
    29581 tttctggatg gcggtactgg tcagtgcggg tatcctcgga caatggccga ttggacgtct
    29641 ggcggataag tttggtcgac tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg
    29701 cagtattgcg atgcttagcc aggcggcgat ggccccagcg ttattcatcc tcggtgccgc
    29761 tggctttacg ctatatccgg tggcgatggc ctgggcttgt gagaaagttg aacatcatca
    29821 actggtgg
//
LOCUS       DAJVPT010000042        29447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVPT010000042 DAJVPT010000000
VERSION     DAJVPT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..498
                     /locus_tag="OQM47_001853"
     CDS             <1..498
                     /locus_tag="OQM47_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1480036.1"
                     /translation="ETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIY
                     ESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPL
                     NKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTI
                     YPIEK"
     gene            520..1929
                     /locus_tag="OQM47_001854"
     CDS             520..1929
                     /locus_tag="OQM47_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480037.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1907..2686
                     /locus_tag="OQM47_001855"
     CDS             1907..2686
                     /locus_tag="OQM47_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1480038.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2683..3543
                     /locus_tag="OQM47_001856"
     CDS             2683..3543
                     /locus_tag="OQM47_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1480039.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3691..4266)
                     /locus_tag="OQM47_001857"
                     /pseudo
     CDS             complement(3691..4266)
                     /locus_tag="OQM47_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4283..4543)
                     /locus_tag="OQM47_001858"
     CDS             complement(4283..4543)
                     /locus_tag="OQM47_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1480040.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4534..5805)
                     /locus_tag="OQM47_001859"
     CDS             complement(4534..5805)
                     /locus_tag="OQM47_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1480041.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5883..6245)
                     /gene="queD"
                     /locus_tag="OQM47_001860"
     CDS             complement(5883..6245)
                     /gene="queD"
                     /locus_tag="OQM47_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1480042.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6564..8363
                     /gene="cysJ"
                     /locus_tag="OQM47_001861"
     CDS             6564..8363
                     /gene="cysJ"
                     /locus_tag="OQM47_001861"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1480043.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPMIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8363..10075
                     /gene="cysI"
                     /locus_tag="OQM47_001862"
     CDS             8363..10075
                     /gene="cysI"
                     /locus_tag="OQM47_001862"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1480044.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10149..10883
                     /gene="cysH"
                     /locus_tag="OQM47_001863"
     CDS             10149..10883
                     /gene="cysH"
                     /locus_tag="OQM47_001863"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1480045.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11147..11299
                     /locus_tag="OQM47_001864"
     CDS             11147..11299
                     /locus_tag="OQM47_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1480046.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11493..12584
                     /locus_tag="OQM47_001865"
                     /pseudo
     CDS             11493..12584
                     /locus_tag="OQM47_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12730..13767)
                     /gene="iap"
                     /locus_tag="OQM47_001866"
     CDS             complement(12730..13767)
                     /gene="iap"
                     /locus_tag="OQM47_001866"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1480047.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14019..14927
                     /gene="cysD"
                     /locus_tag="OQM47_001867"
     CDS             14019..14927
                     /gene="cysD"
                     /locus_tag="OQM47_001867"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1480048.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14929..16356
                     /gene="cysN"
                     /locus_tag="OQM47_001868"
     CDS             14929..16356
                     /gene="cysN"
                     /locus_tag="OQM47_001868"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1480049.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16356..16961
                     /gene="cysC"
                     /locus_tag="OQM47_001869"
     CDS             16356..16961
                     /gene="cysC"
                     /locus_tag="OQM47_001869"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1480050.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17011..17334
                     /locus_tag="OQM47_001870"
     CDS             17011..17334
                     /locus_tag="OQM47_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1480051.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17528..17839
                     /gene="ftsB"
                     /locus_tag="OQM47_001871"
     CDS             17528..17839
                     /gene="ftsB"
                     /locus_tag="OQM47_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1480052.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17858..18568
                     /gene="ispD"
                     /locus_tag="OQM47_001872"
     CDS             17858..18568
                     /gene="ispD"
                     /locus_tag="OQM47_001872"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1480053.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18568..19047
                     /gene="ispF"
                     /locus_tag="OQM47_001873"
     CDS             18568..19047
                     /gene="ispF"
                     /locus_tag="OQM47_001873"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1480054.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19044..20093
                     /gene="truD"
                     /locus_tag="OQM47_001874"
     CDS             19044..20093
                     /gene="truD"
                     /locus_tag="OQM47_001874"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1480055.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20074..20835
                     /gene="surE"
                     /locus_tag="OQM47_001875"
     CDS             20074..20835
                     /gene="surE"
                     /locus_tag="OQM47_001875"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1480056.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20829..21455
                     /gene="pcm"
                     /locus_tag="OQM47_001876"
     CDS             20829..21455
                     /gene="pcm"
                     /locus_tag="OQM47_001876"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1480057.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21595..22734
                     /gene="nlpD"
                     /locus_tag="OQM47_001877"
     CDS             21595..22734
                     /gene="nlpD"
                     /locus_tag="OQM47_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1480058.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22797..23789
                     /gene="rpoS"
                     /locus_tag="OQM47_001878"
     CDS             22797..23789
                     /gene="rpoS"
                     /locus_tag="OQM47_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1480059.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23909..24316)
                     /locus_tag="OQM47_001879"
     CDS             complement(23909..24316)
                     /locus_tag="OQM47_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1480060.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24463..25056
                     /locus_tag="OQM47_001880"
     CDS             24463..25056
                     /locus_tag="OQM47_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1480061.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25056..26003
                     /locus_tag="OQM47_001881"
                     /pseudo
     CDS             25056..26003
                     /locus_tag="OQM47_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26276..26839)
                     /locus_tag="OQM47_001882"
     CDS             complement(26276..26839)
                     /locus_tag="OQM47_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480062.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27004..27255
                     /locus_tag="OQM47_001883"
     CDS             27004..27255
                     /locus_tag="OQM47_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1480063.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27257..29353
                     /locus_tag="OQM47_001884"
     CDS             27257..29353
                     /locus_tag="OQM47_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1480064.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7200 a         7296 c         7975 g         6976 t
ORIGIN      
        1 gaaacctggt ttaacaacct gaactgggga ccggataaag tggctgccga acgtgtgcag
       61 atcctcaaaa ccggcaacat gggctttacc accgaagtgt ccggctgctg gagctgcatc
      121 cacgaaatct acgaaagcgt tattaaccgt attcgtactg agttcccgca cgccgacgac
      181 atcaccatgc tgggcggtca ctcctctcat agctatcaga acggcaccaa catgtacttc
      241 gtctatgact acaacgttgt tgactgtaag ccggaagagg aaatcgacaa gtaccacaat
      301 ccgctcaaca aaatcatctg cgaagaaacc attcgcctcg gcggttcgat ggtgcaccac
      361 catggcattg gtaaacatcg cgttcactgg agcaagctgg aacacggcag cgcgtgggcg
      421 ttgctggaag ggctgaaaaa gcagttcgat cctaacggca ttatgaatac gggtactatc
      481 tatccgattg aaaaataatg tatcaggcag cgttccgcga tgacggggcg ttgccttttc
      541 ggcttctcag gcgagaagcc gttcttatta ccggacaatg aaggggtaaa gatgaacact
      601 tcaccggtgc gaatggatga tttaccgctt aaccgttttc actgtcgaat tgctacgctc
      661 actttcggcg cacacctgac cgacggttat gttctaggcg tcattggtta cgccattatt
      721 caacttacgc ccgccatgca actgacgccg tttatggcgg gaatgatcgg cggctcggcg
      781 ctccttggtt tgtttcttgg cagcctggtt cttgggtgga tctccgacca tattggtcgg
      841 caaaaaatct tcaccttcag ctttttgctg attacgctcg cttcgttctt gcaatttttt
      901 gccaccacgc cagagcatct tattgggctg cgcattttga tcggcattgg tctgggaggc
      961 gattactcag taggtcacac cttgctggct gaattttccc cgcgccgcca tcgcggtatt
     1021 ttgctgggcg cattcagcgt ggtgtggacc gtaggctatg tgttggcaag tattgccgga
     1081 catcacttta tttccgaaaa cccggaggcc tggcgctggt tgctggcatc ggcagctctg
     1141 cccgcgttgt tgattacgtt attacgctgg ggaacgccag aatcgccacg ttggctactg
     1201 tgccaggggc gttttgcaga agctcacgct atcgtgcatc gctattttgg tccccatgtt
     1261 ttactgggcg atgaagtggt aacggcgacc cataaacaca tcaaaacctt gttctcttcg
     1321 cgttactggc ggcgcacggc gtttaacagc gtcttctttg tctgcctcgt aatcccatgg
     1381 tttgtgattt atacctggct gccaactatc gcccagacta ttggtctgga agatgcgctg
     1441 actgccagcc tgatgcttaa tgcgttgtta attgtgggcg cgctgctggg attagttctg
     1501 acgcacctgc tggcacatcg caaatttttg ctgggaagtt ttttgctgct ggcggcaacg
     1561 ctggtagtaa tggcctgttt gccttccggc agttcattaa cgctgctgct ttttgttctc
     1621 ttcagcacca ccatttcggc agtcagtaat ctggtgggca ttttgcctgc ggaaagtttt
     1681 cctactgaca ttcgctcgct gggagtcggt tttgccaccg ccatgagtcg acttggcgcg
     1741 gcggtaagta ctggcctgct gccgtgggtg ctggcgcagt ggggaatgca agtcacctta
     1801 ttgctcctag cgacagtgtt gttggttggt tttgttgtga cctggctatg ggcaccagaa
     1861 actaaagccc tcccgctggt ggcggcggga aatgtaggag gtgcgaatga acattctgtt
     1921 agcgtttaaa gccgaaccgg atgccggaat gctggcggaa aaagagtggc aggcggcggc
     1981 tcagggtaat agcggaccgg atgtttcgct actgcgaagt ttactcggtg ctgatgaaca
     2041 ggccgctgcc gcgctgctgt tagcgcagag aaaaaacggt acgccgatgt ctttaaccgc
     2101 gttgagcatg ggggatgaac gggcgttgca ctggctgcgc tatctcatgg ctcttgggtt
     2161 tgaggaagct gtattgctgg agacggcggc agatctgcgc tttgcgccgg aatttgtcgc
     2221 tcgccatatt gccgaatggc agcgtcagaa tccgctggat ctgataatta ccggcagcca
     2281 aagcagcgaa gggcagaatg ggcaaacgcc atttttgctg gcggaaatgc tgggctggcc
     2341 ctgcttcacc caggtggagc gtttcactct cgacgcgctg tttatcaccc tcgaacagcg
     2401 tactgaacat gggttgcgct gttgccgggt tcgcttgcct gcggtgattg ccgtgcgtca
     2461 gtgcggtgaa gtggccttgc cagtaccggg aatgcgccag cgaatggctg cggggaaagc
     2521 agaaattatc cgtaaaactg ttgctgccga aatgccagcg atgcagtgtc tgcaactggc
     2581 aagagcagag caacgacgcg gagcgacgct gattgatggg caaacggtag cagaaaaagc
     2641 tcaaaagcta tggcaggact atttacgcca aaggatgcag ccatgaatat cgcaattgtg
     2701 acaataaatc aggagaatgc cgcaatagcg agctggctgg cggcgcagga tttcagcggt
     2761 tgtacgctgg cgcactggca aatagaacct cagcccgtag tggcggaaca ggtgctggat
     2821 gcgatggtgg aacagtggca gcgaacgcct gcggacgttg ttctctttcc gccgggaacg
     2881 tttggcgatg aactgtcgac ccggctggcc tggcgtttac atggtgcaag catctgtcag
     2941 gtgacttcgt tagatattcc aaccgtaagc atgcgcaaat cccactgggg aaatgcgtta
     3001 acggcgacgc tgcaaacaga aaaacgcccg ttatgccttt ccctggcgcg tcaggcggga
     3061 gcagcgaaaa acgccacgtt gccctctgga atgcagcaac tgaacattgt cccaggtgcg
     3121 cttcccgact ggctggttag cacagaggat ctgaaaaatg ttagccgcga cccattagct
     3181 gaggccagac gggtgctggt ggtgggacag ggcggagagg cagataacca ggaaatcgtt
     3241 atgctggcag aaaagctggg agccgaagtg ggctacagtc gggcgcgagt gatgaacggt
     3301 ggcgttgatg ctgaaaaagt gattggtatt tccggacatc tgctggcacc tgaggtgtgc
     3361 attgttgttg gcgcttccgg cgcagcagcg ttaatggcgg gcgtacgcaa tagcaagttt
     3421 atggtggcga ttaatcatga cgccagcgca gcggtatttt cacaggcaga tattggcgtg
     3481 gttgatgact ggaaagtggt gctggaggca ctggtgacaa acattcacgc agaataccaa
     3541 taacgtgctg ccggatgatc ctgcctacgg ttctggactg gggacctgta ggcctgataa
     3601 gacgcgttaa gcgtcgcatc aggcgctgaa tgtcggatgt ggcgtgcggc caacaaatca
     3661 aaacaaattc aattggttac gattatcccg tcaaagcaat tttttcgcta acgtccagac
     3721 tccggtattc gtggtggaaa gcgccacgac acccgcgtta atcgcattac gcgcatcttc
     3781 ttcatcgcat accagcccgc cagcaatcag cggttggcgg attttttccg tcacccagcc
     3841 cagcactttg ggcatgcagc ctggcaggat ctcaatacaa tccggattcg attgcgcaac
     3901 ttacttatca atgttgtgaa acgaaatcga atcaacaata aacaggtgat gaatacagaa
     3961 aaaaacttcc gctcttgccg cttttagcat cgatgctttg gtgctgataa ttccatccgc
     4021 ctcggtcacc agtttcaaaa actgaatcac cacttcttta tttgacgcgc cttccagcaa
     4081 atcaacatga ataaaagcat atttcccggc gttcttaatc tttttaacga tattactgat
     4141 ggtgcagata ttgccgtaca acacggaaat aaactggcat tcagaatcga tggccagttg
     4201 caggctggca ttatctttaa cggcagcaat caccggattc tggcggagca ggtgtaagag
     4261 gggcataaat tatccttatt atttatccaa agcggtaggt gatgccgaat ccggaaggcg
     4321 gatagcgcca ctgttgtagt gtggattcgt cgcacagcaa acggcaggtg ccacactcca
     4381 ggcagccgcg atagtcaata cgtaagtcac cttcctgtgt gagagaaaaa agacctaccg
     4441 gacaggcgtt aatcaaccgt tgtaccatct gacgctcaac ggagtcagcc ggaacaatgt
     4501 gcggcgcatc agcaacgcgc cagagattac gggctacaga catcgcagac tcctgataac
     4561 atcgcccgcc agatgccaca ggccatgacg acgtaaatga tgccagaata actggcgcag
     4621 cggtggaaca ggtttatcac cctgatccca taaatcgcgg gaaatatcct gcattaacgc
     4681 aggccacgcc cgatacctgc cagggcgttg caaaagcgcc ggaacatgct gataacgttg
     4741 tagaacatcc catagcaggc tgcgctctac gttgtgatga taaagcgcaa acagattttg
     4801 cggctcgcgg tgctggcagg cgcttattag cgtttgtgcc gccgcctgtg cgccagtcag
     4861 cgccatatcc atgccgcgca cggaaattcc ggtattgacg caactgcgca acgcatcgcc
     4921 caccagcagc cagccgttac cggcgtattg caccggcata ctgtgcaagc caccttctgg
     4981 caccagatgc gcaccatact ccagtgattc cgtgtttttg ataagcgtgc gcactgccgg
     5041 atgcgcttta aagcgagtca gcagctcgct tgccggaaca cgactttgcg taagggaaga
     5101 gagcgggcaa acaatcccta acgagagcgt ttgttgatta gtataaagaa atgcgccgcc
     5161 gggtaagtca tcacagattc cgccgctgaa cagcaacgct gcgccttcgt tattctccag
     5221 atgaaaacgt tcttcaatag cggatgtttc cagcgacagc acttctttga tccccaacgc
     5281 catcgcttcg ccagcaggac gagtcactaa cccgtgacgt tcagccagga cgctgttggc
     5341 accttctgcc agcaccacat aacgggcgcg gagaatatcg tcaccacaaa taacgccaca
     5401 gactctgccg ttttcttcat acagtgcatc caccgtcgct ccggggatgc attcgacacc
     5461 ttctttttcg gcttcggcaa ccagccacgg atcgaaccgt gcacgtaata cattccagga
     5521 ttcaccgccg ggctgtaagc tggaaaacgt cgttgcgcca tccggcgtta acagggaaag
     5581 gctttcgtga gtgatgcgtc gttcaagagg cgcggtcaga tgaaattgcg ggaggagttc
     5641 cgcgagtgca tgggtatata accgcctgcc ggaaagattt ttgctgccgg ggatttcagc
     5701 gcgttcgagt aacaaaacgg ataaatccgc tcgcgcgcag cgtaacgcgc aagcggtgcc
     5761 tgcaataccg gcaccaataa taataatgtc gcagtcgtct tccatgtcat gccccagata
     5821 tccagaacac aacaccctaa catagcgtta ctaaagggaa attgaccgcc gacacactct
     5881 tatcattcgc cgcgatagat acaccctgcg gtgcaggttt cttttaccat caccgcactt
     5941 aacagcggca caacgggttt aacctgatcc caaatccatt ttgctaaaac ttcgctggtt
     6001 ggattttcca gacctggaat atcattgaga taatggtgat cgaggcgctc ataagttggc
     6061 ttaaacgccg ctttcagttc agcgaaatcg ataatccagc ccgtatgcgg atcgacttcc
     6121 ccggtaattt ccagtcgcac cataaaggaa tgcccgtgca ggcgaccaca tttatgccct
     6181 tccgggacat gtggtaagcg gtgagcggct tcgaaggtga aatctttaaa taacgtggtg
     6241 gacatcataa tttctctaca ggacgataaa accgccgtag agtaccggaa aggctgtttt
     6301 ttcgcattat ctaaaacgac tttgttgcgc aaaatcgctg atttatctta atgattggct
     6361 aaattcattt gtttttcatt aggttggtta atctattttg ttgttaaaga ctattgctaa
     6421 aacaggttag tcgatttggt tattagttat cgctatcccg tctttaatcc acaccgttca
     6481 ccccgttaac cttaccttct cttctgtttt atgggcgctg acagggcgca gaaacagctt
     6541 tgcttactgg aacataacga cgcatgacga cacaggtccc accttccgcg ttgcttccgt
     6601 tgaacccgga gcaactggca cgccttcagg cggccacgac cgatttaacg cctacccagc
     6661 ttgcctgggt ttctggctat ttctggggcg tgctcaacca gcagcctgtt gcgcttgcag
     6721 tgacgccagc gcccgccgca gaaatgccgg gtataacgat tatctctgct tcgcaaaccg
     6781 gcaatgcgcg ccgggttgct gaagcattac gcgatgattt attagcagca aaactgaacg
     6841 ttaagctggt gaacgcgggc gactataaat tcaaacaaat cgccagcgaa aaactgctca
     6901 tcgtagtgac gtcaacgcaa ggggaagggg aaccgccgga agaagccgtc gcgctgcata
     6961 agttcctgtt ctccaaaaaa gcgccaaagc tggaaaacac cgcgtttgcc gtgtttagcc
     7021 tcggcgatag ctcttatgaa tttttctgcc agtccgggaa agattttgac agcaagctgg
     7081 cggaactggg gggtgaacgc ctgctcgacc gtgtcgattc tgatgttgaa taccaggctg
     7141 ctgccagcga gtggcgcgcc cgcgtggttg atgcgcttaa atcgcgtgcg cctgtcgcgg
     7201 caccttcgca atccgtcgct actggcgcgg taaatgaaat ccacaccagc ccgtacagca
     7261 aagacgcgcc gctggtggct agcctctctg ttaaccagaa aattactggg cgtaactctg
     7321 aaaaagacgt tcgccatatc gaaattgact taggcgactc gggcctgcgt taccagccgg
     7381 gtgacgcgct gggcgtctgg tatcagaacg atccggcact ggtgaaagaa cttgtcgaac
     7441 tgctgtggct gacaggcgat gaacctgtca ctgtcgaggg caaaacgttg cctctgaacg
     7501 aagcgctaca gtggcacttc gaactgaccg tcaacaccgc caacattgtt gagaattacg
     7561 ccacgcttac ccgcagtgaa acactgctgc cgctggtggg cgataaagcg aagttacagc
     7621 attacgccgc gacgacgctg attgttgaca tggtgcgttt ctccccggca cagcttgatg
     7681 ccgaagcgct aattaatctg ctgcgcccgc tgaccccgcg tctctattcc attgcctcct
     7741 cgcaggcgga agtcgagaac gaagtacacg tcaccgttgg tgtggtgcgt tacgacgtgg
     7801 aaggccgcgc ccgtgccggt ggtgcctcca gcttcctcgc tgaccgcgtg gaagaagagg
     7861 gcgaagtccg cgtatttatc gaacataacg ataacttccg cctgcccgct aacctggaaa
     7921 ccccgatgat tatgattggc ccaggcaccg gtattgcgcc gttccgcgcc tttatgcagc
     7981 aacgcgccgc tgacgaagcg ccaggtaaaa actggctgtt ctttggcaat ccgcatttta
     8041 cggaagactt cctgtatcag gtggagtggc agcgctacgt caaagagggt gtgctgacgc
     8101 ggatcgatct cgcctggtcg cgcgaccaaa aagaaaaagt ttacgtacaa gataaactgc
     8161 gcgaacaggg cgcggagctg tggcgctgga tcaatgacgg tgcccacatt tatgtctgcg
     8221 gcgacgctaa tcgcatggcg aaagacgttg agcaggcact tctggaagtg attgccgaat
     8281 ttggtggcat ggacaccgaa gcggcggatg aatttttaag tgagctgcgc gtagagcgcc
     8341 gttatcagcg agatgtctac taatgagcga aaaacatcca gggcctttag tggtcgaagg
     8401 aaaactgaca gacgccgagc gcatgaagct tgaaagcaac tacctgcgcg gcaccattgc
     8461 ggaagattta aacgacggtc tgaccggcgg ctttaagggc gataacttcc tgctgatccg
     8521 cttccacggc atgtatcagc aggatgaccg cgacatccgc gccgaacgtg ctgaacagaa
     8581 gctggagccg cgccacgcga tgctgttgcg ctgccgtctg ccgggtggag ttatcaccac
     8641 caaacagtgg caggcgatcg ataaatttgc cggtgaaaac accatctatg gcagcattcg
     8701 cctgaccaac cgccagacgt ttcagttcca cggcattctg aaaaagaacg tcaaaccggt
     8761 gcaccagatg ctgcactcgg tcggtcttga tgcgctggcg actgctaacg acatgaaccg
     8821 taacgtactc tgcacctcga acccttacga gtcgcagctg cacgcagaag cgtacgagtg
     8881 ggcgaagaag atttctgaac atctgctgcc gcgtacccgc gcgtatgcgg agatctggct
     8941 cgatcaggaa aaagtcgcca ccaccgatga agaaccgatc cttggtcaga cttacctgcc
     9001 gcgtaagttc aaaaccacgg tagtgatccc gccgcagaac gatattgatc tgcacgccaa
     9061 cgacatgaac ttcgtggcga tcgcggaaaa cggcaagctg gtgggcttca acctgctggt
     9121 gggcggtggg ctttctatcg aacacggtaa caagaaaacc tacgcccgca cggcgagtga
     9181 gtttggctat ctgccgctag agcatacgct agcggtggcg gaagccgtcg tgacaactca
     9241 gcgtgactgg ggtaaccgaa ccgatcgtaa aaatgccaaa accaaataca cactggagcg
     9301 cgtgggggtt gagacgttta aagcggaagt ggagcgtcgc gcggggatca aatttgaacc
     9361 gatccgcccg tatgaattta caggacgcgg cgatcgtatt ggctgggtta agggcattga
     9421 tgataactgg cacctgacgc tgtttatcga aaatggtcgc atccttgatt atccggggcg
     9481 tccgctgaaa actggcctgc tggagatcgc gaagatccac aaaggtgatt tccgcattac
     9541 ggcgaaccag aatctgatca tcgccggtgt gccggaaagc gagaaagcga agatcgagaa
     9601 aattgccaaa gagagcgggt taatgaatgc cgtcacgccg cagcgtgaaa actcgatggc
     9661 ctgcgtgtca ttcccgactt gcccgctggc gatggcggaa gcggagcgtt tcctgccgtc
     9721 ttttatcgac aacatcgata atttaatggt gaaacatggt gtcagcgatg agcatatcgt
     9781 gatgcgtgta acaggctgcc cgaacggttg tggtcgcgcg atgctggcgg aagtgggcct
     9841 ggtgggtaaa gcaccgggtc gctacaacct gcatcttggc ggcaaccgca ttgggacacg
     9901 tatcccacgg atgtataaag aaaacatcac cgagccggaa atcctggcgt cgcttgatga
     9961 actgataggg cgctgggcga aagagcgcga agcgggtgaa ggcttcggcg actttacggt
    10021 gcgtgcgggc atcattcgcc cggtgctcga tccggcgcgc gatttgtggg attaaccatc
    10081 agcccggtct tgtaggcctg ataagacgcg tgagcgtcgc atcaggcaag gcaaacagtg
    10141 aggaatctat gtccaaactc gatctaaacg ccctgaacga actgccgaag gtagatcgca
    10201 ttctggcgct ggcagaaact aacgccgaac tggaaaaact ggacgctgaa ggccgcgttg
    10261 cctgggcgct ggataatctg cccggtgaat atgtactttc ttccagcttt ggcattcagg
    10321 cggcggtgag cctgcatctg gtgaatcaga ttcacccgga tattccggtg atcctcaccg
    10381 acaccggcta tctgttcccg gaaacctacc gctttattga cgagttaacg gacaaactca
    10441 agctcaacct gaaagtgtac cgcgctaccg aaagcgccgc ctggcaggaa gcacgctacg
    10501 gcaaactgtg ggaacagggc gttgaaggca ttgaaaagta caatgacatc aacaaagtcg
    10561 aaccgatgaa ccgggcgctg aaagaactga atgcgcaaac gtggtttgct ggcctgcggc
    10621 gtgaacaatc cggcagccgt gccaatttac cggtgctggc aattcagcgt ggcgtattta
    10681 aagtgctgcc gattatcgac tgggataacc gaactattta tcagtacctg caaaaacatg
    10741 gcctgaaata tcacccatta tgggacgaag gatatttatc ggtgggcgat acccatacaa
    10801 cccgtaaatg ggaacccggc atggcggaag aagaaacgcg tttctttggc ttaaaaaggg
    10861 aatgtggatt acacgaaggg taaatattgc cggataaatt aaacgcctct gtcagaaatg
    10921 atgggggcgt ttttattttg cgagcagcat tccggcattt aaaggtgaac atcgcaggga
    10981 aaattaacag gtatagaatg gcgttgggtg cttgaggctg tctggcgtta agcattcgcg
    11041 aggttccaga tggacaaaag ccccaggcga tatttctatc aacctgaggc tcgcgttcga
    11101 acgtagcaat tcggatgtta gtctcttctt tgcagggagg caagacatgc tgacaaaata
    11161 tgcccttgtg gcaatcatcg tactgtgttg tacagtactg ggattcacgt tgatggtagg
    11221 tgactcgtta tgtgagttga gtatcagaga acgtggtatg gagtttaagg cagttctcgc
    11281 ttacgaatcg aagaagtagc caccacgcgg gggtaaaacc ccgcgtactg aattgtgatg
    11341 gtttggtctc ctgcaccctt tttttaaatt tatatttttt atgtgaatat atagtctcta
    11401 tttattatta tgaatttcaa atgaaaatca ttaatttatt cataatgtaa atgttttatt
    11461 ggctgatata tatcatagat agtttttaaa atatgcgtaa atatccttta agtttactga
    11521 aggataaaaa tattgtgact ttctttgatt tctggggaaa aaaccgacgt ggcgagaaag
    11581 agggtatcga cgactatcac cttctttgct ggcatttgct ggatgtggcc gcaatgggct
    11641 atttaatggt taaaagaaat tgcttcgggc tggctgatta ttttcgtcaa ttagggattt
    11701 ctgacaagga acaggcagct cgatttttcg cctggttgct gtgctggcac gatattggaa
    11761 aatttgcccg ctcttttcaa caactttacc tgccccctga attcaagatt caggaaggtg
    11821 ccagaaaaaa ttacgaaaaa atctctcatt caacgctggg ctactggttg tggaatcatt
    11881 atttaagtga atgtcaggag ttacttcctt catcttcgct ctctccgcgc aaacttaggc
    11941 gagtgataga aatgtggatg cccgtgacga cagggcatca tggtcggccc cctgatcgta
    12001 tggatgaatt ggataatttt ctgcctgaag acaaagctgc agcacgagat tttctccttg
    12061 aaatcaagcc actgtttccg cgaatagaga tccccacatt ctgggatgat gacgagggcg
    12121 ttgaacttat aaaacaactt tcctggtata tctctgcaac cgtcgtactt gcagactgga
    12181 cagggtcgtc aacgcgattt tttccacgcg tcgcacaggc aatggatatt gaagattact
    12241 ggcaaaaggc tttagttcag gctcaaaacg cattaaccgt ctttccgcca caagcagaaa
    12301 ccgcgccttt caccggaatt aatacgctgt ttccttttat tgagaatacg acaccgttac
    12361 agcaaaaggt gctggatctg gatatcaatc agccaggacc acagttgttt attctggaag
    12421 atgtgaccgg cgcaggtaaa acagaagcgg cgcttatcct ggcgcacagg ttgatggctg
    12481 cgggtaacgc tcaggggctg ttttttggcc tgccaacaat ggcaacggcc aatgccatgt
    12541 acgatcgact ggtcaaaacc tggcttgctt tctattcgcc agaggtgttc cccgctccag
    12601 cggagataaa ccggcgcaat acggtgcggt tttcccggaa gtaaaccgga cgcactggat
    12661 gcgatgatgg gtatcactta gaattccccg cccctgcggt agaactccca gctcccattt
    12721 tcaaacccat caagacgcct tcgccagctc cttcaccaac ggtagcatta tccgcataac
    12781 gtcacggcag cgacgttcta ttcttccagg aagtgcctta tcaatatgtt gttgattatc
    12841 cagtcttacg tcatgccagc tatttcccgc cgggaaggca ggtgtttttg cgcgttgctg
    12901 ataaccatcc ttatttccaa gattccagtt agtcgcttcc accgaaagta cagcaatgcc
    12961 cgctttgtcg aatatttctg cgtcattaca acccccagtg cctttcggat aatttttatt
    13021 caaacccgga ttggtcgttg cggctattcc gtgactgcgt gcaattgcca gcgccctgtc
    13081 gcgcgttaat ttccttactg cttcaggggt ttttacaccg ctgttgaaat acaatttatc
    13141 gccaacaatt aagttatcga gattaatcac cagcagcgta tttttctttt cggtgtcact
    13201 catccgcttg agtaaattct cagcgcctaa ttttccttcc tcttcgccgc tggtcgccac
    13261 aaatcgaata ccatactcgg taggcgtatt tttcaggcgt tctgccagtt ccagcatgac
    13321 acctaaacct gcggcgttat catctattcc ttgtaacgtc agtccgccga gattggcatc
    13381 tgcgtcagca tcgctcagcg gggcgtaagt atccagatgc gccataatga tgatctgctg
    13441 cggcgctttg ccttcatgag cggcaatcac cgtacttcct gtcacgttat gccagttctt
    13501 gcgattatcg cgggcggtat aaatataccg actattaaat gtccgaatat cactgcgata
    13561 acccatttgc tgaaactgtt ggcgaatata atcggcagat aacatttctg ctggagttcc
    13621 ggtcatgcgt cccggaaaga aagtagcaat atgtcgtgcc tgggtattag caaaatcgcc
    13681 aggtttaggt gacgaggcgt gtacggggag aataaagcat acgccgagcg ccagggcagc
    13741 ggtacggtgg cgcaatgcgg aaaacatagt gagtccttaa ataccatgca aattttttta
    13801 ccgccatagt atgaaactgc cgctgcgcta aaacaatttc aaatcttcct aaacgcccga
    13861 aatccggtgc cttaagcact ttttgatatt agctttgcca aatcgttatt ccgttaagga
    13921 actactcatt ctaattggta atttcattcg ttctcttacg ctccctatag tcgaaacatc
    13981 tgatggcaag aaaatagcgg tattgcaaag gaacggttat ggctcaaaaa cgacttactc
    14041 acctgcggca actggaggcg gaaagcatcc acattattcg cgaggtggcg gcagaattct
    14101 caaatccggt gatgctctac tctatcggta aagattccag cgtcatgctg catctggcgc
    14161 gcaaggcgtt ttatccaggt acgctgcctt tcccgttgct gcatgtcgat accggctgga
    14221 aattccgcga gatgtatgag ttccgcgatc gtacggcgaa agcctacggc tgcgagctgc
    14281 tggtgcataa aaacccggaa ggcgtggcga tggggattaa tccattcgtg cacggcagcg
    14341 cgaaacatac cgatattatg aaaactgaag gcctgaaaca ggcgctgaac aaatacggtt
    14401 ttgatgccgc cttcggtggt gcgcgccgtg acgaagagaa atcccgcgct aaagagcgaa
    14461 tctactcttt ccgtgaccgc ttccatcgct gggatccgaa aaatcagcgc ccggagctgt
    14521 ggcacaacta caacgggcaa attaacaaag gcgaaagcat ccgcgtcttc ccgctctcta
    14581 actggaccga gcaggatatc tggcaataca tctggctgga aaatatcgac attgtgccgc
    14641 tgtatctcgc tgcggaacgt ccggttctgg aacgcgacgg tatgttgatg atgattgatg
    14701 acaaccgtat cgacctgcaa ccgggcgaag tgatcaaaaa acggatggtg cgtttccgta
    14761 cgctgggctg ctggccgctg accggtgcgg tggagtcaaa tgcacaaaca ctgccggaaa
    14821 tcatcgaaga gatgctggtt tccaccacca gtgaacgtca gggtcgcgtg attgaccgcg
    14881 accaggcggg atccatggag ctgaaaaaac gtcaggggta tttttaagat gaacaccgta
    14941 cttgcacaac aaatcgccaa tgaaggcggc gtcgaagcct ggatgattgc gcaacaacat
    15001 aaaagcctgc tgcgttttct gacctgtggt agcgtcgatg acggcaaaag taccctgatt
    15061 ggtcgtctgc tgcacgatac ccgccaaatc tacgaagatc agctctcatc gctgcataac
    15121 gacagtaagc gtcacggcac ccagggcgaa aagctggatc tggctctgct ggtggacggc
    15181 ctgcaagctg agcgcgaaca gggcatcact attgatgtgg cctaccgcta tttctctacc
    15241 gagaagcgta aatttattat cgccgacacc ccagggcacg agcagtacac tcgcaatatg
    15301 gcgactggcg catcgacatg tgaactggcg atcttactga ttgatgcccg taaaggcgtg
    15361 ctcgatcaaa cccgtcgtca cagttttatc tccacactgt tggggatcaa acatctggtc
    15421 gtggcgatca acaaaatgga tctggtggat tacagtgaag agacgttcac ccgtattcgt
    15481 gaagattatc tgacctttgc cgggcagctg ccgggtaatc tggatatccg ctttgtgccg
    15541 ctctccgcac tggaaggtga caacgtggct tcgcaaagtg aaagtatgcc gtggtacagc
    15601 ggtctgacac tgctcgaagt gctggaaacc gtggagatcc agcgagtggt ggatgcccag
    15661 ccaatgcgct tcccggtgca gtacgttaac cgcccaaatc tcgattttcg tggctacgcc
    15721 ggaatgctgg catccggtcg cgtggaagtc gggcaacgtg taaaagtgct gccctctggt
    15781 gtggaatcaa acgtcgcgcg gatcgtgact tttgatggtg atcgcgaaga agtctttgcc
    15841 ggagaagcga tcaccctggt gctgacggat gagatcgata tcagccgtgg tgatctgctg
    15901 ctggcggcag acgaagcgtt accggcggtg cagagtgcgt cggtggatgt ggtatggatg
    15961 gcggaacagc cgctttcccc gggccagagt tatgacatca aaattgccgg taagaagacg
    16021 cgtgctcgtg ttgatggcat tcgttatcag gttgatatta ataaccttac ccaacgcgaa
    16081 gttgaaaacc tgccgctgaa cggcatcggc ctggtggatc tcacttttga cgagccactg
    16141 gtgttagatc gctatcagca aaatccggtt accggtgggc tgatttttat cgatcgcctg
    16201 agcaatgtga ccgtgggtgc cggtatggtg cacgagccag ttagccaggc aacagctgcg
    16261 tcatctgaat tcagtgcatt cgaactggaa ttgaatgccc tggttcgtcg ccactttccg
    16321 cactggggcg cgcgcgattt gctgggggat aaataatggc gctgcatgac gaaaacgtcg
    16381 tctggcatag tcatccggtc actgtgcaac aacgcgaact acaccacggt catcgtggtg
    16441 tagtgctgtg gtttaccggc ctctccgggt ccggtaaatc aacggtcgcc ggggcgctgg
    16501 aggaggcgtt acataaactc agcgtcagta cgtatctgct ggatggcgac aatgttcgcc
    16561 acggattatg cagcgatctc ggttttagcg atgccgatcg taaagagaat atccgtcgcg
    16621 tcggtgaagt ggcgaatttg atggttgaag ccggactggt ggtgctgacc gcatttatct
    16681 cgccacaccg cgccgaacgc cagatggttc gcgaacgcgt aggagaaggg cgctttattg
    16741 aagtgtttgt cgatacgccg ctggcgattt gcgaagcccg cgatccaaaa ggcttatata
    16801 agaaagcgcg tgccggtgaa ctgcgcaact ttacgggaat agattccgtt tacgaagcgc
    16861 ctgaatcggc agaaattcat ctcaatggtg aacaattagt aacaaatttg gtacagcaat
    16921 tattagatct gttgagacag aacgatatta tcagatcctg agacaccacc gggtttccat
    16981 gcccggttcg tcaaagtaac aggatcaaat atgcgtaata gccataacat tacactaaca
    17041 aataacgaca gccttactga ggatgaagaa actacatggt cactgcctgg tgccgtggtc
    17101 ggttttatct cctggttatt tgcgctggcg atgccgatgt tgatttatgg ctctaacacg
    17161 ctgttcttct ttctctacac ctggcctttc tttctggcac tgatgcctgt cgcggtagtg
    17221 gtggggattg cgctgcattc attgatggac ggaaagctac gctacagtat tgttttcacc
    17281 ctggtgacag taggcattat gtttggcgca ctgtttatgt ggctactggg ctaatttgta
    17341 ctttcttgtc tcctctgttc ataattcaaa ccgtaactaa taatgagatt atgttctgca
    17401 cgccctgggt atacgtaaca atgggcaaat gtggtacatt tgcccgcgtt gtcgcggtat
    17461 ccccaacaga ggatgtagag tcgtcttcgg atgcatggga tgatgatgca gtttttcagg
    17521 gggcaggatg ggtaaactaa cgctgctgtt gctggctatt ctggtctggc tacagtattc
    17581 gctgtggttc ggtaagaacg gtatacatga ctatacccgc gtcaatgatg atgtggcggc
    17641 actgcaagct acaaacgcga aacttaaagc gcgaaacgat caactttttg ccgaaattga
    17701 cgatctcaat ggcggccagg aggcgctcga agagcgtgcg cgtaatgaac tcagcatgac
    17761 caggccgggc gaaacttttt atcgtctggt gcctgacgcg tcgaagcgcg cacagtctgc
    17821 ggggcaaaac aatcgataaa tcagcccggg aattaacatg gcaaccactc atttggatgt
    17881 ttgcgccgtg gttccggcgg ccggatttgg ccgtcgaatg caaacggaat gtcctaagca
    17941 atatctctca atcggtaatc aaaccattct tgaacactcg gtgcatgcgc tgctggcgca
    18001 tccccgggtg acacgtgtcg tcattgccat aagtcctggc gatagccgtt ttgcacaact
    18061 tcctctggcg aatcatccgc aaatcaccgt tgtagatggc ggtgatgagc gtgccgattc
    18121 cgtgctggca ggtctgaaag ccgctggcga tgtgcagtgg gtattggtgc atgacgccgc
    18181 tcgtccttgt ttgcatcagg atgaccttgc gcgattgttg gcgttgagcg aaaccagccg
    18241 cacgggaggg atcctcgccg caccagtgcg cgatactatg aaacgtgccg aaccgggcaa
    18301 aaatgccatt gctcataccg ttgatcgcaa cggcttatgg cacgcgctga cgccgcaatt
    18361 tttccctcgt gagctgttac atgactgtct gacgcgcgct ctaaatgaag gcgctaccat
    18421 taccgacgaa gcctcggcgc tggaatattg cggatttcat ccgcagttgg tcgaaggccg
    18481 cgcggataac attaaagtca cgcgcccgga agatttggca ctggccgagt tttacctcac
    18541 ccgaaccatc catcaggaga atacataatg cgaattggac acggttttga cgtacacgcc
    18601 tttggcggtg aaggcccaat tatcattggt ggcgtacgca ttccttacga aaaaggattg
    18661 ctggcgcatt ctgatggcga cgtggcgctt catgcgttga ccgatgcatt gcttggcgcg
    18721 gcggcgctgg gggatatcgg caaactgttc ccggataccg atccggcatt taaaggtgcc
    18781 gacagccgcg agctgctacg cgaagcctgg cgtcgtattc aggcgaaggg ttataccctt
    18841 ggcaacgtcg atgtcactat catcgctcag gcaccgaaga tgttgccgca cattccacaa
    18901 atgcgcgtgt ttattgccga agatctcggc tgccatatgg atgatgttaa cgtgaaagcc
    18961 actactacgg aaaaacttgg atttaccgga cgtggggaag ggattgcctg tgaagcggtg
    19021 gcgctactca ttaaggcaac aaaatgattg agtttgataa tctcacttac ctccacggta
    19081 aaccgcaagg caccgggctg ctgaaagcca atccggaaga ctttgtggtg gtggaagatt
    19141 tgggctttga gcctgatggt gaaggtgagc atattctggt tagaatcctc aaaaacggct
    19201 gcaatacccg ttttgtggcg gatgcactgg cgaaattcct gaaaattcat tcccgtgaag
    19261 tcagcttcgc tgggcaaaaa gacaaacatg ctgtaacgga acagtggtta tgtgctcgcg
    19321 tgccgggcaa ggaaatgccc gatctgagcg cctttcaact ggaaggctgc caggtgctgg
    19381 agtatgcgcg gcacaagcgc aagctgcgtt taggggcgct gaaaggtaac gcctttaccc
    19441 tggttctgcg cgaagtgagc aatcgcgatg acgttgaaca acgtctgatc gatatttgcg
    19501 taaaaggtgt accgaactac ttcggtgccc aacgttttgg gattggcggt agcaacttgc
    19561 agggcgcgct gcgctgggcg caaaccaata ctccggtgcg cgatcgcaat aaacggagtt
    19621 tttggttgtc ggcagcccgc agtgcgttgt ttaatcagat tgttgctgag cgcctcaaaa
    19681 aagcagacgt taatcaagtt gttgacggcg atgcgctaca attagccgga cgtggtagct
    19741 ggtttgtagc aaccaccgaa gaactggcgg aattacagcg tcgcgtcaac gataaagagc
    19801 tgatgataac cgccgcattg ccgggcagtg gcgaatgggg aactcagcgt gaagcgctgg
    19861 cattcgaaca agcagctgtc gccgcagaaa ctgaattaca agctttactg gtgcgcgaaa
    19921 aagttgaagc cgcgcgcaga gcgatgctgc tgtatccgca acaattaagc tggaactggt
    19981 gggatgacgt caccgtagag atccgtttct ggcttccggc gggtagtttt gcaaccagcg
    20041 ttgtcaggga acttatcaac acaacaggtg attatgcgca tattgctgag taattatgac
    20101 ggggtacatg cacccggtat acaaacgctg gcgaaagcct tgcgtgagtt tgctgacgtt
    20161 caggtggtcg cccccgatcg taaccgcagc ggcgcttcaa attctctgac actggaatcc
    20221 tccctgcgca cgtttacctt tgaaaatggt gatattgctg tgcaaatggg aaccccgacc
    20281 gattgtgtct atcttggcgt gaatgctctg atgcgtccgc gcccggacat tgttgtgtcc
    20341 ggaattaacg ccgggccgaa tctgggggat gatgttattt attccggtac ggtagccgcc
    20401 gcgatggaag gccgtcattt aggttttccg gcgctggccg tctcgcttga cgggcataaa
    20461 cattacgaca ctgccgcggc ggtaacctgt tcaattttgc gcgcactgtg taaagagccg
    20521 ctgcgcaccg gacgtattct taatattaac gttcaggatt tacccttgga tcaaatcaaa
    20581 ggtattcgcg tgacgcgctg cggtacacga catccggcag atcaggtgat cccgcagcaa
    20641 gatccgcgcg gcaatacgct gtactggatt ggcccgccgg gcggtaaatg tgatgccggt
    20701 ccggggaccg attttgctgc ggtagatgag ggctatgtct ccatcacgcc gctgcatgtg
    20761 gatttaactg cgcatagcgc gcaagatgtg gtttcagact ggttaaacag cgtgggagtt
    20821 ggcacgcaat ggtaagcaga cgcgtacaag cacttctgga tcaattacgt gcgcaaggta
    20881 ttcaggatga gcaggtgctg aatgcacttg ccgccgtgcc gcgtgaaaaa ttcgttgatg
    20941 aagcgtttga acaaaaagcc tgggacaata tcgccttgcc gataggtcag gggcagacaa
    21001 tttcgcagcc gtatatggtg gcgcgaatga ccgaattact cgagctgacg ccgcagtcgc
    21061 gggtgctgga aattggcact ggttcgggat atcaaacggc aatcctggcg catcttgtcc
    21121 agcatgtttg ctcggttgaa cggattaaag gcttgcagtg gcaggcacgt cgccgcctga
    21181 aaaatcttga tttacataat gtttcaaccc gtcatggtga tggatggcaa ggttggcagg
    21241 cacgtgcgcc gtttgacgct atcattgtta cggcggcacc gccggaaatt ccaactgcgc
    21301 taatgacgca gctggacgaa ggcgggattc tcgtcttacc cgtaggggag gagcaccagt
    21361 atttgaaacg ggtgcgtcgt cggggaggcg aatttattat cgataccgtg gaggccgtgc
    21421 gctttgtccc tttagtgaag ggtgagctgg cttaaaacgt gaggaaatac ctggattttt
    21481 cctggttatt ttgccgtagg tcagcgtatc gtgaacatct tttccagtgt tcagtagggt
    21541 gccttgcacg gtaattatgt cactggttat taaccaattt ttcctggggg ataaatgagc
    21601 gcgggaagcc caaaattcac cgttcgccgc attgcggctt tgtcactggt ttcgctatgg
    21661 ctggcaggct gttctgacac ttcaaatcca ccggcaccgg tcagctccgt taatggcaat
    21721 gcgcctgcaa atactaattc tggtatgttg attacgccgc cgccgaaaat ggggacgacg
    21781 tctacagcgc agcaaccgca aattcagccg gtacagcagc cacaaattca ggctactcaa
    21841 caaccgcaaa tccagccagt gcagccagta gctcagcagc cggtacagat ggaaaacgga
    21901 cgcatcgtct ataaccgtca gtatgggaac attccgaaag gcagttatag cggcagtacc
    21961 tataccgtga aaaaaggcga cacacttttc tatatcgcct ggattactgg caacgatttc
    22021 cgtgaccttg ctcagcgcaa caatattcag gcaccatacg cgctgaacgt tggtcagacc
    22081 ttgcaggtgg gtaatgcttc cggtacgcca atcactggcg gaaatgccat tacccaggcc
    22141 gacgcagcag agcaaggagt tgtgatcaag cctgcacaaa attccaccgt tgctgttgcg
    22201 tcgcaaccga caattacgta ttctgagtct tcgggtgaac agagtgctaa caaaatgttg
    22261 ccgaacaaca agccaactgc gaccacggtc acagcgcctg taacggtacc aacagcaagc
    22321 acaaccgagc cgactgtcag cagtacatca accagtacgc ctatctccac ctggcgctgg
    22381 ccgactgagg gcaaagtgat cgaaaccttt ggcgcttctg aggggggcaa caaggggatt
    22441 gatatcgcag gcagcaaagg acaggcaatt atcgcgaccg cagatggccg cgttgtttat
    22501 gctggtaacg cgctgcgcgg ctacggtaat ctgattatca tcaaacataa tgatgattac
    22561 ctgagtgcct acgcccataa cgacacaatg ctggtccggg aacaacaaga agtgaaggcg
    22621 gggcaaaaaa tagcaaccat gggtagcacc ggaaccagtt caacacgctt gcattttgaa
    22681 attcgttaca aggggaaatc cgtaaacccg ctgcgttatt tgccgcagcg ataaatcggc
    22741 ggaaccaggc ttttgcttga atgttccgtc aagggatcac gggtaggagc caccttatga
    22801 gtcagaatac gctgaaagtt catgatttaa atgaagatgc ggaatttgat gagaacggag
    22861 ttgaggtttt tgacgaaaag gccttagtag aagaggaacc cagtgataac gatttggccg
    22921 aagaggaact gttatcgcag ggagccacac agcgtgtgtt ggacgcgact cagctttacc
    22981 ttggtgagat tggttattca ccactgttaa cggccgaaga agaagtttat tttgcgcgtc
    23041 gcgcactgcg tggagatgtc gcctctcgcc gccggatgat cgagagtaac ttgcgtctgg
    23101 tggtaaaaat tgcccgccgt tatggcaatc gtggtctggc gttgctggac cttatcgaag
    23161 agggcaactt ggggctgatc cgcgcggtag agaagtttga cccggaacgt ggtttccgct
    23221 tctcaacata cgcaacctgg tggattcgcc agacgattga acgggcgatt atgaaccaaa
    23281 cccgtactat tcgtttgccg attcacatcg taaaggagct gaacgtttac ctgcgaaccg
    23341 cacgtgagtt gtcccataag ctggaccatg aaccaagtgc ggaagagatc gcagagcaac
    23401 tggataagcc agttgatgac gtcagccgta tgcttcgtct taacgagcgc attacctcgg
    23461 tagacacccc gctgggtggt gattccgaaa aagcgttgct ggacatcctg gccgatgaaa
    23521 aagagaacgg tccggaagat accacgcaag atgacgatat gaagcagagc atcgtcaaat
    23581 ggctgttcga gctgaacgcc aaacagcgtg aagtactggc acgtcgattc ggtttgctgg
    23641 ggtatgaagc ggcaacactg gaagatgtag gtcgtgaaat tggcctcacc cgtgaacgtg
    23701 ttcgccagat tcaggttgaa ggcctgcgcc gtttgcgcga aatcctgcaa acgcaggggc
    23761 tgaatatcga agcgctgttc cgcaagtaag taagcatctg tcagaaaggc cagtctcaag
    23821 cgaggctggc ctttttcttt tgtttgggaa gcttgtgttt ttgtcgcctg gataagacac
    23881 gtcagcgtcc tacccggcaa tcagcattct atgtgctcat catcttgcgc accaactgca
    23941 taaacagctc ctgctcttct ccactcaaac gccccaaaaa ttcctcatcc acgctgtcac
    24001 caataggtat cgccgcagca agtagctttt ctccttcggc agtcagccag acaaagcgtc
    24061 gccgcttgtc agcgacgtca tgttcacgtc tgactaagcc acgattttcc attcttgcca
    24121 acatttctgc tagcgttgct ttggtgctga ctgctgcttc tatcagtgcc acctgttcaa
    24181 taccaggttt atcagcaatg gcgcgcatca ccgcgtactg tggtttggtc aggtcaggta
    24241 actcgtgctg ccagcgagcc gtgtgctgct gaaaaagctg tcgtaactga tggaacgctt
    24301 tatttcgtaa cgccatgtaa actccgggct aattctctgt cgctatcata gcggtttttt
    24361 agtgacgaag aagatgttgt tacttttcaa tccttgccgc tgtggaaatg aaggagtatg
    24421 ttaataataa cgttcgcata cgaataatta agagaataag caatgaaact gatcgtcggg
    24481 atgacagggg ctaccggtgc gcctcttggt gtggcattac tgcaagcgct gcgtgagatg
    24541 ccgaatgtcg agactcatct ggtgatgtcg aagtgggcga aaaccaccat tgaactggaa
    24601 acgccttaca gcgctcgcga tgttgctgcc ctcgcagact tcagccataa cccggcggat
    24661 caggcggcga tcatctcatc cggttctttt cgtaccgacg gcatgatcgt tattccgtgc
    24721 agtatgaaaa cgctcgccgg tatccgcgct ggttacgccg atggcctggt agggcgcgcg
    24781 gcggacgtcg tgctcaaaga aggccgcaaa ctggtgctgg tgccgcgtga aatgccgctt
    24841 agcaccatcc atctcgaaaa tatgctcgca ctttcacgca tgggcgtggc gatggtgccg
    24901 ccgatgcctg ccttttataa ccatcccgaa acggtagatg acattgtcca ccatgtggta
    24961 gcccgcgtgc tggatcaatt tggcctcgaa catccccacg ccaggcgctg gcaaggattg
    25021 ccgcaggccc ggaatttttc tcaggagaat gaataatggc atttgatgat ttacgcagct
    25081 ttttacaggc gcttgatgac cacggccagt tactgaaaat cagcgaagaa gtgaacgctg
    25141 agccggatct ggcagcagca gctaacgcca ccgggcgtat cggcgacggc gcgcccgcgc
    25201 tgtggtttga taatattcgc ggctttaccg atgcccgcgt ggcgatgaat accatcggtt
    25261 cctggcagaa ccacgcgatt tcccttggcc tgccaccaaa caccccggtc aaaaagcaga
    25321 ttgatgagtt tatccgccgc tgggataact tcccgattgc cccggagcgc cgcgccaatc
    25381 cagcctgggc gcagaacacc gtcgatggcg aagagatcaa cctgttcgat attctgccgc
    25441 tgtttcgcct gaatgatggc gatggcggtt tctatctcga caaagcgtgc gtggtttccc
    25501 gcgatccgct cgacccggat aacttcggca agcagaacgt cggcatctac cgcatggaag
    25561 tgaagggcaa gcgtaagctc ggcctgcaac cggtgccgat gcacgatatc gccctgcatc
    25621 tgcataaagc agaagagcgc ggtgaagatc tgccgattgc gatcacgctc ggtaacgatc
    25681 cgatcatcac gctgatgggg gccacgccgc tgaaatacga tcagtctgag tatgaaatgg
    25741 caggcgcgct gcgtgaaagc ccgtacccga tcgccaccgc tccgctgacc ggttttgatg
    25801 tgccgtgggg ttcagaagtg atcctcgaag gggttatcga aagccgtaaa cgcgaaatcg
    25861 aagggccgtt cggtgagttt accgggcact actccggtgg gcgcaacatg accgtggtgc
    25921 gcatcgataa agtctcttat cgcagcaaac cgattttcga atcactctat ctcggtatgc
    25981 cgtggaccga aatcgactac ctatgactag gtaatttatt caagataacc gggaagagat
    26041 taaaaaagac gggaatagat tggaatcaac aagttaggaa ctcaaggttt tcaagataaa
    26101 gcgagtccaa aaatgttaaa cggttcgata tcaataaaac attcaagata actcacaggt
    26161 tcttacaata gcctttaaag accgttcaaa aacgattcag gtgtttatta tagcgctgat
    26221 gggtgcaagc gccaaaacca atgtaatttt agcacagcta ggcacttgca ctgggttatg
    26281 cgctggctgc tttctgaggt agatttaagt gatcgccctt agcttcggac aacgcgtaaa
    26341 tctctctaac cttttcgtct ggcaaattac caaggcttaa cgctatggct ctacgaactc
    26401 caactagagc gtgcaactct tggatgtgct catccagaag aatgagtaat tgctccgctc
    26461 cttttctccc aagaacatca gctacttgtt gtttttcttg tggtttaaga aatgacaaga
    26521 acattagaat ttgagcatca acattatccg cattctcttt tttgggttct ttggaatctt
    26581 ttagttcaac atcaccctct cccgtcatga gccattccaa ggtaacattt tcgctttctg
    26641 ctatcttttt taactgatac gggcgtggta tcggtgcgta atcccgcctg ttgtaataat
    26701 tttttaaagt gccttcattg atcccccatg cccgagcagc cgcatttctt gaagggtatc
    26761 gttctactaa cctaaagatt ctttcgataa tcggttcttt tccatcggca ggaaaagaac
    26821 cttggggtgc ttttctcatt cttggttcct ttctatctaa ttcattgata aataataaaa
    26881 ttaaaaaata aaccataaaa acaccaaaga accctttgca gggaatcttt ggtatcttaa
    26941 tctttatcca tacggatatc cgaccggata tccgcgcggg taattttgta ggttaaagga
    27001 aagatgagac aaagagaaga tagtgaccgc gattggcctt cgctgaagat tcagctggcg
    27061 ctcaaagata acgggtggtc ttccttccgg gagctttcac tccatgctgg ttatgctcag
    27121 gattcactga aagcggtagt tcgcacacca aacaaaaaat acgaacgcgt aatttctgac
    27181 gctataggtg taccgccaga aatcatctgg cccagccggt atcgtaacga tggatttatc
    27241 aggaaggcgt cgtgatatgc agtcgtacgt taccgtaaat gacctgctcg gcgtaccagg
    27301 aatgccagcc accacaaaag gcatccgaca ggcgttacag cgtttttctc gtgatcttgg
    27361 tgacgttagt cgccgtcggg aaggcacaaa agccattgag taccacattg attgtctgcc
    27421 tgaaataaca cgaaaggcac tgcgtgaacg ctatgtggaa cagctggtgg ctacagagaa
    27481 caatgtttct gaagtgaaag ctgttaccag aaaaacacgc aatcctgacg ctgtccaggc
    27541 aatcgaagca tatcgcggtt ctccacaact gatggaagaa cgcctgaatg cgctgaccga
    27601 aaaccagcgc tgggtatctg aagcaagagc tgcgctggtg gtggaagtgc tgaagctgga
    27661 aagcgccggt aaccccgggc gactgaaagc cattaacttt cttgttgaaa aagcccgtaa
    27721 aggtgagctg ccggagcgcc tgcaacaggc cgcagttaac gccaatgcaa aacgtggcgc
    27781 taatcgtact atcagccgcg atccacttta ccagtgggtt ctgaaataca accagtcgca
    27841 gaacgccgcc gaacggctgc tgttactggc accgggaaaa cgcgacgaaa taaaaccgga
    27901 agaaatcagc tggctgcctg aatttctggc gcagtatcgc caggtgaacg gcagacccat
    27961 gtcagaagcc tacgaggatt ttgtcgcaga atggcagcgt cgccatgcgg acgaaccgta
    28021 catgctggag gtgatgcctt cctatgatgt tgtccggtat gccatgaaaa aactgccgga
    28081 agtggtgaaa caaaaagggc gcgttaccgg cagtgaatac cgtcagcttg aaggattcac
    28141 ccgccgcgac tggacagcta tgccggtgaa ttatgtctgg attggtgacg gtcacggtat
    28201 gaagctgaaa tgtgcgcatc cgatccacgg gcgtccattc tcaccggaag tcacgtttgt
    28261 gatcgatggt ggaacacggt ttgttgttgg ctggagtctt gaccttgccg aaaacgtgtt
    28321 cgccgttgcc ggtgcgatac agcacggcat tcgcaaccac ggaaaaccgt tcctgtatta
    28381 ctcggataac ggttccggtg aaacggctga catgctggac aaagaggtcg tgggtattct
    28441 gccacgcctg gggattaaac accctaccgg tattgccggt aatccacagg gacgcggcat
    28501 tattgaacgc ctgaaccgga cactgccgat gcgtatagcc aggaggtatc gcacctattt
    28561 tggcaagggt gctgaccggg aatcgttgcg tgtacttaac cgtgatctgc gttcagcgtt
    28621 taacgccctg caacaggaca agcctctgaa cgaccgccag aaagcggcaa tgcgcgagct
    28681 gccatcgtgg gcggagctta ttgaggctat tcgtgaaggc gtggaatggt acaacaaccg
    28741 cccgcattct gagctgccga tgaaaccgaa cggacggcat tacagcccga cagagttcag
    28801 aaaaaaacgc caggcagaag aagacaccga aattgagtgg ctgagtgatc ttgagctgcg
    28861 cgacatgttc cgcccaatgg tggaacgacc ggtaagacgc tgcgaaattc agtggctgaa
    28921 caatatttac tacgcgccgg agttgcgtga cgaacacggt cgcaaagtgc ttatcagtta
    28981 tgacattcat gacgccgagc gaattacggt acgtcgcaag gacggcagct ttatctgtga
    29041 ggcgatatgg aacggcaata aacgcgcagc ctttgctgtc agtgctgaat atcacaaaca
    29101 acagcagcgt attaaaggaa tgcgcaaacg cgctgaggaa aaaattcgtg atgctgaaga
    29161 tgaaggtatt cagattctgg aacacaaaca ggctgaaccc tggctgagta atgtttatcg
    29221 cccggttggt aatgtggtgg cagttcagca accggaatat gaagaagagc atgacgaaga
    29281 atttgaacgc gatttccggc tggggatgca aaaacttttt gccatgcagg aagaagatga
    29341 tccgctggcc tgaataaaaa aacagtccgc gggaacggac tgttaaataa aggaaacaaa
    29401 gctgatttga aataaccgag gaaatactat gactgatatt gatgatg
//
LOCUS       DAJVPT010000043        29267 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVPT010000043 DAJVPT010000000
VERSION     DAJVPT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..431)
                     /locus_tag="OQM47_001885"
                     /pseudo
     CDS             complement(1..431)
                     /locus_tag="OQM47_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(406..1206)
                     /gene="otsB"
                     /locus_tag="OQM47_001886"
     CDS             complement(406..1206)
                     /gene="otsB"
                     /locus_tag="OQM47_001886"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1480065.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1373..2359)
                     /gene="araH"
                     /locus_tag="OQM47_001887"
     CDS             complement(1373..2359)
                     /gene="araH"
                     /locus_tag="OQM47_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1480066.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2374..3888)
                     /gene="araG"
                     /locus_tag="OQM47_001888"
     CDS             complement(2374..3888)
                     /gene="araG"
                     /locus_tag="OQM47_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1480067.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3958..4947)
                     /gene="araF"
                     /locus_tag="OQM47_001889"
                     /pseudo
     CDS             complement(3958..4947)
                     /gene="araF"
                     /locus_tag="OQM47_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5744..6247
                     /locus_tag="OQM47_001890"
     CDS             5744..6247
                     /locus_tag="OQM47_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1480068.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6327..6578)
                     /locus_tag="OQM47_001891"
     CDS             complement(6327..6578)
                     /locus_tag="OQM47_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1480069.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6693..6779)
                     /gene="azuC"
                     /locus_tag="OQM47_001892"
     CDS             complement(6693..6779)
                     /gene="azuC"
                     /locus_tag="OQM47_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1480070.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7042..7365
                     /locus_tag="OQM47_001893"
     CDS             7042..7365
                     /locus_tag="OQM47_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1480071.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7536..8033
                     /gene="ftnA"
                     /locus_tag="OQM47_001894"
     CDS             7536..8033
                     /gene="ftnA"
                     /locus_tag="OQM47_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1480072.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8070..8309)
                     /locus_tag="OQM47_001895"
     CDS             complement(8070..8309)
                     /locus_tag="OQM47_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1480073.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8499..9710
                     /gene="tyrP"
                     /locus_tag="OQM47_001896"
     CDS             8499..9710
                     /gene="tyrP"
                     /locus_tag="OQM47_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1480074.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9772..10437)
                     /gene="yecA"
                     /locus_tag="OQM47_001897"
     CDS             complement(9772..10437)
                     /gene="yecA"
                     /locus_tag="OQM47_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1480075.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10633..10719)
                     /locus_tag="OQM47_001898"
     tRNA            complement(10633..10719)
                     /locus_tag="OQM47_001898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10683..10685),aa:Leu,seq:taa)
     gene            complement(10732..10805)
                     /locus_tag="OQM47_001899"
     tRNA            complement(10732..10805)
                     /locus_tag="OQM47_001899"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10771..10773),aa:Cys,seq:gca)
     gene            complement(10860..10935)
                     /locus_tag="OQM47_001900"
     tRNA            complement(10860..10935)
                     /locus_tag="OQM47_001900"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10900..10902),aa:Gly,seq:gcc)
     gene            complement(11087..11635)
                     /gene="pgsA"
                     /locus_tag="OQM47_001901"
     CDS             complement(11087..11635)
                     /gene="pgsA"
                     /locus_tag="OQM47_001901"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1480076.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11692..13524)
                     /gene="uvrC"
                     /locus_tag="OQM47_001902"
     CDS             complement(11692..13524)
                     /gene="uvrC"
                     /locus_tag="OQM47_001902"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1480077.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13521..14177)
                     /gene="uvrY"
                     /locus_tag="OQM47_001903"
     CDS             complement(13521..14177)
                     /gene="uvrY"
                     /locus_tag="OQM47_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1480078.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14473..14649
                     /locus_tag="OQM47_001904"
     CDS             14473..14649
                     /locus_tag="OQM47_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480079.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14636..14860
                     /locus_tag="OQM47_001905"
     CDS             14636..14860
                     /locus_tag="OQM47_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1480080.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14893..15513)
                     /gene="sdiA"
                     /locus_tag="OQM47_001906"
                     /pseudo
     CDS             complement(14893..15513)
                     /gene="sdiA"
                     /locus_tag="OQM47_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15743..16495)
                     /gene="yecC"
                     /locus_tag="OQM47_001907"
     CDS             complement(15743..16495)
                     /gene="yecC"
                     /locus_tag="OQM47_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1480081.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16492..17160)
                     /gene="tcyL"
                     /locus_tag="OQM47_001908"
     CDS             complement(16492..17160)
                     /gene="tcyL"
                     /locus_tag="OQM47_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1480082.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17175..18161)
                     /gene="dcyD"
                     /locus_tag="OQM47_001909"
     CDS             complement(17175..18161)
                     /gene="dcyD"
                     /locus_tag="OQM47_001909"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1480083.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18266..19066)
                     /gene="tcyJ"
                     /locus_tag="OQM47_001910"
     CDS             complement(18266..19066)
                     /gene="tcyJ"
                     /locus_tag="OQM47_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1480084.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19154..19705)
                     /gene="fliZ"
                     /locus_tag="OQM47_001911"
     CDS             complement(19154..19705)
                     /gene="fliZ"
                     /locus_tag="OQM47_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1480085.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19751..20470)
                     /gene="fliA"
                     /locus_tag="OQM47_001912"
                     /pseudo
     CDS             complement(19751..20470)
                     /gene="fliA"
                     /locus_tag="OQM47_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20635..22287)
                     /gene="fliC"
                     /locus_tag="OQM47_001913"
     CDS             complement(20635..22287)
                     /gene="fliC"
                     /locus_tag="OQM47_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1480086.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22553..23959
                     /gene="fliD"
                     /locus_tag="OQM47_001914"
     CDS             22553..23959
                     /gene="fliD"
                     /locus_tag="OQM47_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1480087.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23984..24394
                     /gene="fliS"
                     /locus_tag="OQM47_001915"
     CDS             23984..24394
                     /gene="fliS"
                     /locus_tag="OQM47_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1480088.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24394..24759
                     /gene="fliT"
                     /locus_tag="OQM47_001916"
     CDS             24394..24759
                     /gene="fliT"
                     /locus_tag="OQM47_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1480089.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24837..26324
                     /gene="amyA"
                     /locus_tag="OQM47_001917"
     CDS             24837..26324
                     /gene="amyA"
                     /locus_tag="OQM47_001917"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1480090.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26358..26771)
                     /locus_tag="OQM47_001918"
     CDS             complement(26358..26771)
                     /locus_tag="OQM47_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1480091.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26958..28163
                     /gene="yedE"
                     /locus_tag="OQM47_001919"
     CDS             26958..28163
                     /gene="yedE"
                     /locus_tag="OQM47_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1480092.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28160..28393
                     /gene="yedF"
                     /locus_tag="OQM47_001920"
     CDS             28160..28393
                     /gene="yedF"
                     /locus_tag="OQM47_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1480093.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28500..29170
                     /locus_tag="OQM47_001921"
                     /pseudo
     CDS             28500..29170
                     /locus_tag="OQM47_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7290 a         7549 c         6970 g         7458 t
ORIGIN      
        1 attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat cgtggatcca
       61 gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg catttacgcg
      121 tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat aatgaaaagc
      181 gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct gttcgctgag
      241 gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt ccctgtttca
      301 ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat gccaacggca
      361 aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga tactacgact
      421 aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg cggcggttat
      481 catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg cctgagttgc
      541 acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga agccagattc
      601 atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag cttcctgcat
      661 aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga caacacactt
      721 tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta atgtcattaa
      781 tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc ctttcgcctc
      841 cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa tatcacgcgc
      901 aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc gctccgcccc
      961 atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct ccaccattga
     1021 gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct gtagtccttg
     1081 cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg ccagcgttcc
     1141 atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg ttaacggttc
     1201 tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc gctttacaga
     1261 catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca gccaaccgtt
     1321 gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag catcagacag
     1381 tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg attaagccgc
     1441 gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt tccacggtgc
     1501 ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct ttcagagaaa
     1561 cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac gtcattggct
     1621 gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc aggccagaga
     1681 gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt
     1741 cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc agcaaaccaa
     1801 agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac cagttagcgt
     1861 aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct gaaatgatgt
     1921 acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca tttattttca
     1981 gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac aaacccgctg
     2041 ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg acacccgcac
     2101 aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc
     2161 cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata ttaatgaagg
     2221 tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac accaccagca
     2281 tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg
     2341 atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc tgactttagg
     2401 catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt caccggcgat
     2461 ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag gtaagtcgct
     2521 ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt tatatatttc
     2581 gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc
     2641 ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga gattcatgat
     2701 cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat cggcattgtt
     2761 ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc tggcactgat
     2821 gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc gagcttccgg
     2881 gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga tcggctgttg
     2941 gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt tcattaattc
     3001 gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc gaaccgccag
     3061 acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc gctcctcgcc
     3121 ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac
     3181 cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac gtccatcttt
     3241 aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag aaacgtataa
     3301 gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc cgatttcgcg
     3361 ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc
     3421 tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg gcgtgtccgg
     3481 gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca gcaatgagcg
     3541 attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg cgacggtcat
     3601 ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt
     3661 cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg ttggcgcata
     3721 gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac ccatcaacgc
     3781 atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa cgccgggaaa
     3841 cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa cgtctccgtg
     3901 aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc cggcaaatta
     3961 cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg tgatcagtac
     4021 cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc agttgtaaag
     4081 catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac cgtagaagcc
     4141 ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac caataccgat
     4201 gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa gtacggtgct
     4261 gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca gcattgagtt
     4321 ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat aaatttgttt
     4381 ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt
     4441 atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc agccacgttt
     4501 ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg ccgccatcat
     4561 caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt catccactgc
     4621 aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc
     4681 cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa
     4741 tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat ccttcccggc
     4801 tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct tcaccagaaa
     4861 accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca gaccaatggc
     4921 tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag
     4981 ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc ttttatctaa
     5041 aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat aggagagcaa
     5101 tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa gacataagga
     5161 aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata agattagcct
     5221 ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc agtcaatagg
     5281 ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca aagatgtgga
     5341 aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta acagaacacg
     5401 tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc acttcatcat
     5461 gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat taaaattcat
     5521 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     5581 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     5641 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     5701 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     5761 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     5821 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     5881 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     5941 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     6001 gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc agttagccga
     6061 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     6121 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     6181 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     6241 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     6301 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     6361 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     6421 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     6481 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     6541 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     6601 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     6661 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     6721 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     6781 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     6841 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     6901 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     6961 cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     7021 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     7081 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     7141 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
     7201 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     7261 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     7321 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     7381 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     7441 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     7501 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     7561 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     7621 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     7681 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     7741 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     7801 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     7861 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     7921 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     7981 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     8041 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     8101 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     8161 cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     8221 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     8281 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     8341 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     8401 atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
     8461 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     8521 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     8581 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     8641 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     8701 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     8761 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     8821 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     8881 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     8941 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     9001 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     9061 cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     9121 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     9181 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     9241 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct
     9301 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     9361 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     9421 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     9481 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     9541 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     9601 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     9661 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     9721 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     9781 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     9841 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     9901 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     9961 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
    10021 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
    10081 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
    10141 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
    10201 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
    10261 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
    10321 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
    10381 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
    10441 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
    10501 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
    10561 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
    10621 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
    10681 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
    10741 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
    10801 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
    10861 ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt gctctaccaa
    10921 ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
    10981 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
    11041 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
    11101 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
    11161 caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac agcagccagg
    11221 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
    11281 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
    11341 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
    11401 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
    11461 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
    11521 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
    11581 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
    11641 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
    11701 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
    11761 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
    11821 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
    11881 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
    11941 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
    12001 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
    12061 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
    12121 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
    12181 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
    12241 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
    12301 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
    12361 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
    12421 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
    12481 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
    12541 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
    12601 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
    12661 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
    12721 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
    12781 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
    12841 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
    12901 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
    12961 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
    13021 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
    13081 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
    13141 atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc
    13201 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
    13261 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
    13321 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
    13381 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
    13441 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
    13501 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
    13561 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
    13621 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
    13681 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
    13741 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    13801 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    13861 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    13921 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    13981 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    14041 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    14101 cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    14161 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    14221 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    14281 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    14341 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    14401 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    14461 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    14521 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    14581 gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa aataaatgag
    14641 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    14701 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    14761 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    14821 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    14881 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    14941 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    15001 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    15061 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    15121 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    15181 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    15241 gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    15301 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    15361 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    15421 ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    15481 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    15541 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    15601 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    15661 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    15721 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggtgc
    15781 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    15841 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    15901 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    15961 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    16021 gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg
    16081 tttctttacc ggccagccca actttcgcca gcaactcgcg agcgcgcgcc gtggcctctt
    16141 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    16201 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    16261 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    16321 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    16381 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    16441 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    16501 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    16561 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    16621 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    16681 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    16741 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    16801 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    16861 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    16921 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    16981 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    17041 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    17101 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    17161 gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    17221 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    17281 cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag acgcgccagc
    17341 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    17401 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    17461 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    17521 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    17581 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    17641 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    17701 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg ggcattggga
    17761 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    17821 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    17881 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    17941 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    18001 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    18061 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    18121 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    18181 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    18241 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    18301 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    18361 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    18421 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    18481 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    18541 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    18601 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    18661 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    18721 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    18781 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    18841 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    18901 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc tctttgactt
    18961 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgg
    19021 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    19081 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    19141 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    19201 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    19261 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    19321 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    19381 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    19441 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    19501 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    19561 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    19621 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    19681 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    19741 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc tggctgtgta
    19801 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    19861 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    19921 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    19981 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    20041 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    20101 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    20161 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    20221 gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta aatgccgttc
    20281 cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    20341 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    20401 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    20461 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    20521 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    20581 aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag acgattaacc
    20641 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    20701 ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    20761 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    20821 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    20881 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    20941 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    21001 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    21061 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctgc
    21121 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    21181 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    21241 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    21301 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    21361 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    21421 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    21481 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    21541 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    21601 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    21661 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    21721 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    21781 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    21841 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    21901 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    21961 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    22021 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    22081 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    22141 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    22201 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    22261 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    22321 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    22381 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    22441 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    22501 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    22561 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    22621 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    22681 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    22741 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    22801 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    22861 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    22921 cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta ccattgatat
    22981 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    23041 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    23101 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    23161 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    23221 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    23281 cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    23341 catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga atgcctataa
    23401 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    23461 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    23521 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    23581 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    23641 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    23701 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    23761 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    23821 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    23881 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    23941 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    24001 cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    24061 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    24121 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    24181 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    24241 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    24301 agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    24361 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    24421 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    24481 tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    24541 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    24601 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    24661 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    24721 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    24781 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    24841 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    24901 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    24961 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    25021 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    25081 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    25141 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    25201 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    25261 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    25321 gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt aacgactata
    25381 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    25441 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    25501 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    25561 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    25621 ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    25681 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    25741 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    25801 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    25861 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    25921 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    25981 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    26041 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    26101 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    26161 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    26221 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    26281 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    26341 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    26401 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    26461 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    26521 gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg gcaatccaag
    26581 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    26641 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    26701 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    26761 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    26821 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    26881 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    26941 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    27001 gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    27061 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    27121 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    27181 cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgctgctg
    27241 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    27301 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    27361 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    27421 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    27481 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    27541 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    27601 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    27661 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    27721 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    27781 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    27841 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    27901 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    27961 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    28021 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    28081 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gaggcaccgc
    28141 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    28201 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    28261 tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    28321 gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca ttcgttattt
    28381 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    28441 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    28501 tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    28561 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    28621 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    28681 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    28741 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    28801 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    28861 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    28921 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    28981 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    29041 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    29101 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    29161 tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt caggctgtcg
    29221 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgc
//
LOCUS       DAJVPT010000044        29115 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVPT010000044 DAJVPT010000000
VERSION     DAJVPT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            61..342
                     /locus_tag="OQM47_001922"
     CDS             61..342
                     /locus_tag="OQM47_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1480094.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            308..1385
                     /locus_tag="OQM47_001923"
                     /pseudo
     CDS             308..1385
                     /locus_tag="OQM47_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1778..2119)
                     /locus_tag="OQM47_001924"
     CDS             complement(1778..2119)
                     /locus_tag="OQM47_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1480095.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2132..3004)
                     /gene="copD"
                     /locus_tag="OQM47_001925"
                     /pseudo
     CDS             complement(2132..3004)
                     /gene="copD"
                     /locus_tag="OQM47_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3008..3382)
                     /gene="yobA"
                     /locus_tag="OQM47_001926"
     CDS             complement(3008..3382)
                     /gene="yobA"
                     /locus_tag="OQM47_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1480096.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3521..3751
                     /gene="holE"
                     /locus_tag="OQM47_001927"
     CDS             3521..3751
                     /gene="holE"
                     /locus_tag="OQM47_001927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1480097.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3853..4509
                     /locus_tag="OQM47_001928"
                     /pseudo
     CDS             3853..4509
                     /locus_tag="OQM47_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4533..5195
                     /gene="exoX"
                     /locus_tag="OQM47_001929"
     CDS             4533..5195
                     /gene="exoX"
                     /locus_tag="OQM47_001929"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1480098.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5192..7252)
                     /gene="ptrB"
                     /locus_tag="OQM47_001930"
     CDS             complement(5192..7252)
                     /gene="ptrB"
                     /locus_tag="OQM47_001930"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1480099.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7462..8121)
                     /locus_tag="OQM47_001931"
     CDS             complement(7462..8121)
                     /locus_tag="OQM47_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1480100.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8448..8804)
                     /locus_tag="OQM47_001932"
     CDS             complement(8448..8804)
                     /locus_tag="OQM47_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1480101.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OQM47_001933"
                     /pseudo
     CDS             complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OQM47_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9295..10473
                     /gene="purT"
                     /locus_tag="OQM47_001934"
     CDS             9295..10473
                     /gene="purT"
                     /locus_tag="OQM47_001934"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1480102.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10529..11170)
                     /gene="kdgA"
                     /locus_tag="OQM47_001935"
                     /gene_synonym="eda"
     CDS             complement(10529..11170)
                     /gene="kdgA"
                     /locus_tag="OQM47_001935"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1480103.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11207..13018)
                     /gene="edd"
                     /locus_tag="OQM47_001936"
     CDS             complement(11207..13018)
                     /gene="edd"
                     /locus_tag="OQM47_001936"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1480104.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13253..14728)
                     /gene="zwf"
                     /locus_tag="OQM47_001937"
     CDS             complement(13253..14728)
                     /gene="zwf"
                     /locus_tag="OQM47_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1480105.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15066..15935
                     /gene="hexR"
                     /locus_tag="OQM47_001938"
                     /gene_synonym="yebK"
     CDS             15066..15935
                     /gene="hexR"
                     /locus_tag="OQM47_001938"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1480106.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16063..17505
                     /gene="pyk"
                     /locus_tag="OQM47_001939"
     CDS             16063..17505
                     /gene="pyk"
                     /locus_tag="OQM47_001939"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1480107.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17636..18607)
                     /gene="lpxM"
                     /locus_tag="OQM47_001940"
                     /gene_synonym="msbB"
     CDS             complement(17636..18607)
                     /gene="lpxM"
                     /locus_tag="OQM47_001940"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1480108.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18727..20049)
                     /gene="mepM"
                     /locus_tag="OQM47_001941"
     CDS             complement(18727..20049)
                     /gene="mepM"
                     /locus_tag="OQM47_001941"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1480109.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20065..20997)
                     /gene="znuA"
                     /locus_tag="OQM47_001942"
     CDS             complement(20065..20997)
                     /gene="znuA"
                     /locus_tag="OQM47_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1480110.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21076..21831
                     /gene="znuC"
                     /locus_tag="OQM47_001943"
     CDS             21076..21831
                     /gene="znuC"
                     /locus_tag="OQM47_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1480111.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21828..22613
                     /gene="znuB"
                     /locus_tag="OQM47_001944"
     CDS             21828..22613
                     /gene="znuB"
                     /locus_tag="OQM47_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1480112.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22760..23770)
                     /gene="ruvB"
                     /locus_tag="OQM47_001945"
     CDS             complement(22760..23770)
                     /gene="ruvB"
                     /locus_tag="OQM47_001945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1480113.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23779..24390)
                     /gene="ruvA"
                     /locus_tag="OQM47_001946"
     CDS             complement(23779..24390)
                     /gene="ruvA"
                     /locus_tag="OQM47_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1480114.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24529..24594)
                     /gene="yobI"
                     /locus_tag="OQM47_001947"
     CDS             complement(24529..24594)
                     /gene="yobI"
                     /locus_tag="OQM47_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1480115.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24664..25266
                     /locus_tag="OQM47_001948"
     CDS             24664..25266
                     /locus_tag="OQM47_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1480116.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25268..25789)
                     /gene="ruvC"
                     /locus_tag="OQM47_001949"
     CDS             complement(25268..25789)
                     /gene="ruvC"
                     /locus_tag="OQM47_001949"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1480117.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25824..26564)
                     /locus_tag="OQM47_001950"
     CDS             complement(25824..26564)
                     /locus_tag="OQM47_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1480118.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26593..27045)
                     /gene="nudB"
                     /locus_tag="OQM47_001951"
     CDS             complement(26593..27045)
                     /gene="nudB"
                     /locus_tag="OQM47_001951"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1480119.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27163..28935)
                     /gene="aspS"
                     /locus_tag="OQM47_001952"
     CDS             complement(27163..28935)
                     /gene="aspS"
                     /locus_tag="OQM47_001952"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1480120.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
BASE COUNT         7137 a         7491 c         7148 g         7339 t
ORIGIN      
        1 caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag aggtatggca
       61 atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa cccaccatca
      121 atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc tgaactacac
      181 gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa ggttgatacc
      241 gatataacac accatcagcc taatgggcga caagggagag acagtccgtt aatggagaag
      301 ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa catatcgaag
      361 gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta cactaggtaa
      421 aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata tatacaaaaa
      481 ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta ctatggcagg
      541 ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa atacttataa
      601 aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt tactgacaga
      661 aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag ggaaaaacac
      721 gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca ttgttgaagg
      781 acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag ttacaagaga
      841 acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac ttccggcatg
      901 gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag acataacgaa
      961 tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta agacaggaat
     1021 gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac ttggtgcagt
     1081 agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg ggattagaaa
     1141 aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg tcgcagccag
     1201 aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa atcagaagcc
     1261 tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt ctacttggcc
     1321 acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg tacttactgc
     1381 tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa ccaaaaaaga
     1441 ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt gcaactaatt
     1501 gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc cagggaaatg
     1561 gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag ttgccttgcc
     1621 ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg tcaataaaaa
     1681 gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag aactgaggcg
     1741 gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt tcagctcgtt
     1801 ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca atctgaatca
     1861 ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg atatcgttta
     1921 aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc ggtcggcaag
     1981 tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca aaacgactca
     2041 cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac agaaacgcca
     2101 gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc gcaagcaggc
     2161 tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc cacacaaacc
     2221 agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa cgattagcca
     2281 gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt tggccccagt
     2341 aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg ccgcttgcca
     2401 gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc tgaatagcct
     2461 agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt aatcccccaa
     2521 accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt ttcgctgtta
     2581 ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc gcggtagtga
     2641 gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc aacgttacga
     2701 gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa acagcctgcc
     2761 aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc tgaacggcga
     2821 gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa cgcagagaac
     2881 gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac attgcaaagc
     2941 caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag gtaaacgcca
     3001 gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg tccaccgaaa
     3061 caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt ggaacaatca
     3121 gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt tcgttttttg
     3181 gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag tttaaagtga
     3241 ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc gttaaatgcg
     3301 catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc gcgtagcgaa
     3361 gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt tttacagagc
     3421 ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat ccaggctcac
     3481 tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga atctggctaa
     3541 actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg gggtggcttt
     3601 taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac agccagaaca
     3661 tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg tcaatctgtc
     3721 acgtttacct tacgagccca aacttaaata aaacttatac agagttacac tttcttacat
     3781 aacgcctgct aaattatgag tgttttctaa accgccccca taatttgcag tcattttgaa
     3841 aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc tcgtaaaacc
     3901 tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc caggcaacaa
     3961 tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc gcgacgtgcc
     4021 cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc aacgacctag
     4081 cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat tcgtggaatt
     4141 gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca tggtgcatgt
     4201 ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg tatggatatg
     4261 gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga cctgctggcg
     4321 tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct gatggcaaat
     4381 gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg cgccgatcgt
     4441 ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga tatcattcca
     4501 ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat acagaaacct
     4561 gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac ggaaaaatcg
     4621 tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa gcgatggcga
     4681 ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat gtgatcccac
     4741 actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc cgcgtactgc
     4801 ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg tggccagggc
     4861 tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag acaccgctgg
     4921 gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg cttatcgata
     4981 ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga cgtccgtcgt
     5041 tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac gttgccgaac
     5101 gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc ccggagctgc
     5161 gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg ggtaatgttc
     5221 cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac gatttaaagc
     5281 ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa agatggtcat
     5341 cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat tgcacctgag
     5401 aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg acgttgtcat
     5461 acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt ccccactctt
     5521 caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca acatcaacaa
     5581 acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt gcaacgccca
     5641 tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag ccatagccca
     5701 gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc ttcttcagaa
     5761 atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca tggacaatgg
     5821 cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg gcatcaatac
     5881 ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg cgaaaatgtt
     5941 tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact atccacaggt
     6001 gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt aatacacgac
     6061 gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg gaagaataac
     6121 cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc acataggccg
     6181 gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg cgcaaactgg
     6241 ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta aacccttcca
     6301 gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca cgcatacggg
     6361 tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa cgatgctggt
     6421 agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac ggctcggcat
     6481 cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc agatgaatga
     6541 cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg tctttttctt
     6601 cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag acctgataag
     6661 gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca tttgcccaga
     6721 caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt tccagattac
     6781 gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc ataatggtgt
     6841 tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct gcgcgcttgt
     6901 tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat tgacgctggt
     6961 agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca ttttttatgt
     7021 agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag atgcgatcct
     7081 gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt tgcaggtagt
     7141 ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta tcgatgcgcg
     7201 tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc atgttattgt
     7261 tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac
     7321 agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac
     7381 agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa tagtcaactt
     7441 caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg
     7501 ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta
     7561 actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag
     7621 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
     7681 tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc
     7741 gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa agaccagcgc
     7801 aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
     7861 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
     7921 cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga
     7981 tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta acagtttgcc
     8041 caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag
     8101 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
     8161 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg
     8221 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat caggtgataa
     8281 gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc
     8341 gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg gacggacaag
     8401 gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
     8461 tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc ggcactttta
     8521 ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg
     8581 acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca gcgcgccacg
     8641 cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca
     8701 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
     8761 gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
     8821 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
     8881 tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg
     8941 gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga aagggcttca
     9001 cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag cagatccgcc
     9061 gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct
     9121 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
     9181 ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca
     9241 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
     9301 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
     9361 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
     9421 cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
     9481 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
     9541 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
     9601 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
     9661 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag ccttttccgc
     9721 gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc
     9781 aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga atacgctcag
     9841 caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
     9901 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accagtaggt
     9961 catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt
    10021 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt
    10081 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct
    10141 cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga gtttgccctg
    10201 catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg tcctgcagct
    10261 tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
    10321 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
    10381 catctggggg tggtactggc tacttcagag agtgttgttg acgccattga acgcgcgaag
    10441 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc tacaaaaatg
    10501 cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca gcttcacgcg
    10561 ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga accagccagg
    10621 aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta gccggagaaa
    10681 taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt
    10741 tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg tagtccatac
    10801 ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc ccttcggtag
    10861 cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca cccgcttcag
    10921 tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca
    10981 cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca
    11041 gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt tccagttttt
    11101 ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc
    11161 agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac
    11221 aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc
    11281 ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg
    11341 cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt
    11401 cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg atagctgacg
    11461 gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc
    11521 ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt
    11581 ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg
    11641 ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct
    11701 caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt aacacttttg
    11761 tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg tcgagtgatt
    11821 tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca agggtataac
    11881 gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca
    11941 gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac
    12001 cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc
    12061 agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg
    12121 aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct
    12181 taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga cgcgcagcgg
    12241 cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact
    12301 gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag aaagtacatg
    12361 ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg tccactttac
    12421 cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg
    12481 gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc atcgtcagac
    12541 ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg ttatgggaca
    12601 gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat tccattccat
    12661 cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg
    12721 cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa ggctggtgcg
    12781 cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca
    12841 aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca
    12901 actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga taagcagagc
    12961 gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt ggattcataa
    13021 aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca ttcagaggat
    13081 ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat
    13141 aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat
    13201 ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga tattactcaa
    13261 actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg ggtccccagg
    13321 ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt
    13381 ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc tgaataccac
    13441 gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga ttaaaggttt
    13501 ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa
    13561 ctttattcag tacctggata tccacgcctt catcaggttg cagacggata gtcagtttat
    13621 tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga
    13681 ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac
    13741 cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc
    13801 ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg gcgaagcccg
    13861 cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg cggcgcaggg
    13921 acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gatggcggag
    13981 acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca cgcatctgac
    14041 cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct
    14101 caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta gcaaaacgca
    14161 gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg taaacctggc
    14221 actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc agcgacgtcc
    14281 ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc
    14341 ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata gtgatacgat
    14401 ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg acatcgagat
    14461 tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca
    14521 tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc cagtcagcac
    14581 gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc agttgataca
    14641 gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac
    14701 aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact
    14761 taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg
    14821 aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa gcgcggcaaa
    14881 actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc atgttatgaa
    14941 aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg cgtaatatct
    15001 gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt gagtctcttt
    15061 acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc aaatcagagc
    15121 gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg agtattgctg
    15181 ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg
    15241 acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg aatggcactc
    15301 cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg gggaaaatat
    15361 ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa tctgccatca
    15421 accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt
    15481 ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt ccggtggtgt
    15541 actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg
    15601 tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg cagctggcac
    15661 gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc gcccgggaag
    15721 caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg cccatggttt
    15781 ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact ttgcgacgcg
    15841 gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg
    15901 ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg tcctaatctt
    15961 atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat tcggatttca
    16021 tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa aaggcttcgc
    16081 agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa tcttgaaaaa
    16141 gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc gcctgaagat
    16201 cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg tcatgtggct
    16261 attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt
    16321 ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg tgaaggcgac
    16381 aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc tggtgacatc
    16441 ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg catgaaagtg
    16501 ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa caaacttggc
    16561 ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa gactgcggcg
    16621 ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga tctgaactat
    16681 gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt
    16741 gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc tgacgtggta
    16801 atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt cggcattcag
    16861 aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc gacccagatg
    16921 atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac
    16981 gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc tgggcagtat
    17041 ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc
    17101 atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga agctattgcc
    17161 atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat taccatgacc
    17221 gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctgcc aattttcgcc
    17281 atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt tacgccggtg
    17341 ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa tctgctgcgc
    17401 gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga cgtgatgagt
    17461 accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg ttgccggatg
    17521 cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt tcgaatattc
    17581 tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg cttttttatt
    17641 tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg cgagttttca
    17701 gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc tcgacttctt
    17761 cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc atcggtgggc
    17821 gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc ggtacaacgc
    17881 gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa
    17941 agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa taatatcccc
    18001 agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc gcatgcagac
    18061 gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg ttgccctgat
    18121 tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca ggaatatcga
    18181 cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc atctcttcga
    18241 tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg cgtattgcca
    18301 actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca acaatcgctt
    18361 cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac gcacgacggc
    18421 gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg ggatcacgga
    18481 actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat gctccccagt
    18541 agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta ttttttttcg
    18601 tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag tgtagcggca
    18661 caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc tgcgaatgga
    18721 tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca ggaattcgcg
    18781 acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg ggtttacggc
    18841 ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc cggtattacc
    18901 ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca gaatcttgcg
    18961 caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat aataacctgc
    19021 tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca ctggcgtacc
    19081 ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca ccggattagt
    19141 tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga atcgcaagaa
    19201 tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag cgcggattgc
    19261 gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt gctcacgttt
    19321 accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt tcagtttgcg
    19381 gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc tggttaaacc
    19441 ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca gattgttaac
    19501 ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag tacggtcata
    19561 ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt
    19621 taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt cggcagcagc
    19681 aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc ttagcgtatc
    19741 gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt catccagttc
    19801 gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac tggcttcagg
    19861 taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg gcgtggcatc
    19921 acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg tgagcgaccc
    19981 caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag agcgggctat
    20041 ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat actggttggc
    20101 taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg tccccagggg
    20161 atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct
    20221 gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg ttcttatttc
    20281 atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac caagcggtgt
    20341 cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat aacctttccc
    20401 cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt gtgcctcaaa
    20461 atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca ctaattttcc
    20521 atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat gcatgttgaa
    20581 atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc
    20641 gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg ttacctgctt
    20701 cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg ggccaaccca
    20761 aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg aataatcatg
    20821 ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag caatggcaga
    20881 agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg cctgtgttgc
    20941 acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat gtaacataat
    21001 gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca tgactactgc
    21061 aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt ttcttttggc
    21121 caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta
    21181 cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg gctggtaaca
    21241 cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt accgcagaag
    21301 ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt acgccctggt
    21361 acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca cctgattaac
    21421 gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc gcgagcattg
    21481 ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga tgtaaatggc
    21541 caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg tggcgtttta
    21601 atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct gtgcctgaat
    21661 caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga gtttatttca
    21721 atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca taatcatcgt
    21781 cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg attgaattat
    21841 tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt
    21901 ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc tcattacttg
    21961 gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt gccgttacgc
    22021 tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg atcgacacgt
    22081 tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt agtctgatgt
    22141 ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca gtgacgccag
    22201 aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg ttctggcaat
    22261 ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat ggtgtgaaat
    22321 tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt gtagcgatga
    22381 aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct actgcgcgtc
    22441 gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg atggtggcag
    22501 taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg tcggtggtgc
    22561 tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc taatctatcg
    22621 ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt cgctatctct
    22681 ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg tcggatgcgg
    22741 cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag tgattccacg
    22801 cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc tgaatcaaat
    22861 aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc gccaggttat
    22921 ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat ttgcggtcca
    22981 tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca gcgatatctg
    23041 ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc agcagacggt
    23101 tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg tcatcactca
    23161 tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc ggcacctgat
    23221 aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc agcgaacctg
    23281 cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg gagcgtgccg
    23341 ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg tacagcactt
    23401 cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca tgcggttcaa
    23461 ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca gaagtcgtgc
    23521 gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta cccaaccccg
    23581 gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct gctttgatga
    23641 aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt aatttagggc
    23701 gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc agacggtctg
    23761 cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta atgtttcact
    23821 gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg gtttatagcc
    23881 tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg ggctagcagg
    23941 agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac ctttaaatcg
    24001 gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg gcagtttcac
    24061 cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg acattccgga
    24121 gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact ctttgaacaa
    24181 cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac gcaccacaaa
    24241 gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac aggtcatcgg
    24301 catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg gttgtttttc
    24361 aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga atcaaagata
    24421 atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga aaacttgcga
    24481 ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt atattggaag
    24541 caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat acattgcgac
    24601 tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca atggaatgac
    24661 aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt tacatgtata
    24721 ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa tcacgtcggg
    24781 atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt tcccctttca
    24841 accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta tgctataaag
    24901 cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca ggttccctcc
    24961 cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca gttctgttca
    25021 aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga aatagagacg
    25081 tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa attctggttc
    25141 ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg gcatcatccg
    25201 ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact gaccgaggcg
    25261 gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc atctgcatcg
    25321 cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg gcatccgcct
    25381 gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg cttttttcgg
    25441 cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca aataccggca
    25501 attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt ttcagggctg
    25561 agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc tggaactggg
    25621 tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa tcatccactt
    25681 tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg cggatgacgc
    25741 cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc acgcgtctcc
    25801 gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga gatttcaccg
    25861 ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag acgcatcagt
    25921 ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga aacttccgcg
    25981 ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat ttcttcccat
    26041 gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc acctgcttcc
    26101 agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac gccttttttg
    26161 ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt gctgaatgcg
    26221 tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat catgattgcc
    26281 gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc atcatcaccg
    26341 cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt agacagtgct
    26401 ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc cagcttagcc
    26461 gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc atcctgcgca
    26521 gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat atctccagat
    26581 agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc ggttgctcca
    26641 ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca gatgttcagt
    26701 gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg tattacgcgt
    26761 cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta ccgtgcgctg
    26821 acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt cctttacttc
    26881 gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta ccgactgcca
    26941 gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt gtgcatagat
    27001 gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc ctcttcgttg
    27061 actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc actcagttgc
    27121 cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat tctcagcctt
    27181 cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc tcggtgcttc
    27241 agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc
    27301 ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg cgtgcggcgg
    27361 agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt
    27421 gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac gtactgaacc
    27481 accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg cattttccgg
    27541 tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat ggtgcattgc
    27601 cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc acagcggtgc
    27661 ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca gtgcgcccat
    27721 cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc catcttgcgc
    27781 ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg ctaccgggct
    27841 gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca gacctttcgc
    27901 gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca gcgatgcgcc
    27961 acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc ccgcaaatac
    28021 tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg ggttacgcag
    28081 atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta ccgggaaatc
    28141 gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt ccatcacttc
    28201 acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa attcaggctg
    28261 acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt aacggtcaaa
    28321 accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg cgtagaattt
    28381 acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg tggctttggt
    28441 cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc gcaccaggct
    28501 ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca ggtcgaggta
    28561 gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg gcagaacatc
    28621 tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg tcgccatatc
    28681 gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa tgcagaactc
    28741 attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga aaaatacctg
    28801 cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat cacgacgacg
    28861 gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga gctgtccaca
    28921 atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc cctgctgcgc
    28981 aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag gcggctatta
    29041 tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct cgttaccact
    29101 ttatatcggg cctga
//
LOCUS       DAJVPT010000045        28904 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVPT010000045 DAJVPT010000000
VERSION     DAJVPT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..2118)
                     /gene="yccS"
                     /locus_tag="OQM47_001953"
     CDS             complement(<1..2118)
                     /gene="yccS"
                     /locus_tag="OQM47_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1480121.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQ"
     gene            complement(2128..2574)
                     /locus_tag="OQM47_001954"
     CDS             complement(2128..2574)
                     /locus_tag="OQM47_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1480122.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2697..4751
                     /gene="helD"
                     /locus_tag="OQM47_001955"
     CDS             2697..4751
                     /gene="helD"
                     /locus_tag="OQM47_001955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1480123.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4783..5241)
                     /gene="mgsA"
                     /locus_tag="OQM47_001956"
     CDS             complement(4783..5241)
                     /gene="mgsA"
                     /locus_tag="OQM47_001956"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1480124.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5337..5999)
                     /locus_tag="OQM47_001957"
     CDS             complement(5337..5999)
                     /locus_tag="OQM47_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1480125.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6172..6585
                     /locus_tag="OQM47_001958"
     CDS             6172..6585
                     /locus_tag="OQM47_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1480126.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6630..6947)
                     /gene="hspQ"
                     /locus_tag="OQM47_001959"
     CDS             complement(6630..6947)
                     /gene="hspQ"
                     /locus_tag="OQM47_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1480127.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7005..8195)
                     /gene="rlmI"
                     /locus_tag="OQM47_001960"
     CDS             complement(7005..8195)
                     /gene="rlmI"
                     /locus_tag="OQM47_001960"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1480128.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8290..8568
                     /gene="yccX"
                     /locus_tag="OQM47_001961"
     CDS             8290..8568
                     /gene="yccX"
                     /locus_tag="OQM47_001961"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1480129.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8565..8894)
                     /gene="tusE"
                     /locus_tag="OQM47_001962"
     CDS             complement(8565..8894)
                     /gene="tusE"
                     /locus_tag="OQM47_001962"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1480130.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8985..9644)
                     /gene="yccA"
                     /locus_tag="OQM47_001963"
     CDS             complement(8985..9644)
                     /gene="yccA"
                     /locus_tag="OQM47_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1480131.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9851..9938)
                     /locus_tag="OQM47_001964"
     tRNA            complement(9851..9938)
                     /locus_tag="OQM47_001964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9902..9904),aa:Ser,seq:tga)
     gene            10364..11482
                     /gene="hyaA"
                     /locus_tag="OQM47_001965"
     CDS             10364..11482
                     /gene="hyaA"
                     /locus_tag="OQM47_001965"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1480132.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11479..13272
                     /gene="hyaB"
                     /locus_tag="OQM47_001966"
     CDS             11479..13272
                     /gene="hyaB"
                     /locus_tag="OQM47_001966"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1480133.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13291..13998
                     /gene="hyaC"
                     /locus_tag="OQM47_001967"
     CDS             13291..13998
                     /gene="hyaC"
                     /locus_tag="OQM47_001967"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1480134.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13995..14582
                     /locus_tag="OQM47_001968"
     CDS             13995..14582
                     /locus_tag="OQM47_001968"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1480135.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14579..14977
                     /gene="hyaE"
                     /locus_tag="OQM47_001969"
     CDS             14579..14977
                     /gene="hyaE"
                     /locus_tag="OQM47_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1480136.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14974..15831
                     /locus_tag="OQM47_001970"
     CDS             14974..15831
                     /locus_tag="OQM47_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1480137.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15965..17509
                     /gene="appC"
                     /locus_tag="OQM47_001971"
     CDS             15965..17509
                     /gene="appC"
                     /locus_tag="OQM47_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1480138.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17521..18657
                     /gene="appB"
                     /locus_tag="OQM47_001972"
     CDS             17521..18657
                     /gene="appB"
                     /locus_tag="OQM47_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1480139.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18669..18761
                     /gene="cbdX"
                     /locus_tag="OQM47_001973"
     CDS             18669..18761
                     /gene="cbdX"
                     /locus_tag="OQM47_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1480140.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18841..20139
                     /gene="appA"
                     /locus_tag="OQM47_001974"
     CDS             18841..20139
                     /gene="appA"
                     /locus_tag="OQM47_001974"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1480141.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20254..22420)
                     /gene="etk"
                     /locus_tag="OQM47_001975"
                     /pseudo
     CDS             complement(20254..22420)
                     /gene="etk"
                     /locus_tag="OQM47_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22440..22886)
                     /gene="etp"
                     /locus_tag="OQM47_001976"
                     /gene_synonym="yccY"
     CDS             complement(22440..22886)
                     /gene="etp"
                     /locus_tag="OQM47_001976"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1480142.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22874..24013)
                     /locus_tag="OQM47_001977"
     CDS             complement(22874..24013)
                     /locus_tag="OQM47_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1480143.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24059..26155)
                     /locus_tag="OQM47_001978"
     CDS             complement(24059..26155)
                     /locus_tag="OQM47_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1480144.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26155..26901)
                     /locus_tag="OQM47_001979"
     CDS             complement(26155..26901)
                     /locus_tag="OQM47_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1480145.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26898..27542)
                     /locus_tag="OQM47_001980"
     CDS             complement(26898..27542)
                     /locus_tag="OQM47_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1480146.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27649..27954)
                     /locus_tag="OQM47_001981"
     CDS             complement(27649..27954)
                     /locus_tag="OQM47_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480147.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28396..28608)
                     /gene="cspH"
                     /locus_tag="OQM47_001982"
     CDS             complement(28396..28608)
                     /gene="cspH"
                     /locus_tag="OQM47_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1480148.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7001 a         7319 c         7597 g         6987 t
ORIGIN      
        1 ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt gtacgaccag
       61 aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca ggccagctaa
      121 tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca
      181 aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc
      241 gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa acgcggcttc
      301 gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg acgccagttc
      361 agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc
      421 ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg tggcgcgttc
      481 gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca ctgccgccca
      541 cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc
      601 acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta
      661 ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc
      721 ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta ccagcgtacc
      781 aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa
      841 acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga tggcgtagcc
      901 gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa
      961 gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat cagcgagctc
     1021 attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg
     1081 ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg
     1141 tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa
     1201 atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt gctggcacgc
     1261 ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg
     1321 ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc
     1381 gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg
     1441 ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt tggcgagagc
     1501 caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt
     1561 gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg
     1621 gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg caccggccag
     1681 cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat
     1741 ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa
     1801 aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag
     1861 caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa
     1921 acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag
     1981 cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc
     2041 aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg
     2101 acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg
     2161 cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc
     2221 ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat
     2281 gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt
     2341 caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca gttcatcgac
     2401 atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg
     2461 tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag
     2521 agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc
     2581 tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca
     2641 cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg
     2701 aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga
     2761 tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc
     2821 aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa tttgtactgc
     2881 cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag cgtttttacc
     2941 atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg
     3001 ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc
     3061 gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc
     3121 cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggctt
     3181 ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc gaagccatgc
     3241 ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg
     3301 cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca ggaagcggaa
     3361 aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg
     3421 agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac gagcggattc
     3481 gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg gcgctgcata
     3541 ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg
     3601 cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg
     3661 cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact
     3721 tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc
     3781 tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc
     3841 gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg
     3901 cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg attgtgattc
     3961 tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata
     4021 tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt
     4081 tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc
     4141 tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta gacacgactt
     4201 accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag aacccaggcc
     4261 agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc acgttattgg
     4321 atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagagc
     4381 gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa
     4441 cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg
     4501 cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg gctgcggcgc
     4561 gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag
     4621 aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta
     4681 acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga
     4741 gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtcagc
     4801 gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat gcggcgactg
     4861 gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgtcgccag
     4921 acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat cccagaagaa
     4981 tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat
     5041 tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca agttaccggt
     5101 agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct
     5161 catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa
     5221 agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta
     5281 agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag
     5341 gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt
     5401 ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc
     5461 agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt
     5521 agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt
     5581 tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca
     5641 tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg
     5701 cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac caccagcggt
     5761 ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac
     5821 actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag cagagagctg
     5881 gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg
     5941 gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata
     6001 ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata
     6061 cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg
     6121 gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa
     6181 accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac
     6241 aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc
     6301 attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg gtatggcacg
     6361 ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg
     6421 tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat gcaactgggg
     6481 gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt ggtgatggat
     6541 cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat ccccggaagg
     6601 caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc ctggagttgt
     6661 ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat
     6721 tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc
     6781 atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag ctcatcgggc
     6841 gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac
     6901 agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg
     6961 gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag
     7021 gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg atcggctgcc
     7081 tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc atccgcgatg
     7141 attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg
     7201 ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg acacgcgccc
     7261 atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt
     7321 tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc
     7381 ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg
     7441 gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca
     7501 ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg
     7561 cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat atccaccagc
     7621 agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg
     7681 ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc
     7741 gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg atattctgcg
     7801 ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc
     7861 aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc
     7921 cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac
     7981 tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg
     8041 cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta
     8101 ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt
     8161 tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag
     8221 aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg
     8281 agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt tcagggcgta
     8341 ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg gtacgccaaa
     8401 aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtagaaaaa
     8461 ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc
     8521 gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat acattttacc
     8581 ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg gaacagtcgg
     8641 tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc ttttaccagc
     8701 atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa acgcaccact
     8761 tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac tgccagcggt
     8821 tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct
     8881 tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca
     8941 aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg
     9001 aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata caggctaacc
     9061 gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc aaacaagata
     9121 gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc
     9181 aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat cagcataccg
     9241 ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact
     9301 aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg
     9361 ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc
     9421 ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat acccaccaga
     9481 gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc
     9541 gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta
     9601 agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg
     9661 acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc
     9721 catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa
     9781 tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt agctaacccc
     9841 ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt
     9901 ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca
     9961 tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc
    10021 attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt
    10081 actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat aggtccattt
    10141 ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt ttcactacgc
    10201 tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct ttcgttttca
    10261 gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc gaccataagc
    10321 gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata acgaggaaac
    10381 attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca aatattgtag
    10441 tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg cctgggcgct
    10501 ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta cctgctgtac
    10561 cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt ccctgatttc
    10621 cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag aagtctttga
    10681 agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta atccgccgct
    10741 gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga aactcaaacg
    10801 tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt
    10861 gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca tcaccgacaa
    10921 acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg ccatcattac
    10981 ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc cactgatgtt
    11041 ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg ccggagagtt
    11101 cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca aaatgggctg
    11161 caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg gcgtttcttt
    11221 cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg
    11281 ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca ccgccgatac
    11341 cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg cagttgccag
    11401 cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac atcagccagg
    11461 caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac catcaataat
    11521 gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat gcgctgtgaa
    11581 gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac catgtttcgc
    11641 gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt
    11701 atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat cgaagatgcc
    11761 atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct ggcaacgctc
    11821 tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg gatcgacgtg
    11881 ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag tctctcctct
    11941 tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa gaaattcgtt
    12001 gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca gtacaaactg
    12061 ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga tttccaacgt
    12121 gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc
    12181 ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc ggtcaatatg
    12241 gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat taacaacgtg
    12301 atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga aatcggcacg
    12361 ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc caacgacttt
    12421 ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt caacaatgtg
    12481 ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca cgcctggtat
    12541 cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga cccgtggtac
    12601 aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga acaggaacgc
    12661 tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg gccgctggca
    12721 cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg
    12781 atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg cattttgtgc
    12841 cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga caagctgatg
    12901 accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga acctgcaacc
    12961 tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc gttaggccac
    13021 tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc gaccacctgg
    13081 aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc gctgatgaac
    13141 accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca cagctttgat
    13201 ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct gatctccgtg
    13261 caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc
    13321 cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt atgcatggcg
    13381 gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag tggcgaggcg
    13441 acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg gatgattttt
    13501 accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta ctcccgcgag
    13561 ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc
    13621 tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc catcgcccag
    13681 gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg ttttgcgctg
    13741 tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt ggaatttttc
    13801 tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg gatgtggctg
    13861 attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat catgtccgac
    13921 gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg caaaataagt
    13981 aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc aacctgctgt
    14041 gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat taccactggc
    14101 ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg gggtatgtcg
    14161 aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa cctggaacgc
    14221 tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa atgagcctgc
    14281 atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat ctgccagcac
    14341 atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc agcctgagcg
    14401 aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag ttggctgcgt
    14461 ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac tgtctgtcga
    14521 tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag gagcagggat
    14581 gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc
    14641 ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt
    14701 aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga ttggcgaatt
    14761 actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga
    14821 agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt ttaccggcgg
    14881 aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg
    14941 cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg
    15001 gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt
    15061 caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag cccgcaggtg
    15121 attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc gttgggcgag
    15181 gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat caatacgctt
    15241 ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa aagtgaaata
    15301 caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg
    15361 gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat
    15421 ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg
    15481 ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat
    15541 ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct ctgtgggccg
    15601 ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta
    15661 cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact ggaaagttat
    15721 gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg
    15781 cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta aaaagacggt
    15841 aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt
    15901 caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc ctacaggagt
    15961 gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca
    16021 ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat
    16081 ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt
    16141 cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac
    16201 taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac tggcgatgga
    16261 agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct tcggctggca
    16321 acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct
    16381 ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt
    16441 tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct ttaacccggt
    16501 cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg ccatgtttat
    16561 tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg cattacgctc
    16621 gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc aactcggaga
    16681 cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga
    16741 gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca
    16801 agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg ccactcactc
    16861 attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca
    16921 acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc gtgagccgca
    16981 tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt tgctctcccg
    17041 ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc
    17101 gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct
    17161 gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca
    17221 tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc
    17281 cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac
    17341 atactccgcg cactccgctt taaccacagg acaactggct ttctcactga tcatgatcgt
    17401 agggctttac accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct
    17461 ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc
    17521 atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg
    17581 gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc gctggtggcg
    17641 cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg ggaaggcaat
    17701 caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag agtgtatgca
    17761 gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct gttcttccgt
    17821 ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa aatgtgggat
    17881 gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac
    17941 ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc
    18001 ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct tgggatggtg
    18061 attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca
    18121 cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcaggttac
    18181 tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc
    18241 catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa
    18301 tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct gacggtgatg
    18361 gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat gcaatttggc
    18421 gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag tgtgagtcca
    18481 atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag cattatgttg
    18541 gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa
    18601 atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt gtactaagga
    18661 gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt tcgctctcca
    18721 cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag gcctgatgcg
    18781 gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg aaacatatcg
    18841 atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc gcaatctgca
    18901 ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag tcgtcatggt
    18961 gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga cgcatggcca
    19021 acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat cgcctatctc
    19081 ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa gggctgcccg
    19141 cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa
    19201 gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca ggcagatacg
    19261 tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact ggataacgcg
    19321 aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt taccgggcat
    19381 cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc aaacttgtgc
    19441 cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc atcggaactc
    19501 aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg
    19561 gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg aaggatcacc
    19621 gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta tttactacaa
    19681 cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat catggcagcg
    19741 ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac ttcagtgctg
    19801 tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga gctcaactgg
    19861 acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt tgaacgctgg
    19921 cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca gactttacag
    19981 cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt gaaactgacc
    20041 ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa
    20101 atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa gagcattcag
    20161 ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa
    20221 aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt aactataacc
    20281 gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac cattgaggat
    20341 tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc tcacctcttt
    20401 ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc gtcccacgac
    20461 cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat cgtaatggtc
    20521 gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag acgggttagg
    20581 tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct ggatcacttt
    20641 gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac tcacggtaaa
    20701 caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt tttgatcgga
    20761 ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt ctggcgtcgc
    20821 accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca gactggttcg
    20881 tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca ggaaggggat
    20941 atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca gacgggtgcg
    21001 tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt gctcttccag
    21061 ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg cactcaccga
    21121 aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg gtttcactgg
    21181 ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac tggatttcga
    21241 aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc ccgcttctac
    21301 gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata cccgcttatt
    21361 caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac gataagttgg
    21421 gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct cattgagttg
    21481 attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa ggtcaaccga
    21541 atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct cgctgcgcac
    21601 ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg cctggcgagc
    21661 gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa tcaactgggg
    21721 atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt cgctaacggt
    21781 aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca ggacaaactg
    21841 tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt tttccagacg
    21901 ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt ggccgttttc
    21961 cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat gcatccagct
    22021 gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac ccacaatcgg
    22081 aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt taccgagaat
    22141 catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt tagggatcat
    22201 atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga ccagagtatc
    22261 tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc acgctgataa
    22321 taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc tcattttcct
    22381 gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat aaactcaaat
    22441 taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact gtagacatgt
    22501 tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc cagccattgc
    22561 ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg agcaatatgt
    22621 tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt gagcttacgt
    22681 ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc tgtcgcatct
    22741 gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc cggcagacgt
    22801 ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca aaccactagg
    22861 attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct gtcaggtcat
    22921 gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga gccagcggcg
    22981 cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg agtatcattg
    23041 ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc tgcttgtttt
    23101 gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc ataccctcag
    23161 cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc gtgctctgtt
    23221 tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata aataaaatat
    23281 cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc gcatagaggt
    23341 tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct gcatctgcgg
    23401 tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg gtaatcggct
    23461 gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg aacgccgcga
    23521 cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta atctcgttgc
    23581 gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg tagaaaatcg
    23641 caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc cctgcggggg
    23701 tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc ccgatacgat
    23761 attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata atcggtttgg
    23821 cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt ttgtccaggt
    23881 catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa ttctgaccag
    23941 gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa aacttaaaaa
    24001 tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt acgtcaggtt
    24061 attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat ttgcgtgaca
    24121 gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg ttgcggttag
    24181 gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta aagccgcctt
    24241 cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc gccacgccgc
    24301 tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga tattgcccaa
    24361 cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc gtcacgaaac
    24421 ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc tgcttcacgt
    24481 agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga agctcggaac
    24541 cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag aaaccattgc
    24601 ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg cgaacgtaat
    24661 cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt gaagacttaa
    24721 acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc aggtggtcgg
    24781 taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa tcttccgggc
    24841 cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct tcacgaatac
    24901 ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt tgcagttgct
    24961 ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg tcggtttcgg
    25021 tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca acgccttgcg
    25081 gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag tcacggtatt
    25141 tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct ggcgcgtcaa
    25201 agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac tgcaaacctt
    25261 cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca gggcgcagat
    25321 cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt tcataactga
    25381 ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa tggcttgcct
    25441 gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg agtttcagac
    25501 gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc gggccacgaa
    25561 agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga caatatttat
    25621 cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag ccccatgcca
    25681 tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat tcaccgtcaa
    25741 acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc cagtaacctt
    25801 cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga tcgccgctga
    25861 aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg gtgccttcca
    25921 gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta tcccggtagt
    25981 tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac aatcctgtgc
    26041 cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact tcagcatgac
    26101 aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc ttcatcagtc
    26161 aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat atttttcggg
    26221 cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt ccacatgtct
    26281 tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa tcaccataac
    26341 gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg taacgctacg
    26401 gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag taatagtcac
    26461 cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc tgttctcatc
    26521 cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat tgagattgag
    26581 taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt ctgcttccca
    26641 ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt tcgccgcaga
    26701 gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt gtgtcaccaa
    26761 ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag gcagataaat
    26821 agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc tggcaatgaa
    26881 atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac gggaataacc
    26941 cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc gacccagaat
    27001 gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca gacattacac
    27061 agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa agtagaactt
    27121 agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt ccagcggctg
    27181 ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt cacttccagc
    27241 agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac cagcatggcg
    27301 ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac cacaaagatt
    27361 cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg gatctgctga
    27421 gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc gaccatactt
    27481 tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat aagagggcgc
    27541 acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc ggtacgaata
    27601 tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt agcgcgtagt
    27661 ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg ctgcaactac
    27721 tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg tgccgacagc
    27781 gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag tggtcgttgt
    27841 ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg cagcaaacgc
    27901 agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt tcataacttt
    27961 tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct actttgagta
    28021 taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact gcgggtggga
    28081 aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat acgacttgtg
    28141 agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt acgaaagcta
    28201 ttaattattg ttggtaatat agttttaagt ggtactataa ctggctgtta aatggcattt
    28261 ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg gaaaagtaac
    28321 tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag tcagctttaa
    28381 acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc gcaggccatt
    28441 tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa
    28501 tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga atcctttgcc
    28561 gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac aaattcctta
    28621 ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga tatttaagac
    28681 cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa tagctgcgtc
    28741 ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat tatcaggcat
    28801 tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt aaagccctgt
    28861 ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAJVPT010000046        28557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVPT010000046 DAJVPT010000000
VERSION     DAJVPT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OQM47_001983"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OQM47_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1480149.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OQM47_001984"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OQM47_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1480150.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OQM47_001985"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OQM47_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1480151.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OQM47_001986"
     CDS             4843..5082
                     /locus_tag="OQM47_001986"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480152.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OQM47_001987"
     CDS             5092..5262
                     /locus_tag="OQM47_001987"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1480153.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OQM47_001988"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OQM47_001988"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1480154.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OQM47_001989"
     CDS             6724..6990
                     /locus_tag="OQM47_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCS1480155.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OQM47_001990"
     CDS             7084..7773
                     /locus_tag="OQM47_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1480156.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OQM47_001991"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OQM47_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1480157.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OQM47_001992"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OQM47_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1480158.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OQM47_001993"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OQM47_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1480159.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OQM47_001994"
     CDS             complement(10475..12193)
                     /locus_tag="OQM47_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1480160.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OQM47_001995"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OQM47_001995"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1480161.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OQM47_001996"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OQM47_001996"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1480162.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OQM47_001997"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OQM47_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1480163.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OQM47_001998"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OQM47_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1480164.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OQM47_001999"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OQM47_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OQM47_002000"
     CDS             18432..18746
                     /locus_tag="OQM47_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1480165.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OQM47_002001"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OQM47_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1480166.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OQM47_002002"
     CDS             20570..21862
                     /locus_tag="OQM47_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1480167.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OQM47_002003"
     CDS             complement(22016..23665)
                     /locus_tag="OQM47_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1480168.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OQM47_002004"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OQM47_002004"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1480169.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OQM47_002005"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OQM47_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1480170.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OQM47_002006"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OQM47_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1480171.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OQM47_002007"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OQM47_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         7165 a         7657 c         7234 g         6501 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataat
//
LOCUS       DAJVPT010000047        28394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVPT010000047 DAJVPT010000000
VERSION     DAJVPT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQM47_002008"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQM47_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1480172.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OQM47_002009"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OQM47_002009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1480173.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OQM47_002010"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OQM47_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1480174.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OQM47_002011"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OQM47_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1480175.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OQM47_002012"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OQM47_002012"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1480176.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OQM47_002013"
     CDS             6426..7055
                     /locus_tag="OQM47_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1480177.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQM47_002014"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQM47_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1480178.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OQM47_002015"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OQM47_002015"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1480179.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OQM47_002016"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OQM47_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1480180.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OQM47_002017"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OQM47_002017"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1480181.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OQM47_002018"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OQM47_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1480182.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OQM47_002019"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OQM47_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1480183.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OQM47_002020"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OQM47_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1480184.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OQM47_002021"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OQM47_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1480185.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OQM47_002022"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OQM47_002022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1480186.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQM47_002023"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQM47_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1480187.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQM47_002024"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQM47_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1480188.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OQM47_002025"
     CDS             complement(21746..22624)
                     /locus_tag="OQM47_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1480189.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQM47_002026"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQM47_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1480190.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OQM47_002027"
     CDS             25252..25587
                     /locus_tag="OQM47_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1480191.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OQM47_002028"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OQM47_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1480192.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OQM47_002029"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OQM47_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1480193.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OQM47_002030"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OQM47_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1480194.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28394
                     /locus_tag="OQM47_002031"
     CDS             28256..>28394
                     /locus_tag="OQM47_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCS1480195.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GN"
BASE COUNT         7294 a         7415 c         6933 g         6752 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aaca
//
LOCUS       DAJVPT010000048        27911 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVPT010000048 DAJVPT010000000
VERSION     DAJVPT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..460)
                     /gene="wcaB"
                     /locus_tag="OQM47_002032"
     CDS             complement(<1..460)
                     /gene="wcaB"
                     /locus_tag="OQM47_002032"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1480196.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVG"
     gene            complement(463..1302)
                     /gene="wcaA"
                     /locus_tag="OQM47_002033"
                     /pseudo
     CDS             complement(463..1302)
                     /gene="wcaA"
                     /locus_tag="OQM47_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(1395..3557)
                     /gene="wzc"
                     /locus_tag="OQM47_002034"
     CDS             complement(1395..3557)
                     /gene="wzc"
                     /locus_tag="OQM47_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1480197.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(3560..4003)
                     /gene="wzb"
                     /locus_tag="OQM47_002035"
     CDS             complement(3560..4003)
                     /gene="wzb"
                     /locus_tag="OQM47_002035"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1480198.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(4009..5148)
                     /gene="wza"
                     /locus_tag="OQM47_002036"
     CDS             complement(4009..5148)
                     /gene="wza"
                     /locus_tag="OQM47_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1480199.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            5457..5606
                     /locus_tag="OQM47_002037"
     CDS             5457..5606
                     /locus_tag="OQM47_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480200.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            5807..7390
                     /locus_tag="OQM47_002038"
     CDS             5807..7390
                     /locus_tag="OQM47_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1480201.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(7842..9695)
                     /gene="asmA"
                     /locus_tag="OQM47_002039"
     CDS             complement(7842..9695)
                     /gene="asmA"
                     /locus_tag="OQM47_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1480202.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(9717..10298)
                     /gene="dcd"
                     /locus_tag="OQM47_002040"
     CDS             complement(9717..10298)
                     /gene="dcd"
                     /locus_tag="OQM47_002040"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1480203.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(10390..11031)
                     /gene="udk"
                     /locus_tag="OQM47_002041"
     CDS             complement(10390..11031)
                     /gene="udk"
                     /locus_tag="OQM47_002041"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1480204.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            11349..11591
                     /locus_tag="OQM47_002042"
                     /pseudo
     CDS             11349..11591
                     /locus_tag="OQM47_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            11590..12531
                     /gene="mdtA"
                     /locus_tag="OQM47_002043"
                     /pseudo
     CDS             11590..12531
                     /gene="mdtA"
                     /locus_tag="OQM47_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            12531..15653
                     /gene="mdtB"
                     /locus_tag="OQM47_002044"
                     /pseudo
     CDS             12531..15653
                     /gene="mdtB"
                     /locus_tag="OQM47_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            15654..18719
                     /gene="mdtC"
                     /locus_tag="OQM47_002045"
     CDS             15654..18719
                     /gene="mdtC"
                     /locus_tag="OQM47_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1480205.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            18720..20135
                     /locus_tag="OQM47_002046"
     CDS             18720..20135
                     /locus_tag="OQM47_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480206.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            20132..21535
                     /gene="baeS"
                     /locus_tag="OQM47_002047"
     CDS             20132..21535
                     /gene="baeS"
                     /locus_tag="OQM47_002047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1480207.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            21532..22254
                     /gene="baeR"
                     /locus_tag="OQM47_002048"
     CDS             21532..22254
                     /gene="baeR"
                     /locus_tag="OQM47_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1480208.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            22445..22777
                     /locus_tag="OQM47_002049"
     CDS             22445..22777
                     /locus_tag="OQM47_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1480209.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            22924..24285
                     /gene="yegQ"
                     /locus_tag="OQM47_002050"
     CDS             22924..24285
                     /gene="yegQ"
                     /locus_tag="OQM47_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1480210.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24627..24944)
                     /locus_tag="OQM47_002051"
     CDS             complement(24627..24944)
                     /locus_tag="OQM47_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480211.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            25350..26249
                     /gene="yegS"
                     /locus_tag="OQM47_002052"
     CDS             25350..26249
                     /gene="yegS"
                     /locus_tag="OQM47_002052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1480212.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(26331..27104)
                     /locus_tag="OQM47_002053"
     CDS             complement(26331..27104)
                     /locus_tag="OQM47_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1480213.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(27210..>27911)
                     /locus_tag="OQM47_002054"
     CDS             complement(27210..>27911)
                     /locus_tag="OQM47_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1480214.1"
                     /translation="DGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQ
                     GCENKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEM
                     SAPQMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATF
                     GKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVR
                     DIARNAMQGKVLLIP"
BASE COUNT         6365 a         7413 c         7294 g         6839 t
ORIGIN      
        1 ctcccaccac cagcgcgttg tccgggacag agtcgagcac cacgctgccc gcacccacgg
       61 tgacgttgtt accaagcgtg atatcaccaa gaataatgac gttggcaccg agttcgacgc
      121 cgttgccaat gtgtggacat gccatgttat cggcaccccg attgccgata gtgacgccgt
      181 ggcgaatggt gaaatcatcc cccgccacca cgtttttatt gatcactacg gcgtaaccgt
      241 gatggatagt aaagcggcgg ccaatggtcg cggcggcctg gatttcataa ccgaaaaagc
      301 attcggtgat aatgcgatac agcaccagca gcggggccgc ccacagattg ttgaggacgt
      361 tctttttgcg ccacaccgaa caaaaatgag cgacgcgata ggcaagtacc atgcagcacg
      421 ggcgtaaact ccagctgttg gcgcgcagat cttccagcat ctttagcgcc cccgaatacc
      481 atcagccaga cgtttgccgt tacgcaccga cagcagcgtc aacaaggtgc gccaggtcat
      541 acgtttattg cggatctggt agagggtaaa cagctgatat tttttactgg cgcggtcgaa
      601 tttgtctttg tgcttgcggt aaaagtggaa gtatccggag aattttttcg gtgacgaggt
      661 gatctgcatt tcgccgtgat tgatatgcag gatctgcgtc gcctcttcca ctttccacgg
      721 ttcgccgtac tccaccacca tccgcaggaa tatgtcgtaa tcctgtgctg ctttcagttc
      781 ggtatcgaac aaacactctt tgaaacgcca tgcccaggta aagacctgat tgccaatgat
      841 attacgttta tagaacaggc ggcgtgagta cggcgatttg ggatacagtg gtaagctcgc
      901 cggttgggaa taaacttctc cctagcaaac gtagtcgtta gcgtacaaaa aggcgtgtgt
      961 gaccagttgc tgtttatggg cgaggaagac gctcaggcgg ttgggcgtcc attcatcatc
     1021 atcatcgatc ccggtgatgt attccccttg cgccagcata atcgcctggt tacgcaccgc
     1081 gcacgccccg ctgttaatgt cgttgtggat gtaagtgata cgcggatcgt tgagggcagt
     1141 gacgtactgt tgcagttgtt cccaggaggt ggagcaatca tccacgatga tcatctccca
     1201 gttgctgtag tcctggcgca ggaccgattt tatcgcccga atcgccagtt gttggcggtt
     1261 ccaggttggc atatagattg agatcagcgg attgtctttc ataggttgtt ctcccggctt
     1321 atgagctggt ttgcttgccg gatgcggcgt gagcgcctta tccggcctac ggggcggtgc
     1381 gaatgcaggc gtcgttattt cgcatccgac ttatattcgt attcgtaata gccataatcc
     1441 tgatacgcgc tggcgcggcg gaagatggag ttcagaatca cccctttcac cggaataccg
     1501 ttttgctcaa agcggctcag actggtttcc acttctttca atgtgttgac cgcataacgc
     1561 gccaccatta acgtggttcc gacatgacga ccaacaattg ccgcatcggt cactgccaga
     1621 atcggtggcg tatcaatcaa caccaggtca tagtttttac tcgcccagtt caccagttcg
     1681 gcaaagcgtt cgctcattaa cagttcagaa ggatttggcg gcacctgacc gcgcgggatc
     1741 aggtcaaatt tggcaataga ggtcggttta gcagcggtag taatatcgcc ctgaccaatc
     1801 agaatctccg acagcccatt gacattattg gtgcccaaca gctcgtgggt gtagcctttg
     1861 cgcatatcgc agtcgatcaa caacacgcgt ttattggtct ggctgatcac cgccgccagg
     1921 ttggcgcaga caaaggtttt accgattgac gggctaaccc cggtcatcat caacacattg
     1981 ttctgcgcct gcatcatcgc gaagtgcaga ctggtacgca gactacggat ggcttcaatc
     2041 gccagatcgg ttggattccc cactgccagt agctggctct gtttatagcg tttaacccct
     2101 ttgatggttt tgacgctatc gcgcgctttc tgccactccg acagcgggat gctggcatag
     2161 acgctgattc cgtgttcttc cagcacttgc gggctttcga tgccgcgatt aaacaacgag
     2221 cgcagcagca cacccacgat agagagcatc aggccaagga taatcgcgcc gaggataatc
     2281 agccctttct tcggttttag cacgccaggc tgggtgattg ccgggtcaat aatgcgcaca
     2341 tcgccgacgg tgctggcttc ggtgattttc agctcctgct ctttattcag cagttgcata
     2401 tagacctgct gaccagactc gacatcgcgg gtcagacgga caatttcctg ctggtttttc
     2461 ggcatcgccg ttacgcgacc gttaagtttg gctttctctt cttccagcgc ctgacgtttc
     2521 tccagcagcg tgcggtacgc cgggtgaact ttggtgtaca gcttggagat ttccgcctct
     2581 ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca tcgaatcgag caccgctttc
     2641 gcttcaagcg gcagatcaac agaatcttta tcctgacgga aggcattcag tttgttttcg
     2701 gcaacatcga ggcggctacg tacttccggt aactgttgcg cgaggaaagc gaggcttttc
     2761 gacgcttccg ccgatttgcg ctcaatattt tgttcctgat agttacgagc gatgctgtta
     2821 agaatgtcgc ggatctgttc gcgatcttca ccggtataag tcaggctcag tacgcctgcg
     2881 tctttgccgt tctccgttac cgtcaggctg ttttgcagtt ggttgatcat ccccaacgtg
     2941 gagtatttgg tgacggtaaa ctcactgccc gggcgggcgt gaatggcttc aaccatcagc
     3001 gtgacgcctt cttttttcag catctggccc gcttgcccac gggcgctaaa gccgccatca
     3061 ctgctcaggg tgtagttttt gtcatccagc acattaagcg taaacacctg atccgccatc
     3121 tctttcgggc ggttaaaggt agtcactttc accgtctcgt tctgacgccc catcaggcga
     3181 tcccagcccg ccccgaaaat cgggaacgtg tttttgctca ccgcaatatc gaggtcgaga
     3241 tcgtccaccg ttttaccaag caccagacgc gaacgaatca actggatctc ggcgtccgat
     3301 gcaggcggtt tgttggctaa tgccgagccg atatcctgca ccagcgaatt gccgctgttt
     3361 tgctcgattt gtaccagtgc gtcggcacta taaatcggcg tggcgaagaa agtgtaaacc
     3421 acggcacaga gggcaaatac gacggtgatg ccaatcaccc accagcgcgc ttcaatgacg
     3481 gtgccgacca ggcgaccaat atcgatttca tcactgcccg ttaccggagc ggcatgttgt
     3541 tttacttttt ctgtcattct tatacctgct ctgcgttcaa tgcctgcgcc cactggcggg
     3601 cagaccgttc aagtaatgtg tacaccgctg caaacgtttc ccggcttttg tgatacggat
     3661 cggggatttc acattcgtta tcccagtgac caaacagcat cactttgccg cgcatctccg
     3721 gtgccatctc gcataagcgt tcgatatggc gcttatccat ggtcaaaatc aggtcatagt
     3781 tgcgacacag gcggcggctg atttgtcgtg cacagtggcc ttccagagac agttgatgtt
     3841 ctgcggcgac gctgatagcg gtaggatcag cgcccttacc gaccagcgcg ccgagtccag
     3901 cggactccac tttcagttcc gggtgataac gttgcagtaa gcgttccgcc atcggggaac
     3961 ggcaaatatt gccgacacag acaactaaga tgttgttaaa catgacgatt accagttatg
     4021 aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta atagttggca gcaactgatt
     4081 gatcagacgg ttccagcggg aaaccggggc ggtggtgaca tacaccacgt cataaggttg
     4141 caggcggaat tctgtcgcca tcaccagcga cgtcgcatcg gacatatcca gctggtagat
     4201 attggcaatc ttgccgttac gcccgccctc gcctttcagc ggacgaatga caaagatgcc
     4261 gctggcgttg gaggtggtca tgtcgatacc ttcggcattg gccagagctt cagtcagggt
     4321 catgccgcta aagtccattt tcagggtgct ctgtttcttc acttcaccca tcacaaatac
     4381 tttcagatca tcattacgcg gcacgtagag aatatcgccg ggataaagca gtcggttctg
     4441 attgaggtcg ccgttttgca tcagcgcctg taaagaaatg cgttcttcac gaccattgtg
     4501 tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg cgttgatggc
     4561 gtcgagaatg gtcagtggta cgttggtgat cgcctgttga ccggatttat tcacctgacc
     4621 tgagatatag gccttttgtg agcggaaggc ggcgatatta acgtccacct gcgggtcagc
     4681 gatgtacgtc gctaagcgcc cggtaatatc actgcggatt tcagcgagcg ttttcccgac
     4741 tacgtgaacc ttgccgatat acgggtaaaa catagtgccg tcaggctgta cccagttgcc
     4801 ggtgtcgctg gagctgcggt actgaccagc tggcgtggtg agttccgggt gatcccagac
     4861 ggtgacatta agaacgtccc ccggcccgac gcgatactgg taattcgcga tctcgctttc
     4921 cagcgtcata ttggggcgcg ctacattcgg gcgtgggcgt aattggtcaa tcaggcgcgg
     4981 ggtcagcgga taaacattca ccattttgtc gagatcgaaa tcagcgtcct gctgtttgat
     5041 gacgtctttg cccatcgtcg acatattgct gcccggaagt actgtgcaac cgcttatcaa
     5101 ggttactgac accaataatg gcatcaattt cattttggat ttcatcattg tttatttatc
     5161 actttggcag agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc
     5221 ttgcagttaa ttgaataaaa atttaactgg catcagtcct aaaaaaattg atttcatccg
     5281 caggctattg acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc
     5341 tggcttttta gctaccaata cactgattga gcttaatttt tcaaaccctc tctgcataca
     5401 atcgttgatg agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg
     5461 taattgaata ttggctcgcc aataatgcag gaggaagtgt tacagctaac ggaatagcag
     5521 gcaaggtaac aattcggcaa ttggctattt ttaagaatta tattaagtat cattcaatat
     5581 ggtttttagg agtttcttta ggttgacaat atttaatatt gcgtctccac atgcgatatt
     5641 tcttaaataa tgttttatta ttaccgcttt taattcaggg ataatgtgag gttattaccc
     5701 tcaaataata tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga
     5761 tcgaattgtg acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc
     5821 cgatccgtct atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat
     5881 tgataacctg gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg
     5941 cgcacgggtt accgggctgc tgctggcgat gttaatgcgc ctgttactgc tagcgtcaat
     6001 ctcctggctg gtcaccctga ctcaaccgct gttcagcttc cgctcgttta cctttagcgc
     6061 ccgcgactta atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct
     6121 gaacgaacgg ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt
     6181 ctggggcgtg gtgacgcaaa ttgtggtact ggacgccatt ttctcactcg actcagtgat
     6241 taccgccgtc gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat
     6301 cagcctgatg ttgatggcca gcaagccgtt aacgcaattt gttaacagtc acccgaccat
     6361 cgttattctc tgcttaagct tcctgttgat gattggcttt agcctggtgg cagaaggttt
     6421 cggcttcgtc attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga
     6481 ggcactcaat cagttggcta tctttaaccg gcgacgtttt ctttccgcta atcagacgct
     6541 acgccagcgg actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga
     6601 actggacgcc aaaaccgcgt ccatgctggt ggatcatgga aaccagcaga tctttaatcc
     6661 gcaggaacgg cggatgattg agcgggtact taatcttaac cagcgtaccg taagcagcat
     6721 tatgacgtcg cgccacgata ttgagcatat cgatctcaac gcgccggaag aagaaatccg
     6781 ccagttgctg gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga
     6841 agatttgctc ggtgttgttc acgttatcga cctgctacaa cagtcactgc gcggcgaacc
     6901 gctcaacctg cgggtgttga ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt
     6961 accagccctg gagcagttcc gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt
     7021 tggctcggtg gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt
     7081 accgaacgaa gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc
     7141 ctggacggcg aatggtcata tgccgctgga ggatctggtg caatatgtgc cgctgccgct
     7201 ggatgaaaaa cgtgaatatc acaccattgc cgggctgttg atggagtatt tgcagcgtat
     7261 tccaaaacct ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga
     7321 aagccatcgc gtgcagaagg tgcagattat accgctgcgt aaggatggcg agatggagta
     7381 cgaggtgtga tggtatcggc cattttaccc aatgctgcgt aacgccttat aacttcagca
     7441 atgaagcgtt gtctgtggta aacggacatc acagcggggg tgtgggtccg gctgtaaatc
     7501 gccgaatcgt ttcctgtagg ctggggcagc ccacatggat gtgggctgag ggcgcgtttt
     7561 acagggatgt tacctcgcgc ccgtacccga tagccgaaag gaataagatg agggaaccgc
     7621 gccagcggcg atttaccgcc gggagcccgg gttgccaggg tggtggcgat tgagccaccc
     7681 tggcacgttc accggcttga gcgttccata gtagcgagga acatcaagtg aacggaacag
     7741 ccttcgatct tacatattca gaatataata gtgccgccgc gaacgcctga tgcgacgctt
     7801 tacgcgtctt atcaggccta caggacaggt cacctggtaa cctacatctt ctccagcaac
     7861 ttcttcacat ctttgccatt gcgggaatct ttattccgct cggcccagtc attcaggcga
     7921 cgtttcgctt cgtcctgtag atgtttgcgc agtaactgat ccacttgcag gctgtaattg
     7981 agttgctgcc agttgccata aacccgcagc ggtactggcg tttctttgag gaaatcaatc
     8041 agtttgcttt ccccgttcca gccacccacg acccgaatat caaactgggt gtcgcaggtt
     8101 tgatctgcca gattcaacat gccttcccct gtcagcgcca gcacaggcga ttgaccttgc
     8161 atgtcgttta acgtcacgac gccatccttc aacgtcaaat cggtggtaaa gcggtcaagc
     8221 cgcgtcacgt tatcgaagtt ttcagcggcc ttcacatcac cgccattacg ctctaccgct
     8281 tgctgaatca tctgctggaa gttcatccct tccatacgtg tgtcggtcat ctcaacatgc
     8341 gcctgtcctt gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca
     8401 ccagccagtg acatttttcc ggtcaacgaa atcggatagt taaacgcctt cagaatggta
     8461 ccaatctcaa cgttctccag ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg
     8521 tccagcgtgc ccggcagtga aacctttcca ccgttaagct tgccctgcag ttgagtaatt
     8581 tccagcaaac ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc
     8641 cagcgcacgt tactggcctg caacaaaata tcagctgtaa agccttgcag tccctgatag
     8701 gccggttcat caatacgcga agaaatgacc gggcgcggca acgttgattg gctctgcccc
     8761 tgctgcgcgg caccgttttc accattcgcg gtttcattaa gcgggatgag gttgtcaaga
     8821 ttcagttgcg ggaattgcag tctcagctgc cattccggtt tctctgtcag cgtgacctgt
     8881 gcttgtccgc tcagcgtact gtcattggcg gtcaaactaa tttggttaaa tgaaaggcgt
     8941 ttatgtgact cctgccactg ggactggaaa ctcccctgcc cctgaatacc ttgttttggt
     9001 aaatcggcac cctgcaactg ccagttaatt tgttcaatag ccgctgttaa atcatgcgga
     9061 taatcagaag catctaccgt accgttaagg gatatcgtga gatcgcgctg atcgcgatta
     9121 acccgcccgg agaactcaaa tgagccacga tgttggggat cttgttccat ttgcaggcgg
     9181 atattgcgga ttgtcacctg ctcgtcatct tcatgctgga acaccagaac gctgtccgcc
     9241 accttaagac tggatatatc aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta
     9301 ttgtcgcgcg gtgcaaccgg agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt
     9361 tggatcactg ccccttttag catcacctgc ttaacgctca gttgatgact cagtagtggt
     9421 aaaagcgcca cgtccagacg catgttgtcg gcgcgaacca gtggctggct tgcgccctgg
     9481 gcggtgagag acattcgccc ggagaggata ctaagctgcg gccagacgtg ccaacgcagt
     9541 ggcccgtcga gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac catatagtcg
     9601 cggaaatcat tcggattcac cagcaacact aacgcagata acccggccac cagcacgacc
     9661 aagagtatca tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg cctgaattag
     9721 tctttatcga ttcggctggc taccgcgccc tgctggttgc gatatttcgc atcttcacgg
     9781 cggttgtaag gtcgcgccgc cgggccggaa agcggctcaa agctcagcgc accaattaac
     9841 atgcccggac gcagcgccag cggcagctta ccggagttgt agaactccag cacaatgcaa
     9901 ccagaccagc ccggatcgat acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc
     9961 aaagaggaac gcccgtccag ccagcccacc agatcggctg gcagcgtcac cgactccagt
    10021 gtcaccgcca gcgccagctc tcctgggtga agatagaacg cctcgccctc gtcgagaacg
    10081 atctcatcgc tcatcacgcg gtcaagcgcg gcgctcactt catctttggg accgctcaga
    10141 tcgataaacg ctgccgtgtg accacggaag gtacgaaatt tattgcccag gcgtacatcc
    10201 accgtcgcgc cgttaatacg ctccactggc ggacgtgggt tgatcgacaa acggccttca
    10261 tcaagccagg cttcaatatc tcggtcacac agacgcatgg cattttctcc tttcgcacat
    10321 cactccctta acgccaatta cgtcaagggc ataccaactt atcactgaac ggtacacaat
    10381 ttatcaagct tattcaaaga actgacttat tttcgctttc aatatatcga tcgcgatgcg
    10441 gtttttcccg ccgcgcggca cgataatgtc cgcatattgt ttagaaggct caatgaattg
    10501 caggaacatc gggcgcacgg ttttttgata ttgcgccatc acggaatcca ttgaacgccc
    10561 acgctcgtta acgtcacgct tgatgcggcg catcaggcag atatccagcg gggtatcaac
    10621 gaaaatggag aagttaagtt cgtcacgcaa acgcgcatcc gtcagcaaca aaatgccttc
    10681 gagaatgatg accttcttcg gctcaaccgt caccgtttct ttcatacgcg tatgttcaac
    10741 atagctgtaa accggcaggt caattgccga gccgcgtttc aacgcttgta aatgctcaag
    10801 caacaggctg tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc gttcttccat
    10861 cgacagatgg ctttgatctt tgtaatagca gtcttcggga attacgccga tgtgttcatc
    10921 accgacttgc tcacgtaatt cacgataaag ggtactggca ataagactct tgccggaagc
    10981 cgatgcgcca gcgataccga taatgacgca ctgatgagac tgatcagtca tatatttagc
    11041 gacctgatta acctggatgt taggaagggg gcgacgaagc gccaaacgcg gcaattatag
    11101 ggatttcatc cgtctgatac cagtcgaata gcgttgccgc gcgctcagag ttaattgttg
    11161 acaaagaatt cccggtggca aattacgttg atcagtttta tgtaaggtaa aaaatgttat
    11221 acgcagttgc gcaaattatc cgcctttacg tcactttatg agcaattcgc atataaaatg
    11281 taaaactttt gtactagcat aaacacagaa acgaatactg gcgacctggc cttgcggata
    11341 aagcggtaat aagcaaacaa tcacagcatg tattaattgc cctgccccac ccgctgcttc
    11401 acctggtcag tttaggttta gtctcgttta tctttaccct tgtctcgctt gagctttcgc
    11461 agtttggcac ccaactcgcc ccactgtggt tcccgacgtc catcatgatg gtggcgtttt
    11521 atcgccatgc cgggcgcatg tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg
    11581 ccgcatccat tccaggaagg ccagcaggtc aaagcaggcg atttactggc agaaattgac
    11641 cccagccagt tcaaagttgc attagcacaa acccagggcc aactggcaaa agataaagcc
    11701 acgcttgcta acgcccgccg tgatctggcg cgttatcagc aactggcaaa aaccaatctc
    11761 gtctcccgtc aggagctgga tgcccaacag gcgctggtca gtgaaaccga aggcaccatt
    11821 aaggctgatg aagcaagcgt cgccagcgcg cagttgcaac tcgactggag ccgcatcacc
    11881 gcaccagtcg atggtcgcgt tggtctcaag caagttgatg ttggtaacca aatctccagt
    11941 ggtgatacca ccggaattgt ggtgatcacc cagacgcatc ctatcgattt agtctttacc
    12001 ctgccggaaa gcgatatcgc tactgtagtg caggcgcaaa aagccggaaa accgctgatg
    12061 gtagaagcct gggatcgcac caattcgaag aaattaagtg aaggcacgct gttaagcctc
    12121 gataaccaaa tcgatgccac aaccggtacg attaaagtga aagcacgctt taataatcag
    12181 gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca tgttagtcga caccgaacaa
    12241 aacgccgtag tgatcccaac agccgccctg caaatgggca atgaaggcca ttttgtctgg
    12301 gtgctgaata gcgaaaacaa ggtcagcaaa catctggtga cgccgggcat tcaggacagt
    12361 cagaaagtgg tgatccgtgc aggtatttct gcgggcgatc gcgtggtgac agacggcatt
    12421 gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag cccagagcgc caccactccg
    12481 gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag cacgctcctg atgcaggtgt
    12541 tacccccgag cagcacaggc ggcccgtcgc gcctgtttat tatgcgtcct gtcgccacca
    12601 cgctgctgat ggtggcgatc ttactcgccg ggattatcgg ttatcgcgcc ctgcccgttt
    12661 cggcgctgcc ggaagtggac tatccgacca ttcaggtggt cacgctctac ccaggtgcca
    12721 gcccggatgt catgacctct gccgttaccg cgccgctcga acgccagttc gggcagatgt
    12781 ctggcctgaa acagatgtcg tcgcaaagtt ccggcggtgc gtcagttatc actttgcagt
    12841 tccagctaac attaccgctc aatgtcgccg agcaggaagt gcaggccgcg attaacgctg
    12901 cgaccaactt gttgccgagc gatctgccta acccgccggt ttacagcaaa gtgaacccgg
    12961 cagatccgcc gatcatgacg ctcgccgtca cctcaaccgc catgccgatg acgcaagtgg
    13021 aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca gatttccggc gtcggcctgg
    13081 tgacgctttc cggcggtcag cgtccggctg ttcgcgtcaa acttaacgct caggcgattg
    13141 ccgccctcgg cctgaccagc gaaaccgtgc gcaccgccat taccggcgct aacgttaact
    13201 cggcaaaagg tagcctcgac ggcccttccc gtgcggtcac gctttccgcg aacgaccaga
    13261 tgcaatccgc cgaagagtat cgccagctaa tcatcgccta ccagaacggc gcgccaattc
    13321 gtctgggcga tgtcgcaact gtagagcaag gtgcagaaaa cagctggctc ggcgcgtggg
    13381 cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca gcccggtgct aacattatct
    13441 ccaccgccga cagcattcgg cagatgctgc cacagctcac tgagagtctg ccgaaatcgg
    13501 tgaaggtgac agtgctttcc gatcgcacca ccaatatccg cgcatccgtc gatgatactc
    13561 agtttgaatt gatgatggct atcgcgctgg tagtcatgat tatctacctg tttttgcgca
    13621 atattccggc gaccatcatt cccggtgttg ctgtaccgct gtcgttaatc ggcactttcg
    13681 cggttatggt gtttctcgat ttttcaatca ataacctgac actgatggct ttaactatcg
    13741 ccaccggatt cgtggtcgat gacgccatcg tggtgatcga aaacatttcc cgctatatcg
    13801 aaaaaggcga aaaaccgttg gcggcggcgc tcaagggcgc aggtgaaatc ggctttacca
    13861 ttatctcgct gaccttctca ctgattgcgg tgttgatccc actgctgttt atgggcgata
    13921 tcgtcgggcg actgttccgc gaatttgcta ttaccctggc ggtagcgatt ttgatctcag
    13981 cggtggtgtc gctgaccctg acaccgatga tgtgcgcgcg gatgctcagc caggagtcgt
    14041 tgcgtaaaca gaacagcttc tcccgtgcct cggaaaaaat gttcgacagg ataatcgccg
    14101 cctatggtcg tggactggcg aaagtgctga atcatccgtg gctgacctta agcgtggcac
    14161 tcagcacgct gctgcttagc gtgctgctgt gggtgttcat tccgaaaggt ttcttcccgg
    14221 tacaggacaa cggcattatt cagggcactt tgcaggcacc gcaatccagc tcctttgcca
    14281 atatggccca gcgacaacgc caggtcgcgg acgtgatttt gcaggatccg gcagtgcaaa
    14341 gcctgacctc atttgttggc gttgatggca ctaacccgtc gctgaatagt gcacgtttac
    14401 aaatcaacct caaaccgttg gatgaacgtg atgatcgggt gcaaaaagtc atcgcccgtc
    14461 tgcaaacggc ggtagataaa gtgccgggcg tcgatctctt cctgcaacca acgcaggacc
    14521 tgaccatcga tactcaggtc agccgcaccc agtaccagtt taccttgcag gccacgtcac
    14581 tggatgcgct cagtacctgg gtgccacagt tgatggaaaa actccagcaa ctgccacagc
    14641 tttctgatgt ctccagcgac tggcaggaca aagggctggt ggcgtatgtc aatgttgatc
    14701 gcgacagcgc cagccgtctg gggatcagca tggcggacgt cgataacgcc ctgtacaacg
    14761 cgtttggtca gcgactgatt tccactattt atactcaggc taaccagtat cgcgtggtgc
    14821 tggagcacaa taccgaaaat accccaggcc tcgcggcgct ggataccatt cgcctgacca
    14881 gcagcgacgg tggcgtggtg ccgttaagct caattgccaa aattgagcag cgttttgcgc
    14941 cgctttccat caaccatctg gatcagttcc cggtaacaac catctccttt aacgtgccgg
    15001 ataactattc gctgggcgat gcggtgcagg cgattatgga caccgaaaag acgctgaatc
    15061 tgccggtgga tatcaccacg caattctagg gcagcaccct cgccttccag tcggcgctgg
    15121 gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat gtatatcgtg ctcggcattc
    15181 tgtacgagag ctttattcac ccgatcacca ttctctcgac gctacccacc gcaggggtcg
    15241 gcgcgctgct ggcattaatg attgccggta gcgaactgga tgtgattgcg attatcggca
    15301 ttattttgct gatcggtatt gtgaagaaga acgccatcat gatgatcgac ttcgcgctgg
    15361 ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat ctaccaggct tgtctgttgc
    15421 gttttcgtcc gatcctgatg accaccctgg cggctctgct tggcgcgctg ccgctgatgt
    15481 tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg tatcggcatg gtcggcggtc
    15541 tgattgtcag ccaggtgctg acgctgttca ccacgccggt gatttatctg ctgttcgacc
    15601 gcctggcatt gtggaccaaa agtcgctttg ctcgccatga agaggaggcg taagtgaagt
    15661 tttttgccct attcatttac cgcccggtgg cgacgatttt actgtcggtc gccattaccc
    15721 tgtgcggcat actgggtttc cgtatgctgc cggtcgcccc gctgccgcag gtcgattttc
    15781 cggtgattat ggtcagcgcc tcgctgcccg gtgcgtcgcc ggagacaatg gcgtcttccg
    15841 ttgccacgcc gctggagcgc tcacttgggc gcattgccgg ggttagtgaa atgacctcca
    15901 gcagttcgct cggcagcacg cgcattattt tgcagtttga ttttgaccgg gatatcaacg
    15961 gcgcagcgcg tgatgtgcag gcggcgatca acgctgcaca aagtttgctg cccagcggga
    16021 tgcccagccg cccgacctat cgcaaagcga acccgtcgga tgcgccaatt atgatcctca
    16081 cgctgacatc cgatacttat tcgcagggtg aactgtacga tttcgcctcg acgcagctgg
    16141 ctccgacgat ttcgcaaatc gacggtgtcg gtgatgtcga tgtcggtggc agctcgctgc
    16201 ccgccgtgcg cgtcgggctg accccgcagg cgctgtttaa tcagggcgtg tcgctggacg
    16261 acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc gcagggggcg ctggaagatg
    16321 gcactcaccg ctggcagatc cagaccaacg atgaactgaa aaccgccgct gaatatcagc
    16381 cgctgattat tcactacaac aacggcggcg cggttcgtct gggcgatgtg gcgacggtga
    16441 ctgactcagt gcaggatgtg cgcaacgccg ggatgaccaa cgccaaaccg gctattttac
    16501 tgatgatccg caaactgccg gaagccaata ttatccagac ggtagacagc atccgggcaa
    16561 aattaccaga gttgcaggag accattccgg cggcgattga tctgcaaatt gcccaggatc
    16621 gctcccccac cattcgcgcc tcgctggaag aagtcgagca aacgctgatt atctcggtgg
    16681 cgctggtgat tctggtggtc tttttattcc tgcgctcggg tcgcgccact attattcccg
    16741 ccgttgcggt gccggtttcg ctgattggta cgtttgcggc gatgtacctg tgcggattca
    16801 gtctcaataa tctttcgctg atggcgctca ccattgctac tggtttcgtg gtggatgacg
    16861 ccattgtggt gctggaaaac attgcccgtc atctggaagc ggggatgaaa ccactacagg
    16921 ccgcgctgca aggtactcgc gaagtcggct ttacggtgct gtcgatgagt ctgtcactgg
    16981 tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc gggccgactg ttacgcgagt
    17041 ttgccgtgac gctttctgtc gccattggta tttcgttgct ggtttccctg acgctaacgc
    17101 caatgatgtg cggctggatg ctaaaagcca gcaagccgcg tgagcaaaag cgactgcgtg
    17161 gttttggccg catgttggta gccctgcaac aaggctacgg caagtcgctg aaatgggtgc
    17221 tcaatcatac ccgtctggtg ggcgtggtgc tgcttggcac cattgcgctg aatatctcga
    17281 tcccgaaaac cttcttcccg gagcaggaca ccggcgtact gatgggtggg atacaggcgg
    17341 atcagagtat ttcgtttcag gcgatgcgcg gcaaattgca ggatttcatg aaaattatcc
    17401 gtgacgatcc ggcagtggat aatgtcaccg gctttaccgg cggttcacga gttaacagtg
    17461 ggatgatgtt tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta
    17521 tcgaccgtct gcgcgtgaag ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg
    17581 tacaagatat tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat
    17641 ccgacgacct ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt
    17701 tgccggaact ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg
    17761 tttacgaccg cgacaccatg gcacggctgg gaatcgacgt acaggccgcc aacagtctgt
    17821 taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata
    17881 aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt
    17941 tcgttatcaa taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg
    18001 cgaatgcccc actatcggtg aatcatcagg gattatcggc ggcctcgacc atctcgttca
    18061 acctgccgac cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc
    18121 agcttggtgt gccttcgacg gtgcgcggca gttttgccgg cacggcgcag gttttccagg
    18181 agacgatgaa ctcgcaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc
    18241 tgggtatcct ttacgagagt tacgtacatc cgctgacgat tctctccacc ctgccctcgg
    18301 cgggcgttgg agcgctgttg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc
    18361 tgatagggat catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt
    18421 ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct
    18481 gtctgctgcg ttttcgcccg attatgatga ccaccctggc ggcgctgttt ggcgcgctgc
    18541 cgctggtatt gtcgggcggt gacggctcgg agctgcggca acccctggag atcaccattg
    18601 tcggcggtct ggtgatgagc cagctcctga cgctgtatac cacgccggtg gtgtatctct
    18661 ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa
    18721 tgacagatct tcccgacagc acccgctggc aattatggat tgtggctttc ggcttcttta
    18781 tgcagtcgct ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg
    18841 gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga
    18901 tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg
    18961 ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac
    19021 tgttgctggc acgcgcgtta cagggcgttg gcggtgcgat gatggtgccg gtcggcaggt
    19081 tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt
    19141 tacccggtca ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtagaatacg
    19201 cgtcgtggca ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca
    19261 cattgatgtt aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt
    19321 tattgctggc ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag
    19381 gtttatcgcc gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt
    19441 atctgctgca cgccagaaat aacaaccgcg ccctgttcag tctgaaactg ttccgcactc
    19501 gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc
    19561 cctttatgac accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac
    19621 tgatgatgat cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gtacaggtgg
    19681 tgaatcgctt tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca
    19741 ccctgttgtt tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt
    19801 ttttacaagg gatggtcaac tcgacccgtt tctcctccat gaacaccctg acgctgaaag
    19861 atctcccgga caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt
    19921 cgatgagtat cggcgtcacc atcgccgggc tgttgctggg gctttttggt tcgcagcata
    19981 tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt
    20041 ttatcatcgc ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg
    20101 tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg
    20161 caaactgttt ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc
    20221 ggtgcgtatc agttttgagc gtggctttat tgattacatc aaacacggca atgaacagcg
    20281 attacagctg ttaagtgatg cgcttggcga gcagtatgcg cagcatggta actggcgctt
    20341 cctgcgcaac aacgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc
    20401 ggaagataaa cccggcccgg gtatgccgcc gcacggctgg cgtacccagt tctgggtggt
    20461 cgatcaaaac aacaaagtac tggttggtcc gcgagcgccg attccacctg acggtacacg
    20521 gcgacccatt ctggtcaacg gtgcggaagt gggcgcggtg attgcctccc ctgttgagcg
    20581 gctgacccgc aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat
    20641 cgtcgccctg gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct
    20701 ggcaccggta aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac
    20761 ccgcgtaacg cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct
    20821 cgccagcacg ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca
    20881 cgaactgcgt acgccattag cggtgctgcg cggtgaactg gaagccattc aggatggcgt
    20941 gcgcaaattc acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa
    21001 actggttgac gatcttcatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa
    21061 agcaccggta gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt
    21121 cgccagccgt ggcctgaaac tgcaattttc cctgccagac agtatcaccg tatttggcga
    21181 tcgcgaccgt ttaatgcagt tattcaataa cttactggaa aacagcctgc gctacactga
    21241 cagcggcggc agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt
    21301 tgccgacagt gcgccaggtg tcagtgacga tcagctacaa aaattgtttg aacgttttta
    21361 tcgcaccgaa ggttcccgca accgcgccag cggcggttcc gggctggggc tggcgatttg
    21421 cctgaacatt gttgaagccc ataatggccg cattattgcc gcccattcgc cttttggcgg
    21481 ggtaagcatt acagtagagt taccgctgga acgagattta cagagagaag tatgaccgag
    21541 ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg
    21601 cagttgctca ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc
    21661 gatcaggtac tggcgtatgt gcgccagaca ccaccggatc tgatcctgtt ggatctgatg
    21721 ctccctggca ccgatggcct gacattatgc cgggaaattc gtcgtttttc cgatgttccg
    21781 atcgtgatgg tgacggcaaa aatcgaagag atcgaccgcc tgctggggct ggagattggc
    21841 gccgatgact acatctgcaa gccgtacagc ccacgggaag tggtagcgcg tgtcaaaacc
    21901 attttgcgcc gttgtaaacc gcaacgcgaa cttcagcaac aggatgccga aagcccgttg
    21961 attatcgacg aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct
    22021 gcggaatttc gtctgcttaa aacgctctct cacgaaccag gaaaagtgtt ctcccgcgag
    22081 caattgctca atcatcttta tgacgactac cgcgtagtaa ccgaccgcac cattgacagc
    22141 cacattaaaa atctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc
    22201 gccgtttacg gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag
    22261 cgacattact ttgttagccg gagatggcgt tcagacccga tccggcaaca ttattccacg
    22321 catggtcagc aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac
    22381 tttaatcagt aaacagcacc tttagatgct gttttcgata agcaatttca atcaaggagt
    22441 cattatggct ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct
    22501 aaaagcgggc aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc
    22561 ggaaaagggc atcgcgtcgg tgcgtagcaa cagcccgcaa gaagagcgct atgagaaaaa
    22621 aacggcaagc aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg
    22681 ctccagccag atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc
    22741 caatggcaca agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt
    22801 tggcgtccgg cgctttacca gatcccccct acagcgctac aatgcccgcc cttaaagtgg
    22861 gggcctcccc taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga
    22921 attatgttta aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct
    22981 ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc gttactccct acgtgtgcgc
    23041 aacaacgaat tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg
    23101 aaaaagtttt atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc
    23161 cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg
    23221 ctgattatgc tggtgcgtga gcacttccct gaaatgccga tccacctctc ggtgcaggct
    23281 aacgccgtga actgggcgac ggtgaaattc tggcagcaaa tgggactgac ccgcgtgatc
    23341 ctctctcgcg agctgtcgct ggaagagatt gaagagatcc ataatcaggt gccggatatg
    23401 gagatcgaga tcttcgttca cggcgcactg tgcatggcct actccggtcg ctgcctgctc
    23461 tctggctata tcaacaagcg cgacccgaac cagggcacct gcaccaacgc ctgccgctgg
    23521 gagtacaacg tccaggaagg gaaagaagat gacgttggca acatcgtaca caagtacgag
    23581 ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg gcgcgccaac cgacaaagtg
    23641 tttatgatcg aagaagccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac
    23701 ggcacttaca tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc
    23761 aaaatgggcg tgcattcgct gaaaatcgaa ggtcgtacca aatctttcta ctattgcgca
    23821 cgcaccgcac aggtttaccg caaagctatc gatgacgccg ctgcgggaaa accgttcgat
    23881 accagcctgc tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg
    23941 cgtcgtcata ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc
    24001 cagcagtttg ttggtgagtt taccggtgag cgcaaggggg acctcgcagc ggtagcggtg
    24061 aaaaataaat tctccgttgg cgacagtctt gagctgatga cgccgcaagg caacattaac
    24121 tttacccttg agcacatgga aaacgccaaa ggcgaagcta tgccggtcgc accaggcgat
    24181 ggttatactg tgtggctccc ggtcccgcag gatcttgagc taaattacgc gctgctaatg
    24241 cgtaatttct ccggggaaac cacgcgtaac ccccacggta agtgattaat ttcgattatt
    24301 tttcccggat ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc
    24361 atccgtttcg ctgaaaaaca taatccataa aatgctagct gtaccaggaa ccacctcctt
    24421 agcctgtgta atctccctta cacgggctta ttttttgtac ataagctgta aagaaggatt
    24481 gatccctgat aaccgtcttc cattgaccac atcgatagaa tcccccttca tagcacaatg
    24541 cctttcacgt aacggtatcg tgctcgccca ggttccggct acgcacagcc agaacgcgca
    24601 tgtttgacgc ttacaaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg
    24661 ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta
    24721 acacagaaat gggctgcatc attttttact atattttctg caccagatat tctgcccctg
    24781 gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt
    24841 ttatatgcaa gaccaaattt accgatactt gtttcatcat tatgcacagc acaaccagat
    24901 ataagaaaaa tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt
    24961 aaataccaac gacactctct agtgtttaaa tataataatg gcatgattat tataattgaa
    25021 taggattata ataaatgttc tgtacaacat ttcctacata agtaggaatt acggacattg
    25081 aggcccttca gggtaactcc atgggggctt taatatatta tattgaagat gccactgttt
    25141 agttgaatat taggtatatg ctcttttttg aaatttatcg gtggcagccg ttagtattcg
    25201 ctgtccccat tgcaagctcc tggtggtaac cactgaatcc tccatacttg aactgacttt
    25261 ttatcctccg actttcatcc tgttctgact ccaccttttg ttttctgctc tacactatct
    25321 acagaccaat cataaaggca catacgatca tggcagaatt tcccgccagc ttactgattc
    25381 ttaatggtaa aagtactgac aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg
    25441 aaggaatgac gatccatgtg cgggtcacct gggagaaagg tgatgccgca cgatttgtag
    25501 aggaggcccg gaagttgggc gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta
    25561 atgaagtttc tacggcgttg attcagtgtg agggggatga catacccgcg ctgggaattt
    25621 tgccattagg aaccgccaat gattttgcca ccagtgtagg gattcctgag gcactggata
    25681 aggcgctgaa actggcaatt gccggtaatg ccattgcgat agatatggcg caggtcaaca
    25741 aacaaacctg ttttattaat atggcgacag gcggatttgg gacgcgtatt accacagaaa
    25801 cgccggaaaa attaaaagcc gcgctgggtg gcgtctctta catcattcat ggcttaatgc
    25861 gcatggatac tctgcaaccg gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag
    25921 gtgacgccct ggtcattggt attggtaacg ggcgtcaggc cggtggcggt caacaattgt
    25981 gcccgaacgc gttaattaac gatggcttgc tgcaactgcg catttttacc ggcgatgaaa
    26041 ttcttccggc tctcgtatca accttaaaat ctgacgaaga taacccgaat attatcgaag
    26101 gcgcttcgtc gtggtttgat atacaagccc cacacgaaat cacttttaat cttgatggcg
    26161 aaccgttgag tgggcaaaac ttccatattg aaatacttcc ggcggcgttg cgttgtcgat
    26221 taccaccaga ttgtccatta ttgcgttaat caaactattc tctcttgaac cagggagaat
    26281 ccgtaatgcc ggatgcggtg taaacacctt atccggcata cagaacaata ctacaccgta
    26341 attaatttta cccgtgtctt ctgcaatgcc aatttatcca cctctgacac agcatcatcg
    26401 gtaacaataa tgtcaatttt ttcgattggc agtacctgat taaaaccgcg acgattaaat
    26461 ttcgatgaat cgagaacggc aatcacttta tttgcggcgg tgaccatcgc cccgctaatc
    26521 gaataacctt cattaaaggt cgtaatacca ttaaccgcat caataccatc agcaccgaca
    26581 aacattaaat cggcattaat atcctgcaat gaacgctcgg caatggaacc atgcatcgag
    26641 cgcgttttat ggcggaccgt gccaccacag acgaccagag taatgtcttt attttcggaa
    26701 agggcaaacg ctgccgggag actgttggta atcaccgtga tatttttagc ggtcattaat
    26761 ccttcggcga taagcattgt ggtacttccg ctgtcgagaa taacggtcat cccttcatgg
    26821 atcattttga ctgccgcctg cgctattctg ttttttggcg cgctggcgag ctgaaatcgc
    26881 tctttaaacc cgacttcctg ggtctcggtt tcactattac cggacattat tttcgccgca
    26941 ccgccatgaa agcgcgtaac cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt
    27001 gtcgcttccg aggcagcaaa tactcccgcc agatcctgaa caagcacggt tccctgttca
    27061 ttcactaatt ggatgatctt attccttcgc tcgaatgaat tcatcgtcat ttacccatat
    27121 tcaattgtgg ctagtgtaaa cgaaggggga ccattggtga atgatctgtt ttgcacaata
    27181 ccgggccagc atcacgctgg cccgcggttt catggaatga gcaacacttt gccctgcatg
    27241 gcattacgag cgatgtcacg caccgcctgg gtgaagcttt caaagcttcc acggtgagcg
    27301 attaatggct ccaggcttaa cttacgttct gtcagcaacc ggctcgccgt ttcccactcc
    27361 tgccccggcc aagggctgga atagttcatc caactgccga taaccgtcag ctctttacgc
    27421 aatattttgc caaacgttgc cgatgttaaa tgcagatcct gatgcaacgt gcccaccagc
    27481 gccagttggg catgaggacc ggcaatctct accgccagtt cgacagtttg cggtacgcca
    27541 gccgtctcga ggataagctg attaaagcgc agttcgcgta aaacgctctg catttgcggc
    27601 gcgctcattt cgctactgtt aaatgtttgc atcgcaccga aagattttgc cagtgccagt
    27661 ttttctgagc taatgtcgat cgccgtcaca ctctttgctc ccagcgcgac agcgcactga
    27721 atagccagca ggccaatggt tccggcacca ataataataa cgtttttatt ctcacaacct
    27781 tgcgctaaat gaaaagcatg caggccaacg gtaatcggct caataaaagc cccatcctca
    27841 ataggcatat ccgtgggtag agcaaagaca ttttttcgct taacgacaat atattcagca
    27901 aatccaccat c
//
LOCUS       DAJVPT010000049        27861 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVPT010000049 DAJVPT010000000
VERSION     DAJVPT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..510
                     /locus_tag="OQM47_002055"
     CDS             <1..510
                     /locus_tag="OQM47_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480215.1"
                     /translation="TIWEKAIFMRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALP
                     FDVRIISASQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTL
                     QVNIPHEAGHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPET
                     KFTLLNVTK"
     gene            507..893
                     /locus_tag="OQM47_002056"
     CDS             507..893
                     /locus_tag="OQM47_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480216.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            918..3131
                     /gene="tssI"
                     /locus_tag="OQM47_002057"
     CDS             918..3131
                     /gene="tssI"
                     /locus_tag="OQM47_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1480217.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3154..3598
                     /locus_tag="OQM47_002058"
                     /pseudo
     CDS             3154..3598
                     /locus_tag="OQM47_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3620..4027
                     /locus_tag="OQM47_002059"
     CDS             3620..4027
                     /locus_tag="OQM47_002059"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCS1480218.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4024..6312
                     /locus_tag="OQM47_002060"
     CDS             4024..6312
                     /locus_tag="OQM47_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1480219.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6309..7298
                     /locus_tag="OQM47_002061"
     CDS             6309..7298
                     /locus_tag="OQM47_002061"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480220.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7398..7601
                     /locus_tag="OQM47_002062"
     CDS             7398..7601
                     /locus_tag="OQM47_002062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480221.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7619..7939
                     /locus_tag="OQM47_002063"
     CDS             7619..7939
                     /locus_tag="OQM47_002063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480222.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8005..8237)
                     /locus_tag="OQM47_002064"
                     /pseudo
     CDS             complement(8005..8237)
                     /locus_tag="OQM47_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8347..9117)
                     /gene="yafV"
                     /locus_tag="OQM47_002065"
     CDS             complement(8347..9117)
                     /gene="yafV"
                     /locus_tag="OQM47_002065"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1480223.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9298..9744
                     /gene="ivy"
                     /locus_tag="OQM47_002066"
     CDS             9298..9744
                     /gene="ivy"
                     /locus_tag="OQM47_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1480224.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9787..12231)
                     /gene="fadE"
                     /locus_tag="OQM47_002067"
     CDS             complement(9787..12231)
                     /gene="fadE"
                     /locus_tag="OQM47_002067"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1480225.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12471..13049
                     /gene="lpcA"
                     /locus_tag="OQM47_002068"
     CDS             12471..13049
                     /gene="lpcA"
                     /locus_tag="OQM47_002068"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1480226.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13255..14022
                     /locus_tag="OQM47_002069"
     CDS             13255..14022
                     /locus_tag="OQM47_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1480227.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13993..14733)
                     /locus_tag="OQM47_002070"
     CDS             complement(13993..14733)
                     /locus_tag="OQM47_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1480228.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15117..15638
                     /locus_tag="OQM47_002071"
                     /pseudo
     CDS             15117..15638
                     /locus_tag="OQM47_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15814..16311
                     /gene="rayT"
                     /locus_tag="OQM47_002072"
     CDS             15814..16311
                     /gene="rayT"
                     /locus_tag="OQM47_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1480229.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16528..18240)
                     /locus_tag="OQM47_002073"
                     /pseudo
     CDS             complement(16528..18240)
                     /locus_tag="OQM47_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18212..18997
                     /gene="lafU"
                     /locus_tag="OQM47_002074"
     CDS             18212..18997
                     /gene="lafU"
                     /locus_tag="OQM47_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1480230.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19068..20123
                     /gene="dinB"
                     /locus_tag="OQM47_002075"
     CDS             19068..20123
                     /gene="dinB"
                     /locus_tag="OQM47_002075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1480231.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20175..20468
                     /gene="yafN"
                     /locus_tag="OQM47_002076"
     CDS             20175..20468
                     /gene="yafN"
                     /locus_tag="OQM47_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1480232.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20471..20869
                     /gene="yafO"
                     /locus_tag="OQM47_002077"
     CDS             20471..20869
                     /gene="yafO"
                     /locus_tag="OQM47_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1480233.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20879..21331
                     /locus_tag="OQM47_002078"
     CDS             20879..21331
                     /locus_tag="OQM47_002078"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1480234.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21577..22620
                     /locus_tag="OQM47_002079"
     CDS             21577..22620
                     /locus_tag="OQM47_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1480235.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22682..23296
                     /locus_tag="OQM47_002080"
                     /pseudo
     CDS             22682..23296
                     /locus_tag="OQM47_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23353..24810)
                     /gene="pepD"
                     /locus_tag="OQM47_002081"
     CDS             complement(23353..24810)
                     /gene="pepD"
                     /locus_tag="OQM47_002081"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1480236.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25072..25530
                     /gene="gpt"
                     /locus_tag="OQM47_002082"
     CDS             25072..25530
                     /gene="gpt"
                     /locus_tag="OQM47_002082"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1480237.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25622..26866
                     /gene="frsA"
                     /locus_tag="OQM47_002083"
     CDS             25622..26866
                     /gene="frsA"
                     /locus_tag="OQM47_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1480238.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26924..27325
                     /gene="crl"
                     /locus_tag="OQM47_002084"
     CDS             26924..27325
                     /gene="crl"
                     /locus_tag="OQM47_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1480239.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27364..>27861)
                     /locus_tag="OQM47_002085"
     CDS             complement(27364..>27861)
                     /locus_tag="OQM47_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1480240.1"
                     /translation="DGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEA
                     WATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVL
                     SKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVG
                     MTYQF"
BASE COUNT         6857 a         6945 c         7443 g         6616 t
ORIGIN      
        1 actatatggg aaaaagctat ttttatgcga gcgatatatt tatctgttca gcaggcatgg
       61 aatgggaaaa taacttattc tgtttccggt gaatctgagt tcgctaaaaa atttcagggt
      121 aaagctttgc cttttgatgt aagaataatt tctgcatctc agaatgaaga ctggttggtt
      181 attgcaacaa aggttttgcc tggtgctgat ttaagaacat atgttgattt taaaaacagt
      241 acggttcatg ttgattcagc gggcctggaa aaagtagcga aatgtattaa ctgtaataat
      301 acgttgcaag taaatatacc tcatgaagcc ggacatgtac ttggttactt agatgatgat
      361 tatgattctt cgtctccgta tgttggagat attagtgggt tgatgaatgt gggaatggag
      421 ctttgggaac gataccttaa aaacgcaact ataacgttga atatcataat gcctgaaact
      481 aaatttactt tattgaatgt aaccaaatga aatttttctg tattatagtg attttccttt
      541 caataaatta catagctata gtgaatgttt tcaagtcttc tgcagttgct caatccacca
      601 aatattatga agaaaatgat atacatgatg gaagtaatga catctttttt atgaagaaat
      661 atgggccgcc tgctcatgag ttcgtgaggc cacgatataa atttgttggt gagtattata
      721 tcggcttgaa tcaaacttat cgcataacag atcctgaatt tcaagatgtt cctattaagg
      781 aaatgttctg gaatttgcag ggggatttga atctgacatg ttggtttcat tataaagacg
      841 ggcaatggag agtgattact tatatttttt ggccaccagg ggctagattt taatccggta
      901 ttaaaggagc aaatatcatg agttttgtat ctacgggtaa taaaccagtt ggaaacggtg
      961 gacccgtcat caccacacca ccaatagctg gcgaaagtgg cggtatgagc acaggttcag
     1021 ctgtaaccga tgtatctggt gccgccgagg agatggcaga acaggctgcg gcggacctgt
     1081 ttggtgcgct gccggaaccg tcaggtctgg ttaaagccgc agtagctgct gcgcaggctg
     1141 ccgctgcagc agcagggatc tcggatatgg ctggtgcagt tcaggatgca gcggcgtcgc
     1201 ttgcagccgg tgctccgggg gcgcataacg tcacagtaag tggtagtgcg gtgccgccgc
     1261 agatgctttt gtttgcaggc atgaatggtt cggaagggct tgggaatctg ttttcttaca
     1321 cagtacagct taaaacgctg gacgccctga acctggggta cgtttccccc gctgctaacc
     1381 tgccgcttca gctcatggtg ggcaaagacc tgtgcgtcag tatcgaactg gacggcggcg
     1441 gaaaacggta tatcagcggg ctggtgacgg cggcgagggt ggtcggccat gagagccgtt
     1501 cggtaacgta tgaattacgc attgagccat ggctgaagct gctgacccat accagcgact
     1561 acaaagcgtt ccagaacaaa accgtggtgg atattctcga tgaagtactg gatgaatatt
     1621 cctttccggt ggaaaagcgg ctggtggaaa attatccacc ccgcgcctgg caggtgcagt
     1681 atggtgaaac cgacttcgat ttcatccagc gcctgatgca ggaatggggg atttactggt
     1741 ggtttgagca cagcgagaac agccacacgc tggtgctggt ggatgccatc aatggtcata
     1801 aagcgtgccc tgactcgccg ctggtggagt ggcaccagga agggctgaaa ctggataagg
     1861 aatttatcca caccattacg gccagtgaga ggctgcgtac tggaaagtgg gtgatggacg
     1921 attttgattt tatgaagcct cgttcgctgc ttaaaagtac cgtggccagt ccgcgtgata
     1981 ccggtcatgc tgaatacgag cattacgaat ggcctggtga ttacttcact acgggcgaag
     2041 gcgagatgct gacgcgcatt cgtatggaag cgcagcgcag cccaggcagc cgggcacatg
     2101 gggccggtca tatccgtacc ctcatgaccg ggtacacctt tacgttgatg aatcacccga
     2161 cggcagaaat taatcaggaa tatctgctgg tacagaccac tctgttttta cgggataatg
     2221 cgcagcacag cggtcaggat cagcatttta cctatgtcac cacgtttgaa ctgcacccca
     2281 ccagcgaagt ttaccgccca caacgaaccc tgagcaaacc ccacaccaaa ggcccgcaga
     2341 gcgccatcgt caccggcccg gcggggcagg aaatctggac ggataaatat ggccgggtga
     2401 aggtgcagtt tggctgggac cgttacggaa agaatgacga aaacagttcc tgctggatcc
     2461 gcgtcagcta tccgtgggcg ggtaaaggat tcggcatgat ccagatcccg cgtattggcc
     2521 aggaagtact ggtggatttc aaaaacggcg atccggattt gcccatcatc gtggggcgca
     2581 cctataacca ggacaccatg ccgccgtggg ggctgccggg catggcgtcg cagagtggga
     2641 tattcagcca ctcattgcag ggagggccga ctaacggaaa tatgctgcgc ttcgatgata
     2701 aaacgggtgc ggaagaggtg aagttccatg cggaaaaaga cctcaatacc acggtaaaaa
     2761 acaatgaaac ccacacggtc atggtcgatc gcataaaaac cattgtcaaa aatgaaacca
     2821 tcagcgtggg agaagaccgt aatgccacca tcacaaaaaa tgatggtctt tccgtcaaac
     2881 tggctcaaac catcaatgtc ggaacgacat atcgtctgga tgttggcgat caatttaccc
     2941 ttcgttgcgg caatgcagcg cttgtattac acaaggatgg ctcgattgag ttttgcggaa
     3001 aacaactgtt actgcacacc agcgatgtta tgcaactcat tggcagtggc attgatatga
     3061 atccggatgg cggcacagcc atgactgcgg atgatattgc cccgttacct acccccgacg
     3121 tttctgaata aaaaatataa aaggaatttt tctatgacca gcctttaccg tattcaggag
     3181 ggctgctttg ccctgcctga aacttttctt gatcgcaccg tcaatatctt cgtcccatcg
     3241 ggcaatgaaa gggcaactcc cagtctgaat attttccgcg atacgctcag gccagatgaa
     3301 aatctgacca cctatattga ccgccagata gccttgatga aaaaaaaacc taggccagca
     3361 ccgggtactt tcgcggggaa cggcactggc gggaactggc gaagctgcca tgacaggtga
     3421 acagattctc gcaactcatc gttctggaaa aacagaagtg taccagcgtc aggccgggtt
     3481 tatcaccggg ccagcaaagg tactaatgtt gacgctgacc actcagcgtc cttttgatga
     3541 tcacaccgac cagctatgga ctgcatggtt gactagtttt cagcctgcta aaagctaaaa
     3601 aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg gatgacccca tctaccacac
     3661 cagcgccctt gccgggtttt tgataggggc catcataggt atcgccatta ttgccgtcgc
     3721 cagctttgct ttttttacct gcggcttttt ggccgggctg attttgagtt ttctggctga
     3781 tcagatagct tccggagtcc tgcaactggg tgaggctatt gggcgctcga tacattccac
     3841 agtgggaaaa attctgaccg gttctgataa tgtcagcacc aacagtcgcc cggccgcccg
     3901 ggccgtactg agtacggtga tatgcgaaga ccattccccg gaaatccgca ttgcccaagg
     3961 ttccggcaac atctacatca acagccagct gcccgtaagg atgaccacac tgagtgtgat
     4021 gcagtgattg aggacggctc ccccaatgtc tttctcggcg gcggtacgca gacggtactg
     4081 gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg tggatgtgct gtatgtggtc
     4141 gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg cggcaaagat ggggtcgaaa
     4201 tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa tggtcgggat ggccatctct
     4261 gacacagtca tgggattgtt cagcaatccg gtagatgtca ccactgggca aaaaattttg
     4321 ctgccggaga cggattttac cctgccagga cgcctgcctg tcacctgttc gcgcttttac
     4381 gccagccacc tggaaactga aggattgctg ggcagaggat ggcggctgaa ctgggagata
     4441 aacctgcgtg aggatgaaac atatatcacc ttcatcggtg tacagggtcg ggaactgagt
     4501 tatccgaaag agatgctgat accgggccac cagatattcg acccggaaga gcagttttac
     4561 ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca ccgactgcag ctattacgtg
     4621 tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt ttatggagac gccgtaccgg
     4681 cagcgtattg ctttcgggcg tgagaacggc aggctggtgc gggtggcctc cagcagcgga
     4741 caccatctgt tattgcaccg cacgatgacg caagcagggg aacgcctgtc gcatattgaa
     4801 cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc gttacgacga taacgggcaa
     4861 ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc agtttgccta tgaaaatggc
     4921 ctgatgacag agcatcgcaa cgcgacgggc tttacctgta cctaccactg ggaagagatt
     4981 gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg gcgaggatta ccggttccac
     5041 tatgattttg ctggcgggca gacggtggta accggcaggc cggagcagaa gtggcagtgg
     5101 tggtttgatg aagagacgta tgtgactgcg caccggacgc cgggcggcgg aatgtaccgc
     5161 ttcacgtaca acgaaaacca ttttccggta gcggtggaac tccccggcga acgcagggta
     5221 acgctggaat acgacaccct gagccgggtg gtgaaagaga cggacccggc aggccgggtg
     5281 acacagacgc agtggaacgg cagttttgct gaaatcacgc gccgggcgct ggatgacgac
     5341 cacgtatgga aggcggatta caacgaacac ggccaggtga tacgggagac ggacccggaa
     5401 gggcgcgtca cgcggtacgg gtatgatgat caggggctgc cggagacggt ctgccacccc
     5461 ggtaaacagc aggaccgtta cacctggaac gcgctggggt tactgagcag ccaccgtcgc
     5521 atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg ggatgctggc gcgccatacg
     5581 gatgaagaga aacgggagac gcgctggcag tacaccccgg aagggctggt tgccagtctg
     5641 agcaacggca acggggctca gtaccggttc agctacgacg gcgacggacg gctgacggga
     5701 gaacagcgcc cggacggcct gataaggatg tttgcgctga atgcggacgg gtttccggtc
     5761 atcatcccga cgcagggcac agagggcggg gtgcgcaatg aacagcagga gcgtgatgcg
     5821 ctgggccgcc tgctgcgcag tgacacgcag cacagtacac ggaccttcag ctacaaccgt
     5881 cttgaccaga taacggaagt gacgctcacc cccacggaag aaggggaacg gctgcaccac
     5941 atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg ggtggctgac ggcagagcac
     6001 agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg gcaatcccac cgatatcacg
     6061 ttgccggacg ggcagcatct cagtcatctt tactacggca gcgggcatct gctccagacg
     6121 gcactggacg gtatcacggt cagtgaatat gagcgtgaca gcctgcaccg tcaggtgata
     6181 cgcacgcagg ggaaactggc gacgttcagc ggctacaacg ccgacaaccg gttaagctgg
     6241 cagcgcagcc tgccaggtgg cagccagaat ccacagcagg cggtactccc ccggcgcaga
     6301 acgacagcgt gacggcgcgg gactatatct ggaacgcaga cggtgaagtc ggcggcatca
     6361 atgacaaact gcgcggttgc ctggtgttca gctatgaccg cagcggctgg ctgaccagcc
     6421 gcacggggca gatgtatgac catgaccatt attattacga caaagcgggc aacctgctga
     6481 cggatgagta tcagggagcg gtgatggata accgcctgcc gggttacggg cgtgaccgct
     6541 accgctataa cgaatggggc gaactgacag agcgcaggga ccagcagctg gagtggaacg
     6601 cgcagggcca gttaacgcgg gtcatcagca gcaacagcga gacgcggtat cagtacgatg
     6661 cgctgggcag gcgcatcagc aaggcgacca gcaatctcca taccgaccgg ggagaacgaa
     6721 gccggacaac gtttgtgtgg gaagggttcc ggctgttaca ggagacaacg tggcagggaa
     6781 agcggacata cctgtacgat gcggaacagc cgtacacgcc ggtggctgcc atcacggggc
     6841 gcggagagag ccagaaaatc tggtattacc acacggacct gacgggcacg gtacacgagg
     6901 taaccgcgcc ggacgggacg ctggtgtggg cgggctacca ggcgggattt ggtgaaaacc
     6961 ggggcgacat cagcaacagc ggggcgtact ttgagcagcc gcagcggctg ccggggcagt
     7021 actttgacga agaaacaggg ctgcattaca atctgttcag gtattatgcg ccggagtgcg
     7081 ggaggttcgt aagccaggac ccgataggat taaacggtgg gttgaactta tatgcttatg
     7141 cgcctaatcc gttaggttgg atcgatccgc taggactgaa ttctttagga atagattcaa
     7201 ataatatatt tagaggagat ggtaactata aaggtggcgg aattggacag ccaaagagtg
     7261 gcacaatatc agcacaggac attattgatc atctctaaga aaaaggtgat gggcgtttaa
     7321 catcttttag tactaaaaaa acatctagtt ccaactctcg tggtgcagat tttttctcaa
     7381 aaaaccatgt cttcaaagtg gcagtttctg atctcgatga tttagttaaa aataatcaga
     7441 tcactgtttt aacacaagaa aaagtaaaag caatatttgg tttagaatca agcaagaaaa
     7501 ttagaaaaga acttggtaat attcttcaga atatggctag gaataatgag gtgttagttc
     7561 atgggcaaat tcttggaaat aaagttagcc gatgtaatta gttagtggag aggaagttgt
     7621 ggacgaaatt agaataagaa ttgctggtaa tacggctgcg ccttgtttcc atgtgctaaa
     7681 aaataaggga tataaagtaa caatggaaac tcatgcatta tctgacgatt gtttagattg
     7741 tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt agtgcaacga gtccagaaga
     7801 gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat gactggagag ctaatttgaa
     7861 tgagataaat acttatcaaa aagccataga agaatccccg ttttttgata aaaacggcaa
     7921 tgaaatagag tctgactaaa ttttattgat atagcaacga taataaccgg aaatttcttc
     7981 cagatctttc cggcttttta ttatttaact ttcgttcagc acactaaaca tcccgtctcg
     8041 catcccctta actggctgtt taacctgata aagttccctg cgcagttcct gtacttcgtt
     8101 gttatcccaa gcaggatcaa acatccctcc gagatcttca cggtaacgcc cgtgccgatc
     8161 tcaaatcccg cttttctcag ccatttggcg ctgattttga tcaccggcgt gtcgctgcgg
     8221 ttgggcagat aacccacggt acagcgacgc tgtgttttgg aaatggtgac ttctggcgta
     8281 atattgtgtt taagttaggg gctttcgtaa tctagcccag cccccggccc taaacacaac
     8341 ccccactcac cacaacctaa actcatccgc atcccgccat gccggaaact tttctctata
     8401 ttcccgcaat gccaccatcg acagctccgc atcaatacgc gttgccttat gcgcgtcggc
     8461 ggtagcgata atctcgcctt gcggattaat cacccggctg tcaccgcgat aatggcagcc
     8521 gttgccatcg ctgccgacgc gattgcatcc cgccacatac gcctgattct caatcgcgcg
     8581 ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct ggccagttgg cgacgtacag
     8641 ggcgaggtca taatcgttga gattgcgcga ccacactgga aaacgtaagt cgtagcacac
     8701 cagcggcaaa atacgccagc cgcgccattc cacaatcact cgcgcattgc ccgctttata
     8761 atgcagatgc tcatctgcca tgcggaacag atgacgctta tcataaaaat gtaccgtgcc
     8821 gcccggctca accagcaaaa agcggttaac cgaaccagac tccgtttgta atgcaacact
     8881 gcctgcaatc agcgcattgc actgctgcgc cttagctgtc atccagttca ctacgtcatc
     8941 ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg ctggtaaaca tctccggtag
     9001 aacgatcaca tcgcgcccgg taataccttc cagttgacga tcaaaatggc gcaggttggc
     9061 aggaccatcc acccacacca acggttgctg caaaagcgta atcttcaaac taggcacggt
     9121 gtacaactcc tttatgcgaa gggttttata actttaacac cttatcaggc agttgcctta
     9181 gcgcagaata aattgataac aaatgctgat attggaaata tctgatttgc aaattatcgt
     9241 gttatcgcca ggctttagga ggttaataac atgggcagga taagctcggg aggaatgatg
     9301 tttaaggcaa taacgacagt cgccgcactg gtcatcgcca ccagtgcaat ggcgcaggat
     9361 gatttaacca ttagcagcct tgcaaagggc gaaaccacca aagctgcgtt taatcagatg
     9421 gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg gtacttatac tcccgcacaa
     9481 accgtaacgt tgggagatga gacgtatcag gtgatgagcg cgtgcaaacc gcatgactgt
     9541 ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta atcagatgac ggggctgttc
     9601 tcggctattg atgagaaaac gtcgcaagag aaactcacct ggctgaatgt gaacgatgcg
     9661 ctttcgattg atggtaaaac ggtgctgttc gcggcgttga ccggcagcct ggaaaaccat
     9721 ccggatggct ttaattttaa ataattagcg gataaagaaa cggagccttt cggctccgtt
     9781 attcaattac gcggcttcaa ctttccgcac tttctccggc aactttaccg gcttcgtcgc
     9841 cagctcttcc ggatcaaagt catcaacgtt aatactgcgc agacggcttt cttccgcttt
     9901 caccagaata gcggcttcat ctttatcaat cagccccttc gtcagcgcgt tgtgcgccag
     9961 ttcatccaga cgggtaaatg gcagattttt acccagctct ttacagatcc gctgatgaat
    10021 cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt aaaccaaccg ggttatgctc
    10081 gcttggcgtc aggtactgac cgcgaccaat acgtgaacga gtggcgttcg gcacttgtaa
    10141 aatcttcgcc attttatgat ccagcttgtc agacggtgcc agataatgac gtccggtcgg
    10201 gaagatcacc acattcagca gcccggcaac cacgcggttc gggaagtttt gcagtaaatt
    10261 gtccattgcc tgttctgcct gatacagcgc atcctgtacg ccccagtgca ccagcggcag
    10321 atcggcttca ttacggcctt cgtcgtcata acgcttcaga acggcagagg cgaggtaaag
    10381 ctggcttaaa atatccccca gacgtgccga gatacgctca cgacgtttca ggctgccgcc
    10441 cagcagtgcc atcgagacat cagaaagcag ggcgaggttg gcgctcaggc ggttcaggtg
    10501 ctgatagtag cgtttagtgg catcgccggt tggcgtgctg ctggttaaac cgcgcgtcag
    10561 gcccagccag aagctgcgaa ctttgttgct accgacgtga ccgatatgtt tgaacaacag
    10621 tttatcgaac gcgttgacgt cattgttctt cgccgcttcc atctcttcca gcacgtacgg
    10681 atggcaacga atcgctcctt gtccgaagat catcatgctg cgggtcagaa tgttagcccc
    10741 ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc gccaggaagt tgctttgccc
    10801 gagcataatg cctttaccgc cggtaatatc catcgcatca ataatcgact gctgcccgcg
    10861 gtgggtacag tgatacttaa cgatagccga cagcacggca ggtttttcgc cgagcataat
    10921 gccgtaggta atcagcgatg ccgcagcatc cattacataa gcgttcccgg caatacgcgc
    10981 cagcggctct tcaatccctt ccatcttacc gatagagatt ttgaactgac ggcgaatgtg
    11041 agaatacgcg cccgttgcca gcgctaccga tttcacgccg ccggttgagt tggaaggcag
    11101 ggtgatgccg cggcctaccg agaggcactc caccagcatc cgccagcctt gcccggccat
    11161 tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca tctttaccgc gcgtcggtcc
    11221 gttctggaac ggtacgttca gcgggaagtg gcgacgacca atttccacgc ccggcgtggt
    11281 ggttgggatc agcgcacagg taatgcctaa atcttctgca ccgccgagta atttttccgg
    11341 gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc ggtgccagcg taatgtagcg
    11401 tttgttccag gtcagacgca tccccagcac ctgctggccc tgccattcgc ccatgcagac
    11461 aatcccggtg tccggaatcg cgcccgcatc ggaacccgct tccgggctgg tcagtgcaaa
    11521 gcaggggatc tcctgaccac gcgccagacg cggcagatag tgatctttct gctcgtcagt
    11581 gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt ggcacgccga cggtaatcgc
    11641 caggatcccg ctcacgccag agagtttttg cagcacacga gactgggcat aagctgagaa
    11701 ctccagtccg ccgtactctt ttttgataat catcgcgaag aagcgatgct ctttaaggta
    11761 cgcccacaac tccggtggca gatccgccag ctcatgagta atctggaaat cattcgccat
    11821 ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc tcttcagcgg tcaggcgcgg
    11881 ctgcggatag ttatgcagct ttttccagtc cggcttgccc tggaacaagt cgccctccca
    11941 ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc gacatcggcg gcatcacctt
    12001 acggaaaccg cgaaataccg gcgcggaaat catcgactta cgcataggcg caaagttaaa
    12061 tggcacgagg ataatggtca gaggcaccag tacccacgcc gaccacagac cagcaacgcc
    12121 gagggcggct gtccaggcga gcaaaatcag actgctgata aataagctca cgcggtgata
    12181 gaacaacgcg ccgagcagga caaccgtagc gagaatactc aaaatcatca taacgaaaag
    12241 ccccttactt gtaggaggtc tgaccacttg tgatgatatg gttgtagtgg atgtaaaaac
    12301 atttagcaat gtgtttacaa tataattaca acaaagctca cattgttgct gtttttatcc
    12361 gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg tacttctcgc ttttggcggt
    12421 atccggtaca ctgcattttg tctattacat ttatgctgaa ggatatcctc atgtaccagg
    12481 atcttattcg taacgaactg aacgaagcgg cggaaacgct ggctaacttt ttaaaagatg
    12541 acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt agcagacagc tttaaagccg
    12601 gtggcaaagt gctttcctgc ggcaacggcg gttcccattg cgacgctatg cactttgccg
    12661 aagagttgac cggtcgctac cgtgaaaacc gtccgggcta cccggcgatt gctatttctg
    12721 acgttagtca tatttcctgc gtcggtaatg atttcggttt caatgatatt ttctcccgct
    12781 acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg gatctccacc tccggtaact
    12841 ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa ggggatgaaa gtgatcaccc
    12901 tgaccggtaa agacggcggt aaaatggctg gcacggcgga tatcgaaatt cgcgttccgc
    12961 actttggtta tgccgaccgc attcaggaga ttcacattaa agtgatccat atcctgatcc
    13021 agttaattga aaaagagatg gttaagtaag tctggcgtag gccggataag gcgtttacgc
    13081 cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg acgcgtctta tcatgcctac
    13141 aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg ccgcatccga cctgaaaatt
    13201 cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt tgtggaggtt acccatgtgc
    13261 gaattgctcg ggatgagcgc caacgtccct accgatatct gctttagttt caccggactt
    13321 gtacagcgtg gtggtggaac cgggccacat aaagatggct ggggcattac cttttacgaa
    13381 ggtaaaggct gtcgcacatt taaagatcca caacccagct ttaattcccc catcgccaaa
    13441 cttgtccagg actacccgat aaaatcctgt tcggtggtgg ctcatattcg ccaggctaat
    13501 cggggcgagg tggcgctgga aaatactcac ccatttaccc gcgagttatg ggggcgtaac
    13561 tggacttatg cccataacgg acaactgacg ggctacaaat cactggaaac cggcaacttc
    13621 cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct ggctgctgca taaattaacg
    13681 cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt ttaaatatat cgcctcactg
    13741 gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc tttcggacgg gcgctatgta
    13801 atggcgtatt gttcgactaa tttacactgg atcacccgcc gcgcgccgtt tggcgtggca
    13861 acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc agaccacacc gaatgatgtg
    13921 gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa cctggcaaaa aattatgcca
    13981 ggcgaatggc gcttattttg cctcggggag cgtgtagttt gatgccagtt gtggctgcac
    14041 cacttcgtgg cttaacggct tgctgaccac gtaacggcca ttggccacag aaacggtcgg
    14101 tggcttacgg gtttgctcaa agtagtcgta gcccggcttc agctgctccc agaagtcctt
    14161 aaagttggaa tatttatggc gcttcatatt ggcgtcggtc atgcggaatg ggtaaatact
    14221 cacctgcacg ctcggctgac caaacaccag cgcaccagta acgaactgga agatctcatc
    14281 aataccctga ttggtcattg cgtagcagcc gatggataca caatcgccgt ggatcatcag
    14341 gtatttccct tcataaccat gcgcacggtc ataagcattg gggaatccaa tattaatcgc
    14401 tttgtagtaa cggctgtctg gttttaactg attacgctgg acgctataaa acccttccgg
    14461 gcttttgaaa tcgccctgac gctgttttgg ccctaagccg ccggaatatt tacagatttt
    14521 ataactgtcg agcagttgat attgctcgcc cattttgacg taaagatcga gcgtacgttc
    14581 ttccttgaag atctggatgt agacagggga tcccatcaac tgctgcttat actctttgct
    14641 cactggcgtg gtcgagctac tgctgcccag caaaccggca aacgaaacgc acgggatcaa
    14701 caacatcgca agaattaatg cgattttacg catactgctt attccttgat aaaacggtta
    14761 cacacgccag gacggcaaaa tggatcccaa atcggaatag tctggatttg gaaggctcac
    14821 attatcacca aaagagtttt acgcaagcct gtcggcgctg ggtttacaaa tttagcacag
    14881 gggcagtttt caatagtccg aaaagcgcat gtatcattta tttcctaata ttatgaaatc
    14941 tcaatagtaa tatgcaaata agcaattaat aatcccgttt actcctccgg tcttttaaaa
    15001 ttctcacctt cattcagccg gtttatgtct ttcatgtctt cgttcttgct cggcagattt
    15061 ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt tactcgccag cgccaccaca
    15121 accgggagca gataaaacag catccagaat ggtttcctgc gccgctcaag gcgagtgaca
    15181 gacgctggca ggcgctggcg gaaaacaacc actttttaag cagcgatcac ctgcataaca
    15241 tcaccgaagt ggcgattcac cgcctggagc agcagcttgg caagccttac gtctggggcg
    15301 gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt tttttatgcc tacaacaaga
    15361 tccttgaggc gaaactcccg cgcacggcca atgagatgta ccactaccgt cgggcaacga
    15421 ttgtggcgaa taacgacctg cgccggggag atttgctatt tttccatatc cacagccgcg
    15481 agattgccga ccatatgggg gtgtatctgg gcgacggtca gtttattgag tcactgcgca
    15541 ccggggaaac cattcgggta agccggttag ccgaaccttt ctggcaggac cattttttag
    15601 gcgcgcgcag gattttgacg gaagagatga ttttgtaggc cggataaggc gtttacgccg
    15661 catccggcgg ttgtgcacgg atgcctgatg cgacgctgac gcgtcttatc atgcctacaa
    15721 atctgtgcgt ttttgcgagc cgctttcccg atataacaca gcgtcattgt cggcctttcc
    15781 ttttcctctt accgttgaga aaaaggagtc gctatgtcgg aatatcgtcg ttattacatc
    15841 aaggggggaa catggttttt cacggtaaat ttacgcaatc gtcgaagcca tcttctgacc
    15901 acccagttcc agacgctccg taacgccatt attaacgtta agcgagacag accttttgaa
    15961 attaacgcct gggtcgtttt gccggagcat atgcattgca tatggacatt acccgaaagc
    16021 gatgatgatt tttcctctcg ctggcgggag attaaaaagc agttcaccca tgcttgcgga
    16081 ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca ttcgcaatac aaaagattat
    16141 cgccatcatg ttgattatat ttatataaat ccagagaaac atggctgggt aaagcaggtg
    16201 agtgattggc cattctcaac gttccatcgc gatgtcgcgc gagggttata tcccatcgat
    16261 tgggcgggag acataacgga tttaagtgcc ggggagcgta tcattttata actgtgcgct
    16321 ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt gctttaacgg taggccggat
    16381 aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc tgatgcgacg ctggcgcgtc
    16441 ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga taaggcgttt acgccgcatc
    16501 cggcaattgt gcaccgatgc cggattctta catcaacgcc cccataatct tcaactccag
    16561 ctcatccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca accttgcata
    16621 acgcggcagc aaagggcgta gctgcggcgg caccagcagc accggatctt tccccgccgc
    16681 tttcatctgc tctttcacct gtggcatcgt gctctggaac tggttgagca tattcgggtc
    16741 aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc gcctgattca ctacgttggt
    16801 cagcagattt tccagctcat tattcagcgt atacaccgtc agctcctgct tgcgaacgaa
    16861 cggatgagta atgctgcgcc gcagcgccag gcgcacatcg gccgccagca gaatatgatc
    16921 tttagtcacc gtgctactgg cgaccagcac ggtggcgatg gtgacgatat cgcgcaggga
    16981 aacgccttcg gtcagcagcg cccggtacac tttcagcaat tggctgtaat tgagcgccgc
    17041 gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa cggttatgca actgcgtgat
    17101 gtcatcatag ttaaacaaat caggaatata gctgcgcaca atcttgttca catgggtggc
    17161 aatcacactg gcgctgtcga tcacctgata ccccatattc agagccttcg ctttctgcgc
    17221 aggctggatc caggtgaccg gtatcccgta cgccgggtcg ttccccagca cgccgtcaat
    17281 ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa tatccgcttc
    17341 atcagcctca atgccgttga tgaaaatagc gtactggctg ggcttgaggc ggaagttttc
    17401 ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg gaaatcacct gccgaacgcc
    17461 gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc accagcgcca ccagcttgta
    17521 gccgaggctt aagctgatgg gctcgatcag cggaatagtt tcccaactga cctgttgttc
    17581 gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt ttctcttccg cctccgccgc
    17641 ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggcgctga acagcaaaaa
    17701 cggcagatgc ggcatccccg gcaccaccgc cagcacgaac atgatcccgg tggcggtgta
    17761 gagcaccgac gggcttgcca gcaactggtg gcgcacgtca tgggcgatat cgccgttgtc
    17821 gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc agcagggaag ggatctgcgc
    17881 caccaggccg tcgccgatgg tcatcagcac gtattgctgg aaggcggcgt cggcgctcag
    17941 gttgtatttg aagatgccga tacagacgcc gccgctcagg ttgatcgcca gaatcatcat
    18001 cccggcgatg gcgtctccgc gtacaaactt cgatgccccg tccatcgcgc cgtagaaatc
    18061 ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc gcctggttga tcaatccggc
    18121 gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc gcgtccaggg taaagcgggc
    18181 agaaacctcg gaaatacgct cggcctcttt ggtgacgacg ataaagttga ttgtcaacag
    18241 cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa
    18301 cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac
    18361 cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat gtgaatgaga aaacggcgct
    18421 gctgaagaag aaatccgcca ccgagcttgg cgagttggca accagtatca acaccattgc
    18481 ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg gattacgcgt
    18541 gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc
    18601 gttctttaaa actttgctgg tggagctcgc gccagtgttc gactcgctct ataacaaaat
    18661 tattattacc gggcataccg atgcgatggc ctacaaaaac aatatctaca acaactggaa
    18721 cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg gaagaggccg gaatgccgga
    18781 agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggacg cgaagaatcc
    18841 gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg accaaaagtg cgtccgatac
    18901 gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct
    18961 ggataagcag caggtgcttt cgcagcgaat gcgctaaatg ctgaatcttt acgcatttct
    19021 caaaacctga aatcactgta tactttacca gtgttgagag gtgagcaatg cgtaaaatca
    19081 ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgctctgc
    19141 gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca
    19201 attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc tactgggatg gcgctcaaat
    19261 tatgcccaca tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc
    19321 atatccgtga aatcttctcg cgctacacct cgcgcattga accgttgtca ctggatgagg
    19381 cttatctcga tgtcaccgat agcgtccatt gccacggttc tgcgaccctc atcgcccagg
    19441 aaatccgcca gacgatcttc aacgagctgc aactgacggc atctgcgggc gtggcaccag
    19501 taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta
    19561 cgccggcaga agttccggca tttttacaaa ccttaccact ggaaaaaatc cccggcgtcg
    19621 gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt gatgtacaaa
    19681 agtgtgatct ggtgattctg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc
    19741 gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttacgaaaa tccgtcggcg
    19801 tggaacgcac gatggcggaa gatattcatc actggtctga atgtgaagcg attatcgagc
    19861 ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta ctgattgctc
    19921 gccagggggt gaaattaaag ttcgacgatt ttcagcagac cacccaggag cacgtctggc
    19981 cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg
    20041 gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa
    20101 gacaactggt gctgggatta tgatgtatac tattatgtat attctggtgt gcattattat
    20161 gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc ggtcaatatc actgagttac
    20221 gtaaaaaccc agctaaatac tttattgatc aaccagttgc ggttctttct aataatcgcc
    20281 ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt aatggacatg cttgctgaac
    20341 aagaggagaa aaagcccata aaggcgcgct ttcgtccaag tgctgcaaga ttagaggaaa
    20401 ttacacgccg cgctaaacaa tatcttaatg atatgacgga tgatgatttc aatgacttta
    20461 aggaataagg atgcgggtat tcaaaacaaa acttattcgc ctgcaactta cagcagagga
    20521 acttgatgcg ttaacggcgg attttatttc ctataagcgt gacggtgttt tgccagatat
    20581 atttggtcgc gatgcactct acgacgactc gtttacctgg ccattaatca aatttgagcg
    20641 agttgctcat attcatctgg caaatgtgaa taatccattt ccgccacagt tgcgccaatt
    20701 cagcagaacg aatgacgaag cgcatttggt atattgtcag ggtgcgtttg atgagcaagc
    20761 atggttgctc attgccattc tgaagcctga acctcataaa ctggctcgag ataacaacca
    20821 aatgcataaa attggaaaaa tggcagaagc gtttcgcatg cgtttttgaa tttatattat
    20881 gaataacata caaataagag attatcagcc tggcgatttt cagcaactat gcgctatttt
    20941 cattagagcg gttacgatga ccgtcagtca gcattattca ctacaacaaa ttgccgcctg
    21001 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
    21061 agttattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt
    21121 atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa aaccttttat
    21181 taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg
    21241 ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt ggtttattaa
    21301 tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgc
    21361 ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct
    21421 ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc
    21481 gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc agcacatgca
    21541 acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat tcgtccctta
    21601 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
    21661 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
    21721 caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca
    21781 gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct
    21841 cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa
    21901 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
    21961 cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
    22021 aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat
    22081 agcggctcga gggggctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat
    22141 catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg
    22201 atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg
    22261 cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt
    22321 gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg
    22381 ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc tggcgcatgg ggctgggcgt
    22441 aaatgggggc gcaccgagtg taaagggcgt ctggcagcga aatacacagc gacgcaactc
    22501 tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata aacaactcat cttcgaagaa
    22561 gcgccacaag cctataaatc ggctgaaagt gtggtgcaat gtcagggcag gccggattaa
    22621 ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag
    22681 gatgatcttg ctataactct cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa
    22741 aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct
    22801 ggaaacagaa acaggccgct actctgacac gctgcgttcg gcgctgattt ctctggatgg
    22861 cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact attcagtgga tttgcccgag
    22921 cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg ggcattgggc gttttaccgc
    22981 tgatgagcag gagcaatccg atgcgatccg gtatgagacg ctgcgttcgt cggggccggg
    23041 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
    23101 tagcgtgaag gttcagtcag agcgcagcca gcatgctaac aaacggctgg cgcgattgct
    23161 gattgcctgg aagctggatc agcagcaata ggaaaatagc gcggtgctga aatcacagcg
    23221 gcgaatgttc catcaccaga ttgaacgtgg caacccgcga cgaacgttta cagggatggc
    23281 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
    23341 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta
    23401 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
    23461 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
    23521 cgcgtggata atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac
    23581 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
    23641 ggttttcgcg ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc
    23701 gctgtcgatt agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac
    23761 cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac
    23821 accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
    23881 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag
    23941 ctcgtttttc aggatctcct gataggtatt caccagagat ttcagtgcgt cgactttatc
    24001 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc
    24061 gttgaaatcg ataaggcgca ggtccagttc ttctgcatga cccgccagga agcgcaccag
    24121 cagtttgttg gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag
    24181 accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg
    24241 caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc
    24301 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
    24361 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt
    24421 ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt
    24481 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
    24541 gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac
    24601 cggtttacga ttttccatac ctgcggtagc aggttgacga atcaggatat tacctacctg
    24661 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
    24721 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
    24781 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
    24841 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
    24901 tatgtattga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
    24961 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
    25021 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
    25081 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
    25141 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggg
    25201 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
    25261 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
    25321 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg
    25381 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
    25441 gactatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc
    25501 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt
    25561 gttcttttta acttcaggca ggttacaata gtttccagta agtattctgg aggctgcatc
    25621 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
    25681 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga
    25741 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
    25801 tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg
    25861 taccgacgat gatttatacg ataccgtgat tggctaccgt ggtggcaact ggatttatga
    25921 gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt acggaagaag atccgcaact
    25981 cagtggtcga cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct
    26041 gaaaggagat gacctggccg agcaagcgca ggctttatca aaccgcgcct atgaagaggc
    26101 cgcccagcgt ctgccgggca cgatgcggca gatggagttt accgtacccg gcggtgcgcc
    26161 catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg
    26221 tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc
    26281 gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa
    26341 gctcacccag gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc
    26401 gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt
    26461 gcgtctggca taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt
    26521 tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt
    26581 tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa
    26641 tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc tgcccaacgc caatgttatc
    26701 aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc
    26761 atctgctgac ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa
    26821 aggtcttcag gaaatcaccg gctggatcga aaaacgcttg tgttaaaaat ttgctaaatt
    26881 ttgccaattt ggtaaaacag ttgcatcaca acaggagata gcaatgacgt taccgagtgg
    26941 acacccgaag agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg
    27001 taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc
    27061 ggcaccggaa gtgcgtgaat tctggggctg gtggatggag ctggaagcgc aggaatcccg
    27121 ttttacctac agctaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc
    27181 ggtaaaagat actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt ttcacgagaa
    27241 gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa ccggcgaatg attttcgtga
    27301 tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat
    27361 tcattaaaac tgatacgtca tcccaaccgc gacagtatca tcattattaa tattcagctt
    27421 attatcgcta tccagttggt tgattttata atcaacaaac gctgacatat ttttgttgaa
    27481 ataatacgta gcaccgacgt cgatataatt gaccagatct tcatcaccga tgccctcaat
    27541 atctttccct ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta
    27601 ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat
    27661 tttgcgtgtt tcagaataga aagttgccag ataaatatta ttggcatcgt atttcagacc
    27721 tgttgcccat gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt
    27781 gcgatctgag ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt
    27841 caatgacgtg ccgaagccat c
//
LOCUS       DAJVPT010000050        27810 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVPT010000050 DAJVPT010000000
VERSION     DAJVPT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..995)
                     /gene="yjfF"
                     /locus_tag="OQM47_002086"
                     /pseudo
     CDS             complement(1..995)
                     /gene="yjfF"
                     /locus_tag="OQM47_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(982..2004)
                     /locus_tag="OQM47_002087"
     CDS             complement(982..2004)
                     /locus_tag="OQM47_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1480241.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2018..3520)
                     /locus_tag="OQM47_002088"
     CDS             complement(2018..3520)
                     /locus_tag="OQM47_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1480242.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3660..4616)
                     /gene="ytfQ"
                     /locus_tag="OQM47_002089"
                     /pseudo
     CDS             complement(3660..4616)
                     /gene="ytfQ"
                     /locus_tag="OQM47_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4926..5456
                     /gene="ppa"
                     /locus_tag="OQM47_002090"
     CDS             4926..5456
                     /gene="ppa"
                     /locus_tag="OQM47_002090"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1480243.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5836..6177)
                     /locus_tag="OQM47_002091"
     CDS             complement(5836..6177)
                     /locus_tag="OQM47_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1480244.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6180..9959)
                     /gene="tamB"
                     /locus_tag="OQM47_002092"
     CDS             complement(6180..9959)
                     /gene="tamB"
                     /locus_tag="OQM47_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1480245.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9956..11689)
                     /gene="tamA"
                     /locus_tag="OQM47_002093"
     CDS             complement(9956..11689)
                     /gene="tamA"
                     /locus_tag="OQM47_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1480246.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11895..12533
                     /gene="msrA"
                     /locus_tag="OQM47_002094"
     CDS             11895..12533
                     /gene="msrA"
                     /locus_tag="OQM47_002094"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1480247.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12856..14199
                     /locus_tag="OQM47_002095"
     CDS             12856..14199
                     /locus_tag="OQM47_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1480248.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14261..14467)
                     /locus_tag="OQM47_002096"
     CDS             complement(14261..14467)
                     /locus_tag="OQM47_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1480249.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14792..15346
                     /locus_tag="OQM47_002097"
     CDS             14792..15346
                     /locus_tag="OQM47_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1480250.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15409..16347)
                     /locus_tag="OQM47_002098"
     CDS             complement(15409..16347)
                     /locus_tag="OQM47_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480251.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16559..17299)
                     /gene="cysQ"
                     /locus_tag="OQM47_002099"
     CDS             complement(16559..17299)
                     /gene="cysQ"
                     /locus_tag="OQM47_002099"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1480252.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17489..19432
                     /gene="cpdB"
                     /locus_tag="OQM47_002100"
     CDS             17489..19432
                     /gene="cpdB"
                     /locus_tag="OQM47_002100"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1480253.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19558..19938)
                     /locus_tag="OQM47_002101"
     CDS             complement(19558..19938)
                     /locus_tag="OQM47_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1480254.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20027..20887
                     /gene="qorB"
                     /locus_tag="OQM47_002102"
     CDS             20027..20887
                     /gene="qorB"
                     /locus_tag="OQM47_002102"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1480255.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20996..21961
                     /locus_tag="OQM47_002103"
     CDS             20996..21961
                     /locus_tag="OQM47_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1480256.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22069..22731
                     /gene="ytfE"
                     /locus_tag="OQM47_002104"
     CDS             22069..22731
                     /gene="ytfE"
                     /locus_tag="OQM47_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1480257.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22776..24188)
                     /gene="cycA"
                     /locus_tag="OQM47_002105"
                     /gene_synonym="dagA"
     CDS             complement(22776..24188)
                     /gene="cycA"
                     /locus_tag="OQM47_002105"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1480258.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24496..25116)
                     /gene="fklB"
                     /locus_tag="OQM47_002106"
     CDS             complement(24496..25116)
                     /gene="fklB"
                     /locus_tag="OQM47_002106"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1480259.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25335..25880
                     /locus_tag="OQM47_002107"
     CDS             25335..25880
                     /locus_tag="OQM47_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCS1480260.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25864..26511)
                     /locus_tag="OQM47_002108"
     CDS             complement(25864..26511)
                     /locus_tag="OQM47_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1480261.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26723..27514
                     /locus_tag="OQM47_002109"
     CDS             26723..27514
                     /locus_tag="OQM47_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCS1480262.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27524..>27810
                     /locus_tag="OQM47_002110"
     CDS             27524..>27810
                     /locus_tag="OQM47_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1480263.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDA"
BASE COUNT         6840 a         7415 c         6833 g         6722 t
ORIGIN      
        1 tatcgctgcg caatgttgac tcttgtcact ggcgaactct gacgattctc ccacagcacc
       61 gttaacccac gctgtaatgc tataaaaata aacaacaaaa taccgatggc gattttcgtc
      121 cactaggaac tcagcgtgcc gtcaaagttg atgtaagtct ggatcagtcc ctgaattgcc
      181 acgccaaaaa gcgtcccaag caccgtcccg acgccaccac ttaacaacgt accgccaatc
      241 accaccgacg cgatggcgtc cagttccaca cctacgcccg ccagtgcata tccggcttgg
      301 gtataaatcg agaagacaat ccccgccagt gttgccagtc cggtggagag catataaatg
      361 cgaatagtgg tgctgcgagt agaaatcccc atcaggttcg ccgacgtcgc gttgccgcca
      421 atagcgtaca cctgattgcc aaaacgggta caatgcgcaa ggaaaatacc gataaccacc
      481 accgccagca tcaacagccc catcgcacta aggcgaccgc cgccggggat tttccacgca
      541 aggcttgaga gcgtgtcata aatgggatgg tttatcggaa tcgactcttc cgaaacgaga
      601 tagctgacgc cgcgcaaaaa gaacatcccc gcaagcgtaa tgataaatgc cgggatcttc
      661 agggcgtcga tcagaagccc cataaatgcg ccgaaggcac agcccatcac cagcactagc
      721 ggaaacgcca gcagcggcga gaggccgaaa tcgccaacca cttttgccag aaacacgcca
      781 gtaaaggcga tcaccgaacc gacggagaga tcgataccgc cagagaggat cacaaaggtc
      841 atgccaacgg cgattatccc aagaaaggcg ttatcggtca ggatattgca gatcaccctt
      901 gtggaagcaa aaccgggaaa ctgggtcagg cagtagagat agcccagcac aaagacgccg
      961 atggtgatca tcagcggcaa gttacgtttt atcatggcta cgtactcctt taatcagact
     1021 gataaagcgt tgcgactgaa caatcagcac acaaagcacc accaccgctt tcaccacctg
     1081 gttcatctcc ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct gaataatcag
     1141 cgcccccact accgagagca gcaggttaaa gcgcccgccc atcagcgatc cgccgccaat
     1201 caccaccgcg agaatggcgt ccagctccag ccataatccg gcgttgttgg catcggcacc
     1261 gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc tcaacacgta
     1321 ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc gaatgttgat
     1381 accaacgact tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc agaacaggag
     1441 aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc cgctgccaaa
     1501 ccatgagaga tccggcgagt taaacgtgac gatctgcccg gcggtgatca gttgcgccac
     1561 gccgcgcccg gcgaccatca ggatcagggt ggcgacaaac ggctgaattt tgaggatcgc
     1621 taccagtatg ccgttccaca atcccgccag gatgccggtg cccagggcgc ttaacaaaac
     1681 aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg cgatagccat
     1741 caccgccccc acagagagat cgataccacc ggtggcgatc accagcgtaa taccaatcgc
     1801 cagtaacgca acgggagctg cacggttaag aatgtcgata gggctaccga acaaacgccc
     1861 atcctgaagc accacctgcc agaaatgcgg ggctaccagg ctatcgacca gcagcaccag
     1921 caacagtgcc gccagctgcg gcatccccgt cggccagtga aaacgccttt ttggcggcgt
     1981 agtgtcaggg agagattgag gcatcacact gttctcctta cgccgcaatg gcgttcatga
     2041 tcgccggaac ggaaagctgt gccagcggga tctccgccac ctgtttgcga tcgcgcatga
     2101 tgatcacccg gtcggcatag cccaccagct cttccagttc ggaggagatc accagcagcg
     2161 ccagaccgtc agcacacagc gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat
     2221 caatgccacg ggttagctca tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg
     2281 aaagcagcac tttttgctga ttgccaccgg agagaaattc aatcggttgt tcagttgaag
     2341 gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgc tctttgcggg
     2401 aaatgggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt tcccgcaccg
     2461 aggcggcggc gatgatgcca tcggttttcc tgtcttccgg gcagaatccg atgcccagta
     2521 ctgaagcctg atgtggcgat cgcagggttt gcggtttgcc tttgatcaac gccgtgccgc
     2581 tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtccg gatcccagca
     2641 agccagccag accgacgatc tcgccggggc gcacttcgag atcaaacggt gctatcgttc
     2701 cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg
     2761 cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc agttcgatct
     2821 gcggtagctc gcgcgtttcc cgacagccta cgaaactgcc gttgcgtaag acggtgatcc
     2881 gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaagatcagg ctgacgccgc
     2941 gatcgcgcaa ctgacgcatc aggccaaata atagctccat ttcctgggtg tcgaggctgg
     3001 cggtgggttc atcgaggatc agcactttgg cggagagatc gatagcccgg caaatggcga
     3061 cgatttgctg catcgccacc gaaaaacggt taagcggttc gcgcacgtcg agggagaaac
     3121 cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttacggcgt agaaggccga
     3181 aacgtttggg ttcgcgacct atgaatagat tatcagcaac cgacatattg ggtagcaggt
     3241 tgacttcctg atagacggtg ccgataccga gttgttgtgc atgggcggta tttttcggtg
     3301 agatagtctg gccttccagc cagatggtgc cgcgatcggc gtggtataca ccagttaatg
     3361 ctttgattag cgttgatttt cccgccccgt tttcaccaag cagcgccatg atttcgccac
     3421 ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc
     3481 cttcggtgcg gaggatctcc tggtgttggt cggtcgtcat ggtttccccc ggtgtaaagc
     3541 gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca gtgcaccatc
     3601 gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct
     3661 caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg caggtaaagg
     3721 gtggatttgg ttaacgtcag cttttcaggc atggtgccgt cttttttgta tttctccagc
     3781 gcatcgaaag cgggtcccgc catgttcggc gtcagttcaa cactggcgtt cgcttcgcca
     3841 tccatcatcg ctttgtagat gtccggtaca ccgtcaatgg aacccgtgag gatatctttg
     3901 cccggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat gtcgtcgtta
     3961 tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctctccatg
     4021 acttctttgc ctttactgcg ggtgaagtca cctgactgcg agcggataat tttgatattt
     4081 ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac gctggcccca
     4141 acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt taccagccag
     4201 tcaccgatca acttaccttc gaggatgttg tcggcagtaa cggtggtcat atagagagat
     4261 ttgtctttca catcgatgga acggtcgagc aagaagaccg ggatttcggc atctttcgcc
     4321 tcttttaata ccggttccca acccgtcgca accactggag cgataaagat cgcatccacc
     4381 ccttatgcaa cgaaggaacg taccgcttta atctggtttt cctgcttttg ctgaccatcg
     4441 gcaattttca acgtaattcc gcgtttttcg gcctcacttt tcgccacatt ggtttctgcg
     4501 gcgcgccagc cggattccga tccgacctgc gaaaatccta cggttaatgg agcggccaac
     4561 gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg
     4621 ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa
     4681 ttcgttatgt aacggattgc gttacatcac acttcaaaaa agtaaataaa acgttaatca
     4741 caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct
     4801 gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa caagcaaaga
     4861 cattcggcgc gagttggcta taatacttgg cacttgtttg ccacatattt ttaaaggaaa
     4921 cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg
     4981 ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa gagagcggcg
     5041 cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggttaca
     5101 tcaaccacac cctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc
     5161 cgctgcagcc tggttctgtg atccgttgcc gtccggttgc cgttctgaaa atgaccgacg
     5221 aagccggtga agatgcgaaa ctggttgcgg ttccgcacag caagctgagc aaagaatacg
     5281 atcacattaa agacgtaaac gatctgcctg aactgctgaa agcgcaaatc gctcacttct
     5341 tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg
     5401 cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgcaaagaat aaataagttc
     5461 ttctagcgca ataaccctga acgccgggct tcggttagta agggtttttt tatgcccgcg
     5521 ataaataaac tctctattcc accatcatta ttctcagcgg ttgcaaggct tgaacggtaa
     5581 gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa gttagccgac
     5641 ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc
     5701 cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga
     5761 tggaaaagtt ataaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaagga
     5821 ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa
     5881 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccatacggcg tctgaatcaa
     5941 ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
     6001 gtcaatacga taaacttcac cgtgtaccgt tccgttcccc ggaactgcgc ctggatagtg
     6061 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
     6121 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
     6181 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
     6241 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
     6301 acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag
     6361 tcgcctactc ccagggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
     6421 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
     6481 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
     6541 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
     6601 cgaactccgg cgattacatc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
     6661 agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac aatcagatcc
     6721 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
     6781 tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
     6841 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
     6901 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
     6961 gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg
     7021 agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc cattcgtacc
     7081 atcggcggca cggtgatccg cactttactg cctttcgccg ttactcgtgc ccgccagttt
     7141 tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact
     7201 gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct cggctgcata
     7261 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
     7321 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
     7381 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
     7441 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
     7501 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
     7561 agattcagtg tctgaaacgc caccggcagc gccgcatcgt tgacggtttg cgttacctgc
     7621 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt ggtgtcccag
     7681 gcaacatccg ctttacccgt gaagataccg ctggcctgag tggtttctgg cataaacggt
     7741 ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcccccgca
     7801 tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg tggcccgatg
     7861 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg
     7921 ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc ttctttgcga
     7981 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
     8041 agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
     8101 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
     8161 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
     8221 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
     8281 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
     8341 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg cccgagttcc
     8401 agatgaagcc cagggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
     8461 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
     8521 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactcc
     8581 gggaactctt tggcggtgtt aatgccatta agcgttagct caccgcgcca actaatggcc
     8641 tgctgccagt cgagcaacgc cttgagttca gttttccctt ccagcgccgc gacggtgagt
     8701 ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt ggctggcggg
     8761 atctcctgtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
     8821 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaagc
     8881 tgtttgctgt tcacttccac gttgagtggc aatccggctt ccgccagtcg cgtctgggcg
     8941 cgtaaatcca tatccaccgg accggaaagg ttaacgccta tctccagctg ttcgcgcagc
     9001 gcgccgccca ctttcagctt caccttttca cctttcaacg gctccacgtt cagtgtgctg
     9061 ttgagggtga tatccaccgg ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg
     9121 atcccctggt tggaatcgat atccagggcg tccagtttag tattgccgtc aatgctgctc
     9181 actttcagca gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg cagctgctcg
     9241 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
     9301 accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc cggctgcgga
     9361 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccaccacttt cggcagagca
     9421 atcagcaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg
     9481 ccggaggtga agtccatcac cgacaccgtg gtgtcatcaa tcttgatgtt gacgttgtcc
     9541 agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accgctatct
     9601 tcctcttctt caacctgttc agaaggaggc atttttttac tgtcgatgtt gacctgaatg
     9661 tctttcagcg ccaggtcatt aatacaaacg ctactgttcc acaggcactc aagaccgacc
     9721 cccagatgca gattacccgc ttttaccgcc acgcctagct gctcataacg aacgtcagac
     9781 aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg cacccagcga
     9841 tccgccgctt taaatacaag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
     9901 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
     9961 attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc gcgaccggta
    10021 cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
    10081 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
    10141 cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt atcaacttcg
    10201 aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat ttgtagccgc
    10261 gaatactgcg atcgcccccg acgaagaaac gcagatccgg cggtactttg tcgaaatcac
    10321 cggtttcaat ccagcccagc gtgccgcgtg tcacaaagcg atggcgatcg tacagtgtgc
    10381 ggatccagac gtgctgcgcc tggaaaacgg agaaatcgac atctgaaccc caggccgtgt
    10441 tggagtagtc gatagagtag cgttgcgagt ctccccaggt tggcatcagg ccaccacgag
    10501 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
    10561 cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc acgctgccag ccgctggaga
    10621 gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag
    10681 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagcggca
    10741 ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg gtggtcaaac
    10801 tgtgaccgta tgagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt
    10861 ccgtagagta accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg
    10921 tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
    10981 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcatact
    11041 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    11101 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    11161 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    11221 ctttacgcaa cgcaatactg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    11281 gtaccgtacc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    11341 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacacct ggcgtgactt
    11401 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    11461 gctggtaata acccagcgct ttcagacctt cacggatggc atcatcgacg cgtgcgcgaa
    11521 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct
    11581 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    11641 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    11701 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    11761 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    11821 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc
    11881 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    11941 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    12001 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    12061 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggcg
    12121 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    12181 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    12241 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    12301 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    12361 gttttcaggc ggcgatgctt gccgccgatg acgatcgtca catcaccacg gaaatcgcta
    12421 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccat
    12481 atggttattg tggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg
    12541 ggtacaaatg tagtttgttg ataaagtgct ctttgtctat gcctgatgcg gcttaaacga
    12601 cttaccaggc ctacaaaatc gcccaaattc aatatattgc aaggactgcg taggcctgat
    12661 cggcatagcg catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg
    12721 actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    12781 ggctttcaat gtgtatttca cacaaattaa tcaactttcc cttccgaggg tctggcctga
    12841 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt
    12901 gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg
    12961 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    13021 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc
    13081 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag
    13141 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    13201 tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt
    13261 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    13321 ggcctggcga acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    13381 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa
    13441 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca
    13501 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag
    13561 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    13621 atcatcggtt atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    13681 gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt
    13741 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    13801 tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat
    13861 ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    13921 gacggcggca ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag
    13981 tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa
    14041 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    14101 cgcatcgacc agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa
    14161 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct
    14221 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    14281 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    14341 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aatgtccttg atgtataacc
    14401 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    14461 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    14521 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    14581 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    14641 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    14701 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    14761 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    14821 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaatcggtc agcgagtgcc
    14881 gccgattggc attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    14941 aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    15001 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    15061 gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcgattc cgggttccgg
    15121 catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    15181 tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt
    15241 agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc tcacaccgga
    15301 agaggtgcag caagtgatgg acctgctgca aaagttgctc aaataatggc aataacataa
    15361 attctggaaa aataattttc ccctatcgtt atctatggga taggggaatt aagtctctga
    15421 gttcttacac cgaactaata ctggtaacgc tagttgctct tttttaaacg cctcaatggt
    15481 cgatttatat ttctcgcaaa tcttttcttt ggtaatatta ctatctgcgg ccagattcgc
    15541 acttactatc gcatccactt gttcggctaa ttcgacttgt tggcaacctg cattaaccag
    15601 aaaattataa ataagagagt aatcttcatc ctccaaaaca acagaggaac cattacgaaa
    15661 acgttcatcc cctccataga ttttaagcgc aaagcccaaa gattctatga ctatggcaaa
    15721 attatcatca attatttttt cgtaaatata ccatccatcg cattctttaa ttccaccgcc
    15781 tggtatttgg agttcaaatt taccccgttt taatttgctt actacatcct ccaataaggc
    15841 ataaccgaaa gatacgtcac caaacgcctg gcgaatataa cctttaatcc tttttggtat
    15901 gtcagactgt tttttctgca aaatatcaca ggcatacaat cttctgtttt gttctaacgg
    15961 aaactcgcgg tttttgaaat ccatgaatga gttatactca tttattgtta tgggaatttc
    16021 ttcgtattca aacccactgc ctcgcaatgt tattgtatat tcagcaccgt tatctttaag
    16081 gctgaaactg ataagaaaat tatccatggc attaacagtt tcattttttg atgaaagcac
    16141 ttgataacta tttttatcaa tctgtgttgc gcaacgaaag gactcccgca aagttaacag
    16201 actatcataa ttcgaaaccc aatctttttt cttaaatgca tgctgaatat tattaaaaaa
    16261 atcgtttgaa tgtgtttttg tatgcgatga ctttacaaat aactccaccg caccaataaa
    16321 attattgtgt ctgatcctgg gtaacatatg gctaccttat tttatttggt tattttatgt
    16381 cgccattaaa tatctcatta tagaagtaaa ctatcaaaaa acgctcatta ttaaaatgta
    16441 acttactagc aattgaattt ttgcacttta aatacaatct ctttttattt ataacttttt
    16501 aggttgggat aaatcaggat ggcagaatca ggaaatacac tgtttctgcc atctgaattt
    16561 agtaaatgga cactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg
    16621 gtttaccctg ccagtcgtga acgtgcgctc cggcagctgc agccacagca tgtccggcgg
    16681 cggtgtccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca
    16741 ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca
    16801 gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc ggcgggcgcg
    16861 catcgcggac ctgaatcagc ttgcgcacac cgcactcttc tttccaggcc ttgccttctg
    16921 ccgcgctgta cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccat
    16981 ggtcaatgag cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac
    17041 catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc caaccgggag
    17101 gatcttcttc agaaaggacc ggaatatccg gtgccagcgt acgtaaaccg tccatgataa
    17161 cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt
    17221 ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct
    17281 ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt
    17341 tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg
    17401 taaaattgtg gcattcttca ctgttctata agtaaggcgt ttattctccc ttttctttcg
    17461 tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac
    17521 gctgattgcc gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga
    17581 tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg
    17641 actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact
    17701 ggtcgataac ggcgatttga ttcaggggag tccgctggcc gattacatgt cggcgaaagg
    17761 attaaaagca ggtgatgttc atccggttta taaggcgctg aatacgctgg attatacggt
    17821 cggtacactc ggcaatcatg aatttaacta cggtctggat tacctgaaaa atgcgctggc
    17881 gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat
    17941 gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa aaaaacagac
    18001 gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa
    18061 tttatccgga aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga
    18121 aatgcgcgag aaaggtgccg atgtcattgt cgttctggcg cattccgggc tgtctgccga
    18181 tccgtataaa gtaatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa
    18241 cgccattatg tttggccatg ctcacgccgt tttcccgggt aaagattttg ctgatatcga
    18301 aggggctgat atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg
    18361 gggcgatcat cttggggtgg tcgacttaca actcagtaat gacagcggta aatggcaggt
    18421 gacgcaggcg aaagcggaag cacggccgat ttacgacatc gccaataaaa aatccctcgc
    18481 ggcggaagac agcaagctgg tagaaacact caaagccgat cacgatgcca cacgccagtt
    18541 cgtcagcaag ccaatcggta aatctgccga caatatgtat agctatctgg cgctggtgca
    18601 ggacgatccg accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat
    18661 tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgccgcac cgtttaaagt
    18721 tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt
    18781 ccgtaatgcc gccgatcttt atctctaccc caatacgctg attgtagtga aagccagcgg
    18841 taaagaggtg aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgctcctaa
    18901 cagcacgaaa ccacagtcac tcatcaactg ggatggtttc cgcacttata actttgatgt
    18961 tattgatggt gtgaattatc agattgatgt tacccaaccc gcccgctatg acggcgagtg
    19021 ccagatgatt aatgccaatg cagagcggat taagaacctg acctttaatg gcaagccgat
    19081 tgatccgaac gctatgttcc tggttgccac caataactat cgcgcttacg gcggcaaatt
    19141 tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct
    19201 ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcatccgg cggcagataa
    19261 caactggcgt ttagcaccga tagctgccga taagaaactg gatatccgtt tcgaaacttc
    19321 cccgtcagat aaagccgcag cgtttattaa agagaaaggg cagtatccga tgaataaagt
    19381 cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt aaaacacttc
    19441 tttttcggcc tataaatcaa ccgcatccgg catttattgg cggatgcgat gctgccgtat
    19501 cttatccacc ctacaagcca tgaaccgtag accggataag cgcagcgcat ccggcagtta
    19561 tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc
    19621 cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctat attccacatg
    19681 cggcggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa
    19741 cgactgcgca agcatctttt cactcacccc accgattttg cgccgcaggt cgctaaagcg
    19801 atgagtacct tcgcgtagcg ccaccagaat caacaccccc caacggctgg tgacgtgttt
    19861 caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca gttgttgtga
    19921 caggctaacc tgactcattt catacttacc tttttgcacg tacttactaa aagtaagttt
    19981 aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac
    20041 tggtgccact ggccaacttg gtcactatat tattgaatcc ttgatgaaaa cggttcctgc
    20101 cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg
    20161 cattaccgtg cgtcaggctg actacagcga tgaagccgca ctgacatctg cacttcaggg
    20221 agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc cgcagcatcg
    20281 taatgttatt aatgccgcaa aggcggctgg cgtgaaattt atcgcttata ccagcctgct
    20341 acatgcagat acctccccgc tcggcctcgc cgatgagcac atcgagacgg agaaaatgct
    20401 ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacagcg aaaactacct
    20461 cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggctg gcgatggcaa
    20521 aatcgcctca gcaacgcggg cagattatgc gacagctgcg gcacgcgtga ttagcgaagc
    20581 cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt
    20641 agcggcagag ctgaccaaac agagcggcaa acaggttacc tatcaaaatc tgagcgaagc
    20701 cgatttcgcc gcggcactga aaagcgtcgg actgcccgac ggactggcgg atatgctggc
    20761 ggattctgac gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa cgcttagcaa
    20821 attgattggc tgcccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa
    20881 taactagtta attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg
    20941 agcaatcatg tctgcttcct gaacgtttct tctgacagac caatggatgc cagtaatgat
    21001 tagcggcgtg ctgtacgccc tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc
    21061 gttgatcgtg ccggaatacc cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg
    21121 gttaattgcg ctacccattg cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg
    21181 ggactggctt accgccttga tgctcactat gatgggcaac ctcatttatt acttctgcct
    21241 tgccagtgcc attcaacgta ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc
    21301 ggtggtgatt cctgtctttg ccaatctgct ttatagccag cgcgacggca aactcgcgtg
    21361 gggaaaactc gccccggcac tgatttgtat tggcatcggc ctggcgagtg tgaatattgc
    21421 tgagttaaac cacggactcc ccgattttga ctgggcacgt tatacctctg gcatcgtgct
    21481 agcgttagtt tccgtggtct gctgggcatg gtatgccctg cgcaacgccc gctggctgcg
    21541 ggaaaatccc gacaaacatc cgatgatgtg ggcgacggcg caggcgctgg tcacgctgcc
    21601 ggtttctctc atcggctatc tcgtcgcctg ttactggctg aatatacaaa cgccggactt
    21661 ctccttacct tttggccccc gtccgctggt gtttattagt ctgatggttg cgatagccgt
    21721 gctttgctca tgggttggcg cactctgctg gaacgtcgcc agccagcgat taccgacagt
    21781 gattctcggg ccgctgattg ttttcgaaac gctggcaggt ttgctgtaca cctttttact
    21841 ccgccagcaa atgccgccgc taatgacgct gagcggtatc gcgctgttag tgattggcgt
    21901 ggtcatcgcg gtcagagcaa aaccagaaaa acctttaact gaatctgtct cagaaagttg
    21961 acatgctggc agtgagttaa ataagcctct gctacgtaag ggttatagct tttgccttaa
    22021 aggtgtattt aaaatacatc ttatcttatt aagaatgagg tatcagctat ggcttatcgc
    22081 gaccaacctt taggtgaact ggcgctctct atccctcgcg cttcggctct gtttcgtaaa
    22141 tatgatatgg attactgctg tggcggtaag cagacgctgg cgcgcgcggc ggcacgtaaa
    22201 gaactggatg ttgaggtcat tgaagctgaa ctggcaaagc ttgctgaaca accgattgag
    22261 aaagactggc gtagcgcccc gctggcagaa atcattgacc atatcatcgt gcgctaccac
    22321 gatcgtcacc gcgagcagct gccggagctg atcctgcaag cgactaaagt cgagcgcgtt
    22381 cacgccgaca aaccgagcgt gccaaaaggg ctgacaaaat acctgaccat gctgcatgaa
    22441 gagctttcca gccacatgat gaaagaagag cagatcctct tcccgatgat caaacaaggc
    22501 atgggcagcc aggcaatggg gccaatcagc gtaatggaaa gcgagcacga tgaagcgggc
    22561 gaactgctgg aagtgatcaa acacactacc aataacgtca caccgccgcc agaagcctgc
    22621 accacctgga aagcgatgta taacggcatt aatgaactga ttgatgacct gatggatcac
    22681 atcagtctgg aaaacaatgt actgttccca cgcgccctag cgggtgagtg atctaaaagc
    22741 tggatggcat tgcgccatcc agcatgataa tgcggttatt tccgcagttc agcagcccgc
    22801 ttcttaccaa taaacagcca gcccaacccc agcgcgataa accacagcgg ggtaaccagc
    22861 agcgcctggc gagtgtcatc ttccagtgtc agcaacacga ccacgaacac aaagaacgcc
    22921 atacataccc agcacatcag cttgccgagc ggcatcttgt agatcgactt ttcatgcaga
    22981 tgaggacgct gtttgcggta caccaggtaa gagcaaagga taatcgtcca gacgaacata
    23041 aacagaatcg cggaaacggt tgtaatcatc gtgaacgcgc caatcacgct aggattcaca
    23101 tacaacatca ccacgccgcc gagcagacag atacacgaga aggtcaaccc tttcgcgggt
    23161 actgcgcgct tagaaagttt agcgaacgct ttcggcgcaa caccttcctg cgccagacca
    23221 aacagcatac ggctggtaga gaagacaccg ctgttagcgg aagacgccgc agaggtcagc
    23281 accacaaagt tgatcacgct ggcagccgca ggcaaaccta ccaacacaaa cagctcaacg
    23341 aacgggcttt tctccgggac taccgaactc cacggtgtca cggacataat cacaatcagc
    23401 gcgaagacgt agaacataat gatacggatc ggaatggagt taatcgcgcg tggcagtgat
    23461 ttctccggat ctttggtttc cgcagctgtt gtacctacca gctcaatccc cacgaaagcg
    23521 aaaaccgcta tctggaatcc ggcaaagaag ccacttaaac ctttcgggaa ccagccgccg
    23581 tcattccaca aatgcgcgaa tgacgcttcc acaccggtcg gtgactgaaa gtgcatcgcc
    23641 accatgacca ggccgacgac aatcagcgac acgatggcga cgattttgat catcgcaaac
    23701 cagaactcca tctcaccgaa cattttcacg gtggcaaggt tgagcgtcag cagcagcact
    23761 atcaccgcca gcgaggcgac ccagtcggag agatcgggga accagaactg agcataagcc
    23821 gtgatcgcca ccacgtctgc cataccggtt acaacccagc agaaccagta agtccagccg
    23881 gtgaaatatc ccgcccacgg ccccagtaaa tcggaagcga agtcactaaa agatttgtat
    23941 tccagattcg aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag cataaaacca
    24001 atgatcatat aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt gccggacccc
    24061 ataaacaacc cagtaccaat ggcacctcca atggcaataa gctgaacatg tcggtttgtg
    24121 agattgcgcc gtagcgactg ttcagccgga gcctgatcat cggcaacgac ttttacctga
    24181 tctaccatgt tttttcttcc tgtacctgtc tgtgttgttc aggctctacg gcctttagtc
    24241 agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggtaaca atacgcggga
    24301 tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt catatcacgg
    24361 tgataacgcg aaacagctca caaaaataca cgcttacggg gtctttgcaa cacaaaatag
    24421 cgccgtttgg caaactgaag ggtttattgc tgaatgcctg ctcccctctg gttcgagggg
    24481 aacagaatgc gctgcttaga ggatttccag cagttcgact tcaaacacca gggtgctgaa
    24541 cggagggatg gatgcacctg cgccgcgctc gccatatgcc agttcctgcg ggatagtcag
    24601 ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag ggatcacgcc
    24661 attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc
    24721 gatcagttta ccggtgtaat gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc
    24781 accctggttg atcacgcgga attgcaggcc agattcggtg ctattcacac cttctttttt
    24841 ggcgttttct tccaggtatt tcacgccttc agcagccatc gcctggaaac gctgacgacg
    24901 aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat caaccggcac
    24961 agccggatgt ttgccttcca gcgcatcggc aatacctgca accagtgctt ctggcagcag
    25021 cccttccagg ccagattcac tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc
    25081 ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct tagggtataa
    25141 aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc aggaatgatg
    25201 acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt
    25261 ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa caagacgtgg
    25321 agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc
    25381 acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca
    25441 ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg
    25501 cgtcggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag
    25561 aagaacagcc gcaaactacc caaacgcaac cattccagcc agatagcgga attgataatc
    25621 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg
    25681 gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga
    25741 gtaatttgca aaatgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
    25801 gcttaaccat tgatacgggg aataatcaac aggtcctgtt tacccgccag ccggatggca
    25861 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
    25921 gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatat gataaactag
    25981 gccaagcgta tgctggttga tcaaataata tcccccaagc gcagcaatat taatattcac
    26041 ctgcaatggg tcgatacctg gcttaaaaat attgtatttc ttcccttcat ccaataagga
    26101 ttccattaac tgcaagtgag catggttaat ttctaagaga cgctgtgatt ttgcataatg
    26161 cacaccttta ctttgatttt cactatgcac aatttttaaa aaccaaggat tagcaatata
    26221 ataatcccat gtaaaatcaa taattttctc aatggctttt tgtggcggaa gatctgagaa
    26281 atcaagggcc ttttcttttt gtcgaatatc gttccaggta tattccagca cttcgataaa
    26341 taaattttct ttattaccaa agtggtgata aaccgtctgt ttactacacc ctgctgcctt
    26401 tacaatatta tctactctgg ccccttcata accatattcg gcaaattcat taaccgcaca
    26461 tagcagcaat ttttccttca ggctctgtac gttgctggta tcaatataca taacgcttcc
    26521 ccacgacctc ttcgtggtga ctatcataac ctgatttgat ttcagcttca gaaaaaaggg
    26581 caaaaacttg tactttcatc aggttatcaa atgtcctgtg atcgccatcg catatcaacc
    26641 aactagatgg tttatctttt tatcctccag tcgcaagcta atcatcacca aggaaacaaa
    26701 attcaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtga ttaccggagc
    26761 cactcgcggt attggaaagg gctgtgcgca ggagcttgct cgtggcgggt ttaacctgct
    26821 gattaacgat cgtcccgatg ccgacagcgt agaaaaactg catatcacac aacaagaatg
    26881 tttggcggaa ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc tatctctgca
    26941 tgaggagatg ctcgacgcgg cgcaaaacca gtgggggcgg ctggactgtc tgctcaataa
    27001 cgcggggatt tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag acagcttcga
    27061 tcaaaacatt gccattaaca cccgcgcgcc gttcttcctt gctcaggcat tcagtaagcg
    27121 cttactggca cagccgaaac cggaagcgga gctgccgcac cgctcgatta ttttcgtcag
    27181 ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg ccaaaaccgc
    27241 cgtttctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa ttggcgttta
    27301 tgaagtgcgc cctggtctca tcaaaactga tatgactatt ccggcaacgg cttattacga
    27361 cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg ctgatattgc
    27421 ttccaccgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc aggcggtcgc
    27481 aatcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag atctgttaca
    27541 acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc cgctacccgc
    27601 cattattgat gcctgcgccg cacgcggtat tggcgcaatt gccccctggc gcagggagtt
    27661 gcagggtgaa gatctccagc aaatcacccg ccagcttgcc gccagtaaca tgagcgtttc
    27721 cggtttgtgc cgtagcactt actacaccgc gccgacactg gcggaacgca aactggcgat
    27781 tgatgataac cgccgggcgc tggacgacgc
//
LOCUS       DAJVPT010000051        27535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVPT010000051 DAJVPT010000000
VERSION     DAJVPT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQM47_002111"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQM47_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1480264.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQM47_002112"
     CDS             complement(1614..1937)
                     /locus_tag="OQM47_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1480265.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM47_002113"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM47_002113"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1480266.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM47_002114"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM47_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1480267.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM47_002115"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM47_002115"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1480268.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM47_002116"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM47_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1480269.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQM47_002117"
     CDS             7537..8574
                     /locus_tag="OQM47_002117"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1480270.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM47_002118"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM47_002118"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1480271.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQM47_002119"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQM47_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1480272.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM47_002120"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM47_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1480273.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQM47_002121"
     CDS             10745..11626
                     /locus_tag="OQM47_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1480274.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM47_002122"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM47_002122"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1480275.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQM47_002123"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQM47_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1480276.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM47_002124"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM47_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQM47_002125"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQM47_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1480277.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM47_002126"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM47_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1480278.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQM47_002127"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQM47_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1480279.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQM47_002128"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQM47_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1480280.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQM47_002129"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQM47_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1480281.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQM47_002130"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQM47_002130"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1480282.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQM47_002131"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQM47_002131"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1480283.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQM47_002132"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQM47_002132"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1480284.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQM47_002133"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQM47_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1480285.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQM47_002134"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQM47_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1480286.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM47_002135"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM47_002135"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1480287.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQM47_002136"
     CDS             24893..25273
                     /locus_tag="OQM47_002136"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1480288.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQM47_002137"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQM47_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1480289.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM47_002138"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM47_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1480290.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="OQM47_002139"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="OQM47_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6790 a         6852 c         7222 g         6671 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DAJVPT010000052        26932 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVPT010000052 DAJVPT010000000
VERSION     DAJVPT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..627
                     /locus_tag="OQM47_002140"
     CDS             <1..627
                     /locus_tag="OQM47_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1480291.1"
                     /translation="SISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILA
                     EHHLLDYKGVEKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTT
                     AMYIRQLLAQRHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGF
                     PLINAVADMFKGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            618..1091
                     /locus_tag="OQM47_002141"
     CDS             618..1091
                     /locus_tag="OQM47_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1480292.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1198..1737
                     /gene="dnaT"
                     /locus_tag="OQM47_002142"
     CDS             1198..1737
                     /gene="dnaT"
                     /locus_tag="OQM47_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1480293.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1740..2477
                     /gene="dnaC"
                     /locus_tag="OQM47_002143"
     CDS             1740..2477
                     /gene="dnaC"
                     /locus_tag="OQM47_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1480294.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2526..3020
                     /locus_tag="OQM47_002144"
     CDS             2526..3020
                     /locus_tag="OQM47_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1480295.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3260..5551
                     /gene="opgB"
                     /locus_tag="OQM47_002145"
     CDS             3260..5551
                     /gene="opgB"
                     /locus_tag="OQM47_002145"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1480296.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5689..6711)
                     /gene="lgoD"
                     /locus_tag="OQM47_002146"
                     /pseudo
     CDS             complement(5689..6711)
                     /gene="lgoD"
                     /locus_tag="OQM47_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            6850..7764
                     /locus_tag="OQM47_002147"
     CDS             6850..7764
                     /locus_tag="OQM47_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1480297.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            7979..9340
                     /locus_tag="OQM47_002148"
     CDS             7979..9340
                     /locus_tag="OQM47_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480298.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9389..11053)
                     /gene="tsr"
                     /locus_tag="OQM47_002149"
     CDS             complement(9389..11053)
                     /gene="tsr"
                     /locus_tag="OQM47_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1480299.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11172..11618)
                     /gene="hpaR"
                     /locus_tag="OQM47_002150"
     CDS             complement(11172..11618)
                     /gene="hpaR"
                     /locus_tag="OQM47_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1480300.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            11890..13179
                     /locus_tag="OQM47_002151"
     CDS             11890..13179
                     /locus_tag="OQM47_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1480301.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13176..14642
                     /gene="hpaE"
                     /locus_tag="OQM47_002152"
     CDS             13176..14642
                     /gene="hpaE"
                     /locus_tag="OQM47_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1480302.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14644..15495
                     /gene="hpaD"
                     /locus_tag="OQM47_002153"
     CDS             14644..15495
                     /gene="hpaD"
                     /locus_tag="OQM47_002153"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1480303.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15505..15885
                     /locus_tag="OQM47_002154"
     CDS             15505..15885
                     /locus_tag="OQM47_002154"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1480304.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            15953..16756
                     /gene="hpaH"
                     /locus_tag="OQM47_002155"
     CDS             15953..16756
                     /gene="hpaH"
                     /locus_tag="OQM47_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1480305.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            16767..17570
                     /gene="hpaI"
                     /locus_tag="OQM47_002156"
     CDS             16767..17570
                     /gene="hpaI"
                     /locus_tag="OQM47_002156"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1480306.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17592..18947
                     /gene="hpaX"
                     /locus_tag="OQM47_002157"
     CDS             17592..18947
                     /gene="hpaX"
                     /locus_tag="OQM47_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1480307.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            18957..19847
                     /gene="hpaA"
                     /locus_tag="OQM47_002158"
     CDS             18957..19847
                     /gene="hpaA"
                     /locus_tag="OQM47_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1480308.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            19874..20811
                     /locus_tag="OQM47_002159"
                     /pseudo
     CDS             19874..20811
                     /locus_tag="OQM47_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21038..22596
                     /gene="hpaB"
                     /locus_tag="OQM47_002160"
                     /pseudo
     CDS             21038..22596
                     /gene="hpaB"
                     /locus_tag="OQM47_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22614..23126
                     /locus_tag="OQM47_002161"
     CDS             22614..23126
                     /locus_tag="OQM47_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1480309.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23519..25669
                     /gene="btsT"
                     /locus_tag="OQM47_002162"
     CDS             23519..25669
                     /gene="btsT"
                     /locus_tag="OQM47_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1480310.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25719..25922
                     /locus_tag="OQM47_002163"
     CDS             25719..25922
                     /locus_tag="OQM47_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1480311.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25933..26889
                     /gene="yjiA"
                     /locus_tag="OQM47_002164"
     CDS             25933..26889
                     /gene="yjiA"
                     /locus_tag="OQM47_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1480312.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         6485 a         7138 c         6884 g         6425 t
ORIGIN      
        1 tctatctctt cgaacgccat agtgctgaca actattaaag atgggcaatg cctgacatcg
       61 acacgtaaaa atcacgatcg cggcattaat atgcatgtgg tgactgaagt ccagcacatt
      121 gtgattcttg cggagcatca tctgctggat tacaaaggcg tagagaaacg atttagccaa
      181 attcaaccat tacgttaccc aagatggctg gtagccttaa tggttggcct ttcttgcgcc
      241 tgtttctgta aactcaataa cggtggctgg gatggtgccg tcatcacctt ctttgccagt
      301 acgaccgcga tgtatatccg ccagctgctg gcacaacgtc atcttcatcc acagatcaac
      361 ttttgcctta ccgctttcgc cgccaccacc atttccggat tgcttttgca actccccact
      421 ttcagcaata cccccaccat tgcgatggcc gccagcgttc tgctgctagt gccgggcttt
      481 ccgttgatta atgccgtcgc cgatatgttt aaaggccaca tcaataccgg actggcacgc
      541 tgggcgatcg ccagtctgct gacactggct acctgcgtcg gcgtagtgat ggcactgacg
      601 atttgggggc tacgcggatg ggtgtgatcg aatttctgtt agcgttggcg caggatatga
      661 tcctcgccgc cattcctgcg gtcggctttg cgatggtgtt caacgttccc gtgcgggcgt
      721 tacgctggtg tgcgctgctt ggctcgatag gtcatggttc ccgaatgatc ttgatgacca
      781 gcgggttgaa tattgagtgg tcaaccttta tggcttctat gctggtcggt accattggta
      841 ttcaatggtc gcgctggtat ctggcgcatc cgaaagtgtt taccgtggcg gccgttatcc
      901 ctatgttccc gggcatatcg gcttataccg caatgatttc gtcggtaaaa atcagccagt
      961 taggttacag cgaaccgttg atgattaccc tgttaaccaa ctttcttaca gcttcatcga
     1021 ttgttggtgc gttatccatc ggtctttcca ttcctggatt atggttgtac cgcaagcgcc
     1081 ctcgcgtata aaattgcctc gcttaagcac acggatgaga gacagcctcc tctcctccgt
     1141 gtgttactat aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg
     1201 tcttccagag ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa
     1261 accgttctgg caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg
     1321 ttttatgccg tcacgcctgc acgcctggct gaactgctgg cgctggaaga aaagctagcg
     1381 cgtccgggaa gcgatgtcgc tctggacgat caactctatc aggaaccgca agccgctccc
     1441 gttgctgtac ccatggggaa attcgccatg tatccggact ggcaacccga tgccgatttt
     1501 atccgcctgg cggcgctatg gggcgtggcg ctaagagagc cggtgaccac cgaagaactg
     1561 gcctcattca ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa
     1621 caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga
     1681 gatgtgaata cggtcagtga acctgacagc caaattccac caggattcag agggtaacga
     1741 tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac
     1801 ctgcattcaa aacgggtgaa gagctactgg cctggcagaa agaacaaggg gcgatccgct
     1861 ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg
     1921 gtattcgtcc actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc
     1981 agatgaatgc gttaagcaaa gcgcgccagt atgtcgaaga gtttgacggc aacatcgcca
     2041 gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca
     2101 acgagctgct gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg
     2161 cgatgaaaga taccttcagg aatagcggta ccagcgaaga acaactgctt aacgatctga
     2221 gcaacgttga tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa
     2281 aagtgatcat caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc
     2341 tgaccaacag taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta
     2401 tgcgcctggg taacagttta tgggtgatct tcaactggga tagctaccgt agccgggtaa
     2461 cgggtaaaga gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag
     2521 taatgatgat gaaaactatt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa
     2581 tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac
     2641 ttggcaacca aaacagcacg acacaggaag gcggttggtc gctcgcgtca ttaactaact
     2701 tgcttagcag cggaaaccaa gccttaagcg cagataacat gaacaatgcc gcaggcattc
     2761 tgcaatactg cgcgaagcaa aagctggctt cggtaaccga tgccgaaaac atcaagaacc
     2821 aggtgctgga aaagctgggc ctgaacagtg aagagcaaaa agaagacacc aactatctgg
     2881 acggtattca gggtttgctg aaaacaaaag atggtcagca actcaatctg gataacatcg
     2941 gaacgactcc gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg
     3001 ggctgaactt catttcctga accgcgaaat gttacccggc tgacgcggct ctctcctgcc
     3061 cttttccctg ctcctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa
     3121 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     3181 atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg
     3241 gtttagtgag gatcgcccat tgtcagaact actctctttc gccctttttc tcgcctctgt
     3301 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cgttaacggt
     3361 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga
     3421 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     3481 caaatacatc ctgccgggta tcggcattgt gctggggctg acggcggtgt tcggtgcgct
     3541 gggctggatc ctgcgccgtc gtcgtcatca tccgcaccat tttggttaca gcctgctggc
     3601 gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     3661 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     3721 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     3781 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     3841 tgaaggcctg gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg
     3901 catggtggct tctcagtgcg gcataccgct gttcgccccc tttgaaggca acgcctccgc
     3961 ctctgtctcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     4021 ttatcagaac tatttcgtgc agggcgcaaa tctgcgtttt gccggtaaag atgtgttcct
     4081 gaaatcgcac ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtagccga
     4141 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     4201 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     4261 tacccatcac ccggatggtt ttatctcgcg cacctgtaac cgcaaaaaat atgattttga
     4321 cggtaagccg aatcagtcat tcagcgcggt aagttgcagc caggagaata tcgcgacgtt
     4381 tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg tctcttctga
     4441 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     4501 gttttttgtc attcgtggcg acaagccgca gcaaaagacg ctggcagtga agcgtaacac
     4561 gatggataac ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg
     4621 tcgtagcagt ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac
     4681 attggcgtgg aagccagata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     4741 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     4801 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc
     4861 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgacagttg
     4921 ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact ggtgtgtctc
     4981 gcaggggcag cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa catggaagag
     5041 caagacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca atgtcgatac
     5101 gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct ttaacgtcgc
     5161 cggtgcgcca gaagaggtga aacagtttag cgggatttcc cgtccggagt cgtggggccg
     5221 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa
     5281 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     5341 ggtacgcgta ggcaatgaag aacaaactct tgtgctgggc aatgaagtga ccaccaccac
     5401 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     5461 ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt
     5521 ggaaattaaa gtggtagaac gtgaagggta attcctgact gatgtgtagg tcgcataggg
     5581 tgcgacctac acgccttctc cggcttacaa cacattatta agaaacaaaa aaaacagcgc
     5641 ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat
     5701 tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt
     5761 ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg attttccctt ccgccatcag
     5821 tcgaccgact ttagcgaaat cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc
     5881 ttttttatgg aattccggat cggagaactg caactcacct ttaaacagac cgacaaatac
     5941 caccgtgccg ccgtgacgaa tcagattcac ggtattattc atcgcatgtt gattacctgt
     6001 cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa
     6061 atcttcggct gacgggtcca gcacgggcaa ctccagacgc gttgccacat gttcacggcg
     6121 cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc
     6181 gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc
     6241 gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg cctgcgggtc
     6301 aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc
     6361 atcctgatgc acgccaatga ctgaaatttt ttcacagcag ttggtacgcc cgcttttaca
     6421 cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac catttttaag
     6481 atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacccg
     6541 tggataacta aaaaatggtt gatttccacc ctaggcatga atatcggtac cgcaaatccc
     6601 gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt
     6661 ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct
     6721 atccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat
     6781 ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag
     6841 gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat
     6901 gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg
     6961 tacaacatca gccgcactac agtgcgtcat attctcagcc aattacgcga atgcggcgtc
     7021 ttaacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc
     7081 gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg
     7141 atgataaacc agcgtcagtt acgccctagg gaaacttttt ctgaactgca actggctcgg
     7201 gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat
     7261 cttattcaaa gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc
     7321 gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac
     7381 ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctagaacg tcatcggcta
     7441 ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac
     7501 tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     7561 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     7621 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     7681 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     7741 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     7801 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     7861 actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc
     7921 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     7981 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8041 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8101 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8161 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8221 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     8281 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     8341 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     8401 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     8461 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgctgt
     8521 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     8581 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     8641 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     8701 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     8761 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     8821 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     8881 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     8941 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9001 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9061 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9121 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9181 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9241 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
     9301 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
     9361 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
     9421 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
     9481 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
     9541 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
     9601 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
     9661 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
     9721 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
     9781 ccgagtcttc aatcaggctt ttaatttcac gaaccgcctg ggcgctacgc tgggccagat
     9841 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
     9901 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
     9961 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10021 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10081 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10141 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10201 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10261 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    10321 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    10381 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    10441 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    10501 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    10561 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    10621 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    10681 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    10741 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    10801 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    10861 tacgcgtctg caacaacgcg acccagctgc cattcagcgt gggttgctgc tggcgaatgg
    10921 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    10981 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11041 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11101 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11161 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11221 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    11281 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    11341 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    11401 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    11461 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    11521 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    11581 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    11641 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    11701 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    11761 tctttaaatg taaatagttt gttaattgga tcacatttat atcacttact tttgccaaac
    11821 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    11881 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    11941 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12001 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12061 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12121 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggataacga cgtcagcctg
    12181 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12241 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    12301 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    12361 agcgccctga gcgaatttgc cacgctgaat ccaagcgatg ctattctgct cggcacgcca
    12421 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    12481 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    12541 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    12601 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    12661 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    12721 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    12781 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    12841 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    12901 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    12961 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13021 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13081 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13141 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13201 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13261 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    13321 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    13381 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    13441 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    13501 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    13561 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggcgtcg ccgtggaacg
    13621 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    13681 tgctgaagat gtcggaactc tccccgctga ccgccgaccg cctgggtgag ctggcgctgg
    13741 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    13801 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    13861 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    13921 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    13981 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaga
    14041 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14101 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14161 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14221 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    14281 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    14341 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    14401 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    14461 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    14521 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    14581 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    14641 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    14701 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    14761 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    14821 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    14881 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    14941 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15001 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15061 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15121 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15181 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15241 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    15301 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    15361 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    15421 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    15481 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    15541 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    15601 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    15661 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    15721 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    15781 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    15841 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    15901 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    15961 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16021 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16081 gcgcgaatgg gttcgcctga aaatcgccga aagtcgcacg ctgaaaggcc acaaaatcgg
    16141 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16201 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16261 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    16321 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    16381 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    16441 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    16501 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    16561 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    16621 accgtatgac gtacaactgg aaaccgggca aatcattctc ggtggttcgt tcaccctccc
    16681 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    16741 ctgccgcttt gtttaaggag agaacgatgg aaaacagttt taaagcggcg ctgaaagcag
    16801 gccgtccgca gattggatta tggctggggc tgagcagcag ctacagcgcg gagttactgg
    16861 ccggaccagg attcgactgg ttattgatcg acggtgagca cgcgccgaat aacgtgcaaa
    16921 ccgtgctcac ccaactacag gcgattgcgc cctaccccag ccagccagtg gtacgcccat
    16981 cgtggaacga tccggtgcaa atcaaacaac tgctggacgt aggcacacaa accttactgg
    17041 tgccgatggt acaaaacgcc gacgaagccc gtaaagcggt acgcgccacc cgttatcccc
    17101 ccgccggtat tcgcggtgtg ggcagtgcgc tggctcgcgc ctcgcgctgg aatcgcattc
    17161 ctgattacct gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg
    17221 aggcaatgaa gaacttaccg cagattctgg acgtggaagg ggtcgacggc gtgtttatcg
    17281 gcccggcgga tctgagcgcc gatatgggtt atgccggtaa tccgcagcac ccggaagtac
    17341 aggccgccat tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc
    17401 tgatcgccaa tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgccg
    17461 tcggcgttga caccaccctg ctcgcccgcg ccgccgaagc gctggcagca cggtttggta
    17521 atagttcttc tatttcgatg gcgaagcaaa acaacaactc tgtctactaa acgtacgatg
    17581 tggagcgcat catgacgacc tctaccctgc aatataaaga taataaaact gttgaaattg
    17641 aacatcgtgt cattaataaa ttatttcgtc gcctaatcat ttttcttttt attctgtttg
    17701 ttttttcgtt tcttgatcgc atcaatatag gatttgccgg actgacaatg ggcaaggatc
    17761 ttggcctgac gtccacaatg tttggcctcg ccgcaacact cttttatgtc acgtacgtca
    17821 tgtgcgggat ccccagtaat atgatgttag cgaaaattgg cgctcgtcga tggatcgctg
    17881 ggatcatggt ggtctgggga attgcttcca cctgcacaat gtttgcaaca agccccgaaa
    17941 cactctatct actgcgtatg ctggttggta ttgctgaggc gggtttttta cctggtattt
    18001 tggtttatct gacctggtgg ttcccggcat atcatcgggc cagagccaat gccttattta
    18061 tgatagcgat gccagtcact atgatgctgg gattgatcct ttcaggctac attctggcct
    18121 tggatggact gtggaatcta aaaggctggc aatggctctt tttgctggaa ggtctgccat
    18181 cggtcattct tggtgtcgtc acctggtttt atctcaacga cacaccagac caggcgacct
    18241 ggcttgatga aaacgaaaaa caggctttaa aaacgatgat tctccgtgaa caagagagtg
    18301 catttgccca tgcatctgtt ccacgctcaa ccttacgtga agtcctgacc ccagccatca
    18361 tgctatatac acttgcttat ttttgcctga ctaatactct gagtgcgatt aatatctgga
    18421 cgccgcaaat cctacagagt ttcaatactg gcagtagcaa catcatgatt ggtttgctgg
    18481 cggccatccc ccaattctgc acaatttttg gcatgatctg gtggagtcgt cgttccgacc
    18541 gtcttaaaga acgcaaaaag catacgattc ttccctacct gtttgccgct gccggatggt
    18601 tactcgcttc tgcgacagat cacagcctca ttcaactgag cagcatcatt atggcctcaa
    18661 tgggatcatt taccgcgatg actatctttt ggacaacacc ggatcaagtt ataagcttgc
    18721 aatcgcgtgc cgttgcgatg gctgtcatca atgctgtcgg caatgtaggt tcagcagtca
    18781 gtccattact tatcggtatt ttgaaagatg caaccggcag ttttagctca ggtttgtggt
    18841 ttgtagcagg attgctggta ctgggcgcgt tagtcatgac ccgaatccct atggggagtc
    18901 gggaacactc tctttcatct gaacttatcg caccaaaacc gcaataagga gtcatgatgt
    18961 ccagtgaagc tatctttgaa catggttatg aaaatattga tttggtccgg gaatatgatg
    19021 cccgctacgc tggtgaagat gtgcattatg aaacatttgc ccggttagcg aatttctttg
    19081 gacgcgatat gcgccctcac tggcacgata gatattttca actccattat ctggtcactg
    19141 gacgcattac tttacaactg gatgagcact tctattctgt gcgcgcaccg ctttttgtac
    19201 taacaccacc atcggtgcca catactttct ttactgatcc tgatagcgat gggcatgttc
    19261 tgaccgtacg tcaggagtta atttggcccc tggtagaaaa gctctggcct ggtagtggag
    19321 atgccgcaat gctacaaggt atctgtctgt cactggaggg aatggaacat acactggcgg
    19381 cattaaatca ttattggccg ataatatccc tggagtttca acaaaataag caaggacggg
    19441 aaattctatt gcaatcactg gcgcaaagta tttttacaat cctactgcgt gaagcaccac
    19501 caaatgatat ttcaacatgc agcgtgcgcg gcgagatgtg tttattccag aagtttaatc
    19561 ggctgattga cgaaaattat cgccaacatt taatggtccc tgaatatgca acaatgctcg
    19621 gtatgagtga atcacggctt accgagctgt gccgacgttt tgccaatcaa tcgccgaaga
    19681 gattgatttt cgaacgagtg agccgggaag ccagacggct tctgctctat agcgagcaaa
    19741 gcatcaacca gatagccctt gatcttggct acaaagatcc cgcctatttc gcccgttttt
    19801 tcaaccgaat gatgggttgc tcaccaacac aattccgtgg tcgctgacca tttatcaacc
    19861 tgagagactc gacatgaaaa tatctttacg ctcactattt gtggcgtgcc tgattgcgcc
    19921 agtggcattc acctacgcct ccgaaccgca ataaatgaaa attgtactac ttggaacgaa
    19981 aggtggccct tctttattaa aagctgaccg tttaccgatg gcaacggcac ttattattgg
    20041 caataaaacc tggttaattg atgctggtta cggggccagc ttacaactgg taaaaaataa
    20101 tattccactg cgccagattg ataatatttt actcacccat ttacacagtg atcatattct
    20161 ggactatcca tcagtcttaa tgaatgcatg ggctagcggt ctgcaggaca aaactattca
    20221 tgtattcggt ccgccaggca cccgagccat gaccgctgcc agttggcagg tcttcgatcg
    20281 ggacatcaca ctgcgtatgg aagaagaagg aaaacccgat cctcgcaaac tcgttacagc
    20341 tcaagatgtt ggtgaaggcg tagtttataa agatgatacc gtgaccgtga ctgcattaaa
    20401 ggtccctcat tctcctttcc ctgacggtga ggcttttgct tatcgcttcg acacacaagg
    20461 caagcgaatt gtcttctcag gagatacctc gtactttccc ccactggcaa ctttctcaga
    20521 aggcgctgat atacttgtgc atgaggccgt acatgagccc tccgtcgcta agctggcaga
    20581 cagtattggc aatggtaaaa cactggcaaa agccattgct tctcaccata cgactattga
    20641 ggatgtaggc aaaattgcca atgaagcaaa agtgaaaaaa ctcatcctta gccacctggt
    20701 accaatcacc gttccggatg aaacatggaa gcaagaagca cagaaaacta ttcaggtcct
    20761 atcgtcgttg gtcatgaaaa tatgacgatt accattccat tcgccccata aaaaattccg
    20821 tccccgttag cccttgttag cggggaatat tatgatttgc cttactgtaa gttgatcacg
    20881 atcgcagttt actatttaac tccaccttgc tgtcgaaaag taccaacgtt cgcctgaaac
    20941 gtctcttccc gacaaccgcg ttcaacgctt caattaaata acaaaaacaa cacacaaaat
    21001 taacaatcgt attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc
    21061 cagtacccaa cgtcctttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga
    21121 gatctatatc tatggcgagc gagtgaaaga cgtcaccact catccggcat ttcgtaatgc
    21181 ggcagcgtct gttgcccaac tgtacgacgc gctgcacaaa ccggagatgc aggactctct
    21241 gtgttggaac accgacaccg gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa
    21301 aagtgccgac gacctgcgcc agcaacgcga cgccatcgct gaatggtcac gcctgagcta
    21361 tggctggatg ggccgtaccc cagactacaa agccgctttc ggttgcgcac tgggcgcgaa
    21421 tccgggcttt tacggtcagt tcgagcagaa cgcccgtaac tggtataccc gtattcagga
    21481 aactggcctc tactttaacc acgcgattgt taacccaccg atcgtcattt gccgaccgat
    21541 aaagtgaaag acgtttacat caagctggaa aaagagactg acgccgggat tatcgtcagc
    21601 ggtgcgaaag tggttgccac caactcggcg ctgactcact acaacatgat tggcttcggc
    21661 tcggcacaag tgatgggcga aaacccggac ttcgcactga tgttcgttgc gccaatggat
    21721 gccgatggcg tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc
    21781 tcgccatacg actacccgct ctccagccgc ttcgatgaga acgatgcgat tctggtgatg
    21841 gataacgtac tgatcccatg ggaaaacgtg ctgatctacc gcgattttga tcgctgccgt
    21901 cgctggacga tggaaggcgg tttcgcccgt atgtatccgc tgcaagcctg tgtgcgcctg
    21961 gcagtgaaac tcgacttcat tacggcactg ctgaaaaaat cactcgaatg taccggcacc
    22021 ctggagttcc gtggtgtgca ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc
    22081 tgggcattga gtgactcgat gtgttcagaa gcgacgccgt gggtcaacgg ggcttattta
    22141 ccggatcatg ccgcactgca aacctatcgc gtactggcac caatggccta cgcgaagatc
    22201 aaaaacatta tagaacgcaa cgttaccagt ggcctgatct acctcccttc cagtgcccgt
    22261 gacctgaaca atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt
    22321 atggatcacg tccagcgcat caagatcctc aaactgatgt gggacgccat tggcagcgaa
    22381 tttggtggtc gtcacgagct gtatgaaatc aactactccg gtagccagga tgagattcgc
    22441 ctgcaatgtc tgcgccaggc acaaagctcc ggcaatatgg acaagatgat ggcgatggtt
    22501 gatcgctgcc tgtcggaata cgaccagaac ggctggactg tgccgcacct gcacaacaac
    22561 gacgatatca acatgctgga taagctgctg aaataacgca gcaggaggtt aagatgcaat
    22621 tagatgaaca acgcctgcgc tttcatgacg cgatggccag cctgtcggca gcggtaaata
    22681 ttatcaccac cgagggcgac gccggacaat gcgggattac ggcaacggct gtctgctcgg
    22741 tcacggatac accaccgtcg ctgatggtgt gcattaacgc caacagtgcg atgaacccgg
    22801 tttttcaggg caacggcaag ttgtgcgtca acgtcctcaa ccatgagcag gaactgatgg
    22861 cgcgccactt cgcgggcatg acaggcatgg cgatggaaga gcgttttagc ctctcatgct
    22921 ggcaaaaagg tccgctggcg cagccggtgc taaaaggttc gctggccagt cttgaaggag
    22981 agatccgcga tgtgcaggcg attggcacac atctggtgta tctggtggag attaaaaaca
    23041 tcatcctcag tgcagaaggt cacggactta tttactttaa acgccgtttc catccggtga
    23101 tgctggaaat ggaagctgcg atttaagctc cagccccttc acgcattcgt cgaaggggca
    23161 tttttattgt gatctcaatc acatatctac ccgatgaata attcttgccc aaccgctcac
    23221 cgccattcct gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg
    23281 cgcacgtatc tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa
    23341 cgcaaaccta gaacggcttc ggcctatgat taacaaatta ctctatggat tttggtttgc
    23401 aggaaggcag caagtgagtg aatccccggg agcttacaaa agtaagtgtc agaggtgaac
    23461 gaacgcagct gccgcacctg taagccaaaa gacgacgagt aaaatgccag gttttactat
    23521 ggatacgaaa aaactattca agcacatacc ctgggtgatt ctcggaatca tcggtgcatt
    23581 ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt
    23641 cgcctctgtg tcggtgtatc tggtggcgta tcgctactac agtctgtaca tcgcccagaa
    23701 ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa
    23761 ctacgttccg accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc
    23821 tggtccgctg gtgggaccgg ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg
    23881 gctgctggcg ggggtagtgc tggccggtgc ggtacaggac tttatggtgc tgtttatctc
    23941 ctctcgccgt aacggcgcat ctctcggtga gatgatcaaa gaagagatgg ggccagtacc
    24001 ggggactatc gcgctgtttg gctgtttctt aatcatgatc atcatcctcg ccgttctggc
    24061 gctgattgtg gttaaagccc tggccgaaag tccgtggggt gtcttcaccg tttgctcaac
    24121 cgtaccgatt gcgctgttta tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg
    24181 tgaagtctct gttattggta tcgtgctgct ggttgcctct atctacttcg gtggcgtgat
    24241 tgctcacgat ccgtactggg gtccggcact gacctttaaa gacaccacca ttaccttcgc
    24301 gctgattggc tatgcgtttg tttccgcact gctgccggtg tggctgatcc tcgcaccgcg
    24361 cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt
    24421 ggtgctgaac ccggaactga aaatgcctgc gatgacccag tacattgacg gtactggccc
    24481 gctgtggaaa ggcgctctgt tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc
    24541 tggcttccac gcgctgatct cttccggtac gacgccgaaa ctgctggcta acgaaaccga
    24601 cgcgcgtttc attggctacg gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct
    24661 ggttgcagcg tccatcatcg aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg
    24721 ccttggcatc accatgccta acctgcatga aatgggtggc gagaacgcgc cgatcatcat
    24781 ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt
    24841 gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa
    24901 ccgtgcaggt ggtgcgccga cgctggcggt aggtatcgct cacgtgttcc acaaagtgct
    24961 gccgatggct gacatgggct tctggtatca cttcggtatt ctgttcgaag ccctgttcat
    25021 cctgaccgcg ctggatgcgg gtacccgttc tggccgcttt atgctgcaag acctgctggg
    25081 taacttcatc ccgttcctga aaaaaaccga ctctctggtt gccggtatca tcggtactgc
    25141 gggctgtgtg ggtctgtggg gctacctgct gtatcagggc gtggtcgatc cgctgggcgg
    25201 cgttaagagc ctgtggccgc tgttcggtat ctccaaccag atgctggcag ccgtagcgct
    25261 ggtactgggc accgttgtgc tgattaagat gaagcgcacc caatacatct gggtaactgt
    25321 tgttccggct gtatggctgc ttatctgcac cacctgggcg ctgggtctga aactgttcag
    25381 caccaacccg cagatggaag gcttcttcta catggcaagc cagtacaaag agaagattgc
    25441 taacggtact gacctgacgg cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa
    25501 ctacaccaac gcaggcctga gtattctgtt cctgattgtg gtgtacagca tcatcttcta
    25561 cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc
    25621 gtacgttcca atcccggaag gcggcgtgaa gatctcttcg caccactaac cgtgtttagc
    25681 cccgcttcgg cggggctttg ttcttatcag agtgaactat gtttggtaac ttaggacagg
    25741 caaaaaaata tctcggccag gcggcgaaga tgttgattgg tattccagac tacgacaact
    25801 acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct
    25861 tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt
    25921 aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc
    25981 aaaactaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt
    26041 gaaaacgaat tcggcgaagt ctccgttgat gaccaactga ttggcgaccg cgccacgcag
    26101 atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaggacgca
    26161 ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgatcg tctggtgatt
    26221 gaatgcaccg gcatggccga tcccggcccg attattcaga cctttttctc ccatgagatt
    26281 ttatgccagc tctacctgct ggacggcgtg attgcgctgg tggatgcggt acatgccgat
    26341 gagcagatga accagttcac catcgcccag tcccaggtgg gctacgccga ccgtattctg
    26401 ctgactaaaa ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc
    26461 aacgcccgcg caccggtcta caccgtcacc cacggcgaca tcgacctggg cctgctgttc
    26521 aacaccaacg gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt
    26581 atcgcggata aacaaaacga tatttcgtca attgtggtgg aactggatta cccggtagat
    26641 atcagcgaag tttcccgcgt gatggaaaac ctgctgctgg aatcggcgga taaactgctg
    26701 cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt ccagggcgtc
    26761 cagcgcctct acagcgccga ctgggacagg ccgtggggcg atgaaaaacc gcatagcaca
    26821 atggtgttta tcgggattca gttgccggag gaagagattc gggcggcgtt tgcgggattg
    26881 aggaagtaag ttcgggaaag atcaactttt tgagagttcc accgcaacat ta
//
LOCUS       DAJVPT010000053        26552 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVPT010000053 DAJVPT010000000
VERSION     DAJVPT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            336..629
                     /locus_tag="OQM47_002165"
     CDS             336..629
                     /locus_tag="OQM47_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1480313.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            701..1360
                     /locus_tag="OQM47_002166"
     CDS             701..1360
                     /locus_tag="OQM47_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1480314.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1452..1898
                     /locus_tag="OQM47_002167"
     CDS             1452..1898
                     /locus_tag="OQM47_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1480315.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OQM47_002168"
                     /pseudo
     CDS             complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OQM47_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3378..3797
                     /locus_tag="OQM47_002169"
     CDS             3378..3797
                     /locus_tag="OQM47_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1480316.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3797..5065
                     /gene="umuC"
                     /locus_tag="OQM47_002170"
     CDS             3797..5065
                     /gene="umuC"
                     /locus_tag="OQM47_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1480317.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OQM47_002171"
     CDS             complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OQM47_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1480318.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OQM47_002172"
     CDS             complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OQM47_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1480319.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7549..8268
                     /gene="fadR"
                     /locus_tag="OQM47_002173"
     CDS             7549..8268
                     /gene="fadR"
                     /locus_tag="OQM47_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1480320.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8320..9852)
                     /locus_tag="OQM47_002174"
     CDS             complement(8320..9852)
                     /locus_tag="OQM47_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1480321.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10182..11480
                     /gene="dadA"
                     /locus_tag="OQM47_002175"
     CDS             10182..11480
                     /gene="dadA"
                     /locus_tag="OQM47_002175"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1480322.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11490..12560
                     /gene="dadX"
                     /locus_tag="OQM47_002176"
     CDS             11490..12560
                     /gene="dadX"
                     /locus_tag="OQM47_002176"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1480323.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12728..14142)
                     /locus_tag="OQM47_002177"
                     /pseudo
     CDS             complement(12728..14142)
                     /locus_tag="OQM47_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14753..16489)
                     /locus_tag="OQM47_002178"
     CDS             complement(14753..16489)
                     /locus_tag="OQM47_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1480324.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16584..17498)
                     /gene="ldcA"
                     /locus_tag="OQM47_002179"
     CDS             complement(16584..17498)
                     /gene="ldcA"
                     /locus_tag="OQM47_002179"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1480325.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17598..18209
                     /gene="emtA"
                     /locus_tag="OQM47_002180"
     CDS             17598..18209
                     /gene="emtA"
                     /locus_tag="OQM47_002180"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1480326.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18211..18945)
                     /locus_tag="OQM47_002181"
     CDS             complement(18211..18945)
                     /locus_tag="OQM47_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1480327.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19146..19400
                     /locus_tag="OQM47_002182"
     CDS             19146..19400
                     /locus_tag="OQM47_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1480328.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19450..20910)
                     /locus_tag="OQM47_002183"
     CDS             complement(19450..20910)
                     /locus_tag="OQM47_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1480329.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20847..21404)
                     /locus_tag="OQM47_002184"
     CDS             complement(20847..21404)
                     /locus_tag="OQM47_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1480330.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21430..22284)
                     /locus_tag="OQM47_002185"
     CDS             complement(21430..22284)
                     /locus_tag="OQM47_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1480331.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22284..23093)
                     /locus_tag="OQM47_002186"
     CDS             complement(22284..23093)
                     /locus_tag="OQM47_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1480332.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23103..23861)
                     /locus_tag="OQM47_002187"
     CDS             complement(23103..23861)
                     /locus_tag="OQM47_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1480333.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23858..24838)
                     /locus_tag="OQM47_002188"
                     /pseudo
     CDS             complement(23858..24838)
                     /locus_tag="OQM47_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(24838..25860)
                     /locus_tag="OQM47_002189"
     CDS             complement(24838..25860)
                     /locus_tag="OQM47_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1480334.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26210..>26552
                     /locus_tag="OQM47_002190"
     CDS             26210..>26552
                     /locus_tag="OQM47_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480335.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6673 a         6468 c         6852 g         6559 t
ORIGIN      
        1 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
       61 gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc agaatgagta
      121 cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct tttctgcgtt
      181 cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg ggcatggact
      241 caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
      301 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt tatcgaagca
      361 gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
      421 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg ttggatgggc
      481 ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc gagcaaggtt
      541 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
      601 ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg
      661 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
      721 gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca gtaactatgc
      781 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
      841 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
      901 tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg caacggaaga
      961 gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt
     1021 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc
     1081 ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac aaaatacaac
     1141 gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg acatgatcca
     1201 taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt
     1261 tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt
     1321 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgccagaaag
     1381 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     1441 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     1501 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat aaactgatgg
     1561 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     1621 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa
     1681 cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cggttgctgg
     1741 cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa gcggcaatgc
     1801 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     1861 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgctgatgg
     1921 gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct
     1981 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     2041 aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag agaataatcg
     2101 cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc tgatactcat
     2161 tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt aaatcatcat
     2221 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     2281 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     2341 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     2401 tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga
     2461 cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga
     2521 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     2581 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     2641 tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc attgtactca
     2701 tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacact
     2761 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg
     2821 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
     2881 tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct
     2941 ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca
     3001 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
     3061 ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta
     3121 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
     3181 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
     3241 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
     3301 tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa aaacagtata
     3361 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
     3421 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgttg
     3481 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
     3541 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
     3601 atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
     3661 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
     3721 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
     3781 ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga
     3841 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
     3901 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg gcgatccctg
     3961 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
     4021 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
     4081 cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta attgtcgcga
     4141 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     4201 tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg cggcaaaaaa
     4261 atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt
     4321 aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca gcaaaaaact
     4381 ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc ggtttatccg
     4441 taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg aaccctgttt
     4501 gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga
     4561 acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg cccgggcggc
     4621 ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta ttaagacgtc
     4681 accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac tgctgacgcc
     4741 cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg ccatctggca
     4801 agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt
     4861 cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc aattgatggc
     4921 ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg
     4981 gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata caacgcgaag
     5041 ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc ccgcaggagc
     5101 gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc tgggaaatca
     5161 ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac tgcggcattt
     5221 ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca aacacttgcg
     5281 gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca caggtggcaa
     5341 atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca ccgcggaacg
     5401 cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg gcgatcgcgc
     5461 caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata cagagcacgc
     5521 aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt gccagagcag
     5581 taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa aatcgcaaca
     5641 taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat tcgctgcgcc
     5701 accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa ggcaaaccgg
     5761 gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat ttgcataaac
     5821 cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag tgtcagtacg
     5881 agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat caatggcgcg
     5941 agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc gacagagggc
     6001 agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg
     6061 ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa gacgttatcc
     6121 gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc atgttcagat
     6181 gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat caccgcgact
     6241 accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa ggctttaccg
     6301 atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac tgacaaacca
     6361 atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac gccaataatc
     6421 gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct ttgatcgtca
     6481 aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc aaaccaacgc
     6541 agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac ggtcaccggc
     6601 gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat gatcaggttc
     6661 tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc ggcatgcatc
     6721 atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg cttgtcgata
     6781 tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg atgataaata
     6841 ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc atcgaggaac
     6901 gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg
     6961 cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata gatacccgcc
     7021 accataaaca tcaataacag caagacttca agatttgccg ccacctcttc acggacgtgt
     7081 tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc ggggagtagc
     7141 gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag caaccagccc
     7201 gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat gatgagggcg
     7261 agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag
     7321 atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat gtttaaacgc
     7381 aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga aagagatgcg
     7441 gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta gttagccctc
     7501 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
     7561 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
     7621 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
     7681 cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt gaccattcaa
     7741 catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa tatccttgaa
     7801 acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
     7861 cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc cgataaagcg
     7921 caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt tgccgagctg
     7981 gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc
     8041 cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa
     8101 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
     8161 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac
     8221 cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
     8281 aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattcttgg
     8341 cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga gcatcacatc
     8401 aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat ccagcggtgc
     8461 gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
     8521 gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat
     8581 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcatc
     8641 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa actggctgat
     8701 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
     8761 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
     8821 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
     8881 ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
     8941 cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga aggtcgcaca
     9001 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
     9061 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
     9121 gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg ttttctcttc
     9181 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat actcttcgcg
     9241 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
     9301 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc
     9361 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
     9421 actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt tatgtccata
     9481 gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca tcaagtaagc
     9541 gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac cgtgcttata
     9601 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat
     9661 gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
     9721 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
     9781 caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc
     9841 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     9901 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     9961 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
    10021 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagtcagct
    10081 caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
    10141 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
    10201 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
    10261 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
    10321 tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa gcgattaaat
    10381 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag tttcagttga
    10441 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
    10501 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
    10561 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
    10621 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
    10681 tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg cacaaactga
    10741 cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
    10801 tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
    10861 tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg attaaggccg
    10921 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
    10981 ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag gaagatggtg
    11041 cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
    11101 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg ttgcaaccgc
    11161 gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
    11221 aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg gttgtcggga
    11281 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
    11341 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
    11401 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
    11461 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
    11521 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct
    11581 ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg agcgcgctcg
    11641 gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta cgtgagcgcg
    11701 gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat ctggagattt
    11761 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
    11821 atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
    11881 tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca atggcgaatg
    11941 ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat ggaatttccg
    12001 gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt tcgttgtcca
    12061 attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg cctggcatta
    12121 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc ggattacgtc
    12181 cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
    12241 tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag
    12301 gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta gtggacggcg
    12361 tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat ttaacgcctt
    12421 gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc aaaattgatg
    12481 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg gcgttacgcg
    12541 tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc ttctggttgc
    12601 gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
    12661 ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa ctcgccgcac
    12721 aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact tcagataaat
    12781 cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta acaggctgcg
    12841 agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc tccaggtcct
    12901 cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga tccataaggt
    12961 cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa aagtccaaaa
    13021 ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc gtcggtaaat
    13081 tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg ggctgcggca
    13141 cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg ggcggcagcc
    13201 gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga gtcaagttct
    13261 ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt ttatacaggt
    13321 actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta atcactctca
    13381 ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc agtaccatca
    13441 gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag tcaagcacca
    13501 gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg cagtaaaatt
    13561 caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg ctaagattga
    13621 gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct gcccggtttt
    13681 caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct tccagacttt
    13741 ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct tccaggattt
    13801 cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc cattcgcacc
    13861 atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc aacgtgatga
    13921 tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca atgatctgcc
    13981 agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc tgcaaaattt
    14041 gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg aagatggggg
    14101 aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac gtttccttga
    14161 aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag gagccacagg
    14221 acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca gaaatgccgt
    14281 gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta aagaaaatac
    14341 gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata agaaatcgct
    14401 catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt cagtgcggca
    14461 ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg gttgaccggt
    14521 aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg ctgactttct
    14581 tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc cataatttaa
    14641 ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt tattacaggc
    14701 tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa atttaagatt
    14761 cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct tctttctcgg
    14821 ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt gccgcgccta
    14881 gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct cgcccgtctt
    14941 ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga atgaagtcac
    15001 caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta ccgagcgccg
    15061 ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt cgggtgctgc
    15121 cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc tcttttggca
    15181 tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat tgataaacaa
    15241 actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc ggacgtccga
    15301 cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt ccctgcaaga
    15361 gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca ttctccagcc
    15421 ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat cccacccagc
    15481 tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca gcaaataccg
    15541 aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga atggcaatag
    15601 gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg atttgcgcca
    15661 gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc ggccgattac
    15721 ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc gccgtggtta
    15781 aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg gcaggcaggg
    15841 cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagaccgatg acaatcccga
    15901 ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt tcatgctgct
    15961 ggatcattgc aatcagggta atcgtcagaa agaccgccat tggatcatta ctgccggatt
    16021 caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc agagaaaaga
    16081 ccgctgcagc atcggtggag ccgacgatag cgccgattaa taagccttca atcaaatcaa
    16141 gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt acgcccagcg
    16201 tcgccagcga cagcgccggt cctaacgcca cacgaaagga actggcctga gtgcgcatcc
    16261 cgccgtcgag caaaataatc gccagcgcca ggttacttac catgtaggcg aaggggtaat
    16321 tatcaaacgg gataccgccg acgccatcga ctcccgccag catgccgatc gccagaaaaa
    16381 taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata ctgctggtga
    16441 caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaat ggtcggttac
    16501 tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga cagcgtttta
    16561 cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct aatcgtcaac
    16621 tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag cgtaaccgtg
    16681 cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg ggaacgcaaa
    16741 aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt gctgccgcta
    16801 aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata gagctgcaac
    16861 agcatacgtt cgacgcggaa agggtgcacg ttaatatctt ccagcaccag aataccgttc
    16921 tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag attgcctccc
    16981 cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat ggtgaaggtt
    17041 tcattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc atccgcacca
    17101 aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc cagaagaccg
    17161 cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg ttgttggcga
    17221 gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta accgccgcgt
    17281 acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag atcttccaga
    17341 cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc gacgttattg
    17401 acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg ctgtttaatg
    17461 ctgtaacccg atggggcaat taagtgaaac agagacatgg caattccttg ctgacaacag
    17521 aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg cgattccagg
    17581 ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg ttattagcgg
    17641 gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt ccggtgcaac
    17701 gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc gtcgatccac
    17761 aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg gtgagtaaat
    17821 cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat gtttatcgcc
    17881 gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag cgtaatattt
    17941 caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc attgaagatc
    18001 cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt gcgctgctac
    18061 ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg gatgctgacg
    18121 agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc tatatctaca
    18181 aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg ctttttcccg
    18241 ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg tcgggctgac
    18301 gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc catcaatcac
    18361 tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga aaaacacccc attgccccat
    18421 gttgacttca atttgagcga agcgcatacc ttcttgtaat tcggcaggct ttgctgtttc
    18481 cagtaatgcg cccatgccgc ctaacgacaa atcatacaag cggaaacgta acgtactgtt
    18541 atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga tgcggaaata
    18601 tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtaataaatg ccggaagctg
    18661 caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac cctgagtttc
    18721 ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg cttgactgcc
    18781 gaaatccagc accagtttat ccggggttat tgccagtatt ttgctgatca gctgcccgcc
    18841 attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca cgcccaggac
    18901 ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga aaaactcctg
    18961 actgacaaac tgtctttggt ttatcggtca tagttaacaa aacttaatac aaatgcgtga
    19021 atatttttta catgttgttc ttaaatcagc cgacaaacgc ccagcatcga tcccgccctt
    19081 gcctatactt agagcgttga cgtaagcatt tcttgcgacc gattcatcga aacgagggcc
    19141 tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt atcggcaagc
    19201 taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc gggatagtcg
    19261 gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tgacggctcc atcagcggtt
    19321 ttaatttgca cagcttcctg gtggcggtgg tgggcgctat cctggtgctg ggcgtattcc
    19381 gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat gccgctatcc
    19441 gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt cttcaataaa
    19501 cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat tgacgctgaa
    19561 gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg cggctttttg
    19621 tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg ttacgcttaa
    19681 cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa gatcggttat
    19741 cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca atcctacatc
    19801 cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat ccaccgtgcc
    19861 gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg catcactcac
    19921 ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac cattccaggt
    19981 taaatccacc ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg ctatctggtt
    20041 atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg tgcggtcata
    20101 gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca cctgccagtt
    20161 aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct cgtgtttttt
    20221 cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga cattatcagc
    20281 agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc gatcgtaagc
    20341 ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg caaacgacag
    20401 cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc tgtaatcgga
    20461 ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt acatcatcag
    20521 cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt cgtttagttg
    20581 ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc agtagcgtga
    20641 tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct gcttaatgta
    20701 agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa tgccgcgttt
    20761 atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat tatttacacc
    20821 atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca aatacccaag
    20881 atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac tacggctcca
    20941 tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc tccgcccatc
    21001 tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc ccccagattg
    21061 ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg ggacataaat gggcacaccg
    21121 tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg ttcttcgttg
    21181 cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg agcgacattc
    21241 tgtttatcca aggccttcat ggtgggttga tcgagaacgg tggtcgtcgt cgatgatacc
    21301 ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg ctgggtaaag
    21361 gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata aattccgttt
    21421 aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag ctggtgccgc
    21481 gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat ttttgagtgt
    21541 cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg acggcgctat
    21601 ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta cgtctggttg
    21661 tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa ttttcttctc
    21721 gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt catgaagaaa
    21781 atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat gaattagtcc
    21841 tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg cttttcgggt
    21901 gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt gagcgagata
    21961 atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc cctgatgaag
    22021 tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt gtgaaccgtc
    22081 gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg cgacagaaac
    22141 gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct ggtgtccaat
    22201 atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta acgcatcgat
    22261 ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc ccaccagatg
    22321 agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg ataccagtga
    22381 tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac gttctgctca
    22441 aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa atcaacgtgg
    22501 gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg taattcgatc
    22561 acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct gaccagatga
    22621 gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg gcgcatatca
    22681 gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc actccagtcc
    22741 tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc ggcgcggcgg
    22801 gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt gagtttgtca
    22861 gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt ctgagcgcct
    22921 tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga ggcgcaattt
    22981 tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt gcggtgagcc
    23041 agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag catgattgtt
    23101 ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac ccgatacagg
    23161 gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac tcgtccatct
    23221 ggctctactt ggataatgct gtcggcaatg gcgttggcat ggagcgtatg gtgagtcgac
    23281 attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag ttggatctga
    23341 tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg ttgttggacg
    23401 agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt ccagcgtcgt
    23461 gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag gcgttcttgc
    23521 ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc ccatcaacac catatccagc
    23581 acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc ctgtgcgcgc
    23641 tggcgatggc tgagtgtctg cacagagagg ctatccagta ggatctcgcc ttttaataac
    23701 ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt cgcgccaagc
    23761 agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac tggatggcgg
    23821 gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct ccgcgtaaca
    23881 acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt gggatttctg
    23941 ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg ccagtacaca
    24001 ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca atgtgcggaa
    24061 cgaccagacc gatccaacca attatgcccg ctattgctac cgtgctggcg gtgattagtg
    24121 tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc gaacgggctt
    24181 cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg ggcaaggagc
    24241 ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg gacagtcccc
    24301 ccagcaatca gaaggtaatt gacggtaact gggtgtaagg atcggccagc gttttaatta
    24361 gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc agggttaaga
    24421 tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc accatcagcc
    24481 cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg attgccgcgc
    24541 aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact aagggattac
    24601 gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt aacagcgcag
    24661 caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca gactcatttt
    24721 cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc ccacttacgg
    24781 cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg attctcatta
    24841 gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga acagttgggc
    24901 gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc aggcgtgaag
    24961 tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc caaagggcaa
    25021 gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc agagaggatc
    25081 acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg gctgccagcg
    25141 caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt gcgcgacgtt
    25201 gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc ccgtttccag
    25261 ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg tggcgaatgc
    25321 ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc ggtgctcttc
    25381 gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt ttccgttaag
    25441 caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga tcaaggtttc
    25501 atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc cttccagcga
    25561 tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa tgtgctctgg
    25621 caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca ttttttctgg
    25681 cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg ctgacgatgc gtgtaatgtt
    25741 ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa aggacggtag
    25801 agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg ttatgggcat
    25861 ggtgaacatc ctgaaaagta aaatcgatat atactttcat atatatcgat aaaaatcatg
    25921 ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg caagcctgtt
    25981 gaccctctct atcattaaaa atgtatttat caggtggcta atattattta aatcatttaa
    26041 ggctaaatta tttaaatatg aatgacatta ttgttttttt atcgtccacg gttacgcgac
    26101 gttgaagtta cttgttttat aatgagcgat ttttgatatc tcgccgagaa aattatcagc
    26161 attctctcgt ttcagtgtcg tgaatatact gttaacggag gtgaaaagaa tgctgggggt
    26221 tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt gctgatagta agcagcgtaa
    26281 ccagaagtta ccgcaacaag actttgagga ttttaagaac gacgttatta atgtcattaa
    26341 caacaatgaa gatatttatt ttcttagaga gagtaattta ctgaatgcgt ttattcggac
    26401 gtgcaaaaaa agagatgata acagttacgg aaatatgata tttacgcttt attttgataa
    26461 cgatatctat gggaacagaa ttatcaaagc atcgctgaac ggcggagagt tgcagttatt
    26521 aagcacaggg tgtagcaatg ataacattct cc
//
LOCUS       DAJVPT010000054        26288 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVPT010000054 DAJVPT010000000
VERSION     DAJVPT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..558
                     /gene="tnpA"
                     /locus_tag="OQM47_002191"
                     /pseudo
     CDS             1..558
                     /gene="tnpA"
                     /locus_tag="OQM47_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            886..1968
                     /locus_tag="OQM47_002192"
     CDS             886..1968
                     /locus_tag="OQM47_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS1480336.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2457..3255)
                     /locus_tag="OQM47_002193"
                     /pseudo
     CDS             complement(2457..3255)
                     /locus_tag="OQM47_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3265..3816)
                     /gene="ybcL"
                     /locus_tag="OQM47_002194"
     CDS             complement(3265..3816)
                     /gene="ybcL"
                     /locus_tag="OQM47_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1480337.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3985..4317)
                     /gene="emrE"
                     /locus_tag="OQM47_002195"
     CDS             complement(3985..4317)
                     /gene="emrE"
                     /locus_tag="OQM47_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1480338.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4662..4976)
                     /gene="fliE"
                     /locus_tag="OQM47_002196"
     CDS             complement(4662..4976)
                     /gene="fliE"
                     /locus_tag="OQM47_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1480339.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5190..6849
                     /gene="fliF"
                     /locus_tag="OQM47_002197"
                     /pseudo
     CDS             5190..6849
                     /gene="fliF"
                     /locus_tag="OQM47_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6842..7837
                     /gene="fliG"
                     /locus_tag="OQM47_002198"
     CDS             6842..7837
                     /gene="fliG"
                     /locus_tag="OQM47_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1480340.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7830..8516
                     /gene="fliH"
                     /locus_tag="OQM47_002199"
     CDS             7830..8516
                     /gene="fliH"
                     /locus_tag="OQM47_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1480341.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8516..9889
                     /gene="fliI"
                     /locus_tag="OQM47_002200"
     CDS             8516..9889
                     /gene="fliI"
                     /locus_tag="OQM47_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1480342.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9911..10252
                     /gene="fliJ"
                     /locus_tag="OQM47_002201"
                     /pseudo
     CDS             9911..10252
                     /gene="fliJ"
                     /locus_tag="OQM47_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10249..11376
                     /gene="fliK"
                     /locus_tag="OQM47_002202"
     CDS             10249..11376
                     /gene="fliK"
                     /locus_tag="OQM47_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1480343.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11481..11945
                     /gene="fliL"
                     /locus_tag="OQM47_002203"
     CDS             11481..11945
                     /gene="fliL"
                     /locus_tag="OQM47_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1480344.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11950..12954
                     /gene="fliM"
                     /locus_tag="OQM47_002204"
     CDS             11950..12954
                     /gene="fliM"
                     /locus_tag="OQM47_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1480345.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12951..13364
                     /gene="fliN"
                     /locus_tag="OQM47_002205"
     CDS             12951..13364
                     /gene="fliN"
                     /locus_tag="OQM47_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1480346.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13367..13732
                     /gene="fliO"
                     /locus_tag="OQM47_002206"
     CDS             13367..13732
                     /gene="fliO"
                     /locus_tag="OQM47_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1480347.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13732..14497
                     /gene="fliP"
                     /locus_tag="OQM47_002207"
                     /pseudo
     CDS             13732..14497
                     /gene="fliP"
                     /locus_tag="OQM47_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            14507..14776
                     /gene="fliQ"
                     /locus_tag="OQM47_002208"
     CDS             14507..14776
                     /gene="fliQ"
                     /locus_tag="OQM47_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1480348.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14785..15570
                     /gene="fliR"
                     /locus_tag="OQM47_002209"
     CDS             14785..15570
                     /gene="fliR"
                     /locus_tag="OQM47_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1480349.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15855..16478
                     /gene="rcsA"
                     /locus_tag="OQM47_002210"
     CDS             15855..16478
                     /gene="rcsA"
                     /locus_tag="OQM47_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1480350.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16522..16710)
                     /gene="dsrB"
                     /locus_tag="OQM47_002211"
     CDS             complement(16522..16710)
                     /gene="dsrB"
                     /locus_tag="OQM47_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1480351.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16873..17100
                     /gene="yodD"
                     /locus_tag="OQM47_002212"
     CDS             16873..17100
                     /gene="yodD"
                     /locus_tag="OQM47_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1480352.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17398..18213
                     /locus_tag="OQM47_002213"
                     /pseudo
     CDS             17398..18213
                     /locus_tag="OQM47_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18210..19904)
                     /gene="yedQ"
                     /locus_tag="OQM47_002214"
                     /pseudo
     CDS             complement(18210..19904)
                     /gene="yedQ"
                     /locus_tag="OQM47_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20075..20257)
                     /locus_tag="OQM47_002215"
     CDS             complement(20075..20257)
                     /locus_tag="OQM47_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1480353.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20336..21253)
                     /locus_tag="OQM47_002216"
     CDS             complement(20336..21253)
                     /locus_tag="OQM47_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1480354.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21426..22346
                     /gene="yedA"
                     /locus_tag="OQM47_002217"
     CDS             21426..22346
                     /gene="yedA"
                     /locus_tag="OQM47_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1480355.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22335..22805)
                     /gene="vsr"
                     /locus_tag="OQM47_002218"
     CDS             complement(22335..22805)
                     /gene="vsr"
                     /locus_tag="OQM47_002218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1480356.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22786..24204)
                     /gene="dcm"
                     /locus_tag="OQM47_002219"
     CDS             complement(22786..24204)
                     /gene="dcm"
                     /locus_tag="OQM47_002219"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1480357.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24271..24811)
                     /locus_tag="OQM47_002220"
                     /pseudo
     CDS             complement(24271..24811)
                     /locus_tag="OQM47_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24851..25108)
                     /locus_tag="OQM47_002221"
     CDS             complement(24851..25108)
                     /locus_tag="OQM47_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480358.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(25109..26256)
                     /locus_tag="OQM47_002222"
     CDS             complement(join(25109..26019,26019..26256))
                     /locus_tag="OQM47_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1480359.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         6617 a         6759 c         6465 g         6447 t
ORIGIN      
        1 cgacagaaaa actacgcact tacttttcaa atgtatgtgc ttattttgaa gctgaactgg
       61 ttgaaatgga tggcgaacca gatcacgtcc atctgttaat taactatccc cccaaactgg
      121 cgatatccag tctggtaaac aaacagcctc aaaggcgtat cgggtaggtt actgcgacgg
      181 gatcgaccag atattgctgc caggtattac cgcaaagatg ttttgtggcg tcccggctat
      241 tttgccagta gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag
      301 caaacacctg gtcagatgaa aaaacgagcc ttatatcccc gccctgaagg acgggctttt
      361 acggtgcact ggataagcct ggagattatc tacacctgca tggacatcat tgtacgatgc
      421 cccgtacatg ctccatcaca tcctcaacat caaagtatta gagccttgca aaccattatg
      481 cgtctcagtt tgtctcatct atcttacgca aaaaaaatag aactaatatc aagcggcgct
      541 atattgcgta ttaaataaaa ccacctcaat tacattaata aatctaaata aatatcatgc
      601 aaataatttc catggttatt tttaggctat ttaaattaaa aaaataaatt atgagtcaat
      661 cataaataac aacctttaat ataatttgac taccaaattt atattcgttc acaaaatagt
      721 catgcattgt gtgcaataga aacagttcag ataaaaatag ggatctactt cacaatttaa
      781 atgagaaact aaaacttaca tcttgaaata atctcattga ttggatgaat atttgtagtg
      841 cagtgacatc atttttaaat aatagttcaa ataaaagggc tcacgatgaa aaaattaaca
      901 gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca ggcagcagag
      961 atttacaata aagacagtaa caagctggat ctgtacggaa aagttaatgc taagcactac
     1021 ttctcttcta acgatgcaga tgatggcgac acaacttatg ttcgtctggg cttcaaaggc
     1081 gaaactcaaa tcaacgatca actgactggt ttcggtcagt gggaatatga attcaaaggc
     1141 aaccgtgctg aatctcaagg ttcttccaaa gataaaaccc gtcttgcctt cgctggcctg
     1201 aaattcggtg actacggctc catcgattat ggccgtaact acggtgtagc atacgatatc
     1261 ggtgcatgga ccgacgttct gccagaattc ggtggcgata cctggaccca aacagatgtg
     1321 ttcatgactg gtcgcaccac aggtgttgca acctatcgta acaacgactt ctttggtctg
     1381 gttgatggtc tgaactttgc tgctcagtat cagggtaaaa atgaccgcac tgacgtaact
     1441 gaagctaacg gtgatggttt cggtttctcc actacttatg agtatgaagg attcggtgta
     1501 ggtgcaacct atgctaaatc tgaccgcact aatgatcagg ttatctacgg taacaacagc
     1561 ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg gtctgaaata tgatgcgaac
     1621 aacatctatc tggctaccac ctattctgaa acccagaaca tgactgtttt tggtaataac
     1681 catattgcca acaaagcaca aaacttcgaa gtagttgcac aatatcagtt cgacttcggt
     1741 ctgcgtccgt ccgttgctta cctgcaatct aaaggaaaag acttgggtgc gtggggtgat
     1801 caggacctga ttgaatatat tgatgtaggt gcaacctatt acttcaacaa aaatatgtcc
     1861 acttttgttg attacaaaat caacctgatt gataagagcg atttcacgaa agcatctggc
     1921 gttgctaccg atgatatcgt tgctgtagga ttggtttacc agttctaatc tgattactca
     1981 aagatatgtt gtgggaggct ttgcctcccc aacatataag tggcgctctc aagccacttt
     2041 ccttagaagc actatcttgc ttctgactat ataaattacc ctttattttc tgggacgttt
     2101 caacgcccca tttttaataa tactcagtga acaattaacg tatcaattag atttaataac
     2161 aacgatatcc gtacctaacc tgatatctaa tgtcataagc atccctttaa ttatcccctc
     2221 aacctcttgt aaatttccca ctcttgagct ccacatagcc caaacgccgg atgcaaaaaa
     2281 aatcatacgt gcatctctga aattgtgatg gaggaagtga gtatcaatta tatgtgctta
     2341 taaacgttcc cgattggcac agaagtcacc ggattgttca ggctccaata ggttgagtat
     2401 gtcgtattta ttttccaaaa taaaaatcga tgctgttgct ccttaataaa atacttttac
     2461 tctctggacg cttttcttag ctctcttggt gttatattaa aacgattatg gaacctttca
     2521 gtaaaacgag aaggacactt gtaaccattt tccctggcaa tctcgctgat gggttttact
     2581 gtcgtttgta tagcaggaca acgcattatt taacctcacg tcgtccaata tactttgaaa
     2641 acttattccc tcgcttgcca gacggcgatg caatgtggaa acagaaatgt agagatatct
     2701 cgctaccttg tttgctgtcc attttgttct aggttcggat agcattaggt tataacaacg
     2761 acttattaat gattgtttac tatctgataa aagtaaacaa ttaacatgat gtgctcctaa
     2821 tgaaagtaga acgcccattg ctaagtgttc ctgaatttgc gttgagaagc ctcgggaaat
     2881 agatgttttt agttgctccc aacaatatat taactcgggg ttctgaggta agcagaaacc
     2941 tgtcgtgtta cgaatttgat cagttatcgc ataaaatttt tgaaactttt caattagatc
     3001 aatgggataa taaagcattt ctgcaagata aagacctgct tctggataat tcacaatata
     3061 aaattcatat ccacagggaa ataatattat ctgattatta tcaacagtta gactatgcgt
     3121 ctcccaattg ataacttttt ttccctgacg gatacgacat aaagctggca taagaggctt
     3181 aaccctgtga atctcatgat gtttatgcat ccgtatttct tcaatcttta ggttagactt
     3241 atctcttgcc agcatactct taccctattt tatctcataa actggtgtta tctcagcggt
     3301 tgcaatttta ttggcattaa gcatataacc aactaacgct ccgctggaat tagaatccac
     3361 aggaatctta tcagttttta gagcccatac tttaaactgg taatgatggg gtttatctcc
     3421 tttgggagga catgctccac caaatcctgc ataaccaaaa tcatttcggc cttgaacagc
     3481 accagtcgga agttttgtac catcacgtct tcctgcatca gtgggcaaat atgttacagt
     3541 tgctggaata ttagcaacag tccagtgcca ccatccactg cctgtaggag catctggatc
     3601 atacacagtt acggcaaagc ttttggtgcc ttcaggagca ccagaccagg ttaatgaggg
     3661 cgatgtatta ccaccttcac acccaaatcc agaaaataca tgagacgttg taagttgctc
     3721 tcctgttttt atttcattac tagtgacctg aaatgctgcg gcctgcgcag aaaatgttat
     3781 gaatgccaat acacttgaaa cgataagttt tttcataaaa acctcattgt tgtgacctat
     3841 cgttatttta tttgatgtgc cttgatctca ttatgcataa aggcgcaatg atcgcgcaaa
     3901 tgcaatcata attataaagg tgccccgaac attagccata atatgcacaa atcagattcc
     3961 tgttatctca aggagaatta ttttttaatg cggtgcactt cgtgacaaca agttaatgac
     4021 taacacaccg gcacaaatca acatcatgcc tatgatagct ggcaggtcca gtcgttgacc
     4081 aaaaaatccc caggaaagca aactaatcag aacgataccg actcctgacc agatagcata
     4141 agcaatccct gtaggtatat aagccagcgt ctgagctaat aaccagaatg atgcacaata
     4201 gcaaataatt gtgccaacag agggccataa ccgtgtaaac ccctctgaaa acttcattaa
     4261 ggttgtacca atgacctctg caagtattgc gccaccaaga taaatataag ggttcatagc
     4321 atattctttc ctgttcatac tggagagaat tgtactacag tttgaacaca actcaccgtt
     4381 ttcatcctgg tgtatgtgta tccaaggggc tctggcgtgg caagagataa aggcaggaac
     4441 aataatgatc agaaggaacc ccaggctaac gaatgagttc actcttcctg ggagcaaagc
     4501 gaagacaaaa catgttatcg cgtttacgga tcgtggatgg cagaaaacaa ccaggatcag
     4561 gtatccattt taaaccagaa attgagtgag tttgccccat ccatgcccca cgctgtggga
     4621 tcggatataa ttaaacaagc atagtcaaca ggttacacaa cctacacctg catggacatc
     4681 acttcctgat acgccgccac cagcttatta cgcacctgaa tccccatttg catagaaact
     4741 gaggcttttt gcatatcggt catcacatcg tttagcgcca cgccgggttc accgagggtg
     4801 aatttttctg cctgcgtgcg ggcgactgtt tgtgtatcgc ttatgcgatc gagcgcggcg
     4861 tgcagctgcc cggcaaaact aatggtcggt tgcggcagtg attcctgcgc acgcgcactc
     4921 atcgccgtag cctgtaactg gctgataacc ccttcaatcc cctgtatcgc tgacattttc
     4981 gtctcccgga taatttctgg taacaaagcc taccagtaag tcaataagac aaaggcgcta
     5041 aatagcaaca aaaaaactgg tttattggcg gatagaaaaa acgaaagcac aaataatggg
     5101 agcgtcaatt tttcgagtgt gctgacccga gagtgagtct tgttccactt tgccaataac
     5161 gccgtccata atcagccacg aagtgcgcga tgaatgcgac tgcagcccag acaaaatctc
     5221 ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc attgattgtt accggttccg
     5281 cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa agcccccgac taccgcacat
     5341 tattcagcaa tctttccgat caggatggtg gcgcaattgt cagccaactg acgcaaatga
     5401 atattcctta ccgcttcagc gaagccagcg gcgctattga ggttccggca gataaagttc
     5461 acgaactgcg cctggcgcaa caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc
     5521 tcgatcagga aaagtttggt atcagccagt tcagcgaaca ggtgaattat cagcgggcgc
     5581 tggaaggcga gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac
     5641 atctggcaat gccgaaaccg tctttattcg tccgtgaaca aaaatcccct tctgcatcgg
     5701 tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc gctattgtac
     5761 atctggtttc cagcgcggtt gctggtctgc cgcctggaaa cgtcacgctg gtggatcagg
     5821 gcgggcatct gttaacccag tccaatacca gcgggcgcga tcttaatgac gctcagttga
     5881 aatatgccag cgatgtcgaa ggccgtattc agcagcgtat tgaagcgatc ctgtcaccta
     5941 ttgttggtaa cggtaatatt cacgcctagg tcacggcgca actggacttc gccagtaaag
     6001 aacaaacgga agaacagtat cgccctaacg gtgatgaatc tcatgcggcg cttcgttcac
     6061 gccagcttaa tgagagcgag caaagcggtt ccggttatcc gggcggcgta ccgggggcgt
     6121 tgtcgaatca accggcacct gcgaataacg cgccaatcag cacgcctccg acaaatcaaa
     6181 ataaccgcca gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc acacagcgga
     6241 atgaaaccag taactacgaa gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg
     6301 atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca gacggtaaac
     6361 cattgcctct cagcaacgaa cagatgaagc aaattgaagc tctgacccgc gaggcgatgg
     6421 gcttttctga aaaacgcggt gattcgctca atgttgttaa ctcgccgttc aatagcagtg
     6481 acgaaagcgg cggagcactg ccgttttggc aacagcaagt gtttatcgat cagttgctgg
     6541 ctgccggtcg ctggttgctg gttgctggta ctgctggtgg cgtggttgct gtggcggaaa
     6601 gcggtacgcc cgcagctaac acgtcgcgcc gaggcggtga agactgtgca gcaacaggcg
     6661 caggcccgcg aggaagtgga agatgcggtg gaagtccgtc tgagcaaaga cgaacaactc
     6721 caacaacggc gcgctaacca acgactgggg gcagaagtca tgagccagcg tatccgtgaa
     6781 atgtctgata acgatccgcg cgtggtggcg ctggtcattc gccagtggat aaataacgat
     6841 catgagtaac ctgacaggca ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga
     6901 ccgggcggca gaggtgttca agcacctctc ccagcgcgaa gtgcaaaccc tgagcgctgc
     6961 aatggcgaac gtcacgcaga tctccaacaa gcagctaacc gatgtgctgg cggagtttga
     7021 gcaagaagct gaacagtttg ctgcactgaa tatcaacgcc aacgattatc tgcgttcggt
     7081 attggtcaaa gctctgggtg aagaacgtgc cgccagcctg ctggaagata ttctcgaaac
     7141 ccgcgatacc gccagcggta ttgaaacgct caactttatg gagccgcaga gcgccgccga
     7201 tctgattcgc gatgagcatc cgcaaattat cgccaccatt ctggtgcatc tgaggcgcgc
     7261 ccaggccgcc gatattctgg cgttgttcga tgaacgtctg cgccacgacg tgatgttgcg
     7321 tatcgccacc tttggcggcg tgcagccagc cgcgctggcc gaactgaccg aagtactgaa
     7381 tggcttgctc gacggtcaga atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc
     7441 cgaaattatc aacctgatga aaacgcagca ggaagaagcc gttattaccg ccgtgcgtga
     7501 attcgacggc gagctggcgc agaaaatcat cgacgagatg ttcctgttcg agaatctggt
     7561 ggatgtcgat gatcgcagca ttcagcgtct gttgcaggaa gtggattccg aatcgctgct
     7621 gatcgcgctg aaaggggccg agcagccact gcgcgagaaa ttcctgcgca atatgtcgca
     7681 gcgtgccgcc gatattctgc gcgacgatct cgccaagcgt ggtccggtgc gtctgtcgca
     7741 ggtcgaaaac gaacagaaag cgattctgct gattgtgcgc cgtcttgccg aaactggcga
     7801 gatggtgatt ggcagcggcg aggataccta tgtctgataa tctgccgtgg aaaacctgga
     7861 cgccggacga tctcgcgcca ccaccggcag agtttgtgcc aatggtcgag tcggaagaaa
     7921 ccattattga agaggccgaa cccagccttg agcagcaact ggcgcaactg caaatgcagg
     7981 cccacgagca gggttatcag gcggggattg ccgaaggtcg ccagcaaggt cacgaacagg
     8041 gctatcagga aggactggcc caggggctgg aacaaggtct ggcagaggcg aaggctcaac
     8101 aagcgccaat tcatgcccgg atgcagcaac tggtcagcga atttcaaact acccttgatg
     8161 cgcttgatag tgtgatagct tcgcgcctga tgcagatggc gctggaggca gcacgtcagg
     8221 tgatcggtca gacaccaacg gtggataact cggcactgat caaacagatc caacagttgt
     8281 tgcagcaaga accgttattc agcgggaaac cacagctgcg cgtacacccg gatgatctgc
     8341 aacgtgtgga tgatatgctc ggcgcaactt taagtttgca tggctggcgc ttgcggggcg
     8401 atcccaccct ccaccctggc ggatgtaaag tctccgccga tgaaggcgat ctcgacgcca
     8461 gtgtcgccac tcgctggcaa gaactctgcc gtctggcagc accaggagtg gtgtaatgac
     8521 cacgcgcctg actcgctggc taaccacgct ggataacttt gaagccaaaa tggcgcagtt
     8581 gcctgcggta cgtcgctacg ggcgattaac ccgcgctacc gggctggtgc tggaagccac
     8641 cggattacaa ttgccgctcg gcgcaacctg tgtcattgag cgccagaacg gcagcgaaac
     8701 gcacgaagta gaaagcgaag tcgttggctt taacggtcaa cggctgtttt taatgccgct
     8761 ggaggaagtg gaaggtgtcc tgcccggcgc gcgtgtatat gccaaaaaca tttcggcaga
     8821 agggctgcaa agcggcaagc agttgccgct cggtccggcg ttattaggtc gtgttctgga
     8881 cggtagcggt aaaccgctcg atggcctgcc cttccccgat acgacggaaa ccggtgcgct
     8941 gattaccccg ccatttaacc cgttgcaacg tacaccgatt gaacatgtgc tggacaccgg
     9001 cgtgcgccca atcaatgccc tgcttaccgt cggacgcggg cagcgtatgg ggctgtttgc
     9061 cgggtcgggc gttggtaaaa gtgtgctgct ggggatgatg gcacgctaca cccgcgccga
     9121 tgtcattgtc gtgggtttga ttggtgaacg tgggcgcgaa gtaaaagatt ttattgagaa
     9181 tatcctcggt gccgaaggac gtgcacgctc agtggtgatt gccgctccgg cggatgtttc
     9241 tccgctcctg cgaatgcaag gtgccgccta tgccacgcgc attgccgaag attttcgcga
     9301 tcgtggtcag catgtgttgc tgattatgga ctccctcacc cgctacgcga tggcccagcg
     9361 tgaaattgcg ctggcgattg gcgaaccacc tgccactaaa ggttatccac cgtcggtgtt
     9421 tgccaaatta ccggcactgg tcgagcgtgc cggtaatggc attagcggcg gcggctcgat
     9481 taccgcgttt tataccgtgc tcaccgaagg cgatgaccag caggacccca ttgctgactc
     9541 cgcgcgggca atcctcgacg gtcacattgt gctgtctcgc cgactggcgg aagccgggca
     9601 ctatccggct atcgatattg aagcgtcgat cagtcgcgca atgacggcgt tgatcagtga
     9661 gcaacattac gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc agcgtaaccg
     9721 cgatctggtt agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg ataaagccat
     9781 cgccctgtgg ccgcagctgg agggctattt gcaacaaggc atttttgaac gcgcggactg
     9841 ggaagcgtct ctccaggggc tggagcgtat tttcccgaca gtgtcataac ccaggagata
     9901 acggcagatg gcggaagaac agctcaaaat gttgatcgat tatcagaatg aatatcgcaa
     9961 taacctcaat agcgatatga gtgccgggat gaccagcaac cgctggatca actatcagca
    10021 gtttatccag acgctggaaa aagccattac tcagcatcgc cagcaactta atcagtggac
    10081 gcagaaagtt gacattgccc tgaacagctg gcgagaaaag aaacaacgtt tgcaggcctg
    10141 gcagacactg caggaacggc aatccacggc ggcactgctt gcagaaaacc gcctcgatca
    10201 gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg aaacctgaat gattcgctta
    10261 gcgcccttga ttaccgccga cgttgacacc accacattgc ctggcggcaa agccagcgat
    10321 gctgcacaag attttctcac gttgttgagc gaagcattag caggcgagac aacaactgac
    10381 aaagcggccc cccagttgct ggtagcaaca gataagccca cgacaaaagg cgagccgctg
    10441 gtcagcgata ttcttgccga tgcgcaacaa gcggatttac tgatccctgt ggatgaaaca
    10501 cttcctgtca tcaacgacga acaatccaca tcaacaccgt taaccaccgc tcagacgatg
    10561 acgttggctg cggtggctga caaaaatacg acaaaagacg aaaaagcgga tgatcttaat
    10621 gaagacgtca ccgcaagtct gagcgccctt tttgcgatgt tgccgggttt tgacaatacg
    10681 cccaaagtga ctgatgcacc gtcaaccgtg ttaccggcag agaaaccaac gctcttcaca
    10741 aaactgactt ctgcgcaact cactacagca cagcctgatg acgcccccgg cacaccagct
    10801 cagccattaa caccgctggt agcagaagcc cagagtaaag cggaagtcat cagcacgcct
    10861 tcgccggtga ccgctgacgc cagcccgcta atcactccac accagacaca gccactgccc
    10921 accgtcgccg cgcctgtttt gagtgcaccg ctgggttctc acgaatggca acaatcattg
    10981 agccagcata tttcgctgtt cacccgccag gggcaacaaa gtgcagagtt gcgtctgcac
    11041 ccacaggatt taggtgaagt gcaaatttcc ctcaaagtgg atgataacca ggcgcagatc
    11101 cagatgattt caccgcatca gcacgtacgc gccgccctgg aagcagcgct gccggtactg
    11161 cgtacgcagc tggccgaaag tggcattcag ttagggcaaa gcaacattag tggcgaaagc
    11221 tttagtggtc agcagcaggc cgcttcccag caacagcaaa gccaacgcac agcaaaccat
    11281 gaacctctgg cgggggaaga cgacgatacg cttccggttc ccgtctcttt acaagggcgt
    11341 gtaacaggta acagcggcgt tgatattttc gcctaacgtc agaggtagca ccgtaatccg
    11401 cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa ttagccgata agcagtagcg
    11461 acacaggaag accgcaacac atgactgatt acgcgataag caagaaaagc aagcgatcgc
    11521 tttggatccc gattctggta ttcattaccc tcgcggcctg tgccagcgca ggttacagct
    11581 actggcattc gcatcaggtt gccgctgacg acaaagcgca gcaacgcgtc gtgccctccc
    11641 cggtcttcta tgcgctggat acctttacgg tcaatttggg cgatgcggat cgcgtgcttt
    11701 atatcggcat aaccctgcga ctgaaagatg aagcaacccg ctcgcggctg agtgagtatt
    11761 tgccggaagt ccgtagtcgc ttgctgttac tgttttcacg tcaggatgct gccgtactgg
    11821 cgacagaaga aggcaagaaa aacctgattg ccgagattaa aaccacactt tccaccccgc
    11881 ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct gtataccgct tttattctgc
    11941 gataacgaca tgggcgatag tattctttct caagctgaaa ttgatgcgct gttgaatggt
    12001 gacagcgaag tcaaagacga accgacagcc agtattagcg gcgaaagtga cattcgtccg
    12061 tacgatccga atacccaacg acgggttgtg cgcgaacgtt tgcaggcgct ggaaatcatt
    12121 aatgagcgct ttgcccgcca ttttcgtatg gggctgttca acctgctgcg ccgtagcccg
    12181 gacattaccg tcggggccat ccgcattcag ccgtaccatg aatttgcccg caacctgccg
    12241 gtgccgacca acctgaacct aatccacctg aaaccgctac gcggcacagg gctggtagtg
    12301 ttctcgccga gtctggtgtt tatcgccgtg gataacctgt ttggcggcga tggacgcttc
    12361 ccgaccaaag tggaaggtcg cgagtttacc catactgaac agcgcgtcat caaccgtatg
    12421 ttgaagctgg cgctggaagg ctatagcgac gcctggaaag cgattaatcc gctggaagtt
    12481 gagtacgtgc gttcggaaat gcaggtggaa tttaccaata tcaccacctc gccgaacgac
    12541 attgtggtta acacgccgtt ccatgtggag attggcaacc tgaccggcga atttaatatc
    12601 tgcctgccat tcagcatgat cgagccgcta cgggaattgt tggttaaccc gccgctggaa
    12661 aactcgcgta atgaagatca gaactggcgc gataacctgg tgcgccaggt gcagcattca
    12721 cagctggaac tggtggccaa ctttgccgat atctcgctac gcctgtcgca gattttaaaa
    12781 ctgaaacccg gcgacgtcct gccgatagaa aaacccgatc gcatcatcgc ccatgttgac
    12841 ggcgtcccgg ttctgaccag tcagtacggt actctcaacg gtcagtatgc gttacggata
    12901 gaacatttga ttaacccgat tttaaattct ctgaacgagg aacagcccaa atgagtgaca
    12961 tgaataatcc ggccgatgac aacaacggcg caatggacga tctgtgggct gaagcgttga
    13021 gcgagcaaaa atcaaccagc gaaaaaagcg ctgccgacgc ggtgttccag caatttggcg
    13081 gtggtgatgt cagcggaacg ttgcaggata tcgacctgat tatggatatt ccggtcaagc
    13141 tgaccgtcga gctgggccgt acgcggatga ccatcaaaga gctgttgcgt ctgacgcaag
    13201 ggtcagtcgt ggcgctggac ggtctggcgg gcgaaccgct ggatattctg atcaacggtt
    13261 atttaatcgc ccagggcgaa gtggtggttg ttgccgataa atatggcgtg cggatcaccg
    13321 atatcattac cccgtccgag cggatgcgcc gcctgagccg ttagtgatga ataaccatgc
    13381 tactgtgcaa tcttccgcgc cggtttctgc tgagccactg ctgcaggtga gcggcgcact
    13441 catcgccatt attgccctga tcctcgctgc tgcctggctg gtaaaacggc tgggatttgc
    13501 ccctaaacgc actggcgtta acggtctgaa aattagcgcc agtgcttcgc tgggcgcgcg
    13561 tgaacgggtc gtggtggtcg atgtggaaga tgcacggctg gtgctcggcg ttaccgcagg
    13621 gcaaatcaat ctgctgcata aacttccccc ttcagcacca acggaagaga taccgcagac
    13681 cgattttcag tcggtcatga aaaatttgct taagcgtggc gggagatcct gatgcgtcgt
    13741 ttgttttctg tcgcaccggt ccttctctgg ctgattacgc ccctcgcctt cgcgcaactg
    13801 ccgggtatca ccagccagcc gctacctggc gggtatcacc agccagccgc tacctggcgg
    13861 tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt tgacgttcat
    13921 tccagcgatt ttactgatga tgaccagttt cacccgcatc attattgttt ttggtttatt
    13981 gcgtaacgcg ctgggaacgc cctccgcgcc acctaaccag gtattgctgg ggctggcact
    14041 gtttttgacc ttttttatta tgtcgccagt gatcgacaaa atttatgtcg atgcgtacca
    14101 gccattcagc gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg cgcagccgct
    14161 gcgtgagttt atgctgcgtc agacccgcga ggcagattta gggttgtttg ccagactggc
    14221 gaataccggc ccgttgcagg ggcctgaagc tgtgccgatg cgcattttgc tcccggccta
    14281 cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca tccctttttt
    14341 gattatcgac ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggttccccc
    14401 cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg gctggcaatt
    14461 gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga cacctgaatc
    14521 ggtcatgatg atgggaactg aagcgatgaa agtcgcgctg gcactggcag ccccgctgtt
    14581 gctggtagcg ctggtcacgg gccttatcat cagtattttg caggcagcca cgcagattaa
    14641 cgaaatgacg ctgtcgttta ttccgaaaat catcgccgta tttatcgcca tcattattgc
    14701 cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca ctaacctgcc
    14761 gtatatcatc gggtagcccg tacaatgatg caggagacaa gcgatcaatg gctttcctgg
    14821 ttaagcctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac cgcgccaatt
    14881 ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat gatcacgttc
    14941 gccattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg
    15001 ctggccgtac agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt
    15061 gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc atttgcgaca
    15121 tttgtcgatc ctggcagcca tctcaatatg cccgttttag cgcgcatcat ggatatgctg
    15181 gcattactgc tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat
    15241 acctttcaca ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt tctggcaccc
    15301 accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact
    15361 ctgttgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc gcaattatcc
    15421 atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta
    15481 atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa tttgctggct
    15541 gatattatta gtgaattacc attaatataa ttccgtaacg tttatcatgc taccctaagg
    15601 attatccgaa aaataatatc tgcgaacatc ttccaggata ctcctgtagc gaaatatttg
    15661 ttttaagctc actcacatat cgcaacattt actttaagac aattccaggc aaattataca
    15721 acactttacg ggatagtaag tccgcctgaa aaattgcgag agtggcgcat taggtgaccc
    15781 atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg
    15841 agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt
    15901 taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg
    15961 ttgatgacct tgccatagct tgtgattcac agcgtccttc agtggtgttt attaatgagg
    16021 actgtttcat ccacgatgct tctaacagtc agcgcatcaa gcacatcatt aatcaacatc
    16081 ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat
    16141 tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct ctcgacgata
    16201 tccttggcga tattctgaaa aaagagacaa cgataacctc gtttttaaat atgccgacgt
    16261 tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca
    16321 ttcaaatctc cgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata
    16381 ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg
    16441 ataatgtgac taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc
    16501 caccagtcag gctgaataag attactctgc tttctcaaca aagataccgt cctgatgccc
    16561 tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgcaacca ggtacattgt
    16621 gccttcacta aactcctcaa ctgccagtac cactccagga cgacgcggac cgccatccgt
    16681 tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt
    16741 agaacaattt cgtttttctg gcagcgccaa gcgcgcgcga gtgctgattt tctcgacggt
    16801 ctatacttaa gagatgccag gcggacttaa cgactggcgg caacaacaga gtaacggttg
    16861 cgaggaaaga tgatgaaaac cgcaaaagtg tacagcgata ccgcaaaacg tgaggtcagc
    16921 gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc
    16981 agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa
    17041 ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga
    17101 atgcacaata aaaaaatccc gaccctgagg gtgtcgggat gaaacttgct taagcaagaa
    17161 gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta tctgcaattt
    17221 ttttcgctga caagaaaata ttcgcgctat gaatgattaa cgctacttat gaatagaaat
    17281 gtgacgcgcg tcaccccttg gtggctctgg tgtcaacggg atcgctcccg aaaaaatcat
    17341 ctttgattat ttgtgctgtt ttgagaatca tcctggtaag gggagtactg cgcgaccatg
    17401 ttttcaattc aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt
    17461 catagttatg actggcaacc ggcagccccc tggctcagcc gtttacacga agccaatatt
    17521 cccgtcattc tctgtagcag taaaacttta gcggaaatgc tgtacttgca aaaaatgttg
    17581 gggctacaag gcttaccgct gattgcagag aatggcgcag tgatccagct tgctgaacaa
    17641 tggcaggata tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatctgc
    17701 caggttttaa atacgctacg cgagaaagaa cattttaaat tcacgacttt tgatgatgtc
    17761 gacgatgcaa ccattgccga atggacggga ttaagccgta gctaggcggc gctgacgcag
    17821 ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt
    17881 atcgctcgtc tgaacgaact gggcttgcag tttatgcagg gtgcgcgctt ctggcacgta
    17941 ctggatgcct ctgccggaaa agatcaggct gccaactgga ttatcgcgac ctatcaacaa
    18001 ttgtcaggca gacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccttta
    18061 ctggaggtaa tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtacatctg
    18121 catgatgagg atccggcccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt
    18181 gaagggctgg accatttttt ctccgcccgt taagcgttat cgctcgcgaa tacccgattg
    18241 cgcccggcct gttttgcgag gtaaagccga cggtcggcca atgactggag ttgttcaaaa
    18301 tcataatcac cggtttcctc gctgctactt acccccagcg aggcactgat gcgtatcgtt
    18361 gtactcttgg cgatcagcat ctctttttca tttaacttca ggcgaatacg ttctgcgacc
    18421 tccgcagcct gcgtcagatt cgcgcctggc agaatcacac aaaactcctc accaccgacc
    18481 cgcccggcaa cgtcctgcgc acgcaaggaa ctgctaatta atccggcagc atgagaaaga
    18541 acacggtcgc ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaagatcg
    18601 acctgaatga cagaaaaagg atgttggtgc gtctgacaca atttagcgag cggacgggct
    18661 ttttcgaaca gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca cgcctgccac
    18721 tgcaacgagc tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag
    18781 aggagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg
    18841 aaatcgccgc gcacgccttc gcttagcgta tggacgcgcg ccagcacacc gtcgaaatgg
    18901 tccagacgtt cccagctaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt
    18961 tccattgcct gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatgg
    19021 tcaggattgg aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg
    19081 aggtttttat cgatggcgtt tcttaaaaat tgctgcatag tacgcacggg aatactcatc
    19141 cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt
    19201 tcagtattgc tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt
    19261 tctcgttgcg aatggccgat aaaccagggt tgggtgacat agccgtaata acgcgttggt
    19321 acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccagcacg cgagacatac
    19381 atcgcctgtt caaccatcga cgaggagttg tgcgccagtc gcagcaagta accaacttcc
    19441 agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggaa attcccctcg
    19501 ctgactaatg catccgatac gccattgact ggcagggtgc gccgtcggtt gagttcgatt
    19561 tgccaggcgt gctcgtcgcg atgctgttcg aactcggtta cggcattacg cagagaggtg
    19621 aaatccagtg gcgccacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg
    19681 acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctag
    19741 ctggagatat aggcatcttc cagcaccacc acttcacgcc aggtgagcaa ggtggaaaaa
    19801 agcaatacca cgataaagca gagattaacg acatgaccgg gccccaggcg gcgtgcgagt
    19861 tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac
    19921 ctgctttttt atgattctgg cataacccgg caatagcgta ccgcaaaaca gcgataattt
    19981 tataatggtg gcatgataag acgcgactat cattcattgc aggcattggg acaccaggcc
    20041 ggatgtgatc cggcctggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc
    20101 cctcccccgg aacaagatcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat
    20161 accaacgaca ctcaaccata ccgctggagt atccagtgac aatcatccgc gggccgccct
    20221 ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga
    20281 aacttgttca ccttagttga agatggcgaa ttttgcataa tagccagtgc gagtattaat
    20341 gtgcctgacc gcgcattttc gctactgctt tcactcccag caccacgatg ccgccgataa
    20401 taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct
    20461 gcccggcgat atgttcaata gcgtgatgca gcggcgcaat accatgtacc acaatcccgc
    20521 cgccgacgag gaacatcgcc agcgtgccga caatcgataa tgctttcatc agccaaggcg
    20581 cgataatcaa taacccttta cctaatgcct gcatcagcgc gctagatttt tccgccagcc
    20641 aataccctag gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca
    20701 ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgcc tctgccacaa
    20761 tccccagcgt gatggcgacg atttccgcag acaaaataaa atcggtacga atcgcccctt
    20821 taattttgtc cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct
    20881 ggctctgcgc tggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta
    20941 ccccttcaaa gcagagaaac gcgccaccaa tcatcaacag tggcgtaatc gcccacggga
    21001 taaacgcact gatgatcagc gccagcggca ccagaatcac tttattgatc agcgatcctt
    21061 tcgccacgcc ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg
    21121 cattgagcga taaatcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca
    21181 tcacagagat atcgtccagc agtgtggcga tatcatcgag caacgtcagt aaactactcc
    21241 cggccaaaag cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg
    21301 gcagctctga tacttgcttc acaggtcaac atttatttaa caatcaagtg caattatatc
    21361 tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt ccgccgtgag
    21421 ggattatgcg tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca
    21481 tttggggctc aacctatttt gtcattcgga ttggcgtggg aagctggcct ccgttaatga
    21541 tggcgggcgt tcgattccag gcagccggta ttttattgct ggcatttttg ctactgcgcg
    21601 gacacaaact tcccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc
    21661 tggctgtcgg taatggcatg gtgacggttg ccgaacatca aaatgttcct tccggcatcg
    21721 ccgccgtagt gattgcaacc gtgcccctct ttaccctgtg cttcagtcgc ctgtttggca
    21781 ttaaaacgcg caaactggaa tgggtgggta ttgccattgg gcttgccgga atcatcatgc
    21841 tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct
    21901 cgattagctg ggcgtttggc tcagtttatg gctcgcgcat taccttacct gtagggatga
    21961 tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgca tcgatgattg
    22021 cgggtgaaaa actgacggcg ctcccttccc tgtcaggctt ccttgcggtc ggctatctgg
    22081 cgctgtttgg ttcgattatc gccattaacg cttatatgta tttaatccgt aatgtcagtc
    22141 cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg ctgggtacgg
    22201 gactgggtgg cgaaacgctg tcgaagattg aatggctggc gctcggcgta attgttttcg
    22261 cggtggtact ggtcacgttg ggaaaatatc tcttcccggc caaacccgtt gtcgcgccag
    22321 ttattcagga tgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca
    22381 ccttcgccgc agatccactc ttccagacgc tcggtaagcg ccgcatccgt cagcttctcg
    22441 cgcctacgta acgcacactc ccagacaatc agtacgcgcc agccgagctc ctgcaagcga
    22501 ctgatatcgc ggcgatcgcg ttcaacattt ttacctatct tctccagcca gaactcagtt
    22561 cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca gccgtgggta
    22621 aatatcacgc agcgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc
    22681 tgaacgcgaa acgcaaggcc ctgcccggtt aacaaactgg cgaggcgctt ctcaatcgcc
    22741 gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg tggccttatc gtgaacgtcg
    22801 accatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag
    22861 ttttgccacc gcggcaaaga ccggcacgac caccgagttg ccgaactggc gataggcctg
    22921 agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg
    22981 cgcgcattca cgaggcgtta accgacgtgg acgatgttgc tgattcagcg gatcgtcaaa
    23041 gtctttctca cccgtggcca tatcccaacc gcgatcgatt aaaatttccg cgccatcttt
    23101 gtaataacga gcagacagcg tacgggtgac gctttgcgga ttgttcggat aaaccattcc
    23161 ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga ggtacttcca
    23221 cagcaccggc gtcaggatat atttcgcctc aaccatcggg tccagcagct gcgccagcgt
    23281 cactcgctgt gcagggaaac attcgctgat atcacgcaga gtaaaatcgg ctttcagatt
    23341 cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca ggaaatgttt
    23401 accatcgatg attttcggat cgtccggccc gttatcttct gcatcggcaa cgtcatagcc
    23461 cagttcgtcc agcgtctgca tgatgatgcg gaacgttttt cccttatcgt gactcttcag
    23521 gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tgcgcaccac
    23581 atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt
    23641 ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc
    23701 gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttg
    23761 atgactgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata
    23821 atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg
    23881 tccaccaatc gattcaaaac cacgacggat gccgccaatt ccggcaaata gatcgataaa
    23941 gcgaaacgca taatgcggat gatgttccgg tggtttgggt aacaacgttt gcagatgggc
    24001 aaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat
    24061 tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagca ttttaacatc
    24121 ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc
    24181 ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta
    24241 acataattac tgccgtttag ggccacgcgc tcagccagcg ggtgaaaacg catctatcac
    24301 cttgtgatcg ataccttcat tctcccccgc cagttcggca ccgagcttcg ccatcaaaca
    24361 gcgccaagct caatgcccct gaaacggcaa atacgcgcgc caggccaatc gcccccaggg
    24421 cttccagccg atccgcatcc tggacgattt tagcttccgt tgttaagggg gcgatttgcg
    24481 cgctgaaact gtgagcggca atggcatgac aaacggcctc aattttctcc gccggaaatt
    24541 gcacgaactc ttcacggagc aggcgacgcg tctcttctgc cgccaggatt gaagaacgct
    24601 gccgttgcgg atgatttttc gccaggctca cgatatcgtg aaaataacag gcggttaaaa
    24661 tcaccagcat atcgacatcg tcatctgccg ccagtttctg tgccgtagcc cagacacggc
    24721 gaaaatgaca caggtcatgc gccgcgtcct gatgttgatg atggtttttc aaccagttct
    24781 cgaactatgc ctgccagtgt tgtaactcca tacgcgctcc gttgccaaag agtctcaacc
    24841 ttagcagttt ttattcatag cgtctgcaac gtgcgggacg aggtcgggtg taccatgcaa
    24901 tgccgcctaa caaggcacca accagggcca taaacagaaa tccgaccaat gctcctaacc
    24961 acttccccgc ggtagatcct aaatcacttt caaatcccga cacgggggca tcggggatcc
    25021 ggctcatcac ccagatgtat cgcgccattg cccagacgaa atagccacac catgtataga
    25081 aagcccacaa tgaatcgcca cggataatct agacacttcc gagccgttga taatactggt
    25141 tttcatattc tgtcggtgac atctgttcgc tagaaccatg ccgacgctta ctgttataaa
    25201 acatttcgat gtaatcaaaa atatcactgc gggcttcttc ccgcgttccg tagatctttt
    25261 tctttatccg ttcacgtttc aacaactgga aaaaactttc tgcaaccgca ttatcatggc
    25321 agttaccgcg acggctcatg ctaccctcca ggccgtgtga tttcaggaac gactgccact
    25381 catggcttgt gtactgactg ccctgatccg aatgaaccag cacctgtttt tcgggattac
    25441 gccgccatac agccatcagc agtgcgttca ggacaatgtc ctttgtcatc cgggattgca
    25501 tggaccagcc gataattttg cgtgagaaca gatcaacaac aacggcaaga tacagccagc
    25561 cttcgtgggt cctgatgtag gttatgtccg ttacccaacg ctcatcagga gcatccggat
    25621 tgaactgtcg ctggagcctg ttgggtgaca cgatactggc ctcgccttta cgtgcccgcg
    25681 ggcttcggta tccgacctga gcctttattc cgacacgttt catcagtctc cagactctgt
    25741 ttactccgca ctgttgcccg ctgtcacgca gatccagatg gattttgcga taaccataga
    25801 cgcatcccga ttccagccag aactgtttaa tctgtcctgt cagtctcagg tctgcctgat
    25861 ggcgttgtga atgcggctgc tgaagccagg cgtaaaaacc actgggatga acatccagca
    25921 cccgacagag caggcgaaca ggccagcaac aggagttgtc acggataaag gcgtacctca
    25981 gtcggacagc tttgcgaagt acgccgcggc tttttttaat atgtcccgtt cgtcggtaac
    26041 ccgtttcagc tctttctgga gacggcggat ctcggcctga gcatctgact gttctttatt
    26101 agtggaagaa tccggaccgt acttctttat ccaggcataa aggctgtggg tggtgatatc
    26161 gagacgtgtt gcaacgctgg caacagaata accgcgatca acaacctgtt tgactgcttc
    26221 agttttaaac tcttcgggat aacgcttacc gctcatgggc acctctcttt aagccatctt
    26281 aaatgact
//
LOCUS       DAJVPT010000055        25809 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVPT010000055 DAJVPT010000000
VERSION     DAJVPT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM47_002223"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM47_002223"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1480360.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM47_002224"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM47_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1480361.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQM47_002225"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQM47_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1480362.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OQM47_002226"
     CDS             3807..3995
                     /locus_tag="OQM47_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1480363.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQM47_002227"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQM47_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1480364.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OQM47_002228"
     CDS             4756..5115
                     /locus_tag="OQM47_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1480365.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OQM47_002229"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OQM47_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OQM47_002230"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OQM47_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1480366.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQM47_002231"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQM47_002231"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1480367.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OQM47_002232"
     CDS             10913..11254
                     /locus_tag="OQM47_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1480368.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OQM47_002233"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OQM47_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OQM47_002234"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OQM47_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1480369.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13677..14963
                     /gene="dctA"
                     /locus_tag="OQM47_002235"
     CDS             13677..14963
                     /gene="dctA"
                     /locus_tag="OQM47_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1480370.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15184..16680
                     /locus_tag="OQM47_002236"
     CDS             15184..16680
                     /locus_tag="OQM47_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1480371.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16776..17705)
                     /gene="kdgK"
                     /locus_tag="OQM47_002237"
     CDS             complement(16776..17705)
                     /gene="kdgK"
                     /locus_tag="OQM47_002237"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1480372.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17937..18704
                     /gene="pdeH"
                     /locus_tag="OQM47_002238"
     CDS             17937..18704
                     /gene="pdeH"
                     /locus_tag="OQM47_002238"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1480373.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18774..20834
                     /locus_tag="OQM47_002239"
     CDS             18774..20834
                     /locus_tag="OQM47_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1480374.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21016..22338)
                     /locus_tag="OQM47_002240"
     CDS             complement(21016..22338)
                     /locus_tag="OQM47_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1480375.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22749..23762)
                     /gene="yhjD"
                     /locus_tag="OQM47_002241"
     CDS             complement(22749..23762)
                     /gene="yhjD"
                     /locus_tag="OQM47_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1480376.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23811..24710)
                     /locus_tag="OQM47_002242"
     CDS             complement(23811..24710)
                     /locus_tag="OQM47_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1480377.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25230..>25809
                     /locus_tag="OQM47_002243"
     CDS             25230..>25809
                     /locus_tag="OQM47_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1480378.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAA"
BASE COUNT         6151 a         6484 c         7027 g         6147 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgccaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccataa ccttacaaga cctgtggttt tactaaagga caccctatga
    13681 aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc attggtattc
    13741 tccttggcca tttctatcct gaaataggcg agcaaatgaa accgcttggc gacggcttcg
    13801 ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta acgggcattg
    13861 cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg ctttactttg
    13921 aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc gtgcagcctg
    13981 gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg gtttacgccg
    14041 atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg gcgagcgtca
    14101 ttggcgcatt tgccagcggt aacattctgc aggtgctgct gtttgccgta ctgtttggtt
    14161 ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc gaaagtttct
    14221 cgcaggtcat cttcggcatc atcaatatga tcatgcgtct ggcacctatt ggtgcgctcg
    14281 gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa ctggggcagc
    14341 tgattatctg tttctacatt acctgtatcc tgtttgtggt gctggtattg ggttcaatcg
    14401 ctaaagcgac tggtttcagt atcttcaaat ttatccgcta catccgtgaa gaactgctga
    14461 ttgtactggg gacttcatct tccgagtcgg cgctgccgcg tatgctcgac aagatggaga
    14521 aactcggctg ccgtaaatcg gtggtggggc tggtcatccc gacaggctac tcgtttaacc
    14581 ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag gccactaaca
    14641 gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt tcttctaaag
    14701 gggcggcagg ggtaacgggt agtggcttta tcgtgctggc ggcgacgctc tctgcggtgg
    14761 gccatttgcc ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt atgtcagaag
    14821 ctcgtgcgct aactaacctg gtcggtaacg gcgtagcgac cattgtcgtt gctaagtggg
    14881 tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg ccggatggca
    14941 aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt ccccgagtgc
    15001 gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga gttcaggttt
    15061 atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt gtttttaacg
    15121 tcatttagac tgaccgacaa cgggcagttt ttttattacc aggatagttg atcaggggtt
    15181 cacatgcagg gcataaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct
    15241 ggctatgtgc aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc
    15301 aacggtttac agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt
    15361 cgcctgctgg ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac
    15421 gccatccctc gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca
    15481 ttgtggcagc aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac
    15541 accacgctgt ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaagcgctc
    15601 tcttatctgg caaatgccac tggcaaattg accatcacac cagaaaccat caaccacgcg
    15661 ctgcaaagtc aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc
    15721 tatcgtctga aagggtcaac cttgttaggt cacgatcctg ccgatccgct gaaacaaccc
    15781 gttgaagcgg aaaaaattaa agatttctat cagaaatggt acaccccgga tgcaatgacg
    15841 ctactggtgg tgggaaacgt ggatgcgcgc tcggttgtcg accaaatcaa caaaacgttt
    15901 ggcgaactga aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc tccgctgcgt
    15961 gcggaagcgg tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg
    16021 gatacgccgt ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac
    16081 ctggcccgcg aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag tgacagcaaa
    16141 gacatcggtc ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac
    16201 atcgaatcac caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg
    16261 aaggttcgcg ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa
    16321 ctggagctgc agaaactgtt tgccgcctat gcacgggctg ataccgatat tctgatgggc
    16381 cagcggatgc gttcgttgca aaatcaggtc gtcgatatcg cgccggagca gtatcagaaa
    16441 ctgcggcagg atttccttaa cagcctgact gtggagatgt taaatcagga tctgcgtcag
    16501 cagttgtcga atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac
    16561 atgaaagcgt tgcaggcggc ctgggatcaa atcatggccc catctaccgc cgctgcgacc
    16621 acctctgtcg ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa
    16681 tgcctgatgg tgatgagtta gcacaaccat caggtattta tggatgagct ggatagaggg
    16741 gtaacacttt atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg
    16801 ataatcgcgc cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgccgcg
    16861 ttttccgcac tgccgcccgt caaacgtacc gccagataac cggcgctgaa agagtcgcca
    16921 gctgcggtgg tatcgatcac tttttctttc ggcagtttca ccgccggaac atcaactaac
    16981 gcttcaccag caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc
    17041 acgcccgcgt tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg
    17101 tcttcgtcgt ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ctggtacacc
    17161 tgctgtgtct cttctttgct ggcccacagg cgcggacgat agttattgtc gaaaatcact
    17221 tttccgcctt tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg
    17281 cttaagatcg ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg
    17341 caaatcgccg cagactgctc gctcgccagc cagaatttgg cggcggcttc gttacgccag
    17401 tagtagaacg tacgttcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggaagacgg
    17461 ttttccatcc gttgggtcag ggaagtatca acgttctcgc cgtgccaggc gtccagcatc
    17521 tgctggctaa aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga
    17581 tcgacctgac gggcgatata gacggaagtg ttcagggtat cgccgccgaa accgcgctta
    17641 acgtccgcgc ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg
    17701 gacatagtcg tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc
    17761 tggtcaacca tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa
    17821 gattccgttg tggagtgagg aaatttaccc cggccaggcg gtaaagttct gcccttacgc
    17881 gccgataatc tttgtcgagt ccgggcagca tcacttttaa acacaggaca tctttgatga
    17941 taaggcaggt tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac
    18001 ggcgtttttg gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg
    18061 ggcggttaat ggccgtggag ctattaacgg tggtcacgca tcccttgaac ccttcgcaac
    18121 gcctgccgcc ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga
    18181 aagagcagat tgatttgctg gcgcaaaaag ccgacttctt tgtagagcac ggcctgctgg
    18241 catcggtcaa tattgatggc cctacgctca tcgccctgcg tcagcaacca aaaatcctgc
    18301 gccagattga gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc cgtctgccga
    18361 aagattcaac ctttgcctcg atgtgtgaat ttggcccgct gtggctggat gattttggta
    18421 ccgggatggc aaatttctct gcactaagtg aagtgcgtta tgactacatc aaaatcgcgc
    18481 gagaactgtt tgtgatgctg cgtcagtcgc cggaaggccg cacactcttt tctcagcttt
    18541 tacatctaat gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa acaccggaag
    18601 agtggcgtga tgttcagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc
    18661 cggcaccgat ggaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg
    18721 ccgggctgga ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca
    18781 aggcaggcaa aataaccgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga
    18841 tcattttgat tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct
    18901 ctgcggagtt gaatcgtccg ttcgctatcc gtggcaatct gggcgtggtg tgggagcggc
    18961 aaaaacaaga aactggctgg cgcagctggg tgccgtggcc tcatgtacat gcagaagaca
    19021 tcattcttgg taatccaccg gatattcccg aagtcacgat ggtgcatttg ccgcgagtag
    19081 aggcaacgct ggcaccgctg gcgctgctga ccaaaacggt ctggctgccg tggatcaagc
    19141 tcgaaaagcc cgacgcgcgc ctgattcgcc tctctgaaaa gaacaataac tggacgttta
    19201 atcttgccaa cgatgacaac aaagacgcga atgcaaagcc gtcggcatgg tcgtttcggc
    19261 tggataatat tcttttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg
    19321 atctggaaat ttttgttgat ccgttaggca agccgctgcc attcagcgaa gttactggat
    19381 cgaaaggtaa agcggataaa gaaaaggtgg gcgattacat ttttggcctg aaggcgcagg
    19441 gacgttataa cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg ctggcgctgc
    19501 gtggcgaagg gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg
    19561 cttttgatgg cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat
    19621 tttctggcga ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc
    19681 cgccgtttga aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct
    19741 ttgattatcg cggttttaat gggcgaattg gcgatagcga tatccacggt tctctggtct
    19801 acaccaccgg aaagccacga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc
    19861 tggcggacct gggaccgttg attggcgttg attccgggaa aggggcagaa aagtcgaaac
    19921 ggtctgaaca gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg ctgccttatg
    19981 accgcttcga aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaagggcggc
    20041 gcattgagca tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa
    20101 atgctgacct gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc attgcggcga
    20161 atattcatct ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc
    20221 gactgaaact gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa
    20281 tgaacggtga cgcggaacta cgcggtagcg gtaactcggt ggcggcgctt ttaggcaaca
    20341 gtaacggcaa cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga
    20401 ttgttgggct gaatgtcggc aactacattg tcggtgcgat atttggtgac gatgaggtgc
    20461 gggtgaactg cgcggcggcg aatctgaata ttgccaacgg cgtggcgcgc ccgcagattt
    20521 ttgctttcga tactgagaac gcgttgatta atgttaccgg cacggcaagt tttgcttcgg
    20581 aacagctgga tttgactatt gatccggaga gtaaagggat tcggattatc acactgcgtt
    20641 cgccgctgta tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa gccggaccgc
    20701 tgattgcccg tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg gcggcggcgt
    20761 tactggcact gatctcacct tccgaagggg aggctaatca gtgtcggacg atattgtcgc
    20821 agatgaagaa gtgatgataa ttgccggatg tctgacatcc ggcaattaaa cagatttaga
    20881 tttttggcaa agtgtgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    20941 aaatccttgt gaattcaata tattgtatgg aaattcagtc ctgataagcg tagcgcatca
    21001 ggcttttcac acttattaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat
    21061 taacgtcaag ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagc
    21121 ctgcaaccag gctgcgatat acggcgcaac ggaagccccg agaatcgacg atacgttgta
    21181 agagaacgat gctccggtgt aacgcacttc tgtcggaaac agctctggta acagcgcacc
    21241 catcggcccg aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag
    21301 aatcgggttg ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag
    21361 cgtggtgatg atcaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac
    21421 tggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg
    21481 cggcaggcca agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat
    21541 gatgtaaaac agcgtatagg ttgccagcat aatgaacgta cccagtacgg ttacgcgaac
    21601 atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt
    21661 ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac
    21721 cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcat cggtcagtag
    21781 ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcctagctg
    21841 tgggaaggag ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc
    21901 cgcgccgccc cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag
    21961 cagtggggcg aaaataccaa tcgtggcata gcccggcagc agaccaatca ccacggtcga
    22021 aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa
    22081 atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag
    22141 cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat
    22201 aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga
    22261 ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgttcgt ggtcgagtgt
    22321 tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc
    22381 acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa
    22441 aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata
    22501 aatagtggat aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg
    22561 ggttatgagt tggagtgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga
    22621 agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg
    22681 gcctacgggg agccaggccg aataaaatcg tatgccggat aaggcgttta cgccgcatcc
    22741 ggcctatttt aaggctgcgt tttccctggc attcgcgggt cgtctttata ttcggcggtg
    22801 gcaatccacg ccgcgcaaaa cagcgtcaaa cgggcgaaga agtagaaaaa tgccatcagc
    22861 cccagcacgg aaccaaatgc tgcgccagag ggggatttca tcagcgatgg cagggtgtag
    22921 gtcatcacga ttttaatcac ttcaaaacca atagcagcca gaaatgttcc gcgaatcagg
    22981 gcttttttgc gcggacggtg gcgcggcaac cgccagaaga tccagaagaa aagcagatag
    23041 ttggcgaaga tggaaatcgc caggccaatc aatcgccacg tcggtttcag ccactcaatg
    23101 ctgttcaggt gcagggcgct aataatcatt tgctgcgccg aaccggcaac cgaggtgatc
    23161 gaaagcgtca caatcagcgc aatcaacaaa ccaatcagtg aaataaaatc acgcagatat
    23221 tttacccaga acttctcctg atcttgcggc gagcgttccc agacatcgcg cgactgggca
    23281 cgaatcgctt cacgcaggtt acccatccag ttgatgccgg aataaagtgc caccgccagg
    23341 ccgacaagcc ctacagtcgt acgctgctga acggcggtgt tgatggtgtt tttcaacgtg
    23401 gcggctagtg tcggatcgct gatgttttgc agaattttgt cgaagatatc ctgtagcaac
    23461 atcggatggg aggccagcac aaagccccct gcggcaaacg acaccatcaa aatcgggatc
    23521 atcgacaaaa atgagaaata ggtgatagcc gcaccaaact ggttacccag ccgatcgtta
    23581 aagcgttctg tcgcgcgaat aaggtgcgca ataaccggtt ggcgttggac tttttcggca
    23641 gtggtggtga cggtttccag cgcctggcta actttgccgc ctttctcgct attatccaac
    23701 tgcttaatcg gctcgtgctc cagatcctgg gtgggacgtt tgatctcgtt ttcctgcgtc
    23761 atccgctctt ttgtccttct gtcgtggtca ttcttaaaaa gtatagtcgg tcagtccagg
    23821 tgctctttca ttacgccagc cagccactgc ataaacaggt ttacacgccg ggaaagctcc
    23881 cgacgctgcg ggtaaaccag cgaaagggag agcggctcgg cacggtagcc tggcaacact
    23941 tcaataagcc gcccggcacg cagggcttcg cgcacggcaa tgcgcgggat ctgaataatc
    24001 cccagaccgg caagaccggc ggcgagatag ttttcgctac tgtttaccgt caacatgccg
    24061 ccagacttaa accactggac gccattaacg ctggcaacct caaaacctaa cggatgtaca
    24121 cccaggtgcg gtgtataacg cactattgcg tgtgaagtca gatcatcggg gctttgcgga
    24181 taaccaaagc gcgtcagata gtgcggactg gcacagttga ccatggttag tttgccgagg
    24241 ggacgggcga taacgccatc ttccggtaac gcgccagtgc gtatcacgca atcaaaacca
    24301 tcatgaagaa tatctaccgg acggttatgg ctactcagtt ccagctcaat tcccggatgc
    24361 tgatagagaa attccgacag acgcggcagt aacaggtttt tcgcgattcc tgacgggatg
    24421 tcgatgcgta atttaccgct gatactggtg gcatcctgtt gaaacagacc gtccagttcg
    24481 ctgagattac tcaacacatc ttttgcccgt tgataatagg tcatgccttc tggcgtgagt
    24541 ttgacccgtc gcgtagtgcg ctggagaagc tgggtgccaa gttgatgttc cagtgcctgt
    24601 atctggcgcg aaacacttcc ctttggcaaa gcaaagaaat ccgccgcgcg ggaaaaactt
    24661 tccagctccg cgactttgat gaacaactgc attgcgtgaa ttttatccat ttctttgacc
    24721 gattgttgtt cacagcgaaa cagtaacgcg catatcgcat tgtttattgt tttttctaac
    24781 atctaatgcc gggtttcaca atgttgtcat gacgaatgtg gcgatttctc agcaggaaaa
    24841 gggcggaata gctttatgag aaaggtgaat ccccttaaac agagcgtatt tttcagtgat
    24901 tccctttaaa gtgaatagag gacgtattgg ctatttgtgc ttatttttgt ctcattgcca
    24961 tttgttattt tggcttgtag tcgcggaatt tcatttttaa ttgcatgcgt actaaccacc
    25021 aatataagga aatcctcaaa attgttttaa atagtgccct cgacatgtaa tttcaggcgt
    25081 ttagctgtaa ctatttatat cggtaactat tttctacgcg tcatctctgt agaatggcca
    25141 ccgccaaatg gcgaattctt attttttctc gcgggtaata cccttccact acttaagtct
    25201 ttttaacgta ttcaggtgga gattcagtta tgcaaatagt catgtttgac aggcagtcaa
    25261 tatttattca tggaataaaa atcagtttac agcagcgtat tccaggagtg agtattcagg
    25321 gggtcagtca ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta
    25381 tgctcgatgg cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc
    25441 agtttcctga ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg
    25501 aagttatcca ttttaatgtg ctggccattg tgcctcgcga ttcaaccgtc gagacatttg
    25561 cgttggcggt aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga
    25621 cgccggaaaa ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa
    25681 ccatgttagc ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg
    25741 gaacggtaaa agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc
    25801 aggcggcaa
//
LOCUS       DAJVPT010000056        25218 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVPT010000056 DAJVPT010000000
VERSION     DAJVPT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1000)
                     /locus_tag="OQM47_002244"
     CDS             complement(<1..1000)
                     /locus_tag="OQM47_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCS1480379.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNF"
     gene            complement(1212..1559)
                     /gene="pspE"
                     /locus_tag="OQM47_002245"
     CDS             complement(1212..1559)
                     /gene="pspE"
                     /locus_tag="OQM47_002245"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1480380.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1602..1823)
                     /gene="pspD"
                     /locus_tag="OQM47_002246"
     CDS             complement(1602..1823)
                     /gene="pspD"
                     /locus_tag="OQM47_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1480381.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1832..2191)
                     /gene="pspC"
                     /locus_tag="OQM47_002247"
     CDS             complement(1832..2191)
                     /gene="pspC"
                     /locus_tag="OQM47_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1480382.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2191..2415)
                     /gene="pspB"
                     /locus_tag="OQM47_002248"
     CDS             complement(2191..2415)
                     /gene="pspB"
                     /locus_tag="OQM47_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1480383.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2469..3137)
                     /gene="pspA"
                     /locus_tag="OQM47_002249"
     CDS             complement(2469..3137)
                     /gene="pspA"
                     /locus_tag="OQM47_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1480384.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3304..4281
                     /gene="pspF"
                     /locus_tag="OQM47_002250"
     CDS             3304..4281
                     /gene="pspF"
                     /locus_tag="OQM47_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1480385.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4401..5666)
                     /gene="puuE"
                     /locus_tag="OQM47_002251"
     CDS             complement(4401..5666)
                     /gene="puuE"
                     /locus_tag="OQM47_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1480386.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5704..6984)
                     /gene="puuB"
                     /locus_tag="OQM47_002252"
     CDS             complement(5704..6984)
                     /gene="puuB"
                     /locus_tag="OQM47_002252"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1480387.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6986..8473)
                     /gene="puuC"
                     /locus_tag="OQM47_002253"
     CDS             complement(6986..8473)
                     /gene="puuC"
                     /locus_tag="OQM47_002253"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1480388.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8623..9180)
                     /gene="puuR"
                     /locus_tag="OQM47_002254"
     CDS             complement(8623..9180)
                     /gene="puuR"
                     /locus_tag="OQM47_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1480389.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9207..9971)
                     /gene="puuD"
                     /locus_tag="OQM47_002255"
     CDS             complement(9207..9971)
                     /gene="puuD"
                     /locus_tag="OQM47_002255"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1480390.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10183..11601
                     /gene="puuA"
                     /locus_tag="OQM47_002256"
     CDS             10183..11601
                     /gene="puuA"
                     /locus_tag="OQM47_002256"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1480391.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11750..11914
                     /locus_tag="OQM47_002257"
     CDS             11750..11914
                     /locus_tag="OQM47_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480392.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11904..13289
                     /gene="puuP"
                     /locus_tag="OQM47_002258"
     CDS             11904..13289
                     /gene="puuP"
                     /locus_tag="OQM47_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1480393.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13423..13668
                     /locus_tag="OQM47_002259"
     CDS             13423..13668
                     /locus_tag="OQM47_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1480394.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13981..15624
                     /gene="sapA"
                     /locus_tag="OQM47_002260"
     CDS             13981..15624
                     /gene="sapA"
                     /locus_tag="OQM47_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1480395.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15621..16586
                     /gene="sapB"
                     /locus_tag="OQM47_002261"
     CDS             15621..16586
                     /gene="sapB"
                     /locus_tag="OQM47_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1480396.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16573..17463
                     /gene="sapC"
                     /locus_tag="OQM47_002262"
     CDS             16573..17463
                     /gene="sapC"
                     /locus_tag="OQM47_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1480397.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17463..18455
                     /gene="sapD"
                     /locus_tag="OQM47_002263"
     CDS             17463..18455
                     /gene="sapD"
                     /locus_tag="OQM47_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1480398.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18457..19263
                     /gene="sapF"
                     /locus_tag="OQM47_002264"
     CDS             18457..19263
                     /gene="sapF"
                     /locus_tag="OQM47_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1480399.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19331..19684
                     /locus_tag="OQM47_002265"
     CDS             19331..19684
                     /locus_tag="OQM47_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1480400.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20052..20840
                     /gene="fabI"
                     /locus_tag="OQM47_002266"
     CDS             20052..20840
                     /gene="fabI"
                     /locus_tag="OQM47_002266"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1480401.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20984..22111
                     /locus_tag="OQM47_002267"
     CDS             20984..22111
                     /locus_tag="OQM47_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1480402.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22179..24113
                     /gene="rnb"
                     /locus_tag="OQM47_002268"
     CDS             22179..24113
                     /gene="rnb"
                     /locus_tag="OQM47_002268"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1480403.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24191..24294)
                     /locus_tag="OQM47_002269"
                     /pseudo
     CDS             complement(24191..24294)
                     /locus_tag="OQM47_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24348..25218
                     /locus_tag="OQM47_002270"
                     /pseudo
     CDS             24348..25218
                     /locus_tag="OQM47_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         5791 a         6593 c         6528 g         6306 t
ORIGIN      
        1 ggaagttaaa ccaggtcgtt ttactggagg gcaagctcag actttgcgcc cactggcaca
       61 gcgcctcgac gttctgtttt tgcaccgcat gtaacaccag cggcggcagc gagaactggt
      121 acaccatatg cgcttcgtca tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa
      181 cattggtttc ggtaataatc actgtacctg gtgctacgtc atcaataatc gaccgtaaca
      241 gcttgataat cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca
      301 taaagccaac ggcatccagg cggacatact cagcaccttt ctcaaggtaa cagagtaaaa
      361 catccaccat cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac
      421 taaaggtggt ccataaatgg cgcgtggaat tatcatccat ttgaaacggc gttaatagcg
      481 gtaacgcacg cggaagggtg acggcactga gatcggtttt cggatcaacc gcaataaaga
      541 aatcttcaaa acccggaagc tgttgtaaat agtttttaaa ccattcactt tttgcagaca
      601 tatggttgca gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct
      661 gccactcccc cgcttcactg gcgacctgat gataatcaat taccgaaaat ccatcatcag
      721 atgaccatgg gtaaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat
      781 ggtagaactg attaaatatg ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat
      841 aggtaattaa cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc
      901 gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac
      961 cgtagatttc gtccatgtaa tccgtaattt tctgtttcat gttgcctttc cgtggtagcg
     1021 gtcccatgag gcgagagact actccgcaga aaataaactg tcaattgagc acaagggttt
     1081 tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat
     1141 aatctatagg gatgtaaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat
     1201 ggtgaattct tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc
     1261 tcaacgtggg tatatcccat ctcgctaagg agctcttttg cctgcccgga ctggcgtccg
     1321 gcattgcaat acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct
     1381 ttcacttcct tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct
     1441 ggaacacgaa catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc
     1501 agagcaagta agcctttttt taaacatgta agcgtccttc tatggatgaa aattgccatc
     1561 cattatacgc ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt
     1621 gccagtttat tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc
     1681 agcggtcggc gagcaactga ttttatcgcc cagcccgcca cacccgccgg gccatagcgc
     1741 agtgcggtaa gaagtaccag cttgcctgct aatttgaaac caagcttcac cttttgcccg
     1801 gcctgttgcc agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg
     1861 tgaaagtatc ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca
     1921 gttcacggtc gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca
     1981 tgttgtccgg cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca
     2041 gcgccagacc gaaaaaaatc gacagcacca ccaggatacg caccagtttt accggtacat
     2101 caaaatagtt ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc
     2161 ataacttttt attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc
     2221 atcaagaata gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcaaccag
     2281 ttgcgctaat cgctgctgct cactttgcga caattcactg cgaccagaac gattgctgta
     2341 atgcagccat aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag
     2401 aaatagcgcg ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct
     2461 ggatgttatt attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg
     2521 attgcatcat cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag
     2581 ctgtggcttt ctgcttccgc ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga
     2641 gccattgctt catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt
     2701 gccgcctgat gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt
     2761 tccagctcgc caatctcttt cttcatgcgt gccagcgtat cgtccaccag cgtcacttca
     2821 tgttccaggg acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc
     2881 agatcctctc tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc
     2941 gccgacgctt gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa
     3001 gtagaacgta cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc
     3061 tgtggatctt ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag
     3121 cgagaaaaaa tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga
     3181 actgttaata caatttgcgt gccaattttt tatctttttg atttataaag atctgattga
     3241 agaatcaaca gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc
     3301 atcatggccg aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg
     3361 gaacaggttt cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc
     3421 accggtaaag agttgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg
     3481 tttatttccc ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt
     3541 cacgaagcgg gggcgtttac cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc
     3601 gacggcggta cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggaaaaa
     3661 ttattgcgtg tgattgagta cggtgaactg gagcgcgttg gcggtagcca gccgttgcag
     3721 gtgaatgtgc ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggt
     3781 acttttcgcg ctgacctgct cgaccggctg gctttcgatg tagtgcaact gccgcccctg
     3841 cgcgagcgcg aaagcgacat tatgctgatg gcagaacact ttgccattca gatgtgtcgg
     3901 gaaatcaagc tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat
     3961 tatcgttggc cgggaaatat tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc
     4021 cacggcacca gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtcca
     4081 cctgaagaag ccatcgccgt ttcagaaaat acctcgcttc cgacgctgcc gctggattta
     4141 cgcgagtttc agatgcagca ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa
     4201 tataaccaga agcgcgcggc tgaattactg gggttaactt atcatcagtt ccgcgcgttg
     4261 ttgaaaaagc accagattta gcgaagttga gcaatacaac ttgccggatg cggcgtgaat
     4321 gctttattcg gcatacataa tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca
     4381 gacattattg cgttgggcta ttaatcgctc agcgcatcct gcaaaatttt cattgccgca
     4441 tcgaattgcg catccgggat ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg
     4501 ccacaggtca gcaggagcag cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc
     4561 gccgctgacg gctcgcccgt ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg
     4621 ccgcgtaccg cagcaatggc cggaacgctt tctttggcat caatcaacgt gtttttgaga
     4681 cgctggccca gttgattcgc gcgttcgcag agtgattctt tgtcgataat attgagcacc
     4741 gcgtgcgcgg cagccaccgc cagcgggttc ccggcgtagg tgccacccaa cccgcccggc
     4801 gcgggcgcgt ccataatatt cgcgttaccg accacgcccg aaagcggcat cccgcccgcg
     4861 aggcttttcg ccatcgtcat taagtccggc ttatcggcgt aatgatccat ggcaaacagt
     4921 ttaccggtac gcgcaaagcc gctttgcact tcatcggcaa tcatcacaat accgtgctcg
     4981 tcgcacaggc ggcgaatggc ggcaaccagc tcttttggcg caacgttgaa accgcactcg
     5041 ccctgcaccg gttcgaaaat aatggccgcg acctgcttcg cttcgatgtc tgatttaaac
     5101 aagcgttcga tggcgtcgag ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa
     5161 ggtacgtgat acaccgaacc agggaacggg ccgaagccga ttttgtacgg cgcaactttt
     5221 ccggtcagcg ccatggtcat atatgtacga ccgtggaagc caccgctaaa cgcaatcacg
     5281 ccaggtcgtc cggtatgggc gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg
     5341 gtagtgaaga atgcggtttt agcctgcccg ctcactgggg caagggtgtt aattttctcc
     5401 gccagcgtga cgtagctttc gtacggcaca atctgatacg ccgtgtgggt aaactgttgc
     5461 aattgctgct ccaccgccgc gaccagatca gggtggcgat gcccggtatt cagcaccgca
     5521 atgcctgcgg cgaaatcgat gtattcgttg ccctctacgt ccttcagcgt agcgttttct
     5581 gccgactggg cgaagaagtt acacatcacg ccaaccccgc gtggggtagc agaaagacga
     5641 cgctgatgga attcattgtt gctcatgttt cacacctttt gactcttcac tgttaatcgg
     5701 aaattaaacg cccagacgat cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg
     5761 cacgcgcagc gtgcgcccgc cggggaacgg gtaatgcggc agattggcga aggcatcgaa
     5821 acgctcggcg tcaccgcgca gcagttcagc aatcaaacgc ccggcaagat gggtacaggt
     5881 gacgccgtgg ccgctgtaac cctgcatgta atagatgttg gtatcaaggc ggccgaactg
     5941 cggcatgtgc gacaaggtca gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc
     6001 cttcaactga gggaaggttt ttagtagttt cggtaccaca agacgctcaa cgtcatccgg
     6061 gttgcgtgca ccatacacta cacccccgcc gtacagcagg cggttgtcgg cggtaagacg
     6121 gtagtaatca agcagatagt tacagtcttc cacacagtag tttttcggga tcagcgaacg
     6181 ggctaaatct tccgacagcc gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg
     6241 tttcgctagt tccggctcta ctttatcgcc cagatacgca ttcccggcga caatcacata
     6301 cttcgccgtc acctgaccat tggcagtccg aaccacggct ggcgtggtgt gctggatctg
     6361 cgtcacggcg gaaagctcat acacgcgccc gccgttcagg cggatggcat cggcttcacc
     6421 aatggcgagg tttagcgggt gaatatgccc accgctgtga tccagcagcg caccagtgta
     6481 gcgatcgctg gcgacctcgc ggcgaatagc gttgacgtcc agcagctcca gctgtttatt
     6541 gccgtagcgt acccagttct ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat
     6601 cgccacaaac agaccgccgg ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc
     6661 gcggatgatc tcgccaccct cgaacatcat gctgccgagc atacgggcgg tgtccatgcc
     6721 gtagcttttt tcgatcacgt cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt
     6781 gcgcccgctt gcgccaaagc cgatgcgtga ggcttcgaga accactacgt caaagcccgc
     6841 ttccgccaga tgcagcgcgg aggagagccc ggtatagccg ccgccaacca cgcaaacgtc
     6901 gcaggtgatt gactcattca gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta
     6961 gtaactgctg gtatgttcgg tcatttcagg cctccaggct tatccagatg gttttcagtt
     7021 cagtgaattt ttcaagggca tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc
     7081 cgccaaacgg cacggtcata tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt
     7141 tcaggcgtcg gctcatgcgg tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa
     7201 ggccgtactg gctgtcgttg gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga
     7261 ccaccagcac cggaccgaaa atctcttcgc ggcttaagga cgcattcggg tccacatcca
     7321 cgaagatggt cgggccgatg gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt
     7381 gccccttgcc ttcgccttca cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga
     7441 ttaaggtgcc catggtggtt gcgggatcaa gtggatggcc cggctgccag ttttgcgcct
     7501 gctgttttaa cagggctaag aattcatcgg cgatgctctc ttccagtaac aaacgcgttc
     7561 cggcgatgcg cacctggccc tggttgtaga agatgcctgc ggcggtggcg cttgccgctt
     7621 tttgcagatc cggacaatcg gcgaaaacga tgttggcgct tttgccgccc gcttccagcc
     7681 agacacgttt catgttgcta tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg
     7741 agccggtaaa ggcgatggca tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat
     7801 gaccaaaacc cgtcaccacg ttcaacacac catccggcaa gcctgcttct ttcgccagcc
     7861 ccgcgagacg aatcgcactg agcggtgatt tttcagacgg ttttagaatc acgctgtttc
     7921 ccgccgccag cgccgggccg agtttccagc aagtcagcaa cagcgggaag ttccacggca
     7981 cgatggcggc aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg
     8041 tggtcgccac ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg
     8101 ccgcgccggg aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt
     8161 ccagcagtgc cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg
     8221 ctttacgttt agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg
     8281 cgctcaccgc acggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct
     8341 gggtgaccgg atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt
     8401 taataaataa gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat
     8461 gatgaaaatt cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt
     8521 cagggcgcag agatccccgg gctgcatgcc cgtttatttc tggcgacatt ctttatggat
     8581 tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt
     8641 atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc
     8701 ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag
     8761 aacaatttca ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc
     8821 agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc
     8881 gttatgaacc agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt
     8941 aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag
     9001 accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc
     9061 tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag
     9121 cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat
     9181 atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctgc
     9241 caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat
     9301 tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact
     9361 aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt
     9421 ccatgtagag agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc
     9481 ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga
     9541 tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg
     9601 gttgccacca ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg
     9661 agtgcggcat taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca
     9721 tcgccgtttt caccatatag gtgcggctgc acattgctgg gactactagg aagataaatg
     9781 ccatcgagtt tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc
     9841 gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg
     9901 gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt
     9961 atattttcca tgctcaatct cacaaaatgg actaaattat cgccattact gcgctttaaa
    10021 tgacctttat gttcaatatt ttttcaatct agcagtggat tttcattttt gcaaactcaa
    10081 tttaacattt gacaaacatt tggtttgcat acagattcga atgatggtca ttatatttta
    10141 cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt
    10201 gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa
    10261 gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat
    10321 ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa
    10381 gggtgttatt tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa
    10441 gcaggtctgg gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc
    10501 ttaactcctt ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat
    10561 gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag
    10621 ctgcgccagc gcggattgtt cccggttgta gcggtagagc tggagttcta tttactggat
    10681 cgccagcgcg acgctgaagg ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc
    10741 aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga cgtgctcaat
    10801 gatattgatg aactggcgca gttgcagctg attccggcag atggcgcggt cgctgaggcc
    10861 tcgccgggtc agtttgaaat caacctttac catactgata acgtgctgga agcctgcgat
    10921 gatgcgctgg cactaaaacg cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc
    10981 acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat ccatatcagt
    11041 atgcaaaata atcgtggcga gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg
    11101 ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc gttgctggca
    11161 ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg
    11221 tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggtgaccg tcataatcac
    11281 cgcgtggaat atcacgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt
    11341 tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc
    11401 aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag
    11461 tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac
    11521 gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag
    11581 tggatgttga aaaacgcgta attccccttg cggggttgtt tcatcggcct gagcggagcc
    11641 aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc
    11701 ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat
    11761 taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg actggactcc
    11821 gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt
    11881 attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac
    11941 ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg
    12001 cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg
    12061 gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct
    12121 acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt
    12181 cgattaaccc gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt
    12241 tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc
    12301 cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg
    12361 tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg
    12421 tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt
    12481 cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga
    12541 ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg
    12601 acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct
    12661 ttatcgcggc gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc
    12721 cggacgccgc actgcctgaa attgcgctgt acgtcggcgg caagctcttt cagtcgattt
    12781 tcctctgcac cacgtttgtg aacacattag cgtctggcct ggcttcacat gccagcgtgt
    12841 cgcgtctgct gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg
    12901 tgcacccaaa atggcggact ccggcactca acgtcattat ggtcgggatt gtcgcgctgt
    12961 cggcgctgtt ctttgattta gtcaccgcga cagcactgat taacttcggt gctctggtgg
    13021 cgtttacctt cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa ggaatgaata
    13081 aaagctggaa ggatcacttc cactatttgc tgatgccgct ggttggcgcg ctgacggtgg
    13141 gtgtgctgtg ggttaaccta gagtcaacgt cactgacact cggtctggta tgggcttcgt
    13201 taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag
    13261 gtgacagaac gccggtgagc gaaacgtaat agccggatgc gcggcgcatt atcctgcctg
    13321 agggtaacgc gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat
    13381 catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg
    13441 ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag
    13501 gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat
    13561 gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
    13621 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc
    13681 agacgatcgg tcagtatcgt tgtccggata aagcgttcac gccgcatccg gctaacaatg
    13741 acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
    13801 agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg
    13861 acgccacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt
    13921 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt
    13981 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
    14041 gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc
    14101 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
    14161 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg
    14221 ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg
    14281 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
    14341 gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc
    14401 aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc
    14461 cgcaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg
    14521 tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag
    14581 aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg
    14641 tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt
    14701 aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg acgtctgtcg
    14761 aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt
    14821 ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg
    14881 gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc actggcactg
    14941 gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc
    15001 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat
    15061 ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg
    15121 tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt
    15181 caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt
    15241 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac
    15301 agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcacag
    15361 accaacctcg cccactggtg caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
    15421 tcgcagcagc tggcagcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag
    15481 gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
    15541 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
    15601 caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg
    15661 tcaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcact ccacacgcgc
    15721 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact
    15781 gggattttgg tgtttccagc attaacggtc aaccaattgc cgaacagtta aaagagctct
    15841 tcccggcgac gatagaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
    15901 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
    15961 tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg acactgtttt
    16021 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga
    16081 aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg
    16141 aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc
    16201 caacaactga agtgatccgc ctgatgcgta tcagcactat tgaagtgtat gaccagaact
    16261 atgtaaaagc ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
    16321 tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga
    16381 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
    16441 acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
    16501 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
    16561 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
    16621 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
    16681 gcgggactgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag
    16741 caatttctcg gttatcaatt attgccgcca tcatggtcgc gctatggcga agtttctttc
    16801 ttcctgggga ctgacgacct cggacgtgat gttttaagcc gtttactgag cggagctgcg
    16861 ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg
    16921 ggcaccttcg ccggggcgac acacggcctc cgctcagcgg tgcttaacca tattctggat
    16981 accttactgg cgatcccttc gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg
    17041 agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg
    17101 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
    17161 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg
    17221 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
    17281 ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg
    17341 gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc
    17401 gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa
    17461 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
    17521 ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
    17581 gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata
    17641 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
    17701 cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt
    17761 cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
    17821 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
    17881 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
    17941 ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
    18001 tgattgctga cgaaccgaca aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
    18061 tgctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
    18121 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa
    18181 ccgcgccgag taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc
    18241 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat actctacccg
    18301 gcgctatccc attgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt
    18361 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
    18421 cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg
    18481 cgtaatttaa gtaaaacgtt ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa
    18541 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag
    18601 aatggttcgg gtaaatccac actggcaaaa atgctggcgg gaatgataga acctaccagc
    18661 ggcgagttat tgattgacga tcatccgctg cattttggcg attattcctt ccgtagtcag
    18721 cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctca
    18781 caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa
    18841 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
    18901 catatgttgg cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc
    18961 ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcaat gcgttcgcag
    19021 ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc
    19081 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
    19141 gttgtcgagc gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa
    19201 cggctgattg ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc
    19261 taattttctg aatcctcctt ccagattatt cacctcattc tgacaagaga ttaaatcttg
    19321 tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagac gcaatctcac
    19381 tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt
    19441 tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgcgt
    19501 agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgata ccaggcgcac
    19561 tagctggctt gagtcgcagg gctggaccgt gctgcgtttc tggaataacg agattgattg
    19621 taatgaggag gcggtgctgg agattattct gcaggaactg aaccgccggt caccctcacc
    19681 ctgaaagggc gagggggccg accgagccga atagctgttg tggtgaaaac atggagacag
    19741 tgctggagaa tattcggcaa ggtctgaacc atcccagcca tcgccataaa agggttaggg
    19801 gctgtatgag cctgtttgtt gctggggtaa caatatttgc acaatatggt cccctcgccc
    19861 ctctggggag agggttaggg taaggggaaa agcgcccccc ctgccgcagc ctgctccggt
    19921 cggacctggt aactatagct actcacagcc aggttgatta taataaccgt ttatctgttc
    19981 gtactgttta ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa
    20041 ggattaaagc tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca
    20101 aactgtccat cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat
    20161 tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt
    20221 ctgacatcgt tctgcagtgc gatgttgcag aagatgccag catcgacacc atgttcgctg
    20281 aactggggaa agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg
    20341 gcgatcagct ggatggtgac tatgttaacg ccgttacccg tgaaagcttc aaaattgccc
    20401 acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc
    20461 cgggttctgc cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca
    20521 acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga
    20581 tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg
    20641 cctccggtat caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc
    20701 gccgtacagt tactattgaa gatgtgggta actctgcggc attcctgtgc tccgatctct
    20761 ctgccggtat ctccggtgaa gtagtccacg ttgacggcgg tttcagcatc gctgcaatga
    20821 acgaactcga actgaaataa tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt
    20881 tatctctgtt atacctttct gatatttgtt atcgccgatc cgtctttctc cccttcccgc
    20941 cttgcgtcag gataacgatt tcctttacga ccaaggagcg cccatggaac aacgccacat
    21001 caccggcaaa agccactggt atcatgaaac gcaatccagt actacggagt atgacgttct
    21061 gcctctggtc ccggaagccg caaaggtcag cgatcccttt ctgctcgacg tgatccttga
    21121 tgaagagacg ctggccccct tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga
    21181 gttgttccct gaccagctta ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt
    21241 gtcgacggcc ctgacggttg ctcaggtttg cggcgtccag cggttatgta actactattc
    21301 ggcgcgactt acgccgctcc ccggacctga ttcctccagg gaaagtaatc atcggttggc
    21361 acaaatcacg caatatgccc gccaactggc tagctcgcct tctattatcg acaaccgatc
    21421 gcgccagcat ctgaatgacg tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat
    21481 cattggtttt attggctttc aggcgcggac aattgcgaca tttcaggctt atctcgggca
    21541 tccggtacgc tggttacccg ggctggcgat acaaaactac gccgacgcgt cactgtttgc
    21601 tgatgaatca atacgctggc gaagcagcta tgaggtggaa aaactacctg aagagcacac
    21661 aaaaagttca actgcagaac tttgccaact ggccgaaaca ctctctctcc accctatttc
    21721 actttccctt ctcgaaaagt tgttaaacag cacacgggtc aatgcacagc cggataatca
    21781 gcttgcggcg ttgttatgcg cgcgtataaa tggcagtcct gcttgttttt ccacctgtat
    21841 ggattcatca aatgaatata aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat
    21901 taaccgatgg gctgaccgtc attctgttga gcgcgctacc gttcaggcga tacaatggct
    21961 gacccgagca cccgatcgct ttagcgccgc ccagttcagc cctttactcg aacacgaaaa
    22021 atcatcaacg cagattatta atctgctggt atggagcggg ctgtgtggct ggataaatcg
    22081 cttaaaaatc gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa
    22141 actgtcagcc gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc
    22201 tagcgcagct taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag
    22261 ccacagaaaa aggctttggc ttcctggaag tcgacgcaca aaaaagttat ttcattccgc
    22321 cgccgcagat gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa
    22381 aagaacgtga atccgcagag ccagaagaac tggttgaacc gttcctgact cgtttcgtgg
    22441 gtaaagttca gggcaaaaat gaccgtctga ccatcgttcc tgatcatcca cttttaaaag
    22501 acgccattcc ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg
    22561 cggttgccga aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga
    22621 cacaatacat cacttttggt gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc
    22681 ataatctgga aaaagaagca ccagacggcg tcgctaccga aatgctcgat gaaggtctgg
    22741 ttcgtgaaga tctgacctcg ctggattttg tcaccatcga cagtgccagc actgaagata
    22801 tggatgacgc ccttttcgct aaggcgctgc cggatgacaa acttcagctg attgtggcga
    22861 ttgccgatcc aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc
    22921 gcgcattcac caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg
    22981 acgatctctg ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc
    23041 tctccgctga tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca
    23101 aagcgaagct ggtgtatgac caggtttctg actggctgga aaataccggt gactggaagc
    23161 ctgaaagtga agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg
    23221 gcgagtggcg tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc
    23281 tcggtgaaaa aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta
    23341 tcgtcgaaga agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc
    23401 tcggttttgg catctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg
    23461 cagcgttgct gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc acgctggacg
    23521 gtttctgcaa actgcgtcgt gaactggatg cgcaaccaac tggtttcctc gacagccgca
    23581 ttcgtcgctt ccagtcattt gctgaaatta gctctgaacc cggtcctcac tttggcctcg
    23641 gtctggaagc atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc
    23701 accgtctgct gaaagcggtt atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca
    23761 ctgtccaaat ggccgaacgt cgccgtctca accggatggc agaacgtgat gttggcgact
    23821 ggttatacgc acgcttcctg aaagataaag ccgggaccgg cacccgtttc gcggcggaaa
    23881 ttgtcgatat cagccgtggc ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct
    23941 ttattccggc acctttctta cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg
    24001 gcactgtaca aattaaaggt gaaacggttt acaaagtaac tgacgttatt gacgtcacca
    24061 ttgccgaagt ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg taatctcctt
    24121 tcacggccca ttcctcatgg atgggccgtt tatttccccg ctctaccttc gttatatcca
    24181 ggcagatttt ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tggattacaa
    24241 ataaatatat aaaaaaacaa tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt
    24301 ccttttattt aaccgcggac tccgctgtta accggaggat atgcatcatg aaaaccgtta
    24361 gggagtccac aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg
    24421 aaagcagcga tgctttgcgc ttttctacca ccaaaaccac agaacctgat cgtacattgc
    24481 agttatctgc cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga
    24541 tgatgagtct gaccgtcgat gaaagcgatc tttctgtgca tctggtagga cgaaaaatca
    24601 ataaacggga atgggctggc aacgcgtctg catggcatga cacaccggcg gttgctcgtg
    24661 atttatcaca cgggctttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag
    24721 tgattctcga cagccggggg aatatccaac gcttcaatcg gttatgtgaa gattacacag
    24781 ggttgaaaga acacgacgtc attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg
    24841 aagctgcggc atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag
    24901 tcgaactgtg gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg
    24961 tccacagcgg cagtggcaaa aacgagattt ttttaatctg ttccggtacc gacattaccg
    25021 aagagcgccg cactcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac
    25081 tgccgaatcg taacgcaatg caggagttaa tcgatcacgc tattaatcag gcagataaca
    25141 ataaagttgg ggttgtgtat cttgatttgg ataattttaa aaaggtcaac gacgcctatg
    25201 ggcatttgtt tggtgacc
//
LOCUS       DAJVPT010000057        24901 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVPT010000057 DAJVPT010000000
VERSION     DAJVPT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..2346
                     /gene="kdpD"
                     /locus_tag="OQM47_002271"
                     /pseudo
     CDS             1..2346
                     /gene="kdpD"
                     /locus_tag="OQM47_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            2343..3028
                     /gene="kdpE"
                     /locus_tag="OQM47_002272"
                     /pseudo
     CDS             2343..3028
                     /gene="kdpE"
                     /locus_tag="OQM47_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            3303..3407
                     /locus_tag="OQM47_002273"
     CDS             3303..3407
                     /locus_tag="OQM47_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1480404.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            3718..5915
                     /gene="speF"
                     /locus_tag="OQM47_002274"
                     /pseudo
     CDS             3718..5915
                     /gene="speF"
                     /locus_tag="OQM47_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            5912..7231
                     /gene="potE"
                     /locus_tag="OQM47_002275"
     CDS             5912..7231
                     /gene="potE"
                     /locus_tag="OQM47_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1480405.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(7287..8927)
                     /gene="pgm"
                     /locus_tag="OQM47_002276"
     CDS             complement(7287..8927)
                     /gene="pgm"
                     /locus_tag="OQM47_002276"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1480406.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(8953..9498)
                     /gene="seqA"
                     /locus_tag="OQM47_002277"
     CDS             complement(8953..9498)
                     /gene="seqA"
                     /locus_tag="OQM47_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1480407.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            9683..10447
                     /gene="ybfF"
                     /locus_tag="OQM47_002278"
     CDS             9683..10447
                     /gene="ybfF"
                     /locus_tag="OQM47_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1480408.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            10587..10880
                     /gene="ybfE"
                     /locus_tag="OQM47_002279"
     CDS             10587..10880
                     /gene="ybfE"
                     /locus_tag="OQM47_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1480409.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            11020..11550
                     /gene="fldA"
                     /locus_tag="OQM47_002280"
     CDS             11020..11550
                     /gene="fldA"
                     /locus_tag="OQM47_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1480410.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            11760..11846
                     /gene="uof"
                     /locus_tag="OQM47_002281"
     CDS             11760..11846
                     /gene="uof"
                     /locus_tag="OQM47_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1480411.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            11839..12285
                     /gene="fur"
                     /locus_tag="OQM47_002282"
     CDS             11839..12285
                     /gene="fur"
                     /locus_tag="OQM47_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1480412.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(12369..12695)
                     /locus_tag="OQM47_002283"
     CDS             complement(12369..12695)
                     /locus_tag="OQM47_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480413.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(12732..14138)
                     /gene="chiP"
                     /locus_tag="OQM47_002284"
     CDS             complement(12732..14138)
                     /gene="chiP"
                     /locus_tag="OQM47_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1480414.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(14717..16381)
                     /gene="glnS"
                     /locus_tag="OQM47_002285"
     CDS             complement(14717..16381)
                     /gene="glnS"
                     /locus_tag="OQM47_002285"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1480415.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(16584..18530)
                     /gene="nagE"
                     /locus_tag="OQM47_002286"
     CDS             complement(16584..18530)
                     /gene="nagE"
                     /locus_tag="OQM47_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1480416.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            18863..19663
                     /gene="nagB"
                     /locus_tag="OQM47_002287"
     CDS             18863..19663
                     /gene="nagB"
                     /locus_tag="OQM47_002287"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1480417.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            19723..20871
                     /gene="nagA"
                     /locus_tag="OQM47_002288"
     CDS             19723..20871
                     /gene="nagA"
                     /locus_tag="OQM47_002288"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1480418.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            20880..22100
                     /gene="nagC"
                     /locus_tag="OQM47_002289"
                     /gene_synonym="nagR"
     CDS             20880..22100
                     /gene="nagC"
                     /locus_tag="OQM47_002289"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1480419.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            22148..22900
                     /gene="nagD"
                     /locus_tag="OQM47_002290"
                     /pseudo
     CDS             22148..22900
                     /gene="nagD"
                     /locus_tag="OQM47_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            23297..>24901
                     /gene="asnB"
                     /locus_tag="OQM47_002291"
     CDS             23297..>24901
                     /gene="asnB"
                     /locus_tag="OQM47_002291"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1480420.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAF"
BASE COUNT         6027 a         6323 c         6380 g         6171 t
ORIGIN      
        1 gcacagtaat gcgccaggtt ctcgtcatcc taaacgctgg caggatatcg aagaactgct
       61 ggaagctggc attgatgttt tcacaaccgt caacgttcag catctggaaa gtctgaatga
      121 tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga
      181 tgccgccgac gacgtggtgc tggtggactt gcccccggac gatctgcgcc agcggctgaa
      241 agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa
      301 aggcaatctg atcgccctgc gcgaactggc actgcgccgt actgccgatc gcgttgatga
      361 gcaaatgcgc gcctggcggg ggcatcctgg cgaagagaaa gtctggcaca cgcgcgacgc
      421 gatcctttta tgcatcggcc ataacaccgg cagcgaaaac tggtccgcgc ggcggcgcgg
      481 ctgacgtcac ggctggggag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat
      541 cgcttaccgg agaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg
      601 ggcgcggaga cggcaacact ttctgatcca gcggaagaga aagcggtagt gcgttatgcc
      661 cgtgaacata atctcggcaa gattattctc ggtcgcccag cctcgcgccg ctggtggcgt
      721 cgggaaacat ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc
      781 gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcatttaaa
      841 gacaagtggc gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc
      901 accttaattg ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat
      961 ctgcttggcg tggtggtggc gctattttat ggacgctggc cttcggtggt tgccaccgtc
     1021 attaatgtgg tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct
     1081 gatgtgcaat atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac
     1141 cttactgctg gtgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg
     1201 cacttatatg aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc
     1261 accagcgaac aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat
     1321 gacaacggta aattgcagcc gttaacacat ccgcaaggaa ttacgccatg ggacgatgcc
     1381 atcgcgcagt ggagttttga taaaggcctg cctgcgggcg cgggcaccga cacgttaccc
     1441 ggtgtaccgt accagatttt gccgctaaaa agcggcgaga aaacctacgg gctggtggtg
     1501 gtggagccgg ggaatctgcg ccagttgatg atcccggaac agcagcgcct gctggagact
     1561 tttacgctgt tagtcgccaa tgcccttgag cggctgacgc taaccgccag cgaagaacag
     1621 gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc tgctggcagc gctttcgcat
     1681 gatttacgca cgccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg
     1741 gcaagcgaag gatcacccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac
     1801 actacccgac tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat
     1861 ttgaagaaag agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgccggaa
     1921 ccgggtttat tgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt
     1981 gacgggccac tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg
     2041 ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg gcgaaaatct acaactggat
     2101 gtctgggata acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt
     2161 gctcgcggga ataaagagtc ggcggtgccg ggtgtcgggc ttgggttggc aatttgtcgg
     2221 gcgattgtgg atgtacacgg cggcaccatt accgcgttca accgaccgga aggtggtgcc
     2281 tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac ttgaagactt tcatgaggat
     2341 atgtgacaaa cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg
     2401 cgctggaggg cgacgggatg cgcgtctttg aggccgaaac tctgcaacgc ggcttgctgg
     2461 aagcggcaac ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg
     2521 ggattgagtt tatccgcgac ctgcgccagt ggagcccggt gccggtgatt gtgctttccg
     2581 cacgcagcga agagagcgac aagatcgccg cgctggatgc cggagcggat gattatctga
     2641 gtaagccgtt tggtattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact
     2701 ccgccactcc gccaccgccg cgcccgatcc gctggtgaaa ttttccggtg ttaccgtcga
     2761 tttagccgcc cgcgtgattc accggggtga ggaagaggtg catctcacac caattgagtt
     2821 ccgcctgctg gcggtgctgc tcaacaatgc cggaaaagta ctcacccagc gccagctcct
     2881 taaccaggtg tgggggccaa acgcggtcga acacagtcac tatttgcgta tttatatggg
     2941 acatctgcga caaaaactgg aacaggatcc ggcccggcct cgccacttca ttactgaaac
     3001 cggtattggg tatcggttta tgctttgaat tgttattcaa taaattcgct taaatagtga
     3061 ttaatttatt attaaacctg cgccgaatat tattatttgg caggtttaat ttcttaacgc
     3121 aaatataaaa cacgaaatta caacatttaa gtaacaaaac agcatctaat atggcgatat
     3181 tcataattaa tgatttatta tctggggtat gatcgttttt tgttgatctt cttcacagat
     3241 tatagccatt tcatggatag aataactcta ccttcaactg acacagcaag cggtaaaggt
     3301 aaatggaaaa taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt
     3361 ttgcccatcg taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa
     3421 gggcacccca acgtacaggt ctccctgact ttaagcatta caggttaata cctgtattcc
     3481 tcggtgctca tatactgcta acccttttaa actctaaata attcgagtcg cagcacttgc
     3541 aacttgaggt atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa
     3601 aaagcaaact actgatttat ttatcagcgg tggagctttg ctttttttcc gacgtgatcg
     3661 atttctcctt tgagaaattg aggacctgct attacctgaa ataaagagat gaataaaatg
     3721 tcagaattaa aaattgccgt tagccgttct tgcccggatt gtttttccac tcatcgtgca
     3781 tgcgtgaata ttgacgaaag taattatatt gacgttgccg ccattatttt atcagtcaat
     3841 gatgttgaac gtggaaaact cgataaaata gacgctactg gctatggcat tcctgttttt
     3901 attgcaacgg aaaatgaaga acgtgtacca gcagaatatc tgccacgtat ttctggtgtc
     3961 ttcgagcatt gcgaatcacg gaaagagttt tatggacgtc agttagaaac cgctgctagc
     4021 cattatgaaa cgcaactgcg cccgcccttc ttccgcgcac tggtcgatta tgtcaatcag
     4081 ggcaatagcg cttttgactg cccgggtcat cagggaggtg agtttttccg tcgtcatccg
     4141 gcagggaatc agtccgtgga gtacttcgga gaaacgctgt tccgttccga cttatgcaac
     4201 gcagacgtag caatgggcga tctgctgatt cacgaaggcg cgccatgcat tgcacagcaa
     4261 catgcggcaa aagtgtttaa tgccgataaa acatacttcg ttctaaatgg cacttcatct
     4321 tctaacaaag tggttttaaa cgccctgcta acaccgggtg atctggtgct gtttgatcgc
     4381 aataaccaca aatctaacca ccacggagcg ttgctacagg ctggtgcaac accggtttat
     4441 ctggaaacgg cacgtaaccc gtatggcttt atcggtggca ttgatgcgca ctgttttgaa
     4501 gaaagttacc tgcgtgagct gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt
     4561 cctttccgcc tcgctgtgat tcagttaggc acctacgacg gtacgattta taacgcccgc
     4621 caggtggtgg ataaaattgg tcatctgtgt gactacattc tgtttgactc cgcatgggtt
     4681 ggctatgagc aatttatccc gatgatggct gactgttcgc cgctgttgct ggatcttaat
     4741 gagaacgatc cgggtattct agttacgcaa tccgtacata aacagcaggc tggtttttct
     4801 caaacttcac aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg
     4861 cacaaacgca tgaacaacgc ctttatgatg cacgcctcca ccagcccgtt ctatccgctg
     4921 tttgccgcac tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg
     4981 atggattgtg tggtaaatgg cattaatgcc cgcaaactga tcctcgataa ctgtcagcat
     5041 attcgtccgt tcgtacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg
     5101 caaattgcgg ttgatctgcg cttcttccag tttgtaccag gggaacactg gcattctttt
     5161 gaaggctatg cagagaatca atactttgtc gatccttgca aactgttgct gacaacccca
     5221 ggtattgatg cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct
     5281 aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaacgc catcctcttc
     5341 ctgctgactc cggcagaaga tatggccaaa cttcagcaac ttgtgccctg ctggtacgct
     5401 tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac
     5461 agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt
     5521 tgtatgcccg ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc
     5581 cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc
     5641 tggttcgtct accggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc
     5701 cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca
     5761 gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct
     5821 atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg
     5881 cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg
     5941 cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct
     6001 gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt
     6061 aggctcaatg gcaccggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg
     6121 tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta ttgcgaacta
     6181 tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta
     6241 cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg
     6301 ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag
     6361 tagcattacc gtgtgggggg gcattattcc ggtggtaggc ctgtgcatta tcggctggtt
     6421 ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag
     6481 tgcagtaggt tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg
     6541 tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg
     6601 tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc
     6661 taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgtttac
     6721 accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct
     6781 tggctggcag ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc
     6841 taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt
     6901 gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca gtcagttcaa
     6961 cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc
     7021 attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt
     7081 tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc
     7141 catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc
     7201 acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc
     7261 gaccgctctt tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca
     7321 accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc
     7381 ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact
     7441 ttcagaccgc caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg
     7501 tcacctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt
     7561 tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc
     7621 agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc
     7681 agacacataa tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag
     7741 gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca
     7801 aaccatttga aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg
     7861 tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga
     7921 cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct
     7981 gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac
     8041 ttatcacgca gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc
     8101 gcaccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg
     8161 gtcaggttga ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc
     8221 aacggatcaa cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg
     8281 gtcagccctt ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc
     8341 gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc
     8401 ttgtcttcca ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac
     8461 ttgataccac catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc
     8521 gggccacctt ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag
     8581 ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa
     8641 atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg
     8701 ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta
     8761 aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc
     8821 gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc
     8881 aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt
     8941 gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat
     9001 cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac
     9061 ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt
     9121 ttgttcatct gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc
     9181 aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc
     9241 tcgcttttgc tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc
     9301 tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt
     9361 cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc
     9421 ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc
     9481 aacttcaatc gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag
     9541 agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga
     9601 ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca
     9661 acgcgaaaaa aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca
     9721 gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg
     9781 cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca
     9841 cggtctttca ccgagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga
     9901 taccctggat gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa
     9961 agcggtaatg gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga
    10021 tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc
    10081 ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct
    10141 taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt
    10201 taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc
    10261 atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta
    10321 ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg
    10381 tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa
    10441 tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg
    10501 cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga
    10561 ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag
    10621 atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg
    10681 aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc
    10741 gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc
    10801 gtaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc
    10861 gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt
    10921 ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc
    10981 cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt
    11041 ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt
    11101 aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac
    11161 gacattctgc tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat
    11221 gacttcttcc cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt
    11281 tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac
    11341 atcattgaac cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc
    11401 gaagcatcaa aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac
    11461 cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag
    11521 ttgcatctcg acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc
    11581 gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta
    11641 cctatacaat gtccctgtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt
    11701 ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa
    11761 tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag
    11821 taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa
    11881 gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc
    11941 agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg
    12001 gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa
    12061 ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc
    12121 gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt
    12181 gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc
    12241 gaaggcgatt gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag
    12301 aaaagccaac ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca
    12361 gctctcactt atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc
    12421 accgcctgat catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt
    12481 acactttcct ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg
    12541 ctctggcagg cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat
    12601 gcctctttca gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc
    12661 gatgccatca tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc
    12721 cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca
    12781 tcctggaaga tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat
    12841 tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc
    12901 gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga
    12961 ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg
    13021 aagcctggaa gattccagtt tttcaggtca tacatcgcac tgaagaagac cgctttttcg
    13081 ccgttagcgt tgaagtcgga acggttatcc caccagatat ccaggcggcc gttggaggaa
    13141 gcgtaggttg gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaatccag
    13201 gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc
    13261 agccaggcgg tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta
    13321 ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct
    13381 ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag
    13441 ttatttttga agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta
    13501 tcgttctgat aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac
    13561 atgtaggaga aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc
    13621 ccctgataag tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc
    13681 tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac
    13741 aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa
    13801 aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac
    13861 gcagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca
    13921 ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg
    13981 tcgcgttcac gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct
    14041 tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc
    14101 agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat
    14161 aaattgctgc accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag
    14221 aaaagtctcg ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt
    14281 ctgtaaccag aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag
    14341 gcttaagttt gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg
    14401 aaagcggtta cattttattt aattatgttt tgtgatttta aatttatttt atggataaat
    14461 tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg
    14521 ggattgtttg ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca
    14581 cgcaaattca atatattgca gagatcatgt aggcctgata agcgaagcgc atcaggcaat
    14641 ttagcgtttg aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc
    14701 ttggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc
    14761 ggttaaatac cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac
    14821 gctcaaactg gaatgcttta cccgcaaccg catctttcag cgacggttca gcaaagccct
    14881 gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt
    14941 taggcacgct gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc
    15001 tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt
    15061 cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt
    15121 cagccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt
    15181 actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg
    15241 gcacctgacg gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc
    15301 cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc attgcgcgcg
    15361 gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt
    15421 tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac
    15481 cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct
    15541 tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc
    15601 gcgagaattc gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt
    15661 acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg
    15721 catcgctgat gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct
    15781 ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg
    15841 acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca
    15901 ttttttcgaa cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac
    15961 ccggctgcgt cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt
    16021 acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg
    16081 aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg
    16141 aatcgacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact
    16201 ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat
    16261 gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtgatgtgc ttaccactgg
    16321 ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca
    16381 tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag
    16441 gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat
    16501 gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg
    16561 tgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc
    16621 acaacatggc cctgagcttt aatgagcaag ccactgaaat cgtcgatatt gctgcaaacc
    16681 accgggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc
    16741 ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc
    16801 agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg
    16861 cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg
    16921 attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg
    16981 tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata
    17041 gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct
    17101 tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg
    17161 gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca
    17221 gaagcaccca gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg
    17281 cgcagacggg taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca
    17341 atatagttgg ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta
    17401 acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc
    17461 aaactgaaca ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag
    17521 acgttctggc tggcggcagg caggttatac atcaacgcgt agtcgatagc acccgcagag
    17581 aaagagaagc cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc
    17641 agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca
    17701 gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga
    17761 cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc
    17821 atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg
    17881 ttgatgtcgc cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag
    17941 gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca
    18001 aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg
    18061 tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg
    18121 gtggcgatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata
    18181 tcggaccaac ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca
    18241 cccatgttaa tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc
    18301 gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg
    18361 gcgaagatta atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg
    18421 ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata
    18481 gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc
    18541 ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact
    18601 gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt
    18661 cgcatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca
    18721 ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt
    18781 gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac
    18841 attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg
    18901 caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg
    18961 tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt
    19021 cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga
    19081 atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt
    19141 cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga
    19201 tatcgatgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct
    19261 gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct
    19321 ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt
    19381 ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact
    19441 gctggatgcc gaagaagtga tgattatggt gctgggtagc cagaaagcac tggcgctgca
    19501 ggccgccgtt gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc
    19561 gaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag
    19621 atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg
    19681 gttccttgct cagggcaata ttttttaaaa tcggggggca gaatgtatgc attaacccag
    19741 ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc
    19801 ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg
    19861 aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta
    19921 cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc
    19981 aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg
    20041 atgaaacagg gcgtgcgcgt tatgcgtgag tacctggcaa aacatccgaa tcaggcgtta
    20101 ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat
    20161 tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt
    20221 accaaagtga ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat
    20281 gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt
    20341 ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt
    20401 cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc
    20461 gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac
    20521 aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt
    20581 tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta
    20641 agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc
    20701 gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa
    20761 aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat
    20821 tttaaaatca ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta
    20881 tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg
    20941 cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc
    21001 aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc
    21061 tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca
    21121 ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca
    21181 ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta
    21241 cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc
    21301 agcgcaagct gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg
    21361 acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag
    21421 cactggaaga acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc
    21481 tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc
    21541 atcgcggaac cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta
    21601 acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca
    21661 actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc
    21721 tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct
    21781 gcaaagccgc gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc
    21841 atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg
    21901 ctggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata
    21961 cccaagcgct gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc
    22021 gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc
    22081 agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg
    22141 gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa
    22201 cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct
    22261 ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac
    22321 tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt
    22381 cctgcgtcgc taggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga
    22441 actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga
    22501 aacacgttcc tacaactggg acatgattca taaagcagcc tatttcgtcg ctaacggtgc
    22561 acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg cttgtggcgc
    22621 gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag
    22681 cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag aaacagtgat
    22741 tgtcggcgat aacctgcgta ccgatattct ggcgggcttc caggccggtc tggagacgat
    22801 tctggtgctt tctggtgttt catcgctcga cgatatcgac agtatgccgt tccgcccaag
    22861 ctggatttac ccgtcagtcg ctgaaatcga cgttatctga ataccaccca cgtctgcgga
    22921 cgtggttggc tctgatgaca aacgccaaac tgcctgatgc gctacgctta tcaggcctac
    22981 gcagctcctg caatatattg aattttcatg cttttgtagg ccggataagg cgttcacgcc
    23041 gcatccggca tgaacaaatc gcactttatc agcaatttgg cccccgtaaa atataaccat
    23101 tcaataaaac ccgctaaaaa ttatcgattc atcaataaat acatcctcac accagaccct
    23161 ttttcaccaa tcgacaatca ccattacgtt ttttattttt gcactgccaa atagcttgcg
    23221 cacagtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa caaataccag
    23281 gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa cagacgcagt
    23341 tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg
    23401 gtccggtatt tatgccagcg ataacgccat tctcgctcac gaacgtctgt caattgttga
    23461 cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa aaccacgtac tggcggtaaa
    23521 cggtgaaatc tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt atcagttcca
    23581 gacagggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc cggaatttct
    23641 cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag atgcctacct
    23701 gattggtcgc gaccatctgg ggatcatccc actgtatatg gggtatgacg aacacggtca
    23761 gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt
    23821 cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt atcatcgcga
    23881 ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc
    23941 actggaagat tccgttaaaa gccatctgat gtctgatgtg ccttacggtg tgctactttc
    24001 tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt
    24061 ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg ctgtaggtct
    24121 gccgggttca ccggatctta aagcagccca ggaagtggca aaccatctgg gcacggtgca
    24181 tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg tgatttacca
    24241 catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg
    24301 taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt
    24361 tggcggttat ctttacttcc ataaagcgcc caacgttaaa gaactgcatg aagagacggt
    24421 gcgtaaactg ctggccttgc atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc
    24481 ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg
    24541 cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga
    24601 atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc agttctccga
    24661 tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc aggtttctga
    24721 tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccgacct caaaagaagc
    24781 gtatctgtac cgcgagatct ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt
    24841 gccgggcggt ccttccgtcg cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt
    24901 c
//
LOCUS       DAJVPT010000058        24848 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVPT010000058 DAJVPT010000000
VERSION     DAJVPT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1160)
                     /gene="yeaG"
                     /locus_tag="OQM47_002292"
     CDS             complement(<1..1160)
                     /gene="yeaG"
                     /locus_tag="OQM47_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1480421.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRV"
     gene            1596..2342
                     /gene="mipA"
                     /locus_tag="OQM47_002293"
     CDS             1596..2342
                     /gene="mipA"
                     /locus_tag="OQM47_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1480422.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2432..3193
                     /locus_tag="OQM47_002294"
     CDS             2432..3193
                     /locus_tag="OQM47_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1480423.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3244..4128)
                     /locus_tag="OQM47_002295"
     CDS             complement(3244..4128)
                     /locus_tag="OQM47_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1480424.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4212..5207)
                     /gene="gapA"
                     /locus_tag="OQM47_002296"
     CDS             complement(4212..5207)
                     /gene="gapA"
                     /locus_tag="OQM47_002296"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1480425.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5549..5962
                     /gene="msrB"
                     /locus_tag="OQM47_002297"
     CDS             5549..5962
                     /gene="msrB"
                     /locus_tag="OQM47_002297"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1480426.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6004..6276
                     /locus_tag="OQM47_002298"
     CDS             6004..6276
                     /locus_tag="OQM47_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1480427.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6646..7722
                     /locus_tag="OQM47_002299"
     CDS             6646..7722
                     /locus_tag="OQM47_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1480428.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7749..9128
                     /locus_tag="OQM47_002300"
     CDS             7749..9128
                     /locus_tag="OQM47_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480429.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9145..10190
                     /locus_tag="OQM47_002301"
                     /pseudo
     CDS             9145..10190
                     /locus_tag="OQM47_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10211..11047
                     /locus_tag="OQM47_002302"
     CDS             10211..11047
                     /locus_tag="OQM47_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1480430.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11052..11999
                     /locus_tag="OQM47_002303"
     CDS             11052..11999
                     /locus_tag="OQM47_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1480431.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12009..12989
                     /gene="ydjG"
                     /locus_tag="OQM47_002304"
     CDS             12009..12989
                     /gene="ydjG"
                     /locus_tag="OQM47_002304"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1480432.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13126..13884
                     /locus_tag="OQM47_002305"
     CDS             13126..13884
                     /locus_tag="OQM47_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1480433.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14002..15360
                     /locus_tag="OQM47_002306"
     CDS             14002..15360
                     /locus_tag="OQM47_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480434.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15453..16094)
                     /gene="pncA"
                     /locus_tag="OQM47_002307"
     CDS             complement(15453..16094)
                     /gene="pncA"
                     /locus_tag="OQM47_002307"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1480435.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16105..17121)
                     /gene="ansA"
                     /locus_tag="OQM47_002308"
     CDS             complement(16105..17121)
                     /gene="ansA"
                     /locus_tag="OQM47_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1480436.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17288..19144)
                     /gene="sppA"
                     /locus_tag="OQM47_002309"
     CDS             complement(17288..19144)
                     /gene="sppA"
                     /locus_tag="OQM47_002309"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1480437.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19305..19856
                     /locus_tag="OQM47_002310"
     CDS             19305..19856
                     /locus_tag="OQM47_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1480438.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19973..21016
                     /gene="selD"
                     /locus_tag="OQM47_002311"
     CDS             19973..21016
                     /gene="selD"
                     /locus_tag="OQM47_002311"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1480439.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21021..22982
                     /gene="topB"
                     /locus_tag="OQM47_002312"
     CDS             21021..22982
                     /gene="topB"
                     /locus_tag="OQM47_002312"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1480440.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHTPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23109..24149
                     /locus_tag="OQM47_002313"
     CDS             23109..24149
                     /locus_tag="OQM47_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1480441.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24266..>24848)
                     /locus_tag="OQM47_002314"
     CDS             complement(24266..>24848)
                     /locus_tag="OQM47_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCS1480442.1"
                     /translation="GARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRAADYAK
                     EFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEAT
                     ELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACV
                     EHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6318 a         5743 c         6426 g         6361 t
ORIGIN      
        1 acccgcatct ttgaataaat gctggagttt tctggctctt tcaggcgcga aagaatggaa
       61 aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg cgtgagtcaa ttcactgtga
      121 ttaagcaatt tctcgtagat tttgatctct tcggaaatgc gcaagcaata cggcaccttc
      181 acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt tattacggaa agtgacccat
      241 tcggacttgt tcgagtgggc gagaataatc ccgttgaacg gcagggcgga gataccttcc
      301 gtcccgttgt agttaccttc ctgagtggcg gttaacaagg gatgcagcac ttttatcggt
      361 gctttaaaca tctcaacgaa ttccatgatc ccctgattgg cgcggcacag cgcaccggaa
      421 tagccgtagg cgtccgggtc attctgcgcg tagtgttcga gtttacgaat atcgactttc
      481 ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg gttccgtttt ggcgatagca
      541 atttgttgca gaattgacgg ccagaccttc actacccgga acttagtgat atcgccacca
      601 aattcatgca ggcgttttgc cgcccatggc gacatgatgg tgccgagata acggcgagga
      661 atgccatact ctttttccag aatctgagca tcttcctgcg gattgaaaag acagaacgga
      721 tgatcgttga ccgggctacg ctcaccgttg gcgctcaata cataaatcgg tacgagctgc
      781 attaatgatt tcagtcgctc agcaagcgat gatttacccc cacccacagg ccccagcaga
      841 tacaggattt gtttcttctc ttccagcccc tgagccgcgt gtttcagata agagacaatc
      901 tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg ccggataacg tgcaatgacc
      961 cggttagaaa agagtcgaga aagtctgggg tcctgggctg tatcgaccat gacaggctca
     1021 ccgatagcca tcaatagccg ctcagccgcg ttggcataag cactgcgatc ttgccgacaa
     1081 gtggtaagaa actcctgcag tgtgaactct tcgtccttgg cagcttcata tcgctggcga
     1141 tagtgatcga atatattcat ggtatgccgt cctttcgttt tttagcacag gttaagagcc
     1201 gttcatatga atagtagagg ctcccggaag aggtaaactg aacgacgtct cttgctaaaa
     1261 cacagcaact ctcatgccat tttatcgacg atgagaacaa cgtgcccggt gatacacctt
     1321 caaaattaag cgtagatggc aaatggaaaa cttgcctgca cggctttctt aatttcaatg
     1381 gcatatcaac aactcatcca taaattttgc ataattaatg taaagaccag gctcgccagt
     1441 aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc ggcaggaaaa ttaaacagtt
     1501 actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg tattgacggg taaactttgt
     1561 cgcccgctaa acatttgttt aaggaatgat taattgtgac caaactcaaa cttctggcac
     1621 ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc tgaaggtaaa ttttccctgg
     1681 gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta cgataccgat gtttacccag
     1741 taccggtaat caactatgaa ggcgataact tctggttccg tggcttaggt ggtggttact
     1801 acctgtggaa tgacgcaacg gataaacttt caattaccgc ttactggtcg ccgctttact
     1861 tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct ggatgaccgt aagagcacca
     1921 tgatggctgg tctgtcttat gctcacttta cccagtacgg ttacctgcgt accaccctgg
     1981 ctggcgatac cctggataac agcaacggca ttgtctggga tatggcctgg ttgtatcgtt
     2041 acaccaacgg tggcctgacc gtgactccgg gtattggtgt gcagtggaac agcgaaaacc
     2101 agaacgaata ctattatggc gtatcgcgca aagagtccgc tcgcagcggt ctgcgtggct
     2161 ataacccgaa tgacagctgg agcccttacc ttgagctgag cgccagctac aacttcctcg
     2221 gcgactggag tgtttacggt accgcgcgct acacccgtct gtctgatgaa gttactgaca
     2281 gcccgatggt ggataaatcc tggactggcc tgatttctac cgggatcacc tacaaattct
     2341 gataatgcat ctttccaggg cattttgtgc ataaaaacag ggcgtcacgc cctgttttgc
     2401 attacggtgg tgcaatacct gggaggaaac aatgcaacaa aaaatgattc aatttagtgg
     2461 cgatgtctca ctgccagcca tagggcaggg aacatggtat atgggcgaag atgccagtca
     2521 gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa ctcggtttaa ccctcattga
     2581 taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg gttggggaag cattaaccgg
     2641 tctgcgagag aaggtctttc tcgtctctaa agtctatccg tggaatgctg gcgggcaaaa
     2701 agcgataaat gcatgcgaag ccagtttacg ccgtctcaat actgactatc tcgatctcta
     2761 cttattacac tggtctggca gtttcgcttt tgaagagact gttgctgcga tggaaaaatt
     2821 gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac cttggttatg ccgatatgca
     2881 ggaactctgg cagctgccgg ggggaaatca gtgtgccact aatcaggtgc tttaccatct
     2941 cggttcacga gggattgagt acgatctact cccctggtgc cagcaacagc agatgccggt
     3001 gatggcttac agtccattag cccaggccgg gcggttgcgc aatggactgt taaaaaacgc
     3061 ggtagtcaac gaaattgcac atgtccaaca acatgcagct gcgcttgagg tcgaactttc
     3121 gtcagcggaa ttagctatgc tggataaggc atatccggca ccaaaaggaa aaactgcgct
     3181 ggatatggtg tgatgttgat acgtgccgga tgcatcgcac ccggcacaca acattaacgc
     3241 aaattaacgt ttggcaacgc gaatggattg cgccagatgt gcaggtttct ctttggtgac
     3301 tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca aaggtcttgt agccatcgtc
     3361 cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg ttccagccga caacgttcag
     3421 atgatgctgg tggcctacgg cgataatacg attcagcgct tcatcattaa tcacgctgca
     3481 atcttgcgga ttcagataca cgcgatcggt acggtcaggg aaggtctgaa taccgtcggt
     3541 cagtacatct tctttcgcgt cattcacttt atcaatgaag cgatcgccca gcccactgac
     3601 gcttacctta gcgatatcac ccacgttaaa gtaggtatgc agggcagagg tggtttcaaa
     3661 ttcgccatgt gattcaagat cgatttcaca ggttttaccc acgcggaaat gcgctaacag
     3721 cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt tgtgtcaatt caaaagtcag
     3781 cgctacgcca tcagcatctt catgatgtga tttcagcgtc cacggcaggt tgcgggcaaa
     3841 accgtgcgca ggcagacctt gttgtgccgc cggaccaaac cacggccagc aaaccggtac
     3901 gccaccgcga atagcgacgc catttttgaa cggtgtgttg ttgctcaacc acagaacttc
     3961 ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc tgtaatgcaa aagaggcttt
     4021 tacctgggga tgatcgacca caatgaggtc cagttcatcc agtttacgac gggagaggac
     4081 aggggagatt tgttcgatga ccggaagggc aaaaattttc ttaatcatga cgcagtcctt
     4141 taacttcatt ttatcaggta aaaaaaagag cgaccgaagt cgctcttttt agatcacagt
     4201 gtcatctcaa cttatttgga gatgtgagcg atcaggtcca gaactttgtt ggagtaaccg
     4261 gtttcgttgt cgtaccagga taccagtttc acgaagttgt cgttcagagc gataccagct
     4321 ttagcatcga acacggaagt gcaaacttcg ccgttgaaat cggtagatac tacgtcatct
     4381 tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag cagctttaac ggcagctttg
     4441 atctgctcgt aagttgcagc tttttccaga cgaacggtca ggtcaactac ggatacgttc
     4501 ggggtcggaa cgcggaacgc cataccagtc agtttgccat tcagttctgg cagtacttta
     4561 cctacagctt tagcagcacc ggtagaggac gggatgatgt tctgggaagc gccgcggccg
     4621 ccgcgccagt ctttgtgaga cgggccatca acggttttct gagtagcggt agtagcgtga
     4681 acggtggtca tcagaccttc gatgatgccg aagttatcgt tgataacttt agccagcgga
     4741 gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt cctggccagc atatttgtcg
     4801 aagttagcgc ctttaacgaa catcggagtg ttgtctttag acggaccagt cataaccact
     4861 ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt cagtcaggaa cagaccagtt
     4921 gcttcagcga caacgtcaac acccacttcg tcccatttca ggttagccgg atcacgttca
     4981 gcggtaacac ggattttttt accgttaacg atcagatgac cgtctttcac ttcaacggta
     5041 ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt atgccatgta atcagcgtct
     5101 aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt tctgagcagc acggaaaaca
     5161 atgcgaccga tacggccaaa accgttgata cctactttga tagtcatata ttccaccagc
     5221 tatttgttag tgaataaaag gttgcctgta aaattacaaa aaccttacgc agcgtcaagc
     5281 ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct aagcattacg cgactgactc
     5341 gcctcacttt tcctttgggc ttgagaccac atggggtcgg cgccccgaat tttaaagggc
     5401 aattacgata aaaatgtgat tttcatcacg atttaggtgc agccatttcg tcgagatcaa
     5461 gtttaggaca aaattttgcc agttggtgtt aatgttttgt tagaatcagt cacgtaatgt
     5521 gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa ccttcggcag aagaactgaa
     5581 aaaaaatttg tccgagatgc agttttacgt gacgcagaat catgggacag aaccgccatt
     5641 tacgggtcgt ttactgcata acaagcgtga cggcgtatat cactgtttga tctgcgatgc
     5701 cccgctgttt cattccgaaa ccaagtatga ttccggctgt ggctggccca gtttctacga
     5761 accggtgagt gaagaatcca ttcgttatat caaagacttg tcacatggaa tgcagcgcat
     5821 agaaattcgt tgcggtaact gtgatgccca tctggggcat gtctttcccg acgggccgca
     5881 accaacaggc gaacgttatt gtgttaactc tgcttcttta cgcttcactg atggcgaaaa
     5941 cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact tattccacag gagcggaaaa
     6001 attatgaatc ttgatgacat tatcaacagc atgacgcctg aggtatacca gcgtttgtcg
     6061 accgccgttg aactggggaa atggcctgat ggcgttgcgt tgaccgagga acaaaaagag
     6121 aattgcctgc aactggtgat gctgtggcaa gcccgccata ataccgaagc acagcatatg
     6181 acaattgaca caaatggtca gatggtcatg aagagcaaac agcagttaaa agaagatttt
     6241 ggtatctcag caaagcctat tgccatgttt aagtaagtgg ttcatcccct ttttgccgcg
     6301 gctatgaagg ggatagccaa caatgacccc acgactcgtc tcaattatct cgacccgacc
     6361 tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg cctgaatgac ttctcctgaa
     6421 cttaagtctc agacctattt ggccggtaat ccctctcgaa tttgctcttt ttgcgtcccg
     6481 ccttccacca acctgtaaca gacccagcca ttttctgcgt aattgtgatt tgagttgtaa
     6541 aatgcaggtc attatctgtg ttgtgcagat ttaatattga cctcaaaaat catgtgggta
     6601 agtatgatgg ccatctaagc aaaacaaaac acagaggatg acgcaatgaa agcactggct
     6661 cggtttggca aggcctttgg cggctacaag atgattcatg tcccacaacc catttgtggc
     6721 ccggaagacg tagtgactga aattaaagcc gcggcaatct gcggcgcaga catgaagcac
     6781 tacaatgtcg atagcggttc tgatgagttt aactctatcc gcggccatga gttcgcaggt
     6841 tgtattgcgc aggttggtga aaaagtcaaa gactggaaag tggggcaacg cgtcgtgtcg
     6901 gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg aacagggtga ttttctgtgt
     6961 tgtacagaaa aagtaaacct tggtctggat aacaatacct ggggcggtgg tttttccaaa
     7021 tattgtctgg ttcctggtga aattctcaaa attcatcgtc atgcgttgtg ggaaatccct
     7081 gatggtgttg attatgagga cgcagccgta cttgacccta tctgtaatgc ctataaatcc
     7141 atcgcgcagc aatcgaaatt ccttcctggt caggatgttg tcgtcattgg cactggccca
     7201 ctcgggctgt tttccgtaca aatggcgcgg attatggggg cggtaaatat cgtcgtcgtt
     7261 ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa aagaactggg ggcgacggca
     7321 gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc agcaaatttg tggcaaagat
     7381 aatctgggac tggtgattga atgctccggt gccaatatcg cactgaaaca agccatcgaa
     7441 atgctccgcc cgaatgggga agtggtacgc gttggaatgg gcttcaaacc tcttgatttc
     7501 tcgattaatg acattaccgc ctggaataaa agcatcattg ggcatatggc ctatgactcc
     7561 acctcatggc gtaacgctat ccggctatta gccagcggcg ctatcaaagt caaaccgatg
     7621 atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt ttgatgcgat ggtcgataaa
     7681 accgcaatca aagtgatcat gacttacgac tttgatgaat aactactctc ctgaaggaac
     7741 gttatcgcat ggaacagata acaaaaccgc attgtggtgc acggctggat cgcttaccgg
     7801 attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt tggcttgctt gtctgctgga
     7861 gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc gttgggctgg acagataatt
     7921 ccactacagc cacattctca gcaatcacga ccgccggaat gtttctcggt gcgctggtgg
     7981 gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt catcctctat gaggccattc
     8041 atattgcctc gatggttgtc ggtgctttct caccgaatat ggattttctt attgcctgcc
     8101 gttttgtgat ggacgttggg ctgggagctt tactggttac actgtttgct ggtttcaccg
     8161 aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg ggtttccttt attggcaact
     8221 ggtcatatcc gctctgctca ctgattgcga tgggactcac gccgctgatt agtgcagagt
     8281 ggaactggcg ggtacaactg cttattcctg caatattgtc gcttatcgct acggcgctgg
     8341 cctggcgcta ctttcctgaa tccccgcgct ggctggaatc gcgcggacgg tatcaggaag
     8401 ccgagaaagt catgcggagt atagaagaag gcgtcatacg ccagacgggt aaacctttgc
     8461 cgcctgtggt tattgctgat gacggtaaag cgccacaagc ggtgccgtat tcagccttac
     8521 tgacaggagt attactgaaa cgcgtgatat taggctcttg tgtgctgatt accatgaacg
     8581 ttgtgcagta cacactaatt aactggttgc caacaatatt catgacccag gggattaatt
     8641 taaaagactc gattgtttta aataccatga gtatgtttgg cgcgccattt ggtattttta
     8701 ttgccatgct ggtgatggat aaaattccgc gtaaaacaat gggtgtgggg ctattaatcc
     8761 tgattgcggt gctcggatat atctattcac tgcaaaccag tatgttgctc ataacgctga
     8821 ttggtttctt cctgattact ttcgtctata tgtacgtttg ctatgcctcg gcagtgtatg
     8881 tccctgaaat ctggccgaca gaggccaaac tccgtggctc cggtctggcg aatgcggtag
     8941 ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt gctgctcagt agttatggtg
     9001 taacgggagt ctttattctt ctgggggctg tttcaaatat tgtcgcaatt gctatcgcca
     9061 ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt aagtattgat gcagtagcca
     9121 ataaataata cgggtggatt caaaatgaaa aattcaaaag caatattgca ggtgccgggc
     9181 acaatgaaaa ttatttcagc agaaatacca gtgcctaaag aagatgaagt tttgattaaa
     9241 gtagaatatg tcggtatttg tggttcagat gtacatggtt ttgaatcagg cccgtttatt
     9301 ccgcctaaag acccaaatca agaaattggc ctgggtcatg aatgcgccgg gacggttgtg
     9361 gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc gggtaaatat cgaacctggc
     9421 gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat ataacatctg cccggacgtt
     9481 gattttatgg cgacacaacc caactaccgc ggcgcattaa cgcactatct gtgtcatccg
     9541 gagagcttta cttacaaact gcccgacaat atggacacga tggaaggggc gctggtggag
     9601 cctgccgcag tcgggatgca tgccgcgatg ctggcagatg ttaaaccggg taagaagata
     9661 attattctgg gagcaggttg tattggtttg atgacgttgc aagcgtgcaa atgcctgggc
     9721 gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc tggcaatagg ggaacagcct
     9781 ggtgcgacag tggttattaa tggcgcaaaa gaggacacta ttgcacgctg tcagcaattt
     9841 accgaagaca tgggcgcaga tattgttttc gaaacagcgg gttctgcggt caccgttaaa
     9901 caggcacctt taatctggta atgcgcggcg gtaaaattat gattgttggt actgtacccg
     9961 gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt cactatccag acggtattcc
    10021 gctatgccaa tcgttatccg gtcacgattg aagctatttc ttcagggcga ttcgatgtga
    10081 aatcgatggt gacgcatatt tacgattatc gggatgtaca acaggcattt gaagagtcag
    10141 ttaacaacaa acgcgacatt attaaaggcg ttattaaaat tagcgattaa ttaatacggg
    10201 agaacagaac atgctcgcag atatcagata ttgggaaaac gatgccacaa ataaatatta
    10261 tgcaattgcc cattttaacg tatggaatgc agaaatgttg atgggcgtta aagacgcagc
    10321 cgaagaagcg aaatccccgg ttattatttc ttttagtaca ggttttgtcg gtaacacctc
    10381 atttgaagat ttctctcaca tgatggtatc aatggcacaa aaagcaacgg tgccggtaat
    10441 aactcattgg gatcatggtc ggagtattga gattattcat aacgcctgga ctcatggtat
    10501 gaattcatta atgcgtgatg cttctgcatt tgatttcgaa gaaaatattc gtttaaccaa
    10561 agaggctgtt gatttctttc atccgctggg tattccggta gaggcggaat tagggcatgt
    10621 cggtaatgaa accgtttatg aggaagcgct ggcgggctat cactataccg atcctgacca
    10681 ggcggctgaa tttgttgaac gtacgggctg tgactcattg gctgtcgcca tcggcaacca
    10741 gcatggggtt tatacgtcag agccgcaatt gaactttgag gtcgtcaaac gcgtacgcga
    10801 tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg atcagtgatg ccgacattaa
    10861 aactgcaatt tcgttgggta ttgcgaaaat caacattcat acggagctct gtcaggccgc
    10921 aatggtagca gtgaaagaaa accaggatca gcctttcctg catttagaac gtgaagtacg
    10981 taaagctgta aaagaacggg cattggaaaa aattgaactg ttcggttctg atggcaaagc
    11041 ggaataatga tatggataat ctcgacgtta tttgtatagg tgccgctatt gttgatattc
    11101 cattacaacc ggtcagtaaa aatatctttg atgtagattc ttatcctctt gaaagaatcg
    11161 caatgagcac cgggggcgat gcaataaatg aagcaacaat tatttctcgt ctggggcatc
    11221 gtaccgcatt aatgagccgc attggtaaag atgcctcagg gcaatttatt ctcgaccatt
    11281 gtcgcaaaga gaatattgat attcaaagcc tgaaacagga cgtcagcata gatacatcta
    11341 ttaacgttgg actggtgacg gaggatggcg agcggacgtt tgtcacgaac cgtaacggca
    11401 gtttgtggaa attaaatatt gacgatgttg attttgcgcg gtttcctcag gcgaaattat
    11461 tatcactggc cagtattttc aacagtcctc tattggatgg taaagcgcta acagaaattt
    11521 ttacgcaagc gaaagcccgg cagatgatca tttgtgccga tatgatcaaa ccgcggttga
    11581 atgaaacgct ggatgatatt tgcgaagcat tgagttatgt cgattatctg tttccaaatt
    11641 ttgccgagga aaaattactc acagggaaag agacactgga tgaaattgct gactgctttc
    11701 ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa agacggctgc tttatcaagc
    11761 gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat aaccgccatc gacaccattg
    11821 gcgcgggcga taactttgct tcaggtttta ttgcggcact gttagaaggc aaaaatctgc
    11881 gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc ggttctaagc gtcggtgcca
    11941 ccaccggcgt aaaaaacaga aagctggtcg aacaattgct ggaagaatac gaaggataat
    12001 gaaggcaaat gaaaaagata cctttaggca cgacggatat aacgctttcg cgaatggggt
    12061 tggggacatg ggccattggc ggcggtcctg catggaatgg cgatctcgat cggcaaatat
    12121 gtattgatac gattcttgaa gcccatcgtt gtggcattaa tctgattgat actgcgctag
    12181 gatataactt tggcaatagt gaagttatcg tcggtcaggc gttaaaaaaa ctgccccgtg
    12241 aacaggttgt agtagaaacc aaatgcggca ttgtctggga acgaaaagga agtttattca
    12301 acaaagttgg cgatcggcag ttgtataaaa acctttcccc ggaatctatc cgcgaagagg
    12361 tagaagccag cttgcaacgt ctgggtattg attacatcga tatctatatg acgcactggc
    12421 agtcggtgcc gccatttttt acgccgatag ctgaaactgt cgcagtgctt aatgagttaa
    12481 aggctgaagg gaaaattcgc gctataggcg ctgctaacgt cgatgctgac catatccgcg
    12541 agtatctgca atatggtgaa ctggatatta ttcaggcgaa atacagtatt ctcgaccggg
    12601 caatggaaaa cgaactgctg ccgctatgtc gtgataatgg cattgtggtt caggtttatt
    12661 ccccgctaga gcagggattg ttgaccggca ctatcactcg tgattacgtt ccgggcggcg
    12721 ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct gaaagtgatt gatatgcttg
    12781 aacagtggca gccactttgt gctcgttatc agtgcacaat tcccactctg gcactggcgt
    12841 ggatattaaa acagagtgat ttaatctcca ttcttagtgg ggctactgca ccggaacagg
    12901 tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga tgcagacgca acattgatga
    12961 gggaaatggc agaggccctg gagcgttaaa tactcaacac gattcacctc tgtttctgtg
    13021 aaaactgtga tgaaaatctg tgattcaaat aggttatttt gaaagtaaac atcggttatg
    13081 cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg taatcgtggc ggcaaaagac
    13141 aggattcagg caattaagca aatggttgcc aacgataaaa aggtgacagt ctcaaatttg
    13201 agtgggattt ttcaggtaac cgaagaaacc attcgccgcg atcttgagaa gctggaagat
    13261 gaaggctttt tgaccagaac ctatggtggt gctgttttaa atacagcggt gttgacggag
    13321 aatatccatt tttataagcg cgcttcatcg ttttatgaag agaagctgct tattgcacgc
    13381 aaggcactac cctttatcga caataaaacc accatggcag ccgattccag tagtaccgta
    13441 atggaattgc tcaaattgtt acaggaccgt agtgacctga cgttgctaac caactcggcg
    13501 gaagcgattc atgttctggc tcagtcagaa attaaagtcg tttcaacggg tggggaacta
    13561 aacaaaaata cactttccct gcaaggaaga attactaaag agatcatcag ccgctaccat
    13621 gtcgatatca tggtaatgag ttgtaaaggt cttgatatta acagcggcgc gctggactct
    13681 aatgaagcgg aagctgaaat caaaaagaca atgatccgtc aggcgacaga agttgcgtta
    13741 ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc agttagctga ctttagtcat
    13801 attaattata taataactga taaatcaccg ggtgcagagt ggatagcatt ttgcaaagac
    13861 aataatatcc agctggtgtg gtaatttaag cacacgttgt caatattatt tgttcctgcg
    13921 actggaaata aaatgatgat aaagatgcgg caagtgaatt tgccgcaatt gctacctttt
    13981 atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa ttggcgcaag actggaccgc
    14041 ttgcctttgg cccggtttca ttatcgtata tttggtataa taagctttag tctgttatta
    14101 acggggtttt tgagttactc aggtaatgtc gtcttagcaa agctggtaag caatggatgg
    14161 tcaaataatt tcctctatgc cgcctttacc tcggcattaa tgtttggtta tttcatcggc
    14221 tcacttactg gtgggtttat tggtgactac tttgggcggc gcagggcgtt tcgcataaat
    14281 cttctcatcg tcggtattgc tgcaacaggg gccgcttttg tccctgatat gtactggctc
    14341 attttctttc gcttcctgat gggaacagga atgggggcgc tgattatggt tggctatgcc
    14401 tcatttacgg agtttatccc cgcgacggtg cgtggaaaat ggtccgcgcg gctctcattt
    14461 gttggtaact ggtcgcccat gctgtctgcg gcgataggcg tggtggttat cgcttttttt
    14521 agttggcgaa taatgtttct tctgggtggt attggcatac tgttagcctg gtttctctca
    14581 ggtaaatact ttatcgagtc gccacgatgg ctggaaggga aagggcaaat cgccggtgca
    14641 gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg agaagagtat tcgtttaccc
    14701 cagcttactt tgaaccagag caacagcaag gttaaggtaa tcaagggtac cttcaggctc
    14761 ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga taactgtttt aattgcaatg
    14821 aacatttcgc tttataccat taccgtatgg ataccgacca tatttgttaa ctccggcatt
    14881 gatgtcgata aatcaatatt aatgaccgct gttattatga ttggtgctcc ggtaggaata
    14941 tttattgcgg cattaattat tgatcatttt cctcgtcgat tatttggctc cgccttactt
    15001 attattattg ccgtgttagg ctatatctat tcaagtcaga ctacagagtg ggcgatttta
    15061 atctatggtc tggtgatgat cttcttttta tacatgtatg tttgcttcgc gtcggcggtt
    15121 tatatcccgg agctttggcc aacgcattta cgcctgcgcg gttcgggttt cgttaatgcc
    15181 gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg cggcattatt aacacattat
    15241 gggtcgatca cggtgtttat ggtgcttggt gtcatgttag tgctctgtgc gctggttctc
    15301 tccatttttg gcatcgaaac gcggaaggtg tcgctggaag agatttctga ggtgaattaa
    15361 atctttcgtt gatggcatca attgccagat gtaccactga cgtgttttat ttggcctaca
    15421 atgccagtac agaattgtag gccagcgtaa aattacccct gcgtctcttc ccagtctgcc
    15481 agcgtatata gcgttgcccc agctgctgac atctccataa acgcgtgtgc actgtcctgg
    15541 ggctggatat tcacgccacg acagccatcg gtaatcacgt ttaccttata acctaactgt
    15601 aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag ccaggcccat aacgatcaat
    15661 tcatcgattt catgatcgcg caaccagtca tcaagcgcgg ttttctgccg acgtccgtta
    15721 tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc ctttatggaa caccgctgcg
    15781 atctcttttt gattcagtaa cggatgtaat tgtgcgcctt cactgttctg cacacagtgt
    15841 tcaggccaga aagtttgccg caggccgccg agttggcctg gcgtataagg ctctacgccg
    15901 tgctggctgg caaagctgcc gtgattcgcc ggatgccagt cctggctggc gataaccgct
    15961 tctccgcgcg actggcacca gtcaataagg cggttagcaa cctccaccgt actgtcacct
    16021 tccggtacgg cgagcgcgcc accagcacag aaatcatttt gtaaatcgac cagtaacagg
    16081 gcgcgatggg gcattgcagt ctccttaatc atccggcgtc agttcgccgc gcaggttttg
    16141 gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg ctcagcaggt aatgcagttt
    16201 ggttagcgtg gcttctacag tcatatctgc accgtcaata acgccggcat gggcgagggc
    16261 gttaccggtg gcataaccac tcatattcac tttaccggac atacattgtg tcaggttgac
    16321 caccacaata ccgcgatcgc tggcttcttg taattcctgc aggaaggctt tgttttgtgg
    16381 cgcattaccc acgccatagg agcgcagaat caatgctttc accggttggc gcagaaaatt
    16441 gcgcacgacg tcagcagaaa tccctggata aatcgtcacc acgccaattg gttgtggcgt
    16501 gattggatga acgattaatt ccccttcacc gtgcggggcg ggtggcgtat tcaaacgacg
    16561 aatatggata ccagcttcca gtaacggagg aaggtttgga gaggcaaacg catcaaaacc
    16621 atcggcatgg gctttggtag tgcggttgcc gcgatacaat cggttattga aaaagagcgt
    16681 tacttcgttg atcggataat tcggcgcaac gtacaacgca ttcagcagat taatttgtcc
    16741 gtcagagcgt aactcagcca gcgggatttg tgaccctgtc acaatgaccg gtttaccgag
    16801 attctcgagc atgaacgaca gcgcagaggc ggtatacgcc atcgtgtcgg taccgtgtag
    16861 aatgacaaaa ccatcatagt cgtcatagtg cgctttaata tcttcagcaa tatgctgcca
    16921 gtcttccggc gtcatatcag aagaatccat caacggcgta tattcatgaa tggtgaaatc
    16981 tggcatctcc gggcgatgga attccggcat cagcgccagt tggcgttgta gatgacctga
    17041 caccggaata taaccctgct cggaacgctg catcccgatg gtcccgcccg tgtaggcgac
    17101 gtaaattgat ttcttttgca tgatattgat atgaatgata tcgaaagagc aaaagtatat
    17161 acgtgaggca ggaggagcgc acctgatact atgcggataa ggaaatatgg ggcgagacag
    17221 cgggaagagg attcgtcgag gtaaaaaata atgtggccga cgatctgtcg gccactcagt
    17281 acaagactta acgcacgttg gcgcaggtca ggcaaaacgc ataacggttt tgtgggtcgt
    17341 taaacgcggc cagcttgtcg ctttcacttt taacggtaga ggctaccgag gcaagcggtg
    17401 caggtaacat ggcctggaac gcatctggca acattgcccg gacagaacca gacatgttgt
    17461 ccatcacttt gtcgaagaag gtcggttcat caacgtagta ttccagatgc cactgtttca
    17521 cttttgcgag ctctgctgct ttggcaaccg catcatcgaa atccccgaga ctatcgacca
    17581 gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc ctgggcgatt ttatcaattt
    17641 gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat aaagcgttta tagccattct
    17701 caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag tgccctggtg atagaaacat
    17761 ccgccagcgg tgaagttgag acaccatcag tatgaacacc aatcgaatcc agactatttt
    17821 ctacggtggt gatcacgccg aagataccga tagaaccggt cagggtgctg gggttagcca
    17881 caatgtaatt agctggcgtg gaaatccagt aaccaccaga tgccgccatg ccgcccatcg
    17941 atacaaccac agacttaccc gctgcccggg ctgctgccag ttcagcgcga atcacttcag
    18001 acgcggtaac gctgccgcct gggctattaa cacgcaggac aatcgctttc actttcgggt
    18061 caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc gccaacattc ccctgagttt
    18121 cctcgccatc cataattgcg ccattagcga agacgacacc gatgctgtca ccggtatctg
    18181 ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg ataattttta tcagtcttac
    18241 tccagccgaa ctctttggtc agagcttttt cgatttcggc actcgatgcc agtgcatcga
    18301 ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc ggttttggtt aaaccctcaa
    18361 gcaacccttg cgcgccaggg aatacctgct gagcagggat ctgccggtta gcggcaacag
    18421 tattcagata gttttgccac agctcaccaa tccagcggct gtcagcttcg cgggctgccg
    18481 gtgacatatc atcacgaata aacggttcaa cggcagattt atacgtaccc acgcggaaca
    18541 catgggtgga aactttcagc ttatccagca acgatttgta gtacagaccg ttggtagcaa
    18601 aaccgtgcag atcaaccacg ccttgcggtg acagccaaat tttattggcg aaactggcga
    18661 gataatattg cccctggctg tagttctcgc caacggcata aaccggtttc ccgctgtcac
    18721 gaaactcttt cagggctttg ccgatgtact gcatagacgg ctggtcgccg cctgcgaagt
    18781 ttttcagatc catcacgata ccggtgatat tgcggtcgtc cttcgcctgg cgaatggtgt
    18841 tgacgatatc aaacagtgag ttttcctgca gacgatcgga actggcacca agcagctggc
    18901 ggcttaattt actaaaccgc tgagaactgt ctggtttatc gacgatcaca ccagaaatgt
    18961 ccagcagcag tgcgccacga ctggccgttt ctttcgaatc accaccactg acctgcatcc
    19021 agatccccac accaaccagt acgaggaaaa taaagaacag gttaagtacc atttcacgga
    19081 cgaaattcag cagacgccac gtccatttaa aaaatccggc aataaatcgc caaagggttc
    19141 gcatgtattc tcccaactta aggtcacacc tgtcacaggc gcaatagatg tggttatcgt
    19201 aatgacccaa ccggcacttg tcagcaggaa ttgcggactg tgctgtaaca aaatccactc
    19261 tcgtgttaat tttgtgaata aatatcacga caggagttaa tcaaatggat gcactcgaac
    19321 tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc cgcgccaacg ggtgaacaac
    19381 tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca taagtccatg caaccgtggc
    19441 atttttttgt gattgaaggg gaagggtgcg agcgtttcag cgccgtactg gaacaggggg
    19501 cgattgctgc cggtagtgat gataaagcta tcgacaaagc ccgtaatgcg ccgttccgcg
    19561 caccgctcat catcacggtg gtggcgaaat gcaaagaaaa tcataaagtc ccgcgatggg
    19621 aacaggaaat gtctgccgga tgcgcggtca tggcgatgca aatggcagcc gttgcccagg
    19681 gatttggcgg catctggcgc agtggcgcat taactgaaag tccggtagtg cgtgaagcat
    19741 tcggttgccg tgagcaggat aaaattgtcg gttttctcta cctcggcacg ccgcagttga
    19801 aagcatctac gtcgattaac gtcccggacc cgacgccgtt tgtaacttat ttctgataat
    19861 tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa ttcagacact ctcacttatc
    19921 acttcacgga atgagggtta ccatacccgg attgcattga caggagatgt ccatgagcga
    19981 gaactcgatt cgtttgaccc aatacagcca cggagctggt tgcggctgta aaatttcccc
    20041 aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag tttgttgatc cgaatttgct
    20101 tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat ctgggcaatg gcaccagcgt
    20161 tatcagtacc accgacttct ttatgccgat cgttgataat cctttcgatt ttggccgcat
    20221 tgcggcgact aacgccatca gcgatatctt cgcgatgggg ggcaaaccga ttatggcgat
    20281 tgcgatcctc ggctggccga ttaacaaact ttccccggaa attgcccgcg aagtaaccga
    20341 aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg gctggcggtc actccatcga
    20401 tgcgccggag ccgatttttg gtctggcggt aacggggatc gtaccgaccg agcgggtgaa
    20461 gaaaaacagt accgcacaag ccggatgcaa actgttcctg acgaaaccgc tggggatcgg
    20521 cgttcttacc acggctgaga aaaaatcact gttgaaacca gaacatcagg gactggcgac
    20581 ggaagtgatg tgccggatga acatcgcagg cgcgtccttt gccaacatcg aaggcgtaaa
    20641 agcgatgacc gacgttacgg gctttggtct gctgggccac ttgagcgaaa tgtgtcaggg
    20701 ggctggtgtg caggcacgcg tcgactatga agcgatcccg aaactccccg gtgttgaaga
    20761 gtacattaag ttgggcgcag tacctggcgg cactgaacgt aactttgcca gctacggtca
    20821 tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg tgcgatccgc aaacttctgg
    20881 cggtttgctg ctggcggtca tgccggaagc agaaaatgag gttaaaacta cagccgccga
    20941 gtttggcatt gaactgacgg cgattggcga actggtgcca gcgcgcggcg gtcgtgccat
    21001 ggttgagatc cgttaattta atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg
    21061 ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga tggctttatc gagtgcggta
    21121 atggtcaggt ggtgacttgg tgtatcggtc acctgcttga gcaggcgcag ccagacgcct
    21181 acgacagccg ctatgcgcgc tggaatcttg cggatttgcc gattgtcccg gaaaagtggc
    21241 aattacagcc acgaccctcc gtgaccaaac aacttaacgt catcaaacgc ttcctgcatg
    21301 aagccagcga aatcgttcac gccggggacc cggatcgtga agggcaattg ctggtggatg
    21361 aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca gcaggtgcag cgttgcttga
    21421 taaacgacct gaacccgcag gcggttgagc gggcgatcga ccgtcttcgt tccaacagtg
    21481 agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca
    21541 tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc cggttatcag ggcgtacttt
    21601 ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt gcgccgcgat gaagagattg
    21661 aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca tatcgtgaca cctgccgatg
    21721 agcggtttac cgctatctgg caaccgagcg aagcgtgtga accgtaccag gatgaagaag
    21781 ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa ccgcattagt ggtcaaccgg
    21841 ctattgtcac cagctataac gataaacggg aatcagaatc cgcgccgctg ccgttttcgc
    21901 tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct gagcgcgcag aacgtgcttg
    21961 atatctgcca gaagctgtac gaaacgcaca agctaatcac ttatccgcgt tccgattgtc
    22021 gctatttgcc agaagaacat tttgccggac gccacgcggt gatgaatgcc atcagcgttc
    22081 atacaccgga tctgttgccg cagccagtgg tagatccaga tatacgcaac cgctgttggg
    22141 atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac cgcacggagt tctgcgatca
    22201 acctgacgga gaacgaagcg aaggtctata acctgattgc ccgtcaatac ctgatgcagt
    22261 tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat
    22321 ttgtcgctaa agcccgtttt cttgctgaag caggctggcg cacgctgtta ggcagcaaag
    22381 agcgcgatga agaaaacgac ggcacgccat tgcctgtggt ggcgaaaggc gatgagttgc
    22441 tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc gccgcgccat tttaccgatg
    22501 caacactgct ttcggcgatg accgggatcg cgcgctttgt gcaggacaaa gatctgaaaa
    22561 aggtccttcg tgcgaccgat ggtctgggga cagaagcaac ccgtgccggg attattgaac
    22621 tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta tatccactcc accgacgccg
    22681 gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg accggacatg accgcgcact
    22741 gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc
    22801 cgctggtggg gacgctatat cagcttattg atcaagccaa acgtacgccg gtgcggcagt
    22861 ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga taagaaaaag gctgcaccgc
    22921 gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc cggaagcggg gcgatagcgt
    22981 aagcgagcga atctttcgtg ctattcgagt catattctga aatatccagc ggatcaagaa
    23041 agttcgttgg atattttttt gcatggataa aattatcgcc tctaaagtat gtaataacag
    23101 ggaatgtgat gaaaaaagtt ttattgcaaa accatcctgg gagcgagaag tattctttta
    23161 atggctgggg aatatttaat agtaattttg aacgggtgat taaagaaaat aaggccatgc
    23221 tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc tgtaatgttt gtattcgcag
    23281 cgataacatc caacggtttg aatgaaagag gcctgattac cgcgggatgc tcttttcttt
    23341 atctattaat tatgatgggg cttattgttc gggccggttt taaagcaaaa aaagaacaac
    23401 tgcattatta tcaggctaaa ggtattgagc cgctcagtat cgagaagtta caggcgctac
    23461 aattgatcgc accttatcga ttctatcata agcaatggtc tgaaacgcta gagttctggc
    23521 cgcgaaagcc tgaacctggc aaagatacct tccaatatca tgtgcttcct tttgactcga
    23581 tcgatatcat cagtaaaaga cgcgagtctt tagagaatca atggggtatc gaagatagcg
    23641 aaagttattg tgccttaatg gagcattttc tttctggcga ccatggagcc aataccttta
    23701 aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt gttgaataaa tttgctgttt
    23761 ttccctcaga ctatatctct gattgcgcta ataatcgctc cggtaaatcc tcggcgaagc
    23821 taatatgggc ggcggaatta tcatggatga tctcgatatc aagcacagct tttcaaaacg
    23881 ggacaattga agaagaactg gcctggcatt atataatgct tgcttctcga aaggcgcacg
    23941 agttgttcga aagcgaagaa gattatcaaa aaaatagtct aatgggattt ctttactggc
    24001 atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt tatcgttacg
    24061 acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga aatgttccct
    24121 ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg gcgctgcgct
    24181 tgaccggcct acattagagc gtaactgcaa tgaattgtgc ccatctgtag gcctgataag
    24241 cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc gcatcggcaa
    24301 ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac
    24361 catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt
    24421 tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat ttccaggccc gacgttgcta
    24481 caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc tggaacagtt
    24541 cagtcgcttc gatggtggtc ggcatatttg ccccttcagc gacggcttta acgccattgg
    24601 cgataagctg atgcgcggcg tcaacatcca gttcgttctg ggtggcgcaa ggcagggcga
    24661 tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca aattctttgg
    24721 cgtaatctgc cgctcgacca tcgcggctgg ctttgatttc gataagacgt gccagtttct
    24781 ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca gtgatcacac
    24841 gagcacca
//
LOCUS       DAJVPT010000059        24624 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVPT010000059 DAJVPT010000000
VERSION     DAJVPT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(2..94)
                     /locus_tag="OQM47_002315"
     tRNA            complement(2..94)
                     /locus_tag="OQM47_002315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM47_002316"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM47_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1480443.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM47_002317"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM47_002317"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1480444.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM47_002318"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM47_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1480445.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM47_002319"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM47_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1480446.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM47_002320"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM47_002320"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1480447.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM47_002321"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM47_002321"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1480448.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OQM47_002322"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OQM47_002322"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OQM47_002323"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OQM47_002323"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1480449.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OQM47_002324"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OQM47_002324"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1480450.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OQM47_002325"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OQM47_002325"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1480451.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OQM47_002326"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OQM47_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS1480452.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OQM47_002327"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OQM47_002327"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1480453.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM47_002328"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM47_002328"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1480454.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM47_002329"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM47_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1480455.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OQM47_002330"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OQM47_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1480456.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OQM47_002331"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OQM47_002331"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1480457.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM47_002332"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM47_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1480458.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM47_002333"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM47_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1480459.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM47_002334"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM47_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1480460.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OQM47_002335"
     CDS             complement(20900..21019)
                     /locus_tag="OQM47_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCS1480461.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM47_002336"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM47_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1480462.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM47_002337"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM47_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1480463.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24624)
                     /locus_tag="OQM47_002338"
     CDS             complement(23682..>24624)
                     /locus_tag="OQM47_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274453.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1480464.1"
                     /translation="GLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVL
                     FLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQS
                     FFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAP
                     LLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLL
                     PFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLN
                     PVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5805 a         6941 c         6313 g         5565 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc accactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag cccc
//
LOCUS       DAJVPT010000060        23914 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVPT010000060 DAJVPT010000000
VERSION     DAJVPT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..376)
                     /locus_tag="OQM47_002339"
                     /pseudo
     CDS             complement(1..376)
                     /locus_tag="OQM47_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            900..2828
                     /gene="thrS"
                     /locus_tag="OQM47_002340"
     CDS             900..2828
                     /gene="thrS"
                     /locus_tag="OQM47_002340"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1480465.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2832..3374
                     /gene="infC"
                     /locus_tag="OQM47_002341"
     CDS             2832..3374
                     /gene="infC"
                     /locus_tag="OQM47_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1480466.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3471..3668
                     /gene="rpmI"
                     /locus_tag="OQM47_002342"
     CDS             3471..3668
                     /gene="rpmI"
                     /locus_tag="OQM47_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1480467.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3721..4077
                     /gene="rplT"
                     /locus_tag="OQM47_002343"
     CDS             3721..4077
                     /gene="rplT"
                     /locus_tag="OQM47_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1480468.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4194..4318
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4200..4244
                     /gene="pheM"
                     /locus_tag="OQM47_002344"
     CDS             4200..4244
                     /gene="pheM"
                     /locus_tag="OQM47_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1480469.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4527..5510
                     /gene="pheS"
                     /locus_tag="OQM47_002345"
     CDS             4527..5510
                     /gene="pheS"
                     /locus_tag="OQM47_002345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1480470.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5525..7912
                     /gene="pheT"
                     /locus_tag="OQM47_002346"
     CDS             5525..7912
                     /gene="pheT"
                     /locus_tag="OQM47_002346"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1480471.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7917..8216
                     /gene="ihfA"
                     /locus_tag="OQM47_002347"
     CDS             7917..8216
                     /gene="ihfA"
                     /locus_tag="OQM47_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1480472.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8317..9297
                     /gene="btuC"
                     /locus_tag="OQM47_002348"
     CDS             8317..9297
                     /gene="btuC"
                     /locus_tag="OQM47_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1480473.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9360..9911
                     /gene="btuE"
                     /locus_tag="OQM47_002349"
     CDS             9360..9911
                     /gene="btuE"
                     /locus_tag="OQM47_002349"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1480474.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9911..10660
                     /gene="btuD"
                     /locus_tag="OQM47_002350"
     CDS             9911..10660
                     /gene="btuD"
                     /locus_tag="OQM47_002350"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1480475.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10738..11202
                     /locus_tag="OQM47_002351"
     CDS             10738..11202
                     /locus_tag="OQM47_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1480476.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11449..12162
                     /gene="ydiV"
                     /locus_tag="OQM47_002352"
     CDS             11449..12162
                     /gene="ydiV"
                     /locus_tag="OQM47_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1480477.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12226..13662
                     /locus_tag="OQM47_002353"
     CDS             12226..13662
                     /locus_tag="OQM47_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1480478.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OQM47_002354"
     CDS             complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OQM47_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1480479.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OQM47_002355"
     CDS             complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OQM47_002355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1480480.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OQM47_002356"
     CDS             complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OQM47_002356"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1480481.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="OQM47_002357"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="OQM47_002357"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1480482.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OQM47_002358"
     CDS             complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OQM47_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1480483.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20495..20788)
                     /locus_tag="OQM47_002359"
     CDS             complement(20495..20788)
                     /locus_tag="OQM47_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1480484.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="OQM47_002360"
     CDS             complement(20785..22074)
                     /locus_tag="OQM47_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1480485.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="OQM47_002361"
     CDS             complement(22130..23068)
                     /locus_tag="OQM47_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1480486.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="OQM47_002362"
     CDS             complement(23088..23852)
                     /locus_tag="OQM47_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1480487.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5907 a         5799 c         6297 g         5911 t
ORIGIN      
        1 tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg cgtggtttct
       61 agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg
      121 atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt
      181 ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa cttaagtcag
      241 aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct gatgaaggtg
      301 ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga
      361 tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg aggatgatga
      421 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt
      481 ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc
      541 aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa
      601 acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct ggtgtactca
      661 ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct cccttttttt
      721 agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca tttgtgtacc
      781 tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac tgttaataaa
      841 caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa ggatataaaa
      901 tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct gtaagcccca
      961 tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta
     1021 atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg tcgatcatta
     1081 ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc
     1141 atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt attgacaacg
     1201 gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc gaagcactcg
     1261 agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag aaagtcagct
     1321 ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt tccattcttg
     1381 acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa tatgtcgata
     1441 tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa
     1501 cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt atttacggta
     1561 cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa gaagccgcga
     1621 aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc
     1681 cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg gaagtgtttg
     1741 ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc atgatggacc
     1801 gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg ttcaccacat
     1861 cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac gtacaaattt
     1921 tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag tttggtagct
     1981 gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc
     2041 aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt aacggatgta
     2101 tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc gtcaaactct
     2161 ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct gaggcggacc
     2221 tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt gaaggcgctt
     2281 tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca tggcagtgcg
     2341 gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag
     2401 acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg atggagcgtt
     2461 tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt gcgccagttc
     2521 aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa ttgacgcaaa
     2581 aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag attggcttta
     2641 aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt gataaagagg
     2701 tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga agcatggacg
     2761 taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt aaacaattgg
     2821 aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg
     2881 gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag cttggtattg
     2941 tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta gtcgagatca
     3001 gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc ctctatgaaa
     3061 agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta
     3121 aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc ctgattcgct
     3181 ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc
     3241 accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag
     3301 tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg ctcgctccta
     3361 agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc ttcacaggca
     3421 ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa atgccaaaaa
     3481 ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc
     3541 acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc
     3601 tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt
     3661 acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag gagagcacat
     3721 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     3781 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     3841 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     3901 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     3961 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     4021 ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct ggcataagcc
     4081 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     4141 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     4201 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag gaggctagcg
     4261 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     4321 gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac
     4381 acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga gcactgcccg
     4441 ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa cgaaccagtg
     4501 tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt gccagtgcga
     4561 aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt
     4621 tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg ccgccagaag
     4681 agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag caggcgctga
     4741 atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga
     4801 ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg gttacccgta
     4861 ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca accgggccgg
     4921 aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac cacccggcgc
     4981 gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc cagacctctg
     5041 gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc gcgcctggcc
     5101 gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag atggaaggtc
     5161 tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac gacttcctgc
     5221 gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg
     5281 agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa gtgctgggct
     5341 gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa gtttactctg
     5401 gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc gtcaccgacc
     5461 tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata
     5521 gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga tcgatagcga
     5581 tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag aaccggttgc
     5641 cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc atccgaacgc
     5701 tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg
     5761 cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg gtgctgttct
     5821 gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg aagggatgct
     5881 gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg aactgcctgc
     5941 ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata acaccatcga
     6001 aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg cgcgtgacgt
     6061 tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg gtgcgactat
     6121 cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct atcttggccg
     6181 tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg
     6241 tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg tactgctcga
     6301 actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg
     6361 gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga agctgaatgc
     6421 tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca tctttggtgg
     6481 cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag
     6541 cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg cgtctcaccg
     6601 ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg cgacccgtct
     6661 gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca acgaagcaac
     6721 gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc tgataggcca
     6781 tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg aagtgaccga
     6841 aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg agattgaaga
     6901 agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg atgagccggt
     6961 acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa
     7021 aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg ttgatccgaa
     7081 agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc cgatctctgt
     7141 tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa
     7201 ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga
     7261 tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa
     7321 ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg atttgaaagg
     7381 cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt tccgtgcaga
     7441 agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat
     7501 tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta acggtcgcac
     7561 tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga
     7621 gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc
     7681 cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa
     7741 cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc tcgccataag
     7801 cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg ctaccgtcgc
     7861 caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt gaacctatgg
     7921 cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt agcaagcggg
     7981 atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg
     8041 aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat caacgcccgg
     8101 gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg gtgaccttca
     8161 gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac gagtaatctg
     8221 atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag agtcaccgta
     8281 aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa
     8341 cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc
     8401 ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac tcctcgtggc
     8461 gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg
     8521 gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct
     8581 ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt attgcttggg
     8641 caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc
     8701 actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct
     8761 ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta cttttccacc
     8821 tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg
     8881 cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc
     8941 aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg tttacccatg
     9001 tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt cagtgtggcg
     9061 ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg gttgtgtggt
     9121 ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg
     9181 ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc
     9241 accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg acgttagccg
     9301 caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta ggagaaacga
     9361 tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg
     9421 agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc
     9481 cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc
     9541 tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa gagattaaaa
     9601 cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg
     9661 gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc acagtcgcgc
     9721 cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg ctgtacccgg
     9781 atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa gtcatccagc
     9841 gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt aaactggcgt
     9901 tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta cccgcctggg
     9961 gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc cgaatggcgc
    10021 gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg gaagcattca
    10081 gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta
    10141 tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca
    10201 tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga
    10261 tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct
    10321 tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat tgctgcttct
    10381 tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag
    10441 cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacgtt
    10501 gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca gtggacgcag
    10561 agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct
    10621 ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt atcccgaaaa
    10681 tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg
    10741 cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca
    10801 ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag
    10861 ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac
    10921 tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa
    10981 accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct gcctggtgac
    11041 ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat ttacgatacc
    11101 aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat
    11161 gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga aaaattgacg
    11221 gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca gacaaaaatt
    11281 ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt
    11341 ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt atggcctcat
    11401 cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat gaagattttt
    11461 ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa ccagcaactc
    11521 cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt tcgtatcccg
    11581 acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt ttcagagcag
    11641 ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt tgtctggctt
    11701 aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc aggcgaacta
    11761 ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct caatgaagga
    11821 aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt aggcaatttg
    11881 ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg
    11941 gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt tattcgtgcg
    12001 atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat tgatacgcca
    12061 gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg cctgtggcct
    12121 gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg tttgctggtg
    12181 tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac cctgtctttt
    12241 attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc tacgccttta
    12301 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
    12361 tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
    12421 ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca actgggtgat
    12481 gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    12541 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    12601 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    12661 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt ggagccaggc
    12721 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    12781 tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg tcattactgg
    12841 tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt tgtcgcacgt
    12901 accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt gatgaatacc
    12961 gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt tcttgatgat
    13021 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat
    13081 caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt
    13141 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    13201 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    13261 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    13321 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga gtttattgat
    13381 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
    13441 agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt attgcgcaac
    13501 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga attgcaccgc
    13561 ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt cagtcgtcca
    13621 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tagttaacag
    13681 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    13741 tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct gcggggaagc
    13801 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
    13861 aattaataat aattgatata acaatgattc ctattatcaa tattatggca gaccagagca
    13921 agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
    13981 gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag gcgttcggta
    14041 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
    14101 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatctccgta
    14161 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
    14221 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
    14281 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
    14341 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
    14401 tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
    14461 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga agccatttcg
    14521 cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat taaatcagca
    14581 ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
    14641 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
    14701 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
    14761 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
    14821 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
    14881 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
    14941 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
    15001 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
    15061 ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca gctgatttca
    15121 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa acaaaccaat
    15181 gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat cttggtggca
    15241 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaag
    15301 gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg actgttctcc
    15361 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
    15421 tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc ggcaataaag
    15481 gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt gggggtttta
    15541 ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg caacgaaatg
    15601 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
    15661 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
    15721 tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc actttgcaag
    15781 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacgcctgtc
    15841 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
    15901 aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
    15961 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
    16021 tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat taaaagcgca
    16081 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat tttttataaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccgccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaat
//
LOCUS       DAJVPT010000061        23866 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVPT010000061 DAJVPT010000000
VERSION     DAJVPT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(25..597)
                     /locus_tag="OQM47_002363"
     CDS             complement(25..597)
                     /locus_tag="OQM47_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1480488.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OQM47_002364"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OQM47_002364"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1480489.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OQM47_002365"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OQM47_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1480490.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMSSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OQM47_002366"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OQM47_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1480491.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OQM47_002367"
     CDS             complement(3559..5340)
                     /locus_tag="OQM47_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1480492.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OQM47_002368"
     CDS             complement(5333..7105)
                     /locus_tag="OQM47_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1480493.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OQM47_002369"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OQM47_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1480494.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OQM47_002370"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OQM47_002370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1480495.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="OQM47_002371"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="OQM47_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="OQM47_002372"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="OQM47_002372"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1480496.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OQM47_002373"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OQM47_002373"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1480497.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="OQM47_002374"
     CDS             complement(11661..12032)
                     /locus_tag="OQM47_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1480498.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OQM47_002375"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OQM47_002375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1480499.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OQM47_002376"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OQM47_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1480500.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OQM47_002377"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OQM47_002377"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1480501.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OQM47_002378"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OQM47_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1480502.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OQM47_002379"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OQM47_002379"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1480503.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OQM47_002380"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OQM47_002380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1480504.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OQM47_002381"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OQM47_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1480505.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="OQM47_002382"
     CDS             21803..22381
                     /locus_tag="OQM47_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1480506.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..>23866
                     /gene="ampG"
                     /locus_tag="OQM47_002383"
     CDS             22425..>23866
                     /gene="ampG"
                     /locus_tag="OQM47_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1480507.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLL"
BASE COUNT         5581 a         6601 c         5784 g         5900 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgctca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctgga
//
LOCUS       DAJVPT010000062        23506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVPT010000062 DAJVPT010000000
VERSION     DAJVPT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..600)
                     /gene="rluF"
                     /locus_tag="OQM47_002384"
     CDS             complement(<1..600)
                     /gene="rluF"
                     /locus_tag="OQM47_002384"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1480508.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEV"
     gene            948..1895
                     /locus_tag="OQM47_002385"
     CDS             948..1895
                     /locus_tag="OQM47_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1480509.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            1965..2771
                     /locus_tag="OQM47_002386"
     CDS             1965..2771
                     /locus_tag="OQM47_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1480510.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            2781..3188
                     /locus_tag="OQM47_002387"
     CDS             2781..3188
                     /locus_tag="OQM47_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1480511.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3188..3682
                     /locus_tag="OQM47_002388"
     CDS             3188..3682
                     /locus_tag="OQM47_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1480512.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            3748..4545
                     /locus_tag="OQM47_002389"
     CDS             3748..4545
                     /locus_tag="OQM47_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1480513.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4556..5344
                     /locus_tag="OQM47_002390"
     CDS             4556..5344
                     /locus_tag="OQM47_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1480514.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5396..6625
                     /gene="sorE"
                     /locus_tag="OQM47_002391"
     CDS             5396..6625
                     /gene="sorE"
                     /locus_tag="OQM47_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1480515.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7007..7696
                     /gene="pepE"
                     /locus_tag="OQM47_002392"
     CDS             7007..7696
                     /gene="pepE"
                     /locus_tag="OQM47_002392"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1480516.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7787..9418)
                     /locus_tag="OQM47_002393"
     CDS             complement(7787..9418)
                     /locus_tag="OQM47_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1480517.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9638..13321)
                     /gene="metH"
                     /locus_tag="OQM47_002394"
     CDS             complement(9638..13321)
                     /gene="metH"
                     /locus_tag="OQM47_002394"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1480518.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13521..14345
                     /gene="iclR"
                     /locus_tag="OQM47_002395"
     CDS             13521..14345
                     /gene="iclR"
                     /locus_tag="OQM47_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1480519.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14662..16846
                     /gene="espL4"
                     /locus_tag="OQM47_002396"
                     /pseudo
     CDS             14662..16846
                     /gene="espL4"
                     /locus_tag="OQM47_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(16815..18552)
                     /gene="aceK"
                     /locus_tag="OQM47_002397"
                     /pseudo
     CDS             complement(16815..18552)
                     /gene="aceK"
                     /locus_tag="OQM47_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(18736..20040)
                     /gene="aceA"
                     /locus_tag="OQM47_002398"
     CDS             complement(18736..20040)
                     /gene="aceA"
                     /locus_tag="OQM47_002398"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCS1480520.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20070..21671)
                     /gene="aceB"
                     /locus_tag="OQM47_002399"
                     /pseudo
     CDS             complement(20070..21671)
                     /gene="aceB"
                     /locus_tag="OQM47_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(21940..22869)
                     /gene="metA"
                     /locus_tag="OQM47_002400"
     CDS             complement(21940..22869)
                     /gene="metA"
                     /locus_tag="OQM47_002400"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1480521.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23026..23469
                     /locus_tag="OQM47_002401"
     CDS             23026..23469
                     /locus_tag="OQM47_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1480522.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5806 a         5991 c         5968 g         5741 t
ORIGIN      
        1 cacttcatag ccgaaatgct cgcacatgcg ccggatctga cggttcagcc cctgcactag
       61 ggtaatgcgg aagacaaacg gcgcttcttt tttgactttg cactttttcg tcaccgtccc
      121 gaggatcggc acccccgccc ccataccacg aataaattca tcggtaatcg gtttatcgac
      181 cgtcaccaga tactctttct catgatcgtt acccgcacgg aggatcttat tcaccagatc
      241 gccgtgattg gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc caatcgggaa
      301 cacgcgtttg ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat cttcggtggt
      361 gctgacaata ccgacgggct tgttcagggc gataagtacc aaatcttcgg cttcccgagg
      421 ctcaatcaac tgaccgttta ctttcacaat gtcgccgggt ttcacctgat cgccaatggt
      481 ggctcgcttg ccattaagga acacattgcc ttgctcgata tagcgatccg cttcgcggcg
      541 tgagcaaatt ccgctttcgc tgatgtattt atttaaacgg actgatgagt cgggcagcat
      601 aaattctcct gtaaaacgcg gaatataccg tacctcttag cggggaaaaa cagggttatg
      661 cggcaacgtc gaaattaaat gtgatctgcc acgcctttta tcacaaaagt gtctccggaa
      721 gtacgatgca aatttgcctg gcaaaataag gataatcaac atattacgaa tcgctgcaca
      781 cctgtgctga tgactactac gcagagaaac aaatgtgcaa gagaaagcgc tatttctccc
      841 catgcaaaga gtgaaaaatc gctttgaaag gccctcatga tggggttata tcagatcaag
      901 cggtgagaaa gcgtatttgc acaaatgtgc agaaccagga ggcggcaatg gaaaacagtg
      961 acgatatccg tttgattgtg aagattgccc aactctatta cgaacaggat atgacgcagg
     1021 cgcaaatcgc gcgcgaactg ggtatttacc gcaccaccat cagccgcttg cttaaacgag
     1081 gccgcgatca gggaatggtc accatcgcca tcaactatga ctacaacgaa aatctctggc
     1141 tggagcagca actgaagcaa aagtttggcc tgaaagacgt tgtggtggtg tcgggaaatg
     1201 atgaggatga agagactcaa ctggcgatga tggggttaca cggcgcgcaa ctgctggatc
     1261 gcttgctgga gcctggcgat attgtcggtt tttcctgggg tcgcgcggtg agcgcactgg
     1321 ttgaaaactt gccgcaggcg gggcaatcgc ggcagttaat ctgcgtacca attattggcg
     1381 gaccgtccgg taaactcgaa agccgctatc atgtaaacac attaacctac agcgcggcag
     1441 cgaagctgaa aggggaatcg catctcgcgg attttccggc tctgctggat aacccattaa
     1501 ttcgtaatgg gatcatgcag tctcagcact ttaaaaccat ctctgcctac tgggataatc
     1561 tggatatcgc cctggtggga attggctcac cggccattcg cgacggcgct aactggcatg
     1621 cgttttatgg tggtgaagag agtgacgacc tgaatgcccg ccaggttgct ggcgatattt
     1681 gctcgcgctt ttttgatatt cacggcgcaa tggttgaaac gaatatgagc gaaaaaacgc
     1741 tctctatcga aatgaataaa ttaaagcagg cgcgatattc cattggcatt gccatgagtg
     1801 aagaaaaata cagcggaatt attggtgcac tgcgtggaaa atatattaat tgtctggtaa
     1861 cgaatagcag cacagctgaa ctattactga aataaaaatc ttcccgatgt ttaccgtaat
     1921 aaaaattacg gcgggtccct attatctaaa atcaggagtc tgttatgcaa acgtggttaa
     1981 atttgcagga taaaatcatt attgtcaccg gcggcgcatc cggtattggt ctggcgattg
     2041 tagaggaatt attagcacaa ggcgcgaatg tacagatggt cgatattcac ggtggcgatg
     2101 gtcaatatga aggtcataaa ggctatcagt tttggccgac cgatatttct agcacaaaag
     2161 aggtaaatca tacggtagcg gaaattatcc agcgttttgg tcgcatcgac ggtctggtca
     2221 ataacgccgg ggtcaatttc ccgcgtctgc tggtcgatga gaaagcgcct gccgggcagt
     2281 atgaactcaa cgaagctgca ttcgaaaaaa tggtcaatat caaccagaaa ggtgtttttc
     2341 tgatgtcgca ggcggtggcg cgacagatgg tcaaacaaca tgatggcgtg attgtgaatg
     2401 tttcctcaga aagtgggctg gaaggctcag aaggacaaag ctgttacgcc gctaccaaag
     2461 ccgcgctcaa tagcttcacg cgctcctggt cgaaagagct gggtaaacac ggtatccgtg
     2521 tggtcggtat cgcgcccggg attctggaaa aaaccgggct acgcacgccg gaatatgaag
     2581 aggcgctggc gtggacgcgc aatatcaccg tcgagcagct gcgtgaaggc tataccaaaa
     2641 acgccattcc tattgggcgc gccggaagat tagcagaagt agctgatttt gtttgttatc
     2701 tgttgtctga acgcgccagc tacatcaccg gagtaaccac taacattgcg ggcggcaaaa
     2761 cgcgcggcta aggaggcttt atggtcaacg ccattttttg tgcccacggc aaattagctt
     2821 gcgccatgct ggaatcggtt cagatggttt acggcaacgc caatgttgaa gccgttgcat
     2881 ttgtgcccgg tgaaaacgcc ggagacattg cggtaaagct ggaaaagtta gtaagcattc
     2941 acaatcaaga tgagtggctg attgccgtgg atttacagtg cggcagcccg tggaatgcgg
     3001 cagcaatgct tgcgatgcgc aatcctcgtt tgcgggtaat cagcggcctt tctctgccgc
     3061 tggcgctcga actggttgat aaccaggaca gcatgaatgt tgatgaactg tgtgaacacc
     3121 tgacgcaaat cgcaaagcaa acctgcgttg tctggaaaca actggcaacg acagaggagg
     3181 atttctgatg aacattacgc tcgcccgcat tgatgaccgt ctgatccacg gccaggtcac
     3241 taccgtctgg tcgaaagtgg ctaacgccca gcgcattatt atctgcaatg acgaagttta
     3301 taacgatgaa gtgcgccgga cattattgcg tcaggctgcg ccaccgggaa tgaaggttaa
     3361 cgtggtcaat attgaaaaag ccgtcgcggt ttatcacaac ccgcaatatc aggacgaaac
     3421 cgttttttat cttttcactc gcccacagga tgccttagcg atggttcgcc agggggtaaa
     3481 aataggcacc ctgaatattg gcggcatggc ctggcggccc ggtaaaaaac agttaaccaa
     3541 agcggtttca ttagatgatg atgatattaa cgcatttcat gagttaaata atcttggtgt
     3601 catactggat ttacgcgtcg tagcgtcaga tccctcaatc aatattatcg acaaaataaa
     3661 cgaacagtta atcgcaaatt aaataatagc gcctgtgtac atatgcttta acaggcaggg
     3721 atgacgacac ttaaaaggtg acaaatcatg gaaataagta ccctacaaat cattgctata
     3781 tttctttttt cctgtattgc cggaatgggc agcgtgctgg atgaatttca gactcatcgc
     3841 ccgttaattg cctgtacggt gattggttta attctcggtg atttaaaaac cggaataata
     3901 ctcggcggta cgctggaatt gatagctctc tgctggatga acgtcggcgc ggcgcaatct
     3961 ccggattctg cactcgccag cataatctcc gccattctgg ttatcgttgg tcagcaaagc
     4021 atcgccaccg gaatcgccat cgcgttgcct gtggctgcgg caggccaggt gctgacggtg
     4081 tttgcccgta ccatcaccgt agtgttccag cacgcggcgg ataaagcagc agaagaggcg
     4141 cggtttcgca ctctcgatat tctgcatgtc tccgcgcttg gcgtgcaggc gctgcgcgtc
     4201 gccattccgg cactgattgt ctcactgttc gtcagcgccg atatggtcag caatatgtta
     4261 aacgccattc ccgaatttgt gacccgagga ctacagattg ctggcggttt tatcgtggtg
     4321 gtcggttacg ccatggtgct acgtatgatg ggcgtgaaat atttgatgcc tttctttttc
     4381 ctcggtttcc tcgcaggtgg ctacctcgat ctcagcctgc tggcgttcgg tggcgtcggc
     4441 gtgatcatgg ccctggtcta catccagcta aatccacagt ggtgtaaagc tgaacctcaa
     4501 ctccaggcca ccgcctccac cgcccttgac caacttgacg attaacggag ccatcatgga
     4561 acagagaaaa attacacgca gcgatctggt gagcatgttt ctgcgctcca acctgcaaca
     4621 ggcgtccttt aactttgaac gtattcacgg gctgggcttt tgctacgaca tgatccccgc
     4681 catcaagcga ctttacccat taaaagagga tcaggttgcg gcgctcaggc tacacctggt
     4741 gttcttcaat accacgccag ccgtatgtgg cccggtcatc ggcgcggaaa ttgatgacgg
     4801 taccatcaac ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact
     4861 ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc gcatctctgg cactttcggg
     4921 caacattctc ggcccgctgc tgttcttctt tattttcaac gcggtgcgtc tggcgatgaa
     4981 gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg
     5041 gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt
     5101 gctggtgacc aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcatgccgc
     5161 cgatggctcc accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt
     5221 gctggcgctc ggtctgacgc tactaatggt tcgtctgctc aacaaaaaaa ttaacccggt
     5281 atggctgatt ttcgccctgt ttggcttagg gattatcggc aatgctctgg gcttcctgtc
     5341 ctgattcttc gccccggcac gactgccgag gccatcgctc aacatgaggt ggtttatgaa
     5401 aacaacagct ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct ttgaccttcc
     5461 tgaaatgcag gaggatgaaa tcctcgcaac ggtggtcact gacagcctgt gcctctcttc
     5521 ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tgacgaccaa
     5581 cccaatcatc atcggccacg aattttgcgg cgatattctg gccgtgggta aaaagtggca
     5641 gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg
     5701 cccggattgc cccggctact ccttcccgtg ggtagacggc gaagcgacgc atgtggttat
     5761 tcccaacgag gtcatggaac aatattgcct gctggcatac gacggcgaaa cctattttga
     5821 aggctcactg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct
     5881 tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcaca cgctgatcct
     5941 cggcggcacc ggaccaatgg gactgttggc gattgattat gcgctgcatg gaccagttaa
     6001 cccgtcgctg ctcgtcatta ccgataccga caacgacaaa ttgagttatg cgcgcaagca
     6061 ctatccatca gaaccgcaaa cactgattca ttatctcaat gccaccgatg cagcatttga
     6121 tacgttaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa
     6181 tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt
     6241 cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc
     6301 atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa
     6361 actgatcgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa
     6421 tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta
     6481 taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc
     6541 gattctggcg cgtcatcagg ggatctggtc cggtgaggtg gagcaatacc tgctcgccca
     6601 tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc
     6661 gccccagcaa cttcgcgacc ggatgtgcgg ctgattgctg aagcagcaca atttgcacga
     6721 gaaggataaa aaatatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
     6781 tttatgccgg atgcggaatg aagccttatt cggtctacat aattttgaaa atccaatata
     6841 ttgcagttat ctggtaggcc aggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
     6901 agtctcagcg cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
     6961 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
     7021 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
     7081 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
     7141 ctgggatgat tacacagaga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
     7201 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgcggaaa ttgtgattgt
     7261 cggcggcggt aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggctcc
     7321 aattactgac gtggttaaac gtggcgcgct gtatattggc tggagcgcag gcgctaacct
     7381 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
     7441 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
     7501 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
     7561 gacgattatt ggtctgccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct
     7621 gggtggcccg aacaccactt atgtgtttaa ggctggtgaa gaagcggttc cgctggaagc
     7681 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
     7741 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
     7801 gtccttcatc ttcatccggc tgttccagca cactgtaagc caccgaacaa aacagcgagt
     7861 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
     7921 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
     7981 aacgatgttt gctgcgacgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
     8041 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
     8101 catcaagcga aaatgcccgc cgtgctgcca gcgatttatc ggcaatttcg ctgcccatgc
     8161 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc
     8221 ggcgcgactc ttcctccgcc aactcttctt ttggcatccg cgccagatac agcttaatgg
     8281 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
     8341 cgccgtgcat cactttattc agcccttcca tcatctgttc catggcgtcg ccaatgcgca
     8401 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt gtccagcgcg ctgacatcca
     8461 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
     8521 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
     8581 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
     8641 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
     8701 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
     8761 aaccgaggtt agctccaatc accagacaga gcgccacggg gaaggagata atgcctgcag
     8821 cggtcagagt cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
     8881 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
     8941 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
     9001 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
     9061 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
     9121 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
     9181 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
     9241 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
     9301 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
     9361 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
     9421 gttcagattc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
     9481 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
     9541 ccagcctgtt tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
     9601 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
     9661 tcggtgccag ccagcgctca acatcggaga cgctcatacc tttacggcgg gcatggtctt
     9721 caacctgatc gcgctgaatt tgcgccaccg cgtagtactt gctgtccgga tgactgaaat
     9781 accaacccga aaccgatgca ccaggccaca tcgcgaaaga ttctgtgagt ttcatgccag
     9841 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
     9901 ccggatagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
     9961 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    10021 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    10081 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    10141 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    10201 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgacg ttgatcacat
    10261 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    10321 ggcccaccac gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt
    10381 tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg
    10441 gatacttccc ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca
    10501 gcgtttcgat gctggcttcc acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt
    10561 aagcctgcca gtcaaatgcg aaatcgttat cgcgcgccgc ttccagcgtg accggtggtg
    10621 tgcgtggttt cttgcgcccg tgctgaatac gcacggtttc gtactccttg caggtacgag
    10681 cgacaaaatc atcacgctga gtatcggaaa gcagcgccgc taccacacca acggtgcgcg
    10741 aggcattctg cacatacacc gtagggccgc tgtagttctg ctcgattttc accgccgtgt
    10801 gcgcttttga ggtggtcgcg ccgccaatca gtaacggaat ggcgaagccc tgacgctcca
    10861 tctctttcgc cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa
    10921 tcaaatcagc attcacttct ttggcgatac ggagaatttt ttccgcaggc accataacgc
    10981 cgagatcgac aatttcgtag ttgttacatt gcagcaccac accaacgata tttttaccga
    11041 tgtcgtggac atcgcctttc acggtggcga tcaccatctt gccgttggtt ttaccctgct
    11101 ctttgctggc ttcaataaac ggttcgagat acgccactgc ctgtttcatg acgcgcgccg
    11161 atttgaccac ctgtggcagg aacattttgc cttcgccaaa cagatcgccg accacgttca
    11221 tgccgtccat caacgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt
    11281 cttcagtatc ctgctcgata aactcggtta tgcctttgac cagcgagtat tccagacgtt
    11341 tattcacgtc ccacgagcgc cactccgctt gctgggcgtt agcagtgtcg tccgctttac
    11401 tgccgcgata tttctcggca agctccagta aacgctcagt gccatcgtca cgacgattaa
    11461 gaatcacatc ttccaccgcg tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt
    11521 gcccggcgtt gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacactg
    11581 cgtgaatggc ttcgcgcacc ggatcgttgc cacggaatga gaaagaaacg ttagatacgc
    11641 cgccggaaat cagcgcgtgc ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt
    11701 cctgcgcgta gttgttgtgc tcttcaatac cggtggcgac cgcgaagatg ttcgggtcga
    11761 agatgatatc ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc
    11821 aaatctcgat tttccgtgcg cgagtatcgg cctgtccctg ttcgtcaaag gccatcacca
    11881 ccactgccgc accgtagcga cgcaacaatt tcgcgtgatg gataaaggcg tcgacgccct
    11941 ctttcatcga gatagagtta acaatgcctt tgccctggat acacttcaga cctttttcaa
    12001 tgacatccca ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg
    12061 caatcagatt gagaaaacgc accatcgccg cttcggcatc gagcatccct tcatccatgt
    12121 tgatatcgat aatctgcgcg ccgttttcca cctgctgacg cgccacatcc agcgcctcgc
    12181 tgtatttctc ttctttgatc aggcgcttga acttagcaga gccggtgacg ttggtgcgtt
    12241 cacccacgtt cacaaacagg ctatcgtcgc caatgttcag cggctccagg ccagacaaac
    12301 ggcaggctac gggaatttcc ggcagtttgc gcggcgctaa tccttctact gcacgactca
    12361 tcgctgcaat atgttgcggc gtggtcccgc agcaaccacc gacgatattg agaaagcccg
    12421 cttgcgccca ttcacgtatc tgttttgcca tcgtgtcggc gtcgagatcg tattcaccaa
    12481 aggcgttagg cagcccggcg ttcgggtggg cggtgacgta gcattccgca atccgtgata
    12541 gttcctgcac gtactggcgc agttcatcgg gccccagcgc acagttcagg ccaaaggtca
    12601 gcgcttcggc gtggcgcaat gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc
    12661 gcccggaggc gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cccagcgctt
    12721 caaactccgt tttcaccgca aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt
    12781 caatcaggat cagatccgca ccaccttcca ccagcgcttt ggtggattcg cgataggccg
    12841 ccgccagctg gtcaaaagtg atgttgcgga atgccggatc gttgacgtcc ggagaaatag
    12901 acgccgtgcg gttggttggg ccaagaacac cggcaacgta gcgcggtttc tccggcgtgc
    12961 gcgcggtcca ctcgtcagca caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg
    13021 ccgacaggga ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg
    13081 tttcgatgat atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg
    13141 gtttactgag taccagcagg tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc
    13201 gttcaccacg aaaatcggct tcgttcagtc gatagctctg gatcatggtg cccataccgc
    13261 cgtccaacac cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca
    13321 cacttgctcc cgacacgctt aacccagatt taataaaaat tcaacaaacc atactggaat
    13381 aaacgcatct gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca
    13441 tcggatcagt tcagcaacta ttgcattagc taacaataaa aatgaaaata atttccacga
    13501 tacagaaaaa ggaggctgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg
    13561 ccgttgccac cgcaccagcg actggacagg ttcagtcttt aacgcgtggc ctgaaattac
    13621 tggagtggat tgccgaatcc aatggcagcg tggcactcac agagctggcg caacaagccg
    13681 ggttacccaa ttccacgacc caccgcctgc taaccacgat gcaacagcag ggtttcgtgc
    13741 gtcaggtcgg cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca
    13801 gctttctcca gagccgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg
    13861 aagagtctgg cgaaacggtc aatatggcgg tgcttgatca aagcgatcac gaagcgatta
    13921 ttatcgacca ggtacagtgt acccacctga tgcgaatgtc cgcgcctatc ggcggtaaat
    13981 tgccgatgca cgcttccggt gcgggtaaag cctttttagc ccaactgagc gaagaacagg
    14041 tgacgaagct gctgcaccgc aaagggttac atgcctatac ccacgcaact ctggtgtctc
    14101 ctgtgcattt aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg
    14161 aggaacatgc gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgcgaac
    14221 cgtttgccgc aatttctatt tccggaccga tttcacgtat taccgatgat cgcgtgaccg
    14281 agtttggcgc gatggtgatt aaagcggcga aggaagtgac gctggcgtac ggtggaatgc
    14341 gctgactttt tctggcgggc agaggcaata ttctgcccat catacctgag tggcaataga
    14401 ataagggtgt ctgttaatcg cattgacgcc aaaataactt aatgtcatac acttcacaca
    14461 cttttattaa ctcagcatta tttttaaaca tcaaaccact taattattaa caaaatcgca
    14521 agatctcatt ccaccgaaac ttttacggct gcattatgag attgagcgat ttatgatatt
    14581 tttttagttg taatcatatt aaattttttc actgttctga tagttaaaat tcaagacatc
    14641 aataaacaat gagatattta aatgattact cgtattcctc gtagttcttt ctctgcaaat
    14701 attaataata cagcccagac aaatgaacac caaaccctga gtgaattgtt ttataaagaa
    14761 ctcgaggata aattttctgg caaggagctg gcgacgcctc tattaaaaag cttctcagag
    14821 aactgtagac aaaatgttcg tcatatcttt agcaacaagg attttgtcat taaattttcc
    14881 acgtccgtct tacaagctga taagaaagaa attacgatga ttaataaaaa cgaaaacacg
    14941 acactcactc aaaccattgc tacaatattt gaagaatacc taatggaaat tttacctcaa
    15001 cgctcagaca ctcttgataa acaagaatta aaccaaaaat cagatagaaa agaaaaagaa
    15061 ttcccaagaa ttaaacttaa tggtcaatgt tatttcccgg ggcgccccaa aaccgtatag
    15121 tataccgaca cattgctgca caatatatta atgatattta tcagaatgtt gattacaaac
    15181 cccatcaaga tgattactct tcagctgaaa aatttctcac tcacttcaac aaaaaatgca
    15241 aaaaccagac tttggcgttg atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg
    15301 gtgatttcgg gctagttatg aaagcatatt ttgacaagat ggaatcaaat ggcatcagtg
    15361 ttatggcagc catattactg gtggataacc atgctttgac ggtccggcta agaataaaga
    15421 acacaactga aggatgtacc cattacgtgg tttcggttta tgatcctaat gtaactaacg
    15481 ataaaataag aattatgagc gaaagcaaag aggatattaa acactattct ctgatggatt
    15541 ttatgaatgt agattatagc ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg
    15601 ttgcaataat tccagcactt ccgaaagaac agctattgat gttaaaagga actgtggatg
    15661 aaataacccc tccattatca ccagcaacga tgaatttgct aatggcaatt ggtcagaatc
    15721 accaacttac gcaactgatg attcagctcc agaaaatgcc agaactacat agaacagaaa
    15781 tgttgactgc ctataatagt attaacctcc ccggtttata tttggctata aattatggca
    15841 atgcggatat cgttgagact attttcaatt cattgtcaga aacaggatat gaagggttac
    15901 tctcgaaaaa aaatctcatg catattctgg aggcaaaaga taaaaatggt ttttctggat
    15961 tatttttagc gatatcacgt aaggataaaa atgttgtaac ctcgattctg aacgtcttac
    16021 ctaaactggc cgcaacacat catttagata atgaacaggt gtataaattc ctgagtgcca
    16081 aaaatagaac gtccagccat gttttatacc atgttatggc gaatggtgat gccgacatgc
    16141 tgaaaatttt tttggtcgcg ttacctttgt taattcgcac atgtcatttg actaaagaac
    16201 aggtactcga tctcctgaag gcaaaggatt tttatggttg cccaagacta tacctggcga
    16261 tgcaaaatgg acatagcgat atcgtgaaag ttattctcga agcattgccc tgtctggccc
    16321 aggaaattaa catttcagct tccgatattg tagatcttct gaccgctaaa agtcttgcgc
    16381 gcgacacggg tttgtttatg gccatgcagc gcggacatat gaacgttatt aatactattt
    16441 ttaacgcatt acccactctt tttaatacgt ttaaattcga taaaaaaata tgaagcccct
    16501 cctcctggca aataattcta atgaatatcc cggtttgttt tcagcgatac agcataaaca
    16561 acaaaacgtt gtagagacgg tttatcttgc tttatctgac catgcacgcc tgtttggatt
    16621 taccgctgaa gatattatgg atttttggca acacaaagcg ccacaaaaat actctgcctt
    16681 tgagttggct tttgaatttg gtcaccgggt tattgctgaa ttaatcctta atacattaaa
    16741 taagatggct gaaagctttg gctttacgga taaccctcga tacattgcgg agaaaaatta
    16801 tatggaagct ttactcaaaa aagcatctcc ccataccgta cgctaaatct ttgcctgcgc
    16861 cgatacgcat aaacatcttc cacatgccct tcacgtatgc ggttttgtag tgcgcgccag
    16921 taatcagcgc ggaacaggtc ggcgtgcatc tcttcaaaca gcggaccaat acgcgggtcg
    16981 gcgcatagcc agtggcgaaa ctcttccggg aaaacatcgc ccggcgagac gctgtaccac
    17041 ggttcgctgg caagttcgtc ttccggatag cgcggcggcg ggatgtcgcg gaaattcact
    17101 tccgtcatgt agcaaatttc atcgtaatca taaaaaacca cacgcccgtg acgggtgaca
    17161 ccgaagtttt taaacagcat gtcgccaggg aaaatgttag cagcggcaag ctggcgaata
    17221 gcgttaccgt attcttcaat ggcgtcgcgc aactgctgac cttccacttg ttccagccag
    17281 atattgagcg gcaccatccg ccgctcgatt atagagatgg cgaattacaa tctgctcacc
    17341 aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaata ccggggaaat
    17401 atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc ggcccacacg
    17461 gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca tctctttctg
    17521 cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca gcgtaaacac
    17581 caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac agccctgtag
    17641 ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc cgatagccat
    17701 atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga ccagcgctgc
    17761 gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc
    17821 ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct ggtggatcgg
    17881 cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc aggcggcttt
    17941 attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt tgtctgtccc
    18001 cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat tctgccagcg
    18061 caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc cgtgatcggg
    18121 gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg gctgagagct
    18181 aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac agtacacgga
    18241 gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc gcgtgtaatg
    18301 ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa tgcagcgcag
    18361 ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg
    18421 ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag tcacttcgag
    18481 gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca gtaattccag
    18541 gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc
    18601 cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa gcgtcgcatc
    18661 aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct acagtcagca
    18721 acgtttgtag gttgcttaga actgcgattc ttcagtggag ccagtcagcg cggtgactga
    18781 agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac ccacttcctg
    18841 ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct gctgcacttt
    18901 ctcaacatag tgcttcatac cttcgccctg cgcataagcg tttgccaggt cgaacatgtt
    18961 aaaccacatg ctgtggatac ctgctagggt gatgaactgg aacttgtagc ccatatccga
    19021 cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc agttgaacga
    19081 cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa
    19141 gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt cagcatatgg
    19201 cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc
    19261 ttcactggta cgctcgccgg taataaattc gctgtcatac gggtcgcaat ctgaggtgat
    19321 cagatccgcc gcatcagcat cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc
    19381 agctgccaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca ctaaaacttt
    19441 gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc
    19501 agcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc
    19561 ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc
    19621 aatgcccacg gaccattgga tctgatcggc acgacggaag gtgttgttga tccgctccac
    19681 cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag
    19741 gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac ccgctttcgc
    19801 ctgttgcagc gcctgaccgc cagtcagtgc accgaggttg ttgatgtagc cttttttcga
    19861 ctcaccgtgc agcagacgcc acattttcgc tgcgcccagt tgcgccagcg tgcattcagg
    19921 attgactgaa ccgcgtaatt tcaccacatc ttccgcactg tatgggcgag taatgccttc
    19981 ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat
    20041 gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc
    20101 aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca
    20161 aaacggccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg
    20221 cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa cgttttttga
    20281 tgatggatcc actgccagat cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc
    20341 aggccataaa tcggcacgca gccgttgccg gagatccacg cttcgatgta ctgcacagcc
    20401 acgcgaatgt tagcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc
    20461 tgatcggcag taatcggcgc gtcttgttcg cgcaatactt caagctgatt tttacgggag
    20521 ccgagaatgt cgttgaatac cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat
    20581 gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt gttgagcacc
    20641 tggttattac gctcttcatc tttgctcgga ataaacgcgg ccatgccgcc catcgcaaaa
    20701 gcaccgcgtt tatggcaggt tttaatcagc aggcgtgagt aagcattcag gaatggttta
    20761 tccatcgtca cggcctgtct gtctggcagg acacgatcgg gatagttttt caacgttttg
    20821 atatagctga agatgtaatc ccagcgaccg cagttcagac caacaatatg gtcgcgcagc
    20881 gcgtgaagga tttcgtccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc
    20941 ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac
    21001 caggccgctt cctgctagga ctgggttttc ggcagataga aatagggacc gctgcccttt
    21061 gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg
    21121 attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa
    21181 atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt ggtatagctg
    21241 atggtaccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt gttccagtct
    21301 ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt cagtgcgttg
    21361 atcaccatct tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca
    21421 ggaatcccgc gaattttcca atcagcatcg cgaatggaag ccgtttccga aataaaatca
    21481 ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg
    21541 cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga
    21601 atttgcttct cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc
    21661 tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttcccctct gtgaacgaag
    21721 gaacgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa
    21781 aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat atgaataaaa
    21841 tttattcact tgctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt
    21901 aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat
    21961 gtgccgtaga tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag
    22021 caaattaccg tgactacgcc agctcgctcg cggtttgttt tgcggatcgt tgtgcgggaa
    22081 atagttatac ggtacatccg ggtctagtcc ggcttccaca tcgcgtaaat attcctgcgc
    22141 cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct
    22201 ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc
    22261 acgaatcaac gctgccggaa agtcagcata gcgcgaatgc ggtgccagga atgaatcatc
    22321 aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag
    22381 tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc
    22441 ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat
    22501 ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat
    22561 cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg
    22621 ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg
    22681 caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat
    22741 caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt
    22801 catcacaaag acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg
    22861 aatcggcata acctgattac ctcactacat acgcttatac gtttagacat ccagatagct
    22921 gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa
    22981 ggaaaaggct acgttagaat ataagaatga cgaaaaggag agaaaatggt tattagtatt
    23041 cgccgctcac ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat
    23101 gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct ggtgcgttcc
    23161 tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggagcagcc ggttgggttt
    23221 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc
    23281 ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac
    23341 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
    23401 ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg
    23461 ggggaataag ccgcctctgc cgtgcctgcc caagctggag aagacg
//
LOCUS       DAJVPT010000063        22956 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVPT010000063 DAJVPT010000000
VERSION     DAJVPT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            90..866
                     /gene="exuR"
                     /locus_tag="OQM47_002402"
     CDS             90..866
                     /gene="exuR"
                     /locus_tag="OQM47_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1480523.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1211..1873
                     /locus_tag="OQM47_002403"
     CDS             1211..1873
                     /locus_tag="OQM47_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1480524.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1877..2260
                     /gene="mzrA"
                     /locus_tag="OQM47_002404"
     CDS             1877..2260
                     /gene="mzrA"
                     /locus_tag="OQM47_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1480525.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2407..2761
                     /locus_tag="OQM47_002405"
                     /pseudo
     CDS             2407..2761
                     /locus_tag="OQM47_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2799..3104
                     /locus_tag="OQM47_002406"
     CDS             2799..3104
                     /locus_tag="OQM47_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1480526.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3107..3511
                     /locus_tag="OQM47_002407"
     CDS             3107..3511
                     /locus_tag="OQM47_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1480527.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3501..3800
                     /locus_tag="OQM47_002408"
     CDS             3501..3800
                     /locus_tag="OQM47_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1480528.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3986..4282
                     /locus_tag="OQM47_002409"
     CDS             3986..4282
                     /locus_tag="OQM47_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1480529.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4352..5338
                     /gene="yqjG"
                     /locus_tag="OQM47_002410"
     CDS             4352..5338
                     /gene="yqjG"
                     /locus_tag="OQM47_002410"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1480530.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5631..5999
                     /locus_tag="OQM47_002411"
     CDS             5631..5999
                     /locus_tag="OQM47_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1480531.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6206..7102)
                     /gene="yhaJ"
                     /locus_tag="OQM47_002412"
     CDS             complement(6206..7102)
                     /gene="yhaJ"
                     /locus_tag="OQM47_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1480532.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7207..7908
                     /locus_tag="OQM47_002413"
     CDS             7207..7908
                     /locus_tag="OQM47_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1480533.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7931..8095
                     /locus_tag="OQM47_002414"
     CDS             7931..8095
                     /locus_tag="OQM47_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480534.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8307..9617)
                     /locus_tag="OQM47_002415"
     CDS             complement(8307..9617)
                     /locus_tag="OQM47_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1480535.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9645..10976)
                     /locus_tag="OQM47_002416"
     CDS             complement(9645..10976)
                     /locus_tag="OQM47_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1480536.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11251..12615)
                     /gene="tdcG"
                     /locus_tag="OQM47_002417"
     CDS             complement(11251..12615)
                     /gene="tdcG"
                     /locus_tag="OQM47_002417"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1480537.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12687..13076)
                     /locus_tag="OQM47_002418"
     CDS             complement(12687..13076)
                     /locus_tag="OQM47_002418"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1480538.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13090..15384)
                     /gene="tdcE"
                     /locus_tag="OQM47_002419"
     CDS             complement(13090..15384)
                     /gene="tdcE"
                     /locus_tag="OQM47_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1480539.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15418..16626)
                     /gene="tdcD"
                     /locus_tag="OQM47_002420"
     CDS             complement(15418..16626)
                     /gene="tdcD"
                     /locus_tag="OQM47_002420"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1480540.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16652..17983)
                     /gene="tdcC"
                     /locus_tag="OQM47_002421"
     CDS             complement(16652..17983)
                     /gene="tdcC"
                     /locus_tag="OQM47_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1480541.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18005..18994)
                     /gene="tdcB"
                     /locus_tag="OQM47_002422"
     CDS             complement(18005..18994)
                     /gene="tdcB"
                     /locus_tag="OQM47_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1480542.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19093..20031)
                     /gene="tdcA"
                     /locus_tag="OQM47_002423"
     CDS             complement(19093..20031)
                     /gene="tdcA"
                     /locus_tag="OQM47_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1480543.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20221..20565
                     /gene="tdcR"
                     /locus_tag="OQM47_002424"
     CDS             20221..20565
                     /gene="tdcR"
                     /locus_tag="OQM47_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1480544.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20821..21361
                     /locus_tag="OQM47_002425"
                     /pseudo
     CDS             20821..21361
                     /locus_tag="OQM47_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21383..21730
                     /locus_tag="OQM47_002426"
     CDS             21383..21730
                     /locus_tag="OQM47_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480545.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21899..22549
                     /locus_tag="OQM47_002427"
     CDS             21899..22549
                     /locus_tag="OQM47_002427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480546.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6194 a         5786 c         5290 g         5686 t
ORIGIN      
        1 tcaatcatca aatttatata tgaccaatgg catacccatg aataaagtgg tatgataact
       61 tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc cgtttgtatc
      121 aacaacttgc tgctgacctg aaagagcgca ttgaacaggg cgtctatctg gtgggtgata
      181 aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg gtagttcgtg
      241 aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt tcaggtattc
      301 atgtggtttc caaccagccg cgtcatcagc aggcggctga caataatatg gaattcgcca
      361 attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat attgccgagt
      421 tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt caggaacagg
      481 cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac attcaggtag
      541 ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg acccagcgta
      601 gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc gttgataact
      661 ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatcct catgctgcta
      721 agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac gaaaccagcg
      781 acgatttcga gttcaatgcc gaccgctatc tgttcgccga aaacccggta gtgcatctcg
      841 atacggccac cagcggcagc aaatgaaatt cctgtccgac aggcgctgca atgcgcctgt
      901 ttgtctcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc
      961 tcccctgcaa tcagcaaaat cagactccac ggacatgtaa ttttgataac gaacaacgtt
     1021 gacctttgtt acaattagat tcaatttgaa tttatgcttt tgaatgcttt cttatctcac
     1081 gttttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa
     1141 cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac cgattcacca
     1201 ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc aggattttga
     1261 aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg taattttgtt
     1321 ccttgaaaat ggcttgcttc cggcggcctt tttaccgggc gacagtttac tggtattggt
     1381 cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt
     1441 tgccgccagc ctcggttgct gggtcagcta tattcagggg cgatggctgg gcaatacccg
     1501 caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg cacaccatct
     1561 ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcat ttgtcagaac
     1621 actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc agttcttcaa
     1681 ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca tgctcggcaa
     1741 aacgccggta tttttaaagt acgaggacca gctgatgtca tgcctgatgc tgctcccggt
     1801 ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg
     1861 aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag cctggtccgg
     1921 cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc gccagcaaga
     1981 gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg gtttttcgat
     2041 ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca aaaacgacac
     2101 tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag
     2161 aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg ctatgcagtg
     2221 gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta tccgaaacat
     2281 atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg tggttaacac
     2341 taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac aatggaaggt
     2401 tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag tgccggtagt
     2461 tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat cagctatgcc
     2521 gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag tgaagtccgg
     2581 gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa
     2641 gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg cgatgccgat
     2701 agaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta
     2761 actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg
     2821 aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat
     2881 ctggcgagaa gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga
     2941 aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg
     3001 cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg
     3061 ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca
     3121 tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat
     3181 ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc
     3241 taatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct
     3301 tatgagcctg atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat
     3361 gattgccacc accgtggtgt tgctgctact ggcactgatt ggcggtatct ggacgttacg
     3421 taaatcgcgt aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca
     3481 actgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac
     3541 tgcttagcca gatccagcaa caacggctgg atatttccgc cagtagtcgt gaatggctgg
     3601 aggcaacagg cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc
     3661 tggttggcag tagagtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct
     3721 gggccagacg cggttttggc gtatggagcg cctggcgtct ggttaaaacg accctcaagc
     3781 agcaacagct tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccatcaaa
     3841 ttttttgaat atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat
     3901 tctctccatc gacagcaacg acgctaatag cgcgcccttg cacaaaaaaa caatcagcag
     3961 cctgagtggc gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc
     4021 gcacatttta atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc
     4081 tggtacccaa caatatatgg aagcaatggg tgtcccgggt tttatgctgc tgacggcatt
     4141 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     4201 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcggccg
     4261 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     4321 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     4381 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     4441 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     4501 agagaaagat cgttatcatc tctatgtttc actcgcctgc ccgtgggcgc accgcacgct
     4561 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     4621 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     4681 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     4741 agttactgtt cccgtgctgt gggacaaaaa gaaccacacc atcgtcagca acgaatcagc
     4801 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcaaaag cgggtgatta
     4861 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     4921 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     4981 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5041 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5101 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     5161 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     5221 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     5281 aattggtccg tggcaggatc tcgatgaacc gcatggacga gatgttcgct tcggttaaaa
     5341 aaaggcacct tacgaggtgc ctctgataat tagctaagtg catgttgttc atgtcggatg
     5401 cggcgtgaac gccttatcaa acctacaaag cgtttatatt caatatgtta caatgaccgc
     5461 gtaggccaga taagcgtagc gcatctggcg attttgcgct tgccatcagt cttagcgcgc
     5521 cttcacggca ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     5581 tcttaatttg ctgctttaaa aacaagtgat tgagcaaaat tgaggcaaaa atggactggt
     5641 atctgaaagt actaaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact
     5701 ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg gataaaatgt
     5761 taggctggca acgcgctggt ggcgaaggca tcctgacgat aatctacggt attctggtgt
     5821 ttttaccgtg gtgggcggtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt
     5881 gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc tttaactgcc
     5941 aggcgggtac gccaggcgaa aaccgctttg gaccagatcc aaaactggaa caagaataat
     6001 gattaagcac cttgtaaaag gtgcttcaaa ttgttgacaa agtgcgcttt gtttatgccg
     6061 gatgcggcgt aaacgcctta tccagcctac aaattgccaa aattcaatat attgcagtga
     6121 ctgcgtaggc ctgataagcg tagcgcatca ggcagttttg cgtttgtcag cagtcagaag
     6181 caccttgtga aaggtgctta atagattatt ttccagcgaa caactttgga atttcccgca
     6241 gacaccagga tttcgcttcc cccatactgt cacgacgcca ggccataata atatcgatct
     6301 cgctggtcga ttccgggctg acgacacgca accgcccttc cgcaatatct ttttcgacca
     6361 tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt ttatcttcaa
     6421 tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg tacggtcaac accgggcgct
     6481 cacgagcggt atccgctacc gcaattccac gatatttcac acgcgtcact tcagataacg
     6541 gttccggctc ctgatgaatc gggtgatcag gcgcggcaac gtagacgttc attaacgtat
     6601 agagcttgcg cgagttgatc tccgacgagg aacgaaaatg catatccggc gcgataacga
     6661 tatccgcccg cccctgctcc agccgttccc acgcccccgc cagcacttct gtgatgattg
     6721 ccagttgggt attggctttt gccgccagtt tgtcgattaa cgggaaaaag gcaggtgtcg
     6781 gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg agagcttccg
     6841 catcagtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc agcaacatcc
     6901 tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc acatccagct
     6961 cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc agctcatccg
     7021 ctgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt gcttccagcg
     7081 ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat accgggcaga
     7141 atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa agagaggtca
     7201 agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta cggatggttg
     7261 caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt aggctatgcc
     7321 tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca gccgcgaacc
     7381 tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta tcgcgacagc
     7441 gaaggcaatc atgttcaggc cagcgccgga gaggcgttgc tgctctctac ccagccgggt
     7501 gtcagctata gcgaacacaa tctcagcaaa gacaaaccgt taacgcgaat gcagctttgg
     7561 ctggatacct gcccgcagcg agagaatccg ctgattcaaa agctggcgct taatatggac
     7621 aagcagcaat taatcgcctc gccagagggt gcgatgggaa gcctgcaatt acgccaacaa
     7681 gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca gttgcatggg
     7741 ccacgcgcgt atttgcaatc gattcacggg aaatttcatg cgcttacgca tcatgaagag
     7801 aaagcggcgc taacctgcgg tgatggggcg tttattcgtg acgaggctaa cattacgctg
     7861 gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt gtttttacag
     7921 ggagatgatg atgagtaaaa aatcgaccaa aaagcgccag ccagcgaagc ccgtggtggc
     7981 gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga gcgagctgga
     8041 agctatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg cgtaaagaca
     8101 ggttgccgga tgcggcgtaa acgccttatc cggcctacaa aacacaacaa catcaacaaa
     8161 ttaaacaccc cgtaggccgg ataagatgcg ccagcatcgc atccggcatc aggtacaaaa
     8221 cacaacaaca tcaacaagtt gagcaccccg taggccggat aaggtgcgcc agcatcgcat
     8281 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     8341 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     8401 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     8461 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     8521 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     8581 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     8641 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     8701 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     8761 accaccacag gcattattgc agtgatcccc tggttacccg agccggagtt actcatagcc
     8821 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
     8881 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
     8941 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
     9001 tcgagaataa agcggatcgc cgcaaatggg acttcattga cgaacttcag gatctcagcc
     9061 agcgtcgttc tggaaagcac cgtcagcggc gactcttgct cgccctctgc cacacacgcc
     9121 tggtgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt atgcccgccg
     9181 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
     9241 tcattgcaag gttcctggat cttaacagag actttccccg ccgccagcag tgctttggca
     9301 tcggtaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
     9361 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
     9421 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
     9481 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
     9541 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
     9601 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
     9661 aacagagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
     9721 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
     9781 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatggcg
     9841 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
     9901 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
     9961 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    10021 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    10081 gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag cgtgaacgac
    10141 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    10201 gctttatgcc gcgccacttc aattgatttt tcccgcgagc gataagagat caccatcggg
    10261 cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    10321 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    10381 atcgacacgc ccagcgcagc gactaccagc agcttggtca gcaccatgtc ggtcgaaatt
    10441 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    10501 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    10561 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    10621 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    10681 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    10741 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    10801 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    10861 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    10921 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat ttccataatt
    10981 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    11041 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    11101 aaatcttcac aatcaagact ttaaaataac ggagatggat aagaattttc tactaaatta
    11161 atcacaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    11221 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    11281 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    11341 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc
    11401 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    11461 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    11521 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgctgcca ttgaacaggc
    11581 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    11641 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    11701 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    11761 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    11821 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    11881 acgacgcagc gctacggcac gacacggcac attgagcgga ccaggcagca cgccttcagt
    11941 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa accctgcgtc
    12001 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    12061 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    12121 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    12181 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    12241 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    12301 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    12361 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    12421 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac
    12481 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    12541 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    12601 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    12661 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    12721 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    12781 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    12841 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    12901 gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg
    12961 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    13021 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    13081 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    13141 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    13201 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    13261 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    13321 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    13381 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    13441 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    13501 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    13561 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    13621 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    13681 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    13741 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    13801 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    13861 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    13921 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    13981 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    14041 gagcagcgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    14101 cattgggctg acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag
    14161 atcgtcattt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    14221 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    14281 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    14341 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    14401 aaattccggt gtacgcagga agcgtaccat acggatcttc atgatgaagt gatcgatcag
    14461 ttcctgtgcc tgctgctcat tgaggacgcc agctttaaag tcgcgctcaa tgtagatatc
    14521 caggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc
    14581 cagataagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg ggcgagagat
    14641 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    14701 cagctcctca cgcagacgga tggtggcttc cagatcctcg cctttttcca gacgagactg
    14761 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    14821 gcggcgatag tcaccgataa tgcgcccacg accatagcca tctggtaaac cggtcagcac
    14881 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    14941 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    15001 gtggaatgaa cttttaatca tattaatgcc accgaacggg tgtagcgcac gtttcaacgg
    15061 cgcatccgtt tgcaggccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    15121 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    15181 gatacggatg ccttccatta ccttttccca caattccgtg gtggcaggcg tcgcttcggc
    15241 gagaaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    15301 aatttcgctt ttccagtccg tacctttaaa gccaagccat gcgtcagcgt acagcttatc
    15361 gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta
    15421 tgcaaattct gcgggcgcgt taactttacc taaatgaatg gcatccaaag caatcatttt
    15481 ttcttcgtta gtcggaataa cgacgcaaat gacatgcgca ttttcactgg aaacaattcg
    15541 ctcgccaaaa gagttagaac gattattcat ttctgtatca atctctacgc ctaatacagc
    15601 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    15661 tataattcca tccagccgac gtaatgaagc tgcatgtccg gcaatatgac gggcaattcg
    15721 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    15781 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    15841 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    15901 aaagtcgaca tcgccactgc gggtacccat catcaagcct tccagcggcg tcattcccat
    15961 tgaggtgtca acactctgac cgttgcgaac tgcgcagatt gacgcgccat tgccaagatg
    16021 cgccacaacc aggccggagt catcttccgc cagattcagc agcgaatgtg cgcgctggga
    16081 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    16141 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    16201 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    16261 atttgcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    16321 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    16381 taaggccacg ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    16441 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    16501 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacaat cgctggcatc
    16561 gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    16621 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    16681 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    16741 gagacggcgc tttacggata gcatacatcg gcaacaggca cagcagggat gcgataatcg
    16801 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    16861 tggagcccat gatgaagatc atgctgagag tattcagttt acccagcgac actttggttt
    16921 tgtcaccttt ataaccgaac ttcagaatca gaccattcaa gccttccagc gtccccagat
    16981 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    17041 gtgtaatcgc gaacgttgtt ttcgtaccgg tcatggacgc aaagtggtta gccagataag
    17101 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agagtaaaca
    17161 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    17221 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    17281 tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa
    17341 tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    17401 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    17461 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    17521 accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg
    17581 gtgcaaatcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    17641 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    17701 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    17761 gacacagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    17821 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    17881 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    17941 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    18001 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    18061 cggaaataat actgacggtt tttctatttt gaatatattg gtctaattta ccgcttaata
    18121 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    18181 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    18241 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    18301 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    18361 tttctgactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    18421 taccagcaat taaaccacca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    18481 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    18541 gtgggataaa aatacgacct tccatttcga caatttcgct cactttagcg atagtgtcgt
    18601 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    18661 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    18721 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    18781 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    18841 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    18901 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    18961 caatagcaac cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    19021 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    19081 aaaaacaaac aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    19141 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    19201 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    19261 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    19321 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    19381 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    19441 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    19501 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    19561 ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    19621 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    19681 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    19741 caccaccgct tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    19801 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    19861 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    19921 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    19981 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    20041 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaataaacag gatatgtgcg
    20101 accactcaca aattaacttt cattactttc caaggtatcg ttattacaat taaacatctt
    20161 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgattttta tcaaaaaaaa
    20221 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
    20281 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
    20341 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
    20401 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
    20461 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
    20521 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
    20581 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct ggtcgccgcg
    20641 ataaggtcag acgaagtcaa caagggggat tttcactgtg ctttacatca gcgtaggaca
    20701 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgtaa
    20761 gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
    20821 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
    20881 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt
    20941 gtctccacca atgacgttaa taaaatttac agtataagta atgaactccg gagagtatta
    21001 tctgcaatta ctgcattgaa tttctatcaa ggcgatgttc cttctgtcat gattccgaat
    21061 ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac atgatgatta
    21121 tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat gtacttgctc
    21181 agccgtcaat aaaaaagagc tggaatgtat taaggagacg atttctaagt attgcgcaaa
    21241 gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa cctgcattac
    21301 ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg atttttattg
    21361 attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg atattagtag
    21421 tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa ataacctcga
    21481 agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg tcactttact
    21541 taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa aagaattagc
    21601 gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt ttttaggtta
    21661 taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta atgaaccgtt
    21721 aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa aagatattta
    21781 caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata tagttgatct
    21841 atcctctctg gacttacgta atgtcgattt agattattat gatttcacag ataaacatat
    21901 ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta atgccaacat
    21961 gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg attatttact
    22021 aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa aaatcaagtt
    22081 tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa aaattatgag
    22141 atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag aaaatgaatt
    22201 aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca gaattaaatt
    22261 accagagatg aaagaattct catcaaaaaa aacatatgat tacatcgata caatcattga
    22321 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    22381 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    22441 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    22501 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    22561 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    22621 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    22681 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    22741 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    22801 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    22861 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
    22921 cgaagcgggt ttttacgtat atcaggtgaa actgac
//
LOCUS       DAJVPT010000064        22268 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVPT010000064 DAJVPT010000000
VERSION     DAJVPT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1015)
                     /locus_tag="OQM47_002428"
     CDS             complement(<1..1015)
                     /locus_tag="OQM47_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480547.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAP"
     gene            complement(1117..2097)
                     /locus_tag="OQM47_002429"
     CDS             complement(1117..2097)
                     /locus_tag="OQM47_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1480548.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2105..2815)
                     /locus_tag="OQM47_002430"
     CDS             complement(2105..2815)
                     /locus_tag="OQM47_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1480549.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OQM47_002431"
     CDS             complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OQM47_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1480550.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5228..6637
                     /gene="glnA"
                     /locus_tag="OQM47_002432"
     CDS             5228..6637
                     /gene="glnA"
                     /locus_tag="OQM47_002432"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1480551.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6810..7859
                     /gene="glnL"
                     /locus_tag="OQM47_002433"
     CDS             6810..7859
                     /gene="glnL"
                     /locus_tag="OQM47_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1480552.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7871..9280
                     /gene="glnG"
                     /locus_tag="OQM47_002434"
     CDS             7871..9280
                     /gene="glnG"
                     /locus_tag="OQM47_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1480553.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9392..9502
                     /locus_tag="OQM47_002435"
     CDS             9392..9502
                     /locus_tag="OQM47_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1480554.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OQM47_002436"
     CDS             complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OQM47_002436"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1480555.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OQM47_002437"
     CDS             complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OQM47_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1480556.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12384..13016
                     /locus_tag="OQM47_002438"
     CDS             12384..13016
                     /locus_tag="OQM47_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1480557.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OQM47_002439"
     CDS             complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OQM47_002439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1480558.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16548..17480
                     /locus_tag="OQM47_002440"
     CDS             16548..17480
                     /locus_tag="OQM47_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1480559.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17521..18951)
                     /locus_tag="OQM47_002441"
     CDS             complement(17521..18951)
                     /locus_tag="OQM47_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1480560.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OQM47_002442"
     CDS             complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OQM47_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1480561.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OQM47_002443"
     CDS             complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OQM47_002443"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1480562.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20812..21081)
                     /locus_tag="OQM47_002444"
     CDS             complement(20812..21081)
                     /locus_tag="OQM47_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1480563.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21151..21735
                     /gene="mobA"
                     /locus_tag="OQM47_002445"
     CDS             21151..21735
                     /gene="mobA"
                     /locus_tag="OQM47_002445"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1480564.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21717..22244
                     /gene="mobB"
                     /locus_tag="OQM47_002446"
     CDS             21717..22244
                     /gene="mobB"
                     /locus_tag="OQM47_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1480565.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5512 a         5580 c         5330 g         5846 t
ORIGIN      
        1 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
       61 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
      121 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
      181 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
      241 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
      301 tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatggttt
      361 tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct tttttcacct
      421 caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg cctacggcaa
      481 tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca ctgccaaacc
      541 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
      601 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
      661 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
      721 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
      781 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc
      841 ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
      901 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
      961 taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat
     1021 cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatatg
     1081 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc
     1141 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc
     1201 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat
     1261 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccgacgcata
     1321 gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cgccaaggca
     1381 gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa tacaggcttt
     1441 atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat
     1501 atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttagc
     1561 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccagctc
     1621 atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt cctgatacgt
     1681 ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc
     1741 ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg cgacggcttc
     1801 gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt tcagacggcg
     1861 ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
     1921 gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga tatcaagtaa
     1981 atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata
     2041 aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
     2101 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
     2161 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
     2221 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
     2281 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
     2341 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
     2401 ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggcgtca
     2461 ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc tcggtaaacc
     2521 cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
     2581 gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta cggaagattt
     2641 tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta atgcccagca
     2701 gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg ccatctttca
     2761 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc gccattgttt
     2821 aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa gaaagcaatt
     2881 aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta atcacagact
     2941 tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa cctgccatcg
     3001 ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc
     3061 ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt
     3121 ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg
     3181 gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg
     3241 tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt
     3301 aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga
     3361 acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc tgacccactt
     3421 cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag
     3481 tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga
     3541 gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca
     3601 gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg
     3661 gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat accgccagtt
     3721 cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca ccacgaccag
     3781 aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca
     3841 gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct tctttaccgc
     3901 agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg
     3961 ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca
     4021 ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac
     4081 cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg ctatcgatga
     4141 tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg ccgataacgc
     4201 caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa
     4261 cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg tcttccgcca
     4321 tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt
     4381 ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc
     4441 aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt
     4501 aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa
     4561 ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg
     4621 cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt
     4681 tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt
     4741 gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg
     4801 ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt
     4861 tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg
     4921 atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag
     4981 cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag
     5041 cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga
     5101 taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct tttttacggc
     5161 gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa tccaggagag
     5221 ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt
     5281 tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc ctgctcatca
     5341 ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg
     5401 gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg cagtgattga
     5461 cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct
     5521 gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt acctgcgttc
     5581 cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga
     5641 catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc
     5701 atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg
     5761 cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg aaatgtgtct
     5821 ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg
     5881 tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat
     5941 ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc
     6001 aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa
     6061 cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgtacta
     6121 cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa
     6181 ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcgcg
     6241 taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga
     6301 agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc
     6361 cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta
     6421 tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc
     6481 actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcacggatga
     6541 agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc
     6601 gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc
     6661 gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     6721 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     6781 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     6841 agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg gcgatccatt
     6901 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacgc
     6961 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     7021 aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt cgctcgcata
     7081 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
     7141 tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa gtagctgccc
     7201 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     7261 cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga
     7321 ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg ccgcagctgc
     7381 ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta acgctggtgt
     7441 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac
     7501 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
     7561 aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga
     7621 ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg
     7681 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     7741 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
     7801 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
     7861 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     7921 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
     7981 ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     8041 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc
     8101 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     8161 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg
     8221 cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta acggcccaac
     8281 gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta tcggtcggct
     8341 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     8401 cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg cgctgaatat
     8461 ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt
     8521 tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
     8581 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     8641 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     8701 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
     8761 gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga
     8821 tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
     8881 gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt
     8941 gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt
     9001 gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
     9061 gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc tgcgttccgg
     9121 tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc
     9181 gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     9241 cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
     9301 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
     9361 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
     9421 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     9481 gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga aagtcgtagg
     9541 cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg atgcggcgta
     9601 aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc cagcgcatca
     9661 ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc
     9721 cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg
     9781 tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc
     9841 ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt
     9901 ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg taatccagac
     9961 ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg
    10021 caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac tgcttcaact
    10081 ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca
    10141 gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac
    10201 gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg ataaactgat
    10261 agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac
    10321 tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt
    10381 agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga
    10441 aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac
    10501 caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa
    10561 ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt
    10621 cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa
    10681 tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga cggctgattt
    10741 gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc
    10801 cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg
    10861 ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc
    10921 agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta
    10981 aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc
    11041 gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag
    11101 acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt
    11161 tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac
    11221 gaataaccac accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac
    11281 ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc
    11341 ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg
    11401 atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag
    11461 acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact ccgcctgcgg
    11521 tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcactcc
    11581 caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct
    11641 gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt
    11701 ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt
    11761 tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc
    11821 cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc
    11881 gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct
    11941 tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt tgattccatt
    12001 tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg
    12061 gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct
    12121 actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga
    12181 gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat cgcagaattt
    12241 tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt
    12301 acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc
    12361 tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga
    12421 tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag
    12481 gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg
    12541 ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg
    12601 gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc
    12661 gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg
    12721 tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg
    12781 cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg
    12841 gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg
    12901 tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg aggcagaaac
    12961 tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt
    13021 cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa
    13081 ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca
    13141 ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt
    13201 acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact
    13261 ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata
    13321 ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg
    13381 gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc
    13441 agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg
    13501 gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata
    13561 cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata
    13621 atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga
    13681 cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca
    13741 acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg
    13801 tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc
    13861 cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta
    13921 cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc
    13981 cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg cgagagatgc
    14041 gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc
    14101 gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg
    14161 ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc
    14221 acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgacttcagc
    14281 ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct
    14341 tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata
    14401 ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt aaattcttca
    14461 cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag ctcttcagaa
    14521 tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc
    14581 ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg tttctgcaga
    14641 tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt
    14701 ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc
    14761 tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga
    14821 cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc
    14881 agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc
    14941 agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc gtgagagatt
    15001 tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac gcctggctcg
    15061 atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta
    15121 tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca
    15181 tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg
    15241 tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc ctgtaaccat
    15301 ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccccaac
    15361 aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaaca
    15421 tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt ttgctcgagc
    15481 ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca
    15541 tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg
    15601 cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc
    15661 aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc
    15721 atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg
    15781 ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg
    15841 tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc
    15901 agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt
    15961 tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc
    16021 ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc
    16081 ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat
    16141 gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt
    16201 tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt
    16261 ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt ggataacttt
    16321 gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag catgatgttt
    16381 gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga taatgggatt
    16441 ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt
    16501 cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg gctaaccttt
    16561 tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat
    16621 tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac
    16681 tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt
    16741 attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag
    16801 ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta
    16861 gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat
    16921 atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg
    16981 atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg
    17041 gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca
    17101 ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca
    17161 cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg
    17221 gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc
    17281 cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg gatttacagc
    17341 ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc
    17401 agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct ctcatgtcat
    17461 cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg agtgagtatt
    17521 ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt
    17581 aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt tgtcaatcgt
    17641 gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat cagcctgctt
    17701 ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac
    17761 gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt ttgtggatga
    17821 tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc
    17881 gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata
    17941 ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg
    18001 gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt ggcgcactgc
    18061 tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc
    18121 ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac
    18181 gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt
    18241 gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc
    18301 taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa
    18361 ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc
    18421 accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg caggggcaac
    18481 atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga
    18541 tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt
    18601 tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc
    18661 gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc
    18721 accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt tgtcaaagtt
    18781 tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat cgttaaaatc
    18841 tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc
    18901 ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt
    18961 ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcggcag
    19021 gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg tgaatattca
    19081 cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc accgtatcag
    19141 catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga ttcagctgat
    19201 atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct gcggcttttt
    19261 cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg tactcttcac
    19321 ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga atggtctggg
    19381 ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc agcgccatcg
    19441 ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag ttgacgtggt
    19501 atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa atatgcagaa
    19561 cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc acttgcggcg
    19621 cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac tgcgccgccg
    19681 atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc atgatctact
    19741 ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac ttttgcctgt
    19801 tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca cgggaaattt
    19861 ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata aaccaaacgc
    19921 atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct aaattcttca
    19981 taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc attgagcaac
    20041 atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac aaacattgga
    20101 ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag aacggtgaaa
    20161 tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt cagaaatgcc
    20221 gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg ctcaatgaga
    20281 tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc cgtctggtgc
    20341 atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc ttcgaactgg
    20401 cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa taaagtctga
    20461 ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg caacgcaaat
    20521 tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa tttgacgaca
    20581 aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct gttaagcggg
    20641 gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat ggtatccggg
    20701 tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa acattacgaa
    20761 tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct tttactcttt
    20821 aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat ctttctgcaa
    20881 acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga tcagaatgtc
    20941 atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa
    21001 ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt caataacttc
    21061 attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca cgttagcagg
    21121 gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat aacaggcgtt
    21181 gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt gctggactta
    21241 aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct ctctcacgtc
    21301 gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa
    21361 gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat gcagcaggaa
    21421 gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca tgatttagcc
    21481 gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc
    21541 gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact ggaatatctg
    21601 caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca tgcggttgat
    21661 ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct tgcccgatgg
    21721 caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta
    21781 cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg gggctgatta
    21841 agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat gagctgcgca
    21901 aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg atgacggaaa
    21961 caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat acctcaaagc
    22021 tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt gtgctgtttc
    22081 gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt attgctgtag
    22141 ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat gttgaggggc
    22201 tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga gtaggctgga
    22261 tcagacgc
//
LOCUS       DAJVPT010000065        22137 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVPT010000065 DAJVPT010000000
VERSION     DAJVPT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..2373
                     /locus_tag="OQM47_002447"
     CDS             <1..2373
                     /locus_tag="OQM47_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCS1480566.1"
                     /translation="TIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPAFKQL
                     DKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINALLLG
                     YSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSY
                     LQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKS
                     NAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSV
                     PLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAA
                     MFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRY
                     STSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYG
                     SFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFS
                     LPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQG
                     YNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQS
                     LGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDL
                     ENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDD
                     DGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2364..3347
                     /locus_tag="OQM47_002448"
     CDS             2364..3347
                     /locus_tag="OQM47_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1480567.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3445..3985
                     /locus_tag="OQM47_002449"
                     /pseudo
     CDS             3445..3985
                     /locus_tag="OQM47_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3993..4508
                     /locus_tag="OQM47_002450"
     CDS             3993..4508
                     /locus_tag="OQM47_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1480568.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4474..5211
                     /locus_tag="OQM47_002451"
     CDS             4474..5211
                     /locus_tag="OQM47_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1480569.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5322..6332
                     /gene="pyrD"
                     /locus_tag="OQM47_002452"
     CDS             5322..6332
                     /gene="pyrD"
                     /locus_tag="OQM47_002452"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1480570.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6506..7048
                     /gene="zapC"
                     /locus_tag="OQM47_002453"
     CDS             6506..7048
                     /gene="zapC"
                     /locus_tag="OQM47_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1480571.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7045..8154)
                     /gene="ycbX"
                     /locus_tag="OQM47_002454"
     CDS             complement(7045..8154)
                     /gene="ycbX"
                     /locus_tag="OQM47_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1480572.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8398..10506
                     /gene="rlmKL"
                     /locus_tag="OQM47_002455"
     CDS             8398..10506
                     /gene="rlmKL"
                     /locus_tag="OQM47_002455"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1480573.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10518..12425
                     /locus_tag="OQM47_002456"
     CDS             10518..12425
                     /locus_tag="OQM47_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1480574.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12555..13808
                     /gene="pqiA"
                     /locus_tag="OQM47_002457"
     CDS             12555..13808
                     /gene="pqiA"
                     /locus_tag="OQM47_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1480575.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13813..15453
                     /gene="pqiB"
                     /locus_tag="OQM47_002458"
     CDS             13813..15453
                     /gene="pqiB"
                     /locus_tag="OQM47_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1480576.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15450..16013
                     /gene="pqiC"
                     /locus_tag="OQM47_002459"
     CDS             15450..16013
                     /gene="pqiC"
                     /locus_tag="OQM47_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1480577.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16269..16436
                     /gene="rmf"
                     /locus_tag="OQM47_002460"
     CDS             16269..16436
                     /gene="rmf"
                     /locus_tag="OQM47_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1480578.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16506..17024)
                     /gene="fabA"
                     /locus_tag="OQM47_002461"
     CDS             complement(16506..17024)
                     /gene="fabA"
                     /locus_tag="OQM47_002461"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1480579.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17093..18853)
                     /locus_tag="OQM47_002462"
     CDS             complement(17093..18853)
                     /locus_tag="OQM47_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1480580.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19038..19490
                     /gene="matP"
                     /locus_tag="OQM47_002463"
     CDS             19038..19490
                     /gene="matP"
                     /locus_tag="OQM47_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1480581.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19566..20612)
                     /gene="ompA"
                     /locus_tag="OQM47_002464"
     CDS             complement(19566..20612)
                     /gene="ompA"
                     /locus_tag="OQM47_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1480582.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20968..21477)
                     /gene="sulA"
                     /locus_tag="OQM47_002465"
     CDS             complement(20968..21477)
                     /gene="sulA"
                     /locus_tag="OQM47_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1480583.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21696..22106
                     /gene="sxy"
                     /locus_tag="OQM47_002466"
                     /pseudo
     CDS             21696..22106
                     /gene="sxy"
                     /locus_tag="OQM47_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5649 a         5205 c         5802 g         5481 t
ORIGIN      
        1 acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa ggggattgcc
       61 gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc gtttccggct
      121 tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc ggatgccagc
      181 gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat cgccatcaaa
      241 agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa cgcgctatta
      301 ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc
      361 gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag attacgcaac
      421 aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct cagcagctat
      481 ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga ttatactgca
      541 ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg
      601 ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc
      661 cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct
      721 tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa
      781 gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc attactccag
      841 cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa
      901 cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg gggaacgaca
      961 tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt
     1021 aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag tacgctggca
     1081 gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa
     1141 ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt
     1201 tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat
     1261 acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc
     1321 aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag ccagcaaact
     1381 tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac gcaaattaaa
     1441 gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc tgatgctgat
     1501 caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag aagtaatgat
     1561 agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa
     1621 gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc
     1681 tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag cgccagcatg
     1741 gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga taacggcagt
     1801 caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc
     1861 ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc agagaatact
     1921 cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt tgtcccctat
     1981 gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa acgtaacgtg
     2041 gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt tctggcggag
     2101 ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg tataccgctg
     2161 cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat tattgatgat
     2221 gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt acgctggggc
     2281 gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca aattaactct
     2341 gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc gtgttattac
     2401 tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct gcttatatcg
     2461 ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc gttaaaactc
     2521 tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag tggaacgaat
     2581 cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt ggccgatggg
     2641 cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa attaatgact
     2701 atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg gttcctttcg
     2761 aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc ggtcaacgtt
     2821 taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata ttgcagccgt
     2881 tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa tgctacaaca
     2941 tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat tacctgtctg
     3001 gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc gaggttgatc
     3061 tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt ggggccagaa
     3121 cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta gttaacgtca
     3181 atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag acgtcacgtc
     3241 ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct gctggtggga
     3301 cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg tctatccagt
     3361 cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa cggtgagaat
     3421 aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg cgttattttg
     3481 ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt cagtatgcat
     3541 atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc attacaaaat
     3601 attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt aagcactgct
     3661 gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc ggacgtcctt
     3721 tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga cacaggcgga
     3781 agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc agcaagaaat
     3841 cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata
     3901 tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct
     3961 ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt
     4021 tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta
     4081 gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc
     4141 agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt
     4201 tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc accttttcag
     4261 gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca
     4321 gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga
     4381 gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac
     4441 ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt
     4501 atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc gctgctggtg
     4561 gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac
     4621 agagaatcga tatctatttt actgactaat accagtcagg aatcttggct tattaacagc
     4681 aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcactatta
     4741 ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg
     4801 agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc
     4861 agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt
     4921 aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta
     4981 cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta ctacattaac
     5041 ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt gccgcctaaa
     5101 agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc ctggcggacg
     5161 ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg aaacaggttc
     5221 tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga actcgcacat
     5281 aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc
     5341 gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac
     5401 gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg
     5461 ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag
     5521 acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg
     5581 tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg
     5641 aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg
     5701 taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat aaagatacgc
     5761 cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg
     5821 gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg ctgcaatatg
     5881 gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga
     5941 tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagacgaat
     6001 tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt gcaaccaata
     6061 ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa
     6121 gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca ctagaattaa
     6181 acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa
     6241 agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc
     6301 cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga
     6361 taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg cttatttaga
     6421 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
     6481 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
     6541 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
     6601 acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct tttgtgttga
     6661 tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
     6721 ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca aaccgcaaat
     6781 gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
     6841 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
     6901 cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
     6961 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg tgaatgttga
     7021 cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct tcggtacaca
     7081 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcgtgacttc
     7141 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
     7201 acgaattccc tgattttcta attgctccag caacacttgt tggttatttc cacgaaatgc
     7261 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
     7321 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
     7381 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     7441 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     7501 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     7561 aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg ctgacgcgcc
     7621 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     7681 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     7741 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     7801 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     7861 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     7921 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     7981 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     8041 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     8101 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     8161 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     8221 gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt tggccctggg
     8281 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct ctccatgtcc
     8341 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
     8401 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
     8461 aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
     8521 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
     8581 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
     8641 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
     8701 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcaca
     8761 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
     8821 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
     8881 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
     8941 gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg tggttccggt
     9001 acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt gcaccgtggg
     9061 cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga agtgaaagcg
     9121 gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt ttacggttcg
     9181 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
     9241 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
     9301 ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag cgaaccggcg
     9361 ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg tggctggaat
     9421 ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg tgcagacaaa
     9481 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
     9541 agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg cctgcgtaag
     9601 aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta ccgcctgtat
     9661 gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
     9721 caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg tctgttcgat
     9781 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
     9841 cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg cgagtttctt
     9901 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct cgatactggt
     9961 ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa aggcaaagat
    10021 ttcctcaacc tgttttctta taccggcagc gctaccgtgc atgcgggatt aggcggtgca
    10081 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga acgcaacctg
    10141 cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
    10201 ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac cttctctaac
    10261 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat
    10321 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa acgtggcttc
    10381 cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
    10441 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
    10501 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
    10561 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
    10621 tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
    10681 tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg caacaggatc
    10741 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
    10801 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
    10861 aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac ctgtggcagc
    10921 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac gttgcgctgt
    10981 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc
    11041 cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc atcgactggc
    11101 tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
    11161 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg gtgacctatc
    11221 cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg gaagaactac
    11281 aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc caggggatca
    11341 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg cgtcgcgaac
    11401 gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
    11461 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt aagcaactgg
    11521 tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
    11581 ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg gacagcgggc
    11641 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
    11701 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaatg
    11761 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
    11821 tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
    11881 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
    11941 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
    12001 acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa ggcggcggta
    12061 aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa caatatgtgg
    12121 cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa gcagaaactg
    12181 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
    12241 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg gcggatgctt
    12301 ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg gctgctgcag
    12361 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
    12421 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
    12481 aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc
    12541 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
    12601 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
    12661 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt
    12721 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
    12781 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
    12841 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
    12901 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
    12961 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gcgggtgtgc
    13021 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
    13081 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
    13141 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
    13201 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
    13261 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc ctgcagtgga
    13321 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
    13381 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
    13441 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
    13501 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
    13561 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
    13621 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
    13681 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
    13741 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
    13801 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
    13861 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
    13921 cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
    13981 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
    14041 ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa
    14101 ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg cgaagggatt
    14161 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
    14221 agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc tgatgcgaaa
    14281 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
    14341 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc
    14401 aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
    14461 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat gcgtgtggag
    14521 atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc ggaaggtctg
    14581 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
    14641 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
    14701 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
    14761 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
    14821 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
    14881 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
    14941 ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc
    15001 ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg cttggcgcaa
    15061 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
    15121 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
    15181 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt gccgacggat
    15241 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
    15301 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
    15361 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
    15421 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
    15481 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
    15541 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
    15601 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
    15661 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg cgcaacaccc
    15721 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
    15781 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
    15841 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
    15901 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg ctggccggtg
    15961 tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct taattataaa
    16021 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
    16081 gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt gtagctcaca
    16141 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
    16201 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
    16261 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
    16321 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg agtcaaaggt
    16381 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    16441 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    16501 gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    16561 atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag acgacgatta
    16621 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    16681 gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg
    16741 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    16801 tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    16861 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtctatcatc
    16921 agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gtgaccagag
    16981 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    17041 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    17101 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    17161 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    17221 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    17281 atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaacgttag
    17341 cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa atagcaaaga
    17401 agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac tgatcgactg
    17461 aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt atcagggcgc
    17521 agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc tcaaatgtca
    17581 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcctgca
    17641 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    17701 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    17761 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    17821 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    17881 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    17941 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    18001 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caagcatccg
    18061 ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggc
    18121 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    18181 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    18241 tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc gactcatcaa
    18301 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    18361 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    18421 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    18481 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    18541 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc accagttggc
    18601 ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac tcagactctt
    18661 ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    18721 caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    18781 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    18841 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    18901 cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt taaagctcac
    18961 aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa gagttacacg
    19021 gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat
    19081 ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac atagaagcca
    19141 gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc gtgctggtaa
    19201 atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag cagaccattc
    19261 gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg
    19321 atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc ggtaaaacgc
    19381 tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa tacgcgaata
    19441 aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa ctgacgaaag
    19501 tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt ctaccagacg
    19561 ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc gatctctacg
    19621 cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg
    19681 ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc tgccgggata
    19741 cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag accctggttg
    19801 taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc gtctttcgga
    19861 tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc cggtttcagg
    19921 gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt ggtctgtact
    19981 tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa acggtaggaa
    20041 acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc gtcaccgatg
    20101 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    20161 ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc gccaccaggt
    20221 acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccaga
    20281 tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc tttgtatgcg
    20341 ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaaa
    20401 ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg gttttcgtgg
    20461 gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc cagtttagca
    20521 ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt
    20581 gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata
    20641 cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa
    20701 tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    20761 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    20821 aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg gaaaccgctt
    20881 cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg gcgacaaaaa
    20941 aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa tttttagccc
    21001 ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa accccatagc
    21061 gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca aatcatctgt
    21121 caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca ttgactccac
    21181 tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta gcccagatgc
    21241 ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc agcgcgattg
    21301 ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg gctgatcttc
    21361 gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga cacgcgcaat
    21421 tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag tgtacataat
    21481 caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac aacaaagatc
    21541 aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa aacataaatt
    21601 aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg ttaatgtgaa
    21661 tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc tataagcgga
    21721 tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga tcattgtttg
    21781 gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat ggtgagttgt
    21841 atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct gtctggctga
    21901 catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat gaaagtctat
    21961 ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca gcgctgaaag
    22021 agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg tcttttcatc
    22081 tggaagcgat tctcggggag gtgggtagac tggccccctg aatctccaga caaccag
//
LOCUS       DAJVPT010000066        21971 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVPT010000066 DAJVPT010000000
VERSION     DAJVPT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            229..855
                     /locus_tag="OQM47_002467"
     CDS             229..855
                     /locus_tag="OQM47_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1480584.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            876..2977
                     /locus_tag="OQM47_002468"
                     /pseudo
     CDS             876..2977
                     /locus_tag="OQM47_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            2990..3628
                     /locus_tag="OQM47_002469"
     CDS             2990..3628
                     /locus_tag="OQM47_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1480585.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3692..4360
                     /locus_tag="OQM47_002470"
     CDS             3692..4360
                     /locus_tag="OQM47_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1480586.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4357..5142
                     /locus_tag="OQM47_002471"
     CDS             4357..5142
                     /locus_tag="OQM47_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1480587.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5146..5958
                     /locus_tag="OQM47_002472"
     CDS             5146..5958
                     /locus_tag="OQM47_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480588.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5970..7392)
                     /locus_tag="OQM47_002473"
                     /pseudo
     CDS             complement(5970..7392)
                     /locus_tag="OQM47_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7518..7823)
                     /locus_tag="OQM47_002474"
     CDS             complement(7518..7823)
                     /locus_tag="OQM47_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1480589.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(7931..8007)
                     /locus_tag="OQM47_002475"
     tRNA            complement(7931..8007)
                     /locus_tag="OQM47_002475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7971..7973),aa:Val,seq:gac)
     gene            complement(8012..8088)
                     /locus_tag="OQM47_002476"
     tRNA            complement(8012..8088)
                     /locus_tag="OQM47_002476"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8052..8054),aa:Val,seq:gac)
     gene            8396..9652
                     /locus_tag="OQM47_002477"
     CDS             8396..9652
                     /locus_tag="OQM47_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1480590.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9693..11066)
                     /gene="mdtK"
                     /locus_tag="OQM47_002478"
     CDS             complement(9693..11066)
                     /gene="mdtK"
                     /locus_tag="OQM47_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1480591.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11281..11922
                     /gene="ribE"
                     /locus_tag="OQM47_002479"
     CDS             11281..11922
                     /gene="ribE"
                     /locus_tag="OQM47_002479"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1480592.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(11962..13110)
                     /gene="cfa"
                     /locus_tag="OQM47_002480"
     CDS             complement(11962..13110)
                     /gene="cfa"
                     /locus_tag="OQM47_002480"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1480593.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13401..14612)
                     /locus_tag="OQM47_002481"
     CDS             complement(13401..14612)
                     /locus_tag="OQM47_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1480594.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            14725..15657
                     /locus_tag="OQM47_002482"
     CDS             14725..15657
                     /locus_tag="OQM47_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1480595.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15654..16679)
                     /gene="purR"
                     /locus_tag="OQM47_002483"
     CDS             complement(15654..16679)
                     /gene="purR"
                     /locus_tag="OQM47_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1480596.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            16978..17067
                     /gene="ynhF"
                     /locus_tag="OQM47_002484"
     CDS             16978..17067
                     /gene="ynhF"
                     /locus_tag="OQM47_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1480597.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17233..18402
                     /locus_tag="OQM47_002485"
     CDS             17233..18402
                     /locus_tag="OQM47_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480598.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18548..19129)
                     /gene="sodB"
                     /locus_tag="OQM47_002486"
     CDS             complement(18548..19129)
                     /gene="sodB"
                     /locus_tag="OQM47_002486"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1480599.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19257..20084)
                     /gene="mepH"
                     /locus_tag="OQM47_002487"
     CDS             complement(19257..20084)
                     /gene="mepH"
                     /locus_tag="OQM47_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1480600.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20419..20766
                     /gene="grxD"
                     /locus_tag="OQM47_002488"
     CDS             20419..20766
                     /gene="grxD"
                     /locus_tag="OQM47_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1480601.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20805..21305)
                     /gene="tnpA"
                     /locus_tag="OQM47_002489"
     CDS             complement(20805..21305)
                     /gene="tnpA"
                     /locus_tag="OQM47_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1480602.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21313..>21971
                     /locus_tag="OQM47_002490"
     CDS             21313..>21971
                     /locus_tag="OQM47_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1480603.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5664 a         5143 c         5706 g         5458 t
ORIGIN      
        1 tttgtaattt taattgataa cgatcaatgt taagggctca gaataatcac attatttttt
       61 ctcctaacca taaaggatta gtttatcggc gttaaatctt ttctcataca ctatcgggtt
      121 agggaaaata tctggtgttg gggacagagt cagcagcgta ttagttgaat gcttaatatc
      181 aggctgctga attgtcagca ctgtgtttgc gaagaggatg cgaaataaat gaacccggtt
      241 gatcgtccac tattagatat tggcttaacg cgattagaat tcttgcgtat atcaggaaaa
      301 ggccttgctg gtctaacaat tgcccctgcg ttactgtcac ttttgggttg caaacaggaa
      361 gatattgata gcggcacagt agggttgata aatacaccca aaggggtgct ggtgacccag
      421 cgagcgcgat gcacgggttg ccaccgctgt gaaatctctt gtaccaactt caacgatggc
      481 tcagtaggga cattcttctc ccgtatcaaa atccatcgca attatttctt tggcgacaac
      541 ggggttggct ctggcggcgg cctgtatggc gatctcaact ataccgcgga cacctgccgt
      601 caatgcaaag agccgcaatg catgaacgtc tgcccgattg gtgcgattac ctggcagcag
      661 aaagaaggct gtattaccgt cgatcataaa cgttgtattg gctgtagcgc ctgtactacg
      721 gcatgcccgt ggatgatggc caccgtaaat accgaaagta aaaaatcctc gaaatgtgtg
      781 ttatgcggtg aatgcgcaaa cgcctgccca acaggggcgt taaaaattat cgagtggaaa
      841 gatattactg tgtgaatctt gcaaaggaaa aaatcatggc taacggttgg acaggtaata
      901 tattaagagt caatctcacg acaggaaata ttaccctcga agattccagt aagtttaaaa
      961 gttttgtcgg tggcatgggc ttcggctaca aaattatgta tgacgaagta ccgctaggca
     1021 cgaaaccttt cgatgaagcg aataaattag tctttgctac cggcccatta cctggatctg
     1081 gtgccccctg tagttctcgc gtaaatatca cctcactttc tacttttacc aaaggaaatt
     1141 tagtcgcgat gcccatatgg gtggcttttt tgcagcgcaa atgaaattcg ctggatacga
     1201 cgtcattatt atcgaaggga aggcgaaatc accggtatgg ctgaagatta aagatgacaa
     1261 agttagcctg gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa cgacggaaga
     1321 aatttgtcga ttgaccagcc cggaaacctg tgtggcggct attggtcagg ctggggaaaa
     1381 ccttattcct ctctctggca tgttgaatag ccgtaaccac agcggcggtg cgggaactgg
     1441 cgcaataatg ggttcgaaaa acctgaaagc gattgcggtt gaagggacga aaggggtcaa
     1501 cattgccgat cgtcaggaga tgaagcgtct caatgattac atgatgactg aacttattgg
     1561 tgcgaataac aaccatgtcg tgccaagtac gccacaatcg tgggcagagt attcagatcc
     1621 caagtcacgc tggacagcac gtaaagggct gttttggggc gcggctgaag gtggtccgat
     1681 tgaaacgggt gaaattccgc caggcaatca gaatacggtc ggctttcgta cctataaatc
     1741 cgtttttgac ttaggaccgg cggcagagaa atacacagta aaaatgagcg gctgccactc
     1801 ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt ttggcgtgcc
     1861 cagcacaggt ggtaacactt gtgtagcaaa ctttgtccat accaccatct tcccgaacgg
     1921 gccgaaagat tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg ttggtctgaa
     1981 tttgttcgat gactacggcc tatggtgtaa ctacgggcag ttgcatcgcg actttactta
     2041 ttgttacagc aaaggtgtgt tcaagcgtgt tctgccagct gaagagtatg cagaaattcg
     2101 ctgggatcaa ctggaagcgg gtgacgttaa cttcattaaa gatttttact accgtctggc
     2161 gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tatgccatcg cagaacgctg
     2221 gaatttgggt aaagagtact ggggctacgc gaaaaataaa ctctggtcgc cgtttggcta
     2281 tccggttcac catgccaatg aagcgtcagc gcaggtcggt tccattgtta agtgtatgtt
     2341 caaccgtgac tgcatgacgc atacccatat caactttatt ggttccggct tgccattgaa
     2401 actgcaacgt gaagtggcga aagaactttt tggttctgaa gatgcttacg atgaaaccaa
     2461 aaactacacg ccaatcaatg acgcaaaaat taaatatgca aagtggtcgc tgttgcgggt
     2521 ctgtttgcat aacgccgtca ctctgtgcaa ctgggtctgg ccaatgaccg tttcgccgct
     2581 gaaaagccgt aattaccggg gcgatctggc gcttgaagcc aaattcttca aagcgatcac
     2641 cggcgaagag atgactcagg aaaaattaga tttagctgca gagcgtatct ttacgttgca
     2701 tcgtgcctac acggtaaaac cgatgcaaac caaagatatg cgtaatgaac acgatcttat
     2761 ctgttcctgg gttttcgaca aggatccgca gatcccggtc tttactgaag gtactgacaa
     2821 aatggatcgt gacgatatgc atgcttcgct cacgatgttc tacaaagaaa tgggctggga
     2881 cccacagctt ggttgtccaa cccgcgaaac attgcagcgt ctggggctgg aagatattgc
     2941 cgccgatctg gcagcacaca atctactgcc tgcgtaagga atgggtaaga tgaaccatca
     3001 ggatgaattg ccgttagcga aagtcagcga agttgacgag gcgaagcggc aatggttgca
     3061 ggggatgcgt caccccgttg atacggtaac cgaaccagaa cctgctgaaa tcctggctga
     3121 gttcattcgc cagcattctg cggctgggca gctggtggcg cgcaccgtgt ttctctcgcc
     3181 accctattcg gttgcagaag aggaactgtc agttttgctg gaaagcatta agcagaacgg
     3241 agactacgcg gatatcgctt gtatgacggg aagtcaggat gattactatt actcaacgca
     3301 ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg acatttgtcg
     3361 cgccattgca cacgccgttc gttttgagtg ccagacgtat cctcgtcctt acaaagtcgc
     3421 catgctgatg caagcgccat attacttcca ggaagcgcag attgaagccg cgatcgccgc
     3481 gatggatgtt gcacctgaat atgccgatat acggcaggtg gagtcatcga cggcggtgtt
     3541 gtacttgttt agcgagcgat ttatgaccta tggcaaggct tatggcttat gcgaatggtt
     3601 cgaggtagag cagtttcaga acccctgaaa tttcagggag atgtcatgga tagggtggac
     3661 agttgcagcc actgcattag gggataatca gatgtccttc actcgacgca aatttgttct
     3721 ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag cgaacacgag
     3781 gcaagaaaag gaagttcggt atgccatgat tcatgacgag tcacggtgca atggctgtaa
     3841 tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa gccgtttatc
     3901 gatagcacac attccagtca ccgataacga caacgaaacg cagtatcact tttttcgcca
     3961 gtcatgtcag cactgcgaag atgctccgtg catcgacgta tgcccgacgg gggcatcgtg
     4021 gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag tgcatcggct gcagttattg
     4081 cattggcgct tgtccgtacc tggttcgcta ccttaaccct gtcactaaag tggcagataa
     4141 atgtgatttc tgcgctgagt cccgactggc aaaaggcttt ccgcccattt gcgtcagcgc
     4201 ctgtccggaa cacgcattga tctttggtcg cgaagatagc ccagaaattc aggcgtggtt
     4261 gcaggataat aaatactatc aatatcagct acctggtgcc ggaaaaccgc atctgtatcg
     4321 ccggttcggt caacatttga ttaaaaagga aaatgtatga acccgtcgca acatgctgaa
     4381 cagtttcaga gccagttagc gaactatgtg ccacagttca ctcccgaatt ttggccggtg
     4441 tggttgatca ttgccggagt attgctggtt gggatgtggt tggtgctggg gctgcacgcc
     4501 ttgcttcgtg ctcgtggcgt gaagaaatca gccaccgatc atggtgagaa ggtttatctt
     4561 tacagcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt attgttgctg
     4621 gcaagtgggc tgataaatca ctttgcgatg gtgggcgcaa ctgcggttaa aagtctggtt
     4681 gcggtgcatg aagtttgcgg atttttgtta ctggcatgct ggctcggctt tgtgctgatc
     4741 aatgccgttg gggataatgg tcaccactat cgcattcgtc gtcaggggtg gctggaacga
     4801 gcggcaaaac aaacgcgatt ttatttgttt ggcattatgc agggggaaga acatcctttc
     4861 ccggcaacaa cacagtctaa atttaatccc ttacagcagg tcgcctatgt tggtgtcatg
     4921 tatggattgc tgccgttgtt actattgacg gggctgttgt gtctctatcc gcaagccgtg
     4981 ggagatgtgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc tctggcattt
     5041 ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg tacgccacac
     5101 gaaaccttta aaagcatggt cgatggctat caccggcact aacgaatgat cattacacga
     5161 gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggttcct gcgccatttc
     5221 ccacgcggcg gtagttatgc cgatattcgt catgcactga ttgaggaagg atatacggac
     5281 tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga aaacttcgcg
     5341 catcaggaag tttcctctat gcaaaaactg gccacagacc ccggagagat accattttgc
     5401 agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga taacgcccga
     5461 atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt gcggattgga
     5521 agtgtcggct ttaacagtca cattggcagc tccggtgaac gcgcacgggt tgctgtaacc
     5581 ggtaattctt cgcggataag cagcgctgga gatagtagtc ggattgcgaa taccggaatg
     5641 cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg tgacctggtg
     5701 caaatcgcca gctttggcgc aaacgccaga attgccaaca gcggcgacaa tgtacacatc
     5761 attgccagcg gtgaaaattc aacggtagtg agcacgggcg tggtcgactc aattattctc
     5821 gggctagggg gaagcgccgc actggcatat catgacggcg aaagagttcg cttcgccgtc
     5881 gccattgaag gtgaaaacaa tattcgaacc ggagtccgct atcgtcttaa tgaacagcat
     5941 cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgccg acgagcacgg
     6001 gatgcaggct ttacgaccgc ccgagccatc gcctcaccaa tttctgcaca tgccgtaagt
     6061 ccctgaacga aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc tacgcgtcca
     6121 cgaatatctt cgggttgctg taacgtataa tcttcgccaa catcagggat ttcagatccc
     6181 cgcttcacgt aaggccagta gtcgacgaat agactctgaa gggattgccg cataattatc
     6241 gatgaaaacc ctggcaaggc ctttactgaa ccgtttatgg tcgtgttcat tcagatgagg
     6301 aacaatttcc tgtacggctt catgcaggcg cagaatgaca taacgccagg gaacagtatg
     6361 tttttctcgt ttattgcgtt cgacttcctg gagatttttt tctgcccagt caaatgggtc
     6421 gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa tcgacattca ggctctctaa
     6481 ggctatgcgt tggctccagt ctggatcagc aaacttgatt tcctctacta tcaatctaaa
     6541 tacccgatcc aaaaggccct cttcaccttt ttgaatctca gcatttaatg cctgatcggt
     6601 gacgatgtgt aagggctcgt agggaatagg gcaatagaaa tcggcttcgg gtaaaatacc
     6661 cgtgcgcgac atcaacgtaa tatttagctt ttcacttgcg ttatcgcggt gaaaaacgac
     6721 gtgttgttta tcatcttcaa tgaacgaacc atgctgaata gccactgcca ttgccgcatc
     6781 cagtccgctc aaggatgttc ccataatacc cacgttacac gcatcgacct ttgcttccat
     6841 caggcctgac cacgggctgg gaaaatacgt tctggttgct tcttcttcat caggccagac
     6901 gtgacccgtg gcaatcaccg ctaaatcaaa cgtctcgctg ggtaaatcct gatttgtagc
     6961 gagcatgacg ccagcatttg taatttgcag atcggtaacc tggcatgatt cataaacagc
     7021 cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt aaaaattgat cgcggaaata
     7081 ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc aaggtttctt ttttaacgcc
     7141 ataacgctgg agatggctgg cttcttgctt ttgtagccat tcgagatacg tacaattaat
     7201 cggcggtatt tcaatactgg caatatttgc cagcatcatt tttgagtttt cctcatcact
     7261 gtatggcatc ccgacaccgg cctcgtcagc ctgctcgaag atagaaatag aaagtggagt
     7321 ttgttgctgt agaagcgaga ataaggtgta gatccccgta ggcccggcac ccacaatagc
     7381 aatttttttc attgttactt acctgtacgc attctttcga atttagtgtt atcagataag
     7441 tttggttgag atttttagaa tttgagaaat ttcagacagg gatgaaaagg gcgctaaccg
     7501 cccttgatta aattctgtca ggcgagtttt gcctgattga tttgactaag tggctcattg
     7561 acatcgaaaa ctttggcgac aacttcctca acgccgagat ttttcagtcg ggcagtatgt
     7621 ttttccagat aggcaagggc gcttttttca tcagtgaaaa ggtagatccc accggcttcg
     7681 tggttctttt cactttctgt ccataccttc cacagaaaac caggttcctg attaatcgac
     7741 tcagcaagtg gtttaagctg ctcagccatt gcgtcgccaa atgggccatt aaaagcaaaa
     7801 tgaagttgta acaaagttgc catagcgatt ctccttctga taacgcatac gaagacagtg
     7861 taaatctttt aacgtattgg atacagagaa gaaacagagg gaaaaaggaa aagaccagat
     7921 taagaaaatc tggtgcgtcc gagtggactc gaaccaccga cccccaccat gtcaaggtgg
     7981 tgctctaacc aactgagcta cggacgcagg atggtgcgtt caattggact cgaaccaacg
     8041 acccccacca tgtcaaggtg gtgctctaac caactgagct atgaacgcaa cgttgtaggt
     8101 gacaacgggg acgaatatta gcggcagagt ggaaaggtgg caagaggcaa aacgtaattt
     8161 tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat tggctatatt ttgttgattt
     8221 gcaagggcga tttttattca ggatcgcatt tacatctgat acaacccaat aattcgtgta
     8281 tcagagttta aacagagttg tttgccacca tctcaaatct gtcgtggata aatcctccgt
     8341 agcctttaca cttaggtagc aataaaggca cacagatgca gcaaaggaat aaatcatggg
     8401 atctgatgcg aaaaacttga tgagcgacgg gaatgtgcaa attgttaaga ccggcgaggt
     8461 ccttggcgcg acgcaactta ctgaaggcga gttgattgtt gaagctggcg gaagagccga
     8521 aaataccgtg gtcacggggg ctggctggtt gaaagtggca accggtggga tcgccaaatg
     8581 cacacagtac ggtaacaatg gcacgctatc ggtcagcgac ggtgccattg ccacagatat
     8641 tgttcagtcc gagggaggcg caattagtct ctctacgctc gctacggtta atggccgcca
     8701 tcccgaaggt gaattcagcg ttgatcaggg ttatgcctgc ggtttgttgc tggaaaatgg
     8761 cggtaacctg cgtgtactgg aaggacatcg cgcggaaaaa atcattctcg atcaagaggg
     8821 cggcctgttg gttaatggga caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt
     8881 ggtgtatcca agtggagaag ccagcaattg tgagattaat cagggcggcg tttttatgct
     8941 ggccgggaaa gccagtgata cgttgcttgc tggtggcacc atgaataatc tcggtggtga
     9001 agactctgac actattgttg agaatggatc catttatcgt ctggggacgg atggccttca
     9061 gctctacagt tccggtaaga cgcaaaacct gtcggtgaat gtgggggggc gggctgaagt
     9121 gcatgccggt acgctggaaa atgcggtaat acaaggtgga acagtgatcc tgttgtcacc
     9181 caccagcgcg gacgaaaatt ttgtcgtaga ggaagatcgc gcaccggttg aactgaccgg
     9241 gagtgttgca ttactggacg gcgcttcaat gattattggt tatggcgcag agctgcaaca
     9301 atcaacgatt actgtacagc agggcggtgt gttgattctc gacggcagta cggtaaaagg
     9361 tgacagcgtc actttcattg ttggtaacat caatctgaat ggcggaaaac tgtggctgat
     9421 cactggtgcg gcaacgcatg tgcaactgaa agtgaaacgc ctgcgcggag agggagcgat
     9481 ttgcctgcaa accagtgcga aagaaatttc acctgacttc atcaatgtga aaggggaagt
     9541 taacggggat atacgcgttg agataacaga tgccagtcgg caaactctgt gtaacgctct
     9601 gaaattacag ccagacgaag acgggattgg cgcaacgctc cagcctgcgt aaaaacacag
     9661 gcgcaacccg tgaaggttgc gccttgtctt tattagcggg acgctcgttg cagaatgatg
     9721 gctgacggca tacgttgcag gaaccgcata cgcagcatca tcataatggc tgccgatgtc
     9781 aggccgataa taaagcctat ccagaagcct gctggcccca taggttcaac gaccagatcg
     9841 gtcagtgcca gaatatagcc gcttggcaag cccagcaccc agtaagctgt aaaggtgata
     9901 tagaaaatgg aacgcgtatc tttataacca cgcaaaatcc cactgccaat cacctggatt
     9961 gagtcagaaa tctgatatac cgccgccagc aacatcaaat gcgcagccag cgttacaacc
    10021 tcgggattgt cgttgtacaa cagggcgatt tgctcccgca gtgaaaccgt gaaaatggcc
    10081 gtcagggttg ccatacagac acccaccata agcccggtcc gcgcagcggt ttgcgcatcc
    10141 agcgttgagc cctgacccag acgataacct acgcggatag ttaccgctgc cgccagcgac
    10201 attggaagca caaacatcag tgaactaaag ttcagggcaa tctggtgtcc tgcgacatca
    10261 acaataccga gcggagacac taacagagcc acgacggcaa acagtgtcac ttcaaagaac
    10321 agtgccagcg caatcggcaa accgagttga atcagtcgtt tcataaccgc aggctcgggc
    10381 ttagcggtgc ctttttcgtt acgaatatcg cgcatggagc gggcgcgttt aatgtaagaa
    10441 accatggcaa ggaacatgac ccaatacacc gccgcagtag ccacgccaca accaacgcca
    10501 ccgagctcag gcataccgaa atgaccataa ataaagatat agttcaccgg gatgttcacc
    10561 agcaggccga taaagcccat taccataccc ggcttggttt ttgccagacc ttcacattgg
    10621 ttacgggcaa cctggaagaa cagatatccc ggcgcgcccc acaacaacgc acgcagataa
    10681 cccacggctt tgtccgccag agccggatcg atgttttcca tgtagcggat aatgtaacct
    10741 gcattccaca gcaccagcat aatgagaacg gaaacaaaac ctgccagcca gaaaccttgt
    10801 cgcacctgat gcgcaatgcg ctcacgtcga ccggaaccat ttaattgcgc gataaccggc
    10861 gttaatgcca gcagcagtcc gtgaccaaag aggatcgccg gaagccagat agaagtaccg
    10921 atagcgaccg ccgccatgtc ggtggcacta tagccgcccg ccatcacggt atcgacaaaa
    10981 cccatcgcag tttgggcgat ttgcgcgaga atcaccggga ttgctaatgc taataacaga
    11041 cgcgcttcac tgatatactt ctgcacgtga acacctttta tttgtagtta tatgagagac
    11101 taaaaagccg cctgaatggg cagcaaaaag aagaagcagg ggaaattcag tctattgtag
    11161 cgaggattta ttaattctcc agtgaaaaaa tagccattct taccccttca ctggtaaggg
    11221 cgttttccac ctgctattgt gctgaacaga atgtcttaac tgatttcagg agttgtaagt
    11281 atgtttacgg ggattgtaca gggcaccgca aaactggtgt cgattgacga gaaaccaaat
    11341 tttcgtacgc atgtggtgga gttacccgac cacatgctgg acggcctgga aaccggtgct
    11401 tccgtggcgc ataacggttg ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt
    11461 tttgacctga tgaaagaaac gttacgcatt accaatcttg gcgatttaaa agtgggggat
    11521 tgggtaaacg ttgagcgtgc ggcgaaattc agtgatgaaa ttggcggaca cttaatgtca
    11581 ggtcatatta tgaccaccgc tgaagtggcg aaaatattaa cctcggaaaa taatcgccag
    11641 atctggttta aagtccagga tagtcagttg atgaaatata tcctgtacaa aggatttatt
    11701 ggcatcgacg gtattagcct gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat
    11761 ttaattccgg aaacactgga acgcacgact cttgggaaga aaaaacttgg cgcacgcgtc
    11821 aacattgaaa tcgatccaca aactcaggca gtggtagata cggtagaacg tgtgctggcg
    11881 gcacgagaaa atgccatgaa tcaaccaggt acagaagcct gatctgaact cgcccccgaa
    11941 gagggggcga tagaatagcc tttagcgagc gactcgaagg ccgttttcaa caccgcgtga
    12001 gaacaccacc tgccagagct gaatatcacg ggcgcggaaa gcacctgcgc aggcattcag
    12061 ataataggta aacatccgtt taaagcgttc actgtagtta tccgcaattt ctggccatgc
    12121 ggcgaggaat cgttcatacc acgccatcaa cgtagtatcg taatcagcac cgaagttatg
    12181 ccagtcttcc atcacaaagt ggggttcgct ggattgagca atctggcgta cagagggcag
    12241 gcaaccgttc ggaaaaatat atttattaat ccagggatca acattcagat cggttttttt
    12301 cgaaccgata gtatggagca ggaatatacc ttccggtttc aaattacgat ccaccaccgc
    12361 aaaataggta tcgtaatttt tcggtccgac gtgctcgaac atccccacag aaacaatacg
    12421 atcaaactgg tcgttcaggt cacgataatc ttgcagcaaa atggtgacat ccaggccttt
    12481 acagcgttcc tgagccattt tttgctgttc ggcagaaatg gtgacgccga ccacgcttac
    12541 gtcatagtta gatgccatgt agtgcgctag tccgccccag ccgcagccaa tatccagtac
    12601 gcgcatccct ggttttaact gcaatttttc acaaatcatt ttgagcttcg cctgctgggc
    12661 agattccaga ttatcggcat ctttccagta agcgcaggaa tattgcatga agggatcaag
    12721 catgcggctg aacaagtcat tacccaaatc gtaatgctcc ttgccgacta tccaggcacg
    12781 ttttttactc tgcagattga agagacgagc gccggcaata cgcagcgtgt ctttgaaatg
    12841 atgggggagt tggttctcga gacctgcgcg taagactttg ctaaaaaaca tatccagtcg
    12901 gtcacattcc caccagccat ccatataact ttcgcctaac cccaaagagc cttcctgcag
    12961 aacgcgttta aaaaaatcgg ggtttttcac acgaatatcc gccggggcag aaccgttaat
    13021 ggctataccg gcacggctaa gtaattcgtt ggcgatacgg taccagttgt catccggtac
    13081 actgacttct tctatacacg atgaactcat agtttctcca tcactggtcg gtgatcagaa
    13141 ccttaaaaca gcgtagacgc tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga
    13201 gtctataatc cgacgtagaa cagagggaaa ggaggaaccc ttgccgaaat gaccatccat
    13261 agtgagtcgg gaacgatcct gtccccgtaa aaacataata tttatcgtag ctttaacaac
    13321 tcgaaattag tataggccgc taatttcaat catacaacaa aattttgtta gcagcctaag
    13381 tataagtata tcgatataga tcagtgtgat tcgctatgag cgacttcggc attgccatga
    13441 ttcgggcagt caacttcttc acaacgttgc atcatgtaac ccagcgcgac cagcactact
    13501 gttgataaca tcacgctggt ggtggtgagc aatggcgtgc tgatactaat aagccaggaa
    13561 actaccagac ttgcgaggaa gcacagaccc agttgcagag tgttctgcaa cgctgcggcg
    13621 cgaccggttg catgtgggaa gggacgcagc gcctgggcga caacaatagg gtagatcgcg
    13681 ccattggcaa tcgccatcac acagaatggg atcaggattt cgaccagcga cacatggcta
    13741 atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa acagcaccag caaccacggt
    13801 aataactgct tgccttgcca tttctgcagt gcggcgcgac agccataacc accaatcaga
    13861 aacgcgatag tttgcgggac ataacttaaa ccaataactg ccgggctgta gcccatttca
    13921 ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac tggctgaaca ggctgcgtat
    13981 atcagcacgt tgccgcgata ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc
    14041 tgactattgt tacgggcctt cgtcgtgggt ttgagccaga aaataggcag aatcagcacc
    14101 acggtaatgg caaacagggt ggcgaaaatc gcctgccagg aaaaatggac cagcagccag
    14161 cttcctaaca ggggagccag tgccggagac agacccacca gcggcatgat ggtcgcaaaa
    14221 atacggttga ctttctgtga aggataataa tctgtcacta atgcttgcca gataaccgcc
    14281 gcggcgcaga cccccacagc ctgtacaaaa cgcaatacca gcagcgtggc ggcgttttct
    14341 acccacagca tccccagact acctaacgca aaaattgtca ggccgattaa taacaccggt
    14401 ttacgaccat aacggtcgga gagtggcccc cacagaagct gggctgcggc aaaaccggca
    14461 aggaacagac taaggctggc actgacagca gacgcaggcg tttgcaggtc ggcctgtatg
    14521 gcggcgaaag caggcagata catatcggtt gccagaaaac cgagtacgct caaacccgcc
    14581 agccagacta aaaatctttt cccaggttgc atgtatattc tctaaatctc tcaattaaac
    14641 accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa cggtaatatt tgatgactgg
    14701 tttcaaaaat tttgcaggca gactatgtgg tcagaatatt cactcgaagt tgttgatgcg
    14761 gtagcgcgta atggtagttt tagcgctgcg gcacaggagc tgcatcgtgt tccttctgcg
    14821 gtcagctata ccgtgcgtca gctggaagag tggctggcgg taccgctctt tgaacgacgg
    14881 caccgtgatg tggagctgac cgctgctgga gcgtggtttc tcaaagaagg gcgctctgtt
    14941 gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga tagcgaacgg ctggcgcggt
    15001 cagttagcta tcgcagtgga taatattgtc aggccagaac gtacacggca gatgatcgtt
    15061 gatttttatc gccattttga tgatgtcgaa cttcttgtct ttcaggaagt gttcaacggt
    15121 gtctgggatg cgctttccga cgggcgcgtg gaactggcaa ttggcgcaac acgcgcgatt
    15181 ccggtaggcg gtcgttatgc cttccgggat atggggatgc taagctggag ttgcgttgtt
    15241 gccagtcacc acccgctggc gttgatggat ggcccgttca gcgatgatac gttgcgcaac
    15301 tggccgtcgt tggtgcgcga agacacctcg cgaacgctac ctaaacgtat tacctggttg
    15361 ctggataacc aaaaaagact tgtcgtacct gactgggaat catcggcaac ctgtatctcg
    15421 gcaggattat gcatagggat ggtgccaaca catttcgcca aaccgtggct taatgagggg
    15481 aagtgggtag cacttgaact ggaaaacccc ttcccggatt cggcatgttg tctgacatgg
    15541 cagcaaaatg atatgtcgcc tgcgttaacc tggctgcttg agtatctcag cgatagtgag
    15601 acgctgaata aggagtggct gcgggagccg gaagagactc ccgcaacggg tgattaacga
    15661 cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt caatcaaacg cggatgcact
    15721 tcaatggact gcggttcttc acgtttgttg acgatacgat ccaacagcat gttgaacgct
    15781 gtttcaccca gcgaatcttt tggctgatgg atcgtggtca gcgccggcgt aaaatagcgc
    15841 gcgttgcgca cgttatcata accgatcagc gaaacatcct gcgggacgcg caggcccatt
    15901 tcatcagcag cacaaagtgc gcccattgcc atgatatcgc caccacagaa gacggcagta
    15961 gggcgatgcg gctgcgacag gatttgctgc atggcgcgat aaccggattc aggttcaaag
    16021 tcaccctgca caatccagct ttccggcacc ttgatcatcg cttcttccat cgccttcata
    16081 aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg ggccggggat gacgccgatt
    16141 tcgcggtgac cgcgttcaat cagataacgc ccggccatgt agccgccttc gaacgcgtta
    16201 tcaatgaccg catcggtgaa gtcagctttt gcttcacccc agtccatcac caccattggg
    16261 atatggcgat actcttccag catcgccagc aacggctctg ggtactcaga acacatcacc
    16321 agcagaccat cgacgcgttt ttgcgccatc atcgacagat aagcccgctg tttctcaaga
    16381 ttgttccacg cattacccag aatcagggtg taacctttct ggaagcaatt tttttcaact
    16441 gcttcaatga tctcggcaaa ataggccgct tcgctgctgg tcgccagcaa accgatagac
    16501 ttggtgtggt taaccttcag gctacgcgcc accgcgctag gggagtagtg taattcttta
    16561 atcgctgccc acacggcgtt gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg
    16621 tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta catcttttat tgttgccatt
    16681 ccatttcact ccagacccta acttcagctc ctgcaaaatc gcaaggtaaa cgtttgcctt
    16741 tacacacctt acgcacaaca aaagtgttgc ggtacgccgg aaaaacgaca cggagcgtca
    16801 ggaaggggtc aatggccagt acgcaaatag tgtaagcgtg gaattttggc cgatcttgac
    16861 gaaaagggga agtggtaaaa acacttatca ttataaatga cgggagattt tttcatcaca
    16921 gtgtgtaaaa atgcgtaata tcactttttg tgtggtaata aaaaaggaga aaacttgatg
    16981 agcaccgacc ttaaattttc actggtaaca acggttatcg tcctcggttt gatcgtagcc
    17041 gtgggtttga ctgccgcgct gcactgattt cctctcgagg gagcgcgttc tccctcgcat
    17101 ctccttcctg tttgttagca aaatggcaaa agtgttttct ctttttgtta ttttcatttt
    17161 tttgtgattg ctgtcatgct ttcgccactt taatgaatat cgcgtaatga cgcgaagagg
    17221 tggagataag gcatgaaaat taactatccg ttgctggcgc tggcgattgg cgcgtttggt
    17281 atcgggacaa cggagttctc gccaatgggc ttgttgcccg tcattgcgcg cggtgtggat
    17341 gtctcgattc ccgctgccgg aatgttaatc agtgcctatg cagttggcgt aatggtgggc
    17401 gcgccgctga tgacgcttct actttctcat cgtgcccgcc gcagtgcgtt gattttcctg
    17461 atggcaattt tcacgctagg caacgttctt tccgccatcg cgccggatta tatgaccctg
    17521 atgctttcac gtattttgac cagcctgaat cacggagcat tttttggttt gggttcagtc
    17581 gtggccgcaa gcgtggtgcc aaaacataaa caggccagcg cagttgccac tatgtttatg
    17641 gggttaaccc tggcaaatat cggtggcgtg ccggcggcga cctggttggg tgaaaccatc
    17701 ggctggcgga tgtcatttct ggcaacggcg gggctgggag tgatttcaat ggtaagtctg
    17761 ttcttctcat tacctaaagg tggtacaggg gcacgacctg aagtgaaaaa agagctggcg
    17821 gtattaatgc gtccgcaggt gctgtctgca ttgctgacga cggtactggg agctggtgca
    17881 atgtttaccc tctacaccta tatctctccg gtactgcaaa gtattaccca cgcaacaccg
    17941 gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat tctctatcgg taactatctc
    18001 ggcggcaaac tggcagatcg ttcagttaac ggcacgttga aaggcttttt gttgctgctg
    18061 atggtgatta tgctggcaat cccgttcctg gcccgcaatg agttcggcgc agctattagc
    18121 atggtggtgt ggggcgcagc aacctttgcg gttgtacctc cgttacagat gcgcgtgatg
    18181 catgtcgcca gtgaagcgcc aggtctgtct tcatcagtca atattggtgc ctttaatctt
    18241 ggaaatgcgc tgggagcagc tgctggtggt gcggtaattt ccgctgggct gggatacagc
    18301 tttgtgccgg tgacgggggc gattgtcgcg ggactggcat tattgctggt gtttatgtca
    18361 gccagaaaac aacctgaaac agtttgcgtt gctaacagct aattaaacaa aaacgcagaa
    18421 ggataacctt ctgcgttaca gttgctgaca acgtgcgatt tatcttgccg aatgcgctgt
    18481 taacgcctca tccgcgttgg catggtaagc gaagcgcatc aggcaatgct gcatttgcca
    18541 tcagttatta tgcagcgaga tttttcgcta cgaattccca gttcaccagc gcccagaagt
    18601 gctccagata gccaggacgt gcattgcgat agtcgatgta ataagcgtgt tcccagacat
    18661 caacggtcag cagcggagtc gcatcggtgg tcagcggagt acccgcgtta gaggttgaaa
    18721 cgatagccag tttgccatcg ctgtttttca ccagccaggt ccagccagaa ccaaagtttt
    18781 tgatcgctgc atcagtaaac tgcgctttga aatcggcaaa gctgccaaaa gatgcggcga
    18841 tagcttcagc gacttttcca gtcggttcgc caccggcgtt cggtgccagg cagttccagt
    18901 agaaagtatg gttccagacc tgagctgcgt tgttgaatac gccaccttca gagctgcgaa
    18961 taatctcttc cagtgattta ccttcaaacg cggtaccttt aatcaggttg ttcaggttag
    19021 tgacataagt ctgatgatgc ttgccgtagt gatactcgat agtttccgca gaaatgtgcg
    19081 gtgccagagc atctttagca tatggtagtg caggtaattc gaatgacatt gctactctcc
    19141 tttattatta atttgcatac gtacaatagc cttattgtgc gtatggatag ggtagcaaat
    19201 taaaaggtga gacaaaagat gaacttaccc tgttgccgta acaacagggt aaagttttag
    19261 cgaagtgttt ttggggtcat tacccgacga gcgccaacat agtggcgctg ccagtagtct
    19321 tcactgagag aagtgatttg aatttcctga cctgtgcgcg gtgactgaat aaatttgccg
    19381 ttgccgacat acacgccgac atgatcggct gtaccgcgtc cctgagtacg gaaaaagacc
    19441 aggtcgccgt ttttcagctc actacgttcg ataggtcctg catcacgcag gtgatacatt
    19501 tcattcgccg tacgcggaat acgaattttc accaaatctt tataagcgta ataaaccagg
    19561 ccgctgcaat caaaaccggt acgcggtgag ctgccgcccc aacgatatgg cttaccaatt
    19621 tgctgcatca gtttattcat tgccactttc gtggcttttt gcaccttcgc tttgtgcgcg
    19681 tcggcaaggg ttcctgacgc ggcatttttg actttcacac aatgcgattt atagccctta
    19741 cgtttggtac acttttcggt aaagctggct gaggccgttt tattgacggc gtgtttgctg
    19801 cgagttttgg cgtttttaga agcggtaatg gaagattttt taacagaaga agcggttttg
    19861 cttttggtca tacttttact ggcggctttt ttcgctgttt tctggctggc tttctgggtc
    19921 tttttggtgg ttgccgtgct tttcttttta tctgcttttt tggtgatatg agtggtagca
    19981 gaactctccc tggcttgctt tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa
    20041 agtaaagcac agagcgtgat cgaaatacgg tttatccgcg ccactgagcg tccctctgtt
    20101 aaggcaggtc tatcatgcct gcgtctgatt tacaaaactt gatgattcta atgcatagaa
    20161 gaagagaaag ttaatagctt ttattgttaa aaaatacgtt tcgttggcga gcgcaaaaaa
    20221 atgcagcagt gaaagctaaa aatggttata aatacgccag tttgccgcgc atcagagaag
    20281 aaacgaatat ttgaaatgca acaaatagag caaagggata aaatagcaaa agcgtgatga
    20341 aaatgttatt ttccgatgcc gcactgaccc gctacaatgt cagattactg ccgtaaagaa
    20401 gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa tccaacgcca gattgctgaa
    20461 aacccgatcc tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc
    20521 caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg cctatgttga tattctgcag
    20581 aatccggaca ttcgtgcgga actgccgaaa tatgctaact ggccgacctt cccgcaactg
    20641 tgggttgacg gcgagctggt cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc
    20701 gaactgcagc agctgatcaa agaaactgcc gctaaataca agtctgaaga gccggacgcg
    20761 gaataatccg tcctgcaata ctgacggatg cgaacgggca tccgtcagtc ctttatccgg
    20821 tgcgccgtaa aacctcgtcc ttcagggcgg ggatataagg cgcggttttc cacctgacca
    20881 ggtgtttgct gttgttcaat gtactggcgg atgacggata ttggcgcacc ttcgcagcta
    20941 ctggcaaaat agccaggact ccacaaaacg cctttgtagt aatacctgac tgcaatatct
    21001 ggtcgatctc gtcgcagtaa cctattcgat atgcctttga ggctgtttac cagactggat
    21061 atcgccagtt tgggaggata gttaattaac aaatggacgt gatctggttc gccatccatt
    21121 tcaaccagtt cagctgcaaa atcagcacat acatttgaaa agtaagtgcg tagtttttct
    21181 gtcgtgtcat ggtcaaaaat ctggcgtcgg tatttggtga caaagaccag gtgaacatgc
    21241 atcaggaaaa cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaagt
    21301 atcatttgac ctgtgaaacg attacaggca tttaaattcc agttaagacc cggtggtcaa
    21361 caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt tcgttttcaa tcgtgctctg
    21421 gcgcttcaga atgagaatca tgaggccggt aataaataca tcccttacgg gaaaatggct
    21481 tcctggctgg ttgagtggaa aaacgccact gaaacgcaat ggcttaaaga ttcgccctca
    21541 cagccattgc aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccagaag
    21601 cgggctgctt ttccccgatt caaaaagcgg ggacagaatg ctgcattccg ctacccgcag
    21661 gacgttaagc tcgatcagga aaacagccgt atttttctgc cgaaacttgg ctggatgcgc
    21721 taccggaata gccgtcaggt cacgggagtt gtgaaaaatg tcactgtcag ccagtcctgc
    21781 ggtacgtggt acatcagtat tcagacagaa agtgaagtat caactccggt tcacccttca
    21841 gcatcaatga tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca
    21901 gtctttgagc ctgtaaacag ttttcaaaaa aaccagaaga cgctggcgag acttcagcga
    21961 cagttaagcc g
//
LOCUS       DAJVPT010000067        21335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVPT010000067 DAJVPT010000000
VERSION     DAJVPT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..819)
                     /gene="fbp"
                     /locus_tag="OQM47_002491"
     CDS             complement(<1..819)
                     /gene="fbp"
                     /locus_tag="OQM47_002491"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1480604.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLL"
     gene            995..2368
                     /gene="mpl"
                     /locus_tag="OQM47_002492"
     CDS             995..2368
                     /gene="mpl"
                     /locus_tag="OQM47_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1480605.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2451..3002)
                     /locus_tag="OQM47_002493"
     CDS             complement(2451..3002)
                     /locus_tag="OQM47_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1480606.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3096..4448
                     /gene="pmbA"
                     /locus_tag="OQM47_002494"
     CDS             3096..4448
                     /gene="pmbA"
                     /locus_tag="OQM47_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1480607.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4631..5013
                     /gene="cybC"
                     /locus_tag="OQM47_002495"
                     /pseudo
     CDS             4631..5013
                     /gene="cybC"
                     /locus_tag="OQM47_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5058..5522)
                     /gene="nrdG"
                     /locus_tag="OQM47_002496"
     CDS             complement(5058..5522)
                     /gene="nrdG"
                     /locus_tag="OQM47_002496"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1480608.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5680..7818)
                     /gene="nrdD"
                     /locus_tag="OQM47_002497"
     CDS             complement(5680..7818)
                     /gene="nrdD"
                     /locus_tag="OQM47_002497"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1480609.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8212..9867)
                     /gene="treC"
                     /locus_tag="OQM47_002498"
     CDS             complement(8212..9867)
                     /gene="treC"
                     /locus_tag="OQM47_002498"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1480610.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9917..11338)
                     /gene="treB"
                     /locus_tag="OQM47_002499"
     CDS             complement(9917..11338)
                     /gene="treB"
                     /locus_tag="OQM47_002499"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1480611.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11457..12404)
                     /gene="treR"
                     /locus_tag="OQM47_002500"
     CDS             complement(11457..12404)
                     /gene="treR"
                     /locus_tag="OQM47_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1480612.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12589..12642
                     /gene="mgtL"
                     /locus_tag="OQM47_002501"
     CDS             12589..12642
                     /gene="mgtL"
                     /locus_tag="OQM47_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1480613.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12783..15479
                     /gene="mgtA"
                     /locus_tag="OQM47_002502"
     CDS             12783..15479
                     /gene="mgtA"
                     /locus_tag="OQM47_002502"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1480614.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15685..16071)
                     /gene="ridA"
                     /locus_tag="OQM47_002503"
     CDS             complement(15685..16071)
                     /gene="ridA"
                     /locus_tag="OQM47_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1480615.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16144..16605)
                     /gene="pyrI"
                     /locus_tag="OQM47_002504"
     CDS             complement(16144..16605)
                     /gene="pyrI"
                     /locus_tag="OQM47_002504"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1480616.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16618..17553)
                     /gene="pyrB"
                     /locus_tag="OQM47_002505"
     CDS             complement(16618..17553)
                     /gene="pyrB"
                     /locus_tag="OQM47_002505"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1480617.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17557..17691)
                     /gene="pyrL"
                     /locus_tag="OQM47_002506"
     CDS             complement(17557..17691)
                     /gene="pyrL"
                     /locus_tag="OQM47_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1480618.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(17972..18367)
                     /locus_tag="OQM47_002507"
     CDS             complement(17972..18367)
                     /locus_tag="OQM47_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1480619.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18499..19212)
                     /gene="bdcA"
                     /locus_tag="OQM47_002508"
     CDS             complement(18499..19212)
                     /gene="bdcA"
                     /locus_tag="OQM47_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1480620.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19283..19876
                     /locus_tag="OQM47_002509"
     CDS             19283..19876
                     /locus_tag="OQM47_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1480621.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20021..20473
                     /gene="tabA"
                     /locus_tag="OQM47_002510"
     CDS             20021..20473
                     /gene="tabA"
                     /locus_tag="OQM47_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1480622.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20596..>21335
                     /locus_tag="OQM47_002511"
     CDS             20596..>21335
                     /locus_tag="OQM47_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480623.1"
                     /translation="MGKISDLNYSQHITLADNFKQRNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5158 a         5272 c         5619 g         5286 t
ORIGIN      
        1 aagcaaacgc agtttgccgt ccgggtggct ggcggtgctt gggtagagat aaataccgcc
       61 tttcagcagg ttacggtgga aatccgcaac cagtgaaccg atataacgcg aggtatacgg
      121 gcgattggtg gatttatctt cttcctggca gaatttaatg tacttcttca ccccgttcgg
      181 aaacttaatg tagtttcctt cgttgatgga gtaggttttg cctttctccg ggaagcgcat
      241 ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg taagtaaagg cgtgaacacc
      301 gcatccggtg gtgtaaacca gcatggtaga ggagccgtat accacgtaac ctgccgcaac
      361 ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac
      421 gcggcggtag atggagaaaa tggtaccgac agagacgtta acatcgatgt tggacgagcc
      481 atccaggggg tccatcagca ccacgtattt tgcgtgttca cagccttcaa agacgacaat
      541 ctcatcttct tcttcagagg caatccccgc aacgatatcg cgtgctttca gtgcggcttt
      601 cagtttttca ttagcgaaca agtcgagttt ttgctgaacc tcgccctgca cgttctcagc
      661 accgctggca cccaggatat caaccagtcc tgctttgttg atatcgcgat ggataatctt
      721 ggcgcccagt tttattgccg acagcaaagc agtgagctca ccggtagcat gagaaaactc
      781 gtgctgcttt tcgacaataa attcacctaa cgttttcata aaactttccc tgcaatgttt
      841 atggagtaaa gcgaccgcaa caatcttaac aaataatctc aatgttgcgc tcaggtgaat
      901 cgcgccagca aattgcggat tatcctgaaa tgcgtttctc acttgcccga catatgcgta
      961 aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc attcatattt taggaatttg
     1021 tggcacattt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg aagtaacggg
     1081 ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag gcattgagct
     1141 gattcagggt tacgatgcca gccagctcga gccgcagccg gatctggtga ttattggcaa
     1201 cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat
     1261 gtcaggtcca cagtggctgc acgattttgt gctgcgcgac cgctgggtgc tggccgttgc
     1321 cggtacacac ggcaaaacca ccaccgcggg aatggcgacc tggattctgg aacagtgtgg
     1381 ttacaaaccg ggatttgtga tcggcggtgt gccggggaac tttgaggttt cggcgcgtct
     1441 gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct tcttcgacaa
     1501 acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc ttgagttcga
     1561 tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc atctggtgcg
     1621 tatcgttccg gggcagggcc gtattatctg gccggaaaat gacatcaacc tgaaacagac
     1681 catggcgatg ggctgctgga gcgagcagga gctggtgggt gagcagggtc actggcaggc
     1741 gaaaaagctg accaccgatg cttccgaatg ggaagttttg ctggatggcg aaaaagtggg
     1801 cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga tggcgattgc
     1861 agcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg gttcgtttat
     1921 taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg tatatgacga
     1981 ttttgcccat cacccgacgg cgattctggc aacgctggcg gcgctgcgtg gcaaagttgg
     2041 tggtacggcg cgcattattg ctgtgctgga accgcgctcg aataccatga aaatggggat
     2101 ctgcaaagac gatctggcac cttcattagg tcgtgctgat gaagtcttcc tgctgcaacc
     2161 ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg
     2221 gagtggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc ctggcgacca
     2281 tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc tggatggtct
     2341 ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc ctgatatagc attgccggat
     2401 aaggcgttta cgccgcatcc ggcaaaccat actcacatca acaacgaaaa ttacccttca
     2461 ttctccgcca gttcgcgcag atactggaaa atctggcgtg cggatttcgg cggcttattc
     2521 ccttctttct ctttcttcgc gttgcggatc agtgtacgca gttgctgacg atcggcatcg
     2581 ggccacagat tcaatacttc agcgatggcg tcatcacctt gatcgatcag acgatcgcgc
     2641 aggttttcca gtttatggaa cagcaccacc tgctggttgt gacggttttt cagcttatcc
     2701 agcgcctgac gaataggctc tacgtcgcgc tggcgcagca tcttaccgat gagctgcaac
     2761 tggcggcggc gaccttccat cttaatacgc tgggcgagct caatagccgg gcgcagatcc
     2821 gcatctaacg ggatcttatc cagcgcgttt ttccccagat caacaatttc cgcgccaagg
     2881 cgttttagct cctcggcatc acgtttaatt tcacttttac tgacccagat aatttcatcg
     2941 tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc agtcttcggg ctgcttagtc
     3001 atctcaggct ccttaaaaaa agaggctaat gttaccagtt aagatgcgca ctgaaaaacg
     3061 gttctctgtt agacttcaga gaaactctct acattatggc acttgcaatg aaagtaatct
     3121 ctcaagttga agcgcagcgc aagattctgg aagaagcagt ttccactgcg ctggagttgg
     3181 cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa gaccaccggc attagcgtaa
     3241 gcacgcgtta tggtgaagtg gagaatgttg aattcaatag tgatggcgcg ctggggatca
     3301 ctgtttatca ccagaaccgt aaaggtagcg catcgtctac cgatttaagc ccgcaggcca
     3361 ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta tacctcgcca gatccctgtg
     3421 ccggcgtggc agacaaagag ctgctggcct ttgacgcacc gaatctcgac ttgttccacc
     3481 ctgcggaagt ttccccggat gaagccattg aactggcggc ccgcgcagaa caggcggcat
     3541 tgcaggcgga caaacgcatc accaataccg aaggtggcag ctttaacagc cactacggtg
     3601 tcaaagtttt tggcaacagc cacggcatgt tgcagggtta ctgctcaacg cgtcattcgc
     3661 tctccagctg tgtaattgcc gaagaaaatg gcgatatgga gcgtgattac gcctacacca
     3721 ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt cggggccgac tgtgctcgcc
     3781 gtactttatc gcgcctgtca ccgcgtaaac tctccaccat gaaagcgccg gtcatttttg
     3841 ccaatgaagt ggcaaccggg ctttttggcc atctggtggg ggcgatagcg ggtggctcgg
     3901 tttaccgtaa atctaccttt ctgctggatt cgctgggtaa acaaattctg ccggactggc
     3961 tgaccattga agagcatccg catctgctga aagggctggc gtcgacgcca ttcgacagcg
     4021 aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg catcctgact cagtggctgc
     4081 tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac cggacatgcg ggcggtattc
     4141 acaactggcg gattgccgga caagggctaa gcttcgaaca aatgctcaaa gagatgggca
     4201 ccgggctggt ggtgacggag ctgatgggcc agggcgtgag tgccattacc ggtgattatt
     4261 cccgtggtgc agcgggcttc tgggtagaga atggcgaaat tcagtatccc gttagtgaaa
     4321 tcaccatcgc aggtaattta aaagatatgt ggcgcaatat tgtcaccgtc ggtaacgata
     4381 ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt gccggagatg aaaatcgccg
     4441 ggcagtaaaa aactggcgcg acctgccgcg ctataaacta aaaaattaca caaaatcatt
     4501 cgcactgcat cgagacggaa agtgaatgag ggccggggag cgtactcgca gtacgtgacc
     4561 cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg atgaagtgta aataaaaaag
     4621 gaagtgagca atgcgtaaaa gcctgttagc tattcttgca gtctcctcgt tggtattcag
     4681 ttctgcgtcg tttgccgctg atcttgaaga caatatggaa accctcaacg acaatttaaa
     4741 agtgatcgaa aaagcggata acgcggcgca agtcaaagac gcgttaacga agatgcgcgc
     4801 cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc gaagataaat caccggacag
     4861 cccggaaatg aaagatttcc gccacggttt cgacattctg gtcggtcaga ttgacgacgc
     4921 gctgaagctg gcaaatgaag gtaaaaagaa gcgcaggctg ctgcagagca actgaaaacg
     4981 acccgcaacg cctatcacca gaagtatcgt taattcctca tttccctgtt gcctgcactc
     5041 aggtaacagg gaacttatca tcgcaaatga tgcaccacct gattgctgct gccgcgccag
     5101 atcagggaag ggtcttttaa atcctgcaca aatttgccgt cgaccagcac gttaatcaaa
     5161 tcaacaacct gcatttgcgc agcgttgagt tcgtcgagtt tatagcctgt ccacacccag
     5221 atgtctttac ccggacactc ggcgcggatg cgttttacca gtttcagaat atccggcacg
     5281 ttttgcggat gcagcggatc gccgccggag agggaaatcc cctggcgttt gatacgagtg
     5341 tcattcagat ctttaatgat cttgtcttcc attgctttgg taaatggctg accagaattt
     5401 acccgccatg tgcttttgtt atagcaaccg gggcattcat gaacacaccc ggagacaaac
     5461 agggtgcagc gagtgccggg gccgttgacg atgtcgacag gatagtactg atgataattc
     5521 attatttcgc ctggcttatc agtccgcgtg taggctgcat ccggtaatca atgcctgatg
     5581 cgacgctgtc gcgtcttatc aggcctacag ccgttgccaa atgtaggccg gataaggcgt
     5641 ttacgccgca tccggcattt gcttagcgca gagtgaagat taacctatct gcccattccc
     5701 caaatgttta acgcggcgct taacttcttc ctgcttacca gcgttaaacg gacgtgcatc
     5761 cgggctacct aaatatccgc acacgcggcg agttaccgac acacgggagg cgtcatggtt
     5821 accacatttc gggcaagtga agcctttgct ggtgcactcg aactcaccgg taaagccaca
     5881 ctcgtagcac tcatcaatcg gtgtattggt gccgtaatac ggtacatgct gatagctgta
     5941 atcccagaca tcttccagcg ctttcaggtt gtgctgaatg tttggatact cgccgtagca
     6001 aatgaaacca ccgttcgcca gcggcgggta aggcgcttca aagtcgatct tgtcgtacgg
     6061 gttcaccttc ttctccacat caaggtggaa actgttggtg tagtaacctt tatcggtcac
     6121 gcccggcacc acgccaaact cagcagtatc gagacggcag aagcggtcgc acaggttttc
     6181 actcggcgtg ctgtaaagac tgaaaccata acccgtttct tctttccact gatccactgc
     6241 ctgacgcaga cgttcaacaa tggcgatacc tttcgcgcga agcttctcgt tgtcgtaaac
     6301 atgctcgccg ctgaacagcg cgttaatggt ttcgtggatg ccgatgtaac ccagcgaaat
     6361 agacgcacga ccgtttttga agatttcaga aacatcatcg tcagcattca gacgtacgcc
     6421 acaagcacct tccatataga ggatcggggc gacgcgcgct ttcacgcctt cgagacgagc
     6481 gatacgggtc atcagtgcct tacgtgccag caccagacgt tcatccagca gcttccagaa
     6541 ggtggcttca tcgccttttg cttccagagc aatacgcggc aggttcaggc tgatcacgcc
     6601 gaggttgtta cgaccatcgt ggatctgctc gccgttttca ttttcccaca cgccgaggaa
     6661 gctgcggcag cccatcgggg ttttaaacga accggtgacg ttcactacct gatcgtagtt
     6721 caggatatcc ggatacatgc gcttgcttgc gcactccagc gccagctgtt tgatgtcgta
     6781 gttcggatcg ccttttttat ggttcaggcc atcgcgaatc gcaaacacca gtttcgggaa
     6841 caccgcagtt ttacggtttt taccgaggcc tgcgatacgg ttacgcagga tcgattcctg
     6901 aatcaggcgc gattcccagc tggtgcccag accaaaacca aaggttacaa acggcgtctg
     6961 accgttggcg gtgtgcaggg tgtttacttc gtactccagc gactggaagg catcgtagca
     7021 ctctttgatg gtacgagagt tagcgtagcc ttcggcgtcc gggatgttcc actcttctgc
     7081 ggtcttgcga tgtttgttgt agctggcggt aacaaacggc gccagcactt catcgatacg
     7141 gttaatggtg gtgccgccat aaatatggct ggcgacctga gcaataatct gcgcagttac
     7201 cgcggttgcc gtagagatcg acttcggcgg ttcaatctcg gcgttcccca ttttaaaacc
     7261 ctgggtcagc atgcctttca ggtcgatcaa catgcagttg aacatcggga agaacggcga
     7321 gtaatcgaga tcgtgatagt gaatatcgcc acgctcatgt gcctgcacca cgtcacgcgg
     7381 cagcaggtgc tggcgtgcat agtgtttagc cacgatcccg gccagcaggt cgcgctgggt
     7441 tggaatcacc ttgctgtctt tgttggcgtt ttcgttgagt aacgaggcgt tggtctgctc
     7501 gaccagacca cggatctcct ggttcaggcg accgcgtttt tcacgttcaa tgtcgcgatc
     7561 gtgacggtac tcgatgtaag cacgagccag ttgtttgtat ggacccgaca tcagctggtt
     7621 ttcaactgcg gtctggatct cgttgatatc cacctggttg cgcccctgca tctgctcgtt
     7681 gacaaccgcg gcaacagtgg cgcaataatc ggcatcatcg acttccgctg ctttagctgc
     7741 acgcagaatc gcttctttga tgcgctctga tttaaacggc actttgcagc cgtctcgttt
     7801 catcacatgc ggtgtcatga tcgctccata tttttaagaa caggttatcc acagaaattg
     7861 ggaaaggcgt tttccggttt tttcgtttcg ttttccggtg ctttccgcaa tcgccatccg
     7921 cttatccaca acgccgcacc tttttcgtgg ggagctgtag tagcaattat agtcgattaa
     7981 tacaacatat tgggttggga cgcattttaa agtctatata tagtgctttg catcaaggat
     8041 gttttaactt tttttgatgt agctcaaagt aaaaagcaga gcgtacggat gacgggcgct
     8101 acagcgttat gtaaattttt taatgaattt gctggttgaa aaatcaacaa aaacaacatg
     8161 ctgacagaca aaaccccgga atgaccggca ttatcggggc ttagggaaga tttacttctg
     8221 taaccaccag acagcctcaa aaggccgtaa attcatggca cagggttgtg gtgaggcttc
     8281 ttcgtagtta tgcatcacaa gctgccagtt gccgcgcatt tgccctggct gccagggttg
     8341 gatctcacgg ctaaggttgg caatgaccag caaggtttgc cccttccatt cacggcgata
     8401 gcaccacaat acagggctgt ttggcagcag atcctggtaa ttgccccatg tcaggatggc
     8461 ttcttgctta cgcagtgcga ttaacttttg gtaggtgtaa aacacagagg agtcatcggc
     8521 cagcgcggct tctacgttga tttgttgata gttatcgcct aggccaatcc acggttcgcc
     8581 agccgtaaac ccggcattat cgccgttgct ccattgcatg ggcgtgcggc tgttgtcacg
     8641 ggatttactg gcgaggattg ccaataactc gtcggcatca cgaccatcgt tgcgcagctc
     8701 ggcaaacata ttgaggctct ccacgtcgcg atagtcagta atgcgcgtga aatgcgggtt
     8761 ggtcatgcca atctcttcgc cctggtagat atacggcgtt ccctgcatgc catgcagcac
     8821 catcgccagc atttttgccg caggcacgcg gtattcacct tcatcaccaa agcgagaaac
     8881 aatgcgcggc tgatcgtggt tacaccagaa caaggcattc catgccacgt tgtgcattcc
     8941 ttgttgccag tggcggaaca atgttttcaa cgccacaaag tcaggtttag ccagcgtcca
     9001 tttttcacca ccgggataat cgaccttcag gtgatgaaaa ttaaaggtca tcgacaattc
     9061 actgcctgtc agtgccgcgt atcgctggca atgctcaagg ctggtggagg acatttcacc
     9121 tacggtcatt aacccgcgtg gcgtaaacac atcgcggttc atctcgtgca aaaactcgtg
     9181 tgctcgtggc ccgtcggtgt agaagcgacg cccgtcgcca tccaggtctt cagggaaacg
     9241 cgggtctttg gagatcagat tcaccacatc caggcgcaac ccgtcgaccc cacgatcggc
     9301 ccagaattca cagacttttt tcagctctgc gcgtaccgct ggattctccc agttgagatc
     9361 cgcctgttct ggtgcaaaga gatgcaaata gtactgttcg ctttccgcat gccagcgcca
     9421 cgcactaccg ccaaattttg aacgccagtt gttcggtggc gtttctggtt cgccatcgcg
     9481 ccagatataa aactggcggt aagggctttc tttgttcagc gcctcgcgaa accaggcatg
     9541 ttgggtagag gtatggttaa acaccatatc gagaatgata cgaatcccgc gcgattttgc
     9601 ctgcgtcacc agttcgtcaa aatcgtccag cgtgccgtag gtgggatcaa tcgccgtata
     9661 gttcgctaca tcgtaaccgt tatcgacctg gggagagaca taaaaggggg ttagccagat
     9721 ggcatcaacg cccagtttat gcagatagtc caggcgttgg ataacgccac gtaaatcgcc
     9781 ggtgccgcta cccgtggtgt cctgaaaact ctttggataa atctggtaga taacgccgtt
     9841 ttgccaccag tggggaagat tagtcattac gttattcctg caaatgcgag ggggcgcaat
     9901 tgcgccccga agaaaattaa acaatgtcca gcgtgcccag gcggtatttc cgctgataga
     9961 taaacgaggt gagtacaatc gggatgatga tggcgatagc cattgccagc gcaaatacct
    10021 gccagtagct cggttgaata gagagaattc ccggcaggcc ccctacgccg ataccattcg
    10081 ccataacgcc gttcaggccg catagcaatc ctgccagacc agaaccaatc atcgcgcaca
    10141 gcatcgggaa gcgatatttc aggttgatgc cgtacattgc aggctcagtg accccaagcc
    10201 aggcggagat agcggcaggc acggagatct cgcgttcatt gtgcttgcgg ctggaaatga
    10261 taatgcctat cacggcggag ccctgagcga tattcgacag cgcaatcagc ggccacactg
    10321 gcgtgccacc catgctttga atcatctgca aatcaatagc aagggtggtc tggtgtacgc
    10381 cggtgatcac cagcggcgcg tacaggaagc caaacaatgc cgcaccaatc ggagcaaagc
    10441 tgccggtcat caggtgacgt accgcaaagg caacgccatc gccaatcatg cgaccaaacg
    10501 gaccaatcag cgcatgggcg aggaacaccg cgaggatcag cgaacagacg ggcaccacca
    10561 ccagatagag gtaatccggt acgatgcgtt taaggcgagt ttcaataacg cccagcgcca
    10621 gcccggctaa cagtgccggg atcacctgcg cctgatagcc cactttggcg atgctgaaca
    10681 tgccaaagtc ccacacttcc ggcagctgct gcccgagcag ataagcgttc atcagctggg
    10741 gggaaaccag tgtcacgcca agcacgatac caaggatcgg cgtgccgccc atttttttca
    10801 ccgctgacca gcaaatcccg accggcaggt agaagaaaat cgcttcaccg atcaaccaca
    10861 gaaaatcgta gatcgtttgc agggaagggt acatttgcgc cagcgtctga ccgttgctca
    10921 tgggcaaatc gccgatcaca ttgcgaaaac cgaggatcaa accgccgcta atcaacgcgg
    10981 gcagcaacgg gaagaagatc tccgcgaaat gagagatcaa ctgctcatgc catttcatat
    11041 tttgccgggc ggcttttttt acctgctctt tatcaacctg cgcctgtccg gttgacgcta
    11101 ttagtgcttg atagtaatca cccacgttgg tgccaatcac cacctgaaat tgcccggcat
    11161 tggtgaaaca gcctttcacc atggggagtt gctcaatttc tttcggtctg gcattggccg
    11221 gttggttgag gacaaagcgc aggcgagtaa tacagtggct caccgtcgca atattgccgc
    11281 gcccgccgac cagttcaatc aaccgatcga tatccgtttg gtttattttg ctcatcataa
    11341 agccccatgg cagatgacat ttttggttgg ctgcagaata tattgcgcgg aaaaatttaa
    11401 aaacgggaac gttcccgaaa cgcagcgaag atcacaattt atcgttcagg aaacgatcag
    11461 gacagggtgg cggggatgat gatttgttgc ggttcgctgc gcccggttac ctgtgcgatc
    11521 aattggcagg ccgcctggcg tccagcttcg gcgtagccag gatctacggt gacgatctcc
    11581 ggatgaagga atttcattaa cggcgtatta cctacgctcg ccagttgcaa ggtgtcgatg
    11641 cgttgctctt gcaggtattt acttgcgcca agagccagcg tgtcggttgc gcacagtaac
    11701 gcggtagttt caggcgtaat cacttttgct acgttttcat agccttgctt catagcaagc
    11761 cctggcagag cggcaacggg atgcagttta tgcgctttgc agaacgccag gtaggcttcg
    11821 tgacgtcgct tcccggtcgt cacgtcacta tgcggcacgc cgagataact gatattgcga
    11881 tgcccctggt catagagccg ttgcatcaga atcttgattg ccccttcgtc gtcataacag
    11941 accgaagcaa agccttttgc gtcacgcgcc agcagaacca gcgatgactg ccagtgggct
    12001 aacatttctt cggttatgcc ggtaaaaccg aacagcacta cgccgtcgat attacgccgt
    12061 ttcagcaccc ccaaatgttc ggcaactaat tgtggggaaa actgattttc catcatgatt
    12121 ggatcgtaac cttgttcata gaacgctggc agcatggttt gaacggcgag attttctgac
    12181 aacgaatcca gacgggtaac aatgatggcg accactttat cgctttgccc acgcatagcg
    12241 cgcgcagagc gggaagggga aaatccatgc tgattcatca ctgcttcaac acgctcgcgc
    12301 gtgcgctgac tcacgccgct ttcgttattc agcacccggg aaaccgtaga tttccccacg
    12361 ccgcttaagc gcgcgatgtc tttgatggtc agccgatttt gcatcctgtt gtcccataac
    12421 gtgttgttta attatttgag cctaacttta cccgtgcatt cagcaatggg taaagtctgg
    12481 tttatcgttg gtttagttgc cagcaggtat tatatcgcca taaatgctac gaatattatt
    12541 ggattctcct tattatttgc ggcgcttttt tcacttaccg gaggttatat ggaacctgat
    12601 cccacgcctc tccctcgacg gagattaaaa cttttccggt aagcccgtct tttcacggcg
    12661 ttaccggatg cgtaaggccg tgacgtttta atgtccctgc tcagctttat taccttcagg
    12721 taaggcttcg ccacgcctga agacatttct gtactgtttc agacagtgcg gagggactcc
    12781 ttatgtttaa agaaattttt acccggttca ttcgccattt accttcccgt ctggttcatc
    12841 gcgatccatt gcctggcgcg cagcagacag tgaatacggc ggtcccgccg tccttaagtg
    12901 cgcattgcct gaaaatggcg gtgatgcccg aagaagaatt gtggaaaacg ttcgacaccc
    12961 atccggaagg attaaatcag gcggaagtgg aatctgcccg cgaacaacat ggtgataata
    13021 aattacccgc ccaacaaccg tcgccgtggt gggtacattt atgggtctgc tatcgcaacc
    13081 cctttaatat tttgctcacc attcttggtg ctatttctta cgccacggaa gatttatttg
    13141 ccgcaggtgt tatcgcgcta atggtcgcta tttctacgtt gctgaacttt attcaggaag
    13201 ctcgttccac caaagcggca gatgccctga aagcgatggt cagtaatacc gcgacggtac
    13261 tgcgagtaat taacgacaaa ggcgaaaatg gctggctgga gatcccgata gaccagctgg
    13321 tgcccggcga tattataaaa ctggcggcgg gggatatgat cccggcagat ttacgtatct
    13381 tgcaggcgcg ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct ctgcccgtgg
    13441 aaaaagccgc tactactcgc cagccggagc acagcaatcc gctggagtgc gacaccctgt
    13501 gttttatggg caccaccgtg gtgagcggca cggcacaagc aatggtgatt gctacaggag
    13561 ccaacacctg gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc gagccgaatg
    13621 cctttcagca agggatcagc cgcgttagta tgctgctgat tcgctttatg ctggtgatgg
    13681 ctccggtggt gttgcttatc aacggttaca ccaaaggcga ctggtgggaa gcggcactgt
    13741 ttgcgctttc ggtcgcggta ggcttaacgc cggaaatgtt gccgatgatt gttacctcga
    13801 cgctggcgcg cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa catctggatg
    13861 ccattcagaa ctttggcgca atggatattc tgtgcactga taaaaccggc accctgacgc
    13921 aggacaaaat tgtgctggag aatcataccg atatctccgg taaaaccagc gaacgcgtgc
    13981 tgcatagcgc gtggttgaac agtcattacc agaccggact taaaaatctg cttgataccg
    14041 cggtgctcga aggtacggat gaagagtcag cgcgttcgct ggccagccgt tggcaaaaaa
    14101 ttgatgagat tccgtttgat ttcgagcgtc gtcggatgtc ggtggtagtg gcagaaaata
    14161 ccgaacacca tcagctggtt tgcaaaggtg cattgcagga aatcctcaat gtgtgttcgc
    14221 aggtgcgtca caatggcgag attgtaccgc tcgatgacac catgctgcgt aagattaagc
    14281 gggttactga tacgctgaat cgtcaggggc tgcgcgtggt tgcggtggcg acgaaatacc
    14341 tgccagcgcg tgaaggagat taccagcggg cggatgaatc cgacctgatc ctcgaaggat
    14401 atattgcttt ccttgatccg ccaaaagaga caaccgctcc ggcactgaag gcattaaaag
    14461 cgagtggaat taccgtaaaa atccttactg gcgacagtga gttagttgca gcgaaagtgt
    14521 gccatgaagt ggggctggat gcgggagagg tggtcattgg tagtgatatt gaaacgctat
    14581 ctgacgatga actggcaaat ctcgcacagc gcaccacgct gtttgcccgc ctgacgccga
    14641 tgcataaaga acgcattgtg actttactga agcgcgaagg gcatgtggtt ggctttatgg
    14701 gcgatggtat taatgatgcg cccgccttac gcgctgcgga tatcggcatt tctgtggacg
    14761 gcgcggtaga tattgcccgt gaagcggctg atatcatcct gctggaaaaa agcctgatgg
    14821 tgctggaaga gggggttatt gagggacgtc gcactttcgc caacatgctg aaatacatca
    14881 aattgacggc gagttctaac ttcggtaatg tgttcagcgt gctggtggcg agtgctttct
    14941 tgcccttcct gccgatgttg ccgttacact tgcttattca gaacctgctg tacgatgtgt
    15001 cacaggtggc gatcccgttt gataacgtcg acgacgagca aattcaaaag ccgcagcgtt
    15061 ggaatccggc ggatctgggg cgctttatgg tcttcttcgg accgatcagc tcgattttcg
    15121 atattctgac gttttgcttg atgtggtggg tattccatgc caacacgccg gaaacgcaaa
    15181 cgctgttcca gtcgggctgg tttgtggtgg gattactgtc gcaaacgctg attgtgcata
    15241 tgatccgtac ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg ttaatgatca
    15301 tgaccgtgat cgtgatgatt gtcgggatcg cattgccgtt ttcaccgctg gccagttatc
    15361 tgcagctgca ggcgctgccg ttaagctatt tcccgtggct ggttgcgatt ctggcagggt
    15421 atatgacgtt aacccagttg gtgaaagggt tctatagccg tcgttacggc tggcagtaaa
    15481 gaataaactg ggcatggtgg cccggattca gattgcagac aaagtgagct ttgttcatgc
    15541 cggatgcggc gtaaacgccc aatccggcct acaaattcaa taaattgcag gaatcacgta
    15601 ggcctgataa gcgtagcgca tcgggcgatt ttgcttttgt catcagactt aatccgggca
    15661 tgatagcccg gatttccatc aagattagcg acgaacagcg attgcttcga tctcaatctt
    15721 cacgtctttc ggcagacggg caacttcaac gcaagaacgt gccgggaaag tggcgttgtg
    15781 ttcggtgaag aaggcttcgt aagtggcgtt tacggttgcg aagtcgttca gatctttcac
    15841 gaacacagtg gttttaacga tgtcgcccac tttcaggcca gcggcttcga cgattgcttt
    15901 tacattatcc agcgactgac gtgcctgtgc agcgacgtct gccggtactt caccagtttt
    15961 cggatttacc gggatctgac cggaggtgat gatcatattg cccagatcaa cgccctgtac
    16021 gtaaggaccg atagctgccg gtgcattttc cgtcgcgata gttttgctca tgatttctcc
    16081 tttattacag cggtaaaaag tctggctcat tatagggagc cagactttta ttaccaaccg
    16141 caattaattg gccagcacca cattatggga aaactctttt tcacagtatt tgcatttaag
    16201 cgcgatgtca ttggcgcgtt ttcgcacggc aaagctggat gaaaccggtt cggcatggct
    16261 gatacagttg ctgttcgggc agaccagcac attgtcgatg cgctctggca gacttgggcg
    16321 cgatttaccc accacttcat agttgtcgat acggttaacc gtggcttgcg gtgcatacaa
    16381 tgccagttgg tctacttgct cttcactcaa aaaggtattt tcgattttga tcagatcttt
    16441 gcggcccatc tcgccagaag gcaaattcag accaatggtg atgcgctgat ctgtttcggt
    16501 caacttgaac agactcaaca gcttaaaacc gatctgggcg gggatatggt caattaccgt
    16561 gccgcgttta atagcttcaa cctgcaattt attatcgtgt gtcatctcta tttcccctta
    16621 cagtaccaga tcgcgattca gaaccagtgc cagtaacgcc tggcgagcga aaatcccgtt
    16681 gcctgcttgc tggaagtacc aggcgtgagg cgttttatcc acatccgtcg caatctcatc
    16741 aacacgcggc agcggatgca gcactttcat attggctttg gcgttgtgga gatcgctggc
    16801 gcgaagaaca aactgcgctt tcacgttggc gtactcggac gggtccagac gctctttttg
    16861 cacgcgggtc atgtacagga tgtctacttc cgccatcact tcttcaatag agctgtgcag
    16921 actccaggcg atcccttttt catcgagcat atccagaatg tattgcggca ttgccagcgc
    16981 atccggcgcg ataaagtaaa aacggttgcc gtcgaacttc gctagcgcct gggtcaggga
    17041 gtgaacggtg cggccatatt tcaggtcacc aaccattgcg acgtggagat tgtccagacg
    17101 cccctgggtt tcctgaatgg tgaataagtc cagcaaggtt tgcgtcggat gttggttgga
    17161 gccatcaccg gcattcagta ccggtacatt gccagaaaat tcggtggcta ggcgtgccgc
    17221 accttcctgc ggatgacgca tcactatcgc atcgacgtaa gtgctgataa ccgaaatggt
    17281 atcggccagc gtttcgcctt ttttacccag tgatgtattg gcgctgtcgg agaagcccac
    17341 cacgctggca cccaggcggt gcatggaagt ttcgaaagag aggcgggtac gggtagaggc
    17401 ttcgaagaaa cagctggcaa tgactttgtg cttcaacagc tctggttgcg ggtttgcttt
    17461 cagtttcgcc gctgtcgcca gcaccagatt aaggtcatcg cgactaaggt cgtttatgga
    17521 aatgatatgt ttctgatata gcggattagc catcttttat ctcctgacac ctaggcaaaa
    17581 aaaagcccct cgattgaggg gctgggaatg ggtgatcaac gggaagaaaa acggcaggcc
    17641 agcgtctttt ttcagacgcg gtaagacaaa atgtcgaaca cactgaacca tacatcctcc
    17701 cggcaaattg tccggcatta tactcatagc cagaagcggt gcaagcattt gatgcaatat
    17761 tttgtcagcg caaacggttt atttgaatta aaagtcaaaa tatatgcatt tttattttct
    17821 atgattctgg aggggaagtg tcgttcggta tcaataattg cagacatttc ctgctttttt
    17881 ctttttcatt ttcacgcaat cagaaatgca ttttattcca ttcatccgac ttatagggcg
    17941 gagggtgaaa acgaacggct aacactattg cttatagctc agggatacgc gctatcactt
    18001 taatttcaaa atcaaagcct gccagccagg taacacccac cgccgtccag tttggataag
    18061 gtggggcgct aaatatttca tttttcaccg tcatgatgtc ttcagattgt ttttccggat
    18121 cggtatgaaa gctcgtaaca tcaatgatat cgtcaaaagt gcatcccgca gctgccaggg
    18181 tcgcatgcaa attatcaaag gccagtctga cttgttgctg aaaatcgggt tctggtgttc
    18241 cgtcctctcg acttcctact tgcccggaaa caaacagcaa atcgccggaa cgaatagccg
    18301 cagaataacg atgctcagca tacagtgaat gtcggccagc agggaaaaca gcggttcttt
    18361 ctaccatttg gttatcctca agatttacga catgaacaga agatttatat ttatcgggag
    18421 ccgcttttag ggggacgacg ttagtaaaca aaacccagac atcatggata atggctgggc
    18481 ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca atggtatgca tcgcgccggt
    18541 aacaaaactg gcttctggcc ctgctaacca tgcgaccata ccagcgacct cttccggttg
    18601 cccatgtctt ttgatggcca taaaactatg caacatatcg cgcattggcc cgttggcggg
    18661 attaacgtcg gtatcaattg gtcctggctg gacgacgttg atggtgatcc ctcgcggtcc
    18721 aaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg cggcataagc
    18781 agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta agatgcgccc
    18841 gccttcgggc atctgccggg cggcttcaac agaggcatga taaggagcat gaatattgat
    18901 tttgaaaagg cgatcaatat cgtcggcatt taattccagg gcatcgccaa agacgccaat
    18961 acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat caataacagc
    19021 gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcttgtg ccagacgttc
    19081 agcggcatct ttcgaccccg cataggtgaa tcgtacattg gccccatcgg tgacgaaacg
    19141 acgtacgata gcggcaccga ttccacgact gccaccgagg atgagaactg tcttaccggt
    19201 aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa actgttttaa
    19261 ctttctgtca acaggttttg taatggtcac taaaaaacaa tctcgcgttc caggtcgtcc
    19321 cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc accaaaaagg
    19381 tttcgatgca gtaagtgtct ctgaagttac tgattatctt ggtattaacc ccccgagtct
    19441 ctacgcggct tttggcagta aggctgggtt atttagccgt gtactcaatg aatatgtcgg
    19501 tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccggtag gcgagtgcct
    19561 ggttgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct gcgctggctg
    19621 tatggttctt gaaggtattc atagtcatga tccacaagcg cgtgatattg ccattcaata
    19681 ttatcacgcc gcagaaacga ccatttatga ctatatcgcc aggcggcatc cacaaagcgc
    19741 acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga aggcacggga
    19801 ggggcactcc atcgaacaac tctgtgcaac agctgccctg gcaggggaag cgataaaaac
    19861 tcttctcaag gagtgatgct ggccttgatc cgaaaggcgg gaacgcgcct gccgataagt
    19921 tgtgataaga caataattca cgcgttaagg ctggcggaat tgatcatctt ttcgtataac
    19981 gatagaaatg aaacattgtt ttaattaagg agtggaaaag atgatcatcg gaaatattca
    20041 taatcttcag ccgtggctac cccaggagtt acgccaggcg attgagcata tcaaagcaca
    20101 cgttacggca gaaacgccaa agggcaagca tgatatcgaa ggcaatcgtc tgttttatct
    20161 tatctcggaa gatatgaccg agccgtacga agctcgccgt gcggagtacc atgcccgcta
    20221 tcttgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca cgcaacctgc
    20281 aggcgcgccg gataccgact ggttagctga taaagacatc gcatttttgc cggaaggcgt
    20341 tgatgagaaa acagttatcc taaatgaagg tgattttgtt gtgttttatc cgggggaagt
    20401 gcataaaccg ctgtgcgcag tgggcgcgcc agcccaggtt cgcaaagcag tagtgaagat
    20461 gctgatggcg tgatgacttt tcgccgtaaa taactccagg tttacggcga gtttgtgaaa
    20521 agagcgtttt ttgatatttt tttgtgagta aaatttgtaa tgcttagacg ttcttattaa
    20581 ctcaaggagt tcgtcatggg caaaatatca gatttgaatt attctcaaca cattacatta
    20641 gccgacaatt ttaaacaaag aaatgaagcc ttagatacct ggtatgtggg aatgaatgat
    20701 tttgcccgaa ttgccggggg gcagaatagc agaagcaata ttctttctcc tagagcattt
    20761 ttggagtttt tggcaaagat atttaccctg ggttatgtgg attttagcaa acgctccaac
    20821 gaagcgggta gaaatatgat ggctcatatt gagttctcat cttattctaa agatactgat
    20881 ggcaatgaaa aaatgaagtt ttacatgaat aatcctgaag gggagcgagc ggatttatct
    20941 aaggtgaaga tagaaattac acttgcctca gcttctacta aagggattcg tgaaggacat
    21001 acagtcatta tatttaaaca atctgatggt tcgactaatc gttatgaagg gaagtctttt
    21061 gaaagaaaag atgatagttc attacacctg attactaaca aggttctggc gtgttaccaa
    21121 cgcgaagcta acaagaaaat agcgcgtcta ttaaataatc atcagaagtt aaataatata
    21181 caggagttaa atgattcgca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21241 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21301 acgattacgg atttattaga aaaagcattg aataa
//
LOCUS       DAJVPT010000068        21026 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVPT010000068 DAJVPT010000000
VERSION     DAJVPT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            8..781
                     /locus_tag="OQM47_002512"
                     /pseudo
     CDS             8..781
                     /locus_tag="OQM47_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(841..2139)
                     /gene="eno"
                     /locus_tag="OQM47_002513"
     CDS             complement(841..2139)
                     /gene="eno"
                     /locus_tag="OQM47_002513"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1480624.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2227..3864)
                     /gene="pyrG"
                     /locus_tag="OQM47_002514"
     CDS             complement(2227..3864)
                     /gene="pyrG"
                     /locus_tag="OQM47_002514"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1480625.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4092..4883)
                     /gene="mazG"
                     /locus_tag="OQM47_002515"
     CDS             complement(4092..4883)
                     /gene="mazG"
                     /locus_tag="OQM47_002515"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1480626.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4979..7213)
                     /locus_tag="OQM47_002516"
     CDS             complement(4979..7213)
                     /locus_tag="OQM47_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1480627.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7261..8562)
                     /gene="rlmD"
                     /locus_tag="OQM47_002517"
     CDS             complement(7261..8562)
                     /gene="rlmD"
                     /locus_tag="OQM47_002517"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1480628.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8619..11375
                     /gene="barA"
                     /locus_tag="OQM47_002518"
     CDS             8619..11375
                     /gene="barA"
                     /locus_tag="OQM47_002518"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1480629.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11607..12947)
                     /gene="gudD"
                     /locus_tag="OQM47_002519"
     CDS             complement(11607..12947)
                     /gene="gudD"
                     /locus_tag="OQM47_002519"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1480630.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12968..14308)
                     /locus_tag="OQM47_002520"
     CDS             complement(12968..14308)
                     /locus_tag="OQM47_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1480631.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14310..15662)
                     /gene="gudP"
                     /locus_tag="OQM47_002521"
     CDS             complement(14310..15662)
                     /gene="gudP"
                     /locus_tag="OQM47_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1480632.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16096..16545)
                     /locus_tag="OQM47_002522"
     CDS             complement(16096..16545)
                     /locus_tag="OQM47_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1480633.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16563..17345)
                     /gene="truC"
                     /locus_tag="OQM47_002523"
     CDS             complement(16563..17345)
                     /gene="truC"
                     /locus_tag="OQM47_002523"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1480634.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17345..17674)
                     /locus_tag="OQM47_002524"
     CDS             complement(17345..17674)
                     /locus_tag="OQM47_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1480635.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18296..18841)
                     /gene="syd"
                     /locus_tag="OQM47_002525"
     CDS             complement(18296..18841)
                     /gene="syd"
                     /locus_tag="OQM47_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1480636.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18909..19757
                     /gene="queF"
                     /locus_tag="OQM47_002526"
     CDS             18909..19757
                     /gene="queF"
                     /locus_tag="OQM47_002526"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1480637.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19869..>21026
                     /gene="ppnN"
                     /locus_tag="OQM47_002527"
                     /gene_synonym="ygdH"
     CDS             19869..>21026
                     /gene="ppnN"
                     /locus_tag="OQM47_002527"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1480638.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAA"
BASE COUNT         5177 a         5610 c         5010 g         5229 t
ORIGIN      
        1 accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta gcttaaactc
       61 acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata cccaagtgca
      121 aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat atgctacaag
      181 agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac tgatcgttgt
      241 tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag aaaaggtaac
      301 cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta aacaacaaaa
      361 attagctaag ggattagagt tagctataat cgctttgaat aaccaactca cttcacaaca
      421 ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc attattactt
      481 tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc atcaaggcag
      541 taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc ttttagattt
      601 attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt ttactttcac
      661 catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg gtagaagtta
      721 tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg gccgctggta
      781 actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat aaagtcagac
      841 ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc ccagagcttc
      901 ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca tagaaccagt
      961 tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat cttcagtttc
     1021 accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct tgattgcagc
     1081 cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt tagcgatacc
     1141 tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt caccaaccag
     1201 ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt cagattcgtc
     1261 cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca ggaagtgagt
     1321 gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt taccatcttt
     1381 gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca gttcataacc
     1441 agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac ccaggttcgg
     1501 cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca gaacttttgc
     1561 caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt tcgcgccaac
     1621 cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac caccgttgat
     1681 gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca gttcagcgat
     1741 gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag ccagagatac
     1801 agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca ggtcgatcat
     1861 gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct gagcgatcgg
     1921 gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg atttgtcgcc
     1981 atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag cagctgccat
     2041 accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac cacgggagtc
     2101 gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct caagtcacta
     2161 gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc gttctaactt
     2221 ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa agcctgcaaa
     2281 cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa
     2341 ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc
     2401 aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg
     2461 acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc tatcgtcaac
     2521 caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg
     2581 aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ccgggtactt
     2641 acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat ggcgagcgta
     2701 atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat tgttctcacg
     2761 cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac
     2821 gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc
     2881 gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcgat
     2941 cactgattta taagcatccg gcagttcaat gtacttgccg accataccga tggtgacttc
     3001 acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat ttgcttccgg
     3061 gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt tcaacaggcc
     3121 cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt
     3181 acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg aacgacaaat
     3241 caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt
     3301 ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagagt
     3361 gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg gcaaggattc
     3421 gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt cgccaccttc
     3481 cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg ttgcgccgag
     3541 gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt
     3601 gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt ccaagtcggt
     3661 ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg
     3721 atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag
     3781 aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga ccccgccggt
     3841 cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga
     3901 ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc
     3961 cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa
     4021 atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg gcgcttgacc
     4081 gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt
     4141 gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg
     4201 cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc tttcgtccct
     4261 aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc ctccagttta
     4321 gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc ttcgtagact
     4381 ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca acgtttctgg
     4441 attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc
     4501 tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag cacttcacta
     4561 ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa tttatcgcta
     4621 atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat ttgcgcgtaa
     4681 aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa atcttcacgg
     4741 gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat
     4801 gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg cataatagtg
     4861 agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg tcaggtgtaa
     4921 taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct atgtaagact
     4981 aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt ttacccagca
     5041 cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc gccagttgct
     5101 gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg gcgagaatgg
     5161 tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc accagcgaat
     5221 atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc gcatgggagc
     5281 gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt ccctgggtaa
     5341 tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg tggtgcatca
     5401 ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt tgcggtgtgt
     5461 agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca ctcggcttat
     5521 taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg ccaccaatcg
     5581 ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt ttttctgctt
     5641 ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc ccagccagaa
     5701 tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg
     5761 ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc tgtttctggg
     5821 tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca atgcgcccgc
     5881 caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa gcgaagtcca
     5941 gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag acatacaccc
     6001 ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca gccatctctt
     6061 cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc gaacgtgcgc
     6121 cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac tccgcatctt
     6181 catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc cccagaacca
     6241 cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg tcaaactcat
     6301 ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa tcctgtaaac
     6361 gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg aggttctttt
     6421 tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact tcagctttaa
     6481 cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata tagtgttcgc
     6541 ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc gggtggagat
     6601 aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga cggttcgcca
     6661 acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct tccggcgcat
     6721 cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg cagcgaaaat
     6781 catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag gaaacagaat
     6841 cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg tgaataaggt
     6901 taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg ttggcatcag
     6961 ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat gtcgagagga
     7021 tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc ccctgcgtct
     7081 gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg ctggtaatac
     7141 ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata tgtgcacttc
     7201 ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg caaatcgtaa
     7261 ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc
     7321 cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc tatcccgagc
     7381 cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt ttataatttg
     7441 ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat cgaagccgtt
     7501 tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa acgtcacatt
     7561 ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag gaacaccttc
     7621 tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc ccataccgca
     7681 gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg cacgcgctac
     7741 cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa aagttaagcg
     7801 caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta tctcactatc
     7861 gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt tttcgcgatc
     7921 tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg ttgcctgtac
     7981 cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg ccctgacttt
     8041 gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt cgacaatgtc
     8101 actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta agtagttcag
     8161 acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt cagagacatc
     8221 gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat ccacgctggc
     8281 gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg tttcgcgttc
     8341 cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt tatcttcagt
     8401 aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg ttttgccgtt
     8461 atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg ttatgatctg
     8521 acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga ctttctcaat
     8581 ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac agcctgcgcg
     8641 cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg ctgagtatct
     8701 ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc ggtgctagca
     8761 ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat cgcgaatcta
     8821 tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc gcgatttcgg
     8881 tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat ccctcatcaa
     8941 tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt gacggcgata
     9001 ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa tcgcccagta
     9061 gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg gatcttaaat
     9121 cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg ctgttttgta
     9181 tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt ccgattcgca
     9241 acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg gtggaaggat
     9301 ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg
     9361 ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat ctgcgtgaaa
     9421 cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag cgcgcccagg
     9481 aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg cgtacaccac
     9541 tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca ccaacgcagc
     9601 gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt attaatgatg
     9661 ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt ccattcccac
     9721 tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac gataaagggt
     9781 tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc gacccactgc
     9841 gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc gagaatggca
     9901 acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag attgaagtgc
     9961 agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta ttccaggcct
    10021 tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg gggctggtga
    10081 ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc cagccgaatc
    10141 gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt attatcgaag
    10201 ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca aactccgcag
    10261 cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt tatagcccaa
    10321 cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc gcggtgacct
    10381 tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg atgaccgatt
    10441 tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc aagcaagatg
    10501 gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct gccctgacgg
    10561 aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt aagctggcaa
    10621 tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc gcattgctgg
    10681 aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt gaacgggcga
    10741 aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg gatggcattc
    10801 gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt atcgcggtaa
    10861 cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg agcgattatc
    10921 tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac aagcctggca
    10981 gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg aacccgaatg
    11041 cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg
    11101 atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt gaggaacagc
    11161 tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat ggcagttgcg
    11221 gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa cagctacgta
    11281 gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac gagatggata
    11341 atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga gtagcaatcg
    11401 gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg ccggatgcgg
    11461 cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa aaatgatgta
    11521 gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa accggctcca
    11581 gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt gttatcgaac
    11641 gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc aagcccgtgt
    11701 ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac acccagcccc
    11761 ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt ggtcaggcgc
    11821 tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc cggtgcagcg
    11881 gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga gtgtgaaccc
    11941 caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg catagtccag
    12001 aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg gcccatctgc
    12061 cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg acggaactct
    12121 gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc tgcataagcc
    12181 agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca ggcaccgtta
    12241 ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat agactcggct
    12301 tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc atatttttca
    12361 tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc atgacgcagg
    12421 cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt ggctttgcga
    12481 ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg accatcgccc
    12541 agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat tgccgcttct
    12601 atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc gcgcccacta
    12661 gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta ttcacccagc
    12721 gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt ctcgccgccg
    12781 ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat attacgcgtg
    12841 aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc cgccaccggg
    12901 atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt tatgtccttt
    12961 ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag ccggggatca
    13021 ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta taggcctcat
    13081 gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca tcaggaacgg
    13141 caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg ccctcctgcc
    13201 aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc acatgggtaa
    13261 atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc agcccccagt
    13321 cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc ggatcggcaa
    13381 gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag ttggtggcga
    13441 tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc acttcacgtc
    13501 cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca tcattcagcc
    13561 ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca acggtaatcc
    13621 gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc tcgccaggta
    13681 acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag gcttccgcca
    13741 gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac cactcatggt
    13801 tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct atataaaaca
    13861 gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt tcgcagaccg
    13921 gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc gccacggcat
    13981 taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg gctgcctgat
    14041 tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg cccagaacca
    14101 tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct tcaccaatgc
    14161 cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat gcgttatgcg
    14221 caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact ttcatatcag
    14281 taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt cagctcgata
    14341 cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc atgaacacca
    14401 acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc aattgcgatt
    14461 ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga aagaccgctg
    14521 atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat gcccttaccg
    14581 aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta gttgcagaac
    14641 accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc aatgttcagc
    14701 gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc gccgataaaa
    14761 ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat cccacgcgcc
    14821 tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt gatacagtac
    14881 tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat ctgcccccac
    14941 ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat cagcgcgcca
    15001 cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt tggctcgtgg
    15061 atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa gaagaagacg
    15121 tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat caccgttgcg
    15181 aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac cggaaaccag
    15241 gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc gaccaggaag
    15301 cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac gaagccttgc
    15361 agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt tgaaccaaaa
    15421 cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga gaaggcagag
    15481 aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc cgaaccggca
    15541 atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa catcaccact
    15601 atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact taaagaactc
    15661 attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg acatcgccag
    15721 gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg ccgaatacgg
    15781 cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct caccgtgcaa
    15841 caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg gacggtggaa
    15901 tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga agaaataaag
    15961 aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc gcgctatgaa
    16021 gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa acgacattta
    16081 ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg gatttgattc
    16141 cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa cccgctgagc
    16201 gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat ttacataact
    16261 gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca gactatcttt
    16321 gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg tcgtggacgt
    16381 aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag gatcttcaaa
    16441 taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg ccactaacag
    16501 cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt tttctccctg
    16561 gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac cctttcattt
    16621 tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt gtcgtccagg
    16681 cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa ctgactggca
    16741 tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg gcgtaaatcg
    16801 ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg tcgaagctga
    16861 tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt cgggtaacgt
    16921 ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac tgctggctgc
    16981 gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc caccagtgga
    17041 taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta acgtttctgg
    17101 atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga taatcccatc
    17161 aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg ctgtcctatc
    17221 tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca gctacggtga
    17281 accagccagc cggagggttt atttaccgca accagccatt catcctgata gagtatttcc
    17341 agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca gccagaataa
    17401 gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca acagcaaaag
    17461 cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc agcccccact
    17521 gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta ttaaattgat
    17581 gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct tcaagcgcct
    17641 gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa gaaaaactgc
    17701 cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc ccggttcgtt
    17761 tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt ttcctctggc
    17821 cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc cgatgtgata
    17881 cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac cggttctcat
    17941 tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct tcatccagaa
    18001 gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc ctgatgttca
    18061 cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga agtcttacaa
    18121 ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat taacaatcaa
    18181 cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc ttatttcctg
    18241 gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat ttggattaca
    18301 gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc gtccgtttac
    18361 gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag atgacctcta
    18421 actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt ttctgagtca
    18481 ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc caggtttgca
    18541 gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac tgagtcgtat
    18601 aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc gcgtttacat
    18661 tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta gttgaaataa
    18721 tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta tgctcttcat
    18781 gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc aaatcgtcca
    18841 ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct ggtttatgac
    18901 ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact cttggaaaat
    18961 caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca cgcagcctga
    19021 atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg gatatctgga
    19081 cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct gtcggtcatg
    19141 ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag ctctatctca
    19201 acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg ctggagcgcg
    19261 acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt gatgaactgg
    19321 aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat atcactatcg
    19381 ataactatga attcactact gactatctgg agaatgccac cagcggtgaa aaagtagtgg
    19441 aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat caaccagatt
    19501 ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa ctgctgcgtt
    19561 acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa cgcatcttta
    19621 atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt tatacccgtc
    19681 gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca tcgaccacaa
    19741 gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc agaaggttgt
    19801 gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg tcccataagg
    19861 agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg tcgcagctgg
    19921 aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt cgcaactgtt
    19981 cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg ctgtctcgtt
    20041 ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg gaactgatta
    20101 atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag gccaacttgt
    20161 tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc gttcgttttc
    20221 ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc atcttgcgta
    20281 acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg ggcggtcact
    20341 caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg ggcctgcgtg
    20401 agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg aaaggtgctg
    20461 cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg acagagccgt
    20521 cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc atgccggata
    20581 tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc ttccctggcg
    20641 gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac ccggccaaca
    20701 aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac tacttccgcg
    20761 tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat taccgcatca
    20821 tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg ctggtgaaag
    20881 aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc attgcgccag
    20941 atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag ctttacccgg
    21001 atcaacctgt tgaagtgctg gctgcc
//
LOCUS       DAJVPT010000069        20566 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVPT010000069 DAJVPT010000000
VERSION     DAJVPT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1499)
                     /gene="glyS"
                     /locus_tag="OQM47_002528"
     CDS             complement(<1..1499)
                     /gene="glyS"
                     /locus_tag="OQM47_002528"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1480639.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDL"
     gene            complement(1509..2420)
                     /gene="glyQ"
                     /locus_tag="OQM47_002529"
     CDS             complement(1509..2420)
                     /gene="glyQ"
                     /locus_tag="OQM47_002529"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1480640.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(2515..2814)
                     /locus_tag="OQM47_002530"
     CDS             complement(2515..2814)
                     /locus_tag="OQM47_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1480641.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2989..3984
                     /gene="wecH"
                     /locus_tag="OQM47_002531"
     CDS             2989..3984
                     /gene="wecH"
                     /locus_tag="OQM47_002531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1480642.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4026..4463)
                     /locus_tag="OQM47_002532"
     CDS             complement(4026..4463)
                     /locus_tag="OQM47_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1480643.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4509..4850)
                     /locus_tag="OQM47_002533"
     CDS             complement(4509..4850)
                     /locus_tag="OQM47_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480644.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5019..6473)
                     /gene="xylB"
                     /locus_tag="OQM47_002534"
     CDS             complement(5019..6473)
                     /gene="xylB"
                     /locus_tag="OQM47_002534"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1480645.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(6545..7867)
                     /gene="xylA"
                     /locus_tag="OQM47_002535"
                     /pseudo
     CDS             complement(6545..7867)
                     /gene="xylA"
                     /locus_tag="OQM47_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8233..9225
                     /gene="xylF"
                     /locus_tag="OQM47_002536"
     CDS             8233..9225
                     /gene="xylF"
                     /locus_tag="OQM47_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1480646.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9303..10844
                     /gene="xylG"
                     /locus_tag="OQM47_002537"
     CDS             9303..10844
                     /gene="xylG"
                     /locus_tag="OQM47_002537"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1480647.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            10822..12003
                     /gene="xylH"
                     /locus_tag="OQM47_002538"
     CDS             10822..12003
                     /gene="xylH"
                     /locus_tag="OQM47_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1480648.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12081..12611
                     /locus_tag="OQM47_002539"
     CDS             12081..12611
                     /locus_tag="OQM47_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1480649.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            12723..13259
                     /locus_tag="OQM47_002540"
     CDS             12723..13259
                     /locus_tag="OQM47_002540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1480650.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13367..14191)
                     /locus_tag="OQM47_002541"
     CDS             complement(13367..14191)
                     /locus_tag="OQM47_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1480651.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            14511..16540
                     /gene="malS"
                     /locus_tag="OQM47_002542"
                     /pseudo
     CDS             14511..16540
                     /gene="malS"
                     /locus_tag="OQM47_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            16718..17971
                     /gene="avtA"
                     /locus_tag="OQM47_002543"
     CDS             16718..17971
                     /gene="avtA"
                     /locus_tag="OQM47_002543"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1480652.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18122..18595)
                     /locus_tag="OQM47_002544"
     CDS             complement(18122..18595)
                     /locus_tag="OQM47_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1480653.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPAACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(18697..19545)
                     /gene="yiaJ"
                     /locus_tag="OQM47_002545"
     CDS             complement(18697..19545)
                     /gene="yiaJ"
                     /locus_tag="OQM47_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1480654.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            19745..>20566
                     /gene="yiaK"
                     /locus_tag="OQM47_002546"
     CDS             19745..>20566
                     /gene="yiaK"
                     /locus_tag="OQM47_002546"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1480655.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT"
BASE COUNT         5179 a         5217 c         5048 g         5122 t
ORIGIN      
        1 agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc ggcacgacgc
       61 agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat acccgccaga
      121 gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg caggtcatca
      181 ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc cgcttcgcca
      241 tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc gaagaccatg
      301 ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt aacgtcagcg
      361 ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt gtcacgcagc
      421 gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc ttccagacgt
      481 tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg aacgactttc
      541 tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac gaagataaag
      601 ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc acctttcatg
      661 gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt tgcggtcaga
      721 acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct taagtcagcg
      781 ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc tttacgttct
      841 tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg atcggcgttg
      901 tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg atcggactga
      961 atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac ggtgtgcacc
     1021 ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat cggcagtttc
     1081 gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc cttcacatgg
     1141 gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg gtcaacggta
     1201 ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc agcgtcgaac
     1261 gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc ttccgccagg
     1321 ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt gccgtgtgcg
     1381 aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc caggctgcgc
     1441 agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt ctcagacata
     1501 gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg ggaagcgtaa
     1561 tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg ctgacgctcg
     1621 gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc tttcagaata
     1681 cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg cgcttctttc
     1741 tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa gttgtaagtg
     1801 gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc cagcgggccg
     1861 tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc cagacgttcc
     1921 agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac ctgctggaag
     1981 taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag tccccaggcc
     2041 cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat agtcgggtcc
     2101 atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg cgatggctta
     2161 atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc gtagcgacca
     2221 tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg ccccagcgcg
     2281 cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa tggttgaaca
     2341 atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc ctggaaggtc
     2401 ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta aaggaaggga
     2461 tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca gggcttaacg
     2521 catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact gctctttacg
     2581 gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg tgacgtcgat
     2641 tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg cttccatatt
     2701 caacgaacgc agaggatcaa cccttacccg ttgtgctttc tgcacgggtg acggtgtaga
     2761 acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca tcattaaatt
     2821 accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg atatcttacg
     2881 aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca gtgcgccaac
     2941 caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat gcagcccaaa
     3001 atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat gattcacacc
     3061 actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga tatcgccaat
     3121 gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc cggctatctc
     3181 ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt atgtctgatt
     3241 ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa tatggagtta
     3301 gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt cttcgcgatt
     3361 gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg aaaaatgttg
     3421 ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc gcagaaaatt
     3481 gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt ttactacatt
     3541 ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa agcactgtcg
     3601 tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg gacattatat
     3661 gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg gccgatggtt
     3721 tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac gcgtaccatt
     3781 cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca cgccttgatt
     3841 atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact ggatattatt
     3901 tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt acaacgaatc
     3961 gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga ccgggcttac
     4021 gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg gtttattccg
     4081 gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa gaaagccagt
     4141 ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac catcagtaga
     4201 gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt ggtcggtatg
     4261 ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag tcctaacacc
     4321 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
     4381 aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
     4441 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
     4501 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
     4561 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
     4621 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
     4681 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
     4741 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
     4801 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
     4861 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa aatgccatta
     4921 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
     4981 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
     5041 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
     5101 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
     5161 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
     5221 gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta
     5281 cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca
     5341 tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg
     5401 gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt
     5461 gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt
     5521 tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc
     5581 agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg
     5641 caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag
     5701 acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact
     5761 gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct
     5821 ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc
     5881 tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca
     5941 tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc
     6001 agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca
     6061 tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta
     6121 atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg
     6181 tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca
     6241 tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga
     6301 gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccaaagt
     6361 ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg
     6421 ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc
     6481 gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta
     6541 gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac
     6601 tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca
     6661 tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga
     6721 tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca
     6781 tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac
     6841 gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat
     6901 acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt
     6961 gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta
     7021 tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa
     7081 tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat
     7141 attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc tagaagccga
     7201 ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac
     7261 gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt
     7321 caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga
     7381 agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt
     7441 tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa
     7501 ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca
     7561 catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg
     7621 ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc
     7681 ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac
     7741 gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg
     7801 ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg
     7861 cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt
     7921 gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag ataattcaca
     7981 agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaaa tgggtaattc ctcgaagata
     8041 aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata
     8101 aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta
     8161 aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga
     8221 aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt
     8281 accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt
     8341 cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggtgcg
     8401 aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac
     8461 atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt
     8521 aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt
     8581 aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag
     8641 gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg
     8701 gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt
     8761 gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac
     8821 gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt
     8881 gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca
     8941 gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc
     9001 ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac tgccgcagaa
     9061 attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact gaataatggc
     9121 ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa
     9181 gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc
     9241 gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg
     9301 ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga
     9361 ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg
     9421 ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg
     9481 aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac
     9541 gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg
     9601 aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga
     9661 cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg
     9721 ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac
     9781 aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg
     9841 ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc
     9901 acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc
     9961 acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg
    10021 agcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat gaaatattac
    10081 gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg
    10141 tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac
    10201 gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt
    10261 atattgatgg caaacaggta gatattcgta actgtcagca agccatcacc caggggattg
    10321 cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa
    10381 atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg
    10441 cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg
    10501 accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc gcccgctgtc
    10561 tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt gatattggcg
    10621 cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg
    10681 tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg
    10741 aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag
    10801 ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta
    10861 ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt
    10921 gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc
    10981 gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga
    11041 atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta
    11101 ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc
    11161 attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac
    11221 cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc
    11281 attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc acaaattggg
    11341 caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt aatggctttt
    11401 gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca gtctccggcc
    11461 tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg cgcaatctgg
    11521 ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc
    11581 ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc
    11641 aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc
    11701 gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct
    11761 ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc
    11821 ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc
    11881 atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc
    11941 gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct
    12001 tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat
    12061 gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg
    12121 ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcatcac
    12181 aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg
    12241 actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg
    12301 ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc
    12361 cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt
    12421 taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac
    12481 gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg
    12541 gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc
    12601 tggcagactg actacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc
    12661 gagcgcggca tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat
    12721 gcgtgattgg catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt
    12781 cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat
    12841 tagataaaga agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac
    12901 ggcgctcaac agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca
    12961 ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct
    13021 cgcgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgcaatga
    13081 ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca
    13141 atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa
    13201 aagcatatga cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc
    13261 gatgatgaga attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa
    13321 aagccttatc cgaccgacaa atcagataag gctcgaggaa atgaaattac atatgcgccg
    13381 cgattaaccg ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt
    13441 tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt
    13501 tacgcagctg tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag
    13561 tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac
    13621 ccggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc
    13681 gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg
    13741 tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag
    13801 accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgccc
    13861 attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt
    13921 ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag
    13981 ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt
    14041 tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg
    14101 tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg
    14161 ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc
    14221 aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg
    14281 tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga
    14341 ttggcgctaa atcgcagaaa atgtgggggt tatcgcaaaa ttcagccgtt ttttgcgcgc
    14401 gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg
    14461 cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg
    14521 ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg
    14581 ggttccctgc ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca
    14641 aaggtacgcg tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga
    14701 taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat
    14761 tgttcaggga cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga
    14821 tgatttccat ccagaacgcg ccgaaccggt agcaaacctg gtccgtgaat gcccgaaatg
    14881 ggatggatta ccgctcacgc tggatgtcag cgccactttc ccggaaggag ccgccgtacg
    14941 ggattattac agccaacaaa ttgcgatagt gaagaacggt caaataacgt tacaacccgc
    15001 tgctaccagc aacggtttac tcctgctgga acgggcagaa actgacacat ccgccccttt
    15061 cgactggcat aacgccacgg tttactttgt gctgacagat cgtttcgaaa acggcgatcc
    15121 cagtaatgac cagagttacg gacgtcataa agacggtatg gcggaaattg gcacttttca
    15181 cggcggcgat ttacgcggcc tgaccaacaa actggattac ctccagcagt tgggcgttaa
    15241 tgctttatgg ataagcgccc catttgagca aattcacggc tgggtcggcg gtggtacaaa
    15301 aggcgatttc ccgcattatg cctaccacgg ttattacaca caggactgga cgaatcttga
    15361 tgccaatatg ggcaacgaag ccgatctacg gacgctggtt gatagcgcac atcagcgcgg
    15421 tattcgtatt ctctttgatg tcgtgatgaa ccacaccagc tatgccacgc tggcggatat
    15481 gcaggagtat cagtttggcg cgttatatct ttctggtgac gaagtgaaaa aaacgctggg
    15541 tgaacgctgg agcgactgga aacctgccgc cggacaaacc tggcatagct ttaacgatta
    15601 cattaatttc agcgacaaaa caggctggga taaatggtgg ggaaaaaact ggatcagaac
    15661 ggatatcggc gattacgaca atcctggatt cgacgatctc actatgtcgc tggccttttt
    15721 gccggatatc aaaaccgaat caactaccgc ttctggtctg ccggtgttct ataaaaacaa
    15781 aacggatact cacgctaaag tcatcgaagg ctttacacct cgcgattact taacccactg
    15841 gttaagtcag tgggtccgcg actatgggat tgatggtttt cgggtcgata ccgccaaaca
    15901 tgttgagttg cccgcctggc agcaactgaa aaccgaagcc agctccgcgc ttcgcgaatg
    15961 gaaaaaaact aaccccgaca aagcattaga tgacaaacct ttctggatga ccggtgaagc
    16021 ctggggccac ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg cgatgatcaa
    16081 tttcgattat caggagcagg cggcgaaagc agtcgattgt ctggcgcaga tggatacgac
    16141 ctggcagcaa atggcggaga aattgcaggg tttcaacgtg ttgagctacc tctcgtcgca
    16201 tgatacccgt ctgttccgtg aagggggcga caaagcagca gagttattac tattagcgcc
    16261 aggcgcggta caaatctttt atggcgatga atcctcgcgt ccgttcggtc ctacaggttc
    16321 tgatccgctg caaggtacac gttcggatat gaactggcag gatgttagcg gtaaatctgc
    16381 cgccaacgtc gcgcactggc agaaaatcag ccagttccgc gcccgccatc ccgcaattgg
    16441 cgcgggcaaa caaacgacac tttcgctgaa gcagggctac ggctttgttc gtgagcatgg
    16501 cgacgataaa gtgctggtca tctgggctgg gcaacagtga cttttccggc ttcccgttcg
    16561 tcagtacctc gggaagccgc caaccaggat aaaatgtcag ccctaatcag cgttgcggga
    16621 taaagcaccg ctcactcttc aacagaccga tttgcacccc agcaaatgta gcgttattgt
    16681 taccttcctt gctacagagt tcgacagata tcccgctatg acattctccc tttttggtga
    16741 caaatttacc cgccactccg gcattacgct gctgatggaa gatctgaacg acggtttacg
    16801 cacgcctggc gcgattatgc tcggcggcgg taatccggcg cagatcccgg aaatgcagga
    16861 ctacttccag acgctactga ccgacatgct ggaaagtggc aaagcgactg atgcactgtg
    16921 taactacgac ggtccacagg ggaaaacgga gctactcaca ctgcttgccg gaatgctgcg
    16981 cgagaagttg ggttgggata tcgaaccaca gaatattgca ctaacaaacg gcagccagag
    17041 cgcgtttttc tacttattta acctgtttgc cggacgccgt gccgatggtc gggtcaaaaa
    17101 agtgctgttc ccgctggcac cggaatacat tggctacgct gacgccggac tggaagaaga
    17161 tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt ttaaatacca
    17221 cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg tctcccggcc
    17281 gacgaatcca acaggtaatg tgattactga cgaagagttg ctgaagcttg acgcgctggc
    17341 gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt tcccgggtat
    17401 catcttcagt gaagggcgcc cgctatggaa tccgaatatc gtactgtgca tgagtctttc
    17461 caagctgggg ctgcctggct cccgctgcgg cattatcatc gccaatgaaa aaatcatcac
    17521 tgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg gtccggcgat
    17581 gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag tcatcaaacc
    17641 gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt taccggaaaa
    17701 tcgctgcctg attcataaac cggaaggagc cattttcctc tggctatggt ttaaggattt
    17761 gcccattacg accgagcagc tctatcagcg cctgaaagca cgcggcgtgc tgatggtgcc
    17821 ggggcacaac ttcttcccgg gactggataa accgtggccg catacgcatc aatgtatgcg
    17881 catgaactac gtaccagagc cggagaaaat tgaggcgggg gtgaagattc tggcggaaga
    17941 gatagaaaga gcctgggctg aaagtcacta aaaattgcct gatgcgctac gcttatcagg
    18001 cctacatgat ttctgcaata tattgaattt ggaagaattt gtaggccgga taaggcgttt
    18061 acgccgcatc tggcataaac aaagcgcact attccggcgt tgagaaacgc cggaaaacgt
    18121 ttcaaacagg ctgctgctgt agccgatgcc gctgcacttt ctcgacatcc atgcactgca
    18181 acgcctttgt cgggcaggct tcaacacagg ccggccccgt ctcccgatgc cagcacagat
    18241 cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac ctccatcgca ccaaacgggc
    18301 aagccagcat acagctttta cagccaatgc aacgtgtttg ttcaacgaaa atatgcccat
    18361 gttcgcggct tatcgcgtca acagggcaga cattcgcgca cggtgcatct tcacactgat
    18421 gacaggctgc tggcgtggtc cagctgtggt ctttaatgac acgaatacgg gaaataaact
    18481 cgtctggtga caacgcagcg caatcctgat tctcctgatg cgacactgcg caagccactt
    18541 cacaggtacg gcaaccgata catttcgtcg catccgcaat aataaaccgg ttcatgatca
    18601 ttcctttgca ctaaaacagc agagtgccag agagcgcgtc cctactgctt tgatccaacg
    18661 aaggaaatgt taaagttttg gcacttgtcc aaagcgttat gtgatcgcgc ccaggtcatc
    18721 cctgacagta aatcccagtt cattagaaat agcctgcgcg gtttcacgca gtggtttcag
    18781 gagatttttc tctcccacct gtttcagacg tgatgtcgaa agcgaaatcg acacggcgta
    18841 cggcacccgc ccatgaatat caaacaccgg aacagcaata caggagacgc cgagttcgtt
    18901 ttcttctctg tccatcgccg ctccgctttc acgaatgtgc gccagttcgt cgaacatcgc
    18961 gggcagctcg gtaatggtat tgcgggttaa cggctggatc tcatgctgat ggttttccca
    19021 gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag atcttgccca ttgcggaaca
    19081 gtagagcggc atatgctggc caatataggc acgggttcgc agcatcccgg ttgtgggttc
    19141 cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg gagaagttaa tggtttcacc
    19201 agtggcgatg ttcagtgcct caagatgcgg agcggcgata tgaatgatat tcagcgaaga
    19261 cagcgccttc tgcccgacgg caataaattt ggtagtcagg cggtaactcc ctgcggcggg
    19321 cgcggtggtc acatagccgc aggactgtaa tccctgcaat aagcgatgga cggtactctt
    19381 atttaaacca gccagctccg aaagatgcgc caacggacaa ccgtttggat agttgctcaa
    19441 aatctcaatc agcatcaacc cgcgaaacag actctggctt ccggctggac gctctttttc
    19501 ctgcgccatc tcgttctctt tttttcccat cacttctttc cccattttgt cgcgtcctga
    19561 tggtagcgca aagtgtgccg tagttcacga tctcgacaga taatttataa ccacttgatt
    19621 tttatgtctt ttgaaattca tcaatcagat tgctttgtta aaaagtgatc gatatatttg
    19681 aaatctagtt ccgcatattg aaattttaag ccaaaaaagc gatcaaaaaa caaggaagcc
    19741 taggatgaaa gtgacatttg agcagttaaa agcagccttt aatcgggtct taatttcacg
    19801 cggcgttgac agcgaaacgg ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc
    19861 cggcgtttat tctcacggcg ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg
    19921 cgatatcatt cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg
    19981 ggatgcccag cgttcgatcg gcaacctgac ggcgaaaaag atgatggatc gcgccattga
    20041 actggctgcc gatcacggta ttggtctggt ggcacttcgt aatgccaacc actggatgcg
    20101 cggcggcagc tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa
    20161 ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt cgcatcggca ctaacccgct
    20221 gatcgtcgcc attccttcca cgccgatcac catggtcgat atgtcgatgt tctcttacgg
    20281 catgttagaa gttaaccgcc tggcaggccg tcagctcccg gtcgatggtg gctttgatga
    20341 tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat
    20401 gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc
    20461 cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca
    20521 aatttttatt gccattgaag tggacaagct tatcgacggt cccacc
//
LOCUS       DAJVPT010000070        20555 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVPT010000070 DAJVPT010000000
VERSION     DAJVPT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            39..338
                     /locus_tag="OQM47_002547"
     CDS             39..338
                     /locus_tag="OQM47_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1480656.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            348..872
                     /gene="ygaP"
                     /locus_tag="OQM47_002548"
     CDS             348..872
                     /gene="ygaP"
                     /locus_tag="OQM47_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1480657.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(919..1323)
                     /gene="stpA"
                     /locus_tag="OQM47_002549"
     CDS             complement(919..1323)
                     /gene="stpA"
                     /locus_tag="OQM47_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1480658.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1991..2440
                     /gene="alaE"
                     /locus_tag="OQM47_002550"
     CDS             1991..2440
                     /gene="alaE"
                     /locus_tag="OQM47_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1480659.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2477..2821)
                     /locus_tag="OQM47_002551"
     CDS             complement(2477..2821)
                     /locus_tag="OQM47_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1480660.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2973..3203
                     /locus_tag="OQM47_002552"
     CDS             2973..3203
                     /locus_tag="OQM47_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480661.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3550..3795
                     /gene="nrdH"
                     /locus_tag="OQM47_002553"
     CDS             3550..3795
                     /gene="nrdH"
                     /locus_tag="OQM47_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1480662.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3792..4202
                     /gene="nrdI"
                     /locus_tag="OQM47_002554"
     CDS             3792..4202
                     /gene="nrdI"
                     /locus_tag="OQM47_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1480663.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4175..6319
                     /gene="nrdE"
                     /locus_tag="OQM47_002555"
     CDS             4175..6319
                     /gene="nrdE"
                     /locus_tag="OQM47_002555"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1480664.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6329..7288
                     /gene="nrdF"
                     /locus_tag="OQM47_002556"
     CDS             6329..7288
                     /gene="nrdF"
                     /locus_tag="OQM47_002556"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1480665.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7643..8845
                     /gene="proV"
                     /locus_tag="OQM47_002557"
     CDS             7643..8845
                     /gene="proV"
                     /locus_tag="OQM47_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1480666.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8838..9902
                     /gene="proW"
                     /locus_tag="OQM47_002558"
     CDS             8838..9902
                     /gene="proW"
                     /locus_tag="OQM47_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1480667.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9960..10952
                     /gene="proX"
                     /locus_tag="OQM47_002559"
     CDS             9960..10952
                     /gene="proX"
                     /locus_tag="OQM47_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1480668.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11144..12323
                     /locus_tag="OQM47_002560"
                     /pseudo
     CDS             11144..12323
                     /locus_tag="OQM47_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12447..13184
                     /gene="ygaZ"
                     /locus_tag="OQM47_002561"
     CDS             12447..13184
                     /gene="ygaZ"
                     /locus_tag="OQM47_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1480669.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13174..13509
                     /gene="ygaH"
                     /locus_tag="OQM47_002562"
     CDS             13174..13509
                     /gene="ygaH"
                     /locus_tag="OQM47_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1480670.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13600..14130
                     /gene="emrR"
                     /locus_tag="OQM47_002563"
     CDS             13600..14130
                     /gene="emrR"
                     /locus_tag="OQM47_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1480671.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14257..15429
                     /gene="emrA"
                     /locus_tag="OQM47_002564"
                     /pseudo
     CDS             14257..15429
                     /gene="emrA"
                     /locus_tag="OQM47_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15446..16984
                     /gene="emrB"
                     /locus_tag="OQM47_002565"
     CDS             15446..16984
                     /gene="emrB"
                     /locus_tag="OQM47_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1480672.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17048..17563)
                     /gene="luxS"
                     /locus_tag="OQM47_002566"
     CDS             complement(17048..17563)
                     /gene="luxS"
                     /locus_tag="OQM47_002566"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1480673.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17713..19269)
                     /gene="gshA"
                     /locus_tag="OQM47_002567"
     CDS             complement(17713..19269)
                     /gene="gshA"
                     /locus_tag="OQM47_002567"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1480674.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19342..19770)
                     /locus_tag="OQM47_002568"
     CDS             complement(19342..19770)
                     /locus_tag="OQM47_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1480675.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19767..20333)
                     /gene="yqaB"
                     /locus_tag="OQM47_002569"
     CDS             complement(19767..20333)
                     /gene="yqaB"
                     /locus_tag="OQM47_002569"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1480676.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4963 a         5262 c         5136 g         5194 t
ORIGIN      
        1 tttagagttt acttaattta gaaaatactt aaataaatat gactgaactc gcgcaattac
       61 aggccagtac cgaacaggca gcggccttat tgaaagcaat gagccaccct aaacggttgc
      121 tgattctgtg catgcttagc ggttcccccg gcaccagcgc gggagagctg acgcgcatta
      181 ccggactgag cgcctctgcg acatcacagc atctcgctcg tatgcgggac gaagggctta
      241 tcaacagcca acgggatgcc caacgcattc tatattccat taaaaatgag gcggtaaatg
      301 ccattatcgc caccctgaaa aatgtctatt gtccgtaagg agtcactatg gctttgacaa
      361 ccatttcgcc gcatgatgca caagaattaa tcgcacgcgg cgcaaagtta atcgatattc
      421 gtgacgctga tgaatatctt cgtgaacata ttcctgaagc agatctggct ccattatccg
      481 tgctggaaca gtcaggtctt ccacctaaat tacgtcgcga gcaaattata ttccactgcc
      541 aggcaggtaa acgcaccagt aataacgccg ataaattagc agcgattgcc gcccccgcag
      601 aaatattttt actcgaagat gggattgatg gctggaaaag agctggattg ccagtagcgg
      661 taaataaatc tcaacccttg ccgttaatgc gccaggtgca gatcgctgcg ggtggtttaa
      721 tattaaacgg cgttgtactg ggttataccg taaatagcgg tttcttctta ttaagtggct
      781 ttgttggtgc cgggttactg tttgcaggaa tcagcggttt ttgtggaatg gcaaggttgt
      841 tagataagat gccgtggaac caacgagctt gagaagcgac gccggacgcg ccctagcagc
      901 gacatccggc ctcagtaatt agatcaggaa atcgtcgaga gatttacctt ctgccagcgc
      961 ctgagcaatt ggcttcggtg tacggccctg accggtccag gttttagttt caccgttaac
     1021 atcagtgaat ttatatttcg ccggacgcgg ctggcgtttt ttaccagcac gtggtgcagc
     1081 agcagagcta ttacccaata actcttccgg gttaattccg tcagctttca tcagctccag
     1141 ccaggtgcta attttttcct gacgctcagc cagttcacgc tgctgctgtt cttcttcttc
     1201 acgtctttct ttagtgacaa ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga
     1261 gaattcgcga gccatcgcac ggagggtgcg aatgttattt aaactttgta acattacgga
     1321 cataataaag aaaacctttt aacgccaaaa caaaaaagta tttcgtcgcg tagtgtaagc
     1381 gatcacttat tatatttcca caaaatttaa tctatttaca gtcctgcgcg agattatttc
     1441 agctgttatt ttctgaaact ctcagaataa tttcaagatt atatattagc cattttgatg
     1501 agtaatttag gttattggtg aacagagtgg cgctctctgc tcgctaactc cgcaagcctg
     1561 acaaaaccga agtgaataaa aatacaatat aacagaataa aagaaaacac ccctcactgg
     1621 tgctttctgc tgttaatgta cattttctgg catcaaaaat gctgtaaatg acccggtcat
     1681 gacaaacacc gaattctcta cgcttatagc gttacctccc ccccaaactt tcattagtga
     1741 aattaaaggc aagatcatat ctaattgatt taattgaaaa taaatcatat tggcatgtaa
     1801 aaatccacaa tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactaacta
     1861 aataaaaatt tttcactaat tgattagtca taggcagcga tatacgctat gcaaaaatgc
     1921 agatggcaat gagatccact gctttcatct ccattaacat cccattacgc ttttattaag
     1981 gagcattagc atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc
     2041 gatggttgtt tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag
     2101 cttcgaacag tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg
     2161 gccatacggt atgtaccgcg atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg
     2221 ctggataaaa aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt
     2281 ggcgatcttg ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa
     2341 catcgttgtt tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg
     2401 actgtttaaa gtcagccgtt accagcaggt aaaagcctga ctcttctttt cgcgactggc
     2461 atcgccagtc gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg
     2521 caacacgcga actaaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat
     2581 agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg
     2641 ttgaactgcg ttcttttaat gttggatgaa ttaccagagc ggtacgtccc attcgcgctt
     2701 cgcgattaac atagacataa ttttccccac ggcggtaacc atacgctttc tgggttacca
     2761 catcgacagt aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca
     2821 tatttgatcc tcgttatttt caaccgcggg cctaccttac ctgattgcgc attcacaacg
     2881 ctttcagaaa agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact
     2941 gaaaattaca tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg
     3001 ttgatgatgg cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat
     3061 ctgtattgaa gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag
     3121 ctcaggcgtt gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag
     3181 ccgcgcgcga tgccgttggc tgagcggatt cttttgttcg tgaaagaccc tggtgtagcg
     3241 tgggtacagc agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt
     3301 agtgcaaaaa tgataataaa tacgcgtctt tgaccccgaa gcctgtcttc ggggtttctt
     3361 tttgcctggt gaatcacaaa aatccccctc ccccgtcacg ctcatatcca gggtaatttc
     3421 gatcactatt tgctatatat tgtgtagttg aatctttttt caactacatc tagtatctct
     3481 gtatcaacag agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa
     3541 atacgaatca tgcgcataac tatttatact cgtaacgatt gcgttcagtg ccacgccacc
     3601 aaacgggcga tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct
     3661 gaagcagcgg aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc
     3721 gatcttagct ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac
     3781 gcggccagtg catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt
     3841 ttatcgaacg tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc
     3901 aggtagacga gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg
     3961 cggtgccacg acaggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg
     4021 gcgttattgc ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga
     4081 ttgcccggaa atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg
     4141 atatcgaaaa cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct
     4201 gacgcaggaa acgatggatt accacgcgct gaatgcgatg cttaatcttt acgatagcgc
     4261 aggtcgcatt cagttcgata aagaccgcca ggccgttgac gcctttattg cgacgcatgt
     4321 gcgtccgaac agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga
     4381 aggttactat gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt
     4441 tgcccacgca cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta
     4501 caccagctat acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg
     4561 agtcacgatg gtggcgctga cgctggcaca aggcgatgag acgctggcgt tgcaactgac
     4621 cgatgaaatg ctgtcaggac gctttcagcc agccacgcca acattcctca actgcggtaa
     4681 gcagcagcgc ggcgaactgg tttcctgttt tttgctgcgt attgaagaca atatggagtc
     4741 gattggtcgg gcggtaaatt ccgcactgca gctgtcgaaa cgcggcggcg gcgtagcatt
     4801 tttgctgtcg aatctgcggg aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc
     4861 tggcgtaatt ccggtgatga aaatgttgga agacgcattt tcctatgcca accaactcgg
     4921 cgcgcgtcag ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt
     4981 tctcgacacg aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg
     5041 cgtggtgatc ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt
     5101 ttcgccctat gacgttgagc gagtttatgg caagccgttt gccgatatcg ccattagcga
     5161 acactatgac gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga
     5221 tttcttccag cgactggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga
     5281 agacacagta aaccgtgcta accctatcgc cgggcgcata aatatgagta atctctgctc
     5341 agaaattttg caggttaaca gcgcctcaga gtatgacgag aatctcgact atgcccgcac
     5401 aggccatgat atttcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc
     5461 cgatttttcc cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag
     5521 tcatatccgc agcgtgccgt ccatcgaagc cggaaatgcc gcctcgcacg ccatcggact
     5581 ggggcagatg aatttacatg gttatctggc gagggaaggc atcgcttatg gttcgccgga
     5641 agccctggat ttcaccaatc tctatttcta taccatcacc tggcacgcac tgcgtacctc
     5701 gatgttgctg gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgctatgc
     5761 cagtggtgaa tatttcagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt
     5821 tggcgaactg tttgcccgca gctgtattac gttacctacc cgtgagatgt gggcgcaact
     5881 gcgcgacgac gtgatgcgct acggcatata caaccagaat ctccaggcgg tgccgccaac
     5941 cgggtctatc tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt
     6001 agagatacgc aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa
     6061 cgaaaatctg gcgctgtatc aggacgctta cgaaattggc gcagaaaaga tcatcgacac
     6121 ctacgcggaa gcgactcgcc atgtcgatca ggggctgtcg ctgacgctgt ttttccccga
     6181 taccgccacc actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa
     6241 aacgctctat tacatccgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg
     6301 cgtctcctgt gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg
     6361 gaacaagata tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg
     6421 gctaccagaa aaggtgccgc tgtcgaacga tattcccgcc tggcagacat taactgtcgt
     6481 agaacaacaa ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa
     6541 tgttatcggc gcgccttcgc tgatgcccga tgcgctcacg cctcatgaag aggcggtatt
     6601 atcgaatatc agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac
     6661 gctgtgccag accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt
     6721 gcagcgaaaa gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa
     6781 aatcgccagt gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta
     6841 tttttccagc cgcggaaagc tgaccaatac cgcggacctg atccgtctga ttatccgcga
     6901 tgaagcagtc cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc
     6961 tctgggacaa cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga
     7021 caacgagttg caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa
     7081 agcgtttctc tgttacaaca ccaataaggc tttgatgaat ctgggctacg aaccgttatt
     7141 tcccgcagaa atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga
     7201 tgaaaatcac gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga
     7261 aacagaagat gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgca
     7321 gcagggaaat aattcccgcc aaatagcttt ttatcacgca aatattctgt agtgatctac
     7381 actgatactc tgttgcatta ttcgcctgaa actacaatat tcaggcgttt tttcgctatc
     7441 tatgacaaaa aatatcaact tttctcgatt tgctctcagc ccttatatca cggaaaattc
     7501 cggcgatttg ctcgcatcaa tattcatgcc acatttgccc tcaggggttg cctcagattc
     7561 tcagtatgtt agggtagaaa aaagtgacta ttcccattgg gtaatatatc gacataaaca
     7621 aataaagaaa tcattctatt gcatggccat taaattaaaa attaaaaatc tttataaaat
     7681 atttggtgag catccacagc gagcgttcaa atatatcgaa caaggacttt caaaagaaca
     7741 aattctggaa aaaactgggc tatcgcttgg cgtaaaagac gccagtctgg ccattgaaga
     7801 aggcgagata tttgtcatca tgggattatc cggctcgggt aaatccacaa tggtacgcct
     7861 tctcaatcgc ctgattgaac ccacccgcgg gcaagtgctg attgatggtg tggatattgc
     7921 caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa aagattgcga tggtcttcca
     7981 gtcctttgcc ttaatgccgc atatgaccgt gctggacaat actgcgttcg gtatggaatt
     8041 ggccggaatt aatgccgaag aacgccggga aaaagccctt gatgcactgc gtcaggtcgg
     8101 gctggaaaat tatgcccaca gctacccgga tgaactctct ggcgggatgc gtcaacgtgt
     8161 gggattagcc cgcgcgttag cgattaatcc ggatatatta ttaatggacg aagccttctc
     8221 ggcgctcgat ccattaattc gcaccgagat gcaggatgag ctggtaaaat tacaggcgaa
     8281 acatcagcgc accattgtct ttatttccca cgatcttgat gaagccatgc gtattggcga
     8341 ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc ggcacaccgg atgaaattct
     8401 caataatccg gcgaatgatt atgtccgtac cttcttccgt ggcgttgata ttagtcaggt
     8461 gttcagtgcg aaagatattg cccgccggac accgaatggc ttaattcgta aaactcctgg
     8521 cttcggccca cgttcggcac tgaaattatt gcaggatgaa gatcgcgaat atggctacgt
     8581 tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc gattcgctta aaaccgcgtt
     8641 aacgcagcag caaggtcttg atgcggcgct gattgatgcg ccgttagcag tcgatgcaca
     8701 aacgcctctt agcgagttgc tctctcatgt cggacaggca ccctgtgcgg tgcccgtggt
     8761 cgacgaggac caacagtatg tcggcatcat ttcgaaagga atgctgctgc gcgctttaga
     8821 tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg ggataccacg ccagcggcgg
     8881 acagtgccgc gcaatccgca gacgcctggg gtacaccgac gactgcaccg gctgacggcg
     8941 gtggtgctga ctggctgacc agtacgcctg cgccaaacgt cgagcatttt aatattctcg
     9001 atccgttcca taaaacgctg atcccgctcg acagttgggt cactgaaggg atcgactggg
     9061 ttgttaccca tttccgtccc gtcttccagg gcgtgcgcgt tccggttgat tatatcctca
     9121 acggtttcca gcaattgctg ctgggtatgc cagcgccagt ggcgattatc gttttcgctc
     9181 tcatcacctg gcagatttcc ggggtcggaa tgggcgtggc gacgctggtc tcgctgattg
     9241 ccatcggcgc aatcggtgcc tggtcgcagg caatggtgac tctggcgctg gtgttaactg
     9301 ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat atggctggcg agaagtccgc
     9361 gagcggcgaa aattattcgt ccactgcttg atgccatgca gaccacgcca gcgtttgttt
     9421 atctggtgcc aatcgtcatg ctatttggta tcggtaacgt gccgggcgtg gtggtgacga
     9481 tcatctttgc gctgccgccg attatccgtc tgaccattct ggggattaac caggttccgg
     9541 cggatctgat tgaagcctcg cgctcattcg gtgccagccc gcgccagatg ctgttcaaag
     9601 ttcagttacc gctggcgatg ccgaccatta tggcgggcgt taaccagacg ctgatgctgg
     9661 ccctttctat ggtggtcatt gcctcgatga ttgccgtcgg cgggctgggt cagatggtac
     9721 ttcgcggtat cggtcgtctg gatatggggc ttgccaccgt tggcggcgtc gggattgtga
     9781 tcctcgccat tatcctcgat cgcctgacgc aggccgttgg gcgcgactca cgcagtcgcg
     9841 gcaaccgtcg ctggtacacc actggccctg ttggtctgct gacccgccca ttcattaagt
     9901 aactctgcac ttgcccggtg acgccgggca ttatcaccct gccaaaaaaa ggaataacaa
     9961 tgcgacatag cgtacttttt gcgacagcgt ttgccacgct tatctctaca caaacttttg
    10021 ctgccgatct gccgggcaaa ggcattactg ttaatccagt tcagagcacc atcactgaag
    10081 aaaccttcca gacgctgctg gtcagtcgtg cgctggagaa attaggttat accgtcaaca
    10141 aacccagcga agtggattac aacgttggct acacctcgct tgcttccggc gatgcaacct
    10201 tcaccgccgt gaactggacg ccactccatg acaacatgta cgaagctgcc ggtggcgata
    10261 agaaatttta tcgtgaaggg gtatttgtta acggcgcagc acagggttac ctgatcgata
    10321 agaaaaccgc cgaccaatac aaaatcacca acatcgcaca actgaaagat ccgaagatcg
    10381 ccaaactgtt cgataccaac ggcgacggta aagcggattt aaccggttgt aaccctggct
    10441 ggggctgcga aggtgcgatc aaccaccagc ttgccgcgta tgaactgacc aacaccgtga
    10501 cgcataatca ggggaactac gcagcgatga tggccgacac catcagtcgc tacaaagagg
    10561 gcaaaccggt gttttattac acctggacgc cgtactgggt gagtaatgag ctgaagccag
    10621 ggaaagatgt ggtctggttg caggtgccgt tctccgcact gccgggcgat aaaaatgccg
    10681 ataccaaact gccgaatggc gcgaattacg gcttcccggt cagcaccatg catatcgttg
    10741 ccaacaaagc ctggaccgag aaaaatccgg cggcagccaa actgtttgcc attatgcagt
    10801 tgccagtggc cgatattaac gcccagaacg ccattatgca tgacggcaaa gcctcagaag
    10861 gcgatattca gggccacgtt gatggctgga tcaaagccca ccagcagcag ttcgatggct
    10921 gggtgaatga ggcgctggca gcgcagaagt aatttttatt cgggcggata aggcgttttc
    10981 gcctcatccg ccgctctgta cacaatgcct gatgcgacgc tgacgcgtcc tatcatgcct
    11041 acaaaccaca tcgcacattt caacaagcta tctttcatcc catattcatc aacatccgct
    11101 attattgatt tccagcttaa tcatcacctg atgaacaaaa ataatgacta aacctaatca
    11161 tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc accggtctgg cggtagccag
    11221 taactattac gcccagccat tgctcgacac catcgcgcgt aacttttccc tttccgccag
    11281 ttcggcaggc tttattgtta ccgccgcgca gttgggctat gccgcaggtc tactgtttct
    11341 tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga ccttactggc
    11401 agcgggcggt atgttaatca ctgccagcag tcagtcactg gcgatgatga tcctcggtac
    11461 ggcattaacc ggtttattct cagtcgtggc acaaattctg gttccgctgg cagcgacgct
    11521 ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt atgagcggtc tgctgttggg
    11581 gatattgctg gcacgaaccg ttgccgggtt actggcaaac ctcggcggct ggcgcaccgt
    11641 cttttgggtt gcttcggtgt taatggcact gatggcgctg gcattatggc gtggtctgcc
    11701 acaaatgaaa tcagaaaccc acctcaacta cccacagttg ttgggttccg ttttcagcat
    11761 gttcatcagc gataagatcc tgcgcacccg cgcgttgctg ggctgcatga cctttgccaa
    11821 cttcagcatt ctctggacct caatggcctt tttgcttgcc gctccacctt ttaactacag
    11881 cgatggtgac attggtctgt ttgggcttgc gggagctgcc ggagcgttgg gcgctcgtcc
    11941 ggcgggcggt tttgccgaca agggcaaatc gcaccacaac tttcggtctg cttctgctgt
    12001 tactttcatg gctggcaatc tggtttggtc acacttccgt actggcgttg attatcggca
    12061 ttctggtgct ggacctcacc gtacagggcg tgcatatcac taaccagacg gtaatttatc
    12121 gaatacatcc tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc tactttattg
    12181 gcggtgccgc cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg
    12241 tttttctggc tggcgcgacg attgccctgg ttaacttact ggtctggtgg cgaggttttc
    12301 atcgtcagga agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa
    12361 gggtgttaag aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat
    12421 attaacaatc gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt
    12481 cggcgacctt catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg
    12541 tggcctttgc gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt
    12601 ttttctcctg catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag
    12661 ccgggagtag tttgtgggtt gctgcactga gcgtcatggc aatggatgtt cgccatgttt
    12721 tgtatggccc gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc
    12781 tatgggcgtt tggcctgacg gatgaggttt ttgccgccgc caccgcaaag ctggtacgca
    12841 ataatcgccg ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt
    12901 gggtatttgg tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg
    12961 ccgttgaagc tgcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct
    13021 ctttccagcg caaacaatct ctttgcgtta ccgcagcttt agttggtgcc cttgcaggcg
    13081 taacgctatt ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg
    13141 cgttaatcca ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg
    13201 ttactggttg gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt
    13261 aatgcccgcc caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc
    13321 tcgatatgcg ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt
    13381 ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc
    13441 attatcatcc caacactgct tagtgcgctg gcctatgggc tcgcctggaa agtgatggcg
    13501 attatataac ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt
    13561 cgttactata tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc
    13621 attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc
    13681 cttctgactc gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg
    13741 ctgaaggctc aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag
    13801 gaaaaccaca gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac
    13861 gcgacgcgta ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg taaaagcgat
    13921 aacgatcgcc gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag
    13981 gttttaccac cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa
    14041 aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa
    14101 gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta
    14161 taaaagaaaa aatgaccggc cagcatcgca acatgctggc ctttttggca agcaggtcgg
    14221 ctcagccgat gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa
    14281 accccgcagc aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc
    14341 ttgctcttta taattattgc cgtagcgata gggatttatt ggtttttggt actgcgtcat
    14401 ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga ttcaaattat gtctcaggtg
    14461 tctggcagcg tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgatgtt
    14521 ctggtcactc tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg
    14581 gcttccagcg ttcgccaaac ccaccagctg atgattaaca gcaaacagtt gcaggcgaat
    14641 attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg actacaaccg ccgtgtgccg
    14701 ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc
    14761 agtgcccagg cgcaactgga cgtcgctatt caacaataca atgccaatca ggcgatgatt
    14821 ctggggacta aactggaaga tcagcctgcc gtgcaacagg ctgccaccga agtacgtaac
    14881 gcctagctgg cgctggagcg tacccgtatt gtcagtccga tgaccggtta tgtttcccgt
    14941 cgtgccgtgc aaccgggtgc gcaaattagc ccaacgacgc cgctgatggc ggtcgttcca
    15001 gccaccaata tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgcatc
    15061 ggtcagccgg tcaccatcac caccgatatt tacggcgatg atgtgaaata caccggtaaa
    15121 gtggttggtc tggatatggg cacaggtagc gcgttctcac tgcttccggc gcaaaatgcg
    15181 accggtaact ggatcaaagt cgttcagcgt ctacctgtgc gtatcgaact ggaccagaaa
    15241 cagctggagc aatacccgct gcgcattggt ttgtccacgc tggtgagcgt caataccact
    15301 aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc ggtaagtacc
    15361 gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac
    15421 gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact
    15481 ggtcattatg acgattgcgc tgtcactggc gacattcatg caggtgctga actccaccat
    15541 tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccaaggaac
    15601 gtgggtaatc acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct
    15661 ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc
    15721 gtcttgggcg tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca
    15781 ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc
    15841 gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat
    15901 ttgcggcccg atcctcggcg gttatatcag cgataattac cactggggct ggatattctt
    15961 catcaacgtg ccgattggcg tggcggtggt gttgatgaca ctgcaaaccc tgcgcggacg
    16021 tgaaacccgc accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg
    16081 tatcggcagc ctgcagatta tgctcgaccg cggtaaagag ctggactgtt tttcatcaca
    16141 ggaaattatc attcttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga
    16201 gctgaccgac gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat
    16261 cggctgcttg tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc
    16321 gcagttgttg caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc
    16381 ggtagggatt attccggtga ttctgtcgcc gattatcggc cgcttcgcgc ataaactgga
    16441 tatgcggcgg ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc
    16501 ctataccttt gaaccaggta tggatttttg cgcgtcggcc tggccgcagt ttatccaggg
    16561 gtttgcggtg gtctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc
    16621 ggaacgactg gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat
    16681 cggcacgtcg ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt
    16741 gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg
    16801 gcttgggatg acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg
    16861 gctgattatt tccgccaatg agatcttctg gatgtcagcc gggatattcc tcgtactgct
    16921 ggggctggtg tggtttgcta aaccgccatt tggcgcaggt ggcggcggag gcggtgcgca
    16981 ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt
    17041 ttactgacta gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc
    17101 tgttgatgcg tacctcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg
    17161 agtgcatctg gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct
    17221 gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg
    17281 gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa
    17341 tctctacacc attaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg
    17401 tatggatccc tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca
    17461 cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt
    17521 ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa
    17581 tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc
    17641 gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactcc cgagtggcct
    17701 ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg tatcagcggt
    17761 ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat cctcttcgcg
    17821 cagaatttcc agcggctctt cacgcagcag attacggtag gcttctgcaa acgctttgcc
    17881 tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc
    17941 gggattatcg aagcaggcaa ccagttcatc acacactttc tgatacgctt cgccgccgtt
    18001 aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg
    18061 cagtaacggg aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg gtttgcgacc
    18121 ttcaaggatc acccggttcc agttaacgcg cgtacaggca agttcgctac tgctcatttc
    18181 cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc
    18241 atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat
    18301 gccgccacgt aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc gttttggacg
    18361 aatcggcgca tacagttcgt tttcaatctg cagcacgttg ctattgattt gcagcctctt
    18421 accgtctttc tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt
    18481 aaggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt
    18541 attggtatag ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc
    18601 gcactcggtt ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat
    18661 cgccggagat gcaccaaaca gataaggaat aacccaaccg aaacgatagt aattgcggat
    18721 aacgcggaaa tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca
    18781 cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg
    18841 catcagcgcg ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt
    18901 gttagaagtg ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat
    18961 acttaacggc cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg
    19021 cataaaggtc agcatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc
    19081 ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg
    19141 acctgttgtt gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg
    19201 ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc
    19261 cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg
    19321 accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc
    19381 aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa aagattactg gcccccagga
    19441 gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa
    19501 taatagcgtg actgcaccat agcgtttcag ccagccggta gctttctctt gccagcgcga
    19561 tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt
    19621 acccattgtt gctgttaaaa ctaaaaccca gggatgactg atcccggaaa gcaacattgc
    19681 caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa
    19741 caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacagc gtccatgcct
    19801 gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt
    19861 tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg
    19921 acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg cgccagcaat
    19981 gcctcagcga tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa
    20041 cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat acttcttact
    20101 gcttctgttt tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata
    20161 attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcatcgc ctgaatatcg
    20221 tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggcgccgta
    20281 tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc
    20341 tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc
    20401 gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag atatttttga
    20461 aggaaatttt ttggagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt
    20521 cgtagccgag tactctatcc agctgagcta cggat
//
LOCUS       DAJVPT010000071        20304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVPT010000071 DAJVPT010000000
VERSION     DAJVPT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..4132)
                     /gene="mukB"
                     /locus_tag="OQM47_002570"
     CDS             complement(<1..4132)
                     /gene="mukB"
                     /locus_tag="OQM47_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1480677.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSI"
     gene            complement(4132..4836)
                     /gene="mukE"
                     /locus_tag="OQM47_002571"
     CDS             complement(4132..4836)
                     /gene="mukE"
                     /locus_tag="OQM47_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1480678.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4817..6139)
                     /gene="mukF"
                     /locus_tag="OQM47_002572"
     CDS             complement(4817..6139)
                     /gene="mukF"
                     /locus_tag="OQM47_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1480679.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6136..6921)
                     /gene="cmoM"
                     /locus_tag="OQM47_002573"
     CDS             complement(6136..6921)
                     /gene="cmoM"
                     /locus_tag="OQM47_002573"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1480680.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7057..7836
                     /gene="elyC"
                     /locus_tag="OQM47_002574"
     CDS             7057..7836
                     /gene="elyC"
                     /locus_tag="OQM47_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1480681.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7813..8706)
                     /locus_tag="OQM47_002575"
     CDS             complement(7813..8706)
                     /locus_tag="OQM47_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1480682.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8860..9606)
                     /gene="kdsB"
                     /locus_tag="OQM47_002576"
     CDS             complement(8860..9606)
                     /gene="kdsB"
                     /locus_tag="OQM47_002576"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1480683.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9603..9785)
                     /gene="ycaR"
                     /locus_tag="OQM47_002577"
     CDS             complement(9603..9785)
                     /gene="ycaR"
                     /locus_tag="OQM47_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1480684.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9837..11069)
                     /locus_tag="OQM47_002578"
     CDS             complement(9837..11069)
                     /locus_tag="OQM47_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1480685.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11106..12092)
                     /gene="lpxK"
                     /locus_tag="OQM47_002579"
     CDS             complement(11106..12092)
                     /gene="lpxK"
                     /locus_tag="OQM47_002579"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1480686.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12089..13837)
                     /gene="msbA"
                     /locus_tag="OQM47_002580"
     CDS             complement(12089..13837)
                     /gene="msbA"
                     /locus_tag="OQM47_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1480687.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13874..16133)
                     /locus_tag="OQM47_002581"
                     /pseudo
     CDS             complement(13874..16133)
                     /locus_tag="OQM47_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16340..16624)
                     /gene="ihfB"
                     /locus_tag="OQM47_002582"
     CDS             complement(16340..16624)
                     /gene="ihfB"
                     /locus_tag="OQM47_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1480688.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16784..18457)
                     /gene="rpsA"
                     /locus_tag="OQM47_002583"
     CDS             complement(16784..18457)
                     /gene="rpsA"
                     /locus_tag="OQM47_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1480689.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18568..19251)
                     /gene="cmk"
                     /locus_tag="OQM47_002584"
     CDS             complement(18568..19251)
                     /gene="cmk"
                     /locus_tag="OQM47_002584"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1480690.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19424..20188)
                     /gene="ycaL"
                     /locus_tag="OQM47_002585"
     CDS             complement(19424..20188)
                     /gene="ycaL"
                     /locus_tag="OQM47_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1480691.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4887 a         5627 c         4923 g         4867 t
ORIGIN      
        1 gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac tctgcgcgca
       61 accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg cggtaatcca
      121 gcagttcttc gccaatggtc tgcggcgtgc gctgccccat atcaatctgc ggattaagac
      181 gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta aacagatcct
      241 gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt tcacgcacgt
      301 tcacgttgag acgcacgctg ttcacctgac caaacgatac gttctgcaac ccctggttga
      361 gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc gccacgctgc
      421 gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga cggctaagtt
      481 caatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata tcctgacgaa
      541 tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc tccggacgtt
      601 tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc accgccagac
      661 gcagcgcacc taacgcctta tccgacatgg aacgcaaatc atcagcggag agataagcca
      721 gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc atcaccgcac
      781 accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc cgctccagct
      841 tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc gctttttcca
      901 gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca cggcgaatac
      961 gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat tcacgttgca
     1021 gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc agcacctggt
     1081 tgtactgact caactgcgct gcgtgaccgc gcaacgcttc gcgagcgcgg gtacgctccg
     1141 cttccgcctg ttccagacgt tcacgcagtt tttcgttgag atcgctgtta ccgctaagca
     1201 tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc gtcagggcaa
     1261 acgcttgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct tcttttaact
     1321 gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt ttcgccagtt
     1381 gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga cgttcgcgga
     1441 tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc ggcagaatgc
     1501 ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc tgctggttat
     1561 cattttcatg attactcaac gcccgctcca gttcgactcg acggctgttc agttgacgga
     1621 tttctgcttc cgggtcagac tcaaacgcca ccgccagatg actgccgata aagcggctga
     1681 acgcctgatg cagacgctgg gttttctgta catcaaagga gagcgtggcg aagcgttcgg
     1741 aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca gcacgaccaa
     1801 acagcggcac ttccgggaaa cgtgaataac gccactgtcg atcggcgatt ttcaccacca
     1861 ccgctttttc cagctcatca acgctgaaca cgctatcatc gaatgactgc ggatcccctt
     1921 cgatcaaata gagatcttcc gggcaatcgg tcaagccttc caggtgttcg gttacctgtg
     1981 acagatctgg caccacgatg gcgtggcgtg acgggccata cagagccgag aagtacggcg
     2041 catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca aaacgctccg
     2101 ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt tcgatctctt
     2161 catcgacggc gtttttgcgc gcgcctactt catcgcgttc aacaatcgcc tcgcgctcac
     2221 gctccagcaa ctgttgcaga tattcagtga cgtcctggct ggaggagaac tcttcgccgc
     2281 actgttcact caactggttg agactgtttt gcgctgccag ccaaaccggt gcacgctgca
     2341 tcaaactctg aatgcgagac tgcagctgtt cctgttcctg ccgcagtgcc atccgctctt
     2401 cacgggcgtt agatacgcta tcggaaagag aggcaatgcg tgcttccagc tcctgatgca
     2461 gagcttccag ttcatcaata tcaaaattct tgccctgacg tttgcagaaa tctgccagca
     2521 gacgctcagc ttcttgctgc tcacgcagac gctgttccag ttcgcttaag cgcatccgca
     2581 acggctgaac ctgctctgcc aggtgacgct gatcaacccc ttcgcgcaat agttcgcgag
     2641 cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc agctgataag
     2701 cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca agagagagca
     2761 ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg gcggcgctgt
     2821 cggcggttaa gtccggcaga tggcacagtt ctttggcacg attaagcgca gcaatcgcct
     2881 ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag tcagcaagct
     2941 ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctca ttctcttcct
     3001 ggcgttcgat ggcttctgcc accacttcat tttgctcttc cagacggatc tgcagctcat
     3061 cgagatccac ttcgtagcgt tcgatcttct cctgctgacg caatgcggtt tgcaccaggt
     3121 tcaggtgatc gctggccgcc tgataatccg cttccagatc accttcggca ccgttgtgct
     3181 ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt tgctgacgcg
     3241 aagtatgtag ctcgcgacga aactccaggg ctttgtcgag atggacacgg cgctcgttgg
     3301 cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga tgcttaaaca
     3361 ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt tcacgcagcg
     3421 ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac aggtagtcgc
     3481 gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg atcagacgat
     3541 agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc agatcgaaca
     3601 tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct tccatcgcct
     3661 cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg ttcagggttt
     3721 cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca aatggtttga
     3781 tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc accacgcgct
     3841 ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc agcttaccgt
     3901 gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta cggaaatgca
     3961 gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catcgtggtg gatttacccg
     4021 ccccgttacc gccggaaagc gtcgtgacca gctcgtcaag gtcaaaagtt cgggcaaaaa
     4081 agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc attattcttc
     4141 ctctccgcta tctggctgac tctcttcggt ttcatcgttg agttgcagat gattttcaat
     4201 cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat cgtcgccagc
     4261 acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cggaacttgc tgctgtcgtg
     4321 gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac gtactttctc
     4381 ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca gtttcagcag
     4441 tttcgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg tgaaaatccc
     4501 ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc cgaccatcat
     4561 atccagttcc gacaagacgg aacgcgggat aagagtggtg gaacgtgggc gtaaatagaa
     4621 gaacccttcc ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact cttccagata
     4681 ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa tatggcggcc
     4741 tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct gcgccagctt
     4801 aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg gctccgtaat
     4861 cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct acgccaagac
     4921 gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc ggatactgtg
     4981 acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt ttgtacacgg
     5041 caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac tcttcgtatt
     5101 ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc tcttcgtcac
     5161 gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca tcaaaatagg
     5221 tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata tcgatcgcgg
     5281 tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg gattgctggc
     5341 cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga tcgacgaaat
     5401 gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc tgcaatttgt
     5461 cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt tccggaagtt tccgaaagca
     5521 acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg gcgatatcgt
     5581 ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata ctgtcgaaaa
     5641 tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga aattcatcac
     5701 cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc gacaactgca
     5761 tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta atgccgatgc
     5821 cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta aagcggttca
     5881 gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg atggtttcgc
     5941 tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg ccttcactca
     6001 tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa gagagtcggt
     6061 ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt tcggggactg
     6121 tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg tgacatgaat
     6181 ataacgccct agggtaatat acggttcctg acggcaataa cgcgtttcta attcaagtaa
     6241 tgcttcatag cagtcgcgct gctggtactt ctcgcgcaga taatcatgaa acacgcgaac
     6301 gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa cctgcgccgg
     6361 gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc ccgcctgcac
     6421 gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat tgtagaacat
     6481 taacgacaac actccgcctg gacgcaacac tgaccacagg gtctgcaata cactgcgggg
     6541 atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg tttccaaatg
     6601 cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca caccttttgc
     6661 ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata aaataacctg
     6721 atgcccgcgc tcggccattt tgattgcggt ctgcccttct ccgccgccag catccagcac
     6781 acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc acagaatagc
     6841 ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt ccgcaatatc
     6901 atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt ttctgataaa
     6961 gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata tcgctagcga
     7021 tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact gaaaaaagtg
     7081 attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc tggcctggcg
     7141 ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat tgggtggctg
     7201 gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc catcgaaagc
     7261 acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct cggaggtggt
     7321 tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag tctgccacgc
     7381 ctgaatgagg gtatccgttt atggcgcgaa aatccgggtt caaaactgat cttcacggga
     7441 ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt tgcgcaatcg
     7501 ctgggcgtac cgcgcgagca aattatcacc ctggatttgc caaaagatac cgaagaagaa
     7561 gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac ctccgcatca
     7621 catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct accagccccg
     7681 gcgaatcagt tggccatcga ctcgccgctt aatccgtggg aacgggcaat cccttctccg
     7741 gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat ctggcagtgg
     7801 ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat cgaagttgcg
     7861 ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca catacaacca
     7921 acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc agagcaaatc
     7981 ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg cccaaagcat
     8041 tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca gcatgctgcg
     8101 taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa ataatgccgg
     8161 gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac cggtgttatt
     8221 gaactgattg agcgtggtcc atagcacttc aacatgttgc cggtaccatg agggccagaa
     8281 attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac ggtgccaggc
     8341 cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg ttcgggctgg
     8401 tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc cggggtgttc
     8461 ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc ttgccagcat
     8521 ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc ttgccattaa
     8581 cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg ctttttcatt
     8641 gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg cacgcagctg
     8701 ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca atataaaaga
     8761 tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt aacggtacga
     8821 cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt cagcgcgaac
     8881 gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag caacagcaac
     8941 atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga tgtgttctaa
     9001 cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc cataaatacc
     9061 aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac gatcacgatc
     9121 ccaaggaatg gtggcacgtg agaagtacag tgcataccct tcagcgtcga gaaccacttt
     9181 caccgcattc gggttaaacg cttcttccgc attgtggatt ggcaccgcca gagtcgccat
     9241 acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg cagggatcat
     9301 cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc atttttcgac
     9361 aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac atacttcacc
     9421 gccagcggct tcaacggcgc gggcaacatc atcatgatcg gttgccacga tgatgcgctc
     9481 ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc cgttaatatc
     9541 aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa tgaccacaaa
     9601 actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag caacactgga
     9661 atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc ttgtttttcc
     9721 tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag caaacgatga
     9781 tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat aatcatttat
     9841 gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg cgggcagcgt
     9901 cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc ctgacgtaac
     9961 cctttttgca gcattgtcgt tggtttaatg ccttcctgta accacagaga gataacttca
    10021 aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg cccacgatgt
    10081 aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata gcactccagc
    10141 cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat cccagacaac aggatcaaaa
    10201 ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg ccagcgcacg ctcgagtagc
    10261 ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac agcaatgatt
    10321 tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt cagccgatag
    10381 taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt atccagcatg
    10441 atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc aggcatgaca
    10501 cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat cacctttccg
    10561 gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca gccgcttgca
    10621 cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ctttatcatg aatatgctga
    10681 attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta tttccagccc
    10741 attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg cggcataaag
    10801 caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc atccagccac
    10861 tgggcaggat aatttcccag acgactgaaa agcaccagat atggactacg ggcaacaata
    10921 ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat atcctccaac
    10981 gacgctcgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag attacgcgca
    11041 tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac gctgccgcgg
    11101 cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc tggttcatca
    11161 cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa ggcccggcat
    11221 ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa cgcactgaca
    11281 tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg ttgtacgcca
    11341 cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat cgccactaca
    11401 tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc ctgacccggc
    11461 agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat taccgcatca
    11521 accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca ccagccattg
    11581 ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag acggtaatgc
    11641 tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag aatggctttt
    11701 accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtgcgttg ataaatcaac
    11761 acaggttcat cacccgcctg tgctgtggtg gtatctgccg acaataacag cggataagat
    11821 tcagccttac caccatatcc ccgcgatacg acccccacgc gaataccgcg ctgttgcaac
    11881 tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcagt gagattacca
    11941 accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt atagcaaaga
    12001 cggatcgcgc cactcaccag gccatacaac caggagagtg gcagcaatag ccgccacaaa
    12061 ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg tgaagttgcg
    12121 cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc gcgttccaca atgacaccat
    12181 cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg gcaatcacca
    12241 gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt tcggattcgg
    12301 tatccagagc cgaggtagct tcatccagaa tcagaatcgg gctatcacgc agcaaggctc
    12361 gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt tcaccaatca
    12421 ctgtatcgag accgttatcc atcttattga tgaagtccat ggcgtaggcc atccgcgccg
    12481 cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc gtaagcaatg ttgttagcaa
    12541 ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta cgtaacgacg
    12601 ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca tcaatatcgt
    12661 aaaaacgcgt gatcaggctg gcgatggttg atttacctga accagagcgt ccaaccagag
    12721 caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcgggt acgtcacgtc
    12781 ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc agtcgcacgc tcgatcacgc
    12841 gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtc tgacaagccg
    12901 ccataccgcg ctggaactgg gcgttaacgt tggtcagcga tttcagcgga cgcatcagtg
    12961 caatcattga agagaaaaca acggtaatcg taccggcagt caggttatcc ataacacttg
    13021 ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc tgaatgatcg
    13081 gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt cggttactga
    13141 ctttatcgaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact tctttgtggc
    13201 ccttcagcat ttgttctgca ctggtggtca cttgccccat ggtgttctgc atgtttttac
    13261 tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat cgaaacaatc ggtgccagca
    13321 caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac aggccgatga
    13381 tcgacgcacc ttcacgcaca acagtaatca gtgcgccgga agaagaagaa gcaacctgtt
    13441 cggaatcgta ggtgatacgt gacaacagcg tacccgttga ctgtttgtca aagaatgaaa
    13501 ctggcattcc catcatgtga ccaaacaggc ggcgacgcat ggtcattacc acctttcctg
    13561 atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa atcatcagtc
    13621 cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca aagccatcat
    13681 caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg aggattaacg
    13741 ctacgcccgc cacgatcaga cccgctttga aaggcgcaat ggttggccac agtcggcgga
    13801 atgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc atttgttgaa
    13861 atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga taccaacgag
    13921 gtaaaatttg ctctctgagg ctgctaatcc gccagccttg tgctgaaaaa ttgaccgtca
    13981 cttgcccctg atgtggagta tcaagccatt gatatccttt ctgttgatag cgatgcttaa
    14041 ctttgttaga gggcaatcgc catgcgttat agcgcgatgc cgatgcgagt gccacttttc
    14101 cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg tgaggtacct
    14161 gaagcaatgt tgcctgcact tgctgccagt aacggcttag catcttttgt tcagctgggg
    14221 cttcaatatc accggttaga agaatgctat tcgtcccgtc atcaacctta accacacagg
    14281 aatggttatt tcccttatcg ttgctacctt gtaaaggcca gtgcgcgctg aaacgcaatc
    14341 cttgccactg ccacgcttcg ccacgcacac agggctgatg atgttcccag tttaacggac
    14401 ttctgatcca taacatcggc catatatgca atattgaatc cagccctccc cggtgatcca
    14461 gatgttcatg gctcagaata acgccttccg gttcaagatt atgccagtgg agccagggga
    14521 taatcagttg ttgcccacta tccccttcag gccagtcctg tgtcatagag aatcgctttg
    14581 ccgtttctgg ctatcaccat tgccagccct tgcccgacat caagcatgta cagctgccac
    14641 tcgtcaggtc gaggtttttg ccacagcggc cagcacatca gcaagcctcc agccacacac
    14701 attgctggca acgttcgcca ggcgtttaat cgccatacca ccagtaagaa ccatggggaa
    14761 aatgatagcc attgccaaca ttcaacaatg ttgatccacc cttccggcaa actctttaac
    14821 ccccagaaaa gtaaagccaa agaccggtcg gcaagaaacc ataacccctg ttccaggatt
    14881 aacggcccgc taaaatgcac aaccatcgcg gcgaggatca acggaaccgt gataaatgtc
    14941 accaagggaa ttgcaaatag atttgcaata aacgaggtca gactaatgcc atgaaatatg
    15001 acgatttgca tgggcataag caggagtgtg attcccagtt gcagatggat gagggaaaca
    15061 actgcacgca ataccggcgg cagttgccac tcaggacagg gaaaccactg ataccaaaaa
    15121 atcagtgccg cgaccgcagc ggcagagagc cataaacttt gcgagagaat ggcaacagga
    15181 tccatcagca aaattgccgc cagacaacat atccatacat cccagccact ccactgtcgc
    15241 ccacttaact taagcattcc ccacgtagca agcgccacca cggtacgcaa tgcaggaggt
    15301 tgcattcccg tcaaccaggc ataaaaagca gcacagcaga ttccgccaat taatggtatt
    15361 tgccagtgga tccagcgccc aggcagaaaa atttgtccac tgcgaatgag tccggcagcc
    15421 agcaacgccg caaaagcgat gtgcaatccc gatatcgcca ttaaatgcgc cgttccagta
    15481 tcgcgcatga tatttttgat ttctttgggg acggataacc gttcccccat acctaaacca
    15541 agaataaccg cattccacgg atagggttgc agcgttgttt gtagtgacgc cagatactgt
    15601 gcacgcaggc tacaattcgg ttcaatgaca cttgcctgca gaaaacggcc ggtgagcggc
    15661 tgatgctggg caatggcata acgctggcta tcaaagccgc catcattaag ttggccatga
    15721 actgcacgaa ctttgagttt cattgaccat tgttgtccag cacaaaccgc ttgcggaagt
    15781 tattcgccat acagcacaag gcctggcgca gggaatatac gtcgaccttg tagataagta
    15841 atttgaccgt aatgagtggt catgccgtca gttgcagtga tctcaacaat tgcatcctgc
    15901 gtcgcgccag ttaaggtttc tcctgcccac aaaatttgct ttgctgataa tatgccccaa
    15961 acgaaaaaca gcaacgtcag cgcgatataa cggacggttt taacagggat aaatgccagt
    16021 acgcaggcga agagagtcag aaacgcaagg gttaatgtcc taggcaattg gggcaaaatc
    16081 agcaacggaa aaattccgca aattatgcat acgccgactg tcgttatttt catgcacccc
    16141 tccctgttta tggaaagtgt gcaactttgt tgagcggttg tctggtggga attttgcgtt
    16201 ccccgaggca cattcagaag aatttaacga aagggtgaaa actgctcaaa actctgcgag
    16261 gcagcttcca gttcctgatt agagaattga aaaaagcacc cgacaggtgc ttttctcgcg
    16321 ttcaagtttg agtaaaaact taaccgtaaa tattggcgcg atcgcgcagt tctttaccag
    16381 gtttaaagtg aggaacgtat tttccttcca gttctacttt atcgccagtc ttcggattac
    16441 gtccggtacg tggtgcgcgg tagtgcaaag agaaactgcc gaaaccgcgg atttcaatac
    16501 gctcgccctg cgcaagagtc gaggccatat gctccagcat ctcttttact gcatcttcaa
    16561 ccgtcttggc gggaatgtgc gattgctggg tggcaagtct ttctatcaat tctgacttgg
    16621 tcatgattcc tccggttcct taaaggcaaa ttaagcggct gctgttgctg cattgattaa
    16681 gggcggccgt agccgccctt agtgcttgat tacaggacga aacctgcaat ctgtcaagta
    16741 aactcaacaa acttcggaat aaaaatcccg aagagtcaga gaattactcg cctttagctg
    16801 ctttgaaagc ttcagccatt gcgttgttgg agaagtttgc atcttcctgt ttgttaacag
    16861 ttgcgattgc atctttctcg tcagcttcgt ctttcgcacg aacagacagg ctgattgcgc
    16921 ggtttttacg atcaacgccg gtgaatttag cttcaacttc gtcgccaacg ctcagaacca
    16981 gggtagcgtc ttcaacgcgg tcacgggatg cttcagaagc acgcaggtaa ccttcaacgc
    17041 cgtcagccag ttctacggtt gcgcctttag cgtcaactgc agttacttta ccggttacga
    17101 tagcgccttt cttgttcaga gcaacccagt tgttgaacgg atcttctgcg agctgtttaa
    17161 cgcccaggga gatacgttca cgttctgcgt caacctgcag aacaactgca gcgatttcgt
    17221 cgcctttttt gtattcacga actgcttctt cgcctgcaac gttccaggag atgtcagaca
    17281 ggtgaaccag gccgtcgatg ccgccgtcca ggccgatgaa gataccgaag tcagtgatag
    17341 acttgatttt accttcaaca cggtcgccct tgttgtgggt ttccgcgaac tgctgccacg
    17401 ggttagcttt gcactgtttc agacccaggg agatacgacg acgttcttcg tcgatatcca
    17461 gaaccataac ttccactaca tcgccaacgt taacaacttt ggacgggtgg atgtttttgt
    17521 tggtccaatc catttcggaa acgtgtacca gaccttcaac gccttcttcg atttcaacga
    17581 agcagccgta gtcagtcagg ttggtcacgc gaccagtcag tttggtacct tccggataac
    17641 gtttagcgat agctacccac ggatcttcgc ccagctgttt caggcccagg gatacacggg
    17701 tacgttcgcg gtcgaacttc agcactttaa cagtgatttc gtcgcccacg ttgacgattt
    17761 cgctcggatg cttaacgcgt ttccaggcca tgtcagtgat gtgcagcagg ccgtcaacgc
    17821 cgcccagatc aacgaatgca ccgtagtcag tgaggttctt aacgatacct ttaacttcca
    17881 tgccttcctg caggttttcc agcagctgat cgcgctctgc gctgttttcg gattcgataa
    17941 cggcacgacg agaaacaaca acgttgttgc gcttctgatc cagcttgatt actttaaatt
    18001 caagctcttt gccttccagg tgcagagtgt cacgcaccgg acgaacgtct accagagaac
    18061 ctggcaggaa cgcacgaata ccgttcagct caacagtgaa gccgccctta actttgccgt
    18121 tgataacacc ggtaacagtt tcagcatctt cgtaagcttt ttccagcgtg atccaggctt
    18181 cgtgacgttt agctttctca cgggacagca gagtttcacc gaagccgtct tctactgcgt
    18241 ccagagcaac gtcaacttcg tcacctacct ggatttccag ctcgccctgg gcgtttttga
    18301 actgctcagc cgggatggcg gactcagatt tcagaccagc gtcaaccagt actacgtctt
    18361 tgtcgatagc aacaacaacg ccacgaacga tagaacccgg gcgggtttcg atttctttta
    18421 aggactcttc aaagagttga gcaaaagatt cagtcatgtt taatcttcag gtttatattt
    18481 aacgtccacc tggctccatg ccggatgggg ttgtttaaca tacccgctgt cattccattg
    18541 caacgggggt actgcaaatt cggtcgctta tgcgagagcc aatttctggc gcgcgtattg
    18601 tagcgctttt tcaatcaatt gctcaatgct taaggtggtg gaatccaaca ctaaagcatc
    18661 ggctgccgga accagtggcg ctaccgctcg gttacgatcg cggtcgtcgc gttctttgat
    18721 ctcggccaaa aggcgctcga agttaacact aaagcccttc tcctgcaact gtagcatgcg
    18781 gcgatgcgca cgttcttccg aggaggcgtc aaggaaaatt ttcactggtg catcagggaa
    18841 taccaccgtt cccatgtcgc ggccatcggc aatcagacct ggtaattcgc gaaacgcgcg
    18901 ttggcgacgc aataatgctt cacgaacgcg tgggaatgcc gcgacttgtg aagccgcatt
    18961 cgccacttcc tgagtacgaa tttcgccgct gacatcttcc ccttcgagga tcacttccag
    19021 attgccattg gtcgacacaa aacgtacatc cagatgggat gccagcggta ccagcgcatc
    19081 ttccgacgca acatcaacat ggtgatgtaa tgccgccaat gccagtacgc gataaattgc
    19141 acccgagtcc agcagatgcc attgcaacgc ttccgccata gccttacaca aggtgccttt
    19201 ccctgcaccg cttgggccat caatggtaat aaccggggca attgccgtca tctttatctc
    19261 cttaaaacag gcgtaccgtt aacataaccg cgcgcattat acgcgccgac gccacaaatc
    19321 gttaacgttg tgtgcaaaaa ccagttttgt ctgtattcag atggggattt attgcgcaat
    19381 tatagcgggc tggcagaaga ccagcccgaa agatgacaat gatttactta ccagaggcga
    19441 tacgatcacg gatgtgttgc gcacgctctg ttgatggtgg gtgagagtca aacatggact
    19501 gggtgcgacc gccatccagg ctagccagtt tttcaaagct gccaaccagc ccctgggtgc
    19561 tgatgccacg tttcttcaac agatcaaaag agaaatcatc tgcttcggac tctttatcac
    19621 gggagtattt agcattaata gcgccttctg cgatatcacc caattgtgag cgggaaagct
    19681 gggaagccac accgctggta gctgaaatgg catcgcgtgc ggcaacgatc gcataagaag
    19741 ctttcatttc agccagcgag tgacccaacg cgacgtggcc cagttcatgg cccagaacac
    19801 cttcaatttc gttgtcattc atcatgtcca tcaggccact gtagacacga acacagccgt
    19861 tcgccatcgc ccatgcgttg acgtcgctgg tcatataaac cttatagttg accggcgtgc
    19921 cattaatatt gttacccaat gctttggcga tttttgccag acgtttaccg tacttgctgg
    19981 atttacttgc gacttgattg ccgctgtcca tttcggcaca gccctgattg gcaatcgctt
    20041 ttgcatcggc atcgcttaat gttgctgctt tataggcatt taaaccggag ctgatagcca
    20101 tattggtatc aataccgtgg gtattttgac accctgtcag taatgctgca gaggtcgcaa
    20161 tcgccagcag taatttagta ttcttcatgt gaatcatccg ttgattaatc atatataaat
    20221 attatgtatc aacaacttac attgacaagc gacgctacca ttaatcaaaa tgactcacaa
    20281 ggtccgaaaa aaaacgccga caat
//
LOCUS       DAJVPT010000072        20183 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVPT010000072 DAJVPT010000000
VERSION     DAJVPT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..157
                     /locus_tag="OQM47_002586"
     CDS             <1..157
                     /locus_tag="OQM47_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1480692.1"
                     /translation="DGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALAKVIP
                     FFNTHLG"
     gene            175..813
                     /locus_tag="OQM47_002587"
     CDS             175..813
                     /locus_tag="OQM47_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1480693.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1104..1832
                     /gene="mpaA"
                     /locus_tag="OQM47_002588"
     CDS             1104..1832
                     /gene="mpaA"
                     /locus_tag="OQM47_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1480694.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1807..2772)
                     /gene="ycjG"
                     /locus_tag="OQM47_002589"
     CDS             complement(1807..2772)
                     /gene="ycjG"
                     /locus_tag="OQM47_002589"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1480695.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2891..3397
                     /gene="tpx"
                     /locus_tag="OQM47_002590"
     CDS             2891..3397
                     /gene="tpx"
                     /locus_tag="OQM47_002590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1480696.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3441..4982)
                     /gene="tyrR"
                     /locus_tag="OQM47_002591"
     CDS             complement(3441..4982)
                     /gene="tyrR"
                     /locus_tag="OQM47_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1480697.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5130..6191)
                     /locus_tag="OQM47_002592"
     CDS             complement(5130..6191)
                     /locus_tag="OQM47_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1480698.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6188..7585)
                     /locus_tag="OQM47_002593"
     CDS             complement(6188..7585)
                     /locus_tag="OQM47_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1480699.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7741..8739
                     /locus_tag="OQM47_002594"
     CDS             7741..8739
                     /locus_tag="OQM47_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1480700.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8850..9722)
                     /gene="ompG"
                     /locus_tag="OQM47_002595"
                     /pseudo
     CDS             complement(8850..9722)
                     /gene="ompG"
                     /locus_tag="OQM47_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9795..10876)
                     /locus_tag="OQM47_002596"
                     /pseudo
     CDS             complement(9795..10876)
                     /locus_tag="OQM47_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10890..11549)
                     /gene="pgmB"
                     /locus_tag="OQM47_002597"
     CDS             complement(10890..11549)
                     /gene="pgmB"
                     /locus_tag="OQM47_002597"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1480701.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11546..13813)
                     /gene="ycjT"
                     /locus_tag="OQM47_002598"
     CDS             complement(11546..13813)
                     /gene="ycjT"
                     /locus_tag="OQM47_002598"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1480702.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(13810..14433)
                     /locus_tag="OQM47_002599"
     CDS             complement(13810..14433)
                     /locus_tag="OQM47_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1480703.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14430..14852)
                     /locus_tag="OQM47_002600"
     CDS             complement(14430..14852)
                     /locus_tag="OQM47_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1480704.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(14874..15649)
                     /locus_tag="OQM47_002601"
                     /pseudo
     CDS             complement(14874..15649)
                     /locus_tag="OQM47_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(15667..16719)
                     /locus_tag="OQM47_002602"
     CDS             complement(15667..16719)
                     /locus_tag="OQM47_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1480705.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(16750..17592)
                     /locus_tag="OQM47_002603"
     CDS             complement(16750..17592)
                     /locus_tag="OQM47_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1480706.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17579..18460)
                     /locus_tag="OQM47_002604"
     CDS             complement(17579..18460)
                     /locus_tag="OQM47_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1480707.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18481..19764)
                     /locus_tag="OQM47_002605"
                     /pseudo
     CDS             complement(18481..19764)
                     /locus_tag="OQM47_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(19778..>20183)
                     /locus_tag="OQM47_002606"
     CDS             complement(19778..>20183)
                     /locus_tag="OQM47_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCS1480708.1"
                     /translation="ILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         4908 a         5537 c         4898 g         4840 t
ORIGIN      
        1 tgatggactg gaagtctatg gccgggcgat ggtttcgcag gatcgtcagt tagtttcgtt
       61 acccgatttc tcacattatg aactatatga caaaccggaa gcagttcagg aggcgctggc
      121 aaaagttatt ccttttttca acacccatct gggataagtt tcatgagaaa tattatgaaa
      181 aatgtactga ttcttggtgc tggcggtcag atagcccgcc atgtgattaa tcaactggca
      241 gataaacaaa ctattaaaca aactttattt gcccgtcagc cagcaaaaat acacaaaccc
      301 tatccaacta atagccagat tattatgggg gatgtactga atcatgcagc actgaagcag
      361 gccatgcagg gacaggatat tgtgtatgcc aaccttacgg gggaagattt agatattcag
      421 gcaaattcag tgatcgctgc gatgaaagcc tgtgatgtta aacgtctgat ctttgttctt
      481 tccctcggta tttatgatga agttccagga aaatttgtcg aatggaataa tgcagttatt
      541 ggtgaacccc taaaaccatt ccgcagggct gcggatgcaa ttgaggcttc tggactggaa
      601 tatacaattc ttcgccctgc ctggctgact gatgaagata ttattgatta tgagcttacc
      661 tctcgcaatg aaccattcaa aggtacgatc gtatcacgta agagtgtcgc agcattaatt
      721 acagatatca tcgataaacc tgagaaacac attggtgaga atataggcat caatcaaccc
      781 gggactgatg gcgataagcc attcttcatg taaatcaatt atatagatac catcgtactg
      841 tactatgtgc agtacgattt attaaactta ttaaggtgaa ctgatcaatc agatgcgatt
      901 aacatgtgta gaaccagtaa gaataaaata cgccatgtat ataacattac gacaattcta
      961 atatctgcac atcaatgaaa atgcaaacaa cttccggcaa aaaagtgata caagatcgat
     1021 gacatagcgt tgaagaaaag gatttgataa tccgcaaaat ttgcacaaca ctgcctgtaa
     1081 tacttcctga accagagacg atcatgaccg taacccgctc acgcgccgaa cgcggcgcat
     1141 ttccgcccgg aacagaacat tacggacgtt cattattggg cgcgccgtta atctggtttc
     1201 ctgcccctgc agccagccgc gaaagtggtt tgattctggc tggcactcac ggtgatgaaa
     1261 actcttcggt cgtcacgctc tcctgtgctc tgcggacttt gacaccttct ttgcgccgcc
     1321 atcatgtggt gctgtgtgtg aatcctgacg gctgccagct ggggttacgg gccaatgcta
     1381 atggtgtgga tttaaacaga aactttccgg cggcgaactg gaaggaaggt gaaacggttt
     1441 atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt actgacaggt gacaaacctg
     1501 gctccgaacc tgaaacccag gcgctgtgcc agcttataca ccgcattcaa cctgcctggg
     1561 tggtctcatt ccacgatcca ctggcttgta ttgaagatcc cagacacaac gaattaggcg
     1621 aatggctggc ccagtcgttt gaattgcctc tggtaaccag cgtcggttat gaaacgcctg
     1681 gctctttcgg cagttggtgt gcagatctca acttgcattg catcaccgca gagtttccgc
     1741 ccatctcctc cgacgaagcc agcgaaaaat atctctttgc gatggcaaac ttgctgcgct
     1801 ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc gccggttcca catctaccgc
     1861 cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc ggcaccagcg gtaaagcggc
     1921 gctaatggca cgagaggtac acaacatgca gcccagcatc agactgaaac cttgtgcacg
     1981 cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg gttttatcga gcttaatgtt
     2041 aaccatctca tagcgccctt tcagcgcctt cagattgcta cgagtatgac aactttcatc
     2101 agcacaaatc ggcaacggat gaataaaatt ctccagcgcc gcatcgtcct gcgcaggaag
     2161 cggttgttca agcatcgcaa cgcctaaatc cgccaatagc tggcaacgcg ccgccaaccc
     2221 ttctgcacgc caggattcat ttgcatcaac gatcagcgtg gcatcgggca cagcggtgcg
     2281 aattgccacc atccgctcac tgataagatg gttatccagc ttcactttca gtaatttcgc
     2341 gcctgcctgc cagagtgttg atgcactatt ggccatctga tcaggcgtac cgatgacaac
     2401 cgtctgtgca gtgataactg tctcgggaag cgttatgccg atcaaatcag ccagcgattg
     2461 ctgctgtttt cgcgccgcga gatcccacaa tgcacaatcc agcgcattac gtgctgcgcc
     2521 ggcagggaga attttttgca actcctcccg tgtcagccct ttctctaatt gcggcacgac
     2581 gctcataatt tgcgccatta ccgaggcatc actttctcca taacgcggat acggcgtgca
     2641 ttcgccggtg cctttaatac cctcttcttc cagttcaacc accaccacgc gcgcttcact
     2701 gcgacttccc cgggcaatca caaacggggt atgtaagggc caggcttcct caaatacctt
     2761 aacgattctc atctttactc cttgttgtcg cggtggtctg cggattaagc gcaaatagcg
     2821 tttgctgtgt tattgacagt tagcataaac taggtgtgac gttaactata tgtaaacagg
     2881 aaagataatc atgtcacaaa ccgttcattt ccagggcaac ccggttacag tcgccaattc
     2941 catcccgcag gcgggtagca aagcgcagac ttttactctc gtggcaaaag atctgtctga
     3001 cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg aacattttcc cgagtattga
     3061 taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa ctggcaactg agatcgacaa
     3121 caccgttgtg ctgtgtatct ctgccgatct gccgttcgcc cagtctcgtt tctgcggcgc
     3181 agaaggtctg aacaacgtta tcaccctctc cactttccgt aacgctgaat ttctgcaagc
     3241 ctacggtgtg gcaattgctg atggcccact gaaaggtctg gcagcgcgtg ccgttgtggt
     3301 tattgacgaa aatgacaatg tgattttcag ccagctggtg gatgaaatca ccaccgagcc
     3361 ggattacgaa gcagctctgg ctgtactgaa agcataagtt aatatgcctg atggtgttgc
     3421 accatcaggc atattcgcgc ttactcttcg ttcttcttct gactcagacc atattcccgc
     3481 aacttattgg caatcgcggt atgtgaaacg ccgagacgtt ttgccagttt gcgcgtgctg
     3541 ggataattgc gataaagctg ggttaatacc gagcgttcaa aacggctggt gatttcgtcc
     3601 agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg cggcgtcata atccggcaac
     3661 aaaatatcct gtggacgcag ctcataaccg tccagttgtg tcagtgcgcg atagatagcg
     3721 ttctttaact gccgcacatt tcccggccac gcataacgtg taagtacagt attcaggtca
     3781 gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa agcgggcgac gaacagctca
     3841 gttaacggca taatgtcctg cggacagtca cgtagcggcg gcagattgag cgtcaacacg
     3901 ttcagacgat aatagagatc ttcacggaac acgccttttt gcaccagttc gaccagattc
     3961 ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct catggtcttc gccaacccga
     4021 cggaaagtgc catcattaag aaaacgcagt aatttcgcct gcatccgtgg tgacatttcc
     4081 cctatttcat ccaacagcac cgaaccaccg ttcgcctgct caaagaaccc tttcttccct
     4141 tccggagcat gaccaaacag ttcactctcg accgcatctt ctggtataga cgcgcaattc
     4201 agcgccaggt aaggtttgcc cgctctgggg cttgcctgat ggcaggcgta ggcaaaaaga
     4261 tctttacctg tacctgtgtc acccgtaatc agcagcggcg cgcttagcat cgccagtttc
     4321 tgcgcctgtt cgacaacgtg cttcattttc gggctgacgg cgacaatttg gctgaaggcg
     4381 ctgacgtcct gggcggcgac attttgcaac tggcggccca tacgaatcgt tgatcgcaac
     4441 atcaccaccg caccggtcag gacgtgttga tcattttcat cctgaagata aacaggcgta
     4501 atctccatca ggaaattctg cccattaata acgacatgct cgttatgcga atcttgcggt
     4561 tcgctttcca gccaacgtaa aaaattaaag ccgttaatca attgtgcggc ggtatggttg
     4621 cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc catatccact
     4681 ttgcttttca tatcgacaga gagcacaggt tcaggcaacg cctccagtaa cgcgctcaac
     4741 gccagatgct cacgttcgga aggcatccac gggacagtac gcacatcggt aacacctgca
     4801 atacggcgta tttcggccat cagactgctg aaactctcaa actccagttc agcaaaattg
     4861 aggtagattc gcccaatggg atcaatctca ataccgcgta aatcaatgcc tcttagcacg
     4921 agtagatcga gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac ttccagacgc
     4981 atgggaacct tcacctgaaa aaagaacaat taatatgatg atatgacact atttggatag
     5041 caggaaggtt tctgtcaaca atcattgaca caaagacgga aaaacgggca cttcggcgta
     5101 aagatatccg gtcagcaccc gatattgcat tatttttcgc tgggcgtttt gcctttttgc
     5161 agagtttctt tcacctgacc gataagctga cgacggaaat ccccaaggcg aggtttgtca
     5221 tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt taatcccgag tcgtaccgtc
     5281 agaagtccag caccaattcc ctgagctgcg cgggtagaca aacgagcagc gagatcttgc
     5341 gacatccagt ccatccccac ttcgcgcacc agttcgctgg ctccggcaaa agcgatattc
     5401 agcaatacca gcttaaacag acgcaaacgg ctgtaatacc ccagttcaat gccatacagc
     5461 gtggcaatgc gattaattaa acgcagattg cgccaggcga taaacgccat atcgaccaac
     5521 gccagcgggc tgaccgcaat catcaacgtt gattccgccg ccgaacggct gatttcgcgc
     5581 cgcgcctggg catctaaaac tggctggacc aaatgcgcat acaggctgac cacttcacga
     5641 tcgttctgcg tttcatggat tgaggcatac cagcgttgca gcgccggatg cgactgatca
     5701 atacccgcct gctgcgccag tttttcgcaa aatgcgcgac ctttgcccgt gccgtggcta
     5761 tgcaataaat cacgcgcttc gtcgcgttca tgggcgcgct gtcgcaagcg ccataagcgc
     5821 cgccactctg ttaccacaga acctacgcca gcgccgataa tcaatgcccc agcggcacat
     5881 ccacccagcg ctacccagtc ctgggtttgc caggcattca ttgtccactg tataccctgc
     5941 ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca ccattttgcg ccacaggctg
     6001 cgtttcggac gtaacgctgc gtccattacc gcttcgactt gcccctcttc ttcctgcgct
     6061 tcgtcgagcg tggccggggc aaaattttgc gcctgatttt cgtcaaaggt ctgctgcgcc
     6121 ctgaattttg gattctgttc gacctccagc ggaccgtcga aatcaatacg tggttttaac
     6181 ggttcggtca tcgcaattta tctcctatta aaaattccag cgcggcatca agacgaatat
     6241 gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa cgcttcaaac tggaaccctt
     6301 gcttgtccca gaacgcctga ccgggcaaac gcgcaggaac ttcaccgggg taaacggtga
     6361 gaagcgcacc atcgctgagc cggttaccgc gcaatgccgg aattttctcg ccgttgacgt
     6421 caataatgcc gctggtggtc gcctgcaccg aggccagccc caggcaatcc atactaatcc
     6481 tttcaaacgc cgcattttgc caggcatcct gaatcagttg ttgcagtaat gaaaccatat
     6541 tggcgtgctg atcgaccgtt acatggtccg ctttcatcgc ggcaaacagc agtttgtcga
     6601 taaccggcga gaataagcgg cgaaacagcg tgcgctgccc gtagtggaaa ctttgcatca
     6661 gctgcgtcag tgccagacgc atatcattaa atgcctgtgg cccactgttg agaggttgca
     6721 ggcaatccac cagcacaatc tggcggtcaa agcgcagaaa atgattctta tagaacccct
     6781 tcaccacttt ctcgcagtaa taattaaacc gctcgcgcag cattccggca ttggtgtgct
     6841 tatcggcctg cgccagtttg gactcgtccc aggcatcgac atccggccac gggaagaatt
     6901 gcagcgcggg cgcacctgcc atatctcccg gcaagacaaa gcgcccaggc tgaataaagt
     6961 gcagcccctg ctgtttacag tggtggagat aatcggtcca cacggcggca atgtctgcca
     7021 gacggttttc gtcggcaggc gctagcgggt caagtccttc gcacatcatt cgccatttcg
     7081 ccgaccattc gccgcgctga ccattgagta agcccgtcat ctggcgcgac cagcttaagt
     7141 aatcctgcgc cagcatcggc aggtcgagca accattcgcc gggataatcc acaatttcca
     7201 gatacagcgt ggaggtatcc ttaaagtggc gcagcagcgc atcgttcgat ttaaaacgta
     7261 acgccaggcg aatttcactg acgccgcgcg taggcgtcgg ccaggcggga ggatcaccat
     7321 ataactgcgc cagtccttca tcataggtga agcgcggaat gccaaagtca cgctggggaa
     7381 tgcgttttac gcccagcaag cgctcttcac gcaccgcgct taacagcggc aaacgtgctc
     7441 cggcatgaat attgagcaac tgattaacca tcgcggtgat aaacgctgtt ttgccgctgc
     7501 ggctaagtcc ggttacagcg aggcgcagat gtctgtcgac accccgattc accagcgcat
     7561 tgagttcatt tttaagtcgc ttcattgctg tcctgtgctg ctctgaagac cagaaaaaaa
     7621 atcattcgta aggatatgaa tgtgggggcc aggccattga ttatcaactg cccgtgttgt
     7681 catcgacggg cgaactccat aagatgtgca gcattcacgt tcggacggag ttcctgacct
     7741 atgtccccta ctatttatga tattgccagg gttgcaggcg tatcaaaatc caccgtatca
     7801 cgcgtgctga ataagcaaac taatatctcc ccggaagcgc gcgaaaaagt gttacgggcc
     7861 attgaagaat tacagtatca gccaaacaag ctggcccgcg cgctgacctc ttcgggtttt
     7921 gatgccatta tggtgatttc tacccgttcg accaaaacta cggcgggtaa tccgtttttc
     7981 tctgaagttt tgcatgccat caccgccaaa gcggaagaag aaggtttcga cgtgatatta
     8041 caaacgtcgc acaatccggc agaagatctg caaaaatgcg aaagcaaaat taaacagaaa
     8101 atgattaaag gcattattat gctcagttcg ccagcggatg agtcattttt tgcccaactc
     8161 gataaatacg atattccggt ggtggtaatt ggcaaagttg aaggtcaata tgcccatgtt
     8221 tattctgtcg ataccgataa ttttggtgac agcattgcgt tgaccgatgc attaattgaa
     8281 agtgggcatc aaaatattgc ctgcctgcat gctccgcttg atgtccatgt ttcggttgat
     8341 cgcgttaatg gctataagca aagtctgtcc gcgcataata ttgcagtgcg tgataaatgg
     8401 attgttgacg gcggttatac ccatgaaaca gcactgcaag cagcacggca attattaagt
     8461 cagtcaccgt tgcctgaggc agtgtttgcc actgacagcc tgaaattaat gagcatttat
     8521 cgtgcggcgg cagagaaaaa tattgctatt ccgcagcagt tagcggtggt gggttatagt
     8581 aatgaaacgc tgtcgtttat tttaacgcct gcaccaggcg gcatcgatgt tccgacgcag
     8641 gagttaggtc aacaaagctg cgagttatta ttccgcttaa tttcgggaaa accgtcacca
     8701 cagaatatta ccgttgccac gcatatgacg ctgaaataaa taccagctac cttgcctgaa
     8761 tgccggatgc gatgctggcg catcttatcc ggcctacatt tatagatcgg ataaagtact
     8821 cgcgccgcat ccggcaattt agcccattat cagaacgagt aatttacgcc gacacctgca
     8881 taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc gtattccagc
     8941 gaaacggaaa gtccgttctg gaaatcataa ccgtaaaata aacccacacg gttaaaatca
     9001 tggccttcac gttcaatatc atcctgccag tcccagttac tctagcgatc cagcccaatg
     9061 cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc gcgaatttct
     9121 tgtgtggaaa actcaccgtt attgcggctg tcgtccatat tgaagccgcg ctcgagataa
     9181 tagttcactc gcaaagcaac cgtttcgttg aaggtatatt gcagacctgt ttccgtttcg
     9241 acacgggtat cagcgtaacc ggtcgtgttc aaatcgttgg caaatttata catcgacaac
     9301 caaccgttga aacgtaaatc atcagtcagt ttcacatccc agtctggcgc gattttccag
     9361 cgctgcatat tcgccgtgtc tttgcccggt tcatcaacgt agtgataacc ataattacgg
     9421 aaaccgccgg tcaggccgaa actgaaatca tcgctttcga ggaactgata atgcacctcc
     9481 agctccgggc gatcaaacct cgttccacgt ttacccgcac tataatctac cggcccttcc
     9541 tgataatagg ccagagcaat tctccacggc ccgttggcgg cattaaaata gactgaaggc
     9601 tccgccagcc catccatatc ttcgccataa ccctcgacgt tttctatttc gtacatcgca
     9661 ccgatattaa agtgccagtc gctcttttcc tcggcctgtg cgcaggccat tcccgcacac
     9721 atcaccagtg cggtacaggg taataacttt ttttgttcct tgtattatcc tgggggaatg
     9781 ccttccccct gtatttagcg aattgctatt tccgtttctg catcaaagaa atgacatttc
     9841 gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa cgcaccagca
     9901 cggaccacta actcgtgccc ccaaccgtgg tgtagagcat aaattccgca ccggttaatt
     9961 ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga tgaatatctt
    10021 ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc agaaccgcta
    10081 atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg ccatcgatcg
    10141 tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac atattcgcgg
    10201 gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct ttcataatca
    10261 caatccgcgc cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg
    10321 tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc agcttggcat
    10381 caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc
    10441 cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact
    10501 cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga
    10561 ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg
    10621 cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca ttcattcgtt
    10681 tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg
    10741 tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta tcggcaattt
    10801 ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta
    10861 acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc
    10921 gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa gcccgcaccg
    10981 atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca
    11041 atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag aaagatttcc
    11101 ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc
    11161 agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag ccctaccgga
    11221 atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac agcattaacc
    11281 gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag ttgcgcccgc
    11341 tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca
    11401 tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat gccaatttca
    11461 gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc
    11521 agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta gcggtcccat
    11581 tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct
    11641 gaccgtccaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcga
    11701 gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct
    11761 caagtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct
    11821 gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca tcacaactat
    11881 gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca
    11941 gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa
    12001 tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag aactgctctg
    12061 gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc tcgttcactt
    12121 ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg tatttcgcca
    12181 gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg tcgggctgaa
    12241 tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac gcgtcgtcac
    12301 tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac accatatagc
    12361 tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata acatcatgaa
    12421 tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa
    12481 gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa
    12541 taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt
    12601 gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct tcgccgctgc
    12661 gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg
    12721 cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa
    12781 acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt
    12841 cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc gtcatgatgc
    12901 gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg ccatttaccg
    12961 tgatacggcg tttctgccac cattgacgcc agttttctgt tgatgctgca agatgttggt
    13021 cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc cactcgtcta
    13081 aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct
    13141 cgcctgcatg aagctgcgta ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc
    13201 attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca ctgcgcccat
    13261 cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt
    13321 cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa atcagcactg
    13381 acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc agttggtctg
    13441 ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta cgccagacaa
    13501 cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata
    13561 acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc agattcacca
    13621 gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg
    13681 tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg
    13741 ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta
    13801 ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata acgccgccac
    13861 cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac
    13921 gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca tttcccgttg
    13981 catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc
    14041 accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga tgttgagtgc
    14101 aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga
    14161 atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt
    14221 gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc ccagcacata
    14281 catcgcagca tccagcatat gaatgccgat gtcgatcagc aggccaccac cctgcagttc
    14341 tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt
    14401 aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag
    14461 cgcaaacgat ggtgaaagtc gtaggccagc accttgccca gtttgcgcgc ggtatcgcac
    14521 atttcccacg cctgttctgt cgtcatggcg ggcggttttt cgcacatcac atggcagcct
    14581 gcttccagtg ccatcagggt atgttcgtaa tgaaaacggt taggtgagcc gacgctaacc
    14641 acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacacgga tgcattcccg
    14701 tatttttctg ccagcgcctg cgcctgggaa aggtggctgt cacagacagc caccagttcc
    14761 agatcgttgc gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcccgcgcct
    14821 attatcgcga cccgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag
    14881 gtacgcaacc aggccaacga atcacggtac gcctgggcag gatcttctgc ccggatacgc
    14941 ccttcataca ccacataacc ctgatagtta tcggcgcgca gctgttcaaa cagcgcgtgg
    15001 aaatcgaggg tgccactgcc cggctggtaa cgatggttat cggcaatatg cacatgaccg
    15061 agcaagtcac ggttgtcgtg cagcgcctgc gccaggttat cttcttcgat gttcatgtga
    15121 tagaaatcgc cgataatctg cacatgttta agattgtttt cgacgatgta acgacgagca
    15181 tcggcgaggg tgttgatcat atgatcctga tagcggttta acggttcgag atacaccacg
    15241 gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cagtttgcgg
    15301 tcgccgtcca ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac
    15361 gctgccggaa cccccccact tctgccagcg cctcgagaat gcgttcgatc tgctttaagc
    15421 cattaagacg acgctcttcg ataaagtcac caatccagcc gtcatagccg ccgcaggcgg
    15481 tggtcactgg taaaccggtt tctttgatcg ccgctttgac ttcttcgatg ttgttgacca
    15541 gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatctct
    15601 cgcggatatt ttccggaaag aatgcctgat tctgtgtgcc gattttcatt ttttcattcc
    15661 tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg
    15721 catgtagctt tccgggctgt tggcaaaggt cactaccggg tcgattaaat cttcgcaatt
    15781 gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg
    15841 cggataatcc gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa
    15901 atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacatagga
    15961 gatggtgccg ccgtaggcca ggccgcgcag cgccgattgc agcgcgtcgg cgtaaccgct
    16021 ggtttcgata atcacatcag ccccctgctt gccggtcagc gttttgatct ctttgcctac
    16081 gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg
    16141 atgggcgata ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg ccagttggat
    16201 ggcaatttga ccgatcgcgc caagccctac caccacgaca aaatccccta cgcgtacgtt
    16261 ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacagacgg cgtttttcca
    16321 ggagctgcct tgcggcattt tgcgcagctt gtagttattc actgcgttaa tgatgaccgt
    16381 ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtctg taacgtcgct
    16441 gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat
    16501 gccgcgcggc gcatctgccg gacgcggggt gaacatctgc cactcgccgt taaagtcttc
    16561 atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc
    16621 gaaacgcacg cggatcttca cttcattggc taaaatcgcc cgatcttcat actcaaccag
    16681 cgcagcaaca cgcggtgctg tggctactaa ctttttcatg taaaactcct tgtaaagaaa
    16741 ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccaa
    16801 aagcgcatac ataatcacca ccggcaatgc ggtcaccagc gaggccgcca tcatccgtcc
    16861 ccagatgtaa tcaggcgtac tgaacagcgc gttcaggccc actggtaagg tgaaattgct
    16921 ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta
    16981 gacgaacacc gatatcagcc cggacatcgc caggggcacg gtgatacgga agatgatttg
    17041 cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcaggga tggtgtcgaa
    17101 gtaacttttc agcataaaca cggcggtggg cagggtttgc gtcaccatgg taatgatcag
    17161 cgccatctcg gtgtcgtaaa taccaagcgc ggtgatgatt ttgaacagag gcaccaccag
    17221 caaaatgcca gagaacatat acaccgtgta aaagctggcg ttgatggtca tccggccttt
    17281 aaagcgcagg cgggaaagcg cgtaagctcc gagaatgccg agaaataccg ccaccacgga
    17341 ggaaaccacc gacaccacca tgctgttacg gaagtagtcg acaaacggga aaatcatcgg
    17401 gttaaaaatg tcgacgtaat gctccagcgt ccactgttgc ggcagcagcg taggatgcag
    17461 cgatatcgcc tctttcgcgc ttttgaacga ggtcatcagc atcacaaaaa acggaaacag
    17521 cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atacgactga gcgtgcgttt
    17581 atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga
    17641 tgaagagcac caccgagatc gccgccgctt tccctaagtc attaaaggca aaggcggttt
    17701 tgtagagata gacaccaaga atatcgacct tggttgtcag cagataaaca tcagcgaaca
    17761 tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataattgcgg
    17821 gcagcgtgac gatgcgaaac cgctgccagg cattagcgcc atccatctct gccgcttcgt
    17881 acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt
    17941 agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta tcaaaccaca
    18001 gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc
    18061 cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga
    18121 ggatcaccag cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa aacatcgcca
    18181 cggccagccc gagcacggtg ctgcccgcca ccaccagcgc cgtataccag accgtcatcc
    18241 acagcgagtg ccagaagccg ggatcggaga gaatgcgcac atagttactc accccaacaa
    18301 acgttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca
    18361 ccatcggcca cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcagcagcg
    18421 caaacggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt
    18481 ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag
    18541 atcggcttta cccacggtga cgttatgcac catggagctc accacaccag aacccgtcac
    18601 atcgcccatg cgggtaaagt ttttatcccc tactgcgcca aagacctgaa tatttggcag
    18661 ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca
    18721 ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac
    18781 ccagtcggca atgttgtccg cctgctccat aaaggtgaca aatttttctg ctgcttcggt
    18841 cttttgtccg gcggtaatgg tcagcgaggt caacatgccg tagaccgcag agtttttctc
    18901 ggttggcacc acgaaaccga cgtttttcgg gtcgccttct ttaatcacag ccggaaggat
    18961 ataggtggag taaatcgcca tcggcgcggt gccgttcata aaggcgtctt tcacttccat
    19021 gatgtcgtta gaacccggca tggtgttggc agcaaggtcg cggtaatagg tcagcgcctg
    19081 catcatctct ggtgtatcaa gggtgatttt gccttcggcg ttaaagacgt tggcctagtt
    19141 ggataacgca aactgggaga aggattgttc cgtcagcacg ctttctgccg taggcagcgc
    19201 aataccgtat tttttattcg ccgggtcatt cagtttctgt gcaacgtcca gcagctgttg
    19261 ccagtttttc ggctcctcca gccccgcttt tgccagcaca tctttgcgat accagatgcc
    19321 gccaatccag gcgctgacag gaacaccggt ccatgcgcta ccatcttcgg tacgcacaat
    19381 acgcagtacg ccatcgtaaa acgcgccttc accaacgttg ctgatgaccg tggcaaccgc
    19441 tttgcgatcg ataagctgct ctttatccat aactttggcg tagtcatggc tggtttcgat
    19501 cacttccggc agcgagccgc tacgtgaaag ggtgatgact ttggtgttat gagcgtcttc
    19561 ttccacgggc acttgtttaa cggtgatacc tgggttttct ttttcaaaac gggcaatcaa
    19621 tttgctgata actgcctgac gctcctgctc caccgaagaa tgcataaatt cgatggatac
    19681 atgggtttta ttttcttctt tacagcctga aatcagggcg caggaaacca gtgctgataa
    19741 cagcacgatt tttgatttaa tcatgagatg ttccttttta tttaatccac ataacctgcc
    19801 aggggcgcag cgttatttca ttacccaata tatcaacttc actaatcaga tcccgaccat
    19861 gcagatcagt aatatttaca tgctgaatat ttttactgac attaaacaat ccagtcagac
    19921 aattaccatc agcgttactt ctttgaatac gcattacgga tgaattaacc gtatcgatgg
    19981 taaaattatt atccggatga aactcgttat ggctgcgacg aagtgtaatt aaacgcgaca
    20041 actcatgata taccgcatgc cttaatgtag cttcatcgtt cagttctcgt gttatctctt
    20101 cactgtgata ttttttacgg ttaatcgcac ggttatatcc gagtttttcg acacctgcgt
    20161 aatcattacg cgagccaaga ata
//
LOCUS       DAJVPT010000073        20163 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVPT010000073 DAJVPT010000000
VERSION     DAJVPT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            19..171
                     /gene="hokA"
                     /locus_tag="OQM47_002607"
     CDS             19..171
                     /gene="hokA"
                     /locus_tag="OQM47_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1480709.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(358..570)
                     /gene="cspA"
                     /locus_tag="OQM47_002608"
     CDS             complement(358..570)
                     /gene="cspA"
                     /locus_tag="OQM47_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1480710.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(851..1141)
                     /locus_tag="OQM47_002609"
                     /pseudo
     CDS             complement(851..1141)
                     /locus_tag="OQM47_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1575..2285
                     /locus_tag="OQM47_002610"
     CDS             1575..2285
                     /locus_tag="OQM47_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1480711.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2335..3309)
                     /gene="ghrB"
                     /locus_tag="OQM47_002611"
     CDS             complement(2335..3309)
                     /gene="ghrB"
                     /locus_tag="OQM47_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1480712.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3413..4063)
                     /locus_tag="OQM47_002612"
     CDS             complement(3413..4063)
                     /locus_tag="OQM47_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1480713.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4226..6559
                     /gene="bisC"
                     /locus_tag="OQM47_002613"
     CDS             4226..6559
                     /gene="bisC"
                     /locus_tag="OQM47_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1480714.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6528..6968)
                     /locus_tag="OQM47_002614"
     CDS             complement(6528..6968)
                     /locus_tag="OQM47_002614"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1480715.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6965..7528)
                     /gene="tag"
                     /locus_tag="OQM47_002615"
     CDS             complement(6965..7528)
                     /gene="tag"
                     /locus_tag="OQM47_002615"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1480716.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7686..8384
                     /locus_tag="OQM47_002616"
     CDS             7686..8384
                     /locus_tag="OQM47_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1480717.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8613..9821
                     /locus_tag="OQM47_002617"
     CDS             8613..9821
                     /locus_tag="OQM47_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480718.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10145..11836
                     /gene="eptB"
                     /locus_tag="OQM47_002618"
     CDS             10145..11836
                     /gene="eptB"
                     /locus_tag="OQM47_002618"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1480719.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11928..12004
                     /locus_tag="OQM47_002619"
     tRNA            11928..12004
                     /locus_tag="OQM47_002619"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11962..11964,aa:Pro,seq:cgg)
     gene            12748..14355
                     /gene="dppA"
                     /locus_tag="OQM47_002620"
     CDS             12748..14355
                     /gene="dppA"
                     /locus_tag="OQM47_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1480720.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14663..15682
                     /gene="dppB"
                     /locus_tag="OQM47_002621"
     CDS             14663..15682
                     /gene="dppB"
                     /locus_tag="OQM47_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1480721.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15692..16594
                     /gene="dppC"
                     /locus_tag="OQM47_002622"
     CDS             15692..16594
                     /gene="dppC"
                     /locus_tag="OQM47_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1480722.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16605..17588
                     /gene="dppD"
                     /locus_tag="OQM47_002623"
     CDS             16605..17588
                     /gene="dppD"
                     /locus_tag="OQM47_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1480723.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17585..18589
                     /gene="dppF"
                     /locus_tag="OQM47_002624"
     CDS             17585..18589
                     /gene="dppF"
                     /locus_tag="OQM47_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1480724.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18619..19890)
                     /locus_tag="OQM47_002625"
     CDS             complement(18619..19890)
                     /locus_tag="OQM47_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1480725.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4829 a         4993 c         5294 g         5047 t
ORIGIN      
        1 caacacaggg ggctgggaat gccgcagaaa tatagattac tttctttaat agtgatttgt
       61 ttcacgcttt tatttttcac ctggatgata agagattcac tgtgtgaatt gcatattaaa
      121 caggagagtt atgagctggc ggcgttttta gcctacaaat tgaaagagta agagtcttcg
      181 acgggaaatt attcccgcct tacttacggc gttgcgcatt ctcattgcac ccatatttat
      241 tcttcacaaa aataataata gattttatta cgcgatcgat tatttatttc ctgaaaaaaa
      301 ataaaaaaat ccccgccaaa tggcagggat cttagattct gtgcttttaa gcagagatta
      361 caggctggtt acgttaccag ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac
      421 tttctgacct tcgtccagag atttgtaacc atcgttctgg atagcagaga agtgtacgaa
      481 cacatcttta gagccatcgt caggagtgat gaagccgaag cctttgtcag cgttgaacca
      541 ttttacgata ccagtcattt taccggacat agtgtattac ctttaataat taagtgtgcc
      601 tttcggcgat atggcgtgct ttacagattt tgaagcgtta aaggaatgtg cactacgagg
      661 ggtatcaacg ataactcttg aagggacttg ccttactaca ctgctttaat ggtctgtacg
      721 tcaaaccgtt gatgtgcatt aagccactca ttggcgggtg atgcaacaat tatttttcat
      781 atttatgatt aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt
      841 ggataaaagt ctatttcatc aactgcttac ttaatgccgg gttggcttga atcaaacgca
      901 tcaattttag ttcggcactt gaaggcttca cgcgtctgga ttcccattcc tttaccatgg
      961 cgactgatac gcccaaaacc cgggcgaaat catcgatttt taatcctgtc ccttttcata
     1021 actgctcaat ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat
     1081 ctttaaaaac aatctgttcc aggctgctca acagctcatg cattggatct ttatattcca
     1141 ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc atcgaagctc attaagaata
     1201 gtcgggaaaa gcagacacag cggggtgaag tcagggcatg ctctggctat gattcgattc
     1261 agcctgtcat ttagcgtgac ttatagccac ctaacgatct gataaatgcc gatatatctc
     1321 ttatcactcc tgttttgtaa agatttcgca aatctaacct tctgcgaagg cgtaaatctc
     1381 tggctacaaa aaaggcaacg gcactatcat tttttaacaa tgaaacaaca aattaacctt
     1441 gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc ctgcaagcct
     1501 gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg
     1561 aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg
     1621 cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat cagcgtaaag
     1681 cgtttatcga cttcctgcaa aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga
     1741 ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt
     1801 attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggtcgta gacagcgtta
     1861 acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt gaaatgaatg
     1921 gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc
     1981 actctgcgct aaaacagagc gatgacctga aaccggtatt tgatcaggca ttcaccaaag
     2041 tggtaacgac gccagctgat gcattgcaac cgttgattcc ggcggcacaa actttcacgc
     2101 aacaactggt gatggttgga gactacattg ctcagcaagg tactcaggtg agctttgtcg
     2161 caaatggcat ccagttcccg acttcacagc aggcaagtga atataacaaa ctgattgcgc
     2221 cattaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt actgcaaccc
     2281 aataaagagt aaaagcccga gcgattatcg ggctttactt cacgcagtcg cagcttagtc
     2341 cgcgacgtga ggattcacac agttcttctc aacctttcct tgtaacgcat caatcaaatt
     2401 atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc caatatgcgg
     2461 tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggacagtg gctcttgttc
     2521 gaagacatcc agcccggcgg cgtgaatttc gcctttctgc aatgctgcga tcagtgcatt
     2581 ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggaggatt tcattttggc
     2641 gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac
     2701 gaaatctgac tcttgtaaca gagtatccaa atcgcagtag cgggcgttga agcgttcttc
     2761 tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc caaagtgcac
     2821 acgttgtgcc agcgccatgc cgatccgtcc catcccgaca atgcccagtg ttttatggtg
     2881 aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg cttttacccg
     2941 ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca gcgtatcggc
     3001 gacggtttct gttaatacgg ttggcgtgtg catcagcaga atttttcggg cggtaagcgc
     3061 atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca gtttcggcat
     3121 tttttccagc aatgcggcat ctacattctc gtttgaaccc agtaaacctt cagcttctgc
     3181 aaaaattgct gcattttgaa cgacggtttg tgggctgagg tttgccacct ggtgaacggt
     3241 gaaatgcgct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga ggataacgga
     3301 cggcttcatg cttctctcca ttcactgata aggcgatcac cttagcctga aatatgatga
     3361 aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg gattacagcg
     3421 ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg
     3481 cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc acgccctggg
     3541 tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg ttcaggtcgt
     3601 gaccccccgt gctgtcggta taaccaatca cgttaaccgc cgttttcgga tactctttca
     3661 gcaccattgc cacgccggtc agggtgttag cgcctgccgg tttcagggga gcgctgctgc
     3721 tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg cgggttacgc
     3781 taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac
     3841 ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat
     3901 ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagccc agacctgcgc
     3961 cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg gttagttgtg cagccagata
     4021 ccgccagagc gccactcact acggcggcaa gtaagataaa cacgtttctt cattgttaat
     4081 ccttaataac ctttttattc tttgtcacgg gttccgtggc gggagattat gccgcgtgaa
     4141 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     4201 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
     4261 tgccgcccac tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
     4321 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     4381 cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc tttctcgcgt caccggaaaa
     4441 cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     4501 tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     4561 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     4621 ctatatggcg ctggcaggcg gttataccgg gcatctgggg gattattcga ccggtgcggc
     4681 acaggcgatc atgccgtatg tcgtaggtgg cagcgaagtt tatcaacagc aaaccagttg
     4741 gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac
     4801 gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg
     4861 tgacagtggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg tcgatttctt
     4921 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg
     4981 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac
     5041 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     5101 cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc
     5161 tattttccac caaaatacca ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt
     5221 tggtgagcaa aaacactgga tgatcgtcac gctggcagca atgttggggc aaatcggcac
     5281 acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcggcg
     5341 ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg cggtggataa
     5401 aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa
     5461 cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg gtgccaactt
     5521 tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt
     5581 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatattgttc tgcctgcgac
     5641 tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     5701 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
     5761 cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca
     5821 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     5881 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
     5941 cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc
     6001 gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg attacggtta
     6061 cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
     6121 accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca gccagctgaa
     6181 ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc
     6241 tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt ggaactcccg
     6301 tgggcagatc cttgccggag cggtcattag cgagggaatt aaacctggcg tgatttgcat
     6361 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
     6421 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     6481 tacgacgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
     6541 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     6601 acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt ttgataaacc
     6661 tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt accaataccg
     6721 cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     6781 acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag
     6841 gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa gggatgcccc
     6901 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc
     6961 cgaatcatgg tttatttccc agatagcaac agcagccaac cacatgatca ttcaccagcc
     7021 cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa ccacgttttt
     7081 tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg
     7141 tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg ggaaacggtt
     7201 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     7261 ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     7321 ctgcgacctt caccggatcg aactgatgaa agtaggcgcg atagttttcg cgttttttga
     7381 ggacggtgat ccacgataac ccagcctgct gcccttcaaa gcagatcatt tcgaacagtt
     7441 ttttactgtc agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggc
     7501 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     7561 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     7621 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     7681 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagcgtgg
     7741 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     7801 cgcaacccgg acattccaca gagcgttaca cctgggatag tgatcatcaa cctgattaca
     7861 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     7921 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     7981 tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
     8041 cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca
     8101 gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     8161 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     8221 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     8281 tgctcaatca aaatattgcc gcctatgctg cgctaactca ggcagaaaat accactaata
     8341 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt
     8401 gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca aataataaat
     8461 ttggtgaata attgtcgcgt tattcattcc tgaactaagg catttcattc cgttctgatg
     8521 gcatttcatg ccgtttttcc ccaagcataa aatgcacttc gttatggttg tcggcagaga
     8581 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
     8641 gctggctgac actcatcggt actatcatta cccagtttgc gctggggtcg gtttataccc
     8701 ggagcctgtt taatggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt
     8761 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     8821 aggaacgttt tggcgttaaa cgcgtcaccg tggcttccgg cattttgctg ggattaggct
     8881 tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
     8941 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
     9001 cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
     9061 gtttcaaatt tatcgacacg cagctgctcg aaacggtggg tctggaaaaa acctttgtga
     9121 tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg aaagacgcac
     9181 caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg ctggcagagt
     9241 cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgactgcc tgcatgagcg
     9301 gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac cttgatgtgg
     9361 tttccgcagc caatgcagta acggttattt ccatcgctaa cctttcaggt cgtctggtgc
     9421 tgggcattct gtctgacaaa attgcccgta tccgtgttat caccattggt caggtgattt
     9481 cgctggtggg tatggcggcc ctgctgtttg caccattaaa tgcagtgacg ttctttgcag
     9541 cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttcca tcactggtca
     9601 gtgagttctt cggcctcaat aacctggcga aaaactacgg tgtgatttac ctcggtttcg
     9661 gtatcggtag catttttggt tcgattatcg cctcactgtt tggcggtttc tatgtgacct
     9721 tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgacg attcgtcagc
     9781 cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc gcgagctact
     9841 gggcttcagc ttccgtgtgc atcaaacgca tcaacgaaat gtataaacaa tgactcgtaa
     9901 aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag tctcttttca
     9961 aacttgcatt tttgtaaatt tgtgcttcat gcacactctt tccccacact ttttcgcttt
    10021 gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc
    10081 ccctgccggg cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc cagggtttgt
    10141 ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta
    10201 tattggcctt tttatgaatg gcgcggtttt ttatcgccgc ttcggcagct atgcgcacga
    10261 ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg gccgccaccg tactggtgac
    10321 cttcttttta ctacgtcttc tatcgctgtt tggccgccgc agctggcgta ttctggcgtc
    10381 gctggtggtg ctcttttccg caggtgccag ctattacatg accttcctta atgtggtcat
    10441 tggttatggc atcatcgctt ccgtcatgac caccgatatc gacctgtcaa aagaagttgt
    10501 tggtctgaac tttattctct ggttaatcgc cgttagtgca ttgcctctta tccttatctg
    10561 gaataaccgc tgtcgctaca ccttgctccg acaactgcga accccagggc agcgtattcg
    10621 cagcctggtg gtcgtcgtac tggcgggtat tatggtttgg gcaccgattc gtttgctgga
    10681 tatccagcag aagaaagtgg agagggcgac cggcgttgat ttgccgagtt atggcggtgt
    10741 cgtagcgaac tcttatctgc catcaaactg gctttctgcg ttggggctgt atgcctgggc
    10801 gcgggtcgat gaatcttccg ataataattc attgcttaat ccggcgaaga agtttacata
    10861 ccaggctcca caaaacgttg atgatactta tgtcgtgttt atcatcggtg aaaccacgcg
    10921 ttgggaccat atgggtattt tcggctatga gcgtaatacc acgccgaaac tggcccagga
    10981 gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac tctcactgcg
    11041 ttgcatgttt gtacgtcagg ggggcgcgga agataatccg cagcgcacat taaaagaaca
    11101 gaacattttc gcggttctga agcagttagg attcagttct gacctctacg ctatgcagag
    11161 cgaaatgtgg ttctacagca acacgatggc agacaacatt gcttatcgtg agcagattgg
    11221 tgcggagcca cgtaatcgtg gcaagccggt agatgatatg ttgctggtag acgaaatgca
    11281 gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggttc
    11341 gcattttaac tacacccagc gttatccgcg tagcttcgcg cagtggaagc cggaatgtat
    11401 tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcctatgaca actcggtgac
    11461 ctatgtggat cactttatct ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt
    11521 gttctacgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc
    11581 gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg atggtctgga tgtcagataa
    11641 atatctggaa aatccggcca atgcgcaggc gtttgcgcag ctgaaaaaag aagccgacat
    11701 gaaagtgcca cgccgtcacg tagagctgta cgacaccatc atgggttgtc ttggctatac
    11761 ttcaccggat ggtggaatta acgaaaacaa caactggtgt cacatcccgc agacaaaagt
    11821 ggcagcggct aactaagcgg cctgctgact ttctcgccga tcaaaaggca ttttgctatt
    11881 aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg tgattggcgc
    11941 agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg
    12001 accaaattcg aaaagcctgc tcaacgagca ggtttttttg catttgtagc tcatgaggat
    12061 gagaacctcc gggggcaggg aggctcgact cgacgtaggc ctgataagac gcgccagcgt
    12121 cgcatcaggc aacggcaccg cacttattgt cggatgcggc gcgaccgcct tattcgacca
    12181 acacaccaca cattttcctg ccaactttgc gccccatatc tcactatcca ttaacggatt
    12241 tgtgacagaa accattgtct ttttttatat ttgcttaaat cttttttgcg ttacttatgg
    12301 ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc
    12361 caccttttcc tcagattttt ttcattaaat gcataaaact taatcaccaa atgttgctaa
    12421 atattaagct gattgttctg gttatgttgg agtagcacaa atttctctgc tgcaaatagc
    12481 tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa atcccggttg
    12541 gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg cattttatgg
    12601 aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca taacgttgac
    12661 ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac
    12721 aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc agggatgctg
    12781 aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg
    12841 gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac ctccggcacc
    12901 acctatgacg cctcttccgt cccgctttat aaccgcctgg ttgaatttaa aatcggcacc
    12961 accgaagtga tcccgggcct cgctgaaaag tgggaagtca gcgaagacgg taaaacctat
    13021 accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa accgacgcgc
    13081 gaaatgaacg ccgatgacgt ggtgttctcg ttcgatcgtc agaaaaacgc gcaaaacccg
    13141 taccataaag tttctggcgg cagctacgaa tacttcgaag gtatgggctt gccggagctg
    13201 atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac tcgaccggaa
    13261 gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct
    13321 gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat cggaaccggt
    13381 ccgttccagt tacagcagta ccaaaaagat tcccgtatcc gctacaaagc gtttgatggc
    13441 tactggggca ccaaaccgca gatcgatacg ctggtcttct ctattacccc tgatgcttcc
    13501 gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga tgccgtaccc gaacccggca
    13561 gacatcgctc gcatgaagca ggataaatcc atcaacctga tggaaatgcc ggggctgaac
    13621 gtcggctacc tctcgtataa cgtgcagaaa aaaccgctgg atgacgtgaa agttcgccaa
    13681 gcactaacct acgcggtgaa caaagacgcg atcatcaaag cggtttatca gggcgcgggt
    13741 gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga cgttcaggat
    13801 tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt
    13861 ttctccatcg acctgtgggc gatgccggta caacgtccgt ataacccgaa cgctcgccgc
    13921 atggcggaga tgattcaggc agactgggcg aaagtgggcg tgcaggccaa aatcgtcacc
    13981 tacgaatggg gtgagtacct caagcgtgcg aaagatggcg aacatcagac ggtaatgatg
    14041 ggctggactg gcgataacgg ggacccggat aacttcttcg ccaccctgtt cagctgcgcc
    14101 gcctctgaac aaggttctaa ctactcaaaa tggtgctaca aaccgtttga agatctgatt
    14161 caaccggcgc gtgctaccga cgaccataac aaacgcgttg aactgtacaa gcaagcgcag
    14221 gtggtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt gtttgaaccg
    14281 gtacgcaaag aagtcaaagg ctatgtggtt gatccattag gcaaacatca cttcgaaaac
    14341 gtctctatcg aataattaaa agccatacaa gactgatggc aaaggcaaaa ctgcctgatg
    14401 tgctccgctt atcaggccta cgaaaattct gcaatgtatt gaatttgcac gattttgtag
    14461 gccggataag gcgttaacgc cgcatccggc ataaacaaag cgcactttgt caacaatctg
    14521 tatacccggt ggcgctgtgc ctctgcgtgc agcgccatcc ggcagcaata cttcattctc
    14581 tgtccgacaa gggcgggaat tgatttgtga gcaatacaga cacgcagttc caggctgcga
    14641 gtcactatag agaatccggg ttatgttgca gtttattctc cgacgtttgg gactcgtcat
    14701 ccccacgttt atcggtatta cccttctcac atttgccttt gtccacatga tcccgggcga
    14761 tccggtgatg atcatggcag gcgaacgtgg gatctcccct gagcgtcacg tgcagctgct
    14821 ggctgaactc ggcttagata aaccgatgtg gcagcagtat ctccattaca tttggggcgt
    14881 aatgcacggc gatctaggca tttcaatgaa aagccgaatt ccagtatggg aagagttcgt
    14941 gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg atgatttttg ctacggcagt
    15001 cggtattccg gttggtgtgc tggctgcggt taaacgcggt tccattttcg atcacactgc
    15061 agttggcctg gcgctgaccg gctactcgat gccgatcttc tggtggggca tgatgctgat
    15121 catgctggtt tcggtgcact ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt
    15181 gttcctcgat gactccaatc cgttaaccgg ttttatgcta atcgacaccg ccatctgggg
    15241 tgaagacggc aactttatcg atgccgtcgc ccatatgatc ttgcccgcca ttgtgctggg
    15301 tactattccg ctagcggtca ttgtgcgtat gacacgctcc tcgatgctgg aagtgctggg
    15361 cgaggattac atccgcaccg cgcgcgccaa agggctaacc cgcatgcggg tgattatcgt
    15421 ccatgcgctg cgtaacgcga tgctgccggt agtgaccgtt atcggcctcc aggtgggaac
    15481 attgctggcg ggggcgattc tgaccgaaac catcttctcg tggcccggtc tggggcgctg
    15541 gttgattgac gcactgcaac gccgcgacta tccggtagtg cagggcggcg tattgctggt
    15601 ggcgacgatg attatcctcg tcaacttgct ggtcgatctg ctgtacggcg tggtgaaccc
    15661 gcgtattcgt cataagaagt aaggggacat catgtcacag gttactgaaa ataaagtgat
    15721 tagcgcaccg gtgccgatga ccccgttaca ggagttctgg cactatttta aacgcaacaa
    15781 aggcgcggtc gtcgggctgg tttacgtcgc catcgtgctg ttcatcgcga tctttgccaa
    15841 ctggattgcg ccttataacc cggcggatca gttccgcgat gcgctgctcg ccccgccagc
    15901 ctggcaggaa ggcggcagta tggcacactt gctgggcacc gatgacgtcg gtcgtgatgt
    15961 gctgtcgcgg ctgatgtacg gcgcgcgttt atcgctgctg gttggctgtc tggtggtggt
    16021 gttgtcgctg attatgggcg ttattctcgg cctgatcgcc ggttacttcg gcggcctggt
    16081 tgataacatt atcatgcgcg tggtcgatat catgctggcg ctgccaagtc tgctgttggc
    16141 actggtgctg gtggcgattt tcggcccgtc gattggtaac gccgcgctgg cgctgacctt
    16201 cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc gtgctggtgg aggtgaatcg
    16261 cgattacgtc accgcctctc gcgtggcagg tgccggggcg atgcgccaga tgtttattaa
    16321 catcttcccg aactgccttg cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc
    16381 cattctcgat atggctgctc ttggtttcct cggcatgggg gcacagccgc caacgcctga
    16441 gtggggcacc atgctctccg acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac
    16501 cttccctggt ctggcgatcc tgttgacggt gctggcattt aacctgatgg gtgacggtct
    16561 gcgtgacgcg ctcgatccca aactaaagca gtaagaggtt cgagatggcg ttattaaatg
    16621 tagataaatt atcggtgcat ttcggcgacg aaagcgcacc gttccgcgcc gtagaccgca
    16681 tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta
    16741 agtcggtcag ttcactggcg attatggggc tgattgatta tccgggccgc gtaatggcag
    16801 aaaaactgga gtttaacggc caggatttgc agcgtatctc agaaaaagag cgccgcaacc
    16861 tggtgggtgc cgaagtggcg atgatcttcc aggacccgat gaccagcctt aacccgtgct
    16921 acaccgtggg tttccagatt atggaagcga ttaaagtgca tcagggcggc aacaaaagta
    16981 cccgccgtca gcgggcgatt gacctgctga atcaggtcgg tattcccgat ccggcttcgc
    17041 gtctggatgt ttacccgcat cagctttccg gcggcatgag ccagcgcgtg atgatcgcca
    17101 tggcgattgc ctgtcggcca aaactgctga ttgccgatga accgaccacc gcgctggacg
    17161 tgaccattca ggcgcaaatc atcgaactac tgctggagct acagcagaaa gagaacatgg
    17221 cgctggtgtt aattacccat gacctggcgc tggtggcgga agctgcgcat aaaatcattg
    17281 tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca cgccatcttc catgcgccgc
    17341 gtcacccgta tactcaggca ttgctgcgtg cgctgccaga atttgctcag gacaaagaac
    17401 gtctggcgtc gttgccaggt gttgttcccg gcaagtatga ccgcccgaac ggctgcctgc
    17461 ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata
    17521 tgctcgctga tgggcgtcag tccaaatgcc attacccact tgatgatgcc gggaggccca
    17581 cactatgagt acgcaagagg ccacctcgca acaaccgctg ttgcaggcta tcgacctgaa
    17641 aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa cgtctggtga aggcgctgga
    17701 cggcgtttcg tttaaccttg aacgtggcaa aacgctggca gtagtgggcg aatctggctg
    17761 cggtaaatcg accctcggtc ggttgctgac gatgatcgaa acgcccaccg gcggcgagct
    17821 gtattatcag gggcaggatc tgctcaagca cgatccgcag gcgcagaagc tgcgtcggca
    17881 gaaaatccag atcgtcttcc agaatcccta cggttcgctg aatccgcgta agaaagttgg
    17941 gcaaattctc gaagagccgc tgcttattaa caccagctta agcaaagaac agcgtcggga
    18001 aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc gagcactatg accgctatcc
    18061 gcacatgttt tccggcggac aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga
    18121 cccggatgtg gtgattgccg atgagccggt ttctgcgctg gacgtctcag tgcgtgcgca
    18181 ggtgctgaat ctgatgatgg atttgcagca ggagttgggg ctttcttatg tctttatctc
    18241 ccacgacctg tcggttgtgg agcacattgc tgatgaagtg atggtgatgt acctaggccg
    18301 ctgcgtggag aaaggaacga aggaccaaat cttcaataac ccgcgccatc cgtacaccca
    18361 ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat cgtcgtgaac gcatcaaact
    18421 caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt tgcgccttca acgcccgctg
    18481 tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag ctaaaagact acggaggtca
    18541 actggtagct tgttttgctg ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc
    18601 ggggccatgc gctccggttt actcaatcag cttcaacagt ggcgacaagc atagcaaaat
    18661 gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt
    18721 atagatcagg aaaaacggaa tgaggcacga cacaatgcca tataacgggc ttcccaactg
    18781 aaagaacacc agcactgata cacgaaacga aacccaaatc gtcaacgtaa tgacgataaa
    18841 agcgcagatc gccagagtca gcatgcgtga gttaattttc ttggtatcaa taattcggct
    18901 taacagattg agaataatgc ctttaatagc ctcgtggaaa ccgagataaa tgccaaagaa
    18961 tgcggtcagt acggcaaaga tattcagcac cgtagaggtg atatgaatga tatgtccagg
    19021 gatcacctgc gcggccagtg ccagtgccga gatattttgt tcaaaggcag aaacggcttc
    19081 ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac aggatcaccg cgatgagcgt
    19141 gatataacta attcggtggg tacgcagcgc gagccgggtt gccagtactt tatctgcttc
    19201 ccgtttacga taggcaatat tcattgggtt aagtacctga ataaataccg cagaaaagaa
    19261 gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt
    19321 tatattggcg aaattccagt gcgggatcat cgcaaaaccg aacacgacaa taatccctac
    19381 tttgaccacc accattggcc cagaaatctt aaacagtaat cgttcaccac cagacgcaat
    19441 cgctatcagt acggcgaaaa tagcgacttt ataaagtaga gattgtgaaa gatcggcatc
    19501 ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa accacggaga gagagtagat
    19561 aaaaatcccg tggataatca tcaaaaagta gataaccccg aggaaaattc cccagttctt
    19621 ccccaggtaa tgactgataa tatcggtgta gtcattacag gaatcgcttt cagaaagggt
    19681 ttttaaataa atgtcctgca ctacccaggt gacaggataa gcaatgatcg ctgcggtaat
    19741 aaatacccaa attcccttca agccaatttg tactggcatc agcacggttc cggcaccaat
    19801 cgccatgcct atgcataata aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg
    19861 attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg
    19921 caaaaatgaa taaacccagt ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat
    19981 gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat
    20041 tgcgcatttc actaccagtc tggagtcagc ccggatatga actgcaacta ttgcaaataa
    20101 agaaattaag ctggaaggca tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa
    20161 gca
//
LOCUS       DAJVPT010000074        20109 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVPT010000074 DAJVPT010000000
VERSION     DAJVPT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..437)
                     /locus_tag="OQM47_002626"
     CDS             complement(<1..437)
                     /locus_tag="OQM47_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1480726.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIY"
     gene            complement(492..1343)
                     /gene="manZ"
                     /locus_tag="OQM47_002627"
     CDS             complement(492..1343)
                     /gene="manZ"
                     /locus_tag="OQM47_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1480727.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1356..2156)
                     /gene="manY"
                     /locus_tag="OQM47_002628"
     CDS             complement(1356..2156)
                     /gene="manY"
                     /locus_tag="OQM47_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1480728.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2219..3190)
                     /gene="manX"
                     /locus_tag="OQM47_002629"
     CDS             complement(2219..3190)
                     /gene="manX"
                     /locus_tag="OQM47_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1480729.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3653..5203
                     /gene="yoaE"
                     /locus_tag="OQM47_002630"
     CDS             3653..5203
                     /gene="yoaE"
                     /locus_tag="OQM47_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1480730.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5207..6805)
                     /locus_tag="OQM47_002631"
     CDS             complement(5207..6805)
                     /locus_tag="OQM47_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1480731.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6936..8300)
                     /gene="sdaA"
                     /locus_tag="OQM47_002632"
     CDS             complement(6936..8300)
                     /gene="sdaA"
                     /locus_tag="OQM47_002632"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1480732.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8484..9062)
                     /locus_tag="OQM47_002633"
     CDS             complement(8484..9062)
                     /locus_tag="OQM47_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1480733.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9066..10427)
                     /gene="pabB"
                     /locus_tag="OQM47_002634"
     CDS             complement(9066..10427)
                     /gene="pabB"
                     /locus_tag="OQM47_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1480734.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10501..10680
                     /locus_tag="OQM47_002635"
     CDS             10501..10680
                     /locus_tag="OQM47_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1480735.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10800..11159)
                     /locus_tag="OQM47_002636"
     CDS             complement(10800..11159)
                     /locus_tag="OQM47_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1480736.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11521..11865)
                     /locus_tag="OQM47_002637"
     CDS             complement(11521..11865)
                     /locus_tag="OQM47_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1480737.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11997..13907
                     /locus_tag="OQM47_002638"
     CDS             11997..13907
                     /locus_tag="OQM47_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1480738.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13965..14660
                     /gene="tsaB"
                     /locus_tag="OQM47_002639"
     CDS             13965..14660
                     /gene="tsaB"
                     /locus_tag="OQM47_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1480739.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14700..15281
                     /gene="yeaY"
                     /locus_tag="OQM47_002640"
     CDS             14700..15281
                     /gene="yeaY"
                     /locus_tag="OQM47_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1480740.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15487..17172
                     /gene="fadD"
                     /locus_tag="OQM47_002641"
     CDS             15487..17172
                     /gene="fadD"
                     /locus_tag="OQM47_002641"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1480741.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17254..18369
                     /gene="rnd"
                     /locus_tag="OQM47_002642"
     CDS             17254..18369
                     /gene="rnd"
                     /locus_tag="OQM47_002642"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1480742.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18423..19388)
                     /gene="yeaX"
                     /locus_tag="OQM47_002643"
     CDS             complement(18423..19388)
                     /gene="yeaX"
                     /locus_tag="OQM47_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1480743.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19444..>20109)
                     /gene="yeaW"
                     /locus_tag="OQM47_002644"
     CDS             complement(19444..>20109)
                     /gene="yeaW"
                     /locus_tag="OQM47_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1480744.1"
                     /translation="PMSSRFFFVLQVNQYTGLGAKVLEACPEVHNLKLAARFTTRTPA
                     NWKNIVDNYLECYHCGPAHLGFSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEG
                     TDAAFHGFWLWPCTMLNVTPIKGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSL
                     IEWYRDVFHPEDLRLVESVQKGLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQV
                     FKD"
BASE COUNT         4930 a         5046 c         5230 g         4903 t
ORIGIN      
        1 ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg acgctgttct
       61 aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg gctatattca
      121 atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg aatgcggatc
      181 cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt tattagcgcg
      241 ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa gataacgcta
      301 tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt acggcggggc
      361 atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc
      421 aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt
      481 gtacgacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc gatgacgaag
      541 aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat acaagcaaat
      601 gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg cacggtggtg
      661 acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat gttgacatgt
      721 gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc cccttccgtc
      781 agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt ggagtaacca
      841 tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat gaagaacagc
      901 agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc aaataccgga
      961 cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat caaaccgact
     1021 ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg ttcttccagc
     1081 gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa gaactccagg
     1141 tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg acgaattgcc
     1201 ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg
     1261 aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt tttctcggtg
     1321 gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt atcgagatcg
     1381 ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg
     1441 atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt aaagttggtg
     1501 aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc acgcatcatg
     1561 ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt cagaccattg
     1621 gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc aacagacagc
     1681 gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga agaaacgtgg
     1741 atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc gtgctggaaa
     1801 gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc cagagggatt
     1861 gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat ggtagaaatg
     1921 atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca gcccagcgcg
     1981 atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag aacgataccc
     2041 accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat tgatcccata
     2101 cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat ctccattgta
     2161 cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac acaatacgtt
     2221 acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg gaaaccttac
     2281 ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata tctttttcat
     2341 caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata ccaccgacgt
     2401 taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt gggttggtaa
     2461 ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga atcattttgg
     2521 caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac
     2581 gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca ttggtttctt
     2641 tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa
     2701 taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct tttggtgctg
     2761 cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct ttcacgcctt
     2821 cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca tcatcacggg
     2881 ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca taatgctctt
     2941 tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta tcaacgagaa
     3001 acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt tcaatcagcg
     3061 tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc tcgcctaaca
     3121 gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct ataacaatag
     3181 caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca gatgtatttt
     3241 tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa acccctgcgt
     3301 ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa
     3361 ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga
     3421 aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt ttaacaacca
     3481 tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc
     3541 cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat tttgtcgtta
     3601 cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg tcatggaatt
     3661 cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg aaattgtgct
     3721 gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc caaaacaacg
     3781 cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc
     3841 gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg atttctcctt
     3901 ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca aagcaacaac
     3961 cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta aaggctacgc
     4021 cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct cgttggatgc
     4081 ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg cggtagtgat
     4141 tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta accagcaccc
     4201 cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc tggtggcaga
     4261 aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct tctcgatcat
     4321 tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt cgactttgcc
     4381 gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa
     4441 tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga
     4501 acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac
     4561 gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa tccgcgagca
     4621 actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat
     4681 cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg atgtggcggc
     4741 gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga tcaacctgct
     4801 gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt ttggtgtggt
     4861 acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat tcccggacgc
     4921 tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag gcggtacaga
     4981 tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg atgatatcgc
     5041 gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat
     5101 tgatgtaggg ccactgcata tcaccatctt tgaagccaat gattatcgtg ttgatctggt
     5161 tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa cgtaacgaca
     5221 taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc gccagccatt
     5281 tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa atatgcacgc
     5341 catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc accagttcaa
     5401 tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta accgcgtcag
     5461 aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt aatgtttcaa
     5521 gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg acgtttttac
     5581 gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt tcggtgattt
     5641 cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga tcttttatca
     5701 gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac tgactactca
     5761 tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc agtggcacaa
     5821 ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag ccctgacgcg
     5881 gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc gcattaagca
     5941 aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt tcgcgggaaa
     6001 agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa cttagcagca
     6061 ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc cccggaccat
     6121 ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca ttgtcttcag
     6181 gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg tccaccgtca
     6241 ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc agtaagtcga
     6301 tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc cattgaatca
     6361 aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt ggttgcggtg
     6421 caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa atgctggagc
     6481 aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt tttgccgcct
     6541 gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga agcaagactt
     6601 tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg tgattggcga
     6661 attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg ctcagggtcg
     6721 aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg cgataggttt
     6781 taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc gacaaacgcc
     6841 tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc gggtataaat
     6901 tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac tttgattgcc
     6961 agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt ttcgtacatc
     7021 gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag agccatccgt
     7081 gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg cacctgaacc
     7141 tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat ttccgccgcc
     7201 acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc agccattgaa
     7261 caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga ggcgttcatt
     7321 ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat gtctgggctg
     7381 accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc gcaggcaccg
     7441 ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac tgccagcgca
     7501 aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc gctggaaact
     7561 aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg cagtacacct
     7621 tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg cgcgaaatac
     7681 tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag accagacagc
     7741 gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa cggatacggc
     7801 acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat aaaaccgccg
     7861 ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc gtggatttgc
     7921 ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt gtcgcgcggg
     7981 aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt tacgtcgcga
     8041 ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc aagacccata
     8101 ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc ataaacgtcc
     8161 acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc gacgaactgt
     8221 ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc caccttaaac
     8281 atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt ccagtaacga
     8341 actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta ttcacatgaa
     8401 ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca agtagtgttt
     8461 tgtagatgta aagataagta tagtcagggg gtcacaccaa tttgcagcgc cagctcacga
     8521 attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag ccatacccga
     8581 tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg taatgcctgg
     8641 gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata cggcagatcg
     8701 ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc gacgggcggc
     8761 agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc agcttcgcgc
     8821 agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg gaatgccact
     8881 tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc cggttgcggt
     8941 cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc ccggttaatt
     9001 tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct acggtattcc
     9061 acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt ttcctgatat
     9121 tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta tatttgtccg
     9181 ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca aaagctcaaa
     9241 tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc gataatttcc
     9301 atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc gcgcagcaga
     9361 tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag atgatgcacg
     9421 gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc tacggcaaca
     9481 cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg atctttcgct
     9541 gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag gcgtggtagc
     9601 gtgcctttaa tcgggcgagt ctggatttca ctattatcac aaagaataaa ccgctctggc
     9661 gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg cgcgcggttg
     9721 gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc atgaaaacgc
     9781 tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac ctggcgaaat
     9841 ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag cgtgaaatct
     9901 tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac atcattatga
     9961 ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc gtagataccc
    10021 actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga ctcaaaacgg
    10081 cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa atcttcgtta
    10141 tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag cgggtcatca
    10201 gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa agtggttaaa
    10261 gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc ggcatagccg
    10321 gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa ttcagcggcg
    10381 tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag tcctgactct
    10441 actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca ggagttaaca
    10501 atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt cgagcggatc
    10561 caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt ggcagaagag
    10621 ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga agacgagtaa
    10681 cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg tgagaaaaag
    10741 gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat aaatttaact
    10801 cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg atttttaatc
    10861 acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc cgtgaaccca
    10921 gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc gggtactccc
    10981 agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga acttggtgtt
    11041 ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac agcaggcata
    11101 ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt aatttgcatc
    11161 taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt caggtgtttt
    11221 aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg gtcagcccgc
    11281 acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc aaagggcgaa
    11341 cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat tgaaattata
    11401 aatataaata atgaattagc agaagcatca acagtaatat cttattctct ggtcgattta
    11461 tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct tcgataaagc
    11521 ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca aacccgcttg
    11581 taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg ctttattcat
    11641 cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc tcgatttatt
    11701 gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg tttgctcaaa
    11761 ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt tgtggattac
    11821 tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt tgtcctcatt
    11881 atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc accacttcaa
    11941 ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg ggtagtgtga
    12001 cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt aagccgcgag
    12061 aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc cagccgctgg
    12121 tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct gcgctgcggg
    12181 cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag ctctacagcc
    12241 gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg ctgctgaaag
    12301 ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg gggggcgatc
    12361 tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat caaatagtcg
    12421 atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg ccgctggtca
    12481 ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat tgctttgtgg
    12541 tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat catctctttc
    12601 tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa gcggacgtca
    12661 tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt ggtcagtcac
    12721 tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac cgcaccgaat
    12781 taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt gcgcaggatt
    12841 ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg ttagctaatc
    12901 cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt tatgatgtgg
    12961 cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc gccacgttgt
    13021 atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc tactggtacg
    13081 aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg gataaattca
    13141 aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg ctgtcggtga
    13201 acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag tcgttgctat
    13261 tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc aatctgccgc
    13321 aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg atcatcgaag
    13381 ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc gatctggccg
    13441 agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc agtaaaggcc
    13501 aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc agtagtttct
    13561 gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac aaattgccgt
    13621 ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt ttgcgcggtg
    13681 gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa cagggggtag
    13741 gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac tatcggctgg
    13801 tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca cgcacccgtg
    13861 acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt gttacggagt
    13921 atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga attctggcta
    13981 tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact gtcaacgctc
    14041 attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg caggatatcc
    14101 tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg cgcggccccg
    14161 gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg cttggcgcgg
    14221 aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg tggcgcaaaa
    14281 acggcgcaac acgcgtgctg tcagccattg atgcgcgaat gggcgaagtt tactgggccg
    14341 aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc gtactcaaac
    14401 ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg gtagggacgg
    14461 gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc gatggcgaag
    14521 tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt gccgagggta
    14581 aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc gcatggaaga
    14641 aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg agtcgcatca
    14701 tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg ctaatgctga
    14761 gcggttgtgt cactgtgccg gacgccatta aaggcagcag taccacgccg caacaagatt
    14821 tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc tttggtggca
    14881 aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct accgtaccgc
    14941 tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt tatgccgatg
    15001 tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta gtcgggccaa
    15061 tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg gtgatgcaag
    15121 taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct cagccgattg
    15181 atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg ggctggtata
    15241 atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt tttaaagaat
    15301 gcagaaacag cggctggtcc gctgtttctg cattcttacg gtaaagataa aaataaatag
    15361 tgacgcgctt cgcaaccttt tcgttgggta attatcaagc tggtatgatg agttaatatt
    15421 atgttaacgg cgtgtatatc atttggggtt gcgatgacga cgaacacgca ttttagaggt
    15481 gaagaattga agaaggtttg gcttaaccgt tatcccgcgg acgttccgac ggagatcaac
    15541 cctgaccgtt atcaatctct ggtagatatg tttgagcagt cggtcgcgcg ctacgccgat
    15601 cagcctgcgt ttgtgaatat gggggaggtg atgaccttcc gcaagctgga agaacgcagt
    15661 cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga agaaaggcga tcgcgttgcg
    15721 ttgatgatgc ctaacttatt gcaatatccg gtggcgctat ttggcatttt gcgtgccggg
    15781 ataatcgtcg taaacgttaa cccgttgtat accccgcgtg agcttgagca tcagcttaac
    15841 gatagcggcg catcggcgat tgttatcgtg tctaactttg cccacactct ggaaaaagtg
    15901 gttgataaaa ccgccgttca gcacgtaatt ctgacccgta tgggcgatca gctatctacg
    15961 gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca agcgcttggt gccgaaatac
    16021 catctgccag atgccatttc atttcgtagc gcactgcata acggctaccg gatgcagtac
    16081 gtcaaacccg aactggtgcc ggaagattta gccttcctgc aatacaccgg cggcaccaca
    16141 ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc tggcgaacct ggaacaggtc
    16201 aacgccacct atgggccgtt gctgcatcca ggtaaagaac tggtagtaac ggcactgccg
    16261 ctgtatcaca tttttgccct gaccattaac tgcctgctgt ttatcgaact gggtgggcag
    16321 aacctgctta tcactaaccc gcgtgatatt ccagggttgg taaaagagtt agcgaaatat
    16381 ccgtttaccg ctatcactgg cgttaacacc ttgttcaatg cgttgctgaa caataaagag
    16441 ttccagcagc tggatttctc cagtctgcat ctttccgcag gcggtgggat gcctgttcag
    16501 caagtggtgg cagaacgctg ggtgaaactg accggacagt atctgctgga aggttatggc
    16561 ctgaccgagt gcgcgccgct ggtgagcgtt aacccgtatg atattgatta tcatagcggt
    16621 agcatcggtt tgccggtgcc atcgacggaa gccaaactgg tggatgacga cgacaacgaa
    16681 gtaccaccgg gtcaaccggg tgagctttgt gtcaaaggac cgcaggtgat gctgggttac
    16741 tggcagcgtc cggatgctac agatgagatc atcaaaaatg gctggttaca caccggcgac
    16801 atcgcggtga tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa agacatgatt
    16861 ttggtttccg gttttaacgt ctatcccaac gagattgaag atgtcgtcat gcagcatcct
    16921 ggcgtacagg aagtcgcggc tgttggcgta ccttccggct ccagtggtga agcggtgaaa
    16981 atcttcgtag tgaaaaaaga tccatcgctt accgaagagt cactggtgac tttttgccgc
    17041 cgtcagctca cgggatacaa agtaccgaag ctggtggagt ttcgtgatga gttaccgaaa
    17101 tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg aagcgcgcgg caaagtggac
    17161 aataaagcct gagcgttaag tcagtcgtca gacgccggtt aatccggcgt tttttttgac
    17221 gcccactaaa gagaaaacaa tttgaattac caaatgatta ccacggacga tgcgctggct
    17281 tctttgtgtg aagccgtccg tgtctttccg gcgatagccc tggatactga atttgttcgt
    17341 acgcgcactt attacccgca gctggggttg attcaacttt tcgatggcga gcatctggcg
    17401 ctaattgatc ctctcgggat caccgactgg tcaccgctga aagcgatcct gcgcgatccg
    17461 tccatcacaa aatttctcca tgcaggcagt gaagatctgg aagtgttcct caatgtcttt
    17521 ggcgaattac cacaaccctt gattgacacg caaatccttg ctgccttctg cggacgcccg
    17581 atgtcatggg gtttcgcttc catggtggaa gagtattccg gcgttacgct ggacaagagt
    17641 gaatcgcgca ccgactggct ggccagaccg ctgaccgaac gtcagtgtga atacgcagcg
    17701 gcggatgtct ggtatctgtt accgatcacc gccaagctta tggtagaaac ggaggcctcc
    17761 ggctggctac ctgcggcgct ggatgaatgc cgcctgatgc aaatgcgtcg tcaggaagtc
    17821 gttgcgccgg aagatgcctg gcgtgatatc accaatgcct ggcagttacg cacacgccaa
    17881 ctggcctgtc tgcaactgtt agccgactgg cgactgcgca aggcgcgaga gcgtgatctg
    17941 gcggtgaact ttgtcgtgcg tgaagagcat ttgtggtcgg tcgcgcgtta tatgccggga
    18001 agtttaggcg aactggacag cctgggttta tccggtagcg aaatccgctt tcacggtaaa
    18061 acgctgctgg cgctggtgga aaaagcgcag acattgccgg aagatgcctt accgcagccg
    18121 atgcttaacc tgatggacat gcctggttat cgtaaagcgt ttaaagcgat taagtcgctg
    18181 attactgacg tgagcgaaac gcataagatc agcgccgaat tgctggcatc gcgtcggcaa
    18241 atcaaccaac tgctgaactg gcactggaaa ctgaaaccgc agaacaattt gccggagctg
    18301 atttccggct ggcgtggtga gctgatggcg gaagcattac acaatttatt gcaggaatat
    18361 ccgcagtaaa atcttccgaa gccggactgg gcgcgctcag cgccacatcc ggctttggca
    18421 aactacaaat ccaacaccag gcgtttaccc ttcgcacgcg aacaacagat caacatactt
    18481 tgctggctgg cacgctcttc atcgctaaaa tactgatccc gatggtcagc ttcaccttcc
    18541 agtattgccg tttcgcaggt tccgcatacc ccttcacgac ataaacattc cactttcgcg
    18601 gctttattat tttcaataac ctgcaaaata gtcatctctt ccggcaccac aaactctttt
    18661 ccggaacggg caagcaccag ggtaaatgca tcgccggttt tgtcttcgat agcaaattgc
    18721 tcaaagtgca gtgtatcggc ggcgatgtcc agacgcgcag cttcacttct taccgcttca
    18781 attagcgcct cggggccaca ggtataaacg tgtgtgccag gttcgatatc cgccaataat
    18841 cgcgctaatt ccagtcgtgt tccggttgat gaatgatgca aatggacttt ctcagcctgc
    18901 ggatgctgga ctagctcagc acgaaatgcg caactttctg gatttcgcga gcagtaatgt
    18961 agctgccagt cgatgtcgct gtgttgcaac tctgccatgt gcgacaggaa aggggtgata
    19021 ccaataccgc ccgcgataaa cagatgcttt ctggctgagg gaattagcgc aaacaggtta
    19081 ttaggcgttg agatcgttaa ccgatcgccc acttttacct gctgatgcaa aaagcgggaa
    19141 ccgccgcgcg agttttcttc cagtcgaacg gcaatctgat aactagaggt gttatgcggt
    19201 gaactcagta gtgaatacgc attgctgtac tggttatcac catcgctcat ctgcactagg
    19261 atgtgacttc ctccggtgaa cgcaggcagc ggtttgccat cggttgccac cagcgtgaag
    19321 cgtttcacct gttcggtaag gggttcaacc tggctcacct gtacttcaaa catttgatag
    19381 tctgacattg ctgtactcca tcaaaaatca gcccagcggc ggaaaatacc gccgccgatg
    19441 tcattagtcc ttaaacacct gcgccagcag attatggaaa tgggcgatac catgttcgga
    19501 aatgccacta ccgctactgt cggccatgat gcgcccctga ccacgatagc cacgcgattt
    19561 cagccctttc tgtacgcttt caaccagacg taaatcttcc ggatggaaca catcgcgata
    19621 ccactcaatc agcgattttt gctcgtcggt taactcttca ttggtgaagt aaatatcgta
    19681 gttttgcagg gtagtttcag aatccaccgg gaattcataa atgaccgtca tcatcccttt
    19741 gatcggggtg acgttcagca tcgtgcacgg ccacagccag aaaccgtgga atgccgcatc
    19801 cgtaccctct tcaaatttaa acgactgttc ggacggtttg gcgaaaccgt attgcagcgt
    19861 ccagttaccg tgcatggtgt gccagtaacg atcaacctgt acggagtcgg agaaacctag
    19921 atgcgccgga ccacagtgat agcactcgag atagttatcg acaatgttct tccagttggc
    19981 aggcgtgcgg gtggtaaagc gggccgctag tttcagattg tggacttccg ggcaggcttc
    20041 cagcactttc gcgcccagac cggtgtattg attcacttga agtacgaaaa aaaaccggga
    20101 ggacattgg
//
LOCUS       DAJVPT010000075        20074 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVPT010000075 DAJVPT010000000
VERSION     DAJVPT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            233..574
                     /gene="raiA"
                     /locus_tag="OQM47_002645"
     CDS             233..574
                     /gene="raiA"
                     /locus_tag="OQM47_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1480745.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    677..802
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            678..725
                     /gene="pheL"
                     /locus_tag="OQM47_002646"
     CDS             678..725
                     /gene="pheL"
                     /locus_tag="OQM47_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCS1480746.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            824..1984
                     /gene="pheA"
                     /locus_tag="OQM47_002647"
     CDS             824..1984
                     /gene="pheA"
                     /locus_tag="OQM47_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1480747.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2027..3148)
                     /gene="tyrA"
                     /locus_tag="OQM47_002648"
     CDS             complement(2027..3148)
                     /gene="tyrA"
                     /locus_tag="OQM47_002648"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1480748.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3159..4229)
                     /gene="aroF"
                     /locus_tag="OQM47_002649"
     CDS             complement(3159..4229)
                     /gene="aroF"
                     /locus_tag="OQM47_002649"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1480749.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4439..4804
                     /locus_tag="OQM47_002650"
     CDS             4439..4804
                     /locus_tag="OQM47_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1480750.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4954..5472
                     /locus_tag="OQM47_002651"
     CDS             4954..5472
                     /locus_tag="OQM47_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1480751.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5462..6687
                     /gene="dgcN"
                     /locus_tag="OQM47_002652"
                     /pseudo
     CDS             5462..6687
                     /gene="dgcN"
                     /locus_tag="OQM47_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6703..7185
                     /locus_tag="OQM47_002653"
     CDS             6703..7185
                     /locus_tag="OQM47_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1480752.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7262..7609)
                     /gene="rplS"
                     /locus_tag="OQM47_002654"
     CDS             complement(7262..7609)
                     /gene="rplS"
                     /locus_tag="OQM47_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1480753.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7651..8418)
                     /gene="trmD"
                     /locus_tag="OQM47_002655"
     CDS             complement(7651..8418)
                     /gene="trmD"
                     /locus_tag="OQM47_002655"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1480754.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8449..8997)
                     /gene="rimM"
                     /locus_tag="OQM47_002656"
     CDS             complement(8449..8997)
                     /gene="rimM"
                     /locus_tag="OQM47_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1480755.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9016..9264)
                     /gene="rpsP"
                     /locus_tag="OQM47_002657"
     CDS             complement(9016..9264)
                     /gene="rpsP"
                     /locus_tag="OQM47_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1480756.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9401..10762)
                     /gene="ffh"
                     /locus_tag="OQM47_002658"
     CDS             complement(9401..10762)
                     /gene="ffh"
                     /locus_tag="OQM47_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1480757.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10929..11720
                     /locus_tag="OQM47_002659"
     CDS             10929..11720
                     /locus_tag="OQM47_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1480758.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11741..13027
                     /locus_tag="OQM47_002660"
     CDS             11741..13027
                     /locus_tag="OQM47_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1480759.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13082..13675)
                     /gene="grpE"
                     /locus_tag="OQM47_002661"
     CDS             complement(13082..13675)
                     /gene="grpE"
                     /locus_tag="OQM47_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1480760.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13798..14676
                     /gene="nadK"
                     /locus_tag="OQM47_002662"
     CDS             13798..14676
                     /gene="nadK"
                     /locus_tag="OQM47_002662"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1480761.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14762..16423
                     /gene="recN"
                     /locus_tag="OQM47_002663"
     CDS             14762..16423
                     /gene="recN"
                     /locus_tag="OQM47_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1480762.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16572..16913
                     /gene="bamE"
                     /locus_tag="OQM47_002664"
     CDS             16572..16913
                     /gene="bamE"
                     /locus_tag="OQM47_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1480763.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16975..17265)
                     /locus_tag="OQM47_002665"
     CDS             complement(16975..17265)
                     /locus_tag="OQM47_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1480764.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17255..17692)
                     /gene="ratA"
                     /locus_tag="OQM47_002666"
     CDS             complement(17255..17692)
                     /gene="ratA"
                     /locus_tag="OQM47_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1480765.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17863..18345
                     /gene="smpB"
                     /locus_tag="OQM47_002667"
     CDS             17863..18345
                     /gene="smpB"
                     /locus_tag="OQM47_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1480766.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18560..18922
                     /gene="ssrA"
                     /locus_tag="OQM47_002668"
     tmRNA           18560..18922
                     /gene="ssrA"
                     /locus_tag="OQM47_002668"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19127..>20074
                     /locus_tag="OQM47_002669"
     CDS             19127..>20074
                     /locus_tag="OQM47_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1480767.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPM"
BASE COUNT         5190 a         5265 c         4686 g         4933 t
ORIGIN      
        1 cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa
       61 atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac
      121 aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca
      181 tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat
      241 gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg
      301 tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa
      361 agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc
      421 cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg
      481 gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga
      541 cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac
      601 gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactaaagt
      661 cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt tttaccttcc
      721 cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa taaggcctcc
      781 caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaaccc
      841 gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cgttactggc
      901 agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
      961 acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg gtaaagcgca
     1021 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     1081 aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact cagcacgcat
     1141 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     1201 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     1261 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     1321 aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     1381 aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca tcaatacggt
     1441 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg
     1501 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     1561 accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     1621 ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt tggcgcgtaa
     1681 agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg cgaccgggca
     1741 acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac
     1801 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct atctggatat
     1861 tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac
     1921 ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg ttgatccaac
     1981 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcgattgt
     2041 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     2101 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     2161 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     2221 acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc ccgaccatcg
     2281 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     2341 cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc tgaataaacg
     2401 ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc cagacctgaa
     2461 tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag accacaactt
     2521 gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt accgggccat
     2581 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     2641 tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg tggattggca
     2701 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     2761 gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac aggcgtccca
     2821 tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt gttttaaatc
     2881 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     2941 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     3001 cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc acttcagcaa
     3061 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     3121 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcagc
     3181 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     3241 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc agaagactga
     3301 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt accatctttg
     3361 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     3421 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     3481 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatcacatgc
     3541 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     3601 aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     3661 gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     3721 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     3781 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcaac
     3841 aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat atggggatcg
     3901 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     3961 agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc atattccaga
     4021 gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg aggatcgcgc
     4081 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     4141 ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc gtcggtaata
     4201 tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct cgtttgcgat
     4261 agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     4321 tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt gcttctgtat
     4381 agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg agaaagaaat
     4441 gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc agattgatcc
     4501 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta tggaagatgc
     4561 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
     4621 tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg attttactta
     4681 tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg aaaatgccag
     4741 ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa tacgattaaa
     4801 ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt tttatttctc
     4861 acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa aaaatatcga
     4921 tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg actgttctta
     4981 cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc tgacgttgcg
     5041 aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc tgcgctatca
     5101 gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct acaagagaat
     5161 gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga ggcgatgatt
     5221 tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat ggaattaacc
     5281 gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga gtgcattatt
     5341 ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt aaacctggat
     5401 gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc acggaagaaa
     5461 aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag cattacgcaa
     5521 catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc tttccgtgac
     5581 ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag cagcaacaat
     5641 gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa ctgaaacact
     5701 ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa gcagcaaaat
     5761 attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag caatttcata
     5821 agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg tgaaaccatt
     5881 ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg gttttcgctc
     5941 gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct cacccgccat
     6001 ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca tgatgtgcgt
     6061 tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt tcaccgcttc
     6121 gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg tttacaggcc
     6181 aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct ggctaaccgc
     6241 gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg aaaaacgtcg
     6301 gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg gggtcatgcg
     6361 acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg cgggctgcga
     6421 cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga tgtacagtcg
     6481 gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct cccgtttgat
     6541 cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat gaccattgag
     6601 cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca ggccaaacac
     6661 cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa gcacctggta
     6721 gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca gggaaagttt
     6781 actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc cggcgactgg
     6841 tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt gctcccggaa
     6901 agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct aacacatgcc
     6961 cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg cttatcattg
     7021 aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc acgcagcaat
     7081 ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac cgcccagggc
     7141 cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac gagcaaacag
     7201 acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct taagcgaaat
     7261 cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc aggtagtaca
     7321 gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact accggagagt
     7381 gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca gtgaatgcag
     7441 agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc agacgttttt
     7501 tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg aaggaaggta
     7561 cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata atttaatctc
     7621 ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt tatgttgctg
     7681 ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag tcagagccag
     7741 gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt tcaaacgcca
     7801 gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca tcccttctaa
     7861 cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg aatcttccgt
     7921 tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt caatcagcgt
     7981 cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt cttcgtcaat
     8041 ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca gaatcaattt
     8101 ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct gtggtgacag
     8161 ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg cgtcccgcaa
     8221 gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt ccacggtacg
     8281 gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca ggccattttt
     8341 aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag gaaacaggct
     8401 aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt aaaaaccagg
     8461 atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa cctgcccatc
     8521 gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg ccttaatgac
     8581 gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat cgtagccttc
     8641 agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct cttcaagctg
     8701 aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg ccgcatcacg
     8761 atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc agctttccag
     8821 ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt caaaaatgct
     8881 ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt aagacgaacc
     8941 catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt tgctcatcat
     9001 gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag caacgcgatc
     9061 agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca ggcgagtgcc
     9121 ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga agcgaccgtt
     9181 gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt tagcgccgtg
     9241 acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa ccggacccca
     9301 tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat ttctggagaa
     9361 aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca ggaaatcccg
     9421 gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc ttcattttct
     9481 tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc tgtacctgca
     9541 tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct ggcttagcgc
     9601 gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact ttatcgtcca
     9661 tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc atcaagctgg
     9721 ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg agatcgaagc
     9781 cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca actttgcttt
     9841 cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac gcgatgcggt
     9901 ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac ttgatcggtt
     9961 tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg tccactttgg
    10021 tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta ttggccgcat
    10081 cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc gacgcatgga
    10141 cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg gtatccacca
    10201 gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca atatcaaccg
    10261 gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc agcgtctcaa
    10321 gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact ttcttcttgt
    10381 gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg gcaccttgca
    10441 ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc tggttctctt
    10501 cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc cccggcgtca
    10561 ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata aactcacgca
    10621 ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc agcgtatctt
    10681 ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg cgcgacaaac
    10741 gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg gccgcaatcg
    10801 cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt gttatgcaac
    10861 ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct caaactgtcg
    10921 acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc agtcttgcgc
    10981 tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct attatttctg
    11041 cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg cccgacggtg
    11101 atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg gtgatctgta
    11161 cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc attgtctatg
    11221 cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac atcacccatc
    11281 tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc tatgccacgc
    11341 taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa ctgaagaaca
    11401 agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt aaaatgttcc
    11461 acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc ctgttctaca
    11521 tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc gtggcgtggt
    11581 ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga cgccgcgtcg
    11641 tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc agccgaattg
    11701 tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca tttctactac
    11761 tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt ccgggtccga
    11821 aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac agggtaatcg
    11881 ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa gcctggtgtt
    11941 aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg tcgggatgcg
    12001 tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg tcgtactggt
    12061 ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc
    12121 gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct ggttgctgaa
    12181 tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg tggttagcgg
    12241 ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac aaatttcccg
    12301 tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg ttgatgacat
    12361 catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga aatctattct
    12421 gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt cgctggacga
    12481 cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga aaaaagagtt
    12541 caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg aaggtacgcc
    12601 gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa
    12661 cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag agattgttgg
    12721 cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc aaaacgacgg
    12781 ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct ttaactggca
    12841 tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac tggaagagat
    12901 ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc tcgacgtaca
    12961 ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc
    13021 ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac gaaaacagaa
    13081 attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga ccattcagcg
    13141 tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca gattccacca
    13201 ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg atcacttcaa
    13261 caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg ccttcaacca
    13321 tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc aggctatcaa
    13381 tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct ttttcaatat
    13441 ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa ataccgtcac
    13501 gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt tcatcgcgcg
    13561 gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg tgctgatcca
    13621 tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta ctcatgaatt
    13681 tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga tcagtttcag
    13741 ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct cggaaaaatg
    13801 aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac tgcactgaca
    13861 acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat cgttgagcaa
    13921 caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc ggagattggg
    13981 caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg cgcggcgcgc
    14041 acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct gggtttcctg
    14101 actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga aggtcactac
    14161 atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg ccaaaaacgc
    14221 atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca tatgatagag
    14281 ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg gctaattatt
    14341 tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat tctgaccccc
    14401 tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc acgaccactg
    14461 gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa
    14521 atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt cctgattcgt
    14581 cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt caatacatta
    14641 agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct ctttactgta
    14701 tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca ggaaaacgac
    14761 tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg agattgattt
    14821 tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta ttgcaataga
    14881 tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc
    14941 tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc tgcgctggct
    15001 ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga tcagcagtga
    15061 tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac tgcgcgaact
    15121 gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca ccaaacctga
    15181 gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc aggaaatgac
    15241 cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc aacagttaag
    15301 tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac ttaacgaatt
    15361 taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc tggcgaacag
    15421 cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg gtgaagacgc
    15481 aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga ttggcatgga
    15541 cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga ttgctgaagc
    15601 cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc gactgtttga
    15661 acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg tcagccctga
    15721 ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg acgatcaggc
    15781 cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg cactggaaac
    15841 cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac agctgatcac
    15901 cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg tcaaatatga
    15961 cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa ccaccaaccc
    16021 aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt cccgcatcgc
    16081 actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga tttttgatga
    16141 agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc tgcgtcaact
    16201 cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca
    16261 tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac atatgcaatc
    16321 tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca gtgaagtcac
    16381 acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt tcctgcttca
    16441 cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt aaagtgatga
    16501 aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc ccgaaaagga
    16561 atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta ttgatgttaa
    16621 ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac caggggaact
    16681 atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa caagttgcgt
    16741 acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg ttctatgtct
    16801 tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg acctttaaca
    16861 gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac taataataaa
    16921 gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg tctgttattt
    16981 attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa tgagagggcg
    17041 ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg gacggctgta
    17101 aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca agccactggc
    17161 gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca ggtattgctt
    17221 ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa acctctttcg
    17281 cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg cgaccaaagg
    17341 cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata cgacacgcct
    17401 cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc ccatccacca
    17461 gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac gttttgctga
    17521 tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac tccagaatcc
    17581 gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc actaactgat
    17641 acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc ataacaattt
    17701 ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa acgctatccc
    17761 ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca ggattgatat
    17821 ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca
    17881 cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga atactttatc
    17941 gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc cctgcgcgca
    18001 ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc atttctgttt
    18061 ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga tcctacccgt
    18121 acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg cgtcaatcga
    18181 gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg caaagtgaaa
    18241 atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa agaacgcgaa
    18301 tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg cactccaatt
    18361 attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat cttattggct
    18421 atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat caggctacat
    18481 gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca taaatctggt
    18541 atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg aaacccaagg
    18601 tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa
    18661 aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc ctccgctctt
    18721 aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc tgcctggggt
    18781 tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg cagctggcaa
    18841 gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc ggacgcgggt
    18901 tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc atccgatgaa
    18961 gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga atttgtcccg
    19021 cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta ggcacttcat
    19081 aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg caagacaaac
    19141 caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg actacgttct
    19201 ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa tctggcagtt
    19261 tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc cttacccgtc
    19321 agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc tagctaaaca
    19381 gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag ccaaaaccaa
    19441 tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg tgacagagga
    19501 ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga ttggcgacgt
    19561 tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg ttcaggccag
    19621 aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca tgatctatgc
    19681 ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag ccttcgaaaa
    19741 gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac tgatgcagac
    19801 aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc aacttcttac
    19861 tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca tagaagcgca
    19921 agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg ttccattgtc
    19981 agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc gagaatttat
    20041 ctttcctagc cgtatcaagc ccaaccagcc tatg
//
LOCUS       DAJVPT010000076        20063 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVPT010000076 DAJVPT010000000
VERSION     DAJVPT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            347..406
                     /gene="ibsE"
                     /locus_tag="OQM47_002670"
     CDS             347..406
                     /gene="ibsE"
                     /locus_tag="OQM47_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1480768.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(526..1959)
                     /gene="hldE"
                     /locus_tag="OQM47_002671"
     CDS             complement(526..1959)
                     /gene="hldE"
                     /locus_tag="OQM47_002671"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1480769.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2007..4847)
                     /gene="glnE"
                     /locus_tag="OQM47_002672"
     CDS             complement(2007..4847)
                     /gene="glnE"
                     /locus_tag="OQM47_002672"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1480770.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4870..6171)
                     /gene="ygiF"
                     /locus_tag="OQM47_002673"
     CDS             complement(4870..6171)
                     /gene="ygiF"
                     /locus_tag="OQM47_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1480771.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6413..7033
                     /locus_tag="OQM47_002674"
     CDS             6413..7033
                     /locus_tag="OQM47_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1480772.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7097..8335
                     /gene="cca"
                     /locus_tag="OQM47_002675"
     CDS             7097..8335
                     /gene="cca"
                     /locus_tag="OQM47_002675"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1480773.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8498..9319)
                     /gene="bacA"
                     /locus_tag="OQM47_002676"
     CDS             complement(8498..9319)
                     /gene="bacA"
                     /locus_tag="OQM47_002676"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1480774.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9410..9778)
                     /gene="folB"
                     /locus_tag="OQM47_002677"
     CDS             complement(9410..9778)
                     /gene="folB"
                     /locus_tag="OQM47_002677"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1480775.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9883..10500
                     /gene="plsY"
                     /locus_tag="OQM47_002678"
     CDS             9883..10500
                     /gene="plsY"
                     /locus_tag="OQM47_002678"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1480776.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10513..11444)
                     /gene="ttdR"
                     /locus_tag="OQM47_002679"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10513..11444)
                     /gene="ttdR"
                     /locus_tag="OQM47_002679"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11651..12494
                     /gene="ttdA"
                     /locus_tag="OQM47_002680"
                     /pseudo
     CDS             11651..12494
                     /gene="ttdA"
                     /locus_tag="OQM47_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12491..13096
                     /gene="ttdB"
                     /locus_tag="OQM47_002681"
     CDS             12491..13096
                     /gene="ttdB"
                     /locus_tag="OQM47_002681"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1480777.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13144..14607
                     /gene="ttdT"
                     /locus_tag="OQM47_002682"
     CDS             13144..14607
                     /gene="ttdT"
                     /locus_tag="OQM47_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1480778.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14650..15663)
                     /gene="tsaD"
                     /locus_tag="OQM47_002683"
     CDS             complement(14650..15663)
                     /gene="tsaD"
                     /locus_tag="OQM47_002683"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1480779.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15901..16116
                     /gene="rpsU"
                     /locus_tag="OQM47_002684"
     CDS             15901..16116
                     /gene="rpsU"
                     /locus_tag="OQM47_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1480780.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16227..17972
                     /gene="dnaG"
                     /locus_tag="OQM47_002685"
     CDS             16227..17972
                     /gene="dnaG"
                     /locus_tag="OQM47_002685"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1480781.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18167..20008
                     /gene="rpoD"
                     /locus_tag="OQM47_002686"
     CDS             18167..20008
                     /gene="rpoD"
                     /locus_tag="OQM47_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1480782.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4780 a         5584 c         5135 g         4564 t
ORIGIN      
        1 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
       61 tcacccttgt gcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac
      121 acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca
      181 aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg
      241 gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa
      301 cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt
      361 catcatactg gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg
      421 ggggagaaat cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca
      481 ataggcctgc ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc
      541 ctgttggatc ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag
      601 cacttcgccg ccgttggccc agacttcttt actcccggcg atctcttctg gtttatagtc
      661 gccgcccttc accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc
      721 ttcaaacgac accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc
      781 gagcgggtta acggggcgag aatccccttt cagccgtttg gtggaggcat cgctgttcac
      841 agcgacaatc aagcggtcac ccagtttgcg ggcatttgcc agatagaaga cgtgtccggc
      901 gtgcaggatg tcaaagacgc cattggtcat caccactttt tcaccgcgtt tacgcgctgc
      961 cgctacggcc agcttcagtt cctcttcggt catcacgcca aagccggtat ctgcgcgccc
     1021 acgcactgca ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac
     1081 cacgccagcc gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag
     1141 cgttgccgcc aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg
     1201 cgcctgggtt ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc
     1261 ggaacgggtc actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc
     1321 aacaatctct tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg
     1381 tgtcagcagc gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac
     1441 cggaacgccc gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt
     1501 ggcgtagtca gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg
     1561 cagcggctgc ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg
     1621 gttgcgggaa agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc
     1681 gcatttgacg ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc
     1741 cgtcaacccg accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc
     1801 cgcgccgccc ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg
     1861 cgagatacga ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat
     1921 cactcctgca cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca
     1981 aaatttgcgc gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc
     2041 cgcaccagat cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt
     2101 aatgccagat gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct
     2161 tcctgctctt ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac
     2221 cagcgcgtta acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat
     2281 tcgatatcgg taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg
     2341 ccgagatgag cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct
     2401 tctcgcggca gtgtcataat ctcacgacgc accgcgtcaa agtgcgcagt gagctgcgga
     2461 tcgccgtaca ctacacgcgc acgcaccagc gcctgatgtt cccacgtcca ggcctcgttt
     2521 ttctgataat cggcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga
     2581 cgcagtcgag catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc
     2641 ataatgcgtt gcgccagacg cagataaaac tgccgcccgt cgatttcccg ctcgccgtca
     2701 gtcatcgcat ccattgggca atcatggagg aagataaggt caagatcgga actgtagcct
     2761 aactcccagc cgcccagctt gccgtagccg accaccgcaa aaccgcgccc ttcacgttcg
     2821 ttgaggtgat ttggcttacc gtagcgggcc accatttgac cccacgcctg ctgaacgacg
     2881 gcatctatca tagcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc
     2941 gtaccggcga tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc
     3001 gcctcaagct gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca
     3061 tcacggtagg catcggtcgc cgtcggctga taaagggtat ttggatcgag caattcatcc
     3121 agcaataatg gataacgcgc tagctggctg gcaatcatcg gcgatgcggc acacagagaa
     3181 atcaaatgtt taagcgccgc agggaattca ctgagcaatt ctaaataggt tgtgcgggta
     3241 acaatcccca ccagcaaggc ggtaatgcgc gacagcgtga cagcagcgtc ttcacgcgca
     3301 cagacatcac ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg
     3361 atagtacgct tatccagctc tttgcggaaa tcagcaatca tcgttaggac ctgtttgcga
     3421 tcatcctcgc taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc
     3481 cacagctcac gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca
     3541 atcaattcat taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt
     3601 tgcggccagt cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa
     3661 ggaagcgttt gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg
     3721 aacagatatg ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca
     3781 atggcgctga gcgttggcag taaagagcgc gactgcagca acggttcgcg cccaccgcga
     3841 atgagctgga acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg
     3901 atattgtcgg tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc
     3961 agcgactgaa tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca
     4021 cgcaactcgt tagcatagac gccttcgcta tcgcccataa tccgcgcttt gaccatcgcg
     4081 taacgctccc agtcgcgtcc ctgctcctgg taataatcct ccaacgcggc aaagctcagc
     4141 accagcgggc cactttcgcc aaacggacgc agtcgcatat ccacgcgata gacgaagcca
     4201 tcctgcgttg gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc
     4261 gcgttatcca gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc
     4321 agatcgatat cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaagatc
     4381 agcagcggtt gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaggcg
     4441 tcatacagcc agtcacgcgc cgcaacaatc agcgtctccg ccagataact gagctgctgc
     4501 aatatgctct cttcagtaac cagtgccagc gtttgcgccc atgcgatgcg caccataatg
     4561 cgccgccgga atagccgcag ctcgcgcatt aacccggctt cgtcgctcac attactgagc
     4621 gcctcctgca accacacgct gtaatgttgc cattcgtcag cctgtggcgg ttggctttcc
     4681 agttccgtca gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta
     4741 agtactgact tcgcctgtgc actaagtgat tcctcggcta aaagctctgg cagccgctca
     4801 acaacggtct gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc
     4861 cttgcctgat taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt
     4921 acggaaatgt tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca
     4981 gttctccagc caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga
     5041 gtcaatatca cgcatcaggc gtggcaactg gtcacggtag cgatcgccta acggctggca
     5101 gaaaacgctt ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa
     5161 ggagtcgcta attttgccct gcgctttggc atctaaaaac ggctgccatg ctttgcttac
     5221 cagccattcg gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccgtcac
     5281 ggcagaaacc gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt
     5341 actcgcttta cgcggcacaa tgccaccgaa cagcatcagt gtatgacgga ctaggctaat
     5401 ggctgccagc acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata
     5461 ctgccattgc gctaacacca gctcgagcgc cgcttccagc ccctgttcca catcggcttt
     5521 tgccgcaaca tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag
     5581 atgatagcca cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag
     5641 ttggttcgcc agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca gttccagctc
     5701 gcagataggc tcagcaaatt cacccgcttt cacttccccc tggtcgagag cgatttcaat
     5761 tcggctgcca tcgatctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcga
     5821 ctgcacgcgg gaagcgagat cggcaggcaa ttcgccgttc ggccagattt ccgtcggtaa
     5881 ctgcgccagg tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa
     5941 gccgcctgtc actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg
     6001 aatacgtaag cccatatcgt gcctacgcag ccagttatcc ggcgtttcgt agtaaatatt
     6061 cagcaactgc acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc
     6121 ctcaacggca ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac
     6181 ttatcggtta tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca
     6241 gtagatagta ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt
     6301 gaagcagtgg caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg
     6361 acactgatgc cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa
     6421 attacgcctg atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga
     6481 agaaacgcgc tatgtttctg acgaactgaa tacctgggtc cgtagcggtc cgggagatca
     6541 ttatcgcctc gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc
     6601 caacaccaat tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa
     6661 acaacttagc actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa
     6721 aaccctgacc gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat
     6781 gcagcaaaaa gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag aaaatcagaa
     6841 actgaaaaac gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga
     6901 tgacaaacag cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct
     6961 tggcttgctg ctcggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg
     7021 ctggatgaac taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg
     7081 acgaagggat gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt
     7141 aggactatcg gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct
     7201 cgacgcgggc taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca
     7261 tgaagagtat gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac
     7321 ttgctatacc gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat
     7381 taatgcgctg gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga
     7441 tctgcaaaat cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt
     7501 actgcgcgtg gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga
     7561 aactctgacg ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga
     7621 acgggtatgg aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca
     7681 ggtactgcgc gattgcggcg cacttcgcgt tttattcccg gaaattgacg cactgtttgg
     7741 cgttccggca cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac
     7801 gctctcaatg gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca
     7861 cgatctcggt aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc
     7921 ggcgggtgtt aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg
     7981 cgatttagcc agactggtgg ctgagtttca cgatctcatc cgcaccttcc caatgctgaa
     8041 cccaaaaacc atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt
     8101 cgagcaactg gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc
     8161 ggactacccg caaggccgtt ggttgcgcga aggctgggaa gtggcgcagt cagtgccgac
     8221 aaaagcggtc gttgaagcgg gatttaaagg cgtggagatt cgcgaggagc tgacccgacg
     8281 gcggattgcg gcggtagcca gctggaagga acaacgttgc ccaaagcctg actgagtata
     8341 aaactaaacg ttagcacgaa ccgtcccctc gccccttcgg ggagagggtt agggtgaggg
     8401 gaacagacca gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga
     8461 gggagaaaac ccggcctgct cccgaacctt ttctcaatca aaagaacacg acatacaccg
     8521 cagccgccac aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt
     8581 gcaggaaggt tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa
     8641 acatcgggat atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg
     8701 cgcccatcat catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca
     8761 cgcccatcag catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg
     8821 ccagacactg gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg
     8881 gcgcacgcgg ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga
     8941 ccagcgcata catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaataata
     9001 gccccagtac caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt
     9061 tgctttcacc ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac
     9121 gccgccagaa catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg
     9181 ttttcgccgt gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc
     9241 tggataccgg cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca
     9301 gcgagtgcat atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgaa
     9361 ctactgcagt atgaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt
     9421 ctttcagatt attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc
     9481 ctggcttgct gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg
     9541 ccacctcttc agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca
     9601 ccgtttctgc aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt
     9661 tacggttatc ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc
     9721 agtcgtaaac accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca
     9781 cgtcctgcct gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca
     9841 cagattcatc gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgcct
     9901 ggaatgatcc tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc
     9961 ttgtgtgggc tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg
    10021 ttacgtatcg gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga
    10081 atgttgcctg tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt
    10141 gccatcgccg cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa
    10201 ggcgttgcta ccgcttttgg agcaatcgca ccaattagct gggatctcac cggagtaatg
    10261 gcgggaacct ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc
    10321 agtgcactga ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt
    10381 tcgatgctct cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt
    10441 cgtcaggaga caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga
    10501 tttctggtgg atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca
    10561 ccatgccgcc agaaattccg cgcagacacg aatttcatgc tgcgatagag cagctcccgg
    10621 taaacagccc agatattggc gctttgtgca tactccggca atacttgcac cagtttgcca
    10681 ctctccagaa acggcaacac atcccactcg gaacgcagca taatcccttt gccctccagc
    10741 gcccattgca acactatctc gccgctattg gaggaaagat gcccgctaac cttcaccgat
    10801 tttttctcct gaccgttccc caactcccat attccgtggg tcatatcgcg ctctttggtc
    10861 accagacagt catgacgact taattcctgt aatgattgtg gctgcggata tttttgcaga
    10921 tactcaggtg ctgcgcataa tattctttta tttttcgtta acagatgcgc aatataataa
    10981 tcaggaattt cgtcattaat acgaatatcc agatcaatat tatcctgcac taaatcaatt
    11041 tgccgatcga acaattcaaa atgcacctgt aactcaggat aattgcgcat aagttcggta
    11101 atggctggcg caatatggct gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc
    11161 tccgggcgcg ttttgatttg cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt
    11221 tccagcgcat gttcgtaaca acgttggcca ctttcagtca gcgccacgcc gcgagcggag
    11281 cggttcagca gcgttgttgc cagcgtattt tcaaggattt ggatccgctt ggtgacaaat
    11341 gcaggcgttt gcccaagcgt cgccgctgca gcactaaaac tcccgctgtg gacgatttct
    11401 accagtacct gcaagtcttt ggctaaaggc cagctattca gcatcgttaa ttatccgcag
    11461 ttgtgataag cgcagtgtat ttcgcaaaac attgccattc cctgctctgg cgcaatattc
    11521 attcttattt tcgcgttgat tcacgaactg ttaattacat attaacggtg gcaaaaaata
    11581 taaaaccaca tttttagtgt tagtttgtgg cggtgaattt ttccagacaa atacaaaaac
    11641 tggagttgcc atgatgagcg aaagtaataa gcaacaggca gtgaataagt tgacagagat
    11701 tgtcgctaac tttaccgcca tgatttctac ccgaatgcct gatgacgtgg tggataaact
    11761 aaaacagcta aaggatgccg aaacgtcgtc gatggggaaa attatctacc atacgatgtt
    11821 cgacaacatg caaaaagcga ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat
    11881 tatgttcttc gttaaagtcg gttcccgctt cccactgctt ggcgagctgc aaagcatact
    11941 caaacaagcc gtggaagagg caaccgtcaa agcgccacta cgtcacaatg cggtagaaat
    12001 ttttgacgaa gtaaacaccg gcaaaaatac cggtagcggc gtaccgtggg tcacctggga
    12061 catcatcccc gacaatgacg atgcggaaat cgaagtttac atggcaggcg gcggctgcac
    12121 gctacctggc cgctcgaaag tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt
    12181 cgtcttcgaa aatatctcca ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt
    12241 gggcatcgcc acctcggtgg aaaccgccgc cgtactctcg cgtaaagcca ttttgcgccc
    12301 gattggcagc cgccatccca atccaaaagc ggcagaactg gagctacgcc tggaagaagg
    12361 actcaaccgt ctggggattg gtccacaagg gctgaccggc aacagttcag tgatgggcgc
    12421 atcgtcgcgg cacgctgctg gttcatgccg atctcacctt tgaaaatctg tctcacaccc
    12481 ggagcgcgtt atgaaaaaga tcctgacaac cccgatcaaa gctgaagatc tgcaagatat
    12541 tcgcgtcggc gatgtgatct acctgaccgg tacgctggtg acctgccgcg acgtttgtca
    12601 ccgccgtttg atcgaactga aacgtccgat cccttacgat ctcaacggca aagcgatttt
    12661 ccacgctggc cccatcgtgc gcaaaaacgg cgacaaatgg gagatggtct ccgtcggccc
    12721 gacaaccagt atgcgtatgg aaagttttga acgtgaattt attgagcaga ccggcgtaaa
    12781 actggtggtt ggcaaaggtg gtatggggcc gctgaccgaa gaaggctgcc agaaattcaa
    12841 ggcgttacat gtgattttcc cagcaggctg cgcggtgctg gcggcaaccc aggtagaaga
    12901 gattgaagaa gtgcactgga cagaactcgg gatgccggag tcactgtggg tctgccgggt
    12961 caaagagttc ggcccgctga ttgtctctat tgatacccac ggcaacaacc tgatagccga
    13021 aaacaaaaag ctgttcgccg aacgccgcga tcccatcgtg gaagagatct gcgagcacgt
    13081 ccattacatc aaataaccct cccggagagg ctcacccctc tcctttttcg caggcataac
    13141 acgatgaaac cttccactga atggtggcga tatcttgcgc cgctggcggt catcgccatt
    13201 attgctctac ttccggttcc cgcagggctg gagaaccata cctggctcta ctttgccgtt
    13261 tttactggcg tgatcgttgg actgatcctc gaacccgtgc cgggtgccgt ggtggcgatg
    13321 gtgggtatct ccatcatcgc catactctct ccctggctgc tgttcagccc cgaacagctc
    13381 gcccagccag gctttaagtt caccgccaaa tccctctcct gggcagtttc tggtttttct
    13441 aattcggtta tctggctcat tttcgccgcc tttatgtttg gcacaggcta tgaaaaaacc
    13501 gggcttggac gccgcattgc gctgattctg gtgaaaaaga tggggcatcg cacgctgttt
    13561 ctcggctatg cggtgatgtt ctccgagctg atcctggcac ctgtaacacc gtccaactcg
    13621 gcgcgtggtg cgggtattat ctatcccatc atccgtaacc tgccaccgct ctatcaatca
    13681 caaccaaacg acagcagttc gcgcagcatt ggctcgtaca tcatgtggat ggggattgtt
    13741 gccgactgcg tgaccagcgc cattttcctg acggcgatgg cacctaactt gctgttaatt
    13801 ggactgatga aaagcgcatc tcacgccacg ctgagttggg gcgactggtt cctcgggatg
    13861 ttgccgctca gcattttact ggttctgctg gttccctggc tggcttacgt gctgtacccg
    13921 ccggtactga agtctggtga tcaggtgccg cgctgggcag agacggaact gcaggcaatg
    13981 ggcccgctct gttcgcgtga aaaacggatg ctggggctga tggtaggcgc gctggtgctg
    14041 tggattttcg gcggtgatta tatcgatgcc gcgatggtcg gttacagcgt ggtggcgctg
    14101 atgctgcttc tgcgcattat cagttgggac gacattgtca gtaataaagc ggcatggaac
    14161 gttttcttct ggctggcctc gcttatcacc ctcgcgaccg gactcaacaa taccggtttt
    14221 attagctggt ttggcaaact gttagcaggc agcttaagtg gttattcgcc gacgatggtg
    14281 atggtgacgt tgattgtggt gttttatcta ctgcgctact ttttcgccag cgccacggcg
    14341 tatacctgcg ccctcgcacc gatgatgatc gccgccgcgc tggcgatgcc ggaaatcccg
    14401 ctgccggtgt tctgcctgat ggttggcgcg gcaattggtc tggggagcat tcttacacca
    14461 tacgccaccg gacccagtcc gatttactac ggtagtggtt atctgccaac ggcggattac
    14521 tggcgactgg gggcgatttt tgggctgata ttcctcgtgt tgctggtgat taccggctta
    14581 ctgtggatgc ccgtggtgtt gctttaagtg aggacggtgc cggaaattgc tctccggcac
    14641 ccaatagttt tacgcagccg gtaactccgc cagcggccag cgcggacgca cgctaacgcc
    14701 gagatccgcc gtcgcgcctg ctttaaaccg caccattccg gcataggcga tcatcgcgcc
    14761 gttatcagta caaaattccg gacgcgcgta gaacacttcg ccgcggcgtt ttttcatcat
    14821 ttcagccagc ttcgcccgta acgtgcggtt agcactcacg ccgcccgcca tgaccagtcg
    14881 cttaaagccc gtctgatcca acgctcgctt acacttaatc atcaacgtat cgaccaccgc
    14941 atcttcaaag gcgcgggcga tatcagcacg cgtctggtcg tcggtgccgt tgtcacgaat
    15001 cgtgttcgcc gcaaaggttt tcagaccaga aaagctgaaa tccagccccg gacggtcggt
    15061 catcggacgc gggaaaacaa agcgcccggc agtaccctga gccgccattt tcgacagtaa
    15121 cggtccgcca ggataatcca gccccagcag cttcgcggtt ttatcaaacg cttcaccggc
    15181 ggcatcatcg atagactcgc cgagcagctc gtactgacca atgccagtca cgctgattaa
    15241 ctgcgtatga ccgccggaca ccagcagcgc gacaaacgga aattccggcg ggttatcttc
    15301 cagcatcggc gctaacagat gcccttccat atggtgtacc gggattgccg gaacgttcca
    15361 ggcaaacgcc agagaacgcc ccacggttgc gccaaccagc agtgcgccga ctaatccagg
    15421 gcctgcggta taggccacag catcaatgtc ttttgccgtt aaaccagact cctttagcgc
    15481 cgcctggatc aacggtacgg ttttacgcac atgatcgcgg gaggccagtt caggcacgac
    15541 gccgccgtag tcagcgtgca atttcacctg actatacaat tggttggcta acaaaccttt
    15601 ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa gtttcaatac ccagtacacg
    15661 catgacttgt tttacctcgc tttattaccg cgcagtgtag gaccaatgcg ggttgatgta
    15721 aaactttgtt cgcccctgga gaaagcctcg tgtatactcc tcacccttat aaaagtccct
    15781 ttcaaaaaag gccgcggtgc tttacaaagc agcagcaatt gcagtaaaat tccgcaccat
    15841 tttgaaataa gctggcgttg atgccagcgg caaaccgaat taatcaaagg tgagaggcac
    15901 atgccggtaa ttaaagtacg tgaaaacgag ccgttcgacg tagctctgcg tcgcttcaag
    15961 cgttcctgcg aaaaagcagg tgttctggcg gaagttcgtc gtcgtgagtt ctatgaaaaa
    16021 ccgactaccg aacgtaagcg cgctaaagct tctgcagtga aacgtcacgc gaagaaactg
    16081 gctcgcgaaa acgcacgccg cactcgtctg tactaattcc ccgagagcgt tgctctccga
    16141 tcagaccgag ttgtagttgt aaggccgtgc ttccgaaagg aatgcgcggc ttattttcgt
    16201 ttatgaattg ctaaaaatcg gggcctatgg ctggacgaat cccacgcgta ttcattaatg
    16261 atctgctggc acgcactgac atcgtcgatc tgatcgatgc ccgtgtgaag ctgaaaaagc
    16321 agggcaagaa tttccacgcg tgttgtccat tccacaacga gaaaaccccg tcattcaccg
    16381 ttaacggtga gaaacagttt taccactgct ttggatgtgg cgcgcacggc aacgcgatcg
    16441 acttcctgat gaactacgac aagctcgagt tcgtcgaaac ggtcgaagag ctggcagcaa
    16501 tgcacaatct tgaagtgcca tttgaagcag gcagcggccc cagccagatc gagcgccatc
    16561 agaggcaaac gctttatcag ttgatggacg gtctgaatac gttttaccaa caatctttac
    16621 aacaacctgt tgccacgtct gcgcgccagt atctggaaaa acgcggatta agccacgagg
    16681 ttatcgctcg ctttgcgatt ggttttgcgc cccccggctg ggacaacgtc ctgaagcggt
    16741 ttggcggcaa tccagaaaat cgccagtcat tgattgatgc ggggatgttg gtcactaacg
    16801 atcagggacg cagttacgat cgtttccgcg aacgggtgat gttccccatt cgcgataaac
    16861 gcggtcgggt gattggtttt ggcgggcgcg tgctgggcaa cgataccccc aaatacctga
    16921 actcgccgga aacagacatt ttccataaag gccgccagct ttacggtctt tatgaagcgc
    16981 agcaggataa cgctgaaccc aatcgtctgc ttgtggtcga aggctatatg gacgtggtgg
    17041 cgctggcgca atacggcatt aattacgccg ttgcgtcgtt aggtacgtca accaccgccg
    17101 atcacataca actgttgttc cgcgcgacca acaatgtcat ttgctgttat gacggcgacc
    17161 gtgcaggccg cgatgccgca tggcgagcgc tggaaacggc gctgccttac atgacagacg
    17221 gccgtcagct acgctttatg tttttgccgg atggcgaaga ccctgacacg ctggtacgaa
    17281 aagaaggtaa agaagcgttt gaagcgcgga tggagcaggc gatgccactc tccgcatttc
    17341 tgtttaacag tctgatgccg caagttgatc tgagtacccc tgacgggcgc gcacgtttga
    17401 gtacgctggc actgccattg atatcgcaag tgccgggcga aacgctgcga atatatcttc
    17461 gtcaggaatt aggcaacaaa ttaggcatac ttgatgacag ccagcttgaa cgattaatgc
    17521 caaaagcggc agagagcggc gtttctcgcc ctgttccgca gctaaaacgc acgaccatgc
    17581 gtatacttat agggttgctg gtgcaaaatc cagaattagc gacgttggtc ccgccgcttg
    17641 agaatctgga tgaaaataag ctccctggac ttggcttatt cagagaactg gtcaacactt
    17701 gtctctccca gccaggtctg accaccgggc aacttttaga gcactatcgt ggtacaaata
    17761 atgctgccac ccttgaaaaa ctgtcgatgt gggacgatat agcagataag aatattgctg
    17821 agcaaacctt caccgactca ctcaaccata tgtttgattc gctgcttgaa ctgcgccagg
    17881 aagagttaat cgctcgtgag cgcacgcatg gtttaagcaa cgaagaacgc ctggagctct
    17941 ggacattaaa ccaggagctg gcgaaaaagt gatttaacgg cttaagtgcc gaagagcgat
    18001 cgggaagccc ccgacagccg cactgagagg cagcggcaaa tatataagta cgccctcgta
    18061 attatcgttg gcggtaaaca accgttggat ttcagcgtta acggctgaag gacatcgggt
    18121 caatcgccca acaccaacct catgaaataa gtgtggatac cgtcttatgg agcaaaaccc
    18181 gcagtcacag ctgaaacttc ttgtcacccg tggtaaggag caaggctatc tgacctatgc
    18241 cgaggtcaat gaccatctgc cggaagatat cgtcgattca gatcagatcg aagacatcat
    18301 ccaaatgatc aacgacatgg gcattcaggt gatggaagaa gcaccggatg ccgatgatct
    18361 gatgctggct gaaaacaccg cggacgaaga tgctgccgaa gccgccgcgc aggtgctttc
    18421 cagcgtggaa tctgaaatcg ggcgcacgac tgacccggta cgcatgtaca tgcgtgaaat
    18481 gggcaccgtt gaactgttga cccgcgaagg cgaaattgac atcgctaagc gtattgaaga
    18541 cgggatcaac caggttcaat gctccgttgc tgaatatccg gaagcgatca cctatctgct
    18601 ggaacagtac gatcgtgttg aagcagaaga agcgcgtctg tccgatctga tcaccggctt
    18661 tgttgacccg aacgcagaag aagatctggc acctaccgcc actcacgtcg gttctgagct
    18721 ttcccaggaa gatctggacg atgacgaaga tgaagacgaa gaagatggcg atgacgacag
    18781 cgccgatgat gacaacagca tcgacccgga actggctcgc gaaaaatttg cggaactacg
    18841 cgctcagtac gttgtaacgc gtgacaccat caaagcgaaa ggtcgcagtc acgctgccgc
    18901 tcaggaagag atcctgaaac tgtctgaagt attcaaacag ttccgcctgg tgccgaagca
    18961 gtttgactac ctggtcaaca gcatgcgcgt catgatggac cgcgttcgta cgcaagaacg
    19021 tctgatcatg aagctctgcg ttgagcagtg caaaatgccg aagaaaaact tcattaccct
    19081 gtttaccggc aacgaaacca gcgatacctg gttcaacgcg gcaattgcga tgaacaagcc
    19141 gtggtcggaa aaactgcacg atgtctctga agaagtgcac cgcgccctgc aaaaactgca
    19201 gcagattgaa gaagaaaccg gcctgaccat cgagcaggtt aaagatatca accgtcgtat
    19261 gtccatcggt gaagcgaaag cccgccgtgc gaagaaagag atggttgaag cgaacttacg
    19321 tctggttatt tctatcgcca agaaatacac caaccgtggc ttgcagttcc ttgacctgat
    19381 tcaggaaggt aacatcggcc tgatgaaagc ggttgataaa ttcgaatacc gccgtggtta
    19441 caagttctcc acctacgcaa cctggtggat ccgtcaggcg atcacccgtt ctatcgcgga
    19501 tcaggcgcgc accatccgta ttccggtgca tatgattgag actatcaaca aactcaaccg
    19561 tatttctcgc cagatgctgc aagagatggg ccgtgaaccg acgccggaag aactggctga
    19621 acgtatgctg atgccggaag acaagatccg caaagtgctg aagatcgcca aagagccaat
    19681 ctccatggaa acgccgatcg gtgatgatga agattcgcat ctgggggatt tcatcgagga
    19741 taccaccctc gagctgccgc tggattctgc gaccaccgaa agcctgcgtg cggcaacgca
    19801 cgacgtgctg gctggcctga ccgcgcgtga agcaaaagtt ctgcgtatgc gtttcggtat
    19861 cgatatgaac accgaccaca cgctggaaga agtgggtaaa cagttcgacg ttacccgcga
    19921 acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg cgtcacccga gccgttctga
    19981 agtgttgcgt agcttcctgg acgattaatc ggtaggccgg atcaggcgtt acgccgcacc
    20041 cggcactagt ctctctgcac aaa
//
LOCUS       DAJVPT010000077        19966 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVPT010000077 DAJVPT010000000
VERSION     DAJVPT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..496)
                     /gene="aroD"
                     /locus_tag="OQM47_002687"
     CDS             complement(<1..496)
                     /gene="aroD"
                     /locus_tag="OQM47_002687"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1480783.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFD"
     gene            complement(527..1393)
                     /gene="ydiB"
                     /locus_tag="OQM47_002688"
     CDS             complement(527..1393)
                     /gene="ydiB"
                     /locus_tag="OQM47_002688"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1480784.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1405..2676)
                     /locus_tag="OQM47_002689"
     CDS             complement(1405..2676)
                     /locus_tag="OQM47_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480785.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(2859..4064)
                     /locus_tag="OQM47_002690"
     CDS             complement(2859..4064)
                     /locus_tag="OQM47_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480786.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4164..4520)
                     /locus_tag="OQM47_002691"
     CDS             complement(4164..4520)
                     /locus_tag="OQM47_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS1480787.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4656..4763)
                     /gene="rprA"
                     /locus_tag="OQM47_002692"
     ncRNA           complement(4656..4763)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQM47_002692"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(4949..6061)
                     /gene="ydiK"
                     /locus_tag="OQM47_002693"
     CDS             complement(4949..6061)
                     /gene="ydiK"
                     /locus_tag="OQM47_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1480788.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6450..9506
                     /locus_tag="OQM47_002694"
     CDS             6450..9506
                     /locus_tag="OQM47_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1480789.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9503..9913
                     /gene="menI"
                     /locus_tag="OQM47_002695"
     CDS             9503..9913
                     /gene="menI"
                     /locus_tag="OQM47_002695"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1480790.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10013..10201
                     /locus_tag="OQM47_002696"
     CDS             10013..10201
                     /locus_tag="OQM47_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1480791.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10749..11117
                     /gene="sufA"
                     /locus_tag="OQM47_002697"
     CDS             10749..11117
                     /gene="sufA"
                     /locus_tag="OQM47_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1480792.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11126..12613
                     /gene="sufB"
                     /locus_tag="OQM47_002698"
     CDS             11126..12613
                     /gene="sufB"
                     /locus_tag="OQM47_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1480793.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12623..13369
                     /gene="sufC"
                     /locus_tag="OQM47_002699"
     CDS             12623..13369
                     /gene="sufC"
                     /locus_tag="OQM47_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1480794.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13344..14615
                     /gene="sufD"
                     /locus_tag="OQM47_002700"
     CDS             13344..14615
                     /gene="sufD"
                     /locus_tag="OQM47_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1480795.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14612..15832
                     /gene="sufS"
                     /locus_tag="OQM47_002701"
     CDS             14612..15832
                     /gene="sufS"
                     /locus_tag="OQM47_002701"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1480796.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15845..16261
                     /gene="sufE"
                     /locus_tag="OQM47_002702"
     CDS             15845..16261
                     /gene="sufE"
                     /locus_tag="OQM47_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1480797.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16410..17414
                     /gene="ldtE"
                     /locus_tag="OQM47_002703"
     CDS             16410..17414
                     /gene="ldtE"
                     /locus_tag="OQM47_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1480798.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17477..17713)
                     /gene="lpp"
                     /locus_tag="OQM47_002704"
     CDS             complement(17477..17713)
                     /gene="lpp"
                     /locus_tag="OQM47_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1480799.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18024..19436)
                     /gene="pykF"
                     /locus_tag="OQM47_002705"
     CDS             complement(18024..19436)
                     /gene="pykF"
                     /locus_tag="OQM47_002705"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1480800.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5235 a         4782 c         5102 g         4847 t
ORIGIN      
        1 cgtcgaagga ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat
       61 ggaagtcatg gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt
      121 ctttgacctg atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt
      181 cgatggctgc acgattgaga gcaatataag cctcggtgga aatcgcctgc tcgccgcctt
      241 ctttggcact gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt
      301 ttgccgccgc catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt
      361 ccagaatatc aaagtccgct tcacgatagg cgagagcttc ggatttcacg cgggcgatat
      421 ctttcgccat cagcgagacg atgattttag gtgcgcccgc accaatgacg agatctttta
      481 cagttacggt tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc
      541 ccatgacctg tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt
      601 cagccccttg ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgtg
      661 cctgctgcaa taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg
      721 gatgtaacag actgatatca ttaaccaatg attcattctc aaggggtttc atacctactt
      781 ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg
      841 cgagatcggt atccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga
      901 gggctttatc gaagaactca tcccgacggt taaagagttt aatttctttt aaaccttcaa
      961 ttgccccctg cgcgccaatc gccgttgagg cacctccggc ccctaacagc accatcgttt
     1021 tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt
     1081 tatagccacg cagatagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg
     1141 ctgctggcgt taattcatca acatattcac acgccagttg tttgttcggc atcgacacac
     1201 cagttccgcg cattttgagg gcttttaatc cttcaattgc tccgggaaag ctatcgttat
     1261 ccacttcgaa ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattctgca
     1321 tttcaggcga taaactgtgg cggataggat aagccatcaa tccaatcaat tcgtattttg
     1381 cggtaacatc cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct
     1441 aaatttgcgt tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg
     1501 gctaaaaaca ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat
     1561 gatatattgc aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc
     1621 cagtccaccc atcatcatat aaatactggt gactttggct ttgcttttgg ggaaaaactc
     1681 tgacatgacc gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa
     1741 ggcgccggca ttacacacca gtggagaagg gtacaggtag ataatggcag ctgttattgt
     1801 tgccagcgca gagttaaata cattagccca gatgggccgg accatttttt tcagtagtgc
     1861 ggcaaaaata aagacacaga ccaacgagcc catactgtaa taagagatgg tttttaatgc
     1921 ctcagcttct gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac
     1981 ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac
     2041 taaaggtttg ctgttcattt gcggtaattc attagctacg ctggcgtcca ccaactggct
     2101 ggggaatttg cttttcaaca gcatcagcgt gatcagtaca aacagaatac ccggaataat
     2161 cagcccatag ccgtaccaga tattattgag caacatatag ctcaccagca ttgggtagaa
     2221 catttgccca aatgacacca tcgctttaac cagtatgacc gccgaaccag aggctttcgg
     2281 aaagcattcc atgagcgcgg ggtagccacc cgtatccagc gctgagttag cgatacctac
     2341 gcacactgcc agaccgtagg cgagagttaa attcgggcaa gcgggaatac caaagaagaa
     2401 tagcagatac attattactg ccattaatat caccgcccga cgaccaaact tatcggagat
     2461 cacaccgaag aataaaatac tgatcaatcg ccccaaaccg ataccggaaa ttaaataggc
     2521 aatgcccgcg ttgtcagtgg aaaacttttc tgacagagat gtcatatttt gggcaagcgt
     2581 aataacacta atgccgtgca ggaaatagct gaagtaaata caaagaacag ccaggataaa
     2641 tggcgtgctg aaagataatt tcgttaccgt acgtattgtt ctccgacggt ttacataatt
     2701 actatactga agatgatttt ccctgggtac atggcataaa gtttatgttt ccaggaatat
     2761 tgcatgtcat agtttgtttt tgatcgcgat tttccttagt tagcgcagaa agagttattt
     2821 atccgtaaga tcttgcatga cctgaaaaga atattgcatt acccacccgg agcgacattt
     2881 tcctttagcg agtgatgccg cgtttttttg cggctgcgta agccgataaa cagtgccagt
     2941 aaaaaaccga tggcagtatc gaaccacata atatcggcaa tacttctttg ggacagatga
     3001 gccgtaatca acggaatagt aaaggtcgca atactgcccg tactgtaata gatccctgta
     3061 gctttacctt tagcgtaagg gaaacgttca gccattaacg tcaggccaat ttgcacaaca
     3121 cctccagcag aggtaaaacc aatgacaaaa gcaaatatta tcaccacata aaatgtggga
     3181 tgcaggcaga cggtaaacag agcaataaat gagataaagg tgtacagcat cagtaatgtt
     3241 gttgggcgaa cggtattacg aatgagtgga gcggtaataa atacacaaag cagcgaaccc
     3301 acggtgtaga tactgagtag tttgatcgac atagtgtatg acatgcctgc aacaaattgt
     3361 ccgtactgtg ccagccactg gctaaccaga taaaacgttg ccattgagat atagccatat
     3421 aaggtataac tggctaaatc gataattgaa cagcgatgtt ccgtagagct ggtggttttc
     3481 tttatgacag gtaagcgacg acccggatgg ggtgggaacg tacaacggta taaaaacaga
     3541 gcgttaataa acataatgcc tgcagcaatc ataaaggacc aaccgaacca cagttcagcc
     3601 cacactaaca ggctaatgat tagcggtaat aaaaattgtc cgctggaaac aaatgcttta
     3661 attaaaatat tggctgtccc aggtgagcgt ggaaaagctt ccatcaaact gggataagtg
     3721 cctgcatcga gaaaactgtt tgccattccc gccagaaagc caaaaacata agcgataatg
     3781 atgttattgg tctgcaggat gtcaaaaaag aaggccatat agcagcacat cccgagcatg
     3841 ataaaagggc ggcgaccaaa gcgatcggat aataatcctg caaaaagcaa gacacttaat
     3901 cgaccaatgc ccagcgatga gataactatc gagacacccg cggcattagt ctgccaaagt
     3961 gtctccagcg aggccatatt caggctcatc aaaatgacgc ccataccatg caccaggtaa
     4021 ttaaaataca acccaagtgc ggtagggtaa tagggatttt tcataggttc tacttagcaa
     4081 aggtgcttta aaaagtgtag cgatgttgtg tcagtatcca ttcattattg ttgtaacaac
     4141 gacaattcct ttctgtggaa aaattaacaa agccgctcca gatcgtgtgc atataattca
     4201 gcggctaccg attgatagat cttccagtca ataaaattgc catcagggta gacttgttga
     4261 aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc cgatgcggtt atttattttc
     4321 tctatgatgg catgaagatg tttttttcgt tgctgacgca tagccaacag ttgctcgact
     4381 acacaatcag gaatggcgca tttgccattt tcccactgac gccagctttc actgtcaccg
     4441 gtctgggcaa tccaggtcgc gcattcatca atagtcatgg caaaaatatg gcggagtgcc
     4501 tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa taaacaataa aatataaata
     4561 acattatcag cctgctgacg gcttgaagag agtcacagta tcttgtgcaa cattattgga
     4621 aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga
     4681 tgggaaaaga ctacacacag caattcgttg tttcactcag gggatttcca tgcttataaa
     4741 tcaatatgtt gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc
     4801 agattcgtct cattagttta aaaactgtaa agaatttgcg acaggtacta ccagctgaat
     4861 ggcggtaagc aggaaaatat ggggaagagc aagcaaatat caattagcgg tgattaacaa
     4921 tcaggcggca gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt
     4981 cttcgagaat ttcttccggt tggtccgtcg gtggcgggac ttcttccacc cacgcggcaa
     5041 acagacgcca ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag
     5101 caatcaaacc accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc
     5161 gaattaacat tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca
     5221 ataccgttcc ccaggtggta tcgccggtcc agtagagcca gataatcgcc ggaatcagta
     5281 ccggcaacgg gccaagctgg acaaggcagg agaggatcat taacaccgtt agcaacgttg
     5341 cataaggtac gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg
     5401 tcaccaccac acccagcgcc accgcgcgga tagcctgtgc cgccagcagc acggcggcat
     5461 ctccgcgaac gcctgccaga cgggttgcaa aatggcgaat gccttgtgcc acctgttcac
     5521 cgcgccagta cagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc
     5581 cgatatgcgc cgcctgccca acgaaccagg tggtggtggt gccaatataa gggcggactt
     5641 tcgccatgat cgccgtaccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg
     5701 cgccaatcac cggaatggta ttaagccacg ccagatcggg taacgtcatg tcgccgctgg
     5761 aaatggcttt aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag
     5821 ggatgataaa caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc
     5881 caaacatgat cttttgcaaa cgtaacaata ccggccaggt ggcgataacc accgtaccgg
     5941 cccatgcaaa gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg
     6001 ctaaaaacag caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca
     6061 taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact
     6121 cacctgcata atgatgagca atttcagcgg tttttaacag gccgattctg cccttaattc
     6181 tgatgggcgt gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac
     6241 tcgtcgtatt tcaagttgca tgtgctgcgg ctacgttcgt tcaccccagt cacttactat
     6301 tgtaagctcc tggagttcac tcgcttgccg ccttcctgca actcgaaatc catagagtaa
     6361 ataaacatca cctttcagtg cgctattgca gccgcctgaa agaatagtgt atcgacctac
     6421 atcacagacc gcaggaaagg gtcaatataa tgattccaca gatttccctg gcacccggcg
     6481 tcgttcaact ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgatg
     6541 cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc
     6601 tccccgatgc ggtggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg
     6661 ccgcgcagga acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta
     6721 acggtcaggc gctcaaccag gggattattg ttgatatgtc ccgccatatg aaccgcatca
     6781 tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagaccaac
     6841 tcaatcagta cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc
     6901 gggcaacgct cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg
     6961 gcaaaacgtc agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg
     7021 atacgcaacc tttacccgtc gagctggcgg aaacgctggg taaatccaat accacaatcg
     7081 ggcgaattta taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca
     7141 acttccccaa acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg
     7201 agatgaccga gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta
     7261 ttaccgaagc gcggctggat attacgcgct tgcctaaagt gcgccgtctg gtgaatgtca
     7321 aatatgactc ttttgactct gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc
     7381 tttcggtaga gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc
     7441 attccgtcag cgagttgatt accgatatgc ctgacaaaga gatgctcgga ctgaacattg
     7501 tggaatttgc tggtgatgat gaggcgctga ttgacgagcg agttaatgca ctctgtgtgc
     7561 ggcttgatga gctgattgcc agccaacagg caggtgtgat tggctggcag gtgtgccgcg
     7621 agctggcggg ggttgaacgt atctatgcga tgcgcaaaaa agccgttggt ctgcttggca
     7681 atgccaaagg tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac
     7741 atctggccga ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg
     7801 gtatgttcgg tcacgtcgac gcaggtgtct tgcacgtccg tccggcactg gatatgtgcg
     7861 atccgcaaca agagattttg atgaagcaaa tctctgatga cgtggtggcg ctgactgcga
     7921 aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg
     7981 cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc
     8041 ataaccgact caacccaggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga
     8101 aagtggacgc ggtgaagcgc ggtacattcg atcggcagat ccccattgcg gtacgccagc
     8161 agtggcgcgg tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta
     8221 gtccgatgtg tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg
     8281 caacgctggt gcgtgaatgg ctgcgcttgt tggcggatcg cggcgttgac ccactcaaac
     8341 tggaacaaga actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca
     8401 atagctggca tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt
     8461 cgggctgtct ggcctgtaaa gcttgttcga cccagtgccc catcaaaatt gatgtgccgg
     8521 agtttcgctc tcgttttctg cagctctatc acacccgtta tttacgcccg cttcgcgacc
     8581 acctcgttgc tacggtcgag agctacgcac cgctgatggc acgcgcgccg aaaaccttta
     8641 acttcttcat taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg
     8701 atttgccact gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa
     8761 acatgacgct ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgttgg
     8821 tggtgcagga cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc
     8881 tggttgaaaa attaggtttc cagcctgtgt tactgccatt ttcgccaaat ggcaaagccc
     8941 agcatatcaa aggctttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc
     9001 tcaaccgtat ggctaagctt gggatgccaa tggtgggcgt cgatccggcg ctggtacttt
     9061 gttatcgcga tgaatataaa ctggcgctgg gcgaggaacg tggcgagttt aacgtcttac
     9121 ttgcgaatga atggctggca agcgcacttg agtcacatcc agtggctaca gtcagcggtg
     9181 aatcatggta tttctttggt cactgtaccg aggttaccgc tttgccgggc gcgccagcac
     9241 aatgggccgc gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct
     9301 gcggcatggc agggacttac ggacatgaag cgaaaaacca tgaaaattcg ctcgggatct
     9361 atgagttatc ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg
     9421 gatattcctg ccgtagccag gtaaaacggg ttgaaggcac gggggtacgc catcctgtgc
     9481 aggctttact ggagattatt aaatgatatg gaaacggaaa atcaccctgg aagcactgaa
     9541 tgctatgggt gaaggaaaca tggtggggtt cctggatatt cgctttgaac atattggtga
     9601 tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct
     9661 gcatggagga gcatccgtgg tactggccga aagtatcggt tccgttgccg gttatttatg
     9721 taccgaaggt gagcaaaaag tggttggtct ggaaatcaat gctaatcacg tccgctcggc
     9781 acgagaaggg cgggtgcgcg gcatatgtaa accgttgcat ctcggttcgc gtcaccaggt
     9841 ctggcagatt gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac
     9901 cgccattttg tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct
     9961 gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac
    10021 tcaacttgat cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc
    10081 acctgcccaa tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac
    10141 tctctcttct gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg
    10201 atcctgactt aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg
    10261 atgaccttct taatactgct tatctaccgc ctgatattgt tcaaaaataa tactaatcgc
    10321 agtttgtgtt aaaacggcgg gttagcttta tgagattatt cttatcgccc cttcaagagc
    10381 taagccactg agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt
    10441 cgcacagact taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa
    10501 tagtcatttt caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata
    10561 accattatct aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg
    10621 gctatgctta ttaaaagcag gcaaaaagaa tgttttacgg taaagcccct gcgtttgctg
    10681 ggttgaactg ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag
    10741 gtaaatagat ggacatgcat tcaggaacct ttaacccaca ggatttcgcc tggcaaggct
    10801 taacgctgac acccgcagcg gcgatacaca tccgcgagct ggtggcaaag cagccaggta
    10861 tggtcggcgt gcgcttaggt gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg
    10921 acagtgttag cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt
    10981 ttgtcccgct gcaatcgatg ccgtttattg atggcacgga agtcgatttc gttcgtgaag
    11041 gacttaatca gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg
    11101 aaagctttag ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa
    11161 aacctggacc ggcggcccgc tgaactataa agaaggattc ttcacccagt tagccaccga
    11221 tgagctggca aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga
    11281 gccggagtgg atgctggagt ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga
    11341 accgcactgg ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc
    11401 agcaccatcg tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca
    11461 aactggcgcg aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt
    11521 tcccgtgcgg gaaggcaaag aggtggcggt ggatgccatt ttcgactctg tttcggttgc
    11581 caccacttat cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc
    11641 gatccacgat cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga
    11701 caacttcttt gcagcactta atgcggcggt agcctctgac ggtacgttta tttatgtgcc
    11761 taaaggcgtg cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac
    11821 cgggcagttt gagcgcacaa ttctggtggc cgacgaagac agctacgtca gctacattga
    11881 aggctgttcc gctccggtgc gtgacagcta tcagttgcac gcggcagtgg tggaagtcat
    11941 catccataaa aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa
    12001 caacaccggc ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag
    12061 caaaatgtca tggacgcaat cagaaaccgg gtcagcgatt acgtggaaat atcccagctg
    12121 cattttgcgc ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca
    12181 tcagcaagcg gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgaccat
    12241 tatctcgaaa gggatctctg ccggacatag tcagaacagt tatcgcggct tagtgaaaat
    12301 catgccgacg gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc
    12361 taattgtggg gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga
    12421 gcacgaggca acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg
    12481 gatcagcgaa gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc
    12541 agaactgccg ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca
    12601 cagcgtcgga taaggaataa acatgttaag tattaaagat ttacacgtca gcgtggaaga
    12661 taaagctatc ctgcgtggat taagcctcga cgttcgtccc ggcgaagttc acgccattat
    12721 ggggccaaac ggttcgggca aaagtacctt atcggcaacg cttgccgggc gcgaagatta
    12781 tgaagtgacg ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga
    12841 agatcgtgca ggcgaaggca tctttatggc cttccagtat ccggtggaga ttccaggcgt
    12901 cagtaaccag tttttcctgc aaacggcact taatgcagtg cgcagctatc gcggccagga
    12961 aacgctcgac cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat
    13021 gccggaagat ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg
    13081 caacgatatt ttgcaaatgg cggtgctgga accggagtta tgcattcttg atgagtcgga
    13141 ctccgggctg gatattgacg cattaaaagt ggtcgccgat ggcgtgaact cgctgcgtga
    13201 tggcaagcgc tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc
    13261 tgattacgtt catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt
    13321 caaacaactg gaggagcagg gttatggctg gcttaccgaa cagcagtaat gcgctgcaac
    13381 agtggcatca cttgtttgaa gctgaaggga caaaacgctc accgcaagca cagcagcatt
    13441 tacaacaatt gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc
    13501 cgctggaagg gctgaccaat agtcagtttg tcagcattgc gggagatata tccccacagc
    13561 agcgtgatgc cttagcgtta acgttagacg ccgtgcggct ggtgtttgtc gatggacgtt
    13621 acgtgcccgc gctgagcgat gcaattgaag gcagcggata tgaagtgagc attaacgacg
    13681 accgtcaggg gttacccgac gctattcagg cggaagtgtt tctgcatttg acggaaagcc
    13741 tggcacaaag cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc
    13801 tgttaatgca tatcacccag ggcgtggcag gtgaagaggt gaacactgcc cattaccggc
    13861 atcatctgga tctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctta
    13921 atgatgctcg tcattttacc ggggcacggt tcactatcaa tgtcgcagcg aacgcccact
    13981 tgcagcatat caagctggcg tttgaaaacc cggtcagtca ccactttgcc cataacgatt
    14041 tgttgctggc tgacgatgcc accgcattta gccatagttt cctgctgggt ggcgcagtgt
    14101 tacgacacaa caccagtacg caactcaatg gcgaaaacag cacgctgcgg atcaatagcc
    14161 tggcgatgcc ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag
    14221 gtttttgtaa cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat
    14281 ttaacggttt gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca
    14341 ataatctgct gatgggcaaa ctggcggaag tggatacaaa accgcagctg gaaatctatg
    14401 cagatgatgt gaaatgcagc cacggcgcga cggtggggcg tattgatgat gaacagatgt
    14461 tctatctgcg ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct
    14521 ttgctgccga actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctggccc
    14581 gaatcggtca acggctgcca ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc
    14641 cgactttccg gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc
    14701 cgccagtgca cagaaaccga gccaggtgat tgacgccgag gccgagtttt atcgtcatgg
    14761 ctacgcggcg gtgcatcgcg gtattcatac cttaagcgcc caggcgaccg agaaaatgga
    14821 gaacgtgcgc aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt
    14881 tgtccgcggc acgacggaag ggatcaatct ggtcgccaat agctggggca acagcaatgt
    14941 gcgggcgggc gataacatca tcatcagtca gatggagcac cacgctaaca ttgtcccctg
    15001 gcagatgctt tgcgcacgcg ttggcgcaga gctgcgtgtg atccctctca accccgacgg
    15061 tacgctgcaa ctggagacgc tgcctacgct gtttgatgag aaaactcgcc tgctggcaat
    15121 tactcatgtc tccaacgtgc ttggcacaga aaatccactg gcggaaatga tcacgcttgc
    15181 gcaccagcat ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt
    15241 ggatgttcag gcgctggatt gcgactttta cgtgttctcc gggcataaac tgtatggccc
    15301 caccggaatt ggcattcttt atgtgaaaga agccttgttg caggagatgc cgccgtggga
    15361 agggggcggt tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc
    15421 accatggcgg tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc
    15481 gctggagtat gtttcggcgc tggggcttaa tagcatagcc gagtatgaac agaatctgat
    15541 gcattatgcg ctatcacagc tggaatctgt accggatctc actctctatg gccaacaaaa
    15601 caggcttggc gttattgctt ttaatctcgg taaacaccac gcctatgatg ttggcagttt
    15661 tctcgataat tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc
    15721 ctattacaac gtccctgcga tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga
    15781 agtggagcgt ctggtgaccg gcctgcaacg tattcaccgt ctgctgggat aacagggagg
    15841 cactatggct ttattgccgg ataaagaaaa attgctgcgt aattttttac gctgcgccaa
    15901 ctgggaagag aaatatctct acattattga gctgggccag cgtctgccag aattacgcgc
    15961 cgaagacgga agcccacaaa atagcattca gggctgccag agtcaggtgt ggattgtcat
    16021 gcgccagaat gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa
    16081 agggcttatt gcggtcgtct ttattctcta cgatcagatg acgccgcagg atattgtcaa
    16141 tttcgatgtg cgtccgtggt ttgaaaaaat ggcgctcacc caacatctca ccccatctcg
    16201 ttcacaaggt ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta
    16261 aactaaaggg acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact
    16321 gaccggcaca ggagtgtttt ggtttcaggt gaacataagc accatccgac gtttcagcgt
    16381 gagtctccgg caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc
    16441 ttatcggcgc ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa
    16501 ccggaagccg actggttggg caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc
    16561 ttcaggctat tgcccgacgt tttgatactg cagcaatgtt gatccttgaa gccaataaca
    16621 ctatcgcccc ggtgccaaaa cctggtacga cgataactat tccttcacaa ctgttattac
    16681 ctgatgcacc gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc
    16741 cgccgggaga aaatattgtg caggtttatc caataggtat tggattgcag gggctggaaa
    16801 cgccggtgat ggaaacgcgt gttgggcaga aaatccctaa cccaacctgg acgcctacgg
    16861 caggcattcg tcagcgttcg ctggagcgtg gcattaaatt accgccagtc gttcctgccg
    16921 gaccaaataa cccgctagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc
    16981 tcattcatgg caccagtgcg ccggacagcg ttggtttgcg cgtcagttca gggtgtattc
    17041 gcatgaatgc accggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga
    17101 aagtgatcaa cgaaccggtg aaatattccg tggaacctaa cgggatgcgt tatgttgaag
    17161 tacatcgacc actatcagca gaagaacagc aaaacgttca gacaatgcca tacacactgc
    17221 cagcaggctt tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag
    17281 cgttgtatcg tcgggcaggg tatccggttt cggtgagcag tggagcaact cccgcagcca
    17341 gcaatgcgcc ttcagtagag tcagcgcaga atggtgaacc agagcaaggg aatatgttac
    17401 gcgcgacgca gtagtggtaa atagcagaca aaaaaatggc gcacaatgtg cgccattttt
    17461 cacttcacag gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag
    17521 cacgagctgc gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc
    17581 tcagctggtc aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag
    17641 cgttgctgga gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt
    17701 tagtagcttt cattattaat accctctaga ttgagttaat ctccatgtag cgttacaagt
    17761 attacacaaa gttttttatg ttgagaatat ttttttgatg ggaaggcact tatttttgat
    17821 cgttcgctca aagaagcatc gaacgctggg ttttagataa aaaaattgag agaaaacagg
    17881 atgcttccat cggattcatc ttaaataaag tgagattata tagtgaaatc cgatttgatt
    17941 ttttattgct tctggttatc gattaaataa aaaaagcgcc catcagggcg cttcgatata
    18001 caaattaatt cacaaaagca atattacagg acgtgaacag atgcggtgtt agtagtgccg
    18061 ctcggtacca gtgcaccaga aaccataact acgacgtcac ctttgtgtgc cagaccgctc
    18121 tgcagagcca gttctttacc cagacggtag aaatcatcag tagaagtgat ctctttaacc
    18181 agctgcggca caacgccttt gctcagtacc aactgatgag ccgttttttc gttggtggtc
    18241 agtgccagga tggtggcatc cgggaagtat ttacgtactg cgcgagcaga tttaccgccc
    18301 tgggtagcaa ccacgatcag cggagcatcc agtttttcag cagtttcaac ggcaccacgg
    18361 cataccgctt cggtgatgcg cagtttacgg ttgtcattgt tgaactcgag acggctgttc
    18421 atcacgcggt cggtacgttc gcagatggtc gccatgatag aaaccgcttc cagcgggtat
    18481 ttaccttttg cggattcacc agacagcatc actgcgtcag taccgtcgag gatggcgttt
    18541 gcaacgtcac cggcttctgc gcgagtcggg cgtgggtttt tgatcatgga atccagcatc
    18601 tgggtcgcag tgataacgac tttacgtgca cggatacatt tttcgatcat catcttctgg
    18661 gcgaagataa cttcttctac cgggatttct acacccaggt cgccacgcgc aaccatgatg
    18721 ccgtcagagg cttcgaggat ttcgtcgaag ttgttgaggc cttcctggtt ttcgattttg
    18781 gagatgatgt ggatgttttc gccgccgtgc gctttcaggt gctcacggat ttcgataacg
    18841 tcagaacgct tacgaataaa ggaagcagca acaaagtcta cgcctttttc gcaaccaaag
    18901 atcaggtcct gtttgtcttt ttcagccagt gctggcagag caatggaaac gccaggcagg
    18961 ttcacacctt tgttttcgcc caggtcaccg ttgttcagca ctttacagat aactttgtta
    19021 ccttcaatgg cggtaacttc cataccgatc agaccatcgt caaccagtac ggtgttgcca
    19081 acagacaggt cagtagtgaa accttcatac gttaccgcaa ccatttcgct gttgccgata
    19141 acagatttat cagtggtgaa agtaaaggtc tgaccagctt tcagagaaac atcgttaccg
    19201 ccttccagtt tcatggtgcg gatttccgga cctttggtat caagcaggat agcggcggtt
    19261 ttaccagttt tgctcatcac gttgcgcaga ttctgaatgc gctgaccgtg ttctgcatag
    19321 tcaccatgag agaagttcag acgcataacg ttcatgccag cgtccagcat tttagctaac
    19381 atctcttcag attcggtttt cggtccgatg gtgcaaacaa ttttggtctt tttcatgaca
    19441 gtcttagtct ttaagttgag aaggatggga gaaacttgct ttctgggcgc actgccggaa
    19501 agcgaatccg gtattacgaa agtggtggtg cctcgctcta aggataggtg acatcgaaaa
    19561 agcgtgcaga ggaatgtgtg cttgtgtctc agcccaacgt gagtgtctgt ttttgagtcg
    19621 ttgagttcta cagtccaaag tgctgaaacc attcaagagt caattggcgc gcattatacg
    19681 ctgaaattaa tccaaaagga aaacaaaaac agcgtttcaa aaaatataca acttgttaga
    19741 caataaattg ttatcaaaga tttaacgttc cagggaaaag gttacgcaaa aacttcgctg
    19801 tttgcgcaac gtctggtgcc gaagttgtca gtgtgaggat aagtgaaagt tcatccggtg
    19861 tataaatata tcatcgctca attgctacag ttaaaataat ttatcgcgtt atttgtctga
    19921 ttttgtttga tttttgttta attggttaga ttgatatatg ctgata
//
LOCUS       DAJVPT010000078        19795 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVPT010000078 DAJVPT010000000
VERSION     DAJVPT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            40..309
                     /locus_tag="OQM47_002706"
     CDS             40..309
                     /locus_tag="OQM47_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1480801.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            303..668
                     /locus_tag="OQM47_002707"
     CDS             303..668
                     /locus_tag="OQM47_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1480802.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(669..1457)
                     /gene="bioH"
                     /locus_tag="OQM47_002708"
     CDS             complement(669..1457)
                     /gene="bioH"
                     /locus_tag="OQM47_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1480803.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1495..2178
                     /gene="gntX"
                     /locus_tag="OQM47_002709"
     CDS             1495..2178
                     /gene="gntX"
                     /locus_tag="OQM47_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1480804.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2237..2812
                     /gene="nfuA"
                     /locus_tag="OQM47_002710"
     CDS             2237..2812
                     /gene="nfuA"
                     /locus_tag="OQM47_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1480805.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3173..4489
                     /gene="gntT"
                     /locus_tag="OQM47_002711"
     CDS             3173..4489
                     /gene="gntT"
                     /locus_tag="OQM47_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1480806.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4534..6618)
                     /gene="malQ"
                     /locus_tag="OQM47_002712"
     CDS             complement(4534..6618)
                     /gene="malQ"
                     /locus_tag="OQM47_002712"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1480807.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TTTKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6628..9021)
                     /gene="malP"
                     /locus_tag="OQM47_002713"
     CDS             complement(6628..9021)
                     /gene="malP"
                     /locus_tag="OQM47_002713"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1480808.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9633..12338
                     /gene="malT"
                     /locus_tag="OQM47_002714"
                     /pseudo
     CDS             9633..12338
                     /gene="malT"
                     /locus_tag="OQM47_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12381..13397)
                     /gene="rtcA"
                     /locus_tag="OQM47_002715"
     CDS             complement(12381..13397)
                     /gene="rtcA"
                     /locus_tag="OQM47_002715"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1480809.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13401..14627)
                     /gene="rtcB"
                     /locus_tag="OQM47_002716"
     CDS             complement(13401..14627)
                     /gene="rtcB"
                     /locus_tag="OQM47_002716"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1480810.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14808..16406
                     /gene="rtcR"
                     /locus_tag="OQM47_002717"
     CDS             14808..16406
                     /gene="rtcR"
                     /locus_tag="OQM47_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1480811.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16388..17146)
                     /locus_tag="OQM47_002718"
     CDS             complement(16388..17146)
                     /locus_tag="OQM47_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1480812.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17163..17993)
                     /gene="glpG"
                     /locus_tag="OQM47_002719"
     CDS             complement(17163..17993)
                     /gene="glpG"
                     /locus_tag="OQM47_002719"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1480813.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18038..18364)
                     /gene="glpE"
                     /locus_tag="OQM47_002720"
     CDS             complement(18038..18364)
                     /gene="glpE"
                     /locus_tag="OQM47_002720"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1480814.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18554..>19795
                     /gene="glpD"
                     /locus_tag="OQM47_002721"
     CDS             18554..>19795
                     /gene="glpD"
                     /locus_tag="OQM47_002721"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1480815.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLA"
BASE COUNT         4637 a         5472 c         5005 g         4681 t
ORIGIN      
        1 atcttgcatg taacacaaag tagcacttag ggaactgata tgcagacagt gaaattacgg
       61 caacaaggag gagccatgat cgttactatc ccacgagatc ttgccatcga tcttggctgg
      121 tcaccgggca cagagcttac cgtggagaaa aaaggcgata gcgttaattt gcgcgccaca
      181 gagcacaaac cgcgtgggcg tcttaccgtc gcgcagttgt tgagccaaat cgatgaaagc
      241 gaaattacgg agctaaatca gtccacagaa ggatgggctg aagggaaaaa aggtaatgaa
      301 gcatggtaaa ggcacggacg ccacatcgtg gtgagatctg gtattttaac cctgatccgg
      361 ttgccgggca tgaacttcag gggccacatt attgcattgt ggtaacggac aaaaaactca
      421 acaatgtttt aaaagttgct atgtgctgcc cgatttcaac aggggcaaat gcagcacgtt
      481 ccacaggggt gacggtgaac gtcctccccc gtgatacgca aaccggtaac ctgcatggcg
      541 ttgtactttg tcaccagcta aaagccgtcg atcttattgc ccgtggcgct aaatttcata
      601 ccgttgccga tgaaaaattg attagtgaag ttatcagtaa actggtgaat ttaatcgacc
      661 cacaataatt agctctcact taccaccaac accctctgct tcaacgccac cagcaggtga
      721 caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa gatatatgat
      781 tcgctgtgag gccaaagttt atccagcatc ggcaccactt tgcgcggcac cagaccgtcg
      841 agatagccat acaatcgcaa aaacggcatg gacacgtttt gcagcggctg acggagatcg
      901 accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat cggtaacgcc
      961 agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg tttcagtccc catggtttgt
     1021 aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg ctgaaatccc
     1081 gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca aggtgacgac
     1141 gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg gcttgccacc
     1201 agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgttg cagcacggct
     1261 tcggccatat cagcaagtga cagcgcacca aatccccggc tacgcccgaa gccgggcagg
     1321 tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg ccacacttcg
     1381 gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc tttggtctgc
     1441 caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga acatatgcta
     1501 acagtaccgg gattatgctg gctatgccga atgccactgg cgttaggtca ttgggggatt
     1561 tgttcggtct gttcacgcgc cacccgcaca gataaaacgt tatgcccaca atgtggatta
     1621 cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccgcc gccctggcaa
     1681 agactggtca cggttgccga ctatgcgccg ccgttaagtc cgcttatcca ccagcttaaa
     1741 ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta
     1801 cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt tccgttatgg
     1861 cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca gccgttatca
     1921 cgctggttgc actgccaatg ggatagcgaa gccgtcacac gtacacgggc cactgcgacc
     1981 cagcattttc tcagtgcccg gctgcgcaag cgcaacctga aaaatgcctt tcgtcttgaa
     2041 ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac cggaagtacc
     2101 gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc
     2161 ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta aaatagtcaa
     2221 ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact ttgccaaact
     2281 gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc
     2341 taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca ccgacactgc
     2401 cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat acctggaaga
     2461 tgcagagatc gactttgtta ccgaccagtt gggttctcag ttaacgctga aagccccgaa
     2521 cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg gagcgcgtgg agtatatgct
     2581 gcagtcgcag atcaacccac agcttgctgg tcacggtggt cgcgtttcgc tgatggaaat
     2641 caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt gttccatggt
     2701 cgatgtgacg ctgaaagaag ggatcgagaa gcagctgctg aacgaattcc cggagctgaa
     2761 aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact aagttatccc
     2821 ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg
     2881 tgccagacgt tggcacagca ttgctttggc aaatccctcc cttcgtttcc ccgcgataat
     2941 atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa cgccaaatgt
     3001 tacccgcatc attcacatgg gtaccaaaca tactcctgac atctgactac aataattagt
     3061 tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga taattagagt
     3121 ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa tcatgccatt
     3181 agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgct tcaaaatgaa
     3241 cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag gaatgccgct
     3301 ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta gccttgccct
     3361 gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg gcgcacaacg
     3421 tatcgccacc acgctgattg ccaaatttgg taaaaagcac atccagtggg cggtggtgtt
     3481 aaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggcttcgtgc tgatgctgcc
     3541 gctggtgttt accatcgcgg cttccgcgaa tattccattg ctgtatgttg gtgtaccaat
     3601 ggcggctgca ctgtctgtga cccacggctt cctgccaccg catccgggcc cgactgcgat
     3661 tgccaccatt ttcaatgccg atatgggtaa aaccctgctg tacggtacta ttctggcaat
     3721 cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc
     3781 aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg
     3841 cgtcagcgtc tggacctctc tggtgccggt tgtgctgatg gcgatgcgtg cgattgccga
     3901 aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg gtgacccggt
     3961 aatggcaacg ctgattgccg tgctgattgc gatgttcacc tttggtctga accgtggtcg
     4021 ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg cgatgatgct
     4081 gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa
     4141 atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg cctggtcgat
     4201 tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg
     4261 tatcgcggca ccgctgattg caacgacggg tgttagccca gagctgatgg ttattgcggt
     4321 gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc tgttcaaaga
     4381 gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg aaaccattat
     4441 ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat aacactgact
     4501 gccggatgca tcgcatccga catcaaccgc actctacttc ttcttcgctg cagctctgcg
     4561 ccgtctgtcc aaatccttca gcaacttgtt cacgccatca tcggcaaaca tcgactcaag
     4621 cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag gaatattcac
     4681 cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac tgttactgtc
     4741 ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc atcgacatca acgatgcctt
     4801 atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca gcccttgctt
     4861 cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat acagccccag
     4921 ggttttgccc agcgttagat ccccgctctc ccagtaacca cgcagcgttg gcaggtcatg
     4981 tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga acgtcttctc
     5041 atggtcgttt tcgaaataga gcactttgta agagtacaca ccgctgctgc gcagcttacc
     5101 aacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc gatgacgttt
     5161 actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat acgcgccctg
     5221 atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca catggtcaat
     5281 tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg gttcataggc
     5341 acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc ccaacgggcc
     5401 gaggatatcc ggcggcgcgc caaccgatgc tttcaggcaa tatagttcac ggtcacacca
     5461 agtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc caatcggcat
     5521 ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa
     5581 ccagagataa aaatcgacgt catcacgatg ttcttcgcag aactgacgca cttctggtga
     5641 atccacgttc ttatacatct ctggccatgc aggccagccc cagcgcattt cgtcctcttt
     5701 cacttgctgg gcatgtagcg catcaaaggc agcctgccag aacaggctgt cgccctgctc
     5761 tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga aacctttcca
     5821 cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga cccagtcggc
     5881 atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc
     5941 ttcgctaaga tggaaatctt caacggcgtt aacgtcgata taaatcacat tcagccaacg
     6001 gcgagaagac gggctgtatg ggctggcgct ctccggattt gccggataga gcgcatgaat
     6061 cgggttcagg ccaatgaacg acccgccacg ttttgccaca tccaccagca tcgctttgag
     6121 atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa gctgaacgca
     6181 ggcaccccac agcttttgtt tattcagcaa agcctgcggt tcgtaacaac gtttcggggc
     6241 gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgttagcg tgtgataacc
     6301 ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc ctttgtactg
     6361 cgttccttct tcggtggtca gctgccagct atattcgccg ctgccctcca ccaccatcgg
     6421 catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg ccactttcgt
     6481 ggtggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg
     6541 cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg cggcattatc
     6601 cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac gagcctgata
     6661 atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga ggatcgccgc
     6721 gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg cctcaacata
     6781 ggctgcgaaa tccgccatca ccagatacgg atcgccgccc tgtttgccga tactgtgcag
     6841 catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca gctctttcaa
     6901 tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc ctttggccag
     6961 aatagccttc acttgttcca cggtatgacc aaaaataaag atattttctt caccgacttt
     7021 ctcggcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac cattgagcgc
     7081 cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt gttcggagat
     7141 atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca ggaacaccac
     7201 cttcaactta tcgccaacca gtggatcgtt gttgatcacg tcagccactt tgttgatcgc
     7261 aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga agaggaagac
     7321 gcgcggtacg cgatcagcct gcaggttttc acgaatttct ttgtacaacg ccagaatatg
     7381 cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat
     7441 cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca gacagacttt
     7501 attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag cgaatttttc
     7561 cagattgatc agctgatcga gatcgttagc ccactctttt tgcagtgatt tatccaacag
     7621 agccgccagt gcagggttgc actgtttgat ccagcgacgt ggggtaatac cgttggtaac
     7681 gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt tcaccaccag
     7741 atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac acaggttcgc
     7801 catatgcact tgtttatcgt gcaccaccgc cagtttggcc cacacttttt catcgcccgg
     7861 ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa tctgcatatg
     7921 gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag
     7981 ggtatggttg gtgtaagcga aagttttgct ggtgatggcc caggcgtcat cccagctcat
     8041 ctggtgctca tcggtcagca cgcgcagcag ttctggaatc gcgatagttg gatgggtatc
     8101 gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg ccagatgatg
     8161 gcgacgcaaa atatccgcta ccgaacaggc acactggaag tattgctgca tcaggcgcag
     8221 ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt tttccgcatt
     8281 gatgccttgc tgttcggcgc gcaggaaatc accgtcgtta aatttagtta gatcaaacgg
     8341 atgcgcatgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac gatagccgac
     8401 aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt
     8461 catcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc ggaaccacgg
     8521 gtagttactg cgatgccagt catccggcac ttcaacctgt ttgccatcga caaaagactg
     8581 gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac cgacagttgc
     8641 cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca gcgccgggtc
     8701 gatctcttct tccagcaggt cagtcaggtt gatgtcataa gccttcaacg aatcctgtac
     8761 atcctgatac cagccgagat tcaacaggtt gttgcccgtc agacgaccaa tcaaaaactc
     8821 cattgagatg tagttaacat gtcgctgatt cgccaccggt ttggcgaatg gctgagcacg
     8881 cagcatttcg gccagtgctt cactcactgc cagccaccac tggcgaggag tcatttcagc
     8941 cgcagaattt aagccataac gctgccactg acgtgaaagc acttcctgaa attgcttatc
     9001 gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac acaacgttat
     9061 cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta ggcagggagg
     9121 agttgcgggg atgagcaagg aaatgtgatc tcgaccactt aaagctagtg caaaccacag
     9181 gattagcagc aaatcaatgc catacagcgc agaaaatctg tatctaagtg caaaaaatgg
     9241 ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag gagtgaaaca
     9301 gtttcacaag taaaatatcc agtgtgctcc atctcattct taatagattt attaagatca
     9361 tctttttaga tggcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga
     9421 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
     9481 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
     9541 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
     9601 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc
     9661 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
     9721 acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt
     9781 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc
     9841 agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc aacggtcact
     9901 gtgcgatatg tgagacgatg gagcaaaaac ggcaatatgc cagcctgacg tcactcttcg
     9961 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
    10021 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
    10081 cagaaaatct caccctagtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc
    10141 tgcgtgttcg tgaccaactg ctggaaattg gcagtcagca actggcattt acccatcagg
    10201 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca gaaagcagtc
    10261 ggatttgcga tgacgtttcc ggttgggcga cggcactata gctaatcgcc ctctccgccc
    10321 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
    10381 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
    10441 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg
    10501 gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc
    10561 ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc
    10621 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
    10681 gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
    10741 cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac
    10801 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
    10861 agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca
    10921 ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcatgcag
    10981 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatcaggat gaagcggaac
    11041 ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc cgcattgtgg
    11101 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
    11161 tagtgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga
    11221 gtttaattca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
    11281 cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg ccaatgcatg
    11341 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
    11401 aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc
    11461 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
    11521 gccagttgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
    11581 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
    11641 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
    11701 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
    11761 ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
    11821 acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
    11881 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg
    11941 aaggcgaagc gatggcgcaa caactgcgcc agctgattca acttaatacg ctgccggaac
    12001 tggaacagca tcgcgcgcag cgtattctgc gagaaatcaa tcaacatcat cggcataaat
    12061 tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac
    12121 taatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atctactccg
    12181 gttacagcaa tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc
    12241 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg
    12301 cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg
    12361 atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc
    12421 aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat
    12481 gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag taccagttgg
    12541 tcggcgagat attcccccac cgccgctggg cttgccaggt agcgtttcac ctctttcact
    12601 aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca
    12661 gtgatatttt cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc
    12721 aggttatgaa tattctgttc atgcagggaa aaaatccccg ccagtgtagc gatttcacgc
    12781 tcagcaacat ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc
    12841 ccgcgctcgc caagttgcaa ggtgctaaac gatgccacgg gcgagacttc cgttgccacc
    12901 acaccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
    12961 attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg cgccgacggg
    13021 ttatcggtgc cgccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
    13081 agcaccgttt gcagcaccag cgtacaactt ccggcgctac cgatagcaaa gcggtaatcg
    13141 ccgccgcgca cggtgccagg ccggaagacc agacgctgcg atcccagctc cgcgccttcc
    13201 accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg gcgcagcagc
    13261 cccggtttcg cccgtccggc tcgaatgcca gtgatggtaa atggctggcc ggttatcatc
    13321 gacaggctca gcgccgagcg caggatctgc ccgccgcctt cgccctgtgc gccatccagc
    13381 gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag ggtgtagata
    13441 acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata cgccatcggg
    13501 atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc acgaatttgg
    13561 tcttccacgc tgaacagttt tttcgcttta gttcggctca ttacccgccc ggcaccgtgg
    13621 ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct ttttgctccc
    13681 atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcacc tttacgcgtc
    13741 acgtagatct cttcaccaaa gagctgttct ttttgcacat agttgtggtg acagttgatc
    13801 tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaacgcc
    13861 gttaccacat tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg cgcccaggcc
    13921 acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc cagatcacgc
    13981 gacggcaacg tctcaagttg gtcctgcatc tctttttgcg ccagatcgat aaagtaagtc
    14041 ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac ctgctccgac
    14101 tcatcaaggc agatttcaat aaagtggtta cccgttccca gcgttcccag gtgtttatag
    14161 ttgttagtgt taaggaaacg cggatatttt tgcgttaacc actgataacc ggcctccagc
    14221 tcagcccatt tcgcatcgac gttaaaaggc gggttttccc atgcgccttt atcacgttta
    14281 caacgcccgg tagtacgccc gtgtggtacg gccgtttcaa tcgcttgacg cagctccgcc
    14341 aggttttcag gcaggtctgc cgccgttaac gcggtacgca gcgcgttcat tccacagcca
    14401 atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg
    14461 gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc
    14521 atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac gcctttggtc
    14581 cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct
    14641 tttcgttgtt tgcggtcctg ataattgcaa ccgtcgtgcc agaaaattaa ataagcagat
    14701 taatttattg tttctaaaaa gattatatct ttatatccgt aaacggagat ttcccgcaaa
    14761 gccgatttac cgataatgaa atatcgcctt ttataaggat atctaagatg cgtaaaacag
    14821 tggcttttgg ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt
    14881 caaaatggcg tccgtcactc tgtatatacc agcaagaatc gttggtcatc gatcgactgg
    14941 aattattgca cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca
    15001 gcgtttcgcc agaaacagaa gtggtgggcg ttgagattga actgcataac ccgtgggatt
    15061 tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa
    15121 aagaagacta tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc
    15181 tgctggcgga agcgcgttat ctacccgccc ggttgataca atcttcaact ccgcgcaaaa
    15241 aagaacagcc gcgcggccca ggtgaagtga cgattatcga tctcgattta agccgttata
    15301 acgccatcgc cagccgcttt gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg
    15361 gtattgccac gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga
    15421 ttaaatcccg cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg
    15481 cgcgacgcat cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag
    15541 tgaactgcgc caccctgcgc ggcgataccg cgatgtcgac gctgtttggt catgtgaaag
    15601 gcgcgtttac cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc aacggcggaa
    15661 tgttgtttct tgatgagatt ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag
    15721 ctattgaaga gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc
    15781 agcttatcgc cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaattccgcg
    15841 aagatctgta cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc
    15901 aggaagatat tgaaccgaac ctggattatg aagtggagcg ccacgccaca ctcactggtg
    15961 acagcgtgcg ttttaacacc gaagcgcggc gtgcctggct ggcctttgca acatctcccc
    16021 aggcaacatg gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct
    16081 ttgccactag cggacgcatc actctggacg tggtggaaga tgagataaac cgtctgcgct
    16141 ataactggca ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa
    16201 acatcgatct cttcgaccgt atgcaactgg aacacgttat cgctatctgc cgccaggcaa
    16261 agtcgctttc tgctgccgga cgacagcttt ttgatgtttc gcgccagggc aaagccagcg
    16321 tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg
    16381 tgcaggatca gcacagctcc agttgaatat ggtggtccgt cagcacctgc atcacgctta
    16441 ctggcggcgg ggcgtcggtg tagacggcat caaccatgct gatgctcccc atattgacca
    16501 tcgcattacg gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa
    16561 taatggcgcg cttggtacga acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt
    16621 cgatgccgct gatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg
    16681 tcgcttcgcc aatgatcccg ccatcgcggc tacgtaattc gccaccggcg agaataatgc
    16741 ggaaatcttc ttttaccatc aacgtgttag caacgttgag attgttggtg acaatgcgca
    16801 aattgctgtg attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa
    16861 acagcgtcga gccattgggg atttgctccg ccactttgcg ggcgatgcgc tctttttctt
    16921 cggtctgggt ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg
    16981 cgccgccatg atggcgcagg atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa
    17041 tagtctgcgg gctgacggag aagtgctcca ccagttcctc ggtactgaca taaccctgct
    17101 gtttaaccag ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg
    17161 aattattttc gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct
    17221 aatccggcga tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg
    17281 acaatccaga tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga
    17341 tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg
    17401 ccaaaccacg gcccgctgaa tttttgctgc atatagccgc ttaacagggc gctgataagc
    17461 gtaatgacaa ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc gagataccac
    17521 caccaaagca ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag
    17581 taacgccaga actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact
    17641 tcctgatcgc cgagaatttg catggcaata aacaccacca cgcaggcgat catcatcacc
    17701 caggtcaccg gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc
    17761 aggccactgc cggtatggcc tgccagccag ctggccgcca gataacgcgg atctgccggg
    17821 ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc
    17881 cagacatcgc tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa
    17941 tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct
    18001 gtcgcttatt ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg
    18061 cgggaaactg tcgctgccag gcttcaaagc cgccgtcaat gctatagacc acatcgtagc
    18121 cctgttgcag cagatactgc gccgcccctt tgctgctatt gccgtgataa cacatcacca
    18181 tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat
    18241 ggaaagcctg caccgcatgc cccatagcga aactctgtgg atcgcgaata tcgaccagca
    18301 ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat
    18361 ccatgcgtct ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa
    18421 gcggcagata aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc
    18481 caaagcgcga ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt
    18541 gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc
    18601 tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga
    18661 tctcgcttgc gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct
    18721 tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa
    18781 aatggctccg catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg
    18841 cccggcgtgg atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag
    18901 cctgccggga tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa
    18961 gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc
    19021 ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg
    19081 ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag
    19141 ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga
    19201 ggggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt
    19261 gccgcgcgtg catacccaaa agcaggccta cattctgcaa aacgaagata aacgcattgt
    19321 gttcgtgatc ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa
    19381 aggtgatccg aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta
    19441 taacgcgcac tttaaaaagc agctaagccg tgacgatatc gtctggacct actccggtgt
    19501 gcgtccgctg tgtgatgatg agtccgactc gccgcaagcc attacccgtg attacaccct
    19561 tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac
    19621 cacctaccga aaactggcag aacatgcgct ggaaaaactg acgccgtatt atcagggtat
    19681 tggcccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga
    19741 cgattacgcc gctcgcctgc gccgccgtta cccgttcctg actgaatcgc tggca
//
LOCUS       DAJVPT010000079        18812 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVPT010000079 DAJVPT010000000
VERSION     DAJVPT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            252..1349
                     /gene="mltA"
                     /locus_tag="OQM47_002722"
     CDS             252..1349
                     /gene="mltA"
                     /locus_tag="OQM47_002722"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1480816.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1587..2393
                     /gene="tcdA"
                     /locus_tag="OQM47_002723"
     CDS             1587..2393
                     /gene="tcdA"
                     /locus_tag="OQM47_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1480817.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2444..2887)
                     /gene="csdE"
                     /locus_tag="OQM47_002724"
     CDS             complement(2444..2887)
                     /gene="csdE"
                     /locus_tag="OQM47_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1480818.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2887..4092)
                     /gene="csdA"
                     /locus_tag="OQM47_002725"
     CDS             complement(2887..4092)
                     /gene="csdA"
                     /locus_tag="OQM47_002725"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1480819.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4284..4511
                     /locus_tag="OQM47_002726"
     CDS             4284..4511
                     /locus_tag="OQM47_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1480820.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4862..5779
                     /gene="gcvA"
                     /locus_tag="OQM47_002727"
     CDS             4862..5779
                     /gene="gcvA"
                     /locus_tag="OQM47_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1480821.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5798..6193
                     /locus_tag="OQM47_002728"
     CDS             5798..6193
                     /locus_tag="OQM47_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1480822.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6186..7286
                     /gene="rlmM"
                     /locus_tag="OQM47_002729"
     CDS             6186..7286
                     /gene="rlmM"
                     /locus_tag="OQM47_002729"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1480823.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7330..8061)
                     /gene="fucR"
                     /locus_tag="OQM47_002730"
     CDS             complement(7330..8061)
                     /gene="fucR"
                     /locus_tag="OQM47_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1480824.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8119..8541)
                     /gene="fucU"
                     /locus_tag="OQM47_002731"
     CDS             complement(8119..8541)
                     /gene="fucU"
                     /locus_tag="OQM47_002731"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1480825.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8543..9962)
                     /gene="fucK"
                     /locus_tag="OQM47_002732"
                     /pseudo
     CDS             complement(8543..9962)
                     /gene="fucK"
                     /locus_tag="OQM47_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10071..11846)
                     /gene="fucI"
                     /locus_tag="OQM47_002733"
     CDS             complement(10071..11846)
                     /gene="fucI"
                     /locus_tag="OQM47_002733"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1480826.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEASACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11879..13195)
                     /gene="fucP"
                     /locus_tag="OQM47_002734"
     CDS             complement(11879..13195)
                     /gene="fucP"
                     /locus_tag="OQM47_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1480827.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13742..14389
                     /gene="fucA"
                     /locus_tag="OQM47_002735"
     CDS             13742..14389
                     /gene="fucA"
                     /locus_tag="OQM47_002735"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1480828.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14417..15565
                     /gene="fucO"
                     /locus_tag="OQM47_002736"
     CDS             14417..15565
                     /gene="fucO"
                     /locus_tag="OQM47_002736"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1480829.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15618..16373)
                     /gene="xni"
                     /locus_tag="OQM47_002737"
     CDS             complement(15618..16373)
                     /gene="xni"
                     /locus_tag="OQM47_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1480830.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16484..17851)
                     /gene="sdaB"
                     /locus_tag="OQM47_002738"
     CDS             complement(16484..17851)
                     /gene="sdaB"
                     /locus_tag="OQM47_002738"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1480831.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17909..>18812)
                     /gene="sdaC"
                     /locus_tag="OQM47_002739"
     CDS             complement(17909..>18812)
                     /gene="sdaC"
                     /locus_tag="OQM47_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1480832.1"
                     /translation="HQLGMTPPPRAILSLILIVGMMTIVRFGEQMIVKAMSILVFPFV
                     GVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVMVFSFNHSPIISSF
                     AVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPADLAAAKEQNISIL
                     SYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIKSLRGKGKSIEINK
                     LNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMYAIQKVPAMRKYSG
                     HISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4588 a         4646 c         4993 g         4585 t
ORIGIN      
        1 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgtccgtgg atgcgcacta
       61 tactccgctc gcgttttcat gcaacctttt ttttaccccg atcacggatt tagcatgatt
      121 ttgaacaaaa acaaaccact aaagcctatt ttttgtgcaa aatttgcgtc agggagaggc
      181 tgctcatttc attaaacgcg gcggtttgtt atcttcgttg cgccttattt tttaacctga
      241 agaagagaac aatgaaagga cgttgggtaa agtaccttct tatgggcacg gttgtggcaa
      301 tgcttgccgc ctgctcttcc aaaccaaccg atcgcggaca gcaatataaa gacgggaaat
      361 ttacccagcc tttctctctg gtgaaccagc cagatgccgt tggcgcgccg attaacgccg
      421 gtgattttgt cgagcaaatt aaccatatcc gtaattcgtc accgcgtctg tatggcaacc
      481 agagtaatgt ttataacgcg gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata
      541 tgcgccagtt cggcattgat gcctggcaga tggaaggtgc cgacaactat ggtaacgtgc
      601 agtttacagg ctattacacg ccggtaattc aggcgcgcca tacccgccag ggcgagttcc
      661 agtatcctat ttaccgtatg ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct
      721 acgccggggc attgggcgat aaatacatcc tcgcttacag caactctctg atggataact
      781 tcattatgga tgtgcagggt agtgggtata tcgactttgg tgatggcagt ccgcttaact
      841 ttttcagcta tgcagggaaa aacggtcatg cctatcgcag cattggtaag gtgctgatcg
      901 accgtggcga agtgaaaaaa gaagatatgt cgatgcaggc gattcgtcat tggggcgaaa
      961 cacacagtga agccgaggtt cgcgagctgc tggaacagaa cccgtctttc gtcttcttta
     1021 aaccgcaatc ttttgcaccg gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt
     1081 ctgttgcctc cgatcgttca attattccgc caggtactac cttgctggca gaagtgccgc
     1141 tgctggataa taacggcaaa tttaacggtc agtacgaatt gcgtctgatg gtggcgttgg
     1201 atgtcggggg cgcaatcaaa ggccaacact tcgatatcta tcaagggatc gggccggaag
     1261 ccggacaccg cgcaggttgg tacaaccact atggacgggt ttgggtgctg aaaacggctc
     1321 cgggcgcagg taacgtcttt agcggctgat gtgatattct gtatgccgtt tttacggata
     1381 tgacagggga ggggtaacct cacatcctga ttattgacta agtgcgcgtt gttcataccg
     1441 gatgcggcgt gaacgcctta tccggcctac aaatcatgca aatccaaaat attgcaggag
     1501 ttatgtaggc ctgataagcg tagcgcatca ggcatttatg cgtttagggt gagggataac
     1561 ctcgcccttt ttaattctga ggtgctatgt ctgtggtaat tagtgatgca tggcgtcagc
     1621 gttttggtgg cacagcgcgt ctgtatggtg aaaaagcgtt gcaactgttt gctgacgcgc
     1681 atatttgtgt ggttggtatc ggtggtgtcg gttcctgggc ggcggaagcg ctggcgcgca
     1741 cggggattgg cgcaatcacg cttatcgata tggatgatgt gtgcgtcacc aataccaatc
     1801 ggcaaattca cgctctgcgc gataacgtcg ggctggcaaa agcggaagtc atggcggagc
     1861 gcattcgcca gattaacccg gagtgccgtg taacggtggt ggatgatttc gtgacgccgg
     1921 ataacgtagc gcaatatatg agtgccggtt attcgtacgt gattgatgcc attgatagtg
     1981 tacggcccaa agcggcgctg attgcttatt gtcggcgcaa taaaatcccg ctggtcacga
     2041 ccggtggcgc gggtgggcag attgacccga cgcagattca ggttaccgat ctggcgaaaa
     2101 cgattcagga tccgctggcg gcgaagttgc gcgagcgcct gaaaagcgat tttggcgtag
     2161 tgaaaaacag taaaggtaag ctcggcgtgg attgcgtgtt ttctactgaa gcgctggtgt
     2221 acccgcagtc agacggtacg gtgtgtgcga tgaaagccac ggcagaaggg ccgaagcgga
     2281 tggattgtgc atctggattt ggcgcggcaa cgatggtgac cgccaccttt gggtttgttg
     2341 cggtttctca tgcgctgaag aagatgatgg cgaaagcggc gcgtcagggt taaacaatca
     2401 ggtcggatga gacgcgaacg tcttatccga cccggttctg tgcttaaacc tgcttcgtcg
     2461 cagcgataat cgcctcgctt aacgcattta acccctggct gcgtgaggcg ctaagctgcg
     2521 cacgtaatcc cagctcatca aacaatgcca gcggcgactg tgcctgtaat tcggcggcgg
     2581 ttttcccctc aacggcagtc aacaacaccg ccaacaggcc gcgcacaatg cgcccttcgc
     2641 tgttgccaaa gaagtgcatt ttgccgtttt cagccacggt atatcccagc cagacgcggt
     2701 tttcgcatcc ggcaatctct ttagcctgcg cttttaactc gtctggcaaa gccggaagct
     2761 gtttccccag catgatcaac tggcgatatt tatcttccca ttgcgacaac ggtgcgaagg
     2821 tattgcgtaa cgtttctgcg gttacggttg tgccgaacgg atgtccggcg aattgcgggt
     2881 ttgtcattaa tccaccaata attccagcgc gcggtcaacg gcattcacca gcgcatccac
     2941 atcactcttt gtattatatg gcgcaaatga ggcgcgcagt gtgccggtta cgcctaattc
     3001 tgccagtagc ggctgagcgc aatgttgccc ggcccgcaag gcaataccgt actccgccag
     3061 tagcgtcacc atatcgctgt gatgaacgcc agcaaaatca aaggccagca ggctggaatc
     3121 ctggcagcgg aatgaacgaa agcctggacg tttcgccagc gcttcttctg caagcgttgc
     3181 taagctacgg ctccagcttt cggcctggtt gatatcgtaa tctgccagcc attccagcgc
     3241 cgcgcttaat cctatgacgc cagcgacatt tggcgttcct gcttccagtt tccacggcgc
     3301 agattgagtg gtgaaaccgt caaaactcac ttcgtgaatc attttgccgc cacccagcca
     3361 gggcgacatc gcttccagca gttctgattt gccatacagc acgccgatac ccgtcgggcc
     3421 atacagtttg tgaccagaga aagcatagaa atcgatatcc agttgctgaa catccgcggg
     3481 gaaatgcacc gtcccctgag caccatcaac catcaccacc atcccggctg aatgagcaaa
     3541 ggtaatcgct cgcgccagat ccgggcaacc gccagtcacg ttcgacatct gacccaacgc
     3601 cagaatccga ctacggggag taatcagctc tggcaacaaa tcgacatccg gcagtcgctg
     3661 cgcattaagc ggcaatttca ccactttggc tctagtttgt tgggcgacca tcagccaggg
     3721 gacgaggtta gcgtggtgtt ctgccacgct gacaataatc tcatcgcccg gttgcagacg
     3781 cggacgcgca tagcattgtg ccaccatgtt gatggattca gtggtgccgc gcgtccagac
     3841 gatagtttta tcatccggtg cattcagtaa ttgcgccact ttctctcgtg cagcttcata
     3901 acgcgcggtc aggcgttggg cttcggcaaa ctggctgcga tggacgtttc cggcgctcag
     3961 gctgtaaaac tgtcgagtgg cttcaaccac ggcttcaggt ttaagcgcgg tcgcggcgct
     4021 gtcgagatag acgcccgcat cctgtagtgc gggaaactgg gcgcgaaact gcgcgggatt
     4081 aaaaacgttc atggtactcc tcggcttgat gcaccgatcg tgacgcaaaa ttcactttga
     4141 tacaaggaga gtgctaacca tcagaattat ctggatatcc tggcgaagcc gcgtaagtaa
     4201 catatgctga atagtgttag gcgttaattt tagcctgtta atcaatacta ttaagattta
     4261 ttaatcgttt taaggagaag atgatgaaaa agactgccgc aattatttct gcctgtatgc
     4321 tgacttttgc cctgagcgcc tgttccggtt cgaactatgt gatgcacacc aatgacggac
     4381 gtaccatcgt ctctgacggc aaaccacaga ctgataacga tatcggtatg atttcgtata
     4441 aagacgctaa tggcaacaaa cagcagatca accgtactga cgtgaaagag atggtcgaac
     4501 tggaacagta attcgcgatc gcaaggtaaa aaaaagcacc gcaattaggc ggtgctacat
     4561 taatcactat ggacagacag ggtaaatgta caggaagtga aaaaaggtag ctttgctacc
     4621 atggtctgaa tcgcagacca attgcaaaca caacaacaca acatcacaac cgtaagccaa
     4681 aagttcacca gaatacgcat tccgataaaa cttttcgttc cggctcagga agtgccgcca
     4741 ctataggtat ttgctggtag aagctcaacg gacaatttat aatggctcag attaaaaaaa
     4801 ctaataggtt acacagtgtg atctaattgt taaattcatt tgacatcaaa gtttaaaagc
     4861 catgtctaaa cgattaccac cgctaaatgc cttacgagtt tttgatgccg cagcacgcca
     4921 tttaagtttt actcgcgcag cagaagagct ttttgtgacc caggccgcag taagtcatca
     4981 aatcaagtct cttgaggatt ttttggggct aaaactgttc cgccgccgta atcgttcact
     5041 cctgctgacc gaggaagggc aaagctattt cctcgatatc aaagagatat tttcgcaatt
     5101 aaccgaagcg acgcgtaaac tccaggcccg tagcgccaag ggggcgttga cggtcagttt
     5161 actccccagt ttcgccattc attggttggt tccgcgactt tccagcttta attcagctta
     5221 tccgggaatt gacgttcgaa tccaggcggt tgatcgtcag gaagataagc tggcggatga
     5281 tgttgatgtg gcgatatttt atggtcgggg caactggccg gggctacggg tggaaaaact
     5341 gtacgccgaa tatttattgc cggtgtgttc gccgctactg ctgacaggcg aaaaaccctt
     5401 gaagaccccg gaagatctgg ctaaacatac gttattacat gatgcgtcac gccgtgactg
     5461 gcagacatat acccgacagt tggggtttaa tcatatcaac gttcagcaag ggccaatttt
     5521 tagtcatagc gccatggtgc tgcaagcggc tattcacggg cagggagtgg cgctggcaaa
     5581 taacgtgatg gcgcaatctg aaatcgaggc cggacgtctt gtttgcccgt ttaatgatgt
     5641 tctggtcagt aaaaatgctt tttatctggt ttgtcatgac agccaggcag aactgggtaa
     5701 aatagccgcc tttcgccaat ggatcctggc gaaagccgct gctgaacaag aaaaattccg
     5761 ctttcgttat gaacaataat ttacgtaggg tacgaccatg accagccgtt ttatgctgat
     5821 tttcgccgcc attagcggct tcatttttgt ggcactgggc gcttttggtg cgcatgtgtt
     5881 aagtaaaacc atgggggccg ttgagatggg ctggatccaa accggtctcg aataccaggc
     5941 gtttcatacg ctggcgatct taggtctggc ggtggcaatg cagcgtcgca tcagtatctg
     6001 gttttactgg agtagcgttt tcctcgcgtt aggcacggtg ctgttcagcg gcagccttta
     6061 ttgcctggcg ctgtcccatc tgcgtttgtg ggcgtttgtc acaccggttg gcggcgtgag
     6121 cttccttgcg ggctgggcgt taatgttagt tggtgctatc cgtttaaagc gcaagggcgt
     6181 aagtcatgaa taaggttgta ttgctgtgcc gtccgggctt tgaaaaagag tgcgccgcag
     6241 aaattaccga taaagccggc cagcgggaaa ttttcggttt tgcccgcgtg aaagagaatg
     6301 cgggttatgt catttatgaa tgttatcaac ctgatgatgg cgataagtta atccgtgagc
     6361 tgccgttcag ttcattaatt tttgcccgcc agtggtttgt ggtgggggaa ctcctgcagc
     6421 atttgccgcc agaagatcgt attaccccca ttgtcggcat gttacagggt gtagtagaga
     6481 agggcggtga actgcgtgtt gaagttgccg ataccaacga aagcaaagag ttgctgaaat
     6541 tctgccgtaa gttcactgtt ccgctacgcg ctgccttgcg cgatgctggg gtgctggcga
     6601 actatgaaac gccgaagcgt ccggttgtgc atgtattctt tattgcacca ggatgctgct
     6661 ataccggtta ctcatacagc aacaataatt cgccgttcta tatgggcatt ccgcgcctga
     6721 aatttccggc agaggcgccg agtcgttcca cgctcaaact ggaagaggca tttcatgtgt
     6781 ttattcctgc ggatgagtgg gatgaacgcc tggcgaacgg gatgtgggcg gtggatttag
     6841 gcgcttgccc tggcggctgg acctaccaac tggtgaagcg caacatgtgg gtttattccg
     6901 tcgacaacgg cccgatggcg caaagtctga tggataccgg gcaggtgacg tggctgcggg
     6961 aagacggttt caaattccgt ccgacacgca gcaatatctc ctggatggta tgcgatatgg
     7021 ttgaaaaacc ggctaaagtt gcggcattga tggcgcagtg gctggttaat ggctggtgcc
     7081 gtgaaaccat tttcaatctc aaactgccga tgaaaaaacg ctacgaagaa gtatcacaca
     7141 atctggcgta tattcaggca cagcttgatg aacatggcat aaatgctcag attcaggcgc
     7201 ggcagttgta tcacgatcgc gaagaagtga cggtgcacgt ccgccgcatc tgggctgcgg
     7261 tgggtggtcg tcgcgacgag cgataacagg caaggccggg gcgtcgtctc cggcctgcta
     7321 ccctttccgt caggctgtta ccaaagaagt tgcaacctgg cgctcatcag aaataatgtg
     7381 cgttacctca tccagatgcc cgatgcgggc ttcccctgaa cgattaaatt tacttttatc
     7441 aatcaataac aacgattgcg cggcacgttt taatagcatc gatttgtaat cagcgttgat
     7501 cgcattggag tcccacagtg cgccgctgct atcgatccct tcacaagaaa aaataaacag
     7561 atcgatttcc agcgatttaa gttgggaaat cagcgaggga ttgacgtaac agccatattt
     7621 gcgctcaagt gtgccgccgg aactgatcag ttgaatgcgt tcgcgtttac cgagttcatg
     7681 gcaaatcgga tggctattgg tgaagacctg aatgttgatg tcaggcaact ggcgtgccag
     7741 ataccagcaa gttgaactgg cgtctaaggc tatcaccatc ccttcttcaa tccacgcgag
     7801 cgcctcgcgc gcgatatctg ctttatgcgc ataatggctt ttcagcctga tgtgaaaggg
     7861 atcgccactg tcttgatttt gacggtggat atatttagcg cgtccatgat tgcgcagaat
     7921 tttaccctgc gtctgtaatt cattgagatc gcgacgaatg gtttctttac tgacctttag
     7981 ctgttcagag agagcttccg tggtcaggct ggtatggttc agcagcaggt cgactatcgc
     8041 ttgctggcgt gccgctttca tatttttcac cccatcattc gataaccgcc cgcgtcatgc
     8101 gggcgtaccg gtatgagatt acggtgttac cccttttttt aaaagaatat tcccgtactt
     8161 cgctcgttcg cctgtgataa cgatcgcaaa ggctttttgc gcccgttcat aaaacgcaaa
     8221 acgattgatg cggatgatgt caggacacgg ggcttgtagt gaaagcgcat tacggtaacg
     8281 tcgttctact tcaggatcga gagtgtcacc ctctaccgcc gccatcatca ccagcggcgg
     8341 ggcataactg tccagctcaa ataacgggat aatcgcctgg agcaagtcgc tcaccaacag
     8401 gccatcagcg cggatcacct gcggttccat cgaatgggcg ggaaagtgag catcggaaaa
     8461 aataatttca tctccatgtc ccatctctgc cagcactttc aatagttcgg gagaaattaa
     8521 cggcgaaatt gttttcagca tttcacactt cctctataaa ttcaggttca gtttgcgggt
     8581 agaaataacg gtactgataa tgaatctgtg cacgggcttc ttccgggctg ttaaattccc
     8641 ctacgccata ccaaccgaac agcgcagctc ctgcgacggt cgtttcggca tcgtcgagaa
     8701 cttttaccgg aatatcaagc atattggctt taatctgatt ccacaatgtg ttgcgacttc
     8761 ctccaccgac taacaataat tcataggctt taaagtgccc gattttttcc agcatctgta
     8821 gattgcgctg taattgcgca gttaaccctt ccagcgccgc gcgatagaaa tgcccccgcg
     8881 tggtattaag cgtcactcct tgccagccag cgttctgaca cgacaataaa tcacactgca
     8941 ttttcacacc atccgcgcca ggtgcgatca gacgagcttc ttcaatcaac atttgccagg
     9001 gtgtttcagc cgtccagaac agttttctca cccattccag cacgccggat gccagccatt
     9061 gcatacctgg gttatacaac catgcctggc tatctagttc gcaggtggaa ccggcgtact
     9121 gacttaacag cgaagtatca acctaggcgc tgcgaaccat taaaatttcc catgtaccgg
     9181 aggagagcac gggttcattt tgttcggcac cagcgccaaa aagggcgaac tgggtatcgt
     9241 gacctgcgga aatcaccggt atgccaacgg gtaagccgag cattgctgcg gcgttgttct
     9301 gtagcgtacc aatctgttca cccgcttcca ccagacgagg gaagagtcgg cgtggaatac
     9361 cggtggcttg taaaatttgc ggactgaaat cgcgttgctg gatatccagc atctggctgg
     9421 ttccggccat cgtgatatca gtagtgaatt cgctggttaa acggtggtta atcagcgacg
     9481 aaatgaagag ccaggcgtgc gcgcgttcca gcagttgtgg atgattttct ttcaaccaca
     9541 ccaacttata taacgtattg aaactaaagg ctccgacgcc agaaatagcc tgcaaccgct
     9601 gtgcggagat taaccgttca atattgtcca taaccgctgc tgttcgcgga catttccagc
     9661 taatgatcgg atagagcaga ttgccttgct tatctaccag agcgccatcc acactaaagg
     9721 tggtgacggc gataccgcgg atgtggcatt cagtcagttc actattgatt tgccgacagc
     9781 aatcagcaaa gcgttgcaaa atggcagtct aaagaccact ggtgccaggt gttgttttcc
     9841 atcgcgatat cgctggcatt aggcgttgag gcgcgggcaa caatttttcc ctgccgatta
     9901 accgcgatgg ccctgacatt ggtcgcgcca cagtcgagta ccaggataac ttcttgtttc
     9961 ataatcgctc ctgcaatata gccggataac attgtttatc cggctaacca ctcttgcttg
    10021 ttcaggcccg gtagccggag cgaccgggca tcacatcagg gtgtaatgta ttaacgcttg
    10081 tacaacggac cgtagttctg gcaagcgcgg taatcctggc cttcaatatc catgccgtgc
    10141 gcagcccagg cagaaggacg atacactttg gtctcttcaa cgttgtgcat acataccggg
    10201 atacgcagca tggaggcaag ggtgataaag tctgcgccaa cgtggccgat ggtcagaact
    10261 ccgtggttgg caccccagtt cgccattacc gaatacacat ccgtaaacgg ccctttacca
    10321 gtgaggcgcg gtgcaaacca ggtggttggc caggttgagt tggtgcgttt gttgaggatg
    10381 tcatgcacat ccttcggcaa ttccacgctc cagccttccg cgatttgcag taccggtccc
    10441 aggcctttga tgatgttgac acgagtcatg gtgaacggga cgccgccttc ggtaaggaag
    10501 cgggaagagt aaccgccgcc acggaagtat tcgtggatcg ccgggcacca ttcggtagcg
    10561 gcgaggcaag cgtcagcctc ttgctgagag atttcccagt gcggcttcat cgtcgggtta
    10621 ccttcgctgt cgcgttgttt acaggaaccg tccagcgcag cagaacctga gttgatcaaa
    10681 tggatgatgc cgtgttctgc cagtccgtcc agtttatgcc ccgttacacg ctcaattgct
    10741 tctggtgacc agtaggtacg cacatcggca aatacctgag cggtgccggt gagctggtga
    10801 cccattagca ttgccacgcc gttaagactg tcgttttcgg tcgccacgac aaagggttcg
    10861 cgcacgccat tccagtcaaa tgaactgttg aggatcgctt cggcggtgtc accattggga
    10921 tattgatcgg tccagtgacg ttgcccctgg aagcccgcag cgatggcgtt gtagccaagt
    10981 gattcttcca cgcgaccaat atcggccagt ttgctgttgc cttgcatcat gtcgcggata
    11041 cacatcgcca tcagtaaact ttcgcgcaga actgcgcggc tttgctcggc attgcgttga
    11101 tactgtttgt tattttcatc ttcgccatag cggaagtttt tatcagccca ggccagtgcc
    11161 atttccagtt cggcttcgtc gtaaatcttc tgatcgatac ggcgacgcag ttcggtcata
    11221 tccaccgcct ggactttcat tcccagccag gattcaaaga agttgtgatc aacaatagaa
    11281 ccggcgatac ccatcgaaac gccgcccagc gacagatagc ttttaccttt catgctggcg
    11341 acggccaaac cggcgcgggc aaagcgcagc agtttttctt caacatcggc aggaatcgat
    11401 gtgtcatcgg catcctgaac gtcatgaccg taaatggaga atgccgggat gcctttctgg
    11461 ctgtgagctg ccagagccgc agccaggtaa acagcgccgg ggcgttcagt gccgttaaag
    11521 ccccaaatgg ctttcgggcg ggttggatcc atgtcgatgg tttcactgcc atagcaccag
    11581 caaggcgtta ccgtaatggt gaggcctaca ttctggctgc tgaatttttc ttcgcaagca
    11641 gaggcttcag ccatacccgc gatacaggta tcggaaatga cacactcgac ggcagctccg
    11701 caggcatggc gcagtttctc ggtcagcagt gcggccgtag ctttcgccat attcattgtt
    11761 tgttcttcaa gcgactcacg aacacccatg cgacgaccgt caataaccgg gcggatacca
    11821 attttcggta agctgatttt tttcattaca gattcctcac tttattcgga aaatatgttc
    11881 agttagttgc cgtttgagaa cggaaacggg caaagataaa gatgaccgcg aagcagagtg
    11941 cggggatcag ttcagcagtg gggatgttgc ccgccgcgtc actgacaaaa cccatgaccg
    12001 gagtgacaat accgccgcca ataatggtca taacgatgaa ggacgaaccg tatttggtgt
    12061 cctggccgag attcttaatg cccagcgaga agattgttgg gtactgaatc gacataaagg
    12121 cgctgcataa agtcagggct attaagccca catgaccgcc agcgaaggct gagatcaggc
    12181 acagtgccat agcgattaat gcgtaggcgg ccaggacttt gtgtggtgcg aagcgactga
    12241 tgagccaggt accggtgaaa cgaccaataa agaagcacac catggttccg gttaaatagt
    12301 tagcggcaaa gcctgcagtc atacctagaa tttcttctac agcgtagcga atcaaatagc
    12361 tccagcaggc cgtttgtgcg ccgacatagc agaattgcgc taataccgcc cagcgccagt
    12421 gacgaatacg cgccagacga gaaagcgatg cggagaacga tccttgtttg gcgtcactgt
    12481 gattatcact ctgcaatgcc gggaatttcg tcagcatgat cagcagggcg accagtaaca
    12541 cgatagccac gatgatcata taaggtgtct gtaccgataa taccaggctg tgtttatacg
    12601 cactcaattg ctctggagac attttatcga gaacgtcttg cgattgatgt ggcacgttag
    12661 acaaaataag actttgccca aagacaaccg cgataattgc gccaaacgag ttaaatgttt
    12721 gcgcaagatt taagcggaag tgactactgc tttccggccc taataccgta acaaaagggt
    12781 ttgcggcagt ttccagacaa cctaatccgg ctgcaataat aaataggcca actaaaaata
    12841 aggtgtagtt cattatttct gcagcgggcc agaataatgc agcgcccaag gcatataaaa
    12901 ataatccggt aataatccct gctttataac tgagtttttt catcaatatc ccagcaggga
    12961 ttgggataat gaaataacca aagtaaaagg ccgattggat caggccagcc tggaaatttg
    13021 tcagcgtaaa agcctgctgg aattgaggta ataaaatgtc gttaaggtta ttggctaccg
    13081 cccaaagaaa aaacagtgag cacagcagcg cgaatggaat aatgtaactt ctgctttgcc
    13141 ctgcatcttt atctaccgca cggtaactct gcgtttgtat tgatgtgttt cccatagcat
    13201 cctcttaggt tcagaagctt aagcacgtaa tcggttatgt tttcaaaagt gcaaaaccag
    13261 cgagttcggg cttcatatcg gtgaatattt gcctgtctga aatgaactca tggattcatt
    13321 ttcaaataaa aaacgctctg taatatgacg gcggtcacac ttattagctg gaaggtgact
    13381 ttatgtgact accatcactt taataatcga aatttcatgc ccgtttcaga aaataaatca
    13441 taaaaaacac aaaccagatg accgtattaa ttgtttgtgc cgaaaacggg catttctggc
    13501 gaggggaaat ggaaaaatga ccgttttgat aaatattttt aagagcggtc atttttaacg
    13561 gggttataaa aagagaactt gcagttattt actgtgatta gttgagctag gtcacaaaat
    13621 ataagcttgt gaatttcttt cttgttaatc aggaagaaga tggtgggtaa ttaaacggct
    13681 aattcaatag tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct
    13741 aatggaacga aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct
    13801 gggactgaac caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat
    13861 tacgcctaca ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg
    13921 caacggtaaa catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcagc
    13981 ctatcaaagc agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc
    14041 agtttccatt cttaaccgat cgatccccgc tattcactac atgattgcgg cggctggcgg
    14101 taattctatt ccttgcgcgc cttatgcgac ctttggaaca cgcgaacttt ctgaacatgt
    14161 tgcgctggct ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg
    14221 tgaggtgaat ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact
    14281 ttacctgacg accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc
    14341 cgtagtgctg gagaaattca aaacctatgg gttacggatt gaagagtaat ttcgtaaagc
    14401 aacaaggaga aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg
    14461 gggtgctgtt ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat
    14521 cgtcaccgat aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga
    14581 tgctgcaggg ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt
    14641 cgtcaaagaa gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg
    14701 tggtggttct ccacaggata cttgtaaagc aattggcatt atcagcaaca acccggagtt
    14761 tgccgatgtg cgtagcctgg aagggctttc cccgaccaat aaacccagtg taccgattct
    14821 ggcaatcccc accacagcag gtactgcggc agaagtgacc attaactacg tgatcactga
    14881 cgaagaaaaa cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt
    14941 tattgatgct gacatgatgg atggtatgcc tccggcgctg aaagccgcga cgggtgttga
    15001 tgcgctcact catgctattg aggggtatat tacccgtggc gcgtgggcac taaccgatgc
    15061 actgcacatt aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga
    15121 taaggatgcc ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa
    15181 tgttgggtta gggttggtgc atggtatggc gcatccactg ggcgcgtttt ataacactcc
    15241 acacggtgtt gcgaacgcca tcctgttacc gcatgtcatg cgctataacg ctgactttac
    15301 cggtgagaag taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct
    15361 ggaaggggcg cgtaatgccg ctgttgaagc ggtgtttgcc ctcaaccgtg atgtcggtat
    15421 tccgccacat ttgcgtgatg ttggtgtacg caaggaagac atttcggcac tggctcaggc
    15481 ggcactggat gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt
    15541 agagctttac cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgatttgcg
    15601 gcgtatccgg ctcgccgtta ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc
    15661 aaatcggttt gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct
    15721 aattttttgc gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc
    15781 tgaaactcga ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc
    15841 tttgaactgc tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca
    15901 aattctttat cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt
    15961 aatgtcggtg aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc
    16021 ccggcctgtg tcactttgac cgccagtgtg gcggctaagt catcggcttc gttgccgctg
    16081 gctgaccagc acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg
    16141 tgcaactctt ccggcattgg cggtcgatcc gctttgtaat ctggtaaacg ctgatgacgc
    16201 cagccgctac tgcggttttc atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc
    16261 ataatgagct gatcgagcgc atgctggcag gtttcgacac agggcgaacc ctgaacggca
    16321 tgaatgcgac ggataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc
    16381 cccttaaaga tcaggcgata agggtaacat tcgatgccgg aacaggctat ggctggttga
    16441 gaaaacagga agaggcttcg caaaacgagg cctctgggag agattaatcg caggcaacga
    16501 tcttcatcgc caggccgccg cgagaggttt cgcggtactt ggcgttcatg tctttacctg
    16561 tttcgtacat ggtttcgata actttatcga ggcagacgcg cggttcgctg gtacggcgca
    16621 gtgccatacg cgcggcgttc accgctttca ctgctgcaat ggcgttacgc tcaatgcacg
    16681 gtacctgaac ctgccccgcg accgggtcac aagtcagacc gaggttgtgc tccatcgcga
    16741 tttccgctgc gatgcaaacc tgcgccgggc ttgcgcctaa cagttctgcc agaccagctg
    16801 ccgccataga gcacgccacg ccaacttcac cctggcaacc cacttcggca ccagaaatcg
    16861 acgcgttcat cttataaaga gaaccaatgg cgctggctac cagcaggtaa cgagccagtg
    16921 agttagcgtt cacttcgcgg ataaacttat cgtagtacgc cagcacagcc gggataatcc
    16981 cgcacgcacc gttagtcggc gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca
    17041 ctgccagtgc aaacatgttg atccagtcaa caaccgccat cgggtcagtg gtggttttat
    17101 cctggctgac cagcatccgg cgtagtgccg cagcacggcg tggaacgcgc agtttgccag
    17161 gcaacacgcc ttcggtggaa ataccgcgct caataccgcc acgcatgact tcccagacgt
    17221 tcgccaggtg ctgttccagc tcttctttac tgtgcagcgc cagctcgttt ttcatcatca
    17281 ggccagagag tgacagcccg gtttcctgac aatgtttttg cagatcggct gctgaactgt
    17341 acggataagg aacttcaacc ggtgcgctat cctgctggcc aaaatgctct tcatcaacga
    17401 taaagccacc gccaatagag tagtaagtct ggctgtaaac gactttatcg cccgccagcg
    17461 cggtaatgcg cataccgttt tcatgcagag aaaggttgtc tgcatggaag ttcatgcact
    17521 gatcaaccgg gaactccact tcatgctgac cgtttgccag catcaggcga ccatgagtat
    17581 tcacatcctg aataaaaccg gggatggaat cgatatccac ggtatccggc aggttacccg
    17641 ccaggcccat aataatggcg atatctgtgt ggtggccttt accagtcaga gagagcgagc
    17701 cgtacacgtc aaccaccacg cgggtcacgt ctttaagcag gttacgggca atcagatcgt
    17761 cggtaaattg tttacccgct ttcattggtc caacggtatg agaactggaa gggccaatgc
    17821 cgattttgaa aatatcgaat acgctaatca taggaaatac atcgcgttaa aacggaggaa
    17881 gcgccgcccg aaagcggcgc gaaaggactt agctgaacag agagtagaag attgcggaga
    17941 ttgcaatcag acccatcacg acaacgaata cgttgctgat gtgaccgctg tacttacgca
    18001 ttgccggtac tttctgaatt gcgtacatcg gcatcaggaa caggatcatc gcgatgattg
    18061 ggccgcccag ggtttcaatc atacccagga tgctcgggtt cagggtggca acaatccagg
    18121 tcgttaccag catgaacagc gcagtgatac ggttcagctt gttgatttcg atagacttac
    18181 ctttaccacg cagagattta atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt
    18241 ggccgaggaa ggatttggtg atagcgataa tcgcgataat cggagccatc cacgcgataa
    18301 ccggtgcgtt aaagtggtta gccaggtaag acagaatcga gatgttctgc tctttagccg
    18361 cagccaggtc tgccggagtc aggctcagta cgcagctgaa gacgaagaac attacggtca
    18421 gcaccatcat gatgtgtgcg aatgccagga tcttcgagca tttctgttct gccatatcgc
    18481 cgtactcttc acgcttcgca acggcgaaag aagagatgat cggagagtgg ttgaacgaga
    18541 acaccattac cggaattgcc agccacaggg tcatccacag accgtttccg gttgcagatg
    18601 cagtgtccag agacagcgtt tccagtgctg cgccgttcca ctgcgggatc aggtacagag
    18661 ccagcagcat cagtacgcca acaaacggga ataccagaat actcatcgct ttaacgatca
    18721 tctgctcacc aaagcgaacg atggtcatca tacccacgat caggatcagc gacagaatcg
    18781 cacgcggcgg tggcgtcata cccagctggt ga
//
LOCUS       DAJVPT010000080        18770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVPT010000080 DAJVPT010000000
VERSION     DAJVPT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(139..3654)
                     /locus_tag="OQM47_002740"
     CDS             complement(139..3654)
                     /locus_tag="OQM47_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1480833.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OQM47_002741"
     CDS             complement(4107..5369)
                     /locus_tag="OQM47_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1480834.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OQM47_002742"
     tRNA            complement(5567..5642)
                     /locus_tag="OQM47_002742"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OQM47_002743"
     CDS             complement(5748..6455)
                     /locus_tag="OQM47_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1480835.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OQM47_002744"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OQM47_002744"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1480836.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OQM47_002745"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OQM47_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1480837.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OQM47_002746"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OQM47_002746"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1480838.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OQM47_002747"
     CDS             complement(11639..11914)
                     /locus_tag="OQM47_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1480839.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OQM47_002748"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OQM47_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1480840.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OQM47_002749"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OQM47_002749"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1480841.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OQM47_002750"
     CDS             13874..14200
                     /locus_tag="OQM47_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1480842.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OQM47_002751"
     CDS             14384..15103
                     /locus_tag="OQM47_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1480843.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OQM47_002752"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OQM47_002752"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1480844.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OQM47_002753"
     CDS             16442..17404
                     /locus_tag="OQM47_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1480845.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18770)
                     /locus_tag="OQM47_002754"
     CDS             complement(17505..>18770)
                     /locus_tag="OQM47_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1480846.1"
                     /translation="FFVLVFIGVPISFSIGIATVASMLLMFPWDIAAITVSQRLANGL
                     DNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISG
                     SAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVS
                     VASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLLVF
                     IVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLI
                     GFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFT
                     PIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLL
                     FYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4886 a         4576 c         4639 g         4669 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa
//
LOCUS       DAJVPT010000081        18495 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVPT010000081 DAJVPT010000000
VERSION     DAJVPT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..158
                     /locus_tag="OQM47_002755"
     CDS             <1..158
                     /locus_tag="OQM47_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1480847.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
     gene            184..1417
                     /locus_tag="OQM47_002756"
                     /pseudo
     CDS             184..1417
                     /locus_tag="OQM47_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1567..1998
                     /gene="ndk"
                     /locus_tag="OQM47_002757"
     CDS             1567..1998
                     /gene="ndk"
                     /locus_tag="OQM47_002757"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1480848.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2148..3302
                     /gene="trmG/rlmN"
                     /locus_tag="OQM47_002758"
     CDS             2148..3302
                     /gene="trmG/rlmN"
                     /locus_tag="OQM47_002758"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1480849.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3587..4600
                     /gene="rodZ"
                     /locus_tag="OQM47_002759"
     CDS             3587..4600
                     /gene="rodZ"
                     /locus_tag="OQM47_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1480850.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4627..5754
                     /gene="ispG"
                     /locus_tag="OQM47_002760"
                     /gene_synonym="gcpE"
     CDS             4627..5754
                     /gene="ispG"
                     /locus_tag="OQM47_002760"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1480851.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5857..7131
                     /gene="hisS"
                     /locus_tag="OQM47_002761"
     CDS             5857..7131
                     /gene="hisS"
                     /locus_tag="OQM47_002761"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1480852.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7149..7769
                     /gene="yfgM"
                     /locus_tag="OQM47_002762"
     CDS             7149..7769
                     /gene="yfgM"
                     /locus_tag="OQM47_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1480853.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7780..8958
                     /gene="bamB"
                     /locus_tag="OQM47_002763"
     CDS             7780..8958
                     /gene="bamB"
                     /locus_tag="OQM47_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1480854.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9076..10548
                     /gene="der"
                     /locus_tag="OQM47_002764"
     CDS             9076..10548
                     /gene="der"
                     /locus_tag="OQM47_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1480855.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10618..10833
                     /locus_tag="OQM47_002765"
     CDS             10618..10833
                     /locus_tag="OQM47_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1480856.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10830..12200)
                     /gene="xseA"
                     /locus_tag="OQM47_002766"
     CDS             complement(10830..12200)
                     /gene="xseA"
                     /locus_tag="OQM47_002766"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1480857.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12362..13828
                     /gene="guaB"
                     /locus_tag="OQM47_002767"
     CDS             12362..13828
                     /gene="guaB"
                     /locus_tag="OQM47_002767"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1480858.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13897..15474
                     /gene="guaA"
                     /locus_tag="OQM47_002768"
     CDS             13897..15474
                     /gene="guaA"
                     /locus_tag="OQM47_002768"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1480859.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15569..16108)
                     /locus_tag="OQM47_002769"
     CDS             complement(15569..16108)
                     /locus_tag="OQM47_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1480860.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16124..16642)
                     /locus_tag="OQM47_002770"
     CDS             complement(16124..16642)
                     /locus_tag="OQM47_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1480861.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16953..17144)
                     /locus_tag="OQM47_002771"
     CDS             complement(16953..17144)
                     /locus_tag="OQM47_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1480862.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17162..17314
                     /locus_tag="OQM47_002772"
     CDS             17162..17314
                     /locus_tag="OQM47_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480863.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17496..>18495
                     /gene="pdeF"
                     /locus_tag="OQM47_002773"
     CDS             17496..>18495
                     /gene="pdeF"
                     /locus_tag="OQM47_002773"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1480864.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVD"
BASE COUNT         4381 a         4499 c         5002 g         4613 t
ORIGIN      
        1 ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt cagggaaaac
       61 gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg ttcgccatga
      121 tgcgcgacgg gactttttat accccgcagg ggtcataaca tgcttgacaa cttaatagag
      181 gcacgttacc ttgaagcgct gatcaattat gaagatcgct ggttctggaa gcatccgggg
      241 gttaatccat tctcggtggc gcgcgcagca tggcaagatc tcacttcggg acgggttatt
      301 tccggtggca gcacgctcac tatgcaggtt gctcgtctgc ttgatcctca ccccaaaaca
      361 tttggtggca aaattctcca gctctggcgc gcgttgcaac tggagtggca tttgtctaag
      421 cgtgaaattc tgatcttgta tcttaaccgc gctccgtttg gcggtacgtt gcaggggatc
      481 ggtgcggcaa gttgggctta tctcggaaaa tcgcctgcga atttaagcta ttccgaggcg
      541 gcaatgctgg cggtgttacc gcaagtgccc agccgactgc gcccggatcg ttggccggag
      601 cgtgccgaag ccgcgcgtcg ccccactggc atggaaaacg ggcaccagct atggctatcg
      661 tgacgcctgg gcgattgggg ttaacgctcg ctatgtcatt gggatctgga ctggcagacc
      721 tgacggcacg cccgttgttg gtcagtttgg ctttgccagt gccgtaccat tgttgaatca
      781 ggtcaataat atcttactgt cgcgcagtgc gaatctgccg gaagacccgc gcccgaactc
      841 agtcactcgt ggcgttatct gctggccggg cggacagtct ttgccggaag gtgacggtaa
      901 ctgtcgccgc cgcctggcaa cctggctgct ggatggcagc cagccgccaa cgctgttact
      961 gtcagagcag gaagggatta atggcattcg cttccccatc tggctggatg acaaagggca
     1021 gcgcgtcgcc gctgactgcc cgcaggcgaa gcaagaaatg gtaaatgtct ggccgctacc
     1081 gctggaaccc tggctgcccg catcagaacg tcgcgctgtg cgcttaccac cggcctcgac
     1141 aatttgcccg ccatacggtc acgatgccca gctccctctg cagctgacag gcgttcgcga
     1201 tggcgcgatt attaaacgtt taccgggcgc agcggaagcg actttgccgt tgcaatcaag
     1261 tggaggggca ggtgaacgct ggtggtttct gaatggcgaa ccgttaactg aacgcgggcg
     1321 caacgtgacc ctgcatttga cggataaagg tgattatcaa ttgctggtga tggatgatgt
     1381 ggggcaaatc gcgacagtga aatttgtcat gcaatagtct gttttgtccc tgattgttgc
     1441 taaaactcat tttattttaa aaaaaatgtt accttcatca atagtcaacg gccctgttgc
     1501 tcattataat ccgcgccatc tcgtacgctg gtacagacaa caacagaaca atttacagag
     1561 gtaaaaatgg ctattgaacg tactttttcc atcatcaaac cgaacgcggt agcaaaaaac
     1621 gtcattggta atatctttgc gcgctttgaa gctgcagggt tcaaaattgt cggcaccaaa
     1681 atgctgcatc tgaccgttga acaggcacgt ggcttttatg ctgaacacga tggaaaaccg
     1741 ttctttgatg gtctggttga attcatgacc tctggcccga tcgtggtttc cgtgctggaa
     1801 ggtgaaaacg ccgttcagcg tcaccgcgat ctgctgggcg cgaccaatcc ggcaaacgca
     1861 ctggctggta ctctgcgcgc tgattacgct gacagcctga ccgaaaacgg tacccacggt
     1921 tctgattccg tcgaatctgc cgctcgcgaa atcgcttatt tcttcggcga aggcgaagtg
     1981 tgcccgcgca cccgttaata atttcgtaaa tgccgcgtgc aaacgtggca tccgtgcgcc
     2041 agaatttgta caatgcagcg cccccggacg agcagccgct caccggggcg tttctttttt
     2101 caaccctcca ggggccataa cgtgtaataa cgaggccgga atagcatatg tctgaacaat
     2161 tagtcacacc tgaaaacgtc accacgaaag atggaaaaat caacctgctg gatctcaacc
     2221 gtcagcagat gcgggagttt tttaaagatt taggtgaaaa aaccttccgc gccgatcagg
     2281 tgatgaagtg gatgtatcac tattgctgcg acaactttga tgagatgacc gacatcaaca
     2341 aagtgttgcg cggcaaactg aaagaggtgg cggaaatccg cgcaccggaa gtggttgaag
     2401 aacagcgttc atccgacggc accattaaat gggcgatcgc cgttggcgat cagcgcgtcg
     2461 aaacggtgta tatcccggaa gacgaccgtg ccacgctctg cgtctcttcg caggtggggt
     2521 gtgcgctgga gtgtaaattc tgttccaccg cccagcaggg ctttaaccgc aacctgcggg
     2581 tatcggaaat tattggccag gtgtggcgtg cggcgaaaat cgtcggcgca gcaaaagtca
     2641 ccggtcagcg cccaatcact aacgtcgtga tgatgggcat gggcgagccg ctgctcaacc
     2701 tgaacaacgt cgttccggcg atggaaatca tgcttgatga tttcggtttt ggcctgtcta
     2761 aacgtcgcgt gacgctttcc acttccggtg tagttccggc gctggataaa ctgggcgata
     2821 tgatcgacgt tgcactggca atttccctgc acgcgccgaa cgacgaaatt cgtgacgaaa
     2881 ttgtgccgat caacaaaaag tacaatatcg aaacgttcct tgctgcggtg cgccgttatc
     2941 tggagaaatc caacgccaat cagggccgag tcactattga atacgtgatg cttgaccacg
     3001 ttaacgacgg cactgaacac gcgcaccaac tggcggaact gctgaaagat acgccgtgta
     3061 agatcaacct gatcccgtgg aacccgttcc cggacgcgcc gtatggacgc agttcgaaca
     3121 gccgtatcga ccgtttctca aaagtgctga tgagctacgg tttcaccact attgtgcgta
     3181 aaacgcgtgg tgatgatatc gatgctgcct gtggtcagtt ggcgggcgat gttatcgacc
     3241 gtacgaaacg tactctgcgt aaacggatgc agggtgaagc catcgatatt aaagcggtct
     3301 gatagccatt acgccacggt tactttatgt accgtggcgt aatcttttat gaatgaagta
     3361 ttaagttgca attgtctggt caatattaac ggtgcgtttt tgctgacttt aaggcagtat
     3421 gtaacgatgc aacagtaagt tagcctcagt gaatgtgggc tttgtcacga gcacacagac
     3481 ggtcttatac tgtatgataa cggttaactt aacggatgtt tcgcggtgtg ggtgagcatg
     3541 atggttcacc ggcatctcaa ttctcattta aacgtacctg cagcgaatga atactgaagc
     3601 cacgcacgac caaaatgaag cacttactac cggcgctcgc ctgcgtaatg ctcgcgaaca
     3661 actaggactt agtcagcagg ccgttgccga gcgactttgc ctgaaggttt ccacggtacg
     3721 cgacattgaa gaagataagg cacccgccga tcttgcttca acattcctgc gcggatatat
     3781 ccgctcttat gcgcgtctgg tacatattcc agaagaagaa ctgctgccag ggctggaaaa
     3841 gcaggctcca cttcgggctg caaaagttgc gccaatgcag agtttttccc tcggtaaacg
     3901 ccgcaaaaaa cgcgacggct ggctaatgac cttcacctgg ctggtgttgt ttgtggttat
     3961 cggcctgagc ggtgcctggt ggtggcaaga ccacaaagct cagcaggaag agatcaccac
     4021 tatggccgat caatcttcgg cggaactgag cagtaatagc gagcaggggc agagtgttcc
     4081 gttaaatacg tcgacaacta cagacccggc tacaacgtcc acgccgccag cgtctgtgga
     4141 tactaccgca accaacacgc aaacacctgc cgtaactgcg ccagcaccag ctgttgatcc
     4201 gcaacagaat gcggttgttt cgccttcgca ggcaaatgtt gataccgccg cgaccccggt
     4261 accaacggca gcaacaacgc cagatggtgc tgcgccgttg ccaaccgatc aggctggcgt
     4321 gaccacgccg gcggctgatc cgaatgcgct ggtgatgaac tttactgccg attgctggct
     4381 ggaggtcact gatgctaccg gtaaaaaatt gtttagcggg atgcagcgta aagacggtaa
     4441 tttgaattta accggccagg caccgtataa actgaaaatt ggtgcgccag ccgcagtaca
     4501 gatccagtat caagggaaac ctgtcgatct gagtcgtttt atcagaacta accaggttgc
     4561 gcgtctgacc ctcaatgccg aacaatcacc ggcgcagtaa cagacgggta acgcgggaga
     4621 tttttcatgc ataaccaggc tccaattcaa cgtagaaaat caacacgtat ttacgttggg
     4681 aatgtgccga ttggcgatgg tgctcccatc gccgtacagt ccatgaccaa tacgcgtacg
     4741 acagatgtcg aagcaacggt caatcaaatc aaggcgctgg aacgcgttgg cgctgatatc
     4801 gtccgtgtct ccgtaccgac gatggacgcg gcagaagcgt tcaaactcat caaacagcgg
     4861 gttaacgtgc cgctggtggc tgacatccac ttcgactatc gcattgcgct gaaagtagcg
     4921 gaatacggcg tcgattgtct gcgtattaac cctggtaata tcggtaatga agagcgtatt
     4981 cgcatggtgg ttgactgtgc gcgcgataaa aacattccga tccgtattgg cgttaacgcc
     5041 ggatcgctgg aaaaagatct gcaagaaaag tatggcgaac cgacgccgca ggcgttgctg
     5101 gaatccgcca tgcgtcatgt tgatcatctc gatcgcctga acttcgatca gttcaaagtc
     5161 agcgtgaaag cgtctgacgt cttcctcgct gttgagtctt atcgtttgct ggcaaaacag
     5221 atcgatcagc cgctgcatct ggggatcacc gaagccggtg gtgcacgcag cggggcagta
     5281 aaatccgcca ttggtttagg tctgctgctg tcggaaggca tcggcgacac gctgcgtgta
     5341 tcgctggcgg ccgatccggt cgaagagatc aaagtcggtt tcgatatttt gaaatcgctg
     5401 cgtatccgtt cgcgagggat taacttcatc gcctgcccga cctgttcgcg tcaggaattt
     5461 gatgttatcg gtacggttaa cgcgctggag caacgcctgg aagatatcat cactccgatg
     5521 gacgtttcga ttatcggctg cgtggtgaat ggcccaggtg aggcgctggt ttctacactc
     5581 ggcgtcaccg gcggcaacaa gaaaagcggc ctctatgaag atggcgtgcg caaagaccgt
     5641 ctggacaaca acgatatgat cgaccagctg gaagcacgca ttcgtgcgaa agccagtcag
     5701 ctggacgaag cgcgtcgaat tgacgttcag caggttggaa aaataataac gtgatgggaa
     5761 gcgcctcgct tcccgtgtat gattgaaccc gcatggctcc cgaaacattg agggaagcgt
     5821 tgagggttca tttttatatt cagaaagaga ataaacgtgg caaaaaacat tcaagccatt
     5881 cgcggcatga acgattacct gcctggcgaa acggccatct ggcagcgcat tgaaggcaca
     5941 ctgaaaaacg tgctcggcag ctacggttac agtgaaatcc gcttgccgat tgtagagcag
     6001 accccgctat tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa agagatgtac
     6061 acctttgagg atcgcaatgg cgacagcctg actctgcgcc ctgaagggac ggcgggctgt
     6121 gtacgcgccg gcatcgagca tggtcttctg tacaatcagg aacagcgtct gtggtatatc
     6181 gggccgatgt tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt ccatcagttg
     6241 ggctgcgaag ttttcggtct gcaaggtccg gatatcgacg ctgaactgat tatgctcact
     6301 gcccgctggt ggcgtgcgct gggtatcttc gaacacgtaa ctcttgagct gaattctatc
     6361 ggttcgctgg aagcacgcgc caattaccgc gatgcgctgg tggcattcct tgagcagcat
     6421 aaagaaaagc tggacgaaga ctgcaaacgc cgcatgtaca ctaacccgct gcgcgtgctg
     6481 gattcaaaaa atccggaagt gcaggcgctt ctcaacgacg ctccggcatt aggtgattat
     6541 ctggacgagg aatctcgtga gcattttgcc ggtctgtgca aactgctgga gagcgcgggg
     6601 atcgcttaca ccgtaaacca gcgtctggtg cgtggtctgg attactataa ccgtaccgtt
     6661 ttcgagtggg tgactaacag tctcggctcc cagggcaccg tgtgtgcagg cggtcgttat
     6721 gacggtcttg tggaacaact gggcggtcgt gcaacaccgg ctgtcggttt tgcgatgggc
     6781 ctcgaacgtc ttgtattgtt agtacaggcc gttaatccgg aatttaaagc cgatcctgtt
     6841 gtcgatatat acctggtggc ttcaggtgct gatacacaat ctgcggctat ggcattagct
     6901 gagcgtctgc gtgatgaatt actgggcgtg aaattgatga ccaaccacgg cggcggcaac
     6961 tttaagaaac agtttgcccg tgctgataaa tggggtgccc gcgttgctgt ggtgctgggt
     7021 gagtctgaag tggctaacgg cacagcagta gtgaaggatt tgcgctctgg tgagcaaacg
     7081 gcagttgcgc aggatagcgt agccgcgcat ttgcgcacgt tactgggtta aggaaggaga
     7141 aggacagcgt ggaaatttac gagaacgaaa acgaccaggt agaagcggtt aaacgctttt
     7201 ttgctgaaaa tggcaaagca ctggctgttg gggggatttt gggcgttggc gcactgactg
     7261 gctggcgcta ctggaacagc catcaggttg attctgcacg ctccgcttct cttgcctatc
     7321 aaaatgcggt taccgcagtg agcgaaggca aaccggatag catcccggcg gcggaaaaat
     7381 ttgctgctga aaataaaaat acttatggtg cgctggcttc tttggaactt gcgcagcaat
     7441 ttgttgacaa aaatgaacta gagaaagctg ccgcccagtt acaacagggg ctggcagaca
     7501 cgagcgatga aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt caggtacagc
     7561 tcaagcaggc tgatgccgcg ctgaaaaccc ttgataccat caaaggtgaa gggtgggctg
     7621 ccattgttgc cgacttgcgt ggtgaagcat tgctgagcaa aggtgataag caaggtgcgc
     7681 gtagtgcatg ggaagcaggc gtgaaaagcg atgttactcc ggcactgagc gaaatgatgc
     7741 agatgaaaat taataatttg tccatctgag agggacccga tgcaattgcg taaattactg
     7801 ctgccaggac tgctttccgt taccctttta agcggctgtt cgctgtttaa cagcgaagaa
     7861 gatgtggtaa agatgtcccc attgccaacc gttgaaaacc agtttacgcc gaccacggcg
     7921 tggagcactt ccgttggtag cggcattggc aacttctatt ccaatcttca tccggcactg
     7981 gcggacaacg ttgtctatgc agcggaccgc gctggtttgg taaaagcgct gaatgcggat
     8041 gatggcaaag aaatctggtc tgtcagcctg gccgagaaag atggctggtt ctctaaagag
     8101 cctgcattac tttctggcgg tgtgaccgtg tctggtgggc atgtctacat tggcagcgaa
     8161 aaggcgcagg tttacgcgct gaataccagc gatggtactg tggcatggca aactaaagtc
     8221 gcgggtgaag cactttcgcg cccggtggtc agcgacggtc tggtgttaat ccacaccagt
     8281 aacggtcagt tacaagcgct gaacgaagct gacggcgctg tcaaatggac agttaacctc
     8341 gatatgcctt cgctctcttt gcgtggcgag tctgcgccgg caacggcttt tggtgcggcc
     8401 gtcgtgggtg gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg ccagatgatt
     8461 tggcagcagc gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct gagcgatgtt
     8521 gacacgactc ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa tggtaacctg
     8581 acggcgcttg atctgcgcag tggtcagatt atgtggaaac gcgaactggg ttcggtgaat
     8641 gatttcatcg tcgatggcaa tcgcatctat ctggtcgatc aaaatgaccg ggtgatggcg
     8701 ttgaccattg atggcggcgt tacgctgtgg gcacaaagcg atctgctgca tcgcctgctg
     8761 acttctccgg tgctgtataa tggcaacctg gtggtcggtg acagtgaagg ttatctgcac
     8821 tggattaacg tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag ttccggtttc
     8881 cagactgaac cggttgccgc tgacggcaaa ctgctgatcc aggcaaaaga cggaaccgtg
     8941 tactctatta cacgttaatc gtctctgtcg ttcactttga aaacggctcc tggacagggg
     9001 ccgttttcct gtttttaaca acgacgcgaa tatagcgtgc gttgtctgat gatttataaa
     9061 aatgaggctt taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa cgtaggaaaa
     9121 tccacgttat ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga tttcccgggt
     9181 ctgactcgtg accgtaagta cggtcgtgcg gaaattgaag gccgtgagtt tatctgtatt
     9241 gataccggcg ggattgatgg cacagaagac ggtgtagaaa cccgcatggc ggaacagtcg
     9301 ctgctggcga ttgaagaagc ggacgtcgta ctgtttatgg tggatgcgcg cgcgggcctg
     9361 atgccggcag atgaagcgat tgccaaacat ctgcgctccc gtgaaaaacc gaccttcctg
     9421 gtggtaaaca aaactgacgg tctggatccc gatcaggcag tggttgattt ctactcgctt
     9481 ggtttaggtg aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt aagtctgctg
     9541 gagcatgtgc tgctgccgtg gatggaagat ctcgcaccgc aagaggaagt cgacgaagac
     9601 gctgaatact gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga ggaagacgac
     9661 ttcgacccgc aaagtctgcc gatcaaactg gcgattgtgg gtcgtccgaa cgtaggtaag
     9721 tctacactca ctaaccgtat tcttggagaa gagcgcgttg ttgtttacga catgcctggc
     9781 acgacgcgtg acagcatcta catcccaatg gaacgcgatg gacgtgagta tgtgctcatt
     9841 gacaccgctg gcgtacgtaa acgcggcaaa atcaccgatg ctgtagagaa attctccgta
     9901 atcaaaacgt tgcaggccat tgaagacgcc aacgtggtga tgttagtgat tgatgcgcgc
     9961 gaaggtattt ccgatcagga tctctcgctg ctgggcttta ttctcaatag tgggcgctca
    10021 cttgtcattg tggtgaataa gtgggatggc ctgagccagg aagtgaaaga gcaggtgaaa
    10081 gaaacgctgg acttccgtct gggctttatc gattttgctc gtgtgcactt tatctctgcc
    10141 ttgcacggca gtggtgttgg taacttgttt gaatcagtac gtgaagcgta tgacagctcc
    10201 acccgtcgtg tggggacctc tatgctgacg cgcatcatga cgatggctgt tgaagatcac
    10261 caaccgccgc tggtacgcgg tcgtcgtgtg aagctgaaat atgcccacgc tggtggttat
    10321 aacccgccga ttgtggtgat tcacggtaat caggtgaaag acctgcctga ttcctacaag
    10381 cgctacttga tgaactactt ccgcaaatcg ctggacgtaa tgggatcgcc gattcgtatt
    10441 cagttcaaag aaggggaaaa cccgtatgcg aacaagcgta acaccctgac gccaacccag
    10501 atgcgtaaac gtaagcgtct gatgaagcac atcaagaaaa ataaataatt atccgtaagt
    10561 attttatctg ccattccctc tatgaatgta gagggaatgg ctaccggagg aattcatatg
    10621 gaactgcatt gtccaaaatg tcagcatgtg cttgatcagg ataacggcca tgcgcgttgc
    10681 ccatcctgcg gggaatttat tgaaatgaaa gctctttgtc cagactgcca tcaaccgttg
    10741 caggtgttaa aagcctgcgg tgcagtagat tacttctgtc agcacgggca tggcttgata
    10801 tcgaaaaaac gcgttgaatt cgtgctggct taatgaacct ttttacgcga tttttttacc
    10861 ggctggatgt tttttacttc actttctatc cagccgtctt ccagacgtgt ggttagcatt
    10921 tcacccgttt taacttgctt cacttttttc agtacattgc cgtcagtagc agtagtaacg
    10981 ctgtatccac gcgccagcgt tgacagtggg cttacggctt cgaggtgcgt tactgcatta
    11041 ccgaaacgtt cacgcgtggc gctaagctgt gcgcgcagga tttctgctaa acgatattcc
    11101 agttgctgaa tgcgcgtttg cgtgcgatga atcttcggct gtggattttg ctgattcagc
    11161 cgctgtgtta atcgctgctg ctgttgcccg gcacgcttaa gctgattttc cagcgcaaag
    11221 ctcatccgtt tttgcaggcg ttcaagcatg gtttgctggc gtgccagccg gagctgcgga
    11281 tgctgctgct gcaagcgatg atgaatctgc gtaaagcgac gtgtacggtt ggcgagataa
    11341 taatccatcg ccatctccag ccgttgacgg gccgattgca cctggcgcag taactcttgc
    11401 tgattacggc tcactacttc agcggcggca gacggcgttg gcgcacgcag atcggcaaca
    11461 aaatcggcaa tggtcacatc cgtctcatgc ccgacggcgc tgacgaccgg aatgcggctg
    11521 gcaaaaatcg cccgcgctac gcgttcgtcg ttaaaactcc ataaatcttc cagcgaaccg
    11581 ccgccgcgcc caacgatcaa cacgtcgcac tcattgcgct gattcgccag ttcaatggcg
    11641 cgaacaattt gccccggcgc gtcatcgccc tgaacggagg tggggtagat gatcaccggt
    11701 agagaaggat cgcgacgttt taacacatgc aaaatatcat gtagcgcagc accggttttt
    11761 gaggtgatca caccaacgca atgcgcaggg gagggaagtg gttttttgta ttgctgatcg
    11821 aacaaacatt cagcctgcaa cttcgctttg agctgttcgt acttctgttg cagcagccct
    11881 tcaccggccg gctgcatact ctcaacgatt atctggtagt cgccgcgcgg ctcgtagagc
    11941 gtaatattgg cgcgaactaa aacttgttgc ccatgctgcg gacggaaggt gacccggcgg
    12001 ttgctgttgc ggaacatcgc gcagcgtacc tgggcggtgt cgtctttgag tgtaaagtac
    12061 cagtgaccgg aagccggttg tgtgaaatta gagatttcgc cgctgatcca aacctgtccc
    12121 atctcatgct caagcagcag acgaaccgtt tgattcaggc gactaacggt aaaaattgca
    12181 ggggattgag aaggtaacat gtgagcgaga tcaaattcta aatcagcagg ttattcagtc
    12241 tatagtaacc cgcccttcgg ggatagcaag cattttttgc aaaaaggggt agatgcaatc
    12301 ggttacgctc tgtataatgc cgcggcaata tttattaacc actctggtcg agatattgcc
    12361 catgctacgt atcgctaaag aagctctgac gtttgacgac gttctcctcg ttcctgctca
    12421 ttctaccgtt ctgccgaata ctgctgacct cagcacccag ctgacgaaaa ctattcgtct
    12481 gaatatccct atgctttccg cagcaatgga taccgtaacg gaagcgcgcc tggctattgc
    12541 tctggctcag gaaggcggta tcggctttat ccacaaaaac atgtccattg aacgccaggc
    12601 agaagaagtt cgccgtgtga aaaaacacga atctggtgtg gtgactgatc cgcagactgt
    12661 tctgccaacc acgacgctgc gcgaagtgaa agaactgacc gagcgtaacg gttttgcggg
    12721 ctatccggtc gttaccgaag aaaacgaact ggtgggtatt atcaccggtc gtgacgtgcg
    12781 ttttgttacc gacctgaacc agccggttag cgtttacatg acgccgaaag agcgtctggt
    12841 caccgtgcgt gaaggtgaag cccgtgaagt ggtgctggca aaaatgcacg aaaaacgcgt
    12901 tgaaaaagcg ctggtggttg atgacgaatt ccacctgatc ggcatgatca ccgtgaaaga
    12961 cttccagaaa gcggaacgta aaccgaacgc ttgtaaagac gagcaaggcc gtctgcgtgt
    13021 tggtgctgca gttggcgcag gtgcgggtaa cgaagagcgt gttgacgcgc tggttgccgc
    13081 tggcgttgac gttctgctga tcgactcctc ccacggtcac tcagaaggcg ttttgcagcg
    13141 tatccgtgaa acccgtgcta aatatccgga tctgcaaatc atcggcggca acgtggcaac
    13201 agctgcaggt gcacgcgctt tggcagaagc tggttgcagc gcggttaaag tcggtatcgg
    13261 ccctggttct atctgtacga ctcgtatcgt tactggcgta ggtgttccgc agatcactgc
    13321 cgttgctgac gcagttgaag ccctggaagg caccggtatt ccggttatcg cagatggtgg
    13381 tattcgcttc tccggcgaca tcgccaaagc tatcgccgct ggcgcaagcg cggtgatggt
    13441 aggttccatg ctggcgggta ctgaagaatc tccgggtgaa atcgaactct accagggccg
    13501 ttcttataaa tcttaccgtg gtatgggttc cctgggcgcg atgtccaaag gttcctctga
    13561 ccgttatttc cagagcgata acgctgccga caaactggtg ccggaaggta tcgaaggtcg
    13621 cgtagcttat aaaggtcgcc tgaaagagat cattcaccag cagatgggcg gcctgcgctc
    13681 ctgtatgggt ctgaccggct gtggtactat cgacgaactg cgtactaaag cggagtttgt
    13741 acgtatcagc ggtgcgggca ttcaggaaag ccacgttcac gacgtgacca ttactaaaga
    13801 gtccccgaac taccgtctgg gctcctgatt ctcttcgccc gatttcatgt cgggcgattt
    13861 atattatctg tttcacttgc ctcggaataa gcgtcaatga cggaaaacat tcataagcat
    13921 cgcatcctca ttctggactt cggttctcag tacactcaac tggttgcgcg ccgcgtgcgt
    13981 gagctgggtg tttactgcga actgtgggcg tgggatgtga cagaagcaca aattcgtgac
    14041 ttcaatccaa gcggaattat tctttccggc ggcccggaaa gtactactga agaaaacagt
    14101 ccgcgtgcgc cgcagtatgt ctttgaagca ggcgtaccga tattcggcgt ttgctatggc
    14161 atgcagacca tggcaatgca gttgggcggt cacgttgaag cctctaacga acgtgaattt
    14221 ggctacgcgc aggtagaagt cgtaaacgac agcgcactgg ttcgcggtat cgaagatgcg
    14281 ctgaccgcag acggtaaacc gctgctcgat gtctggatga gccacggcga taaagttacc
    14341 gctatcccgt ccgacttcgt caccgtagcc agcaccgaaa gctgcccgtt tgccattatg
    14401 gctaacgaag aaaaacgctt ctatggcgta cagttccacc cggaagtgac tcacacccgc
    14461 cagggtatgc gcatgctgga gcgttttgtg cgtgatatct gccagtgtga agccctgtgg
    14521 acgccagcga aaattatcga cgatgctgta gctcgcatcc gcgagcaggt aggcgacgat
    14581 aaagtcatcc tcggcctctc tggtggtgtg gattcctccg taaccgcaat gctgctgcac
    14641 cgtgctatcg gtaaaaacct gacttgcgta ttcgtcgaca acggcctgct gcgtctcaac
    14701 gaagcagagc aggttctgga tatgtttggc gatcactttg gtcttaacat tgttcacgta
    14761 ccggcagaag atcgcttcct gtcagcgctg gctggcgaaa acgatccgga agcaaaacgt
    14821 aaaatcatcg ggcgcgtttt cgttgaagta ttcgatgaag aagcgctgaa actggaagac
    14881 gtgaagtggc tggcgcaggg caccatctac cctgacgtta tcgaatctgc ggcttctgca
    14941 accggtaaag cacacgtcat caaatctcac cacaacgtgg gcggcctgcc gaaagagatg
    15001 aagatgggcc tggttgaacc gctgaaagag ctgttcaaag acgaagtgcg taagattggt
    15061 ctggagctgg gcctgccgta cgacatgctg taccgtcatc cgttcccggg accaggcctt
    15121 ggcgttcgtg ttctgggtga agtgaagaaa gagtactgtg acctgctgcg ccgtgctgac
    15181 gccatcttca ttgaagaact gcgtaaagcg gacctgtacg acaaagtcag ccaggcgttc
    15241 accgtcttcc tgccagtacg ttccgttggc gtaatgggcg atggtcgtaa gtatgactgg
    15301 gttgtctctc tgcgtgctgt cgaaaccatc gactttatga ccgcacactg ggcgcatctg
    15361 ccgtacgatt tcctcggtcg cgtttccaac cgcattatca atgaagtgaa cggtatttcc
    15421 cgcgtggtgt atgacatcag cggcaagccg ccagctacca ttgagtggga atgattttga
    15481 tccctgcact atgaatgaac aaaaccctcc gttactacag agggtttttt atcttcaaga
    15541 atcataggct tgaagttact aacatcgatt aattaaacca gctgtccgat ttgttctctt
    15601 ctgctttgcc cacgcttttc atcagatcgc gaccgccttc agtcatattt ctgtttgcgt
    15661 cagcttcaga ttgcaccaca tcggtttgcg cagctttgtg cttcagttcc tgatcgataa
    15721 attcgttttc gcgcttaacg cgggcttctt ctttcgccag cgccagtttt tgtttctgaa
    15781 tctctaagct gcgtagctca tcttcataac tttgatcgcg ttttttgtcc gcagtggctt
    15841 cggcgtccag tttatcctga cgagctttct tatttgccgc tgccgttgcc gctcttttgt
    15901 tcgcggcggc ctgggcattt gcgcgacgtt gcttctcttg ctggatttcc ctgttgcgct
    15961 ccgcgaccca ttcgtcatgc tgcctttgct cttcattttt accttgctgt tccgcttctg
    16021 cgacagcaga gagttgatcc tgcaatgatg aggctatagc cggatagctt aaggaggcta
    16081 agatggcgca aagaaaaact ttcttcatga ctcctcctga ttattagctc ttttcaggac
    16141 atttggtatt tggctgaata cgcgtttcgt tatacgttgt ggtaataaca acggctaaac
    16201 ctgtcgtaaa ctggcactct ttaccctcct gggtggaggt atacactttg gtgccttcct
    16261 tatatgttaa agaaacacct tccactaagg ttttatcatt caccatagaa cccgctgccg
    16321 cgcctacagc tccgccgcca actgcccctg ccgtcgttcc ggaattgctg ccagaaccga
    16381 cgttgtggcc gataacaccg ccagcgactg cgccaataag cgcgccgaag gcttgtgcgt
    16441 tccgtttatt ttgggagttg tctacggcaa cttttgcggg aagaatggaa ataatattaa
    16501 cggttttagt ttcttgtttg gtattcagtt gatcggtttg ataaacatcg gcggcatgat
    16561 cgtcagcatt tgactggcat cctgccagag tgaatgacgc taacattgcc acaggcagaa
    16621 gacatttttt aaatttcatc actattcctt gttatacaca ttacgaaaaa taagacattt
    16681 aagatcatca aaagaggaaa gtaaatgtat tgcgtataaa gggatagatt aaatttatta
    16741 aaattcacgc agggaataat ttataaaaat atatcattac aacatatata ttaattttat
    16801 atttatttcg cagccaaaca ggataaacat cagtcaggac aaagacgtaa aatttaagat
    16861 gaaagaatat ctgccgcttt gataatccca aagagcaaaa gcatccgaca gcgaagtgcc
    16921 gttatgttct gtgaaagtgg agaaggtaac cgctaacgtt gtaccggtga ttcgacggag
    16981 agtgtggatt gctgagcttc ttttttgctc tgatggtgct gccaggcacc gacggacgag
    17041 tagataaaac ggccaaagaa gaagataaag ctgatgagca gtacgatacg ggtcatgcga
    17101 ctgttaaatc ggtgtcgttt tcgcatacta gttgcctgac tcacaaaagg ttccttgaag
    17161 tatgtcccac gctgtggacg gtacttacat taaggcacaa caggacaaaa tggtcaattc
    17221 ttcgttatgt aaaaaagcgt cagtggatac atattttaat gttatggaag ttaatttaat
    17281 tatttacaat gatgatgtaa taatgatgaa ttaacatgat aaacgtaata attcattaat
    17341 cagggttatt ttgtttgata tatatcaatt ggaactttca tatgacactt agaatcaatg
    17401 ctctctatag tgatgaataa tcatcattcg aagtcaggtg ggatgcctgt ctgaatacac
    17461 ctccttcagg atgtggggga ttctgctgag catctatgaa actgaatgca acttatataa
    17521 aaatacgtga taaatggtgg gggcttccgc tgttcctgcc ttctttaatc ttgcccattt
    17581 tcgcccacat taatactttc gcgcatattt cttccggtga ggtttttctc ttttatctgc
    17641 ctttggcact gatgatcagc atgatgatgt ttttcagctg ggcggcattg ccagggatca
    17701 ccttagggat ttttgtccgc aaatatgcag agctgggttt ttacgaaacg ctctcattaa
    17761 cggctaattt tattatcatt atcattctct gttggggcgg ttacagggtc tttactcccc
    17821 ggcgtaacaa cgtttcacat ggtgataccc gtttaatttc ccagcgtata ttctggcaga
    17881 tagtgtttcc tgcaacgctg tttctgatac ttttccagtt tgctgcattt gtaggattac
    17941 tggcgagcag agaaaatctg gtcggtgtca tgccttttaa cctcgggact ttaatcaatt
    18001 atcaggcctt actggtgggt aatctgatcg gtgtcccgct gtgctacttc atcattcggg
    18061 tagtgcgaaa tccattttat ttacgtagct attattcgca attaaaacag caggttgatg
    18121 ccaaagtcac caaaaaagag ttcgcgctct ggctactggc attaggtgct ttattgttgc
    18181 tgttatgcat gccgttaaat gaaaaaagca caatttttag caccaattat accttgtcat
    18241 tattgctgcc cctgatgatg tggggagcaa tgcgctatgg ttataagctg atttcgctgc
    18301 tctgggcggt cgtgttgatg atcagcatcc acagctatca aaattacatt cccatttatc
    18361 ctggctatac cacgcagctg accataacct cctccagtta tctggtattc tcttttattg
    18421 tcaattatat ggctgtactg gcaactcgtc agcgagcggt agtcagacgc attcagcggc
    18481 ttgcgtatgt ggacc
//
LOCUS       DAJVPT010000082        18465 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVPT010000082 DAJVPT010000000
VERSION     DAJVPT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1354
                     /gene="ravA"
                     /locus_tag="OQM47_002774"
     CDS             <1..1354
                     /gene="ravA"
                     /locus_tag="OQM47_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1480865.1"
                     /translation="GIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALK
                     DEGRYERLTSGYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLL
                     VAASNELPEADSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVT
                     DEEYERWQKEIGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFF
                     SGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHL
                     QLQQQQSDKTALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISF
                     ERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAE
                     GLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC
                     "
     gene            1348..2799
                     /gene="viaA"
                     /locus_tag="OQM47_002775"
     CDS             1348..2799
                     /gene="viaA"
                     /locus_tag="OQM47_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1480866.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2804..3796)
                     /gene="asnA"
                     /locus_tag="OQM47_002776"
     CDS             complement(2804..3796)
                     /gene="asnA"
                     /locus_tag="OQM47_002776"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1480867.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            3948..4406
                     /gene="asnC"
                     /locus_tag="OQM47_002777"
     CDS             3948..4406
                     /gene="asnC"
                     /locus_tag="OQM47_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1480868.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4496..4939
                     /gene="mioC"
                     /locus_tag="OQM47_002778"
     CDS             4496..4939
                     /gene="mioC"
                     /locus_tag="OQM47_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1480869.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5318..7207
                     /gene="mnmG"
                     /locus_tag="OQM47_002779"
     CDS             5318..7207
                     /gene="mnmG"
                     /locus_tag="OQM47_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1480870.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7271..7894
                     /gene="rsmG"
                     /locus_tag="OQM47_002780"
     CDS             7271..7894
                     /gene="rsmG"
                     /locus_tag="OQM47_002780"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1480871.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8511..8891
                     /gene="atpI"
                     /locus_tag="OQM47_002781"
     CDS             8511..8891
                     /gene="atpI"
                     /locus_tag="OQM47_002781"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1480872.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            8900..9715
                     /gene="atpB"
                     /locus_tag="OQM47_002782"
     CDS             8900..9715
                     /gene="atpB"
                     /locus_tag="OQM47_002782"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1480873.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9762..10001
                     /gene="atpE"
                     /locus_tag="OQM47_002783"
     CDS             9762..10001
                     /gene="atpE"
                     /locus_tag="OQM47_002783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1480874.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10063..10533
                     /gene="atpF"
                     /locus_tag="OQM47_002784"
     CDS             10063..10533
                     /gene="atpF"
                     /locus_tag="OQM47_002784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1480875.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10548..11081
                     /gene="atpH"
                     /locus_tag="OQM47_002785"
     CDS             10548..11081
                     /gene="atpH"
                     /locus_tag="OQM47_002785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1480876.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11094..12635
                     /gene="atpA"
                     /locus_tag="OQM47_002786"
     CDS             11094..12635
                     /gene="atpA"
                     /locus_tag="OQM47_002786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1480877.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12686..13549
                     /gene="atpG"
                     /locus_tag="OQM47_002787"
     CDS             12686..13549
                     /gene="atpG"
                     /locus_tag="OQM47_002787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1480878.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13576..14958
                     /gene="atpD"
                     /locus_tag="OQM47_002788"
     CDS             13576..14958
                     /gene="atpD"
                     /locus_tag="OQM47_002788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1480879.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            14979..15398
                     /gene="atpC"
                     /locus_tag="OQM47_002789"
     CDS             14979..15398
                     /gene="atpC"
                     /locus_tag="OQM47_002789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1480880.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15749..17119
                     /gene="glmU"
                     /locus_tag="OQM47_002790"
     CDS             15749..17119
                     /gene="glmU"
                     /locus_tag="OQM47_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1480881.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17281..>18465
                     /gene="glmS"
                     /locus_tag="OQM47_002791"
     CDS             17281..>18465
                     /gene="glmS"
                     /locus_tag="OQM47_002791"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1480882.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAG"
BASE COUNT         4547 a         4625 c         4881 g         4412 t
ORIGIN      
        1 aggtattgcc aaaagtttga tcgcccggcg cttaaaattc gcctttcaga atgcccgcgc
       61 gtttgaatat ctgatgaccc gcttctccac gccggaagaa gtttttggtc ccctttctat
      121 tcaggcgcta aaagatgaag ggcgctatga acgtttaacc agcggttacc tgccggaagc
      181 agaaatcgtc tttctggatg agatctggaa agcgggcccg gcaattctta ataccttgct
      241 caccgccatt aacgagcgcc agttccgcaa cggtgcacac gtagaaaaaa tcccgatgcg
      301 tctactggtg gcggcctcca acgagctgcc ggaagcagac agcagtctgg aagcgttata
      361 tgaccgcatg ctgattcgtc tatggttaga taaagtgcaa gataaagcga atttccgctc
      421 catgctgacc agtcaacagg atgaaaacga caatccggtt cctgatgcct tgcaggtcac
      481 agatgaagaa tatgaacgct ggcagaaaga gattggtgaa attacgctgc ccgatcatgt
      541 atttgagctg atttttattc tgcgccagca actggataaa ttaccggatg cgccttatgt
      601 ctcggatcgt cgctggaaaa aagcgatccg cttattgcag gccagcgcct tttttagcgg
      661 tcgcagtgct gttgccccgg ttgatctcat tttgctgaaa gattgcctgt ggtatgacgc
      721 gcaaagcctg aatttgatac aacaacaaat tgatgtattg atgaccggtc atgcctggca
      781 acagcaaggg atgttgaccc gcctgggcgc gattgtgcaa cgtcacctgc aactccagca
      841 gcaacaaagc gataaaacag ccttaacgtt aattcgtctg ggcggcattt tcagccgtcg
      901 tcagcagtat caacttcctg ttaacgttac tgcttccact ctgactctgc tgctgcaaaa
      961 accgttaaaa ctgcatgata tggaagtggt tcatatctcc tttgagcgta gcgcgctgga
     1021 acagtggctg agtaaaggtg gtgaaattcg cggcaaactg aacggtatcg gctttgccca
     1081 gaaactgaat ctggaagttg atagcaccca acatcttgtt gtacgcgatg taagtttaca
     1141 aggcagtacg ctggcacttc ccggttcatc ggctgaaggt ctgccaggtg aaataaaaca
     1201 acaactggaa gagcttgaaa gcgactggcg taagcaacac gctttattca gcgaacagca
     1261 aaaatgtctg tttatccctg gcgactggtt aggtcgcatt gaagacagcc tacaggatgt
     1321 cggtgcacag attcgtcagg cacaacaatg ctaacgctgg atacgcttaa tgtgatgctg
     1381 gccgtcagcg aagagggatt gatcgaagag atgatcatcg cactgctggc ctcaccgcag
     1441 ctggcggtct tctttgaaaa attcccacga ctgaaggcgg caatcactga tgatgttccc
     1501 cgctggcgtg aggcgctgcg cagtcggctg aaagatgccc gagtcccgcc ggaactcacc
     1561 gaagaggtga tgtgctatca gcaaagccag ctcctctcca cgccgcagtt tattgtgcag
     1621 ctaccacaga tcctggactt actgcatcgt ctgaattccc catgggcaga acaagcccgg
     1681 cagttggttg atgctaacag cacaataact tcagcgttac acacgctttt tctccagcgt
     1741 tggcgtttaa gtctgatcgt gcaagcaacg acgttaaatc aacagctatt agaagaagaa
     1801 cgcgaacaac tgttaagtga agttcaggaa cgcatgacgc tgagcggaca acttgaaccg
     1861 attctcgcag ataacaatac cgcagctggt cgtctgtggg atatgagcgc cggtcaactt
     1921 aaacgtggcg actatcagtt tattgtgaaa tacggtgaat ttcttaacga acagccggaa
     1981 ctgaaacgcc tggcagagca gctggggcgt tctcgggaag ccaaatcaat accgcgcaac
     2041 gatgcgcaga tggaaacctt ccgcaccatg gtgcgcgaac cggcgacggt tcctgagcag
     2101 gttgatggtc tgcaacaaag cgatgatatt ttacgtctcc tgccgccaga actggcgaca
     2161 ctagggataa cggaactgga gtatgagttt taccgtcggc tggtggaaaa acagttgctc
     2221 acctatcgcc tgcacggtga gtcgtggcgt gaaaaagtgt tcgaacgtcc ggtggtacat
     2281 aaagattacg atgaacagcc gcgcgggccg tttattgtct gtgtggatac ttccggctca
     2341 atgggcggct ttaatgaaca gtgtgcgaaa gcgttctgcc tggctttgat gcgcattgct
     2401 ctcgctgaaa accggcgctg ttatattatg ctattttcca ccgagatcgt ccgttatgag
     2461 ctttcaggcc cacaaggcat cgaacaagca atccgttttt taagccagca gtttcgtggc
     2521 ggcaccgatc ttgccagttg ttttcgcgcc attatggaac gcttgcaaag cagggaatgg
     2581 tttgatgccg atgcggtggt gatttctgat tttatcgctc agcggttgcc tgacgacgtg
     2641 acgagtaaag tgaaagagct gcagcgggta catcagcatc gctttcatgc cgtggcgatg
     2701 tcggcacacg gcaaacccgg catcatgcgc attttcgatc atatctggcg ctttgatacc
     2761 gggatgcgaa gccgcctgct cagacgctgg cggcgataaa ttattacagc agagaaggga
     2821 cgctctcgcg aacagcagct ggccatactc cacactgaac ctggccgata tgcggcagtt
     2881 gcagcagcag catagtcaaa cgagactggc cgataccgcc gccgatggtc tgcggcattt
     2941 caccgcgcag cagcgcctga tgccattcca gctgcagacg atcttcatca ccggtcagcg
     3001 ccagctgatg cttcagcgtg tcggcatcca cgcggatccc catggaagaa agctcaaacg
     3061 catcttccag taccgggttc cacaccagaa tatcgccgtt caggcccgca tggcccagct
     3121 ctgacagggt gctccagtca tcataatccg gtgcgcgcac gtcgtgacga tgaccatcac
     3181 tcaacttgcc gccaatcccg acgaggaata ccgcgccaag atctttcgca atcgcccgct
     3241 cacgcccttt ggcatcgaga tccggataac gagacagtaa ctcctggctg tgtacgaagt
     3301 gaatctgatc cggcaggaac ggtgccaggc caaactcttc gttaaccgca gcttcggttg
     3361 ctttaattcc cgcccagatc gcctctaccg tgcttttcag agtcgagagt tgacgctcac
     3421 cgtcgcccat tacgcgttcc cagtcccact ggtcaacata gaccgagtgc aacggagaaa
     3481 gacggtcttc atcggggcga agggctttca tgtgcgtgta cagcccttcg cccgcgctga
     3541 agtcgtgttg ccctaaggtc tgacgtttcc acttcgccag tgaatgaacc acttcgaact
     3601 gggcatcagg cagagctttc acttttacct gcaccgcttt ttcacagccc gacaagttat
     3661 cctgcgtgcc atcccccaca cggctaagaa tcggcgcctg gacttcgatc agccccagac
     3721 gttcttccag ttggcgagaa aagtgagatt tcacgaagct aatttggcgt tgtttggcaa
     3781 tgtaagcggt tttcattttt tatactcctg cgtcctgttg cttatgatta agcaacaaaa
     3841 accgacacac atgcaataat cattcaataa aaagcctaac acacttttga ttcattaaaa
     3901 aagacggcta aaatagaatg aatcatcaat ctgcataaga aaatcctatg gaaaattatc
     3961 tgatcgacaa tctggaccgt ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt
     4021 acgccgaact ggcgaaacaa tttggcgtca gtccggggac gattcacgtt cgagtagaga
     4081 aaatgaagca ggcggggatc attaccgggg cgcgtattga tgtcagcccg aagcagctcg
     4141 gttatgacgt aggctgcttt atcggcatta tattaaagag cgccaaagac tacccttccg
     4201 cgctggcaaa gctggaaagc cttgatgaag tcactgaagc ctattacaca accggccact
     4261 acagcatctt tataaaagtt atgtgccgtt cgatcgacgc tctccagcat gtacttatca
     4321 acaagatcca aacaattgat gaaattcagt ccaccgagac attgatcgtc ctgcaaaacc
     4381 cgatcatgcg taccatcaag ccctgatcgg cttttttaat cccatacttt tccacaggta
     4441 gatcccaacg cgttcacagc gtacaatacg ccactcttaa taaaggtggc ggtttatggc
     4501 agatatcact cttatcagcg gcagcaccct cggcggtgcc gaatatgtag cagaacacct
     4561 ggctgaaaag ctggaagagg tgggttttac caccgaaacg ctgcacggtc cgctgttgga
     4621 agatttatct gcctcgggga tctggctggt tatcagctcc acccacggtg ccggagatat
     4681 tccggacaac ctttctcctt tctatgaagc attgcaggaa cagaagcccg atctttctgc
     4741 ggtccgcttt ggcacaatcg gtattggcag tcgtgaatat gacacctttt gtggggctat
     4801 cgataaactc gaggcagaac tcaaaaattc cggtgcaaaa cagacaggcg aaacactgaa
     4861 gatcaacatt cttgatcacg acattccgga agatccggca gaagaatggc tgggatcgtg
     4921 gattaattta ctcaaataag tatacagatc gtgcgatcta ctgtggataa ctctgtcagg
     4981 aagcttggat caaccggtag ctatccaaag aacaaccgtt gttcagtttt tgagttgtgt
     5041 ataacccctc attctgatcc cagcttatac ggtccaggat caccgatcat tcacagttaa
     5101 tgatcctttc caggttgttg atcttaaaaa tcggatcctt gttatccaca gggcagtgcg
     5161 atcctaataa gagatcacaa tagaacagat ctctaaataa atagatcttc tttttaatac
     5221 ccaggatccc aggtctttct caagccgaca aagttgagta gaatccacgg cccgggcttc
     5281 aatccatttt cataccgcgt tatgcgaggc aatcaccatg ttttatccgg atccttttga
     5341 cgtcatcatc attggcgggg gtcatgcagg caccgaggcc gcgatggctg cggcgcgtat
     5401 gggtcaacag actctgcttt tgacacacaa tatcgacact ctggggcaga tgagctgcaa
     5461 cccggcgatc ggcggtattg ggaagggaca tctggtaaaa gaagtggatg cactcggcgg
     5521 tctgatggcg aaagcgatcg atcaagcggg tatccagttt aggatactaa acgcaagcaa
     5581 aggaccggcg gttcgcgcta cccgagctca ggcggatcgt gtgcgctacc gtcaggcggt
     5641 acgtacggcg ctggagaacc aaccgaacct gatgatcttc cagcaggcgg ttgaagatct
     5701 tattgtcgaa aacgatcgcg tggtcggtgc tgttacccaa atgggactga agttccgtgc
     5761 caaagctgtg gtgctcaccg ttgggacgtt cctcgacggc aaaattcata tcggtctgga
     5821 taattacagc ggtggccgtg ctggcgatcc gccatccatt ccgctctctc gccgtttgcg
     5881 tgaactgccg ctgcgcgttg gtcgtctgaa aaccggtact ccgccgcgta ttgatgctcg
     5941 aaccatcgac tttagcgtgc tggcgcaaca gcatggcgat aacccaatgc cggtattctc
     6001 gtttatgggc aatgcgtccc tgcatcccca gcaggtgccg tgttatatca ctcataccaa
     6061 cgagaaaacc catgatgtga tccgcagtaa cctcgatcgt agcccaatgt atgcaggggt
     6121 gatcgaaggt gtcggcccac gctactgccc gtcgatcgaa gacaaagtca tgcgcttcgc
     6181 agacagaaat cagcatcaga tcttccttga accggaagga ctgacctcta atgaaattta
     6241 tccgaacggt atctccacca gcctgccgtt cgatgtgcag atgcaaatcg tccgctctat
     6301 gcaggggatg gaaaacgcga agatcgtgcg tccgggttat gccattgagt atgacttctt
     6361 cgatcctcgc gacctgaaac cgacgctgga gagcaagttt atccaggggc tgttctttgc
     6421 tggtcagatt aacggcacta ccggttacga agaagccgct gcgcaaggtt tgctggccgg
     6481 tcttaacgct gcccgtctgt ctgctgacaa agaaggttgg gctccggcac gttctcaggc
     6541 gtatctcggc gtactggttg atgacctgtg cactttagga accaaagaac cgtatcgtat
     6601 gtttacttcg cgcgcagaat atcgtctgat gctgcgcgaa gataatgcgg atctgcgttt
     6661 gactgaaatc ggtcgtgaac tgggcctggt ggatgacgaa cgttgggcgc gctttaacga
     6721 gaaacttgag aatatcgagc gtgagcgtca gcgtctgaaa tcgacctggg taaccccgtc
     6781 ggcggaagct gcagccgaag tgaatgctca cctgactgcg ccgctttccc gtgaagccag
     6841 tggtgaagat ctgctgcgtc gtccggaaat gacttatgaa aaattaacca cgctgacgcc
     6901 gtttgcccct gcgttgacag acgaacaggc ggcggaacag gttgagattc aggttaaata
     6961 cgaaggttat atcgcgcgcc agcaagatga gatcgaaaag cagctgcgta acgagaacac
     7021 cctgctaccc gcgacactgg attaccgcca gatatccggt ctttctaacg aagtgatcgc
     7081 caaacttaac gatcacaaac cggcctctat cggccaggct tcgcgtattt ctggcgtcac
     7141 gcctgcggcc atctccattc tgctggtgtg gctgaaaaaa cagggtatgc tgcgtcgtag
     7201 cgcataacgc attaaaaatg cctggtaagc acccgcttac caggcaacgc atcaagaaca
     7261 ggtaatcacc gtgctcaaca aactctcctt actgctgaaa gacgcaggta tttcgcttac
     7321 tgatcaccag aaaaaccagc ttattgccta cgtgaatatg ctgcataaat ggaacaaagc
     7381 gtacaacctg acttcggtcc gcgatcctaa tgagatgctg gtacgccata ttctcgatag
     7441 cattgtggtg gcaccgtatc tgcaaggtga acggtttatc gatgtcggca ccggaccagg
     7501 actgccaggc attccactct ctatcgtgcg tcctgaagcc catttcactc tgttggatag
     7561 ccttggtaaa cgcgtgcgtt tccttcgtca ggtgcaacat gagcttaaac tggagaatat
     7621 tgaaccagta cagagcaggg tagaagagtt tccttcagag ccgccatttg atggcgtaat
     7681 tagccgcgct tttgcctctc tgaacgatat ggtgagctgg tgccaccatc ttcctggtga
     7741 gcaaggccgt ttctacgcgc tgaaagggca aatgccggaa gatgaaatcg ctttgttgcc
     7801 cgaagaatat caggtcgaat cagtggttaa acttcaggtt ccagccctgg atggcgaacg
     7861 tcatctggtg gtgattaaag caaataaaat ttaattttta tcaaaaaaat cataaaaaat
     7921 tgaccggtta gactgttaac aacaaccagg ttttctactg atataactgg ttacatttaa
     7981 cgccacgttc actcttttgc atcaacaaga taacgtggct ttttttggta agcagaaaat
     8041 aagtcattag tgaaaatatc agtctgctaa aaatcggcgc taagaaccat cattggctgt
     8101 taaaacatta ttaaaaatgt caatgagtgg tttttgttgt gtaaatgtta tttattaaaa
     8161 cagtatctgt ttttagactg aaatatcata aacttgtaaa ggcatcattt gccaagtaaa
     8221 taaatatgct gtgcgcgaac atgcgcaata tgtgatctga agcacgcttt atcaccagtg
     8281 tttacgcgtt atttacagtt tttcatgatc gaacaggggt agcggaaaag tcgcaattgt
     8341 atgcactgga aaaatattta aacatttatt caccttttgg ctacttattg tttgaaatca
     8401 cgggggcgca ccgtataatt tgaccgcttt ttgatgcttg actctaagcc ttaaagaaag
     8461 ttttatacga cacgcggcat acctcgaagg gagcaggagt gaaaaacgtg atgtctgtgt
     8521 cgctcgtgag tcgaaacgtt gctcggaagc ttctgctcgt tcagttactg gtggtgatag
     8581 caagtggatt gctgttcagc ctcaaagacc ccttctgggg cgtctctgca ataagcgggg
     8641 gcctggcagt ctttctgcct aacgttttgt ttatgatatt tgcctggcgt caccaggcgc
     8701 atacaccagc gaaaggccgg gtggcttgga cattcgcatt tggcgaagct ttcaaagttc
     8761 tggcgatgtt ggtgttactg gtggtggcgt tggcggtttt aaaggcggta ttcttgccgc
     8821 tgatcgttac gtgggttttg gtgctggtgg ttcagatact ggcaccggct gtaattaaca
     8881 acaaagggta aaaggcatca tggcttcaga aaatatgacg ccgcaggatt acataggaca
     8941 ccacctgaat aaccttcagc tggacctgcg tacattctcg ctggtggatc cacaaaaccc
     9001 cccagccacc ttctggacaa tcaatattga ctccatgttc ttctcggtgg tgctgggtct
     9061 gttgttcctg gttttattcc gtagcgtagc caaaaaggcg accagcggtg tgccaggtaa
     9121 gtttcagacc gcgattgagc tggtgatcgg ctttgttaat ggtagcgtga aagacatgta
     9181 ccatggcaaa agcaagctga ttgctccgct ggccctgacg atcttcgtct gggtattcct
     9241 gatgaatctg atggatttac tgcctatcga cttgctgccg tacattgctg aacatgtact
     9301 gggtctgcct gcactgcgtg tggttccgtc tgcggacgtg aacgtaacgc tgtctatggc
     9361 actgggcgta tttatcctga ttctgttcta caacatcaaa atgaaaggca tcggcggctt
     9421 cacgaaagag ttgacgctgc agccgttcaa tcactgggcg ttcattcctg tcaacttaat
     9481 ccttgaaggg gtaagcctgc tgtccaaacc agtttcactc ggtttgcgac tgttcggtaa
     9541 catgtatgcc ggtgagctga ttttcattct gattgctggt ctgttgccgt ggtggtcaca
     9601 gtggatcctg aatgtgccgt gggccatttt ccacatcctg atcattacgc tgcaagcctt
     9661 catcttcatg gttctgacga tcgtctatct gtcgatggcg tctgaagaac attaatttac
     9721 caacattact acgttttaac tgaaacaaac tggagactgt catggaaaac ctgaatatgg
     9781 atctgctgta catggctgcc gctgtgatga tgggtctggc ggcaatcggt gctgcgatcg
     9841 gtatcggcat cctcgggggt aaattcctgg aaggcgcagc gcgtcaacct gatctgattc
     9901 ctctgctgcg tactcagttc tttatcgtta tgggtctggt ggatgctatc ccgatgatcg
     9961 ctgtaggtct gggtctgtac gtgatgttcg ctgtcgcgta gtaagcgttg cttttattta
    10021 aagagcaata tcagaacgtt aactaaatag aggcattgtg ctgtgaatct taacgcaaca
    10081 atcctcggcc aggccatcgc gtttgtcctg ttcgttctgt tctgcatgaa gtacgtatgg
    10141 ccgccattaa tggcagccat cgaaaaacgt caaaaagaaa ttgctgacgg ccttgcttcc
    10201 gcagaacgag cacataagga ccttgacctt gcaaaggcca gcgcgaccga ccagctgaaa
    10261 aaagcgaaag cggaagccca ggtaatcatc gagcaggcga acaaacgccg ctcgcagatt
    10321 ctggacgaag cgaaagctga ggcagaacag gaacgtacta aaatcgtggc ccaggcgcag
    10381 gcagaaattg aagccgagcg taaacgtgcc cgtgaagagc tgcgtaagca agttgctatc
    10441 ctggctgttg ctggcgccga gaagatcatc gaacgttccg tggatgaagc tgctaacagc
    10501 gacatcgtgg ataaacttgt cgctgaactg taaggaggga ggggctgatg tctgaattta
    10561 ttacggtagc tcgcccctac gccaaagcag cttttgactt tgccgtcgaa caccaaagtg
    10621 tagaacgctg gcaggacatg ctgacgtttg ccgccgaggt aaccaaaaac gaacaaatgg
    10681 cagagcttct ctctggcgcg cttgcgccag aaacgctcgc cgagtcgttt atcgcagttt
    10741 gtggtgagca actggacgaa aacggtcaga acctgattcg ggtaatggct gaaaatggtc
    10801 gtcttaacgc gctcccggat gttctggagc agtttattca ccttcgtgcc gtgagtgagg
    10861 ctaccgctga ggtagacgtc atttccgctg ccgcactgag tgaacaacag ctcgcgaaaa
    10921 tttctgctgc gatggaaaaa cgtctgtcac gcaaagttaa gctgaattgc aaaatcgata
    10981 agtctgtaat ggctggcgtt atcatccgat cgggtgatat ggtcattgat ggcagcgtac
    11041 gcggtcgtct tgagcgcctt gcagacgtct tgcagtctta aggggactgg agcatgcaac
    11101 tgaattccac cgaaatcagc gaactgatca agcagcgcat tgctcagttc aatgttgtga
    11161 gtgaagctca caacgaaggt actattgttt ctgtaagtga cggtgttatc cgcattcacg
    11221 gcctggccga ttgtatgcag ggtgaaatga tctccctgcc gggtaaccgt tacgctatcg
    11281 cactgaacct cgagcgcgac tctgtaggtg cggttgttat gggtccgtac gctgaccttg
    11341 ccgaaggcat gaaagttaag tgtactggcc gtatcctgga agttccggtt ggccgtggcc
    11401 tgctgggccg tgtggttaac actctgggtg caccaatcga cggtaaaggt ccgctggatc
    11461 acgacggctt ctctgctgta gaagcaatcg ctccgggcgt tatcgaacgt cagtccgtag
    11521 atcagccggt acagaccggt tataaagccg ttgactccat gatcccaatc ggtcgtggtc
    11581 agcgtgaatt gatcatcggt gaccgtcaga caggtaaaac cgcactggct atcgatgcca
    11641 tcatcaacca gcgcgattcc ggtatcaaat gtatctatgt cgctatcggc cagaaagcgt
    11701 ccaccatttc taacgtggta cgtaaactgg aagagcacgg cgcactggct aacaccatcg
    11761 ttgtggtagc aaccgcgtct gaatccgctg cactgcaata cctggcaccg tatgccggtt
    11821 gcgcaatggg cgaatacttc cgtgaccgcg gtgaagatgc gctgatcatt tacgatgacc
    11881 tgtctaaaca ggctgttgct taccgtcaga tctccctgct gctccgtcgt ccgccaggac
    11941 gtgaagcatt cccgggcgac gttttctacc tccactctcg tctgctggag cgtgctgcac
    12001 gtgttaacgc cgaatacgtt gaagccttca ccaaaggtga agtgaaaggg aaaaccggtt
    12061 ctctgaccgc actgccgatt atcgaaactc aggcgggtga cgtttctgcg ttcgttccga
    12121 ccaacgtaat ctccattacc gatggtcaga tcttcctgga aaccaacctg ttcaacgccg
    12181 gtattcgtcc tgcggttaac ccgggtattt ccgtatcccg tgttggtggt gcagcacaga
    12241 ccaagatcat gaaaaaactg tccggtggta tccgtaccgc tctggcacag tatcgtgaac
    12301 tggcagcgtt ctctcagttt gcatccgacc ttgacgatgc aacacgtaag cagcttgacc
    12361 acggtcagaa agtgaccgaa ctgctgaaac agaaacagta tgcgccgatg tccgttgcgc
    12421 agcagtctct ggttctgttc gcagcagaac gtggttacct ggcggatgtt gaactgtcga
    12481 aaattggcag cttcgaagcc gctctgctgg cttacgtcga ccgtgatcac gctccgttga
    12541 tgcaagagat caaccagacc ggtggctaca acgacgaaat cgaaggcaag ctgaaaggca
    12601 tcctcgattc cttcaaagca acccaatcct ggtaacgtct ggaggcttgc cttagggcag
    12661 gccgcaaggc attgaggaga agctcatggc cggcgcaaaa gagatacgta gtaagatcgc
    12721 aagcgtccag aacacgcaaa agatcactaa agcgatggag atggtcgccg cttccaaaat
    12781 gcgtaaatcg caggatcgca tggcggccag ccgtccttat gcagaaacca tgcgcaaagt
    12841 gattggtcac cttgcacacg gtaatctgga atataagcac ccttacctgg aagaccgcga
    12901 cgttaaacgc gtgggctacc tggtggtgtc gaccgaccgt ggtttgtgcg gtggtttgaa
    12961 cattaacctg ttcaaaaaac tgctggcgga aatgaagacc tggaccgaca aaggcgttca
    13021 atgcgacctc gcaatgatcg gctcgaaagg cgtgtcgttc ttcaactccg tgggcggcaa
    13081 tgttgttgcc caggtcaccg gcatggggga taacccttcc ctgtccgaac tgatcggtcc
    13141 ggtaaaagtg atgttgcagg cctacgacga aggccgtctg gacaagcttt acattgtcag
    13201 caacaaattt attaacacca tgtctcaggt tccgaccatc agccagctgc tgccgttacc
    13261 ggcatcagat gatgatgatc tgaaacataa atcctgggat tacctgtacg aacccgatcc
    13321 gaaggcgttg ctggataccc tgctgcgtcg ttatgtcgaa tctcaggttt atcagggcgt
    13381 ggttgaaaac ctggccagcg agcaggccgc ccgtatggtg gcgatgaaag ccgcgaccga
    13441 caatggcggc agcctgatta aagagctgca gttggtatac aacaaagctc gtcaggccag
    13501 cattactcag gaactcaccg agatcgtctc gggggccgcc gcggtttaaa caggttattt
    13561 cgtagaggat ttaagatggc tactggaaag attgtccagg taatcggcgc cgtagttgac
    13621 gtcgaattcc ctcaggatgc cgtaccgcgc gtgtacgatg ctcttgaggt gcaaaatggt
    13681 aatgagcgtc tggtgctgga agttcagcag cagctcggcg gcggtatcgt gcgtaccatc
    13741 gcaatgggtt cctccgacgg tctgcgtcgc ggtctggatg taaaagacct cgaacacccg
    13801 atcgaagtcc cggtaggtaa agcgactctg ggccgtatca tgaacgtact gggtgaaccg
    13861 gtcgacatga aaggcgagat cggtgaagaa gagcgttggg cgattcaccg cgcagcacct
    13921 tcctacgaag agctgtcaaa ctctcaggaa ctgctggaaa ccggtatcaa agttatcgac
    13981 ctgatgtgtc cgttcgctaa gggcggtaaa gttggtctgt tcggtggtgc gggtgtaggt
    14041 aaaaccgtaa acatgatgga gctcattcgt aacatcgcga tcgagcactc cggttactct
    14101 gtgtttgcgg gcgtaggtga acgtactcgt gagggtaacg acttctacca cgaaatgacc
    14161 gactccaacg ttatcgacaa agtatccctg gtgtatggcc agatgaacga gccgccggga
    14221 aaccgtctgc gcgttgctct gaccggtctg accatggctg agaaattccg tgacgaaggt
    14281 cgtgacgttc tgctgttcgt tgacaacatc tatcgttaca ccctggccgg tacggaagta
    14341 tccgcactgc tgggccgtat gccttcagcg gtaggttatc agccgaccct ggcggaagag
    14401 atgggcgttc tgcaggaacg tatcacctcc accaaaactg gttctatcac ctccgtacag
    14461 gcggtatatg tacctgcgga tgacttgact gacccgtctc cggcaaccac ctttgcgcac
    14521 cttgacgcaa ccgtggtact gagccgtcag atcgcgtctc tgggtatcta cccggccgtt
    14581 gacccgctgg actccaccag ccgtcagctg gacccgctgg tggttggtca ggaacactac
    14641 gacaccgcgc gtggcgttca gtccatcctg caacgttatc aggaactgaa agacatcatc
    14701 gccatcctgg gtatggatga actgtctgaa gaagacaaac tggtggtagc gcgtgctcgt
    14761 aagatccagc gcttcctgtc ccagccgttc ttcgtggcag aagtattcac cggttctccg
    14821 ggtaaatacg tctccctgaa agacaccatc cgtggcttta aaggcatcat ggaaggcgaa
    14881 tacgatcacc tgccggagca ggcgttctac atggtcggtt ccatcgaaga agctgtggaa
    14941 aaagccaaaa aactttaacg ccttaatcgg agggtgatat ggcaatgact taccacctgg
    15001 acgtcgtcag cgcagagcaa caaatgttct ctggtctggt cgagaaaatc caggtaacgg
    15061 gtagcgaagg tgaactgggg atctaccctg gccacgcacc gctgctcacc gccattaagc
    15121 ctggtatgat tcgcatcgtg aaacagcacg gtcacgaaga gtttatctat ctgtctggcg
    15181 gcattcttga agtgcagcct ggcaacgtga ccgttctggc cgacaccgca attcgcggcc
    15241 aggatctcga cgaagcgcga gccatggaag cgaaacgtaa ggctgaagag cacattagca
    15301 gctctcacgg cgacgtagat tacgctcagg cgtctgcgga actggccaaa gcgatcgcgc
    15361 agctgcgcgt tatcgagttg accaaaaaag cgatgtaaca ccggcttgaa aagcacaaaa
    15421 gccagtctgg aaacaggctg gcttttttta cgcgttgacc cgtcctgaag agcgttcaca
    15481 tagatcctgc tgatataaaa cacccctgtt ttcccgttta ttcattgatc gaaataagag
    15541 caaaaacatc cacctgacgc ttaaatgaag gtactgcctt aattttctgc agataaaagg
    15601 cgtgacgatg gtcgaaaatg gcactttcgt cagcggggat aatccgttat tgaacaattt
    15661 atcctctgtc catttcacga tgaaaaaaat gtagtttttt caaggtgaag cggtttaaat
    15721 tcgttctcaa attacagtca ggacgcgtat gttgaataat gctatgagcg tagtgatcct
    15781 tgccgcaggc aaaggcacgc gcatgtattc cgatcttccg aaagtgctgc atacccttgc
    15841 cgggaaagcg atggttcagc atgtcattga tgctgcgaat gaattaggcg cagcgcacgt
    15901 tcacctggtg tacggtcacg gcggcgatct gcttaaacag gcgctgaaag acgacaacct
    15961 taactgggtg cttcaggcag agcagctggg tacgggtcat gcaatgcagc aggccgcacc
    16021 tttctttgcc gatgatgaag acattttaat gctctacggc gacgtgccgc tgatctctgt
    16081 cgaaacactc cagcgcctgc gtgatgctaa accccagggt ggcattggtc tgctgacggt
    16141 gaaactggat gatccgaccg gttatggacg tatcacccgt gaaaatggca aagttaccgg
    16201 cattgttgag cacaaagatg ccaccgacga gcagcgtcag attcaggaga tcaacaccgg
    16261 cattctgatt gccaacggcg cagatatgaa acgctggctg gcgaagctga ccaacaataa
    16321 tgctcagggc gaatactaca tcaccgacat tattgcgctg gcgtatcagg aagggcgtga
    16381 aatcgtcgcc gttcatccgc aacgtttaag cgaagtagaa ggtgtgaata accgcctgca
    16441 actctcccgt ctggagcgcg tttaccagtc cgaacaggct gaaaaactgc tgttagcagg
    16501 cgttatgctg cgcgatccgg cgcgttttga tctgcgcggt acgcttactc acgggcgcga
    16561 tgttgaaatt gatactaacg ttatcatcga gggcaacgtg actctcggtc atcgcgtgaa
    16621 aatcggcacc ggttgcgtga ttaaaaacag cgtgattggc gatgattgcg aaatcagtcc
    16681 gtataccgtt gtggaagacg cgaatctggc agcggcctgt actattggcc cgtttgcccg
    16741 tctgcgtcct ggtgctgagt tgctggaagg tgcacacgtt ggtaacttcg ttgagatgaa
    16801 aaaagcgcgt ctgggtaaag gcacgaaagc cgggcatctg acttacctgg gcgatgcgga
    16861 aattggcgat aacgttaaca tcggcgcggg aaccattacc tgcaactacg atggtgcgaa
    16921 taaatttaag accattatcg gcgacgatgt gtttgtcggt tccgacactc agctggtggc
    16981 cccggtaaca gtaggcaaag gcgcgaccat tgctgcgggt acaactgtga cgcgtaatgt
    17041 cggcgaaaat gcattagcta tcagccgtgt gccgcagact cagaaagaag gctggcgtcg
    17101 tccggtaaag aaaaagtaat tctggccggg taatccggtc atctgggatg aggagataac
    17161 ataatctccc tcccacaagc agtaactata aaaataaccc cactctctac aaggctcggg
    17221 gcgcccgaaa aaacgggcat acaggttgac cgacaacgat ataaatcgga atcaaaaact
    17281 atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct tcttgaaggt
    17341 ttacgtcgtc tggaataccg cggatatgac tctgccggtc tggccgttgt tgatgcggaa
    17401 ggtcatatga ctcgcctgcg tcgcctcggt aaagtccaga tgctggctca ggcagcggaa
    17461 gaacatcctc tgcatggcgg caccggtatt gctcacactc gctgggcgac ccacggtgaa
    17521 ccttcagaag ctaacgcgca tccgcatgtt tctgaacaca ttgtggtggt gcataatggc
    17581 atcatcgaaa accatgaacc gctgcgtgaa gagttaaaag cgcgtggcta taccttcgtt
    17641 tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa acaaggcggg
    17701 actctgcgtg aggctgttct gcgtgctatc ccgcagctgc gcggtgcgta cggtacagtg
    17761 atcatggact cccgtcaccc ggataccctg ctagcggcac gttctggtag tccgctggtg
    17821 attggcctgg gtatgggcga aaactttatc gcttctgacc agctggcgct gttgccggtg
    17881 acccgtcgct ttatcttcct tgaagagggc gatattgcgg aaatcactcg ccgttcggta
    17941 aacatcttcg ataaaactgg cgcggaagta aaacgtcagg atatcgaatc caatctgcaa
    18001 tatgacgcgg gcgataaagg tatttaccgt cactacatgc agaaagagat ctacgaacag
    18061 ccgaacgcga taaaaaacac ccttaccggg cgcatcagcc acggtcaggt tgatttaagc
    18121 gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca gatcctcgcc
    18181 tgtggtactt cttataactc cggtatggtt tcccgctact ggtttgaatc gctggcaggt
    18241 attccgtgcg acgtcgaaat cgcctctgaa ttccgctatc gtaaatctgc cgtgcgtcgt
    18301 aacagcctga tgatcacctt gtcacagtct ggcgaaaccg cggataccct ggcgggcctg
    18361 cgtctgtcga aagagctggg ttaccttggt tcgctggcaa tctgtaacgt tccgggttct
    18421 tctctggtgc gcgaatccga tctggcgctg atgaccaacg cgggt
//
LOCUS       DAJVPT010000083        17908 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVPT010000083 DAJVPT010000000
VERSION     DAJVPT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            303..986
                     /locus_tag="OQM47_002792"
     CDS             303..986
                     /locus_tag="OQM47_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1480883.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1030..5692
                     /locus_tag="OQM47_002793"
                     /pseudo
     CDS             1030..5692
                     /locus_tag="OQM47_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6060..7715
                     /gene="lldP"
                     /locus_tag="OQM47_002794"
     CDS             6060..7715
                     /gene="lldP"
                     /locus_tag="OQM47_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1480884.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7715..8491
                     /gene="lldR"
                     /locus_tag="OQM47_002795"
     CDS             7715..8491
                     /gene="lldR"
                     /locus_tag="OQM47_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1480885.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8488..9678
                     /gene="lldD"
                     /locus_tag="OQM47_002796"
     CDS             8488..9678
                     /gene="lldD"
                     /locus_tag="OQM47_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1480886.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9874..10347
                     /gene="trmL"
                     /locus_tag="OQM47_002797"
     CDS             9874..10347
                     /gene="trmL"
                     /locus_tag="OQM47_002797"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1480887.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10400..11221)
                     /gene="cysE"
                     /locus_tag="OQM47_002798"
     CDS             complement(10400..11221)
                     /gene="cysE"
                     /locus_tag="OQM47_002798"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1480888.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11301..12320)
                     /gene="gpsA"
                     /locus_tag="OQM47_002799"
     CDS             complement(11301..12320)
                     /gene="gpsA"
                     /locus_tag="OQM47_002799"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1480889.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12320..12787)
                     /gene="secB"
                     /locus_tag="OQM47_002800"
     CDS             complement(12320..12787)
                     /gene="secB"
                     /locus_tag="OQM47_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1480890.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12850..13101)
                     /gene="grxC"
                     /locus_tag="OQM47_002801"
     CDS             complement(12850..13101)
                     /gene="grxC"
                     /locus_tag="OQM47_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1480891.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13243..13674)
                     /locus_tag="OQM47_002802"
     CDS             complement(13243..13674)
                     /locus_tag="OQM47_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1480892.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13919..15463
                     /gene="gpmM"
                     /locus_tag="OQM47_002803"
                     /gene_synonym="pgmI"
     CDS             13919..15463
                     /gene="gpmM"
                     /locus_tag="OQM47_002803"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1480893.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15473..16756
                     /gene="envC"
                     /locus_tag="OQM47_002804"
     CDS             15473..16756
                     /gene="envC"
                     /locus_tag="OQM47_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1480894.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16760..17719
                     /locus_tag="OQM47_002805"
     CDS             16760..17719
                     /locus_tag="OQM47_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1480895.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17706..17908)
                     /locus_tag="OQM47_002806"
                     /pseudo
     CDS             complement(17706..17908)
                     /locus_tag="OQM47_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4500 a         4639 c         4658 g         4111 t
ORIGIN      
        1 taattgtgaa attttaatca tcatattcac aaaatcaatt atctgtgaaa tttttcccag
       61 acatggtttc tttcatattt gacatttatt aataatcatc attagaatcc acgaaaaata
      121 atatattcat acaaacacag acatcttttg aaacgaatga tacattttta tgatgaatgc
      181 ttaaaacaat atttaatcga cacacatcac tgtcattaat gtaaagcaac atagctcacc
      241 aataatacaa tggacaactc taccggcatt gagcgctggt cggaggaaat aaggattttt
      301 acatgaactt gaagaagatt tttttcagtg ctgtcactgt ttcagttctc tgcgcattaa
      361 cgggatgtga ctacattgaa gaaggcaagc cagaaagtag tttactgaaa caacaagaag
      421 aacataataa taaaattgtg ctacttgaaa aacaacaagc acagctaaag agtcaactcg
      481 aaactattca aaaacaacaa actggcataa taaacagtac aaagacttta actcacgtaa
      541 ttaaatccgt taaagaccaa caaaatactt ttatttttac cgagtttaac ccagcaaaaa
      601 cgaagtattt catcctgaac aacggttcgg tggctttagc gggtcgagtg ttatccattg
      661 acgccacaga gaatggtagt gttattcata tctcactggt caacttatta agtacaccta
      721 tctcaaacat tggttttaat gcgacatggg gtggtgaaaa acctgtcgat gccaaagagt
      781 ttgcccggtg gcaacaatta cttttcaaca catcaatgaa atccacattg aaattattac
      841 caggtcaatg gcaagacatt aatttgaccc tgaagggtgt atcgcccaat aacctgggat
      901 atctgaagtt agccatcaac atggaaaata ttcagttcga caatcttccc tctgctgaaa
      961 atcggcagaa aagaagcaaa aaataaatgc agttagtacg cttataacaa ggaatagcga
     1021 gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact
     1081 gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt
     1141 tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat
     1201 gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg ggttgctata
     1261 ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac tgctgtgggt
     1321 tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt
     1381 ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag aagtatagca
     1441 ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg ttattcaaaa
     1501 gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg agcaaacgcc
     1561 attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt tggcagacaa
     1621 tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc cgctgaaaat
     1681 gcaactgcta ttggcaataa tgcgaaggca aaagggacta atagcatggc aatggggttc
     1741 ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca ggctctggca
     1801 gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc catcgctctg
     1861 ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc cagtaatgca
     1921 actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat taactctgtc
     1981 gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg caatagttca
     2041 ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc
     2101 attaatggtt cacagcttta tgccattagc gactcggtag caaaaaaggc ttggaggagg
     2161 ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg
     2221 tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga
     2281 ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat
     2341 caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta acggttccca
     2401 actgaaagct accaatgacg atgtcgaagc caacaccacc aatatcacca acctgacgga
     2461 ttccgttggt gaccttcagg ctgatgccct gctctggaac gaaactaaaa aggcattcag
     2521 tgcagctcac ggccaggata ccaccagcaa aatcaccaac gttaaagatg ccgacctgac
     2581 ggctgacagc actgatgctg ttaacggctc tcagctgaaa accaccaacg atgctgtggc
     2641 gacgaatacc accaatatcg ccaataacac ttccaatatt gccactaaca ccaccaacat
     2701 ctctaacctg actgagacgg tgactaatct tggtgaggat gcgctgaaat gggataagga
     2761 caatggtgta ttcacggcag ctcatggcac cgagaccacc agcaaaataa ccaacgttaa
     2821 agatggcgac ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac
     2881 caacgatgcc gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa
     2941 tctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg
     3001 tgtcttcact gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg
     3061 cacagtcact gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag
     3121 caatatcgcc acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg
     3181 tccaacctac aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat
     3241 taactcctca tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa
     3301 attcagtgcc aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg
     3361 tgaaatttca gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag
     3421 ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca ccatcactgc
     3481 gccgacgtac accattgcta atgctgatta cgataatgtc ggtgatgccc tgaatgctat
     3541 cgataccact cctgacgacg ctctgctctg ggatgcggac gccggtgaaa atggtgcatt
     3601 tagcgccgct cacggaaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc
     3661 aatctctgct gccagcagcg acgcgattaa cggctcacaa ctctatacca ccaataagta
     3721 catcgctgat gcgctgggtg gtgacgcaga agtcaacgct gacggcacca tcaccgcacc
     3781 gacttacacc attgcgaacg ccgagtacaa caacgtcggt gacgccctgg atgcgcttga
     3841 tgataacgcc ctgctgtggg ataagactgc caatggcggt gctggagcct acaatgccag
     3901 ccatgacggt aaagccagca tcatcactaa tgtcgctaat ggcagtatta gtgaggacag
     3961 taccgatgca gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacac
     4021 ccaaattatc aatcagctcg ctggtaacac cgacgcaacc tatatcgaag aaaatggcgc
     4081 tggtatcaac tacgttcgta ctaacgacaa cgatttagcc tttaacgatg caagcgcctc
     4141 tggtgtcggc gctacagctg taggttataa cgctgtcgcg tctggtgcca gcagcgtagc
     4201 cattggtcag aacagcagca gcaccgttga taccggtatt gcgctgggta gcagctccgt
     4261 ttccagccgt gtgattgcca aaggttctcg tgacactagc gtaacggaaa atggcgtggt
     4321 gattggttat gacaccactg acggcgaact gctaggtgca ttgtcaattg gtgatgacgg
     4381 taaataccgc caaatcatca acgtagctga tggttcagaa gcccatgacg ccgttacggt
     4441 tcgccagttg cagaacgcta ttggagcggt cgccactacg ccaaccaagt actttcacgc
     4501 caactcaacg gcagaagact cactggccgt tggtgaagac tcgctggcaa tgggtgcgaa
     4561 aactgtcgtt aatggtaatg caggtattgg tatcggtttg aacactctgg ttctggctga
     4621 tgcgatcaac ggcattgcta tcggcagtaa cgcacgcgca aaccatgcaa acagtatcgc
     4681 aatgggtaac ggttctcaga ccacccgtgg tgcacagact ggctacaccg cctacaacat
     4741 ggacgcaccg cagaactctg taggtgagtt ttctgtcggt agtgaagacg gtcaacgtca
     4801 gatcacaaac gtcgcagctg gttcagcgga taccgatgcg gttaacgtgg gtcagttgaa
     4861 agtcactgat gagcgcgtag cgcaaaatac ccagagcatt actaacctga acaatcaggt
     4921 cactaatctg gatactcgcg ttactaatat cgaaaacggt attggcgaca ttgtcaccac
     4981 cggtagcacc aagtacttca agaccaacac cgatggcgta gatgccaacg cccagggtaa
     5041 agatagcgtt gctattggtt ctggttccat tgctgccgct gacaacagcg tcgcactggg
     5101 taccggttcc gttgcagagg aagaaaatac aatctctgta ggttcttcta ctaaccaacg
     5161 ccgtatcacc aacgtagctg ccagtgttaa tgccaccgat gcggttaacg tgtcgcagct
     5221 gaaatcttct gaagcaggcg gagtgcgcta cgacaccaaa gctgatggtt ctatcgacta
     5281 tagcaatatc accctcggtg gtggcaacgg cagtacgact cgtatcagca acgtctccgc
     5341 tggcgtcaac aacaacgacg cggtgaacta cgcgcagttg aagcaaagcg cgcaggaaac
     5401 gaagcaatac accgatcagc ggatggttga gatggataac aaactgtcta aaactgaaag
     5461 caagttgagc ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttatac
     5521 accgggtgcc agcatggctt ctattggtgg cggtacttac aacggtgaat cggcagttgc
     5581 tttaggtgta tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac
     5641 caatagccag ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat
     5701 taagttagtg tgactaaggg caaacgccaa attgcctgat gcgttacgcg catcaggcaa
     5761 gataaggcgc tccgggtcaa caatctaagg gcaattctct gatgaggatt gcccttttct
     5821 ttaccagaca tctcccccca caagaattgg ccctaccaat tcttcgctta tctgacctct
     5881 ggttcacaat tccccaatta aaactcacat caatattgcc aatacataac atttagttaa
     5941 ccattcattg tcattatccc tacacaacac aattggcagt gccactttta cacaacgtgt
     6001 gacaaggaga tgagcaacag actcattaca cgatgtgcgt ggactctagg agacctgcaa
     6061 tgaatctctg gcaacaaaac tacgatcccg ccgggaatat ctggctttcc agtctgatag
     6121 catcgcttcc catcctgttt ttcttctttg cgctgattaa gctcaaactg aaaggatacg
     6181 tcgccgcctc atggacggta gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc
     6241 cggtcgctaa cgcgctggcc tcggtggttt atggcttctt ctatggtttg tggcccatcg
     6301 cgtggatcat tattgcagca gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg
     6361 acatcattcg ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa atgctgatcg
     6421 tcggtttctg tttcggcgct ttccttgaag gagccgcagg ctttggcgca ccggtagcaa
     6481 ttaccgccgc attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc
     6541 tgattgttaa caccgcgcca gtggcatttg gcgcgatggg cattccaatc ctggttgccg
     6601 gacaagtaac aggcatcgac agctttgaga ttggccagat ggtggggcgt cagctaccgt
     6661 ttatgaccat tatcgtgctg ttctggatca tggcgattat ggacggctgg cgcggtatca
     6721 aagagacgtg gcctgcggtc gtggttgcag gcggctcgtt tgccatcgct cagtacctta
     6781 gctctaactt cattgggccg gagctgccgg acattatctc ttcgctggta tcactgctct
     6841 gcctgacgct gttcctcaaa cgctggcaac cagttcgcgt attccgcttc ggtgacttgg
     6901 gggcgtcaca ggttgatatg acgctggccc acaccggtta caccgcgggt caggtgttac
     6961 gtgcctggac accgttcctg ttcctgaccg ctaccgtaac actgtggagc atcccgccgt
     7021 ttaaagccct gttcgcatcg ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc
     7081 cgtacctcaa taaactggtt gcccgtatgc caccagtggt cagcgaggct acagcctatg
     7141 ccgccgtgtt taagtttgac tggttctctg ccaccggcac cgccattctg tttgctgcac
     7201 tgctctcgat tgtctggctg aagatgaaac cgtctgacgc tatcagcacc ttcggcagca
     7261 cgctgaaaga actggctcta cccatctact ccatcggcat ggtgctggcg ttcgccttta
     7321 tctcgaacta ttccggactg tcatcaacac tggcactggc actggcgcac accggtcatg
     7381 cattcacctt cttctcgccg ttcctcggct ggctgggggt attcctgacc gggtcggata
     7441 cctcatctaa cgccctgttc gccgcgctgc aagccaccgc agcacaacaa attggcgtct
     7501 ctgatctgtt gctggttgcc gccaatacca ccggtggcgt caccggtaag atgatctccc
     7561 cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag tctgatttgt
     7621 tccgctttac tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg atcaccacgc
     7681 ttcaggctta tgtcttaacg tggatgattc cttaatgatt gttttaccca gacgcctgtc
     7741 agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat
     7801 gaagttgccc gctgagcgcc aactggcgat gcaactcggc gtatcacgta attcactacg
     7861 cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac
     7921 gtttattcgc tggcgtcatg acacatggtc ggagcaaaac atcgtccagc cgctaaaaac
     7981 gctgatggcc gatgatccgg attacagttt cgatattctg gaagcccgct acgccattga
     8041 agccagcacc gcatggcatg cggcaatgcg cgccacacct ggcgacaaag aaaagattca
     8101 gctttgcttt gaagcaacgc taagtgaaga cccggatatc gcctcacaag cggacgttcg
     8161 ttttcatctg gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg
     8221 tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt atctggtgcc
     8281 accggttttt tcacaactga ccgaacaaca tcaggctgtc attgacgcca tttttgctgg
     8341 tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt agttttgttc acaccaccat
     8401 gaaacgattc gatgaagatc aggctcgcca cgcacggatt acccgcctgc ccggtgagca
     8461 taatgagcat tcgagggaga aaaacgcatg attatttccg cagccagcga ttatcgcgcc
     8521 gcagcgcaac gcattctgcc gccgttcctg ttccactata tggatggtgg tgcatattct
     8581 gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag tggcactgcg ccagcgtatt
     8641 ctgaaaaaca tgtccgactt aagcctggaa acgacgctgt ttaatgagaa attgtcgatg
     8701 ccggtagcac tggcaccggt gggtttgtgt ggcatgtatg cgcgtcgtgg cgaagttcag
     8761 gcagccaaag cggcggacgc gcatggcatt ccgtttactc tctcgacggt ttccgtttgc
     8821 ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt ggttccagct ttatgtactg
     8881 cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg
     8941 ctggttttca ccgtggatat gccgacaccg ggcgcacgct accgtgatgc gcattcaggt
     9001 atgagcggcc caaatgcggc aatgcgccgc tacttgcagg cggtgacgca tccgcaatgg
     9061 gcgtgggatg tgggcctgaa cggtcgtcca catgatttag gtaatatctc agcttatctc
     9121 ggcaaaccga ccggactgga agattacatc ggctggctgg ggaataactt cgatccgtcc
     9181 atctcatgga aagaccttga gtggatccgc gatttctggg atggcccgat ggtgatcaaa
     9241 gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga tggaattgtg
     9301 gtttctaacc acggtggccg ccagctggac ggtgtactct cttccgctcg tgcgttgccc
     9361 gctattgcag atgcggtgaa aggtgatatc gccattctgg cggatagcgg aattcgtaac
     9421 gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct gggtcgtgct
     9481 ttcctgtatg cgctggcaac agcgggccag gcgggtgtag ctaacctgct aaatctgatc
     9541 gaaaaagaga tgaaagtggc gatgacgctg actggcgcga aatcgatcag tgaaattacg
     9601 caagattcgc tggtgcaggg gctgggtaaa gagttgccta cggcactggc tccgatggcg
     9661 aaagggaatg cggcatagtc gtttgcgccc cctcacccta accctctccc tcagggagag
     9721 gggaccgttc ggcgctgtat gtactccctc actctgaaac gacaccgcac tctttttttc
     9781 tccctcgccc ctccggggag ggccggggtg aggggaaaag gccgcacggt tcaccaattt
     9841 tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact ttacgaacca
     9901 gaaattccgc caaatactgg caacatcatc cgtctttgcg ctaataccgg ctttcgtcta
     9961 catatcatcg aaccgatggg atttgcctgg gacgacaagc gcctgcgccg cgcggggctg
    10021 gactatcacg agtttaccgc cgttacgcgt catcatgact atcgcgcgtt cctcgaagca
    10081 gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca cagcgccgta
    10141 agctatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct gccagcgagc
    10201 attcttgatg ccctgcccgc tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc
    10261 agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg aagcctggcg gcagctggga
    10321 tatccgggag cgttgttgag agattagtta ctgtatgccg gatagatgat tacatcgcat
    10381 ccggcacgat cacaggacat tagatcccat ccccatactc aaatgtatgg ttaataccgt
    10441 tgaaatgctg gtccatatcc attgatggct tatcgctgtc tggtttaccg acaatacgag
    10501 ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc
    10561 caatcttcgc gccgcgacca acttcaatat tgccgaggat tttcgcgccc gcgccaatca
    10621 tcacaccttc acgaattttc gggtgacggt caccaccaga tttacccgta ccgccaagcg
    10681 tcacagattg cagaatcgat acgtcgtttt caattaccgc cgtttcaccc acaacgatgc
    10741 ccgtggcgtg gtcgagcata atgccacggc caatttttgc tgccgggtga atatcgacct
    10801 ggaacgtcac agaaacctgg ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc
    10861 acaaccagtg accgatgcga taggcctgca aggcatgaaa acccttcagg tataacaacg
    10921 gggttgagta tttatcgact gccgggtcgc gggtacgcac cgcctgaata tcacaggccg
    10981 cagaggcgat catttccggg tcagcggcgt aggcttcttc aaccacttca cggatagcaa
    11041 tagcaggcat aattggcgat gacagcttgt tcgccagcat gtagctcagt gcactgccaa
    11101 ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc tcacagtccg
    11161 ccagcgttct ggcttcggct ttaatattgt tccagacaat ttccagttct tcacacgaca
    11221 ttgcttactc cacacgatga gataatgacc ggcccgttct gcgcgggccg ggtcatagcg
    11281 gtaacaaagg ttccctgggg ttagtgactg ctgcgctcgt ccttgcgtgc acgacctaat
    11341 aatgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata aatttcctcg
    11401 gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg
    11461 tagccttcca ccacctgacc aatcttctcc tgcgcgcttt gtacatccat gccctgaccg
    11521 agcatcatac caaaacggcg gttacgcgac tggttgtcgg tacaggtaag caccagatcg
    11581 cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc accaagacgc
    11641 gacatttcag ccagcccacg ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata
    11701 ccgtcagaca tccccgcgcc aatggcaatg acgtttttca ccgcgccgcc aagctgcacg
    11761 ccaatgaaat ccgggttgct gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg
    11821 agatcatcgg caaaggtctg atcggtcgag gccagcgaaa tagctgtcgg taaacctgcc
    11881 gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg atcgcctaag
    11941 gcctcacgcg ccacgtcctg taacagacgt ccggtttccg cttccagccc tttggtcgcc
    12001 cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag
    12061 acatggctgg gtacgacgac gagaatatta cgactggctg ccagcgcagt ggcgagatcg
    12121 ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc gttacagcgg
    12181 tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac ctcgtggcca
    12241 tttcttgcca gggtaatggc aagagcggtg ccgtacgagc cggcaccgat cacagtcatt
    12301 gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg ccagcctgct
    12361 gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc agttgcggga
    12421 atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac agaatgttcg
    12481 ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg atggagaaaa
    12541 taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccaaggag gccgttacgg
    12601 taacacgcag taccacttcg tatacgtcat ctgccagttg ggaagatgcc gtatccagat
    12661 caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga
    12721 aagagatatc cttggtataa atacgttgga tctggaaagt catttcagtg ttgttttgtt
    12781 ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg taataccaac
    12841 gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg catacaagtc
    12901 atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg tgcaaccgct
    12961 gcgtttgatc atctcttcac gcttggcggc attgccatcg atcggcaact cctggaaact
    13021 cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg tttctttggt
    13081 atagatttca acattggcca taatttagct cctgtttttt taccctgaaa tttcatgttg
    13141 cagggcggct gcaaaatgga gtgtccccag aagcttataa caataagtcg ctgggggcct
    13201 aattgcagct aacggcctgc atcatgaaag acgacaggta aattatttgc cgcgcaccag
    13261 aggcaagttt tcgccagccc agccagcaac gccctctttc agcacgaata cttgcgcaaa
    13321 accagctttc gtcagcgcgt ttgcaggctc ctggcactgc atgccagaac cgtctaccac
    13381 gataaccgat ttgtctttgt gcttctcaag ctcaccaaca ttgttggctt tgatttcgct
    13441 cggcaacaga ttgatagaac ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa
    13501 atccacaacc acagcgtctt ctttgttgat cagacgcgta gcttcaccac gagtaatcac
    13561 cttcactttc gaggtcaggc tcttaaacgt agtcacaaga accgccacca gtaacgcgat
    13621 ccaggcgata ctcagtatgg gatgacggcc aacaaattgc ataatttctt gcatgggggg
    13681 taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg
    13741 caaatacagc cagcgcgtta actggaatgc aattttgcgg ggcacgacga aaaaaaagcg
    13801 caaatctggc atcggcttgc cgcgagcggt cgtatatttt gatcttcaga ggctatttta
    13861 tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat
    13921 gtcggtttct aaaaaaccta tggtactggt gattctggat ggctatggct atcgtgaaga
    13981 acagcaggat aacgccattt ttagtgctaa aaccccggta atggatgcac tgtgggccaa
    14041 tcgtccgcat accctgatcg acgcttccgg tctggaagtc ggtctgcctg accgtcagat
    14101 gggtaactcc gaagtaggcc acgttaacct gggtgccggc cgcatcgtgt atcaggacct
    14161 gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt gctaatccag tgctggctgg
    14221 cgcggtacat aaagcgaaaa acgcaggtaa agcagtacac attatgggtc tgctctccgc
    14281 aggcggcgta cacagccacg aagatcacat catggcgatg gtagaactgg cagctgaacg
    14341 cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag
    14401 tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct gcgctgggta aaggccgcgt
    14461 agcgtccatc attggtcgtt actacgcgat ggaccgcgat aaccgttggg atcgcgttga
    14521 aaaagcctat gacctgctga ctctggcgca gggtgagttc caggccgata ctgccgttgc
    14581 tggtttgcag gcagcttatg ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat
    14641 tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc gatgcgctga ttttcatgaa
    14701 cttccgtgct gaccgcgcgc gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg
    14761 cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga
    14821 catcaaaact gcggttgctt acccacccgc ttccctggtt aacaccttcg gcgagtggat
    14881 ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc gaaaaatatg cccacgttac
    14941 tttcttcttc aacggtggcg tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa
    15001 ctcaccgaaa gtggctacct acgatctgca accggaaatg agctccgcag agctgaccga
    15061 aaaactggtt gcggccatca agagcggcaa atacgacacc attatctgta actatccgaa
    15121 cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact
    15181 ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat
    15241 caccgctgac cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc aggcacacac
    15301 ggcacacacc aacctgccag ttccgctgat ttacgttggt gataagaacg tgaaagcggt
    15361 tgcaggcggc aaactttctg acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat
    15421 cccgcaagag atgactggta agccgctgtt catcgtggaa taatccctcc ccatgagggg
    15481 aaaggcgatt aataccatga cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat
    15541 catctacgcc agcgttctta gcgctggcgt attgttgtgc gccttttccg cccacgcgga
    15601 tgagcgtgac caactcaaat ctattcaggc cgatatcgcc gcaaaagagc gcgcggtacg
    15661 ccaaaagcaa caacaacgcg caagcctgct cgcacaattg aaaaagcagg aagaagcgat
    15721 ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca
    15781 gattgatgag atgaacgcgt cgattgccaa actggagcag caaaaagccg cccaggagcg
    15841 cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc gagcataccg gtattcagct
    15901 aattctcagc ggtgaagaaa gccagcgtgg acagcgttta caggcttatt tcggctatct
    15961 caaccaggcg cgacaagaaa ccattgccca gttgaagcaa acgcgtgaag aagtcaccat
    16021 gcagcatgct gaactggaag agaaacaaag cgagcaacaa acgctgttat atgaacagcg
    16081 cgcccaacag gcgaaactga ctcaggctct gaacgagcgt aaaaagacgc tggcggggct
    16141 ggaatcttcc atccagcaag gtcagcagca gctaagtgaa ctgcgtgcca acgaatcccg
    16201 tctgcgtaac agcattgccc gtgctgaagc cgcggcgaaa gcgcgtgctg aacgagaagc
    16261 gcgtgaggcc caggcggttc gcgagcgcca gaaagaagcg acgcgcaaag gcaccaccta
    16321 caaaccgacc gaaagcgaaa aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg
    16381 tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat cgctatggcg agcagctaca
    16441 gggtgaatta cgctggaaag ggatggttat cggtgcctct gaaggtactg aagttaaagc
    16501 aattgccgac ggtcgagtga ttctggctga ctggctgcaa ggttacggtc tggtggtggt
    16561 ggttgagcac ggtaaaggcg acatgagtct ttacggctat aatcagagcg cactggtgag
    16621 cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca gtggcggtca
    16681 gggtcggcct tcactctatt tcgaaattcg ccgccagggt caggcggtca atccacagcc
    16741 gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct
    16801 gttggcgctc tcctcccccg tacttgctgg caaacttgcc atcgtcattg atgattttgg
    16861 gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct tccgctatct ccgtcgctgt
    16921 attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcgcataaca gcggtcacga
    16981 agtgttgatt catctcccga tggcaccgtt gagtaaacag ccgctggaga aaaatacgct
    17041 acgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg tcaataacgt
    17101 gccctatgcc gtggggatca acaaccacat gggcagcaag atgacctcta acctgtttgg
    17161 tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt tacttcctcg acagcgtaac
    17221 catcggtaat acccaggcga tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg
    17281 gaaggtgttc ctcgacgatt cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc
    17341 aattgatctg gcgcgtcgca acggttcaac cattgccatt gggcatcctc acccttcaac
    17401 ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc tggtgaaagc
    17461 cagcagcttg ctgaatgaac cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc
    17521 tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga
    17581 acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc agagcacgct
    17641 gatcgtttac ttccagcatc agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt
    17701 taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg
    17761 cgccattgcc ataagcggaa agaccacagt aacgcctggt aaccgacctt cacgctgcgc
    17821 acattggtaa tcaggcgctt atacataccc ggaagtaaat atctcggcaa tcatccgttg
    17881 gcgggtaaga atatccggct ctttgcgc
//
LOCUS       DAJVPT010000084        17482 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVPT010000084 DAJVPT010000000
VERSION     DAJVPT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..338
                     /locus_tag="OQM47_002807"
     CDS             <1..338
                     /locus_tag="OQM47_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1480896.1"
                     /translation="GKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGND
                     KAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLS
                     SLVMRVAQD"
     gene            419..1093
                     /locus_tag="OQM47_002808"
     CDS             419..1093
                     /locus_tag="OQM47_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1480897.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1109..3589
                     /locus_tag="OQM47_002809"
     CDS             1109..3589
                     /locus_tag="OQM47_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1480898.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3605..4638
                     /locus_tag="OQM47_002810"
                     /pseudo
     CDS             3605..4638
                     /locus_tag="OQM47_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4720..5058)
                     /gene="rcnB"
                     /locus_tag="OQM47_002811"
     CDS             complement(4720..5058)
                     /gene="rcnB"
                     /locus_tag="OQM47_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1480899.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5277..6098)
                     /gene="rcnA"
                     /locus_tag="OQM47_002812"
     CDS             complement(5277..6098)
                     /gene="rcnA"
                     /locus_tag="OQM47_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1480900.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRGERNWLENMHGHDYEHHHHHHDHEHHHDQGHHHHHEHGEYQDAHARAHANDI
                     KRRFDGREVINWQILLFGLTGGFIPCPAAITVLLICIQLKALTLGATLVVSFSIGLAL
                     TLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6219..6491
                     /gene="rcnR"
                     /locus_tag="OQM47_002813"
     CDS             6219..6491
                     /gene="rcnR"
                     /locus_tag="OQM47_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1480901.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6583..6679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6714..7502
                     /gene="thiM"
                     /locus_tag="OQM47_002814"
     CDS             6714..7502
                     /gene="thiM"
                     /locus_tag="OQM47_002814"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1480902.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7499..8299
                     /gene="thiD"
                     /locus_tag="OQM47_002815"
     CDS             7499..8299
                     /gene="thiD"
                     /locus_tag="OQM47_002815"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1480903.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8364..9185
                     /locus_tag="OQM47_002816"
     CDS             8364..9185
                     /locus_tag="OQM47_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1480904.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9233..9979
                     /locus_tag="OQM47_002817"
     CDS             9233..9979
                     /locus_tag="OQM47_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1480905.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9953..10918)
                     /locus_tag="OQM47_002818"
     CDS             complement(9953..10918)
                     /locus_tag="OQM47_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCS1480906.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10915..11918)
                     /locus_tag="OQM47_002819"
                     /pseudo
     CDS             complement(10915..11918)
                     /locus_tag="OQM47_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            11958..13010
                     /gene="fbaB"
                     /locus_tag="OQM47_002820"
     CDS             11958..13010
                     /gene="fbaB"
                     /locus_tag="OQM47_002820"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1480907.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13318..14172
                     /gene="gatY"
                     /locus_tag="OQM47_002821"
     CDS             13318..14172
                     /gene="gatY"
                     /locus_tag="OQM47_002821"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1480908.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14201..15463
                     /gene="gatZ"
                     /locus_tag="OQM47_002822"
     CDS             14201..15463
                     /gene="gatZ"
                     /locus_tag="OQM47_002822"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1480909.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15473..15925
                     /gene="gatA"
                     /locus_tag="OQM47_002823"
     CDS             15473..15925
                     /gene="gatA"
                     /locus_tag="OQM47_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1480910.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15956..16240
                     /gene="gatB"
                     /locus_tag="OQM47_002824"
     CDS             15956..16240
                     /gene="gatB"
                     /locus_tag="OQM47_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1480911.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16244..>17482
                     /locus_tag="OQM47_002825"
     CDS             16244..>17482
                     /locus_tag="OQM47_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1480912.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFS"
BASE COUNT         4547 a         4015 c         4472 g         4448 t
ORIGIN      
        1 aaggtaaagc taaatattct acctatgatg atgcagtcgg tgtaaccagc agcatgttaa
       61 aaattagctg cccgaaagaa gttgctggtg taaaactctc gttgatcaca aacgacaaaa
      121 taaccggtaa cgataaggcg atagccagta gcaacgatac cgtaggtgat aacagcgatg
      181 tcctagatgt ttctgcacct tttaacattg agagttataa aacagcggaa ggtcaatatg
      241 ctattccgtt taaagcaaaa tacctgaaac tgacagataa ctcagtgcaa tcaggtgatg
      301 tgttatcttc tctggttatg cgtgtggcgc aggattaata aataaaattg caagtttctc
      361 caggtaataa acatggccgc tatccccatg gcggcttttt aatttaagag gcattaagat
      421 gaaaggatta ttatctttac tcattttttc tatggtcctt cctgcacatg ccggaattgt
      481 tatctacggg acgcgcatta tttacccggc agaaaataaa gaagttatgg tgcagttgat
      541 gaaccaggga aatcgttctt cgctgctgca ggcgtggatt gatgatggcg atacgtcgtt
      601 accaccagaa aaaattcagg ttcctttcat gttaacgcca ccagtggcaa aaataggggc
      661 aaattccggg cagcaagtaa aaatcaaaat tatgccgaat aaactgccca ctaataaaga
      721 aagcattttt tatctgaatg ttctgtatat tccaccaaat agtccagagc aagaaggcaa
      781 gaatgcactg aagtttgcga tgcaaaacag aattaagttg ttttaccggc cagcgggtat
      841 tgctccggta aataaagcga cctttaaaaa gttactggta aatcgcagtg gcaatggatt
      901 ggtgataaaa aatgactcag ctaattgggt gacgatttcg gatgtcaaag ctaataatgt
      961 taaagtcaat tatgaaacta ttatgattgc ccccttagaa agtcagagtg ttaatatcaa
     1021 aagtaataat gcaaataact ggtatctgac cattatcaat gaccacggca actatattag
     1081 tgacaaaatt taattgcagg agctgcctat gttgagaatg accccacttg catcagcaat
     1141 cgtagcgtta ttgctcggca ttgaagctta tgcagctgaa gaaacctttg atacccattt
     1201 tatgataggt ggaatgaaag accagcaggt tgcaaatatt cgtcttgatg ataatcaacc
     1261 cttaccgggg cagtatgaca tcgatattta tgtcaataag caatggcgcg ggaaatatga
     1321 gattattgtt aaagacaacc cgcaagaaac atgtttatca agagaagtta tcaagcggtt
     1381 aggcattaat agcgataact tcgccagcgg taagcaatgt ttaacatttg agcaacttgt
     1441 tcagggtggg agctatagct gggatatcgg ggtttttcgt ctcgatttca gtgtcccgca
     1501 ggcttgggtg gaagaactgg aaagtggcta tgttccaccg gaaaactggg agcggggtat
     1561 taatgcgttt tatacttctt attatgtgag tcagtattac agcgactata aagcgtcggg
     1621 taacaacaag agtacatatg tacgttttaa cagcgggtta aatttactgg agtggcaact
     1681 gcattctgat gccagtttca gtaaaacaaa taacaatcca ggggtgtgga aaagcaatac
     1741 cctgtatctg gaacgtggat ttgcccaatt tctcggcacg cttcgcgtgg gtgatatgta
     1801 cacatcaagc gatatttttg attctgttcg cttcagcggt gtgcggttgt ttcgtgatat
     1861 gcagatgttg cctaactcga aacaaaattt tacgccacgg gtgcagggga ttgctcagag
     1921 taacgcgctg gtaactattg aacagaatgg ttttgtggtt tatcagaaag aggttcctcc
     1981 tggcccgttt gcgattacag atttgcagtt ggccggtggt ggagcagatc ttgatgttag
     2041 cgtgaaagag gcggacggct cggtaaccac ctatctggtg ccttatgcag cggtgccaaa
     2101 tatgctgcaa cccggcgtgt cgaaatatga ttttgcggcg ggccgtagcc atattgaagg
     2161 ggcgagcaag caaagtgatt ttgtccaggc aggttatcag tatggtttta ataacttatt
     2221 gacgctgtat ggcggcacga tggttgctaa taattactat gcgtttaccc tcggaacggg
     2281 ttggaacacg cgcattggcg caatttccgt cgatgccacg aagtcgcata gtaaacaaga
     2341 caacggcgat gtgtttgacg ggcaaagtta tcaaattgcc tacaacaaat ttgtgagcca
     2401 aacgtcgacg cgttttggtc tggcggcctg gcgttattcg tcgcgtgatt accggacatt
     2461 taacgatcac gtttgggcaa acaataaaga taattatcgc cgtgatgaaa acgatatcta
     2521 tgacattgcc gattattacc agaacgattt tggccgtaaa aatagctttt ctgccaatat
     2581 gagccagtca ttgccagaag gctggggttc tgtgtcctta agtacgttat ggcgagatta
     2641 ctgggggcgt agcggcagca gtaaggatta tcagttgagt tattccaata actggcggcg
     2701 gataagctat accctcgcgg caagccaggc gtatggcgag aatcatcatg aagagaaacg
     2761 ttttaatatt tttatatcaa ttccctgtga ttggggtgat gacgttacga cgcctcgtcg
     2821 gcaaatatat atgtctaact caacgacgtt tgatgatcag ggttttgcct caaataatac
     2881 gggattatca ggaacagtag ggagtcggga tcagttcaat tatggtgtca acctgagtca
     2941 tcaacatcag ggaaatgaaa cgacagctgg ggcgaatttg acctggaacg cgccggttgc
     3001 gacagtgaat ggcagttata gtcagtcgag tacttatcga cagactggag ccagtgtttc
     3061 agggggcatt gtcgcctggt cgggtggcgt taatctggcg aaccgtcttt ccgaaacgtt
     3121 tgctgtgatg aatgcgccag gaattaaaga tgcttatgtc aatgggcaaa aatatcgcac
     3181 aacaaaccgt aatggagtgg tggtatacga cggaatgaca ccttatcggg aaaatcacct
     3241 gatgctggat gtgtcgcaaa gcgatagcga agcagaatta cgtggcaacc ggaaaattgc
     3301 cgccccttat cgcggcgcgg ttgtactggt taattttgat accgatcagc gcaagccctg
     3361 gtttataaaa gcgttaagag cggatggtca accattaacg tttggttatg aagtcaatga
     3421 tatccatggt cataatattg gcgttgtcgg ccagggaagc cagttattta ttcgcaccaa
     3481 tgaaataccg ccatcggtta atgtagcaat tgataagcaa caaggacttt catgcacaat
     3541 caccttcggt aaagagattg atgaaagtag aaattatatt tgccagtaat agtcaggtgg
     3601 ttctatggaa attcgcataa tgctatttat attaatgatg atggttatgc ctgtgagcta
     3661 tgcggcatgt tatagtgagt tatctgttca gcacaatttg gttgttcagg ggaattttgc
     3721 acttactcaa acacaaatag cgacatatga gcataatttt aatgattcgt catgcgtgag
     3781 tacaaatact atcaccccta tgagcccatc ggatattatt gttggacttt ataacgatac
     3841 cattaaatta aatttacatt ttgaatggac caataaaaac aacatcacgt tgtcaaataa
     3901 tcagagcagt ttcaccagtg gttattcggt aacggtgaca cctgcggcca gtaatgcaaa
     3961 agtgaatatt tctgcggggg gcggtggttc agtgatgatt aatggtgttg cgacattatc
     4021 cagtgcttca tcatcgacac gcgggagtgc cgcagtacaa tttctactgt gtttattagg
     4081 tggcaagtca tggggtgcat gtgtaaatag ctacagaaat gcattggcac aaaatgcagg
     4141 tgtctattcc ttcaatctga cattgtcata caacccgata accacaacct gcaaacagga
     4201 cgatttatta attactttag acagtattcc cgttttacaa ttaccagcca caggtaacaa
     4261 agcaacaata aatagtaaaa aaagggatat tattctgcgt tgtaaaaatt tattaggtca
     4321 acaaaatcaa acatcacgga aaatgcaggt gtatttatca agttctgact tgttaaccaa
     4381 cagcaacaca atactgaaag gtgcggaaga taatgcgtag gatttattct tgaaagtaat
     4441 ggttcgccag tcacactttt aaatatcact aacagcagta aaggatacac aaatttaaaa
     4501 gaaattgcgg caaagtcaaa acttacagat acaacggttt caattccgat aacagccagt
     4561 tactacgtct acgatacaaa caaagttaaa tctggcgcac tggaggcaac cgcattaatc
     4621 aacgtgaaat acgactaata acaagcaaaa cgagcaagtg gctaataata aaaaataaca
     4681 aggtgtctgc atttccccct catgatgagg gggctttttt tagcgatgat aaaaaatctc
     4741 accgtcgtag gctttaatga ttttaccgtc ggtgtcgctg atcaacacgt acgcgccacc
     4801 catataggtc cagtgcgtcc cggcatcagg cgcgggcagg ttacgtaact gccactgctt
     4861 aatgttgtac tcatcggtgc ggtacatctc tgggacggta tcacctggtt tgaaatgctt
     4921 aaagtcagca atgaaactac ttaactcata cttagaaatt cccgaggtat tggtcgaacc
     4981 cgcggtggct ggtgcggccc aggcggcact ggtaaccagc aaaagcacac ccagcaacat
     5041 cttattttta atggtcataa actctccact ttggggatct gtaatatcag tcgcatatct
     5101 ttcctgtcat acaatataac tgacaagcga tattttgtta gtgatcccat gatttcgtaa
     5161 aaagtgtaaa tgaaaggatg ccctaaaagc atccttagcg agcagagagt cagcggaaat
     5221 agctgattta agaatcatcc tgggctgaag ataacatgca agcctaaagg attgagttat
     5281 cgcattatgc ccatgaagcc atgtacgccc atatacacac cgactaagcc aatcaacaga
     5341 ctggaaaaat agggggcgcg tttagcgaga gtgttaaatc cgctccagcg ttttgcgacc
     5401 tgctgaacgc tgattgctgc gccaacgcct acggtgacaa gcgttaacgc caggccaatg
     5461 ctgaaactga cgaccagtgt tgcgcccagt gtcagggctt tcaactgaat gcatatcaac
     5521 agcacggtaa ttgctgccgg gcaggggata aagccaccgg ttaagccaaa taacaaaatt
     5581 tgccagttga tgacctctct accatcaaag cgtcgtttaa tgtcattggc atgggctcgt
     5641 gcatgggcat cctgatactc gccatgttcg tgatggtgat gatgtccttg gtcgtggtga
     5701 tgttcgtgat cgtgatgatg gtgatgatgc tcataatcat gcccgtgcat attctccagc
     5761 cagttgcgtt cgccgcgcca gaacatccag aacgcggtgc caataatgat cactgcggaa
     5821 atcagctgga gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg
     5881 ccaaaggcaa tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc
     5941 accgcctgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag
     6001 tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag
     6061 gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat
     6121 tcggcaatct gattctactc ccccccagta cctgatacta ccccccagta gattaatagt
     6181 gctatgattt ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata
     6241 aacagaaact gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa
     6301 tgctcgacga gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag
     6361 cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc
     6421 agggggatga gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat
     6481 atatcaaata atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggg
     6541 gcgctggaca ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc
     6601 ccttctgcgt gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg
     6661 gaagtcacgg accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag
     6721 tcgacctgct gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc
     6781 ctcttgtgca ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg
     6841 cgctcggtgc atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta
     6901 tcgccagtgc cttgttgatt aacgttggca cactgacgca gccacgcgca caggcgatgc
     6961 gtgctgccgt tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg
     7021 ttggtgcgct cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag
     7081 cgatacgtgg taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg
     7141 gagtggatac cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg
     7201 aaactggcgc aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta
     7261 tcattggtat tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat
     7321 tatcggcggt tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat
     7381 ctgcctgtca ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag
     7441 gcagttttgt tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat
     7501 gaaacgaatt aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca
     7561 ggccgatctt aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact
     7621 ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc
     7681 cgcccagctc gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt
     7741 ggcggaaacc gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa
     7801 cgtggtactc gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc
     7861 ggttgctacg ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc
     7921 cgaagctgcc gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca
     7981 agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga
     8041 tgatgagcaa agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc
     8101 gcgcattatg accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc
     8161 actacgcccg cgccatacaa actgggctga caccgtacag gaggcaaaaa tctggctttc
     8221 atcggcgtta gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca
     8281 cttccacgcc tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc
     8341 actgacattc agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg
     8401 ctctattatg cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga
     8461 attttttcta ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc
     8521 cggttaaatc tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt
     8581 cgcgctggca ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc
     8641 gcttacagtt tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt
     8701 cgcgtaactg gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct
     8761 ccccgtcggt atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac
     8821 aaggcgattt ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat
     8881 tacgcaagcg ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga
     8941 ttatttactc aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc
     9001 cgtggtgggt ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct
     9061 tctggcagca ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg
     9121 tgtttaatgg cacggggatg agttgcaggc attcgttgat gggattaaag aaacgcctta
     9181 aataagaagc agtgatatac tcccaccaga ctccctggca ggaaaaagac aaatggaaca
     9241 agcgcatacc cagctgattg cccaactgaa cgagcgtatt ttagcggcgg ataacacgcc
     9301 gctttatatt aagtttgccg aaacggtaaa aaatgccgtg cgcagcgggg tgctggagca
     9361 tggcaatatt ttgcccggtg agcgtgattt aagtcagtta accggcgttt cgcgcattac
     9421 ggtgcgcaag gcgatgcagg cgctggaaga agagggcgtg gtgacgcgtt cgcgcggtta
     9481 cggcacgcag atcaacaaca tcttcgaata ctcgttgaaa gaagcgagag gtttttctca
     9541 gcaggtggta ttgcgcggga aaaagcccga tacgctatgg gttaataagc gtgtcgtgaa
     9601 atgtcccgaa gaagttgcgc agcagctggc ggtcgaagcg ggaagtgatg tctttttgct
     9661 taagcgtatt cgctatgtcg atgaagaagc ggtatcgatt gaggaatcgt gggttccggc
     9721 gcatttaatt catgatgttg atgccatcgg aatttctctt tatgactatt tccgcagcca
     9781 gcacatttac ccacaacgta cgcgttcccg cgttagcgcc cggatgccgg atgccgagtt
     9841 tcagtcacat attcagttag atagcaaaat accggtgctg gtgatcaagc aagtggcgct
     9901 tgaccaacag caacggccta ttgagtacag cattagccac tgtcgcagcg atctatacgt
     9961 ttttgtgtgc gaggagtagt tcctcgcgcg ttggcgcaca atcaccgccc cgatgcccga
    10021 caacccacga cgccactgca ttgcccagta atacggcatc cgccagtggc agaccagagg
    10081 ccagcccggc aagtacgcca cctgcatgac tgtcgcccgc cccaatggtg tctacaactt
    10141 gcgttggaaa tgccggaatg cagccagaag cgtcgttgct gaaataccat gcgccttctt
    10201 tatcgagacg aacgatcaac ggcgcggcaa atttctcctg ccattgcttg ccaagtgttg
    10261 ttatctctgc ggataaagca aaacgttcgg cggcaatctc agcctcttga cgattgagtg
    10321 acactaaagg tcgacaggcc atgatccgcg ccagtaatgc gtccgggata tcgccaatac
    10381 gtgggcaaaa atcgataaac ggcgtcacgt cttgcagctt ttccagccat tccactaaca
    10441 attcgccgca gggcgaggcc agttgataac cggaaaaata gagcaggttg ccaggcgcaa
    10501 cggttaatcg tgccaaccat tggcgattcc attgattttc cacaccgctg aatgacataa
    10561 aagtgcgttc gccatccggc tcaaccagtg ccagacacca gccgttatcg ctttcggcgt
    10621 tatcgatcag gctgattaac ccctctttcg ccatccggtt gcgaataatc tccgcccaca
    10681 cgccctgacc gagcggcaag gcattacccg cttcgatgcc gaggcgcttt aacgccacgg
    10741 caatattcag tgcgcagccg ccaacgttaa cgctctgctg tttcagttcg atatcgcacc
    10801 cacgccaggg gagggcataa gcgtcggcga tcacgtcaat gaccgccgcg ccgacgacca
    10861 tcaccggctg acgcgtggtt aattcaggca gcagtgtgtg taatcgagcg ccgctcatac
    10921 cgcctcccgt tgttgacgat atttcgccag cgctgtggca tagcggttga aatcaagctg
    10981 atttaccgca tccagctccg cctttaacgc aggatcgaca gcgttaatgc catgcaacgc
    11041 gccgcaaatc gccgtcgcca tagcacctat ggtgtctgtg tcgccgccaa gattggcgca
    11101 caggacggcg cagcggtttg ggtcggtttg tgccagttca actagcgcaa tggcgcacgg
    11161 aacggactca atagtgctgg tgcctgcgcc aacgacctgg taaagctgtt cgctggcgga
    11221 ttcggtgccg tcggcattac gcacaatttt cagcgcaatc tccagacgtg ccgccagtga
    11281 ggcgctgaac gtggtggtgc gtttttgctg tgcatgtcgc gcaattgaag gcaatgaatc
    11341 aacaatcgct gaccagcgtt ctccgtcaat ggcacgagaa atcgcccatg cgatgactac
    11401 cgcgcctgca accgccagat cggatttatg ggtcgggctg gacgccagcg ccacatcatc
    11461 aataaaggaa tcaacatcac gcgctggaag caaacaacct aatggcgaga cgcgcatcgc
    11521 tgcgccgttg gttacgccgt tgttttccag ttcagcaacg ggtttaccgt cgcgaatggc
    11581 gttaagcgca atcttcgagg tcggacctag tacgtttttg ttaaaggcgt cgaaacgcaa
    11641 cgcccagtcg agaatattac gcccaatcag attcggatcg atcttgcctt cacattccag
    11701 taacgcatcc gccagacaca ggccatcgag gtatcgtcgg tgaattcggc gcggttaaaa
    11761 taacaggccg cgttattctc ctttggtcca ggcagaaaac ggtcaatcca gccaaagtgt
    11821 gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc
    11881 tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct tgaatcggaa
    11941 tttacgggag catagtaatg acagatattg cgcagttgct tggcaaagac gccgacaacc
    12001 ttttacagca ccgttgtatg actattcctt ctgaccagct ttatctcccc ggacatgact
    12061 acgtagaccg cgtgatgatt gacaataatc gcccgccagc ggtgttacgt aatatgcaga
    12121 cgttgtacaa caccgggcgt ctggctggca caggatatct ttctattctg ccggttgacc
    12181 agggcgttga gcactctgcc ggagcttcat ttgctgctaa cccgctctac tttgacccga
    12241 aaaacattgt tgaactggcg atcgaagcgg gctgtaactg tgtggcatca acttacggcg
    12301 tgttggtgtc ggtatcgcgg cgctatgcgc atcgcattcc atttctcgtc aaacttaatc
    12361 acaacgagac gctaagttac ccgaatacct acgatcaaac gctgtatgcc agcgtggagc
    12421 aggccttcaa catgggcgcg gtggcggttg gtgcgactat ctattttggc tcggaagagt
    12481 cacgtcgcca gattgaagaa atttctgcgg cctttgaacg cgcgcacgag ctgggcatgg
    12541 tgacagtgct gtgggcctat ttgcgaaact ccgcctttaa gaaagatggc gttgattacc
    12601 atgtttccgc cgacctgact ggtcaggcga accatctggc ggcaaccata ggtgcagata
    12661 tcgtcaaaca aaaaatggcg gaaaataacg gcggctataa agcgattaat tacggttata
    12721 ccgacgatcg cgtgtacagc aagttaacca gcgaaaaccc gattgatctg gtgcgttatc
    12781 agttagctaa ctgctatatg ggccgggctg ggttgataaa ctccggcggt gctgcaggcg
    12841 gtgaaactga cctcagcgat gcagtgcgta ctgcggttat caacaaacgc gcaggcggaa
    12901 tggggctgat tcttggacgt aaggcgttca agaaatcgat ggctgacggc gtgaaactga
    12961 ttaatgccgt gcaggacgtt tatctcgata gcaaaattac tattgcctga tacgcctcat
    13021 acgaacagca ccaccccgta ggacggataa gacgtttaca ctgcatccgg caacctatgc
    13081 ctgatgcgac gcggaagcgt cttatcatgc ctacatagca ctgccacgta tgtttacacc
    13141 gcatccggca taaaaacacg cgcactttgc tacggcttcc ctatcgggag gccgtttttt
    13201 tgcctttcac tcctcgaaca attttcatat tgttgttttt gcgatcatta tctcgacatt
    13261 taaaagcaaa taatttcatt atattttgaa atcgaaagca aacgacaaga tatgaaaatg
    13321 tacgtggtat cgacaaagca gatgctgaac aacgcacagc gcggcggtta tgcggttccg
    13381 gcattcaata ttcacaatct cgaaacgatg caagtggtgg tggaaaccgc tgccaacctg
    13441 catgcgccgg tcatcatcgc cggaacgcct ggcacattta ctcatgctgg tacagaaaat
    13501 ctgttggcgc tggtcagcgc gatggcgaag caatatcacc atccactggc aattcatctc
    13561 gaccatcaca cgaaatttga cgatatcgct cagaaggttc gttctggcgt gcgctcagtc
    13621 atgattgacg cctcgcattt gccttttgcg caaaatattt cacgggtcaa agaggtggtg
    13681 gatttttgcc atcgctttga tgtcagcgtc gaggcggagc tggggcaact tggcggccag
    13741 gaagatgatg tgcaagtcaa tgaagtcgat gcgttgtaca ccaaccccgc acaggcgcgt
    13801 gaatttgccg aggcaaccgg gattgattcc ctggcggtcg ccatcggcac ggctcacggt
    13861 atgtatgcca gcgcaccggt gcttgatttt tctcgactgg agaacattcg ccagtgggtg
    13921 aacttaccgc tggtgctgca tggcgcgtca ggattatcga ctaaggatat tcagcaaacc
    13981 atcaaactgg ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc cttcttgcag
    14041 tcgttaaaaa attacctgac cgagcacccc gaagcgaccg atccccggga ttatttgcag
    14101 tcggctaaat ccgcaatgcg cgatgtggtg agcaaagtga ttgccgattg tggctgcgag
    14161 ggcagggcat aacgcacctg ccatttaaca aggaaaaaac atgaaaacgt taattgcccg
    14221 gcataaagct ggtgaacata tcggcatatg ttcagtctgt tctgcccatc cgttggttat
    14281 cgaagcggcg ctggcatttg atcgcaacag cacgcgcaaa gtgctgattg aagcaacgtc
    14341 aaaccaggtc aatcaatttg gcggttatac cggaatgaca ccggcagact ttcgcgaatt
    14401 tgtttttacg attgccgata aagttgggtt tgcacgcgaa cgcattattc tcggcggcga
    14461 tcatctggga ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt
    14521 cgagctggta aaagcatatg ttcgtgctgg cttcagtaaa attcatcttg atgcgtcaat
    14581 gtcctgcgcg ggggatacca taccgttagc accagaaacg gttgcggaac gagctgctgt
    14641 gctttgcttt gctgcggaaa gtgtggcgac agattgccag cgtgagcaac tgagctatgt
    14701 cattggcact gaagttccgg ttccgggcgg tgaggccagc gccattcagt cagtacacat
    14761 cacccatgtt gaagatgccg ccaatacttt acgtacgcat caaaaggcct ttattgcccg
    14821 tgggctgaca gaggcgttaa cacgtgtgat tgccatcgtg gtgcagccgg gtgtggaatt
    14881 tgatcacagc aatattatcc attatcagcc gcaggaagcg caggcgctgg cgcaatggat
    14941 agaaaacacc cgaatggttt atgaagcaca ttctaccgat taccagaccc ggacggctta
    15001 ttgggaatta gtccgcgatc actttgcaat attgaaagtc ggtcacgcat taacctttgc
    15061 tttacgcgag gcgatatttg cactggcaca aattgagcag gaacttatcg cccccgaaaa
    15121 tcgcagcggt tgcctggcgg taattgaaga agtgatgctc gacgaaccgc aatactggaa
    15181 aaaatattat cgcacgggtt ttaacgattc attactggat attcgttaca gcctgtcgga
    15241 tcgtattcgt tattactggc cgcatagtcg gattaaaaat agcgttgaaa cgatgatggt
    15301 gaatctgcaa ggcgtggaca tcccactggg catgattagt cagtatcttc ccaaacaatt
    15361 tgaacgcatt cagtccgggg aattatcagc aatgccgcat cagcttatta tgaataaaat
    15421 ttatgatgtt ttgcgcgcct atcgctacgg ctgtgcggaa taaggacggt atatgactaa
    15481 cctgtttgtt cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa cctatatcgg
    15541 taatgagatg ctcgcgaaag gtgtggttca tgatacctgg ccacaggcat taattgccag
    15601 agaagcagaa ttccctaccg ggataatgct tgagcagcac gccattgcaa taccgcattg
    15661 tgaggcgatt catgctaagt cgtcagccat ttatctgtta aggccaacaa ataaagttca
    15721 ttttcagcaa gcggatgatg ataacgacgt ggcggtatcg ttggttattg cgttgattgt
    15781 ggaaaatccg cagcagcaat tgaaactttt acgctgttta tttggcaagt tacaacagcc
    15841 cgatatcgtc gagacactaa ttactcttcc tgaaactcag ttaaaggaat acttcacaaa
    15901 gtatgtttta gattcagacg aataaatccc tctgtaacaa taaaaaggac tatttatgaa
    15961 acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga
    16021 aattaaagag ttgtgtcaga gtcataatat tcctgttgaa ttaatccagt gtcgggttaa
    16081 tgaaatagaa acctatatgg atggtgtgca tttgatatgt accactgcca gggtggatcg
    16141 tagttttggc gatattccgt tagttcacgg catgcctttt gtttctggtg tcggtatcga
    16201 agcattacaa aataaaattc tgactatctt acagggatga cctatgtttt cagaagtcat
    16261 gcgttatatt ctcgacctcg gccctacagt gatgctgccg gttgtcatca ttattttttc
    16321 taaaatatta ggcatgaagg caggcgattg ctttaaagcg ggtctgcata tcgggattgg
    16381 ctttgttggc attggccttg tgattggctt aatgctggat tccattggtc cggcggcgaa
    16441 agcgatggcg gaaaatttcg acctgaatct gcatgtggtc gatgtcggct ggccgggctc
    16501 ttcaccaatg acctgggcgt cgcaaattgc gctggtggcg attccgattg cgatattggt
    16561 taacgtggcg atgttactga cccgtatgac gcgggtggta aatgttgata tctggaatat
    16621 ctggcatatg accttcaccg gcgcgttgct gcacctggca accggttcat ggatgatagg
    16681 gatggcgggt gtggtaattc acgcggcgtt tgtttataag ctcggcgact ggtttgcccg
    16741 cgatacccga aatttctttg agctggaagg tattgctatt ccgcacggta cgtcggcgta
    16801 tatggggccg attgcggtgc tggtcgatgc tatcatcgag aaaatcccag gcgttaaccg
    16861 aattaaattt agcgccgacg atattcagcg caaatttggc ccttttggcg aacctgtcac
    16921 cgtgggtttt gtgatggggc tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt
    16981 attgcagctg gcggtaaaaa cggcggcagt tatgctgctg atgccacgga tgattaaacc
    17041 catcatggat ggtttaacgc ccatcgctaa gcaggcccgt agtcgtttac aggcgaagtt
    17101 cggcggtcag gagttcctga ttggcctgga tcccgcatta ctgctggggc atacggcggt
    17161 ggtatcggca agcctgattt ttattccgct caccatttta attgctgttt gtgttccggg
    17221 taatcaggta ctgccgtttg gcgatcttgc cactatcggc ttcttcgtgg cgatggcggt
    17281 cgcggtgcat cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca ttatgagcat
    17341 caccctgtgg atcgcgacgc aaactattgg tttgcacacc caaccggcgg ctaatgctgg
    17401 ggcgttaaaa gccgggggta tggtggcttc aatggatcag ggcggttctc ccattacctg
    17461 gttactgatt caggttttct cc
//
LOCUS       DAJVPT010000085        17352 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVPT010000085 DAJVPT010000000
VERSION     DAJVPT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            116..325
                     /locus_tag="OQM47_002826"
     CDS             116..325
                     /locus_tag="OQM47_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1480913.1"
                     /translation="MAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGSDFTQ
                     ALLGHRSSSMTDKYRDGRGREWKDI"
     gene            561..1379
                     /gene="ybhA"
                     /locus_tag="OQM47_002827"
     CDS             561..1379
                     /gene="ybhA"
                     /locus_tag="OQM47_002827"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1480914.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1380..2438)
                     /gene="modC"
                     /locus_tag="OQM47_002828"
     CDS             complement(1380..2438)
                     /gene="modC"
                     /locus_tag="OQM47_002828"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1480915.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2441..3130)
                     /gene="modB"
                     /locus_tag="OQM47_002829"
     CDS             complement(2441..3130)
                     /gene="modB"
                     /locus_tag="OQM47_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1480916.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3130..3903)
                     /gene="modA"
                     /locus_tag="OQM47_002830"
     CDS             complement(3130..3903)
                     /gene="modA"
                     /locus_tag="OQM47_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1480917.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4070..4219)
                     /gene="acrZ"
                     /locus_tag="OQM47_002831"
     CDS             complement(4070..4219)
                     /gene="acrZ"
                     /locus_tag="OQM47_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1480918.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4348..5136
                     /gene="modE"
                     /locus_tag="OQM47_002832"
     CDS             4348..5136
                     /gene="modE"
                     /locus_tag="OQM47_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1480919.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5204..6676
                     /gene="modF"
                     /locus_tag="OQM47_002833"
     CDS             5204..6676
                     /gene="modF"
                     /locus_tag="OQM47_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1480920.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6937..7953
                     /gene="galE"
                     /locus_tag="OQM47_002834"
     CDS             6937..7953
                     /gene="galE"
                     /locus_tag="OQM47_002834"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1480921.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7963..9009
                     /gene="galT"
                     /locus_tag="OQM47_002835"
     CDS             7963..9009
                     /gene="galT"
                     /locus_tag="OQM47_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1480922.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9013..10161
                     /gene="galK"
                     /locus_tag="OQM47_002836"
     CDS             9013..10161
                     /gene="galK"
                     /locus_tag="OQM47_002836"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1480923.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10155..11195
                     /gene="galM"
                     /locus_tag="OQM47_002837"
     CDS             10155..11195
                     /gene="galM"
                     /locus_tag="OQM47_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1480924.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11398..12150
                     /gene="gpmA"
                     /locus_tag="OQM47_002838"
     CDS             11398..12150
                     /gene="gpmA"
                     /locus_tag="OQM47_002838"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1480925.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12317..13369)
                     /gene="aroG"
                     /locus_tag="OQM47_002839"
     CDS             complement(12317..13369)
                     /gene="aroG"
                     /locus_tag="OQM47_002839"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1480926.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLSAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13685..14065
                     /locus_tag="OQM47_002840"
     CDS             13685..14065
                     /locus_tag="OQM47_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1480927.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14179..15120
                     /gene="zitB"
                     /locus_tag="OQM47_002841"
     CDS             14179..15120
                     /gene="zitB"
                     /locus_tag="OQM47_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1480928.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15117..15836)
                     /gene="pnuC"
                     /locus_tag="OQM47_002842"
     CDS             complement(15117..15836)
                     /gene="pnuC"
                     /locus_tag="OQM47_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1480929.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15874..16917)
                     /gene="nadA"
                     /locus_tag="OQM47_002843"
     CDS             complement(15874..16917)
                     /gene="nadA"
                     /locus_tag="OQM47_002843"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1480930.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17166..17241)
                     /locus_tag="OQM47_002844"
     tRNA            complement(17166..17241)
                     /locus_tag="OQM47_002844"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17206..17208),aa:Lys,seq:ttt)
     gene            complement(17245..17320)
                     /locus_tag="OQM47_002845"
     tRNA            complement(17245..17320)
                     /locus_tag="OQM47_002845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17285..17287),aa:Val,seq:tac)
BASE COUNT         4404 a         4550 c         4305 g         4093 t
ORIGIN      
        1 ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac tggataactt
       61 gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca ggacaattgc
      121 agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg acccaccgcc
      181 atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag gcagtgattt
      241 cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc gtgacggacg
      301 agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat gccacttgta
      361 attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat gctcctttgc
      421 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
      481 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
      541 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
      601 tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc cgcgctcgcg
      661 aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
      721 atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt
      781 atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctgcaactca
      841 ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat gcaatggtct
      901 atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg
      961 aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa caagttaacg
     1021 ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat tttggtaagc
     1081 atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg
     1141 cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggttggt
     1201 cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg ctggaagccg
     1261 ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg
     1321 tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat
     1381 caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcgcc
     1441 agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc gacttccagc
     1501 tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta
     1561 cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg
     1621 atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg ctgatcgccc
     1681 agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg
     1741 ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca cacttcctcc
     1801 agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg
     1861 aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc ccgtgtcagc
     1921 cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca
     1981 tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac gcgctgtttt
     2041 tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc
     2101 accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag attgccacgc
     2161 actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg acgcttttcc
     2221 ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag gacaatccgc
     2281 cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg
     2341 gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat ggtcaggcaa
     2401 tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc gcccgttcac
     2461 ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa
     2521 taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg gtatacatgg
     2581 cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg gttgcaccaa
     2641 actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca
     2701 gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct
     2761 gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa
     2821 acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa
     2881 accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact aataataagt
     2941 aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt
     3001 tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa
     3061 acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg
     3121 tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg cctgcggtcc
     3181 cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca caacagcaac
     3241 cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt
     3301 gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac gttcgaccag
     3361 cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg tatcccatgc
     3421 gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt ccggatcgcc
     3481 aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt cgatggtgaa
     3541 atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg
     3601 acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc
     3661 agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag tagatgacga
     3721 agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag caatgtcctg
     3781 cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc
     3841 attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc atttacgagc
     3901 cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta actcggaatt
     3961 ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg gcaggaaaag
     4021 caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat caatgatttt
     4081 gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt
     4141 aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg aataccagac
     4201 tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta
     4261 agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca
     4321 caataagact tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc
     4381 caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat tgcgctttcc
     4441 ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc
     4501 attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa
     4561 ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta
     4621 ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg
     4681 aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg
     4741 ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg
     4801 gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc acgtctgggg
     4861 ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg cattactcag
     4921 gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag tcatattgag
     4981 cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac actgtgcgcc
     5041 acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac ggcctacttt
     5101 aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa
     5161 cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat
     5221 tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt
     5281 aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt cggcgctggc
     5341 ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca
     5401 catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat ggcaacggaa
     5461 taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg ctgagattat
     5521 tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt tcggtattac
     5581 cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct
     5641 gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct
     5701 ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat
     5761 tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc agtttgctgg
     5821 cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc tccagcaagc
     5881 actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga
     5941 tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc tgaacaatgg
     6001 cgtggtttct tataatgatc gccccattct taataacctt agctggcagg tgaatccagg
     6061 cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat taagcctgat
     6121 tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc gacgtcgcgg
     6181 cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca gtagtttgca
     6241 tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct attttgattc
     6301 gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga
     6361 tattctcggc attgataaac acacggctga cgctccgttc catagtcttt cctggggaca
     6421 gcagcgtttg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc ttattctcga
     6481 tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt ttgttgatgt
     6541 gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc
     6601 tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc gctatgtgct
     6661 gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa gcgcactctc
     6721 cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat aaaaagcgca
     6781 atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac ggctaaattc
     6841 ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt tgttatgcta
     6901 tggttatttc ataccataag cctaatggag cgaattatga gagttctggt taccggtggt
     6961 agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca tgatgtcatc
     7021 attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc
     7081 ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat gaccgagatc
     7141 ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc cgtcgccgaa
     7201 tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct gcgcctgatt
     7261 agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc caccgtttat
     7321 ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc gcaaagccct
     7381 tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa agcccagccg
     7441 gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat
     7501 atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc ccaagttgct
     7561 gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga agatggtact
     7621 ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa
     7681 aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt aggcagcagc
     7741 gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa ctatcatttt
     7801 gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa agccgaccgt
     7861 gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac ctggcactgg
     7921 cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca atttaatccc
     7981 gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct ggtttcaccg
     8041 caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca ggtgttacct
     8101 gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac
     8161 cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat gtctgacacg
     8221 ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc
     8281 cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag cgttgcggca
     8341 ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa aacgtacccg
     8401 tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc gcatccgcac
     8461 ggacagattt gggcaaatag cttcctgcct aacgaagctg agtgcgaaga ccgcctgcaa
     8521 aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca gcgcgagctg
     8581 gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt gccttactgg
     8641 gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg gatcaccgat
     8701 ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac cagtcgttat
     8761 gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc accatttaat
     8821 ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct gctgcgctcc
     8881 gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg
     8941 accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc
     9001 ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc
     9061 taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacc
     9121 gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagctgt
     9181 gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa tcagctcgac
     9241 gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc gaactacgtt
     9301 cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt ggacatggtg
     9361 atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg
     9421 gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca aatcgcgctt
     9481 aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat ggatcagcta
     9541 atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc actggggact
     9601 aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt
     9661 accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc
     9721 ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt tgcacatgag
     9781 ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc ccgcaccgtt
     9841 gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt gatggccgag
     9901 tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga cactctggta
     9961 gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt
    10021 ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc
    10081 gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg taaaccatca
    10141 caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt cagccgtacc
    10201 gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga
    10261 ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct
    10321 gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct attggtcgtt
    10381 atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg acgctttcgc
    10441 caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc
    10501 agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat gatggcgatc
    10561 agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac gataaccgta
    10621 tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg
    10681 tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg
    10741 cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg aaatctgtcg
    10801 ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg
    10861 acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc aaaggcgatg
    10921 gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg aaggtctaca
    10981 ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca ccgtcgcggg
    11041 gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt ctgccggaca
    11101 gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt
    11161 ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc atgagggctt
    11221 tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca
    11281 acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc
    11341 gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag taagcatatg
    11401 gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt
    11461 ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca
    11521 ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc tgtgctgaaa
    11581 cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt
    11641 gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg
    11701 gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg
    11761 actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg
    11821 aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg
    11881 atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc
    11941 gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat gagcgaagaa
    12001 gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt cgacgagaat
    12061 ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca
    12121 gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat gagaaagccg
    12181 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
    12241 cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc
    12301 atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt tgacgtaaca
    12361 gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg taggccagcg
    12421 gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
    12481 taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct
    12541 ttttgaattg tttggacgaa ttagcatggc tgaaatcgat catcacctga gctgacagac
    12601 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
    12661 taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca atcgccgaat
    12721 gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga
    12781 tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa gaaagaccag
    12841 acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg ccccagctca
    12901 tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc gctggcagac
    12961 cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga
    13021 agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg gtacgcggct
    13081 tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca
    13141 gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac gggccaatca
    13201 ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg
    13261 caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg acaggaggaa
    13321 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag
    13381 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
    13441 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
    13501 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
    13561 tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc aatgccttat
    13621 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
    13681 tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag
    13741 cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc
    13801 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
    13861 caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg gttcttcaat
    13921 gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg
    13981 atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac
    14041 caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc
    14101 tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata
    14161 attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa
    14221 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta
    14281 gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg
    14341 ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct
    14401 cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat
    14461 gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc
    14521 acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg gttgctggca
    14581 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga
    14641 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
    14701 ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg
    14761 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa
    14821 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg
    14881 gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg
    14941 ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac
    15001 tacctgatgg atcactatca gattgagcac accaccattc agatggaata tcaaccttgt
    15061 cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
    15121 tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg
    15181 agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
    15241 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
    15301 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
    15361 aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg
    15421 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
    15481 gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc
    15541 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg
    15601 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
    15661 ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca
    15721 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
    15781 ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt
    15841 cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc
    15901 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga catgaacctc
    15961 gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat tcatggccat
    16021 ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg gtgcttccag
    16081 taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat cggttgccac
    16141 aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg tggaaccgac
    16201 cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc
    16261 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aatgcatcat gcacaatgca
    16321 ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg
    16381 tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac
    16441 ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
    16501 gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt cttcaacagg
    16561 gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg ttttttccgg
    16621 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc
    16681 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
    16741 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
    16801 ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg
    16861 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt
    16921 accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt cgctgttatc
    16981 ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
    17041 ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg atgtcatcag
    17101 gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt
    17161 tgcattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    17221 taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg ccgaccccct
    17281 ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc tggatactac
    17341 aaactacttc ca
//
LOCUS       DAJVPT010000086        16761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVPT010000086 DAJVPT010000000
VERSION     DAJVPT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..341)
                     /locus_tag="OQM47_002846"
     CDS             complement(<1..341)
                     /locus_tag="OQM47_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1480931.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFV"
     gene            complement(349..699)
                     /gene="chbA"
                     /locus_tag="OQM47_002847"
     CDS             complement(349..699)
                     /gene="chbA"
                     /locus_tag="OQM47_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1480932.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(758..2106)
                     /gene="chbC"
                     /locus_tag="OQM47_002848"
                     /pseudo
     CDS             complement(758..2106)
                     /gene="chbC"
                     /locus_tag="OQM47_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2191..2511)
                     /gene="chbB"
                     /locus_tag="OQM47_002849"
     CDS             complement(2191..2511)
                     /gene="chbB"
                     /locus_tag="OQM47_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1480933.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2810..3148)
                     /gene="osmE"
                     /locus_tag="OQM47_002850"
     CDS             complement(2810..3148)
                     /gene="osmE"
                     /locus_tag="OQM47_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1480934.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3350..4177
                     /gene="nadE"
                     /locus_tag="OQM47_002851"
     CDS             3350..4177
                     /gene="nadE"
                     /locus_tag="OQM47_002851"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1480935.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4407..5294
                     /gene="cho"
                     /locus_tag="OQM47_002852"
     CDS             4407..5294
                     /gene="cho"
                     /locus_tag="OQM47_002852"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1480936.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5254..5829)
                     /gene="ves"
                     /locus_tag="OQM47_002853"
     CDS             complement(5254..5829)
                     /gene="ves"
                     /locus_tag="OQM47_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1480937.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6032..6517)
                     /gene="spy"
                     /locus_tag="OQM47_002854"
     CDS             complement(6032..6517)
                     /gene="spy"
                     /locus_tag="OQM47_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1480938.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6847..7815)
                     /gene="astE"
                     /locus_tag="OQM47_002855"
     CDS             complement(6847..7815)
                     /gene="astE"
                     /locus_tag="OQM47_002855"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1480939.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7808..9141)
                     /gene="astB"
                     /locus_tag="OQM47_002856"
                     /pseudo
     CDS             complement(7808..9141)
                     /gene="astB"
                     /locus_tag="OQM47_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9138..10616)
                     /gene="astD"
                     /locus_tag="OQM47_002857"
     CDS             complement(9138..10616)
                     /gene="astD"
                     /locus_tag="OQM47_002857"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1480940.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10613..11647)
                     /gene="astA"
                     /locus_tag="OQM47_002858"
     CDS             complement(10613..11647)
                     /gene="astA"
                     /locus_tag="OQM47_002858"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1480941.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11644..12864)
                     /gene="astC"
                     /locus_tag="OQM47_002859"
                     /pseudo
     CDS             complement(11644..12864)
                     /gene="astC"
                     /locus_tag="OQM47_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13310..14116
                     /gene="xthA"
                     /locus_tag="OQM47_002860"
     CDS             13310..14116
                     /gene="xthA"
                     /locus_tag="OQM47_002860"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1480942.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14283..14993
                     /locus_tag="OQM47_002861"
     CDS             14283..14993
                     /locus_tag="OQM47_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1480943.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            14998..15675
                     /gene="ydjY"
                     /locus_tag="OQM47_002862"
     CDS             14998..15675
                     /gene="ydjY"
                     /locus_tag="OQM47_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1480944.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15690..16397
                     /locus_tag="OQM47_002863"
     CDS             15690..16397
                     /locus_tag="OQM47_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1480945.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16397..>16761
                     /locus_tag="OQM47_002864"
     CDS             16397..>16761
                     /locus_tag="OQM47_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1480946.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEA"
BASE COUNT         4145 a         4463 c         4093 g         4060 t
ORIGIN      
        1 acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa aaaagcgttt actgatccca
       61 acgttcaata tgcgcgtggc accaaactca taaaaacttt gatggtgcga acctaacgga
      121 ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc ctggaaataa
      181 cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagtcc gctgatactc
      241 tcagttttgt tatagataaa cacatggaag tttttgccat taaacaactg ctgttctcgg
      301 gcagtggcaa tttccggcgc gttaatcact ggctgcatca tcgactcctt atgccttcag
      361 tttttcatga agctcaatta attcagtaat cagttcacgc gcaagcatgg acgtcattaa
      421 atgatcctga gcgtggacca gcaccagact cactttcatc ttaccttcgc ccgcatcgcc
      481 ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat gccatccgtg actgatccat
      541 catggctttc gctgcggcaa aatcgccctg cttcgcctgt ttcaatgcgg catacgccag
      601 gctacgcgct tgtccagagt tgatgataag ccccatcacc acttcttcca gctcttcagc
      661 ttctgtttgc gtatcaggaa tattatcgag atccatcata catcgttcct ctctcttctt
      721 accggcaaga ttacccgtac cggcatcgat taaaatttca ggggttagcg atatcttctt
      781 cgctctcttc tttatcaatc gcattttgtg ctttgttagc caccacaaca aagggcagat
      841 aaattaacgt tgcgatgcca aggttgaaga gtgcgaccag caatgcggcg acgctaccgt
      901 tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata ttggtcaccg
      961 gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc ggttgtacca
     1021 gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac agaatcggtt
     1081 cattaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga taatcagcac
     1141 ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg cccccaagga
     1201 aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg gctgccagcg
     1261 ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca
     1321 taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag aaccagagca
     1381 gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg
     1441 agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac gcccaggcaa
     1501 taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaaa gaacgcgata
     1561 ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa
     1621 acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc ccacctaacc
     1681 agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg
     1741 caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc
     1801 caataaagaa cggtgccatt aaagacatta ttcctaatgt tccgttatat acgttgccgc
     1861 caataccttt cagaccatta agtgtttcaa tggttgaggc gtcgaggcga atacctaagg
     1921 aataaaaaac gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc
     1981 ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg
     2041 ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt
     2101 actcatagaa aaataccctt aaaaaccgca atttaaatat tacggtattg atttatgaaa
     2161 taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc
     2221 aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac
     2281 ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg
     2341 ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc
     2401 ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa
     2461 agagatagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc
     2521 ctcatcgacg attatctgtc agccagacac tccgcaagcc ttgacctgct tccatgctct
     2581 gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac
     2641 ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt ggttatgacc
     2701 ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc caaaaaaaac
     2761 cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat tacttcgcag
     2821 cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg
     2881 tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg
     2941 tctggcaagt accgcgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt
     3001 gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact
     3061 ggtctttggt acgatcataa gctgtacaac ccgccagcat ggttaatacc gccgctgcac
     3121 tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat
     3181 aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga
     3241 ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc
     3301 aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca
     3361 acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg
     3421 tcgtagtgtc gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt
     3481 gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc agatggcgat
     3541 taatgagctg cgccaggaaa ctcgcaacga atcactgcaa tttattgccg tacgcctgcc
     3601 ctatggtgtt caggctgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga
     3661 tcgcgtatta accgttaata tcaagggcgc ggtattggct agcgagcagg cattgcggga
     3721 agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa
     3781 agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc
     3841 agaagccatt accggattct tcactaaata tggtgacggc ggtacggata ttaatccgct
     3901 gtatcgtctc aacaaacgcc agggtaaaca gttactgacg gcattaggtt gcccggaaca
     3961 cctttataag aaagccccaa ctgccgatct tgaagatgat cgcccttctc tgccagatga
     4021 agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacctacc
     4081 tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc
     4141 gccaattaac gttttcgatg atttctggaa aaagtaataa tttgcaccta ttggattgtg
     4201 cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattct acctacaaaa
     4261 ctatgcaaat tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac
     4321 ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc ctcccgcctg ttacactgga
     4381 tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat
     4441 ttgaagctgc ggcaatttat gaatatcccg aacatttaca ttcattcctt aatgacttac
     4501 ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg
     4561 gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg
     4621 ccatgctacg gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa atcggcgctc
     4681 tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa cggttgcgcc
     4741 gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca
     4801 aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag
     4861 ctttgcaagc attgcagagc atcgctgatg aacaaaaact ttgttatggc ctgttgggac
     4921 tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag
     4981 catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag tctctggagc
     5041 gtttgcgagt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa cagcacccgg
     5101 aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctgg
     5161 aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt tataaaattc
     5221 tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg gcgaatgacc
     5281 agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt
     5341 gtgcctgcca tcgaaccagc acacgccctg gtcggtagtg agcagtttgt cgcccagttg
     5401 ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag taaacgtacg
     5461 ttcggcaatt ctgactttcg ccttacagac atccaggcgc gtcataatat tgaaatccat
     5521 cgacatctgc cctgccgtca gtttcgcttt taccacctgg tccgccgtaa aggcaaaagg
     5581 ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca tctcgccgcc
     5641 ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc cattcgccgc
     5701 gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat
     5761 ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc gacattttac gcatatcaaa
     5821 gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat
     5881 ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt acaactgttt
     5941 gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag
     6001 accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc attttacctt
     6061 ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt
     6121 ccggcgtcag gatgttgtaa attttgttct gggtttccat gtgcgccagc atgttagctt
     6181 tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc
     6241 tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac
     6301 gctggccttt catgatttcg tggatctgct gtttctgcgc gtcggtcagg ttcaggtctt
     6361 tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt
     6421 cagccggtgc tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca
     6481 gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt
     6541 atttattacg gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta
     6601 gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa
     6661 ttatggagaa aaaaagaaaa tttgatggag agtgatgaga gaatattaca acacgatgat
     6721 tttgcagaga ttatgaagaa ctataccgga tgactggtga taaataaagc aaataaccag
     6781 gattaatctg tattaattta taagaaagca acttaatacc cgcagaatga tttctgcggg
     6841 taagtattag cttatttttt cgagcattaa tcccgcgcgt aatcccaacg ctaccaacgg
     6901 attagggaat aacacatact ctacatcatg ggttacggta aaacgttcct ctccgtcctg
     6961 cgccagcaat gttcctttct caaacggcat aaaattcagc gtgtcacttg ccatatgcat
     7021 ttcgaaggac ggcgagtggc gagtaatttg cgaaaccacc cgataacgga gcggcggtgt
     7081 tctcacgata ccgacactct caccagatag cagcgcagca attgcgctgg cagttactgc
     7141 aaactggcga agatcgtttt gcccaaaggg caacgctttg ccaagttcca acgtacaggc
     7201 cagcgcgcca aaatgtctga cgctgaaatg ggtaaacgta ccaccaggct cctgatggaa
     7261 caccagcgcc tccagccccg ccgcacccag ccacgtcaga aatttctcgt cccagggaat
     7321 gtcgcgttgc ggtaatacac cgaactgcgg atgcaaggag ccacgaattg cggtatgtag
     7381 atcaaggtgc caacgcacag attctttgcc ctggtcataa aaatcttcca ggcactgttc
     7441 cagttcgcgc gcccgacagg tttctccgct ttcagcaaat agctgccaac gaccgccaaa
     7501 cattcggttc atatcgctat ggcaataacg tttcccttgc ttcagcgcag gaggattccc
     7561 gaggatcacc agcaaccgcc aacgtaacgg gatctcgccg tgagatatcg cgccaagcaa
     7621 cgcgtccagc atttccacag gtgccgtctc attaccgtgg atccccgcag aaatcaccag
     7681 cgcgccttgc ggtggcgtta atggcgtcag ttccagcaca ccatcgccca gccagcgcca
     7741 gcgaacgccg ttgatttctc gctcggtgat aaccggtttt ttacccgtta aggtcagagc
     7801 aagaaaatta tccattgccc cctccctcgc gctggaacgg ataaaccgaa ccgagattca
     7861 gtaattggct caatacatcc agtgcttccc gcccttcgcg cagcaattgc gggtcggcca
     7921 gatcggcagc agtaagacga tcgcggtagt aacgatccac ccagtcattg agcgcattaa
     7981 acaaggtatc gttcatcatc accgccggat tcaccgcccg gcgttcttct tctgtcaata
     8041 ccacccgcaa ccgcaggcac gccgggccgc cgccattcgc catgctttca cgtaagtcaa
     8101 agacttttag ttcgctaatc gggttgtcag cggcaaggag ttcattgaga taaccccata
     8161 ctccggcgtg ttcccgacac tcctgaggca gcaccaacat catggaacca tcatcgcggc
     8221 tcaacagttg gctgttaaac agataggtag acaccgcatc cgacacggaa acctgagttg
     8281 ccggaacttc tatcgccata aaaccgttca cccgcgtgcc agtaactgtg actggcgagc
     8341 gaacgcctgt tggtggcaaa acagcacctg gcggttactc acggcaatca cgtcattatg
     8401 aaaaacgctc tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct gttggggatt
     8461 cacctgatta agccttgcca ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg
     8521 ggaaggccgg gtatcattgc cttcttctcg cccgtagaca aaaagtagca tacccggttc
     8581 accgtaatga ccgccgagac gattgtggtt tgccgccccc tcatcaccga gcaacgctac
     8641 ctgcggcaac gccgaatgga cgctaaattt ctcttcgtcc ttaaaaatcg cttttaacag
     8701 cgattcagtg acgggcgctt ccagcgaacg gtgaaattta ttgttcaggt tggcaacggt
     8761 gagatgcact ttgccatcca gcgtatcggc agatggcgcg atcgttgccg cattggctac
     8821 ccacattggc gaagcggaac tgacgctgga aagccagtgc ggtgcctggc gtgcaacttt
     8881 ttccagtacc tgctcatcgc taccactgaa tcccaactga cgcagcaccg gaataaacgg
     8941 acgctcgtgc ggcggaatca cggcctgggg gaatcccgca tcggcaaggg ctttcatttt
     9001 cagtaagccc tgttttgccg ccagtcgcgg gttagacacc tgaaaacggt gacgggtaga
     9061 ggcttcatta ccaaacgaca ggcccgcgta atgatgcgtc agccctacca gcccgtcgaa
     9121 attaacttcc caggcgttca tcgcaccacc tcatcggaaa aatccagccc ggggttaagc
     9181 gtggcgggca atgttaacga gtccgactcc aggctcgcca taggccatgc gcagtaatct
     9241 gcggcatacc aggcgctggg gcgatggtta ccggaagcac caatgccgcc gaatggggcg
     9301 gtactggcag caccagtaag cggtttgttc cagttaacaa tccccgcccg cgcctccagc
     9361 aacagttgat cgaatttttc ccgctcgggg gaaaccagac cgcaaaagag tccgaagcga
     9421 gtgttgttcg ccatccgaat cgcctcatca aaagtatcat aacgccagac gcgcaaaaac
     9481 ggcccgaaca cctcttcatc cggcacaccg ccaacgcccg tcatttcaat gatccctggt
     9541 gtcagcaacg atgtccctgc ttgtaataag cgcggcgcaa gcagggttcg tccgcccatc
     9601 gcttccagtt gctgccaggc agtaaccacc tgctgtgcgg cctgttcaga aatcagcccg
     9661 ccaataaacg gctgcggttc gtcatcccag ttgcccggcg ttaatcgctg gctgacggca
     9721 accagactag caagaaacgc atcgccctgc gctccgcttt tgagcaataa acggcgggcg
     9781 caggtgcagc gttgcccggc tgtgacaaac gccgactgaa tggtcagatg gacagccgcg
     9841 tcgatatccg ccacctcatc gataattagc ggattattac cgcccatctc aagggcgaga
     9901 attttctccg gctgaccgga gagctggcga tgcagctggt agccggtatt ggcgctaccg
     9961 gtaaacagca aaccgtcgag atcctccagc gcactcagcg cctgacccgt ttcacgcccg
    10021 ccctgcacca ggttcagcac gcccggcggc aagccagcct gctgccataa acgcattacc
    10081 gcttcgccac tccacggtgt cagttcgctg ggtttaaaga taatggtgtt acctgccagc
    10141 aatgccggaa cgatatgtcc gttcggcaaa tgaccaggga aattatacgg cccaaacacc
    10201 gccagcacgc cgtgcgggcg atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc
    10261 tgctcgccgg tacgaacgtg atacgcctta attgatatcg cgattttatt gatcatcgcc
    10321 gtcacttcgg ttgccgcttc ccagcgcggc ttacccgttt ctctggcaat aatcgcggtt
    10381 aattcggctt tattgctttc cagcaatccg gcaaagcgtt cgacaacgac ctgacgttca
    10441 gccaatgaga gccgcgccca gcgcggaaac gccgcacggg ctgcccgaca agcctgttcg
    10501 acctgagcgg catcggcatc attgccttgc cataacacct cgcccgatac cggattacgc
    10561 ttcacacgcg atgcgccctg gcccgttatc cagtcaccgt taatccataa agtcatgctg
    10621 ttttctcctc tgcgcacagg cgcaccagac gaacgcgatc ccctgcgtgg catttgaggg
    10681 catccagttg tgcggcggtt aaaatcaaac gctcggttgc cggatcggta cgcgccagca
    10741 ccacgcggaa atggtgataa ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg
    10801 caggctgccc ttctgccact tccaccagcc gacttttacg gatggcgcgc acgcggtcga
    10861 tgtcacactc aagcgttggc ccgccgtcga agatgtcgat atagttgcgg taacgaaaac
    10921 cttctttctc cagcaccgcg cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt
    10981 cctgggcttc ctgggataaa aagtgggtat agatcggatg tttcggcatc agttctgcaa
    11041 taaatgcctt ttgcccggtg ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga
    11101 agcgtttacc gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc accccgcgca
    11161 tttcagcaac cactttgtca ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg
    11221 atttcgatag caaatagccg ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc
    11281 acagctcgct gctgccggtg tgatcgttac taagaaacag cgtcggcaat gcgttataga
    11341 cattcagctc ttttgaggcg tgaaccaacg tgccgacgcg atagttgtac cagggatcgt
    11401 tcagcccaac cgccacctca atagcgcaaa tccccgccac ggtgcctgtc tcgctatctt
    11461 ccagcacgaa cacatagccc tgctcgcttt tgggcagttc accttgccag gttttgattg
    11521 ccctttcgat acgcgccgaa agcgtggctt cattggcggg aagcgacgtc aggccgccgc
    11581 ccgttttgct ggcaagctgc atcagcgccg agacatctga gcgctcaacg ggacggatga
    11641 ccatcatgat gaacctcggc taacaaagtg ttcgcaagca gctgcaaagc gatccagtcc
    11701 ggtcgtcacc tcttcttcgc tgacattgag cgcaggcgca aaacgcacca cgttgccacc
    11761 cgcaatcagt accatcacgc ctgctttcgc cgcttccaga gagatctgtt tcgcttgccc
    11821 ggcgtaatcg gcattcagta cacagccaat caacaaacct aagccgcgaa cttcactgaa
    11881 caaaccatag cggtgattaa tagtattaag acgctcaaca aaccagtcgt gacgctgttt
    11941 aacgccatta agcatctctg gtgtgttgat gagctccagc actttgcctg ctaccgccga
    12001 ggccagcgga ttaccgccat aggtggtgcc atgagtgcca acggtcatca cgcgggcgca
    12061 ctcttcggtt gccaacaacg caccgaccgg gaaaccgccg cccagcgctt tggcggtggt
    12121 taacagatcg ggcgtcacgc cgtagtgcat ataggcatac agctcaccag tgcgcccgac
    12181 gccggtttgt acctcatcaa aaatcagcaa ggcattgtgg cggtcacaca attcacgcag
    12241 accttgtaaa aacgcgttgc tggctggcac cacaccgcct tcgccctaga tgggctcgac
    12301 aatcaccgca caggtggcgt cgtcaatcag cgcgctggca gagttaatat cgttatatgc
    12361 agcatgacga atatccgccg gcagtggcgc aaaatcctgt gaataggctg gctgcccacc
    12421 cgcactgaca gtaaacagcg tgcgaccatg aaacgcattt ttgaacgcca cgatgccact
    12481 cttatggctg ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt
    12541 ggcttccgca ccggagttac aaaagaagac gcgatcggca aacgtggcgt cgatcaattt
    12601 tttcgccagt cgcagtaccg gctcgttggt gtaaccgttg ccggtatgcc agaacttact
    12661 cgcctgttcg ttcagcgctt cacgcagttc cggatgcgca tggcccagcg cgttcaccgc
    12721 aatgccaccc gcgaagtcga tatactcttt cccctgctga tcccacaagc gcgaaccttc
    12781 gccacgtacc ggtataaagg gtgccggagc gtaaacaggt atcatccatt catcaaagtt
    12841 ttcacgcgta attggctgag acatagcgac ctctacagta aataattcgt tatttgtatg
    12901 ttaacaataa gtaatgtttg cgctgtaaat gtagattgca gggttcgtgc cagccagtga
    12961 taaaagtgca taaacggcgg aggctaactg gaaatcaagg agttataacc aaaccatatg
    13021 catttaaagt gcatataaag tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa
    13081 acgcaatgtt atgcagaagt aaaatataat tctagaattg tgatcattga cgaaatttac
    13141 tggaaattac tgcgccattc tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat
    13201 cgttgacatc attaacaacc atcgatcaaa tcacttaaca acaggcggta agcaacgcga
    13261 aattctgcta ccatccacgc actctttatc tgaataaatg gcagcgacta tgaaatttgt
    13321 ctcttttaat atcaacggcc tgcgcgccag acctcaccag cttgaagcca tcgtcgaaaa
    13381 gcaccaaccg gatgtgattg gcctgcagga gacaaaagtt catgacgata tgtttccgct
    13441 cgaagaggtg gcgaagctcg gctacaacgt gttttatcac gggcagaaag gccattatgg
    13501 cgtggcgctg ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc ccggtgacga
    13561 cgaagaggcg cagcggcgga ttattatggc ggaaatcccc tcactgctgg gtaatgtcac
    13621 cgtgatcaac ggttacttcc cgcagggtga aagccgcgac catccgataa aattcccggc
    13681 aaaagcgcag ttttatcaga atctgcaaaa ctacctggaa accgaactca aacgtgataa
    13741 tccggtactg attatgggcg atatgaatat cagccctaca gatctggata tcggcattgg
    13801 cgaagaaaac cgtaagcgct ggctgcgtac cggtaaatgc tctttcctgc cggaagagcg
    13861 cgaatggatg gacaggctga tgagctgggg ggtggtcgat accttccgcc atgctaatcc
    13921 gcaaacagca gatcgtttct catggtttga ttaccgctca aaaggttttg acgataaccg
    13981 tggtctgcgc atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac
    14041 cggcatcgac tatgaaattc gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc
    14101 gaccttccgc cgctaattta gcagctctcc tggctcaaac tgggtcagga gaattaacct
    14161 tgagaaaaat caacaaactg tcagtaatga tttgttgcct gccgtccttt gttataccgt
    14221 cactgcgttt ttagttgtct gaccacttct ctattatcaa gtttgatata ggaaactcca
    14281 cgatgaacgc tgagcgtaac tttctgtttg cctgtcttat ttttgcgctg gtcatttacg
    14341 ctatccacgc tttcggttta ttcgatctgc tcaccgattt accccactta cagacactca
    14401 ttcgccagag cggattgttc ggttatagcc tctatattct gttattcatc aatgcccccc
    14461 tctttctgtt accaggaagc atactggtga tcgctggagg aatagttttt ggcccgctct
    14521 tagggacaca actctcatta attgccgcaa cgctggcctc ctcgtgctca ttcctgctgg
    14581 cgcgctggct gggacgtgat ttactgctga aatacgtggg ccatagccat accttccagg
    14641 ccattgaaaa aggcattgcg cgtaacggta ttgattttct tattctgacc cgcttaatcc
    14701 cgttgtttcc ttacaatatt caaaattacg cttacggatt caccaccatc gccttctggc
    14761 cttataccct tatttcggca ctcacgaccc tgcccggtat tgttatttat accgtgatgg
    14821 caagcgatct cgccaatgaa ggcattacgc tgcgctttat tttacaactc tgtctggcgg
    14881 gcctggcgct gtttattctc atccagctcg caaaactcta cgcccgacac aaacatgtgg
    14941 atctgtctgc ttcgcgccgc agcccactta ctcacccaaa aaatgaagga tagaacgatg
    15001 tcgcaacatt attcagtgtc atggaaaaaa ggactggctg cactctgctt actggctgtt
    15061 gccgggctta gcggctgcga tcaaaaagag aatgcggcgg caaaagtgga atacgacgga
    15121 ctttcgaaca gccaaccgct gcgcgtcgat gccaataacc atacggtaac catgctggtg
    15181 caaattaatg gtcgtttcct caccgacgac actcgtcacg gtattgtgtt taaagatggc
    15241 tccaacggac ataaatcgct gtttatggct tatgcgcccc cgaaagcatt ttatgaagcc
    15301 ctgaaagagg caggtggtac gccgggcgaa aacatgacga tggataataa agaaacgact
    15361 catgtcacag gcagcaaact ggatatttcg gtcaactggc aaggggcggc aaaagcgtat
    15421 tccttcgatg aagtgattgt tgacagtaat ggcaagaaac tggacatgcg ctttggcggt
    15481 aatttaacgg cagcagaaga gaagaaaacg ggttgcctgg tgtgtctgga tagctgcccg
    15541 gtcggcatcg tcagcaatgc aacatacact tatggtgcgg tggaaaaaca cggcgaagtg
    15601 aaattcaaag gcaatgcctc agttctcccg gcggataaca cgctggcaac ggttaccttt
    15661 aaaatcaccg aataaagcca agataaagga tgatgaagat acaatcgcgt aaaatctggt
    15721 actaccgtat aaccctcatc atcctgttgt tcgctatgct gctcgcatgg gcgctgcttc
    15781 ccggcgtcca tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg
    15841 gtatagaacg ctttattcag tcttacggcg cactggcggc agttgtctcg ttcttgttga
    15901 tgattttaca ggccattgct gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt
    15961 cgctgtttgg cgcgttctgg ggcggcttgc tgtcgtggac cagttcgatg gccggcgcgg
    16021 cgctgtgctt ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaaa ttaaccggca
    16081 aaaccgtgct tgacagtatg gacggctttt tcactcgcta cggcaaacac accatcctgg
    16141 tctgtcggtt attgcctttt gtccctttcg atccaatcag ctatgctgcc ggtctgactt
    16201 caatacgttt tcgctcgttt tttatcgcca ccgggcttgg tcaattaccg gcgactattg
    16261 tttattcctg ggcaggcagc atgttaacag gcggtacttt ctggtttgtc accgaactgt
    16321 ttattctgtt tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc
    16381 agaagaggaa tgactgatgg gtttaccgcc gcttagcaaa attcctttca ttttacgtcc
    16441 gcaggcgtgg ctgcatcgtc gccattacgg cgaggtgcta agcccgattc gctggtgggg
    16501 gcggatcccg tttatctttt atttggtgtc aatgtttgtt gggtggctgg agcgcaaacg
    16561 ctcaccgctc gatccggtag tgcgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct
    16621 gtgtgagttt tgcgtggata tcaccagtat gaaagtcgcc gagcgtaccg gcagcaccga
    16681 taaactgctg gcagtggctg actggcggca aagcccgctc tttagcgatg aagaacggct
    16741 ggcgctggag tacgccgaag c
//
LOCUS       DAJVPT010000087        16629 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVPT010000087 DAJVPT010000000
VERSION     DAJVPT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..1023
                     /gene="fepE"
                     /locus_tag="OQM47_002865"
                     /pseudo
     CDS             1..1023
                     /gene="fepE"
                     /locus_tag="OQM47_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1020..1835)
                     /gene="fepC"
                     /locus_tag="OQM47_002866"
     CDS             complement(1020..1835)
                     /gene="fepC"
                     /locus_tag="OQM47_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1480947.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(1832..2824)
                     /gene="fepG"
                     /locus_tag="OQM47_002867"
     CDS             complement(1832..2824)
                     /gene="fepG"
                     /locus_tag="OQM47_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1480948.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(2821..3825)
                     /gene="fepD"
                     /locus_tag="OQM47_002868"
     CDS             complement(2821..3825)
                     /gene="fepD"
                     /locus_tag="OQM47_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1480949.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            3936..5168
                     /gene="entS"
                     /locus_tag="OQM47_002869"
     CDS             3936..5168
                     /gene="entS"
                     /locus_tag="OQM47_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1480950.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5172..6128)
                     /gene="fepB"
                     /locus_tag="OQM47_002870"
     CDS             complement(5172..6128)
                     /gene="fepB"
                     /locus_tag="OQM47_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1480951.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6420..7595
                     /gene="entC"
                     /locus_tag="OQM47_002871"
                     /pseudo
     CDS             6420..7595
                     /gene="entC"
                     /locus_tag="OQM47_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7605..9215
                     /gene="entE"
                     /locus_tag="OQM47_002872"
     CDS             7605..9215
                     /gene="entE"
                     /locus_tag="OQM47_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1480952.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9229..10086
                     /gene="entB"
                     /locus_tag="OQM47_002873"
     CDS             9229..10086
                     /gene="entB"
                     /locus_tag="OQM47_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1480953.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10086..10832
                     /gene="entA"
                     /locus_tag="OQM47_002874"
     CDS             10086..10832
                     /gene="entA"
                     /locus_tag="OQM47_002874"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1480954.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            10835..11248
                     /gene="entH"
                     /locus_tag="OQM47_002875"
     CDS             10835..11248
                     /gene="entH"
                     /locus_tag="OQM47_002875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1480955.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11429..13534
                     /gene="cstA"
                     /locus_tag="OQM47_002876"
     CDS             11429..13534
                     /gene="cstA"
                     /locus_tag="OQM47_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1480956.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13716..13913
                     /locus_tag="OQM47_002877"
     CDS             13716..13913
                     /locus_tag="OQM47_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1480957.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(13923..15011)
                     /gene="hcxA"
                     /locus_tag="OQM47_002878"
     CDS             complement(13923..15011)
                     /gene="hcxA"
                     /locus_tag="OQM47_002878"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1480958.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15120..16280
                     /gene="ybdL"
                     /locus_tag="OQM47_002879"
     CDS             15120..16280
                     /gene="ybdL"
                     /locus_tag="OQM47_002879"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS1480959.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16281..>16629)
                     /locus_tag="OQM47_002880"
     CDS             complement(16281..>16629)
                     /locus_tag="OQM47_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCS1480960.1"
                     /translation="DKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRI
                     AATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQEL
                     FADRQYSRAWTVK"
BASE COUNT         3665 a         4370 c         4766 g         3828 t
ORIGIN      
        1 cgggccaaaa aaacggtcat ggcggtcgtt tttgcatttg cctgcgcagg tttactgatc
       61 tctttcatcc tgccgcaaaa atggaccagc tcagccgtta ttactcccgc cgaagccatt
      121 cagtggcagg acctggagaa aacctttacc aaactccgcg ttcttgatct cgatgttaat
      181 atcgaccgtg gcggtgcatt taacctgttt atcaagaagt ttcagtctgt tagcttgctg
      241 gaagagtacc tgcgttcatc accttatgtg atggactaat taaaagaggc gaaaatcgat
      301 gaactggatt tgcatcgcgc tattgttgca ttgagcgaaa aaatgaaagc ggttgatgac
      361 aatgctagta agaaaaaaga tgaaccgtca ctgtatacat cctggacgct aagttttacc
      421 gcgccaacca gtaaagaggc gcagaccgtt ttgagcgggt atatcgatta tatctctgcg
      481 ttggtggtga aagagtcgat agaaaacgtc cgtaataaac tggagatcaa aacccagttt
      541 gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa atcaacttga tgcaaacatt
      601 cagcgcctca attattcact cgacattgcc aacgcggcag gaattaaaaa gcccgtatac
      661 agtaatggtc aggccgttaa agatgacccc gatttctcta tttccctcgg cgcagacggt
      721 attgaacgca aactggaaat tgaaaaagcg gttactgacg ttgcggaact gaacggtgaa
      781 ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag caaatgtcaa cgatgtgaat
      841 tttacgccgt ttaaatatca gttaagcccg tcattgccag tgaaaaaaga cggtccgggt
      901 aaggcgatta ttgtgatcct ttccgcgctg atcggcggga tggtggcttg tggtagcgtg
      961 ttattgcgct atgcgatggc atccagaaaa caggatgcca tgatggcaga ccacttagtt
     1021 taactatttg cggttgaagg tgccgttcgt ccgagcggca ccacaagcgg cattccggct
     1081 accggatcgt caatgatcat gcatcgcaga ccataaatgc gctcaatcag ttcagcagtg
     1141 acaatctcct tcggcgctcc ctgagcaaca attttccctt cccgcaatgc aatcaaatgg
     1201 ctggcgtaac gacaggcctg attaagatcg tgcagcaccg ccgccagggt atagcctttc
     1261 tcgcggttca gttcgcttaa cagttccagt agatcaatct gatgactgat atccagccag
     1321 gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg ccagcaccat cgcgatccac
     1381 gctcgctggc gttgtccgcc agaaagggta tccacgcttt gatctgccag atgagttatt
     1441 cccgtggcct gcatcgcttt cgttaccgct tcttcatcct ctttgcgcca gcgggtaaac
     1501 agcggttgat gcggataacg tccacgcgcc accagctcct gcacggtgat atcgcccggc
     1561 gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa cttctttact ggcgtaatgt
     1621 tgaatgtgct cgccatccag ccagacatgc ccgtgagcag gcgtcatcag gcggcttaag
     1681 gtacgcagta acgtggattt accacagcca tttggtccga taattgccgt gaagtgacca
     1741 tcaggaattt ctacagtcag attttccgca acggtatatt tgccatatcc cagggttaac
     1801 tgttcgccgc gcaaacgggc tactgattcg gtcatttttt gcgagactcc tgaattaaca
     1861 agacgataag gtaaataccg ccgaggctga cggtaacgac gccaaccgga agttgatacg
     1921 gcataaacag ttgttgggcg cagagaacgg ccgccagcag taacagcgct ccgcacagcg
     1981 ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg tcgggcaatg tgcggcgcga
     2041 ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc cgcagcggta agcaccactg
     2101 caaccagcat cattaacaga cgcgaacgct cgacgctgac gcccagcgcg caggcggtat
     2161 catcgcccat ctccagcaag cgcattcgcc gcaccagcaa ggtggcggca atgagcatca
     2221 atataatgat gggtgcggaa ggcgaggttt ttgcccacgt caggccgttg agcgatccgg
     2281 cattccacaa acctgctgtt agcgccgttt ctaaagacgc tttcagcaac agccaggtat
     2341 taaaggccac cagcatggcg cgaacgccga taccgataat aatcaaccga aaggtgtcga
     2401 taccgttacg ccaggcgagc agccagacca acagcgaagt gataatgccg cccaccattg
     2461 ctgccagcgc gatagccgtc agatcctgac caaacagcac catcgccacc agcacgccgc
     2521 tccacgcccc ggtgttaaag cccattacgt cagggctgcc gagcgggtta cgcatcagcg
     2581 actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat caacagcgcc atcagcacgc
     2641 gtggtaaacg ccattcggtg accaccatcg tcatactgcg cggcgcatcg cccatcaacg
     2701 cggcgaatac ctgcgagatt tccagcgtga cggcaccgct gcgtaatccc cagatacctg
     2761 caaccacaca ggcggaaatc agcagcaaac aggtgatgag taatcggcga gagacgtaaa
     2821 tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc agcaccggtg caccaataaa
     2881 cgcactgacc acagaaacgc gcagttcgcc gggcacaatc acccgcccga tgatatcggc
     2941 aaacagcagc agggcagggg tagcaagtag cgtgacgggc agcgaccagc gatgatcggc
     3001 acctaccagc cagcgcgcca tgtgcggcat catcaggccg ataaaggcaa tcgggccaac
     3061 taccgccgtc gcactaccac aaagcacggt aatcgccagc agaccaatca actgtgtgcg
     3121 cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg aggctcaaac tgttcagcgc
     3181 gcgactcagt aatagcgcag ttgctccggc gatcagaacc gggatcagca ccacttttaa
     3241 ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag aaacgcagtt gatcgtagac
     3301 gtcaggatta agcagggcga tgccgctggt cagtccttcc agcaccgccg ccagcgccac
     3361 gcccgccagg gttaaacgca ccggacttaa ctgccctcca ccctgactgc cggtaaaggc
     3421 aacaatcaat gaggccacca gcgccccggc gaaggccatc gccagttgtt cctgtgcgga
     3481 tgagtaacca aacagcgccg cacccagcac aatggcaaag ctggctccgg cgttcacgcc
     3541 aagcaagccg gggtcggcaa gtgggtttcg ggtgagggtt tgcattaacg ccccggcaag
     3601 gccaagcgcg ccgcctgcca gtaaaccggc aagggtacgc ggcagccgcg cgtcgagcac
     3661 gatggtgcag tcggcgctct ggcaggtgcc ggagaaggcc tccagcacta cggaagcggg
     3721 gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg ataatcagta ataacagcaa
     3781 tccgggcacg gcaatggcgc gtgtcacggc aacagaacca gatatacgaa cttccatgat
     3841 aatgaaatta attatcgtta tcgatcttat ttggatatgt taacatgtgc agcctaagaa
     3901 taggtattta aaatatttga tggcaaggca ttgtaatgaa taaacaatcc tggctgctta
     3961 acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt attcctcgct cgttttatct
     4021 caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt gcagatccag atgatgaccc
     4081 attctacctg gcaggtgggg ctttcggtga cgctgaccgg cggcgcgatg tttgttggcc
     4141 tgatggttgg cggtgtgctg gcggatcgct atgaacgcaa aaaagtgatt ttgctggcgc
     4201 gcggcacctg tggcattggc ttcattggac tgtgccttaa tgcactgctg ccggagccgt
     4261 cattgctggc aatctattta cttggtttat gggatggttt tttcgcatca cttggtgtta
     4321 cggcgctact ggcggcaaca tctgcactgg tagggcgtga aaacttaatg caggccgggg
     4381 cgatcaccat gttgaccgtg cgcctggggt cggtgatttc gcccatgatt ggcggtttat
     4441 tgctggcgac cggcggcgta gcctggaact acgggctggc ggcggcgggc acgtttatta
     4501 ccttgttacc gttattaagc cttccggagt tgccaccgcc accgcagccg cttgagcatc
     4561 cgttgaaatc attactggca ggatttcgtt ttctgcttgc cagcccgctg ctgggtggtt
     4621 tattgacgat ggcgagcgcg gtgctggtac tgtatccggc gctggctgac aactggcaga
     4681 tgtcagcggc acagattggt tttctctacg cggcgatccc gctcggcgcg gctattggtg
     4741 cgttaaccag cgggaagctg gcacatagtg cgcgaccagg gttattgatg ctgctctcca
     4801 cgctgggatc gttcctcgcc attggtctgt ttggcctgat gccgatgtgg attttaggcg
     4861 tggtttgtct ggcgctgttc ggctggctga gcgcggttag ttcgttgctg caatatacaa
     4921 tgctgcaaac gcaaaccccg gaagcgatgt tagggcggat taacggtttg tggacggcgc
     4981 agaacgtgac gggcgatgcc ataggcgcgg cgctgctggg tggtttgggc gcgatgatga
     5041 caccggttgc ttccgcaagc gcgagcggtt ttggtttgtt gattatcggc gtgttgttat
     5101 tgctggtgct ggtggagttg cgacgttttc gccagacgcc gccgcaggtg acagcgtccg
     5161 acagttaatg cttaaaacag cgccttaagc cgttccagca cttgcatggc gctgtagtaa
     5221 tccaggcgga acgtctcggt tcccagcgca taaacctgct tgttttgtac cgcaggcagg
     5281 tgcgcgagca gtggattggc ataaatcgca tcggcatctt tctgatcacc agcgaacagg
     5341 aatagcgatt caccgtttaa ccccgcagcc agattttccc caccaagctg aatgatgtca
     5401 tggcgtttgc cctggctttg gctggcgttt aaccctgcgg gcaatttcgc cagcgtaaag
     5461 ccgagttgtt ccagcatctg cccttgtgct gattccggcg tccagagatt ggcactgtgt
     5521 gcagcggcgg tatagacaat ggcagtgacc ggctgcggcg gtaatttgat ttgctctttc
     5581 gccgctgcca gttgcttatc aaactgtgca atccgctctg ccgcttgttt ctcatgcccg
     5641 gtaatttcgc caagctgcgt taacagcgac tgccagcttt tgtcgtcgta attgatgatt
     5701 aatgtcgggg cgatggtgga aagctgatca tacagcgcca gcgccgaatc cccgccggtt
     5761 gcgctaatta aaatcagatc cggcatttgc gcggcaacgg cttcggcgct cggttcgccg
     5821 atatagagcc gttgcagttt gcgttctttc gccaccttgc tccactggcg taaaaagccc
     5881 tggtcatctg cgacgcggtt attcggcgtg gtcgcgccgc tggcgatcac cggagcatca
     5941 atcgccagca gtgatccggt cagggtgacg ctggtggaaa caatacgctg cggctggctt
     6001 tccagggtat gagtgccccg gctgtcagta atctgacgcg gccagtcggc ggcctgaact
     6061 acggctattc ctgaaagcaa aagtcctgtt aatagaaggg cgttgcggta gagcggggcg
     6121 agtctcacaa gtcagcttcc tgttattaat aaggttaagg gcgtaatgac aaattcgaca
     6181 aagcgcacaa tccgtccctt tggggagagg gggcaaaacg gcgcaggaca tcacattgcg
     6241 cttatgcgaa ttcatcaata atgcttctca ttttcattgt aaccacaacc agatgcaacc
     6301 ccgagttgca gattgcgtta cctcaagagt tgacatagtg cgcgtttgct tttaggttag
     6361 cggccgaaaa tataagtgat aatcattatt aaagccttta tcattttgtg gaggatgata
     6421 tggatacgtc actggctgag gaagtacagc agaccatggc aacacttgcg cccaatcgct
     6481 ttttctttat gtcgccgtac cgcagtttta cgacgtcagg atgtttcgcc cgtttcgatg
     6541 aaccggctgt gaacggggat tcgcccgaca gtcccttcca gcaaaaactc gccgcgctgt
     6601 ttgccgatgc caaagcgcag ggcatcaaaa atccggtgat ggtcggggcg attcccttcg
     6661 atccacgtca gccttcgtcg ctgtatattc ccgaatccta gcagtcgttc tcccgtcagg
     6721 aaaaacagac ctcagcccgc tgttttaccc gcagccagtc gctgaacgtg gtggaacgcc
     6781 tggcaattcc tgaacaaacc acgtttgaac agatgggtgc ccgcgccgcc gcacttaccg
     6841 ccacgccgca ggtcgacaaa gtggtgttgt cacgattgat tgatatcacc actgacgccg
     6901 ccattgatag tggcgtattg ctggaacggt tgattgcgca aaacccggtt agttacaact
     6961 tccatgtccc gctggctgat ggtggcgtcc tgctgggggc cagcccggaa ctgctgctac
     7021 gtaaagacgg cgagcgtttt agctccattc cgttagccgg ttccgcgcgt cgtcagccgg
     7081 atgaagtgct cgatcgcgaa gcgggtaatc gtctgctggc gtcagaaaaa gatcgccatg
     7141 aacatgaact ggggactcag gcgatgaaag aggtactgcg cgaacgcagt agtgagttac
     7201 acgttccctc ctctccacaa ttgattacca cgccgacgct gtggcatctc gcaactccct
     7261 ttgaaggtaa agcgaattcg caagaaaacg cactgactct ggcctgtctg ctgcatccga
     7321 cccccgcgct gagcggtttc ccgcatcagg ccgcgaccca ggttattgct gaactggagc
     7381 cgttcgaccg cgaactgttt ggcggcattg tgggttggtg tgacagcgaa ggtaacggcg
     7441 aatgggtggt gaccatccgc tgcgcgaagc tgcgggaaaa tcaggtgcgt ctgtttgccg
     7501 gagcggggat tgtgcctgcg tcgtcaccgt tgggtgagtg gcgcgaaaca ggcgtcaaac
     7561 tttctaccat gttgaacgtt tttggattgc attaaggagc gaggatgagc attccattca
     7621 cccgctggcc ggaagagttt gcccgtcgct atcgggaaaa aggctactgg caggatttgc
     7681 cactgaccga cattctgact cgccacgctg cgagtgacag catcgcggtt atcgacggcg
     7741 agcggcagtt gagttatcgg gagctgaatc aggcggcgga taacctcgcc tgtagtttgc
     7801 gccgtcaggg cattaaacct ggtgaaaccg cgctggtaca actgggtaac gtcgctgaac
     7861 tttacattac ctttttcgcg ctgctgaaac tgggcgtcgc gccggtgctg gcgctgttca
     7921 gccatcagcg tagtgaactg aacgcctacg ccagccagat tgaaccggcg ttactgattg
     7981 ccgatcgcca gcatgcgcta tttagcgggg atgatttcct caacacattt gttgcagagc
     8041 attcttccat tcgcgtggtg caactgcaca acgacagcgg tgagcataac ttgcaggatg
     8101 cgattaacca tccggcagag ggttttactg ccacgccatc acctgctgat gaagtggcct
     8161 atttccagct ttccggcggc accaccggca caccgaaact gatcccgcgc actcataacg
     8221 actattacta cagcgtgcgt cgtagcgccg agatttgtca gttcacacaa cagacgcgct
     8281 acctgtgctc gatcccggcg gctcataact acactatgag ttcgccagga tcgctgggcg
     8341 tctttcttgc cggaggaacg gttgttctgg cggccgatcc cagcgccacg ctttgcttcc
     8401 cgttgattgc aaaacatcag gttaacgtta ccgcgctggt gccgccggca gtcagcctgt
     8461 ggttgcaggc gctggctgaa ggcgaaagcc gggcgcagct tgcctcgctg aaactgttac
     8521 aggtcggcgg cgcacgtctt tctgccacgc ttgcagcgcg tattcccgct gagattggct
     8581 gccagttgca gcaggtgttt ggcatggcgg aagggctggt gaactacacc cgtcttgatg
     8641 atagcgcgga gaaaattatc catacccagg gttacccaat gtgtccggac gatgaagtgt
     8701 gggttgccga tgccgaagga aatccactgc cgcaagggga agttggacgc ctgatgacgc
     8761 gcgggccgta caccttccgt ggctattaca aaagtccgca gcacaatgcc agtgcctttg
     8821 atgccaacgg tttttactgt tccggcgatc tgatctctat tgatccagag ggttacatca
     8881 ccgtgcaggg gcgcgagaaa gatcagatca accgtggcgg cgagaagatc gctgccgaag
     8941 atatcgaaaa cctgctgctg agccatccgg cagtgatcta tgccgcactg gtgagcatgg
     9001 aaaatgagct gatgggcgaa aaaagctgtg cttatctggt ggtaaaagag ccgctgcgcg
     9061 cggtgcaggt gcgtcgtttc ctgcgtgaac agggtattgc cgaatttaaa ttaccggatc
     9121 gcgtggagtg tgtggattca cttccgctga cggcggtcgg gaaagtcgat aaaaaacaat
     9181 tacgtcagtg gctggcgtca cgcgcatcag cctgaaggag agaacacgat ggctattcca
     9241 aaattacagg cttacgcact gccggagtct cacgatattc cgcagaataa agttgactgg
     9301 gcctttgaac cgcaacgtgc cgcgttgtta atccatgata tgcaggacta ttttgtcagc
     9361 ttctggggcg agaactgccc gatgatggag caggtgatcg cgaatattgc tgcgctgcgc
     9421 gactactgca aacagcacaa tatcccggtt tattacaccg cccagccgaa agagcagagc
     9481 gatgaagatc gggcgctgtt gaatgatatg tgggggccgg gcctgacccg ctcgccggaa
     9541 cagcaaaagg tggtggatcg cctgacgcca gatgccgacg acacggtgct ggtgaagtgg
     9601 cgctacagcg cgtttcatcg ttctccgctg gagcaaatgc tcaaagagag tggacgtaac
     9661 cagctgatta ttacaggagt atatgcccac attggctgta tgaccaccgc aaccgacgca
     9721 tttatgcgcg atattaaacc gtttatggtg gcggatgcgc tggctgattt cagccgtgac
     9781 gagcatttga tgtcgctgaa atatgtggcc ggacgttctg gccgggtggt gatgaccgaa
     9841 gaattactgc cagcacctat ccccgccagc aaagcggcgc tgcgtgaggt gatcctgccg
     9901 ttgctggacg agtccgatga accgttcgat gacgacaacc tgatcgacta cggtctggat
     9961 tcggtgcgca tgatggcgct ggcggcgcgc tggcgcaaag tgcatggtga tatcgacttt
    10021 gtcatgctgg cgaaaaaccc gaccatcgac gcctggtgga agctactctc ccgcgaggtg
    10081 aaataatgga tttcagcggt aaaaatgtct gggtaaccgg cgcaggtaaa ggtatcggct
    10141 acgccacggc gctgacgttt gttgaggcgg gagcgaaagt tacaggtttt gatcaagcgt
    10201 tcactcagga gcaatatccc tttgcgaccg aagtaatgga tgttgccgac gccgggcagg
    10261 tggcgcaggt atgtcagcga ctgttagctg aaacggagcg actggacgtg ctgatcaatg
    10321 cggcgggaat tttgcgcatg ggcgcgaccg atcagctcag taaagaggac tggcagcaga
    10381 cttttgcggt taacgtcggc ggtgcgttta acctgttcca gcaaaccatg aaccagtttc
    10441 gccgtcagcg gggcggggcg attgtcactg tggcgtccga cgccgcacac acggcgcgta
    10501 ttggtatgag tgcttatggc gcatcgaaag cggcgctgaa aagcctggcg ttgagcgtcg
    10561 ggctggaact ggcgggtagc ggcgtgcgct gtaatgtggt ttcgcctggc tccaccgaca
    10621 ccgatatgca acgcacgctg tgggtgagcg atgacgccga agaacagcgt attcgcggct
    10681 ttggcgagca gtttaaactc ggcattccgc tggggaaaat cgcccgtcca caagagatcg
    10741 ccaacacgat tttgttcctc gcctctgacc tcgccagcca tatcacccta caggatattg
    10801 tggtcgatgg cggctcaacg ctgggggcat aagcatgatc tggaaacgcc atttaacgct
    10861 cgacgaactg aacgccacca gcgataacac aatggtggcg catctgggaa ttgtgtatac
    10921 ccttctggga gatgatgtgc tggaagccga aatgccggtt gatacccgta ctcatcagcc
    10981 gtttggcctg ctgcatggcg gcgcgtcggc ggcgctggct gaaacgctgg gatcgatggc
    11041 cggatttatg atgacccgtg acgggcagtg tgtggtgggc acggaactta acgccaccca
    11101 tcatcgcccg gtgtctgaag gcaaggtacg cggcgtctgc cagccgctgc atcttgggcg
    11161 gcaaaatcag agttgggaaa tcgtcgtttt cgatgaacag gggcggcgtt gctgcacttg
    11221 tcggctgggt acggcagttt tgggatgagc ccgacagaat taaatgagat tgcaggaaaa
    11281 ctcggttaac ggagtgatcg agttaacatt gttaagttaa atattggttt caactccgat
    11341 ttacatggtt accgtgttgt taaatcgtac aaagatgtta tagaaacaaa atgtaacatc
    11401 tctatggaca cgcacacgga taacaactat gaacaaatca gggaaatacc tcgtctggac
    11461 agtgctctct gtaatgggag catttgctct gggatacatt gctttaaatc gtggggaaca
    11521 gatcaacgcg ctgtggattg tggtggcgtc ggtctgtatc tatctgatcg cttaccgttt
    11581 ttatgggctg tatatcgcca aaaatgtgct ggcggttgac ccgacgcgta tgacgccagc
    11641 ggtgcgccat aacgacgggc tggactatgt gccgacggac aagaaagtgc tgttcggtca
    11701 ccattttgcg gccattgccg gagcagggcc gctggtgggg ccggtgctgg cagcgcaaat
    11761 gggctacctg ccggggatga tctggctgct cgccggggtg gttctcgccg gtgcggtgca
    11821 ggatttcatg gtgctgtttg tttctacgcg ccgtgacggt cgctcgctgg gtgagctggt
    11881 caaagaagag atggggccaa ctgccggggt gattgcgctg gtagcctgct ttatgatcat
    11941 ggtcattatc cttgcagtac tggcgatgat cgtggtgaaa gccctgactc atagcccgtg
    12001 gggaacgtat accgttgcgt tcaccattcc gctggcgatt tttatgggaa tctacctgcg
    12061 ctatctgcgt ccgggccgca ttggcgaagt gtcagtgatt ggcctggtgt tcctgatttt
    12121 cgccattatc tctggcggct gggtggcaga aagtccgacc tgggcaccgt actttgactt
    12181 taccggcgtg cagctgacct ggatgctggt gggctacggt tttgtggcgg cggtgctgcc
    12241 ggtgtggttg ctgctggccc cgcgtgacta tctctcgacc ttcctgaaaa tcgggactat
    12301 cgtcggtctg gcggtaggca ttttgattat gcgcccgacg ctgaccatgc ctgcgctgac
    12361 caaatttgtc gatggcactg gcccggtatg gaccggtaac ctgttcccgt tcctgtttat
    12421 caccatcgcc tgtggtgcgg tgtctggctt ccatgcgctg atctcttccg gcaccacgcc
    12481 gaagatgctg gcgaacgaag ggcaggcgtg ctttatcggc tacggtggga tgttgatgga
    12541 atccttcgtg gcgattatgg cgctagtttc tgcctgtatc atcgatccgg gcgtgtattt
    12601 tgccatgaac agcccaatgg cggtgctggc tccggcaggg acggcggatg tagtcgcttc
    12661 tgccgcgcag gtggtgagta gctggggctt tagcattaca ccagatacgc tcaaccagat
    12721 tgccagcgaa gtgggtgaac agtcgatcat ttcccgtgcg ggcggtgcgc cgacgttggc
    12781 ggtggggatg gcttacatcc tgcacggtgc gctgggtggt atgatggatg tggcgttctg
    12841 gtatcacttc gccattctgt ttgaagctct gtttattctg acggcggtag atgcgggtac
    12901 gcgtgctgcg cgctttatgt tgcaggatct gctgggcgtg gtgtctcctg gcctgaagcg
    12961 gaccgattca ctgcctgcta acctgctggc aacagcgctg tgcgtactgg cgtggggcta
    13021 cttcctccat cagagcgtgg tcgatccgct gggcggtatt aacactctgt ggccgctgtt
    13081 tggtattgcc aaccagatgc tggcagggat ggcgctgatg ctctgtgccg tggtgttgtt
    13141 caagatgaaa cgtcaacgtt acgcctgggt ggcgctggta ccaacggcct ggctgctgat
    13201 ttgtaccctg accgctgact ggctgaaagc gtttagcccg gatgcgaaag tcggcttcct
    13261 ggtcattgct aacaagttcc aggcaatgat cgacagcggc aatattccgt cgcagtatac
    13321 tgagtcacag ctggcgcaac tggtgttcaa caaccgtctg gatgccgggt taaccatctt
    13381 ctttatggtg gtcgtggtgg tcctggcact gttctcgatt aagacggcac ttgcggcatt
    13441 gaaagatccg aagccaacgg cgaaagaaac gccgtatgag ccgatgccgg aaaatgtcga
    13501 ggagatcgta gcgcaggcga aaggcgcgca ctaaagtcag agtgaggggg gatgttggcg
    13561 aatgttggca tagtgccgag ggttccccct caccctaacc ctctccccga tggggcgagg
    13621 ggactgaccg agtgcgttga tagcatttgt aggccggata aagcgttcac gccgcatccg
    13681 gcactctttc agcaacatgg ttagcggagg ccaagatgtt tgattcactg gcaaaagccg
    13741 gaaaatattt aggtcaggcg gcgaagctga tgattggtat gcctgattac gacaactatg
    13801 tcgaacatat gcgggttaat catcccgatc aaacgccgat gacctacgag gagtttttcc
    13861 gtgagcggca ggacgcgcgc tacggtggaa aaggcggcgc gcgctgctgc taaatttgca
    13921 cgtcaggctt taaacgattc cactttttcg aacgctgcac gcaacgtatc tggtgtcagc
    13981 gtgactggca ggtaatgaat ggactccacc gggcgcaagg tgtgggcaat cactttgtcg
    14041 atctccgcct gattattgat atccacctcc agctccgcca gcgtagtcgg cagatgaaaa
    14101 cgctgatacg ccccggttaa ttgcgccagc acatcatcct gacccagcaa ggcgctttgc
    14161 accagaattc cgtaagcgac tttggtgccg tgaagaaact tctcggtttg cggcaggaca
    14221 gtcagaccgt tatgcacggc atgagccgcc gccacacgcg taaaacgatc gcccagacca
    14281 ccgaccatcc caccaccagc aataatagca tccaccacat cgcaaaatga ttgcgttaac
    14341 tgctgatttt gctgatcggc aagtgcctgt tcgctactgt ttaacaagac gtcgcgaatg
    14401 gcctgcgcat tattgatccc cagccgcacg gttagcggca acgtttctgg ttgcggagcc
    14461 agcaccaccg cttcatacca tttcgccagc gtgtcaccga tccccgccag cagatattgt
    14521 tgcggtgcat tgaggataat ctccggttcc accagcacca taaaattggc gtcgtcgaaa
    14581 atctcataat gcagcgcctg tccggcatcg ttataccaga cggagagcgg tgtccaggct
    14641 gcgcaggtgg cggcgatcgt cggagcggca acaaatggca gaccgagacg gcgggcgagg
    14701 gctttcgcgg tgtcgagcag tgcgccgccg ccgacgccaa tcaccacgct gcgatcgtca
    14761 ccggactcag ccgccagttg ctgtacatcg ctttcgctgc aatgaccacg aaacaaaata
    14821 tgctttgccc ctggaagtcc aaacgctggc ggaagtttgg tttgcgccgc agcaatggcg
    14881 cgtttgccgt agatccacac cgcgcgagaa agttgttcat cagtgaaaaa attgtgcagg
    14941 tgattgaaac ttcctggatg tgaaaagtag ttagccgggc cgacgaccac gcggatagga
    15001 ttgtgaggca tgttgtgttg tccttattgt tttatttaga catctaaacg tcttgattgc
    15061 caaatactgg catcgtgtta tagtgccttc aacacgcaac ttcgtcaggt acaataaaaa
    15121 tgacaaataa ccctctgatt ccacaaagca aacttccaca acttggcacc actattttca
    15181 cccagatgag cgcgctggcg cagcaacacc aggcgattaa cctgtcgcaa ggctttcctg
    15241 attttgatgg tccgcgctat ttacaggagc ggctggcgta ccacgttgcc cagggggcaa
    15301 accaatacgc gcccatgacc ggcgtgcagg ccttgcgcga ggcgattgct cagaaaacgg
    15361 aacgtttgta tggctatcaa ccagatgccg atagcgatat caccgtaacg gcaggggcga
    15421 cggaagcgtt atacgcggcg attaccgcac tggtgcgcaa tggcgatgaa gtgatttgtt
    15481 ttgatcccag ctatgacagt tacgcccccg ccatcgcgct ttctggggga atagtgaaac
    15541 gtatggcact gcaaccaccg cattttcgcg ttgactggca ggaatttgcc gcattattaa
    15601 gcgagcgcac cagactggtg atcctcaaca ctccgcataa ccccagtgca actgtctggc
    15661 agcaggctga tttcgccgct ttgtggcagg cgatcgccgg gcacgagatt tttgtcatta
    15721 gcgatgaagt ctacgagcac atcaactttt cacaacaggg ccatgccagt gtgctggcgc
    15781 atccgcagct gcgtgagcgg gcagtggcgg tttcttcatt tggcaagacc tatcatatga
    15841 ccggctggaa agtgggttat tgtgttgcgc cagcgcccat cagcgccgaa attcgcaagg
    15901 tacatcagta tctgaccttt tcggtgaata ccccggcaca gctggcgctt gctgatatgc
    15961 tacgtgcaga acctgagcat tatcttgcgt taccggactt ttatcgccag aagcgcgata
    16021 ttctggtgaa tgctttaaat gaaagccggc tggagatttt accgtgtgaa ggtacatact
    16081 ttttgctggt ggattacagc gcggtttcta ccctggatga tgttgagttt tgccagtggc
    16141 tgacgcagga gcacggcgta gcggcgattc cgctgtcggt gttttgcgcc gatcccttcc
    16201 cacataaact gattcgtctc tgttttgcca agaaggaatc gacgttgctg gcagcagctg
    16261 aacgcctgcg ccagctttag ctatttaacc gtccaggcgc gggaatattg acggtcggca
    16321 aacaactctt gcaggccgtt aatttgcttc agtcgcagca cttcgtcgct gtccattccc
    16381 agctctttgc caattttatt atcatcccat cccaactggc taagttcacg gacgatttcc
    16441 gacatggcgg tgatttgatg gcgtccacga gcgcgattat gacgaatagt cgcggcgatg
    16501 cgttgattac gcgtaccttc cagacaggtc accggcaaat aaccttttaa gcgtaatttt
    16561 aatgatgatg agcctttacc aatttcatgg cggtgaaaac catccacgat ttccatggca
    16621 tttttatcc
//
LOCUS       DAJVPT010000088        16362 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVPT010000088 DAJVPT010000000
VERSION     DAJVPT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..2285)
                     /gene="rnr"
                     /locus_tag="OQM47_002881"
     CDS             complement(<1..2285)
                     /gene="rnr"
                     /locus_tag="OQM47_002881"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1480961.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKV"
     gene            complement(2324..2749)
                     /gene="nsrR"
                     /locus_tag="OQM47_002882"
     CDS             complement(2324..2749)
                     /gene="nsrR"
                     /locus_tag="OQM47_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1480962.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(2954..4252)
                     /gene="purA"
                     /locus_tag="OQM47_002883"
     CDS             complement(2954..4252)
                     /gene="purA"
                     /locus_tag="OQM47_002883"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1480963.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4356..4553)
                     /locus_tag="OQM47_002884"
     CDS             complement(4356..4553)
                     /locus_tag="OQM47_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1480964.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4635..5639)
                     /gene="hflC"
                     /locus_tag="OQM47_002885"
     CDS             complement(4635..5639)
                     /gene="hflC"
                     /locus_tag="OQM47_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1480965.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5642..6901)
                     /gene="hflK"
                     /locus_tag="OQM47_002886"
     CDS             complement(5642..6901)
                     /gene="hflK"
                     /locus_tag="OQM47_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1480966.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(6987..8267)
                     /gene="hflX"
                     /locus_tag="OQM47_002887"
     CDS             complement(6987..8267)
                     /gene="hflX"
                     /locus_tag="OQM47_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1480967.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8343..8651)
                     /gene="hfq"
                     /locus_tag="OQM47_002888"
     CDS             complement(8343..8651)
                     /gene="hfq"
                     /locus_tag="OQM47_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1480968.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8737..9687)
                     /gene="miaA"
                     /locus_tag="OQM47_002889"
     CDS             complement(8737..9687)
                     /gene="miaA"
                     /locus_tag="OQM47_002889"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1480969.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9680..11527)
                     /gene="mutL"
                     /locus_tag="OQM47_002890"
     CDS             complement(9680..11527)
                     /gene="mutL"
                     /locus_tag="OQM47_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1480970.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTMPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11537..12874)
                     /gene="amiB"
                     /locus_tag="OQM47_002891"
     CDS             complement(11537..12874)
                     /gene="amiB"
                     /locus_tag="OQM47_002891"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1480971.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(12893..13354)
                     /gene="tsaE"
                     /locus_tag="OQM47_002892"
     CDS             complement(12893..13354)
                     /gene="tsaE"
                     /locus_tag="OQM47_002892"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1480972.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13326..14873)
                     /gene="nnr"
                     /locus_tag="OQM47_002893"
     CDS             complement(13326..14873)
                     /gene="nnr"
                     /locus_tag="OQM47_002893"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1480973.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            14872..16011
                     /gene="queG"
                     /locus_tag="OQM47_002894"
     CDS             14872..16011
                     /gene="queG"
                     /locus_tag="OQM47_002894"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1480974.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(15994..16047)
                     /locus_tag="OQM47_002895"
     CDS             complement(15994..16047)
                     /locus_tag="OQM47_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480975.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(16281..16356)
                     /locus_tag="OQM47_002896"
     tRNA            complement(16281..16356)
                     /locus_tag="OQM47_002896"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16321..16323),aa:Gly,seq:gcc)
BASE COUNT         3736 a         4671 c         4116 g         3839 t
ORIGIN      
        1 tttacctttt taccgacctg acaacgcttg ccgccttttt tgcctgcatc gccttttttc
       61 gctttctcgc gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta
      121 aagtcgattt tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc
      181 aggcgataag tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga
      241 tagtagtcat tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga
      301 cggacgaaga agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc
      361 tggtcgagca tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca
      421 cgacgttccg ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg
      481 tagccgccgg tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta
      541 atggcgcggt gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag
      601 gactgcaatg ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc
      661 gagcgtagca gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc
      721 agttccgcgt agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc
      781 gccagcactg aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac
      841 agtgccggtt ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg
      901 cactcttcaa ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg
      961 cgttcagcgt tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct
     1021 tcacgggctt tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc
     1081 ggggcgtact gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag
     1141 gtcagacgcg cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct
     1201 ttcgacgaaa ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa
     1261 cacaggccgt tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc
     1321 gacgtgccac ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg
     1381 acgtcggcaa tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact
     1441 gcatcgtcaa agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa
     1501 tcgacacggc ccgcttttgc ttcttccggc acttcttctt tcagccccgc aacctgttgc
     1561 tcaacagcct gcggccagat atacggaatt tcatgggtac gcagagcgat atcaaccgcc
     1621 atgccggtgc ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg
     1681 cgagtcggac gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc
     1741 tgatcgggcg ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg
     1801 acgcccgctt cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg
     1861 acaatacgcg cttcacgacg acctttacgg tcagcaccca gcggctgcgc cagcacctga
     1921 tcgccatgaa tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct
     1981 tcaacccgca gaaagccgta gccatcacgg tgaccaataa cggtaccttt caccaggtcg
     2041 aggcgttccg gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc
     2101 atcgcgcgca ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct
     2161 accgccagct catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat
     2221 tcccgactag ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt
     2281 gacatctcgg ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata
     2341 atttataaag cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg
     2401 taagaaaact ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt
     2461 ggcaaaactc actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac
     2521 caatacgtat cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag
     2581 cagtcacgta gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga
     2641 cgccgtagac gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca
     2701 tgtagatcag cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa
     2761 aagggaaatc gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc
     2821 taatttattt cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt
     2881 aatggcttaa ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc
     2941 aggcgtacca gaattacgcg tcgaacgggt cgcgcagaat catggtttca gtacgatccg
     3001 gaccggtaga gatgatatcg atcggcacac cggtcagctc ttcaatacgc ttgatgtagt
     3061 tcagcgccgc ctgcggcagg ccgctacgat ctttcacgcc gaaagtggat tcagaccagc
     3121 ccggcatggt ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg
     3181 tagtcacttc gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc
     3241 catccagaac gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac
     3301 gacgaacggc aacggtgtcc agccagccgg tacgacgacg acgacccgta gttgcaccga
     3361 attcgttacc ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gccgggaaag
     3421 gacctgcacc tacacgagtt gagtaagctt tgaggatacc cagaacgtaa tcaacataac
     3481 gcgggcccag gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt
     3541 acggataagt accgtggtcg atatccagca gcgtaccctg cgcaccttcg aacatgacga
     3601 aatcgccacg ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca
     3661 ggatgtcggc aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt
     3721 tgtagtagtt aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga
     3781 aggtttcttt gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact ttatcttcat
     3841 aagcaggccc gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac
     3901 gcgcgttatc cagagcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca
     3961 gacgctcacg aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg
     4021 ccggagacag cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg
     4081 atggaataag atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt
     4141 gaccgccctg gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac
     4201 ctttaccttc gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa
     4261 atcaccgttt gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg
     4321 cactcaaaaa tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg
     4381 tagtagacca caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg
     4441 ggcaaattgg tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct
     4501 aaaccttcca gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat
     4561 ccttatagaa aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta
     4621 ccgcagtcgt tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg
     4681 ctatccgggc tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc
     4741 aggctacgga tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg
     4801 gctgcttcgg catcgccttc accacgcata atgcggccct gacgctcagc ttctgccagc
     4861 gttctggtca cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt
     4921 gaacggtgac gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca
     4981 gacacttcgg tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc
     5041 gccgccatac tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc
     5101 tctgccgctt cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc
     5161 gcagaaccgg agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg
     5221 acgatatctt tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt
     5281 ttcaacagca cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg
     5341 aaatcgctga tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc
     5401 acaaagcggt cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt
     5461 tcaatgaacg gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg
     5521 tcacgcagta ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag
     5581 acagacatgt aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc
     5641 gttattcccc ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa
     5701 tatcgccctg actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg
     5761 gcggcagacg cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac
     5821 ctttcagcat ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca
     5881 ctttgcgggt gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa
     5941 tttccggcgc ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag
     6001 cttccaggat ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac
     6061 cgttcgcacg cggctgaact tcgttggaat acgcttctgc ttcacgaatg tattgctgtt
     6121 cgttttcacg cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag
     6181 cagcctggaa gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt
     6241 ccagttcgcg ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca
     6301 tggtgtattt accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat
     6361 ccgggctggt cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca
     6421 tctcaacgcg cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac
     6481 gcacggcttc cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac
     6541 ccggctcaac cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt
     6601 taatggtata gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa
     6661 cgacacgacc gccaagctgc gggcgcgggc cttgtgatga actgccaccg ccagatccgg
     6721 tgcctttacc gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta
     6781 aatcaggtgg cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag
     6841 gtttgctgct tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca
     6901 tgtttgtgct ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat
     6961 gactccacgc ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt
     7021 ttacagaggc gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc
     7081 tcctccatcc actctttttc tattgcctga agctgataaa aacgactcct cagacgccct
     7141 tcctgcggtg gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa
     7201 gcctgaaaaa gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt
     7261 ttgttctctt cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc
     7321 atcaccagca gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct
     7381 tcgatgtttt cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct
     7441 tgccgcgtct cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga
     7501 ataaacccta cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc
     7561 aacgtcgggt cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg
     7621 cgattgaaaa gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg
     7681 tcggctttga tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc
     7741 gactgtatct gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca
     7801 cccggaccac gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc
     7861 aggcgcgtag ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta
     7921 cgcgcacgtt gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg
     7981 cacaaacgct ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc
     8041 gaagcaccca tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa
     8101 tactttgggt gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg
     8161 gaagagacca gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag
     8221 atgtgtacca gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc
     8281 taaatagatc agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa
     8341 ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga
     8401 tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagat
     8461 gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg
     8521 atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa
     8581 atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc
     8641 cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg
     8701 aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac
     8761 ctgtaatact tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc
     8821 ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg
     8881 ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc
     8941 cacgcaacga atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac
     9001 ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag
     9061 cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc
     9121 tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg
     9181 ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg
     9241 aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg
     9301 cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa
     9361 catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc
     9421 cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag
     9481 caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat
     9541 ccctttgtaa ataaggacag aatcaacgct tatcaactct actggtaaaa ttttacgcag
     9601 ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg
     9661 caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa
     9721 cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt
     9781 ccgccagcag ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc
     9841 gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta
     9901 tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga
     9961 tggtcacatg ctgtgcatct gactggaaat caatacccaa ttccgctagg gcagactgcg
    10021 ctttttctaa tgccgatttt tcttcgccag aaacttttag ccgcaacgga atcagcagcg
    10081 gctgggcgca aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc caacgttctg
    10141 ccactggcaa ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat
    10201 ggacgatagt cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg
    10261 gttccggcgc ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt
    10321 caccttgctg tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc
    10381 ctgaggcggg cgcaggagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa
    10441 agtgattgcg ccccgccgcc acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt
    10501 catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat
    10561 agataaaatc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta
    10621 cgttgacgtc cacctgatgc gggtcgatct ccagatacaa cacaaatgcc ggttgctgat
    10681 cggcccccag tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca
    10741 tcatgcgacc attcacgtag caatactgaa tttccgccag tgcgggcata gtgtgatttg
    10801 gatcggccac ccagccgcgc agggtgagat cgccgtgttg ccattcaatc gccagcgctt
    10861 gttcgagaaa agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg
    10921 gcactgcgcg gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga
    10981 aacgcgccag cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg
    11041 tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg
    11101 ttcccaccgg atgcgctgcc ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg
    11161 cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac
    11221 tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca
    11281 gagaggcgat tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga
    11341 taccgcagcc gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat
    11401 cgatacgcgt cgcacctgca tcgaggctgt tttccactag ttctttgact accgacgcag
    11461 gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa
    11521 ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag
    11581 tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaag taattgcgca
    11641 ggcctttgta aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt
    11701 cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgagc
    11761 gcagaacgcc aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca
    11821 actgactgat catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta
    11881 aatccagcac cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg
    11941 ccccacccag cagctccgac agtttctcgt gctgctccag ccagctggcc atttcactgt
    12001 tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactacgg ttcggtgcgg
    12061 catcagcgtg aatcgacacg aggaaattgg cgttttgctt atgtgccaca tcactgcgcc
    12121 ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcctttaaac atcggatcgt
    12181 cattgagcaa agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac
    12241 caccggggcc gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa
    12301 ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga
    12361 taacacccgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc
    12421 gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg
    12481 gtggcggcac atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct
    12541 tcaccgcttc ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat
    12601 ctttaggcgt tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca
    12661 gtccctgaat catgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc
    12721 taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt
    12781 tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg
    12841 tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg
    12901 ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc
    12961 cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt
    13021 gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa
    13081 actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgatagacc attaagttgt
    13141 cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca
    13201 gagcctgtaa aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat
    13261 agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct
    13321 gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta
    13381 acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc
    13441 gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac
    13501 ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg
    13561 ccgctcgcca tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg
    13621 accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg
    13681 gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc
    13741 tcgctaggat gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg
    13801 ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct
    13861 tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc
    13921 aggctttcgg taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc
    13981 agcagcggcg caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt
    14041 agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc
    14101 accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa
    14161 agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc
    14221 cccagtgagt caaaatgcag ttgtcctgta acatcccgcg cttttccagt gagcaagcct
    14281 agtttcagcg caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg
    14341 ccggtttcag ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa
    14401 ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg
    14461 agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc
    14521 tcgccacccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg
    14581 tcgctctcct gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc
    14641 acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca
    14701 ggatacgccg aacgacacac ctggaatgcg gcctcgccag cgcgaagcat cagctcatag
    14761 agtgtgagcc ccagcacatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac
    14821 cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag
    14881 cccctcgatc tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt
    14941 cagcaggtag gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg
    15001 ctggacaaac aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct
    15061 cgcccccatg agttattacc cggtacgctg cgtgtaatca gcgtgcggat gaattacctt
    15121 cctgctaacg ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt
    15181 tatgcgctgg gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag
    15241 atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt
    15301 ctcgagcgcc cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc
    15361 ctcaatcgcg aggccggttc gttcttcttt ttaggcgaat tgctggtcga tattccgctg
    15421 cccgtggatc aaccagtcga ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc
    15481 ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc
    15541 accatcgaac ttgaaggggc gctcccggaa gagttgcgac cgttaatggg aaaccgtatt
    15601 tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcgca actcactaca
    15661 gaagacgatt tcagcccgcg taagccgcta cacgcaccgg aactcattga gttatttgcc
    15721 tggagcgaag agaagttttt aaaagtcacg gaaggatcgg cgattcgccg tataggtcac
    15781 ctgcgctggc tgcgtaatat cgccgtggca ttaggcaatg ccccttggga tgaaacgatt
    15841 ttgacagcgc tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg
    15901 gcgattgcgc agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa
    15961 aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac
    16021 agcttgtgaa taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat
    16081 cgactcgaca aaacggaatc ttgattttta acttatttat caataggtta aaaaaatacc
    16141 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
    16201 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtagat
    16261 attttattga gagaagaatt tggagcggga aacgagactc gaactcgcga ccccgacctt
    16321 ggcaaggtcg tgctctacca actgagctat tcccgcttgg gt
//
LOCUS       DAJVPT010000089        16078 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVPT010000089 DAJVPT010000000
VERSION     DAJVPT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(113..1552)
                     /gene="rsmF"
                     /locus_tag="OQM47_002897"
                     /pseudo
     CDS             complement(113..1552)
                     /gene="rsmF"
                     /locus_tag="OQM47_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(1632..4252)
                     /gene="yebT"
                     /locus_tag="OQM47_002898"
                     /pseudo
     CDS             complement(1632..4252)
                     /gene="yebT"
                     /locus_tag="OQM47_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4221..5504)
                     /gene="yebS"
                     /locus_tag="OQM47_002899"
     CDS             complement(4221..5504)
                     /gene="yebS"
                     /locus_tag="OQM47_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1480976.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5634..6131
                     /gene="msrC"
                     /locus_tag="OQM47_002900"
     CDS             5634..6131
                     /gene="msrC"
                     /locus_tag="OQM47_002900"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1480977.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6228..6926
                     /gene="proQ"
                     /locus_tag="OQM47_002901"
     CDS             6228..6926
                     /gene="proQ"
                     /locus_tag="OQM47_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1480978.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6946..8994
                     /gene="prc"
                     /locus_tag="OQM47_002902"
     CDS             6946..8994
                     /gene="prc"
                     /locus_tag="OQM47_002902"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1480979.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9186..10067
                     /gene="htpX"
                     /locus_tag="OQM47_002903"
     CDS             9186..10067
                     /gene="htpX"
                     /locus_tag="OQM47_002903"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1480980.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10113..11486)
                     /locus_tag="OQM47_002904"
     CDS             complement(10113..11486)
                     /locus_tag="OQM47_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1480981.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            11663..12454
                     /gene="kdgR"
                     /locus_tag="OQM47_002905"
     CDS             11663..12454
                     /gene="kdgR"
                     /locus_tag="OQM47_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1480982.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12597..12836)
                     /locus_tag="OQM47_002906"
     CDS             complement(12597..12836)
                     /locus_tag="OQM47_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480983.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            12995..13138
                     /gene="mgrB"
                     /locus_tag="OQM47_002907"
     CDS             12995..13138
                     /gene="mgrB"
                     /locus_tag="OQM47_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1480984.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13213..13500
                     /locus_tag="OQM47_002908"
     CDS             13213..13500
                     /locus_tag="OQM47_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1480985.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14170..14313
                     /locus_tag="OQM47_002909"
     CDS             14170..14313
                     /locus_tag="OQM47_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1480986.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14326..14535
                     /gene="cspE"
                     /locus_tag="OQM47_002910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14326..14535
                     /gene="cspE"
                     /locus_tag="OQM47_002910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1480987.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14701..15510
                     /gene="rlmA"
                     /locus_tag="OQM47_002911"
     CDS             14701..15510
                     /gene="rlmA"
                     /locus_tag="OQM47_002911"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1480988.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15507..16073)
                     /gene="mntP"
                     /locus_tag="OQM47_002912"
     CDS             complement(15507..16073)
                     /gene="mntP"
                     /locus_tag="OQM47_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1480989.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4214 a         3902 c         4092 g         3870 t
ORIGIN      
        1 tgctctccat ttagctgctg attgtagtac gaccagtccg atcgcacaat ttttcagcct
       61 agttgaggcc ggcgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt
      121 tactggtaaa aagtttcccg tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg
      181 aaccaatccg tttggctaaa ccaatcggct gatgctggaa agtaaccaac acgtcatccg
      241 ccactggcgc ggcttgcggg taaacatcgc gcccgcgata ccactcctac gcttcctgcg
      301 gtgtcagttc aaaagcgttt acattgtcgg gggaggcatg ggcaataacc gcttcatgct
      361 gccagcgata acctttgtta tgcgtttcgg caagtttaat ccctaagcga gaaaatcgga
      421 ctttaccgat cagggcttca atgcctaccg ggaacaacca cagttctttg tcgcgttgcc
      481 agagtcgcag gtttccatcc cagtttaagc caacacttgc agccgcctga cgaatttgtc
      541 cggcttcgcg atctttcacc gggctgaacg ggaaattgcc cactttgtat tttggggcgg
      601 gtaaggcggg gatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt
      661 aaatttgtgg gaaaacatgc aaaaaacctt cttcggtcag cgccttgttt gcaccaggga
      721 agagatcgcc aagtggtaaa aactctactt cgtcggggta agtctctttc agccaccggc
      781 aaacggcttc gttttcttcc cggtttaagg tataggtcga gtaaaccagc gtaccgccag
      841 gacgtaatgc atgaaaggcg ctgtcaatca gttcccgttg ggtagctgcg atttcctgat
      901 tgctttcggg tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg
      961 agcagggagc gtccagcaaa atggcatcaa acatttctgg cactgccgca ccaaacacgc
     1021 ggccaccaaa atgtgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta
     1081 acacttttac ccaactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc
     1141 gcgcggcaat ttgcgtcgtt tttgagcctg gcgcagcagc gacatccatc acccgctgtg
     1201 gtgcattacc gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat
     1261 aaaacaggcc acttaaatgc tcggtggtac tacccaatgg caatgcatct tcattgtcgc
     1321 gttcaatcca gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag
     1381 cggttaattg caggaaatca gcaacggaga ttttcagcgt attaacgcga atgctgcggc
     1441 gcaacgggcg ctgacaggcg gcaagaaaat catcaaatga gagcgtcgaa ggcatagctt
     1501 cgcgcatttg tgtcagaaac gcgtccggga aataaacggt gtgttgggcc acgagcatgt
     1561 accactggca aaaaaacagg cgcgcagttt agcataagcg ctccggcgca ggcacgccgg
     1621 agcagtgggc attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt
     1681 tcctgcaaca ggaagtgttt gccttcttgg gctttcggtg caagtggcgt acccggaggc
     1741 gtggcgaagg cgatgccgcc acggataaat tggttaaagg tgccggtttt cactacgccg
     1801 cccgtcagac caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc
     1861 accaggtgtt gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc
     1921 cccagcgtca ttccggtaac cgtacctact tccagaccac ggaacagcac aggcgtaccg
     1981 atgcctaacg aaccggcttc cggcgcttca acaataatgc ttaaaccatc taggtaacgc
     2041 gaatcagtaa tggtggcctc ttctaattca aagtcgcgac gaggattgcc acggcctggt
     2101 tcgacgttga tatacggctg gaggatagta tctagatgct caacgccagc tgccgagatt
     2161 tgcggtgtga ccactgagaa gcgcgtacca ccgcgagcaa aggtctggac atattccgga
     2221 tagagcaccg cctttgcctg tacttcattg cgtgcggtaa tcagatccag cgtctggatt
     2281 tgcccgatat caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg
     2341 aaagcgtgaa gcgtaatctg cctgccaacc gcacgggccg ctgtttcgga agcatacaga
     2401 attcgcttgt cgcctttacg ctgactggcg ctggcaccac tgaggttatc gaagctaatg
     2461 gcccccttta atgctctgga gagcggggat gcctgtacgg tcaggccact accattcagc
     2521 tgaactttcg ccccgccttc tgcccagaac acgctgttgc tggtcaaaag gttgcgatat
     2581 tccggtttaa tgtgcagatc gatatcgaac gcgttagctc gcggacgcac ggtaataact
     2641 tcaccaactt caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc
     2701 tctgcactca aactcatggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt
     2761 tccagattgg catacagtgg atagctggct ttcatctcgc ctttatcgcc cggcagaata
     2821 cgtatcccgc cgttaatcca ttctgaggcg ctggcaccga gaaactcaac gccatccagc
     2881 cccaccttca cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttct
     2941 cgatgctgag gctcgatggc gacggtaaag gtgacgcctt tgctggtgag tttacgatcg
     3001 ataacctggc ctacctgcac gccgtgaaga atgagcggct gacccgcatc aataccgtaa
     3061 ctttccggtg ctgtcagggt cagtgtcaga acgtccggct cttgcagcag ggctttttcg
     3121 cccggcacaa caacgaactc tttgcgtggc tcgccatcgc cgggcaccaa ctcaaaggtt
     3181 tttccggtca gcagggcgct gagattagca tcgctaaggg ataatttcgg gttgcgtaat
     3241 tcgatgcggg tattttcacg aagcagggat caacggtcat ttccccagtg actttaccac
     3301 caggatttaa atccagttta gtcagctgtc cgacttccag cccctgatac attaacggcg
     3361 tcgagtcggc ggttaatcct gctccactcg gcagttccag ttttattatt actccacgct
     3421 ggctgtgggc cagatcttca tacagaccaa aggtatcttc cgcctcggca ggtttcgact
     3481 cttcaggtga atcgaaggca atcgcaccgt taaccagtgc cgccagactt tccagtttca
     3541 ccttcgcgcc actgatactg acgttggcat caacgccgga aacgtttcag aaacggctac
     3601 cttttttcac cagatcggta aaacgccgct cgatcaggac atcaatcacc acgccttgct
     3661 tgttgggatt gatggcatag tcgtagactt ttcccaccgg gatcttgcgg aaatagacca
     3721 atgaaccgct gttcagcgaa ccgagagcgg gggcttgcag gtggatcatc agatcgccat
     3781 tgtccagccg atatttcggt tgggtatcga gcgcgacaaa gtgatcctgc tctttacctt
     3841 tacccggcat catgccgata tagttcccac cgacgagggc gtccagcccg gagacacctg
     3901 ccaacgatgc ttttggcgtc atcagccaga actgagtctc ttcgcgcagc gcatctttca
     3961 tatcggactt gatgctgacc ttgacttcaa tcttacgaag atcgtcgctg aggctgatat
     4021 cctgcactgt tccgacttca acgccctgat aacgaacagg cgtacggccc ggaacaatac
     4081 catccgccga cataaagtcg atggtgacgg tattaccccg gtcctgataa ctgtcccaaa
     4141 tcagccaacc ggcaatcatt agcgcgatga aaggcagcag ccagaaaggt gagatacggc
     4201 gtttattttt aatctgcgct tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc
     4261 caaagtaagc ggctgtccag ccattccaca gcaagaatag tcaaaattac ggcagcgcca
     4321 aaataaaacg cagccggtcc catagtaaaa gcgaggatct gatcgcgatt aatcagcgac
     4381 atggttaaag atatgacaaa caggtcgagt atcgaccagc gtccaatcca ggtcaccatc
     4441 cgcagtaaca gaatgcgtgt gcgtaaacct tgctggcatt taaaatgaat gctcaacagt
     4501 aaagtgaaca tgacgatcac tttagtaaac ggtaccagaa tactggcgat aaacacgatt
     4561 cccgcaaccg caatgttgct acttgccagc gacataattc cggaaagaat cgtatcttcc
     4621 tgccggccac cattcagata aatgatagaa ataggcaata ggttggctgg taacaacaaa
     4681 acgattgacg ctaacagcgc cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt
     4741 agcgggatat ggcaacgcgg gcagagacca cgctgatcag gatagccggt aaaatggcac
     4801 ccaagacaga cacgaagttt ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc
     4861 tcccacagct cctcaacatt aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc
     4921 acaaaagaga acaagccgac acccgcctgg atatgggcat aatcctgtac ctttatagaa
     4981 gcaacgccaa tgccgaccag gtagatatcc agcattaccc actctttaag tcgctcaagc
     5041 atcagcagta ccggacgtaa attcattccc agtcggttac caaaccataa atatgctatg
     5101 gaggacacca gaatgagggg ggcaccaata acgcagaaaa agaccattga tcccgttatc
     5161 gcatcgccct gtttggtcat ttgccagatg ccttgcatga cgttggcgtc aatacgaatg
     5221 cctaacagcc agatatgcaa cagcggttcg ccccaggcaa acggcataag caacagcatg
     5281 gtgaaggcca ttgccgccag gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc
     5341 tgacagcgcg gacaataggc actttgatga gaatttatct cgggcaggct aaacagcatg
     5401 tcacattgcg ggcaacgttg gtaatcacca cgcggcagtt cctcgccgat tgccctcact
     5461 gttatctttt ttgtcggcgt aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa
     5521 taagaattaa cattaatctt aacccatgat gggaatgatc ttgagcatta acgcatttaa
     5581 tgcttatttt aataggctgg cagttccagg taagacaacc aagtgattaa gtaatgaaca
     5641 aaacagaatt ttacgcggat ttaaatcgcg actttaacgc gctgatggcg ggagaaacca
     5701 gttttctggc aacgcttgcg aacaccagtg cgttgttata cgagcgtctc actgacgtta
     5761 actgggcagg tttttatttg cttgaggacg atacactggt actcggacca tttcagggca
     5821 aaattgcctg tgtccggata cccgtcgggc gcggggtgtg cggcacagcg gttgcccgca
     5881 atcaagtgca gcgtatcgag gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg
     5941 cgagtaattc tgaaattgtt ctgccgctgg tggtgaaaaa tcagattatt ggtgttctcg
     6001 acatcgatag tactgtcttc gctcgcttta cagacgagga cgagcaaggc ttacgtcagc
     6061 ttgtggcaca gcttgaaaaa gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg
     6121 tcgcaggata atcaacggat aacgtagcat ttactggtag cgtcattata atgacgcctg
     6181 ttcatgcctg cgcttgttgg ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac
     6241 ctaagttgaa tagcagtaaa gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt
     6301 tcagtgcgga aggtgaagcg cgtccgctga aaattggtat ttttcaggat ttggtcgatc
     6361 gtgttgccgg ggaaatgaac ctgagcaaaa cgcaattacg atccgcttta cgtctctaca
     6421 cttcgagctg gcgttatctt tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg
     6481 gcaacccatg cggtgagctg gacgagcaac atgtagagca tgctcgcaag cagcttgaag
     6541 aagcgaaagc gcgtgttcag gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg
     6601 ccgcagctgc tggtgagaaa gaagacgcac cgcgccgcga acgcaagcca cgtccgacca
     6661 cgccacgccg caaagaaggc gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag
     6721 cgccaaaaac agtaaaagca cctcgcgaag aacagcacac cccggtttct gacatttcag
     6781 ctctgactgt cggacaagcc ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg
     6841 tattagaaat caccaaagac ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg
     6901 tgcgcgcaga acacctggtg ttctgaaacg gaggccgggc caggcatgaa catgtttttt
     6961 aggcttaccg cgttagctgg cctgcttgca atagcaggcc agaccttcgc tgtagaagat
     7021 atcacgcgtg ctgatcaaat tccggtatta aaggaagaga cgcagcatgc gacggtaagt
     7081 gagcgcgtaa cgtcgcgctt cacccgttct cattatcgcc agttcgacct cgatcaggca
     7141 ttttcggcca aaatctttga ccgctacctg aatctgctcg attacagcca caatgtgctg
     7201 ctggcaagcg atgttgaaca gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt
     7261 tcaggcaaac tcgacgtttt ctacgatctc tacaatctgg cgcaaaagcg ccgttttgaa
     7321 cgttaccagt acgctttgtc ggtactggaa aagccgatgg atttcaccgg caacgacact
     7381 tataaccttg accgcagcaa agcgccctgg ccgaaaaacg aggctgagtt gaacgcgcag
     7441 tgggacagta aagtcaaatt cgacgagtta agcctgaagc tgacaggaaa aacggataaa
     7501 gaaattcgtg aaaccctgac acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc
     7561 aacagcgaag atgttttctc gctggcaatg acggcgtttg cgcgtgaaat cgatccgcat
     7621 accaactatc tttccccgcg taataccgaa cagttcaaca ctgaaatgag tttgtcactg
     7681 gaaggtattg gcgcagtgct gcaaatggat gatgactaca ccgttatcaa ttcgatggtg
     7741 gcaggtggtc cggcagcgaa gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt
     7801 ggtcaaacag gcaagccgat ggttgacgtg attggctggc gtcttgatga tgtggttgcc
     7861 ttaattaaag ggccgaaggg cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg
     7921 accaagaccc gtactgtaac gttgacccgt gaacgtattc gtctcgaaga ccgcgcggtt
     7981 aaaatgtcgg tgaagaccgt cggtaaagag aaagtcggcg tgctggatat tccgggcttc
     8041 tatgtgggtt tgacagacga tgtcaaagtt caactgcaga aactggaaaa acagaatgtc
     8101 agcagtgtga tcatcgacct gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg
     8161 ctctcaggtc tgtttattcc tgcgggtccc attgttcagg tccgcgataa caacggcaag
     8221 gttcgtgaag acagcgatac cgacgggcag gtgttctata aaggcccgct ggtggtactg
     8281 gttgaccgtt tcagtgcttc ggcttcagaa atctttgccg cggcaatgca ggattacggt
     8341 cgtgcgctgg ttgtgggtga accgacgttt ggtaaaggca ccgttcagca ataccgttca
     8401 ttgaaccgta tttacgatca gatgttacgt cctgaatggc cagcgctggg ttctgtgcag
     8461 tacacgatcc agaaattcta tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaaca
     8521 ccagacatca tcatgccgac gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat
     8581 aacgcgctgc cgtgggatag catcgatgcc gcgacttatg tgaaatcagg agatttaact
     8641 gcctttgaac cggagctgct gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc
     8701 cagaacatca tgaaggatat cgcgcgcttc aacgctatga aggacaagcg caatatcgtt
     8761 tctctgaact acgctgtgcg tgagaaagag aataatgaag atgatgcgac gcgtctggcg
     8821 cgtttgaacg aacgctttaa acgagaaggt aaaccggagt tgaagaagct ggatgatcta
     8881 tcgaaagatt accaggagcc ggatccttat ctggatgaga cggtgaatat cgcactcgat
     8941 ctggcgaagc ttgaaaaagc cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa
     9001 tcaggcacaa gaaattgtgc ctgatttttt aacagcggca agatgccgta aatcagatgc
     9061 tacaaaatgt aaagttgtgt ctttctggtg acttacgcac tatccagact tgaaaatagt
     9121 cgcgtaaccc atacgatgtg ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga
     9181 aaattatgat gcgaatcgcg ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg
     9241 ggctggtact gagcctgaca gggatacagt cgagcagcgt tcaggggctg atgatcatgg
     9301 ccttgctgtt cggttttggt ggttccttcg tttcgcttct gatgtccaaa tggatggcat
     9361 tacgatctgt tggcggggaa gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg
     9421 tcaatactgt agcaacccag gctcgtcagg cggggatcgc tatgccgcaa gtggctatct
     9481 accatgcgcc ggacatcaac gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg
     9541 ctgtcagcac cggtttgctg cagaacatga gcccggatga agccgaggcg gtaattgctc
     9601 acgaaatcag ccacatcgcc aatggtgata tggtcaccat gacgctgatt cagggcgtgg
     9661 tgaacacctt cgttatcttt atttcccgta ttctggcgca gcttgccgcg ggttttatgg
     9721 gcggaaatcg tgatgaaggt gaagagagca acggcaaccc gctgatctac tttgcggttg
     9781 caacggttct ggaactggtg tttggtattc tggcgagcat tatcaccatg tggttctcgc
     9841 gtcatcgtga attccatgca gatgccggtt cggcaaaact ggttggtcgc gagaaaatga
     9901 ttgccgcgct gcagcgcctg aaaaccagct atgaaccgca agaagcaacc agcatgatgg
     9961 ctttctgcat taacggtaag tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc
    10021 tggataaacg tattgaagct ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata
    10081 ctaaaaagcg cgtcctgatc gacgcgcttt ttttatgccc tggctcgtgg ctgagtgata
    10141 cgtaaaccac tgacacaggc agcaatcact gccagaatag ccgcagccat cagcgagacg
    10201 tgcgtaccat tatcaccaaa ctgatttagc atcagcgcca ccagcgccgc gccactactc
    10261 tgacccagta gacgagccgt tcctaacatg ccactggccc caccgctacg ttcgcgcgga
    10321 gcggaggtaa taatggtgtg gttattgggt gactggaata agccaaatcc agcgccacat
    10381 aagatcatcg gccagataat attgatatcc gcaggtgacg cgggcagcag aaccagggaa
    10441 aaaagccccg tagccatgat gaacaaccct aaagcaccca gcaatcctgc atgtacgcgt
    10501 tcaatcaaat agcctgccag aggagccatc accatcgttg ctaacggcca cggtgtcaga
    10561 agtaaacctg tttcgacttc actacgcccg agcacggttt gcaggtaaaa gggcagggaa
    10621 accattgcca gcatttgtgc gcagaaagag caaacagatg tgcaaataga aagtgaaaac
    10681 agcgggatac gcagtaaatc caccggtagc agcggtacgg gaagagaaag ctggcggcga
    10741 ataaagaaaa taccaacaac aaccattacc accagttccg cagcaattaa cgtcagcgat
    10801 tgcccctgag cgaaaccact caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc
    10861 acggcgctgg gcaggtcgaa acggggttta ctggcgcgag aaccattggg tggcagaaaa
    10921 cgcatcgcca gaagcagggc gataatacct aacggtacgt tgattaaaaa taaccatttc
    10981 caggatgcga tggagaggat tgctgcagca attgtcggcc cggcagcaga agagacggca
    11041 acaataaacg agtttatgcc catccctctc cccagaaaac gttgtggata gatcaggcgg
    11101 ataagtgcgg tattaacgct catcaacgct gcaccgccga aaccttgtat gacacgcgca
    11161 agggtgagca tttgcagcga atcagaaagg gcgcagaaca gtgaagacaa cagaaaaacg
    11221 accagaccgc atttataaat acgtcgatag ccaaacatat cgcccagaaa cgaaaacgag
    11281 agcagggaga tgacaatggc gatttgatag gcgttcacta cccagatgga actggctggt
    11341 gtggcatgaa ggtccgtggc aattgttggc agggcgacgt tggcgattgc gccgtcaagg
    11401 acagccatcg aaataccaat cacaatggtt aatatcgcac cgtatcgctg gggcaatggc
    11461 aggccgtcgg cctgaacttt tggcatagga attttatatc tttggtgaat aatcaacagg
    11521 ggtatgattc tagcactgat attcacggtg gatgtcgcag aattgtaact aaccggcaaa
    11581 agatggattg cgaccaccct ggtgcgaaat tataataaaa aacagttctg atttttataa
    11641 aacactcgca atgaggtgat aaatggctaa cgcagatctg gataaacagc ctgattctgt
    11701 atcttccgtg ctaaaagttt ttggcatttt gcaggcgctg ggtgaagagc gcgaaatagg
    11761 gataaccgag ctgtcgcagc gcgtcatgat gtcaaaaagc accgtttatc gctttttaca
    11821 gaccatgaaa accttaggtt atgtggcgca ggaaggggag tcggagaaat attccctgac
    11881 cctgaaattg tttgaactgg gcgctcgcgc gttacaaaac gtcgatttaa ttcgtagcgc
    11941 agatatccag atgcgtgaga tctcccgcct gaccaaagaa actatccacc tcggcgcact
    12001 ggacgaagac agtattgttt acattcacaa aattgactct atgtacaatt tgcgcatgta
    12061 ttcacggatt gggcgtcgta atccgctgta cagcaccgcg attggtaagg tactgctggc
    12121 atggcgcgat cgcgatgaag tgaagcaaat tcttgagggc gtggagtata aacgcagtac
    12181 cgagcggacc atcaccagta cagaagcgtt attacccgtt ctggaccagg tgcgcgagca
    12241 ggggtatgga gaagataacg aagagcagga agaagggctg caatgcattg cggtaccggt
    12301 atttgatcgc tttggcgtgg tcattgccgg tttgagcatc tccttcccga cgttgcgttt
    12361 ctctgaagag cgtttacagg aatatgtcgc aatgttgcat accgcagcgc gcaaaatttc
    12421 tgcccaaatg ggttatcacg actatccgtt ctgatgagag taagaacctg tcggaatatc
    12481 aaacagacag gttctttatt tagcatgaga aaaataaagt tgaaggtgcg ttatattaaa
    12541 cgcgcttgct ataagaatat tttactcatg agtgagaatc tggttattta ttacccttaa
    12601 ccattatcga ccacgatatt gcttttacgt aacagtgggc aatctgttat ctccaagaaa
    12661 ccacttttag tgtgcaagta ttgtaccgtg ctggtgcctc tggcagtcag ataggtacat
    12721 tgcaaaccta atcctgcggc attctctttg cttccaatca aaacgccata tccgctgagt
    12781 aataatccta tccataccag tgctatcagc ataactgtgc gaatgatgaa tcgcattaca
    12841 atctcttctc tttttatgtt cgcttaatcg tagcggcaat atgcgctgaa gcaagctact
    12901 cattccgaaa aagcacgaat atcgacatag ttaggcgctg tttaactaac gcatgctagt
    12961 ttaatgacat aaggtaggtg aagcggagat tggagtgaaa aagtttcgat gggtcgttct
    13021 ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga
    13081 tcaggatgta caatttttca gcggaatttg tgccattaac cagtttatcc cgtggtgata
    13141 tcattttttc agcactagat tttcttcctc tccagcagtg gtagaatagc gcctctactc
    13201 agaggtggtg aaatgaacga agttgtaaat tcaggcgtga tgaacattgc gtctttggtt
    13261 gtatcggtgg tggttcttct tatcgggctc atcttgtggt tttttatcaa tcgtgccagt
    13321 tcacggacta acgaacagat tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa
    13381 aatgcactgt tacgtcgttt gtgcgaagca aacgaaccag agaaagcaga taaaaagacc
    13441 attgagagtc aaaaatcggt tgatgacgaa gatattattc gcctggtcgc cgaacgataa
    13501 tccttcctca atcttgagta accccgtcgt tgaagggtta ctcaattcat cctgattata
    13561 cgcactgtca tggtgcaact gcttcaaatt aaaactctgg tctgacttca aataattatc
    13621 atattattta tttgcgtaac gggatatcag aaaaagatat tttcctcatt aatttattag
    13681 gatgtttaca tcagatttgt gattaagcgt ggtattattt attagcgaaa cgtttctctc
    13741 ttgatttttt tgctcactca tcaatttttc ttattttaaa tttacaatcc ctttggggat
    13801 tgacttctct ttagggtaat tgatagccgt taactgactg ttttatgaga aaaagtgata
    13861 taacttttta ttcattgcat agcagaaaat gtgatattgc acgcactatg taataacttc
    13921 tccctctggc ctggaacaac tgaacttatt gaactatgtt agaaactacg ccagtttaag
    13981 tatctgcctg aactggcaag gttaagcaca atgatatatc ggcgcgtatt ccgttgcata
    14041 agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg tctgatcgag atatgtttaa
    14101 aaatggcttg ccataattaa cgttgtatgt gataaaagat ttcgggttaa acgaggtaca
    14161 gttctgttta tgtgtggcat tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa
    14221 taccgcttct ctgccgagcc ttatattggt gcctcatgca gtaatgtgtc agttttatct
    14281 atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt ttcaaatggc aaagattaaa
    14341 ggtcaggtta agtggttcaa cgagtctaaa ggttttggct tcattactcc ggctgatggc
    14401 agcaaagatg tgttcgtaca cttctccgct atccagggta atggcttcaa aactctggct
    14461 gaaggtcaga acgttgagtt cgaaattcag gacggccaga aaggtccggc agctgttaac
    14521 gtaacagcta tctgatcgaa tccactgatc tgaagtgtga atacgcttca atctcgctat
    14581 aaagcctcgt cgaatgcgag gctttttact atgctttatc ttcgctcctg gcgttcggat
    14641 atttgcccgc cgcgtgattc gcgttacact tgcggccttt agtatcctgc cggagttgtc
    14701 atgtcgtttt cctgtccact ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc
    14761 tgtccccaac gacatcagtt tgatatggcg aaagaagggt atgtcaatct gctgcccgtt
    14821 cagcataaac ggtctcgtga tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca
    14881 ttcttagatg ccggacatta tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa
    14941 cggcttgatg aaaaggccac ggcggtgctg gatattggct gtggtgaagg gtattacaca
    15001 cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg gcctggatgt ctcgaaggta
    15061 gcgataaaag cggcggcgaa acgctatccg caggtcactc tttgtgtcgc ttccagccac
    15121 cgtttgccgt tttccgatac cagtatggac gccataatac gtatttacgc gccgtgtaaa
    15181 gcagaagaat tagcacgagt agtgaagccc ggcggctggg tcattactgc cacgccggga
    15241 ccgcgacatt tgatggagct gaaggggctg atttacaatg aagtacatct tcatgcacct
    15301 catgcagaac aactggaagg ttttacatta cagcagagtg cggagttgtg ttatccgatg
    15361 cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg
    15421 aagccagaag tctggcaaac actggcagca aaagaagtgt tcgactgcca gacggacttt
    15481 aatattcacc tctggcagcg ttcttattaa ccgtggaagt gcgtccagag gatctggacg
    15541 ccgatgccga tcagcaccag cccgccgaga atttccgctt ttttcccaat aattgagccg
    15601 ataaagcgac caaccatcat ccctaatgtc gacataatca aggttgcaca accaatggcc
    15661 aatgcggtcg cgataatgtt gacctgaagg aaagcaagac caacacccac agccatggca
    15721 tccaggctgg tggcaatcgc ggtggttacc agtagccaga aaccgtgccg acggcgcggc
    15781 tcttcatctt catcatctgc gccacgaaaa ccctcaataa tcattcgccc gccgaggaat
    15841 atcagcagca caaacgcaat ccagtgattc cattcaagga caaaccggct ggctaacatg
    15901 cccattcccc agccgatcag cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt
    15961 cgcaatgctt cagaaaattt cggtttatgg aggggggcac ctttaccgat tgatgcagca
    16021 aatgcatcca tcgacatacc aaacgcaaga agaacagtag cagtgatatt catgacaa
//
LOCUS       DAJVPT010000090        16057 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVPT010000090 DAJVPT010000000
VERSION     DAJVPT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1933)
                     /gene="speA"
                     /locus_tag="OQM47_002913"
     CDS             complement(<1..1933)
                     /gene="speA"
                     /locus_tag="OQM47_002913"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1480990.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEE"
     gene            complement(1942..2073)
                     /gene="yqgB"
                     /locus_tag="OQM47_002914"
     CDS             complement(1942..2073)
                     /gene="yqgB"
                     /locus_tag="OQM47_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1480991.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2209..2424
                     /locus_tag="OQM47_002915"
     CDS             2209..2424
                     /locus_tag="OQM47_002915"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1480992.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2728..3882
                     /gene="metK"
                     /locus_tag="OQM47_002916"
     CDS             2728..3882
                     /gene="metK"
                     /locus_tag="OQM47_002916"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1480993.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4319..5713
                     /gene="galP"
                     /locus_tag="OQM47_002917"
     CDS             4319..5713
                     /gene="galP"
                     /locus_tag="OQM47_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1480994.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5790..6287
                     /locus_tag="OQM47_002918"
     CDS             5790..6287
                     /locus_tag="OQM47_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1480995.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6382..7089
                     /gene="endA"
                     /locus_tag="OQM47_002919"
     CDS             6382..7089
                     /gene="endA"
                     /locus_tag="OQM47_002919"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1480996.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7169..7900
                     /gene="rsmE"
                     /locus_tag="OQM47_002920"
     CDS             7169..7900
                     /gene="rsmE"
                     /locus_tag="OQM47_002920"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1480997.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7913..8863
                     /gene="gshB"
                     /locus_tag="OQM47_002921"
     CDS             7913..8863
                     /gene="gshB"
                     /locus_tag="OQM47_002921"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1480998.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8972..9535
                     /locus_tag="OQM47_002922"
     CDS             8972..9535
                     /locus_tag="OQM47_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1480999.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9535..9951
                     /gene="ruvX"
                     /locus_tag="OQM47_002923"
     CDS             9535..9951
                     /gene="ruvX"
                     /locus_tag="OQM47_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1481000.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10127..11107)
                     /locus_tag="OQM47_002924"
     CDS             complement(10127..11107)
                     /locus_tag="OQM47_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1481001.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11125..11829
                     /gene="yggS"
                     /locus_tag="OQM47_002925"
     CDS             11125..11829
                     /gene="yggS"
                     /locus_tag="OQM47_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1481002.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11847..12413
                     /gene="yggT"
                     /locus_tag="OQM47_002926"
     CDS             11847..12413
                     /gene="yggT"
                     /locus_tag="OQM47_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1481003.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12410..12700
                     /locus_tag="OQM47_002927"
     CDS             12410..12700
                     /locus_tag="OQM47_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1481004.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12708..13301
                     /locus_tag="OQM47_002928"
     CDS             12708..13301
                     /locus_tag="OQM47_002928"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1481005.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13294..14430
                     /gene="hemW"
                     /locus_tag="OQM47_002929"
     CDS             13294..14430
                     /gene="hemW"
                     /locus_tag="OQM47_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1481006.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14745..15708
                     /locus_tag="OQM47_002930"
                     /pseudo
     CDS             14745..15708
                     /locus_tag="OQM47_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15753..>16057
                     /locus_tag="OQM47_002931"
     CDS             15753..>16057
                     /locus_tag="OQM47_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1481007.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     "
BASE COUNT         3948 a         4158 c         4058 g         3893 t
ORIGIN      
        1 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcgc
       61 ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg tccgccacgg
      121 tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg aacacgtcaa
      181 ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga tatgcaccga
      241 ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
      301 tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca caggtaatat
      361 ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt agaaccggga
      421 acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa ttgacgtaca
      481 ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca cggttttgcg
      541 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
      601 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc agatccatct
      661 ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc atttcctgcc
      721 aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg gtcggcaccg
      781 tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg tgcgcagtca
      841 ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct tcacacgcat
      901 cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt acggagcagt
      961 cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg tcgaagcact
     1021 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcctg
     1081 tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt agttgcaggc
     1141 tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga gtcgcagcca
     1201 ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc tgcgaagcca
     1261 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     1321 tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc ttctcgccaa
     1381 ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg acgctacgag
     1441 tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct tccagaccca
     1501 gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta actttgatcg
     1561 gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt ttgaacgcgg
     1621 cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     1681 gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     1741 cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag tagttattgc
     1801 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     1861 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     1921 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacgactgt
     1981 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgtatgctg
     2041 acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     2101 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     2161 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     2221 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac cagaacggtg
     2281 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     2341 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     2401 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     2461 aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc cagccacgtt
     2521 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     2581 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     2641 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     2701 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     2761 tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt agacgcgatc
     2821 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     2881 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     2941 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     3001 gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga ccgtgccgat
     3061 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac taatgaaacc
     3121 gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     3181 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     3241 cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac tcagcactct
     3301 gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccaatt
     3361 ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttc
     3421 gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat cgttgatacc
     3481 tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc atcaaaagtg
     3541 gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc tgctggcctg
     3601 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacctccatc
     3661 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     3721 gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct gcacccgatc
     3781 tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc
     3841 gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt cacctgcgtt
     3901 caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat aggcgttcac
     3961 gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat cacgtttcac
     4021 ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa atatttgtaa
     4081 tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt aaagtcttaa
     4141 ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat gcccttaaat
     4201 cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct tcacatcttt
     4261 ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg agggcatcat
     4321 gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct
     4381 tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg gcgcactgcc
     4441 gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg taagctccat
     4501 gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta aactcgggcg
     4561 caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt tctctgcggc
     4621 tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg cggtgggtgt
     4681 ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa ttcgcggcag
     4741 tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt atctttctga
     4801 taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc
     4861 aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt ttgccgccaa
     4921 acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc
     4981 gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct
     5041 gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt tacaggtaat
     5101 gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct tcgaactggc
     5161 gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc tgaccaacgt
     5221 acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac caacgctaac
     5281 gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga tgcatatcgg
     5341 tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt ttattgtcgg
     5401 ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc agccgctgaa
     5461 aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca acatgatcgt
     5521 tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct tctgggtgta
     5581 tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca
     5641 cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg
     5701 cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc tcttgcagtc
     5761 atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct atcgccatcc
     5821 aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg aagctagggc
     5881 gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc ggaacagcct
     5941 ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac agtgaagctt
     6001 ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa catttcggcc
     6061 gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg ggcgttcccg
     6121 cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct
     6181 gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg
     6241 ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca cctgaactat
     6301 caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac gttgctggct
     6361 cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc
     6421 gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca ggcgaaagcc
     6481 gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg taaaattaac
     6541 tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat
     6601 gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca attcggtcac
     6661 cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc
     6721 aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa tggcgatcgc
     6781 ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca atgcgccatg
     6841 aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg tgccattgcg
     6901 cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca gcaaacgcag
     6961 ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga gcgcgatgaa
     7021 cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga
     7081 aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg cgtacaaccg
     7141 cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc atcctgaacc
     7201 actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt
     7261 actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc aggtctttga
     7321 cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg aaggccagat
     7381 cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa
     7441 aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta cgccactttt
     7501 ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc ttcagcagtg
     7561 gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg
     7621 tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga aactgaatct
     7681 tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac gcgtccgcct
     7741 gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg cccgctatca
     7801 atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc
     7861 cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa taatgatcaa
     7921 gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc
     7981 tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga tgggcgatct
     8041 gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga agcagaacta
     8101 cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat
     8161 cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct atattctgga
     8221 acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc gcgactgtaa
     8281 cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa
     8341 taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta agccgctgga
     8401 cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat
     8461 tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt acctgccagc
     8521 cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac cgtactgcct
     8581 ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga
     8641 acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa
     8701 agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga ctgaaattaa
     8761 cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg
     8821 aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct tagcgagaag
     8881 gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg gcgctgttgc
     8941 ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact ttcttattgc
     9001 catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt gcgaacataa
     9061 taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca aaattgaagg
     9121 gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc gtctggataa
     9181 accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc atactccgcc
     9241 ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca cttcccgcga
     9301 tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg ctctgggcta
     9361 tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt ggctaacggc
     9421 cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc gcgaggcggc
     9481 aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg cctgatgagt
     9541 ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt cggccaacgc
     9601 attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac gccggactgg
     9661 aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt cggtttgccg
     9721 ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt tgccaaccgt
     9781 attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag cactgtggaa
     9841 gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg caaagttgac
     9901 tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta aggcgatcta
     9961 aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac gccttatcca
    10021 gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca tcagacattt
    10081 attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac aaacgccctt
    10141 cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc tgcccggttt
    10201 gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc accgcgggag
    10261 tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact tccagctttt
    10321 gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct ttttcctgcg
    10381 ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta tgtaaggttg
    10441 ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc tcactgtcac
    10501 gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc aaccccgatg
    10561 cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc tggctggcat
    10621 agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt tgattaagat
    10681 agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc gtcaccagaa
    10741 tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca agctgttcga
    10801 gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc cgttgcgcga
    10861 acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc agctggccat
    10921 tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt
    10981 ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt gcgggccagg
    11041 cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca atttcttcca
    11101 tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc acaggtccgg
    11161 gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat tacgctgctt
    11221 gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt
    11281 caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt tcaggaactg
    11341 ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa aagccgccta
    11401 gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc
    11461 aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat taatattagt
    11521 gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc agcggcggtc
    11581 gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct tgagtcagaa
    11641 tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg actgaaaacg
    11701 cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga agccgccatt
    11761 gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg tgattactct
    11821 aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt tcaacggtca
    11881 ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct cattgtgatt
    11941 tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc gggccactgc
    12001 gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt gcctatattc
    12061 tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca atcatctgga
    12121 ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg gtcctgctgg
    12181 tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac gtgctgattc
    12241 agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg ggtgggattg
    12301 atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt gtcgcagaag
    12361 tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta tgagtgccgt
    12421 aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag ccagccgtga
    12481 ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc cgccggttga
    12541 cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg ttgccaaaag
    12601 ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc
    12661 gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg caaaaagttg
    12721 tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt agcgactttg
    12781 gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa accggcctaa
    12841 cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg
    12901 caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg ggtatttact
    12961 ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa
    13021 cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc
    13081 tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg ggcgtgatta
    13141 ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc gtaccttccg
    13201 aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc caccgtggtc
    13261 aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc gctgagtctc
    13321 tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg
    13381 ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga tctggacaat
    13441 gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg tggtacgccg
    13501 agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc gcgtttgccg
    13561 ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga agcggatcgc
    13621 tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca gagttttagc
    13681 gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa acgagcggca
    13741 aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca tggactaccg
    13801 gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact gaatccgccg
    13861 catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc gcgaccgccg
    13921 gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca gttattaacc
    13981 gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta tcagtgccag
    14041 cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg cgcgcacggc
    14101 aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt
    14161 tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga taagccgttt
    14221 gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg
    14281 tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat tgcccagggt
    14341 tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt attcttaaac
    14401 tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc gcattaatca
    14461 cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact gaataaatat
    14521 cgacgacatt tattatatat atttagtgaa tatttactat atcattcatt gattataaat
    14581 atgtacaacc tggcgcacaa atttaatatt taatttctga ctttatttca agttgtgata
    14641 aacatagaca agttgtcaga caggttaacc aatttgtcgc acaaatcact gataagcaca
    14701 attagatatc aaccaatttc catccttcaa cagggagttt ttttatgaaa atgaggtcat
    14761 ttacccgctc attagtctgc gcatccttgc tggctctggt tagcactggc gtaaacgccg
    14821 cagaaaaagt ggcgttaaag ttagcacaca acctggaaag aagtcatgtt gtacatcaga
    14881 gttttgaaga gctggcgaaa gaggtaaaac agctgtcaaa aggtaatatg gtgatccgta
    14941 tttatcccag cagccagatg ggtaatgcgc gagaaactat ggaattgttg caaaatggcg
    15001 cactggatat gaccaaaggt tccgccagtg atctggagtc ttttgataat atttatgcta
    15061 tctataattt gccgttctta ttaagttgtt tttggtgaag ttggtaaaga aattatggac
    15121 tcaacaaaag acaaaggctt ttttgcttta tcagcttatg tagcgggtac tcgcagtttc
    15181 tatgcgaaaa aaccgattac aaagccagaa gatcttaaag gactgaaaat tcgtgttcag
    15241 ccaagtccga ccaccatcaa aatgattgaa ctgatgggtg gttcacccac tccaatttca
    15301 tttggcgaag tttatactgc gatgcagcaa ggtgtagtag atggcgcaga aaataacgtg
    15361 ccttcctgga tgcagactcg acatattgaa attgccaaag ttttttctga agatgaacac
    15421 gcctcaattc ctgacttttt ggttatctcc accaaaacat ggaataaatt aaccccagaa
    15481 cagcaacaaa tcctcgaaac agcggcgaag aaatccgaag cttatcaaca aaaattgtgg
    15541 gaaaaaattg acgctgacac acgtgctcag gcgaaagcta tggggggtga aattgtaaaa
    15601 gtggataaag cgccattccg taaagccgtg caacctctgt ttgacgactt caaaaaagat
    15661 ccaaaacaag ctgctttgtt agagaaattc gacaacgctg ctcaataata ttccgggcca
    15721 gcctctggcc ctgattttcc aggatgctta caatgatatt taaccgcctg aaacttgcgg
    15781 tagaccgcgt cattgccacg ttttctgtcg ccgtaatgct tgctctggtt gtatgcgtag
    15841 tctggcaagt atttagccgc tacgtactta accagccaag tacattaacc gatgagctgg
    15901 cacgcttttt gatgatctgg gtaggattat taggtgctgc atataccgtc ggcgcgcagc
    15961 gacatctctc tattgattta tttgctctcg ccctgaataa gcgcaaacaa ttacttctta
    16021 gtatagtcat taacgttctc attttaggtt ttgccgg
//
LOCUS       DAJVPT010000091        15377 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVPT010000091 DAJVPT010000000
VERSION     DAJVPT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQM47_002932"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQM47_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1481008.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQM47_002933"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQM47_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1481009.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OQM47_002934"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OQM47_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OQM47_002935"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OQM47_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1481010.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OQM47_002936"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OQM47_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1481011.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OQM47_002937"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OQM47_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1481012.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OQM47_002938"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OQM47_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1481013.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OQM47_002939"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OQM47_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1481014.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OQM47_002940"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OQM47_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1481015.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OQM47_002941"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OQM47_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1481016.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OQM47_002942"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OQM47_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1481017.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OQM47_002943"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OQM47_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1481018.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OQM47_002944"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OQM47_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1481019.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OQM47_002945"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OQM47_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1481020.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OQM47_002946"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OQM47_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1481021.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OQM47_002947"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OQM47_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1481022.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OQM47_002948"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OQM47_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1481023.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OQM47_002949"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OQM47_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1481024.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OQM47_002950"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OQM47_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1481025.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OQM47_002951"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OQM47_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1481026.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OQM47_002952"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OQM47_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1481027.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OQM47_002953"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OQM47_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1481028.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OQM47_002954"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OQM47_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1481029.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OQM47_002955"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OQM47_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1481030.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OQM47_002956"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OQM47_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1481031.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OQM47_002957"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OQM47_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1481032.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OQM47_002958"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OQM47_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1481033.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OQM47_002959"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OQM47_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1481034.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OQM47_002960"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OQM47_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1481035.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OQM47_002961"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OQM47_002961"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1481036.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..>15377
                     /gene="rplQ"
                     /locus_tag="OQM47_002962"
     CDS             15058..>15377
                     /gene="rplQ"
                     /locus_tag="OQM47_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1481037.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGD"
BASE COUNT         3968 a         3606 c         4097 g         3706 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaa
//
LOCUS       DAJVPT010000092        15364 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVPT010000092 DAJVPT010000000
VERSION     DAJVPT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..938
                     /gene="citC"
                     /locus_tag="OQM47_002963"
     CDS             <1..938
                     /gene="citC"
                     /locus_tag="OQM47_002963"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1481038.1"
                     /translation="VTRDEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLA
                     YERHSTHLFIYTKTEYEALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHP
                     GNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTA
                     DIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFV
                     GTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKND
                     LTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
     gene            953..1249
                     /gene="citD"
                     /locus_tag="OQM47_002964"
     CDS             953..1249
                     /gene="citD"
                     /locus_tag="OQM47_002964"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1481039.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1246..2154
                     /gene="citE"
                     /locus_tag="OQM47_002965"
     CDS             1246..2154
                     /gene="citE"
                     /locus_tag="OQM47_002965"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1481040.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2165..3697
                     /gene="citF"
                     /locus_tag="OQM47_002966"
     CDS             2165..3697
                     /gene="citF"
                     /locus_tag="OQM47_002966"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1481041.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3701..4251
                     /gene="citX"
                     /locus_tag="OQM47_002967"
                     /pseudo
     CDS             3701..4251
                     /gene="citX"
                     /locus_tag="OQM47_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4226..5104
                     /gene="citG"
                     /locus_tag="OQM47_002968"
     CDS             4226..5104
                     /gene="citG"
                     /locus_tag="OQM47_002968"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1481042.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5155..6617
                     /gene="citT"
                     /locus_tag="OQM47_002969"
                     /pseudo
     CDS             5155..6617
                     /gene="citT"
                     /locus_tag="OQM47_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            6731..7537
                     /gene="rna"
                     /locus_tag="OQM47_002970"
     CDS             6731..7537
                     /gene="rna"
                     /locus_tag="OQM47_002970"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1481043.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7766..8176
                     /gene="rnk"
                     /locus_tag="OQM47_002971"
     CDS             7766..8176
                     /gene="rnk"
                     /locus_tag="OQM47_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1481044.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8407..9645)
                     /locus_tag="OQM47_002972"
     CDS             complement(8407..9645)
                     /locus_tag="OQM47_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1481045.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            9866..10294
                     /gene="uspG"
                     /locus_tag="OQM47_002973"
     CDS             9866..10294
                     /gene="uspG"
                     /locus_tag="OQM47_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1481046.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10415..11980)
                     /gene="ahpF"
                     /locus_tag="OQM47_002974"
     CDS             complement(10415..11980)
                     /gene="ahpF"
                     /locus_tag="OQM47_002974"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1481047.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12225..12788)
                     /gene="ahpC"
                     /locus_tag="OQM47_002975"
     CDS             complement(12225..12788)
                     /gene="ahpC"
                     /locus_tag="OQM47_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1481048.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13160..13906
                     /gene="dsbG"
                     /locus_tag="OQM47_002976"
     CDS             13160..13906
                     /gene="dsbG"
                     /locus_tag="OQM47_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1481049.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14116..15018
                     /locus_tag="OQM47_002977"
     CDS             14116..15018
                     /locus_tag="OQM47_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1481050.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15165..15364
                     /locus_tag="OQM47_002978"
                     /pseudo
     CDS             15165..15364
                     /locus_tag="OQM47_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3745 a         4013 c         3768 g         3838 t
ORIGIN      
        1 ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat attattaaat
       61 gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta gccactgaac
      121 tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc aaaaccgaat
      181 acgaggcgct gttccgccag tgcggttttt ccacgctgac ctgcgtaccc ggcgtgatgg
      241 tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg aaaaaatttc
      301 gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt acgaatggtc
      361 accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg tttttagtca
      421 aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta aaaggcaccg
      481 ccgatattcc acgcctgact gtgcatcgcg gctcagaata catcatctcc cgcgctacgt
      541 tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc gaaattgatc
      601 tgaagatttt ccgtcagtac ctcgctcccg cgcttggtgt aactcaccgc tttgtcggta
      661 ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac tggctggaaa
      721 cgccgactat ctccgcagcg cccatcgaac tggttgaaat tgagcggctg cgttaccagg
      781 agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat ctcacggcta
      841 tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt gagcactccc
      901 gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa aaatgaaaat
      961 aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac gcatcgcccc
     1021 actcgatacg caggatatcg acctgcaaat caatagcagc gttgagaaac agtttggcga
     1081 tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg gcgtacagct
     1141 gaatgtcgat gacaaaggcg cactggactg cattttacat gcacgactgg aagccctgct
     1201 ggcacgcgcc agcggtatcc cggctctgcc atgggaggat tgccaatgat ttccgcttcg
     1261 ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg tgccaatgcc
     1321 gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga cctcgaagac
     1381 tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc gctgcaacat
     1441 ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc cgaatggggt
     1501 gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct gccgaaaacc
     1561 gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtataga aaaagcctgt
     1621 ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct gggcattacc
     1681 cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa
     1741 gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact gctgttcgca
     1801 cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga taccgtctat
     1861 tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa acagctgggc
     1921 tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa cctctacgca
     1981 ccgacccaga aagaagtgga tcacgcccac cgcgtcgtag aagccgctga agccgccgct
     2041 cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc ggttatcgat
     2101 cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga ataaggcaat
     2161 caaaatgacg cagaaaattg aacaatctca acgacaagaa cgggtagcgg cctggaatcg
     2221 tcgcactgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct accaggctga
     2281 aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc gctcaggttt
     2341 acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa
     2401 tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgaccctgg cgtccagctc
     2461 cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg ttacccgcat
     2521 ttatacctct ggcctacgcg gtccactggc ggaagagatc tcccgtggtc tgctggcgga
     2581 accggtgcag atccactctc acggcggtcg tgtgcatctg gtacagagcg gcgaactgaa
     2641 tatcgacgtg gctttcctcg gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta
     2701 caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa
     2761 acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg caagcattga
     2821 gcaagatcag gttgatttga tcgtcaaagt taatcgcgtt ggcgatgctg caaaaatcgg
     2881 cgctggtact acccgtatga ccactaaccc gcgcgaactg cttattgccc gtagcgctgc
     2941 ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa ccggcaccgg
     3001 cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc gcgatattcg
     3061 cgccgacttc gccctcggcg gtattaccgc gacgatggtt gatctgcacg aaaaaggtct
     3121 gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat cgctggcccg
     3181 taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt cgaaaggcgc
     3241 atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca ggcagttcaa
     3301 cgttaacgtg ctgaccggtt ctgacggcgt actgcgtggc gcttccggtg gtcactgcga
     3361 taccgcgatt gcctctgcgc tttccatcat cgtcgcgcca ctggtacgcg gtcgtattcc
     3421 gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg atattctggt
     3481 cacagaccac ggtatcgcag ttaacccggc acgtccggaa ctagcagaac gtctgcagga
     3541 agcgggcatt aaagtggttt ccattgagtg gctgcgcgaa cgtgcgcgtc tgctgaccgg
     3601 tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg
     3661 ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc ttcctgaact
     3721 cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg aaaggcaagc
     3781 acggcaacac gcctggctca agcaccatcc tgttccactg gtctccttta ccgtggttgc
     3841 gcctgggccg attaaagaca gcgaggtcac acgccgaatt tttaatcatc gcgtgacagc
     3901 cttgcgtgcc ttagccacaa agcagggctg gcaaattcag gagcaggctg cactggtttc
     3961 cgccagcggg ccggagggca tgttgagcat tgccgccccg gctcgcgacc tcaagctcgc
     4021 caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg atgtcctgac
     4081 gcccgaaggc gaaattctct cccgccgcga ctattcactg cgcctcgccg ctgcctgttg
     4141 tgcgagcaaa gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc
     4201 aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa
     4261 gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga
     4321 agtcaatctg tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga
     4381 tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt
     4441 cattgaattt ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg
     4501 cccaattggt atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca
     4561 taaaggcagc attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct
     4621 caaccagccg gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct
     4681 gaccgatcgc gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca
     4741 gcagcttggc cttaccggcg cacgcggtga agccgaagca ggttatccac tggtgatcaa
     4801 ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt
     4861 gctcgatacc ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg
     4921 tggcgagggg ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg
     4981 cattcgaacc cccaccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg
     5041 aaatctcagt ccaggcggca gtgccgacct gctgatcctt acctgttttt tagcacagat
     5101 ttaattattt aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct
     5161 ttagcaaaag ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt
     5221 cttatccctg tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg
     5281 gcaatgattg tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg
     5341 gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac
     5401 ccagcgttta atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc
     5461 acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta
     5521 ggtcgtcgca ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt
     5581 tatgcgattg tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt
     5641 accgggggta cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg
     5701 aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc
     5761 agtctgagtt cgtccatgtt tgtcaccggt gcggaccaaa cgtgctgggt ctggagttcg
     5821 tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg
     5881 ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa
     5941 tcacacacag tgaagaagtg gcaacctggg cgggtgatga actaaaaacc atgggtgcgc
     6001 tgacacgcag agagtggacg ctgattggcc ttgtattgct cagcttaggt ttgtgggtat
     6061 ttggcagtga agtcattaat gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg
     6121 ctctgcacgt tgtgccgtgg aaagacatta cccgctataa cagcgcatgg aacacgctgg
     6181 tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggt tttattgact
     6241 ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca acggtgattg
     6301 tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg
     6361 caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac
     6421 aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa
     6481 ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc
     6541 ttggcgcaat cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc
     6601 tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta
     6661 agcccacctg gcgggctttt ttatgattta atagatagta ttcagtgtat gtatgagttc
     6721 cacacccatt atgaaagcat tctgccgtaa cgccgcgttg ctcgcggttt ctctgcttcc
     6781 cttatcttct gccaacgccg tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta
     6841 tgtcctggcc ctctcctggc aaaccggatt ttgccagagt caacacgatc gaaatcgtaa
     6901 cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac aaaactgatt ttctgaccgt
     6961 acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg
     7021 ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cacgcgccag
     7081 ccgaatgtgt tcatcgccgg aaaccgggtt atcactggaa acggccgcta aactaagtga
     7141 agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg
     7201 tgcctgcttt ggttttgatc cggacgcata cttcggtacg atggtacgcc tgaatcaaga
     7261 aattaaagag agcgaagccg gaaaattcct tgcggataat tacggtaaaa cagtgagccg
     7321 ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa
     7381 gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcga tcaaagctga
     7441 cgccatcaac gctccgcttt ctgcaaactc attcttgcca caacctcacc caggtaactg
     7501 tggcaaaacc tttgtgattg ataaagcggg ttattaatcc tctgatttac aaaacacgct
     7561 aattcgtctt ccataaccat gccgattaag aattatacat aatgcgctgc attgaggaat
     7621 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
     7681 ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc
     7741 gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga
     7801 tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga
     7861 cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga
     7921 cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt
     7981 gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc
     8041 tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc
     8101 gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg
     8161 cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac
     8221 tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg
     8281 caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata
     8341 ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga aaaccactcc
     8401 tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc
     8461 cgcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt
     8521 ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc
     8581 ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac
     8641 gtgggtctgt cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc
     8701 gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc
     8761 aatacactgg cgcaacgctt taccgctgct gccctccagt ttcaggttag tcagcaccgt
     8821 ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct attactgcat ccacgccccg
     8881 gtgacctgcc gtttgttcaa taattgactg tgccggatcg ctatcttcat cgaaattaat
     8941 cgggatcgcg ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat gatcaacaac
     9001 aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc ccacaggacc
     9061 agcaccatag actgcaacgc ttgaaccttg ttggatctgc acattttttg ctgcctgcca
     9121 tgccgttggc agaatatcag aaaggaaaag ggctttatca tctgaaagca aaggcggtac
     9181 tttaaacggc cccacattcc ctttcgggac gcggacatat tccgcctgcc cgccaggaac
     9241 gccaccatac aggtgactat aaccaaacaa tgccgcggga gcaggtatct gttttttatt
     9301 gagcgcagcg cctttacccg cattggtatt ttcgcaggcg gcatattgct gtaatcgaca
     9361 gaaaaaacag tcgccacaag caatgacgaa cggaattacc acccggtcgc ctttttgcaa
     9421 attttttacg tccttcccgg tttcaacgac ttcccccata aattcatgac caaaaatatc
     9481 gccatgttta acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc
     9541 cgttaccgta atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt
     9601 ttctacctga acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac
     9661 agttcaaaaa agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg
     9721 caggttttgg gagaattcct agtgttcagt gagaatatat taatcacaga aaccgttcgt
     9781 aaggaaaaag aaccagacaa atatcaggga taaatattat aatcatggag aatgatttgt
     9841 tcatgattaa cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt
     9901 tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg
     9961 agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc
    10021 cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac
    10081 gatggtcagc cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag
    10141 cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg
    10201 ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg
    10261 ccacgccaat ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg
    10321 cctggaacat cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg
    10381 cggctaagca attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt
    10441 agtcaaaagc actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt
    10501 acggaaccgt cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg
    10561 catcaatgat aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt
    10621 tcggcagcag accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc
    10681 tgacacgatc tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg
    10741 tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct
    10801 ggtcggcttt catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca
    10861 ggtcgattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt
    10921 taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt
    10981 cgcccggaac gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt
    11041 tcagtaccgc gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga
    11101 tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt
    11161 tcagtgcgcc tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat
    11221 caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt
    11281 tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt
    11341 cataagcatc acgcttgttc agctcttctg ccgcacgatt ttccgcgcca gtatcaattt
    11401 tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca
    11461 ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa
    11521 ttgcagtgtg cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt
    11581 ccgggcagtt atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat
    11641 ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca
    11701 acgccagtac cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct
    11761 ggttggaacc tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt
    11821 ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg
    11881 ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa
    11941 ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa
    12001 acatcatcat gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg
    12061 aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc
    12121 agagcaaggc gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga
    12181 gcgggcgacg ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc
    12241 aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca
    12301 cctgggtgag aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca
    12361 cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg
    12421 aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg
    12481 gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg
    12541 tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt
    12601 ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta
    12661 aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt
    12721 tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc
    12781 aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga
    12841 ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac
    12901 aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt
    12961 gaccgtgtaa tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt
    13021 gcccttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac
    13081 ctgatctaag ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact
    13141 gactgaaaag aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat
    13201 cgccttcgca gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat
    13261 catcaaaacg ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat
    13321 gggcgtcacc atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa
    13381 cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg
    13441 acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc
    13501 gccggtcatt gtctacgtct tctccgatcc gttctgccca tattgtaaac agttctggca
    13561 gcaggcgcgc ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt
    13621 tatcaagcca gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa
    13681 aacctggcaa caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt
    13741 aagtacagag caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc
    13801 aaatgtcacg ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg
    13861 gttgcccgat cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg
    13921 atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt tctcgctcta
    13981 ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat
    14041 caataaaaat acatacgcaa aaaacattga ttaagtgaat ataccatgga agaaaaatat
    14101 aaaccggagt agtgtatggc caatctctac gacttgaaaa agctcgactt aaatcttctt
    14161 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
    14221 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
    14281 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac
    14341 catcttgaga aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa
    14401 ctaaagaaaa actttattat ctacggccct cagctcattt cttgctctaa taatagtatg
    14461 ctgatccgct gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg
    14521 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
    14581 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta
    14641 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
    14701 gaagagttca cccaattaat agccaaatca gcaggagttg atgatattca aatggaaata
    14761 gacgagaagt ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt
    14821 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taactaccac
    14881 cgtgactttc ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt
    14941 tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt
    15001 ctaaatgaga gtttttgata tataactata gacagctact ataactttca tctatttatt
    15061 cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt
    15121 cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
    15181 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca
    15241 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
    15301 tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt atcaactggg
    15361 aggc
//
LOCUS       DAJVPT010000093        14998 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVPT010000093 DAJVPT010000000
VERSION     DAJVPT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(169..1194)
                     /gene="tdh"
                     /locus_tag="OQM47_002979"
     CDS             complement(169..1194)
                     /gene="tdh"
                     /locus_tag="OQM47_002979"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1481051.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1204..2400)
                     /gene="kbl"
                     /locus_tag="OQM47_002980"
     CDS             complement(1204..2400)
                     /gene="kbl"
                     /locus_tag="OQM47_002980"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1481052.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2675..3532)
                     /gene="yibB"
                     /locus_tag="OQM47_002981"
     CDS             complement(2675..3532)
                     /gene="yibB"
                     /locus_tag="OQM47_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1481053.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3836..4768
                     /gene="rfaD"
                     /locus_tag="OQM47_002982"
     CDS             3836..4768
                     /gene="rfaD"
                     /locus_tag="OQM47_002982"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1481054.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4778..5824
                     /gene="rfaF"
                     /locus_tag="OQM47_002983"
     CDS             4778..5824
                     /gene="rfaF"
                     /locus_tag="OQM47_002983"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1481055.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5828..6820
                     /gene="rfaC"
                     /locus_tag="OQM47_002984"
     CDS             5828..6820
                     /gene="rfaC"
                     /locus_tag="OQM47_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1481056.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6817..8025
                     /gene="rfaL"
                     /locus_tag="OQM47_002985"
     CDS             6817..8025
                     /gene="rfaL"
                     /locus_tag="OQM47_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1481057.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8061..9203)
                     /locus_tag="OQM47_002986"
     CDS             complement(8061..9203)
                     /locus_tag="OQM47_002986"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1481058.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9212..10225)
                     /locus_tag="OQM47_002987"
     CDS             complement(9212..10225)
                     /locus_tag="OQM47_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1481059.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10250..10957)
                     /gene="rfaY"
                     /locus_tag="OQM47_002988"
     CDS             complement(10250..10957)
                     /gene="rfaY"
                     /locus_tag="OQM47_002988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1481060.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10983..11990)
                     /gene="waaO"
                     /locus_tag="OQM47_002989"
                     /gene_synonym="rfaI"
     CDS             complement(10983..11990)
                     /gene="waaO"
                     /locus_tag="OQM47_002989"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1481061.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12033..12830)
                     /gene="rfaP"
                     /locus_tag="OQM47_002990"
     CDS             complement(12033..12830)
                     /gene="rfaP"
                     /locus_tag="OQM47_002990"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1481062.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12823..13947)
                     /locus_tag="OQM47_002991"
     CDS             complement(12823..13947)
                     /locus_tag="OQM47_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1481063.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13944..>14998)
                     /gene="rfaQ"
                     /locus_tag="OQM47_002992"
     CDS             complement(13944..>14998)
                     /gene="rfaQ"
                     /locus_tag="OQM47_002992"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1481064.1"
                     /translation="KPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDT
                     IPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAILV
                     RLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQTTM
                     SYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVL
                     TSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAA
                     AVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVI
                     AAVDKLLPSSTTGTSL"
BASE COUNT         4264 a         3371 c         3086 g         4277 t
ORIGIN      
        1 gagatattat ttttacaggt gagagagtca cttcagggta gagagataat gagaacagct
       61 atataaaaga tcagcgcaat tacgaactct ttattacact gacaggattt tacataatta
      121 acagattatc ctcagataaa agggctggaa taccagccct tgttcgtgtt aatcccagct
      181 cagaataact ttcccggact ggcccgaacg catagcgtca aagcccttct ggaaatcatc
      241 gatagagaaa cgatgggtaa tgatcggcga gagatcgagg ccagactgaa tcagcgccgc
      301 catcttgtac caggtttcaa acatctcacg accgtaaatg cctttaatga acaagccttt
      361 aaagatcact ttggtccagt cgatagacat atcagacggt ggaataccca gcatcgcaat
      421 acgaccgccg tggttcatgg tatcgagcat ggtacgaaac gctggcggcg cgccggacat
      481 ttccagaccg acatcaaagc cttcggtcat gcccagttca gccatcacat cattaaggtt
      541 ttctttcgcg acgttaaccg cacgggtgat acccattttg cgcgccagct caaggcggta
      601 ttcgttaaca tcggtgatca ccacattgcg tgcaccaacg tgtttcgcca ccgccgcagc
      661 cataatacct atcggacctg caccagaaac cagcacatct tcgcccacca gatcgaacga
      721 cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt cgtcggagat
      781 attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatattcgg cgaagcagcc
      841 cggacggtta acaccgacac caatggtgtt gcggcacaga tgggtacgac cgccacgaca
      901 gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc cgattttgaa
      961 gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc ccacgaccat
     1021 cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa cgtcagtccc
     1081 gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt ccgatacagg
     1141 gacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt tcatctcaca
     1201 tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta ctgcacgcgt
     1261 aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct gacctttcgg
     1321 aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcagct ctcgggcaaa
     1381 tttctgcgcc actaccgcat caccaagcat gaccggaata atggcgtgat cggctcccgc
     1441 cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg cccacagacg
     1501 gtcacgcaac tcgctacccg cttcaaccat ctccagcact ttgatggaag cggcgacaat
     1561 tgctggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca gccactcaac
     1621 cacttctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt taccgagcgt
     1681 gccggtgatg atgtcgacac gccccatcac atcgcagtat tcatgggaac cacgaccatt
     1741 ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat cgtatttatc
     1801 tgccagatcg caaacgccct tcagattggc aatcacgccg tccatcgaga acacaccatc
     1861 ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac gagcttcaag
     1921 ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac gcacaccatc
     1981 aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc ccagcagcgt
     2041 ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat cttccatccc
     2101 caggaatgcc gccagttttt gttcaagctc tttatggctg tcctgagtgc cgcaaataaa
     2161 acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgctg ccgcaatcag
     2221 atccggatga ttcgccagcc cgagatagtt gttggcacaa aagttaatga cgtggcttcc
     2281 atcagccacg gtgatatctg cttgctgcgc agacgtaata atgcgctctt ctttaaacaa
     2341 cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt ctccacgcat
     2401 tgcgattctc cagacagggc aaattccagc acatattacc caaacttata ggtcgcgacg
     2461 agatgatgcg ttaacacttc tgcaaaattc aggataaata acgtgtatct tgatgtctct
     2521 caacacctac agatagtaga aaaactgtac agttttggct gagcataatg atgagtgatt
     2581 ttactgcatt gatataaaaa gaaaaaatga tgccaaagca tcatttttcc tatctcagtt
     2641 ggcatcaaaa cgctacccac taatggaggg aaacctattt tcttgaaagg aaaattctca
     2701 gcgcctgcat ttttgcccct aaagtgttac tcctgaagca gcgaaacaaa tcgaaccatt
     2761 ttcctctccc cagatagttt aggttgaaga gatcggaacg tttgtaatag cacatgacaa
     2821 aaatgccctg gtcatcgtcc acaatgttat tgtttagtgt tattttttga ctttccagaa
     2881 ctaatttata gaattctttc catttatgct gtgaaccaac aattgcgccc ccaataatat
     2941 atacatgatt gccaatcatg aaatcaaaag cctgttgttg agatgtaaca gtaagccctt
     3001 tttttatagt gaaaagatgc atcttatttt catcaaatgg gaaatcccaa attttcaatc
     3061 ctcgagttac atttggctta cggcaatagc caaaatctat ccacgcaaca agaggcgttt
     3121 tcactaaacc catgttgata gctttattta caaaataggc cttaaggtta cataccaaaa
     3181 catactctgg tgaccagtac tctgggtttt ttaattgtcg aggctcaagt ctatttgtaa
     3241 atgactcatc tttttgaatt ttttcgatac gacttctgat atatctaaat ttctttttta
     3301 tatcgataac gataaccgtt gttggttttc cgttgcgaat cgcttcaatc cttggtttca
     3361 aatcgggaga agtgaaaata atcatctcat tttcaagtgc ggccagacgc tcaaagtaac
     3421 taaaataaac atcaactgaa cgagctagtt tttcacggaa ccctttatta gcagtccaat
     3481 cccctctacc aatgtcaaaa tatgcagtga taattgtggt ggatgatttc attactaata
     3541 agaccattaa tataaaaaaa gaattatatc ataaatatta acagaatgta tactgtatat
     3601 tatccattca aatgattccg ttatttatca tacatcggaa tattgatact aaagcactat
     3661 tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc
     3721 aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat
     3781 tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat
     3841 catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaggccc tgaatgataa
     3901 aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct
     3961 ggtggatctg gatatcgcgg actatatgga taaggaagac ttcctgatcc agattatggc
     4021 tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa ggcgcatgct cttccaccac
     4081 cgagtgggat ggcaagtata tgatggataa caactatcaa tactccaaag agctgctgca
     4141 ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct tccgcagcca cctacggcgg
     4201 acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaatg tctacggtta
     4261 ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg ccagaagcga actcgcagat
     4321 tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc
     4381 gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga agctgttcga
     4441 aggtagcgag aacttcaaac gtgacttcgt ctatgtgggc gacgtggcag atgtaaacct
     4501 gtggttcctg gaaaatggcg tttccggtat cttcaacctc ggcaccggtc gtgcagaatc
     4561 cttccaggca gtagcagatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat
     4621 tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa
     4681 tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata
     4741 catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc
     4801 gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta
     4861 tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat
     4921 gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga
     4981 acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc
     5041 aaactccttc aaatctgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg
     5101 gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg
     5161 gccgctaatg gtggaacgct atgtcgcgct ggcctatgac aaaggcatta tgcgtaccgc
     5221 acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc
     5281 atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggct tttgcccggg
     5341 tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggaac tggcaaagca
     5401 gctgattgat gaaggttatc aggtggttct gtttggctct gcgaaagatc atgaagcggg
     5461 caatgagatt cttgccgctt tgaataccga gcagcaggca tggtgtcgga acctggcggg
     5521 ggaaacacag cttgatcaag cggttatctt gattgcagcc tgtaaagcca ttgtcactaa
     5581 cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg
     5641 tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat
     5701 taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat
     5761 cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc
     5821 ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt
     5881 gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga
     5941 agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc
     6001 aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt
     6061 tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa
     6121 aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac
     6181 cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca
     6241 gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca
     6301 aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg
     6361 cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca
     6421 ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg
     6481 cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt
     6541 attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt
     6601 gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt
     6661 ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg
     6721 ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct
     6781 tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt atttttctct
     6841 ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc
     6901 acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct tatgacaata
     6961 acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt taaaacattt
     7021 ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct tcaatcccct
     7081 gatgcaggtg ctagcatgaa ggaagttttc aaagctatta ttgagaatac tttactatgc
     7141 acaatagcaa taccggtcat attgagagac gagaaaagag aagatgtcga aaaaatcgtt
     7201 ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat tacctattat
     7261 aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag catttctgac
     7321 tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc agcaaaatac
     7381 cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg aactttatcc
     7441 agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat gtttaaacaa
     7501 tggaagttac tattagtagg agtaatggtt tccatcattg cattgtcggt tattttcaca
     7561 cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag ttcttatcgc
     7621 tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc tgttattggt
     7681 tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat tgattatcca
     7741 gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat ctggtttggc
     7801 actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt gaaagagtgt
     7861 ataaaaaatg gcgtcaagaa taaatatcgc tcaccatata atgcatatta tataatctta
     7921 ctatctttta taggttattt tgttatccgt ggaaacgtag aacaaattga accaaattta
     7981 ttaggcgttt acgccggctt attattagcg atgaaaaaca agtaaaaaat aaaaaaggct
     8041 gcataatgcg gcctttttat ttattcaaac caattatgaa taacctcttc gaatctttga
     8101 gttacgccat cccaactgta gtgatcaaac acaaagtcct gaccttgctt tgcaactgct
     8161 gttaattcag gattcgctaa cgtttttaaa atatcgctgc ttatggagtc tgcagtcata
     8221 ggttctttta aatggaatcc cgtagtattt tctttgacaa attccgtcat ccccccacgc
     8281 gtactcacta aaacaggctt accagcaccc atagcttcaa ttgccaccat gcaaaatggt
     8341 tcctggaact gggaaggaat gacgactaaa tctgccagtg ggtaataaca atacatcttt
     8401 tcaggcggta tgcttcctaa cataatgcaa cgatccttca aacgttttgc aatttccctg
     8461 acattgcgtt gatatgcacc tttgtcactt ttactcattt ctgtataatc accaatcaca
     8521 actaatttta aatttttgtg agcagcggcc aatttttcga atgattgcat caacagcaat
     8581 atgcctttgt cagggacgat acgcccagca taaaaaataa ttttctcttc aggagagata
     8641 ttaagttcgc ttctagtgat cggctgaaaa ttagattgat aggtttctaa atcaattcca
     8701 tttggtacaa tctcaatatc tgcatttgct aaataagatt ggtaatactt tttcaagtat
     8761 aaactaggga ctatcatttt aacattttgc tcaagcaatt ttggttcaaa tgcattatgc
     8821 atatgtataa ccactcttgc ctgaggagct agcttacgaa tttgagtgta tagcttcatg
     8881 ctattatgaa ctacaataac actttctttc gttataggaa aatcatgtgc aatattcaat
     8941 attctctgtg aatatggaag aggatcaagt cttgtccatt tttgaaataa ccgcttatat
     9001 atgcgactga atccaaccct atgaatacta caattatcat taacaaaggt gtaatttgag
     9061 tatcctggat ttttgataca aacaatacga tttggaaaat tggtacgctg agcaacctga
     9121 tacatccacg tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat aggagtaacc
     9181 gtaaatatta ttttatcaac catctcgtat attaaccttt cataacatta tatttaattg
     9241 ctgtaaaaac accatcgaga aattttttct ggtaaagcaa gtgtttatat ttttctttgt
     9301 gctcatgttt tttaacagct tttttataag gtatatttct ccatggtgat atattataaa
     9361 tattacgaaa ataatctgca gaggcgtagt tcgcccaatc atgccatggc ttagttatac
     9421 cagtataatg tatgaagaca gtgtcatcat taataaaccg ggtgtaatat tcactatttt
     9481 tttcttcaaa ttctgacttt atagtataaa tacaattata ttttctcggt aatattttag
     9541 catgatgtag aaacattaag tttaaaatat cttgatccgg gtactttaat ttttttataa
     9601 ttgactcatc tgataataga tcaaagaatt tttccgttag tctttgtttt aaccattccc
     9661 gtaaattaat atacattaca ccagagttaa aatagctacc gttaaaatcc tcattacaca
     9721 aacgctcagc gcttttactt tgcatagcat ctacatcaat taccactgcc ccatattcat
     9781 ctttaaactc taatgctatt aactcgttca atgaaccctt acagacgata tcagcatcaa
     9841 gatataataa tgaatccaat cgcgcagaaa aataatcaaa tgaaagcaaa cgataataaa
     9901 tagatactgg ccagatattt gattgaggta gcgccttcaa tggctcagaa tcaatatgat
     9961 ataatttaat tactgtttga tacttcgttg ctaattgtga taataattca atatactctt
    10021 tgtctaagta atcagcgatt atgtgaaagg taaatttgtt ttgtttattc ttttcaagaa
    10081 ttgaagcaat tgaaatcgca gccccaacct ggtagttctc atcaataccc catgagacat
    10141 taaacgtttc ccgttcatca agtttaacgg ggcgcttgtc cagttcaatt atatctttaa
    10201 attgagtaag atgtttaaaa tccaatttaa ttacccttca tcataatcat tatttgctct
    10261 tcaatcctag tttgactttt acatccctga ttactttttt aattttcttt ttgaatataa
    10321 gatatgtata gccaaagtct ttcaattcat tttttatatt atagtgccgt tcaagatcaa
    10381 tgcgatcttt tgcttttaaa acagcggttg tttttttacc tgaaaggtca attagtctta
    10441 aacctttttc ggatactata aaatttcctt tatgaggatc gccagacacc attccatgct
    10501 gatgcaactc ttttatcgat tctgataatt gatccttcag atcttcagaa atttccaaat
    10561 actcatttaa cccgacacct tcaatatact caatgagcat aatataataa tgagcgaaat
    10621 ttaatgtttt acgttcagct agcaaaaaat agtcattaat tgattggatg ccttcaccac
    10681 gtacccgatc ggtttgataa attaaatttt cataataatc tttttttatg caagatttta
    10741 agaaacgttc ggtcatttta tgctttggcg cataaacttt aagtacaaga ggtccacgag
    10801 cagtgtcaat aagaatgact ttagtatcat caatagaacg aaaaactttt aatgttttaa
    10861 ccctgcaagt taaaaagtca tttagaactt gctcataaaa gggatcatta tcttttatga
    10921 aaactttcaa cccatttatc gttttattat atatcataat gaattatttt aaccttaaat
    10981 ctttagaagc atttttcttt ataatacttt aaataattaa aaatgcctgc catataatgc
    11041 ttttgtttaa atttatgttt tgcacaatac cgatactgat tgctatttac aggtttaagt
    11101 aaatcttctt tactccacgg agaagctgct ttggcgatca aaaaactacg tgaaaccgga
    11161 taatcagccc actcatgcca gggttttgta ggtcccacat agtgtataaa cacggtgtca
    11221 tcattgactg gattatcaac tttgtctttt aattcatagt taatactata tcgggtatta
    11281 tatttctccg aaataaactt cactttacca ttcaataata cattcagtac atcttgatca
    11341 aggtgggtga ttttacttac ccaatctggg tcacgcaaca tttcaatagc ttttgacgaa
    11401 atgttattta gattccactc atcaatattt atcagtaaaa aaccagcatt gaaatatcca
    11461 gaagctaact gtggtgtagt taataccgag gctcgatttt gccaccattc aatatctctt
    11521 tcagctacaa ctgcggcaat ttcattagta gaaaattgat aatctaagag ttctttaata
    11581 ctacccttgc aagcaatatc tgcatcaaga tatagtattt tttcatgttt atgatagaaa
    11641 taatctgcaa ttataaatcg aaaatatgtt gcgtaagtcc agtttttcgt gcttggtaat
    11701 gacttcagct tatcacaatt gataacataa atattaattc ttgaattata ttgttttgct
    11761 aaatcagaaa aatataattt gtctttatca ctgatataat ctgtgaaaac atgaaattca
    11821 caagagattt ctctattgtt taatagaata gatgcgatgg ctacaccaca tccaaaaaga
    11881 aaatttctgt caataccaaa tgctatatca agagtatttt tagatcttat attttgataa
    11941 tgaaaattat actcaaaaag gatgatgtca ctatcattga gttgagacat atttattctg
    12001 tatccaaaat tttggcaaaa ataagttacg ggttacaacg attttcgaat cgttctttcc
    12061 ctgatttttg tggcttttgc ttctgcttgc gacagcagtc cttgttcctg cttgagaatg
    12121 tctttaagcg gggcggcaaa atacaccttc ataaagcgcc agatatcccg ctgagtcagg
    12181 ccaatattca ttgaagaaaa ataaagccct ataagatctt tatcgcgcca acgacctgga
    12241 acgcgcgtgc gaagctgcgc ccggtgtagg tcaattaccg aaatttttaa ctcctcttcc
    12301 ttaccggaaa aaggcaagtg cagcaggaaa tgacagatat aacagtcacg atggttaatg
    12361 cccgcagcat gcatatcgcg caccatcgtc gcgacacgct taataagcat acgctttacg
    12421 cgaacatctg gtgggttggt cgcccagtca gcacagtaat cttccagact gatggttggt
    12481 gtcagatctt cggtaataat aaacgaggtt ctggtcagcg gattcatgcc tttttcgccg
    12541 aatgccaccc catacatagt atcaacgccg acatcccgca gtcgatgaat cgcattccat
    12601 tcgcggtctg cgcctaatac tggcatccgc aatgagagta aatttttgat tatctctttc
    12661 agggtcgtgc cgcgatgcca tttgagaaaa tagctttttc ccgccatttc aaagcgcaga
    12721 gtacggcgag tttccagttc acgaaatacc tcaccctgca aggttttaac ttcctcaaaa
    12781 ggatctttgc cgcgccataa cgtggcaaac ggctctttaa gttcaaccat ccagaccacc
    12841 cgttatgata tccgccgctt tctctggcag actgtataaa tcttgtgtat cagcataatg
    12901 tcgcgcattt tccgcccaag cctggcgcaa tgaagattgc gttaacgctt tgcgtaaaat
    12961 ctcattcaat gtttcctggc ggaatggctc agcaatagcc tcgccgcaat tagcgtcgac
    13021 aatataatgc gcatagccac aaacggcagt tgttagtacc ggtaatcctg cagtaatcgc
    13081 ttccagcagc acaattcccg ccgcttcctg gtaggcagga tgcagtaata aatccgccgc
    13141 cgccattaat tccgagacat cgttgcgccc cgagaagaag tgaacattac tgcgcacgcc
    13201 gcgtttttct gccagtgcct caaattttcg cggtttatcc tgcccaacaa catatagcaa
    13261 tgtgttgtgg cgcagcgaat ccggtaacga agcaagtgct tcaatggaac gatcggcacc
    13321 tttacgcgtg aagtctgaac cgacctgcaa caataaatat tgttgttcgg ttattccatt
    13381 cttcttacgg aagatttcac ggctattggc tggctgctgg ctatatttac gatcaggata
    13441 aatccccggt ggcagaatat gaaaacgctc cgcttcagtc tgataatgtt tctggaaatc
    13501 ggcgatttgc ttatctgtca gcatcagcag ctgtgtcggc ttgccctgtt cgaaggttgc
    13561 ccgctcaaag gcggcataat ggcgataacg tgacgtcagg cgatagaaaa agcctttttc
    13621 ctgcgctact ttctcggcat aacaaacatc agcggcataa taaacgtcca gccccggcat
    13681 tttgttgaat ccaacgactt tatcgacggg atgttcgcgt aaatgttttt gcacccaggc
    13741 aaaatactcc gcattgcgcc cgtgattggt atgcgattta accggcactt tgatcacttc
    13801 aaatacatca gggcattcgc cttcccacga ctgggtataa acccgaacat gatgacctcg
    13861 ggcggcgact gtctgagcaa tacgcataaa atcgcgctgc aaaccgccaa agggaaaata
    13921 tttatataaa caaaaagcaa cgatcataac gacgtacctg tcgtggagga gggcagtaat
    13981 ttatcgacag cagcaatgac atctgccgcc ggaataaccg aaagatacat ctcatttcgg
    14041 tcacgctgat cgcgcgttgg catttcccgg taatctcccg cccagaattg aatcatgtta
    14101 tttgaccagg gacgccagaa aatatgatct gttgcgccaa acagcgatat cagcggcgta
    14161 ttaactgcag cggcaatatg cgccggtgcg gaatcaacgc caataaacag ctgcgcatga
    14221 tcgattaacg caccaagttc cgggaaggtc acctttccag ccagcgccgt tactggtggc
    14281 gtctggcatc cctgcgcaat ttcattgacg caggccagat cgtctttatc tgggccggac
    14341 gtcagaacga cttcataacc acgagcatgt aaggcatcaa tcacagcgga aaacttggcg
    14401 ttgtcccagc atttgaagat ttgccgcgcc gtaggttgga taaccacata attttgtcca
    14461 acaccagcgt gatcaagttc gcgacgcata cgtttccagc ttgcaggcgg gtaactcatg
    14521 gttgtctgct tcaccaacga atcaagtccc aatggtgtca gcacggataa gttactttcc
    14581 accacatttc caccctgcaa cggcaccaaa tgggtgaaac ttttacgcca aaaagcagac
    14641 tgccgatgat gataatcctg ggaaattttc acacgagcat ttaataagcg aaccagtata
    14701 gcaaccatcc attgatcggt aagattgacg ataaggtcat acttattggc acgtaatacc
    14761 ttgatgagat gaaaaaagtt ggcaattttt tctgaggctt ttgctttttt attttttatg
    14821 ccgtagagcg cgttaatctc tggattttca gacaggatcg ggatggtgtc ctgataaagc
    14881 agcacatcga tttttgcgtc agggtaattt tttttcagag aactaatgac gggagtagtt
    14941 aataacatat ccccatgaaa acgcatctta atgagcaaaa ttcttcgaaa tggcttat
//
LOCUS       DAJVPT010000094        14971 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVPT010000094 DAJVPT010000000
VERSION     DAJVPT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(525..1466)
                     /gene="fabY"
                     /locus_tag="OQM47_002993"
                     /gene_synonym="yiiD"
     CDS             complement(525..1466)
                     /gene="fabY"
                     /locus_tag="OQM47_002993"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1481065.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1511..1948)
                     /gene="dtd"
                     /locus_tag="OQM47_002994"
     CDS             complement(1511..1948)
                     /gene="dtd"
                     /locus_tag="OQM47_002994"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1481066.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1945..2817)
                     /locus_tag="OQM47_002995"
     CDS             complement(1945..2817)
                     /locus_tag="OQM47_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1481067.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2811..3410)
                     /gene="yihX"
                     /locus_tag="OQM47_002996"
     CDS             complement(2811..3410)
                     /gene="yihX"
                     /locus_tag="OQM47_002996"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1481068.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3509..4294)
                     /locus_tag="OQM47_002997"
     CDS             complement(3509..4294)
                     /locus_tag="OQM47_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1481069.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4328..5224)
                     /gene="yihV"
                     /locus_tag="OQM47_002998"
     CDS             complement(4328..5224)
                     /gene="yihV"
                     /locus_tag="OQM47_002998"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1481070.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5392..6288
                     /gene="yihU"
                     /locus_tag="OQM47_002999"
     CDS             5392..6288
                     /gene="yihU"
                     /locus_tag="OQM47_002999"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1481071.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6312..7190
                     /gene="yihT"
                     /locus_tag="OQM47_003000"
     CDS             6312..7190
                     /gene="yihT"
                     /locus_tag="OQM47_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1481072.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7207..8448
                     /gene="yihS"
                     /locus_tag="OQM47_003001"
     CDS             7207..8448
                     /gene="yihS"
                     /locus_tag="OQM47_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1481073.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8586..9488
                     /locus_tag="OQM47_003002"
     CDS             8586..9488
                     /locus_tag="OQM47_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCS1481074.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9687..11723
                     /gene="yihQ"
                     /locus_tag="OQM47_003003"
     CDS             9687..11723
                     /gene="yihQ"
                     /locus_tag="OQM47_003003"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCS1481075.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11769..13143
                     /locus_tag="OQM47_003004"
                     /pseudo
     CDS             11769..13143
                     /locus_tag="OQM47_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13186..14589
                     /locus_tag="OQM47_003005"
     CDS             13186..14589
                     /locus_tag="OQM47_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1481076.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14657..>14971
                     /gene="ompL"
                     /locus_tag="OQM47_003006"
     CDS             14657..>14971
                     /gene="ompL"
                     /locus_tag="OQM47_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1481077.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGA"
BASE COUNT         3676 a         3854 c         3731 g         3710 t
ORIGIN      
        1 aaaattgatt taatagcgtt tgcttcggtg atttattact cattagttgt ttttctaaat
       61 taacaacaga ataactttct tgtttaatga gattaatatt ggcaaccatt atagcctcct
      121 tcccacggtt taagagcaaa catacaacat aatgattcat aacatacaga gcagtaaatg
      181 aatgatactg acaatgtatg ccagcccatt atctttgcat aatatttgtg agctatagat
      241 tttaaaaata tttatgaaaa aagtatggca ccagaggggg ttttgactat caaaaatcgc
      301 tcaaacctga actaatatga tgagttaagc attatctgca ataattgcca ctcagctcta
      361 caaataatat ttaaatgctc ctgaacagtg atgtagcaag acacgtatgg tgccaacgaa
      421 gaataaaaat ttataaagtt cttattcaat ataaccggaa tagtcaggga ctgatgtaaa
      481 tgaccggaat gaatgagaga ggcagcaccg cccctctcca taagctactc ttcttcgttc
      541 ccgccctctt catacgggcc aaatggcttc gcgggcagaa cgatatacgt gccttcaaac
      601 actgcacccg gcgtctcgtc gccaaagatt tcgacctgca tctgcacccg tgcttttcgt
      661 ccgcgcgcca gacggtcgag atcgccgctt aaggcaccaa ggtcggctac cgcatggggt
      721 ttaccgctaa tcggtttgct gtagcggata tgcgcatccg ccagaataat cgttccgccg
      781 aggtggcgtt cacgcagcat cagccagata agcccccaac cggtgagcgt cgccagcgag
      841 aataaactcc cggcaaacag cgtatggtgc ggattctgat tgccggtttc cggcatggtg
      901 gtgataaatt tttgcccggt atattgttga atacgcacgc ccattttttc actaagcggg
      961 atatgttcgt accacgcctg ttgcagctgc gcgcaccagt cgccgcgatg caaaatatca
     1021 tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa tcggcgtggt ggttggcgtg
     1081 gtgatttctc cctgattaac aaaccccagc ttggcgaaaa actccaccgc gtcttcacgg
     1141 gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca ccgactccag ggtcatcgcc
     1201 atcagcgttc ctaacccttt gtcctgcacg tccgggtgaa cgaccataaa gcgaatggac
     1261 gcttcattgt cggcattaat atacaatcgg cctaccgcca ccagattacc ctgctcgtcg
     1321 acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc gttccgaacc ttttggttga
     1381 tgcaggggct tacgcaacat ttcccagcga aagtgatagt aacgctctaa ctcttcttct
     1441 gtttgtggaa cctgaaggtg atacatagcg atactctctc ttgtttcccg tgacaaccct
     1501 ggaagctggc tcatacctgc aaccagaatg tcacggggcc atcattgacc agcgatacct
     1561 gcatatcggc agcgaagcgt cctgtttgcg tgttcatctc ttgctggcgg cagcgttcga
     1621 cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc gcctttggag aaacttgggc
     1681 gcatcccccg ttcggtatct gcggcgaggg taaactggga aaccaccagt acactgccgc
     1741 ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc gctaaagatg cggtagccga
     1801 gcacacgctc gcacagacgg tttgctttct gttcgtcgtc atccttttcg acacccaata
     1861 acaccaaaag tcccgcgcca atttcgcccg tcacttctcc ctccacggtg acgctggcac
     1921 gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct tcttcttgtt cagctgcttg
     1981 ttttagtttg cggtattccc cgagagtgac agtaatttcc gcgccaagca agacgataca
     2041 ccatgtccag tagacccaaa caaagagaat ggggatcacc gccagcacac cgtaaatgag
     2101 ctgatatgac gggaacatgg tgatataaag cgcgaaacct ttctttcctg cttcgaacag
     2161 gagtgcggcg acaaacgcgc cgacaatcgc gtcgcggtta ggtacgcgga tggtaggaac
     2221 aatgctgtac agcaaccaga aggagatcca cgacaacagc aacggaaaaa tacgcagcac
     2281 gttgtcgatg acagtattga gatcgctcgc ccagcgcagg gagagcaaat aggaactgat
     2341 cgccagactg gcccctgcca gcagcggccc cagcgttaaa atcatccagt acacggcgaa
     2401 cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg gtattcaacg cgctatcgat
     2461 ggagtacatc aacaataacg cggtgacgat cagcccgcac gccccaacgg cggtcatctt
     2521 gttggaatta gcaacaaatt gttcgatata ccgctgaata acatcgccag tagcaggcag
     2581 aaagttggca aaaataaagt gacgtaactg aatgctgacg tcggaaaaca tgggaaaagc
     2641 ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat gagagcaacg acacataggc
     2701 aaggttacct gccagggttg tcatgttgtc ctcatcaata cgttgccaga gtagttttag
     2761 ccaggcccat agtggacggg tacggtgcct ggctttgtcc tgaatggttt ttagcataac
     2821 accttcgcga aatagtccgg gatggtggtt ttatctttca ccagaatact ggtaatgcct
     2881 agctgattag ctccttctat attatcggcg ttatcgtcga aaaagaccgt atcgctgggt
     2941 gaaaaacctt ccgcctgcaa aacatgctgg taaattcgtg cttcaggttt gcgcatcccc
     3001 agatcttgcg acagatagat atggtcagca gcatcacgaa tttccgggta ttcttccggc
     3061 cagaaggtgg tatgcaggcg gttggtattg gaaagcacca ccacgcgatg cccctgctca
     3121 cgcagtttat gcatgatggc gatcacttcc gggcgcagcg caacaaacac cgcctgccag
     3181 ccgtgagaga actgctcgta gcttagcggt agagccatct catgacacag cgcctctgcg
     3241 aacgcttcgt cgctaatttc cccacgctca tgctgatgaa acgcctcccc catatgaaaa
     3301 ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc tccaggctcc cagcacacgg
     3361 ttaaagtcga tatcgacaat cacattacct aaatcaaaga tatagagcat ttttgcctcc
     3421 tttcgcgcca caagaaatta actgtagcgg gaaaggatgg gtttggctat gtggcaaggt
     3481 atgaagccag attaacggtc acaaaaatct acgcgtcttc ctggggaaga atgatttcaa
     3541 tttggctgtc ttgtaagcgt gattttagcg cagcgggcgg cagctcgtcg gtaaagagcg
     3601 cagtgacctg tgccacgtta ccaatttcaa ccgccgccga agcatgatac ttagtgtgat
     3661 cggcgaccag cagaatattt ctcgcgtgcg ccatcatcgt tttcaccacg ttagcttcgt
     3721 ttacatcaaa ctccatcaac gcgccatcgc tctcaatcgc cccaacgctt gttaccagat
     3781 aatcagcacg aaaatcagcc acaaaggacg ccgctgaagg gccaatgatc ccgctattat
     3841 gagagcgcaa cgtaccgccg ggcaccatca cttcaaagcg cgggttgtgg taaagaatat
     3901 gcgccacacg caggctgttg gtgattatcc gcaaatgatt atggttaagt aacgcccggg
     3961 caacatgctc aacagtcgta ccaatggtga taaatattgt tgaaccatca ggaatatagt
     4021 ctgccaccgc ttcggcaatc gctttttttt tctcggtttg cgaaacctca cgctgctcga
     4081 acgccgtatt aacgacgctg gaagcccgac ccgcgccacc gtgatggcgc gtaatcaggc
     4141 cttgctcgct taatttacga atatcccggc ggaccgtctg cgtggagaca tccagcagat
     4201 ttgccagctc atcaatattc atatacccac gctcggcgat cagcatgagg agttggtcgt
     4261 gccgcgggtt accggttagt tcggtaaggc tcatgaaaat tcctcgaaaa accatcaccc
     4321 tggcatttta tacaaaaagt gacaaaaaag atcgggtttg atcacagtca gggatcccgg
     4381 ctcgtccacc gggacgtgtg cattttaacg ccgctacacc gctggcgaag cggactgact
     4441 ccgctaaatc cccacttgtt gccagcgcca ccgccaaagc gccgtgaaaa acatctcccg
     4501 cacctgtggt atctaccaca tcaactttga aggccggttg atgctgacgc ccaccatttt
     4561 ccagccagtc gcagcctgcg ctaccctggg tcacatagac atgaccattt gtgagcgttt
     4621 gtgcctgttt tagcgcactg gccatctctt tcacgcccgt taagcgcgcc agacccggtt
     4681 ctgaaaaggc cgcgtgatcg cttaatgcca ccagctcact gatatcctgc ggcgtaatgt
     4741 ccccgtccag aacggtcatc acacccgcct gacgggccag ggtgaaggct tttttagcgc
     4801 cgtcgtgcca gcgtacatct gccagcacaa catcccactg agagaaatca atttcctcca
     4861 accactctgc gtcaggcagc agatccgggc tggggtagtt aatgattatc cgctcgcctt
     4921 tggtatccac catgatggcg gattgcgaag atttcgcctg gttataccgt ttggtgtaac
     4981 gggtgttaac gccccaggat tccagctctg ccagcaggct gttgcccgcg tcgtcatcac
     5041 ctacgcgacc aataaaatcg acctgcgccc ccagccttgc cgccgcaacc gctgccgtcg
     5101 ccgctggccc gccaccaact tccgtataat ttctcgccac gtatttaccg ctctccgtcg
     5161 gtaacccttc cacgtaatag atgcgatcca tcacggttat acctacacaa gcaacacgaa
     5221 tcatggtcat tccttaaaca ttttgggatg cgttcatttt aatttcacca attgtttaaa
     5281 aagtgacggc tgtcaatttt ttgacgataa attacaaatt cgatcaaaaa cagacaagca
     5341 aagcatgtca taaatgacaa aaagtgacat cactgtattc aggagagggt tatggcagca
     5401 atcgcgttta tcggtttagg acaaatgggt tcgccaatgg cgagcaattt attgcagcaa
     5461 gggcatcaac ttcgcgtctt tgatgtgaac gccgaagctg tgcggcatct ggtagacaaa
     5521 ggtgcgactc ccgccgccaa cccggcgcag gccgctaaag atgccgaatt tatcattacc
     5581 atgttgccga atggcgatct ggtgcgcaac gtgttgttcg gtgaaaacgg cgtttgcgaa
     5641 ggcttatcta ccgatgcgct ggtcattgat atgtccacca tccatccgct gcaaaccgat
     5701 aaattgattg ccgatatgca agccaaaggc ttcagcatga tggatgttcc ggtaggccgt
     5761 acttctgcaa atgccattac cggtactctg ttactgctgg ctggcggcac cgctgaacaa
     5821 gttgaacgtg ccacaccgat cctgatggcg atgggcagtg agttgatcaa cgctggcggt
     5881 ccgggcatgg ggatccgcgt taagctcatc aacaactaca tgagcatcgc gctcaatgcg
     5941 ctttcggcag aagccgccgt tttgtgcgaa gccctgaatc ttcccttcga tgttgccgtc
     6001 aaagtgatga gcggtaccgc cgccggtaaa ggtcacttca ccacttcctg gccaaacaaa
     6061 gtcctcagcg gagatctttc tcccgccttc atgatcgatc ttgcccataa ggatcttggc
     6121 atcgcccttg atgtcgccaa ccagctgcat gtgccaatgc cgctgggggc cgcctcacgg
     6181 gaggtttata gccaggcgcg cgcagcgggt cgcggtcgcc aggactggtc cgccattctg
     6241 gaacaggtcc gtgtcagtgc cgggatgact gccaaagtaa aaatgtaacg actggataaa
     6301 ggaataaatg aatgaataag tacaccatca acgacattac gcgcgcatcg ggcggttttg
     6361 ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat gtttgccgcg gctggggcac
     6421 ccgctccggt agccgatagc gttttaactg atttcaaagt taacgctgca aaggccctct
     6481 cgccttatgc ctcggcgatt ctggtagatc aacaattctg ctaccgccag gtggttgagc
     6541 aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc cgatgagttc attcctggca
     6601 acggtattcc ggtcgatagc gtggttattg accgcaaaat caatccgcta cagatcaaac
     6661 aagacggtgg caaagcctta aaactgctgg tgctgtggcg tagtgatgaa gatgcgcagc
     6721 aacgtctgga tatggtgaaa gagttcaacg aactgtgcca ctcacacggt ctggtaagca
     6781 tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa attcgatcgc gaacaagcga
     6841 tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga cctctacaaa gttgaaatgc
     6901 cgctttatgg caaaggtccg caacaagagc ttctctgtgc ttcacaacgt ctgaatgacc
     6961 atatcaatat gccatgggtg atcctctctt ccagtgtcga cgaaaaactg ttcccgcgtg
     7021 ccgtacgcgt ggcaatgacg gcgggcgcat cgggattcct ggcaggccgt gcagtctggg
     7081 catcggtcgt cggtttacca gacaacgagc tgatgctccg tgacgtttgc gcaccgaaat
     7141 tacaacaact tggcgatatc gtcgacgaaa tgatggctaa acgccgctaa gatgaggata
     7201 aaaggaatga aatggtttaa caccctaagc cacaaccgtt ggctggaaca ggaaaccgac
     7261 cgcatctttg attttggtaa aaattccgta gtgccgactg gttttggctg gttaggcaat
     7321 aaagggcaaa tcaaagaaga gatgggcacc catctgtgga tcacagctcg tatgttgcac
     7381 gtttattccg ttgctgcggc gatgggtcga cctggcgctt acgcgttggt tgatcacggt
     7441 attaaagcca tgaacggcgc actgcgtgat aaaaaatatg gcggctggta tgcctgcgtg
     7501 aatgacgagg gcgtggtgga tgcctccaaa cagggctatc aacacttctt tgctctgctg
     7561 ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc gcaagctgct cgattacacc
     7621 attgagatta tcgagaaata tttctggagc gaagaagagc agatgtgcct ggagtcctgg
     7681 gacgaagcct tcagcaaaac cgaagagtac cgcggcggca atgccaatat gcacgcggtg
     7741 gaagctttct tgattgttta tgacgtcact cacgacaaaa aatggctgga tcgcgcgatt
     7801 cgcgtggctt ccgtgattat ccacgacgtc gccagaaata atcattatcg cgttaacgaa
     7861 cacttcgata cccagtggaa tccgctgccg gattacaaca aagataaccc ggcgcatcgc
     7921 ttccgcgcgt tcggaggtac accaggccac tggatcgaat ggggccgttt aatgctgcac
     7981 atccatgctg ccctggaagc ccgttgcgaa caaccaccag catggctgct agaagatgcc
     8041 aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac ccgatggagc ggacgggatt
     8101 gtttataccg ttgactggga aggaaaaccg gtggtccgcg aacgtgtacg ttggcctatc
     8161 gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca ctggcgatcg ccagtatgaa
     8221 acctggtatc aaacatggtg ggagtactgc attaagtacc tgatggacta tgaaaatggt
     8281 tcctggtggc aggagctgga tgcggacaat aaggtcatca ccaaagtctg ggacggcaaa
     8341 caggatattt atcacctgct gcattgcctg gtgatcccgc gtatcccgtt agcgcccggc
     8401 atggctccag cggttgcagc gggtctgctg gatattaatg cgaaataact gatctgaaaa
     8461 tagggtgcgg gttttcccct caccctaacc ctctccccaa aggggcgagg ggaccgtccg
     8521 gtgcagtttt tgggtttgac atcagcctca cgggaggctg tatgtcgtta attaaggtgc
     8581 cttgtatgca aatcaccaat atgcattgca gtggacagac cgtttcactt gccgcggggg
     8641 attatcacgc gaccattgtg accgtcggtg ctggtctggc tgaattaacc tttcagggtt
     8701 gtcatttagt catcccgcac aaacccgaag agatgccgct ggcgcatctg ggtaaagtgc
     8761 ttattccgtg gccgaaccgg atcgccaacg gctgttaccg ttatcagggg caggaatatc
     8821 agttacccat taatgaacat agttcaaaag ccgctattca cgggctgctt gcctggcgag
     8881 actggcaaat tagtgaatta accaccacca gcgttaccct gacagctttt ctgccgccca
     8941 gttacggcta tccgtttatg ctggcttcac aagttgttta ttcgctcaac gcacacacgg
     9001 gtctttccgt tgagatcgca agccagaata tcggcactgt cgcagcgcct tatggcgtgg
     9061 gtattcatcc ttatttaacc tgcaacttaa catctgttga tgaatatctg ttccagcttc
     9121 cggcaaacca ggtttatgcc gtcgatgaac acgtaaatcc gacaacgctt caccacgttg
     9181 atgagctgga tttaaatttc acgcaggcga aaaaaatagc cgcgacaaaa atcgaccaca
     9241 cctttaaaac cgctaatgat ttgtgggaaa tgaccattac ccatccacag caggcgcttt
     9301 cggtaagcct ttgttccgat caaccctggg tgcaggttta tagcggtgaa aaattacagc
     9361 gtcagggact ggcagttgaa cctatgagtt gcccgcctaa cgcctttaat tcaggtatcg
     9421 acttattatt actggaacca ggaaaaccgc accgcctgtt ttttaatatt tacggacaga
     9481 gaaaataact tgtattgatt agcagtcaaa atcattgaag aacatttcac tcgctaaagg
     9541 caacattatg tatcttcaat ttattaagtt aaaaacagct cagcttctgt aaagttttta
     9601 tatatcagtt ttatgagaat cattttacat aagcctgtaa taacaggaag gcaacaatct
     9661 cgttctcaac tctatggaga ttagttatgg atacgccacg tccacagtta ttagattttc
     9721 aatttcatca gaataacgac agttttaccc tacattttca acaacgtctt attttaaccc
     9781 ataacaaaga taatccttgt ttatggattg gctcaggtat agcggatatc gatatgttcc
     9841 gcggtaattt cagcattaaa gataaactac aggagaaaat tgcgcttacc gacgccatcg
     9901 tcagccagtc accggatggt tggttaattc atttcagccg tggttctgac attagcgcca
     9961 cgctgaatat ctctgccgac gatcagggcc gtttattgct ggaactacaa aacgacaacc
    10021 ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat atctacggct
    10081 gcggcgaaca gttttcctac ttcgatctgc gcggcaaacc gttcccgcta tggaccagtg
    10141 aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg gcaggccgac tgcaaagaaa
    10201 atgcgggcgg cgactattac tggactttct tcccacagcc tacgtttgtc agcacgcaga
    10261 agtattactg ccatgttgat aacagttgct acatgaactt cgactttagc gccccggaat
    10321 accatgaact ggcgctgtgg gaagacaaag ctacgctacg ttttgaatgt gctgacacat
    10381 acatctccct gctggaaaaa ttaactgccc tgctgggacg ccagccagaa ctgcccgact
    10441 ggatttatga cggggtaacg ctcggcattc agggcgggac ggaagtgtgc cagaagaaac
    10501 tggacaccat gcgtaacgcg ggcgtgaagg ttaacggcat ctgggcgcag gactggtctg
    10561 ggatccgtat aacctctttt ggcaagcgcg tgatgtggaa ctggaagtgg aacagcgaaa
    10621 actacccgca actggattca cgcattaagc agtggtatca ggagggcgtg cagttcctgg
    10681 cctatatcaa cccgtatgtt gccagcgata aagatctctg cgaagaagcg gcacaacacg
    10741 gctatctagc aaaagatgcc tctggcggtg actatctggt ggagtttggc gagttttacg
    10801 gcggcgttgt cgatctcact aatccagaag cctacgcctg gttcaaggaa gtgatcaaaa
    10861 agaacatgat tgaactcggc tgcggcggct ggatggctga cttcggcgag tatctgccca
    10921 ccgacacgta cctgcataac ggcgtcagtg ccgaaattat gcataacgcc tggcctgcgc
    10981 tgtgggcgaa gtgtaactac gaagcccttg aagaaacggg caagctcggc gagatcctct
    11041 tctttatgcg cgccggttct accggtagcc agaaatactc caccatgatg tgggcgggcg
    11101 accagaacgt ggactggagt ctcgacgatg gcctggcgtc cgtagtcccg gcggcgctgt
    11161 cgctggcaat gaccggacat ggtctgcatc acagcgacat tggcggttac accaccctgt
    11221 ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc gccttcacgc
    11281 cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt gacggcgatg
    11341 cggaaactat cgcccatttt gcccgtatga ctaccgtttt caccaccctg aaaccttatc
    11401 tgaaagaggc cgtcgcgctg aatgcgaagt ccggtctgcc ggtcatgcgc ccgctgttcc
    11461 tgcattacga agacgatgca catacctatt ccctgaaata tcagtacctg ttaggccgcg
    11521 acattctggt cgctccggta cacgaagaag gtcgcagcga ctggacgctc tatctgccgg
    11581 aggataactg ggtacatgcc tggacgggtg aagcgttccg gggcggggaa gttaccgtta
    11641 atgcgcccat cggcaagccg ccggtctttt atcgcgccga tagcgaatgg gcggcactgt
    11701 tcgcgtcgtt aaacagcatc taatcacatc cgcccgtggg gaaactcatg ggcaaaggga
    11761 gaacatcgat gagtcacatc acaacggaag atccagcaac tttacgcctg ccctttaaag
    11821 agaaactctc ttacggtatt ggcgacctgg cctctaacat cctgctggat atcggtacgc
    11881 tttatctttt gaagttttat accgacgttc tggggctgcc aggcacctat ggcggcatta
    11941 tctttttgat ttcaaaattc tttactgcgt ttaccgatat gggtaccggc attatgttgg
    12001 attcccgacg caagatcggt ccaaaaggta agttccgtcc ttttattctg tatgcgtcat
    12061 tcccggtcac cttactggcg atcgccaact ttgtcggcac accgtttgat gtcaccggta
    12121 aaacggtgat ggccactatt ctgtttatgc tttacggact gtttttcagc atgatgaact
    12181 gctcctacgg cgcaatggtc ccggcaatca ccaaaaaccc caacgaacgc gcctcactgg
    12241 cggcatggcg tcagggcggc gctacgctgg gcctgctgct gtgcacggtg ggattcgtgc
    12301 cagttatgaa tcttatcgaa ggtaatcaga aacttggcta tatcttcgcc gccacgctgt
    12361 tttcactgtt cggcctgctg tttatgtgga tctgctactc aggcgtgaaa gagcgttatg
    12421 tcgaaaccca accaaccaat ccggcgcaaa agcctggcct gttgcaatct ttccgcgcaa
    12481 ttgccggtaa ccgcccactg ttcattctgt gtattgccaa cctctgcacc ttaggggcgt
    12541 ttaacgtcaa gctcgccatt caggtctatt acacccagta cgtacttaac gatcccatcc
    12601 tgttgtcgta tatgggattt ttcagcatgg gctgtatttt catcggcgtg ttcctgatgc
    12661 ctggcgcagt cagacgtttt ggtaagaaga aggtctatat cggcggcctg ctgatttggg
    12721 tgctgggcga tctgctcaac tatttcttcg gcggcggctc ggtcagcttt gtggcgttct
    12781 cctgcctggc gttcttcggc tcagcgcctg aactgggcgc tggtttccga caccgtcgag
    12841 tacggcgagt ggcgcaccgg cgtgcgttcg gaaggcacgg tctacaccgg cttcaccttc
    12901 tttcgcaaag tgtctcaggc gctggctggt ttcttccccg gctggatgct gacgcaaatt
    12961 ggctatgtgc cgaacgtcgc acaggctgac cacaccattg aagggttacg ccagttgatc
    13021 ttcatctacc caagcgcact ggcggtagtc accattgtgg cgatgggttg cttctacagc
    13081 ctgaacgaga agatgtatgt ccgcattgtg gaagagatag aagcccgtaa acgcacggcg
    13141 taataataat aaacgacgcc ctgcggggcg ttataaggag tgattatgtc tgaccataat
    13201 ccactgacat taaaactgaa tctgcgggaa aaaatcgcct atggtatggg cgacgtcggt
    13261 tcgaatttaa tgctctgcat cggtactctg tatctcctca aactttatac cgatgaatta
    13321 gggatgcctg cttactatgg cgggattatc tttctggtcg cgaagttttt taccgcgttt
    13381 accgatatgc tcaccggatt tttactcgac tcgcggaaaa atattgggcc taaaggcaaa
    13441 tttcgtccct ttattttata tgcagcagtt ccggcggcgt taattgcaac gctccagttt
    13501 atcgccacca ccttttgttt gccggttaaa acgacgattg ccaccacgct gtttatgatg
    13561 tttggacttt catacagtct gatgaactgc tcgtatggtg cgatgatccc ggcaattacc
    13621 aaaaacccga atgagcgcgc gcagctcgcg gcttaccgcc agggtggtgc aaccataggg
    13681 ctattgattt gtaccgtggc gtttattccg ttgcagtcgc ttttttctga ctcaaccgtc
    13741 ggttatgcct gtgcggcact tatgttctcc attggcggct ttatttttat gatgctgtgc
    13801 tacagaggcg tcaaagagca ttatgtggac acagcgccaa ccggacataa agccagtatt
    13861 ctcaaatctt tttgcgcgat atttcgtaat ccaccattgc tggttttatg cattgctaac
    13921 ctgtgtactc tggcggcatt taatatcaaa ctggcgattc aggtctatta cacccagtat
    13981 gtgctgaatg atattaattt attgtcgtgg atgggattct tcagtatggg atgcatcctc
    14041 gtcggcgtat tactggtgcc agtaactgta aaatgttttg gtaaaaaaca ggtttattta
    14101 gctggcatgg tgctatgggc ggtgggtgat atactgaatt atttctgggg aagtaactct
    14161 ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca cggcctttgt aaacagcctg
    14221 aactgggcac tggtaccaga taccgtagat tacggtgaat ggaaaaccgg tattcgtgcc
    14281 gaaggttctg tttataccgg ttataccttc tttcgtaaaa tttctgccgc acttgctggc
    14341 ttcttgccag gcattatgct gacgcaaatt ggttatgttc ccaacatcgc gcaaagcgat
    14401 gcgacattac agggtttgcg tcagctcatt tttatctggc cttgtgcact ggcaattatt
    14461 gctgcattga cgatgggatt cttttacaca ctcaatgaaa aacggtttgc attgattatt
    14521 gaggaaatca accaacgcaa aaataaagag atggcgacag aaaaaaaaac ggcttccgta
    14581 acgttataaa tgtcaatagc cgctatttcc atcctggtgg gtggcggcct ccctacgttt
    14641 aaaaaatgga cttattatga aaaagattaa tgcaataatt ttattatctt ctctgacctc
    14701 tgcctcggta tttgctggcg catacgtcga aaacagggag gcatataatc ttgcctccga
    14761 tcagggcgag gttatgctac gtgtgggtta taacttcgat atgggcgcgg gtattatgtt
    14821 aaccaatacc tacacctttc agcgagaaga tgaactaaaa catggctata atgaaattga
    14881 aggttggtat cctttattca aaccaaccga taaattaacc atccagcccg gtggcttaat
    14941 taatgataag agtatcagtt cgggtggtgc a
//
LOCUS       DAJVPT010000095        14838 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVPT010000095 DAJVPT010000000
VERSION     DAJVPT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..651)
                     /locus_tag="OQM47_003007"
     CDS             complement(<1..651)
                     /locus_tag="OQM47_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1481078.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAG"
     gene            925..1392
                     /gene="slyB"
                     /locus_tag="OQM47_003008"
     CDS             925..1392
                     /gene="slyB"
                     /locus_tag="OQM47_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1481079.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1439..1873)
                     /gene="slyA"
                     /locus_tag="OQM47_003009"
     CDS             complement(1439..1873)
                     /gene="slyA"
                     /locus_tag="OQM47_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1481080.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2074..2310
                     /locus_tag="OQM47_003010"
     CDS             2074..2310
                     /locus_tag="OQM47_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1481081.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2313..3170
                     /locus_tag="OQM47_003011"
     CDS             2313..3170
                     /locus_tag="OQM47_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1481082.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3170..5182
                     /locus_tag="OQM47_003012"
                     /pseudo
     CDS             3170..5182
                     /locus_tag="OQM47_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5183..5704)
                     /gene="sodC"
                     /locus_tag="OQM47_003013"
     CDS             complement(5183..5704)
                     /gene="sodC"
                     /locus_tag="OQM47_003013"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1481083.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5785..6681)
                     /locus_tag="OQM47_003014"
     CDS             complement(5785..6681)
                     /locus_tag="OQM47_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1481084.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(6730..6969)
                     /locus_tag="OQM47_003015"
     CDS             complement(6730..6969)
                     /locus_tag="OQM47_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1481085.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7072..7671
                     /gene="nemR"
                     /locus_tag="OQM47_003016"
     CDS             7072..7671
                     /gene="nemR"
                     /locus_tag="OQM47_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1481086.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7708..8805
                     /gene="nemA"
                     /locus_tag="OQM47_003017"
     CDS             7708..8805
                     /gene="nemA"
                     /locus_tag="OQM47_003017"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1481087.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8886..9293
                     /gene="gloA"
                     /locus_tag="OQM47_003018"
     CDS             8886..9293
                     /gene="gloA"
                     /locus_tag="OQM47_003018"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1481088.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9396..10043
                     /gene="rnt"
                     /locus_tag="OQM47_003019"
     CDS             9396..10043
                     /gene="rnt"
                     /locus_tag="OQM47_003019"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1481089.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10136..12250
                     /locus_tag="OQM47_003020"
     CDS             10136..12250
                     /locus_tag="OQM47_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1481090.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12260..13552
                     /locus_tag="OQM47_003021"
     CDS             12260..13552
                     /locus_tag="OQM47_003021"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481091.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14502..14594
                     /locus_tag="OQM47_003022"
     CDS             14502..14594
                     /locus_tag="OQM47_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCS1481092.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(14638..>14838)
                     /locus_tag="OQM47_003023"
     CDS             complement(14638..>14838)
                     /locus_tag="OQM47_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1481093.1"
                     /translation="CGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLAC
                     GEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3379 a         3925 c         3903 g         3631 t
ORIGIN      
        1 acccgcccgt gcccactcgg catctttatc gtaaggttta cctgcctgac gccagatcca
       61 ggcatccatc agcatgttac caggaccggt atcgtagccg ccaaccagct gcccaggaat
      121 gagcagtgac agattggcga tgccgccaat attgagcacc attcgtcgct cggttgggtg
      181 agccagcaag gcatgatgga acgcaggtac cagcggtgcg ccttgtcctc ccaaggcaat
      241 atcgcgacgg cgaaaatcac cgacaaccgt aattccggtg cgtgccacaa tttgattgtt
      301 atcgccaatc tgtaaagtgt gtggtgccac gcccgtcggt tcatgccaga cggtttgacc
      361 gtgacagccg atcgcaacta tatctctcgc ctgcaggttt tgttccttaa gcaaggcatt
      421 gaccgcatca gcaaaaagtc gcccgagttg agtatcaagc tgtccaaact gcgaaagtgt
      481 aagctgctgg ccctggcaaa tatccagtac agcctgtttc agagacaccg ggatcggcca
      541 gctcaagctt gccagctgtg cgacccggtg ttcatcaatt gtcgccaaca caacatcaac
      601 accatcaagg ctggtgcctg acataacgcc aataaagcgg cccgatttca tagttcatcc
      661 tttttcaatc tgacgtttgc gcaccactca aacataaact tttcgtgaat accatgcgga
      721 atgaccgatt tttaccgttg gtagtaaaac attatcttca aatcaataat catcatgaat
      781 gttttgttta taattggttg atcctacttt cattatgatt tgctcatatt tggtagaaca
      841 tgtaaccatg gattcacata tgccatatac tttgaccatg agggatgctt gcgtggcgtt
      901 tcatggtgaa caggagattt ttcaatgatt aaacgcgtat tggttgtttc aatggtaggt
      961 ctgtctcttg tcggttgtgt taataacgac accctgtcag gggatgttta taccgcttct
     1021 gaagcgaaac aagtacagaa tgtcagctat ggcaccatcg ttaacgtacg tccggtacag
     1081 attcagggcg gtgatgattc caacgttatc ggtgcaattg gcggtgctgt tcttggtggt
     1141 ttcctgggga atactgttgg tggcggaacc gggcgttctc tggctactgc agcaggcgct
     1201 gttgcaggtg gcgtagctgg tcagggcgta cagagtgcaa tgaacaaaac gcagggtgtc
     1261 gagctggaaa ttcgtaaaga cgatggtaat accatcatgg tggtacagaa acaaggcaac
     1321 actcgtttct ctccgggcca acgtgtcgta ctggccagca atggcagtca ggtgaccgtt
     1381 tctccgcgct aaataagttt gcgtgtggtc aggttactga ccacacgccc ccttcatttc
     1441 accctttggc ctgtaactca atgatattat gctcaagttt tgcgatgagc gtaatcagtt
     1501 gctccagttc ctctgcggag atgccatgta atatttccgc gcgggttttg ttaataacag
     1561 cttccatttc gctgatcagt ggctctgcct tttccgtcag tttaatacgt ttagcccgac
     1621 gatcgctggc acaagtttga cgcgaaatta accctttttc ttccagttgg tccagagtac
     1681 ggaccagtga tggctgctcg atgccaatcg cttttgccag ttgaatttgc gactggtctg
     1741 gaggtaactg atggatattg tgtaacgtaa cccaatgggt ttgtgttaac tccagcggtt
     1801 tcaggcgatg gtctatcaga gcacgccata tgcgcaccaa ccgtgccaga tcagaaccta
     1861 gtggcgattc caatttcatc tccttataat tagcttgcta agatattatg cggcttttag
     1921 aatagtgtgc agcaattgta ttgctaaaac aaatgtattg ctgcatttgg ttaccgtcag
     1981 acatattttt cagaaattgc gcgtaaattt ttcgcactta aagaatattt attaatctaa
     2041 cgcaatatat tcggtcgtaa aggaatctac tttgtgaagt ttatgctcaa tgcaacagga
     2101 ttgcccttac aagatctggt gttcggtgcg tccgtctatt ttcctccgtt tttcaaagca
     2161 ttcgcgtttg gattcgtcat ctggcttgtc gtacaccgcc tgcttcgtgg ctggatctac
     2221 gccggcgata tctggcatcc cttgttaatg gatttatcgc tgtttgcgat ttgcgtttgc
     2281 cttgctctgg caatactgat tgcgtggtaa ctatgtcaat taaaacaatt aagtatttct
     2341 caacaatcat tgtagcgata gttgcggttc ttgccggatg gtggctgtgg aattattaca
     2401 tgcaatctcc gtggacacgc gacgggaaaa tacgcgcaga acaggtttct atcaccccac
     2461 aggtgtcggg acgtattgtt gagctgaata taaaagacaa ccaactggtt aacgcggggg
     2521 aacttctgct cacgatcgat aaaacgccct ttcagatcgc cgagctgaac gcacaggctc
     2581 agctggcgaa agcgcaatct gacctcgcaa aagccaataa cgaagcgaat cgtcgtcgtc
     2641 atctctcaca aaattttatt tttgccgaag aactcgatac cgctaacctc aatgttaaag
     2701 cgatgcaggc cagtgttaat gccgcacagg cgacgctgaa acagacacaa tggcaactgg
     2761 cgcaaacgga aattcgcgcc ccggtgagtg gatgggtgac taacctcact acccgcatcg
     2821 gcgattacgc tgacaccggg aaaccgctgt ttgcccttgt cgacagccac tcgttttacg
     2881 tcattggtta ttttgaagaa accaagttgc gccatatccg cgaaggtgca ccagcacaaa
     2941 ttaccctcta tagtgataac aaaacgttac agggtcacgt ttccagtatc ggtcgggcga
     3001 tttatgatca gagcgttgaa agtgactcaa gccttattcc ggatgtgaaa cctaacgtcc
     3061 cctgggttcg tctcgcccaa ctcgttcccg tccgctttgc gctggataaa gttccaggcg
     3121 atgtcacgct ggtgtcgggc accacctgta gcattgccgt aggtcaataa tgaacgcatc
     3181 gtcatggtcc ttgcgcaatt tgccctggtt cagggccacg ctggcgcaat ggcgttatgc
     3241 gttacgcaat accattgcca tgtgtctggc gctgacggtt gcctattatt tgaatctgga
     3301 tgaaccctat tgggcgatga cctcggctgc agtggttagc tttcccaccg ttggcggtgt
     3361 catcagcaaa agcctcggac gcatggctgg cagtttactg ggagccattg cggcactgct
     3421 tcttgccgga catacgctca atgagccgtg gttttttcta ttgagcatgt cggcgtggct
     3481 tggcttttgt acctaggcct gtgcgcactt cacgaataac gtcgcgtatg catttcaact
     3541 ggcgggctac acggctgcca tcatcgcctt tccgatggtt aatattactg aggccagcca
     3601 gctgtgggat atcgctcagg cgcgcgtttg cgaggtgata gtcggtattt tgtgcggcgg
     3661 catgatgatg atgatcctgc ctagcagttc cgatgctact gcccttttaa ctgcattgaa
     3721 aaacatgcac gcccgattac tggaacatgc cagtttactc tggcagcctg aaacaaacga
     3781 tgccattcgt gcagcacatg agggggtgat tgggcagata ctgaccatga atttgctgcg
     3841 tatccaggct ttctggagcc actatcgttt tcgccagcaa aacgcgcgcc ttaatgcgct
     3901 actccaccag caattacgta tgaccagtgt catctccagc ctgcgacgta tgttgctcaa
     3961 ctggccctca ccgccaggtg ccacgcgaga aattctcgaa caattgctga cggcgctcgc
     4021 cagttcgcaa acagatgctt acaccgtcgc acgtattatc gccccgctac gcccgaccaa
     4081 cgtcgccgac taccggcacg tcgccttctg gcagcgacta cgttattttt gccgccttta
     4141 tctgcaaagt agtcaggaat tacatcgtct gcaaagcggt gtagatgatc atgccagact
     4201 cgcacggaca tccgctctgg ctcgtcatac cgacaacgcc gaagctatgt ggagcgggct
     4261 gcgtacattt tgtacgttga tgatgattgg cgcatggagt attgcatcgc aatgggatgc
     4321 cggtgccaat gcattaacgc tggcagcaat tagctgcgta ctctactccg ccgtcgcagc
     4381 accgtttaag tcgttgtcac tgctgatgcg cacgctggtg ttactttcgc tattcagctt
     4441 tgtggtcaaa tttggcctga tggtccagat tagcgatctg tggcaatttt tactgtttct
     4501 ctttccactg ctggcgacaa tgcagcttct taaattgcag atgcccaaat ttgccgcact
     4561 gtgggggcaa ctgattgttt ttatgggttc gtttatcgct gtcactaatc ccccggtgta
     4621 tgattttgct gattttctta actataatct ggcaaaaatc gttggcgttg cgctggcttg
     4681 gctagcgttc gccattctgc gtccaggatc ggatgctcgt aaaagccgcc gccatattcg
     4741 cgcgctgcgc cgggattttg tcgatcagct aagccgccat ccaacactga gtgaaagcga
     4801 atttgaatcg ctcacttatc atcacgtcag tcagttgagt aacagccagg atgcgctggc
     4861 tcgccgttgg ttattacgct ggggtgtagt gctgctgaac tgttctcatg ttgtctggca
     4921 attgcgcgac tgggaatcgc gttccgatcc gttatcgcga gtacgggata actgtatttc
     4981 actgttgcgg ggagtgatga gtgagcgtgg cgttcagcaa aaatcactgg cggccacact
     5041 tgaagaatta cagcggattt gcgacagcct tgcccgtcat catcaacctg ccgcccgtga
     5101 gctggcggca attgtctggc ggctgtactg ctcgctttcg caacttgagc aagcaccacc
     5161 gcaaggtacg ctggcctcgt aattacttaa ttacaccaca ggcatagcgt tcaccgccac
     5221 cgcccagcgg tttaggttga tcggacatat tatcgccgcc aacgtggacc atcagcgcct
     5281 tgtctttgat ttcatccagt gatttcagac gaggcgcgat gacggcatcg gtagctttgc
     5341 cgtcattatt gacgaccagt gcaggcagat cgcctaaatg cccggtacct tctggccctt
     5401 catgtttacc ggtattttgt ggatcaagat gcccgcctgc ggattccgcg gcgctggctt
     5461 tgccatcttt ggtggctggc tggcagcttc ctttggcatg aatatggaag ccatgttcac
     5521 cggggggtaa tgctttcaga tcgggtgaaa actccagacc tttatcggtt tcagtaatgg
     5581 tgacgctacc aattgactgc ccaacccctt gcgacgtgac gaggttcatc tcgacttttt
     5641 cactggcagc ttgtgcgccg gtcgcaacaa ccagcgccag aatggccaga ctaaaacgtt
     5701 tcataggacc tccgttcatt gtgcattcct gaataagtgt aaaccagtga cacatttttg
     5761 aacggcggct attcctaaaa gtgcttacgg tacgtcgtac cccagtgcag ctttacggat
     5821 acgaaaccat tgttgacggg tcattttcag tgtttctgct tcgacagctg cccgtacgcg
     5881 ctcaatttta cctgagccga taattggcag cggctgcgac ggtaaacgta atacccaggc
     5941 gtaaaccacc tgttcaatcg agcccgcgtt taactcctcc gccaccacag ccagttcatc
     6001 acgcagcggc tggaaataat catcattaaa cagacgacca ccaccaaggc aggaccacgc
     6061 catcggacga acacgcagtt gttgtagttg gtcgagcgtg ccatccagca gtaacggctg
     6121 atgcaccggg gatatttcca cctgattagt ggcaagggta aacggcagac gtgattgcaa
     6181 cagggcaaat tgcgcaggcg taaagttcga tacgccaaaa tgacgcactt tgccgctctg
     6241 atgcagatgt ttgaacgcgt ccgccacttc atcggcatcc attaacgggt ctggtcggtg
     6301 gattaacagc agatccagat gatcggtcgc gagattaatt agcgactgtt cggcgctctt
     6361 aatgatgtga ttgcggtcag tgatgtaatg accaatgacg ttttcttcac gcgcggtcgt
     6421 cgcgataccg catttactga cgatttccat ccgttcacgc aggtgaggtg ccagtttcag
     6481 tgcctcgcca aacgccgctt cgcactgata gccaccataa atatcagcat ggtccacggt
     6541 ggtcacgccg agatccagat gctcttcaat aaaactgacc agctggcggg cggacatatt
     6601 ccagtccatc aatcgccagt agcccatcac aaaacgggaa aactccgggc cttccggcgc
     6661 aatagtaata cgctgaacca taatcgcttc ctcttatcag atataagagg agtatacgca
     6721 agattaggtt caaaagagtg atggttgctc cggttcgtct gatgacgctg gcttatttgc
     6781 gcgtaatttg cgcattaatc gctgccgaca aaggcgcagc acctcttgtt tttcgccatc
     6841 gctcatttta ttccagttaa aacgctcatc ccgactacga aaacagccgc gacaaaaccc
     6901 gcgttcgtca gactggcaaa ttccccggca cgggctctgg acgggaaaga actctaattg
     6961 ctccgccacc tcgccctcct cagataagat tattaccatt attgaagctg ttaatgtcca
     7021 aagtagcaac tttgcttgca ctagaccaac tggtctacta cactccaacg catgaacaaa
     7081 cacaccgaac atgatactcg cgaacatctc ttggcgacgg gcgagcaact ttgcctgcaa
     7141 cgtggattca ccgggatggg gctaagcgaa ttactaaaaa ccgctgaagt gccgaaaggg
     7201 tccttctatc actactttcg ctctaaagaa gcgtttggcg ttgccatgct tgagcgtcat
     7261 tacgccgcat atcaccagcg actggctgag ttgctgcaat ccggcgaagg taactaccgc
     7321 gaccgcatac tggcttatta ccagcaaaca ctgaaccagt tttgccaaca tggaaccatc
     7381 agtggttgcc tgacagtaaa actctctgcc gaagtgtgcg atctgtcaga agatatgcgc
     7441 agcgcgatgg ataaaggtgc tcgcggcgtg atcgccctgc tctcgcaggc tctggaaaat
     7501 ggccgtgaga accattgttt aaccttttgt ggcgaaccgc tacaacaggc acaagtgctt
     7561 tacgcactat ggcttggcgc gaatctgcag gccaaaattt cgcgcagttt cgagccactg
     7621 gaaaacgcgc tggcccatgt aaaaaacatt attgcgacgc ctgccgttta gcaggcattt
     7681 tttatcacca gacgaccggg agcctttatg tcatctgaaa aactgtattc cccactgaaa
     7741 gtgggcgcga tcacggcggc aaaccgtatt tttatggcac cgctgacgcg tctgcgcagt
     7801 attgaaccgg gtgacattcc taccccgttg atggcggaat actatcgcca acgtgccagt
     7861 gccggtttga ttattagtga agccacgcaa atttctgccc aggcaaaagg atatgcaggt
     7921 gcgcctggca tccatagtcc ggagcaaatt gccgcatgga aaaaaatcac cgctggcgtt
     7981 catgctgaaa atggtcatat ggccttgcag ctgtggcaca ccggacgcat ttctcacgcc
     8041 agcctgcaac ctggcggtca ggcaccggta gcgccttcag cacttagcgc gggaacacgt
     8101 acttctctgc gcgatgaaaa tggtcaggcg atccgcgttg aaacatccat gccgcgtgcg
     8161 cttgaactgg aagagattcc gggtatcgtc aatgatttcc gtcaggccat tgctaacgcg
     8221 cgtgaagccg cttttgatct ggtagagctc cactctgctc acggttattt gctgcatcag
     8281 ttcctttccc cttcttcaaa ccatcgtacc gatcagtacg gcggcagcgt ggaaaatcgc
     8341 gcacgtttgg tactggaagt ggtcgatgcc gggattgaag aatggggtgc cgatcgcatt
     8401 ggcattcgcg tttcgccaat cggtactttc cagaacacag ataacggccc gaatgaagaa
     8461 gccgatgcac tgtatctgat tgaacaactg ggtaaacgcg gcattgctta tctgcatatg
     8521 tcagaaccag attgggcggg gggtgaaccg tatactgatg cgttccgcga aaaagtacgc
     8581 gcccgtttcc acggtccgat tatcggcgca ggtgcataca cagtagaaaa agctgaaacg
     8641 ctgatcggca aagggttaat tgatgcggtg gcatttggtc gtgactggat tgcgaacccg
     8701 gatctggtcg cccgcttgca gctcaaagct gagcttaacc cacagcgtgc cgaaagtttc
     8761 tacggtggcg gcgcggaagg ctataccgat tacccgacgt tgtaatccaa cattgcgagc
     8821 ggcgtaaagc cgccgctata ctaaaacaac attttgaatc tgttagccat tttgaggata
     8881 aaaagatgcg tcttcttcat accatgctgc gcgttggcga tttgcaacgc tccatcgatt
     8941 tttataccaa agtgctgggc atgaaactgc tgcgtaccag cgaaaacccg gaatacaaat
     9001 actcactggc gtttgttggc tacggcccgg aaaccgaaga agcggtgatt gaactgacct
     9061 acaactgggg cgtggataaa tacgaactcg gcactgctta tggtcacatc gcgcttagcg
     9121 tagataacgc cgctgaagcg tgcgaaaaaa tccgtcaaaa cgggggtaac gtgacccgtg
     9181 aagcgggtcc ggtaaaaggc ggtactacgg ttatcgcgtt tgtggaagat ccggacggtt
     9241 acaaaattga gttaatcgaa gagaaagacg ccggtcgcgg tctaggcaac taatctcctg
     9301 ccgggcgtga actcatcgcg cccgcatctt tactgcatcg acaagtaata tttgtcataa
     9361 tgcgcgctgc aatttatccg tattaagaga atcagatgtc cgataacgct caacttaccg
     9421 gtctgtgcga ccgttttcgt ggtttttatc ctgttgtgat cgatgttgaa acagccggat
     9481 ttaacgccaa aaccgatgcg ctgcttgaga ttgccgccat caccctgaaa atggatgaac
     9541 aaggctggct gatgccggac accacattac atttccacgt cgaaccattt gtcggcgcaa
     9601 atttgcaacc agaagccctc gccttcaacg gcattgaccc gaacgatccc gatcgcggcg
     9661 cggtcagcga atacgaggcg ctgcacgaaa tttttaaagt tgtacgtaaa ggtattaaag
     9721 cgagcggctg taaccgcgcc attatggtgg cgcacaacgc caattttgat cacagcttta
     9781 tgatggccgc cgcagaacgc gcctcgctga aacgtaaccc gttccaccct ttcgccactt
     9841 ttgacactgc tgcactggcc gggctggcac tcggacaaac cgtattgtca aaggcttgcc
     9901 agaccgctgg catggacttc gacagcaccc aggcgcactc cgcgctgtac gacaccgaac
     9961 gcactgctgt gctgttttgt gaaatcgtca accgctggaa acgtctggga ggctggccgc
    10021 tacctgccgc cgaagaggtg taatcgagtc gatgcctgat gacatgcaat gattcaggca
    10081 tctatagtga ggctattcca cgcatcaagc atgatattca cggggaatag cgttaatggc
    10141 agataatcca gacccttcat cgctcctgcc ggacgtgttt tcaccggcga cccgcgactg
    10201 gtttcttcgc gcctttaaac agccgaccgc tgtccagtcg caaacctggc atgtggcggc
    10261 gcgaggcgaa catgcgctgg tgattgcacc gactggctcc gggaaaacgc tggcagcatt
    10321 tctctacgcc ctcgatcggc tcttccgcga aggcggcgaa gatacccgcg aggcgcataa
    10381 gcgtaaaacc tcacgcatcc tctatatttc accgataaaa gccctgggca ccgacgttca
    10441 gcgcaacttg cagatcccgt tgaagggtat tgccgatgaa cggcggcggc gcggcgaaac
    10501 ggtaattatt gatgaagtcc acgcggtagc gggcagtaaa cgtggtgcgc atctggcgtt
    10561 aagtctggag cggctcgatg cgctgctcca cacctcagca cagcgaattg gcctttctgc
    10621 cactgtgcgc tcagccagcg atgtggcagc atttcttggt ggcgatcgcc cggttacggt
    10681 agtcaacccg cccacaatgc gccatccgca gatacgaatt gtcgtaccgg tcgccaatat
    10741 ggatgatgtc tcatcggtcg ccagcggcac cggcgaagac agccatgccg gccgggaagg
    10801 ctccatctgg ccatatattg aaacgggtat ccttgatgaa gtgttgcgcc atcgctcgac
    10861 cattgtcttt actaattcac gcgggctggc ggaaaaactg acggcacgat taaatgagct
    10921 ttacgccgca cgcttacagc gttccccgtc tatcgccgtt gatgcggccc atttcgagtc
    10981 gacctccggc gcaacctcta accgtgtaca aagtagcgac gtttttattg cccgctcaca
    11041 ccacggctcc gtctctaaag aacaacgagc aatcaccgaa caggcgctga aatcgggtga
    11101 attacgctgc gtggtcgcaa cctccagtct tgaactgggg attgatatgg gcgcggtgga
    11161 tctggtgatt caggtggcaa cgccgctttc tgttgccagt gggttacaac gcattggtcg
    11221 cgccggacat caggttggcg gtgtatctaa agggctgttt ttcccccgta cccggcgtga
    11281 tttagtcgat tccgcagtca ttgtagagtg tatgttcgca ggcaggctgg aaaacctgac
    11341 accaccgcat aatcctctcg acgtccttgc gcagcaaacc gttgccgccg cggcgatgga
    11401 tgcattacag gtagacgaat ggtactcccg cgtacgccgt accgcaccgt ggaaagatct
    11461 gccaagacgt gtttttgacg ccacgctgga tatgctttcc gggcgctatc cctctggcga
    11521 tttttctgct tttcgcccca aactggtctg gaacagggag accgggatat tgaccgcccg
    11581 acctggcgct caattgttgg cggttaccag cggcggcacc attccggatc gtggcatgta
    11641 tagcgtgtta ttacccgaag gtgaagaaaa ggccggttcg cggcgggtgg gtgaactgga
    11701 tgaggagatg gtatatgagt cgcgggtgaa cgacattatc actctcggcg ctacctcatg
    11761 gcggatccag caaatcaccc gcgatcaggt gattgtgact cctgctccgg gtcgttctgc
    11821 ccagctcccc ttctggcgtg gtgaaggtaa cggacgtccg gctgaattag gcgagatgat
    11881 cggcgatttt cttcatttac tggcggatgg cgcgttcttt tccgggacta ttcccccgtg
    11941 gctggcagaa gaaaatacga acgccaatat tcaggggttg attgacgagc aacgcaacgc
    12001 gacgggcatc gttccgggga gtcgccatct ggtcctcgaa cggtgccgtg atgaaattgg
    12061 cgactggcgt attattttgc actctcccta tggaagacgg gtgcatgaac cctgggcgct
    12121 ggcgattgcc gggcgaatac atgccctatg gggcgctgac gcgtctgtgg tcgccagtga
    12181 tgacagcatt gttgcgcgca ttcctgacac tgatggtaaa ttgcccgacg ccgcgatttt
    12241 tttgttttaa ccagaaaagt tgctgcaaat tgtccgcgag gcggtaggca gctcggcact
    12301 tttcgccgcc cgttttcgcg aatgcgccgc gcgggcatta ttaatgccgg gccgcacgcc
    12361 gggccatcgc accccgcttt ggcaacaacg gctgcgcgcc agtcagttgc tggaaatcgc
    12421 tcagggatat ccggattttc cggtcattct cgaaacccta cgcgaatgtc tgcaagatgt
    12481 ttatgatctt cccgcactgg aacgtttgat gcgtcgcctg aacggtggcg aaattcaaat
    12541 atccgatgta acgaccacta cgccctcgcc tttcgccaca agtttattgt tcggctatgt
    12601 cgcggaattt atgtaccaga gcgacgcccc gctggcagag cgccgggcat ccgtactgtc
    12661 gctggacagc gagttactgc gcaatctact cggacaggtc gatccggggg aattactcga
    12721 cccgcaggtc attcgccagg tggaagaaga gttgcaacga ctggctcctg gtagaagagc
    12781 gaaaggtgaa gaaggattgt tcgacctgct gcgcgaactg gggccaatga ccgttgaaga
    12841 cctggcgcaa cggcatacag gcagcagtga agaggttgcg tcgtatctgg aaaatcttct
    12901 tgcagtaaaa cgaatcttcc cagcgatgat tagcggacag gagcgtcttg cctgtatgga
    12961 tgatgccgcc aggctgcgtg atgccctcgg cgtacggcta ccagagtcat tgccagagat
    13021 ttatttacat agagtcagtt acccgcttcg cgacctcttt ctgcgctatc tccgggctca
    13081 tgctctggtc acggctgaac aactggctca tgagtttagt ctcggtattg ccattgtcga
    13141 agagcagctt cagcaactgc gtgaacaggg tctggtgatg aatctgcaac aagacatctg
    13201 ggtgagcgat gaagtatttc gtcgtctgcg tttgcgctcg ctgcaagccg ccagagaagc
    13261 gacgcgtccc gttgcagcca cgacctatgc gcgattgctg ctggaacgtc agggcgtatt
    13321 acccgccacc gatggtagcc cggcgctctt tgcctcaaca tctccaggcg tttatgaggg
    13381 cgtagatgga gtgatgcggg tgatcgaaca gcttgccgga gtcggtttac ccgcctcact
    13441 ctgggaaagc cagatcctgc ctgcccgcgt acgcgactat tcgccagaaa tgctcgatga
    13501 attactggca accggagcgg ttatctggtc gggacaaaaa aaagctgggt gaagatgacg
    13561 gcctggtggc actgcatcta cagaaatatg ccgcagaatc gttcactccc gccgaagcgg
    13621 atcaggcgaa tcgttcggcg ctgcagcaag cgataatcgc tgttctggct gacggaggag
    13681 cctggtttgc acaacaaatc agccaacgga tacgcgataa aatcgtcgaa tcggttgatt
    13741 cctcggcact gcaagaggcg ttatgggcgc tggtctggca aggcgtcatt accagcgata
    13801 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    13861 gcagtcaccg ggctcgtcgt gggcgtcctg tctatgcaca acccgtctcg ccgcgggtat
    13921 cttacaacac accaaatctg gctggacgct ggtcgttatt gcaggtggag ccactaaacg
    13981 ataccgaaag gatgctggcg ctggcggaaa atatgctcga ccgctacggc atcatcagtc
    14041 gtcaggcggt gatagccgaa aatatccctg gcgggtttcc atcgatgcaa acactttgtc
    14101 gaagtatgga agactccggg cgaattatgc gaggtcgttt tgtagaaggt ctgggtggcg
    14161 cgcaattcgc tgaacgtctg actattgacc gattgcgcga tctggcgaca caagccacgc
    14221 aaacacgcca ctatacacca gtggcgctct ccgccaacga tccggctaat gtgtggggaa
    14281 atcttctgcc ctgggctgca catccggcaa cgctggtacc aacgcgtcgg gcgggtgcgc
    14341 tggtggtcgt ttctggcggc aaattgttac tctatctggc gcaaggcggc aaaaaaatgc
    14401 tggtctggca ggaaaaggag gaattactcg ccccagagat tttccaggcg ctgaccaccg
    14461 cactgcgtcg cgaaccaagg ctgcgcttta cgctaacaga agtgaatgat cttccggtcc
    14521 ggcaaacgcc gatgtttacg ctgctgcgtg aggcgggatt ttcaagttcg ccacaagggc
    14581 tggattgggg atagttgaca tcctccacgc cctgaaagac gtggttccct gctacgttca
    14641 agctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg actgcaccat
    14701 ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcgc catttacatc
    14761 ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca ggcggttttc
    14821 tttcgctgta tgaccaca
//
LOCUS       DAJVPT010000096        14422 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVPT010000096 DAJVPT010000000
VERSION     DAJVPT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(3..269)
                     /locus_tag="OQM47_003024"
     CDS             complement(3..269)
                     /locus_tag="OQM47_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1481094.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            543..4067
                     /gene="nifJ"
                     /locus_tag="OQM47_003025"
     CDS             543..4067
                     /gene="nifJ"
                     /locus_tag="OQM47_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1481095.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4434..5563
                     /gene="ompN"
                     /locus_tag="OQM47_003026"
                     /pseudo
     CDS             4434..5563
                     /gene="ompN"
                     /locus_tag="OQM47_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5704..6138
                     /gene="uspF"
                     /locus_tag="OQM47_003027"
     CDS             5704..6138
                     /gene="uspF"
                     /locus_tag="OQM47_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1481096.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6314..7249
                     /gene="ttcA"
                     /locus_tag="OQM47_003028"
     CDS             6314..7249
                     /gene="ttcA"
                     /locus_tag="OQM47_003028"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1481097.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEMK"
     gene            complement(7378..8745)
                     /gene="dbpA"
                     /locus_tag="OQM47_003029"
     CDS             complement(7378..8745)
                     /gene="dbpA"
                     /locus_tag="OQM47_003029"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1481098.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9223..10206)
                     /gene="zntB"
                     /locus_tag="OQM47_003030"
     CDS             complement(9223..10206)
                     /gene="zntB"
                     /locus_tag="OQM47_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1481099.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10461..11693
                     /gene="dgcM"
                     /locus_tag="OQM47_003031"
                     /pseudo
     CDS             10461..11693
                     /gene="dgcM"
                     /locus_tag="OQM47_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11714..12277)
                     /gene="smrA"
                     /locus_tag="OQM47_003032"
                     /pseudo
     CDS             complement(11714..12277)
                     /gene="smrA"
                     /locus_tag="OQM47_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12531..13985)
                     /locus_tag="OQM47_003033"
     CDS             complement(12531..13985)
                     /locus_tag="OQM47_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1481100.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13982..>14422)
                     /locus_tag="OQM47_003034"
     CDS             complement(13982..>14422)
                     /locus_tag="OQM47_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1481101.1"
                     /translation="QDEQGRFSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVS
                     LVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIP
                     IENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3836 a         3646 c         3402 g         3538 t
ORIGIN      
        1 aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg
       61 ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg
      121 ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct
      181 acgtgcgccg cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg
      241 gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga
      301 caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc
      361 ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta
      421 ctctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag
      481 gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc
      541 atatgattac tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag
      601 ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg
      661 ccggaaacgg cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg
      721 aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt
      781 ttacgtcatc gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac
      841 tgacgccatt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt
      901 ttggcgatca ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg
      961 ctaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc
     1021 gcgtgccatt tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg
     1081 tcccgctggc cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc
     1141 gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca
     1201 cttatttcca gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg
     1261 aacaggcgat gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt
     1321 acgggcatcc gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg
     1381 aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc
     1441 tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg
     1501 tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg
     1561 tgatgaccgc actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca
     1621 ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat
     1681 ttgccgagct caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg
     1741 tgaccaatct gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag
     1801 ccttgtttta tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga
     1861 ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag
     1921 cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc
     1981 tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga
     2041 tggctgagcg tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg
     2101 aagtgtggtc gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct
     2161 tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata
     2221 ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg
     2281 cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc
     2341 gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg
     2401 taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga
     2461 aaaccgtaac cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc
     2521 cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag
     2581 agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc
     2641 cacactcagc tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca
     2701 gcctgcattc gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg
     2761 tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc
     2821 gtcagaatcc agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag
     2881 agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac
     2941 gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc
     3001 ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt
     3061 tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt
     3121 ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg
     3181 ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc
     3241 tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg
     3301 ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg
     3361 ccgaagcaca tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga
     3421 ttggtggcga cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt
     3481 tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc
     3541 aggcgtcgaa agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta
     3601 aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga
     3661 tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc
     3721 cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc
     3781 tcagccacga ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg
     3841 atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag
     3901 aagcactgga agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc
     3961 cagaagtggc agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact
     4021 tcctagcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa
     4081 ggattatcca attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt
     4141 tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca
     4201 tataacccgc gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat
     4261 tatcttcgat acattcacca gacttattat catttcagag gaattatttg attaaggttt
     4321 tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt
     4381 cactccccga aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa
     4441 gaaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg
     4501 aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt
     4561 atttttctga taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag
     4621 gcgaaaccca aattaacgat caacttactg gctacggtca atgggaatac aatattcagg
     4681 caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga
     4741 aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg
     4801 aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact
     4861 ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg
     4921 taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc
     4981 aggaaggcac caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt
     5041 ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc
     5101 gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg
     5161 ctgggctaaa atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta
     5221 atatgacccc gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag
     5281 ttactagtac cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg
     5341 ccgtgacctt cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct
     5401 ggttaaatac gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt
     5461 tgactataaa atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc
     5521 taccgatgat attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt
     5581 tatggcgggc tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc
     5641 gtcattacac aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt
     5701 tttatgaaca gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc
     5761 gtgattagcc acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg
     5821 gtaatacctt cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg
     5881 gcaatggatg acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt
     5941 aaactgccaa ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt
     6001 ctggaattgg cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat
     6061 atcaccactt atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc
     6121 gtgctggttg tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc
     6181 gcaaatgtgc tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca
     6241 gatttcttat gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt
     6301 gagtcgcttt taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa
     6361 caaattacaa aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat
     6421 tgaagaaggc gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct
     6481 ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa
     6541 cctcgatcaa aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct
     6601 gggcgttgag tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc
     6661 agagggcaaa accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac
     6721 cgcaacggaa ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca
     6781 aacgttgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat
     6841 gagcgatgat ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat
     6901 tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca
     6961 gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc
     7021 agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga
     7081 taccaacctg ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga
     7141 tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga
     7201 tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa atgaaataac caggatagcg
     7261 cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc
     7321 gcatctggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta
     7381 ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca
     7441 tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat
     7501 tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc
     7561 cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag
     7621 cgtcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc
     7681 agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct
     7741 atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag
     7801 ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt
     7861 cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc
     7921 cagatcgccg tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc
     7981 ctggcaatct tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa
     8041 taaccgttgc aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat
     8101 gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc
     8161 gctgattgcg gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc
     8221 aggcgcaaaa cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc
     8281 ggcctcatcc atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg
     8341 atccagcaaa cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg
     8401 ctgcataccg aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg
     8461 cgccagccga cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac
     8521 taaagcctgg gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc
     8581 cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc
     8641 cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt
     8701 gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg
     8761 tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat
     8821 ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg
     8881 ggcactggaa caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt
     8941 gtcgtcatat ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa
     9001 caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag
     9061 taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt
     9121 cacctgcaag ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt
     9181 tgcggcgttt ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg
     9241 gtgcaaccat aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa
     9301 tccgaattgc cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag
     9361 aaaggtactg ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa
     9421 attttcctgc atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc
     9481 gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg
     9541 gtcatcgctc atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg
     9601 tgccatatag cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg
     9661 tggaatttgc tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc
     9721 gataaattca ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca
     9781 atcggtcgga cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt
     9841 gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg
     9901 atcggggcgt tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc
     9961 gaaacggctg actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg
    10021 aagcagcggt gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag
    10081 ccagcaggga tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct
    10141 accatccagc atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc
    10201 ttccactgtg cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac
    10261 gcaacatctc atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata
    10321 caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat
    10381 tatcagaatt atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg
    10441 ataaaaataa cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca
    10501 gtcctgtctg gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc
    10561 gactgttgat gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct
    10621 cacaaaaaga actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa
    10681 tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag
    10741 agctgacaga aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg
    10801 gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct
    10861 tttttctgac taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg
    10921 tcgatgctaa cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga
    10981 aacataccta ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt
    11041 cgcatttacc cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta
    11101 ctcgtcatgt gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat
    11161 gtattgtgca tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc
    11221 accatgacgc gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag
    11281 gccaaatgca gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc
    11341 gttttaaaca cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc
    11401 tcgcccgccc cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg
    11461 aagagtttgt cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa
    11521 ctatccgcgt aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca
    11581 ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg
    11641 atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg
    11701 atgcgtctcg agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct
    11761 gcgccgtttt acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg
    11821 tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa
    11881 tattggcatg tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac
    11941 catccgccag ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct
    12001 ggcgcaaaag attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca
    12061 ccccatgcta caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga
    12121 gaaatccggt agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt
    12181 ggttacgcgt tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca
    12241 tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca
    12301 cgtcgtgatt cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga
    12361 aacaatactc tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct
    12421 gttagcagta tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga
    12481 tttattcata gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa
    12541 ggtaccgaaa atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt
    12601 gaagccagta tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg
    12661 gaaatgaagt cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa
    12721 tgaaaattag ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca
    12781 agattacgtg ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc
    12841 cccagcgtta atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc
    12901 tgtggaaaca gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt
    12961 gaaaacacaa atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt
    13021 aaccagaagt aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa
    13081 cctatctctt cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca
    13141 gtagagaata tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta
    13201 tccttaaaag catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat
    13261 aaaataaaac tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat
    13321 ccccagcgcg ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg
    13381 ataatactcg gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc
    13441 agaaaaggaa tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat
    13501 cgccagtcga ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt
    13561 gaggccagcg cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata
    13621 aacgccatcg tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga
    13681 aacaaaatca tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat
    13741 attcccgcgg atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag
    13801 agcggaataa caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct
    13861 ttacctgcgg acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca
    13921 agctgagcaa taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac
    13981 atcatctctc atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt
    14041 cctttgcgtc gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc
    14101 cagtggcatt ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt
    14161 caatgtgacc gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc
    14221 ctggcttcat tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga
    14281 ctggtactag tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc
    14341 ggtttgcgac aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg
    14401 aaaaccgccc ctgttcgtcc tg
//
LOCUS       DAJVPT010000097        14006 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVPT010000097 DAJVPT010000000
VERSION     DAJVPT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(262..1800)
                     /gene="aldB"
                     /locus_tag="OQM47_003035"
     CDS             complement(262..1800)
                     /gene="aldB"
                     /locus_tag="OQM47_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1481102.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(1965..3116)
                     /gene="yiaY"
                     /locus_tag="OQM47_003036"
     CDS             complement(1965..3116)
                     /gene="yiaY"
                     /locus_tag="OQM47_003036"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1481103.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3306..5150)
                     /gene="selB"
                     /locus_tag="OQM47_003037"
     CDS             complement(3306..5150)
                     /gene="selB"
                     /locus_tag="OQM47_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1481104.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5147..6538)
                     /gene="selA"
                     /locus_tag="OQM47_003038"
     CDS             complement(5147..6538)
                     /gene="selA"
                     /locus_tag="OQM47_003038"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1481105.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6636..7244)
                     /locus_tag="OQM47_003039"
     CDS             complement(6636..7244)
                     /locus_tag="OQM47_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1481106.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7315..8400)
                     /locus_tag="OQM47_003040"
     CDS             complement(7315..8400)
                     /locus_tag="OQM47_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1481107.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8403..8765)
                     /locus_tag="OQM47_003041"
     CDS             complement(8403..8765)
                     /locus_tag="OQM47_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1481108.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9302..11215
                     /gene="mtlA"
                     /locus_tag="OQM47_003042"
     CDS             9302..11215
                     /gene="mtlA"
                     /locus_tag="OQM47_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1481109.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11310..12458
                     /gene="mtlD"
                     /locus_tag="OQM47_003043"
     CDS             11310..12458
                     /gene="mtlD"
                     /locus_tag="OQM47_003043"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1481110.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12458..13045
                     /gene="mtlR"
                     /locus_tag="OQM47_003044"
     CDS             12458..13045
                     /gene="mtlR"
                     /locus_tag="OQM47_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1481111.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13057..13266)
                     /locus_tag="OQM47_003045"
     CDS             complement(13057..13266)
                     /locus_tag="OQM47_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481112.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13550..13912
                     /locus_tag="OQM47_003046"
     CDS             13550..13912
                     /locus_tag="OQM47_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1481113.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3374 a         3770 c         3555 g         3307 t
ORIGIN      
        1 ttacggcctg gttcgcccgc tcaccatcga aacgcgagat taaactcctg acaaaacacg
       61 ccccagaaac gcacttctgt tggcgtagtc tgggttattg cgcacgtagg tttaaaacgt
      121 aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc gtgtacgcct tatccggcct
      181 acggtcaggt tcccgtaggc atgataagac gcgtaagcgt cgcatcaggc aatgaatacc
      241 caatgcgacc agcttcttat atcagaacag ccccaacggt ttatccgagt agctcaccag
      301 caggcacttg gtttgctggt aatgctccag catcatcttg tgggtttcgc gaccgatacc
      361 tgattgtttg tagccaccaa acgcagcatg tgccgggtaa gcgtgataac agttggtcca
      421 cacgcgccca gcctgtatgc cgcgccccat cttataggcc agattaccgt tgcggctcca
      481 gacgcccgcg cccaggccat attgcgtatc gttcgccagc tccagcgctt cttccatcgt
      541 tttgaaggtg gtcaccgcca gcaccgggcc aaaaatctcc tcctggaaca cccgcatatt
      601 gttctgacca aacagaatcg tcggttcgag gtagtagccg tctttcagtt caccttccag
      661 cagcttgcgc cgcccgcctg tgagcacgtc agcgccctct tttttaccga tatcaatgta
      721 gttgaggatg gtttccagtt gcccgtgaga aacctgcgcg cccatttgcg tcacgctgtc
      781 gagcgggtta ccgctgcgaa tgctttcgac acggcggatg gcgcgttcca taaagcgttc
      841 gtagattgac tcctgcacta aggcacgact cggacaggtg caaacttcgc cctggttaaa
      901 ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag gcatcttctt catccatcac
      961 atcagcaaag aagatatttg gcgacttacc gcccaactcc agcgtcaccg gaataatgtt
     1021 ttgcgttgcg tattgcataa tttgttggcc cacttccgtt gagccggtaa acgccacttt
     1081 ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc ccacctgcgc cattgaccac
     1141 gttcaccacg cccggcggca gtaaatcacc gacaatttcc attagcagca gtacagaaag
     1201 cggggtaaga cgtgcgggtt tcagcaccac acagttgccc gccgccagcg cgggagccat
     1261 tttccagctc gccatcagca gcgggaagtt ccacgggata atctgcccca ccacgcctaa
     1321 cggttcatgg aaatgatagg ccacggtttc gctatcaact tcactgatcc caccttcctg
     1381 cgcccgaata cacgaggcga aatagcggaa atggtcaatc gccagcggta catccgcagc
     1441 actggtttcg cgaatgggtt tgccgttatc ccaggtttca gctgtcgcta acagctcgag
     1501 gttttgttcc attcgatcgg caatcttaaa cagaatcgcc gcgcgatcct gcaccgaggt
     1561 gtgcgcccat ttatctttca ctttgtgcgc agcatccagc gccagatcga tgtctcgttt
     1621 gcccgaagac gccacttcgc acagcagctg cccggtcacc ggcgtcagat tctggtaata
     1681 ctcaccatcg gcaggggcta cccattcacc gccaataaag ttgtcgtagc gggcttttaa
     1741 cttgatgggg aaaccatact cgccgggctt aatctgtgct gaagggggat tattggtcat
     1801 gatcgtctcc ttgcggtgtg aggtataaca agggtagacg tgactggcga aatcttcgcc
     1861 agtcggtaac agctttacga cagctatcac gaatttacgg gcaaggatta cagagatagt
     1921 tgaaaaagcg gctgacaatt tgccagccgt tgttggaatg atgattacat tgcggcgcga
     1981 taaatcacca caatttcttc gtgagttgcc tggatcgggt tagtaaagcc acaggcatct
     2041 ttcagggcat tagtcgccag aaccgcgaaa tcttcttctt tcacgttcag gtcgcgtagg
     2101 cctgccggga tatccacttt cttcgccagt tcacggatgg cgttaatgca ggcttcagca
     2161 ccttccgcgt cgtttttacc tgtcacgttc acgcccattg cagcggcaca gtcacgcaga
     2221 cgtgcagcgg cgactttgct gttgaatacc tgaacatgcg gcagcagaac ggcgttacat
     2281 acaccgtgtg gcaggttgta gaaaccgccc agctggtgcg ccatcgcatg aacataaccc
     2341 agagaagcat tattgaacgc cataccggca aggaactggg cataagccat tgcttcacgc
     2401 gctttcgcat tactgccatc ttcaacggct aacggcaggt tttcggcaat catggtcacg
     2461 gctttcagtg cacaagcgtc agtgatcggc gtggcggcaa tagaaacata cgcttcgata
     2521 gcgtgcgtta aggcatccat acccgttgcg gcggtcagtg acttcggcat accaatcatc
     2581 agagaggagt cattgacaga aagcagcgga gtcacgtgct tatcaacaat cgccattttg
     2641 ttatgacgcg cttcgtcagt gatgatgcag aaacgggtca tttcagaggc cgtacccgcc
     2701 gtggtattga tggcgatcat cggcagctgc ggttttgcag agcggtcaac gccttcgtaa
     2761 tcgcgaatat cgccgccatt ggctgccacc agcgcaatac cttttgcgca gtcgtgtgga
     2821 gaaccaccgc ctaaggagat cacgctatcg caattatttt ctttaagtaa tttcaaacct
     2881 gcggcgacgt tttccgtggt ggggttaggt tgggtgccat cataaataac gctaaaaata
     2941 ttgcgttctt ccagtgcttt ttgcacatca cccgccatac ctaatttcgt taacatattg
     3001 tcagtgacaa ttaaggtacg ggtaaatcca taatctgcca tcatattcat tgcatcagtc
     3061 aatgaatcag cgccgatgac attcacagaa ggaataaaga aagttgaaga tgccataaca
     3121 cactcctgaa agtgtaaaaa agggtgggca gcttactgag gattttcatt cgaaaacatg
     3181 atcattatta ttaattatca aaattaatct aataaaaagc acattgtatt cattaaacaa
     3241 tgtgctttat ttaaaatcat aagaaatttg tattaattta ttttaaaaat taattaatca
     3301 tttccttatt tttccggaaa taataatgcg tcgcgcaata aatgatcatt tccacgacga
     3361 cgcgtaaagc caatgcggtc aaaatattcc agaatctgaa ttgccagctt tcggcctacg
     3421 cctaagcgat cgcggaaatc cgccgcgcag gttgaaccac actcctgatc gagatcgcgg
     3481 atcatattgg caaattcgac aatccgatcg ttacggtaat aacgatcttt gacgatcgcg
     3541 gtaattattc cttgctgcgc cgcctggcgt agcgtcaggc gcattgcctg ctcgtcggta
     3601 cccgtctctt ttgccagatc acgcacccac cacggctcgt caccaaacag cggctctgct
     3661 ttttgccaga tggcctgctg ctcttcgctg aagcccgctt tgtgatccgg cagatgcagc
     3721 caaccgtgat gactgaggat gtcgccgctt tcgcgcatct tttcaatcag caacagtact
     3781 agcgcttcat cttccattgg caacgccata cgtcgcagac gttcgcgccc aggcccaggt
     3841 tcatcgcgat gttgctcatg ataagtcgct aatgtgtcga gaattttccg ttgccagcga
     3901 gcggcaaccg gcgcattcaa caagctataa ccagcctgaa tatagccagg ctgttgcagc
     3961 agttcgcgca tcccttcgcc gttgagctgg cgcgcccaag cgaaatcagg aagattaacc
     4021 gcgccgcgtt gcaggtgaac ggctaacgct tcgctatcat tctgtgccct ggcgagggaa
     4081 gccagccatt gcagatactc tggtttacgt ttaccgcgac gcggcgggtt aagcatcacg
     4141 acgcgcgctc cggccagcgt gttgcgggca gagatatcgc gcaataccag gcggtcgtta
     4201 tctgccagcc ataacggggt gtcgaagacc agttcagcaa ggttatcttc cagcagtgaa
     4261 acgcgtcccg tgacgtggct ggcggcgtgg tgaatatgca gcggctgcca ctgggtcagc
     4321 ggtgcatggg tttgaagctc gacaatcacc cgtgtgaacg gctctggcgg cacatcggca
     4381 agcagccagt cgccacggtt aatctgctct ttttccgcat cacccgcgat gtcaagcgcg
     4441 atacgctgcc cggcattggc ggtttctgtt ggctggtttt gcgcatgcag cgcacgtaca
     4501 cgcatcggtt tatttacacc agtcagccag agtgaatcgc ctaccttcac ttccccgctt
     4561 aacgccgtac cggtgacgac cagcccggca ccttttacgg taaatgcgcg gtcaatcgcg
     4621 aggcggaaac tatgttggct ggcgtgctcg cgttccggca actgaagcag atgctcgcgc
     4681 agggcatcaa ttccccgacc ttcggttgct gcggtgataa acagttttgc ctcagcaaaa
     4741 ccgtattccc gcagaacctc ctttacctgg cgttcaacct catcaacacg cgcttcgtcc
     4801 acgcgatcgg ctttggtcag cgccactgtc agcatcgggt taccggtcag ctgcaaaatc
     4861 gccagatgct cacgggtctg tgccatcacg ccgtcatcgc acgccaccac caacagcgcg
     4921 tgatcgatac caccaacgcc cgccagcatg ttggaaagaa acttttcatg accgggaacg
     4981 tcgataaaac caggcacgcg accatccggc tgcggccagt aggcatagcc gagatcgatg
     5041 gtcatgccgc gctttttttc ttccggcaga cggtcagcat ttacgccagt aatcgcctgc
     5101 aataaggttg ttttgccgtg gtcaacgtgt ccggcagtcg caataatcat ttcaacaaca
     5161 tctccaaaaa ccgttgctca tcttcaaggc agcgtaaatc cagccacaat cgtccgtcat
     5221 aaatacgacc aatcaccggc actggcaatt cacgccagcg ggcggctaat gactcaaggt
     5281 ggctaccgcg tccatcatgg ggtgtaaacg ttaatgccgc gctcggcagg cgatcaaccg
     5341 gcagcgaacc actgccaatc tgcgaaagac atggcataac ctgtaccgca aactccgcgc
     5401 cgtaatgtgc gacaagggga gcctgtaaac gttgtgcctg gatttgaatg acctctgcgc
     5461 tgcgggtaag caggcgcagg gtcggtaatt tttcactcag agcttcaggg tgtaaataaa
     5521 gacgcaacgt ggcttccagc gccgcgaggg tcattttatc cgcgcgtaat gcacgcttca
     5581 gcgggtggct ttgcaggcgg gcgatcatct cttttttacc aacgataatc ccggcctgcg
     5641 gcccgcctaa caacttgtcg ccggagaagc tcaccagact gacgcccgcc gcaatcaact
     5701 cctgcggcat tggctctttc ggcaaaccgt actggctaag atcgaccagc gagccactgc
     5761 ctaaatcagt caccactgga acatccagct ctttgccgag cgccaccagt tcagcttcgt
     5821 ctatcgcttt ggtgaacccc tgaatgctgt agttactggt atgtactttc atcaacagtg
     5881 cggtattttc attcaccgcc tgacgataat cattcgcgtg cgtgcggttg gtggtcccta
     5941 cttcgtgtag ggtgcagcct gcctgacgca taacatcggg aatacgaaac gcgccgccaa
     6001 tctccaccag ttcgccgcga gataccacca cctctttccc gctggcagtg gccgccaaca
     6061 tcaataacac cgccgccgca ttgttattga cgatacaggc atcttccgcc cccgtaatac
     6121 ggcacagcag ctgcgccagc gcccgatcgc gatgtccgcg tccggcgtcg tccagatcgt
     6181 actcgagggt cactggcgaa cgcatagcct gcgcaacggc ttccaccgcg gcttccgcct
     6241 gtaaagctcg cccaaggttg gtatgcagca cggttcccgt caggttgatc accggacgca
     6301 gcgcgctctg cgcctctttc gtcaaccggg catcgacttc ttgcgcccag ttttcacacc
     6361 acgcaggcag cgtctggctg ccacgaatca cttctcgcgc ttcgtcgagc atctgacgca
     6421 acaattccac cacgcgggtg tgaccataag tatcacgcaa agaaaggaag gagctatcgc
     6481 gcaataagcg atcaatagcc ggaagttgac tatagaggga acgcgtttcg gttgtcatag
     6541 gaaacctggc tgatcaaggc cctctcacac ggagaagggc gtttaacata accacggatt
     6601 gtaacgtgag atgggtcagg aggacatatc gcgcatcaag cctttggcgg ttcggtgcga
     6661 gcaaaacttt cgcggctaaa caggttttcg accagtttga ctaagtgtgg gcggtcgaca
     6721 caccaacctg gcgcaacgcg gcggaaattg agatatccga cagcacaggc aatggcgata
     6781 gtcgccagat tgaccgtatc ggttttgagt gtgccatcga ccagatatcc ttccagcaca
     6841 tccagactgc ggttgatttt ctcccgctgg cgtaacaatt catcttcaga ctgctgcgcc
     6901 gccggacgcg cctgttcacg caccgatacc agcccggcat ccataatgcc atccgccagc
     6961 gcctcaattt tgcgcacccg caacgactcc agcggatcgc gcggcaacat cgccggagcg
     7021 acattcatta actcaatata ttcagcgatg atcggcgaat caaaccagca ttcgccctct
     7081 tcggtcacca gcaccggcac tttccctaac gggttaaatt gcgccacgcc gttatccgcg
     7141 ttatagggca gttcattaat aaattcgaaa gttatgccct tttctaacaa cagaataaaa
     7201 agtttgcgta caaacgggct ggtgtagcta ccgacgagtt tcatgccgag tcctttgtgc
     7261 gaggaaaaat atcagtatgg ctcaggctca cgcaaaacac aggtgatata agtctcaatg
     7321 atccaaataa agataatgca tccaactggt cattcttagc agcactttcc gcatcaccga
     7381 aacatggctg aaatggtcgg agtaaaccgc cacctgggcg tagatgccgt cgggtaaggc
     7441 atcgatatca tcgttagggt ccagttcaat ggttcccagc acaccgtccg tgccgggaac
     7501 gaccgttaat gattgcaata ccccctgcgc ctgataagaa ccgcctggca cgacaggtaa
     7561 aatactagtc aatttgccgt ggaacacctg cccaggtagc gcgttaaaca ccacttccgc
     7621 atcatcgcca ggtttcagac gtaacagcga gttttgccga aattgggcga caatttgccg
     7681 tttttgctcg gggatgaaga ccatcaccgg acgcagcggc aaggcagctg cgtatgtacc
     7741 tgggcggatc agtacctgag tgacgtagcc attgcttggt gcgcgaatga cagtctgctc
     7801 aagattatat tttgcttcag taagttgcgc tcttaagctc acaatctgcg attgctcgcc
     7861 gttgaccata ctgtcgagct gactctggat ctgcgcctgc tccgccaccg agcctttcac
     7921 taacgcatcc tgcgcgagga aattttgccg cgcatcgtcg atgtcacgtt ccgagaacgg
     7981 attcaccgcc gcctggctgc ctttcaggta acgttgataa tttttaaaca gacggtcgcg
     8041 ctccgctgaa acctgggtgg tgttggcctg cgcttcagtg agctgcgcac gcagcgtctt
     8101 tatattatgc gtcgccgtca tcaggtcagc ctgaagtctg tcaactcgcg cctggtaacg
     8161 aaccgggtcg agcttaaaaa gcacctcgcc cttttgaata agctgattat tcttgtcagt
     8221 gacttcagta acaattcccg tcacctgtgg cgtgataggg atcgctatca ctgccttttg
     8281 cgcggtaaaa gtgtaagggt ggttgtagtt catcaacaaa atcaaaccac tcaccagaaa
     8341 cacgcctccc agcgccgccg tcgccagcgc cacgtaagtt aaaacaatca atagatccat
     8401 aattagatct ccgggaacgt ggaatagtca gtctttttct cggcggcgag ttgatgctcc
     8461 agccgggaaa ttcggtcaga aagtgcggcg atttccggtt cagtcgcttt ctcctgcgac
     8521 gcaacatgtg actgcatacc ccagccacgc tccggttgat agagcgtcgc ccagatccac
     8581 agaaacggcc agataacatg cagagtaaac aggctcaccc agcccgccgt atgaatagcg
     8641 tcggcatggg ggtgattgcg ctttttggca atcagataag ggatgtcatg tatcgcgatg
     8701 attccataaa aaatcaccag aaagacgaag atcagcactc ccaacgcgaa atagtttaga
     8761 aacatatctg cctcggattc acgtttatca gtgttgtttt ttggctggca gccagaaggg
     8821 agtcaggctg atattttgac aataatccgg gttcgcgatt ctcgccataa caccaaagaa
     8881 taatttttag aggtgatgag ttgcttagtt acataacgat tgtatgacga aggcataaca
     8941 tgctgtagat cacatcaggt gaacgccgta agaaaatatc ttgtgattca gatcacaaag
     9001 attcaacaaa ccatcaaaac aaaaatgtga cattactcac atttaaatgc catttttagc
     9061 gaaaatcacc gccttgtttc ttttttatac aggcgttttg tgatgagcgt cacgtcaatt
     9121 acctctctac cccctatatt tatgtgactg atatcacaca aaagcccgtc gactggacag
     9181 ttaaccgatt cagtgccaga tttcgcagta tctacaaggt ccggctacct ctgccgccac
     9241 attaacaaaa aacctcgggc ttccagcctg cgcgacagca aacataagaa ggggtgtttt
     9301 tatgtcatcc gatattaaga tcaaagtgca aagctttggt cgtttcctca gcaacatggt
     9361 gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat ttattccaac
     9421 agggtggtta ccgaacgaga cgctggcgaa gctggtcggg ccgatgatca cttatctcct
     9481 accgctgctg atcggttata ccggtggtaa gctggtaggc ggcaaacgtg gtggcgtagt
     9541 cggtgccatt accaccatgg gcgttatcgt cggcgcagac atgccgatgt tcctcggttc
     9601 tatgattgca ggtccgctgg gcggctggtg cattaagcac ttcgaccgct gggtagacgg
     9661 taagatcaaa tccggttttg agatgctggt gaataacttc tccgcaggca tcatcgggat
     9721 gatcctcgct attctggcat tcctcggcat tggcccgatt gttgaagccc tgtccaaaat
     9781 gctggctgcg ggcgttaact tcatggttgt ccatgacatg ctgccgctgg cgtctatctt
     9841 tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt aaccacggta tcttctcgcc
     9901 gctgggtatt cagcagtccc atgaactggg taaatcaatc ttcttcctga ttgaagctaa
     9961 cccaggtcca ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa
    10021 acagtctgcg ggcggtgcgg caatcatcca cttcctgggt ggtatccacg aaatctactt
    10081 cccgtatgtg ctgatgaatc cgcgtctgat cctcgcagtc atcctcggcg gtatgactgg
    10141 cgtgttcacg ctgactatcc tgggcggtgg tctggtttct ccggcatctc cgggttctat
    10201 ccttgctgta ctggcgatga caccaaaagg tgcttatttc gctaacatcg cgggtgtgtg
    10261 tgcggcgatg gctgtctcct tcgttgtctc tgctattttg ctgaaaacca gcaaagtgaa
    10321 agaagaagat gatattgaag cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa
    10381 aggcacatct ccgctatctg cgggcgatgt gactaacgac ctgagccacg tacgtaaaat
    10441 catcgttgcc tgtgacgccg gtatgggttc aagtgcgatg ggcgcaggcg tgctgcgtaa
    10501 gaaaattcag gatgcaggtc tgtcgcagat ttcggtcact aacagcgcga tcaacaacct
    10561 gccgccagat gtggacctcg tcatcactca ccgtgacctg accgaacgcg ctatgcgcca
    10621 ggttccgcag gcacagcata tttcgctgac caacttcctc gacagcggcc tgtacaccag
    10681 cctaaccgaa cgtctcgttg ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga
    10741 cagtctgaaa gacagctttg acgattccag tgctaacctg ttcaaactgg gcgcggagaa
    10801 catcttcctc ggtcgcaaag cagcaaccaa agaagaagcg attcgttttg ctggcgagca
    10861 gctggtgaaa ggcggttacg ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa
    10921 actgaccccg acttatctgg gtgagtctat cgcggtgcca cacggtacgg ttgaagcgaa
    10981 agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg
    11041 tgaagaagaa gatgacattg cccgtctggt gattggtatt gcagcccgta acaacgagca
    11101 cattcaggtt atcaccagcc tgaccaatgc actggatgat gagtccgtca tcgagcgtct
    11161 ggcacacacc accagcgtgg atgaagtgct ggaactgctg gcaggtcgta agtaatccaa
    11221 tcctaccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
    11281 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
    11341 tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg
    11401 atgtcaatca ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg
    11461 tcggtgaaac cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg
    11521 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg
    11581 tgctggaacg tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta
    11641 atgaatcccc gctgaacatc atcgcctgtg aaaacatggt acgcggcacc acgcagctga
    11701 aaggtcatgt gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gagcacgttg
    11761 gctttgtcga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
    11821 cgctggaagt gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag
    11881 gcgcactgcc gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac
    11941 gtaaactctt caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg
    12001 gtcatcagac cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg
    12061 cgatggaaga aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg
    12121 cggcgtacat ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag
    12181 agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
    12241 tgctcggtac gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg
    12301 caatgcactt ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg
    12361 acaaaggtcc gcaggcggcg ctggcacaga tttccggtct tgatgccaac agcgaggttg
    12421 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac
    12481 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggccgt
    12541 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
    12601 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
    12661 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga
    12721 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
    12781 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
    12841 ctttaccgac gacgaaatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc
    12901 accgccgcca cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta
    12961 tcaacaggct gtgcgatcaa caatggtcct ttcactgact gagctgattt ccaaaatcag
    13021 cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg
    13081 ccgataaagc cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg
    13141 tattccggac ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg
    13201 gcttgatgcc atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt
    13261 gcccatgatg atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt
    13321 taggttcccg gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct
    13381 gctgtttatt atctttttct atagatttct taattaatca acgaattgcg tagtttttga
    13441 gcatgttaat gattacgttg cagaactatt acaatgtcat cagttgtgaa caaagcacct
    13501 ggtcgcgcat actaggggct ataaatttat ctttatcaga agccatcaca tgaaagaagt
    13561 cgaaaaaaac gaaatcaagc gtctcagcga tcgcctggac gccatccgcc accagcaggc
    13621 cgatctgtcg ctggttgaag ccgcagacaa atatgccgag ctggaaaaag agaaagccac
    13681 gctggaagca gaaattgctc gcctgcgtga ggttcatagc caaaaactga gtaaagaagc
    13741 acagaagctg atgaagatgc cgttccagcg cgcaattacc aaaaaagagc aggctgatat
    13801 gggcaagctg aagaaaagtg ttcgcggact ggtggttgtg cacccaatga ccgcgctggg
    13861 gcgcgaaatg ggcctggaag agatgactgg attttctaag actacgtttt aagcccctca
    13921 gacgactggt tcgatcgtat caacccgcat ctggcttgcc ttagctggtc aatgcgggga
    13981 ctctcctgtc tactcgccta attacc
//
LOCUS       DAJVPT010000098        13875 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVPT010000098 DAJVPT010000000
VERSION     DAJVPT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            92..217
                     /locus_tag="OQM47_003047"
     ncRNA           92..217
                     /ncRNA_class="other"
                     /locus_tag="OQM47_003047"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            337..678
                     /locus_tag="OQM47_003048"
     CDS             337..678
                     /locus_tag="OQM47_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1481114.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(669..1595)
                     /locus_tag="OQM47_003049"
     CDS             complement(669..1595)
                     /locus_tag="OQM47_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1481115.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1685..2683
                     /locus_tag="OQM47_003050"
     CDS             1685..2683
                     /locus_tag="OQM47_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1481116.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2680..2898)
                     /locus_tag="OQM47_003051"
     CDS             complement(2680..2898)
                     /locus_tag="OQM47_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1481117.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2900..4915)
                     /gene="ligA"
                     /locus_tag="OQM47_003052"
     CDS             complement(2900..4915)
                     /gene="ligA"
                     /locus_tag="OQM47_003052"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1481118.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4986..5972)
                     /gene="zipA"
                     /locus_tag="OQM47_003053"
     CDS             complement(4986..5972)
                     /gene="zipA"
                     /locus_tag="OQM47_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1481119.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6202..6963
                     /gene="cysZ"
                     /locus_tag="OQM47_003054"
     CDS             6202..6963
                     /gene="cysZ"
                     /locus_tag="OQM47_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1481120.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7148..8119
                     /gene="cysK"
                     /locus_tag="OQM47_003055"
     CDS             7148..8119
                     /gene="cysK"
                     /locus_tag="OQM47_003055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1481121.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8503..8760
                     /gene="ptsH"
                     /locus_tag="OQM47_003056"
     CDS             8503..8760
                     /gene="ptsH"
                     /locus_tag="OQM47_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1481122.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8805..10532
                     /gene="ptsI"
                     /locus_tag="OQM47_003057"
     CDS             8805..10532
                     /gene="ptsI"
                     /locus_tag="OQM47_003057"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1481123.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10573..11082
                     /gene="crr"
                     /locus_tag="OQM47_003058"
     CDS             10573..11082
                     /gene="crr"
                     /locus_tag="OQM47_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1481124.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11124..11975)
                     /gene="pdxK"
                     /locus_tag="OQM47_003059"
                     /gene_synonym="thiJ"
     CDS             complement(11124..11975)
                     /gene="pdxK"
                     /locus_tag="OQM47_003059"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1481125.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12080..12454
                     /locus_tag="OQM47_003060"
     CDS             12080..12454
                     /locus_tag="OQM47_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1481126.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12487..13210
                     /locus_tag="OQM47_003061"
                     /pseudo
     CDS             12487..13210
                     /locus_tag="OQM47_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13414..>13875)
                     /locus_tag="OQM47_003062"
     CDS             complement(13414..>13875)
                     /locus_tag="OQM47_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCS1481127.1"
                     /translation="YTTTGPEIWQQTGGRITHFVSSMGTTGTITGVSCFMREQSKPVT
                     IVGLQPEEGSSIPGIRRWPAEYLPGIFNAALVDEVLDIHQRDAENTMRELAVREGIFC
                     GVSSGGAVAGALRVAKANPGAVVVAIICDRGDRYLSTGVFGEEHFSQGAGI"
BASE COUNT         3465 a         3563 c         3377 g         3470 t
ORIGIN      
        1 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
       61 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac
      121 ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga
      181 agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc
      241 gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc ttgttcttct
      301 accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc ttcaacttca
      361 attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga tgttgcttca
      421 caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa agagattgta
      481 gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca acagcaaatc
      541 accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag
      601 aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat
      661 atcgatatct atatctgata caacggatcc ccttcccgac cagccattag cgcattgccc
      721 tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg gttcttcctg
      781 cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta
      841 ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc tgcggctacc
      901 atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg aataccattg
      961 cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat
     1021 ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc gctatcgcga
     1081 tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc gcccactggg
     1141 cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc catcacccac
     1201 tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg gctttcagca
     1261 atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg aacttttccg
     1321 ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc atccagcagt
     1381 cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt
     1441 ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg acttactgcc
     1501 gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg cgctactgtg
     1561 acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga atggatgcaa
     1621 taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat catctggagt
     1681 ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca cggtggtgtt
     1741 gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa atctgaccac
     1801 cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat
     1861 tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt
     1921 tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta
     1981 ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa tcgccttcac
     2041 gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct
     2101 ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg gtgcaagggg
     2161 cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg tgttggggca
     2221 tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac
     2281 tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg tcgttaatgg
     2341 tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca gctgcgttct
     2401 tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct tcaataaggc
     2461 agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat
     2521 ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc tgatgatttt
     2581 ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac gccagaccga
     2641 acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac gcttcagggg
     2701 ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca ttagctcacc
     2761 gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact cctccggcag
     2821 gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg ctatttcaat
     2881 cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct tcgtcgatga
     2941 cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca cccgctatca
     3001 ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg accagtcgag
     3061 ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg gttttaccag
     3121 caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag tgaacacctt
     3181 ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt
     3241 gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc gaagcggctt
     3301 ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc tcgccgactt
     3361 cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct ttttccagcg
     3421 cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc agtttgcctg
     3481 cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc agctgatcga
     3541 tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg
     3601 actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc gcttcacctt
     3661 ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca acctcacggg
     3721 tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg tcgccagcgc
     3781 ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca tccgcattgt
     3841 gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga cgcgcaacag
     3901 gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag gtcatctgct
     3961 cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca aagccaagct
     4021 gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg
     4081 tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc gattcacaaa
     4141 gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa cggccaagat
     4201 gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa aaagtgagcg
     4261 gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc gcattacgtg
     4321 ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg
     4381 cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc tctccgtgca
     4441 gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc ccggtggtgc
     4501 catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata ctgacggcaa
     4561 ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc agacggtcct
     4621 gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc agtgacagca
     4681 ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct acacgttggg
     4741 taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc agttcgcgca
     4801 tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga taaagatatt
     4861 catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat tccatatcgc
     4921 accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa
     4981 gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc gcgcaatttc
     5041 tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc atcggcaata
     5101 tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta agacggtacc
     5161 tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc aaaggttccc
     5221 ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg gctaagatga
     5281 cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct gttaagaagc
     5341 agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat caccgcttct
     5401 ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg ctgtggtgcc
     5461 gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc
     5521 tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc aggctgcggc
     5581 tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg cggtacctgc
     5641 gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggtaggtac
     5701 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
     5761 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
     5821 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
     5881 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
     5941 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
     6001 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
     6061 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
     6121 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
     6181 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
     6241 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
     6301 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
     6361 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
     6421 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
     6481 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
     6541 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
     6601 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
     6661 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
     6721 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
     6781 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
     6841 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
     6901 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
     6961 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
     7021 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
     7081 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
     7141 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
     7201 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
     7261 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
     7321 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
     7381 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
     7441 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
     7501 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
     7561 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
     7621 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
     7681 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
     7741 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
     7801 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
     7861 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
     7921 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
     7981 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
     8041 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
     8101 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
     8161 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
     8221 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
     8281 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
     8341 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
     8401 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
     8461 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
     8521 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
     8581 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
     8641 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
     8701 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
     8761 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
     8821 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
     8881 ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt tttctgagcg
     8941 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
     9001 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
     9061 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
     9121 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
     9181 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
     9241 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
     9301 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
     9361 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
     9421 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
     9481 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
     9541 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
     9601 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
     9661 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
     9721 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
     9781 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
     9841 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
     9901 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
     9961 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    10021 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    10081 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    10141 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    10201 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    10261 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    10321 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    10381 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    10441 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    10501 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    10561 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    10621 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    10681 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    10741 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    10801 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    10861 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    10921 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    10981 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    11041 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    11101 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    11161 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    11221 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    11281 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    11341 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    11401 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    11461 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    11521 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    11581 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    11641 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    11701 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    11761 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    11821 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    11881 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    11941 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    12001 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    12061 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    12121 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    12181 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    12241 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    12301 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    12361 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    12421 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    12481 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    12541 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    12601 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    12661 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    12721 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    12781 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    12841 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    12901 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    12961 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    13021 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    13081 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    13141 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg cttttccaca
    13201 cgaaaattaa aaactgcatt tgtcggcagc aacaactgcc gacaaatcag attattgaca
    13261 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    13321 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    13381 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    13441 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    13501 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    13561 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    13621 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    13681 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    13741 aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga tagtgccggt
    13801 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    13861 cccggtggtg gtgta
//
LOCUS       DAJVPT010000099        12948 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVPT010000099 DAJVPT010000000
VERSION     DAJVPT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..2246
                     /locus_tag="OQM47_003063"
                     /pseudo
     CDS             1..2246
                     /locus_tag="OQM47_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2256..5902
                     /locus_tag="OQM47_003064"
                     /pseudo
     CDS             2256..5902
                     /locus_tag="OQM47_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            5963..6370
                     /locus_tag="OQM47_003065"
     CDS             5963..6370
                     /locus_tag="OQM47_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481128.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            6912..8000
                     /locus_tag="OQM47_003066"
     CDS             6912..8000
                     /locus_tag="OQM47_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1481129.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8011..10290
                     /locus_tag="OQM47_003067"
     CDS             8011..10290
                     /locus_tag="OQM47_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481130.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10283..11420
                     /locus_tag="OQM47_003068"
                     /pseudo
     CDS             10283..11420
                     /locus_tag="OQM47_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            11417..>12948
                     /locus_tag="OQM47_003069"
     CDS             11417..>12948
                     /locus_tag="OQM47_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481131.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     ED"
BASE COUNT         3192 a         3411 c         3339 g         3006 t
ORIGIN      
        1 aaagaaaaaa tcgccgattc cggtgctgga tttagcgcca ttaggcatcg agccaatctg
       61 ttatctgaca gaagaaatca gtaatcaact tttggcgaaa tatatctggt attcaaaaca
      121 catcacggtt agccatgaag aaagtactac caacctgttg gcaaggatgg gttttcaacg
      181 acggatcgct ggtacatata ttaaagctcc cgaagcggta gttgaggcat ggctaaatga
      241 agattattca accttactaa gtgaatttaa ggtgtttcat tcacctaccg ggcattattg
      301 gcagttgggg attttgacaa cattgccgct ggaggctgga gaaagcagta aaagcatgga
      361 atgcccttac tctatctcca cataccgata ccgaatacgc catgttacat tttggactca
      421 aaggtttacc tggtttagtt aactcaatta cacgctatcc acaggaagcc ttgcccatca
      481 cgaattactt cgcagcgagt gagctggctc ctgccgtcgc ccgtgccttc aacaaactca
      541 aaacgctacg cgaaaacgcc cgcacctggc tgttgaaata cccggaacat gcccttaccg
      601 gcctgctgcc tgcggcgctc ggcaaagccg gtgaagcaca ggataacgcc cgcgctgcct
      661 tgcgtatgct taccgaaaac ggtcatcagc cattactgca agaaatcgcc cggcgttata
      721 accagtcgga agtaacagac gcggtgaacg ctctgcttgc gctcgatccc ttagataatc
      781 acccgacaaa aatccccact cttccgacct tttatcagcc atcgatctgg acgcgcccgg
      841 tattaaaagc aaacgcccaa tcactgccag ataacgccct cctccacctc ggcgaaatgc
      901 tccgcttccc tcaggaagag gctctgtatc cgggattatt gcaggtgaaa gacgcctgta
      961 ccgctgactc tctggctgaa tttgcctggg atctgtttac cgcctggcag accgctggcg
     1021 cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt tctcggtaac gatgacaccg
     1081 cccgcaaact gacgccatta atacgcgcct ggcctggtga atcccagcat aaacgcgcca
     1141 ccgttgggtt ggatattctc gccgctatcg gtagtgatat cgccctgatg cagcttaacg
     1201 gcattgctca gaaactgaaa ttcaaagcat tacaggagcg ggcaaaagaa aagattgccg
     1261 acattgccga gagccgcgaa ctcacggtag cggagcttga agatcggtta gcaccggatc
     1321 tcggtctgga tgataacggt tcgttgctgc tggattttgg cccacgccag ttcaccgtca
     1381 gctttgatga aaccttaaaa ccgtttgtgc gtgatgtttc cggcagccgc ctgaaagacc
     1441 tgccaaaacc aaacaaaagc gattatgagt cacaggcgaa cgatgctgtt aaccgctaca
     1501 aattgctgaa aaaagatgcg cgtaccgtcg cggcccagca ggtagcaagg cttgaatccg
     1561 ccatgtgcct gcgtcgccgc tggtcgccgg aaaacttcca actcttcctg gttgtgcatc
     1621 cgctggttcg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc gacaaccagc
     1681 tactggcctg tttccgggta gcggaagata acagctacag caccgctgac gatgatcttt
     1741 tcaccctgcc ggaaggcgat atctctatcg gcattcctca cgttctggaa atatcaccga
     1801 cggatgctgt cgcctttggt cagctttttg ccgactacga actgctacca ccgttccgtc
     1861 agcttgaccg taacagttac gccctgacag aagccgagcg caacgccagc gaactgaccc
     1921 gctgggcagg cagaaaatgc ccgagcggcc gggtcatggg gctggcaaat aaaggctgga
     1981 taaagggcga accacaggat ggaggctgga tgatcaaacc tctcggtcgc tggtcgttaa
     2041 tcatggaaat cgacgaaggt tttgcggtgg gtatatcacc agccgaactt agcgctgagc
     2101 agctcttaag caagctgtgg ctatgggaag gcaaagcgga aagctatggc tgggggagta
     2161 attcaacaca ggaagcgcag ttctccgtac tcgatgccat caccgccagc gagctaatta
     2221 acgatattga agccctgttt gaataaggaa agagcatgga caaggaatta ccgtggctgg
     2281 cggataacgc ccaactggaa ctgaaatata aaaaaggcaa aacaccactc agtcatcgca
     2341 attggccggg cgaaccagtg cctgttatca ctgaaagcat cattcagaca ctgggtgatg
     2401 aattgctaca aaaagctgag aagaaaaaaa acattgtctg gcgttatgaa aatttttcac
     2461 cggagtggca gtccgccatt acgcaggcca tcaacttgat cggcgaacac aaaccctcag
     2521 tcccggcccg cacaatggcg gcactagcct ttatcgcgca aaatgacaac caacagttgc
     2581 tcgacgaaat cgtccaacaa gaggggctgg aatatgcgac tgaggtggtg attgcacgcc
     2641 agtttattgc acggtgttat gagagtgatc ctctggtagt cacattgcag tatcagaacg
     2701 aggactatgg ctatggttat cgctcagaaa cctataacga attcgatctc cgactgcgta
     2761 aacacctctc tctggcagag gaaagctgct ggcagcgttg cgccgacaaa ctcattgccg
     2821 cactgccagg aataaccaaa gttcgccgcc cttttattgc gctgatcctc cccgaaaaac
     2881 cagaaatcgc caatgagttg gtaagccttg aatgcccgcg gactcatttt cattctaagg
     2941 agtggttaaa agttgttgct actgacccca aagcggtgag aaaactcgaa cgctactgga
     3001 gccaggatat atttagcgat cgagaagcca gctacatgtc gcatgaaaac cacttcggct
     3061 acgcggcctg cgctgccctt ttgcgcgaac aaggactggc agtcgttccg cgcctcgcga
     3121 tatatgccca taaagaagat tgcggcagtc tgctggtaca aattaaccat ccgcaagtca
     3181 tccgcacctt gctgttagtt gctgataaaa acaaacccag cctgcaacgt gtggctaaat
     3241 acagtaaaaa cttcccccat gcgacactcg ccgcactggc agaactgctg gcgttaaaag
     3301 aaccaccagc ccgcccaggt tatccaatca tcgaagacaa aaagctgcct gcacagcaaa
     3361 aagctcgaga tgaatactgg cgtacgctgt tacaaacgct gatggcatcg cagccacaac
     3421 tggcagcaga agtgatgccg tggttaagta ctcaagcccg ggcagtgctg aatagttatt
     3481 tatcggcacc gcccaaacca gttattgata gcaccgataa cagccaaatg cctgaaatcc
     3541 tcgtttcacc accatggcgt gggagaaaga aaaagacttc catacgtcac ttttcgctgc
     3601 ctgatcttac gcttgcaccg caaacgtaca ggtcgccgaa taaacactgg acgccggaac
     3661 agcaaactgc aattgaccat tttagcgcca tgtcttttga cgaacgtctt gctacgagag
     3721 gagcggatat cttcttgcgg gaattaggtt ttgacgttca tagcctggaa tttaataagc
     3781 gtcttcttaa tggacagttg gatgcctttg atgctcagca actgcaagaa tacacatcaa
     3841 taggactatt gcgtacttat cacgcgcatc aggccaaatc taaacagtac cgggaagacg
     3901 ccaccaatgc attactggca caagatagca ttgcgttgat gggagggtgg cgaacttttc
     3961 agcacggatt ttactatggt aatgaacagc gttgggggat ttggaatctg taccttatcg
     4021 cgcagatgcc gcgcgaaatg gcggtatcgt gctggcaaca aattgtcgag gctgattttc
     4081 attacaccgg ggtggaatat ttgttaagtg tgctgggtac cgatgctttg cctggattaa
     4141 acaccgcctt tgcccgacat ccaaaagaga tatttccgct gctgattcat ttcggcgcaa
     4201 cagaactggc cctgcccatt gcccgcgtct ggcacctttt tgcgggccag cgtaatctgg
     4261 ctcgccagtg gattttacaa tggccggaac atacggctac tgcacttatc cctcttgtct
     4321 tcaccaaatc tagcgataaa agcgaagctg cattacttgc cctgcgttta ctttacgaac
     4381 atgggcatgg cgaatggcta caaactgtag ccaatcgttg gcagcgtaca gatctatggc
     4441 ctgccctgga acaattactt aaacagagtc caatagagat ttacccggca cgcattccaa
     4501 aagcccctga tttctggcat ccgcaaatgt ggcccaggcc gcgccttatc actaataatc
     4561 aacctgttac cgatgacgct ctggaaatta tcggcgaaat gctgcgcttt acccaggggg
     4621 gacgttttta tagcgggctg gaacaactga aaacgttctg ccagccacaa acgctggcag
     4681 cttttgcctg ggatctcttc actgcgtggc aacaagctgg tgcccccgca aaagacaact
     4741 gggcatttct ggcgttaagt ctctttggtg acgaaagcac ggcacgggat ctaacgacac
     4801 atatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc ggtcagtggc atgaacatcc
     4861 ttaccctgat gaataatgat atggcgctga tgcagctgca tcatatatcg caacgggcga
     4921 aatcccgccc cttacgtgat aacgcggcgg aatttcttca ggtagtcgca gaaaatcgcg
     4981 ggctaagcca ggaagagtta gcggacagat tagtcccaac actgggcctt aatgatccgc
     5041 aggcgttgag ttttgatttt ggtccccggc agtttaccgt tcgcttcgat gaaaacctca
     5101 acccggttat ctttgatcag caaaacgttc gccagaaaag tgttccccgg ttgcgcgccg
     5161 atgacgatca actgaaagcg cccgaggcac tggcccgact aaaagggtta aaaaaagatg
     5221 ctactcaggt gagcaaaaac ctgctcccgc gtcttgaagc tgccctacgt accacccgac
     5281 gctggtcgct ggcagatttt cattctctgt ttgttaatca tccctttacc cgtctggtta
     5341 cccagcgatt aatatggggg gttatccggc aaatgaaccg cgtcgtttac tcaacgcctt
     5401 tcgtgtggcc gcagaggggg agttctgcaa tgcgcaagat gagccaattg acctgcctgc
     5461 ggacgctctg attggcattg cccacccgtt agaaatgaca gcagaaatgc gcagtgaatt
     5521 tgcacagctt tttgccgatt acgaaattat gccgcctttt cgccagttgt cgcgccgcac
     5581 ggtgctgctc acacctgacg agtcaaccag taacagcctg actcgctggg aaggtaaatc
     5641 cgctaccgtt gggcaactta tgggaatgcg atacaaaggc tgggagtcag gttatgagga
     5701 cgcatttgtc tatgacctgg gtgagtaccg gctggtcctt aagttttcac ccggttttaa
     5761 ccactacaat gttgatagca aagcgctaat gagcttccgt tctcttcgag tgtaccgtga
     5821 caataaatcc gtcacttttg ccgaacttga tgtgtttgat ttgagtgagg cgttaagcgc
     5881 acctgacgtc attttccatt aaaacaaagc gggcagtgat gcgactgccc gttatctaca
     5941 cgacttacca gcggggaaag cgatgatcgt gcaaaaagag ctggttgcta tttacgatta
     6001 tgaggtccct gtacctgaag atccgttttc cttcagactt gagatccata aatgctctga
     6061 attatttaca ggttccgtct accgactgga acggttccgg ctacgtccaa catttcatca
     6121 acgtgatcga gaggatgttg acccgctaat aaatgatgcg ttgatttata taagagatga
     6181 gtttattgat gagcaaaaat tacgaggcga atcacctgaa cctgtaatag taatttttaa
     6241 ttgtaaacta cagaatatat tcaaccaaca aatagaatta tatactctag ataattcaag
     6301 tcgcagcaca tgcaacttga agtatgacga gtatagcttt attaaagggg ggaacggtat
     6361 tcccccttag atgcgtttca atttgcccgt tacttcaaca aatcaatcaa ccgtaacaga
     6421 gccactaata gctccagtac agcaatgagc acggttaact tttgtgtcag cgtgtccatc
     6481 cgctctcctt attaaggagc acaacaccag caccttaccc ttacactgcc tgtcgatact
     6541 ggtttacgtt tgttgagtcg ccatgttgca ctccggatca gagcactacc tgcggcacca
     6601 cccgtccggt agccaggact tgaaataggt tcgatccggc gcaacttccc aacagtcagg
     6661 attataaatc tcattatctt aattgcaaca ctgtccagag gtggttataa ttcgttcgtt
     6721 ggaacacgat gttgcacagg cagtggtgtt aggcctgaaa atagtaaata aaagaacctg
     6781 cctcaccagc agtttttttt atttactgtg atctgctttc cagatatttt tcgctcaaac
     6841 aactaatgcg ccaaacattt attgcgcgta aaatatcgtt tatttcatta atacatttca
     6901 gggatgaata tatgtcaccg cagaataatc atctgcagcg tccgcctgct gctgtgttat
     6961 acgccgatga actggcaaaa ttaaaacaaa atgataacgc accttgcccg cccggttggc
     7021 agttaagttt gcctgcggcc cgtgctttta tccttggcga tagcgcgcaa aatattagcc
     7081 gtaaagtggt gattagtccc tccgctgtcg aacgtatgtt agtcactctt gccaccggac
     7141 gcggtttgat gttggtgggg gaacctggca ccgcaaaatc tctcctgtct gaattactgg
     7201 caacctctat tagcggcgat gccgggttaa ccatccaggg cggcgcatct actaccgaag
     7261 atcaaatcaa gtacggctgg aactacgccc tgcttatcaa tcatggtcca tcaacggaag
     7321 cgctggtccc tgccccgctt taccagggaa tgcgcgacgg caaaatcgtc cgttttgaag
     7381 agataacgcg tacaccgctg gaagtgcaag actgtctgtt aggtatgctt tctgacaggg
     7441 tgatgacggt gccggaactc actggtgaag ccagtcagct ctatgcgcga gaaggcttta
     7501 atattattgc cactgccaat acccgcgatc gcggcgtcaa tgaaatgagt gctgcgctaa
     7561 agcgtcgctt cgattttgaa actgtctttc cgattatgga tttcgcccag gagctggagc
     7621 ttgtcgccag tgcttcggcg cgtttgctgg cccatagcgg tattccgcat aaagtgcctg
     7681 acgcggtact ggagttactg gtccgcacct tccgcgatct gcgtgccaac ggcgaaaaga
     7741 aaacgtcaat ggatacacta acagcgatta tgtccaccgc tgaagccgtg aacgtggccc
     7801 acgccgtagg cgtccgcgcc tggtttttag cgaatcgcgc aggtgagcca gccgacctgg
     7861 tggactgcat tgccggaacc atcgtcaaag ataacgaaga agatcgcgct cgtctgcgcc
     7921 gttacttcga acagcgtgtc gccacacata aagaagctca ctggcaggct tattatcaag
     7981 cccgccaccg cctgccgtga ggaaagatgc atgagcgagc cgttaattgt cggcatccgg
     8041 catcatagtc cggcctgcgc ccggctggtg aaatcgttaa tcgaaagcca gcggccacga
     8101 tacgtgttga ttgaaggccc tgctgatttt aatgaccggg tagacgaact gtttttagcc
     8161 caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc agcccccggg
     8221 cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct acaagccgat
     8281 cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca gagtgaagaa
     8341 gaggacgatt cgcctgatac gcaagaggaa agccagacct tgctgctgcg tgccacccgc
     8401 atggataaca gcgacaccct gtgggatcac ttgttcgaag atgaaagcca gcaaactgca
     8461 ttaccctctg cactggcgca ctattttgcc caactgcggg gcgactcccc cggcgatgct
     8521 ctaaatcgtc agcgcgaagc ctttatggcc cgctggattg gatgggcgat gcagcaaaat
     8581 aatggcgacg tgttagttgt ctgcggtggc tggcacgctc cggcactggc aaaaatgtgg
     8641 cgcgaatgtc cgcaggtaat taacacgcca gaattgccgt cgctggcaga tgccgttaca
     8701 ggttgttatc tcacccccta cagtgaaaag cgccttgatg tgctggcagg atacctttca
     8761 ggaatgcctg ccccggtctg gcaaaactgg tgctggcagt ggggcttaca gcaggcggga
     8821 gaacaactgc taaaaacggt tctcacccgt ttgcgccagc acaagttgcc tgcttcgacc
     8881 gcggatatgg ctgccgctca tctgcatgcg atggcactgg cacagttgcg cggtcataca
     8941 ctaccgttac gcactgactg gctggatgcc atagcaggat cgctgattaa agaagccctg
     9001 aatgcgccgt tgccgtggag ctatcgcggc gttattcatc ccgataccga tccgattctg
     9061 ctaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc ttctacgcca
     9121 caaccgcctc tgccaaaaga tgtcacttgc gaactggaac gtaccgcaat ctctcttccg
     9181 gcggagctta ccttaaatcg ctttaacccc aatggactag cgcaaagtca ggtgttacat
     9241 cggctggcaa tactggagat cccagggatt gtacgccagc agggaagcac actgacactt
     9301 gcaggcaacg gtgaagaacg ctggaaatta acccgcccgc ttagccagca tgcggcattg
     9361 attgaggccg cctgctttgg tgccacactc ctggaagccg cacgccataa attagaagcc
     9421 gatatgctgg acgcgggtgg aatcggcagc attaccacat gtcttagcca agcggcgtca
     9481 gcgggtctgg cgtccttcag tcaacaatta ctggagcaac tcacattatt aatcgcccag
     9541 gaaaatcaat ttgccgaaat gggccaggcg ctggaagtgc tatatgcctt atggcggctg
     9601 gatgaaatta gcggtatgca aggcgcgcag atattacaaa cgacgttatg cgcggctatc
     9661 gatcgcacgc tgtggctgtg tgaatccaac ggcagaccgg atgaaaagga gtttcacgct
     9721 cacttgcata gctggcaagc gttgtgtcat atcctgcgcg atctacatag cggtgtgcaa
     9781 ctccccggcg tttcgctttc tgcggcggta gccttgctgg agcgacacag tcaggcgatt
     9841 catgtcctgg cactggatcg cggcgcgact cttggtgcgc tgatgcgtct ggaacatccc
     9901 aacgctagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc acaatccggc
     9961 gaggcgctgc acggtttgct ggcattagcc cgtcatcaac tggcctgtca gccggcattt
    10021 atcgccggtt tcagcagtca tttaaatcaa ctaagtgatg ccgattttac caatgccctg
    10081 cccgatttac gcgcggcaat ggcctggcta ccgccacgag aacgcgggac gctggcgcat
    10141 caggtgcttg agcattatca actggcgcaa cttcccgttt cggcactgca aatgccgttg
    10201 cattgtccac cgcaagccat tgcacatcat caacaactcg aacagcaggc actggcatcg
    10261 ctgcaacact ggggagtttt ccatgtctga actgaacgat cttctgacca cccgtgagct
    10321 acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg ggctggatga
    10381 caacgcccgg cagatagatc acgcgctgga gtggctgtat gggcgcgatc ctgaacggct
    10441 ccagcgtggt gaacgctcta gtggattagg tggctcaaat ctcaccactc ctgagtggat
    10501 caacagtatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa gcgatgccgt
    10561 actgcgctac ggcattgaag atgtggtgac aaatctcgac gtgctggaac gtatgcagcc
    10621 ttccgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaacc ccgaagtact
    10681 ggctgccgcc cgcaggattg tgcgccaggt tgtggaagaa attatggcgc gactggcaaa
    10741 ggaagttcgt caggcttttt ctggtgtccg cgatcgtcgt cgccgctcat ttattccact
    10801 ggcgcgaaac tttgatttca aaagtactct gcgcgccaac ctgcaacact ggcacccgca
    10861 acacggcaag ttgtatatcg aattcccccg ctttaacagc cgcattaaac gccaaagcga
    10921 gcaatggcaa ctggtcttac tggttgatca aagcggatcg atggtcgatt cggtgatcca
    10981 ctctgcggtg atggcggcct gtttatggca gttacccggc attcgtaccc atctggtggc
    11041 gtttgaccac aagcgtcgtt gatctcacgg cagacgttgc cgatccggta gagttattaa
    11101 tgaaagtgca attaggcggc ggaaccaata tcgccagtgc catggagtat ggtcggcaac
    11161 ttattgaaca acctgcaaaa agcgtcatta tcctcgtgag cgatttttat gaagggggtt
    11221 catcatcatt gctgacgcac caagtgaaaa agtgtgtcca gagcggcatc aaagtgctgg
    11281 gactggcagc gctcgatagc acagcaacac cttgctatga ccgcgatacg gcccaggcgc
    11341 tggttaatgt cggcgcacaa atagccgcca tgacaccggg cgagctggca tcatggcttg
    11401 cggagaatct tcagtcatga attcactacg tccggaatta ttagaactga caccgcaggc
    11461 actgacggcg ttaagcaatg ccggttttgt taagcgcagc cttaaggaac tggaaaatgg
    11521 caacgtcccg gaaatcagcc atgagaacag cgctttaatc gccaccttca gtgacggtgt
    11581 ccgtacccag ctggcgaacg gccaggcact gaaagaggct cagtgcacct atggggccag
    11641 cggcatgtgc cgtcatcgcg tgatgctggt gttaagttat caacgacttt gtgctaccgc
    11701 ccagcctacg ggaaaagaag aggaatggga tccggcaatc tggctggaag aactggctac
    11761 ccttcccgat gccacccgca aacgcgcaca ggcgctggtc gctaaaagca tcaccattga
    11821 gttgttctgt gcgccgggtg aaattccctc tgcccgctta ccgatgagcg atgtgcgttt
    11881 ttattcccgc agcagtattc gtttcgcccg ttgtgattgt attgaaggca cactttgcga
    11941 acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa gcgcagcaag cggaattgac
    12001 tcatttaatc tggcagatgc gcagcgaaca cgtcacatca tctgacgatc cgtttgccag
    12061 cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta agccaggcat tatggctgag
    12121 cggcatcagc cagccgctta ttcactacga ggccgctttc agccgcgcgc agcaagcagc
    12181 ggaacgctgc agctggcgat gggtgagtga atcgctacgg caactgcgcg ccagcgttga
    12241 tgccttccac gcccgcgcca gcgttgatgc cttccacgcc cgcgccagcc actatcatgc
    12301 cggagaatgc ttacgtcagc ttgcggcatt aaacagtcga ttaaattgcg cacaagagat
    12361 ggcccggagc gacagtgttg gtgaagttcc tcctgtgccg tggcgcacgg tcgttggctc
    12421 tggcattgcc ggagaagcaa agcttgatca tctgcggctg gtgtctttag gtatgcgttg
    12481 ctggcaggat attgagcatt atggtttacg catctggttt accgatcccg acaccggcag
    12541 tattttgcac ctttcgcgca gttggccgcg aagtgaacag gaagactcac cggcagctac
    12601 gcgtcggctg tttagttttc aggctggcgc actggcgggt gggcaaattg tttcacaagc
    12661 agcaaaacgc agtgccgatg gcgagctgct gttagctacc cgcaaccgct taagcagcgt
    12721 tgtaccgctg tcgcctgatg cctggcaaat gttgagcgcg ccgttacgcc agccgggcat
    12781 tgtggctttg cgggaatatt tacgccagcg tccccccgcc tgcatacggc ctcttaatca
    12841 ggtcgataac ttatttattc tgccggtcgc tgagtgtatt tcgctcggtt gggatagcag
    12901 ccgccagacg ctggatgcgc aggtaatcag cggcgaaggt gaagataa
//
LOCUS       DAJVPT010000100        12810 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVPT010000100 DAJVPT010000000
VERSION     DAJVPT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1176)
                     /locus_tag="OQM47_003070"
     CDS             complement(<1..1176)
                     /locus_tag="OQM47_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1481132.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OQM47_003071"
     tRNA            complement(1375..1459)
                     /locus_tag="OQM47_003071"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OQM47_003072"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OQM47_003072"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1481133.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="OQM47_003073"
     CDS             2803..4305
                     /locus_tag="OQM47_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1481134.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OQM47_003074"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OQM47_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1481135.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OQM47_003075"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OQM47_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1481136.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="OQM47_003076"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="OQM47_003076"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1481137.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="OQM47_003077"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="OQM47_003077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1481138.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="OQM47_003078"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="OQM47_003078"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1481139.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..>12810)
                     /locus_tag="OQM47_003079"
     CDS             complement(11870..>12810)
                     /locus_tag="OQM47_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1481140.1"
                     /translation="AILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLRYQALMTSLNG
                     VRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGI
                     VSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGY
                     IIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNH
                     FMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTY
                     LLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3242 a         3328 c         3373 g         2867 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ctcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctatttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgccagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgg ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtcaggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg acgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcacaccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ccgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gcgctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcga
//
LOCUS       DAJVPT010000101        12620 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVPT010000101 DAJVPT010000000
VERSION     DAJVPT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..424)
                     /locus_tag="OQM47_003080"
     CDS             complement(<1..424)
                     /locus_tag="OQM47_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1481141.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVD"
     gene            665..1465
                     /locus_tag="OQM47_003081"
     CDS             665..1465
                     /locus_tag="OQM47_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1481142.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1543..2313
                     /locus_tag="OQM47_003082"
     CDS             1543..2313
                     /locus_tag="OQM47_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1481143.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2361..3707)
                     /gene="mltD"
                     /locus_tag="OQM47_003083"
     CDS             complement(2361..3707)
                     /gene="mltD"
                     /locus_tag="OQM47_003083"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1481144.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(3779..4534)
                     /gene="gloB"
                     /locus_tag="OQM47_003084"
     CDS             complement(3779..4534)
                     /gene="gloB"
                     /locus_tag="OQM47_003084"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1481145.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4568..5290
                     /locus_tag="OQM47_003085"
     CDS             4568..5290
                     /locus_tag="OQM47_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1481146.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5287..5754)
                     /gene="rnhA"
                     /locus_tag="OQM47_003086"
     CDS             complement(5287..5754)
                     /gene="rnhA"
                     /locus_tag="OQM47_003086"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1481147.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5819..6550
                     /gene="dnaQ"
                     /locus_tag="OQM47_003087"
     CDS             5819..6550
                     /gene="dnaQ"
                     /locus_tag="OQM47_003087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1481148.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6683..6759
                     /locus_tag="OQM47_003088"
     tRNA            6683..6759
                     /locus_tag="OQM47_003088"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6717..6719,aa:Asp,seq:gtc)
     gene            complement(6890..7936)
                     /locus_tag="OQM47_003089"
     CDS             complement(6890..7936)
                     /locus_tag="OQM47_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1481149.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(7950..8509)
                     /gene="tssG"
                     /locus_tag="OQM47_003090"
                     /pseudo
     CDS             complement(7950..8509)
                     /gene="tssG"
                     /locus_tag="OQM47_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8506..10389)
                     /gene="tssF"
                     /locus_tag="OQM47_003091"
     CDS             complement(8506..10389)
                     /gene="tssF"
                     /locus_tag="OQM47_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1481150.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10405..10899)
                     /gene="tssE"
                     /locus_tag="OQM47_003092"
     CDS             complement(10405..10899)
                     /gene="tssE"
                     /locus_tag="OQM47_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1481151.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(10896..11726)
                     /locus_tag="OQM47_003093"
                     /pseudo
     CDS             complement(10896..11726)
                     /locus_tag="OQM47_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(11713..12615)
                     /gene="tagK"
                     /locus_tag="OQM47_003094"
                     /pseudo
     CDS             complement(11713..12615)
                     /gene="tagK"
                     /locus_tag="OQM47_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         2889 a         3275 c         3371 g         3085 t
ORIGIN      
        1 catccacttt tctttcgatc aaattaataa tcgtttcgga ggagactagc gataaagtga
       61 cttccggata gcgttcacgg aaaggcttaa ttaacggcat cagaaagtgc agcaccactg
      121 gagttgcggc atcgatccgt aacagtccac gcggtgtatt acgcgtctcc ataatttctg
      181 attctgccgc tgccatctcc tgcaaaattg actgtacgcg acgaaaataa cgctcgcctt
      241 cttccgtcag gctaagttgc cgcgtggtcc gattaagcag gctaacgcca agtttcattt
      301 ccagcttttt caccgcccgg cttaccgctg agtttgcttg ccctaattgt tccgctgccc
      361 ggctaaagct gccgctttcg acgaccgaaa caaaaatggc gagttcttcc gacgtggctt
      421 tcatttttgc tcctgttgca aaatagatga gatattttga atttatttgt cattaaacca
      481 tcaggatgcg tgatatgtca tccgatttaa tgttctcaat aatgagcaaa tttctgacag
      541 gtgtaagcac ttgcttacat aacaatatac aattgctcgt tgaaagagtg agctaaaatc
      601 cctataacag tagaaccctc ccgagtgcgg aagggttgac gtaatagagg tttcaaagtc
      661 aaaagtgcga aaaaacacct atgccatgcg ctatgttgcc ggacaacctg cggaaaggat
      721 cttaccgccg gggtcttttg cgagcatcgg ccaggcatta ccacctggag aaccgttaag
      781 taccgaagag cgtattcgga tcctggtgtg gaacatatac aaacagcaac gcgctgaatg
      841 gttgtcggta ttaaagaact acggcaaaga tgcacatctg gtgttattgc aggaagcgca
      901 gacaacgcca gagttagtac agtttgcgac cgctaactat cttgccgccg atcaggtgcc
      961 cgctttcgtg ctgccacaac atccttccgg tgtaatgacc ctttcggcgg cgcatccagt
     1021 gtattgctgc ccgttacgtg aacgagaacc cattttgcgt ctggcgaagt cggcactggt
     1081 gacggtctat ccattacctg acacccgcct gttgatggtg gttaatatac acgccgtcaa
     1141 cttcagtctg ggcgtggatg tctatagtaa gcagttactt cctattggtg atcagatagc
     1201 tcaccacagc ggcccggtca ttatggcggg agatttcaat gcctggagcc gtagaaggat
     1261 gaacgcgtta tatcgctttg cgcgggaaat gtcgctgcgc caggtgcgtt ttaccgatga
     1321 tcagcgccgt cgggcgtttg gccgcccgct cgattttgtt ttctaccgtg gtctgaacgt
     1381 cagcgaagct tctgtattgg ttacgcgcgc ttccgatcac aatccgctac tcgttgaatt
     1441 cagtcccggc aagcctgata aataaggtat gtcaggtctg ccacagggca gaccaacgtt
     1501 tggcgctgtc cttttatact caaacaacaa aaggacagcg caatgacaac acaatcccac
     1561 catgaccacg tagaaaagca gtttagctct caagcctgcg aatatctgac cagtaccgta
     1621 catgcatccg gacgagattt acagcgcctg gcggtgcgtc tggctgatta tcctggtgca
     1681 agtgcacttg atatgggctg cggagcaggg catgccagct ttgttgctgc gcaaaacgtg
     1741 agcgcggtgg tggcgtatga cttatctgcc caaatgctgg atgtcgtggc acaagctgcc
     1801 gaagcccggc aactgaaaaa tatcaccacc cgccagggat atgccgaaag tctgccattt
     1861 gccgataacg catttgatat tgttatcagc cgttattctg cccatcactg gcatgatgtt
     1921 ggtgcagcac tgcgtgaagt gaaccggata ttgaaacctg gcggtaggat gattgtgatg
     1981 gacgtaatgt ctccgggtca tccagtgcgc gacatctggt tacagacggt agaagcatta
     2041 cgcgatacct ctcacgtacg aaactacgcc agcggtgagt ggttgaggtt aatcaatgaa
     2101 gccaatctga tagttgataa tttaattaca gataagttac cgctggaatt ttgttcatgg
     2161 gtcgcgagaa tgcgtacgcc agaagcgtta gtagacgcta ttcgcattta ccaacagagc
     2221 gcatcgacag aggtgagaac gtattttgcc ttgcagaatg acggctcttt caccagtgat
     2281 atcatcatgg tagaagcaca taaagcggca taaataaaaa aggcaccggg ggaatcggta
     2341 cctttttatt atctggtttg tcaggaatct ggcatgttgt tgtttttcac aaacaacgtc
     2401 agcttgtcgc ctggttgcag attcgcagtg tcgctgttcc agcgcatcac atctttgatg
     2461 ttcacgccgt ggcgtttagc aatgcttgaa agcgaatcgc ctttgcgcac acgatacgta
     2521 atgctatcgc tgttgtttgc cagtcgctgt gcaccaatcg tcaaactttg gcctggcttc
     2581 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     2641 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     2701 gtattgttgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     2761 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     2821 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     2881 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     2941 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3001 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3061 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3121 ttataagccg ctacggtcag aagccagtcg tcgtcaaaca ttttgttcag acgctgcatc
     3181 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3241 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3301 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3361 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3421 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3481 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     3541 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     3601 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     3661 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     3721 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     3781 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     3841 tttcttcatt aattccatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     3901 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     3961 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4021 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4081 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4141 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4201 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4261 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacactacaa
     4321 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4381 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4441 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4501 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     4561 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     4621 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     4681 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     4741 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     4801 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     4861 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     4921 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     4981 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5041 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5101 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5161 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5221 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5281 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5341 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5401 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5461 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     5521 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     5581 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     5641 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     5701 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     5761 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     5821 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     5881 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     5941 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6001 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6061 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6121 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6181 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6241 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6301 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6361 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6421 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6481 gctggcagca catgaagccc gtctcgatct ggtgcagaaa aaaggcggaa gctgcctctg
     6541 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     6601 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     6661 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     6721 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     6781 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     6841 ggatatgatt taaaaaatgg ccagacatta taaattctgg ccacttctgt tactccagcg
     6901 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     6961 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7021 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7081 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7141 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7201 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7261 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7321 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7381 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7441 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7501 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     7561 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     7621 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     7681 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     7741 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     7801 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     7861 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     7921 acaggaatat ttccatttaa ttatcctcag tgcgccagcg tggtggccag accgtccgga
     7981 ttgctggact cacgcaccag atgggccgat gccgccagac gagcgtgatc tggcaacggg
     8041 ctgtcagcgc tctcccgcaa atcctgcccg ctgaggctgg cgacataaaa ggcaaagcgg
     8101 tcatgttgcg gtttatccag accgccccca gcggactggg cgctgcgcca tgcctggtaa
     8161 aactgcgtca gccagcggtg atgaaaaaga tcagaaaaac gggtcagcgt cggctttcag
     8221 tccggttacg cgccagttcg gtgtagtgca gcggcagtgg accgttcggc ccccacagca
     8281 cgaggctgta cagactgatg tgcaggcgcc cttcctgcca gcccacactg gcgatttctc
     8341 gtggcgcaaa ggtcagcgcg ggcgtttgtc cgaggcgaaa tttttccatc cgtggttgcc
     8401 aggtgttgcc cgccggaacg gtacacaact gcacatccac gcggcgcatc aggttcagaa
     8461 aaccgtaacg ccaggggggg gcctgcgcct gagtgaaatc gttgctcata cgcttccccg
     8521 ctgtcccgcc ctgacaggcc aggtcataat tttgccgcgc tgcatgctgt gcagcgtcat
     8581 ctgcgagaag gtattaatgg aaacatggcg ggcgatatag tgttccagca ccaggccgaa
     8641 cagatacgga ctgatgccgg aaaaaccttc ttcatccacc gtcagttcac aactgacgcc
     8701 acgcccatag accagtagtc cggaaccggg cagacggcgg gtgaccgggg tcgttttaca
     8761 accaatcagg ctgcgcacct ggcgcgattg cggactgtca tgagcaggaa taaacaggtt
     8821 cagcagatct cgcaacgcct ggccgccggt acggtgatcc agatcggcca gcggcagata
     8881 attaaaagat aactggcgga tcaaccgcca tgccatctca cgttcagcca tcgggggctg
     8941 cggcgagcgt ggcgggcgga taagccctac gcccgccacc gggatggctg catccacggt
     9001 tagatcgtcc cggccattgc gggcaataag acagggcaga tcgcggttag tgaccatggc
     9061 cgtgatggtg atgtggcgca gattttccgg gtacggcgct tcatactggt caaccagcga
     9121 caggaacact tccgagcccg tatagggggt acgggtgccg taacggcggg cgttttctga
     9181 taagcggcgg ggctcccggc gcagggaaaa ataacgtccg tgattgcctt catcattatt
     9241 gcgggtgtgg tagagcgggc gaaaggccat cttccgtgtg gtgtcggttt ccagcccttc
     9301 cacttcctgg accgagaaca cctcataatc cagcggatgg gttctgtcca ccaccagatg
     9361 ctgttccgtc gtgctgtgcg tgacatcgat ccgggcggtg acgcggggaa acaggttgat
     9421 aaccggggta cagaacaggc tgaactgtgc cgcatttgtc tggtggatca gccagtccgg
     9481 cggcaggcgg ttcagcagga taacaatctc cgccacgcca ccgtcaattt tctgtaatcc
     9541 ggcagacagg cctgtcgggg tgaaaaaata gaagcgttcc ggacaggcaa aatattcatg
     9601 cagcaggtta tgaccgtgaa acacgttcca cgccagcggc agcagcccct gatccggctc
     9661 caggccttcg tacataacgg gctgttgcag attcacatcc agcgcgccgt caaaatgccc
     9721 cggaatgcca gccaggggcg cgatggcgct ggtatgtaga agttctaaca ggtgtgaggc
     9781 cgtacgctcc tcgccgcaga gataaaacgg cagcctgtcc agtccggcaa gctgactgaa
     9841 cgttagttca ccaaaggtgc gcagggtaat acgcagcgcc ccggcgacgt gaatgttggc
     9901 cggcaggtag cggtgcaggg caggcatatc cggtggcgcg gcggtcagtc gcgcctcttc
     9961 gatggcgagc ggccacagcg ttacatcctg gctgctgcga aactggcagg cagtgctttc
    10021 gccttccgga atggcggaga aaaaggctgt gtcgcgcggg acggtaaatc ctttcgccag
    10081 atcgccttct tccgtatcgg gatgcaactg cgccacgctc attgagggcg tgggtgtgac
    10141 gtaattgggg ctgaccactt ccagcaggcg ctgggtgaaa cgggggaatt cagcgtcaat
    10201 ttttagctgc gtgcgggcgg tcaggaagct gaaggcctcg accattcgct ctacatacgg
    10261 atcggcaata tccgttccct gcatgcccag ccgggcggcg actttgggat gacgggcggc
    10321 aaattcggct cccgtttccc gcagatagct cagttcgcgg ttgtagtatt ccagtagccg
    10381 tggatccatc ggtctctctc tgaattaaaa aaacgtaatg cgactctgct ccatatccag
    10441 cgcgctacgt acccggaatg cagtcggata cggctgcgtg aggatctcgc cgcgaatttc
    10501 aaactgtaat gtgttatggc cactctggcg gcgctcatcc tgaagtggga tgatttgcag
    10561 agtggacgct ttcagccggg gttcaaaccg ggtgatggtt cgcaaaatgg ccctgcggat
    10621 atcgtcccac ttgtgctcat acagaaagct gcccgccagt ggcggcaggc cgtagttaag
    10681 tacggacgct gccgcgtacg gataacgtgc agcatcaata tcatcttcat ggctgacggt
    10741 gttgagcaga aatgacaaat cccggcggat gatctcctta atctgaaccg gactgacgct
    10801 gataccgtgg tcgcgtttct ggtttggggc gtcgtcacac aggcggtcaa acagcgtggg
    10861 cagcaggtga ttgcctggac gctggcggga agcgctcacg cgtcgcttcc ttcagcggtg
    10921 ttaaaggtac aggtggacaa atccagcagg ctgatatcgc cgtgaccggt gagccacacc
    10981 ctctgaccca gcgcctgtac cagtgtctct cccggaccgt cctgccagac cgtttcccgg
    11041 cacagacgca gggagtcggc agccttttca gagccgctgt agcggggaaa cagctaggca
    11101 ctgtgtgtat cgccattgtt cagggtgacg ttgacgggtt tccagatcaa atcgatcaga
    11161 tgcgcaggcc gcggcgattt cagcgaacag atttgtgaaa acggcagcca gatatatgct
    11221 ccgcccgcga tcagttcgag taccggaccg gtacgggagt cgctgtcgct aatccagtca
    11281 aatgcgccgc cattccactg cccggaagtg tctgttatgg cctcaagcgc gcggctgcgg
    11341 tgagcatcag cttcctgcgc ttcaccacgg gcgttacagg cgagggcggt cagcagatcc
    11401 tccatccagg cgggcggcgg gagaataaca ccgggccgct gttcgccctg aaagcaggcg
    11461 tggcggtata tctcacagcg gatcagctcg ccaaatacct gcgcttcgcg agtataattt
    11521 gcgtccatcc gggcgcatag ctggatctgt tgcagtgccc gcgaccagtc gcctgccacg
    11581 cacaataact gaaacaggct gtggcggcgg gaggcctgcg ctgggtgcat cctgacctgt
    11641 tgctctgcca tctgaatgcc ctccaccacg gaataatccc gcagcagcgc cgacaaggta
    11701 atcggtagct cgttattgtt tgtcatgata tatctctcca tgttgtgccg ggtttatccg
    11761 gtaatggctg tcaatgccaa tgatgcgatg ctctttccgt gtcagatctg gcagtacggt
    11821 gttatgcgct gtttccgggg cgtattccgg ggaaagtaaa tgcaggatat cagggggagt
    11881 cttttcgaca ggccagatag tttcatcttc gccagtggca tccagtacat tgagaatagc
    11941 gtcaatgctt aacccaccag tgaccatatc ctgcaatgtc tcggcttcgg tgaccaggtc
    12001 acgcaatgaa tccagatcct gcgaggaggg ctcttcgtta aaaggaaaag gatcagcatg
    12061 gcgattaaga tcttgttcgg gagataacaa cgcctcgcgg tattcctgat aaagtcgggt
    12121 cagggcattt cctttttccg caccttctcc ggtggaggag gctccatgaa caggaatgat
    12181 atcgaatgga ttttgtgatg tcagatgctg ctgcctgaac caatccagat ccagatattc
    12241 ggttattgct tccgacgtga tggcagtctg cgatatcgtt tttactgtgc tcaggttcgg
    12301 agatgatgga ttgccatgta caaggcacta tgaagataac ccccattcta tcaggtcgcc
    12361 ttcattcaga cgcatacggt gatgaggttc cacaatctgg tcgtttaccg tacagcaata
    12421 ttcggtagac tgattgataa tccaccatgc ttcttcgtgg cgggtcagag tgagggcaat
    12481 ctttacatca tcattcacct taaaaacagg aatccaggga gaggtaacgg tgaagataat
    12541 ttgtgttcct tcagtcgcgt aaattgtgtt atcggcgatc ccgtgcgcct gtattttttg
    12601 gagtccccac gtaatatttg
//
LOCUS       DAJVPT010000102        12533 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVPT010000102 DAJVPT010000000
VERSION     DAJVPT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OQM47_003095"
     CDS             complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OQM47_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1481152.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OQM47_003096"
     CDS             complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OQM47_003096"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1481153.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2165..3199
                     /gene="rhaT"
                     /locus_tag="OQM47_003097"
                     /pseudo
     CDS             2165..3199
                     /gene="rhaT"
                     /locus_tag="OQM47_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OQM47_003098"
     CDS             complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OQM47_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1481154.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OQM47_003099"
     CDS             complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OQM47_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1481155.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5242..6711
                     /gene="rhaB"
                     /locus_tag="OQM47_003100"
     CDS             5242..6711
                     /gene="rhaB"
                     /locus_tag="OQM47_003100"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1481156.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6708..7926
                     /gene="rhaA"
                     /locus_tag="OQM47_003101"
                     /pseudo
     CDS             6708..7926
                     /gene="rhaA"
                     /locus_tag="OQM47_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8168..8992
                     /gene="rhaD"
                     /locus_tag="OQM47_003102"
                     /pseudo
     CDS             8168..8992
                     /gene="rhaD"
                     /locus_tag="OQM47_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9002..9316
                     /gene="rhaM"
                     /locus_tag="OQM47_003103"
     CDS             9002..9316
                     /gene="rhaM"
                     /locus_tag="OQM47_003103"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1481157.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9617..10063
                     /locus_tag="OQM47_003104"
     CDS             9617..10063
                     /locus_tag="OQM47_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1481158.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10074..11525
                     /locus_tag="OQM47_003105"
     CDS             10074..11525
                     /locus_tag="OQM47_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1481159.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11515..>12533
                     /locus_tag="OQM47_003106"
     CDS             11515..>12533
                     /locus_tag="OQM47_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1481160.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTT"
BASE COUNT         2990 a         3262 c         3310 g         2971 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
     1081 gacattcatt taatcaataa aagtgatcaa aatcacatca attgaaacgc tgttttattt
     1141 ttataggacc attgttttta ttttggaatt gatcacaaaa aaaccaccgc cgttggcgat
     1201 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat
     1261 tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct
     1321 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
     1381 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
     1441 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
     1501 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
     1561 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
     1621 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
     1681 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
     1741 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
     1801 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
     1861 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
     1921 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
     1981 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
     2041 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
     2101 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
     2161 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
     2221 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
     2281 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
     2341 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
     2401 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat
     2461 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
     2521 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact
     2581 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
     2641 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
     2701 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
     2761 tgaagccgct gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt
     2821 catcatgggc ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt
     2881 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
     2941 gttactctcg gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg
     3001 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
     3061 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg
     3121 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg
     3181 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
     3241 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
     3301 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
     3361 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
     3421 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
     3481 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
     3541 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
     3601 agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac
     3661 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
     3721 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
     3781 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
     3841 aatataaaag agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc
     3901 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc
     3961 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
     4021 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
     4081 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc
     4141 cctggcgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
     4201 tactgtcgct gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta
     4261 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg
     4321 tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg
     4381 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
     4441 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
     4501 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
     4561 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag
     4621 acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
     4681 atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca
     4741 gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc
     4801 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
     4861 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag
     4921 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
     4981 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa
     5041 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
     5101 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
     5161 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
     5221 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
     5281 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
     5341 gaaatccatc gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg
     5401 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
     5461 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
     5521 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag
     5581 gcacaacagc aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc
     5641 ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac
     5701 attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac
     5761 tgggaatata ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac
     5821 gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg
     5881 ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
     5941 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
     6001 tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
     6061 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
     6121 ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac
     6181 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat
     6241 cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt
     6301 cgggaaacgg cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac
     6361 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
     6421 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
     6481 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
     6541 atcggcatcg agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
     6601 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
     6661 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
     6721 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
     6781 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
     6841 tttccggttt tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt
     6901 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
     6961 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
     7021 tctcgcgcga ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
     7081 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
     7141 ttacgctctc gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
     7201 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
     7261 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
     7321 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
     7381 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
     7441 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
     7501 cgactgaagt aatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc
     7561 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
     7621 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
     7681 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg cgttgctgga acctaccgct
     7741 gagctgcgca agctggaagc ggcgggcgat tacactgcgc gtctggcact gctggaagag
     7801 cagaaatcgt tgccgcggca ggcggtctgg gaaatgtatt gccaacatca cgatacgcca
     7861 gcaggtagcg aatggctgga gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt
     7921 gggtaaacac tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gaatgtccat
     7981 gcgggctgtc accctcaccc cggccctctc cctgaaaggg cgagggagaa aaacatagcc
     8041 tcgtgcggca acggagctga acaccgctcc ggacagtccc ctcgccccta cggggagagg
     8101 gttagggtga ggggaacaag ccgcaccatg tctaaacaag gcaacctaat ttacaggaac
     8161 acagaacatg caaaacatta cccagtcctg gtttgtccag ggaatgatca aagccaccac
     8221 cgatgcctag ctgaaaggct gggacgagcg caacggcggc aacctgacgc tgcgcctgga
     8281 tgacgccgat atcgcgcttt atcacgacaa cttccaccca caaccgcgct atattccgct
     8341 cagccaaccc atgcctttac tggcaaatac gccgtttatt gtcaccggct ccggcaaatt
     8401 cttccgtaac gtccagctcg atcctgcagc taacttaggc gtcgtaaaag tcgacagcga
     8461 tggcgcgggc taccatattc tctgggggtt aaccaacgaa gccgttccaa cttctgaact
     8521 tccggctcac tttctctccc actgcgagcg cattaaagcc accaacggta aagatcgggt
     8581 gatcatgcac tgccacgcca ccaacctgat cgccctcacc tatgtactgg aaaacgacac
     8641 cgcggtcttt actcgccaac tgtgggaagg cagcaccgaa tgtctggtag tattcccgga
     8701 tggcgttggc attctgccgt ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc
     8761 acaagagatg caaaaacatt cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg
     8821 accgacgctg gatgaagcct ttggtttaat cgacaccgca gaaaaatcag cacaagtatt
     8881 agtgaaggtt tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc
     8941 gctcggcaag cgcttcggcg ttacaccact agccagtgcg ctggcgctgt aaggagcaaa
     9001 catgatccgc aaagcctttg tcatgcaggt aaaccccgac gcccacgaag agtatcagcg
     9061 tcggcataat cccatctggc cggaactgga agcagtgttg aaatctcacg gtgtgcataa
     9121 ctacgccatt tacctcgaca aagcgcgtaa tctgctgttt gccatggtag agattgaatc
     9181 tgaagaacgg tggaatgcgg tagccagtac tgatgtttgc caacgttggt ggaaatatat
     9241 gaccgatgtt atgcccgcta acccagataa cagcccggtg agtagcgagc tgcaagaagt
     9301 gttttacctg ccgtaattgc ccaatgtgcc ggagatgcct ccggcacaat attttaaatt
     9361 tcgatttgaa aaattaaaca tattacatct cccggtaagt tatatttccc tgatacattg
     9421 tgagtaaatc acaaaaataa tgaataaccc attaatgatt catgtggttt atttaaataa
     9481 ccattatgtg cattactccg caaatctgac ctttcactct gtctcaaatt gtcattgttt
     9541 accgacatat cgcctctcat cacttaatcg ctccaccatt acaattgatt aaaatatatt
     9601 ttatggagtc cgattaatgg cagctcttac tgcaagctgt attgacctga atattcaggg
     9661 caatggcgct tattccgttc tgaagcagtt ggcgacaata gcgttacaaa acggttttat
     9721 caccgactca caccagttcc tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg
     9781 atttggttcc ggtgtcgcag tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt
     9841 attattcgcc cgcaaagcgc aggctattga ctggaaagcc agcgatggcg aagacgtcaa
     9901 ttgctggatc tgcctcggcg tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg
     9961 cacactgtgt cgcaaaatta ttcaccagga atttattcat caactgcaac agggcgatac
    10021 cgaccaggtg ctggccttgt taaatcaaac cctcagctca taaggaagtg gcgatggagt
    10081 catccttacg tattgtcgcg atcaccaact gccccgccgg gatcgctcac acctacatgg
    10141 tggcggaagc cctggaacag aaagcccgtt ctctcggcca taccataaaa gtggaaactc
    10201 aagggtccag tggcgttgaa aaccgcttat ccagcgaaga gattgccgct gccgattacg
    10261 tcattcttgc taccgggcgt ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga
    10321 aagtttatga gattgccatc tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat
    10381 taccgacaaa ctcgcagctt tttgccgcag atagcggcgt gaagcttggc aaacaggaag
    10441 tgcaaagcgg cagcgtgatg agccacctga tggcgggcgt ctctgcggcg ctgccgtttg
    10501 tcatcggcgg tggtatcctg gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt
    10561 ataccgatat gtcgaaaggc gcgccgtcgt ttacctgggt agttgaatcc atcggttacc
    10621 ttggcttcac ctttatgatc cccatcatgg gcgcttatat tgcctcgtcg attgccgata
    10681 aaccggcttt cgctccggcg tttctggttt gctatctggc gaacgacaaa gcgctgctcg
    10741 gcacccagtc gggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    10801 tcgtcttctg gttccgcaaa gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa
    10861 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcgggc
    10921 cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt cctccatcaa
    10981 tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    11041 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    11101 acgccatcgt cggcgttgtc gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca
    11161 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    11221 gtgccacagt cgcaaccgaa ccggctatac cttacgctct ggcagctccg ttgccgatga
    11281 ttaccgccaa tacgctggcg ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca
    11341 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtag
    11401 gttcgtttta tctggtgctg gcgattggcc tcgcgctgaa catttcattc attatcgtcc
    11461 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    11521 attgagttac tgcaacagtt gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt
    11581 cgcgacatcc tgataaacac gctggaacct tgcgttaatg aaatcacctt tgatggtctg
    11641 ggcagctttg ttgcccgtaa gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg
    11701 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg tttcaccacc
    11761 attggtggct ggtggaatca gtcgatgctc aaccatcggg taaccatacg cacacacaag
    11821 ggagtgaaaa tccctggtgt gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa
    11881 aggcaacagc cgctgtcatt tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat
    11941 gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc caattttgcc
    12001 tgctggggcg aagataaagt ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg
    12061 atggctgaac tattgcagac ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc
    12121 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    12181 gtagtgatcg tgctggatac cgctgtcgca ggcgatgttc cgggcattga taacattaaa
    12241 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    12301 aaccagaaac tggtagcagc gttaaaaaac tgtgccgcac ataacgattt accgctgcaa
    12361 ttttccacca tgaaaaccgg agcgacggat ggcgggcgct acaacgtgat gggcggcggg
    12421 cgtccggttg ttgcgctgtg cctgccaacc cgctatctgc acgctaacag cggcatgatt
    12481 tcaaaagccg attacgatgc tctgctcacg ttgatacggg gttttctgac gac
//
LOCUS       DAJVPT010000103        12373 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVPT010000103 DAJVPT010000000
VERSION     DAJVPT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            181..2028
                     /gene="ldtD"
                     /locus_tag="OQM47_003107"
     CDS             181..2028
                     /gene="ldtD"
                     /locus_tag="OQM47_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1481161.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2209..2757
                     /locus_tag="OQM47_003108"
     CDS             2209..2757
                     /locus_tag="OQM47_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1481162.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2784..3431
                     /gene="gloC"
                     /locus_tag="OQM47_003109"
     CDS             2784..3431
                     /gene="gloC"
                     /locus_tag="OQM47_003109"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1481163.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OQM47_003110"
     CDS             complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OQM47_003110"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1481164.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OQM47_003111"
     CDS             complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OQM47_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1481165.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OQM47_003112"
     CDS             complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OQM47_003112"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1481166.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OQM47_003113"
     CDS             complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OQM47_003113"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1481167.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9755..12370
                     /gene="pepN"
                     /locus_tag="OQM47_003114"
     CDS             9755..12370
                     /gene="pepN"
                     /locus_tag="OQM47_003114"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1481168.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
BASE COUNT         3157 a         3071 c         3149 g         2996 t
ORIGIN      
        1 cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga
       61 agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac
      121 aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg
      181 atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta
      241 acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc
      301 ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg
      361 gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct
      421 caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca
      481 cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg
      541 gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga gttactgacc
      601 gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat gatgggctat
      661 ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag cagtaaacct
      721 tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc gctggataaa
      781 ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg
      841 catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact gaccggcaaa
      901 gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa
      961 cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact
     1021 gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac
     1081 aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta cgataatgaa
     1141 ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga tggtgctatt
     1201 ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggca
     1261 ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac
     1321 attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga ttcgcgagtc
     1381 attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa caacgtagtg
     1441 gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct gccgaaagtg
     1501 cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc
     1561 agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc gaatttaccg
     1621 tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg
     1681 ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat
     1741 acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg
     1801 ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat
     1861 acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt
     1921 gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg
     1981 cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc
     2041 ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc
     2101 actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc
     2161 gccagaagtg catataaacg ataacattga gctgtagact tgattatcat ggacaaattc
     2221 gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg
     2281 ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat
     2341 cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa
     2401 gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc
     2461 gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg
     2521 gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg cgcccgcagc
     2581 cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa
     2641 ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga
     2701 tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa
     2761 tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc
     2821 agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc gatcctggcg
     2881 gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc
     2941 tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg
     3001 gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc
     3061 aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg
     3121 aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata
     3181 cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta
     3241 ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt
     3301 cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg
     3361 gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa gacgaaatgc
     3421 ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat ttctggcaaa
     3481 gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca aaatcgtgca
     3541 aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca ggcaatgttg
     3601 cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa cgcttacagc
     3661 actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc ggccacattt
     3721 acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag cacttgttct
     3781 tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct gaagtcgcgg
     3841 tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg aatacgctgg
     3901 cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct caggatggtg
     3961 gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat cgccgctttc
     4021 atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt acaagcgcca
     4081 acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat cagctcttta
     4141 tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg ggcaaaaccc
     4201 tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg tgccagtgtc
     4261 tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc atggaacagc
     4321 actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa gtcaagggtg
     4381 tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga gttaaagacg
     4441 ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct ggtatttttt
     4501 gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg tgccgtgcga
     4561 gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg agtgcagcga
     4621 ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt ttcgagcaga
     4681 tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc atctttatag
     4741 acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag atcggccagg
     4801 cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag gttccattat
     4861 ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt ccaactatta
     4921 ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg cataaaaaaa
     4981 acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt agaactggta
     5041 aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt cagaatcgat
     5101 ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt aggttgcgcc
     5161 cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt tcgctttaga
     5221 tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg caactaacag
     5281 aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag tgatcggcgt
     5341 agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt cgtacttcag
     5401 accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt cttgcaggtt
     5461 ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt cgtagctgat
     5521 agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt ttttacccag
     5581 gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt tacgataggt
     5641 agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat caccaccaaa
     5701 ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt tacggccgta
     5761 atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg ttttgttacc
     5821 agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc actgaccata
     5881 accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg cataggtcat
     5941 gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat gcagaccaac
     6001 agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg cagcgtttgc
     6061 agtacctgct actaacagag cagggacgat cactgccaga atattgcgct tcatcattat
     6121 ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata
     6181 aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc
     6241 attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa
     6301 agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg tcatatttcc
     6361 atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta cttatattta
     6421 aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct
     6481 ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa
     6541 actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa tgtaatattc
     6601 cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta acaaacagat
     6661 aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca
     6721 gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc
     6781 agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc
     6841 gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat
     6901 acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat
     6961 gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata
     7021 gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc
     7081 cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa
     7141 cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc
     7201 ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa
     7261 tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc
     7321 agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa
     7381 ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata
     7441 aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa
     7501 agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag
     7561 gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc
     7621 ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc
     7681 ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag
     7741 gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc
     7801 tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc
     7861 ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg
     7921 caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa
     7981 gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg
     8041 tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc
     8101 tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc
     8161 cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt
     8221 cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat tagctccctg
     8281 atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac
     8341 aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt
     8401 accgttacac tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca
     8461 ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag
     8521 ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc
     8581 aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga
     8641 gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac gcaggaaagc
     8701 atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact cttccagcca
     8761 ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg
     8821 gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta
     8881 gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac
     8941 ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag
     9001 accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc
     9061 aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg
     9121 aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt
     9181 atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata
     9241 gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag
     9301 gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc
     9361 acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg
     9421 ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa
     9481 ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt
     9541 caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc
     9601 cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt
     9661 ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa
     9721 caccagactc tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata
     9781 ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct
     9841 cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga
     9901 tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga
     9961 gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta atttgccgga
    10021 gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg cgctggaagg
    10081 gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt tccgccatat
    10141 tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa ttattgccga
    10201 taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag gcgaactcga
    10261 aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct acctgtttgc
    10321 gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt ctggtcgcga
    10381 agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt gggcgatgac
    10441 ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt atgacctcga
    10501 catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga ataagggtct
    10561 gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg acaaagatta
    10621 cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg gtaaccgagt
    10681 gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct tccgcgatca
    10741 ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg tgcgcaccat
    10801 gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc gcccggacat
    10861 ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg cggaagtgat
    10921 ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc aactctattt
    10981 cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga tggaagatgc
    11041 gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta cgccggttgt
    11101 gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca tcagccagcg
    11161 cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt ttgccatcga
    11221 attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc cggtgaattc
    11281 cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct acttccagcc
    11341 ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata agtggagcga
    11401 tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt gggatgcggc
    11461 gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc aagggcagcc
    11521 gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg atgagaagat
    11581 tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa tggctgaact
    11641 gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca ctcgtactct
    11701 ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc agagcgagta
    11761 ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc tgcgcttcct
    11821 tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc atgaagcaaa
    11881 caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc tgccttgccg
    11941 tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg tgatggataa
    12001 atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg tgcgtggtct
    12061 gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt tgattggtgc
    12121 gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt accagttcct
    12181 ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac gtctgattga
    12241 accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc gcgcggcgct
    12301 ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga taactaaagc
    12361 actggcttga tac
//
LOCUS       DAJVPT010000104        12296 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVPT010000104 DAJVPT010000000
VERSION     DAJVPT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..399
                     /locus_tag="OQM47_003115"
                     /pseudo
     CDS             1..399
                     /locus_tag="OQM47_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            482..739
                     /locus_tag="OQM47_003116"
     CDS             482..739
                     /locus_tag="OQM47_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481169.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1297..2064)
                     /gene="fecE"
                     /locus_tag="OQM47_003117"
     CDS             complement(1297..2064)
                     /gene="fecE"
                     /locus_tag="OQM47_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1481170.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2065..3021)
                     /gene="fecD"
                     /locus_tag="OQM47_003118"
     CDS             complement(2065..3021)
                     /gene="fecD"
                     /locus_tag="OQM47_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1481171.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3018..4016)
                     /gene="fecC"
                     /locus_tag="OQM47_003119"
     CDS             complement(3018..4016)
                     /gene="fecC"
                     /locus_tag="OQM47_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1481172.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4013..4915)
                     /gene="fecB"
                     /locus_tag="OQM47_003120"
     CDS             complement(4013..4915)
                     /gene="fecB"
                     /locus_tag="OQM47_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1481173.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4960..7284)
                     /gene="fecA"
                     /locus_tag="OQM47_003121"
     CDS             complement(4960..7284)
                     /gene="fecA"
                     /locus_tag="OQM47_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1481174.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7371..8324)
                     /gene="fecR"
                     /locus_tag="OQM47_003122"
     CDS             complement(7371..8324)
                     /gene="fecR"
                     /locus_tag="OQM47_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1481175.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8321..8842)
                     /gene="fecI"
                     /locus_tag="OQM47_003123"
     CDS             complement(8321..8842)
                     /gene="fecI"
                     /locus_tag="OQM47_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1481176.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9140..9322
                     /locus_tag="OQM47_003124"
                     /pseudo
     CDS             9140..9322
                     /locus_tag="OQM47_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9316..9420
                     /locus_tag="OQM47_003125"
                     /pseudo
     CDS             9316..9420
                     /locus_tag="OQM47_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9759..10016
                     /locus_tag="OQM47_003126"
     CDS             9759..10016
                     /locus_tag="OQM47_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS1481177.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(10336..10467)
                     /locus_tag="OQM47_003127"
                     /pseudo
     CDS             complement(10336..10467)
                     /locus_tag="OQM47_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10749..12107
                     /locus_tag="OQM47_003128"
     CDS             10749..12107
                     /locus_tag="OQM47_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1481178.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         2921 a         3229 c         3360 g         2786 t
ORIGIN      
        1 gttctcgccg gacagcaggc ttaccatgaa agcactggaa atggcatggg aaacccgtgg
       61 taagcccgtc ggggtgatgt tccaagcgat caaggcagtc attatacgag caggcagttc
      121 cggcagttac tgtggcgata ccagatcaga cagagtatga gccggcgtgg aaactgctgg
      181 gataacagcc caatggagcg cttcttcagg agtctgaaga actaatgggt gccagcgacg
      241 ggctatgtaa gcttcagcga tgcagctcac gcaataacgg actatatcgt tggatattac
      301 agcgcactaa gaccgcacga atataatggt gggttaccac caaacgaatc agaaaaccga
      361 tactggaaaa actctaacgc ggaggccagt tttagttgac cacaacagac tacctgaagg
      421 gagccgcggt cgcctagcag ttgcagtagc aggagatcat ccagccgcag tacaggtcac
      481 gatgactctg gttaatgata ccggctttga ccccgtattt tccggctcta tcgctgaatc
      541 atggcgtcag cagccgtgca caccatccta ttgttgtgac tgggaggctg ccaccatgct
      601 tcgcgctttc cctctggcga aaaagggaga aggacgggcc cgtctgcctt cactttatgc
      661 cagcttcggt aagctgggtg agacaccgac tcatgaagat atcattgata acaatcgatc
      721 catcaactgg cctgtataac gtggctgccg gtgattaaga aagctgcacc tacctaagta
      781 gtagcaaacg cacacttttt agaaaaatcg atggtcagaa actggattag caattccgtt
      841 ccagggttgc ttttgattta cgttggcgtc tgatcattga tttatcctca aaagcccaac
      901 ctcattggta atgaaccagc tccgtgaatg tccgctctgg cacagagcga aattttttga
      961 tctccccccc tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac
     1021 ttcgcgtttt tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat
     1081 tgattgccgt gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg
     1141 cgttgattat gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt
     1201 tcagagtccg gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc
     1261 attcagtcgt ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct
     1321 gccagatacc ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc
     1381 tggggtcatc acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac
     1441 cagttgatcg cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt
     1501 cccctgagtc cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag
     1561 ataggtggtt ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccatcgccag
     1621 aaatgcgcgc tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt
     1681 gatccgggtc tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc
     1741 ccagagtgac agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc
     1801 tggcgttaaa tggtgctgag gcagcagcga aagcctgcgg gccaactggc gtgatgagag
     1861 catatttatg ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga
     1921 aaaacagttt aacagcgtcg atttcccgca accgttaggg ccgatcaggg cggtgatctt
     1981 ccccgttggc agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aactgaccgt
     2041 cagattttca gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg
     2101 cgcaccgata atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct
     2161 cgccagcaga tcggcaacca ccaacagcaa cgcacctgtc agggctgaaa caggcagcag
     2221 tctgcggtga cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa
     2281 gctaatcggg ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc
     2341 ccagaatcgg gtatggggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc
     2401 aaggaggtcg agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg
     2461 gatggcaatc ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag
     2521 ggcgttgttc acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca
     2581 tgcagaaagc gccacgccgg tgagcgccag cttcatcggc tggtgggtct ttgccagcat
     2641 cttcagtaat atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac
     2701 gggcagtgac ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac
     2761 gccgagaata tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc
     2821 cacggcgagg gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat
     2881 caatacataa taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac
     2941 ggggatcact cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa
     3001 aataaccagc gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca
     3061 atcagcgcca gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt
     3121 acatctgcca gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc
     3181 tgatcgaagc ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc
     3241 ggacctgcca cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa
     3301 cgtagcctcg tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg
     3361 ttcagttgat tcgccagcag taacacgaca gggactgcag tgaccaccac cggcaagagc
     3421 tgccagacat cctgccagcg ggcgtgggac actccgcctg ccagccagta aaagatgccg
     3481 taagcatgat cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac
     3541 agcgcgatac ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct
     3601 gcggtcatga ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa
     3661 tagcctgcaa tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg
     3721 ctgttaatgc cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc
     3781 gtgcccgcga gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga
     3841 aggttttgca ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt
     3901 gcatctgctc cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata
     3961 ataagtgctg caacgggaag cccccacagc agcatcgggt gttttatcgc ggtcatttca
     4021 caacggtaag cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag
     4081 caaaaatacc gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct
     4141 gcgcggcggt taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt
     4201 cgcgatagtg ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga
     4261 tggacggcat ggacgaaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca
     4321 cgctgccggt ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa
     4381 aggccacgcg tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt
     4441 gttccagacg tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag
     4501 attgcaaatt ttcagcgtag gtttcgttgc gggacttaag cagcagtacc ggcgcgattt
     4561 gctgcaaggc gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt
     4621 tcagagcggc aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt
     4681 tcaggtgcgc acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga
     4741 tcgggctgac gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc
     4801 gttgtggcgt tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccatg gcggcaaagg
     4861 cgtggctgat caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca
     4921 cattccagct aaaagcccgg caagccgggc gttaacacat cagaacttca acgacccctg
     4981 catatacagc gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg
     5041 gatgaagtag tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat
     5101 ctgcgggcca aagtcatacg ccacgcgtgc accccagagc atgaagccgg gaatgcggcc
     5161 ggtactgccg tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg
     5221 gaaatcgctg ttcagattga acgtccagtt tcctggcttg tagtccacgc ccagcgtgcc
     5281 tttatgtttc ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc
     5341 cgcgttcaca tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt
     5401 acccagatcg taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt
     5461 gacggtgtcg ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc
     5521 catttccgcc gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt
     5581 ttccggttcc acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc
     5641 gaacgagcct tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt
     5701 caacgccgga agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt
     5761 ctggtatgac tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc
     5821 gattttgtca tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta
     5881 agggcttgag ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg
     5941 cgttgattca ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat
     6001 aaagatctgg ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt
     6061 gatgcgtttg ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat
     6121 gttgaattta tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg
     6181 accccagaag cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg
     6241 agacaggcca ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt
     6301 gtgcacctca tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct
     6361 gtgctcgcgc cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt
     6421 gtccgctgtg ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt
     6481 tgggctgagc tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt
     6541 aacaaagttc accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg
     6601 taccacgtca atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg
     6661 accgtaaggg gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag
     6721 gcgcgggttc aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt
     6781 gttttccggc gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc
     6841 ggttttggcg aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc
     6901 gttttcacgc gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc
     6961 gggcgcgggc tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc
     7021 gtccagcagt tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg
     7081 cttgccgcgc gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg
     7141 attgagcgct ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa
     7201 cgagagaccg gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt
     7261 acgaaaaacg cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat
     7321 gagtcgaacg agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt
     7381 gaaatgttta tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata
     7441 acgttcagga tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca
     7501 acggcgggat cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca
     7561 cccagcggtt tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc
     7621 agcggtttca ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg
     7681 atgcgttttt gcgcgggggc actggcgaga agcacttcca cagcgtgctg ctgaacgtca
     7741 agctgcgtga aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg
     7801 ccctgacggg tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg
     7861 atttcaccgt accagagccg gacggtgcgc tgatgcgcat caaaacgcac atccgccgcg
     7921 ctttgggtat tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct
     7981 ttggcggtgc ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag
     8041 cctccgccag cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg
     8101 gtatcgtgca acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg
     8161 ttttcaacct gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag
     8221 cgcgcttcct gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct
     8281 gaacgcagcg cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac
     8341 ggaacagcag gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa
     8401 caccgagttt gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg
     8461 cttcacgtgt tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga
     8521 gttggctttc gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct
     8581 ccagatacgc tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga
     8641 tagtgcagag gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc
     8701 gcaaaaaagt gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca
     8761 gccagctttt caaccagccg tgatgtgtgc cataaagcga ctcgaacgtt aaggaagctg
     8821 tggtagtggc gcggtcagac atgcggagtg catcaaaagt taattatcac gtagtcatat
     8881 taatatgaga atggttatca ttacaattgg aaataaaatt gtttccaata gacattttta
     8941 acatgttgtt tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct
     9001 gacatgtgtc ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc
     9061 ctaaaggatg gggatttcgg taatgatctt acccagcaat agtggacacg cggctaagtg
     9121 agtaaactct cagtcagagg tgactcacaa gacaaaacag tttcaaccag taaaaaaacg
     9181 taaacaccat tcgcatgaat ttcgtagtta agatctgaag cttgcagaat gcatcggtgt
     9241 tactgccgga gtccgtgaac ttagtctgca tgaatcacca cagctttaca tctgtcgcag
     9301 taaacagcaa aatcagcaaa cttacttaat tatatcgagt gcgattacaa tcgctggcga
     9361 tggtacagtt ggtgtggcgg tctcagtccg gaacaatttg aaaatcataa tctcacttaa
     9421 aacggcatcc attttacaaa agatggccgt ctgtgtattt gtaatgaata ccttctacat
     9481 gtttagagtg cataatccac gacaaggcga tatcctaaaa attagctagc aggcaacttt
     9541 gatggcatag ataatgtttt tagtaaaaaa gagttgactg aatataatac agcctcttcc
     9601 tacataagaa ggaatgtgct cacaaaaaaa accattagtc tgcttcctgt tcttacaata
     9661 atcagtatta tgtattttat ttatatagca aaatatgctt gttacacctg tcataattag
     9721 gcatggtaaa attattgact gaaccctggg ggagaaagat gcacagtgat tattataata
     9781 tggtttttgg tgaaaaattg gccaatattt tatatgaagc taactctcaa tttttttacg
     9841 agagaaatgt catagaagag gcagtcaacg ctttattctg tgagcgtgaa ataataaaca
     9901 acaagaacat aataaagaag ctgatgttct ttttgtcaga tgttaatcac accaagaaag
     9961 atgttgttca gagcgcactt aatattatca ttgacattac atcaggtgat atttgagatt
    10021 ttacataaaa gataatctct caaggagtct tatctgaaaa cgaaatatat agtccctctc
    10081 gttttgtaat atcgattggc tttgttgaat cggttacgta ccccggagcc ggtcatattt
    10141 tcaggtaata cacacgcttt cagacatttc ggctttcgtc atttttgtat catgggcata
    10201 gcttctgcta cctgaccatc ataatcacgc agcttcagtg aagcagtgac cacctgttta
    10261 acccggtgta tcgttatgat cttatccagc aattgaaccg ccccgggttt cctggagagt
    10321 gttttatctg tgaactcagg ctgccagatc atcgtttccg atggaagcat aataagcttt
    10381 ttctgcttct gccggtggga tatggccctg cctttccagc aatcgtcgat tgttatacca
    10441 gtccacccac gtgagtgtgg tcagctcatc atactgacac gtgcattacc cctaccgcga
    10501 taatgcaacc gtacaatata gggcgtttcc taaacgcagc cctcatcact ccaatccaac
    10561 aagacacagc atttttgatc acgaagaaag acaaataatt tccgtaaatg tttccgttgt
    10621 tacaaataac aacagtgttt tacatttctt tggcctcagg cccgcgcatg ttgccattcg
    10681 atgtcatttt cctgactcgt ttcttcttta tggtgtagtg tgatcttcac tttatagatg
    10741 ctaacaatat gaaaagaaaa attattcccg tgctaatcgg ttgtacgctc tctttctctg
    10801 ccctggcggc acaacctacg gctgagcgct atgtcgtcag ttttccagaa ggcacgcacg
    10861 tgaactacgc cggcgcattt gccagtgcat tcccgaacgg ccttccggtt gggataggtt
    10921 ctggcctgct gttcaccggt aaacagggcg atgccctgac attcgcaacc attaccgatc
    10981 gcggtccgaa cgcggattca ccgaaagagg ggaaaaacga aactaaaatt ttcgttacgc
    11041 cagatttcgc gccgctgctg atgacgattc gtgtacaaaa cggcaaagcg gaggccatcg
    11101 atccccgtcc tctgcatgac gataaaggcg cgatcaacgg tctgccgctg gcaagcgacg
    11161 tgattggctc caccaatgaa gtggccttca gcgacacgtt acatagactg aagggggata
    11221 atcgcgggct ggatacggaa gggatcactc cggatggtaa aggaggttac tggctgtgcg
    11281 atgagtatgg cccgttcctg attaacatcg acagtaaagg gaaaatcctg gcaatccacg
    11341 gtccgcaggc ggcggaaggg gaaaaagcga ttgcgggcgg tttgccaaat atcctcaaat
    11401 ggcgtcaggc gaaccgcggc tttgaaggcc tcacccgtat gccggacggg cgtattattg
    11461 tcgccgtgca aagtacgctg gatatcgacg ctaagagcaa gaaaaaggcg ctgtttacgc
    11521 gtctggtgag cttcgatccg gcaagcggaa aaaccgcgat gtacggttat cctatcgaca
    11581 gcgcggccta cagcaaaaac agcgacgcta aaatcggaga catcgtggcg ctcgataacc
    11641 agcacatcct gctgatcgaa cagggaagag ataaaaacaa cagaatgcgt aacctgattt
    11701 acgaggtcga tctcaataag gcgagcgacc tgtcaggctt cgacaagccg ggtgagtatc
    11761 cggagtttga tgatgagaaa accctgtcac agcgcggcat tacccttgcg cagaaaacgc
    11821 aggttgttga tctgcgctcc ttgggctggc agcaggagaa ggctgaaggg ttggcgctga
    11881 tcgacagcaa aacgctggcg gtggcgaacg ataacgactt tggcgtgaag gtggcgatgc
    11941 aacatccggt cgagggcaag acattcaagg attatcgggt gaacgcggaa ggaaagctga
    12001 cgcttgacga caagcaggtt gaaacgaccc tcagagtgaa gccactggag aaaccggagt
    12061 ctgacagtga actgtggatt gtgacgctgc cggaagcgtt gaaataagtt tataaggtac
    12121 ggacagcagt atcgcttagt ttttcaaatt catgtatgtg aacaatattg gggttccggc
    12181 gtagaccccc aatgaaagcg tcaccacgtg aaaacatgcc tgcacggttt acacgtcatc
    12241 ccatggtggc agccggttaa ctttcctgcg ccaccagtgg aaaaaggcca taagca
//
LOCUS       DAJVPT010000105        12282 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVPT010000105 DAJVPT010000000
VERSION     DAJVPT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="OQM47_003129"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="OQM47_003129"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1481179.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="OQM47_003130"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="OQM47_003130"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1481180.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="OQM47_003131"
     CDS             3116..3400
                     /locus_tag="OQM47_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1481181.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OQM47_003132"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OQM47_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1481182.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OQM47_003133"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OQM47_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1481183.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OQM47_003134"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OQM47_003134"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1481184.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="OQM47_003135"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="OQM47_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1481185.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9586..10686
                     /gene="ompC"
                     /locus_tag="OQM47_003136"
     CDS             9586..10686
                     /gene="ompC"
                     /locus_tag="OQM47_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1481186.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFIRAVDIRTDNIVATGITYQF"
     gene            10850..11335
                     /locus_tag="OQM47_003137"
     CDS             10850..11335
                     /locus_tag="OQM47_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1481187.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11445..>12282
                     /locus_tag="OQM47_003138"
     CDS             11445..>12282
                     /locus_tag="OQM47_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1481188.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFL"
BASE COUNT         3200 a         3056 c         2889 g         3137 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgacccc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcattt cgacttcgat cacatatcga taaataatat tattctattt ttctcattgt
     9481 ttttaatcac cattctattt taaaaacagt atgtcattta taaatgacgc cgtttaaaat
     9541 tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata
     9601 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
     9661 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
     9721 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
     9781 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
     9841 gatgaagcca acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt
     9901 agtgaatttg gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca
     9961 tggaccgata tgttgccaga atttggtggc gatacctgga gtcagaaaga tgtcttcatg
    10021 acataccgta ccaccggtct agcaacctat cgcaactacg atttctttgg cttaattgaa
    10081 gggctgaact ttgccgcgca atatcaaggc aaaaataaac gtactgacaa cagtcatctt
    10141 tatggtgctg acgacacgcg tgccaacggt gacggtttcg gtatctcctc aacttatgtt
    10201 tatgatggct ttggtatcgg tgcggtatat accaaatccg atcggacaaa tgcgcaggaa
    10261 agagccgcag ctaatcctct caatgcctcc ggtaagaatg cagaactatg ggccacaggt
    10321 ataaagtatg atgccaacaa catctacttt gcagctaatt acgatgaaac attaaacatg
    10381 accacctatg gggatggtta tatctctaac aaagcacaaa gttttgaagt ggtggcgcaa
    10441 tatcaattcg acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa aggcagagat
    10501 ctgggccgct acgccgacca ggacatgatt gagtatatcg acgttggtgc gacgtatttc
    10561 ttcaacaaaa atatgtcgac ctatgttgat tataaaatca acctgattga tgaaagcgac
    10621 tttatccgtg ccgtagatat tcgcaccgat aacatcgtcg ctacgggcat tacctatcag
    10681 ttctaacgtc ctctcctgac cagcccttct ctgggctggt ttttgattta agtgctgctg
    10741 tatgactcct taagtaaaaa tgagcaattc ttgatatttc tggtcattca cctcaattaa
    10801 tattattcca tatcctccgg ggacatctta ctataatatt tattgatgag tgattatgac
    10861 caatattaat acagcttgtg taaaaaataa tgccagttat caacttaata acgcattacc
    10921 taacaaagaa accatctcca gcaatttttg tgaacgacta gaacaatggg gtaataaatc
    10981 ggttaataat ggtgaagaaa gagcaattgc cgtggagcga attaaagaag cttacaattc
    11041 gaacatggca tctcttgatt tgtcatatct tgatttgagt gaattaccgc ctatcccctc
    11101 gacagttaat acgttaaatc tggaaaacaa ttgtctaact tgtcttgact tagctgacaa
    11161 tgccagtctc gttaatatca acctcagcgt taataaaatt aacacgataa cctttccaaa
    11221 agaaccaaaa ctggaaaaca tttatattaa tcataacaat ctggaaagtt tagatttaaa
    11281 aaatcagcat tcattggtta atctggaagc gcaaaataat agcctaaaaa aatgatattt
    11341 ctgatagtta taaactgaaa tttcttaatc ttgattataa caagctagca tcgctggatg
    11401 tctcccggca agaatctctg attgagttaa gtgcccatca caatatgatc actgacctta
    11461 tattacacaa tcatcccagg atgaaaacaa tcactttaaa cgacaaccat attgcacatt
    11521 taaacgccaa aaacactaca aaactggaat atttaaactt aagcaataac aatttactgc
    11581 caaccaatga cattgatcaa ctaatatcat caaaacatct ttggcatgta ttagttaacg
    11641 gcatcaacaa tgatccactt gcacaaatgc agtactggac tgcagtaaga aatataattg
    11701 atgacactaa tgaagtgacc attgatttat caggacttaa tttaaccact caaccaccag
    11761 ggctgcaaaa cttcacctct atcaatcttg ataataacca actcacacat tttgatgcaa
    11821 ccaactacga tagactcgta aagctaagtc tgaatagtaa tgctcttgag tcaataaatt
    11881 ttcctcaagg cagaaatgta agtattacac atatatctat gaataataat gctctcagaa
    11941 atattgatat agataggctt tcatcagtta cttattttag tgcggcacat aatcaactag
    12001 agtttgtgca attagaatct tgcgaatggc tgcaatacct gaatctcagc cataatcaat
    12061 taactgatat tgttgcaggt aataaaaatg aactcttact gctggatcta tcccataata
    12121 aactaacaag tttacacaat gtcttatttc ccaacttgaa tacgttactt attaacaaca
    12181 atttgctttc tgaaattaaa atattctata gcaacttccg caatgttcag acattaaacg
    12241 ctgctaacaa ccagttgaaa tatataaatc ttgatttcct ga
//
LOCUS       DAJVPT010000106        12221 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVPT010000106 DAJVPT010000000
VERSION     DAJVPT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..492)
                     /locus_tag="OQM47_003139"
     CDS             complement(<1..492)
                     /locus_tag="OQM47_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1481189.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OQM47_003140"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OQM47_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1481190.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OQM47_003141"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OQM47_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1481191.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OQM47_003142"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OQM47_003142"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1481192.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OQM47_003143"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OQM47_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1481193.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OQM47_003144"
     CDS             3646..4155
                     /locus_tag="OQM47_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1481194.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OQM47_003145"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OQM47_003145"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1481195.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OQM47_003146"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OQM47_003146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1481196.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OQM47_003147"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OQM47_003147"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1481197.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OQM47_003148"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OQM47_003148"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1481198.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OQM47_003149"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OQM47_003149"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1481199.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OQM47_003150"
     CDS             complement(8797..9690)
                     /locus_tag="OQM47_003150"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1481200.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OQM47_003151"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OQM47_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OQM47_003152"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OQM47_003152"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1481201.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12221
                     /gene="allE"
                     /locus_tag="OQM47_003153"
     CDS             11580..>12221
                     /gene="allE"
                     /locus_tag="OQM47_003153"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1481202.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2967 a         3159 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttataa c
//
LOCUS       DAJVPT010000107        12165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVPT010000107 DAJVPT010000000
VERSION     DAJVPT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1040
                     /locus_tag="OQM47_003154"
     CDS             <1..1040
                     /locus_tag="OQM47_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1481203.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OQM47_003155"
     CDS             1041..3206
                     /locus_tag="OQM47_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCS1481204.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OQM47_003156"
     CDS             3220..4131
                     /locus_tag="OQM47_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCS1481205.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OQM47_003157"
     CDS             4131..5426
                     /locus_tag="OQM47_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCS1481206.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OQM47_003158"
     CDS             5471..5701
                     /locus_tag="OQM47_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481207.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OQM47_003159"
     CDS             5679..6179
                     /locus_tag="OQM47_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCS1481208.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OQM47_003160"
     CDS             6179..7597
                     /locus_tag="OQM47_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCS1481209.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OQM47_003161"
     CDS             7597..8445
                     /locus_tag="OQM47_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481210.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OQM47_003162"
     CDS             8445..8900
                     /locus_tag="OQM47_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCS1481211.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OQM47_003163"
     CDS             8903..9595
                     /locus_tag="OQM47_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1481212.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OQM47_003164"
     CDS             9605..10936
                     /locus_tag="OQM47_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCS1481213.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12165
                     /locus_tag="OQM47_003165"
     CDS             10937..>12165
                     /locus_tag="OQM47_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1481214.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNP"
BASE COUNT         3259 a         3027 c         3207 g         2672 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gaagcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccaga
//
LOCUS       DAJVPT010000108        12095 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVPT010000108 DAJVPT010000000
VERSION     DAJVPT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..314)
                     /locus_tag="OQM47_003166"
     CDS             complement(<1..314)
                     /locus_tag="OQM47_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCS1481215.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVS"
     gene            602..1693
                     /gene="rutA"
                     /locus_tag="OQM47_003167"
     CDS             602..1693
                     /gene="rutA"
                     /locus_tag="OQM47_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1481216.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            1693..2385
                     /gene="rutB"
                     /locus_tag="OQM47_003168"
     CDS             1693..2385
                     /gene="rutB"
                     /locus_tag="OQM47_003168"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1481217.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2397..2783
                     /gene="rutC"
                     /locus_tag="OQM47_003169"
     CDS             2397..2783
                     /gene="rutC"
                     /locus_tag="OQM47_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1481218.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            2791..3591
                     /gene="rutD"
                     /locus_tag="OQM47_003170"
     CDS             2791..3591
                     /gene="rutD"
                     /locus_tag="OQM47_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1481219.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3601..4191
                     /locus_tag="OQM47_003171"
     CDS             3601..4191
                     /locus_tag="OQM47_003171"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1481220.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4202..4696
                     /gene="rutF"
                     /locus_tag="OQM47_003172"
     CDS             4202..4696
                     /gene="rutF"
                     /locus_tag="OQM47_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1481221.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            4717..6045
                     /gene="rutG"
                     /locus_tag="OQM47_003173"
     CDS             4717..6045
                     /gene="rutG"
                     /locus_tag="OQM47_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1481222.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6127..6234)
                     /locus_tag="OQM47_003174"
     CDS             complement(6127..6234)
                     /locus_tag="OQM47_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481223.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6607..7203
                     /gene="wrbA"
                     /locus_tag="OQM47_003175"
     CDS             6607..7203
                     /gene="wrbA"
                     /locus_tag="OQM47_003175"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1481224.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7224..7451
                     /locus_tag="OQM47_003176"
     CDS             7224..7451
                     /locus_tag="OQM47_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1481225.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7489..8730)
                     /gene="agp"
                     /locus_tag="OQM47_003177"
     CDS             complement(7489..8730)
                     /gene="agp"
                     /locus_tag="OQM47_003177"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1481226.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9023..10279)
                     /locus_tag="OQM47_003178"
     CDS             complement(9023..10279)
                     /locus_tag="OQM47_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1481227.1"
                     /translation="MGSNIHGISCTANNYLKHAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10540..11460
                     /gene="cbpA"
                     /locus_tag="OQM47_003179"
     CDS             10540..11460
                     /gene="cbpA"
                     /locus_tag="OQM47_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1481228.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11460..11765
                     /gene="cbpM"
                     /locus_tag="OQM47_003180"
     CDS             11460..11765
                     /gene="cbpM"
                     /locus_tag="OQM47_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1481229.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11858..>12095)
                     /locus_tag="OQM47_003181"
     CDS             complement(11858..>12095)
                     /locus_tag="OQM47_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HCS1481230.1"
                     /translation="PADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWL
                     PEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         2890 a         3066 c         3058 g         3081 t
ORIGIN      
        1 gagacttcca gcttcagacg gatgtactct tttatcgccg ccagcggggc gaaatcttca
       61 cgaaacgctt ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac
      121 agcgcctctt ttgacgggaa gtaatacagc agattggttt ttgaaacacc cgccaactct
      181 gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg
      241 ctaagaatcg cttttttctt cgcgcttact gcgcgcgaac gtttacccgt tgttttcact
      301 gcgccttgcg tcatgcgctc tcccctcttg tagattggta acaggatagc aaaacccccg
      361 cccggcctcg accttctgca ctctcatcgc gcacggatgc atgttttatg tgcaactgtt
      421 ttgaccgttt agtccacttt ttaccagata ttttagataa cacaatgcta acacattaat
      481 aatattcact ttttaaaaac tggcatccgc tttgcgaaca agccaatacg cggctgaaaa
      541 ggatatgcag gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat
      601 gatgaaaatt ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc
      661 gccgcagtac atgccgacct ttgaactgaa taaagccatt gcgcaaaaag cggagcatta
      721 ccatttcgat ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt
      781 ctgggatcac aaccttgagt cgttcacctt gatggcgggt ctggcagccg tgacctcgcg
      841 cattcagatt tacgccactg ctgccacctt aacgttacct ccggcaatcg tcgcccgtat
      901 ggccgcaacc atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg
      961 gcaaaagccc gagtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg
     1021 ttacgactat ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cggggaaaag
     1081 cgattttaaa ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc aaccgagtat
     1141 ccccatgaaa gtgatctgcg ccgggcaaag cgacgctggc atggcgttct ccgcccagta
     1201 tgccgatttc aacttctgtt tcggcaaagg cgtaaataca cccacggctt tcgccccgac
     1261 cgctgcgcgg atgaaacagg ccacagagca aaccggacgc gacgttggct cttatgtgtt
     1321 gtttatggtg attgccgatg aaaccgacga tgccactcgc gccaaatggg aacactacaa
     1381 agcgggcgcg gatgaagagg cgttaagctg gctaaccgaa caaagtcaga aagatacccg
     1441 ctccggtact gacaccaacg ttcgtcagat ggccgatccc acttcggcgg taaacatcaa
     1501 tatggggacg ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag
     1561 cgtgcctggt gcagaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac
     1621 cttcggcgag cgcattcaac cactgatgca gtgccgcgcc catctctctg cgctgactca
     1681 ggaggtggca tgatgacaac cttaaccgct cgaccggaag ccattacctt cgatccgcag
     1741 caaagtgcgc agatcgtggt ggatatgcaa aacgcttatg ccacgccagg cggctactta
     1801 gatctcgccg ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgct
     1861 gtgaccgcag cgcgagcggc agggatgttg atcatctggt ttcaaaatgg ctgggatgaa
     1921 cagtatgtcg aggctggcgg ccccggctca ccgaattttc ataaatcgaa cgccctgaaa
     1981 accatgcgta agcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat
     2041 caactggtgg atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac
     2101 agcggtttct tcaatacgcc gctggacagc attttacgca gccgtggaat acgccatctg
     2161 gttttcaccg gtatcgctac caacgtctgc gtcgaatcga cgctacgcga tggctttttt
     2221 ctggagcatt tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca
     2281 cagaaagccg cgttgttcaa tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg
     2341 ttctgcgacg cgctttctcc cacgtccttt gctcgtatcg cttaaggagt ttagcgatgc
     2401 caaaatccgt aattattccc gctggcagca gcgcaccgct ggcccccttc gttcccggca
     2461 cgctggctga tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataataacg
     2521 tgctgtttgc cgatgaccca aaggcgcaaa cccgccacgt tctggaaact atccgcaagg
     2581 tgatcgagac ggcgggtggc acgatggcgg atgtgacctt caacagcatc tttattaccg
     2641 actggaaaaa ttacgccgcg attaacgaaa tctacgccga gttttttccg ggtgataaac
     2701 cggcgcgatt ctacattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca
     2761 caattgcgca tatcgccaag tgaggccgcg atgaaacttt cactctcatc tcccccttat
     2821 gctgatgcac ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta
     2881 ccgcaactgg cggtgctgga gcaggagtat caggtagtct gttacgacca gcgcggcacc
     2941 ggcaataatc ccgacacact ggcagaagat tacagtatcg cccagatggc agcggaactg
     3001 catcaggcgc tggtagccgc agggattgag cgttacgcgg tggtcggcca tgcgctcggt
     3061 gcgctggtgg ggatgcagct ggcgctggat tatcccgcgt cggtaactat gctggtcagc
     3121 gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacga
     3181 ttactgtata gcggcggcgc gcaagcatgg gtggaagcgc agccgttgtt cctctatccc
     3241 gccgactgga tggcggcccg cgcacctcgc ctggaggcag aagacgcgct ggcactggcg
     3301 cattttcagg gcaaaaataa tttactacgt cgacttaacg ccctcaaacg cgctgacttt
     3361 agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg
     3421 ctggtgccaa cagcatgttc cagtgaactt catgccgccc tgcccgatag ccagaaaatg
     3481 gtgatgccct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg
     3541 ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta aggaattgct
     3601 atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac
     3661 aacggctggc gggagacacc cgtcagcgat gagacgttac aggagcttta tgccctgatg
     3721 aaatgggggc cgacatcagc taactgttct ccggcacgga tcgtgtttat ccgcactgca
     3781 gaaggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa aaccctgacc
     3841 gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt accactactg
     3901 tttccccacg gtgatgcccg cagttggttt acctccagcc cacaacttgc cgaagaaaca
     3961 gcgtttcgca acagttccat gcaggcggct tatctgatcg tcgcttgccg tgcgctggga
     4021 ctggataccg gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc cttttttgcg
     4081 ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag cagcaaactt
     4141 tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac
     4201 tatgaacata gttgatcaac aaacctttcg cgatgcgatg tcctgtatgg gcgcggcggt
     4261 caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg
     4321 cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg
     4381 gccggtattc aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg ggcaggagcc
     4441 gctttcaaat ctttttggcg gcaaaacgcc catggaacac cgctttgccg ccgcccgctg
     4501 gcagacgggt gtgaccggat gcccgcaact ggaagaggct ctggtttcgt ttgactgccg
     4561 tatcagccag gtggtgagcg tcggcaccca cgacattctg ttttgcgcca tcgaagcgat
     4621 tcatcgtcac accacaccct acgggctggt gtggttcgat cgcagttatc acgcgctgat
     4681 gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg ttttcctcac
     4741 tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga acgattgccg
     4801 tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg
     4861 ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc ggggatcggc
     4921 acgttactgt ttttctttat taccggcggg cgcgtcccca gttatctcgg ctccagcgcg
     4981 gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat taacccgaat
     5041 atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt cattggcctg
     5101 gtggtgatga aaattggtac acgctggatt gaacgactaa tgccgccggt ggtgacgggt
     5161 gcggtggtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaag tgtatcggct
     5221 tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct ggtggcggtg
     5281 tttacccgtg gcatgatcca gcggttattg attctcgtag gattaattgt cgcctgtctg
     5341 ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc
     5401 agctatgctg cctggttcgg tctgccgcat ttttccactc ctggttttaa tggccaagcg
     5461 atgatgttga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag
     5521 gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc attcgtcggt
     5581 gatggtctgg caacgatgct ttccggctct gtcggcggta gcggcgtcac cacctatgct
     5641 gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt tgttgcagct
     5701 gccgtcatcg ctatgctgtt gggcttttcg ccgaaatttg gcgcgctgat ccataccatt
     5761 ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc
     5821 gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acgctaattt gattatggtc
     5881 gcagtaacct tcgtgctggg cgcgggtgat tttgctctca cgcttggcgg ttttacgttg
     5941 ggagggatcg gtacagcaac ctttggcgcg attttactca atgcgttatt aagcagaagg
     6001 ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga aaaccgccgg
     6061 gttaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgcattcg tcattgatta
     6121 gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac ctgcttctga
     6181 tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga
     6241 cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca
     6301 tattttcgct acgtgggact aagcttagtg aactgacgcg gacagacccg cgtttggtaa
     6361 tatcctgcaa cagcttctta gcgagaatag atatcatctc caataattat ccataagccg
     6421 ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt
     6481 gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac
     6541 gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg
     6601 ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg
     6661 gcacgcgcag tcgctgaggg tgcaagcaaa gtggatggcg cagaagttgt cgttaagcgt
     6721 gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca
     6781 ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc
     6841 cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg
     6901 gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc
     6961 ggtcaggaac aaaccatcac atccacctgg acgacccttg cgcatcacgg catggtgatt
     7021 gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg
     7081 ccgtacggcg caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa
     7141 ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc
     7201 taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg
     7261 aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg
     7321 ggtacgacga aaaaatggca gaaaagattt gggaagaagg tagtgatgaa gtgttagtca
     7381 aagcctttgc taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta
     7441 aaaacgttta accagcgact cccccgcttc tcgcggggga gttttctgtt atttcaccgc
     7501 ctcattcaac acgctatcaa acttatccat cgggcagaaa ccgttggcat ctatcgggca
     7561 accgcttaat tccagcgtca cgcgctgcgc aggtgcctgc agggttaacg catcggcatt
     7621 acgtaactgt tccgcactct ggtacacata ttcaattttc atcaaatcgc gattggcttt
     7681 gctgtcatgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc
     7741 atgcaactga tacggtttga aatccagcgc cgttaacaga gaggctatgt tggagtcgtg
     7801 cccaactaac accgtgattt tcggagcact ggcgcgatcg gtgaccaggg ctttgtcgat
     7861 ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acagactgtc
     7921 ctggtaaccg tttttcagct tcgacaatac cttccactgc tggtcagatt tgatttctcc
     7981 ccaggccacc tgatccatcg gaaaaccttc gtagtattgc aaagtaaacg catctaccag
     8041 cgagttgccg actttcagcg gcccggaaac acctggttct tgttgatact tggcgctaaa
     8101 ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt
     8161 atagttaacg attttttcca gtagctggta gctgtcggta agctggagtt tgctgagctc
     8221 tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcatcgg
     8281 gttaaaggtt gggtccatag tgcccatttt ttcctggtga tgcacaggaa tatcacaccc
     8341 agggaatgca ccagtaataa agaactgggc ggtagcgacg gtacgttgca gactattggc
     8401 ataggcataa acggtgtccg gcggcgggca ttcccccgat ttcactatcc cctgctctgc
     8461 cagccattca cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag
     8521 ttgcccaccg gggacgtccc attcgggcca tttattcggc gtcgactgct ccagcacact
     8581 gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca cttgctgtag
     8641 ctgatagcct tccggtacgg tttgtgcctg agcatttgaa gcgagtaaaa ctatccctgc
     8701 cacagttgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcatta
     8761 aagagtgtga cagaaatatg acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa
     8821 cgcctgtttt tctatataca tcttcaaaaa caggcaaggt ttgacgcaaa ctatgcctga
     8881 ttagcccctg cctcgcgtac gccgctgata attgcacctg tggctaaaca gaatatctgc
     8941 ggcgttacca atacagagac aatatttacc aataaaaaag tgtgagacta ataacaatgg
     9001 tttttagcat atgcctttgc cattaagttg cataacgatt taaatagtca agttgcgcat
     9061 tcatttcctt attttttaac ttataagcct gagaaaaaag ttcaggaggg aggtaaacat
     9121 cataatgatt gccagtgttt gttaaatatc catactcaag atcttcatct gttgaaaagg
     9181 attcatttcc caaggagatt gatgccacat tactattaac aataaataat gagcaaatgc
     9241 tatctgaaag aacagcaaga aaattcttca ccgctttaat cctatccgac gtgtcggtat
     9301 ctaaagcaag caaattcttg ttatcattaa atataaactc gggtaaatgc agtatgcatt
     9361 ttttgtcatt caagcaacta tcaatgaatt gttttttaaa aaacagaaag ctccatgaca
     9421 taaaactacc cacaccctga attttgttgt cagattgcag atcaacgcca aataatgtct
     9481 ttacatcttc cctgagatta tagaatgccg tatcaacaag agcctcgatc gtttgtttat
     9541 caacaatttt aataaactct tcgtatactt gcttttgcgt cttatggctg cccagcgccc
     9601 atgaatactc atgttctttt aaaaccagta tggctcttaa cagacaatat ccatcgccat
     9661 ctactggagc gtattttaat ccatatgttt tattaatttc actctgaata aaagatatgc
     9721 cagacaaatc aataccagta tctaaatcag atattcccgg catatttatc tgttctggct
     9781 ggatactcat attttcatta tctaaacaat actcagaaaa aacatcatca cacgcattaa
     9841 tgttttcaat atattccata aagccatacc aataataacc tttaaaatct gtgatagact
     9901 ctgaacctct gcccggcaag gacactctaa caatatcaac atcttgaaca tattgtatag
     9961 tatatgtaat acctcctgaa acccctgaat aaatttgtgt atcattagta cgcaatgttc
    10021 tcataaataa tgatagatca ttatcattct gcatttcctc aaattctttt tctagtgatt
    10081 cacattccag acatggagta tcctcttcat ttactttacg tctgagttca ttgtacaacc
    10141 gcataaaaca caccagtgtg ttttcaaaaa gcgtgattga atatgtttga tttttttcgt
    10201 actcattttt tatatcgttc caggcatgtt tcaaataatt atttgcagtg caactaattc
    10261 catgtatatt actacccata tatagcgtct ataaaattta ataaataatg acgccctagt
    10321 taaacttaaa gtgcctggtt caactatcaa aaatcgctca ccctttttca cctgtttaaa
    10381 atatgttcag caacccatct tgatggcgac ctcctctccg cgatgatttc aataacatat
    10441 tctgtgttgg catatgaaat tttgaggatt accctacact tataggagtt accttacagg
    10501 ggttccttca atttgtgttg atttacgcga gataacgcta tggaattaaa ggattattac
    10561 gccatcatgg gcgtgaaacc gacggacgat ctcaagacaa tcaagaccgc ctatcgtcga
    10621 ctggcccgta aataccatcc tgatgtcagc aaagaaccgg atgccgaagc ccgcttcaaa
    10681 gaggtcgctg aagcctggga agtgttaagt gatgaacaac gtcgcgctga gtatgatcag
    10741 atgtggcaac atcgcaacga tccgcaattt aaccgtcagt tccatcatgg cgacggtcag
    10801 agttttaatg ccgaagattt tgacgatatc ttctcgtcaa ttttcggtca gcatgcccgc
    10861 cagagccgtc aacgccccgc cgcacgtggc cacgatattg aaatcgaagt ggcggtattc
    10921 ctcgaagaaa cgcttactga gcataagcgt accatcagct ataacctgcc ggtttataac
    10981 gcctttggca tgatcgaaca ggaaatcccg aaaacgctga atgtgaagat cccggcaggc
    11041 gtcggcaatg gtcaacgcat ccgcctgaaa ggccagggga cgccgggcga aaacggcggt
    11101 ccaaatggcg atttgtggct ggtgattcat attgcgccac atccgctgtt cgatattgtc
    11161 ggccaggatc tggaaattgt ggtgccggtt agcccgtggg aagcggcgct gggtactaaa
    11221 gtcaccgttc caacactgaa agaaagcatt ttgctgacta tcccgccagg cagccaggcc
    11281 gggcaacgat tgcgcgttaa aggcaaaggt ctggtgagca aaaaacagac cggcgatctg
    11341 tatgcggtac tgaaaatcgt gatgccgccg aaaccggatg aaaacactgc cgcgctgtgg
    11401 cagcaactgg cagacgccca gtcgtctttt gatccacgta aagattgggg gaaagcataa
    11461 tggctaatgt tacggtgact tttactatta ccgaattttg cctgcatacc ggcatctctg
    11521 aagaggagtt gaatgaaatt gtcggtttgg gggtggttga accgcgtgag atccaggaaa
    11581 caacctgggt atttgacgac catgccgcca ttgtggtgca acgcgcggta cgcctgcgtc
    11641 atgaactggc tctggactgg ccggggatcg cggtggcgct gacgttaatg gatgatattg
    11701 cgcacctgaa gcaggaaaac cgcctgctac gccagcggct ttcccggttt gtagctcatc
    11761 cgtgatgtgt gtttgccgga tgtgatgacg catccggcaa ctgcgccata atgcctgatg
    11821 cgacgctaaa cgcgtctttt caggcgcata aacgacgtta tctgttttgg tggtcgcact
    11881 ccactaacac cagcaataac tggcttagtg ccgcgtaaaa accaaagctg tcatactgtc
    11941 gacaacgcgc agcaaactct ggtaaccatt gccgcagcgc cgtcagtgtt ttttgccgca
    12001 aactgtcgat tcttcgcgca ggaacggtcc cctctcccag cgaaaaatgc agataactca
    12061 acaactcgag atagatcgcc agatgatctg ccggt
//
LOCUS       DAJVPT010000109        12062 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVPT010000109 DAJVPT010000000
VERSION     DAJVPT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OQM47_003182"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQM47_003182"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OQM47_003183"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OQM47_003183"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1481231.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OQM47_003184"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OQM47_003184"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1481232.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OQM47_003185"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OQM47_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OQM47_003186"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OQM47_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1481233.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OQM47_003187"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OQM47_003187"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1481234.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OQM47_003188"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OQM47_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1481235.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OQM47_003189"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OQM47_003189"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCS1481236.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OQM47_003190"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OQM47_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1481237.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OQM47_003191"
     CDS             9553..10332
                     /locus_tag="OQM47_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1481238.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OQM47_003192"
     CDS             10322..11131
                     /locus_tag="OQM47_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1481239.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OQM47_003193"
     CDS             11218..11652
                     /locus_tag="OQM47_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1481240.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OQM47_003194"
     CDS             11649..>12062
                     /locus_tag="OQM47_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1481241.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3251 c         3078 g         2855 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggt gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJVPT010000110        11960 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVPT010000110 DAJVPT010000000
VERSION     DAJVPT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1170)
                     /gene="uxaB"
                     /locus_tag="OQM47_003195"
     CDS             complement(<1..1170)
                     /gene="uxaB"
                     /locus_tag="OQM47_003195"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1481242.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLT"
     gene            complement(1397..2815)
                     /locus_tag="OQM47_003196"
     CDS             complement(1397..2815)
                     /locus_tag="OQM47_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1481243.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(2969..3313)
                     /locus_tag="OQM47_003197"
     CDS             complement(2969..3313)
                     /locus_tag="OQM47_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1481244.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3313..4239)
                     /gene="glsB"
                     /locus_tag="OQM47_003198"
     CDS             complement(3313..4239)
                     /gene="glsB"
                     /locus_tag="OQM47_003198"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1481245.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4303..5238)
                     /locus_tag="OQM47_003199"
     CDS             complement(4303..5238)
                     /locus_tag="OQM47_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1481246.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5318..6433
                     /gene="yneK"
                     /locus_tag="OQM47_003200"
     CDS             5318..6433
                     /gene="yneK"
                     /locus_tag="OQM47_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1481247.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6583..7773
                     /gene="ydeA"
                     /locus_tag="OQM47_003201"
     CDS             6583..7773
                     /gene="ydeA"
                     /locus_tag="OQM47_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1481248.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7798..8463)
                     /gene="marC"
                     /locus_tag="OQM47_003202"
     CDS             complement(7798..8463)
                     /gene="marC"
                     /locus_tag="OQM47_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1481249.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8675..9109
                     /gene="marR"
                     /locus_tag="OQM47_003203"
     CDS             8675..9109
                     /gene="marR"
                     /locus_tag="OQM47_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1481250.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9129..9512
                     /gene="marA"
                     /locus_tag="OQM47_003204"
     CDS             9129..9512
                     /gene="marA"
                     /locus_tag="OQM47_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1481251.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9544..9762
                     /gene="marB"
                     /locus_tag="OQM47_003205"
     CDS             9544..9762
                     /gene="marB"
                     /locus_tag="OQM47_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1481252.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9793..10692)
                     /gene="eamA"
                     /locus_tag="OQM47_003206"
     CDS             complement(9793..10692)
                     /gene="eamA"
                     /locus_tag="OQM47_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1481253.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            10956..>11960
                     /gene="ydeE"
                     /locus_tag="OQM47_003207"
     CDS             10956..>11960
                     /gene="ydeE"
                     /locus_tag="OQM47_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1481254.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     T"
BASE COUNT         3151 a         3082 c         2731 g         2996 t
ORIGIN      
        1 agtaaggcgc gccggaagtg tgccatttgc cttctgccct gccagcagct gcggcaggat
       61 gcgggtgcgg aacttggtca taccgttgag cgcgatagac agcagctgat gcttaatgta
      121 cgggttacgg aaacgcccgg tgactgcact ggcgaaagat tccagttcat cacgaggcaa
      181 atccagtacc ggaataattt cttcgtagat ggctttttca acgaatgcgc aaatttcagc
      241 atcgttcatc gcttcaccta cggtatccag ccctgcctgg aacgccaccg gcaccagcgc
      301 ggtgtgcgca ccgttgagga ttgccacttt gcgttcttta tacggtttaa tatcgtcaac
      361 aatcagcacg ttgagcggat atttgtccag gcgcagctca gtcgctaagg atttcggccc
      421 ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt gataacccag
      481 ttcttcttcc agtttagcca cttcatcgcg cggataaccg gtaacgatac ggtccaccag
      541 cgtagaacag aagctgttag cctgatccag ccattgaata aatgcttctg gcagtgccca
      601 ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg ccattgtagt caatcaactc
      661 acacggaatg atgatccaac ctttatccag cgcaccgttg aaatggctaa agcgttcgaa
      721 cagcagacgg gtcagttttg ccggatagct taccgctggc gcgtcatcga atttatcgcc
      781 cgcatggtag ctgatacccg cttcggtggt gttagagaaa acaaagcgca tttccgggtt
      841 gtgcgccagt ttcaggaatt catcgtattc actgtagacg ctgatttcac gattaaccga
      901 gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca ttcaggccac ggataatggt
      961 ggtgtacaga ccatcctgcg tgctcagtga cggcgggaat gaagtttcaa tcggacgaac
     1021 aacgaccacg ccagaattca gatcggtgtg ctcattcagg agatcgattt gccagtcaac
     1081 aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata cgttctggat actgtgcacc
     1141 gggaaaatcg cgacgattta gtgttttcac aatgggttcc cttctgatta gtcatacaac
     1201 ccgtttgaat tggtgcgaca ggttagcaaa ctttaatacg ccgaacccct gttttgatca
     1261 actcctgatg attaatgagc agttttatga gaaaagtgtg gcgcggatca tggtttaatc
     1321 gaggaaatac gccttttcct ggatcataaa gtggtagaac acattgcatt caaatcgcgc
     1381 ggaatgaata aagatgtcag acaacttcct caccgtaacg catagtgctg gtacggttgc
     1441 gcccatcttt cttcgagcga tacagacacg tatcggcttc gaccatcaat ttattaaaat
     1501 catcggtcag cgttcggtac gatgcgcgac cactaccgac gccaatactt accgtgagat
     1561 aaagcgtttt ttgttgccag gtgaatggtt gcagttcaac gcctttacgg attttttccg
     1621 ccattagcag accatcgaca ggattcaccg acggcactgc aacagcaaat tcttcgccgc
     1681 ccatgcgcgc caccagcccc ttatcaccga caatcttctg aatatgccgg gcaaacacgc
     1741 ttaacacttt atcgccacat tcatgtccat agttatcgtt aatgcttttg aaatagtcga
     1801 tatcaagcag catgaccgtc agatgttgtg tctgtttcag cgatggactt ttcagcacct
     1861 cataaagacc ggaccgtgag taaacctgag tcagaaaatc aaagtcggct cgcagcgcaa
     1921 cttgcttcat tagcaaattg atcgctgcca cgctaaaaga aaccataatt gggcatatcg
     1981 ccatcgtggc aataccgaga cgtgcggaga acatttccgg aatggagaac ggcgaaccga
     2041 ccgagatatc aatcaccgaa tttgccacca gtacgatttc caccgcaccg gtgacaaagg
     2101 tcaacagaca tgttacctgc ggcgtatagc gcactgcaca ccagattaat gcaggcagcg
     2161 ggaacgccag actccccgct ccgccaatga ctaccgaggc aataaccgac acaataaacg
     2221 cgatggcggg catcatctgt tctgctttaa agcgcggcag cactccagga atagccaacg
     2281 tcagcataca aggcacgatc aacacgcccg ttgagaattg ctcactgaac cagtccgcaa
     2341 gcaaaggcca gaaatccaga ctatcaatac tgaccgaacc aatcgccccg acaatagcgc
     2401 ataataatgc ggcaatcaga cagtaattaa atagccgtaa agcacttact ggctcatact
     2461 tatttttccc cagacgctta tcccgcacga ccagtaaggc gacggtaaca ataaacatca
     2521 tattggagaa attgataacc agtgaaacca gtccccattc ggtggttatg gcatcataaa
     2581 ccagcatcgc cacataactg atcgcatagt aatgcagtcg attaagccag acatagcggg
     2641 caaacacccc cgccattacg ccattcagcg gccagaaaag agaaagttcg tgaaccagcc
     2701 gtaactctgc accaataaaa tagaacagcg tggttagcgc aaaaatagcg atgctattac
     2761 gtagcagatg accttcctgg aacaaacgga aagttgaaat ggggtgtaca tgcattaatt
     2821 atctgtccat acaattgcgg gtggcaatta tctcaacgtg ataagtaaat tctcataaag
     2881 gatagcatgt tcgctaacag cggcggcaaa taaaggtacc ctgggaatgc tattttatcg
     2941 ttttccgcaa ttctcatggc tgattcatct atacaaccag ccagtgatag attaccgcca
     3001 cgatataacg ttgttgttct tcgcttaacg aaacaccctg cggaatgtta tgccacttag
     3061 ccagacaacc gcgacagcag gtcgcggtgg cgtgctgagc gataaacacc ggatgcccgc
     3121 gcatgggggt ttgcttaccg tcattagccg gtaatgccgg agcaagacgt ttagcgacaa
     3181 aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac tgacgctctt
     3241 tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg aagagtggat
     3301 cgagagactg cattaataaa ccgaacgccc taactgtttc gtcaattgtt caaggacggc
     3361 gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg cgatggccat
     3421 ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg gtagtcccac
     3481 ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca gcgcgttaat
     3541 ttgccgcgcc tgcattggcg tcaccacagg ttcatcaata tgaatagctt tcccctgatt
     3601 agccagaaag acaaacgtcc gggccagctc tacacagctc attttcagag cgcagtaatg
     3661 aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg acttcatcag
     3721 ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta ccaccgtatc
     3781 gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac gttgccgtgg
     3841 tgcgcttagt cgcccttgca acatatcgca gaccaccagc gcaccggtat taatgaacgg
     3901 attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg gtgatccaga
     3961 cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca tggcgacaac
     4021 gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt ctccggcctg
     4081 aaaaagctgt ccgtcaacgg tacagatagc aatccccaat cgggaaccgt ctactgtagc
     4141 cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca cttgccgcaa
     4201 gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct ggaccgcagg
     4261 tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc ggtctttcca
     4321 cactgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac gaccaaagcc
     4381 actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac cattgataaa
     4441 caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt cggtggtaaa
     4501 aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat gttctgcatc
     4561 tttcgcaatg gtgattgcag caacggggcc aaacatttct tcccgaaacg cggtcatttc
     4621 tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgctccag ccatcttttc
     4681 cccgcccagt aacaaacgcg caccctgcgc cagggttttc tccacctgat gatgcagctc
     4741 atcacgtaaa tcaaaacgag ccattggtcc gagagcgttc tcttcatcac ggggatcgcc
     4801 cattttcaag gctgccgcag ctgacacaaa acgttcggta aatgccgaag caattccctc
     4861 ttcgataata aagcgttttg ccgctgcaca aacctgtccg gtattctgat aacgtccggc
     4921 taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa atggatccga
     4981 accgcccagt tccagtacgc attttttcag tgccgctcca gcacgaacac ttccggtcac
     5041 cgtgacagca gcaatgcgcg agtctttaat catctgactg acaccgtcgt tgtcggcatt
     5101 cagccagcca tatacgcctt gcgggattcc cgcatcttta aacacttggg caatgagctg
     5161 agcacagccc atcacattcg gcgcatgttt aagtaagtag ccgttacctg caagaatgat
     5221 gggaacagcg ccacgcatca cctgcctttg atagttcttg cagctcaaat gccctatata
     5281 gtgatgttcc gatgacttat gactatatgg ggcaaatatg gttacgccag taagcatctg
     5341 caattacata tctcttcctg atgattttcc cgctcgtaat attgcacctc aagtaaaaga
     5401 agttttaaag gattttattg atgcactcag taccataata tgtgatgaag agtggcgcac
     5461 gtctttaaac accaactcag ccacaaaaaa gatatttaac aaccttgata acttatcata
     5521 tattcagagg acttcttttc ggggtaacga cacactatac aatgaaaagg ttcagtttaa
     5581 actcacttat cctgtcaaga atggaagaca caaagaaaat attgaatttc aggtagtaat
     5641 aaatttaagc cctatttatt tagataattt tcgccatgat ggagaaatta atattttttg
     5701 cgctcccaac ccaaagcctg tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt
     5761 gctctttctg tttatgaatg attttattga acaatttcca atgatcaacc ttggcgcccc
     5821 catcaaaaga gcgcatacac cacatattga acccctgcct ccggatcacc ataccgctgc
     5881 agattactta cgccagtttg atttgcttgt cctgaatttt atctctcgcg gtaattttgt
     5941 catactcccc cgattatgga ataactctga ggttcacaga tggttcgtca ataaagatcc
     6001 taacttgatc accgccattc tcgacataac ggacagcgaa ttaaaagagg atttattgca
     6061 aagcctgatg gattcattag gttctaacaa acatgtacaa cccgaagtct gcatctgctt
     6121 cttatccctt ttagcagaac aagaatctcc tcattttcag gacttgtttt tatttttcgc
     6181 caatatgtta ttgcactatc accaatttat gaatcccaat gaaagtgatc tgaatgacgt
     6241 gttgatgcca gcatcattaa gtgatgataa aattatcaaa catatggcgc gcaggaccct
     6301 caaactgttt gtaaaaaatg aaacgccgcc aaaagttact cacgaagacc tggtgaaaaa
     6361 caggcctcgc tcccctgtca gaccacctat acccgcaacc gccaaaacgc cagacctccc
     6421 tgaacgtcat taaaccgtga tgttaccgac tctctgactc ggaacagaat cagcgtcaga
     6481 gaaacggaaa acgcgatcca gatcacaaat gcattgtatt cacatcatta accgttttaa
     6541 gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac aaacactgtt
     6601 tcccgcaaag tggcgtggct acgggtcgtt acgctggcag tcgccgcctt catcttcaac
     6661 accaccgaat ttgtccctgt tggcctgctc tctgacattg cgcaaagttt tcacatgcaa
     6721 accgctcagg tcggcatcat gttgaccatt tacgcatggg tagtagcgct aatgtcattg
     6781 ccttttatgt taatgaccag tcaggttgaa cggcgcaaat tactgatctg cctgtttgtg
     6841 gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gctttaccgt tctggtgatc
     6901 agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc gtctctggcg
     6961 atccgtatgg ctccggccgg gaagcgggca caggccttga gtttaattgc caccggtaca
     7021 gcactggcga tggtcttagg tttaccgctc gggcgcattg tggggcagta tttcggctgg
     7081 cgaatgacct tcttcgcgat tggtattggg gcgcttgtta cccttttgtg cctgattaag
     7141 ttacttccct tactgcctag tgagcattcc ggttcattga aaagcctccc gctattgttc
     7201 cgccgcccgg cattgatgag catttatttg ttaactgtgg tggttgtcac cgcccattac
     7261 acggcataca gctatatcga gccttttgtg caaaacattg cgggattcag cgccaacttt
     7321 gccacggcat tactgttatt actcggtggt gcgggcatta ttggcagcgt gattttcggt
     7381 aaactgggta atcaatatgc gtctgcgttg gtaagtacgg cgattgcgct gttgctggtg
     7441 tgtctggcac tgctgctacc tgcggcgaac agtgaaatac acctcggggt gctgagtatt
     7501 ttctggggga tcgcgatgat gatcatcggg cttggtatgc aggttaaagt gctggcgctg
     7561 gcaccagatg ctaccgacgt cgcgatggcg ctattctccg gcatatttaa tattggaatc
     7621 ggggcgggtg cgttggtagg taatcaggtg agtttgcact ggtcaatgtc gatgattggt
     7681 tatgtgggcg cggtgcctgc ttttgctgcg ttaatatggt caatcattat atttcgccgc
     7741 tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccattcgg gcctttttta
     7801 atggtacgtt ttaatgattt ccaggatgcc gttaataata aactgcacac ccatacatac
     7861 cagcaggaac cccatcaggc gggagatcgc ttcaatgcca cccttgccca ccagccgcat
     7921 aattgcgccg gagctgcgta ggcttcccca caaaataacc gccaccagga aaaagatcag
     7981 cggcggcgca accatcagta cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc
     8041 cgagctaata atcatcgcta tggttcccgg accggcggta cttggcattg ccagtggtac
     8101 aaaggcaata ttggcactgg gttcatcttc cagctcttcc gacttgcttt tcgcctccgg
     8161 tgaatcaatc gctttctgtt gcggaaaaag catccgaaaa ccgataaacg cgacgattaa
     8221 gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca ccagttgccc
     8281 agcgtaatac gccaccatca tgatggcaaa tacgtacacc gaggccatca acgactgacg
     8341 attacgttcg gcactgttca tgttgcctgc caggccaaga aatagcgcga cagttgttaa
     8401 tgggttagct aacggcagca acaccaccag ccccaggcca attgctttaa acaaatctaa
     8461 cattggtggt tgttatcctg tgtatctggg ttatcagcga aaagtataag gggtaaacaa
     8521 ggataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt gaaccgattt
     8581 agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctgg gcaatattac
     8641 cccctacaac taattacttg ccaaggcaac taatgtgaaa agtaccagcg atctgttcaa
     8701 tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag atcgtctgct
     8761 taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc tctgctctat
     8821 ccgctgcgcg gcgtgtatta ctccggttga actgaaaaaa gtgttgtcgg tcgacctggg
     8881 agcactgacc cgtatgctgg atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa
     8941 cccgaatgat aagcgcggcg tactggtaaa acttaccacc agcggcgcgg caatatgcga
     9001 acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa acctgacggc
     9061 ggacgaagtt gcaacacttg agcatttgct taagaaagtc ctgccgtaaa caaaaaagag
     9121 gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt ttggactgga
     9181 tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt tcgggttact
     9241 ccaaatggca cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta ggccaataca
     9301 tccgcagccg taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac
     9361 tctatctggc agaacgatat ggcttcgagt cgcaacaaac tctgacccga accttcaaaa
     9421 attactttga tgttccgccg cataaatacc ggatgaccaa tatgcagggc gaatcgcgct
     9481 ttttacatcc attaaatcat tacaacagct agttgataac gtgacaacgt cactgaggca
     9541 atcatgaaac cactttcatc cgcaatagca gctgcgctta ttctcttttc cgcgcagagc
     9601 gttgcggaac aaaccacgca gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc
     9661 ccatcgcagg aacaaccacc gtttgattta aatcacatgg gtactggcag tgataagtcg
     9721 gatgcgctcg gcgtgcccta ttataatcaa cacgctatgt agtttgttct ggccccgaca
     9781 tctcggggct tattaacttc ccacctttac cgctttacgc caccgcaagc caaatacatt
     9841 gatatacagc ccggtcataa tgagcaccgc acctaaaaat tgcagacccg ttaagcgttc
     9901 atccaacaat agtgccgcac ttgccagtcc gactacgggc accagtaacg ataacggtgc
     9961 aacccgccag gtttcatagc gtcccagtaa cgtcccccag atcccataac caacaattgt
    10021 cgccacaaac gccagataca tcagcgacaa gatggtggtc atatcaatag taaccagact
    10081 gtgaatcatg gatgcggaac catcgagaat cagcgagaca acaaagaagg gaatgattgg
    10141 gattaaagcg ctccagatta ccagcgacat caccgccgga cgcgttgagt gcgacatgat
    10201 ctttttattg aagatgttgc cacacgccca actaaatgct gcagccaggg tcaacataaa
    10261 gccgagcatc gccacatgct gaccgttcag actatcttcg attaacacca gtacgccaaa
    10321 aatcgctaag gcgatccccg ccaattgttt gccatgcagt cgctccccga aagtaaacgc
    10381 gccaagcacg atagtaaaaa acgcctgtgc ctgtaacacc agcgaagcca gtccagcagg
    10441 cataccaaag ttaatggcgc agaacagaaa ggcaaactgc gcaaaactga tggttaatcc
    10501 ataccccaag agcaaattca gtggtacttt cggtcgtgca acaaaaaaga tggccggaaa
    10561 agcgaccagc ataaagcgca aaccggccag catcagcggt ggcatgttat gaagccccac
    10621 tttgatgacc acaaaattta gcccccatac gaccactacc agtagcgcca acaccccatc
    10681 ttttcgcgac attctaccgc ctctgaattt catcttttgt aagcaatcaa cttagctgaa
    10741 tttacttttc tttaacagtt gattcgttag tcgccggtta cgacggcatt aatgcgcaaa
    10801 taagtcgcta tacttcggat ttttgccatg cgtccgatgg ttaataacaa cgacgaggca
    10861 agaagggcgc tggtagagcg tcgtagggat aagttcattt gtctgtccgg cagtgcgttt
    10921 cgttatgttt tattttagag atgtaaagaa atagcatggc tacgctgcca tttatgacca
    10981 tttacttgag tcgccagtac agcctgagtg tcgatctaat cggttatgcg atgacaattg
    11041 cgctcactat tggcgtcgtt tttagcctcg gttttggtat cctggcggat aagttcgaca
    11101 agaaacgcta tatgttactg gcaattaccg ccttcgccag cggttttatt gccattcctt
    11161 tagtgaataa cgtgacgctg gttgtgctct tttttgccct cattaactgc gcctattctg
    11221 tttttgctac cgtgctgaaa gcctggtttg ccgacaatct ttcgtccacc agcaaaacga
    11281 aaatcttctc aatcaactac accatgctaa acattggctg gaccatcggt ccgccgctcg
    11341 gcacgctgtt ggtgatgcag agcatcaatc tgcccttttg gctggcagct atctgttccg
    11401 cgtttcccat gcttttcatt caaatatggg taaagcgcag cgagaaaatc atcgccacgg
    11461 aaacaggcag tgtctggtcg ccgaaagttt tattacaaga taaagcactg ttgtggttta
    11521 cctgctctgg ttttctggct tcttttgtaa gcggcgcatt tgcttcatgc atttcacaat
    11581 atgtgatggt gattgctgat ggggattttg ccgaaaaggt ggtcgcggtt gttcttccgg
    11641 tgaatgctgc catggtggtt acgttgcaat attccgtggg ccgccgactt aacccagcta
    11701 acatccgcgc gctgatgaca gcaggcaccc tctgtttcgt catcggactg gtcggtttta
    11761 ttttttccgg caacaacctg ctattgtggg gtatgtcagc tgcggtattt actgtcggtg
    11821 aaatcattta tgcgccgggc gagtatatgt tgattgacca tattgcgccg ccaggaatga
    11881 aagccagcta tttttccgcc cagtctttag gctggcttgg tgccgcgatt aacccattag
    11941 tgagtggcgt agtgctaacc
//
LOCUS       DAJVPT010000111        11779 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVPT010000111 DAJVPT010000000
VERSION     DAJVPT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..547)
                     /gene="envY"
                     /locus_tag="OQM47_003208"
     CDS             complement(<1..547)
                     /gene="envY"
                     /locus_tag="OQM47_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1481255.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKK"
     gene            complement(730..1620)
                     /locus_tag="OQM47_003209"
     CDS             complement(730..1620)
                     /locus_tag="OQM47_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1481256.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(1621..4589)
                     /gene="nfrA"
                     /locus_tag="OQM47_003210"
                     /pseudo
     CDS             complement(1621..4589)
                     /gene="nfrA"
                     /locus_tag="OQM47_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(4576..6813)
                     /gene="nrfB"
                     /locus_tag="OQM47_003211"
     CDS             complement(4576..6813)
                     /gene="nrfB"
                     /locus_tag="OQM47_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCS1481257.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(6963..8405)
                     /gene="cusS"
                     /locus_tag="OQM47_003212"
     CDS             complement(6963..8405)
                     /gene="cusS"
                     /locus_tag="OQM47_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1481258.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8395..9078)
                     /gene="cusR"
                     /locus_tag="OQM47_003213"
     CDS             complement(8395..9078)
                     /gene="cusR"
                     /locus_tag="OQM47_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1481259.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9235..10617
                     /gene="cusC"
                     /locus_tag="OQM47_003214"
     CDS             9235..10617
                     /gene="cusC"
                     /locus_tag="OQM47_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1481260.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            10641..10973
                     /gene="cusF"
                     /locus_tag="OQM47_003215"
     CDS             10641..10973
                     /gene="cusF"
                     /locus_tag="OQM47_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1481261.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            10989..11708
                     /locus_tag="OQM47_003216"
                     /pseudo
     CDS             10989..11708
                     /locus_tag="OQM47_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2825 a         3024 c         3103 g         2827 t
ORIGIN      
        1 atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt cgcagattcc
       61 agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg ctgctgcgta
      121 agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac aatacggtaa
      181 aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct ggattgctgc ttgctgtggt
      241 tggtggctag ccggaacact tccggtgtac ggctatgaca agcgataacc ggggttgcta
      301 gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga taatcgttga
      361 tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt tcaatgacgt
      421 tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta gcatggttat
      481 tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca ctgctgctca
      541 attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat gcttcctgca
      601 tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt tttagcaatt
      661 aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc cgacttacct
      721 ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca gagagaaata
      781 gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt ctgcgtccgg
      841 tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat aaacaatgcc
      901 gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt cacgctgttc
      961 agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc cggttgcttt
     1021 aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga aaaaactact
     1081 gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt ttaaccatgt
     1141 tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt ccgcgctgat
     1201 gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt gctggagatc
     1261 ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt tctggtgcat
     1321 aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct gttgcgcagc
     1381 tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat cgccgtaacg
     1441 ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca tcagcccctg
     1501 ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa tacctttcat
     1561 cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga acttacgcat
     1621 ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac gttgattaat
     1681 cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc aggcgtcgta
     1741 gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct gcgcgccctg
     1801 agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa tagtctgtcc
     1861 gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct gaatatcctg
     1921 gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg aaccgttcgg
     1981 gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc gcagcatggt
     2041 atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga ggaaaaagat
     2101 ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt tcaccggcat
     2161 catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga gcaggtcgcc
     2221 ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt agctacgata
     2281 gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg cgccggaacg
     2341 caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct catgcaaacg
     2401 gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatgc ctgattatca
     2461 atgtcatcaa tcacgagccg ggcgtagtgc tgcgtcgcag gcatgtcatc cagacgctgg
     2521 ttcacgtagg ccagttgtcg gatcagtgcc ggatcgtccg gaagcccttt atgcgcctgt
     2581 tcgagcattt cccgcgactg tgcgatatca ccgctatccc acaaggcgta accaagcgct
     2641 gcctgggtgt tgctattatt tggttccagt tccagcgcgg cgcgcaaatc actcaccgcg
     2701 gccggaacat tatgacgttg gcgataaatt gtcgcccgcg caacgtaaga gttggcagaa
     2761 ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga gttctggctg agctgggata
     2821 taacgtcgcg catgcagcca ccagtattga gcattgtttc ccagctcacg ttgttctgcc
     2881 tgttgaagcc agcgatcgcg agctgcgcca tttcccgctg cctgagcggt gttggcggcg
     2941 gcgagcagat catcattgct catgtcgtga agactgattt tctgccaggc cgccagtgcg
     3001 gtggcgtagt cctcaacctg atacgcctga taggctaccg cacgatgttg ccaggcgctc
     3061 ggttgtcgtt gttcggcctg aagccatgca tacaacgcca caccgggtag cgtgtcccga
     3121 taacactttg ccagacggtt ccaagcggca gcatcgtagg aaggcgacat atcgcccagc
     3181 aagcgaacta ttgccgggca attatctgca atacccggca actgactttg ccactgacgt
     3241 tgctccgcca gcggtaaggg tttcgataaa atcgccacct tcgccggcgt tgctaggtaa
     3301 ggatgacttt ccagcagaga cgccagtcgc gccattaaag tctggctgac acgcgcatcg
     3361 ccctggaaag gatagcgttg cagcaataaa tcggcagctt cgcgtgactg ctcgttctgc
     3421 atcagttgcc aggtcagttg atccaggcgg ggaagatttg tcggttcttg ctgatacagc
     3481 aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg ccacgctgac agcataacgc
     3541 tcctcaagca tttcattggc ggggagggtg gcgagcagtt tttgcgctgc gtcgtactga
     3601 ccttctttta acagcaccgg tagcgtcgcg ccaacaacat actggcggtt atcggcaaac
     3661 tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc tgtatttaga taacagatag
     3721 agccaacttt tctcttgtgc gcccgtggta aacagcggct tattttcaat gagataatgc
     3781 tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg cgtaagtaat atatgactga
     3841 ggatcggtga agatcccctg tgattgcagt gccaggatcc gatcgtccag ctgcccggca
     3901 agaagcacgt caaaccactg acggcgttct gccgcgctta atgtgttctg ctggcgtgct
     3961 tcattgtata gcgtatctgc ctgggatcat tgtttcaggt agattgcccg ttgcagcaga
     4021 tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg tcgcatcgtt cagttgcgct
     4081 ctggcgacag gtaattgtgc cagccgcagg gcattctgcc cgacttcact gcgacaacgc
     4141 agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca gttcttcaac agtcgtaacg
     4201 cttttcactt caactggaat agccgccaga ctgcgctcaa gtcgggcatc tcctgggtga
     4261 cgtttcagtt gatcctcaag caacagccgc gcccggtcat catgaccaaa atgacgatag
     4321 gcttccgcaa ggtataaagt cagcggaata ttatccggca cctgttggtg tatatattca
     4381 aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg ccttcagcgc cttatcgaga
     4441 cggggataaa taacaaaatg gcgataatcg ctcagcccca gctcttctgc gctggtgccg
     4501 atattgtctg cgagtgcgct ggtactcaat aaagacgtca gcagtaaacc agaccatccg
     4561 atgacgcgat taaggttatt ctccttcatt ttcggactcc agttgcgcaa cctgttctgt
     4621 gtttaaacct gctttgagta atagtgattg catcgaaact tgtaattcgc gttgaattgt
     4681 caggacgcga tccaacgttt cctggctgat aacgccttcg gtgaccaaaa acttgccgag
     4741 cggcagagaa ctgcgttcat ggcgcaataa caacacgtta attgctgaac gattaatatg
     4801 accgagcgtg gtcagtattt cggcgaacag gaactgatgc ggcacatatt gccgccagat
     4861 ttcaccggtc tgctgttccg tgagccactg atgctgaacc gcattgtaca acattgcccg
     4921 cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac cctgtgacaa tttgtccccg
     4981 cagaacaatg acgtaacgca ctttgcgtcc gactttacgc gtcagggccg ccagcgaaac
     5041 cgggtcaata ccatcttcac tgccgacaat taactcatca ttttccagac gcagcggcag
     5101 tactgcataa tgcagcgcta cggaggctgg catttcggca atcagcgagg acgggatctg
     5161 ccaggcatcg atggattccc acgccacgcc gttttgctct gccagcgcct gtgccagctg
     5221 ctcggcgcta atcagcccct gcatcagcat tgaaccgccc aggcgtagac cttcgacgcg
     5281 attacgcagt gctgtatcga gttgttcttc agtgatgacc tgattttcca gcagaatttg
     5341 acctaacggg cgcaacgagc gggtatcgcc agtgacgctg gggaagtcat gcgttgtttt
     5401 atcccacgcg acgcgacgcg gatcgccgtg ctgaagaacc tgtttcagca cgcgccagtt
     5461 agccataaag ttaatcaggt tgccccagaa aagacgcagg acggaaagca ggccctgcgt
     5521 gaggccgtag tagccagtga cgaaaatcac tcgctgcacg atgcggttaa ccatcaagcc
     5581 aaagtttagc cacagcaggg tcattaacca tgcgctgccg ctaaaaatag aaaggaaatg
     5641 ccaggcatcg ggccacaaac tttcatacgc cagcaacagc aaaagctgga tcatcaccag
     5701 catcgcgagg aagctgacaa agttactgat tgcccctttg cggtcgcgcc agagaaagta
     5761 gttcagcgtc aggctggagg tccatttatg ggttttaaag ccctggaaaa caatgccgat
     5821 gatccagcga gatttttgtc gaaccgcagt cgaaaaggta tcggggaaat attcgcgcac
     5881 gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta cgctgctcgc gttctttggc
     5941 ttcgtccacc accggaaaac ggacaaaaat ttccgtcata cctttttctt tcaggcggaa
     6001 gccaatgtcg taatcttcag taagactctg cacgtcgaaa gcaataccgt taccgtcagc
     6061 taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg acgcctgcgc tgggcacttg
     6121 tccggcgagg gcttcacgca ccggaacatc tttgccatgc agttctgaaa actcatcaat
     6181 gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac ggatacactg ggatctgaat
     6241 cagatcttta cgctcgacca gatagttgaa cagacgcaat tccatcggtg aaatcacatc
     6301 ttcggcgtca tgcagaataa aaccagcaaa agcgaaattg gcgctacgtt caaactgggt
     6361 gatggcgtcc agcacgttgt tcaggcagtc ggctttgctg gtgggaccag gacgcgcgca
     6421 gactacctta tgcacgttcg gaaagcgagc gcacacttcg tcaacatcac gttgagtatc
     6481 ggggtcgttg gggtaggtgc caacaaagat atgatagttt tcgtagtcga gcgtggtcgc
     6541 cgccagctcg gccatattgc cgatgacgcc cgtttcattc cacgccggaa ccataatcgc
     6601 taacggtttt tcatctggtt tatacagttc gcggtaactc attcgcgggt agcggcgata
     6661 aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg acatcaataa aaaaatcgtc
     6721 cagcccgctg atgaacatga tgaccgctaa cgttatcgcg attactttta agccgtagag
     6781 ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa ccttacatta acgctggtta
     6841 tgtttagggt ggcgtatatt aaggtttttt atgaattgtg acagcttttt accattaata
     6901 tgtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
     6961 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
     7021 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
     7081 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
     7141 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
     7201 tgacagcgta ctacaattgt ctctctggtt ggcgtataac gaagggcatt agaaagcagg
     7261 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
     7321 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
     7381 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
     7441 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
     7501 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
     7561 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
     7621 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
     7681 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
     7741 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
     7801 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
     7861 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     7921 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     7981 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     8041 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     8101 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     8161 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     8221 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     8281 tgcactttta ctgagtggat tatgatccat gcaaaggcga aaaacgccgc gatggtggcc
     8341 tggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     8401 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     8461 aaagtcgttg tcgattttgc cgcgtagcag cttcaccgcc acatcaatgg cgttagtgtc
     8521 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     8581 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     8641 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     8701 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     8761 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     8821 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     8881 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     8941 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     9001 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     9061 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     9121 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     9181 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     9241 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     9301 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     9361 gttaacgcag cggataacta ccagaacgtg ggctggcgca ccttttttgt tgataatcag
     9421 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     9481 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     9541 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     9601 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     9661 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     9721 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     9781 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     9841 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     9901 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     9961 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
    10021 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
    10081 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
    10141 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
    10201 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt
    10261 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
    10321 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
    10381 ttacgtcaaa gcctggacga tcaaatcagc gcccagcagc gttatctggc gtcgctgcaa
    10441 attaccctgc aacgggcgcg ggcattatat cagcacggcg cggtaagtta tctggaagtg
    10501 ctggatgccg agcgttcttt atttgcaacc cgacaaactt tacttgacct gaattatgcc
    10561 cgtcaggtta acgaaattta tttgtatacc gccttagggg gcggttggca gcaataattt
    10621 ttaactccag gagagaataa atgaaaaaag cactgcaagt cgcaatgttc agtctgttta
    10681 ccgttattgg ctttaatgcc caggctaacg aacatcatca tgaaaccatg agcgaagcac
    10741 aaccacaggt cattagtgcc accggtgtgg taaaaggtgt tgatctggaa agcaaaaaaa
    10801 tcaccatcca tcacgatccg attgctgccg tcaactggcc ggagatgacc atgcgcttta
    10861 ccatcacccc gcagacgaaa atgagtgaaa ttaaaaccgg cgacaaagtg gcgtttaatt
    10921 ttgtccagca gggcaacctt tctttattac aggatattaa agtcagccag taacccaggt
    10981 ttaatgagat gaaaaaaatc gcgcttatta tcggcagcat gatcgcgggc ggtattattt
    11041 cagcggcagg ttttacctgg tttgaccagg cggaaccgca cgcagaaaaa atgccgaccg
    11101 cagaacgtaa aatcttattc tggtacgacc cgatgtatcc caatacgcgg ttcgataaac
    11161 caggtaaatc gccgtttatg gatatggatc tggtgccgaa atatgccgat gaagagagtt
    11221 ctgcgtctgg tgtgcgcatt gacccgactc agacgcagaa tctgggggtg aaaacggcta
    11281 ccgtcacgcg cgggccgctg acttttgccc agagtttccc gacgaatgtc agttacaacg
    11341 agtatcagta tgccattgtg caggctcgcg ctgccgggtt tatcgacaag gtgtatccgc
    11401 ttaccgtggg cgataaagta caaaagggca caccgcttct cgacctgacc attcccgact
    11461 gggtggaagc gcagagtgag tatttactgt tgcgcgaaac cggcggtacg gcgacccaga
    11521 ctgaaggcat tcttgagcgg ctgcgactgg cgggaatgcc ggaggcggat attcgccacc
    11581 tgatcgccac gcaaaaaatc cagactcgct ttacgctcaa agcgcccatt gatggcgtga
    11641 tcaccgcgtt tgatctgcgc gcgggaatga atatcgccaa agataacgtg gtcgcgaaag
    11701 aggaaggtag gttgcttatc tgtgtctgtc attgtctagc attgtccact gtagttaatt
    11761 aactgtctgt tataactcg
//
LOCUS       DAJVPT010000112        11709 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVPT010000112 DAJVPT010000000
VERSION     DAJVPT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..2332)
                     /locus_tag="OQM47_003217"
     CDS             complement(<1..2332)
                     /locus_tag="OQM47_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1481262.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVLRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELV"
     gene            complement(2266..2889)
                     /locus_tag="OQM47_003218"
     CDS             complement(2266..2889)
                     /locus_tag="OQM47_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1481263.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2886..4574)
                     /locus_tag="OQM47_003219"
                     /pseudo
     CDS             complement(2886..4574)
                     /locus_tag="OQM47_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4723..7350)
                     /gene="gyrA"
                     /locus_tag="OQM47_003220"
     CDS             complement(4723..7350)
                     /gene="gyrA"
                     /locus_tag="OQM47_003220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1481264.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7497..8219
                     /locus_tag="OQM47_003221"
     CDS             7497..8219
                     /locus_tag="OQM47_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1481265.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8347..11709)
                     /gene="yfaL"
                     /locus_tag="OQM47_003222"
                     /pseudo
     CDS             complement(8347..11709)
                     /gene="yfaL"
                     /locus_tag="OQM47_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2698 a         3256 c         2906 g         2849 t
ORIGIN      
        1 tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata tcaagctgag
       61 gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg gtatacagca
      121 ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct tcatacaggg
      181 tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc gactgctgtt
      241 ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg aaagtaatca
      301 gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata tctaccgtac
      361 cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg agaattaagc
      421 cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg acggtaaagg
      481 atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga cgcaaccatt
      541 catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggagc acaaccgact
      601 cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg ccgcgttcaa
      661 tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg gagacggtta
      721 acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga ccgctggcgt
      781 cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa cgcaaatcgt
      841 taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca ttttttaccg
      901 gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca gttttgaact
      961 cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt gccacgcgaa
     1021 aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct ccggcaacgg
     1081 cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc gtgacatcga
     1141 cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg gcgctgacga
     1201 tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca tcgacacgat
     1261 cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg tagatttcgc
     1321 tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta cccagaatgt
     1381 atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg gtcaccccgc
     1441 gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc gcttcaccct
     1501 gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta agcgccacgg
     1561 tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc tcgacgaggt
     1621 acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc ggcgagatga
     1681 aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt ttagcctgcc
     1741 atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa aactggttgt
     1801 tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg ggtaatgcct
     1861 gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg cgagatttgc
     1921 cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc agatattgcg
     1981 gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc gggtcaggaa
     2041 tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc cgataatcac
     2101 gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg ctgtcagcaa
     2161 gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc gaatcatcag
     2221 ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc gcgccagaaa
     2281 atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc atcgggtgtc
     2341 cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag ttttcgtggc
     2401 gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct gggcatcgcc
     2461 ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc caataaactg
     2521 gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc cgctcccctg
     2581 attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg gcggcaaata
     2641 ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca gcgtctcgtt
     2701 cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac ttggcccctg
     2761 gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg attgttcgac
     2821 attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg ccagcagccc
     2881 gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc gggttccatc
     2941 tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt cggcattaat
     3001 aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt
     3061 tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac gatgccatca
     3121 gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag ggcgttatta
     3181 accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacacgg
     3241 aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg
     3301 tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt ctggcttacc
     3361 ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc
     3421 cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc
     3481 aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg
     3541 tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc
     3601 gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg
     3661 gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc
     3721 atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa acccagcaac
     3781 ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg ctcttccagg
     3841 ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat cgccgtggct
     3901 tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac cagcatcttg
     3961 tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga
     4021 atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta gctgtcgctg
     4081 gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc
     4141 atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc gggctgatct
     4201 aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac aatgcggcga
     4261 atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac gaaatcttcg
     4321 ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg gctcagagag
     4381 tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt gtgcaccacc
     4441 agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca gacgccagcg
     4501 gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc tttcgccttt
     4561 ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac gggcacagcc
     4621 aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga cttttcaatt
     4681 caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt cttctggctc
     4741 gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac tgccgtcgat
     4801 ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca ctacgttttc
     4861 atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga tgctgatttc
     4921 tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct ggtcgcagtc
     4981 atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga tggagataac
     5041 ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt taccgtaacc
     5101 gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag agacgacttt
     5161 atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca tcgcacggac
     5221 agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta cttcatcttc
     5281 gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga tcgccacttt
     5341 accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag taccgttagc
     5401 ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga tcgcggtgat
     5461 acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg cgccacgagt
     5521 ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg agaagcacag
     5581 aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt ctttaatacg
     5641 tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa gcggctgata
     5701 tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga tcagatcttc
     5761 caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac cgaactgttc
     5821 acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc taccaagaat
     5881 acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca gtttttcgtg
     5941 ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt gctgttcggt
     6001 caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg gacgcgcagc
     6061 atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg gattagcaac
     6121 cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga tgatcgggtc
     6181 aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc gagctttacg
     6241 cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa acgccgcgat
     6301 gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca tgttgatacc
     6361 gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt caccgaccgc
     6421 atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt cacgcagcgc
     6481 gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga tcaggcgcgc
     6541 tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg ttttggcgtc
     6601 aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac ggtaagcttc
     6661 ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc ccgggatgtg
     6721 ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac aaccgttgat
     6781 gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga taccggaaga
     6841 accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt tttccgtgcc
     6901 gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca gttcatgggc
     6961 aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc cgtcgatgga
     7021 accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct gcgccatacg
     7081 gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga ttacgtcacc
     7141 aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta cgttcatggc
     7201 gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca gcgcacggcc
     7261 aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct caatgttgac
     7321 cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact gtatcccgga
     7381 ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat acgctttatt
     7441 cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga
     7501 atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct aaatttgaag
     7561 ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac cgcattaacc
     7621 cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg
     7681 atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc gcgacggtga
     7741 ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg ctggaaagcg
     7801 gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa catgccgggc
     7861 agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg cagtcagtgg
     7921 tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg acgcttaacc
     7981 gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg cgcatggtgc
     8041 ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt ggctgggtgg
     8101 atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg atcactaatt
     8161 cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag aataagtgag
     8221 gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat caggcctaca
     8281 aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt gatggtcaga
     8341 cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact
     8401 ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat tgcccgtgac
     8461 tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc tgccatcttc
     8521 ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg gtgtgacacc
     8581 aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat ccccctgcga
     8641 ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc cctgataaat
     8701 cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat acccagcttc
     8761 cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat gttcggaaac
     8821 atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct gcttctgatt
     8881 accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct ggttatcggc
     8941 gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc caccaaccgc
     9001 cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc cgctaagctg
     9061 caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat gataacgtcc
     9121 gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc gtcgctccat
     9181 cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt tggcattcag
     9241 caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat ccggcgtggg
     9301 atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt cttgcgatcg
     9361 cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca tgttgacgta
     9421 gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat cagctgcgaa
     9481 atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg agttaaccac
     9541 cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta ccgagtcatc
     9601 gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac cgtttaccgt
     9661 cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat tggtcagatc
     9721 accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt tggtattaag
     9781 ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc cgttaaatag
     9841 tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg taccatcgct
     9901 gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat ccgcgccagt
     9961 aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga tatccgcaac
    10021 atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac cgctggcggt
    10081 cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct gatgctgccc
    10141 gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa ctgccacttc
    10201 accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc gagtatcgac
    10261 attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa cctggcggtt
    10321 atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg aggcgatttg
    10381 tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc tcgcatcgac
    10441 caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc cacctgcaag
    10501 atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt caaggccatc
    10561 attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca caccgccgtt
    10621 taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt cgccttccag
    10681 cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg actgcgcccc
    10741 tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt cgatgatgcc
    10801 agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta aagtgccatt
    10861 ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt ttagctcagc
    10921 ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt gcggatcgtc
    10981 ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg taacttcacc
    11041 gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta ccagatcgcc
    11101 ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc cgtcattctc
    11161 tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt cacttaagcc
    11221 gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg ctgcgctatt
    11281 gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag aaatatcaat
    11341 aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt cggctgtgtt
    11401 atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc tgtcgctatt
    11461 attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg atgtatatgc
    11521 ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg tataaattgc
    11581 gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta aatcgacggc
    11641 accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg cgacgttacc
    11701 ggaaaaaat
//
LOCUS       DAJVPT010000113        11664 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVPT010000113 DAJVPT010000000
VERSION     DAJVPT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..338
                     /locus_tag="OQM47_003223"
     CDS             <1..338
                     /locus_tag="OQM47_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1481266.1"
                     /translation="MCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQL
                     RKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLL
                     LRATGGVCV"
     gene            complement(455..730)
                     /gene="yjfN"
                     /locus_tag="OQM47_003224"
     CDS             complement(455..730)
                     /gene="yjfN"
                     /locus_tag="OQM47_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1481267.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(879..1208)
                     /gene="bsmA"
                     /locus_tag="OQM47_003225"
     CDS             complement(879..1208)
                     /gene="bsmA"
                     /locus_tag="OQM47_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1481268.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1390..2139
                     /gene="yjfP"
                     /locus_tag="OQM47_003226"
     CDS             1390..2139
                     /gene="yjfP"
                     /locus_tag="OQM47_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1481269.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2136..2891)
                     /gene="ulaR"
                     /locus_tag="OQM47_003227"
     CDS             complement(2136..2891)
                     /gene="ulaR"
                     /locus_tag="OQM47_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1481270.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2999..4063)
                     /gene="ulaG"
                     /locus_tag="OQM47_003228"
     CDS             complement(2999..4063)
                     /gene="ulaG"
                     /locus_tag="OQM47_003228"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1481271.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4418..5815
                     /gene="ulaA"
                     /locus_tag="OQM47_003229"
     CDS             4418..5815
                     /gene="ulaA"
                     /locus_tag="OQM47_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1481272.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5831..6136
                     /gene="ulaB"
                     /locus_tag="OQM47_003230"
     CDS             5831..6136
                     /gene="ulaB"
                     /locus_tag="OQM47_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1481273.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6146..6610
                     /gene="ulaC"
                     /locus_tag="OQM47_003231"
     CDS             6146..6610
                     /gene="ulaC"
                     /locus_tag="OQM47_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1481274.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6624..7274
                     /gene="ulaD"
                     /locus_tag="OQM47_003232"
     CDS             6624..7274
                     /gene="ulaD"
                     /locus_tag="OQM47_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1481275.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7284..8138
                     /gene="ulaE"
                     /locus_tag="OQM47_003233"
     CDS             7284..8138
                     /gene="ulaE"
                     /locus_tag="OQM47_003233"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1481276.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8138..8824
                     /gene="ulaF"
                     /locus_tag="OQM47_003234"
     CDS             8138..8824
                     /gene="ulaF"
                     /locus_tag="OQM47_003234"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1481277.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8953..9228)
                     /locus_tag="OQM47_003235"
     CDS             complement(8953..9228)
                     /locus_tag="OQM47_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1481278.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9555..9950
                     /gene="rpsF"
                     /locus_tag="OQM47_003236"
     CDS             9555..9950
                     /gene="rpsF"
                     /locus_tag="OQM47_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1481279.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9957..10271
                     /gene="priB"
                     /locus_tag="OQM47_003237"
     CDS             9957..10271
                     /gene="priB"
                     /locus_tag="OQM47_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1481280.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10276..10503
                     /gene="rpsR"
                     /locus_tag="OQM47_003238"
     CDS             10276..10503
                     /gene="rpsR"
                     /locus_tag="OQM47_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1481281.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10545..10994
                     /gene="rplI"
                     /locus_tag="OQM47_003239"
     CDS             10545..10994
                     /gene="rplI"
                     /locus_tag="OQM47_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1481282.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11065..11628)
                     /locus_tag="OQM47_003240"
                     /pseudo
     CDS             complement(11065..11628)
                     /locus_tag="OQM47_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2761 a         2870 c         3082 g         2951 t
ORIGIN      
        1 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
       61 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
      121 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
      181 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
      241 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
      301 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
      361 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
      421 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
      481 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
      541 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
      601 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
      661 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
      721 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
      781 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
      841 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
      901 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
      961 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     1021 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     1081 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     1141 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     1201 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     1261 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     1321 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     1381 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     1441 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     1501 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     1561 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     1621 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     1681 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     1741 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     1801 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     1861 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     1921 acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     1981 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     2041 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     2101 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     2161 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     2221 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     2281 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     2341 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     2401 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     2461 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     2521 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     2581 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     2641 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     2701 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     2761 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     2821 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     2881 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     2941 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     3001 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     3061 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     3121 agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac agcacgcgga
     3181 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     3241 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     3301 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     3361 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     3421 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     3481 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     3541 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     3601 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcatcagcac
     3661 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     3721 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     3781 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     3841 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     3901 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     3961 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     4021 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     4081 atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact attccttcag
     4141 gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgggg
     4201 aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa taattgtttc
     4261 gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca catttaatca
     4321 taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca ttccgggtgt
     4381 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     4441 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     4501 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     4561 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     4621 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     4681 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     4741 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     4801 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     4861 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     4921 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     4981 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     5041 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     5101 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     5161 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     5221 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     5281 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     5341 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     5401 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     5461 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     5521 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     5581 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     5641 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     5701 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     5761 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     5821 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     5881 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     5941 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     6001 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     6061 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     6121 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     6181 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     6241 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     6301 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     6361 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     6421 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg atgccaacac
     6481 gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     6541 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
     6601 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
     6661 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
     6721 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
     6781 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
     6841 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
     6901 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
     6961 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
     7021 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
     7081 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
     7141 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
     7201 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
     7261 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
     7321 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
     7381 tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc ctcgactgga
     7441 gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg cgtgtgccgt
     7501 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
     7561 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
     7621 tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa acgcgtcgtc
     7681 gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag gtgacgctgg
     7741 cgatggagat catggattat ccgttgatga actccatcag caaggcgctg ggttacgcgc
     7801 actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg tcggcgtggg
     7861 acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga
     7921 aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg
     7981 aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg attgagatgt
     8041 ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat tgggtgaaag
     8101 cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa aacagcaggt
     8161 gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt ggggcaacgt
     8221 cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg ttgcctacga
     8281 aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg tggaagggga
     8341 gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt acccgtcgct
     8401 tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg cggggctggc
     8461 gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc cgtgtacgcg
     8521 tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca aagtgattat
     8581 cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt atcagcacgg
     8641 accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag tgatggaaga
     8701 agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc atatcgacag
     8761 ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt acgggcagaa
     8821 gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa ttccgcagcc
     8881 gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg
     8941 ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg
     9001 ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt cttcatttaa
     9061 tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca cgcctaagct
     9121 ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg cccatgtatt
     9181 tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag atacctcctc
     9241 gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt taatcaatga
     9301 taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact gtgacggaat
     9361 gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat ttatttctgc
     9421 tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt cagccgacct
     9481 ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct gaataatccg
     9541 taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct gaccagagcg
     9601 aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca gaaggcaaga
     9661 tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac aaactgcaca
     9721 aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat gagctggaaa
     9781 ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc aagcacgctg
     9841 ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt cgcgatgatt
     9901 tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa tttctgatga
     9961 ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag gtcagtccat
    10021 caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag gaagccggct
    10081 ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa aaccaggcca
    10141 ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt tcatgccaca
    10201 aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa ttgatagatt
    10261 ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg tttcaccgcg
    10321 gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta catcaccgaa
    10381 agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca gcgtcagctg
    10441 gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga tcgccatcag
    10501 taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa gttattctgc
    10561 ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa gcgggctatg
    10621 ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa aacattgaat
    10681 tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg gcagctgcta
    10741 atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct aaagctggcg
    10801 acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct gtaactgcag
    10861 ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg cgtaccactg
    10921 gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg atcgtaaacg
    10981 tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt ggtcggcgtt
    11041 ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac cattctcttg
    11101 tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc ttatgtctcg
    11161 gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat ttgataagga
    11221 acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca atgcagcaat
    11281 gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga tataaagatc
    11341 gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg ttacaacgta
    11401 tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa caacctcgtg
    11461 tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat atttcagttc
    11521 tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa tattatctaa
    11581 tgtagcttca tggttccatc caaaagaatt atcaccattc attgttactg aatcgccacg
    11641 ggtttaacag acacctcaga gtca
//
LOCUS       DAJVPT010000114        11427 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVPT010000114 DAJVPT010000000
VERSION     DAJVPT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1554
                     /gene="trg"
                     /locus_tag="OQM47_003241"
     CDS             <1..1554
                     /gene="trg"
                     /locus_tag="OQM47_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1481283.1"
                     /translation="VLFALSSALAGYFLWQADRDQRDVTAEIEIRTGLANSSDFLRSA
                     RINMIQAGAASRIAEMEAMKRNIAQAESEIKQSQQGYRAYQNRPVKTPADEAFDTELN
                     QRFQAYITGMQPMLKYAKNGMFEAIINHESEQIRPLDNAYTDILNKAVKIRSTRANQL
                     AELAHQRTRLGGMFMIGAFVLALVMTLITFMVLRRIVIRPLQHAAQRIEKIASGDLTM
                     NDEPAGRNEIGRLSRHLQQMQHSLGMTVGTVRQGAEEIYRGTSEISAGNADLSSRTEE
                     QAAAIEQTAASMEQLTATVKQNADNAHHASKLAQEASIKASDGGQTVSGVVKTMGAIS
                     TSSKKISEITAVINSIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLASRSAQA
                     AKEIEGLISESVRLIDLGSDEVATAGKTMSTIVDAVASVTHIMQEIAAASDEQSRGIT
                     QVSQAISEMDKVTQQNASLVEEASAAAVSLEEQAARLTEAVNVFRLNKHSVSAEPRGA
                     GEPVSFATV"
     gene            complement(1592..2515)
                     /locus_tag="OQM47_003242"
     CDS             complement(1592..2515)
                     /locus_tag="OQM47_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1481284.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            2732..4075
                     /locus_tag="OQM47_003243"
     CDS             2732..4075
                     /locus_tag="OQM47_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1481285.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4300..5955
                     /gene="opgD"
                     /locus_tag="OQM47_003244"
                     /gene_synonym="mdoD"
     CDS             4300..5955
                     /gene="opgD"
                     /locus_tag="OQM47_003244"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1481286.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6095..6319
                     /locus_tag="OQM47_003245"
     CDS             6095..6319
                     /locus_tag="OQM47_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1481287.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6382..6918
                     /gene="rimL"
                     /locus_tag="OQM47_003246"
     CDS             6382..6918
                     /gene="rimL"
                     /locus_tag="OQM47_003246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1481288.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(6913..7893)
                     /locus_tag="OQM47_003247"
     CDS             complement(6913..7893)
                     /locus_tag="OQM47_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481289.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8017..9009
                     /gene="tehA"
                     /locus_tag="OQM47_003248"
     CDS             8017..9009
                     /gene="tehA"
                     /locus_tag="OQM47_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1481290.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9006..9599
                     /gene="tehB"
                     /locus_tag="OQM47_003249"
     CDS             9006..9599
                     /gene="tehB"
                     /locus_tag="OQM47_003249"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1481291.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDLDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            9902..10558
                     /locus_tag="OQM47_003250"
     CDS             9902..10558
                     /locus_tag="OQM47_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1481292.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10576..11082)
                     /gene="tnpA"
                     /locus_tag="OQM47_003251"
                     /pseudo
     CDS             complement(10576..11082)
                     /gene="tnpA"
                     /locus_tag="OQM47_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11090..11407
                     /locus_tag="OQM47_003252"
                     /pseudo
     CDS             11090..11407
                     /locus_tag="OQM47_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2964 a         2676 c         2880 g         2907 t
ORIGIN      
        1 gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc cgatcgcgat
       61 cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag ttcagatttt
      121 ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat tgcggaaatg
      181 gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc gcagcaaggt
      241 tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt cgacactgaa
      301 ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa atatgccaaa
      361 aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc gctggataat
      421 gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc caaccaactg
      481 gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg cgcgtttgtg
      541 cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt cattcgtcca
      601 ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac gatgaatgat
      661 gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca aatgcagcat
      721 tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta tcgtggcacc
      781 agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca agcggcggct
      841 atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca gaatgcggat
      901 aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag cgatggcggg
      961 cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc gaagaaaatt
     1021 tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct ggcactgaat
     1081 gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt tgtcgccagc
     1141 gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga aggcttgatc
     1201 agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc agggaaaacc
     1261 atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga aatcgccgcc
     1321 gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc tgaaatggat
     1381 aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc ggtgtccctt
     1441 gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa caaacattct
     1501 gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt gtgaaaatgt
     1561 tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt cgaatagcgt
     1621 taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt attccaatcg
     1681 gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga cgcaggtcgt
     1741 cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta agttgacgag
     1801 atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc tggctctgca
     1861 ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg accggccatt
     1921 ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg cggacaacca
     1981 gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt tcaggatctg
     2041 acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga atcatagggt
     2101 tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac tgacctataa
     2161 ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg acatcattat
     2221 taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca agaactctga
     2281 ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga cgaccacgct
     2341 caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag agcgcaggtt
     2401 gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg acagctacga
     2461 atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt tccataagcg
     2521 atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga taacttgatt
     2581 gcaaaatatt attaacaatt aaagcaatta tgttacagca aaatggataa tattgatgtt
     2641 ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg ttacctacac
     2701 tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg cggatgagat
     2761 tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc cgcaatatgg
     2821 cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca atcctcaact
     2881 gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac gtcatggggc
     2941 gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg ccattatggg
     3001 gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac attcgacagc
     3061 atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt ttacatcctt
     3121 actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg agattctgcg
     3181 tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg agcagcaggg
     3241 cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg
     3301 gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt
     3361 gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc cacgtacgct
     3421 ggatattgac cgggtgcagt cgatgatgcc tgaatgcgga attgaaccca aaattctcat
     3481 cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct ttaaagcact
     3541 ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc gctttggtga
     3601 aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg atgatcttct
     3661 gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac aggaaacctt
     3721 ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat ggttccggta
     3781 ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag acgatccaca
     3841 gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag atttcttgcc
     3901 cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga cacgcagtca
     3961 cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt tggatgagtt
     4021 ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc tttaaattct
     4081 gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct catcccatcg
     4141 acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga ttattaataa
     4201 atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa acgaccagcg
     4261 cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag acgatttatt
     4321 aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct
     4381 caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg ttttgacttc
     4441 tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg tgcgcctcgt
     4501 ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat tcaatacgac
     4561 gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca gttcttccat
     4621 atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc aacacatctg
     4681 gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg tgttgatacc
     4741 aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt taaagccccc
     4801 gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg cgccgttgat
     4861 gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta caccgacagt
     4921 aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc gggggcaact
     4981 acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta taagttcact
     5041 atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa
     5101 gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg tactaatgaa
     5161 cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct gtccatgtgg
     5221 cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt gcagttcaat
     5281 gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg tgacttctcc
     5341 cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt
     5401 aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg cgaaacgctg
     5461 gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga tgagtttgca
     5521 ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt
     5581 atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg tgaacactat
     5641 cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc
     5701 gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa
     5761 attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta tccgacttcg
     5821 gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga cgctatcagt
     5881 gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta tgttgacgac
     5941 cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc gtctggttaa
     6001 acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt gtgtatttca
     6061 gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc gagatttaat
     6121 atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac acaataaact
     6181 tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg cggaagtggt
     6241 ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc tgcagcagga
     6301 gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt ttaacaacag
     6361 catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc acttgaatta
     6421 catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa aaataaaacc
     6481 tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga cacgcgaaaa
     6541 acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt catgattttc
     6601 aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact gaataaaact
     6661 gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat ttctcaggcg
     6721 ctgcagacat tgattcatca ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa
     6781 tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt tatccttgaa
     6841 ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa cttatacgcg
     6901 cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct cctcgccgtt
     6961 aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa cgacggcgtg
     7021 gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc tctcaccttg
     7081 tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat ttccgccaat
     7141 caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg ccacctgcga
     7201 actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct gcgcgtgacc
     7261 gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt caacactggc
     7321 aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg catcgccata
     7381 aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat aaatttggag
     7441 caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat gttgatcaat
     7501 taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa tggtgacact
     7561 gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt tatccgttgc
     7621 gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg aaatcaccgt
     7681 tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct gtgctaacac
     7741 cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa aatcgctaat
     7801 ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat aactaaaggc
     7861 tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt gacttactat
     7921 aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa atgcatttca
     7981 aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa agtgctcaat
     8041 ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt tgcctggcgc
     8101 tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt gattctggcg
     8161 atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg ctttccgcat
     8221 agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt gtttccggca
     8281 acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc ggtgtgcatg
     8341 ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc gggattatgg
     8401 cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac agttgccaac
     8461 aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt
     8521 ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca gcgtctgcgc
     8581 agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct cgctcctgcg
     8641 ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac gctggcgaaa
     8701 atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat gccatggtat
     8761 ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc tgcactggca
     8821 accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac gctggcggtg
     8881 ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac ttttgcgctt
     8941 ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa agcagaggac
     9001 aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta acccgcacac
     9061 actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct
     9121 gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatctt gacgcatggg
     9181 ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa aatctggata
     9241 atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag tacgatttta
     9301 ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg ttgattgcca
     9361 atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg atggataccg
     9421 ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa ttacgtcgat
     9481 attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg caccgcaccg
     9541 acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt aaaaaatgac
     9601 ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg ttcttctttt
     9661 cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg aagtttttta
     9721 gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt ttcggcattg
     9781 actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt tttgccacaa
     9841 cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca ggaaactatt
     9901 catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg
     9961 ttgtgcctct aaaatcaccc agccagataa atataacaat tactctgatt taaaagaaac
    10021 aacctcagca acaggtaaac ctgttttacg ttgggtagac ccgagttttg atcaaagcaa
    10081 atatgacagc atcgtctgga acccaatcac ttattatccg gtaccaaaac cttctaccca
    10141 ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa tgaaagaagc
    10201 gatagcgcag cgtaaaccag tggttaccac cgctgggccg cgtagtctga ttttccgtgg
    10261 ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag tggttcctgt
    10321 tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg atactcgcct
    10381 ctattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca aagtcgttcg
    10441 tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg aaaatattaa
    10501 acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttaaca aaaagtagtt
    10561 ccagacgcgc catcgttaga tggcgctttt tatccggtgc gccgtaaaac cccatccttc
    10621 agggcgggga tataaggcgc ggttttccac ctgaccaggt gtttgctgtt gttcaatgta
    10681 ttggcggatg acggatattg gcgcacctcc gcagctactg gcaaaatagc caggactcca
    10741 caaaacgcct ttgtagtaat acctgactgc aatatctggt cgatctcgtc gcagtaacct
    10801 acccgatacg cctttgaggc tgtttaccag actggatatc gccagtttgg gaggttagtt
    10861 aattaacaaa tggacgtgat ctggttcgcc atccatttca accagttcag cttcaaaata
    10921 agcacataca tttgaaaagt aagtgcgtag tttttctgtc gcgtcatggt caaaaatctg
    10981 gcgtcggtat ctggtgacaa agactaggtg aacatgcatc aggaaaacac aatgccttcc
    11041 acgccggata tcggtttctt ttttcacaga ccaaagtatc atttgacctg tgaaacgatt
    11101 acaggcattt aaattccagt taagaccagg tggtcaacag gagtgtgaaa tgaggcgctt
    11161 cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggca cgtcagaatg agaatcatga
    11221 ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggctggttg agtggaaaaa
    11281 tgccactgaa acgcaatggc ttaaagattc tccctcacag ccattgcaac agtcactgaa
    11341 agaccttgag cgggcttaca aaaacttctt ccggaagcgg gctgcttttc cccgattcaa
    11401 aaagcggtga cagaatgatg cattccg
//
LOCUS       DAJVPT010000115        11413 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVPT010000115 DAJVPT010000000
VERSION     DAJVPT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..828
                     /locus_tag="OQM47_003253"
     CDS             <1..828
                     /locus_tag="OQM47_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1481293.1"
                     /translation="KSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKH
                     KLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFV
                     LTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDN
                     GNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITR
                     RMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(1031..1384)
                     /locus_tag="OQM47_003254"
     CDS             complement(1031..1384)
                     /locus_tag="OQM47_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1481294.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1522..3168)
                     /gene="groL"
                     /locus_tag="OQM47_003255"
     CDS             complement(1522..3168)
                     /gene="groL"
                     /locus_tag="OQM47_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1481295.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3212..3505)
                     /locus_tag="OQM47_003256"
     CDS             complement(3212..3505)
                     /locus_tag="OQM47_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1481296.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3781..5037
                     /gene="yjeH"
                     /locus_tag="OQM47_003257"
     CDS             3781..5037
                     /gene="yjeH"
                     /locus_tag="OQM47_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1481297.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5053..5529)
                     /gene="fxsA"
                     /locus_tag="OQM47_003258"
     CDS             complement(5053..5529)
                     /gene="fxsA"
                     /locus_tag="OQM47_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1481298.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5866..7302
                     /gene="aspA"
                     /locus_tag="OQM47_003259"
     CDS             5866..7302
                     /gene="aspA"
                     /locus_tag="OQM47_003259"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1481299.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7420..8721
                     /gene="dcuA"
                     /locus_tag="OQM47_003260"
     CDS             7420..8721
                     /gene="dcuA"
                     /locus_tag="OQM47_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1481300.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8837..9175
                     /gene="cutA"
                     /locus_tag="OQM47_003261"
     CDS             8837..9175
                     /gene="cutA"
                     /locus_tag="OQM47_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1481301.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9151..10848
                     /gene="dsbD"
                     /locus_tag="OQM47_003262"
     CDS             9151..10848
                     /gene="dsbD"
                     /locus_tag="OQM47_003262"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1481302.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10885..>11413
                     /locus_tag="OQM47_003263"
     CDS             10885..>11413
                     /locus_tag="OQM47_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1481303.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAE"
BASE COUNT         2724 a         2969 c         2817 g         2903 t
ORIGIN      
        1 aagagtaata atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg
       61 ttgttcttta tgtctgtcat ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg
      121 catcaaaagc ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa
      181 gtcattaaga aaatggcaga aaacatccca gaaattctgg ttgatgagct taaaaatgcc
      241 gatattaatc gtcgggttaa cacgctggaa cttaccgata atcaaggcga aaatcttacc
      301 ttcgtattaa cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt
      361 gaaatgctgg cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca
      421 ttacgtatta cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggacaat
      481 ggcaatctgg aatatgacac ctattcccaa ccggaatgga aacataactt gttcgaccat
      541 tatctggcgg tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca
      601 gtggtgaaaa cgcgtgaagc aacgccgggt aaagaaatag aggcgatcac tcgccgaatg
      661 cttgatttca gcccacgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc
      721 accgtagcgg cgaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat
      781 cacttacgtg tacaatccac cagcaaaact gcaccacagg cgaaataatc tcatctttgc
      841 taatgcgagg ctggagccat ccagcctgaa atgactgata acgtgatcgt tggtcatgcc
      901 ggatgcggcg taaacgcctt atccggccta caaattctca agcattcaat cagttgcaat
      961 gcctgctgta ggcctgataa gcgaagcgca tcaggcaata atacgtttgt cacccagcct
     1021 ctggatcaca tcagttcggg cacttataaa cctgaccgat aatctggcta tccgtcggga
     1081 caaaactgga caacattccc tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca
     1141 ttgcagccgc ctggttgcgc agatcgtttg ctgcgccgcg catagaaccg ccctcttctc
     1201 cgtgttgccc ggaaagccag ttgctttgct tacctgtcgc agtaccaatc agctggcact
     1261 ctgcgcctgg ctgctcgtcc acaatgcgta cactctgacc ggcagcactc aattcgttgc
     1321 tggagctaca acccgccatc agtagcgcgg caccgacaat ccctgctaag tattttacgt
     1381 gcatgttatt ccccataatc aatgagctgg acgccactcg cgtcatccgt gtctgaatct
     1441 tatactagaa agatgaccaa aagaaaaacc cccagacatt tctgcccggg ggtttgttta
     1501 tttctgcgaa gtgcagggca attacatcat gccgcccatg ccacccatgc cgcccatacc
     1561 gccagcagcg cctaagtcag ctgcatcgtt tttcggcagg tcggtaacca tgcattcggt
     1621 ggtgatcatc aggccagcca cagaagctgc gtactgcaga gcagaacggg ttactttggt
     1681 tgggtccagg atacccatgt cgatcatgtt gccgtattct tcggttgctg cgttgtaacc
     1741 gtagttgccg tcgccgcctt taacggtgtt agcaacaaca gacggttctt cgccgcagtt
     1801 caggacgatc tgacgcagcg gagcttccat tgcacgcagt gcaactttga tacccacgtt
     1861 ctggtcttcg ttctgaccac gcaggtcagc cagtttagac gctacgcgga tcagcgcaac
     1921 accaccacca gcaaccacgc cttcttctac cgcagcacgg gtcgcgtgca gggcatcttc
     1981 aacgcgtgct tttttctctt tcatttcaac ttcggtagca gcacctactt tgataactgc
     2041 aacgccgcct gccagtttcg ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt
     2101 tgcttcttca atctgctgac ggatctgagc aacacggccc tggattgcag cttcttcacc
     2161 cacgccatcg atgatagtgg tggtgtcttt gttgatcaca acacgtttag cctgacccag
     2221 gtcttccagg gttgcttttt ccagctccat accgatctct tcagagatta cggtaccgcc
     2281 agtcagggtt gcgatatcct gcagcatagc tttacgacga tcgccgaagc ccggagcttt
     2341 aaccgcagca actttcacga tgccacgcat ggtgttaaca accagagttg ctagcgcttc
     2401 gccttcaaca tcttcagcga tgatcagcag cggtttgcct gctttggcaa cagcttccag
     2461 aaccggcagc atttcgcgga tgttggagat tttcttgtca gccagcagga tgaacgggct
     2521 ttccagttct actgcgccag tttccggctt gttgatgaag taaggagaca ggtagccacg
     2581 gtcgaactgc ataccttcaa ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac
     2641 ggtgataacg ccttctttac cgactttgtc catcgcttca gcgatcagtt tacctacggt
     2701 ttcgtcggag ttagcggaga tggtaccaac ctgagcaatc gctttagaat cagagcacgg
     2761 tacggacagc gctttcagtt cttcaactgc agcggtaacc gctttgtcga taccacgttt
     2821 caggtccatc gggttcatgc ccgcagcaac agctttcaga ccttcagtga tgatagcctg
     2881 agccagtacg gttgcagtgg tggtaccgtc gcctgcagcg tcgttcgctt tggaggcaac
     2941 ttctttcacc atctgcgcac ccatattttc gaacttgtct tccagttcga tttcacgagc
     3001 aacggaaaca ccatctttgg tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt
     3061 acggcctttt ggaccgaggg taactttcac tgcatctgcc agtacgttta cgccgcgcag
     3121 cattttcaca cgagcgtcgt taccgaattt tacgtcttta gctgccatta tctttattcc
     3181 ttaaattcgt atgttcagtg tcgtgcgcgg attacgcttc aacaattgcc agaatgtcgc
     3241 tttcggacat gatcaacact tcttcattgt cgatcttctc ggatttcaca ccgtagccat
     3301 cgttgaaaat aacgatgtcg ccaactttca cgtccagcgg cttcacttcg ccattttcaa
     3361 ggatacggcc attgccgaca gccagcactt cgccgcgggt ggatttagcc gctgcagagc
     3421 cggtcagaac gatgccgcca gcagatttag tttcaacttc tttacgcttg acgatcacgc
     3481 gatcatgcaa tggacgaata ttcattgata actctccttt gagaaagtcc gtatctgtta
     3541 tgggtgacgc cggagcttac gtggtttccc ggctggtgac cagagaaatg gggatgaggc
     3601 ttcgcccctt caagggggaa aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa
     3661 ccctgattca cctcacattt catcgcaatt tcttcatcgc cgtcataagc gaatctgatt
     3721 gtgctaccat cgaaaatcta cgcagttgcc caaaatttgg gcgcaatcgg atatcaaccc
     3781 atgagtggac tcaaacaaga actggggctg gcccagggca ttggcctgct atcgacgtca
     3841 ttattaggca ctggcgtgtt tgccgttcct gcgttagctg cactggtagc aggcaataac
     3901 agcctgtggg cgtggcccgt tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg
     3961 attctgggtc gccactatcc cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt
     4021 ggttcgcggc ttgagcgagt caccggctgg ctgtttttat cggtcattcc cgtgggtttg
     4081 cctgccgcac tacaaattgc cgccggattc ggccaagcaa tgtttggctg gcatagctgg
     4141 caactgttgt tggcagaact cggtacgctg gcgttggtgt ggtatatcgg tactctcggt
     4201 gccagttcca gtgctaatct acaaaccgtt attgccggac ttatcgtcgc actgattgtc
     4261 gctatctggt gggcgggcga tatcaaacct gcgagtatcc ccttccccgc gccaggaaat
     4321 atcgaactta ccgggttatt tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg
     4381 gaagcatttg cccatcttgc ctcggaattt aaaaatccag agcgtgattt tcctcgtgct
     4441 ttgatgattg gtctgctgct ggcaggatta gtctactggg gctgtacggt agtcgtctta
     4501 cacttcgacg cctatggtga acaaatggcg gcggcagcat cgcttccaaa aattgtagta
     4561 cagttgttcg gtgtaggagc gttatggatt gcctgcgtaa ttggctatct ggcctgcttt
     4621 gccagtctta acatttatat acagagcttc gcccgcctgg tctggtcgca ggcgcaacat
     4681 aatcctgacc actacctggc acgcctctct tctcgccata tcccgaataa tgccctcaat
     4741 gcggtgcttg gctgctgcgt ggtgagcacg ttggtaattc atgccttaga gatcaatctg
     4801 gacgctctta ttatttatgc caatggcatc tttattatga tttatctgtt atgcatgctg
     4861 gcaggctgta aattattgca aggtcgttat cgactactgg cggtggttgg cgggctgtta
     4921 tgcgttctgt tactggcaat ggtcggctgg aaaagtctct atgcgctgat catgctggtg
     4981 gggttatggc tgtttctgcc aaaacgaaaa acgccggaaa atggcataac cacataatcc
     5041 ggcgtttcga cattaatcct ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc
     5101 ctttcgctga tactcaccat caaaagtatt accgccaccg gtcccggcgc taaaaccgcc
     5161 gcctggcatg cgagaaaagc gcaaatgtgg catcaacttc actgtcagat gcttttgcac
     5221 cggcggcaat aaaagcagaa gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag
     5281 caaaccggca ataatcagcg aaacactttt aatcatctcc gccgcagggt tttcacccgc
     5341 cgccattttt tgctgcatca gcacgaaatt cttaaagccc tggttacgta ccagtgacat
     5401 accgataact gacgtgaata taaccagcac gagggtcagc aataccccca atacatgggc
     5461 aacctgaata aagattgaaa tctcaatata gacataaagg aaaatggcaa taaaaggtaa
     5521 ccagcgcaaa ggtttctcct gtaatagcag ccggttaaac ccggctacct gaatgggttg
     5581 cgaatcgcct tcagcttata ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta
     5641 tttttggtag taatcccaaa gcggtgatct atttcacaaa ttaataatta aggggtaaaa
     5701 accgacactt aaagtgatcc agattacggt agaaatcctc aagcagcata tgatctcggg
     5761 tattcggtcg atgcagggga taatcgtcgg tcgaaaaaca ttcgaaacca catatattct
     5821 gtgtgtttaa agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt
     5881 cgtatcgaag aagatctgtt gggtaccagg gaagttccag ctgatgccta ctatggtgtt
     5941 cacactctga gagcgattga aaacttctat atcagcaaca acaaaatcag tgatattcct
     6001 gaatttgttc gcggtatggt aatggttaaa aaagccgcag ctatggcaaa caaagagctg
     6061 caaaccattc ctaaaagtgt agcgaatgcc atcattgccg catgtgatga agtcctgaac
     6121 aacggaaaat gcatggatca gttcccggta gacgtctacc agggcggcgc aggtacttcc
     6181 gtaaacatga acaccaacga agtgctggcc aatatcggtc tggaactgat gggtcaccag
     6241 aaaggtgaat atcagtacct gaacccgaac gaccatgtta acaaatgtca gtccactaac
     6301 gacgcctacc cgaccggttt ccgtatcgca gtttactctt ccctgattaa gctggtagat
     6361 gcgattaacc aactgcgtga aggctttgaa cgtaaagctg tcgaattcca ggacatcctg
     6421 aaaatgggtc gtacccagct gcaggacgca gtaccgatga ccctcggtca ggaattccgc
     6481 gctttcagca tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg
     6541 ctggaagtta accttggcgc aacagcaatc ggtactggtc tgaacacgcc gaaagagtac
     6601 tctccgctgg cagtgaaaaa actggctgaa gtcactggct tcccatgcgt accggctgaa
     6661 gacctgatcg aagcgacctc tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa
     6721 cgcctggctg tgaagatgtc caaaatctgt aacgacctgc gcttgctctc ttctggtcca
     6781 cgtgccggcc tgaacgagat caacctgccg gaactgcagg cgggctcttc catcatgcca
     6841 gctaaagtaa acccggttgt tccggaagtg gttaaccagg tatgcttcaa agtcatcggt
     6901 aacgacacca ctgttaccat ggcagcagaa gcaggtcagc tgcagttgaa cgttatggaa
     6961 ccggtcattg gccaggccat gttcgaatcc gttcacattc tgaccaacgc ttgctacaac
     7021 ctgctggaaa aatgcattaa cggcatcact gctaacaaag aagtgtgcga aggttacgtt
     7081 tacaactcta tcggtatcgt tacttacctg aacccgttca tcggtcacca caacggtgac
     7141 atcgtgggta aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc
     7201 ggtctgttga ctgaagcgga acttgacgat attttctccg tacagaatct gatgcacccg
     7261 gcttacaaag caaaacgcta tactgatgaa agcgaacagt aatcgtacag ggtagtacaa
     7321 ataaaaaagg cacgtcagat gacgtgcctt ttttcttgtg agcagtaact taaaaataac
     7381 aatctaatat caacttgtta aaaaacaagg aaggctaata tgctagttgt agagctcatc
     7441 atagttttgc tggcgatctt cttgggcgcc agattggggg gaataggtat tggttttgca
     7501 ggcggattgg gggtgctggt tcttgccgct attggcgtta aacccggtaa catcccgttc
     7561 gatgtcattt ccattatcat ggcggttatc gccgctattt ctgccatgca ggttgctggc
     7621 ggtctggact atctggttca tcagacagaa aagctgctgc gccgtaaccc gaaatacatc
     7681 acgatcctcg caccgatcgt gacctatttc ctgactatct ttgctggtac tggcaacatc
     7741 tctctggcga cactgccagt tatcgctgaa gttgcgaagg aacaaggcgt taaaccttgc
     7801 cgtccgctct ctactgcagt ggtatccgcg cagattgcga tcaccgcatc gccaatctca
     7861 gcggcagtgg tttacatgtc ttccgtgatg gaaggtcatg gcatcagcta cctccatctg
     7921 ctctccgtgg tcatcccgtc caccctgctg gcggttctgg tgatgtcctt cctggtcact
     7981 atgctgttca actccaaact ctctgacgat ccgatttatc gcaagcgtct ggaagagggc
     8041 ttggttgaac tgcgcggtga aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc
     8101 tggctgttcc tgctgggcgt agttggcgtg gttatctatg caatcatcaa cagcccaagc
     8161 atgggtctgg ttgaaaaacc gctgatgaac accaccaacg caatcctgat catcatgctc
     8221 agcgttgcaa ctctgaccac cgttatctgt aaagtcgata ccgacaacat cctcaactcc
     8281 agcaccttca aagcaggtat gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc
     8341 gatactttcg tttccaacaa catcgactgg atcaaagata ccgctggtga agtgattcag
     8401 ggtcatccgt ggctgctggc cgtcatcttc ttctttgctt ctgctctgct gtactctcag
     8461 gctgcaaccg caaaagcact gatgccgatg gctctggcac tgaacgtttc accgctgacc
     8521 gctgttgctt ctttcgctgc ggtgtctggt ctgttcattc tgccgaccta cccgacgctg
     8581 gttgctgcgg tacagatgga tgacacgggt actacccgta tcggtaaatt cgtcttcaac
     8641 catccgttct tcatcccggg tactctgggt gttgccctgg ccgtttgctt cggcttcgtg
     8701 ctgggtagct tcatgctgta atgatcaatc gcggggcgtt cacgccccgc tttctttccc
     8761 gccgactaac atccttcccc cgtccgttgt atagtgacct ctctcttgcg gttccatctg
     8821 ttcttgcgag gtgtttatgc ttgatgaaaa aagttcgaat accacgtctg tcgtggtgct
     8881 atgtaccgca ccggatgaag cgacagccca ggatttagcc gccaaagtgc tggcggaaaa
     8941 actggcggcc tgcgcgacct tgatccccgg cgctacctct ctctattact gggaaggtaa
     9001 gctggagcaa gaatacgaag tgcagatgat tttaaaaact accgtatctc accagcaggc
     9061 actgctggaa tgcctgaagt ctcatcatcc atatcaaacc ccggaacttc tggttttacc
     9121 tgttacacac ggagacacag attacctctc atggctcaac gcatctttac gctgattctg
     9181 ctactttgca gcacttccgt ttttgccgga ttattcgacg cgccgggacg ttcacaattt
     9241 gtccccgcgg atcaagcctt tgcttttgat tttcagcaaa accaacatga ccttaatctg
     9301 acctggcaga tcaaagacgg ttactacctc taccgtaaac agatccgcat tacgccggaa
     9361 cacgcgaaaa ttgccgacga gcagctgccg caaggcgtct ggcatgaaga tgagttttac
     9421 ggcaaaagcg agatttaccg cgatcggctg acgcttcccg taaccatcaa ccaggcgagt
     9481 gcgggagcga cgttaactgt cacctaccag ggctgtgctg atgccggttt ctgttatccg
     9541 ccagaaacca aaaccgttcc gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct
     9601 gtgtctgttt cgcagcaaga gcagcacacc gcgcaattgc ccttttccgc gctctgggcg
     9661 ttgttgatcg gtattggtat cgcctttacg ccatgcgtgc tgccaatgta cccactgatt
     9721 tctggcatcg tgctgggtgg taaacagcgg ctctccactg ccagagcatt gttgctgacc
     9781 tttatttatg tgcaggggat ggcactgact tacacggcgc tgggtctggt agttgccgcc
     9841 gcagggttac agttccaggc ggcgctacag cacccatacg tgctcattgg cctcgccatc
     9901 gtctttacct tgctggcgat gtcaatgttt ggcttgttta ctctacaact cccctcttcg
     9961 ctgcaaacac gtctcacgtt gatgagcaat cgccaacagg gcggctcacc tagcggtgtg
    10021 tttgttatgg gggcgattgc cggactgatc tgttcaccat gcaccaccgc accgcttagc
    10081 gcgattctgc tgtatatcgc ccaaagcggg aacatgtggc cgggcggcgg cacgctttat
    10141 ctctatgcgt tgggcatggg cctgccgctg atgctaatta ccgtctttgg taaccgcttg
    10201 ctgccgaaaa gcggcccgtg gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc
    10261 gcactgccgg tcttcctgct ggagcgagtg attggtgatg tatggggatt acgcttgtgg
    10321 tcggcgctgg gtgtcgcatt ctttggctgg gcctttatca ccagcctaca ggctaaacgc
    10381 ggctggatgc gcgtggtgca aataatcctg ctggcagcgg cattggttag cgtgcgccca
    10441 cttcaggatt gggcatttgg tgcgacgcat accgcgcaaa ctcaaacgca tctcaacttt
    10501 acacaaatca aaacggtaga tgagttaaat caggcgctcg ttgaagccaa aggcaaaccg
    10561 gtgatgttag atctttatgc cgactggtgc gtcgcctgta aagagtttga gaaatacacc
    10621 ttcagcgacc cgcaggtgca aaaagcgtta gcagacacgg tcttacttca ggccaacgtc
    10681 acggccaacg acgcacaaga tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg
    10741 acaattctct tttttgacgg acaaggccag gagcatccac aagcacgcgt cacgggcttt
    10801 atggatgctg aaaccttcag cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca
    10861 gtgggaaaga cggaggaaaa tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa
    10921 ttattagaat tacaagggat tgccaacacc acgctggaga tggttgctga acgtgtggat
    10981 tatccactgg acgagctacg ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg
    11041 ctgcgttatc ttagccaaca gatagatgtc tggcgtcgtc agctgatgct ggacgaaacg
    11101 caaaccgccg aacaaaagct gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac
    11161 aaccgctatc cgggctgtct atttatcgct gcctgtacgt tttatcccga tcctggccac
    11221 cctattcatc aactggccga tcagcaaaaa agcgctgcct acgatttcac ccacgaactg
    11281 ttaaccacgc tggaagttga cgatccggcg atggtagcaa agcagatgga actggtgctg
    11341 gaaggctgtt taagccgaat gctggtgaat cgtagccagg cggatgtcga cactgcacat
    11401 cggctggcgg aag
//
LOCUS       DAJVPT010000116        11355 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVPT010000116 DAJVPT010000000
VERSION     DAJVPT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..396)
                     /locus_tag="OQM47_003264"
     CDS             complement(<1..396)
                     /locus_tag="OQM47_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1481304.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQL"
     gene            complement(407..796)
                     /gene="cheY"
                     /locus_tag="OQM47_003265"
     CDS             complement(407..796)
                     /gene="cheY"
                     /locus_tag="OQM47_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1481305.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(811..1860)
                     /gene="cheB"
                     /locus_tag="OQM47_003266"
     CDS             complement(811..1860)
                     /gene="cheB"
                     /locus_tag="OQM47_003266"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1481306.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(1863..2723)
                     /gene="cheR"
                     /locus_tag="OQM47_003267"
     CDS             complement(1863..2723)
                     /gene="cheR"
                     /locus_tag="OQM47_003267"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1481307.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2742..4343)
                     /gene="tap"
                     /locus_tag="OQM47_003268"
     CDS             complement(2742..4343)
                     /gene="tap"
                     /locus_tag="OQM47_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1481308.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4389..6050)
                     /gene="tar"
                     /locus_tag="OQM47_003269"
     CDS             complement(4389..6050)
                     /gene="tar"
                     /locus_tag="OQM47_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1481309.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6195..6698)
                     /gene="cheW"
                     /locus_tag="OQM47_003270"
     CDS             complement(6195..6698)
                     /gene="cheW"
                     /locus_tag="OQM47_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1481310.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6719..8683)
                     /gene="cheA"
                     /locus_tag="OQM47_003271"
     CDS             complement(6719..8683)
                     /gene="cheA"
                     /locus_tag="OQM47_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1481311.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLKTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8688..9614)
                     /gene="motB"
                     /locus_tag="OQM47_003272"
     CDS             complement(8688..9614)
                     /gene="motB"
                     /locus_tag="OQM47_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1481312.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9611..10498)
                     /gene="motA"
                     /locus_tag="OQM47_003273"
     CDS             complement(9611..10498)
                     /gene="motA"
                     /locus_tag="OQM47_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1481313.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10625..11203)
                     /gene="flhC"
                     /locus_tag="OQM47_003274"
     CDS             complement(10625..11203)
                     /gene="flhC"
                     /locus_tag="OQM47_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1481314.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11206..>11355)
                     /locus_tag="OQM47_003275"
     CDS             complement(11206..>11355)
                     /locus_tag="OQM47_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1481315.1"
                     /translation="RFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLNDVNQPEEAL
                     RKKRA"
BASE COUNT         2568 a         3188 c         2792 g         2807 t
ORIGIN      
        1 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
       61 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      121 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      181 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
      241 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgaaccagcc ccagttcccg
      301 caaactgtcg cgcagcatat gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
      361 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
      421 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
      481 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
      541 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
      601 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
      661 acttattgag agcgtcgaca ccatcttccg cttcctcaac attattgaat cccagctctt
      721 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
      781 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
      841 tgttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
      901 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
      961 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1021 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1081 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1141 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1201 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     1261 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     1321 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     1381 cgcaatcagt ttttcagaac tcaacaacgg ccctgccttc agcgttgtcg gtgctgacaa
     1441 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     1501 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     1561 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     1621 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     1681 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     1741 aaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     1801 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     1861 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcgagg
     1921 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     1981 aagcggcgca aaatctcctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag
     2041 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca
     2101 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2161 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     2221 tagataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     2281 cagcgtccgg gcacggtgcc caatgtgtca gccagagtca tcgcaatgct gtacggctct
     2341 tcgccggtcg aagccgccgc gctccatacg cgatcctcgc cagaacggcg acgtgcgtga
     2401 tccgcgagca gagggaaatg atgagcctca cggaaaaatg ccgtcagatt cgtggtcagc
     2461 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     2521 tgaccgaaat ccgtcagtcc cagagaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     2581 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     2641 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     2701 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     2761 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     2821 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     2881 tctaccagcg aggagttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     2941 tgctcaatcc cccgccgttg ttcttccgac gccgaagcaa tttctcccat aatgtcgttc
     3001 acgcgggtca ccgaactgac aatatcggtc atggtcgcgg cggcgttatt caccagtttc
     3061 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3121 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3181 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     3241 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     3301 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     3361 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     3421 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     3481 gagagatcgt tattgcctgc gacaatctcc gcgatcccaa tgtgcatctc atggcttccc
     3541 ttacgcacat cactcaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa
     3601 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     3661 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     3721 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc
     3781 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     3841 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     3901 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     3961 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4021 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4081 gtcatcaacg ttttaatgtc atccgccgga tagctaagcg ctgtcagagt gcctgctttg
     4141 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4201 tgattgctct gctcgccgtg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     4261 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     4321 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     4381 aaaactcgtc aaaatgtttc ccagtttgga tcttgttcag taattcgcag tcgtggctgt
     4441 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     4501 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     4561 gctgcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     4621 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     4681 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     4741 ccggcgcttt cgaccagcac cgaaccggta tcaatgcgcg agacggagtc ttcaatgagg
     4801 gctttgatct cttttgccgc cagggcgctg cgactggcaa gattacgcac ttcacccgcc
     4861 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     4921 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     4981 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5041 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5101 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5161 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     5221 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     5281 atatgtgaaa cgctctgcgc caggtcaccc atttcactgc gcccgtcaat ggtcagggta
     5341 ttcgccaggt taccaccggc gatttcgcaa atgtgagcaa taatttttgc cagcggagta
     5401 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     5461 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     5521 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     5581 attccctggg ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc
     5641 agttcagtta acgctgtgta atagttttta tatttttcat caaaattacg gctggtagcg
     5701 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc ggcctgggcc
     5761 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     5821 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     5881 caggttgacg tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc
     5941 tggctatggt gaagggaaga aaaaaacaaa ctgccggaaa taagctgtaa cagtgcgaat
     6001 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6061 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6121 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6181 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     6241 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     6301 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     6361 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     6421 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     6481 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     6541 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     6601 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     6661 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     6721 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     6781 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     6841 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     6901 gcaaggcgta gcggcgaccg ccactttgta agatcaccac aattccctgg gtggcttcgg
     6961 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7021 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7081 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca
     7141 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtaccct
     7201 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     7261 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc cgtggagaag cctggtgcaa
     7321 atatcagcat tgcaacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     7381 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     7441 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     7501 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     7561 tcagcgggtc gataatgcgt tctatcaggc tcttgtcgag ctcggtggaa ctgcccacca
     7621 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     7681 gactaaagac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     7741 gggcgttacg ttgtaactgc cccatgctgg taatcaaatc accatgatta accgggtcca
     7801 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     7861 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcactg ctgcgcgtcg
     7921 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcacaggtg
     7981 gggtagctat ttttggcgaa acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8041 caaaacagag taccgctgtg atgtcatctt cggcgatatc gcccggtaat attgccgaga
     8101 gagaatccgc ccctttcacc acgtcattta acgttgtcag atgccccagc tcttcttcca
     8161 gcaggtcgac ttcgccggcc ttcaggcgcg aaaggataat tcgtcgcggc aactgactgc
     8221 gactttgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     8281 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgtagc
     8341 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     8401 tcgtttccaa aaacagattg ataatgtcgg ttttgagttg catctcacct cgtctggctt
     8461 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     8521 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     8581 gcgcttccgg ctgcaaaacc agcagatgct gctccatatc agccaacagt tcgtccgctt
     8641 catcaaaaaa tgtctgataa aaatcgctta tatcgatgct cacgctgtca cctcggttcg
     8701 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc tagggcgctt
     8761 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     8821 agtaccaaca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     8881 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     8941 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct gtaacctttc
     9001 tcaccgctgg cgtagggaaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9061 ccgttcagca caggcgcgat ggcgcgcaga atgtcgcgca tatagggttc gacatcggca
     9121 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9181 tggaccagat cgattttgag atggagacgt aacgcccgca gtttcggatc ggactctatc
     9241 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcagc
     9301 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
     9361 ccgggaattg ggctttcact attggaaatg cgatcgccgc ccgtaaccgc tgtcgccagt
     9421 ggagtccgga agtactccgc aatctgaatc aactcttttg ggctggagat ggagatcagc
     9481 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
     9541 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
     9601 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
     9661 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
     9721 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
     9781 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct agtggggaaa
     9841 taaatccgta agccaataaa ataccgagga aagtccccac catcgcatgt gcgataagcg
     9901 cccccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
     9961 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10021 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10081 tgttcatgtg accgctgata atcaggcgca gataatcgac gataaaatca agcatgacgc
    10141 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10201 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    10261 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    10321 ctttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    10381 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    10441 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    10501 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    10561 tggggaaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg ccacaccgta
    10621 tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat
    10681 tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa
    10741 agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg
    10801 aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca
    10861 gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca
    10921 ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca
    10981 tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc
    11041 ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta
    11101 actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca
    11161 gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt
    11221 gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc
    11281 ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg
    11341 gtggctgtca aaacg
//
LOCUS       DAJVPT010000117        11231 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVPT010000117 DAJVPT010000000
VERSION     DAJVPT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            568..2820
                     /locus_tag="OQM47_003276"
     CDS             568..2820
                     /locus_tag="OQM47_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481316.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3156..4088
                     /gene="csiD"
                     /locus_tag="OQM47_003277"
     CDS             3156..4088
                     /gene="csiD"
                     /locus_tag="OQM47_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1481317.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4108..5376
                     /gene="lhgO"
                     /locus_tag="OQM47_003278"
     CDS             4108..5376
                     /gene="lhgO"
                     /locus_tag="OQM47_003278"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1481318.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5399..6847
                     /gene="gabD"
                     /locus_tag="OQM47_003279"
                     /pseudo
     CDS             5399..6847
                     /gene="gabD"
                     /locus_tag="OQM47_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6855..8141
                     /gene="gabT"
                     /locus_tag="OQM47_003280"
                     /pseudo
     CDS             6855..8141
                     /gene="gabT"
                     /locus_tag="OQM47_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8379..9779
                     /gene="gabP"
                     /locus_tag="OQM47_003281"
     CDS             8379..9779
                     /gene="gabP"
                     /locus_tag="OQM47_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1481319.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9800..10462
                     /gene="csiR"
                     /locus_tag="OQM47_003282"
     CDS             9800..10462
                     /gene="csiR"
                     /locus_tag="OQM47_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1481320.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10463..10912)
                     /gene="kbp"
                     /locus_tag="OQM47_003283"
     CDS             complement(10463..10912)
                     /gene="kbp"
                     /locus_tag="OQM47_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1481321.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10996..11154)
                     /locus_tag="OQM47_003284"
     CDS             complement(10996..11154)
                     /locus_tag="OQM47_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1481322.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2889 a         2751 c         2814 g         2777 t
ORIGIN      
        1 taaagggcct taagtatgca ataacaatac ttataaacca cgcaataaac atgatgatct
       61 tatgatgtaa ataacagatt tttatgcgtt cccattcacc ttgggtcgtg attacacgcg
      121 acataaaacc cgacaccgcc tccattcgca aagtcgatac tcgcagtcaa caagcaaatg
      181 ttaataatta gcactatcta tagttatcat cgattcaatg atactttgta atgattttgt
      241 atctaataat ataactttat tacattagct gaagagtttt cgcatcatta tgatatctgt
      301 tacttttcac tccataaaaa taaacttcat atagcaatat attctttcat agatcttatt
      361 ctgctaatca ttagtttcgt atgcgcgatt tttgacagtt gcctctccag accacatcga
      421 taattaaaaa acagatttaa acattatcct tttccatata gatattggat aaaaatagga
      481 catctgtttg caattacttt cacaacaatt aaacattttt atgttttcgt atacatcata
      541 ttactctacc attagaggaa cgttattatg ttttctataa aaccaggacc tggaaattta
      601 cctatcgaca accccacatt gttatcatgg aacattactg atggggatct aaattccaaa
      661 tttaatacat tagaatatct aaactgtata acaaatatta ttaatgcttg tggagtttat
      721 cctcaggatt taaaagacag agaaattata tcaacttttc acgcagaaaa agtcattaat
      781 gatctgttaa aaaacgatta taaaatttcc ctttctccag atacaactta ccgagagctg
      841 aataaagctg cacagagtag cattacagcg ccagacagga taggagaagg gaaaacatgg
      901 gtttatcaac gagatacaat ggttgaaaga ggtgataaca gtggtgttca tcagtatggt
      961 ccagctgaac atttcactca cattatatct gacaaaccgt ccccaaaaga taagtatgtt
     1021 gcatatgcta ttaacattcc tgactatgag ctagcagccg atgtatataa tattaacgtg
     1081 acgtcacctt ccggacagca agaaacattt aaaatactaa tcaatccaga acatctacgg
     1141 caaacacttg agcgtaaatc tcttactgct gttcagaaat cacaatgtga aatcatcacc
     1201 cccaaaaaac ctggcgaagc gattcttcat gcttttaatg ccacctacca gcaaatcagg
     1261 gaaaatatgt ctgagtttgc acgttcccat tatgggtata tacaaatccc tccagtgaca
     1321 actttccgcg ccgacggacc agaaactccc gaagaagaaa aaggttactg gtttcacgct
     1381 tatcaacccg aagatctttg taccatccat aatccaatgg gggatttgca ggattttatc
     1441 gcattggtta aagatgctaa aaaatttggt atcgatatca ttcctgatta tacctttaac
     1501 tttatgggaa tcgggggaag tggtaaaaat gacctggatt atccctctgc tgatatacga
     1561 gcgaagatca gtaaagatat agaaagtggt atccctggct attggcaagg tcaggttttg
     1621 attccattca ttaaagatcc agtaacaaaa gaacgtaaac aaatccatcc agaagatata
     1681 catctcactg caaaagactt cgaagcaagt aaagataaca tctctaagga tgaatggaaa
     1741 aacctccatg cattaaaaga aaagagttta aatggaatgc ctaaaacaac ccccaaaagt
     1801 gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga
     1861 ggtttacgtt atgatgcagc aaaacactca aaacatgaac aaatagaaag atcaataacc
     1921 ccaccgctta aaaattataa tgagcgatta cacaatacta acttatttaa cccaaaatat
     1981 catgaaaaaa ccgttatgaa ttacatggaa tatctggtaa cttgtcagtt gaatgaagaa
     2041 caaatgtcat cgctgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc
     2101 atgaagacga taaaagcctt ttcattcggg ggagatctcc taacccttgc atcaaaaccg
     2161 ggttccacaa tctcaagcat cccatcagaa agacggatat tgattaacat taaccacgat
     2221 tttcctaaca atggcaatct tttcaatgac tttctattta accatcaaca agatgaacaa
     2281 ttagcaatgg catatatggc cgctctcccg ttcagcaggc ctttagttta ctgggatggc
     2341 caggtattaa aatcaacgac tgaaattaaa aattatgatg ggcccacgcg tgtcggtggt
     2401 gaggcgtggc ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca
     2461 ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac
     2521 gttctggcct ttagtcgtgg ggattctgtg aacattaatc actctcctca tgatggacta
     2581 gttataataa ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa attgcaacct
     2641 ggaatatata aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa
     2701 attatccccc cttgtaaagt atttacgctt agaggcggat ctctaaatat caatattcct
     2761 aggcgtagcg ctcttctttt agggaaaaca ggagaaccgc cgaactatct ctatttataa
     2821 tttaatgtta tatctgcccc gataaaacgg ggcagataat atgtttagtt tactaacggt
     2881 cattttgtag tgaagccatt tactgttttt tatcgaccag ataatctgtt ctctaatgtt
     2941 aactccccct aacctgttgc tttagttatt catttcctgt ctcactttgc cttaataccc
     3001 tacgttaaat gttactaatt tgttgctttt gatcacaata agaaagcaat atgtcgcttt
     3061 tgtgcgcatt tttcagaaat gtagatattt ttagattatg gctacgaaat gagcatcgcc
     3121 atgtcaccct acatctcata agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa
     3181 ataacgctgt cgattcaggc caggactata gcggattcac tctcatcccg tcggcgcaat
     3241 ccccgcgtct gctggaactc accttcaccg aacagacgac caaacagttt ctcgaacagg
     3301 ttgccgaatg gcccgtgcag gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca
     3361 aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt gctgttgaag accctgctaa
     3421 accgtgctga aggtgcgctg ttgatcaatg cggtaggtat cgatgatgtg gcgcaggcag
     3481 atgagatggt gaagctggca acggcggtag cgcatctgat tggtcgctca aatttcgatg
     3541 cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa tgtcgataac tcagacagct
     3601 atctgcgtca gccgcaccgc gtgatggaac tgcacaacga cggcacgtat gtcgaagaga
     3661 tcaccgatta cgtgctgatg atgaaaatcg atgactggga acatctggac cactatttcc
     3721 gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa aacgtcagca
     3781 aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg atgcgctata
     3841 tcgaccagtt cgtccagcca aaagattttg aagaaggcgt gtggttgagc gagctttccg
     3901 atgccattga aaccagcaaa ggcattcttt ctgtacccgt tcccgttggc aaattcctgt
     3961 tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac ccggatctgc
     4021 gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac taccagacgc
     4081 atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg cggcggcatc
     4141 atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg cattgcgttg
     4201 ctggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagcgg cgtgatccat
     4261 gccggggtct attacacgcc cggcagcctg aaggcgcagt tttgcctggc ggggaaccgt
     4321 gccaccaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg caagatgctg
     4381 gtcgccacgt ctcagctcga aatggaacgg atgcgcgcct tatgggaacg cacagcggcg
     4441 aacggtatcg agcgcgagtg gataaacgcc gtggaactgc gcgagcgcga accgaatatc
     4501 accgggcttg gtggcatttt tgtgccgtcc agcggcattg tcagctaccg cgaagtgacg
     4561 gcggcaatgg caaaaatctt ccaggccaga ggcggcgaga ttatttataa cgccgaagtc
     4621 agcgccctca gtgagcataa aaacggcgtg gtgatacgca cccgtcaggg cggtgaatat
     4681 gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt gaaaatgctc
     4741 ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg ccttgcgccg
     4801 gagcataacc agattgttaa ccacctgatt taccccattc ccgaccccgc gatgccattt
     4861 ttgggcgtgc atctcacccg catgatcgac ggcagcgtga ccgtcgggcc aaacgcggtg
     4921 ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagtga cacgctggag
     4981 attttgagct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc aggactgggc
     5041 gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa gtattgtccc
     5101 cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca ggcagtatcg
     5161 ccggacggca agctgattga cgattttctg tttgtcacca ccccacgcac gatccacacc
     5221 tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca tattgtcagc
     5281 aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg agcggcacgt
     5341 agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg acgtaacaat
     5401 gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg aatggctgga
     5461 cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca agctgggtag
     5521 cgtgccgaaa atgggcgcag atgaaacccg cgccgctatc gacgccgcca accgcgccct
     5581 gcccgcctgg cgtgcgctca ccgccaaaga acgcgccacc attctgcgca actggttcaa
     5641 tttgatgatg cagcatcagg acgatttagc gcgcctgatg accctcgaac agggtaaacc
     5701 gctggctgaa gcgaaaggcg aaatcagcta cgccgcctcc ttcattgagt ggtttgctga
     5761 agaaggcaaa cgtatttatg gcgacaccat tcctggtcat caggccgata agcgcctgat
     5821 tgttatcaag cagccgattg gcgtcaccgc cgccatcacg ccgtggaact ttccggcggc
     5881 gatgattacc cgtaaagccg gtccggcgct ggcggcaggc tgcaccatgg tgctgaagcc
     5941 cgccagtcag acgccgttct ctgcgctggc gctggcggaa ctggcgattc gcgcgggcat
     6001 tccggctggc gtgtttaacg tggtcaccgg ttcggcaggc gcggtcggta atgaactgac
     6061 cagcaacccg ctggtacgca aactgtcgtt taccggttag accgagattg gccgccagtt
     6121 aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg gcaacgcgcc
     6181 gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc tggcctcgaa
     6241 attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc aggacggcgt
     6301 gtatgaccgc tttgccgaaa aattacagca ggcggggagc aaactgcaca tcggcgacgg
     6361 gctggagaaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag caaaagtgga
     6421 agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg gtaaagcgca
     6481 cgaacgcggc ggcaacttct tccagccaac cattctggtg gacgttccgg ccaacgccaa
     6541 agtgtcgaaa gaagagacgt tcggccccct cgccccactg ttccgtttta aagatgaagc
     6601 cgatgtgatt gcacaagcca atgacaccga gtttggtctt gccgcctatt tctacgcccg
     6661 tgatttaagc cgcgtcttcc gcgtcggcga ggcgctggag tacggcatcg tcggcatcaa
     6721 taccggcatt atttccaatg aagtggcccc attcggcggc atcaaagcct cgggtctggg
     6781 tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg
     6841 tctttaactg gagaatgcga aggaacagca ataaagagtt aatgcagcgc cgcagtcagg
     6901 cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg gaaaactgcc
     6961 gggtgtggga cgtttaaggc cgtgagtatc ttgatttcgc gggcgggatt gcggtgctca
     7021 ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg aaaaaactgt
     7081 cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc gagattatga
     7141 atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc ggttccgaag
     7201 cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc accatcgctt
     7261 ttagcggcga gtatcacggg cgcacgcatt acacgctggc gctgaccggc aaggtgaatc
     7321 cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt tatccttgcc
     7381 cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc ttcaaaaatg
     7441 atgccgcgcc ggaagatatc gccgccatca tgattgagcc ggttcagggc gaaggcggtt
     7501 tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc tctgtgtgac gagcacggga
     7561 tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg ctgtttgcga
     7621 tggagcagat gggcgtggcg ccggatctta ccacctttgc gaaatcgatc gcaggcggtt
     7681 tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga cgctgttgca ccgggcggtc
     7741 tgggcggcac ctatgccggt aacccgattg cctgcgtggc tgcgctggaa gtgttgaagg
     7801 tgttcgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag ctaaaagacg
     7861 gattgttggc gatagccgaa aaacacccgg agatcggcga cgtacgcggg ctgggatcga
     7921 tgattgccat cgagctgttt gaagatggcg atcacagcaa accggacgca aaactcaccg
     7981 ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct tctctcctgc ggcccgtatt
     8041 acaacgtgct gcgcatcctt gtaccactca ccattgaaga cgctcagatc cgtcagggtc
     8101 tggagattat cggtcagtgt tttgatgagg cgaagcagta gcaccgtccc catgcaggag
     8161 gcgacgctgc gcgtcttgtc cggcctacga gaatccaagt cggataaggc gtttacgccg
     8221 catccgacaa tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg
     8281 ctctccggca aaaagaatca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg
     8341 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
     8401 acgagttagg cggcgggctg aaatcacgcc acgtcaccat gttgtctatt gccggtgtta
     8461 tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcggac ccggcggtat
     8521 tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg ctggcggaaa
     8581 tggcagttgc cacgcccgat accggttcgt tttccaccta tgccgataaa gccattggcc
     8641 gctgggcggg ctataccatc ggctggttgt actggtggtt ttgggtactg gttatcccac
     8701 tggaagccaa catcgccgcc atgatcctgc actcgtgggt tccaggcatt cccatctggt
     8761 tattttccct cgtcattacc ctcgccttaa ctggcagtaa tttattaagc gttaaaaact
     8821 acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg gcctttattt
     8881 tccttggtgc agtcgcaatt agcggttttt acccttatgc cgaagtgagc gggatctcaa
     8941 gattgtggga tagcggcggc tttatgccca acggtttcgg tgcggtatta agcgcgatgt
     9001 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg
     9061 acatgccgga aaaacatatt gtccgcgcca ccaactcggt tatctggcgt atttctatct
     9121 tctatttgtg ctctattttt gtcgtcgtgg cattaatacc gtggaatatg ccggggctga
     9181 aagccgttgg atcttatcgc tcggttctgg aattgctcaa tattccccat gcgaaattaa
     9241 tcatggactg tgtgatatta ctttccgtaa ccagttgcct gaactcggcc ctgtataccg
     9301 cctcaaggat gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa
     9361 tcaaccgcag taaaaccccg tacgtggcgg tgttactctc caccggcgca gcattcttaa
     9421 cggtggtggt gaactattac gcgcctgcga aggtatttaa atttctgatc gacagctccg
     9481 gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa
     9541 ttctgcgagc agaaggaagc gaaattcgct tgcgaatgtg gcttgatccc tggctcacct
     9601 ggctggtcat cggctttatt acctttgtgt tggtagtgat gctattccgt ccggcgcaac
     9661 agttagaagt gatctccacc ggcttattag cgatagggat tatctgtacc gtgccaatta
     9721 tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctgat
     9781 aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa
     9841 cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cggatgagtt tactgacatc
     9901 gcgttatgca cttggcgttg ggccgttgca ggaagcactt tctcaactgg tggcggaacg
     9961 gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct
    10021 gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ctggtcagtc tggcgattgc
    10081 ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct
    10141 ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
    10201 tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
    10261 gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc tttcggtgga
    10321 aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac tgccgcgaga
    10381 tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
    10441 gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc cccggataaa
    10501 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag
    10561 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
    10621 actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg gcaataccgg
    10681 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag
    10741 tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
    10801 ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
    10861 ccgcatccca gagtttttct ccggcatctt taacaaaatt gaacagaccc atagctacct
    10921 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
    10981 ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    11041 atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    11101 cagcacgccg atcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    11161 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
    11221 ttagcggagc c
//
LOCUS       DAJVPT010000118        11192 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVPT010000118 DAJVPT010000000
VERSION     DAJVPT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            328..525
                     /locus_tag="OQM47_003285"
     CDS             328..525
                     /locus_tag="OQM47_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481323.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(475..1668)
                     /locus_tag="OQM47_003286"
     CDS             complement(475..1668)
                     /locus_tag="OQM47_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1481324.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1804..3528
                     /gene="iucA"
                     /locus_tag="OQM47_003287"
     CDS             1804..3528
                     /gene="iucA"
                     /locus_tag="OQM47_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1481325.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3529..4476
                     /gene="iucB"
                     /locus_tag="OQM47_003288"
     CDS             3529..4476
                     /gene="iucB"
                     /locus_tag="OQM47_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1481326.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4476..6218
                     /gene="iucC"
                     /locus_tag="OQM47_003289"
     CDS             4476..6218
                     /gene="iucC"
                     /locus_tag="OQM47_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1481327.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6215..7552
                     /gene="iucD"
                     /locus_tag="OQM47_003290"
     CDS             6215..7552
                     /gene="iucD"
                     /locus_tag="OQM47_003290"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1481328.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7558..9753
                     /gene="iutA"
                     /locus_tag="OQM47_003291"
     CDS             7558..9753
                     /gene="iutA"
                     /locus_tag="OQM47_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1481329.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10304..10447)
                     /locus_tag="OQM47_003292"
     CDS             complement(10304..10447)
                     /locus_tag="OQM47_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481330.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10686..>11192
                     /locus_tag="OQM47_003293"
     CDS             10686..>11192
                     /locus_tag="OQM47_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1481331.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAE"
BASE COUNT         2796 a         2810 c         2906 g         2680 t
ORIGIN      
        1 tatcagtggt tcagggatga attaaaaacc agtaaaaata cccgacaaga aatctgcaat
       61 gatttacttc ataaaaaaca tgtcagtgaa aaagaaacct ggcgttgggt tgaaggatat
      121 aataaagcaa cagaactggc tcgccaatgc cctgataccc atgtattgtc aatttctgat
      181 cgtgaaggcg atttttatga cctgtttgaa cgcgcagcgc agactcccgg aataaaagct
      241 gactggcttg tcagaatgaa atttaataac agagcaacgc tgaatattaa tggtaaacgg
      301 gatcataggt tgttgcacga aagaatcatg gagattacac cgcagcaact ggttgaattc
      361 actatacctg atgggcgggg gcagcaagcc agaacactgc ctgacatgct ttcatcagac
      421 gcaggtcctc tctcatatcc tctgatttac tccagtaagg tgatgatcat atctttattt
      481 gatagtacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      541 tgctgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      601 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      661 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      721 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      781 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      841 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      901 ttggctaatg gcggtggtat ccgtagcgaa ctggcgagta agggcaggcg aaagtccaag
      961 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
     1021 ccgcttcaga ctgacgctct tgcactccgg tgtgggctgt ggtggtgtgc cgggcaaccg
     1081 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
     1141 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
     1201 ggagctgatg gttgccagag cggccatgcg attcccttct ccagctctct gcaatgccca
     1261 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1321 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1381 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1441 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1501 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1561 gagcacaacc agcagaccgt tctgtccgat accgagaagc ccggcactga acgcaagtgg
     1621 ccagcaggac agtggttttt ggggcgtatc ttcgatccct ggtgacatta taatgtttca
     1681 actccatgta ttaattgtgt ttatttgtaa aattaattta tctgacaata acatttattg
     1741 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1801 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1861 gcactgattc gtgaaaccac agactggaaa ctgacagaat acccgccaga cgaattgctc
     1921 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     1981 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     2041 tttaccactc tctcccggct tattattgat aagctacgcc atcaactgtt tctgcccgtt
     2101 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc atgtccatac gcaacaggca
     2161 attgatgccc gtcatgactg ggccgctctg cgtgaaaaag cgttgaattt tggcgaggct
     2221 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     2281 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2341 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2401 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2461 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2521 gcacttgttg ctaaagggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2581 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2641 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2701 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2761 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2821 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagact
     2881 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     2941 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     3001 gttgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     3061 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     3121 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     3181 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     3241 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3301 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3361 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3421 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccattgt tgacccttcg
     3481 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3541 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3601 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3661 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3721 ccctgggccg aatggcgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3781 gacattatcc atcgtactgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3841 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3901 ccccgtccac agggtgaggt ttaccgccgt tacgatccac gtattcgcag gatgctgagt
     3961 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     4021 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     4081 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     4141 agctatttcg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     4201 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     4261 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4321 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4381 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4441 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4501 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4561 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttca
     4621 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4681 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4741 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4801 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4861 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4921 gcaaagaggc gctggaacga tatgcgccag agtataccaa caccttcaga ctgcactggc
     4981 tggcggtaaa acgtgaacat atgatctggc gctgcgataa cgatctggat attcagcagt
     5041 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     5101 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     5161 tcgccacaga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     5221 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     5281 ggctggatat caagctgccg ctgaccatct acaatacctc atgctaccgg gggatccctg
     5341 gcagatatat cgctgccgga ccactggctt cacgctggct gcaacaggtt tttgcgaccg
     5401 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5461 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5521 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtcct gttctgatgg
     5581 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5641 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5701 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5761 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5821 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5881 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     5941 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     6001 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatggca gagcgttttg
     6061 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     6121 cctggccgga cctggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     6181 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     6241 tgtcgggaca gggccattta atctcagcat tgctgcattg tcacatcaga tcgaagaact
     6301 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6361 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6421 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6481 caggctacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6541 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6601 attgtttctg gttcagacca gtcggggaga atattttgcc cacaatatct gccttggtac
     6661 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6721 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataaccg tggttggtgg
     6781 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6841 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6901 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     6961 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     7021 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     7081 tctgctaccc agtcgctcgg taacaactct ggaaagtagt ggtccgggct ggaggttatt
     7141 gatggaacat catctggatc agggcagaga gagcctggaa agtgatgtgg tgattttcgc
     7201 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     7261 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7321 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7381 gctcagcctg atggcctggc gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7441 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7501 acagccggag gctgcttctt taactcacta tacagcaaat attcaggaat aacgatgatg
     7561 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7621 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7681 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7741 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7801 agccagagtc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7861 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7921 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     7981 accggagggt tgatcaacat cgtgaccaaa aaaggtcagc cggaaaccat gatggagttt
     8041 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     8101 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     8161 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     8221 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     8281 cagcttcaac tgataacgca gtactataaa agtcagggag acgacaatta cgggcttaat
     8341 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8401 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8461 gatttcctgg gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggtac
     8521 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8581 gataccgacc agtacggcat gaaactgact ctgaacagcc aactgatgga cggctggcaa
     8641 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8701 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8761 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8821 gatattttta ccgttagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8881 ttcatcgact acacgcagca acagaagatt gctaccggga aggcgatatc tgccgacgcc
     8941 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     9001 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     9061 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     9121 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     9181 ggctggcgct ttatcggtga caacctgcgg actcaaatcg cggcatatta ctcgctttcc
     9241 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9301 atttacggcg tggaaggtgc ggtggactac ctgatcccgg atactgactg gagtaccggt
     9361 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9421 gtgaaggaat caagtccatc gaaagcgaca gcttacatta actgggcgcc ggaaccgtgg
     9481 agtctgcgtg tacagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9541 aatggttaca ctaccgtcga ttttatcagt agttggcagc ttccggtggg aacactcagc
     9601 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9661 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9721 acctttggtc tgaactactc agtgctgttc tgacggggat gcttttgcaa caagggtatg
     9781 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtataat atacagcctt
     9841 aacaagatat tccgtctccg gaatgggctg ttcaccactg aataacatta tgtctgtttt
     9901 atggatcttt gactgaaatt tatcgcatat cctcatttca ttgtccgtct gaaaactaac
     9961 cttacaagta agcgtcgtct cagcaccgtc tggcagatcc tgaaattcct gagaggatga
    10021 tctgcgccca atatcctgag ccggaacttt gctgcaatga aggccaacga aaaatggggg
    10081 agtatgttat agagtacttg ctacagggta aaaagaggta cctgtcgctg gttctccgtt
    10141 cagtttaacc gggagatttt ctcctgcgcc tgtcataaag tccggtaatg gagattgtta
    10201 atacgatggt gcacgatgca tcgtcagtgc ccggcccgga ggatgccccg tagctacata
    10261 cggatcaggg ttggcaatat cgaatggcag gctataggca aagttaaagc cccatggagt
    10321 agcacaaaat atgccgcaca aaggaaacgg tctgaataac gcagtgatgg agaacttctt
    10381 cggcacactg caaaatcgca tcgtggtcga tcgctaattc gtcgtgaatc actgaacgat
    10441 gttccatgcg aacagatatt cgatcatatc gaaatattaa acattacccc tccccacatt
    10501 gggcaaaaaa aaacaaaaaa cgatcaatta gtattgattc acataatctc tcatcattaa
    10561 tgagaacatt gtcacatatt tacaatatag aactgttact tttagcaagc tgttagtaac
    10621 caaccgttac taataatttt aattcttaat actttgttca ttcaataaat gagttgagag
    10681 aatatatgaa taaaatatac tcccttaaat atagtgctgc cactggcgga ctcattgctg
    10741 tttctgaatt agcgaaaaga gtttctggta aaacaaaccg aaaacttgta gcaacaatgt
    10801 tgtctctggc tgttgcaggt acagtaaatg cagcaaatat tgatatatca aatgtatggg
    10861 cgagagacta tcttgatctt gcacaaaata aaggtatttt ccagcccgga gcaacagacg
    10921 taacaatcac tttaaaaaac ggagataaat tctctttcca taatctctca attccggatt
    10981 tttctggtgc agcagcgagt ggcgcagcta ccgcaatagg aggttcttat agtgttactg
    11041 ttgcacataa caaaaagaac cctcaggccg cagaaactca ggtttacgct cagtcttctt
    11101 acaaggttgt tgacagaaga aattccaatg attttgagat tcagaggtta aataaatttg
    11161 ttgtggaaac agtaggtgcc accccggcag ag
//
LOCUS       DAJVPT010000119        11178 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVPT010000119 DAJVPT010000000
VERSION     DAJVPT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            397..480
                     /locus_tag="OQM47_003294"
     CDS             397..480
                     /locus_tag="OQM47_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1481332.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(534..1886)
                     /gene="gorA"
                     /locus_tag="OQM47_003295"
     CDS             complement(534..1886)
                     /gene="gorA"
                     /locus_tag="OQM47_003295"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1481333.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(1958..2800)
                     /gene="rlmJ"
                     /locus_tag="OQM47_003296"
     CDS             complement(1958..2800)
                     /gene="rlmJ"
                     /locus_tag="OQM47_003296"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1481334.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3003..5045
                     /gene="prlC"
                     /locus_tag="OQM47_003297"
     CDS             3003..5045
                     /gene="prlC"
                     /locus_tag="OQM47_003297"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1481335.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5053..5805
                     /gene="rsmJ"
                     /locus_tag="OQM47_003298"
     CDS             5053..5805
                     /gene="rsmJ"
                     /locus_tag="OQM47_003298"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1481336.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5854..7323)
                     /gene="dtpB"
                     /locus_tag="OQM47_003299"
                     /pseudo
     CDS             complement(5854..7323)
                     /gene="dtpB"
                     /locus_tag="OQM47_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7641..8075)
                     /gene="uspA"
                     /locus_tag="OQM47_003300"
     CDS             complement(7641..8075)
                     /gene="uspA"
                     /locus_tag="OQM47_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1481337.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8469..8804
                     /gene="uspB"
                     /locus_tag="OQM47_003301"
     CDS             8469..8804
                     /gene="uspB"
                     /locus_tag="OQM47_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1481338.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8875..10374)
                     /gene="pitA"
                     /locus_tag="OQM47_003302"
     CDS             complement(8875..10374)
                     /gene="pitA"
                     /locus_tag="OQM47_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1481339.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10606..>11178
                     /locus_tag="OQM47_003303"
     CDS             10606..>11178
                     /locus_tag="OQM47_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HCS1481340.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGL"
BASE COUNT         2734 a         2877 c         3003 g         2564 t
ORIGIN      
        1 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
       61 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
      121 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
      181 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
      241 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
      301 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
      361 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
      421 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
      481 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
      541 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
      601 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
      661 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
      721 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
      781 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
      841 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
      901 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
      961 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     1021 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     1081 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     1141 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     1201 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     1261 atcgggtcga agctgcgcag cggcgcatgc ttacgcacaa acagatgcgt tttcgcgccg
     1321 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     1381 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     1441 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     1501 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     1561 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     1621 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     1681 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     1741 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     1801 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     1861 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     1921 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     1981 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     2041 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     2101 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     2161 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     2221 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     2281 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     2341 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     2401 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     2461 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     2521 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     2581 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     2641 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     2701 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     2761 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     2821 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     2881 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     2941 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     3001 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     3061 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     3121 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     3181 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     3241 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     3301 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     3361 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     3421 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     3481 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     3541 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     3601 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     3661 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     3721 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     3781 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     3841 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     3901 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     3961 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     4021 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     4081 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     4141 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     4201 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     4261 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     4321 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     4381 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     4441 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     4501 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     4561 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     4621 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     4681 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     4741 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     4801 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     4861 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     4921 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     4981 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     5041 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     5101 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     5161 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     5221 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     5281 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     5341 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     5401 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     5461 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     5521 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     5581 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     5641 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     5701 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     5761 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     5821 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     5881 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     5941 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     6001 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     6061 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6121 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6181 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6241 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6301 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6361 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6421 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6481 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6541 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6601 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     6661 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     6721 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     6781 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     6841 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     6901 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     6961 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7021 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7081 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7141 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7201 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7261 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7321 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7381 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7441 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7501 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7561 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7621 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     7681 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     7741 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     7801 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     7861 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     7921 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     7981 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8041 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8101 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8161 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8221 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8281 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8341 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8401 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8461 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8521 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8581 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     8641 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     8701 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     8761 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     8821 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     8881 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     8941 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9001 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9061 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9121 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9181 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9241 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9301 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9361 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9421 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9481 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9541 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9601 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
     9661 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
     9721 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
     9781 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
     9841 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
     9901 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
     9961 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10021 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10081 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10141 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10201 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10261 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10321 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10381 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10441 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10501 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10561 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10621 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    10681 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    10741 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    10801 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    10861 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    10921 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    10981 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11041 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11101 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11161 ccgacccgcg cgggtctg
//
LOCUS       DAJVPT010000120        10821 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVPT010000120 DAJVPT010000000
VERSION     DAJVPT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..449)
                     /gene="intS"
                     /locus_tag="OQM47_003304"
     CDS             complement(<1..449)
                     /gene="intS"
                     /locus_tag="OQM47_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1481341.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFE"
     gene            complement(611..685)
                     /locus_tag="OQM47_003305"
     tRNA            complement(611..685)
                     /locus_tag="OQM47_003305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Arg,seq:cct)
     gene            complement(759..1691)
                     /locus_tag="OQM47_003306"
                     /pseudo
     CDS             complement(759..1691)
                     /locus_tag="OQM47_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1985..2740
                     /gene="mlaA"
                     /locus_tag="OQM47_003307"
     CDS             1985..2740
                     /gene="mlaA"
                     /locus_tag="OQM47_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1481342.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2922..3980)
                     /locus_tag="OQM47_003308"
     CDS             complement(2922..3980)
                     /locus_tag="OQM47_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481343.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4346..5686)
                     /gene="fadL"
                     /locus_tag="OQM47_003309"
     CDS             complement(4346..5686)
                     /gene="fadL"
                     /locus_tag="OQM47_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1481344.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6058..6342
                     /locus_tag="OQM47_003310"
     CDS             6058..6342
                     /locus_tag="OQM47_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1481345.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6522..7832
                     /gene="fadI"
                     /locus_tag="OQM47_003311"
     CDS             6522..7832
                     /gene="fadI"
                     /locus_tag="OQM47_003311"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1481346.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7832..9976
                     /gene="fadJ"
                     /locus_tag="OQM47_003312"
     CDS             7832..9976
                     /gene="fadJ"
                     /locus_tag="OQM47_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1481347.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10179..10664
                     /gene="sixA"
                     /locus_tag="OQM47_003313"
     CDS             10179..10664
                     /gene="sixA"
                     /locus_tag="OQM47_003313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1481348.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2683 a         2510 c         2702 g         2926 t
ORIGIN      
        1 tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg aatctcaagg
       61 ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt cgcataccct
      121 actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact aaatgaattg
      181 ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat gtctttcctc
      241 gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt tccgacggtc
      301 aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt cccggataca
      361 gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc tttcggcttt
      421 gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa aattatttta
      481 tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac catgacgaac
      541 tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg gatgggagcg
      601 aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta ggaggggctc
      661 gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg gactgcgggg
      721 taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt tttatcattt
      781 ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg cttgttgctc
      841 atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc gccgcagatg
      901 ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg cagcgtaccg
      961 ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt gtcacccagg
     1021 gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc tgcaggaaac
     1081 atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa catctcgctg
     1141 ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt ttcttcattg
     1201 aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat attacccagc
     1261 agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg tttttgcatg
     1321 acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc cattatgacg
     1381 ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg cacaccttcc
     1441 agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag acccgccgca
     1501 atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc atcctggcgg
     1561 atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat ttctattttt
     1621 ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg atcaattttg
     1681 tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta gcggcttttt
     1741 tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt gcctttttct
     1801 gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat caaatcagca
     1861 aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc taaacaggcg
     1921 gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa acagggagac
     1981 atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg tggggtgtgc
     2041 gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca accgcaccat
     2101 gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg ctgtcgcctg
     2161 gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg gcaaccttga
     2221 agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg ggatggtcca
     2281 ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg atgttgcagg
     2341 gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta cgcttggtca
     2401 ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct tcacgctgcg
     2461 tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc tgacttggcc
     2521 gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc tgctggattc
     2581 cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag cgtacttcca
     2641 gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc cgaacgcgca
     2701 agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata aaaaaggtga
     2761 gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa aggagataaa
     2821 ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg actatttccg
     2881 atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc atcaaaatta
     2941 acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg attttgaagt
     3001 cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc aggatggtca
     3061 acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc gcgtctaaat
     3121 tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt aaaaacttcg
     3181 ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc ggaaccagtt
     3241 gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc actcctggcg
     3301 ttttcactac aatattgggt gagactatca ccattaatac gaattcctct ttgaatgtgg
     3361 tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt atttttaatt
     3421 acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa gtccgttggt
     3481 tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg tgatatctta
     3541 tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa gatcatttcc
     3601 ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt acggacgagt
     3661 tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat accaatagta
     3721 atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt attaatatta
     3781 gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag tagatgttta
     3841 atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg tactaatcca
     3901 ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct ggtattgttg
     3961 attgaaatag atggtagcat gattttattc catgttgatt gatttgttac aaatatatat
     4021 tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc tttaatttga
     4081 gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga aaagtaaact
     4141 ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt ttgtctgtgg
     4201 ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa actcaggagg
     4261 caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct gcatagcagg
     4321 tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta ccgaacagcc
     4381 aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg ctctgaccgt
     4441 gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa gtcgtacctg
     4501 cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga gccggaactg
     4561 ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag taataagtgg
     4621 tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac agcgtgtcgc
     4681 cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa gccaggctat
     4741 agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac atttcaggca
     4801 ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga attggcaaac
     4861 cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag tcaattttca
     4921 cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac aggattccgg
     4981 cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta ttactgtcga
     5041 taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca ggagattgca
     5101 taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt ttcgcgcgag
     5161 cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga tacgcaccgc
     5221 ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg cctgcataag
     5281 tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc caaccaaatt
     5341 ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc gcgatgttat
     5401 cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc gggtcaatat
     5461 aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg gggttacggc
     5521 taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg cccaggccag
     5581 aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag ataagtgcca
     5641 ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc ataacctcaa
     5701 tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag tgtagggtct
     5761 gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag ctagaatgtt
     5821 gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca gctggcaaaa
     5881 tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt tttaagtgtg
     5941 atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt tccggaagcg
     6001 taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa atcaacgatg
     6061 agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg caccattatg
     6121 gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc agaagcagaa
     6181 caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc gtgcaaaatc
     6241 tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt cacttttgcc
     6301 tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc gggtacgtac
     6361 gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc actgtgtcat
     6421 agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta agactatgat
     6481 caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag gttttaccac
     6541 tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg ccttttgccc
     6601 gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg gtggtaggcg
     6661 aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc tttggtcagg
     6721 tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt accggaatga
     6781 atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc caagcggttg
     6841 caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc ggtggggcag
     6901 attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg ctggttgatg
     6961 tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg cgtttgcgcg
     7021 acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg atgggcgaca
     7081 ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat gcattagcgc
     7141 accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa gaagaggtga
     7201 tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat attcgcggta
     7261 attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa cacggaacgg
     7321 taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc ctgatgactg
     7381 aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc tacgcattta
     7441 ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca ccgctggcgc
     7501 tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac gaagcctttg
     7561 cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct cgtgaagtac
     7621 tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg cttggcggtt
     7681 cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc cagacattgc
     7741 atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct gccggtgggc
     7801 ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt ttactcttaa
     7861 tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga aaatgaatac
     7921 cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc gtgaaaacaa
     7981 agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg ccggagcaga
     8041 catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg cgcggcaggg
     8101 ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg ctattcatgg
     8161 cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg tttgtactga
     8221 cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac ccggttcagg
     8281 cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga tgattctcac
     8341 cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg acgttgttcc
     8401 gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc catcttcccg
     8461 ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc tgctgttcaa
     8521 aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga cagaacgcat
     8581 cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg acgctgaagc
     8641 tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta gtatcttttt
     8701 tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc cattaaacag
     8761 cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca ctgcttgtaa
     8821 agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc atgcgctgaa
     8881 gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag ccagcgaacg
     8941 tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg cccatcgcga
     9001 tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg tggcggaagt
     9061 ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt taccgattgg
     9121 tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt tcttcagtcc
     9181 ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg cgcaaaccat
     9241 cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg tgcgtgacaa
     9301 agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta tccgcatgct
     9361 gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg gttttccggt
     9421 aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa ttattcctgt
     9481 actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt cttcaatttt
     9541 gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg gtcagaaagg
     9601 gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg cacaagggca
     9661 ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc tgaatgaagc
     9721 agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata ttggtgcggt
     9781 atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg attctctcgg
     9841 cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt cccgttttac
     9901 cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga aaacaactgc
     9961 aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg cggagtgttg
    10021 gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa cagcgtttca
    10081 ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt ttcagatgaa
    10141 agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt atcatgcgtc
    10201 acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc actaatggtt
    10261 gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa atcgaacgcg
    10321 ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg gattgtctga
    10381 acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat gttggtctgg
    10441 tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg gtgatctctc
    10501 acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg ccgcctatgt
    10561 ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt acattcaact
    10621 ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt ccaaattatg
    10681 cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat ctgtcaacct
    10741 cactactatc gaagactctc cttatctata aataaaacac tttctacatt cattcggaaa
    10801 ttattcttct acgcgtatct t
//
LOCUS       DAJVPT010000121        10792 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVPT010000121 DAJVPT010000000
VERSION     DAJVPT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            154..984
                     /locus_tag="OQM47_003314"
     CDS             154..984
                     /locus_tag="OQM47_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1481349.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1125..1898)
                     /gene="uxuR"
                     /locus_tag="OQM47_003315"
     CDS             complement(1125..1898)
                     /gene="uxuR"
                     /locus_tag="OQM47_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1481350.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2113..3573)
                     /locus_tag="OQM47_003316"
     CDS             complement(2113..3573)
                     /locus_tag="OQM47_003316"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1481351.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3654..4838)
                     /gene="uxuA"
                     /locus_tag="OQM47_003317"
     CDS             complement(3654..4838)
                     /gene="uxuA"
                     /locus_tag="OQM47_003317"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1481352.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5178..6521
                     /gene="gntP"
                     /locus_tag="OQM47_003318"
     CDS             5178..6521
                     /gene="gntP"
                     /locus_tag="OQM47_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1481353.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6772..7674)
                     /gene="fimH"
                     /locus_tag="OQM47_003319"
     CDS             complement(6772..7674)
                     /gene="fimH"
                     /locus_tag="OQM47_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1481354.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7694..8197)
                     /gene="fimG"
                     /locus_tag="OQM47_003320"
     CDS             complement(7694..8197)
                     /gene="fimG"
                     /locus_tag="OQM47_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1481355.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8210..8740)
                     /gene="fimF"
                     /locus_tag="OQM47_003321"
     CDS             complement(8210..8740)
                     /gene="fimF"
                     /locus_tag="OQM47_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1481356.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8750..>10792)
                     /gene="fimD"
                     /locus_tag="OQM47_003322"
     CDS             complement(8750..>10792)
                     /gene="fimD"
                     /locus_tag="OQM47_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1481357.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2585 a         2793 c         2652 g         2762 t
ORIGIN      
        1 ctttagagtg tttcctaccc tttaatatca ctgtgtagag ttgatatcac tacaacagaa
       61 acatgcatgt catttgttta cttctctttt tactacagaa aaatttatct atttttctaa
      121 acatcagtta aatactcata aaaggtaact attatgtcta tgccattaag caatgccgct
      181 aataaccaat tcaatacaaa taatcacttc cttcatcatc caaaagtcga cagtgagtta
      241 acacgtcgat atgaatatgc acgcctggat acggaaaata tctatcttct gcctcttgct
      301 cgtggcaata accataatta cgatggaaaa agcgttgtcg aaattagaaa acttaatatt
      361 ggcaaacagt cctggccatt taattatgtg acagatacat gcagagaatt caatggtatt
      421 acaacaacag gacgtatgcc ctatcgaaac ttgaaaatca cctcagcttt agacgaaata
      481 tatggtggta tatgtaaaaa ggcgcatgct gctacagagt tagcagaagg tttacggcta
      541 aatttattta tgaagagccc tttcgatcct gttgaggact atacagttca tgaaataaca
      601 ttaggccctg gttgcaatgt tcctggttat gccggaacca cgataggata tatttcaacc
      661 cttccagctt ctcaggcaaa aagatggacc aatgagcagc caagaattga tatctatatt
      721 gatcagataa taactgtctc aggggtagca aattcttccg gattcgccct tgccgcatta
      781 cttaatgcta atattgagat gggaaatgac ccaatcattg gtatagaagc ctatcctggt
      841 accgcggaga tccattcaaa aatgggatat aacgttatcc ccggtgatga agatgcgcca
      901 ctcaaacgca tgactctgca accgtcatct cttccagaac tgtttgaatt aaaaaatggt
      961 gaatggaatt atattggtaa atagccactg agaagaatga gtactaatat tcaggttata
     1021 cagatattta aacaaaaccc tgcaatttac aaagtaatac tcattgcgac ttcttaattt
     1081 aagaaagcgc aaggaacgtt tacccttgcg cttattataa taagtcaggc gtcgacttta
     1141 tccagcggcc atgaatcaaa gagatagccg tcaaaataaa tatcgtcaac gttcgagaat
     1201 tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt cgccgctcgg
     1261 gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga
     1321 tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt ttcccgccac
     1381 tgccaggact gacggaataa ctccaccagc atgctgttat gcgttgcttc agcaatggcg
     1441 agatggaact gcatgtcacc gctttcgctg ctacccggcg cgctggaagc cagttcacgc
     1501 tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc ctgcagtgcg
     1561 gcaaactcgg cgatgttact ttccagtaac tgacgcgcct gtaacagctc aaaaggaccc
     1621 gcatcattgc agacgttggc atccggactg tctgtgttgt ggctgcctga gctatcaagc
     1681 acatagatac cggcaccccg gcgcacttcc accagccctt tgatctccag catgatcagc
     1741 gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg ctccggcggc
     1801 agccgttcgc caggattgta cggcgtcttt atgatcagat cgtggatcat cgccccgact
     1861 tcctggtaag gtctttgcgc agaggtgaca gatttcatgg tgttgtgtcc gttaggtgcg
     1921 gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca acgggcaatt
     1981 ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca cgtacaggtg
     2041 ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta agtctgtagg
     2101 gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc acagctgctg
     2161 atatgccgac gtcaccgcgc caacaaagtc ggcattctgc ggcagatcat cggcaaaaat
     2221 accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg cgttaatctt
     2281 ctggaactcg gccagcatcg ggtcgaccac gtcaatggca ttaccctgct catccacgcc
     2341 ctgggtgtaa cccatccagc cagccacgcc cagcgccagg tgacgccagc tgccgccgtt
     2401 ttgcaggtgc agacgcaccg ggtccagtag acgctgcggt aacttctggc tgccgtccat
     2461 cgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga tcagcagcgt
     2521 cgcataggcg ttcaggtccg taccttccgg catcgacagc gttggcgctt gttcctgcat
     2581 catcagggca aaggccgctt tgcgataatc cgggttagtc atggtgtcgg caatggtttc
     2641 atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctgccgttca gcatacgcag
     2701 cttcatcatt tcgaacggca caacgtctgc aacgaactgt gtgcccactt tatcccagtc
     2761 cgggcgacca ttaacgaagt tatcttcaat cacccactga cggaacggtt cgcaggcaat
     2821 ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc
     2881 cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt caatccatgc
     2941 cgctagctgc gggtcacgcg cctgagccag ccccagtacc gcgaccttcg ccacatggcc
     3001 gttttcacgc acgttatcgc aggacatcac cgtaaatgct ttcagccctt tttcacgacg
     3061 cagacgcaag gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag
     3121 atcgtgcttg atcagcgggt tattgagatc cagttgaccg cttgccgcat cagcgcagta
     3181 gcctttttcc gtgaccgtta gagagacaat cgccgtttgc ggacgcgcca tcgcgttgag
     3241 aataccttcg cagccgtcga tttccggatg cagcgcttct ttcatcgaac cgataatttt
     3301 cagctcggtg ctttctgcgc ctttttccgc tacggtgtac agcagttgct gttttttcag
     3361 gttttcgatc agcatgcggt cgttgcctgg catcaggtta acttcgcaga tgccccagtc
     3421 gctgtcggtg ctttccagca gatgatgggt atacagcgcc tggtgcgcgc ggtgaaacgc
     3481 cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc
     3541 aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcagag
     3601 agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg gtattaacgg
     3661 ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag gcctttcaga
     3721 cgaccaattg cggagtaacc agggttggtt ttcttcttca ggtcgtccag catctgatga
     3781 ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct
     3841 tcttcaacaa tcgctttcac cactttgtac atatcaacgt caccgttcag gtgcgccgct
     3901 tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata
     3961 cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac gccgtaggaa
     4021 ccggtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat ccactgcata
     4081 tcttcaatgg tggaaacaat gcgcggcaag ccgaggatcg ggcgcggcgg atcgtccggg
     4141 tgaaccgcca tacgcacgcc aacttcttca gcaactggaa taatcgcttt caggaagacg
     4201 gcaaagtttt cgcgcagttt ggctttgtcg atatctttgt acagctccag gtgcttacgg
     4261 aactggtcga gggtataccc ttcttccgcg cccggcagac cggcaatgat gttacgcgtc
     4321 agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgtt cagctgcctg agcaatttct
     4381 tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc gaatgcagcg
     4441 aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca
     4501 gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccacactgc
     4561 gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt gccagtgtgg
     4621 gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag gcctgcgtct
     4681 tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc gttcgggata
     4741 tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc taaagaaacc
     4801 ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt
     4861 gggttccaca gcaaagagac gcggaacctt ggagttgttt aggtatccgg atgaacaacc
     4921 agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc ttttggtaag
     4981 acatgtcaac cagaattctg aaattggtta accgcgtcgc aagaatttca ctctcatggt
     5041 tgttgctgca aattgcagca aagggtaatc gatggttatt gatcagagga tgtgacattc
     5101 atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa cggttcaacc
     5161 aagtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc ggcattggcc
     5221 tgatgctgat actgaatttg aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg
     5281 cgctgtccgt cgggatgctg gcaggcatgg atttgatgtc gctgctgcac accatgaaag
     5341 cgggcttcgg caacacgctg ggagaactgg ctatcatcgt ggtgttcggt gcggtcatcg
     5401 gtaaattgat ggtcgactcc ggcgcggctc accagatagc acatacgctg ctggcgcgtc
     5461 tcggtctgcg ctatgtacag ctgtcggtga ttatcatcgg ccttattttc ggtctggcga
     5521 tgttctatga agtggccttt atcatgttag cgccgctggt tattgttatt gccgccgaag
     5581 ctaaaattcc gttcctgaaa ctggcgatcc cggcagtagc agctgccact accgcacatt
     5641 cactgttccc accgcagccg ggtccggtgg cgctggtgaa tgcttatggc gcggatatgg
     5701 ggatggttta tatctatggc gtactggtga cgatcccaag tgtaatctgc gcaggtctga
     5761 tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg aaagcagatc
     5821 aaccggtaga tatgaataat ctgccctctt tcggcgtttc gattctggtg ccgctgatcc
     5881 cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa gatacccctg
     5941 cctgggaagt ggttaacttt attggttcct cgccgattgc gatgtttatt gcgatggtgg
     6001 ttgcattcgt actctttggc accgcgcgtg gtcatgacat gcagtgggtg atgaacgctt
     6061 ttgaaagcgc ggtgaagagt attgcaatgg tgattctgat catcggtgcg ggtggcgtgc
     6121 tgaagcagac catcatcgac accggcattg gcgacaccat cggcatgttg atgtcccacg
     6181 gcaatatctc gccctacatt atggcatggc tgatcactgt gctaattcgt ctggcgacgg
     6241 gtcagggtgt cgtttcggcg atgaccgccg ccgggattat cagtgctgca atccttgatc
     6301 cagcaaccgg tcagctggtt ggcgtgaatc cggcgctgct ggtactggcg acggctgcgg
     6361 gttccaacac cctcacccac attaatgatg catctttctg gttgttcaaa ggttactttg
     6421 acctgtcagt aaaagacacg ttgaaaacct ggggtctgct ggagctggtc aactccgtgg
     6481 ttgggctgat tattgtgctg attattagca tggtagcgta aaagaacacc agagcctgcc
     6541 aatggcaggc tcagactgat gactaaagtc taaaaatgtg cccggacagt cccctcgccc
     6601 ctccggggag agggttaggg tgaggggaac agaccagtaa agtgcgcttt gtttatgcca
     6661 gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg ttttgtaggt
     6721 ctgatagcgt agcgcctcag gtaccagcat tagcaatgcg ctgtaatttt tttattgata
     6781 aacaaaagtc acgccaataa tcgattgcac attccctgca gtcacctgcc ctccggtacg
     6841 tgcgtaattt gccgttaatc ccagacttac cgccgaagtt cctactgctc ctaacgatac
     6901 cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc
     6961 tggtgaaaac gacgcggtat tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga
     7021 gaggtaatac cccaggtttt ggcttttcgc acaataaacg gtaagaggaa ttggcactga
     7081 accagggtag tccggcagag taacggtgac atcacgagca gaaacatcac aaccgccagt
     7141 gggcaccacc acatcattat tggcgtaaat attccacaca aactgaaaat catcgctgtt
     7201 atagttgttg gtctgtcgca aaataagcac ggcaattaat gaaccagctt taatagccac
     7261 cccgcccgca ctgctcacag gcgtcaaata aagcgccacc ggccacggct tatccgttct
     7321 cgaattataa acaacccgcg gcgtttcgct ggtggtcgga aatggatagc tactgccact
     7381 atattttacg gtcccggaaa aattagataa cacgccgccg taagccgagc ctcgttgcag
     7441 tgtgacatag tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag
     7501 atctacgacc aggttttgcc ccacattcac gacaggcgca aggtttacat aaacattagc
     7561 gctgccaccg ccaataggga tagcggtacc attggcggtt ttacaggcga atgaccaggc
     7621 atttaccgac cagcccatca gcagtacagc aaacagggta atagctcgtt tcattacaat
     7681 tatctcttcg ggttcagctg taggtatagg tgatgctaat cactgcctga atggttccct
     7741 gagtggctcc gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg
     7801 aggaatcatc cacctgaact gttttggttg cgccagtatt caatgtgttg ccactgtcat
     7861 cctgtagctc taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtgctgt
     7921 cggctgcccc gctgaagctg gcagtgaccc tcgacgttcc caccggacaa ttagtcaact
     7981 caagcgcaac atcatgccag gccgatgccg ccccggcaga cataagactg aaagaataaa
     8041 gatcgccgag atctaccgtg gcattggtgg tggaaaccgt acacggtttg gcgacgacct
     8101 taccgttcac cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg agcgccaaca
     8161 ttgccgccaa tacataccca cgtttgcacc atttcatgag catctccagt tactgatatt
     8221 caagagtgaa ggtagccgta gcattgatat gccccgcagt gacaggcacc tgtgtcgcca
     8281 ttagccgggc gtaaaaattc agcgtatttg gtttacccgg cgtcagggtc gtccacgaaa
     8341 tcgcagacga tggagcatta aggggtattt gattttgctg ctcattcaga agctgtattc
     8401 ccagtcccgc agccgctgac accgtatttt caagtgcaag caggttggca ttgtggctat
     8461 ctgcaacgcc ggtaaaccca acttttacgg cagaaacggc attaccacag ggtgacagca
     8521 aaatacgaaa tggaacgaca ggagtcgtcg cgccaatgtt gttaaattgc ttcgccgcgt
     8581 tttccatcag atcaacagta aaattggttg attcagcggc cacactacag ccgttatctc
     8641 tgacatagcc gcggatagta atcgtgctat ccgcagccaa aacgcgactt accgccagcc
     8701 acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat cacgccccct taacgacatt
     8761 cagctgatag ctgggttaat aactgctgct gactctctgg tggcagttga taattggcga
     8821 cacagtgagc attttcctct tctccccatt tcacctgaac ttttcccgct aaaggcattc
     8881 cgctgaggta aacctgacca ttatccgcaa caatgccgct actctggcta ctctctgatg
     8941 tcaccatcgc cccaaacggc agcggcttat tattgtgagt tagcgtcatg agcagtttta
     9001 tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc acgagtggga acaacgttag
     9061 cgaccgcgtt atctaaatcg acgttatcag ccaaggtatt ggtatccagc gccactctat
     9121 tttcccgata ttcagtggca taaggcagca cggcataacc acgccagtcg gtacgcaccc
     9181 ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc tttaacaagc accaccgtat
     9241 cgtttaacgg ctgccccagc gttacgccat tggcatgagc caatacccca ccgctgactc
     9301 cgtaatagag ctgcttaata tcatcgctat ggctgtaacc gatattggca ttgccgtaac
     9361 caccgcgata attcagcgtg gcgtagcctg tacttccgct attaccatcg cctcccccgg
     9421 catagccggt ttgcacgcta taactgaggt tgttgtcttc cagcaacgta ccgtatacac
     9481 cagccagatt ggtcatccga ccgttgagat cgtgtgacat gctgtagctg gcactggcat
     9541 gtcgccactg agatttactg tcagaacgca gccagtggct gaaaggaata ttgacgttaa
     9601 gcgctaacat ctgatcccgt cctttttgcc aggcgttttt cgtcaggcta tagctgagcg
     9661 tccagttgat atcttcgaac gcagtattta atccagcctg gaattgctca tcgacattac
     9721 tcgttcccca ataagtttga tggctaccac tcaaatacag tgttgatgtg cgcccgagtt
     9781 gctgagtaac ggtgagctgt aatttcccgc gtttgttata agcgaggttg taatagtcgg
     9841 tgaatttcgg cttaacctga ataactccat cctgtgtttc gatgttgtag ccattcattc
     9901 gactgtatgt tgtatcagcg aaattaaaat atccgctggt cgaataacgg taacccacta
     9961 actgaatatt cgtgcctgat tcattgagcg atttgttata gagaaaacgc accgattgtc
    10021 cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc acagacagag
    10081 cgcccagtgc ctccatgttt ttcccgatac cgaaattaaa agcacgataa cgatccgcca
    10141 gttgcgttcc accatatatt gtccagccag ccggaaggcc gtggagtaat gtactctgga
    10201 aaaagcgggg tttttcctgc tgcgcatttc cactacggta ttctcctgcc gtaatggaat
    10261 aacgagtatg cccttcacgt tgcaaaagcg ggactgacga atagggtacg gtaaaaatct
    10321 gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc accactatta cctgcggcat
    10381 agatatcgtt gatggtaaaa ggccccggtg gcaccgtact attataaatg ccatatccat
    10441 tttgtttaat agtgacctgt gcagtaccac gagcaatacc gtggatcacc ggggcaaatc
    10501 ctctttggct atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa
    10561 taccatcgaa aatatcgccc tgggtataac catcacccaa cgtcagccgg gaacgtaaag
    10621 gaattatgtc tcgctcaatc caggtaatga tatgctgcca tttatttttg ctacctgatg
    10681 aactgtcgct actgttataa ctccaggtgg tattgtcgcg taaacgccac gcaccaatat
    10741 ttaacccact ctgtaggttt aaatatgcat aatggctgtt acccccaatc cg
//
LOCUS       DAJVPT010000122        10678 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVPT010000122 DAJVPT010000000
VERSION     DAJVPT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            157..507
                     /locus_tag="OQM47_003323"
     CDS             157..507
                     /locus_tag="OQM47_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1481358.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            560..955
                     /locus_tag="OQM47_003324"
     CDS             560..955
                     /locus_tag="OQM47_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1481359.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            996..1739
                     /gene="cmoA"
                     /locus_tag="OQM47_003325"
     CDS             996..1739
                     /gene="cmoA"
                     /locus_tag="OQM47_003325"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1481360.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1736..2707
                     /gene="cmoB"
                     /locus_tag="OQM47_003326"
     CDS             1736..2707
                     /gene="cmoB"
                     /locus_tag="OQM47_003326"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1481361.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2872..5301)
                     /gene="torZ"
                     /locus_tag="OQM47_003327"
     CDS             complement(2872..5301)
                     /gene="torZ"
                     /locus_tag="OQM47_003327"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1481362.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5326..6426)
                     /locus_tag="OQM47_003328"
     CDS             complement(5326..6426)
                     /locus_tag="OQM47_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1481363.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6814..7560)
                     /gene="cutC"
                     /locus_tag="OQM47_003329"
     CDS             complement(6814..7560)
                     /gene="cutC"
                     /locus_tag="OQM47_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1481364.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7574..8140)
                     /locus_tag="OQM47_003330"
     CDS             complement(7574..8140)
                     /locus_tag="OQM47_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1481365.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8356..10089
                     /gene="argS"
                     /locus_tag="OQM47_003331"
     CDS             8356..10089
                     /gene="argS"
                     /locus_tag="OQM47_003331"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1481366.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10266..>10678
                     /locus_tag="OQM47_003332"
     CDS             10266..>10678
                     /locus_tag="OQM47_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1481367.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQT"
BASE COUNT         2601 a         2733 c         2562 g         2782 t
ORIGIN      
        1 ccataatggg cttttaatcc tatcgtttat tgcttgcata gtagaatcta accatccatc
       61 actcatagct aaccctcgga aatcttacat ttctgtctat cataaccact agcatcacct
      121 ttaggaacca tctgaaacgg gaggtgtatt tagaccttgc taatcttaga cagccgcccg
      181 gttcatgcat cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa
      241 gttccggtac atgctgtact gacggcgaca aatccgctga tccgttttat cggtagtgat
      301 gatatgacgc aaaaccggga attatttcag gtctggttac aaaaattagc gcagtggcat
      361 cagaccacta cgccttatct ttttttacat acgccagata ttgcccaggc cccggaactg
      421 gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct
      481 attccacagc aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc
      541 cattttaaag ggagtttgta tggtaagcgc gctatatgcc gttttaagtg cgttgttatt
      601 aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggtga
      661 cggtggtttt agcgaactgc aaagcgctat ttgcattcat ggtaacgcgg tggaatatat
      721 tcccatcgcg attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt
      781 gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca
      841 ccgtctgttt cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat
      901 ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac
      961 aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt
     1021 ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtcgct gaagtcttcc
     1081 cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt
     1141 tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt tctctgggcg
     1201 cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg
     1261 acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta
     1321 cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg
     1381 tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggca ttactggata
     1441 aaatttatca aggactgaac ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg
     1501 aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa cgtgccaacg
     1561 gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg
     1621 attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat agcgagctgt
     1681 ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat
     1741 cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac
     1801 gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggctgt ttaagcagtg
     1861 gtccaacacg gtggaatttc tgcctgaaat taaaccgtat cgtctggatt tattgcatag
     1921 cgtaaccgcc gaaagcgaag agccactgag caccgggcaa attaaacgca ttgaaacgct
     1981 tatgcggaac ctgatgccat ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga
     2041 taccgaatgg cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac
     2101 cggacgcacc attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg
     2161 cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga
     2221 agcggtgcgt aaactactgg gtaacgatca gcgcgcacat ttgttaccgt taggtattga
     2281 acagcttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttaccatcg
     2341 ccgttcgccg ctggagcatc tctggcagtt gaaagatcaa ctggtgaatg agggtgaact
     2401 ggtgctggaa acgctggtta ttgatggcga tgagaacacg gtgctggtac caggcgatcg
     2461 ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga aaaactggct
     2521 gaagaagtgt ggttttgtag atatccgcat tgtggatgtg tgcgttacca ctactgaaga
     2581 gcagcgacgc actgaatgga tggttaccga atctctgtcc gatttcctcg acccacatga
     2641 tccgagtaag acagttgaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa
     2701 gccgtaaagg tctggtatta ctgccggatg cggcgtgaac gccttatccg gcctacaaac
     2761 ccttgctaat tcaatctatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc
     2821 agttttacgt ttgcataacc tcagcgcccg tttccgggcg ctattcactt cttacgcctg
     2881 aacagcaggc ttatcaaatg ccgttaactt cggtgcgtta cccgtatatt tttcgatata
     2941 caccagcgca gagttaccgg cacaggcatt tgcccgctgc gagctgggga tatccgccgt
     3001 taacacgttc gcactgccgt ttttacacaa gccgttttcc agatctggcc atgcaccttc
     3061 atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg tcaaaatctg
     3121 accacgtttg ttccagacac gcaccagatc gccattcgca ataccaaatc gagcagcatc
     3181 ttcggtgtga atagtgatcg gttcacgatc ggcaaccgca tattttttac gcagttccgc
     3241 atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt
     3301 ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc
     3361 cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc ttggcgtacc
     3421 cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaacgtact gttcgttctt
     3481 ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa acattggcat
     3541 agtgacgcgt tgcgcacggg cccctttctg agcggcatca tagaaaaatt tcagccacgc
     3601 catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttgagta attccgcaag
     3661 atcggcaaac acgtcaaaat cgttacgcgc ttcaaattgc ggagcaacag cctgcttcat
     3721 cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga
     3781 tgtggtgatc ggtaatacga tatctgcgtg tttggctgct gcggtccagt agcattcaga
     3841 aacgacgatc atctccggtt tctgccaggc tttgatcaga cgattagtat cctggtgatg
     3901 ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg tttgttcttt
     3961 accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac gggcaacagg
     4021 aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga
     4081 catttccggc agcacgccgc caacacgcgt cgggttgcca ccattggagt aatgataaga
     4141 gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag
     4201 catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat
     4261 tgtgcgattc gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt
     4321 aatttccgcc gtccagaccg cgctcttcgg cgtattatcg ctcttacctg tcagatactc
     4381 ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac actttatcgt gtttgccttg
     4441 tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca tattcggcgc
     4501 gatccaggtg gcattatcgc caaagaattc gatggtttcg gagcggatag gatcaatggc
     4561 aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt
     4621 actgctccag gcaattttta aggtattcag cgggttcatt ccccacagca ccacaacctg
     4681 gctgttttcc agaatcagcg gccaggaggc ctgctgttca tacacctcta cagaaccgac
     4741 cacatgcggc atgatcacct gtgctgcccc ggttgaataa tcgccgctat gcccggaata
     4801 accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga
     4861 agagcgccag ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt tacgaatacg
     4921 ctcatgttgc tcatgaatca gttttaatgc ctgttcccag ctcacctgta cataggtatc
     4981 ttcgccacga cctttcgccg gttgcagtgg attgtcgaga tagctttttc tcaccatcgg
     5041 atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg
     5101 tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca cttctacgct
     5161 catcgctccc caacgtcccg cggtgaggat tttaccgccc ttctcttctg cccatgcggg
     5221 gagcggtgct gccgatgtca ccaccagcgc tccagcggca ataccgctgt gtttaataaa
     5281 ttcacgtctt gttaatgcca taacttcctc cctgatcaac gaggatcact gtttctcggt
     5341 aatatctttg gcgttgtact ggaaataccg cgttaaaatg tccggttcgt tttcgctcat
     5401 gctggttcgt gcccccattc ctttggcaat ggacggccac gcattgacgg tgtaatggtc
     5461 ggcggcaata gaggcatgac aaccagcgca ataggtatcg tcaagttttt cagcgtattg
     5521 ccatagcggt ttacggtccg ctaatgcggg atcggtaagc gcaccctgta aagacgcctg
     5581 acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtga gcgccttgat
     5641 accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt agagcgtgtt
     5701 ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc cactggcatc
     5761 aacgacggtg agatcggtac caggattcac ggtagccagc tcgcctatgc gagaagtttt
     5821 gaaaggataa atatgtgcgc cattagtcac tgaagtagcg gcctgacttt ccagctcatg
     5881 cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc
     5941 gatacaggtt tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag attcactttg
     6001 cgaggcaata tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc
     6061 ttttaattct ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc
     6121 actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc
     6181 aaataaataa tccatccctg actttggaat atggcaatcg gcacattccg cacgtatccc
     6241 tttctggttc gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg
     6301 gcaggaaagg caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa
     6361 cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc
     6421 tcgcatagat tcctctaaat aaattccaga acaccgaaat cattgtataa ttaaatacac
     6481 aacgaattaa ccgcttgcat aactcggcac aacactgcct gaaataatcg ataaagaata
     6541 tgatttatta caatgtaatc attaattgct aaggaataac ccagttgcta tttagaatat
     6601 atatttatca gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt
     6661 agcacaacgc tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta
     6721 tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc
     6781 atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat
     6841 tcctttcatt tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg
     6901 ttcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc
     6961 tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc
     7021 acggactcct gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat
     7081 ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc
     7141 caattccgca agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg
     7201 aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac
     7261 attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt
     7321 gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg
     7381 tgggcgaatt atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc
     7441 caacgacggc gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc
     7501 gttttgctgc gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat
     7561 ttttgctcct taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat
     7621 gaaacttaat agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat
     7681 gctcaccttt tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag
     7741 aaagcaatgc cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa
     7801 tatgttccca accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg
     7861 aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc
     7921 cattgatcat tttttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct
     7981 cagcggtgac gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat
     8041 ccaaccccag acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg
     8101 caatatcctg cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag
     8161 taaaagcgtt aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc
     8221 tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt
     8281 gcagtatact cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca
     8341 atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg
     8401 attgcggcag gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag
     8461 ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg
     8521 cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt
     8581 gagatcgccg gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat
     8641 gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt
     8701 gtggttgact actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct
     8761 accattattg gtgacacagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc
     8821 gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag
     8881 cagcagcagg aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat
     8941 gcgaaaaagc attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta
     9001 aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc
     9061 atgacgcaga accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg
     9121 atgggcgaaa gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa
     9181 ggtctggcag tagaaagcga aggggcgacc gtcgtattcc ttgatgtgtt taaaaacaag
     9241 gaaggcgaac cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc
     9301 actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat
     9361 tacatcgact cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca
     9421 ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa
     9481 gacggcaaac cgttcaaaac ccgcgcgggt ggtaccgtga aactggcaga cctgctggat
     9541 gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac
     9601 gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc
     9661 aaaaaccgca ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat
     9721 accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa
     9781 attaacgaag agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa
     9841 ctggcagctc gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg
     9901 ccgcatgtaa tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag
     9961 cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca
    10021 caactgacag cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag
    10081 cgtatgtaat cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt
    10141 ttattgccat ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta
    10201 agaggccaga agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata
    10261 ccgaagtgtt taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag
    10321 aagatacggt tatagtaaac gaccatgaca cttcagccat caaagattgt tggcaaaaaa
    10381 attcagatga tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc
    10441 tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc
    10501 cacgcacagg tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc
    10561 gagcctggct ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc
    10621 aggatttaag tgacccctat tctgaatcag aatggctatc atgtatgatc atacagac
//
LOCUS       DAJVPT010000123        10486 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVPT010000123 DAJVPT010000000
VERSION     DAJVPT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(23..1051)
                     /gene="malI"
                     /locus_tag="OQM47_003333"
     CDS             complement(23..1051)
                     /gene="malI"
                     /locus_tag="OQM47_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1481368.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1226..2818
                     /gene="malX"
                     /locus_tag="OQM47_003334"
     CDS             1226..2818
                     /gene="malX"
                     /locus_tag="OQM47_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1481369.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2828..4000
                     /gene="malY"
                     /locus_tag="OQM47_003335"
     CDS             2828..4000
                     /gene="malY"
                     /locus_tag="OQM47_003335"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1481370.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4104..5105
                     /gene="add"
                     /locus_tag="OQM47_003336"
     CDS             4104..5105
                     /gene="add"
                     /locus_tag="OQM47_003336"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1481371.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5141..6181)
                     /locus_tag="OQM47_003337"
     CDS             complement(5141..6181)
                     /locus_tag="OQM47_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1481372.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6424..6549
                     /gene="blr"
                     /locus_tag="OQM47_003338"
     CDS             6424..6549
                     /gene="blr"
                     /locus_tag="OQM47_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1481373.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6822..7037
                     /gene="ydgT"
                     /locus_tag="OQM47_003339"
     CDS             6822..7037
                     /gene="ydgT"
                     /locus_tag="OQM47_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1481374.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7123..7563
                     /locus_tag="OQM47_003340"
     CDS             7123..7563
                     /locus_tag="OQM47_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1481375.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7640..8221
                     /gene="rsxA"
                     /locus_tag="OQM47_003341"
     CDS             7640..8221
                     /gene="rsxA"
                     /locus_tag="OQM47_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1481376.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8221..8799
                     /gene="rsxB"
                     /locus_tag="OQM47_003342"
     CDS             8221..8799
                     /gene="rsxB"
                     /locus_tag="OQM47_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1481377.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8792..>10486
                     /gene="rsxC"
                     /locus_tag="OQM47_003343"
     CDS             8792..>10486
                     /gene="rsxC"
                     /locus_tag="OQM47_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1481378.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2402 a         2708 c         2673 g         2703 t
ORIGIN      
        1 agtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga ataataagat
       61 tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca agtgtgatac
      121 caagttcccg cgctggcgtg ctggcccagg taacaggtat atcatcaagt gtggttggtg
      181 tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg actccgcttt
      241 ccccgctttg ccgccctgct ttcagcaaac caaaccatgc ccccatcgca atagtttcgt
      301 tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg atagcttccg
      361 cggcttgctt ctggctggaa gtgcactcca acacccaatc gctgtgaaac ggcaggccaa
      421 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaacttt
      481 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
      541 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
      601 aagcgaaaat caccgggata gctttttctt ctgccatccg ccgcaggtca tcgctgcttc
      661 ctgcagcccc ggcaattacc acaccatcga caccctgatt cagtaacagt gaaaaccgct
      721 gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt
      781 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
      841 cgatcaaacc aatgacgccg ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca
      901 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc
      961 ctttgccatt aagcaccagc gaaacggtac ttaccgacac gcccgcagcc agcgcaacat
     1021 catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg attatgtgac
     1081 agataaaacg ttttaccttt tatttatctt atacccgcta ttatcgttgc gtaatgtgat
     1141 ttatgactca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta ctgaatccag
     1201 attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg
     1261 ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat tgtcgttctg
     1321 cggcattatg ctcggcattg gtagttctct tagcagccat gatgtcataa ccctgatccc
     1381 ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga ttggctcgtt
     1441 tgcttttagt ttcctgcctg tcatgttctg tatcgccatc ccgctgggcc tggcacgcga
     1501 aaataaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga acctcgcggt
     1561 aaacttctgg ttgaccaata aaggcattct gccaaccaca gatgccgccg ttctgaaagc
     1621 caataacatc cagagcattc ttgggatcca gtcgatcgat accgggatcc tcggtgcggt
     1681 gatcgccggt attatcgtct ggatgctgca tgagcgtttc cataatatcc gcctgccgga
     1741 tgcgctggca ttcttcggcg gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg
     1801 ccttgtcggc ctggtgattc cattagtctg gccgattttc gccatgggta ttagcggctt
     1861 gggccatatg ataaacagcg cgggtgattt cggaccgatg ctgtttggta ccggtgaacg
     1921 tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct ttaccgacgc
     1981 aggcggcacg caggaggtct gcggtcaaac cgtcagcggc gcactgacca tcttccaggc
     2041 gcaattgagt tgcccgacca ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca
     2101 aggtaaaatg cctgcgtttc tcggcggtct accaggtgca gcgttagcta tgtatcactg
     2161 cgcgcgcccg gaaaatcgcc ataaaattaa aggtctgctg atttctggcc tgatcgcctg
     2221 cgtcgttggc ggcactaccg aaccgctgga attcctgttc ctgttcgtag cgccagttct
     2281 gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg tgctcggcgt
     2341 caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg
     2401 tctgtcaacc aagtggtaca tggtgccagt ggtggcggca atctggtttg tcgtttacta
     2461 cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc gcgatagcga
     2521 agttgccagc tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt
     2581 tcctgcaatc ctcgaagcat taggcggtgc cgacaatatt gtcagcctcg ataactgcat
     2641 tacccgtctg cgtttgtctg tggaagatat gtcgcttgtt aatgtgcagg cactgaagga
     2701 caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg ttatcgggcc
     2761 acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc aggcataagg
     2821 ataagatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg
     2881 ggattatgtc gctgaccgtt tcggcactgc tgacctgtta ccgttcacga tttcagacat
     2941 ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga tgcacggcgt
     3001 atttggctac agccgctgga aaaacgatga gtttctcgcg gctattgccc actggttttc
     3061 cacccagcat tacaccgcaa ttgatacgca gacggtggtg tatggccctt ctgtcatcta
     3121 tatggtttca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac
     3181 gcccgcctat gacgcatttt acaaggccat tgaaggtaac cagcgcacag taatgcccgt
     3241 tgctttagag aagcaggctg acggctggtt ttgcgatatg ggcaagctgg aagccgtttt
     3301 ggcgaaacca gaatgtaaaa ttatgctcct gtgtaaccca cagaatccta ccgggaaagt
     3361 gtggacgtgc gatgagctgg agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt
     3421 tatttccgat gaaatccata tggatatggt ctggggcgag cagccgcata ttccctggag
     3481 taatgtggcg cggggagact gggcgttgct gacatcggga tcgaaaagtt tcaatattcc
     3541 cgcgctgacc ggtgcttacg ggattataga aaatagcagc agccgtgatg cctatttatc
     3601 ggcactgaaa ggacgtgatg ggctttcttc cccttcggta ctggcgttaa ctgcccatat
     3661 cgccgcctat cagcaaggtg caccgtggct ggatgcctta cgcgtctatc tgaaagataa
     3721 cctgacgtat atctcagata aaatgaacgc cgcgtttcct gaactcaact ggcagatccc
     3781 gcaatccact tatctggcct ggcttgatct acgtccgttg aatattgacg acaacgcgtt
     3841 gcaaaaagcg cttatcgagc aagaaaaagt cgcgatcatg ccggggtata cctatggtga
     3901 agaaggtcgt ggttttgtcc gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg
     3961 tgtggctgga ttaataaacg ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa
     4021 aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt gcgcactttt
     4081 atatccagaa aaagagtgcg accatgattg ataccaccct gccattaact gatatccatc
     4141 gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc cagtataata
     4201 tctcgcttcc tgcacaatcc cttgaaacac tgattcccca cgttcaggtc attgccaacg
     4261 aacccgatct ggtgagcttt ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc
     4321 ttgatgcctg ccgccgcgtg gcatttgaaa acattgaaga tgcagcccgt aacggcctgc
     4381 gctatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacataag ctgcctgtag
     4441 cgggtgttgt cgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc
     4501 aggcgaagct tatcggcatt atgagccgaa ccttcggcga agccgcctgt cagcaagagc
     4561 tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagct ggtgatgaac
     4621 ttggattccc gggaagtctg ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc
     4681 atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag gcgattcgtg
     4741 aactgggtgc ggagcgtatt ggacatggcg taaaagccat tgaagatcgg gcgctgatgg
     4801 attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat attcagacca
     4861 gcaccgtagc agagctggct gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg
     4921 ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac gaatataccg
     4981 ttgccgcgcc agctgctggg ttatcccgtg agcaaatccg ccaggcacag attaatggtc
     5041 tggaaatggc tttcctcagc gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga
     5101 agtaacaaaa atggatggtg caaattgcac catccatttt tcacgcaagg cacaacgtcg
     5161 cgcgatgttt ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc
     5221 ttgccggaac cggattttca ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag
     5281 ccggataatt cccaggcacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc
     5341 gggtaagtac gccatcacgc atatcgtagc cccagtcttc ctgcggcaga cgctcgccat
     5401 ttttcagacg ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat
     5461 gcacgatata ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc
     5521 gctgtggata ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact
     5581 gtgccaaatc taccgtcatg ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa
     5641 gatgcggtgc taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat
     5701 cgcgcacctg cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca
     5761 cgccttccgc gagcaaacct tttagcgtca agaaatcgct atcccaacgg cggttatgga
     5821 atacagacag cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca
     5881 gtgtcacggt aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg
     5941 ctaacgggaa atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt
     6001 taaacagatg cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg
     6061 tttcatcact gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg
     6121 catggaaggt tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca
     6181 tgtcattgcc tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa
     6241 cagtttaata acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga
     6301 attttccaac gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta
     6361 ttattgctaa gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct
     6421 gtaatgaatc gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt
     6481 cttggcgtgg cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa
     6541 cacaagtaag ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact
     6601 tgtcggatat taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga
     6661 tagtaaagcg cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg
     6721 cattcttatt attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat
     6781 aatacctacc cctttggtat taattatttt atggaccttt tatgactgtt caggactact
     6841 tattaaaatt tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta
     6901 attacaccct gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc
     6961 gcgcagagct ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct
     7021 ggcactatgt ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct
     7081 aaaattagat ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca
     7141 caacgtattg gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt
     7201 acgacgctgg cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg
     7261 ctcggcggac aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc
     7321 gccatgtggt attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt
     7381 cctaaacact atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct
     7441 ttttcacccg tggaagttgg catagccgtt cgccagttgc tgtttacttt gttggcaacc
     7501 gcattgatcg tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg
     7561 taataacctt acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt
     7621 ttttatttca ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt
     7681 caataacttt gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa
     7741 actggaaacc gcgatgggca tggggctggc aacaacgttt gtgatgacgc tgacgtctat
     7801 ttgcgcctgg cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac
     7861 cctggcattt attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa
     7921 aaccagcccg gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg
     7981 tgcagtgctc ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc
     8041 gctgtacggt ttttccgccg ctgtcggttt ctcgctggtg atggtgctct tcgccgccat
     8101 ccgcgaacgc cttgctgtgg ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt
     8161 aattaccgca ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta
     8221 atgaatgcta tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc
     8281 attctgggtt atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt
     8341 gacgaaatct taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac
     8401 gcggaagcca tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct
     8461 gtgatgctaa aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg
     8521 caagagctaa cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc
     8581 actaaatgta ttcaggcttg tccggtagac gccatcgttg gcgctacccg tgtcatgcat
     8641 acggtaatga gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac
     8701 tgcatctcgt tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc
     8761 attcccgtgc gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa
     8821 aaataaaatc tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagaccaa
     8881 cggtaccccc ctgcgccagg tactactggc gcagcatttt gttattccac tgaaacagca
     8941 tattggcgct gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc
     9001 gcttacccgt ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac
     9061 ggctattgcg ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat
     9121 tgatgccgat ggtgaagact gctggatccc gcgcgacggc tgggtcgatt atcgctctcg
     9181 cagtcgcgaa gagttaatcg agcgcataca tcagtttggt gttgccgggc tgggcggtgc
     9241 cggattcccg acaggcgtta aattgcaggg tggtggagat aagattgaaa cgttgattat
     9301 caacgctgct gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc
     9361 ggctcaggtc gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct
     9421 tatcggcatt gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga
     9481 ctctaacgat atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca
     9541 attaacctac attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg
     9601 cgtattaatg caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga
     9661 gccgattacc gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt
     9721 ctgggcacgg ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc
     9781 tgccgatcaa atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga
     9841 tgtcccggtc gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga
     9901 accacaggaa gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgtgga
     9961 tcttttgccg caacagttgt actggttcag caaaggtcag caacacgata aagctaccac
    10021 gcataacatt gccgactgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat
    10081 tcccttggtg caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga
    10141 aaagcgcgcc gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga
    10201 aaaagcgact cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa
    10261 agatgcgatt gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc
    10321 tattgtgatt aaagcgggcg aacgcccgga taacagtgca attattgcag aacgggaagc
    10381 ccgtaaagcg caagccagag cgaaacaggc agaactggag caaactaacg acgcagcaac
    10441 cgttgctgaa ccacgtaaaa ctgccgttga agcagctatc gcccgc
//
LOCUS       DAJVPT010000124         9996 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVPT010000124 DAJVPT010000000
VERSION     DAJVPT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1270
                     /locus_tag="OQM47_003344"
     CDS             <1..1270
                     /locus_tag="OQM47_003344"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1481379.1"
                     /translation="GSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQ
                     DYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYH
                     PTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGCSQIL
                     DPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLVNIGV
                     GPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAVLPPD
                     IPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASALRFNL
                     SRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAISEHLQ
                     IGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1613..2329
                     /locus_tag="OQM47_003345"
     CDS             1613..2329
                     /locus_tag="OQM47_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1481380.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2785..2860)
                     /locus_tag="OQM47_003346"
     tRNA            complement(2785..2860)
                     /locus_tag="OQM47_003346"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2825..2827),aa:Asn,seq:gtt)
     gene            complement(2961..4588)
                     /gene="yeeO"
                     /locus_tag="OQM47_003347"
                     /pseudo
     CDS             complement(2961..4588)
                     /gene="yeeO"
                     /locus_tag="OQM47_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4593..4668
                     /locus_tag="OQM47_003348"
     tRNA            4593..4668
                     /locus_tag="OQM47_003348"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4626..4628,aa:Asn,seq:gtt)
     gene            complement(4706..5656)
                     /gene="cbl"
                     /locus_tag="OQM47_003349"
     CDS             complement(4706..5656)
                     /gene="cbl"
                     /locus_tag="OQM47_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1481381.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5758..6675)
                     /gene="nac"
                     /locus_tag="OQM47_003350"
     CDS             complement(5758..6675)
                     /gene="nac"
                     /locus_tag="OQM47_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1481382.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7003..7078
                     /locus_tag="OQM47_003351"
     tRNA            7003..7078
                     /locus_tag="OQM47_003351"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7036..7038,aa:Asn,seq:gtt)
     gene            complement(7134..8069)
                     /gene="ldtA"
                     /locus_tag="OQM47_003352"
     CDS             complement(7134..8069)
                     /gene="ldtA"
                     /locus_tag="OQM47_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1481383.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8131..9210)
                     /gene="cobT"
                     /locus_tag="OQM47_003353"
     CDS             complement(8131..9210)
                     /gene="cobT"
                     /locus_tag="OQM47_003353"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1481384.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9222..9965)
                     /gene="cobS"
                     /locus_tag="OQM47_003354"
     CDS             complement(9222..9965)
                     /gene="cobS"
                     /locus_tag="OQM47_003354"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1481385.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
BASE COUNT         2809 a         2536 c         2159 g         2492 t
ORIGIN      
        1 cggtagcaca acaaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
       61 ctacaccacc acgattactc gccctactct ctttcgttcg tatctgaatg agcaactaac
      121 gttgctttat caggattatg gtgcgcatat ctctgtgcaa ccctcgcaac atgaaatccc
      181 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgattt
      241 aactcgctac ttcccgacaa cagaactggc gcaaatcggc gatgaaactg cagacggcat
      301 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      361 cctcgcacgg ttgcgccatt ataccggtac gccagtagaa cattttcaac cgttcgtctt
      421 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
      481 cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga tcactgccga
      541 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
      601 atggcattta attaccgctg atggtcaggg tattactctg gtgaatattg gcgtgggacc
      661 gtccaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggata tctggttgat
      721 gattggtcac tgtggcggat tacgtgaaag ccaggccatt ggagattatg tccttgcaca
      781 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata tacctattcc
      841 gagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca gcggcaggcc
      901 tggtgaggaa gtcaaacagc ggctgcgtac aggtactgtg gtaaccacag atgacaggaa
      961 ctgggagtta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1021 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1081 actactgtgt gtttcagata aaccgttaca tggtgagatt aagcttcccg gccaggctaa
     1141 ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg cgatcgattt
     1201 gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta atgagccgcc
     1261 gttccgataa taagttatga accttcttat cagaaataga tgtgaaggat taagtaagac
     1321 atctggcaaa tagcctgcaa taaacgcggg ctgcttgctt ccttgggcag atgcgagttt
     1381 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     1441 aaaaaatccc gccaacaaca ttagttttta taaaattaaa tataagatta tggctttaga
     1501 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     1561 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     1621 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     1681 tgcaaaattc ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg atccagaatt
     1741 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     1801 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tacagggacg
     1861 ttatgaaggc tttggtccta atggctcaat gattattgct gagacattga cttcaaatgt
     1921 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     1981 ggcaggttct gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggaacaga
     2041 ccctgaccat atttttgaaa ttttgcttga agctgaagtt gatgttcgtg atgtaactga
     2101 agaagaaggt aatattgtta tttatactga acctgctgac cttcataaag gaatcgcggc
     2161 gctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2221 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2281 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatattttaa
     2341 agaaatctgt ctttatggca gatttcttta atctcatata attcttataa aaaatataat
     2401 atttaactcg tcatattgct tataaaaaat ccccgtcccc agagaaataa tatttattaa
     2461 aatggcaggt cttctttttc tgattaccga agacaaagtg gcaattacgt atagtttccc
     2521 gataaagacg cgacagctac gtcccgcata aggcattttt ctctttatct ttgtacggta
     2581 cttcatggaa cagaattttt gaccttgtga atcgtgatat ctgttgggga ggggcaattt
     2641 gctcactgaa gcgtgagact cgattaagcg cacgaaacac agaaatcaaa aaacccggcc
     2701 acttttttac aaagtggccg ggtaaaaata atttttattt tttaactgtc ctgagtctca
     2761 tagagatgtc tcaaaactaa aatttggctc ctctgactgg actcgaacca gtgacatacg
     2821 gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgagaa cgaggcgaat
     2881 attagcgatg cccacccaca atgtcaaagc ctgtttttta aatttgaaat cgtttgctga
     2941 aataatctgc attttgtcgt ttattccgac acaactggct ttttttcaca cttttgcggc
     3001 tcggatcgag ggtatttcca cagccaacgt ccagtaacca ttcgccagta aaacagcacg
     3061 gcccgcacag cccagtcggc aaacattccc atccagacac caaccacacc ccagccaagc
     3121 atgattccct gcacataacc gactacaacc cggcaacccc acatgctcaa catagaaacc
     3181 cacatggcgt aacgggcatc acgagcaccc ttaaatccag cgggtagcac ccatgaggcg
     3241 gaccaaatag gcataaataa agcatttagc taaatcagaa tcacaacgac gtgtttaacc
     3301 tgtggatcct gggtgtaaaa cgatgccata accccggcaa agggagccgt tagccaggcg
     3361 atggccgtta atccaagagt ggacagccag aacacatgcc gcaactgaat ctctgcttgc
     3421 gctatctgcc ctacccccaa ccttcggcct gtaatgatcg tagaagcaga accgagcgca
     3481 cttccgggta agttgataag agccgcaatt gagaacgcga taaaatttcc ggcaataacg
     3541 ctggtcccca tcccggcaac gaacatttgg gttaataacc gaccactggt aaataacact
     3601 gattcgacac tcgctggaat accaatcccc atgacttccc agataatgct aaaattcagc
     3661 ggtttaaaat agctctttaa cgaaatcctt agcgcaggat taaaaccaat cgccagcacc
     3721 cacaaaattg caactgcgcc aatataacga gaaatggtta aacccaaccc agccccgacc
     3781 aggagaaaag gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac
     3841 cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa
     3901 gagtgatggc agcggctgga taactgagta cggtcagctc cagataagtc aacgccagtg
     3961 ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat
     4021 gaattagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg
     4081 ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgctacaacg acagtagtac
     4141 caagatcgat agctgcaaaa aaagccataa tgaccatatt gaagctgtcc gccaacccca
     4201 cgccggccat cgcatctttt cccagccaac tgaccagaaa agtgctcagg acccccatca
     4261 acaggacaca ggcgttctcc atgaagatag gaacagcaag cggggttatc tcgcgctaga
     4321 acaacacttt gtagctcttg cgtttagagt gccagcgagt gccgtgaaca acctggcgta
     4381 aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaatg
     4441 atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac
     4501 aaactgttga tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaatat
     4561 ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc
     4621 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa
     4681 gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt
     4741 ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc
     4801 gccaaacata gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat
     4861 cgaaaagatg ccgcgtatcc aggcggatta aattctcttc ctcttgttcg ccgctggatt
     4921 gttcggcaac taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat
     4981 cctgtgcact taatacaata tctgccggca aacctttgcg ggtaaatgcg tcatcagtgc
     5041 gtgagcgccc cgtaattccc tgtcggtaag taattaacgg ccacttcgct attgattcca
     5101 gcgtcaatgg tgtaatttgc gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc
     5161 aacaaaacca tgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac
     5221 caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgaa ccctggatta
     5281 gctcgagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat
     5341 aacgtgcctg agtatgcgtc gttgcaatag tgagaacacc agacgtatcg ttggtaaaca
     5401 ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta
     5461 atgctttgcc cggttcagtc atccccagca gtcgcttacc tcgtcgaaca aatatttcga
     5521 tgccaaattc atcctccagt tcccgaatat gacggctaac gcctgactgt gaggtaaaaa
     5581 gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta
     5641 gttgttggaa attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct
     5701 aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta
     5761 gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa
     5821 ctctttcact gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa
     5881 agagagactc attgaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa
     5941 cgaacgcgca gccgattcag gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt
     6001 aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa
     6061 agcctcatca acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg
     6121 cgcaatagca ttaacatcaa cgttttgccc cgggcaatct tgagttccta ccaggaaaag
     6181 atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac
     6241 cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccac tattttcatg
     6301 aagatagata acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat
     6361 ggatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgactgacc
     6421 aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc gtgcatgggt
     6481 atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg
     6541 ttgatttaac tcaccttcca gtatggcaac ctgttggctg agcgctggtt gtgcgatatg
     6601 caatacttca gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag
     6661 gcgtctgaag ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt
     6721 acaaccagta tgcaagatgc ctgccagttc tattttggcg ttgatgcgca agctaaccaa
     6781 ctgtcaaata agagattatg atagattcgt cattggccct ttttatcagc agtcgcattc
     6841 acctgcccta taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag
     6901 ctcaccactt tgttgaaatt ggcagcaaac aaacaaaaaa aatgcatttc accctttgac
     6961 atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt agttcagtcg
     7021 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     7081 ttcaaaaaag cctgctttcg agcaggcttt ttactttcta attaccaacg ctcttaaaac
     7141 atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgatgttg caacgcagca
     7201 tttgcgcgat ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga tggggttacc
     7261 ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag cggctcatga
     7321 acttccagcc actttgaacc atccggttcc gttgtgtatt tcactggctg atcaataatt
     7381 tgtacacgag tgcctacagg aacattatca aacagatatt tgatatcgtc attacgcaga
     7441 cgaatacagc cctgacttac acggagccca ataccaaaat tggcattggt accgtggatg
     7501 gcatacaacc tgccaatata aatcgcgtac agccccatgg ggttatcggg ccccgcagga
     7561 acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccggatgtt cggcgttggc
     7621 gtccaggttg gtgcttcttg tttacgttca acggtagtca cccagttacg cggggtttct
     7681 cgcccagcct ggccgatacc aataggaaag acttccactg tattactgtc tggcggatag
     7741 taataaagac gcatttcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa
     7801 atcagttgct gcggaatggt gagttgtgag ccaggcttcg gcaaaaaaac atcagcgccc
     7861 ggattcgctt ccagcatatt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc
     7921 ggctgagtat tgtgatcagg aacagttaca gtaagcgact gccccactaa acggctaccc
     7981 tctggaggta atggataagt taccgccagg ctattttggc tggcaaaaag cagagcaaat
     8041 gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa gactatccga
     8101 taagtatagc ttttatcaga cttttcgttt ttagctgttc aaatcagaag tcgtattccc
     8161 cggtagaaca atattactgg cagcaagttc gcccatgttg ttgtatatcg cacaagcagc
     8221 ttcgatgatg gacatcgcca gagctgcacc actcccctca cctaaacgca tatccatatt
     8281 gagataaggc tccagcccca aatgcgagag cgctatacgc gcgccttttt ctgccgacaa
     8341 gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcagc
     8401 agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag cacctaacat
     8461 cactccagct attccgacca aatcaaatcc acccactttt gccaggacat caacaccatc
     8521 ctgaggattt ggttgattca acgtaatcgc ccgacgcaca acatcaattt tattagccag
     8581 tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc ggccagtgat
     8641 tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa
     8701 cagcgtgaca ccgtttttcg ccagctcccg cgtataacat atgacgtcca aaagcaactt
     8761 ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac
     8821 acgcatgttg ataagcccgg ggataggctc agcagtatca ataccaacat caattacgtg
     8881 gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccac gggtcatatt
     8941 ttcagcctgt atggctgtca cttcttttgg agaaatagcg accccttcct cccagacgcc
     9001 gtgatcggca cacataacca gtaccgcttt tttgcccaca tgcggtatgc cattcacccc
     9061 cggcattcct gccagttgta tggcaagcgc ctccagcttt cctaggctac caacaggttt
     9121 gaataacccg tcaacatgcc gttgtgcacg cgacatagcg gcagaattga tggctgggat
     9181 cgtattcagt aaatcggcaa ttatttgcat gtcacgtttc tttatagcag tgccagcagg
     9241 aagaccagtt caccaagctc tatggctgct cccagcgtat cgcccgtttg cccgcccagc
     9301 gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc
     9361 acaccatgca tacccagcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg
     9421 caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc ttcccgcgca
     9481 taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc
     9541 agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaacaccag aatctttgcc
     9601 agtaccacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt
     9661 tccaacatgc gatcgcgact acgtgcagaa aatacgccat cacaggtatc agcgaggccg
     9721 tccagatgga attccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt
     9781 ggtgcgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat
     9841 aatccaatca aaggaaaagt aataataccg cgagaataat gttcgaaatc cagtccctgg
     9901 gaccagcgac gcggtacagg cagacgcgta ataaatgaga gcatcgccca aaataatttg
     9961 ctcatttgat ttttactcca atacccgaaa ccacca
//
LOCUS       DAJVPT010000125         9766 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVPT010000125 DAJVPT010000000
VERSION     DAJVPT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..948)
                     /gene="bglB"
                     /locus_tag="OQM47_003355"
     CDS             complement(<1..948)
                     /gene="bglB"
                     /locus_tag="OQM47_003355"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1481386.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQ"
     gene            complement(967..2844)
                     /gene="bglF"
                     /locus_tag="OQM47_003356"
     CDS             complement(967..2844)
                     /gene="bglF"
                     /locus_tag="OQM47_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1481387.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2978..3799)
                     /gene="bglG"
                     /locus_tag="OQM47_003357"
     CDS             complement(2978..3799)
                     /gene="bglG"
                     /locus_tag="OQM47_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1481388.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4086..4811)
                     /gene="phoU"
                     /locus_tag="OQM47_003358"
     CDS             complement(4086..4811)
                     /gene="phoU"
                     /locus_tag="OQM47_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1481389.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4826..5599)
                     /gene="pstB"
                     /locus_tag="OQM47_003359"
     CDS             complement(4826..5599)
                     /gene="pstB"
                     /locus_tag="OQM47_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1481390.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5691..6581)
                     /gene="pstA"
                     /locus_tag="OQM47_003360"
     CDS             complement(5691..6581)
                     /gene="pstA"
                     /locus_tag="OQM47_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1481391.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6581..7540)
                     /gene="pstC"
                     /locus_tag="OQM47_003361"
     CDS             complement(6581..7540)
                     /gene="pstC"
                     /locus_tag="OQM47_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1481392.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7627..8667)
                     /gene="pstS"
                     /locus_tag="OQM47_003362"
     CDS             complement(7627..8667)
                     /gene="pstS"
                     /locus_tag="OQM47_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1481393.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8915..>9766)
                     /locus_tag="OQM47_003363"
     CDS             complement(8915..>9766)
                     /locus_tag="OQM47_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1481394.1"
                     /translation="DTGNVGEFPYIYYTSKINQALTYAGVHSNINYYDLNPNLDVGIA
                     IDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKKTFAGKLII
                     PETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINNLKPVCFDFKEIPAAD
                     FSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGI
                     KIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATAT
                     ITVEIVR"
BASE COUNT         2518 a         2546 c         2260 g         2442 t
ORIGIN      
        1 ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt aataactaaa
       61 agagatgaaa tctacagtat gttttaaatc ttctgcgtca ctttcagtca tctcaatggt
      121 gatattgtgg tcgcggaaga aacgctgcat atagccggga tattggccac gcgcctgaac
      181 atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat cctgtggctg
      241 gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg cttcggggat
      301 caggctatga taggctttaa ctgcccgcgc actggcaacc agttgatgat ggatcgcctg
      361 ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg gcgcgtgtaa
      421 cgacatattg atttcattaa acgtcagcca taacgctact ttatgttggt agcgggtaaa
      481 gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc aaccgccgta
      541 gtttttcacc agcccatata gcatttcgta atgggataac gttaccagcg gcttgatccc
      601 cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt cattcggttc
      661 ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa
      721 gcccatctcg gcaaataacg cgatatcttc cgggtaacgg taataaaaat cgatggcgac
      781 atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta cgccatgagg
      841 ctgtaaatct gaggtcgtga tccctttgcc atcttccagc caggcacctt ccacctgatt
      901 ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcataa ttaactcctt
      961 ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg cttatctgcg ccgtgccgtg
     1021 gggtaatacg tctgtaaaat catcgctatt actgattaat accggcgtcg tcagatcaaa
     1081 tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg tattgacctt
     1141 gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc
     1201 gacatgaatc aggatctcca caccatcatc tgactcaatg ccaatggcgt gtaatgtggc
     1261 gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg agggcagaat
     1321 ggcgatacct ttacccaaca ggccactggc aaacgtggta tcagcgacgt gaatgagcgg
     1381 cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg taataacctc
     1441 tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttactggtga tgaaatgaag
     1501 catcaacgta ccaacaaatg cgcaacctat ggcaatgaca ccgccaataa cgctggccca
     1561 gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg aaggtgaaaa tacttggcaa
     1621 accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca atgctccact
     1681 gatacaggcg ataacaaagg ggtacttacg cggcaggttg acgccatata ccgctggttc
     1741 ggtgatacca aacagacccg tcaacgccgc tgatcccgcc accacttttt tctgcgcatc
     1801 gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg cgggcattaa
     1861 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca gcggcaccag
     1921 gcccctgtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta ccgcgcccgc
     1981 aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcattt cgcttatcca
     2041 ggttgatagc tgccccacca gcagaaaggt gacgggtgtg ataaccatca gacatagcaa
     2101 tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc gttccagaat
     2161 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
     2221 gaccggaata cccaggaaat ccagccccgg cgcatccgct ttttgcccgt tctcgaaggc
     2281 agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa atggattacc
     2341 gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg aagaacccaa acaaggcatc
     2401 actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact gaaaagtgag
     2461 cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcagaccga tcagaggcgt
     2521 aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct catcattttc
     2581 cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca ggaagacatc
     2641 ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca ccataataat
     2701 accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta acttaaaacg
     2761 taatcgcgtt gcgcaatgca tcagactcac aatgttatct gcgcccccga ctcctgcgac
     2821 tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactccaaa
     2881 aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg ttttgcccgc
     2941 ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg cgcacgcgct
     3001 ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga tactgcaaac
     3061 caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt tgttttactg
     3121 cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata cgccaggata
     3181 aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg taattaaggc
     3241 tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca cctgcaacat
     3301 cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag cccacttcat
     3361 ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct tcccctagct
     3421 ggaactcttt cgggtaaagc cgctggatat cccacagcaa cacgttttgc tgaaagcgtt
     3481 taatcgcaaa ctggcaatgg tcagttagcg agatataaat actgtcctgt aattttccca
     3541 agcgctcctg cgctaaagag ataagacgat cacaggttgc catcacctca agaggaatat
     3601 gacttaagag ttcgcttaat cgcccgttca gttcatgact gctcaaggca tactcttttt
     3661 ctattccact tgagttaatt ctttcgccag cgcgtttttg aaagccaatt ccgcgcccca
     3721 tgacgacttt ttcccgctgt tgatcatcaa taaccaccac aacattattg ttgagaattt
     3781 tggtgatttg catgttcata gcaaggacct tttttataaa caaaaaaaaa ccgacttcac
     3841 cagtattctc tggttatgtc aggttttgcc tgcgaatgca gtaacaatcc agtcatttat
     3901 taatggtttt tataacgaac atccaggttc ggaaattaat ttaattgcgt gcagagaaaa
     3961 tagcaatgcg ctattgataa aaatatgacc atgctcgcag ttattaactt tgtgtaattt
     4021 taggaattta taaagttata tataacaaat cccaataatt aagttattgg gatttgtctg
     4081 gtgaattatt tgtcgctatc ttttcccgcc agcagtttat ccagctcatc accgcctaca
     4141 tgacggaaat cctgaccctt cacgtagtag aagataaact cacaaatatt ctggcagcgg
     4201 tcgccgatac gttcgattga acgcgcacag aacagcgcgg taagtacgct cggaatagtg
     4261 cgcgaatctt ccatcatgta ggtcatcagt tgacgaacaa taccttcgta ttcctgatcg
     4321 acttttttat cttcacgata aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg
     4381 tccagcacgt cgtgcagcat ctggatggta tgacggccca gcgactccag acttaccagc
     4441 aacggctgat gctgctggga gaatttctcc agcgcagtac ggcagatttt gtccgccacg
     4501 tcgccaatac gctccagctc ggcaatggtt ttactgatca ccataaccag tcgcaggtcg
     4561 ctcgccgtcg gctgacgttt ggcgataatg cgcacgcagg cttcatcgat cgccacttcc
     4621 atcatgttga cgttcttgtc gccttcgatc acgcgcttcg ccagatcgct gtcctggtta
     4681 tgcatcgcgg tgattgcatc agaaagctgc tgctccacca tgccgcccat ggtcatcacc
     4741 tgcgtgcgga tactttccag ttcggcgttg aactggccgg aaatatgttt attaagattg
     4801 agactgtcca taacgcactc ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt
     4861 gtttcttcgc tggcttggtg aacagatcgt ccgtgttgct gaactcaatc aattcgccca
     4921 ggtacataaa tgccgtgtgg tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga
     4981 tcaccacggt gtaatcctgc ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga
     5041 tggggtcgag cgccgaacac ggttcgtcga gcagcagcac ttccgggcga atggcgatac
     5101 cacgcgcaat acacagacgc tgttgctgac caccagagag agagtaaccg ctctggtgca
     5161 atttatcttt ggtttcgttc cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt
     5221 ccatgtcggc acgggagagc ttctcaaaca gacgaacgcc aaaggcgatg ttgtcgtaga
     5281 tggacatcgg gaacggcgtc ggtttctgga acaccatgcc cactttcgca cgcagcaggg
     5341 cgatatcctg agagttggtc aggatgttgt cgccatcaag cagaatttca ccttccgcac
     5401 gctgctccgg gtacagttca aacattttgt tgaaggtacg cagcagcgtc gatttaccgc
     5461 agccggacgg cccgataaac gccgtaacct ggtttttggc gatatccagg ttgatgtttt
     5521 tcagggcatg gaatttgccg tagtagaagt tcaaattacg aacctgaatt ttactcgggg
     5581 cagtttcaac catactcatt gcaatctttt cctcatcgta cctgatgctt ttcgctaatc
     5641 aggcttaccg tagggccggg taaggcgttc acgccgcatc cggaaaagtg ttaaccgtgt
     5701 ttattcttcg caaaaacaac gcgcgccaga atgttcagca gcaggacgca cagggtaatg
     5761 atcaataccc cggcccaggc cagttgttgc cattccgcaa acgggctcat cgcaaactta
     5821 aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt ccgtgctcca gaactggttg
     5881 gagagcgcgg taaacagcag cggcgcggtt tcaccggcaa tacgggcaat cgccagcagg
     5941 ataccggtca taatcccgga caccgacgct ttcagcgtaa tcgcggagat catcttccac
     6001 ttcggtgtac ccagcgcata agccgcttca cgcaggctgt ccggcaccag tttcagcatg
     6061 ttctcagtgg tgcggataac gatcggcacc tgcaacagcg ccagcgcaat tacgcccgcc
     6121 cagccggaga agtgctccat ctgtgccacc acgatggtgt agacaaacag accaaccaca
     6181 atcgacggcg cggagagcag aatgtcgtta atgaagcgaa tcacttctgc cagccaggat
     6241 ttacgaccat attccgccag ataaatcccc gccataatgc ccagcggcgt accgaatacc
     6301 gtggcccaca aaattaacag cccgctaccc gccagggcgt tcgccagacc accaccttcc
     6361 gtattgggcg gcggcgtcat ttcagtgaac agcgccagcg acataccgtc gataccgcga
     6421 gtgatggtgg acattaaaat ccagatcagc cagaacaggc cgaaggccat cgtcgccatc
     6481 gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg cctgcatttt gcggcgagat
     6541 tcagccagcg ccgcagtggt ttgcatttca accatagcca ttagcgtgcc ccctcattct
     6601 tagccaggcg cataatcata aacttcgatg cggcgaggac gatgaaggta atcacaaaca
     6661 ggatcaggcc cagttccatc agtgcggcaa cgtgcagacc ggattccgct tccgcaaatt
     6721 cgttcgccag cgcagaggtg atactattgc ccggcatgta cagcgaggcg ctgtcgagct
     6781 ggtaggtgtt accgatgata aaggtcaccg ccatggtttc accgagcgcg cggcccagcc
     6841 ccagcatgat gccgccgata acaccatttt tggtgaacgg aagaacgata cgccagataa
     6901 cttcccaggt ggtgcagcca ataccgtagg ccgactcttt catcatcacc ggggtttgtt
     6961 cgaacacatc acgcattacc gccgcaatgt acggaataat catgatggcg aggatcacgc
     7021 ctgccgcgag gataccgata ccaaatgcgg ggccagagaa cagcgcgcca acaatcggga
     7081 tattcgacat gatattgccg accggctcct gaaagtaaac ggcgaacagc ggcgcaaaga
     7141 taaacaggcc ccacatgccg taaacgatac ttggaatggc tgccagcagc tcaatggcga
     7201 tacccagcgg gcgtttcagc cagccaggcg caagctcagt caggaacagg gcgataccga
     7261 aactcaccgg gacggcgatc agcagcgcga taaacgaagt caccaacgta ccgtagatcg
     7321 gcaccagcgc cccgtagata tcgttcggtg catcccactc tttggtccat aggaaagcca
     7381 gaccaaattt ctgaatgctc ggccaggagg agatgatcag agagacaata atgccaccca
     7441 acatcaatag cacaatcagc gccgccagtt ttaccagcac gctgaaaatt atgtcgccct
     7501 ttttacccgg tgggttaaaa gcgggcttgg ttgcagccat aagttactct tcagttaaac
     7561 gcgtttacaa agtcattttg taatgccgga tgcggcgtaa aacaccgtac ccggcctgga
     7621 gttttattag tacagcggct taccgctact gtctttaata ttggtcttcc acgcagcgcg
     7681 aacctgttca actacactat ccggcaggct ggcgtaatcc aggtcgttcg cctgtttagc
     7741 cccggttttg tacgcccagt cgaagaattt cagcacctct gtgccttgtt ctggtttctt
     7801 ctgatctttg tggatcagaa tgaacgtggt agaggtaata ggccatgcat cttcgccttt
     7861 ctggttggtc agatcctgag cgaaggtttt gctccagtct gcaccttttg ctgcattagc
     7921 gaagttttct tcggtcggac taaccggttt accatcagcg gagatcagtt tggtgtacgc
     7981 caggttgttc tgcttcgcgt aagcatattc aacataacca attgcacccg gcagacgctg
     8041 aacgaacgcg gcgataccgt cgttaccttt accgcccaga ccgatcggcc attttacggt
     8101 agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccaggt agctggtgaa
     8161 gacgaaggaa gtcccggagc catctgcgcg gcgtactaca gcaatgtttt gtgaaggcag
     8221 tttcagaccc ggattcagtt tggcgatggc ttcatcatcc cacttcttga ttttgcccag
     8281 gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca gccctggaat
     8341 gttaaccgcc agcaccacgc cgccaatcac ggtcgggaac tggaacagac cttcctgagc
     8401 cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa tcaacggtat tagcgataat
     8461 ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac cggtttcttt
     8521 ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac ctgcacctgt
     8581 caggcttgct tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg cgacaacagt
     8641 tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata aagcattgtt
     8701 tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatataa ggaatatgac
     8761 atttttatga cagaaaggaa ttaccaaata acttactgat tttaaatgtg aagatgaaaa
     8821 ttccacatgc catctttttg tcatgatgca tttaaaagtg ataaagaaat gggtgagatg
     8881 acaacgatgt gatgaacaat cgttgtcaga cctattagcg cacaatctca acagttatcg
     8941 tcgctgttgc agtaaattgc cccggggctg ggcgagctcc agtaaggctc gcaggagcag
     9001 ctgaaaaagt tacactacct gatttttcac catacactgt tgatttatcc atgtctgttg
     9061 gcaactcacc accgttcact ttgatttcct tattattttt gtcgtaaatt tttatcccca
     9121 cgttagagtt tgaagttacg accattgaat tgtccgtatt gggttcggta gcataaaaag
     9181 acgtactgat atcatcggta ttcagtatcc ccaggttttc acactcaata gtgactgttt
     9241 tagtaatttt atgtgttgtt accgcacttc caacaacaga tgaaaaatct gcagctggta
     9301 tttctttaaa atcaaaacaa actggcttta aattatttat ttcgcaactt tgcggagctg
     9361 taatatcgcc ccgaatataa acatcagcca tcggctgtga gtaatccacc ggggtgtcac
     9421 ggctaattgt tccatacaat gtcactatct ttgtttcagg aataatcaat tttcctgcaa
     9481 atgtcttctt gatataaaaa tatactatcg ctttagcccc actggatata cttaaaggtt
     9541 caatgcggtt gcaattgtac tttgtattac cagacgggtt gttagcatga tattcaaaag
     9601 gtgcattcac atacccaacc ccaagaatat ctatagcaat cccaacatca aggttcggat
     9661 taagatcata ataattaata ttagagtgaa ccccagcata agttaatgcc tggtttattt
     9721 ttgacgtata atagatatag gggaattctc ctacgttacc agtatc
//
LOCUS       DAJVPT010000126         9641 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVPT010000126 DAJVPT010000000
VERSION     DAJVPT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            17..190
                     /locus_tag="OQM47_003364"
     CDS             17..190
                     /locus_tag="OQM47_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1481395.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(239..1312)
                     /locus_tag="OQM47_003365"
     CDS             complement(239..1312)
                     /locus_tag="OQM47_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1481396.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1414..4125)
                     /gene="torS"
                     /locus_tag="OQM47_003366"
                     /pseudo
     CDS             complement(1414..4125)
                     /gene="torS"
                     /locus_tag="OQM47_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4208..5236
                     /gene="torT"
                     /locus_tag="OQM47_003367"
     CDS             4208..5236
                     /gene="torT"
                     /locus_tag="OQM47_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1481397.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5209..5901)
                     /gene="torR"
                     /locus_tag="OQM47_003368"
     CDS             complement(5209..5901)
                     /gene="torR"
                     /locus_tag="OQM47_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1481398.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            5988..7160
                     /gene="torC"
                     /locus_tag="OQM47_003369"
     CDS             5988..7160
                     /gene="torC"
                     /locus_tag="OQM47_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1481399.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7160..9641
                     /gene="torA"
                     /locus_tag="OQM47_003370"
                     /pseudo
     CDS             7160..9641
                     /gene="torA"
                     /locus_tag="OQM47_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
BASE COUNT         2323 a         2488 c         2589 g         2241 t
ORIGIN      
        1 ccagacgagg ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc
       61 cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga
      121 aattcgcttc atcgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa
      181 aataatgtga ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt
      241 agtgaccaat cattggcgct tgtggaatta agcgtcggta caattcctcc ggcaccgggc
      301 tttgccatac tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca
      361 gtagcgtcat taaccagcca gacaacgcaa aggctttttt atttgctgca ggtttttgca
      421 gtgaaaaggt caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac
      481 attccactgt tcgtacctga attaatttat ctaccgggat ccgtgatggg cacttttttg
      541 cgcatttgcc acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgacaata
      601 gcgaaaccac gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa
      661 taaacaggct ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac
      721 gaaagaaatc gagcatttta acgtccatca ccacgctata gggcgacaac ataaaatagt
      781 gaatcgcctg agcgggcatc aataacgcga tatagagaaa taaactcaac agcaaatact
      841 tcacgccgcg cagaggaata tccagccagc ggggaaggac acattgccga ccaaacagtt
      901 tgttaccgag atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgagcaaa
      961 aggccttttt gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg
     1021 cgtggacgga cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa
     1081 tcggtagcca gccttcgata cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt
     1141 cgtaatagcg cacccaatac cagaacgtga tggcaatata aatattcatt gccaatagta
     1201 agaattgcgt cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc
     1261 cgcccgtcgt gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc
     1321 cagtccgtta agttgcatac caaatgccac tattctagtt attcttaact ggctgatatt
     1381 gattcaaatc gcgttcaggt ctttcttatt caatcatgct tccagagcgg caacactgcg
     1441 tgtaatttct tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag
     1501 ctgactggcg ctacgcattc ccagactcga gcaactgctt tttagctgat gcgcggcacg
     1561 ctttattttc tcgctgtcct ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag
     1621 ggcatgttgt ttaaataatg ccagccattc gtggatcttc tccgtcccca ttaactgagc
     1681 gtcttcattg agttgcgaga catccagtgg tagatcgtta ttggcttgca gttggagata
     1741 gtgcgccagt aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa
     1801 tagtgaactg gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa
     1861 aaccagcgac ggatattgct gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg
     1921 cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc
     1981 ctgcgcggca ttgccaatag caacaacctg cgcaccactg gtgttcagca tctcaacggt
     2041 aattcgctgg gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt caagacgcac
     2101 cgcctgattg gctgtttttg gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca
     2161 aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc
     2221 cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc ttacctgcac
     2281 aaatggctgg aagatttctg ccagtttcgc gggatcaata ccgcagccgc tgtcttccac
     2341 ttcgaccagc cattgctcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcagt
     2401 aaaacgcagg gcattgctca ataggttggt aatgacctga cgaatacgtc gtggatcgcc
     2461 cattaacgcg ctcggtacat catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt
     2521 cacccgtccg ctcattaatt gcagggtact ttccagcagc gggcgcggtt caaagggctc
     2581 atcgctgact gaaacatttt tgccacctgc ttcgatagcc gaataatcga gaatatcgtt
     2641 gaggatggtc agcaacgatt cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc
     2701 gttaagtgcg gggttatctg ccagcagttg agcagtgccg agaataccgt acagcggtgt
     2761 gcggatctca tggctcatcg ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc
     2821 ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt
     2881 gacctgcgcc gccagctgtt cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc
     2941 atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga
     3001 gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt
     3061 gaatgagcga taaaccacgc gccagaggat cataatcagt gcgcaaagtg aaaccatccc
     3121 cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgtg ccagtccatg
     3181 ctgattgtgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa
     3241 ctgggcgata ttattttggg cgagagtttg caggtgattg ctgatttcac tgtcctgctg
     3301 gaacagcgcc aacagatcgc tatattggct aactgtagtt aacgttgttg cgacctgcgc
     3361 acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt
     3421 gagctgcttt tccagcgttg gtgcattttt ctggatctgc tccagcccca gattcatcac
     3481 catttgctgc acccgcagag cgctaaggcg cagttcattc atctggttaa catactcaag
     3541 atcgatatca atcagccgat cgagtgcact ttcagcagcc tgacgctgat cttgttcgat
     3601 caaatcgtaa atcccggcct gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc
     3661 ttgctccagg cgtgcgatct catcggcggc agctatctgc tgactgagtt gccgttgttg
     3721 ctggcgtagt tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat
     3781 ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag
     3841 caacgcatta atcttcaggc tttatgcggt gagcattcgc ccctgcgcct gccacatctt
     3901 ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct
     3961 caactggcgc gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg
     4021 gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat
     4081 cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa
     4141 ggtgaaaaag gttgagtcgc aaagtggaat gcatctagca taaagcctta ttattgatga
     4201 ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttgt cggcattttc
     4261 ggctgataac ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc
     4321 gcttaaagcc aaacgcgtat ggaaactgtg cgcgctttat cccagcctga aagattcata
     4381 ctggttatcg ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa
     4441 agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg
     4501 taaacagtgg ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgacct
     4561 gcaaaagcag gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccca
     4621 ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt
     4681 ggtgcaatgg gcgcacggta aaccactgaa cgtgctgctg atgcccggac ccgataacgc
     4741 cgggggcagt aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg
     4801 tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgcaacc tgttgcagga
     4861 gatgctggaa cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc
     4921 ggcaatgggt gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc
     4981 acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat
     5041 ggtctggcag ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt
     5101 gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgagaaca
     5161 tatccgccgc tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc
     5221 agcggctaag aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg
     5281 acgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc
     5341 agaaagcata cgcagcagac gctcacggct gagaatttcg cccggattag tcacaaatgc
     5401 caccaacatt tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt
     5461 atggcgcgac acattcaggc aataacccgc aaagcgatag cagtagtcct gagtgtgcgg
     5521 ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc
     5581 gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg
     5641 gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag
     5701 ggcgcgggtt aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc
     5761 taccggctga ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt
     5821 atacccctcc tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac
     5881 aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt
     5941 tgctcattaa gatcgcttca ctaaaccata attctacagg ggttattatg cggaaactct
     6001 ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc gcaattggga
     6061 ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc accagcacaa
     6121 ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat aaacagtcgg
     6181 tgcatttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg
     6241 atattacagg catggtgaag cgcaaactgg aagcgagcaa cgacatttac cagaccttta
     6301 ttgcccactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc
     6361 gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc cataactacg
     6421 atgcgatgga tcatgccagg cagcatcctg aagcggcacg tcagatgaag gtggcagcga
     6481 aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta ccggatatga
     6541 gtagcggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca
     6601 cgctgtactc tattgatatt aagccaattt atgcggcgaa aggcgataaa gaagcctctg
     6661 gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa
     6721 ttgaaattac cggctggacg gaaagcgccg gacgtcagcg tgtactcacc caattcccag
     6781 gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg
     6841 agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggcc accgcgtgga
     6901 tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcctatgcg gattcgttgt
     6961 acaacggcac ctgtaacgag tgccacggcg caccggaaat cgcccacttt gacgctaacg
     7021 gttggatcgg cacgctcaac ggcatgattg gctttaccag tctcgataaa cgtgaagaac
     7081 gcactttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag gctcacggcg
     7141 ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac gtcggcgttt
     7201 tctggcacaa ctcggcggct taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc
     7261 gcgccgtgca actgcggcgc aagcggcgac tgaggctgtc atctcgaaag agggcattct
     7321 taccgggtcg cactgggggg ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc
     7381 aaaaccgttc gaactggata aatatccgtc gaaaatgatc gccggattgc cggatcacgt
     7441 acacaacgcg gcacgtattc gttatccgat ggtacgcgtg gactggctgc gtaagcgcca
     7501 tctgagcgac acctcccagc gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc
     7561 cctcgacatg ttctatgaag aactggaacg cgtacagaaa actcacgggc cgagtgcctt
     7621 gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg ggatgctggc
     7681 gaaagctatt gccttgcatg gtaatagcgt tggtacgggc ggagattact ctaccggtgc
     7741 tgcgcaggtg atcctgccgc gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc
     7801 ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctccg atttgctgaa
     7861 aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt acgagcagct
     7921 aaaagcgaaa gtcgccgccg gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc
     7981 cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc cgcaaactga
     8041 cgtgccgctg caactggcgc tggcacatac gctgtacagt gaaaacctgt acgacaaaaa
     8101 cttcctcgct aactactgtg tgggttttga gcagttcctg ccgtatctgc tgggtgagaa
     8161 agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg accggcattg atgccgaaac
     8221 cattcgtggg ctggcgcggc agatggcggc gaacagaacg caaattattg ctggctggtg
     8281 cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtggttc tggcggcgat
     8341 gctggggcaa attggcctgc caggtggtgg ttttggctgg cactacaacg gcgcaggcac
     8401 gccggggcgt aaaggcgtta ttctgagtgg tttctccggc tctacgtcga ttccgcctgt
     8461 tcacgacaac agtgactaca aaggctacag cagcactatt ccgattgccc gttttatcga
     8521 tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc
     8581 gctgaaaatg tgtatttttg ccggaactaa cccattccat cgccatcagc agatcaaccg
     8641 cattattgaa ggctggcgca agctggaaac ggttatcgcc atagataacc agtggacctc
     8701 aacctgccgc tttgccgata tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct
     8761 cgaccagtac ggcaaccact ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc
     8821 gtagttcgag gcgcgcaacg actttgatat tttccgcgag ctgtgccgtc gctttaatcg
     8881 tgaagaagcc tttaccgaag ggctggacga aatgggctgg ctgaaacgca tctggcagga
     8941 aggtgtacaa caaggcaaag gacgcggcgt tcatctgcca gcgtttgatg acttctggaa
     9001 taacaaagag tacgtcgagt ttgaccatcc gcagatgttt gttcgccacc aggcattccg
     9061 cgaagatccg gatctcgaac cgctgggcac gccgagtggc ctgattgaga tctactcgaa
     9121 aaccatcgcc gatatgaact acgacgattg tcaggggcat ccgatgtggt ttgagaaaat
     9181 cgaacgctcc cacggtgggc ccggctcgca gaagtatccg ttgcatctgc aatctgtgca
     9241 tccggatttc cgacttcact cgcagctatg tgagtcggaa acgctgcgtc agcaatatac
     9301 ggtagcgggt aaagagccag tgttcattaa cccgcaggat gccagcgcgc gcggtattcg
     9361 taacggtgat gtggtacgcg tctttaacgc tcgcggtcag gtgttggcag gagcagtagt
     9421 ttctgaccgc tatgcacccg gcgtggcgcg aattcacgaa ggggcatggt acgatccaga
     9481 taaaggcggc gagcctggtg cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga
     9541 catcgggact tcgcagctgg cgcaggcgac cagtgcgcac actacgctgg tggaaattga
     9601 gaagtacaat ggaacagtgg agcaggtgac ggcgtttaac g
//
LOCUS       DAJVPT010000127         9632 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVPT010000127 DAJVPT010000000
VERSION     DAJVPT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OQM47_003371"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OQM47_003371"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1481400.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OQM47_003372"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OQM47_003372"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1481401.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OQM47_003373"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OQM47_003373"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1481402.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OQM47_003374"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OQM47_003374"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1481403.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OQM47_003375"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OQM47_003375"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1481404.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OQM47_003376"
     CDS             5060..6193
                     /locus_tag="OQM47_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1481405.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OQM47_003377"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OQM47_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1481406.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OQM47_003378"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OQM47_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1481407.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OQM47_003379"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OQM47_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1481408.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OQM47_003380"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OQM47_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1481409.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OQM47_003381"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OQM47_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1481410.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OQM47_003382"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OQM47_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1481411.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2447 a         2471 c         2458 g         2256 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgcgc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aaccactgag
     9541 aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc tgttgttact
     9601 ttttatatag ctaacaccaa taattaacct tt
//
LOCUS       DAJVPT010000128         9426 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVPT010000128 DAJVPT010000000
VERSION     DAJVPT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..421)
                     /locus_tag="OQM47_003383"
     CDS             complement(<1..421)
                     /locus_tag="OQM47_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1481412.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQ"
     gene            complement(613..2895)
                     /gene="pflB"
                     /locus_tag="OQM47_003384"
     CDS             complement(613..2895)
                     /gene="pflB"
                     /locus_tag="OQM47_003384"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1481413.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2950..3807)
                     /gene="focA"
                     /locus_tag="OQM47_003385"
     CDS             complement(2950..3807)
                     /gene="focA"
                     /locus_tag="OQM47_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1481414.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4213..5973)
                     /locus_tag="OQM47_003386"
     CDS             complement(4213..5973)
                     /locus_tag="OQM47_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1481415.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6103..6729
                     /locus_tag="OQM47_003387"
                     /pseudo
     CDS             6103..6729
                     /locus_tag="OQM47_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6928..8016
                     /gene="serC"
                     /locus_tag="OQM47_003388"
     CDS             6928..8016
                     /gene="serC"
                     /locus_tag="OQM47_003388"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1481416.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8087..9370
                     /gene="aroA"
                     /locus_tag="OQM47_003389"
     CDS             8087..9370
                     /gene="aroA"
                     /locus_tag="OQM47_003389"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1481417.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2393 a         2256 c         2373 g         2404 t
ORIGIN      
        1 tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca
       61 gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtatcc agacaggtat
      121 gaatgccttc ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg
      181 cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
      241 ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt
      301 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
      361 tacctgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
      421 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
      481 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
      541 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
      601 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
      661 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
      721 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
      781 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
      841 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
      901 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac
      961 agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
     1021 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
     1081 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
     1141 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcattgt taccaaactg
     1201 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
     1261 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
     1321 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
     1381 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca
     1441 gtccatgaag tgatccatgc gctccatcac ttcatcgtag ctcaggagat cgcctttgat
     1501 cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
     1561 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
     1621 gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat
     1681 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt
     1741 cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg
     1801 ccccatagtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
     1861 atcgaggccc ataccaccga tagattcggt tgcccagatt gggtcgccag agaacagttc
     1921 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
     1981 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
     2041 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
     2101 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
     2161 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
     2221 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
     2281 ctgtagagag gtgaactgag cgtatttgtc tttcatcagg tagtcgatac cgtacagcgc
     2341 aacgcgacgg tagtcaccga tgatacggcc acggccataa gcatctggca gaccggtcag
     2401 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
     2461 gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta
     2521 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
     2581 cggagcttca gtctgcagac caacaacttt ttccaacgct ttattgatgt agccagcgtc
     2641 gtgagaggtg atggtggaag caacagcggt gtcaaaatca actggcgcgt gagtgcggtt
     2701 ttccagttta acaccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
     2761 agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga agtcacggac
     2821 gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc
     2881 attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt
     2941 cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac
     3001 caacaaacca ccgccgataa tattaccgat cgtaaccggg atcaggttat cagtgatgaa
     3061 gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc
     3121 cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact
     3181 gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga acgctttgtc
     3241 catcaggctg cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag
     3301 gataccaaga cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag
     3361 gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc acattaaaag
     3421 tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttagccaa
     3481 ctgacaccag gtgatgcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa
     3541 gagatcggct ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac
     3601 cagttttgcc atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga tatagaagac
     3661 gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg
     3721 tttcgttgct ttatagacac ccgcctcttc ggccactttg gccattgcag caggaagtaa
     3781 aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga
     3841 tactaacaaa gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg
     3901 cccgtaactc gcatggtttt tatgcaaata cggagtaact atttgattat ccaaataaaa
     3961 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4021 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4081 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4141 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4201 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4261 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4321 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4381 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4441 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4501 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     4561 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     4621 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     4681 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     4741 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     4801 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     4861 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     4921 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     4981 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5041 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5101 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5161 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5221 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5281 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5341 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5401 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5461 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     5521 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     5581 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     5641 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     5701 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     5761 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     5821 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     5881 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     5941 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6001 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6061 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6121 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6181 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6241 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6301 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6361 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6421 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6481 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     6541 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     6601 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     6661 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     6721 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     6781 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     6841 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     6901 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     6961 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7021 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7081 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7141 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7201 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7261 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7321 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7381 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7441 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7501 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     7561 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     7621 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     7681 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     7741 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     7801 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     7861 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     7921 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     7981 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8041 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8101 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8161 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8221 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8281 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8341 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8401 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8461 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     8521 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     8581 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     8641 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     8701 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     8761 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     8821 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     8881 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     8941 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9001 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9061 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9121 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9181 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9241 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9301 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9361 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9421 acattg
//
LOCUS       DAJVPT010000129         9410 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVPT010000129 DAJVPT010000000
VERSION     DAJVPT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            71..1702
                     /gene="oppA"
                     /locus_tag="OQM47_003390"
     CDS             71..1702
                     /gene="oppA"
                     /locus_tag="OQM47_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1481418.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1788..2708
                     /gene="oppB"
                     /locus_tag="OQM47_003391"
     CDS             1788..2708
                     /gene="oppB"
                     /locus_tag="OQM47_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1481419.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2723..3631
                     /gene="oppC"
                     /locus_tag="OQM47_003392"
     CDS             2723..3631
                     /gene="oppC"
                     /locus_tag="OQM47_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1481420.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3643..4656
                     /gene="oppD"
                     /locus_tag="OQM47_003393"
     CDS             3643..4656
                     /gene="oppD"
                     /locus_tag="OQM47_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1481421.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4653..5657
                     /gene="oppF"
                     /locus_tag="OQM47_003394"
     CDS             4653..5657
                     /gene="oppF"
                     /locus_tag="OQM47_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1481422.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5710..6039)
                     /locus_tag="OQM47_003395"
     CDS             complement(5710..6039)
                     /locus_tag="OQM47_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1481423.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6074..7534)
                     /gene="cls"
                     /locus_tag="OQM47_003396"
     CDS             complement(6074..7534)
                     /gene="cls"
                     /locus_tag="OQM47_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1481424.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7677..7850
                     /locus_tag="OQM47_003397"
     CDS             7677..7850
                     /locus_tag="OQM47_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1481425.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7905..9158)
                     /gene="kch"
                     /locus_tag="OQM47_003398"
     CDS             complement(7905..9158)
                     /gene="kch"
                     /locus_tag="OQM47_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1481426.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2433 a         2223 c         2351 g         2403 t
ORIGIN      
        1 gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata atgagggagt
       61 ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg ttctggctgc
      121 gctaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca cactggcgga
      181 aaaacaaaca ctggtacgta acaatggttc agaagttcag tcattagatc cgcacaaaat
      241 tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac tggtcagcga
      301 tcttgacggt catccagcac ctggcgtcgc tgaatcctgg gataataaag acgcgaaagt
      361 ctggaccttc catttgcgta aagatgcgaa atggtctgat ggcacgccag tcacagcaca
      421 agactttgtg tatagctggc aacgttctgt tgatccgaac actgcttctc cgtatgccag
      481 ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga aaaaaccgat
      541 taccgatctc ggcgtgaaag ctattgatga tcacacatta gaagtcacct taagtgaacc
      601 cgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc caaaagccgc
      661 tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcgtcacca acggtgccta
      721 taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc cgacctactg
      781 gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt ctgaagttac
      841 cgatgtcaac cgctaccgta gtggtgaaat cgacatgacg tataacaaca tgccgatcga
      901 attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc catacctgtg
      961 cacttactat tacgaaatta acaaccagaa accgccattc aacgatgtgc gtgtgcgtac
     1021 cgcactgaaa ctaggtatgg accgcgatat cattgttaat aaagtgaaag cgcagggcga
     1081 catgcccgcc tatggttata ctccaccgta tactgatggc gcaaaattga ctcagccaga
     1141 atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc tggctgaagc
     1201 gggttatacc gcagacaaac cgttgaccat caacctgcgg tataacacct ccgatctgca
     1261 taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg taaacgtcaa
     1321 actggttaac caggagtgga aaacgttcct cgacacccgt caccagggca cttttgatgt
     1381 ggcccgtgca ggctggtgtg cagactacaa cgaaccaact tccttcctga acaccatgct
     1441 gtcgaacagc tcgatgaata ccgcgcatta taagagcccg gcctttgaca gcattatggc
     1501 ggaaacgctg aaagtgactg acgaggcgca gcgcacagct ctgtacacta aagcagaaca
     1561 acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg cgcgtctggt
     1621 gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct atacccggaa
     1681 tatgtacatt gtgaagcact aatggcaata cgtggggcag gagtgtcctg ctccacggtg
     1741 tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg ttaaaattta
     1801 ttctacgtcg ttgtctggaa gcgattccga cgctatttat tcttattact atttcgttct
     1861 ttatgatgcg cctcgcaccg ggaagccctt ttaccggcga acgtacttta ccgccagaag
     1921 tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca cagtatttca
     1981 gctacctgaa acaactggcg catggcgatt tcggtccatc gtttaaatat aaagattatt
     2041 cggtcaatga cctggtggca tccagttttc ccgtttctgc caaactggga gccgccgcat
     2101 ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgccgcatta aaacaaaaca
     2161 ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc cccagttttg
     2221 tggttgcgcc attattagtc atgatatttg cgatcatttt gcattggctg ccgggcggtg
     2281 gctggaatgg tggggcgctt aaattcatga tattgccgat ggtggcgttg tcactcgcct
     2341 atatcgccag tattgcgcgt attacccgtg gctctatgat tgaagtatta cactccaact
     2401 ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta cgccatgcat
     2461 taaaacctgc tctgttaccc gtgctctcct atatgggccc cgcatttgtc ggcattatta
     2521 ccggttctat ggtcatcgaa accatttatg gtttgccggg gattgggcaa ctgttcgtta
     2581 atggcgcatt gaaccgtgac tattccttag tgttaagcct gaccatcctg gttggtgcgt
     2641 taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac ccgaaaatcc
     2701 gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga cgctggaaaa
     2761 tttcagtgaa aagctggagg tcgaagggcg cagcttgtgg caggacgcac gtcgacgttt
     2821 tatgcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctgatcg cgttatttgt
     2881 aatcctggca ccgatgcttt cccagtttgc ctatgacgat actgactggg cgatgatgtc
     2941 cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg gtcgcgacct
     3001 gcttgtgcgc gttgcgattg gcgggcgtat ctcactcatg gtcggtgttg ctgcggcact
     3061 ggtggcagtg gtcgtgggga cactttatgg ttcgctttcc ggttatctgg gcggtaaagt
     3121 ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca tgttcttcgt
     3181 cattttgctg gtgacctttt tcggccaaaa catcctgctg attttcgtgg cgattggcat
     3241 ggtttcctgg ctggatatgg ctcgtattgt gcgtgggcaa accctgagtc tgaagcgcaa
     3301 agagtttatt gaggcggcac aagttggcgg tgtatcgacg ccgggcattg ttattcgcca
     3361 cattgtgccg aacgtactcg gtgtggtggt ggtctacgca tcgctactgg tgcccagcat
     3421 gatcctcttt gaatctttcc ttagcttcct ggggttgggt acgcaagagc cgttaagcag
     3481 ctggggggca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat ggttactgtt
     3541 gttcccagcg ggattccttg tggtgacact gttttgtttc aactttatcg gcgatggctt
     3601 gcgtgatgcc ctcgacccga aagatcgtta aggagtgcag ccatgagcgt aattgaaact
     3661 gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa agatttgcgt
     3721 gtcaccttta gtaccccgga cggcgacgtc acggcggtca atgatttaaa tttttcccta
     3781 cgtgccggag aaacgctggg cattgtaggt gagtctggtt cgggtaaatc gcaaactgca
     3841 tttgcgttga tgggcctgtt ggctgccaac ggacgcattg gcggatcggc aaccttcaat
     3901 gggcgtgaaa tcctcaattt gccagagcgt gaactcaata aactgcgcgc tgaacaaatc
     3961 tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt cggtgagcag
     4021 ttgatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt tgaagagtcg
     4081 gtgcggatgc tcgatgcggt aaaaatgccg gaagcacgta aacgcatgaa aatgtacccg
     4141 cacgaatttt ctggcggcat gcgtcagcga gtcatgattg cgatggcatt gctatgtcga
     4201 cctaagttgc tgattgcgga tgaacccact acagcgctgg acgtcaccgt acaggcgcag
     4261 atcatgacgc tattgaatga actgaagcgg gaatttaata ctgccatcat tatgatcacc
     4321 cacgatcttg gcgtggtggc ggggatctgt gacaaagtgc tggtaatgta cgccgggcgc
     4381 acgatggaat atggcaatgc gcgcgatgtc ttttatcaac ccgttcatcc ttattctatc
     4441 ggtttgctca acgcggtgcc gcgtcttgat gcggaaggtg aaacaatgtt gaccatcccg
     4501 ggtaatccgc caaacttgct gcgattaccg aaaggttgcc cgttccagcc acgttgtcca
     4561 catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc tggccgtctg
     4621 cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaactgaa ggaagaaaag
     4681 tcctccttga aatcgccgat cttaaagtgc actttgaaat caaagatggc aaacagtggt
     4741 tctggcaacc gccgaaaacg ctcaaagccg tcgatggtgt cactcttcgc ctgtatgaag
     4801 gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt gctcgcgcca
     4861 tcatcggttt ggtcaaggcg accgacggtc atgttgcctg gttaggtaaa gagttgctgg
     4921 gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt ttccaggatc
     4981 cgttggcatc gctaaacccg cgtatgacca tcggcgagat catcgctgaa ccactgcgta
     5041 cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg atgatgctga
     5101 aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctcc ggtgggcagt
     5161 gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt atctgcgatg
     5221 agccggtttc ggcgctggac gtgtcaattc aggcgcaggt ggttaacctg ctccagcagc
     5281 tgcaacgtga gatgggattg tcattaattt ttattgctca tgacctggcc gtggtaaaac
     5341 acatttccga tcgtgtgttg gtgatgtatc tcggccatgc agtagaactg gggacctatg
     5401 atgaggtcta ccacaatcca ctacatcctt acaccagggc attgatgtcg gcagtcccca
     5461 tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg gaattaccgt
     5521 cgccgatcaa cccgccttcc ggttgtgttt tccgtacccg ttgcccgatt gccggtccgg
     5581 agtgcgccaa aacacgtcct gttctggagg ggagtttcag acacgccgtt tcttgcctga
     5641 aagtcgatcc gctttaaaaa caataagggc tgacagttgt cagccctttt tcacgctaaa
     5701 agcgattatt tattcccgcc agatgatatg gcaaagtttg tgatcttttt cgcggcataa
     5761 cagaatgcgg gcgaaaacat cgctgatttc accatcttca ctgtccgcca gaccaatcac
     5821 cacttcggca aaaaagtccg ggttcaaatc gaaatcaaca tgttcctgcc agtcttccgc
     5881 cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca gcagaacatc
     5941 agctggatcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct gctcaagcgt
     6001 ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct gtttaacctc
     6061 tgttggcgac gttttacagc aacgggctga agaagtaaaa cagtcgctcg gcgacacgtt
     6121 gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc gaaatataat
     6181 cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc aaggtaatct
     6241 cgaaattcag ccacagacta cgcatatcaa ggttaacggt gccaaccaga cttagttcgc
     6301 catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa attttaaccc
     6361 cagcagcaag cagttccgta aagaatgcgc gactggccca gccgaccagc atcgagtcat
     6421 tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg caaatcgcat
     6481 gaagtaaatc atcgcttggc acaaagtagg gcgtggtcat gatcaaatat tcatgcgccg
     6541 aataagccgc agtcaataat gcctggtgaa tgagatcttc cggaaagccg gggccagaag
     6601 caattgtgtg aatggtgtga ccgctggcct gttcaaacgg cataatattg acatctggtg
     6661 gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa ataatcccca
     6721 tcgcggtggc gatggggcct tccatacgcg ccatcagatc aatccattgc cctacgcccg
     6781 catcttgttt gaagtagcga ggatcgacca tattcatgct gccggtgtac gcgatgtaat
     6841 tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa aacacacgca
     6901 tcagattgac ctttaaggct tcgaccactt caataccggc attacgcatt agctcgggcc
     6961 acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg caatgaatac
     7021 cacgccgtgc agccgccatt aatgattcag ccacctggtc cgccatgccg ccgggctgcc
     7081 agatataaaa caccatctca atattatggc gcgcgagctg gatgtcgcgg attaacgcct
     7141 gcatcacatc atctgactcg gtcatcagct gtagctgatt ccctttgacc ccagcgatcc
     7201 cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt tcttcggcga
     7261 agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc cacatcgctc
     7321 tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga taggcaataa
     7381 ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag ggaactgcgc
     7441 gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat cccagaatgg
     7501 ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt tgaaagcgca
     7561 ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga aaagcactgg
     7621 tcagcgtcgg gggcgggggt ataatgacca ttctgttatt gcatagagta gttaacatga
     7681 agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc cgacgtaaat
     7741 cgcgttggct tgaggggcaa tcgcgccgaa atatgcgtat ccacagcatc aggaagtgca
     7801 ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa atgtactggg
     7861 caccattgcg gtgcccaata agtttactct tccgtcttta aactctattt ttgcgccgat
     7921 tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatacc atgacctgag
     7981 ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa aactcgcgcc
     8041 agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac catcttaatc
     8101 ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact gctcatatct
     8161 tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc cagaatggcc
     8221 cggcaacgat cgattcccgc tttctttaat actgaactgt cattactgtc accggggata
     8281 acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc aggcaagttg
     8341 ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt gatggcgaga
     8401 atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt gtttcctttt
     8461 aaaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat agatgtggca
     8521 aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga ttcagaaaca
     8581 gggacaatat ctccatagcc gacggttgac atggtttcta tcgaaaaata gaacgcggtc
     8641 atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc gtaggttgag
     8701 taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc ggcgctactg
     8761 tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca aaatccaata
     8821 ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa caacaaaatg
     8881 atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt caggacaaga
     8941 aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa ctctgaaaat
     9001 gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt tttaaaaata
     9061 agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgtagggt cacccataaa
     9121 tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt aaccacaatt
     9181 ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgtgt gaatgtgtta
     9241 aacaatagta gatgacttta tccgcagtaa tcaaaggttt tgagtgagca actctgcgtc
     9301 aggaaagggc ttttttcctg aacatgctca catcatataa agaaatacag gtaaagttat
     9361 tatctgcttg tggtagtgaa tgcactgacc ggctataagg aaaggccaaa
//
LOCUS       DAJVPT010000130         9233 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVPT010000130 DAJVPT010000000
VERSION     DAJVPT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQM47_003399"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQM47_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1481427.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQM47_003400"
     CDS             1828..3147
                     /locus_tag="OQM47_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1481428.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OQM47_003401"
     CDS             3445..3486
                     /locus_tag="OQM47_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481429.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OQM47_003402"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OQM47_003402"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1481430.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OQM47_003403"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OQM47_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OQM47_003404"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OQM47_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OQM47_003405"
     CDS             5815..6711
                     /locus_tag="OQM47_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1481431.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OQM47_003406"
     CDS             6708..7682
                     /locus_tag="OQM47_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1481432.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OQM47_003407"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OQM47_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM47_003408"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM47_003408"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1481433.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2256 a         2255 c         2316 g         2406 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat ata
//
LOCUS       DAJVPT010000131         9212 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVPT010000131 DAJVPT010000000
VERSION     DAJVPT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            352..1470
                     /locus_tag="OQM47_003409"
     CDS             352..1470
                     /locus_tag="OQM47_003409"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1481434.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1536..1784
                     /locus_tag="OQM47_003410"
     CDS             1536..1784
                     /locus_tag="OQM47_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1481435.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1849..2217
                     /locus_tag="OQM47_003411"
     CDS             1849..2217
                     /locus_tag="OQM47_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1481436.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2311..2964
                     /gene="nfsB"
                     /locus_tag="OQM47_003412"
     CDS             2311..2964
                     /gene="nfsB"
                     /locus_tag="OQM47_003412"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1481437.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3072..4319
                     /gene="ybdG"
                     /locus_tag="OQM47_003413"
     CDS             3072..4319
                     /gene="ybdG"
                     /locus_tag="OQM47_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1481438.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4387..5763)
                     /gene="pheP"
                     /locus_tag="OQM47_003414"
     CDS             complement(4387..5763)
                     /gene="pheP"
                     /locus_tag="OQM47_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1481439.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5864..9007)
                     /gene="cusA"
                     /locus_tag="OQM47_003415"
                     /pseudo
     CDS             complement(5864..9007)
                     /gene="cusA"
                     /locus_tag="OQM47_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9019..>9212)
                     /locus_tag="OQM47_003416"
     CDS             complement(9019..>9212)
                     /locus_tag="OQM47_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="with CusA, CusC and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1481440.1"
                     /translation="DADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSE
                     ANISGALERMRSESATHAH"
BASE COUNT         2271 a         2558 c         2221 g         2162 t
ORIGIN      
        1 ttccagacct cagagtaaac ctgaaatcgg aaagcttcag gcacccgcag ttatcttacc
       61 cttcctcaac aaaatagctg tttatttata cagcaaatcc tgtttcggac ttctcactcc
      121 ccatttgatc aactgaagct gaacccgttt ttttctgcat gcctcaatcg tcaaaattct
      181 tcgccaccag ccagctctgc cataacttgc cacattgaaa aagtagccaa agtgcttcag
      241 gcaccatcag ttcaccatca ccaaaaaaaa gaaacattcc agaacttccg catctcgccc
      301 agccgctccg acttctatac tgaatagaaa acgtcaactt aagagaaacc tatgccatta
      361 cccgattttc atgtttctga accttttacc ctcggtattg aactggaaat gcaggtggtt
      421 aatccgccgg gctatgactt aagccaggac tcttcaatgc tgattgacgc ggttaaaaat
      481 aagatcacgg ccggagaggt aaagcacgat atcaccgaaa gtatgctgga gctggcgacg
      541 gatgtttgcc gtgatatcaa ccaggctgcc gggcagtttt cagcgatgca gaaagtcgta
      601 ttgcaggcag ccgcagacca tcatctggaa atttgcggcg gtggcacgcc cccgtttcag
      661 aaatggcagc gtcaggaggt atgcgacaac gaacgctatc aacgaacgct ggaaaacttt
      721 ggctatctca tccagcaggc gaccgttttt ggtcagcatg tccatgttgg ctgtgccagt
      781 ggcgatgacg ccatttattt gctgcacggc ttgtcacggt ttgtgccgca ctttatggcc
      841 ctttccgccg cgtcgccata tatgcaggga acggatacgc gttttgcccc ctcacgaccg
      901 aatatttttt ccgcctttcc tgataatggc ccgatgccgt gggtcagtaa ctggcaacaa
      961 tttgaagccc tgtttcgctg tctgagttac accacgatga tcgacagcat taaagatctg
     1021 cactgggata ttcgccccag tcctcatttt ggcacggtgg aggttcgggt gatggatacc
     1081 ccgttaaccc ttagccacgc ggtaaatatg gcgggattaa ttcaggccac cgcccactgg
     1141 ttactgacgg aacgcccgtt caaacatcag gagaaagatt acctgctgta taaattcaac
     1201 cgtttccagg cctgtcgcta tggtctggaa ggcgtcatta ccgatccgca cactggcgat
     1261 cgtcgaccgc taacggaaga taccttgcga ttgctggaaa aaatcgcccc ttcagcacat
     1321 aaaattggcg catcgagcgc aattgaagca ctgcatcgcc aggtcgtcag cggtctgaat
     1381 gaagcgcagc tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg gctggtgaaa
     1441 aagcattgtg agatctgggc cggtgactaa gccgggaatt gccttacaga agaatatccc
     1501 tgacaatggt gttttaactt acatttaaca atgttatgaa acacccttta gaaaccttga
     1561 ccaccgcagc aggcattttg ctgatggctt tcctctcttg cctgctgctg cccgcccccg
     1621 cactggggct gacgctggca caaaaactgg tgaccatgtt ccatctgatg gatcttagcc
     1681 agctttacac tttattgttt tgtctgtggt ttttagtgct gggcgctatt gagtattttg
     1741 ttctgcgctt tatctggcga cgctggttct cgctggcgga ttaaacgtcg attatcgccg
     1801 aacttcgcgg catactttgc ttattctcct tcgcctaaag gaacgtttat ggataagcaa
     1861 tcactgcacg aaacggcgaa acgcctagct cttgagttac cctttgtcga gctttgctgg
     1921 ccttttggcc cggagttcga tgtttttaaa attggcggca agatttttat gctgtcgtcg
     1981 gagctacgcg gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc cctgttaaat
     2041 cagcaaatat acccaagcat taagccaggg tatcacatga ataaaaagca ctggatttcg
     2101 gtgtattccg gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg
     2161 aatctggtgg ttgatggtct ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc
     2221 gggaatctat agcgcatttt tctcgcttac catttctcgt tgaaccttgt aatctgctgg
     2281 cacgcaaaat tacttacaca tggagtcttt atggatatca tttctgtcgc cttaaagcgt
     2341 cattccacta aggcatttga tgccagcaaa aaacttaccc cggaacaggc cgagcagatc
     2401 aaaacgctac tgcaatacag cccatccagc accaactccc agccgtggca ttttattgtt
     2461 gccagcacgg aagaaggtaa agcgcgtgtt gccaaatccg ctgccggtaa ttatgtgttc
     2521 aacaaacgta aaatacttga tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg
     2581 gacgatgcct ggctgaagct ggttgttgac caggaagatg ccgatggccg ctttgccacg
     2641 ccggaagcga aagccgcgaa cgataaaggt cgcaagttct tcgcctatat gcaccgtaaa
     2701 gatctgcatg atgatgcaga gtggatggca aaacaggttt atctcaacgt cggtaacttc
     2761 ctgctcggcg tggcggcgct gggtctggac gcggtgccca tcgaaggttt tgacgccgca
     2821 atcctcgatg cagaatttgg tctgaaagag aaaggctaca ccagtctggt ggttgttccg
     2881 gtaggtcatc acagcgttga agattttaac gctacgctgc cgaaatctcg tctgccgcaa
     2941 aacaccacct taaccgaagt gtaattctct cttgccgggc atctgcccgg ctatttcccc
     3001 tcagattctc ctgatttgca taaccctgtt tcagccgtca tcataggctg ctgttgtata
     3061 aaggagacgt tatgcaggat ttaatatccc aggttgaaga tttagcgggt attgagatcg
     3121 atcacaccac ctcgatggtg atgattttcg gtattatttt tctgaccgcc gtcgtggtgc
     3181 atattatttt gcattgggtg gtactgcgga ccttcgaaaa acgtgccatc gccagttcac
     3241 ggctttggtt gcaaatcatt acccagaata aactcttcca ccgtttagct tttaccctgc
     3301 aggggattat cgtcaatatt caggcggtat tctggctgca aaaaggcacc gaagcggcag
     3361 atattctgac tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt tcagtcttct
     3421 cgttgctgga tgttattttg aatctggcgc agaaattccc ggcggcatct cagttaccgc
     3481 tgaaagggat atttcagggg attaaactga tcggcgcaat cctggtcggc attttgatga
     3541 tctcgctgct gattggtcag tcgccagcga ttctaatcag cggtcttggt gcaatggctg
     3601 ccgtgctgat gttggtattt aaagatccga ttcttggtct ggtggcaggt attcagcttt
     3661 ccgcgaacga tatgctgaaa ctgggcgact ggctggaaat gccgaaatac ggcgcggatg
     3721 gcgcggtgat cgatattggg ttaaccaccg tcaaagtgcg taactgggac aataccatta
     3781 ccactattcc cacctggtct ctggtttctg actcctttaa aaactggagc gggatgtcag
     3841 catctggcgg gcgacgtatt aagcgcagta tcagtattga tgtcaccagt attcgttttc
     3901 ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt gttaaagcct tatttaacca
     3961 gccgccatca ggaaattaat gagtggaatc gccagcaagg ttctacggag tcggtattaa
     4021 acctgcgccg aatgaccaat attggaacct ttcgtgccta tctgaacgaa tatctgcgta
     4081 accatccgcg gattcgtaaa gatatgacct taatggtacg ccaactggct ccaggtgata
     4141 acggtttacc gctcgagatc tacgcgttta ctaacaccgt ggtgtggctg gaatatgaaa
     4201 gcattcaggc tgatatattc gatcacatat ttgcgattgt cgaagagttt ggtctgcgac
     4261 ttcatcagtc gccaaccggc aatgatattc gctctctggc gggtgcattt aagcagtaat
     4321 taaaaaaacc gctctcatcg aatggatgag agcggacatc gttttgtcgg atgtagcgtg
     4381 aatgccttat ttccgacgca gcgttttaaa tgccacaaac aggaatacaa tccacaccgg
     4441 cagcaggatc gctgacaagc gcatatcatc catcgtgcac atcagcagca aaatcatgcc
     4501 gaggaaggcg atgcaaagat agttgccgaa cggatagagc agcgccttaa actgtgtttc
     4561 acgcccctga cgtcgcatcg ccgcgcgaaa acgcagatgc gccaggcaga tcataatcca
     4621 gttcaacagc aacgttgcta ccaccagcgc catcagcaga ccaaacgctt tttgcggcag
     4681 cagatagttg attaacacca ccagcgaagt gatcgctccg gaaagcatca gcgagttaat
     4741 cggcacaccg cgacggctga cgcgagtcaa aaacttcggc gcattaccct gcacagaaag
     4801 gccaaacagc atgcggctgt tagagtaaac cccgctgtta tacaccgaca gcgatgccac
     4861 tagaatgacg aagttcagcg cagaagctac cacattgctg tcgagattat ggaaaatcat
     4921 cacaaacggg ctactgttgg atttcacttc cacccacgga tagagtgcca gtaaaaccac
     4981 cagtgaaccg atataaaaca gcaggatgcg atacaccacc tgatttaccg cttttggaat
     5041 gcttttttcc ggatcgcgcg cttcagcggc tgtaatccca atcagctcca gaccgccgaa
     5101 ggagaacata attaccgcca gcgacaaaat cagcccattc cagccggtgg cgaagaaacc
     5161 accgtagcgc cagaggttgt cgatactggc tttctcgccg ccgtaaccag aaaacagcag
     5221 ccacaggcca aagccgatca taccgatgat cgccagcact ttaatcagcg caaaccagaa
     5281 ctcggtttcg ccatataagc gcacgttcac caggttaacg gcgttgatga taataaagaa
     5341 ggcggcagcc caaatccacg ttggaacatc cgggaaccag tactgcatat agatgcccgc
     5401 agcggtcagc tctgccattc ccaccagcac gaacattacc cagtagttcc agccagagag
     5461 gaagcccgca aacggtcccc agtatttata ggcaaagtgg gcaaatgaac cggataccgg
     5521 ctcttcgacc accatctcgc caagctggcg cataatcagg aaagcgatga tcccggcgac
     5581 gccgtagccc agcaatacag ccggacccgc catctgaatc gccgggccaa tgccaagaaa
     5641 cagaccagta ccaattgcgc cacccagcgc aatcagttga atatgacggt tatgtaatcc
     5701 gcgatgaagc gtcggctctt gattcgacgc agtatcttcc gatacggttg acgcgttttt
     5761 cacgcctttc ccctgtgtgt cttttttgtt gaggggcacc ttttaacatt tagtgcccat
     5821 cgtcgcaaga cacaatccac acggttaaac gaggtatcct gctttaattc cgtacccgat
     5881 gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggtg
     5941 cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct gaaccagccc
     6001 ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc atcgctttcg
     6061 ggcgcacgcg caggaccgcg ccgtgataca acgcctcatc cagcttctgc tcgctgaatg
     6121 tttgtggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca
     6181 ccacgccaaa ttcggcggcg accccggcaa gggcaataaa gccggtaccc gtcgccacag
     6241 aaagatgaaa gcccatccac cacagtaacc agatgccgcc caccagcgca aacggtacgc
     6301 tgctgataat cagcaacgct tcgcccaccc gacggaatgc cagatacaac agcacgaaga
     6361 tgatcatcag tgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct
     6421 cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg
     6481 ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc
     6541 agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggtagag actttaacgt
     6601 cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggc aactggcgca
     6661 gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga taacgggcaa
     6721 tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca
     6781 catccgccac cgtcataccg taacgtgcgg ctttttcacg gttaatctca acgttgatat
     6841 agcgcccacc ttccagccgc tcagcaagcg cagaagctac gcctggcacc gttcgcgcca
     6901 cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa
     6961 tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca
     7021 cccacagatt cgccagcccc ggcagacgca cggtgttatc cagttcctca atgattttgt
     7081 ccattgtcat gcctggccgc cactgatcct gtggcttaag ctggatggtc gtttctacca
     7141 tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg
     7201 ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg
     7261 ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg
     7321 gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgccg
     7381 ccaccagcag cgtggttttc ggccagtgca gtactttcag caacagcgga tgataaacac
     7441 gaatcaaaaa gcgattgagc ggattactgc tttccggcgg aattttgcca cggatccagt
     7501 aacccatcag gatagggatc actacgatcg ccagcagcgc cgcacccgcc atcgcatacg
     7561 ttttggtgaa cgccaacggg ccaaacagac gcccttcctg cccttccagg gtgaagatcg
     7621 ggataaacga caacgtgata atcagcagac tgataaacag cgccggcccc acttcaacag
     7681 acgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt
     7741 gctgccactc ttccagccgt ttatgcgcat tctcgatcat gacgatagcg gcatcgacca
     7801 tcgccccgac ggcaatcgca atgccaccca gcgacataat attggcattc agcccctgga
     7861 agggcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg
     7921 ccgagcgcac atgccagaga aacggcgcgc agactaccgc caccacaata aactcttcca
     7981 gcaacttgcc gctgaggttg tcgatggccc ggtcaatgag ctggctgcgg tcgtatgttg
     8041 taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg
     8101 cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg cccgccactt
     8161 cgccttcgcc gtttagttcg gcaatgcccc ggcgcatctt cgggccaatc tggaccttcg
     8221 caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa
     8281 agtcgtcgag cgtttgtaga tagccgctgg cgcgcaccat atattctgct tccgccagtt
     8341 cgattgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcga
     8401 gactgatgcc atactgcgcc aggcgctgag gatcgataac cacctgatac tctttcacca
     8461 caccgcctac cgacgccact tccgcaacgt caggaatggt tttcagctca tatttgagaa
     8521 accagtcctg caatgagcgt aaatcggcaa gatcgtgctt accgctgcga tccaccagtg
     8581 catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg ctgactcccg
     8641 caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg
     8701 gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgc gagaaaccac
     8761 gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat
     8821 tttcaacgat ttgcggtgcc tggccgggat agctggtttt aataatcacc tgcacatcgg
     8881 agagatccgg caacgcatcc actggcgtat taatgatggt ccaggtgccc tagatgctca
     8941 gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt
     9001 caatcattgg ttattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag
     9061 tgcgccagaa atattggctt cggaatcaat caggaacagg ccgccggaaa ccaccttttc
     9121 accttccgcc agaccagagc gtaatgcggt gacgccttgc gatgcctgga aaacagcaac
     9181 gcgtttcggt acaaagcgcc cgtcggcatc aa
//
LOCUS       DAJVPT010000132         9164 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVPT010000132 DAJVPT010000000
VERSION     DAJVPT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..742
                     /gene="hchA"
                     /locus_tag="OQM47_003417"
     CDS             <1..742
                     /gene="hchA"
                     /locus_tag="OQM47_003417"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1481441.1"
                     /translation="GVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLP
                     LYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVAS
                     LNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHS
                     DNPLNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDR
                     KRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(850..2208)
                     /gene="hprS"
                     /locus_tag="OQM47_003418"
     CDS             complement(850..2208)
                     /gene="hprS"
                     /locus_tag="OQM47_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1481442.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLTVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2208..2879)
                     /gene="hprR"
                     /locus_tag="OQM47_003419"
     CDS             complement(2208..2879)
                     /gene="hprR"
                     /locus_tag="OQM47_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1481443.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3012..3425
                     /gene="hiuH"
                     /locus_tag="OQM47_003420"
     CDS             3012..3425
                     /gene="hiuH"
                     /locus_tag="OQM47_003420"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1481444.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3534..4538
                     /gene="msrP"
                     /locus_tag="OQM47_003421"
                     /pseudo
     CDS             3534..4538
                     /gene="msrP"
                     /locus_tag="OQM47_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4539..5174
                     /gene="msrQ"
                     /locus_tag="OQM47_003422"
     CDS             4539..5174
                     /gene="msrQ"
                     /locus_tag="OQM47_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1481445.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5432..6082
                     /gene="zinT"
                     /locus_tag="OQM47_003423"
     CDS             5432..6082
                     /gene="zinT"
                     /locus_tag="OQM47_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1481446.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7160..7429)
                     /locus_tag="OQM47_003424"
     CDS             complement(7160..7429)
                     /locus_tag="OQM47_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1481447.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7486..8154
                     /locus_tag="OQM47_003425"
     CDS             7486..8154
                     /locus_tag="OQM47_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1481448.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8470..>9164
                     /locus_tag="OQM47_003426"
     CDS             8470..>9164
                     /locus_tag="OQM47_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1481449.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2626 a         2062 c         1876 g         2600 t
ORIGIN      
        1 tggcgtggac tatccaaaac cgtatcgcgg taaacataaa attctggtga tcgccgcgga
       61 cgaacgttat ttgccgaccg ataacggaaa actgttctcg accggtaacc atccgattga
      121 aacgttgctg ccgttgtatc atctccatgc tgcaggtttc gaattcgaag tggcgaccat
      181 ttccggtctg atgaccaagt ttgaatactg ggctatgccg cacaaagatg aaaaagtaat
      241 gccattcttt gaacagcata aatcgttatt ccgtaatccg aagaaactcg cggatgttgt
      301 cgccagcctc aacgctgata gcgaatatgc agcaatcttt gttcctggtg ggcatggcgc
      361 acttattggt ttaccagaaa gccaggacgt ggctgccgct ttacagtggg caatcaaaaa
      421 tgaccgtttt gttatctccc tttgccacgg tccggcggct tttctggcac ttcgccacag
      481 cgataaccca ctgaatggtt attctatttg cgcattccca gacgccgcag acaaacaaac
      541 gccagatatt ggctatatgc cgggtcatct cacctggtac ttcggcgaag agttgaagaa
      601 aatgggcatg aacatcatta atgacgacat caccgggcga gtacataagg accgtaaacg
      661 tctcaccggc gacagtcctt ttgcagcgaa tgcgttgggt aaactggcgg cgcaggaaat
      721 gctggcagct tacgcgagtt aatccctgtt acattgtgat gtaagcgcat aatcaatcgc
      781 agccacgtta atactcacga cgtggctgcg ttttttcacg gttaacttat ggcgtactga
      841 agcccgatgt taatttcttt gcggtaacgt aattcggaac acgttatgct tactgagata
      901 gtgatacgta gcacttcctc catgtaattc ggcaatcgct ttgactaaag aaaggccaag
      961 tccctgacct acggaatgac gcgaattatc tccccgccaa aatctacgga agagtttttc
     1021 aggctcatta attttcgttc caggactggc gatatcaata ttaagagagc cgttggcatc
     1081 aagaaaactg gttatatgaa tacgcgattt ttctggtgaa tatcgaatgg cgttaacagt
     1141 aagattcgat aacattcgtt gcagtaaaat tttatccgca aagatttgtt gattgcactt
     1201 gaccttaaag caaatctctt tctcgtctga aagatattca agatagtcca aaagattttc
     1261 aacttcctta ttgagagaaa gcgaatccag tttcaccaaa acattgtttt tgtctgccct
     1321 ggcaagaaac agtatattct ctgttaaccg cgaaatattt tccagctctt caatgtttcc
     1381 ggcaattgtt ttttgatatt cagcgatact tctgatttga ctgagcgtaa cctgattctg
     1441 acccagtaat gcattaattg gcgttctaag ttcatgagcg agatcgtcag caaattgact
     1501 aaggcgctca aaatctttga ctaaagcatg atgcatttta ttcaacgcct gcccaagagg
     1561 ttttagttct ctcggtaatg cgttaacctc aaccggcact cggctatcgt tataattcag
     1621 cgcttccgtt acgccactca actttttgat ctctcgcaac cccgtcctga ttaacaacgg
     1681 acttaatact gagcacagta taatggcgac aatgcaaatt ataatgctat taattttata
     1741 ctgttcaagc atattatgtc tggctgaagc caatttagcc acagtaaccg ttaatgatgg
     1801 cgaaacttca tcaatgttaa ttcgtaaagc atctatctct gtatcattaa taatgcttct
     1861 gtaaatgcct gctgcgctga ttgtctcact agcaggtatg ttatttaaca tgtcatcact
     1921 gacatttgtc cggttaacaa ttttattgat gccatcacta tgaatgatca aaatatcctg
     1981 actaacatcc atcatccggt taaagtacac aggtaacgta tctggattta ccccatcaat
     2041 taacaactgc ttgatctgcg ctgtccggtt aatgagtgtt gtatcatcgc gccatttcaa
     2101 ctcacttgcc aggccattat agagagtcca tacaattcca gcgccagcaa cagactgcag
     2161 aaatataaaa agcaaggtta aacggacggt tatagataat cttttcatta tttttttacc
     2221 gctacgaatg aatagcccat cccccggatt gtggcgatta gcttttcagg aaaaggatca
     2281 tcaacttttg cgcggagcct gcgaatggcg acatccaccg tattggtatc actatcaaag
     2341 ttgattcccc aaatttcact cgcaataacc gttctgggta taatctcgcc agctctggag
     2401 gccagtagcc aaagtaactg aaactccttg cgcgtcagtg taatactgat attgtccctg
     2461 ctgacacttt gactgacaga gtccattttt aagccactga tttctaatgt tgaattcaga
     2521 gcgtgatgtt gccttaattg tgcccgaacc cttgctagca actcagaaaa tgaaaaaggt
     2581 tttaccagat aatcatttgc cccactgtcc aaccctctga ctctgtcatc gacagaatcc
     2641 cttgcagtaa ggcaaataac aggggtttgc tttgccgttc ttaacgtttg taagatctgc
     2701 cagccatcca tacccggaag cataatatcc agaatgatca atgcataatc atccttcagc
     2761 gcaagataaa gcccatctct gccatcagaa acggcatcaa tgacataacc cgcttcggaa
     2821 agcccctgcg ttacccattc ctgggtcctt tgattatctt caataagtag aatcttcatg
     2881 aaaataatat gccataaatc atatgttata gaaacaacct ggttcattac aaaattgtaa
     2941 tgctgctgta aggttaccct ggacgttttt tcgctatcct caaaactcat tcacatgaca
     3001 aggatataaa catgttaaag cgttatttag tactctcggt agcaacggca gccttttcat
     3061 taccttcttt ggtttatgcc gcacaacaaa acattcttag cgtgcacatt ttgaaccagc
     3121 aaacaggaaa acccgctgcc gacgtgacag tcactcttga aaagaaagcg gataacggct
     3181 ggttacaact taataccgcc aaaacagata aggatggacg aattaaggca ctgtggcccg
     3241 agcaaactgc gactactggc gattaccgtg tcgtatttaa aaccggggac tatttcaaga
     3301 aacaaaatct tgaaagtttc ttccctgaga ttcccgttga atttcatatt aataaagtga
     3361 acgagcatta tcatgtgcct ttgttactca gccaatatgg gtattcaacc tatcgtggca
     3421 gttaatgcgt aaatatgtaa aagctgatct ctgctactgt tagggaggtg actccctgac
     3481 agattaacag taaacagctc ttgttggtta acgacaaaaa agtgtgatgg cttatgaaaa
     3541 agaagcaatt tttaaaagaa tcagatgtta cggccgagtc ggtattcttt atgaagcgtc
     3601 ggcaggtgtt aaaagcactg ggcatcagcg cagctgcatt ttctttgcct cacgctgcgc
     3661 atgccgatct gcttagctgg tttaaaggga acgatcgtcc gcccgccccc gccggaaaac
     3721 cgctggagtt cagcaagcct gccgcctagc aaaataacct gccactgacg ccagcagata
     3781 aagtctccgg ttataacaac ttctatgaat tcgggctgga taaagacgat cccgcggcta
     3841 atgctggtag cctgaaaacc gatccatgga cactgaaaat cagcggcgaa gtggcaaaac
     3901 cattgaccct cgatcatgat gatttaaccc gtcgcttccc gctggaagag cgtatttatc
     3961 gtatgcgctg cgtggaagcg tggtcgatgg tggtgccgtg gattggtttt ccgctacaca
     4021 aattgctggc gcttgccgaa cccaccagca atgcgaagta tgtcgctttc gaaacgattt
     4081 atgcgccgga acagatgcct ggccagcagg accgctttat cggcggcggg ctgaaatatc
     4141 cttatgtcga aggattgcgt ctcgacgaag caatgcatcc gctcacactg atgaccgtag
     4201 gtgtttatgg caaggcgtta ccaccgcaaa atggcgcgcc ggtgcgactg attgtgccgt
     4261 ggaaatatgg ctttaaaggg attaaatcga tcgtcagtat taagctgacc cgcgagcgtc
     4321 cgccaaccac ctggaatctg gcagcgcctg acgaatacgg tttttacgcc aacgttaatc
     4381 cgcatgttga tcacccgcgc tggtcacagg ctaccgaacg atttattggt tcaggcggca
     4441 ttctcgatgt tcagcgccag ccaacactac tgtttaatgg ttacgccgac caggtggcat
     4501 cgctgtatcg tggcctggat ttgcgggaga atttctaaat gcgtctgaca gcaaaacaag
     4561 tgatatggct gaaagtttgc ctgcatcttg ccggattgtt gccgtttctc tggctggtct
     4621 gggcgatcaa tcacggtgga ctgggtgccg atccggtgaa agatattcag cattttaccg
     4681 gtcgcactgc actgaaattt ttgcttgcgg ccttgttaat cacacctctg gcacgctacg
     4741 caaaacagcc gttattgata cgcactcgcc gcctgttagg cttatggtgc tttgcctggg
     4801 cgacactgca tttaaccagt tacgcattgc tggagttggg cgtgaataat ctggcgttat
     4861 taggtaagga gttaattacc cggccttatt taacgttagg aattatcagc tgggttattt
     4921 tgcttgcttt agcgttcact tcaacccagt cgatgcagcg aaaactcggc aaacactggc
     4981 aacagttgca taacttcgtc tatcttgtcg cgatcctggc cccaatacat tatctgtggt
     5041 cagtgaagat tatttcaccg cagccactca tctacgccgg gctggctgta ctgcttttag
     5101 ccttacggta taagaagttg ctttcccttt tcaaccggtt acgcaagcag gcccacaaca
     5161 aattatctct ataacttcat attgccgaca aacttcgcgt tatcccggcc ggatgcggcg
     5221 cgagcgccct atccggccca cacagatcgc atcaagttat gtttcgtttg tcatcaacct
     5281 ttcatcacca gaataattta ttttatttca ataagatagt tcaatacacc tgagaatacc
     5341 atgctctcat ttcctaatga gttgttgcat tttttcatat gttacaatat aacattacac
     5401 ataatgtaca ttaactttgg aggaaattgt tttggcgatt cgtcttcata aactggctgt
     5461 tgctttaggt gtctttattg ttagcgctcc tgccttttcg catggtcatc attctcatgg
     5521 caaaccctta acagaggtcg aacaaaaagc tgctaatggt gtttttgatg ataccaatgt
     5581 acaaaaccga acgctcagtg actgggatgg agtctggcaa tccgtttacc ctttactgca
     5641 aagtggcaaa cttgatcccg tctttcagaa gaaagcggat gcagataaaa ccaaaacatt
     5701 tgctgaaatt aaagattatt atcgcaaagg ttatccgaca gatatcgaaa tgattggcat
     5761 tgaggacggc attgtcgaat tccatagaaa taatgaaaca acatcctgta aatatgatta
     5821 cgatggatac aaaatactca cctataaatc aggcaagaaa ggcgttcgtt acttattcga
     5881 atgtaaagat cctgaaagca aagcccctaa atatatacaa tttagcgatc atataattgc
     5941 accacgaaaa tcatctcatt ttcacatttt tatgggtaat gattcccagc aatctttatt
     6001 gaatgaaatg gaaaactggc caacgtatta tccctatcag ttgagtagcg aagaagtggt
     6061 cgaggagatg atgtctcatt gatttttttg cgctgacttc agttatttaa cagaacagtg
     6121 cctggtagta acaacaataa tttaccaaga caaataagta agacaaactc agacacgctc
     6181 atacttgagt cgtgtctttt ttattatgtc tgtagaaaaa aactgagaaa aaggctaaca
     6241 ataaatacac aactcagcca tacagactgg cagcgcaaat gtttccagga gcaagtcatc
     6301 tgtcatggta aagctaacca gaaatatagg ttaattttgg tgtttaaacc ttttttactt
     6361 gttagtatga tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaaattat
     6421 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttggctc aattattgac
     6481 cccttctcta cgcaacttca gttcccgcca gcaactttgc ggcagtttca taggatcaat
     6541 gtctaaaaga ataatggtga ccgataagaa aacgactgaa taactgtaga ttttcgctcg
     6601 aaaccttcct gtcagctcca tagcgaatca agcgctgaat gtcacagtat cgaacagaaa
     6661 acagtgacga tctaaccctt caagaatatt ctacgattgt tctgtttagg aaaagcaagg
     6721 cgggaagtcg ggagataagt cattgataaa atggcggaga gagggggatt tgaacccccg
     6781 gtagagttgc ccctactccg gttttcgaga ccgatgatac agagtcgaaa aatcaaagag
     6841 ttaaaaattt ttcttggaaa aaatgtctta aaatcgtaca cgaaaaatca atgaattaca
     6901 taacattaaa gaagaatatt ccaagccttc ttgagtccac cacctgtttt caggtgtcat
     6961 tcagacgtga tttgccccag attgggaaag cacgcttttt tctcagctta ttccgttttg
     7021 cctgtgtgtt gaataattcc actgcagtta tataaaatcg gtaacggctg gaaatcattc
     7081 aatactcgca ctatcgaaag tttaccagcc aaccgcagca cgtcctgcat acgtcgtgtc
     7141 tgcggttttt ctttttcgct tacattgtgt ctggttcttc cggccactca atatcaggtg
     7201 cagttgatgt atcaacacgg ttcagcaaca cccgatactt cttccaggct tccagcaacg
     7261 agttttcttc ctccgttgcg atctccagat ctacagcatc ctgaagtggc gcaatatgct
     7321 cactgaatcc ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct
     7381 gtatcgaagc ataccaggct atttcaatat cgctatgctg cggcagcatt taaccccttg
     7441 taattcatcg ccataattga tttaattcac aaataaaact ataatatggt gaaattaatg
     7501 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
     7561 tggtgggttt tgggagtatc aaatcggctt gcatggggat gtcctacaaa ggaacacctt
     7621 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
     7681 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
     7741 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
     7801 gatgtttttg aaccttatcc tgcggcgtta catggtcaat ttgattccat ttccatgttt
     7861 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
     7921 gcgcaggcct taactgatga tggaactcta tacggagcca caattcttgg cgatggagtt
     7981 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
     8041 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
     8101 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
     8161 accgcagcac gttcttgctt aagacgtgct acggtttcct ttacgatttg gggttgcgac
     8221 tttacctcta ttgataatgc attccggcag aacgttcaaa tatgaacgta cgatatttaa
     8281 ctaaccgaaa aacaaaatat agcacaggcg agacatttac catctaagaa aatagtatcg
     8341 tttttttaca gttaaatcaa catcacttcc ttaaaatgaa aacaataaat ttcaatcctg
     8401 aaacatttta tttcagctta ttattcccca gtatcacatt ttatcaggat atccttctgc
     8461 gaggttatta tgcttcctgt aaataatccc cccctatcca ctggaaacgt ctctttttac
     8521 agaactacat caatcgacaa tgttcacaat aattatctct ccgaatgggt tgaatggact
     8581 aaaaacagca tttccggaga aaacagggaa actgctttta cccggctcca attatgtctg
     8641 gagaacagtg aaacatcgtt ggacttatct tgtttaggtc tcagatctct accacgattg
     8701 cctgacaatc ttgatgaaat taatgtaagc aataaccaac tatcaatgct ccccgagcta
     8761 ccaagggcat tgaaagagct gaatgcaagc agtaatcaat tatctgcact tcctgaatta
     8821 ccagtgtcgc tggaatatat aaatgtgagt gataaccatt tgttcgcact tcctgaatta
     8881 cctgcgtcac tagaatatat taatgtaagt gacaatcacc tgtctgtact tccgaggtta
     8941 ccaatgtcat tggaattact tgatgcagcc agaaatgctt tggaagtaat accagatttt
     9001 ccagaaagag atgatcatat tataagaata ttctggctta atcagaaccg gatcacggca
     9061 attccggaaa gcatacttgg cctcagttct gatagcgttg tcaatcttag agaaaatcaa
     9121 ctatctccca gaataatgca aactttgtta caacaaaccg ccca
//
LOCUS       DAJVPT010000133         9160 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVPT010000133 DAJVPT010000000
VERSION     DAJVPT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            65..721
                     /gene="pdxH"
                     /locus_tag="OQM47_003427"
     CDS             65..721
                     /gene="pdxH"
                     /locus_tag="OQM47_003427"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1481450.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            850..2124
                     /gene="tyrS"
                     /locus_tag="OQM47_003428"
     CDS             850..2124
                     /gene="tyrS"
                     /locus_tag="OQM47_003428"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1481451.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2183..3046
                     /gene="pdxY"
                     /locus_tag="OQM47_003429"
     CDS             2183..3046
                     /gene="pdxY"
                     /locus_tag="OQM47_003429"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1481452.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3090..3695)
                     /gene="gstA"
                     /locus_tag="OQM47_003430"
     CDS             complement(3090..3695)
                     /gene="gstA"
                     /locus_tag="OQM47_003430"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1481453.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3801..5303)
                     /gene="dtpA"
                     /locus_tag="OQM47_003431"
     CDS             complement(3801..5303)
                     /gene="dtpA"
                     /locus_tag="OQM47_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1481454.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5914..6549)
                     /gene="nth"
                     /locus_tag="OQM47_003432"
     CDS             complement(5914..6549)
                     /gene="nth"
                     /locus_tag="OQM47_003432"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1481455.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6549..7244)
                     /gene="rsxE"
                     /locus_tag="OQM47_003433"
     CDS             complement(6549..7244)
                     /gene="rsxE"
                     /locus_tag="OQM47_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1481456.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7248..7868)
                     /gene="rsxG"
                     /locus_tag="OQM47_003434"
     CDS             complement(7248..7868)
                     /gene="rsxG"
                     /locus_tag="OQM47_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1481457.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7872..8930)
                     /gene="rsxD"
                     /locus_tag="OQM47_003435"
     CDS             complement(7872..8930)
                     /gene="rsxD"
                     /locus_tag="OQM47_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1481458.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(8931..>9160)
                     /locus_tag="OQM47_003436"
     CDS             complement(8931..>9160)
                     /locus_tag="OQM47_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1481459.1"
                     /translation="KLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPE
                     EQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         2324 a         2303 c         2334 g         2199 t
ORIGIN      
        1 cgctgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
       61 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
      121 aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg aacgttggct
      181 ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg ctaccgtgga
      241 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
      301 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
      361 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
      421 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
      481 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
      541 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
      601 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
      661 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
      721 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
      781 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
      841 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
      901 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
      961 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     1021 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     1081 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
     1141 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
     1201 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     1261 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     1321 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     1381 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct gcaaattggt
     1441 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     1501 aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac caaatttggt
     1561 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     1621 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
     1681 atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg taaagcaccg
     1741 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
     1801 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
     1861 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     1921 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     1981 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     2041 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     2101 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     2161 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     2221 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
     2281 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
     2341 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     2401 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
     2461 cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc
     2521 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     2581 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     2641 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattga
     2701 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     2761 ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc cactggtgga
     2821 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     2881 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga
     2941 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
     3001 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
     3061 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
     3121 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
     3181 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     3241 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
     3301 ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac
     3361 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     3421 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     3481 tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc
     3541 acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     3601 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     3661 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     3721 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
     3781 taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc tgcagcgtca
     3841 tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca
     3901 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg
     3961 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
     4021 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     4081 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
     4141 cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc
     4201 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
     4261 ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
     4321 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
     4381 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
     4441 aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     4501 ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
     4561 gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca
     4621 acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa
     4681 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     4741 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct
     4801 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     4861 agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     4921 aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca
     4981 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     5041 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     5101 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     5161 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     5221 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
     5281 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     5341 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     5401 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     5461 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     5521 aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg cggcgatatg
     5581 ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
     5641 atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt
     5701 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
     5761 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
     5821 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
     5881 tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct ctttgtattc
     5941 acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg
     6001 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag
     6061 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
     6121 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
     6181 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
     6241 ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct
     6301 gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta gttcaagcat
     6361 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgatatc
     6421 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
     6481 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc
     6541 tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca
     6601 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag
     6661 ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
     6721 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg
     6781 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta
     6841 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
     6901 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
     6961 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
     7021 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
     7081 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
     7141 gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg
     7201 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
     7261 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
     7321 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
     7381 tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg
     7441 gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc
     7501 tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc
     7561 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
     7621 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag
     7681 caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgtc
     7741 ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca
     7801 gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt
     7861 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
     7921 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
     7981 aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga
     8041 ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca
     8101 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
     8161 gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
     8221 tgccatagca accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg
     8281 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg
     8341 cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
     8401 cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg
     8461 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
     8521 accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc
     8581 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc
     8641 atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct
     8701 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
     8761 gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac
     8821 cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
     8881 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
     8941 tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact
     9001 gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc
     9061 ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg atcaacctgt
     9121 tcttctggct ccgcattggc ctgttgctgt tccagcttgc
//
LOCUS       DAJVPT010000134         9159 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVPT010000134 DAJVPT010000000
VERSION     DAJVPT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            215..1348
                     /locus_tag="OQM47_003437"
     CDS             215..1348
                     /locus_tag="OQM47_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481460.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2027..2245
                     /locus_tag="OQM47_003438"
     CDS             2027..2245
                     /locus_tag="OQM47_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1481461.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2463..2705
                     /locus_tag="OQM47_003439"
     CDS             2463..2705
                     /locus_tag="OQM47_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481462.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3035..3964)
                     /gene="fdhE"
                     /locus_tag="OQM47_003440"
     CDS             complement(3035..3964)
                     /gene="fdhE"
                     /locus_tag="OQM47_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1481463.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3961..4596)
                     /gene="fdoI"
                     /locus_tag="OQM47_003441"
     CDS             complement(3961..4596)
                     /gene="fdoI"
                     /locus_tag="OQM47_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1481464.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4593..5495)
                     /gene="fdoH"
                     /locus_tag="OQM47_003442"
     CDS             complement(4593..5495)
                     /gene="fdoH"
                     /locus_tag="OQM47_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1481465.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5508..8558)
                     /gene="fdnG"
                     /locus_tag="OQM47_003443"
     CDS             complement(5508..8558)
                     /gene="fdnG"
                     /locus_tag="OQM47_003443"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7971..7973),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1481466.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8752..>9159
                     /locus_tag="OQM47_003444"
     CDS             8752..>9159
                     /locus_tag="OQM47_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1481467.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPV"
BASE COUNT         2276 a         2239 c         2340 g         2304 t
ORIGIN      
        1 aacagttata atattgaccc tctttgtgaa tgcttcaagt attaatcaaa cttacttaca
       61 tgataaaaat cctggtgacg ttattagcct gtatggcact ttagataaca ttccccttgt
      121 agttaaggaa tgcacgggta aaaatcatca aattaatgtt aaagccaaat tgccacaaga
      181 acgaagtgaa aaagttgagg atatgtgtga aagaatgaaa ggtggcatct ctatgtttaa
      241 ccacaccaca aaaacagctg ataatataga gcataatttg cagctaagtt ttctcgtggg
      301 ttcacgtccg tcgatcacca ataaatactc agcaaaagat gtaggtccag atatacttgc
      361 attgcccgtc aacgtatatc atgcggattt taaaattaaa atcaataatg cagtggagaa
      421 ggcttcatca attgtgttat gtgctgtgca aaaaaatctc catgcagcaa ggaaggttct
      481 tagcgggata ataggaaatg tgccaaaaaa taataaatca aaaacaatta tttttgattt
      541 gcgttttctt agtgcaatta agcagaaagt ttttttgggc agtgaaaaac cgttgtttgt
      601 aaagcatacc gcaatgatga gggaggcatg taaggaatta ccgcagtcag tggaatatgt
      661 tcctttagcc tgtgaagcgc atagccatcg cagcgtcgcc cttgccaaaa ccgtggctgc
      721 gcccatcatg gcgacaggtg tgggcttggc attaatgtat gcaacatcat caggaccgta
      781 ttatgcgcaa agtctttctg gttcgccgga tatcgagatg ataaaaaatc gcatggctga
      841 attgtttaac cagtcggata tttctgtgcg caacaaatat agcccggctt ttgataaatg
      901 gaatgaactg gtagataaat taaatcacga aagaaataca gttccgtttg cgtgtttgac
      961 caccatttta gcgacggcga taaatgaaac tcttgagggg gatacgaatg ttgccgttgt
     1021 gatggggtgt aaatctgcga aagatcgcac gatatccatt gtccttggca actcgatgtt
     1081 gcaaacactc cttgaaaaac gcctcgccga tagtggagaa atagaaaatc tttttgatca
     1141 acagggatat tttagttgcg attcattaac acccgaagaa ttaatgatgt taaaagacct
     1201 ttttgatatt cgagttcttc atgtctctaa caaattcaat gttggattgc aaggtaatat
     1261 caacactgat gttttacaag attcgttttt taaaaatgtc gatttcattc atgaatataa
     1321 ctcgtatacc gtagggatgg gagtctgacg agtaaaactc attaggatta tcagaattcg
     1381 gacaatgcat tgcaaaaatg acatacaaag agagatgtgt gcaggtactt agccgcgagg
     1441 ggcatggcga agccatacag aaagtaccag tattcagaac tccagtgaaa gcggcatagt
     1501 ggccgcctgc tcaggagttt tgctatgacc cgtaatcgtg ctgaacgtcg ccaccctatg
     1561 atgcgattaa aaaaactcat cgtcgcgctg atgattgacg tcaatgctat cggatgatgc
     1621 cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga
     1681 tgagcaagta aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc
     1741 accttaaatc ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg
     1801 taaataaata tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa
     1861 ctaaaccatt tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac
     1921 gcctcgcaaa aacaaaatga catgcaaaaa tccgcatttt tttaaatgct tttccggcgt
     1981 aattttcgca catacattaa tgagtaatat atgtaaggtg gtggtaatgg cgatgaatac
     2041 ggtttttctt catttatcag aagaggcaat taaacggctg aacaagcttc ggggctggcg
     2101 taaggtttca cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca
     2161 atttccggtg cgtaaagcaa agggaggcag gcaaaagggg gaggtagttg gtgttgatga
     2221 tcagtgtaag gagcataagg aatgaaaaaa atttttcatt cttgaatata aaaaacagat
     2281 gcccttattc tggtattaat acaaggctgt tttacttgaa cttataataa ctgcaactgt
     2341 tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt
     2401 taaagattcg gcaagggtac ttttctctcc atagattcaa aaatgatatt ggcgagatat
     2461 ttatgaactc tctggcaggt atagatatgg gcagaatttt actcgattta tcgaatgagg
     2521 tgattaagca acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa
     2581 gggaagcggt agatcaatac ctgataaatc aatcgcaaac agcaagaacc agtgttcctg
     2641 gcatctggca agggtgtgag gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat
     2701 ggtaatggga aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg
     2761 atgcggcgtg aacgccttat ccggcctaca aagtcatgca aattcaatat attgaagttt
     2821 attggtaggc ctgataagcg cagcgcatca ggcaatcata cgcttgtcat cagtgacata
     2881 cccataacct tatcgatgtg ttggagggaa gaacgaggca aacagacaat tgcggcatga
     2941 tcttgaatgg gtaacgcgga atacgagaga tggtgtcgtc acaccctatg agctgtaatc
     3001 cgggcgacct gcgccacccg gaaaacgtca gagattactc tccttcaccc ggaaacagga
     3061 acgggttaat ggaactacgg gcgtagcctt cttgctccat tcgcgcgtcc agtaccagag
     3121 aggcgaggtc atctgccacg gcttcaactt tcggttcttt ttcctgataa agaatcttca
     3181 ggtaagtgcc gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg
     3241 accagtaatg cagtttgccg ctctgctcgc agttgctgca ttttacgcgc actacgtgcc
     3301 attcggtttc acacaggttg cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca
     3361 ccatgctgga caccggcata gagccacata ccgggcaata ttgacgttgt tcgccgtatt
     3421 cagcgcgggc tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagcgcag
     3481 cccagataaa cggcgcttta tcgctgctga cggacgagaa atcagaggca aacagcgcgc
     3541 tggccatatc ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg
     3601 ccgggccgct catttcaggt ttcagctcag caattagcgc catcagcagc ttttgccagt
     3661 gcttatcacg cggcagaacg tgaatatcca gcgggggctt gccctgtgcg ctggcttctt
     3721 taatgcgcgc ggtcagatcc atctccagcg gatggtcgta cagcaccact ttctgggcgt
     3781 gggcgataag cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc
     3841 gcagacgctc ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa
     3901 tcatatccgc cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac
     3961 tcattcagct ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt
     4021 cttcgcccag gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca
     4081 aagggcggcg tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg
     4141 cagcattaac gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca
     4201 gataatcacg ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca
     4261 tttctgaccg aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg
     4321 aatattcttc gcccaaaaga tatcatcccg attgattagg ttttgatgcc agtaacggaa
     4381 aaacatgatg atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc
     4441 cagctgcggt gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag
     4501 cccgctcacc gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg
     4561 cgcggtgtag cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg
     4621 tgcagattat tctcttcctc atccgcacgg ttcggaccga cacccacgta gtggaagata
     4681 ctggccgcaa aggtagccgc aaagccaaca gccgcgagcg gtttccagat gcctttccag
     4741 aatttcacgg tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg
     4801 tcagcatggt gcagcacgta catgacgtgt gtaccaccga cgcctgccgg atcgtacaga
     4861 cccgcattgt cgtaaccgcg ggttttcagc tcagcaacgc gctcgctcgc cagcgttttc
     4921 atcgactctt tcgtgccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt
     4981 tcttgcccaa ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc
     5041 gggttgaggc gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac
     5101 tgctcggact ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg
     5161 cacgctttca ggcagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat
     5221 tccagtttgt cgttctgctc cacttccgag aagcgcatta ccgtccacga tttggcgctt
     5281 aaatcattgg ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc
     5341 cactctgaac acgccacctg acaggcttta cagccgatac aggtggtaac gtcgatgagt
     5401 ttcgccactt cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg
     5461 gaacgacgaa tgatatcttg agattgataa gccatatgtc gtctccgtta caccttttcc
     5521 acattcacaa ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc
     5581 gttaaagtat tggcaataaa gcctttttta gctacgcctt catagcccca gtgaatcgga
     5641 ataccgatgg tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc
     5701 accgctttgg ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca
     5761 atgccaagtt tattcgccag cgactccccg atttccacaa actgctctgg ctgcaaaatc
     5821 gcgttcaaca gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt
     5881 ccgacatacg ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt
     5941 gcagctgggt tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac
     6001 ggctcgtagt gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc
     6061 atgccttcct gctgcatgat aaacggcccg acgccgctac ccggaggcgc tgcgctgtag
     6121 tccggaatat cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga
     6181 tcccacgggt taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc
     6241 ggccatgccc atgcccagcc cagcgtgtta ccgaggccag acggatcggc gttatcacga
     6301 cgcgccatct ggttgccttc cggcgtccag ctaccggcga aaatccagca gccacaggag
     6361 gttgtaccgt catcgcgcaa ttgggcgaac gagctaagtt gttggccttt cttgacgata
     6421 accgcaccgg ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgct
     6481 acttcttccg acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctga
     6541 tccgggttcg cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag
     6601 aggatctcac cgtcagtcag cgcaatcccc ggggcgtccg cacctttcca gtgccactgc
     6661 aaccagcgac ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga
     6721 cggaacactt cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc
     6781 cagaagttag aggtttcagt gttcagtggg tcgatggtga cgaggaactt cagtttcgac
     6841 agacaaccga tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag
     6901 atatagccat tgaccttgcc ctctttcatc atctcgaagt attgcaggac gtcgtagcct
     6961 ttatcccact tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc tttatcacca
     7021 aagaaggcct tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttacctgg
     7081 ccttccagca gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc
     7141 tcgcttggca gcgtcatgta acctggcaga ctctgcgaca gtagccccag gtccgtcagc
     7201 ccctgaatat tggagtgacc gcgcagggcg ttaacgccgc cgccggccat ccccatattg
     7261 ccgagcagca gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc
     7321 gtccagccga gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg
     7381 tattcgcaga ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct
     7441 ggcgtgtagc gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc
     7501 gtggtatcgc gtttggcgaa gccgttttcg tccagttcat aagtccagga ggatttatcg
     7561 tacttgcgtt tttccgcgtc gtagccggtg aacaggccat cttcaaagcc gtaatcctca
     7621 cgcacgatca ggctggcgtt ggtataggct tcggtgtatt cgtggttgaa tttttcattg
     7681 ttcagcaggt acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg
     7741 gcatagtagt cagctaccgc cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc
     7801 gcgccgttgt gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg
     7861 gcgttaccgc ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc
     7921 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc
     7981 gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg
     8041 gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc
     8101 acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg
     8161 atgcgatcaa acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg
     8221 tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc
     8281 ggacaaagtg caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat
     8341 gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa
     8401 caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc
     8461 gcgagtgcta cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca
     8521 gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat
     8581 tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt
     8641 gcggaagggg ccgcaaaggt ccagaattgg ctcaatttcc ctccatctag gaagggtttg
     8701 taacagaata ccataatgtt ggtgtgtatg ttcttatctg gttaagagaa agtgaaaaaa
     8761 acacagcgaa aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta
     8821 tggcggcgtg atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt
     8881 gcgctggtct acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag
     8941 tactttgcgc tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc
     9001 ggcatggatg tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc
     9061 cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta
     9121 tgcggcgtgg agcaacttaa tgacatcgga aaaccggtg
//
LOCUS       DAJVPT010000135         9055 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVPT010000135 DAJVPT010000000
VERSION     DAJVPT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..723
                     /locus_tag="OQM47_003445"
     CDS             <1..723
                     /locus_tag="OQM47_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1481468.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRT
                     ALRAVRDTVVSVARAVWNWIFF"
     gene            1057..4179
                     /gene="ag43"
                     /locus_tag="OQM47_003446"
     CDS             1057..4179
                     /gene="ag43"
                     /locus_tag="OQM47_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1481469.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            4300..6816
                     /locus_tag="OQM47_003447"
     CDS             4300..6816
                     /locus_tag="OQM47_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481470.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            6892..7347
                     /locus_tag="OQM47_003448"
     CDS             6892..7347
                     /locus_tag="OQM47_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481471.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7466..7677)
                     /locus_tag="OQM47_003449"
                     /pseudo
     CDS             complement(7466..7677)
                     /locus_tag="OQM47_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7758..8576
                     /locus_tag="OQM47_003450"
     CDS             7758..8576
                     /locus_tag="OQM47_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1481472.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            8918..>9055
                     /locus_tag="OQM47_003451"
     CDS             8918..>9055
                     /locus_tag="OQM47_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1481473.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QW"
BASE COUNT         2136 a         2407 c         2545 g         1967 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg ggccctgtct
      241 gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg tattcagcct
      361 tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac ccgactgcag
      541 gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg
      661 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gatcttcttc
      721 tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa gcgctgtttt
      781 cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga tcgataccta
      841 ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag caacgtgcgc
      901 agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa acttccgtca
      961 gccggacctc cggcactgta accctttacc tgccggtatc cacatctgtg gataccggct
     1021 tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct gaatacctgc
     1081 tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga actggcccgc
     1141 gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt cacgtcactc
     1201 ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg cggaacactg
     1261 gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt cagtaccggg
     1321 cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa agcgggtggc
     1381 acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc aggaggaact
     1441 gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact ggcggtgaac
     1501 accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc tgccggtaca
     1561 attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac cggaaccatc
     1621 gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg tcagatggtc
     1681 ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat ctggtcttcg
     1741 gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca cggagaggca
     1801 cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac ggcaacagag
     1861 acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc cgcgcatacc
     1921 accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc tgatgtcacc
     1981 cagaacacgg gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg cacaaaccgc
     2041 ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga aaatggcgga
     2101 cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga tggcggaacg
     2161 ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg cggtgtactg
     2221 ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc attcagtatc
     2281 ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac gctgaacgcc
     2341 ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag gggcggcaca
     2401 ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg gaagacggtg
     2461 aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg cgctctcacc
     2521 ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa caccacactc
     2581 acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag taccgtcacc
     2641 acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac tgtgctgaac
     2701 ggtgccattg accccacgaa tgtcactctc gcctctggtg ccacctggaa tattcccgat
     2761 aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat tcatttcacc
     2821 tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct gaacggacag
     2881 aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc tgacagactg
     2941 gtcattgacg gtggcagggc aaccggaaaa accatcctga acctggtgaa cgccggcaac
     3001 agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc cattaacggt
     3061 gccaccacgg aggaaggggc ctttatccag gggaataagc tgcaggccgg tgcctttaac
     3121 tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa tgcttatcgt
     3181 gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga ccggattctg
     3241 gcaggctccc gcagccatca gaccggtgta agcggtgaaa ataacagcgt ccgtctcagc
     3301 attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg agccacgccg
     3361 gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag aacagaggtt
     3421 gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc cgttgatgtt
     3481 aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag cctgggcgga
     3541 tacctgaatc tgatacacaa cgcctccggc ctgtgggctg acattgtggc ccagggaacc
     3601 cgccacagca tgaaagcctc atcggacaat aacgacttcc gcgtccgggg ctggggctgg
     3661 ctgggttcgc tggaaaccgg tctgcccttc agtatcactg acaatctgat gctggagccg
     3721 caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa cgccagttat
     3781 gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct gggcagccac
     3841 cacgatatga actttggtaa aggcacctca tcccgtgaca ccctgcgcgg cagtgccaaa
     3901 cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttctgttat ccgcaccttc
     3961 agctcccggg gggacatgag catgggtaca gccgcagccg gcagtaacat gacgttctca
     4021 ccgtcacaga atggtacgtc actggacctg caggccggac tggaagcccg tgtccgggaa
     4081 aatatcaccc tgggcgttca ggccagttat gcccacagca tcaacggcag cagcgctgaa
     4141 ggttataaca gtcaggccac actgaatgta accttctgat aattctgcat tgcctctctg
     4201 tggtcccggt catgatgacc gggacccgga cgggggaaaa cgtttcagtg ccacattcac
     4261 tggcattcac aataacatga tattcatcac ggagtgacta tgttacagat agtcggcgcg
     4321 ctgattctgc tgatcgcagg atttgccatt cttcgccttt tgttcagagc attaaccagc
     4381 acagcgtctg cgctggcagg gttcatattg ctgtgtctgt tcggtccggc tttactggct
     4441 ggctatatca ctgaacgcat aacccggtta ttccatattc gctggctggc aggcgtattt
     4501 ctgacgattg ccggaatgat catcagcttc atgtggggac ttgatggtaa acatatcgca
     4561 ctggaggctc atacttttga ctctgtaaaa tttattctga ccaccgctct cgccgctggt
     4621 ctgctggctc ttcccgtgca gataagaacc attcagcaga acgggctcac acctgaagat
     4681 atcagcaagg aaattaacgg gtattactgc tgtttttata ctgctttttt ccttatggcg
     4741 tgttctgcat acgcaccatt gatcgcattg cagttcgata tttcaccctc actgatgtgg
     4801 tggggcgggt tgttgtactg gctggctgca ttagtgacgc tgctatgggc ggccagccag
     4861 atccaggcgc tgaaaagact gaccagtgcc atcagccaga cactggaaga acaaccggtg
     4921 ctcaacagta aatcgtggct gagcagtttg caaaacgatt acagccttcc tgaaacgctg
     4981 acggagcgca tctggctgac actcatttca caacggattt cccggggaga actgagggaa
     5041 tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag aaacatggca
     5101 gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact gaaaacgctc
     5161 ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg
     5221 gacggcggtg actggtaccc cttttcagaa ggccgccgtt ttgtatcatt ccaccacgtg
     5281 gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat
     5341 cataagccgg ctccggaatg gtactgctcc tctctttgtc acgaaacaga aacactgtgt
     5401 caggacattt atgaacgttc ttacaccggt tttatttccg atgcaacggc gaatggtctg
     5461 attctcatga aactgccgga aacctggagt acaaatgaga aaatgtttgc ttccggaggg
     5521 cagggacatg ggtttgccgc tgaacgggga aaccatattg tcgacagagt ccgtctgaaa
     5581 aacgcacgga tcctcggtga taataatgcc agaaatggag cagacagact ggtcagcgga
     5641 acagaaatcc agacgaaata ttgttcaact gcagcccgta gcgtcggtgc ggcattcgac
     5701 ggacaaaacg gacagtatcg ttacatggga aatcatggtc ccatgcaact ggaagtcccc
     5761 cgtgatcagt atgccggcgc tgtggaaacc atgaagaata agatccgcga aggtaaagta
     5821 cccggtgtaa ccgatcccgc agaagcgtcc cggctgattc gtcggggaca tctgacttat
     5881 acccaggccc gtaatatcac ccggttcggg accatcgaat cggtcactta tgatattgcc
     5941 gaggggtcgg ttgtcagtct ggcggccgga gggatcagtt ttgccctgac ggcatcggtc
     6001 ttctggctca gcaccggcga tcgcgatgct gccctgcaga cagctgctgt ccaggcagga
     6061 aaaaccttca cccgcacact ggctgtctac gtcacaaccc agcaacttca ccggctcact
     6121 gttgttcagg gtatgctgaa gcatattgat ttttcgacgg ccagcccgac tgtccggcag
     6181 gcgcttcaga aggggaccgg tgcaggaaat atcagtgccc tgaacaaagt gatgaagggt
     6241 acgctggtga catctctggc actggtagct gtcacaacca gccctgacat gatcaaaatg
     6301 ttgcggggac ggatctccgg tgcgcagttc atcaggaatc ttgccgtggc atcttccggt
     6361 gtggcaggtg gtgctgtcgg gtcagtggcg ggcgggatat tgttcagtcc actgggacca
     6421 tttggtgcac tgacagggcg tgtggttggc ggtgttctgg ggggaatgat tgcctccgct
     6481 gtatcaggaa aaattgccgg agcgctggtt gaagaagatc gcgtcaaaat tctggcaatg
     6541 attcaggagc aggtgacatg gcttgccggc agtttcctgc tgaccggaca tgagattgaa
     6601 aatctgaacg cgaatctggc ccgtgttatc gatcagaatg ctctggagat cattttcgcc
     6661 gccggtatac aacaacgggc cgcgaccaat atgttaatca aaccactggt ggtcagtatc
     6721 atcaggcaac gccccgtcat ggaatatgat gcatcccatc tcggcaagat ggttaaccgg
     6781 ctggaagaag cattcccccc ggagctcccg gcataacgtg tgttctgctc cttttaggtt
     6841 ttatcacctg atttgccagt gtcatcgtca tgactcagag aggtaaatac catgattcac
     6901 ctgttcaaaa cctgcatgat taccgccttc attctggggt taacgtggtc tgccccactc
     6961 cgggcacagg atcaacgtta catcagtata cgcaatacag atacgatatg gctcccggga
     7021 aatatttgtg cttaccagtt ccggctggat aatggcggaa acggtgaagg atttggcccc
     7081 ctcaccatca ctctgcaact caaagacaaa tatggtcaga cgctggtgac cagaaaaatg
     7141 gaaacggaag cttttggtga cagtaatgcc acgcgaacca cagacgcatt tctggaaacg
     7201 gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga aagtaacggc
     7261 catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc actgctgatt
     7321 accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt cagcctctgc
     7381 cacaacgtat ggcagaggct atttatcagg agaaaccaga tatgccgggt tacacacaga
     7441 gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca taaccgctta
     7501 ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta tccgcaacgc
     7561 ggaaggtcag cgacgctgac gcagccggaa ctctgaagct gatgccggaa aacagcttaa
     7621 tgcgtggctc gccagtggag ggcttctcag acaataaact tcattgattt catccatcag
     7681 gccgcgtctt ctccgggaga cgcggctttt ttcatttgtt gcaccagtaa atcttaacca
     7741 ccgataagga gcaaagtatg caattagtca gtcgttttgg ttatgcgaac cagatacgcc
     7801 gtgaccgtcc gctgacacat gaagagctga tgcaccatgt gccggggatt ttcggggaag
     7861 agaaacacac gtcccgaagc cagaactata cgtacatccc caccatcacc gtactggaaa
     7921 gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca gacccgtgtg cgcgacccgg
     7981 gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag
     8041 aacatgtccc tgaaattatt ctgctcaact cccatgacgg tacctccagc taccagatgc
     8101 tgccgggtta cttcagattc gtctgccaga acgggtgtgt ctgtggtcag tctctggggg
     8161 aagtgcgtgt tccgcaccgg ggaaacgtgg tggagaaagt tattgaaggg gcttacgaag
     8221 tggtgggcgt gtttgaccgg attgaggaga aacgtgatgc catgcagtcg ctggtcctgc
     8281 cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac
     8341 atcagcccgt caccaccgcc gacattctga cgccacgacg ccgggaggat tacggtaagg
     8401 acctgtggag tacttatcag accatccagg agaatatgct gaaaggcggg atttccggcc
     8461 gcagtgcaaa aggaaaacgt atccacaccc gtgccattca caacatcgac accgatatta
     8521 agctcaaccg cgcattgtgg gtgatggcag aaacgctgct ggagagcctg cgctgatgca
     8581 gcatgtccct gaaggcgtga ttcaggcacc agaatcaccc gacgccccgg aggcgctgcc
     8641 ggggtgtcgt tccatttctg atgatgacct gtgcgcctgg tgtacccgtc tgctttaccg
     8701 tcccggagaa atcagtctgt gcagactgag tatacaggca atggcatctg gccatccgtc
     8761 tgtgaccgga acggttacgc gtacggctgt ccggaatttc agccgaatat cattcatccc
     8821 tgatattacc gggtcatcac ggtcagtgat gaacgtgatg ttgccagctc ccgaccttta
     8881 ctccctctca tttattcaca ttacaaggat ttcatacatg aaaacattgt ctcagaacac
     8941 cacctcttcg gcctgtgcac cggagactga cctgcaacaa ctggttgcca ccctcgtccc
     9001 tgatgaacag cgcatcagct tctggccgca gcactttggc ctcattccac agtgg
//
LOCUS       DAJVPT010000136         9020 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVPT010000136 DAJVPT010000000
VERSION     DAJVPT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(88..1464)
                     /gene="trkA"
                     /locus_tag="OQM47_003452"
     CDS             complement(88..1464)
                     /gene="trkA"
                     /locus_tag="OQM47_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1481474.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1486..2775)
                     /gene="rsmB"
                     /locus_tag="OQM47_003453"
     CDS             complement(1486..2775)
                     /gene="rsmB"
                     /locus_tag="OQM47_003453"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1481475.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2821..3768)
                     /gene="fmt"
                     /locus_tag="OQM47_003454"
     CDS             complement(2821..3768)
                     /gene="fmt"
                     /locus_tag="OQM47_003454"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1481476.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3783..4292)
                     /gene="def"
                     /locus_tag="OQM47_003455"
     CDS             complement(3783..4292)
                     /gene="def"
                     /locus_tag="OQM47_003455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1481477.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4457..5581
                     /gene="dprA"
                     /locus_tag="OQM47_003456"
     CDS             4457..5581
                     /gene="dprA"
                     /locus_tag="OQM47_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1481478.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5553..6026
                     /locus_tag="OQM47_003457"
     CDS             5553..6026
                     /locus_tag="OQM47_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1481479.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6055..6597
                     /locus_tag="OQM47_003458"
     CDS             6055..6597
                     /locus_tag="OQM47_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1481480.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6602..7174
                     /gene="tsaC"
                     /locus_tag="OQM47_003459"
     CDS             6602..7174
                     /gene="tsaC"
                     /locus_tag="OQM47_003459"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1481481.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7179..7997
                     /gene="aroE"
                     /locus_tag="OQM47_003460"
     CDS             7179..7997
                     /gene="aroE"
                     /locus_tag="OQM47_003460"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1481482.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7994..8251
                     /locus_tag="OQM47_003461"
     CDS             7994..8251
                     /locus_tag="OQM47_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1481483.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8227..8781)
                     /locus_tag="OQM47_003462"
     CDS             complement(8227..8781)
                     /locus_tag="OQM47_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1481484.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2253 a         2190 c         2253 g         2324 t
ORIGIN      
        1 gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata
       61 ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct
      121 ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc
      181 aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat
      241 cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc
      301 atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc
      361 aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga
      421 aattgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa
      481 taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc
      541 gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag
      601 ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc
      661 ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg
      721 cgccagccct gcaccgatat taccgccacc aaccagcatg atccgtttat acggtttttc
      781 cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac
      841 ttcatcacca gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa
      901 aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt
      961 accaatcagc gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc
     1021 gaagttcacc acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac
     1081 caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc
     1141 ggcatcgcgt acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag
     1201 tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag
     1261 catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg
     1321 cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac
     1381 aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc
     1441 ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact ttttgattag
     1501 cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc tgattttgtt tacccggttg
     1561 ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aggctttaat
     1621 ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga
     1681 ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat
     1741 atcgcgatcg cgacgtaacc atttaatatc tggatggcga cgaatcaccc ccgttgctga
     1801 acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta
     1861 acggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaat
     1921 gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc
     1981 aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaagatgt gttcaccgtt
     2041 ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc
     2101 gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta cagcatcagg
     2161 gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc
     2221 gcgggaatga tgcgtacgat taatacgcag ccacattggc ggacgctggt tattggcttc
     2281 ggcgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg
     2341 atgcagataa cgtgcatcac gggtattaaa ctcggctaat aactcttctt gccgacgctg
     2401 gaactggcgt aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc
     2461 gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata
     2521 caaaccaacc ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat
     2581 taacttatta atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc
     2641 ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca gaatgttgct
     2701 taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt
     2761 acgttgtttt ttcataagta taaaaatgct cggcaagacc gggcttagaa gagtggacta
     2821 tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga ggtcttgcgc
     2881 gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc catcaccagt
     2941 cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc
     3001 cgtatcaatg accgacgctt tccagacttt aaccggctgt ccttcaattt ccagccagct
     3061 cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg aaagtgacca
     3121 gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag tttcgtcctg
     3181 aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa gcccttgtgg
     3241 gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca
     3301 ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca taatggttac
     3361 accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg
     3421 tggcagcagt gaaccatgaa tgttgataca gccaagacgc ggcatctcca gcacagcttt
     3481 aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcaaat cggcgaccag
     3541 ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac cttcttcctc
     3601 agccagaact ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg
     3661 ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc
     3721 tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat
     3781 ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat tcgttgttgt
     3841 ttcagcggtg acagataatc cataaacagt ttgccaacca ggtgatccat ctcatgctga
     3901 atacagatag ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc gcggtcaagg
     3961 gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg gatcgacagg
     4021 caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc
     4081 agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc
     4141 tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg
     4201 atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg
     4261 tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta
     4321 gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat cacttgattg
     4381 acagcatcac ttgattgaca gcatcactga ccaatcgcaa agattgctaa ggcttgctta
     4441 tggcagggag ataaggatgg tcgatataga aatttggctg cgtttaatga gcatcagcag
     4501 cttgtacggc gatgatatgg tccgtatcgc tcactggctg gcaaaacagt cgcatattga
     4561 tgcggttgta ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
     4621 tccacgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
     4681 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
     4741 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
     4801 tcgggcgcat tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac
     4861 gtgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg tgcataaagc
     4921 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
     4981 tccgcgtcgc catgcccgac tggctgccag tctgtttgaa cagggtggcg ctctcgtctc
     5041 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
     5101 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
     5161 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
     5221 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
     5281 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
     5341 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
     5401 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
     5461 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
     5521 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
     5581 aaacctatat tcacactgaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
     5641 ttaccgacgc aggttttgag cgagaagata tctacaatgc cctgctatgg ctggaaaaac
     5701 ttgctgatta tcaggaaggg ttggcagaac cgatgcaact ggcctcggat cctctctcca
     5761 tgcgtattta tacaccggaa gagtgtgaac gactggatgc cagctgccgt gggtttctgc
     5821 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
     5881 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggtg atcctgatgg
     5941 tgttgttcaa catcccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
     6001 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggca
     6061 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
     6121 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
     6181 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
     6241 gtttgccccg tatgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
     6301 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
     6361 gcaattacat gcccccaatg tcggacgggc catctggtcc agcgccgctc ccgttatggc
     6421 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaacca
     6481 atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag
     6541 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
     6601 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa
     6661 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
     6721 aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
     6781 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgt tgactgacgt
     6841 gcagcgtgaa accatttttt cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc
     6901 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
     6961 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
     7021 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
     7081 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
     7141 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
     7201 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
     7261 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
     7321 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
     7381 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgca gttaataccc
     7441 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa
     7501 gcgatctgga acgtctgtct tttatccgcc ctggtttacg tattctgctt atcggcgctg
     7561 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
     7621 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
     7681 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
     7741 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
     7801 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
     7861 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
     7921 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
     7981 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agggaagagt gggacgagaa
     8041 taaaaaatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat gcgcgatctc
     8101 tggcgagagt ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt
     8161 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
     8221 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
     8281 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
     8341 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
     8401 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
     8461 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
     8521 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
     8581 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
     8641 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
     8701 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
     8761 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
     8821 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
     8881 tgcagcaaaa agggtgaaaa aacaacaaac agaaaagaag atcaaaaaaa tacttgtgca
     8941 aaatattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
     9001 atggtcggaa caacgaagag
//
LOCUS       DAJVPT010000137         9016 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVPT010000137 DAJVPT010000000
VERSION     DAJVPT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..189)
                     /locus_tag="OQM47_003463"
     CDS             complement(<1..189)
                     /locus_tag="OQM47_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1481485.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNG"
     gene            complement(325..1116)
                     /gene="aadA1"
                     /locus_tag="OQM47_003464"
     CDS             complement(325..1116)
                     /gene="aadA1"
                     /locus_tag="OQM47_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1481486.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(1229..2059)
                     /locus_tag="OQM47_003465"
     CDS             complement(1229..2059)
                     /locus_tag="OQM47_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1481487.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2269..3282
                     /gene="intI1"
                     /locus_tag="OQM47_003466"
     CDS             2269..3282
                     /gene="intI1"
                     /locus_tag="OQM47_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1481488.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3485..3835
                     /locus_tag="OQM47_003467"
     CDS             3485..3835
                     /locus_tag="OQM47_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1481489.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTSEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3961..4521
                     /locus_tag="OQM47_003468"
     CDS             3961..4521
                     /locus_tag="OQM47_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1481490.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4524..7490
                     /locus_tag="OQM47_003469"
     CDS             4524..7490
                     /locus_tag="OQM47_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1481491.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7557..7934
                     /locus_tag="OQM47_003470"
     CDS             7557..7934
                     /locus_tag="OQM47_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1481492.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(8135..8794)
                     /locus_tag="OQM47_003471"
     CDS             complement(8135..8794)
                     /locus_tag="OQM47_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1481493.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         2017 a         2538 c         2414 g         2047 t
ORIGIN      
        1 gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt
       61 gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt
      121 gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac
      181 agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
      241 caccgcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg
      301 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
      361 gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
      421 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
      481 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
      541 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
      601 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
      661 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
      721 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
      781 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
      841 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
      901 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
      961 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     1021 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     1081 ttgagtcgat acttcggcga tcaccacttc cctcatgatg tttaacgttt gaaataaggg
     1141 gcgccgagcg ccagcgaggg gagccaaaag cttgcttttg gccgtcccga cttgattgaa
     1201 gggttgggcg attttgccat tagatttttt ataaatttag tgtgtttaga atggtgatcg
     1261 catttttctt ggcttttatg cttgatgtta aattcgaccc caagtttcct gtaagtgcgg
     1321 acacaaaaac atatttatgt cctgatttgc ttataataaa cccttcaaac catccgtttt
     1381 gtaaggttct atttgctgtg aatcctgcac cagttttccc atacagtttt gtactattat
     1441 ccagatcttg tagatacatg ttctctatgg tgttttctat ggctgagttt ttaactggga
     1501 gattgtgatt aataatttta cgcaggaatt gaatttgttc ttctggtgaa atttttaagc
     1561 tactttcgag ccatgcttct gttaatccgt tgtttctttc tttatctcca gagaagtctt
     1621 gatttccata atcaaaatct ttgagataat tcttgatttt atttaatcca attttttggg
     1681 ttatttcttg cgaaacccaa acaacagaaa attgcatcca cgtctttggt gtatgattgc
     1741 tgttccagat ctccattcct ttgggggttt tatcccattt gaatatggtt ttctgatcta
     1801 ttatttccgc atcaaatgcc ataagtgata atgcgatctt gaaagttgaa tctggtgcca
     1861 tttgcgttgc acactttgct ttattgaatt gagcaatttc agcgtttgtg gatgcatcgt
     1921 aaagtaaaaa acaaccttca gttccttcaa ataatggaga tgcaacagta gagatatctg
     1981 ttgatgcact ggcgctgctg tagataatat ttgcaattat taaaaaaata gcgaagttga
     2041 tatgtattgt gtttttcata ataagtattg gtttggtaaa gggcttaatt ttaacggcta
     2101 acaattaatg aggctccggg ttcgcccaac tttgttttag ggcgactgcc ctgctgcgta
     2161 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     2221 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     2281 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     2341 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     2401 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     2461 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     2521 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     2581 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     2641 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc agcttctgta tggaacgggc
     2701 atgcggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg
     2761 atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgttacc cgagagcttg
     2821 gcacccagcc tgcgcgagca gctgtcgcgt gcacgggcat ggtggctgaa ggaccaggcc
     2881 gagggccgca gcggcgttgc gcttcccgac gcccttgagc ggaagtatcc gcgcgccggg
     2941 cattcctggc cgtggttctg ggtttttgcg cagcacacgc attcgaccga tccacggagc
     3001 ggtgtcgtgc gtcgccatca catgtatgac cagacctttc agcgcgcctt caaacgtgcc
     3061 gtagaacaag caggcatcac gaagcccgcc acaccgcaca ccctccgcca ctcgttcgcg
     3121 acggccttgc tccgcagcgg ttacgacatt cgaaccgtgc aggatctgct cggccattcc
     3181 gacgtctcta cgacgatgat ttacacgcat gtgctgaaag ttggcggtgc cggagtgcgc
     3241 tcaccgcttg atgcgctgcc gcccctcact agtgagaggt agggcagcgc aagtcaatcc
     3301 tggcggattc actacccctg cgcgaaggcc atcggtgccg catcgaacgg ccggttgcgg
     3361 aaagtcctcc ctgcgtccgc tgatggccgg cagcagcccg tcgttgcctg atggatccaa
     3421 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     3481 gacaatggag gtggtagccg agggtgtgga aacacccgac tgccttgcgt ggttgcggca
     3541 ggcgggttgc gacacggtgc agggtttcct gttcgccagg ccgatgccgg cggcggcctt
     3601 cgtcggcttc gtcaaccaat ggaggaacac caccatgaac gccaatgaac cgagcaccag
     3661 ttgctgcgtg tgctgcaagg aaatcccgct cgatgccgcc ttcacgtcgg aaggggccga
     3721 gtacgtggag catttctgcg ggctggagtg ctatcagcgc ttccaggcgc gggccagcac
     3781 tgcgaccgaa accagcgtca aaccggacgc ttgtgattcg ccgccgtcag gttgaggcat
     3841 accctaacct gatgtcagat gccatgtgta aattgcgtca ggataggatt gaattttgaa
     3901 tttattgaca tatctcgttg aaggtcatag agtcttccct gacattttgc agggaattcc
     3961 atgactggac agcgcattgg gtatatcagg gtcagcacct tcgaccagaa cccggaacgg
     4021 caactggaag gcgtcaaggt tgatcgcgct tttagcgaca aggcatccgg caaggatgtc
     4081 aagcgtccgc aactggaagc gctgataagc ttcgcccgca ccggcgacac cgtggtggtg
     4141 catagcatgg atcgcctggc gcgcaatctc gatgatttgc gccggatcgt gcaaacgctg
     4201 acacaacgcg gcgtgcatat cgaattcgtc aaggaacacc tcagttttac tggcgaagac
     4261 tctccgatgg cgaacctgat gctctcggtg atgggcgcgt tcgccgagtt cgagcgcgcc
     4321 ctgatccgcg agcgtcagcg cgagggtatt gcgctcgcca agcaacgcgg ggcttaccgt
     4381 ggcaggaaga aatccctgtc gtctgagcgt attgccgaac tgcgccaacg tgtcgaggct
     4441 ggcgagcaaa agaccaagct tgctcgtgaa ttcggaatca gtcgcgaaac cctgtatcaa
     4501 tacttgagaa cggatcagta aatatgccac gtcgttccat cctgtccgcc gccgagcggg
     4561 aaagcctgct ggcgttgccg gactccaagg acgacctgat ccgacattac acattcaacg
     4621 ataccgacct ctcgatcatc cgacagcggc gcgggccagc caatcggctg ggcttcgcgg
     4681 tgcagctctg ttacctgcgc tttcccggcg tcatcctggg cgtcgatgaa ctaccgttcc
     4741 cgcccttgtt gaagctggtc gccgaccagc tcaaggtcgg cgtcgaaagc tggaacgagt
     4801 acggccagcg ggagcagacc cggcgcgagc acctgagcga gctgcaaacc gtgttcggtt
     4861 tccggccctt caccatgagc cattaccggc aggccgtcca gatgctgacc gagctggcga
     4921 tgcaaaccga caaaggcatc gtgctggcca gcgccttgat cgggcacctg cggcggcagt
     4981 cggtcattct gcccgccctc aacgccgtcg agcgggcgag tgccgaggcg atcacccgtg
     5041 ctaaccggcg catctacgac gccttggccg aaccactggc ggacgcgcat cgccgccgcc
     5101 tcgacgatct gctcaagcgc cgggacaacg gcaagacgac ctggttggct tggttgcgcc
     5161 agtctccggc caagccaaat tcgcggcata tgctggaaca catcgaacgc ctcaaggcat
     5221 ggcaggcact cgatctgcct accggcatcg agcggctggt tcaccagaac cgcctgctca
     5281 agattgcccg cgagggcggc cagatgacac ccgccgacct ggccaaattc gagccgcaac
     5341 ggcgctacgc cactctcgtg gcgctggcca ccgagggcat ggccaccgtc accgacgaaa
     5401 tcatcgacct gcacgaccgc atcctgggta agctgtttaa cgctgccaag aataagcatc
     5461 agcagcagtt ccaggcgtca ggcaaggcca tcaacgccaa ggtacgtctg tacgggcgca
     5521 tcggtcaggc gctgatcgac gccaagcaat caggccgcga tgcgtttgcc gccatcgagg
     5581 ccgtcatgtc ctgggattcc tttgccgaga gcgtcaccga ggcgcagaag ctcgcgcaac
     5641 ccgatgactt cgatttcctg catcgcatcg gcgagagcta cgccaccctg cgccgctatg
     5701 caccggaatt ccttgccgtg ctcaagctgc gggccgcgcc cgccgccaaa aacgtgcttg
     5761 atgccattga ggtgctgcgc ggcatgaaca ccgacaacgc ccgcaagctg ccagccgatg
     5821 caccgaccgg cttcatcaag ccgcgctggc agaaactggt gatgaccgac gccggcatcg
     5881 accggcgcta ctacgaactg tgcgcgctgt ccgagttgaa gaactccctg cgctcgggcg
     5941 acatctgggt gcagggttca cgccagttca aggacttcga ggactacctg gtaccgcccg
     6001 agaagttcac cagcctcaag cagtccagcg aattgccgct ggccgtggcc accgactgcg
     6061 aacaatatct gcatgagcgg ctgacgctgc tggaagcaca acttgccacc gtcaaccgca
     6121 tggcggcagc caacgacctg ccggatgcca tcatcaccga gtcgggcttg aagatcacgc
     6181 cgctggatgc ggcggtgccc gacaccgcgc aggcgctgat agaccagaca gccatggtcc
     6241 tgccgcacgt caagatcacc gaactgctgc tcgaagtcga tgagtggacg ggcttcaccc
     6301 ggcacttcac gcacttgaaa tcgggcgatc tggccaagga caagaacctg ttgttgacca
     6361 cgatcctggc cgacgcgatc aacctgggcc tgaccaagat ggccgagtcc tgccccggca
     6421 cgacctacgc gaagctcgct tggctgcaag cctggcatac ccgcgacgaa acgtactcga
     6481 cagcgttggc tgaactggtc aacgctcagt ttcggcatcc ctttgccggg cactggggcg
     6541 atggcaccac atcatcatcg gacggacaga atttccgaac cgctagcaag gcaaagagca
     6601 cggggcacat caacccaaaa tatggcagca gcccaggacg gactttctac acccacatct
     6661 ccgaccaata cgcgccattc cacaccaagg tggtcaatgt cggcctgcgc gactcaacct
     6721 acgtgctcga cggcctgctg taccacgaat ccgacctgcg gatcgaggag cactacaccg
     6781 acacggcggg cttcaccgat cacgtcttcg ccctgatgca cctcttgggc ttccgcttcg
     6841 cgccgcgcat ccgcgacctg ggcgacacca agctctacat cccgaagggc gatgccgcct
     6901 atgacgcgct caagccgatg atcggcggca cgctcaacat caagcacgtc cgcgcccatt
     6961 gggacgaaat cctgcggctg gccacctcga tcaagcaggg cacggtgacg gcctcgctga
     7021 tgctcaggaa actcggcagc tacccgcgcc agaacggctt ggccgtcgcg ctgcgcgagt
     7081 tgggccgcat cgagcgcacg ctgttcatcc tcgactggct gcaaagcgtc gagctacgcc
     7141 gccgcgtgca tgccgggctg aacaagggcg aggcgcgcaa tgcgctggcc cgtgccgtgt
     7201 tcttcaaccg ccttggtgaa atccgtgacc gcagtttcga gcagcagcgc taccgggcca
     7261 gcggcctcaa cctggtgacg gcggccatcg tgctgtggaa cacggtctac ctggagcgtg
     7321 cggcgcatgc gttgcgcggc aatggtcatg ccgtcgatga ctcgctattg cagtacctgt
     7381 cgccactcgg ctgggagcac atcaacctga ccggtgatta cctatggcgc agcagcgcca
     7441 agatcggcgc ggggaagttc aggccgctac ggcctctgca accggcttag cgtgctttat
     7501 tttccgtttt ctgagacgac ccctattatg gcgaaggtat cattcaggaa gcgttgatga
     7561 aggattatct ctgtaaaaag ttattcaacc ggctttccgg taccctggtg atcagagcgc
     7621 gctgcggcaa taacattact ggcctggcat gttgcaatat tctttatccc tcgccccgat
     7681 acagcggtca gctgcatatt aaagagctgt atgtttctca gtgcgatcga aataagggca
     7741 cgggtaaagc gataatgcgc tttatagccc ggcttgcgct tgaacaggaa tgccttagcc
     7801 ttagctggaa cgctgaaaaa tccaaccccg gcgctaaccg tttttatcag gctctgggag
     7861 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     7921 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     7981 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
     8041 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaaccag
     8101 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     8161 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     8221 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     8281 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     8341 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     8401 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     8461 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     8521 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     8581 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     8641 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     8701 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     8761 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     8821 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     8881 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     8941 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     9001 cggcgcaaag tgcgtc
//
LOCUS       DAJVPT010000138         8862 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVPT010000138 DAJVPT010000000
VERSION     DAJVPT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            297..992
                     /gene="minC"
                     /locus_tag="OQM47_003472"
     CDS             297..992
                     /gene="minC"
                     /locus_tag="OQM47_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1481494.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1016..1828
                     /gene="minD"
                     /locus_tag="OQM47_003473"
     CDS             1016..1828
                     /gene="minD"
                     /locus_tag="OQM47_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1481495.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1832..2098
                     /gene="minE"
                     /locus_tag="OQM47_003474"
     CDS             1832..2098
                     /gene="minE"
                     /locus_tag="OQM47_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1481496.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2470..2814)
                     /locus_tag="OQM47_003475"
                     /pseudo
     CDS             complement(2470..2814)
                     /locus_tag="OQM47_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2849..2968)
                     /locus_tag="OQM47_003476"
     CDS             complement(2849..2968)
                     /locus_tag="OQM47_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1481497.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2929..3114)
                     /locus_tag="OQM47_003477"
     CDS             complement(2929..3114)
                     /locus_tag="OQM47_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481498.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3215..3388
                     /locus_tag="OQM47_003478"
     CDS             3215..3388
                     /locus_tag="OQM47_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481499.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3390..3734
                     /locus_tag="OQM47_003479"
     CDS             3390..3734
                     /locus_tag="OQM47_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1481500.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3738..4073
                     /locus_tag="OQM47_003480"
     CDS             3738..4073
                     /locus_tag="OQM47_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1481501.1"
                     /translation="MMMKKFALLAGLFVFAPMTWVQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4130..6451)
                     /locus_tag="OQM47_003481"
     CDS             complement(4130..6451)
                     /locus_tag="OQM47_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1481502.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6568..6777)
                     /locus_tag="OQM47_003482"
     CDS             complement(6568..6777)
                     /locus_tag="OQM47_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481503.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7528..>8862)
                     /locus_tag="OQM47_003483"
     CDS             complement(7528..>8862)
                     /locus_tag="OQM47_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1481504.1"
                     /translation="GKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLPGNRVL
                     LSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGV
                     RYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFG
                     ILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGTLRGVE
                     VLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEGFHIGI
                     NFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGF
                     VIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSIS
                     IVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2482 a         2061 c         1938 g         2381 t
ORIGIN      
        1 aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct gtcacgatta
       61 gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca atgggttgat
      121 tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata gacttttact
      181 ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat gccttatagt
      241 cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag gccaggatgt
      301 caaacactcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt catctgcatg
      361 aggcagaacc taaggttatc catcaggcgc tgaaagacaa aatcgctcag gcccccacat
      421 ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg gtaaactggt
      481 cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta agtggctgca
      541 aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg acggaaggaa
      601 aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat acaatgccgg
      661 tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt tatgctccac
      721 aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt gccgatggga
      781 acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg
      841 aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca ggtgaatact
      901 ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg cagttagtcg
      961 aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt taacaaggaa tttctatggc
     1021 acgcattatt gttgttactt cgggtaaagg gggtgttggt aagacaacct ccagcgcggc
     1081 catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg
     1141 cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg atttcgtcaa
     1201 cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc gtactgaaaa
     1261 tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc gtgaaggggt
     1321 cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt gtgactcccc
     1381 ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag ccattattac
     1441 caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca ttctggcgtc
     1501 aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc tgttaacgcg
     1561 ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg tgctggagat
     1621 cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa
     1681 ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct acgcagatac
     1741 cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag agaagaaagg
     1801 cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct ttctctcgcg
     1861 gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg ctgaacgccg
     1921 tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc ttgaggtcat
     1981 ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc aaaaagatgg
     2041 cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc tgaaataagc
     2101 ccgctgtaaa aacgcattta tcttcaaggc agagtcattt ctgccttgag tttttcatcc
     2161 ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc attgctattt
     2221 ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg tttatgcctg
     2281 gttgagtaaa taaccatgtt attagtgtgt atatactggg agatgataac cccgcaattt
     2341 cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg gctttttttg
     2401 ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc gcagtttaat
     2461 cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg actccacacc
     2521 ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga atgaggcttt
     2581 aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat taccgtcact
     2641 attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct tcaggcaggg
     2701 ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac ccagttcacg
     2761 atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat gatagtagcg
     2821 tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca ggggattcat
     2881 cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc atgatgaaat
     2941 gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa gtgtagttgg
     3001 ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac aatgaggccg
     3061 tcggaaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa
     3121 atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc
     3181 aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat ttaagattat
     3241 tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct
     3301 gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgtg
     3361 tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta
     3421 tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc acgaaaggtg
     3481 cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag
     3541 gtgcagctgt tggcgctggt gttagtgcag taacagaaaa aggccgcgac ggtaaaaatg
     3601 cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca
     3661 atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg
     3721 gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc ctgtttgttt
     3781 tcgcaccaat gacctgggtg caggactaca atattaaaaa cggtttgccg tcagaaacct
     3841 atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa
     3901 ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc
     3961 cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa
     4021 tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa taatatttca
     4081 tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat cagaacttat
     4141 atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc tgcacgccca
     4201 cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt ttaatttccc
     4261 ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt
     4321 tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc
     4381 tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg atgttgttat
     4441 ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga tcgacgccca
     4501 tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct tccagaccaa
     4561 aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca cctttcgcgc
     4621 tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag ctatattgcg
     4681 cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa gtggcataca
     4741 gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag
     4801 ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactatcgg
     4861 taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc gaggtcgttc
     4921 tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaagtc
     4981 ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc gcacggtcgt
     5041 tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt
     5101 cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg gagtttgttt
     5161 caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc gcttcggtat
     5221 tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt
     5281 ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg taagctccgc
     5341 tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca ttaataacga
     5401 ctttatccgt agctgaatta tcatcatcca attcagttct gagatgcaat acgccattat
     5461 taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt ccatctgccc
     5521 gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag tcagctttaa
     5581 tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca tctgttgaaa
     5641 tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga cctttaatat
     5701 tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc acgttactcg
     5761 tctcatcttt atggtagagg atttcatttg ctgaccgtag tgagctacca gataaaatat
     5821 ctaccgttgc ttcctgacca tttatggaag actccagcac aattccagac ccctgatgcg
     5881 cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta gcaatcgcgg
     5941 cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc tggcgggcag
     6001 cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg
     6061 cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg tacgcggcat
     6121 aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt gctatggcat
     6181 tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat acacacgcac
     6241 acgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa
     6301 taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag ttgactctta
     6361 ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt gagtaatccc
     6421 ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat tcacttacga
     6481 ttgcgctagc tgttaatgct gggcttagag gactattgtc agaaggatat aaaccgcccg
     6541 ttgttacaat gacgccattg ttaatggtta acttactacc ctctcctgca acggataccc
     6601 gtaacttcca ttacccgttg ttataactag cgcattatca atatctttta cttttccatc
     6661 ttttattgcc acagcatgtt tatattcatc gtgctgaata aactgttcag catatgcatt
     6721 acccaccgcc agagcaataa gtcccaaact aaacccgggg agttttttca gtttcataga
     6781 ttaactatcc attcattgga ttaacaaaca ttcctggtca aataacctat gagctcagat
     6841 taccaaaacg gagggtaaag acaaaaacaa aagcacaaaa aatttaaata gataaacagc
     6901 gacaatatat aaatgataaa taagtcggca ttatcatttt tcaccttatt ttcatgacat
     6961 tgatcacttt ggggtgacta ttttgtttaa caaataactc ttcgacatca aaattattaa
     7021 tagttgccat ggtaattatt tcggtgatat ataaaaacaa ctgtgataac accaaggttg
     7081 acaactattt gcgcaataat tcctttcctt caatgatcca ggcgttcact gccagcaacc
     7141 cattcagccg catactgaca gtgagaaact gaaagattat tgctgatccc tgtggctaac
     7201 gagtctttct cttaaatcga ttaaccagat tgatcgccag ccaaacaagg gcattaagca
     7261 aatttatcgt cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca
     7321 ggagggtgat cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat
     7381 tattactatt attcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca
     7441 aacaaaataa tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca
     7501 ccgcactatg gccttactga tcaattttca ctcaaccaca accttcacct tcggtttaga
     7561 aaggataatt ttgaccaggt cgatgtatgt aacaggttta tagaaataat aaccctgctg
     7621 aaatgtgata ttattttggt tcagatagcc aagttgttct ttcgtttcga caccttcagc
     7681 gacgatactg atcgaaagtt tacgcgccag atccaataca caatccagaa ttcgggttga
     7741 ttctgggtct gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg
     7801 caggtcatga agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac
     7861 aaaaccattt tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg
     7921 ctcacgctcg gtgacttcca gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa
     7981 atttaaacac tcgtcgacaa acgtcggcga aataatatgc gaagcgctaa aattaattcc
     8041 aatatgaaaa ccttccggca gtttactcgc gatagcgttc atctgtctgg caacctgatt
     8101 catcaggctt tgtgtaagcg gaacgattaa tccggatttt tcagcaagtg gaataaatgc
     8161 cgcgggtgaa atatatccac cgtgaggttg tttccagcgg gctaacacct caactccccg
     8221 caatgtccct tcccgaccat ttaccacagg ttggtaaaaa gggacgatct ccccattatt
     8281 tattgcccgt cgcaggatct cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag
     8341 gaaggcagcg gcgctggcga ttaacagaat aaatattaaa atcccaaagc ctttatcgat
     8401 aagtctgtag aagctaaaga gtggcggcgg gttaaagata atagtgaaat gatattttgt
     8461 ggagtgaacc ttctcggtat agggataatg tccattaagc gtcatcacat cagccgttgg
     8521 tccaataatg tcatccgcca cgcgtaatac ataccttacc cctttcaagg gtacattaag
     8581 cgccccgcga atatgttgat cacttatcgt aaccaaaatg cgcgtatctg caaattggct
     8641 ctgatagagc aggataggta atctattaac ggtgtcgatt gccggagcca acagtaaatt
     8701 actgtccggg acaacaggaa tacgagacag caggacccga ttcccaggca gggatgtaca
     8761 ccagactatt ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc
     8821 agtgccaagc tgatattgct cctcgaggtc gcattccttc cc
//
LOCUS       DAJVPT010000139         8799 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVPT010000139 DAJVPT010000000
VERSION     DAJVPT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..640)
                     /locus_tag="OQM47_003484"
     CDS             complement(<1..640)
                     /locus_tag="OQM47_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1481505.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLT"
     gene            700..978
                     /locus_tag="OQM47_003485"
     CDS             700..978
                     /locus_tag="OQM47_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481506.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(979..1506)
                     /gene="gadE"
                     /locus_tag="OQM47_003486"
     CDS             complement(979..1506)
                     /gene="gadE"
                     /locus_tag="OQM47_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1481507.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2305..2877)
                     /gene="hdeD"
                     /locus_tag="OQM47_003487"
     CDS             complement(2305..2877)
                     /gene="hdeD"
                     /locus_tag="OQM47_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1481508.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3132..3464
                     /gene="hdeA"
                     /locus_tag="OQM47_003488"
     CDS             3132..3464
                     /gene="hdeA"
                     /locus_tag="OQM47_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1481509.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3580..3906
                     /gene="hdeB"
                     /locus_tag="OQM47_003489"
     CDS             3580..3906
                     /gene="hdeB"
                     /locus_tag="OQM47_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1481510.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            3970..4616
                     /locus_tag="OQM47_003490"
                     /pseudo
     CDS             3970..4616
                     /locus_tag="OQM47_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4658..5188)
                     /locus_tag="OQM47_003491"
     CDS             complement(4658..5188)
                     /locus_tag="OQM47_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1481511.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5344..5910)
                     /gene="slp"
                     /locus_tag="OQM47_003492"
     CDS             complement(5344..5910)
                     /gene="slp"
                     /locus_tag="OQM47_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1481512.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6157..7380)
                     /locus_tag="OQM47_003493"
     CDS             complement(6157..7380)
                     /locus_tag="OQM47_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481513.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(7961..>8799)
                     /locus_tag="OQM47_003494"
     CDS             complement(7961..>8799)
                     /locus_tag="OQM47_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1481514.1"
                     /translation="GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSP
                     LLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCA
                     PAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDV
                     LPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASV
                     LMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQL
                     LF"
BASE COUNT         2593 a         1716 c         1835 g         2655 t
ORIGIN      
        1 gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat
       61 tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg
      121 aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg
      181 tgacattggc ttctgcttca ttcagctggg tgcgcgcggt gtcgtaatcc tgacggctaa
      241 cgtagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg
      301 tagagagcgc tttcgccagc gagcctttgg cggagtttag ctcggcctgt aaaggtgcag
      361 gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt
      421 taatgataat accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac
      481 cgggcaattc gctcaacaca ttgaccgaac caggggagag tgtgacgaca ccgacctcag
      541 gcgtcatggc ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc
      601 agaataacaa cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt
      661 gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc
      721 ggcatagtgg ggttgtcagc cagtaaggtg ggatacaggc acagtgatcg acatggtgag
      781 gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg
      841 aagggttcta ctggtggata cacataccag gggaatcaag tcgttttagc ctctccttta
      901 gtaatcaccg ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt
      961 cagttgctta tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat
     1021 acgtcttagt ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga
     1081 aatattgagc gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat
     1141 tttgaactga ttatgtgaaa aacataattg tgaattctta tggggcaagt gtttaccata
     1201 agtaatctca agaagatttt tgatactttc tttgcggcta acaaaataaa taggcgcttt
     1261 agcttttagt ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt
     1321 ctccagaaat ttaatcgctt cttcatcaag gatatgattg tgataggtat caatgataac
     1381 cttgaagggt ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc
     1441 gtgattatct ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa
     1501 aatcataact tgctccttag ccgttatcgt ttcgaacatg tcatcctttt cattatttac
     1561 atccttgtcc gaatcgttgt tcaatatagt aaacgcctat aagaactttg cccttcgtga
     1621 actaatacgt ctattaacac taatcaatac ttcagattta tccatcacgc actccatata
     1681 ttgatcctaa gtaagattcc tggttgttat cagcttgtaa atagtaaaca aaaagttaac
     1741 gtttgcggat tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat
     1801 attactaaag ttaaaatgta atccgattta aatatcgagt ctccttgttt cgacttaagc
     1861 tggcaattgg attgccagct ttcttttaac gcattgaaat tacctgagta aataacgcat
     1921 tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca atgtaatcaa
     1981 cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc atcaacgctt
     2041 acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg actacggaaa
     2101 atatcagcca tgataatgtt tgaaatggct aatttgccat ggagtgaaaa aaattagatg
     2161 aaattcagta ggttgaaata atcactagca ggtaattatt tcaatgatag tgcgcaattg
     2221 atctacaaca ctgcgtagcg gagagagtat taatcggatc atagtcacat caagtgacta
     2281 tgatccgggt gacaaccggg gtaattattg ctgcttaacg aacaaactgg cgaagctgaa
     2341 caggctggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca gcgttacaga
     2401 caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac
     2461 gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac
     2521 ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctccgg
     2581 tgcacggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa
     2641 taccggccag aaattatgac tgcggttgct gaataacccg acaataagcg caatacccga
     2701 gcagattaat aatgcaccca ctactgtgct taaaatatcg ccagagacga acgggaaact
     2761 gatacacagc aacccgacga taaacagcag cacggcaata aactggattg ctctgcgatg
     2821 ctttttcagc atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga
     2881 accaccctat aaaattaaga agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct
     2941 aagaatgcag tcgatttaat aaaaatttcc taattgcagt atctgatgca tctgtaactc
     3001 attgtattga aataaaaata tctgattttg atattttcca tcaacatgac atatacagaa
     3061 aaccaggtta taacctcagt gtcgaaattg attcgtgacg gctctttcac tttatagttg
     3121 aggatattac gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt cttctgccag
     3181 ttgtgagcaa tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg gtcaactcat
     3241 ggacctgtga agatttcctg gctgtggacg aatccttcca gccaactgca gttggttttg
     3301 ctgaagcgct gaacaacaaa gataaaccag aagatgcggt tttagatgtt cagggtattg
     3361 caaccgtaac cccagctatc gttcaggctt gtactcagga taaacaagcc aactttaaag
     3421 ataaagttaa aggcgaatgg gacaaaatta agaaagatat gtaattccgg gaatgcgtta
     3481 catcgtactt ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc agctattcct
     3541 cctgttcata tataatctct atattgaatg ggttacaaaa tgaatatttc atctctccgt
     3601 aaagcgttta tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg
     3661 ttggcagcca atgaatctgc taaagatatg acctgccagg aatttattga tctgaatcca
     3721 aaagcaatga ccccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc
     3781 gataccgtta ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgt
     3841 aagaaaaacc cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg
     3901 aattaatgag gtacaagtaa aaaggagtag caagttgagc catcttgctg ctcctttttg
     3961 catttttata tgacagcaga atttattata cgtcttatac tcgcggcaat tgcctgtggc
     4021 gctattggca tggaaaggca aatgcgcggc aaaggagcag ggttacgcac acatgtatta
     4081 attggcatgg gaagcgccct gtttatgatt gtttcgaaat atggttttgc tgacgtgctg
     4141 tctttagatc acgtcggact cgaccccagc cgtattgctg ctcaggtggt gacgggcgtc
     4201 gggtttatcg gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tctgacgacg
     4261 gcagcggata tctgggtgac cgccgcatag gtatggttat tggcagcggt atgtacgaac
     4321 tgggtattta tggttcagtg atgaccttgt tggtgctgga agtcttccat caattaacct
     4381 tccgcctgat gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat
     4441 caatgctcga ctggtttaag cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa
     4501 atgaagatca tgaagtggtc gccattgata tccagcttca tgccacaacg ttgatagaag
     4561 acctgttgcg attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg
     4621 tgtggcagcg tagccagact caccgtaagc ctgaaattca caccagataa tcaatatgct
     4681 gatggcgtac cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat
     4741 ggcagtagaa cgtttttagc gatattttca gtttctccag aatatcttca gttgtcattt
     4801 gattcgcaat gcagctcaga acctgatgtt gcttgcgcga aagtgacaca ttcgcttcag
     4861 gcacagagca gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg
     4921 gaacaaaggt caccggtact ttccccagac agcgtttaat attaaagggg gagaaaacaa
     4981 tgacatggac aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat
     5041 tcattaatgt atcaataatg acgaaggcat tctgatgcaa cattacgtcg atctcttctt
     5101 cgttctgtat atgaacgaca ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca
     5161 tcgtatccct ggtaattata agaaacataa ccgactcctg gtgcggacat taactttata
     5221 atcagtttgc caaaggtaat taacaggaca gggcttataa taataatagt tagcttactg
     5281 tatattaatt ttatgtaata catgatgcag atgctgagaa agcatctgca tctttcggtg
     5341 gtgttatttg accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc
     5401 ccagcccggt tccggccaga atgcaccata gccgtaatcc cacaggttat aggtggtatt
     5461 aaccacttct ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata
     5521 cgggacttta ttgataaagc ctggttgttc accctgaatg gtaccgagga tggtaacaaa
     5581 gtgattacga tagttcactg gatcaaggaa gccgctttgt ctggcgagca gtcggccctg
     5641 atagttggct tcaatatcag gcttcgcata gctatccaac ggtaatacag cgatttctaa
     5701 caacgtatcc gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg
     5761 accaacatat aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg
     5821 gttattgcct ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct
     5881 gaggatgagt gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac
     5941 cataatagat atttctttat agttttcatc tgattctgag gtgatatgcc tgatagtaac
     6001 gtaaacctgt ctgatgttta tatcccttct gtttagttca attccgttat tttggggcgt
     6061 ggtatgagta tccatcgccc aataatataa ccctttgttt taaaattaca taaaatctat
     6121 ttttattcaa tgattataaa gtgctaataa tcaatattat ggtattatat tctgataatg
     6181 atttgataag cgaattcttt tgccagatgg tatttctatt tccaccccat aatttatatc
     6241 tttttcatta ggcagatctc tctttaaata aaaggggtat gaaatctttt cgggttgaag
     6301 tgtctcgtaa tgaaattcta tgaattcatt tttagacatt gttgcccatt tttgaatttt
     6361 attgtataga gagggtgttg taaactcttg aagttgttta taacatattt ttttgaactc
     6421 ttgcttatat tttaatatgt catcaaatga aagattcact acctttgcct ctaagaaatc
     6481 aattattctc gaagcaattt ccgctttcgc gcgtaaagag ttgtcattaa tagttaacaa
     6541 tgtagaaaac aaaccattga tttgtttgtc aagatgttgt tcatatttat gtagtttggg
     6601 gatgggattt tccttgtgtg aaaaaatagt gttaaataaa tttgcgtcag tgaaatttat
     6661 attaagtaga tcttcaaaat aaaattttga taaggttagg actagcttgg acatatcttg
     6721 ccctgtcatg ttgaaatcaa taaagtcgca tccagcaagg gcttccatcc tattttcatt
     6781 tttactgtgc tcaatataca gcttcgccat ttttaagtac aagctttgaa tgtctcttat
     6841 atcagttggt aagtcagctc gttgcccagc aaaattttta ttatcggaag aaacggtttg
     6901 gacactgaat ctttcgggta ttccttcttc tacgagaatt ttagttatga ttggattgtt
     6961 gggttctgga ttaacgacaa caatgatttg acatacagaa tccttatcct tttcgtatct
     7021 aatgttatga atctgattaa gtattgattg tgtctgttgc gtaagtggtg tcgaacttat
     7081 cgtttcaatg ttattaataa gacttacaag ttgaaccaca acttttgagt agtctgcatt
     7141 tgtatatgtg gtgttcgtgt ggcatgcatt aatttgttgg agttgttgtt gcgctaaatc
     7201 aaattgttca gggttgtatc cttctccaga gaatgtggaa ttaatgggtt gttgttcttc
     7261 ccaattatca caaggccact tcggtgtgtc tggcgtttta acattatcgg tatttgttgt
     7321 gcagttataa atatcgccgt gatatgtatt tattatacct ggggtaaagt caattgacat
     7381 aacattcctt attcatcatc tgttttatgc taatgttttt aaattggata attagaacag
     7441 gaatggattt tcttctacac acaataaata taatgcgtta tatcttcctg atataattac
     7501 ctttaaattc cctttcattt ccacccggta atatttgtgt ataaaaaaag ggcagtgata
     7561 gtatcgtaaa ggaaaacgtt aacaactatc aaaaaacgct cattttaaaa agttattatg
     7621 ttctccgata gtgtgtgaag taatattttt gtgatgtttt agatgtgtaa taatgaacca
     7681 ggttaaagtg tatttaaaaa tgtgttgtat tagcgactga aatctgcctt ttaaaaagga
     7741 ggagggtact gagacaaaga gtcattgctt ctccgataaa atgtcagaga agcatgatta
     7801 cgtggttatt gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt
     7861 taaatcattt gtgattttta aggattatcc tgtctatgat tttctactta gaaggatatg
     7921 gcactaacta tgcatcttat agtttactgc tggcatctat ttaaaatagc aattgagtca
     7981 aatagcccat caagatagcc atacctaata taaccgtcag gaatgcagct atcatcggca
     8041 ttctgaacat tgatttaagt aatatcactt ccgtcaggct tgcgccggca ctgccgatga
     8101 ttaatgccat taatgctccc agacccattc cttttgtcat cagaacagat gccagaggga
     8161 taaccgcctc agcccggata tacagcggaa taccgacaac ggcgcttaat gggatggcga
     8221 agggattatc tgctcctgca tgagcggcaa tccacgctga gggaataaag ccataaataa
     8281 aagatcctat taatactcct aacaaaagat atggcagtac atctttaaac tgttgcaacg
     8341 catctttggt ggctagtttt atgggattaa tacttactac agctttggta ctgcaacagg
     8401 taactacggg gttctcaatg gctttagccg ctggactaca acaactcact tttatgggcg
     8461 tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca atttgctgat gagcttttac
     8521 tggcaatgat atgacgttca aaccccagag aatccaggat aatactggca agaacggaga
     8581 cgccggctgc gataatcgcg tacaacaagg tgactttcca accaaaggtc atccacatta
     8641 gtccgacgat aatgggatta agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa
     8701 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
     8761 gggtaacggc tcccaacaga gcagccagga gatagcctc
//
LOCUS       DAJVPT010000140         8763 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVPT010000140 DAJVPT010000000
VERSION     DAJVPT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            7..438
                     /locus_tag="OQM47_003495"
     CDS             7..438
                     /locus_tag="OQM47_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1481515.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OQM47_003496"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OQM47_003496"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1481516.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OQM47_003497"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OQM47_003497"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1481517.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OQM47_003498"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OQM47_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1481518.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OQM47_003499"
     CDS             complement(4440..5147)
                     /locus_tag="OQM47_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1481519.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OQM47_003500"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OQM47_003500"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1481520.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OQM47_003501"
     CDS             complement(6005..6208)
                     /locus_tag="OQM47_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1481521.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7875
                     /locus_tag="OQM47_003502"
                     /pseudo
     CDS             6397..7875
                     /locus_tag="OQM47_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8763
                     /locus_tag="OQM47_003503"
     CDS             7872..>8763
                     /locus_tag="OQM47_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1481522.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2033 a         2389 c         2252 g         2089 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catggcgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacctg
     8761 aac
//
LOCUS       DAJVPT010000141         8671 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVPT010000141 DAJVPT010000000
VERSION     DAJVPT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OQM47_003504"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OQM47_003504"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1481523.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OQM47_003505"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OQM47_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1481524.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OQM47_003506"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OQM47_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1481525.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OQM47_003507"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OQM47_003507"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1481526.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OQM47_003508"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OQM47_003508"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1481527.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OQM47_003509"
     CDS             complement(7826..8137)
                     /locus_tag="OQM47_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1481528.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OQM47_003510"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OQM47_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1481529.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
BASE COUNT         2171 a         2245 c         2055 g         2200 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tgattttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgttgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctaacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa catcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgcaccaaa ataggcatca ccaatatttc gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgcaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta actataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttccccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg aaggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg t
//
LOCUS       DAJVPT010000142         8637 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVPT010000142 DAJVPT010000000
VERSION     DAJVPT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(78..803)
                     /gene="fimC"
                     /locus_tag="OQM47_003511"
     CDS             complement(78..803)
                     /gene="fimC"
                     /locus_tag="OQM47_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1481530.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(840..1379)
                     /locus_tag="OQM47_003512"
     CDS             complement(840..1379)
                     /locus_tag="OQM47_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1481531.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1444..1992)
                     /gene="fimA"
                     /locus_tag="OQM47_003513"
     CDS             complement(1444..1992)
                     /gene="fimA"
                     /locus_tag="OQM47_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1481532.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(2473..3069)
                     /gene="fimE"
                     /locus_tag="OQM47_003514"
     CDS             complement(2473..3069)
                     /gene="fimE"
                     /locus_tag="OQM47_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1481533.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(3547..4149)
                     /gene="fimB"
                     /locus_tag="OQM47_003515"
                     /pseudo
     CDS             complement(3547..4149)
                     /gene="fimB"
                     /locus_tag="OQM47_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            5603..6319
                     /gene="nanC"
                     /locus_tag="OQM47_003516"
     CDS             5603..6319
                     /gene="nanC"
                     /locus_tag="OQM47_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1481534.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6339..7445
                     /gene="nanM"
                     /locus_tag="OQM47_003517"
     CDS             6339..7445
                     /gene="nanM"
                     /locus_tag="OQM47_003517"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1481535.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            7510..8490
                     /locus_tag="OQM47_003518"
     CDS             7510..8490
                     /locus_tag="OQM47_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1481536.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2539 a         1858 c         1800 g         2440 t
ORIGIN      
        1 agatatgaca ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc
       61 gaaaaattcc ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa
      121 tcatttattg ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt
      181 tcgcccattg gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt
      241 accgtcaggt aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat
      301 cttaattttt ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt
      361 ttaatgcggc tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt
      421 gacggaatcg ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta
      481 tttgttgcat caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga
      541 ggcgtcacga taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt
      601 aaataggtac tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc
      661 ggataaatta cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc
      721 attgccatga acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt
      781 acattgacgt ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat
      841 tactgatagg ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc
      901 ccggtagcac gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc
      961 cagtttgctg gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca
     1021 ccaatattgg aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca
     1081 tccgcgacac cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt
     1141 aaatgaataa caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg
     1201 ctgatttgcc ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc
     1261 gcaataatga cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt
     1321 ccggccagag caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc
     1381 gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt
     1441 aggttattga tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc
     1501 gccgattgca taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt
     1561 ttgtgcacta aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ctaggatctg
     1621 cacgccaacg tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg
     1681 cgtagcatca attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc
     1741 gcaatcattc agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag
     1801 gctagcggta cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc
     1861 gcaagcggcg ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt
     1921 atcggccaga gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt
     1981 tttaattttc atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt
     2041 tctgtacact ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa
     2101 agatgaaaca tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc
     2161 agtttatttg ttggcttaat attctattat tatctttatt tatagatttt tatattgcat
     2221 gaggtggttt ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt
     2281 acatatagcg gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag
     2341 ttagatgctt tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt
     2401 tatgatatgg acagtttggc cccaattgtc ttgtgtttat ttgtttttta actttattat
     2461 caataagtta aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc
     2521 ccataatccg gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt
     2581 tcgatgcccg agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata
     2641 accgcaagca tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc
     2701 ggcatcgcga ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat
     2761 aaatatagcg tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc
     2821 cacggcttca cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg
     2881 gcgaatattt attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc
     2941 actaatacgc atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt
     3001 tgccccgtaa caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg
     3061 tttactcaca atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac
     3121 ctcaatcggt caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc
     3181 tatcagtcaa ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct
     3241 attttcattg atttgattca atataacaat agattacaga ggattaactt ccagttactt
     3301 aaaaaataaa gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat
     3361 gattttgttt atttattttg aagaacataa atcattagat tttgtatggg tatggttaac
     3421 ttaatgttta ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt
     3481 aatcattttt gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa
     3541 ctatctctat aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa
     3601 acgccctgca ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata
     3661 atcctagata agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg
     3721 taacatgtgc ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat
     3781 atggtaaaac tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct
     3841 ctcagcatgc ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt
     3901 gttcaatagc gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt
     3961 tgctttaaga tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg
     4021 aataaaacaa agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc
     4081 tttaaggagt gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt
     4141 attcctcatc gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata
     4201 atccaacatt tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt
     4261 aagtggacca gttcttctga gcgaacttaa atggagtagc gatagttact tataataact
     4321 ttgccataca aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat
     4381 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
     4441 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac
     4501 tagccgtctc aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat
     4561 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
     4621 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat
     4681 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca
     4741 ctacgactta agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
     4801 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
     4861 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
     4921 atggcgagta aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg
     4981 tttagattct aaataaatta tttataaaac gtatagttaa aagaaaatct ttagtttttg
     5041 aagaccaccg caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga
     5101 catattttgt gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
     5161 ctggtataac aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc
     5221 tatggcaaac atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa
     5281 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg
     5341 agtaagtgat ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact
     5401 caggtattta atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga
     5461 gaccttatac aaaaaatata aaatacagtt atccgttgcc aaagacacta taagcctggc
     5521 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     5581 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     5641 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     5701 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     5761 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     5821 cgcactcaat gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac
     5881 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
     5941 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     6001 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     6061 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     6121 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
     6181 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     6241 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     6301 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
     6361 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     6421 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     6481 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     6541 ctgcattccc tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt
     6601 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     6661 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca
     6721 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     6781 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     6841 tagataaaat caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt
     6901 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     6961 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     7021 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     7081 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     7141 ggataagcaa tgattctctt atatttgctg gaggggccgg attcaaaggt tcacgagaaa
     7201 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     7261 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     7321 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     7381 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     7441 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     7501 gaaaattata tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt
     7561 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     7621 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     7681 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     7741 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     7801 catattgcac ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg
     7861 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     7921 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     7981 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     8041 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     8101 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     8161 actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     8221 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     8281 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     8341 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     8401 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     8461 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     8521 gttcgttctc agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg
     8581 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacg
//
LOCUS       DAJVPT010000143         8436 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVPT010000143 DAJVPT010000000
VERSION     DAJVPT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..306
                     /locus_tag="OQM47_003519"
     CDS             <1..306
                     /locus_tag="OQM47_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1481537.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2188
                     /locus_tag="OQM47_003520"
     CDS             278..2188
                     /locus_tag="OQM47_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1481538.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2189..2392
                     /locus_tag="OQM47_003521"
     CDS             2189..2392
                     /locus_tag="OQM47_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481539.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="OQM47_003522"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="OQM47_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="OQM47_003523"
     CDS             3972..5477
                     /locus_tag="OQM47_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1481540.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="OQM47_003524"
     CDS             5514..5861
                     /locus_tag="OQM47_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1481541.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="OQM47_003525"
     CDS             5919..6947
                     /locus_tag="OQM47_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1481542.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="OQM47_003526"
     CDS             6999..7385
                     /locus_tag="OQM47_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1481543.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="OQM47_003527"
     CDS             7397..7774
                     /locus_tag="OQM47_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481544.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..8345
                     /locus_tag="OQM47_003528"
     CDS             7761..8345
                     /locus_tag="OQM47_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481545.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8342..>8436
                     /locus_tag="OQM47_003529"
     CDS             8342..>8436
                     /locus_tag="OQM47_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481546.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPA"
BASE COUNT         1960 a         1990 c         2599 g         1887 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc agtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgtgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca ccagggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtattttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgcagttgc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tagctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagttctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggctgcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacggcccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccgatgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc atcattgctg
     8281 cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta catctgagta
     8341 aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg aacagtgtgg
     8401 agacagcgcc acgttttttg acgggcttcc ggcatt
//
LOCUS       DAJVPT010000144         8333 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVPT010000144 DAJVPT010000000
VERSION     DAJVPT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..158
                     /locus_tag="OQM47_003530"
     CDS             <1..158
                     /locus_tag="OQM47_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481547.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OQM47_003531"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OQM47_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1481548.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OQM47_003532"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OQM47_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1481549.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OQM47_003533"
     CDS             874..1245
                     /locus_tag="OQM47_003533"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1481550.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OQM47_003534"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OQM47_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OQM47_003535"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OQM47_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1481551.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OQM47_003536"
     CDS             6948..7508
                     /locus_tag="OQM47_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1481552.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OQM47_003537"
     CDS             7639..7851
                     /locus_tag="OQM47_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1481553.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8333
                     /locus_tag="OQM47_003538"
     CDS             8095..>8333
                     /locus_tag="OQM47_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1481554.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2085 c         2671 g         1664 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgtgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac ata
//
LOCUS       DAJVPT010000145         8157 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVPT010000145 DAJVPT010000000
VERSION     DAJVPT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(52..2964)
                     /locus_tag="OQM47_003539"
     CDS             complement(52..2964)
                     /locus_tag="OQM47_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481555.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            2967..3083
                     /locus_tag="OQM47_003540"
     CDS             2967..3083
                     /locus_tag="OQM47_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1481556.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3447..4289
                     /locus_tag="OQM47_003541"
     CDS             3447..4289
                     /locus_tag="OQM47_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1481557.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4292..5380
                     /gene="capU"
                     /locus_tag="OQM47_003542"
     CDS             4292..5380
                     /gene="capU"
                     /locus_tag="OQM47_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1481558.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5385..6335
                     /gene="virK"
                     /locus_tag="OQM47_003543"
     CDS             5385..6335
                     /gene="virK"
                     /locus_tag="OQM47_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1481559.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6400..7344
                     /gene="lpxM"
                     /locus_tag="OQM47_003544"
                     /gene_synonym="msbB"
     CDS             6400..7344
                     /gene="lpxM"
                     /locus_tag="OQM47_003544"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1481560.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7498..>8157)
                     /locus_tag="OQM47_003545"
     CDS             complement(7498..>8157)
                     /locus_tag="OQM47_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1481561.1"
                     /translation="CQLNAGTVRRWLPSVGIVWRRAAPTLRIRDPHKDEKMAAIHKAL
                     DECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKV
                     SYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGI
                     YQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAK
                     V"
BASE COUNT         2163 a         1840 c         1910 g         2244 t
ORIGIN      
        1 cacgtactta cgtacagatg aagttcggtt acatcctgtc agcacggaac ctcacgcttt
       61 ttgagcggca cgacggatcc cccccaccca gtatccgaca gacttttcac tgcgttcgta
      121 aaaacccttc ttccctccca gcatatccac taattccggt gacatatcct gcagataagt
      181 ctgcaaatca tgccccagat cttccggtga ctgcaactca taaaccgcgc gccacacatc
      241 acccttcctt attttaagcc ccgggttaag aatatactgt ccgtggttca gcctcagaaa
      301 aagctttttt ccataaggat ttttgctgtt aacttcatgt tgagagagct ggctgctgat
      361 ataactcctt cgtttgcggc gttccggcag caacgcttca ggaagatgaa ccacacactc
      421 tgccagtcgt gccgcactga aggcttccga aaaaaacaga gcacttccca gatgcgtgtg
      481 gaacagcgcc tgcatcagga tcaccagaaa cattgccatc tgccggttgt ccagtttgat
      541 gaggcgtcct tccacctgca gtgacagact ttcaggtttc agcagggtat acaactgcgc
      601 acttttctgt tgcgcataac ggggcgtact gaccgccgct gacagaactt gatgataagc
      661 cgccagcccg tcattaccgg tcagcaaggg atctgcacca cgctccagca gtaactgaac
      721 cagtgcaata ttacctgccc gggctgctga catcagcgga gtaagattaa acggcgaacg
      781 gtgatcaaca ccataacgtt caatatcccg caacacggca accgggttat tcattgagta
      841 tggccggaaa tacttcagct ccagtaactg cagagtcttc gttctgggct gccttgtggg
      901 cttaaaatct tcccggtaca gtgcattata cagcgggaac agggaataaa tcagactgta
      961 ctccagcgcc tgcagttgta tggttttatc ggctgtacct gcgaggatat gctgtttcca
     1021 ctgtcgtgca tcctctgcgg tgataatctg ccatggcatc tcagatgtct gcaggatccg
     1081 gctacggatg gtgtccgcct gctcctgctt cccctgtttt tcaagcagat gtgcggtttt
     1141 ctgccactca tcacggctgg aaatctcttc ctgcagattc agaggctctt cctgatgcgt
     1201 gagcgcaagc agactccaca gaggatgctc cacctgctgt tcaacaagat acacattgtg
     1261 tttagcacgg gtgagcgcaa catacagcgc gttggtaaaa aatttataga tttccgctga
     1321 acgatcctgc ctgtcccgcg gacgggaata acgggcctcg ccctcaagat cagccggtgt
     1381 aagaccctcg caaatatcat caaatgcctg ccgggcagcg cttactatat tgtaaaggat
     1441 caccgtttcg tattccagcc ccttaacctc ctgaaccgaa aagaccagcg gcgtactgaa
     1501 ccagcatcgc gcctgtgctt tctgttccgg atgcatcaca atgaccgcta cacgattcga
     1561 aagtgaggtt ttggcattca gttcctgctt tgtctcttcc ctatcgtcca gcaaccggat
     1621 ggtcccttct gcctgaccgc agcttctgac aaaatggtga ctttcccgat caatagcact
     1681 aaagcggacc tgtttgagtc tcagcaaccg gttagccagt gcagtgacgt ggtgcccgtt
     1741 acggtaattc gcctgcagga tattaacggt tgtgtcgttt ccctgttgct gccggaaaaa
     1801 gagacttttg agcgaactcc atgagaaaaa gctcggatga actatctgat tggcatcacc
     1861 acagagtaaa aactgaccgg gatgacgtag cgttttcagt accagttgca gttgaacagg
     1921 cgtcatatcc tgaacttcat caacaaaaat gacatcataa cggggagttg ccaacgaaag
     1981 atactgatga ctgagcagat tactgtcata ctgatgcgac tgtttcagcc atgcgatata
     2041 acgttcaaac aggacataga ccgtcgggcg gtcctccatg ccatagagcg actgccgtat
     2101 ccccagtgac agatacgcct cccgggacag cggcccattc ccggaggcaa ccgcaccgat
     2161 cacgcccctg aactcctcat acagggtatg ggcagccccc agtgcggcga tagccctgtt
     2221 gcggggcagc cagtcgctga acgcactgaa agtcacctcc ctgccttccg gaatgcgcag
     2281 agtttccaga aattccgtca gcgaaagaaa gtcaatattc tgtacctcac tcccttcccc
     2341 tgaagcgtca tagagagtac gcgctttttc caccagaaat gaggaaaggc tgagatacag
     2401 tatatcacca gcagcctgct tcattttttc cagtaccagc gccgtcttac cacttcccgc
     2461 cgagccaaca atcaccagcg gtagcgggtg ttcgtacagt gcctgctgat catcatcaaa
     2521 ggacagcatt ttatccaggc ggtggaaacg cccatgagac ggattgatat acgtcagcgc
     2581 ttcagtcgcg atatcaacag gatccggcac tggttgttgt tgtaagcgcc cttcatcaat
     2641 caccacgttt ctgcgaagaa aacgtgaagt gttataggca tgattgcgaa tatattccag
     2701 aacaaggcag atagtttcac cgtggtagcg atacagcgaa aacagtaatc ggtcccggat
     2761 actgagccgg gcgcggtaaa gattggtgtc aattttacga acatcagcct gaacaaagtt
     2821 atcggtggca atagcctgtg caaatttttt ataacccggg atcgtgtcag gattcagagt
     2881 atgataaaga cagattttca tggctgccgg tttccgtcgt gcaaaaaaca tgctgatttt
     2941 acgaaggaat gccttactga acaaccttgc ccggaatacc ccccgaaatc agatggaaca
     3001 atcagaaaaa tgctctgcag ttatggaata aagaaggtgg ggtggtacgc cgggagggcg
     3061 atccgctgga aatggcgcag tagctgcagg cctgtcatga tgcaggaata gaagttcgtg
     3121 tacaggttaa tgaaagcgtc accccgtaaa taaactctgc gctgaaattc ctttttttca
     3181 ttggagtacc tgtgttgttc tgaggtgaac atatcacctc tgttcaggta gccaaattca
     3241 gtgtgccacc tcaaggttct tagtgagtcg tttttaaaag atatgataat atatcattgt
     3301 ttatgaattg ttgatgttta atttagtaaa ttatatcaaa tatgccatgg gtttgctatg
     3361 ttattcatcc agtatctgga aataaaatgc accagcttta ttgggttttc cttttgtctc
     3421 tgcttgctag gtgccagaaa ctttaaatgt taaacgaagg gggcattttg ttcaaagcca
     3481 atcatgttcc ggtattaatg tatcatcatg tcagtcattg ccccggactg gttacacttt
     3541 ctcccgtgac attccggaag caaataaaat ggttggctga aaacaactgg aaaacactgt
     3601 catcagatga actggagttt ttttatcggg gcggaaaact cccccggaaa agcgttatgt
     3661 tgacatttga tgatggatac cttgacaact ggtttcaggt atatccttta ttaaaagagt
     3721 ttaatctcaa agcacatatt tttttaatta ccggttttat tgggaatggt ccggttcgtc
     3781 actctcctgg aaaggaatat tctcatcgcg attgcgaaca tcaaatagcg acagggaatg
     3841 ctgataatgt tatgcttcgc tggtctgaag taaacgaaat gttgcaatct ggtctggtcg
     3901 aatttcatgt tcatacgcat actcatacac gctgggataa aaagttcagt tcacgagaag
     3961 aacagtgtaa acatcttcgg caggacttgt tgagcgggag ggaatatttg aaagaaatga
     4021 caggaaaatg cagtaaacat ttgtgttggc cggaaggtta ttataacaag gattatattc
     4081 aggtcgctga agaacttgga ttctattatt tatatacaac agaaagaaga atgaatgctc
     4141 ctgctaaagg aactacacgg ataggaagaa taagcaccaa agaacgggaa agttgcgcat
     4201 ggctgaaaag acgacttttt tattatacaa ccccgttttt ttcatcgttg ctggcctttc
     4261 ataaggggcc aagattaccg gatgattaat aatgaatata ctatttacgg aatcatcacc
     4321 gaatataggt ggtcaggaat tacaggctgt tgctcaaatg aaggccctga agaaaatggg
     4381 gcattcagtt ctgcttgtct gcagggaaaa cagcaaaatt gcttttgaag ccagtaaatt
     4441 ggggattgat atcacattcg cgttatttcg aaacagtctt cacatcccta ctgcatggag
     4501 attactcgga atagttcatg gttttcagcc caatgcaatc gtttgtcaca gtgggcatga
     4561 tagcaatatt gttggtttag tacggttatt cactaggaaa catccattca gaattatcag
     4621 gcaaaagaca tatttgacac gaaaaacaaa agttttttca ataaatcatt tttgcgatga
     4681 ggtgattgtt cccggaacaa gtatgaagac acatctggag caggaaggat gtcgaacccg
     4741 ggttactgtt gtgcctccag gctttgattt ccagaaatta tacgttgatt cccgaaacag
     4801 tttgcctcca aatgttcttt cttggctggc gtcccgaagg ggatgccccg ttattgctca
     4861 ggtaggaatg ttgcgcccgg aaaaagggca cgaatttatg ttgaatttac tgttccattt
     4921 aaaaatgaat ggacgacagt tctgttggtt gattgtgggg tctggttcgc ctgaactgcg
     4981 ggagcattta cagtatcaga ttgacagtat gggcatgcat gatgatgttt ttattgctga
     5041 caatgttttt cctgccgccc ccgtatatcg ggttgccagt ctggtggttc tgccttcaga
     5101 aaacgaatcg tttggtatgg tactggcaga agcatcggca ttttctgtgc ctgtactggc
     5161 cagtcagatt ggtggaatcc ctgatgttat tcagaataac cagaccggga cattgttacc
     5221 agcaggtaat aagcacgcat ggatgtgcgc cctgaatgat ttttttaatg accctgggcg
     5281 tttttatcag atggctcgcc aggcaaaaca ggatatagaa gagcggtttg atattaataa
     5341 aactgcgtta aaaatactca cattagcgaa gcacaagtaa caatatgttt tctgtaagta
     5401 acttatcatt tatcggtttc cttaaaagga ttgttttttc ctcagattca ctcccgggga
     5461 agtgggaaca cagaaaattc cggttcatgt acattttgcg atgtgctata aatccggttg
     5521 ccagtattcg atattattac gaactgcgtt ccttgcagtg cattgaggat attctggcaa
     5581 tacaacccac gttgccagca agaattcatc gaccttattt gcataaaggg gggcgagcct
     5641 ggtcccgggg gcaatatatt cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat
     5701 attctgaatt tttgtttcca caaaaatcgg tatcattagt ccaattcatc gggaaagatg
     5761 gagaggattt tgatatccag tgttctccca gtggttttga ccgggaagga gagttgatgt
     5821 tgtcattgtt tttcaataaa atagtgattg cccggttaac attttcagtc attctgactc
     5881 aaaatggaca caccgctttt attggcggat tacagggagc ccccaaaaat accggacctg
     5941 atattattcg gtgcgcaacc cgggcctgtt atgggctttt cccaaaacgg attatttttg
     6001 aagcattttg tgcgttgatg aaagcttgta atgtctctga gtgtctggca gtcagtgagc
     6061 acagtcatgt tttccggcaa ttgagatact ggtatcagaa acgcaaaacc tttgttgctg
     6121 tctacagtga tttctgggag tctgtagcag ggaaaacctg tggtgactgg tacaaattac
     6181 caacacaggt ggttcgaaag ccgctaagca atatagctag taaaaaacgc tctgagtatc
     6241 gaaaacgata cgctttgctg gattatatcc atgaaactgc tatccgctct ttggatgcat
     6301 atcctgtaaa ctcagaacat caggacttaa attaaggttt gatgtattct ctgaataaaa
     6361 tattaatgat gattttggta ggggcattcg cactaaataa tgaaaaaata caaatccgag
     6421 tttattcctg aatttaagaa aaattatctt tcccctgttt actggtttac atggttcgtt
     6481 ttgggaatga ttgcaggtat ttcaatgttt cccccttcat tcagagatcc tgtcttggcc
     6541 aaaatagggc gttgggtggg cagattgagc agaaaagctc gtcgcagggc gacgattaat
     6601 ttatcgcttt gtttcccgga aaagagtgat acagaacggg aaataattgt cgacaatatg
     6661 tttgccacag cattgcaatc catagtgatg atggcagaac tggcaattcg tggtccggaa
     6721 aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc ttgaggaaat tcggcacaat
     6781 aacagaaatg tgatttttct ggttccccat ggctggagcg tggatattcc tgcaatgttg
     6841 ctggcagctc agggggaaaa aatggccgcc atgtttcatc agcaacgaaa tccagtgatt
     6901 gattatgtct ggaattctgt acggcgtaaa ttcggggggc gcttacattc ccgggaggat
     6961 gggataaaac catttattca gtcagtacgc cagggatact gggggtatta ccttccagat
     7021 caggatcatg gtcctgaata cagtgaattt gctgattttt ttgcgaccta taaagcgaca
     7081 ttaccaatta ttggacgtct gatgaacatc agtcaggcta tgattatacc gcttttcccg
     7141 gtttatgatg aaaaaaaaca tttcctgact attgaagttc ggccaccaat ggatgcatgc
     7201 attgccagcg cggacaataa aatgattgcc cgacaaatga acaaaacagt ggaaattttg
     7261 gtggggtcac atccggaaca gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca
     7321 aacgaagcgg acccgtaccc ttgaaattat aaagtacagg tatttccact agttgtttct
     7381 tacaggttac caatcgaaac acatccccct tccgtgtgtt gcccgtttgg cacggaggac
     7441 tgtgtagatg cagttcgtta aatttaattt atgcctaaat agctgcgctt aataccgcta
     7501 cacttttgcc agcccatgtt tgcctccggg gaatgggctg acggtttcca taaaatggcg
     7561 aacttttttc aacagttgcc acattgagcg gcactgatga ttacgcgtta ttgtgtcgtg
     7621 aagtgcctgc catagccgtt caacatgatt cacccatggc gagtaaaccg gctgataaat
     7681 gcccctgaac ttcgggttct ccttcagcca gctctgtgtt tcccggcttt tgtggataat
     7741 gtagttgtcc acgatcagcg tgatggtttt cgcacgacgg tatgtcgctt taagccgctt
     7801 cagcaggctg atgaacagcg ccgaactttt gctgttgccg cccacatagc tgactttacc
     7861 tgtcccgctg tgcagcgctc cggccagata atatttttca ttctgtcccg gcgtgaccac
     7921 ccgtttttgc tgtccgcgca gttgccagtc cgcaccgatt ttgggatgaa gatggatatc
     7981 cacttcatct tcataaaaga ccggatgctc tgcgctgcat tcgtccagtg ctttatggat
     8041 tgctgccatc ttttcatctt tatgcgggtc acggatacgc agagttggcg cagcccttcg
     8101 ccacacaatc cccacagacg gcaaccagcg gcgaacggtt ccggcattta actggca
//
LOCUS       DAJVPT010000146         8152 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVPT010000146 DAJVPT010000000
VERSION     DAJVPT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..892)
                     /locus_tag="OQM47_003546"
     CDS             complement(<1..892)
                     /locus_tag="OQM47_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1481562.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATY"
     gene            complement(993..1253)
                     /gene="iraP"
                     /locus_tag="OQM47_003547"
     CDS             complement(993..1253)
                     /gene="iraP"
                     /locus_tag="OQM47_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1481563.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(1435..1638)
                     /locus_tag="OQM47_003548"
     CDS             complement(1435..1638)
                     /locus_tag="OQM47_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481564.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            1716..2810
                     /gene="ddlA"
                     /locus_tag="OQM47_003549"
     CDS             1716..2810
                     /gene="ddlA"
                     /locus_tag="OQM47_003549"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1481565.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(2834..3046)
                     /locus_tag="OQM47_003550"
     CDS             complement(2834..3046)
                     /locus_tag="OQM47_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1481566.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3306..3614
                     /locus_tag="OQM47_003551"
     CDS             3306..3614
                     /locus_tag="OQM47_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1481567.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(3673..4767)
                     /gene="yaiW"
                     /locus_tag="OQM47_003552"
     CDS             complement(3673..4767)
                     /gene="yaiW"
                     /locus_tag="OQM47_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1481568.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(4780..6000)
                     /gene="sbmA"
                     /locus_tag="OQM47_003553"
     CDS             complement(4780..6000)
                     /gene="sbmA"
                     /locus_tag="OQM47_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1481569.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6352..7509
                     /gene="ampH"
                     /locus_tag="OQM47_003554"
     CDS             6352..7509
                     /gene="ampH"
                     /locus_tag="OQM47_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1481570.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(7510..8013)
                     /locus_tag="OQM47_003555"
     CDS             complement(7510..8013)
                     /locus_tag="OQM47_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1481571.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2052 a         2075 c         1931 g         2094 t
ORIGIN      
        1 ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca aacaggccaa
       61 gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca tcgttcacca
      121 actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat tcaccagcgg
      181 ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca cgagcgttaa
      241 gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc ggacattttt
      301 cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc agcgcagcgg
      361 gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct gcggcttttg
      421 ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc gcgccgttat
      481 aggttttgac atcggttgac caggcggttg ctgatgcagc cgagtcggtg acgtagtccg
      541 gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc ggtaaggcat
      601 cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca gtaatttccg
      661 aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta tcgctaagag
      721 aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg ggtgcagtaa
      781 tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg gcttttgtca
      841 caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc actttatttt
      901 ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc ggccgtgaca
      961 actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt caataacttc
     1021 tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc gggctttacc
     1081 tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg cgccatattg
     1141 cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag ttctttcgac
     1201 tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt catgtctgta
     1261 tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat agtgtctgga
     1321 agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt gattgccagc
     1381 gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta attatcaacc
     1441 tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat taagagaaaa
     1501 aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca tcatttttgt
     1561 gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc acgctttgtc
     1621 agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg
     1681 tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg
     1741 tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg
     1801 atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat
     1861 ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt
     1921 tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat cagcttatcg
     1981 acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta
     2041 cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag
     2101 gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa cgtctgctac
     2161 gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca
     2221 tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa ccggctaatc
     2281 agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca attgccgtcg
     2341 atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa ggtcgtgaga
     2401 tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac
     2461 tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg
     2521 tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt gccgttcagg
     2581 cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga
     2641 acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc
     2701 cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt ttgattgagc
     2761 tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa tattttaacg
     2821 cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaacc
     2881 ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt
     2941 ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg ccaatggcga
     3001 acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca gctccgttaa
     3061 cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac aacattttcg
     3121 taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga agattcacgc
     3181 ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct
     3241 gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg
     3301 tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct
     3361 cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg
     3421 cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc
     3481 aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt ttcgggctga
     3541 tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg cactggcgac
     3601 atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat ctggcctgac
     3661 attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa
     3721 ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc caggcaacat
     3781 ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag
     3841 cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa tttcgctgtc
     3901 gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc cgggttcttt
     3961 actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac
     4021 tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaagcg
     4081 gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac caaaccacaa
     4141 cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc acggataccc
     4201 tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg tgcgcaccgg
     4261 attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat cgtcaaaaat
     4321 cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac ttttgccgtt
     4381 tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca tccgttcagc
     4441 acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg gatctgcctg
     4501 ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca gcgtcggtgc
     4561 cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc ggtctttcac
     4621 gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt cgcccttttt
     4681 caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca acaatgacag
     4741 cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg tatgggttac
     4801 ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt tgtagataga
     4861 catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac cgcgaacctg
     4921 accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga
     4981 cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat
     5041 gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg cgctaaacag
     5101 ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt
     5161 acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt tgatccctac
     5221 cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca ccagaccata
     5281 cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta ccagcaccgg
     5341 caggaaggcg atcaacgtca tgatggcgtt gataaaactg acgcccatat tctccagcgt
     5401 tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga tatgacgcag
     5461 ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga acacgtagtg
     5521 actgacaaag aagttgttca gtacactgat caccaccgcg atcagcgcaa tccccagaaa
     5581 gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac ttagcgcggt
     5641 ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga cttccaccaa
     5701 aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt gccaacggtg
     5761 cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt agtaagcgta
     5821 aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct gcccggaagc
     5881 gccggtgata cgtgccaccc agtcaccccc accggcttgc cagaagataa cggcaatcaa
     5941 tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag acttaaacat
     6001 atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccgc
     6061 tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc
     6121 cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt ttacataagc
     6181 ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt
     6241 acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat
     6301 gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc gttgaaacgt
     6361 agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag
     6421 ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt
     6481 tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt
     6541 cgtagttatg gtgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg
     6601 cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt gctcgaccag
     6661 gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgccg
     6721 acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag cgccctgccc
     6781 cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa
     6841 cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac
     6901 tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat
     6961 acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac cacctacacc
     7021 ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat
     7081 aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc
     7141 tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg
     7201 cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc
     7261 aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgagg
     7321 attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat gatcccgcag
     7381 aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg
     7441 agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc
     7501 gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat
     7561 ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat
     7621 gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt cttcattcca
     7681 gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat atcccgtaca
     7741 atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc
     7801 cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac gcagagagat
     7861 aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa atataacttg
     7921 cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc
     7981 aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt
     8041 caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga gtgaagaata
     8101 ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt ca
//
LOCUS       DAJVPT010000147         8085 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVPT010000147 DAJVPT010000000
VERSION     DAJVPT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(111..968)
                     /gene="murI"
                     /locus_tag="OQM47_003556"
     CDS             complement(111..968)
                     /gene="murI"
                     /locus_tag="OQM47_003556"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1481572.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(913..2757)
                     /gene="btuB"
                     /locus_tag="OQM47_003557"
     CDS             complement(913..2757)
                     /gene="btuB"
                     /locus_tag="OQM47_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1481573.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2822..3012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3126..4226
                     /gene="trmA"
                     /locus_tag="OQM47_003558"
     CDS             3126..4226
                     /gene="trmA"
                     /locus_tag="OQM47_003558"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1481574.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4266..4625)
                     /locus_tag="OQM47_003559"
     CDS             complement(4266..4625)
                     /locus_tag="OQM47_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1481575.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4625..5275)
                     /gene="fabR"
                     /locus_tag="OQM47_003560"
     CDS             complement(4625..5275)
                     /gene="fabR"
                     /locus_tag="OQM47_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1481576.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5596..6996
                     /gene="sthA"
                     /locus_tag="OQM47_003561"
     CDS             5596..6996
                     /gene="sthA"
                     /locus_tag="OQM47_003561"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1481577.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6979..7896)
                     /gene="oxyR"
                     /locus_tag="OQM47_003562"
     CDS             complement(6979..7896)
                     /gene="oxyR"
                     /locus_tag="OQM47_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1481578.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
BASE COUNT         2087 a         2147 c         1975 g         1876 t
ORIGIN      
        1 cggcgtgttt gccgttgttc cgtgtcagtg gtggcgcatt atagggagtt attccggcct
       61 gacaagagga aatttaaaat aattttccga ccgcgcaaca ttcaaccaaa tcagcctaaa
      121 actgccagtt tttcgagcgt ttcgaagccg taacgctgta aaacgggcaa taattgttca
      181 gcttctggcg tcattgccat acaaaaggca atattcgcat cggcagattt tgcatccggg
      241 gcttcatgtt ctaacaacca ggccgttcgg cgagcaatcg ctgcgccaga atccaccagc
      301 cgggttccct ctggcagcac ttgtaacagt tcttcttgta gtagagggaa atgggtgcaa
      361 cccaatacaa cggtatctgg cggctctttc attcttaacc acgggcgtag gatacgtttt
      421 agtgcatcca gagaaacatc ttcgccatgc agcttcgctt cagccaactc aaccatctct
      481 gccgagccca gcatttctat ctggcattca ttagcgaaac gcgcgatcag ctcatgagta
      541 taagaacgtt taactgttcc gcgggttgcc agtaatccga caatgccatt tgccgtcaga
      601 cgtgcagcag gtttaatcgc cggcacgaca ccaacaaccg ggaagtcgaa cttttcgcgt
      661 aatgcaggaa gtgaaacggt actggcggtg ttgcaagcga ccacagccag cgcaagggga
      721 taacgttctt gcaccgcggt gacaattgcc accactcgct caacaataaa cgcttcgctt
      781 ttttcgccat acgggaaagc gacgttatcg aaagcataaa tgtaatggag atccggtaag
      841 agatgccgga tctcgtcata gaccgacaac ccaccgacgc cggagtcaaa caccagcacg
      901 gtgggacgtg gttcagaagg tgtagctgcc agacaaggtg tattcccgtc ctgcagtttg
      961 gtagccatag actgtctcat aatctttgtc gaacaggttg gctattttac cacgaactgt
     1021 caggtgagag gtgaccggat acgcaaccgc aagatcccac aagctcacac cgcccatttt
     1081 aacggtttga taaggataag atgagtaatc cttatcatag cgagtgccta aatactgata
     1141 agtaataccc cagtcgaagt catacaactg ccagtcgagc tggtatttca cctgctgttt
     1201 agcacggcgt aacaacggcg tgtcggtaat cgcattgcgc gcatcgacat aatcataact
     1261 cacagtatgc gtcagtggtc cggtatcaaa attggctgtc gcctcgacac ccttaatccg
     1321 cgctttccct tcgttgtaat atttcagggt gtgatcatca taatcgatca agtcactgac
     1381 atcgttacga tatccggaaa tacgccagtt caccccagcg gttaagcctt caaacgcgcc
     1441 ttcccactgt ttgcttttct ccgggtccag attcggattt ccgtagaagc catacagttg
     1501 ccccagattt agtgccttat aagatgtccc gtaggaagca atgaagcgat aaccttcgat
     1561 gaattcccaa ccggcgctgg tttgccaggt tccatgacga ccaaactgtg agttatcgtc
     1621 gctgcgtgct gcgccttcaa aggtaaaatc gccgacttgt tgcagcccgg tcagatagat
     1681 gccggtatta cgttgatcat atccatcctc aacataacct gtacccggcg tcgtagtctg
     1741 tttctgccag tcgacacccg caccaatact accgtgacca acgatgacat tgtttgccca
     1801 ctggacggtg tattgcttca tctcatcgag cgtcgccgac gaatcataac gaccataatg
     1861 gggatcgtag ttgtaatctt tgctatggct atagctggta atgagttgtg atttaatcag
     1921 ttcgccgtta tagcgcagcc cggcgtccca actttggcta tagagtttac gggtatcgag
     1981 caacggtgaa ccgggagaat aatacgcgtc ataattggta cggttatcat agccatagcc
     2041 gcgaacaaag ccgctccagg catcagtaaa gttatgctcc agcgccccat aaagcgtttt
     2101 acttaaaaaa ccatcgttat ctgtctgcgc ttgcgttccg gtattaccat aggcaacaac
     2161 atcataacca tgagtatggg cataatcgcc caacagcgtt acccgtgttt tatcccccag
     2221 ttgttgctgc gtagagacat catagttctg ataactattg cttccccacc ctgctgaaat
     2281 ttccgttccg ggttcatcgc gcgtcgtgat gatattcacc accccgccta ttgcatcgga
     2341 accataaaca gcggagcgcg gtccacggat atattcaata cgctggacaa gcgcaatagg
     2401 gaactggcta aggtcggcag aaccactcac ccccgccaga ttcaggcgga cgccatcaat
     2461 taacaccagc acatgactgg catttgtacc gcgaataaaa atagatgaga gctgacctga
     2521 accgccgttt tgggtgatat cgacgcccgg aagacggcgc agcacatcat tgaccgaggt
     2581 cgactgccag cggtcgatat cctgacgggt cacaacggtg gttggtgcaa gcacagtgct
     2641 gcgcggctgt tcaaaacgga tagcagtaac gacgagagta tccgggctgg tatcctgtgc
     2701 ccaagcggaa aatgccgtga cggaacacgc cgtcagcagc gaagcttttt taatcattgt
     2761 aaagcatcca caatagaaga aggatgccgc aggtttcatc aatattacgc gatgatgaga
     2821 accagatgcg acgttggccg gcaggtcttc gggcttggag gggtatctaa gatactaaga
     2881 gatgatgact tcccaccgga tggcagtgtc cgcataacgc aatcatcgca cctttcctta
     2941 ccgctgcgcg tcagctctag attcgcactg gattccctat taactcacag gaccggcaag
     3001 tggatgctac aggttgtaac aagttactgt ccagacgtag ctcacaaata ggaattcatc
     3061 aagatctgga catctgatga gcaatcccta caatcgccgc gtactttaat ttttcaggat
     3121 acatcatgac ccccgaacac cttccaacag aacagtatga agcgcagtta gccgaaaaag
     3181 tggtacgttt gcaaagtatg atggcaccgt tttctgacct ggttccggaa gtgtttcgct
     3241 cgccggtcag tcattaccgg atgcgcgcgg agttccgcat ctggcacgat ggcgatgacc
     3301 tgtatcacat cattttcgat caacaaacca aaagccgcat ccgcgtggat agcttccccg
     3361 ccgccagtga actcatcaac cagttgatga cggcgatgat tgcgggtgtg cgtaataatc
     3421 ccgttctgcg ccacaagttg ttccagattg attacctcac cacgctgagt aatcaggcgg
     3481 tggtttccct gctgtaccat aaaaagctgg atgatgagtg gcgtcaggaa gcggaagcac
     3541 tgcgcgatgc actgcgcgcg cagaatctga atgtgcatct gattggtcgg gcaacgaaaa
     3601 ccaaaatcgc gctggatcaa gattacatcg acgagcgtct gccggtcgca gggaaagaga
     3661 tgatctatcg tcaggtagaa aacagcttta cccagccgaa cgcggcgatg aatattcaga
     3721 tgctggaatg ggcgctggac gtaaccaaag gctcaaaagg cgatttactg gagctgtact
     3781 gcggcaacgg taacttttca ttagcgctgg cgcgcaattt tgatcgggta ttagccaccg
     3841 aaatcgccaa accgtcagtc gctgccgcgc aatacaacat tgccgctaac catattgata
     3901 acgtacaaat tattcgtatg gcggcagaag aatttactca ggcgatgaat ggtgtgcgcg
     3961 agtttaaccg cctgcaaggg atcgacttaa agagctatca gttcgaaacc atttttgtcg
     4021 acccaccgcg cagcggtctg gacagtgaaa ccgagaaaat ggtgcaggcg tatccacgta
     4081 ttctgtatat ctcctgcaat ccggaaacgt tatgcaaaaa tctggaaaca ttaagccaga
     4141 cgcacaaggt cgaacgtctg gctctgtttg atcagttccc ctacacgcac catatggagt
     4201 gcggcgtatt actgaccgcg aagtaaaacc catgccggat gcgccagcat ccggcatgat
     4261 accgattact cagcaacttc ctgcttacgg ttacgcatct tcgcgccaat ccagaacacc
     4321 ataatgacgg agagtactgc cgggaagaag ttagagccga tatccggata ttccgcacga
     4381 actaccgtgc tgtacagcag tacgccgaga ataaaacagg cagctgccag acccggcaag
     4441 cctaccgaca tggtgcgatt aagataacgt tgatgcaggc agtaaaccgt cagcaccagg
     4501 gaaataatcg ggaatacaga aaatggcaca atggagctaa acagcgctgc gaaagtacca
     4561 ttaatcgata aaccagcaac caacgccagc agcagcgtac ctctatcttg atttgcttgt
     4621 ttcattactc gtccttcaca tttcccggaa taattgcggt tttctcttgt tcacggcgat
     4681 accagtaata agcccctttc gaaatcattc gcagttgcag taccagtcgc tcttctaatt
     4741 gccgacgttg ttcgacgccg acgtccaacg cctcggcacc cgcactgaag acaattgtca
     4801 ccattgcttc ggcttgcgct tcagtaaacg cacgcggcat atggttttcg agttccagat
     4861 agtccgcaag ttccgcaatg aagtgctgaa tttcacgcgc aacggcggca cgaaacgcag
     4921 cggaggtgcc ggagcgttcc cgcaataata accggaaggc gttaggatta ttaccgatga
     4981 actccataaa tgtggagacc gaggtacgga tcacactccc gcctttggcg atacgctgac
     5041 gcgcctggcg catgagttgg cgtagcatta aaccgctctc atcaaccatg gtcagaccca
     5101 gttcgtctac gtcgcggaaa tgccgataaa aagaggtggg agcaatgccc gcttcacgcg
     5161 ccacttcacg caaactcagg ctggcgaagc tgcgttcagc acttaattgg ctaaatgcgg
     5221 cttccaccag cgaacggcgg gttttttctt tttgttgcgc tcttacgccc atcacgatgt
     5281 ctgaatcctt gccaaaagta ctgctggcac tataccagag aatgaacata ctggattgct
     5341 ccagtattgt gaatgaacgg taacgcaata aaacatattt actggccggt tcttttacgt
     5401 acagcggaaa cctgccgctt aaacggagag tatcgtcgat aaaaatccaa taaaacgtca
     5461 gggcaaaagt aagaaacaga caaagcaaag gccgctcagg atatagccag ataaatgacg
     5521 gggatcaatt ggcttacccg cgatagaatg ttaccattct gttgctttta tgtataagaa
     5581 caggtaagcc ctaccatgcc acattcctac gattacgatg ccatagtaat aggttccggc
     5641 cccggcggcg aaggcgctgc aatgggcctg gttaagcaag gtgcgcgcgt cgcagttatc
     5701 gagcgttatc aaaatgttgg cggcggttgc acccactggg gcaccatccc gtcgaaagct
     5761 ctccgtcacg ccgtcagccg cattatagaa ttcaatcaaa acccactata cagcgaccat
     5821 tcccgactgc tccgctcttc ttttgccgat atccttaacc atgccgataa cgtgattaat
     5881 caacaaacgc gcatgcgtca gggattttac gaacgtaatc actgtgaaat attgcagggg
     5941 aacgctcgct ttgttgacga gcatacgttg gcgctggatt gcccggacgg cagcgttgaa
     6001 acactaaccg ctgaaaaatt tgttattgcc tgcggctctc gtccatatca tccaacagat
     6061 gttgatttca cccatccacg catttacgac agcgactcaa ttcttagcat gcaccacgaa
     6121 ccgcgccatg tacttatcta tggtgctgga gtgatcggct gtgaatatgc gtcgatcttc
     6181 cgcggtatgg atgtaaaagt ggatctgatc aacacccgcg atcgcctgct ggcatttctc
     6241 gatcaagaga tgtcagattc tctctcctac cacttctgga acagtggcgt agtgattcgt
     6301 cacaacgaag agtacgagaa gatcgaaagc tgtgacgacg gtgtgatcat gcatctgaag
     6361 tcgggtaaaa aactgaaagc tgactgcctg ctctatgcca acggtcgtac cggtaatacc
     6421 gattcgctgg cgttacagaa cattgggctg gaaactgaca gtcgcggaca gctgaaggtc
     6481 aacagcatgt atcagaccgc acagccgcac gtttacgcgg tgggcgacgt gattggttat
     6541 ccgagcctgg catcagcggc ctatgaccag gggcgcattg ccgcgcaggc gctggtaaaa
     6601 ggcgaagcca ccgcacatct gattgaagat atccctaccg gcatttacac catcccggaa
     6661 atcagctctg tgggcaaaac cgaacagcag ctgaccgcga tgaaagtgcc gtatgaagtg
     6721 ggccgcgccc agttcaaaca tctggcacgt gcacaaatcg tcggcatgaa cgtgggcacg
     6781 ctgaaaattt tgttccatcg ggaaacaaaa gagattctgg gtattcactg ctttggcgag
     6841 cgcgctgccg aaattattca tatcggtcag gcgattatgg aacagaaagg tggcggcaac
     6901 actattgagt acttcgtcaa caccaccttt aactacccga cgatggcgga agcctatcgg
     6961 gtagctgcgt taaacggttt aaaccgcctg ttttaacact ttatcgaaat ggccatccat
     7021 tcttgcgcgg atggcctctg ccagctgctc atagcggctg cgcagcggtg agccaggacg
     7081 ataaaccagg ccaatagtgc ggcatggttc cggcttaatg catggcagat aaacaacccc
     7141 atcgcgtttg cgctccggcg gcacagccag cgctggcaat aaagtgatcc cgctacctgc
     7201 cgccaccatg ttgcgcagag tttccaggct ggtcgcgcgg aagtgtgtat cttcatccgc
     7261 cccggcttca aagcagaaac ccattgcctg atcgcgcaaa cagtgaccat cttccagcat
     7321 cagcagtttt tcccctgcca gatcggccat cagtacgcat tcgcggttcg cccacgggtg
     7381 atcttcatag atagccagca acattggctc atcaaacaac ggcacttcaa tgaatgcttc
     7441 gctctctttc accagcgcga ggatcacgca atcgagtttg ccgctgtcca gttgcgccag
     7501 taactggtgg gtctgtgctt catgcagata catttccagc tttggaaatg tctggtgcag
     7561 catcgggata atatgcggta gcaggtatgg tccaaccgta ggaatcaaac caatatgcag
     7621 cggtccggac atcgtctcac cctgctggct tgccatctct ttaaggactt tcacctcacg
     7681 cagcacggta cgcgcctgat ccaccagcag cattcccgcc tgggtgaaca acactttacg
     7741 gctggtccgc tccagcaaca tcacgcccag ctcatcttcc agcttacgaa tttggccgct
     7801 aagcgtcggc tggctaacgt ggcaggaatc tgccgcacgc cgaaaatggc ggtgttcagc
     7861 caatgccacc aggtactcaa gatcacgaat attcattatc catcctccat cgccacgata
     7921 gttcatggcg ataggtagaa tagcaatgaa cgattatccc tatcaagcat tctgactgat
     7981 aattgctcac agaaacggag cggcacctct tttaaccctt gaagtcactg cccgtttcga
     8041 gagtttctca actcgaataa ctaaagccaa cgtgaacttt tgcgg
//
LOCUS       DAJVPT010000148         8003 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVPT010000148 DAJVPT010000000
VERSION     DAJVPT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..1322
                     /gene="yjeM"
                     /locus_tag="OQM47_003563"
                     /pseudo
     CDS             1..1322
                     /gene="yjeM"
                     /locus_tag="OQM47_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OQM47_003564"
     CDS             complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OQM47_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1481579.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OQM47_003565"
     CDS             complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OQM47_003565"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1481580.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OQM47_003566"
     CDS             complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OQM47_003566"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1481581.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7018..7563
                     /gene="orn"
                     /locus_tag="OQM47_003567"
     CDS             7018..7563
                     /gene="orn"
                     /locus_tag="OQM47_003567"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1481582.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7774..7849
                     /locus_tag="OQM47_003568"
     tRNA            7774..7849
                     /locus_tag="OQM47_003568"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7807..7809,aa:Gly,seq:gcc)
BASE COUNT         1827 a         2069 c         2168 g         1939 t
ORIGIN      
        1 gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg gatgaataat
       61 agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc ttatatcatc
      121 tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct ctatggtagt
      181 gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt tggtctgctg
      241 gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa taaaattgcc
      301 cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt gctgttagta
      361 agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa tttccttgca
      421 tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt atttgctatt
      481 tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga aaatccagaa
      541 aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg ttattcgctg
      601 gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa tggttccgtt
      661 aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct gggtaacgca
      721 ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga
      781 ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa gccatcatcc
      841 aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc
      901 cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg ctggtttcgt
      961 ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg aacgtgtcta
     1021 tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca cgtcaggatc
     1081 tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg
     1141 tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg gttgaagccg
     1201 gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga
     1261 tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact
     1321 gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc
     1381 gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga
     1441 gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg
     1501 cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg
     1561 aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat cagctggtgg
     1621 atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa
     1681 atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc
     1741 agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg
     1801 gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga ccagttgata
     1861 aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc ccagtcgctg
     1921 atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg gatcgttacc
     1981 gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg
     2041 aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat
     2101 ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag catcagcaga
     2161 tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa
     2221 atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat gataaatacc
     2281 agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc
     2341 gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct
     2401 gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc
     2461 aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc aattgctcct
     2521 tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc taacatctct
     2581 gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag catccagcgg
     2641 cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc
     2701 gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt
     2761 ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc gctgccttct
     2821 ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac cgccagcgca
     2881 ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgagg
     2941 ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta gcgcatccca
     3001 cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca
     3061 aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac accatcgcca
     3121 atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac
     3181 agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag
     3241 aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa
     3301 tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt
     3361 gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc
     3421 gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc
     3481 acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc
     3541 tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc
     3601 tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt
     3661 tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta
     3721 ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc
     3781 tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc ttcgccgagc
     3841 aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc
     3901 tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat ccgctgcgcc
     3961 tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc
     4021 ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg ctccgcctca
     4081 agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg
     4141 ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac
     4201 agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac
     4261 gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg
     4321 atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg
     4381 gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg
     4441 ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg
     4501 cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa
     4561 ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc
     4621 gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt
     4681 gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc
     4741 caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg
     4801 atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg
     4861 atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt ttcggtagat
     4921 actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc
     4981 actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc
     5041 tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg
     5101 atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca
     5161 atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc
     5221 acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg gttaacggaa
     5281 aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg
     5341 cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc
     5401 gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc
     5461 aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc
     5521 agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat
     5581 tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg
     5641 tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg
     5701 ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta
     5761 aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggccaacat
     5821 gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc
     5881 gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag
     5941 ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc
     6001 ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg
     6061 acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca
     6121 ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg
     6181 tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc
     6241 agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa aatgctgatg
     6301 cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct ggaaaccatc
     6361 aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa cgccatgcct
     6421 tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg caaggtttcg
     6481 caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca
     6541 atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa atccggacgc
     6601 gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc
     6661 gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta
     6721 cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca
     6781 aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc
     6841 ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct
     6901 ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat gggcaaaaca
     6961 tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg
     7021 agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag
     7081 cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa tattctggca
     7141 gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat ggatgactgg
     7201 aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc
     7261 gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc tgcgggaaaa
     7321 tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa atacatgccg
     7381 gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg
     7441 cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg
     7501 gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg
     7561 taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc
     7621 taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt
     7681 ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac gacgcagaaa
     7741 tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt ggtagagcac
     7801 gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa aatttgaaaa
     7861 gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag gcggcaactg
     7921 aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc agccaaggca
     7981 gtagcggttc gaaagacgaa gat
//
LOCUS       DAJVPT010000149         7727 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVPT010000149 DAJVPT010000000
VERSION     DAJVPT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..319
                     /locus_tag="OQM47_003569"
     CDS             <1..319
                     /locus_tag="OQM47_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481583.1"
                     /translation="NQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYG
                     MRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIA
                     MGY"
     gene            291..725
                     /locus_tag="OQM47_003570"
     CDS             291..725
                     /locus_tag="OQM47_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1481584.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1498..2547)
                     /locus_tag="OQM47_003571"
     CDS             complement(1498..2547)
                     /locus_tag="OQM47_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1481585.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            2998..4188
                     /locus_tag="OQM47_003572"
     CDS             2998..4188
                     /locus_tag="OQM47_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCS1481586.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4578..6038)
                     /gene="gtrII"
                     /locus_tag="OQM47_003573"
     CDS             complement(4578..6038)
                     /gene="gtrII"
                     /locus_tag="OQM47_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCS1481587.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6035..6964)
                     /locus_tag="OQM47_003574"
     CDS             complement(6035..6964)
                     /locus_tag="OQM47_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1481588.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(6961..7323)
                     /locus_tag="OQM47_003575"
     CDS             complement(6961..7323)
                     /locus_tag="OQM47_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1481589.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2406 a         1454 c         1529 g         2338 t
ORIGIN      
        1 gaatcagata cctgatatga gcctgttcgc gtcaattaat accgtaacgg ctgctgcgca
       61 aaaatttccg tctggattaa ttttacagtg cggtcagttg aatggtgccc cgaatgtatc
      121 ttcaacatac gggatgaggt tcccgatgac gttctcaaga gtaattgctg tcgtagttac
      181 attgaacgtt actggcgcgg ctgggcagcc gactgtatcg gcgacaagtg tccagaacac
      241 tggatttaat attactgtgt cgcccggttc aggatacggt tcatctgctg atgcgtatta
      301 cattgcaatg ggatattaac aaaatgtcat atttttattc tgcatcgaca aacggatttt
      361 attcgactga atttcacggc accaatattc ctgatgatgc agtggaaatc tcggaatcag
      421 agtggaaaac actgattaat gcacagagtg taacaaaaat gattacctgt ggtgagaacg
      481 gtcatcctgt cattgttgac cgtccttctc caacaccaga acaattagcc ttaataaatg
      541 atgaaaagaa atctgcactg atagcagagg caacgaatgt aatagctccg cttcaggatg
      601 cggttgattt aggtatggca acagatgatg aaacgaaact gttactggca tggaaaaaat
      661 atcgggtgct gcttatgcgt gttaatgtag taaaacccga gtggcctatg catccaaata
      721 aatagacttg ttaatctagg ataattgttg gaataatact cgtttttcag taggttcagg
      781 tagcctgggt ataatgctca cttctacaat gatttcaact tattttattt aattgatatt
      841 acgaaactta actcatacat aaacaatatg actcaatact gtcctaaata gctgcgcaga
      901 aaagtgctcc actttcgctc agcccttgct gggcgaagca tcaggaaggc gttctcagct
      961 aggttgtgtt atatctggct tgagttttgt tgcccatacg caacacatta cccatctgta
     1021 aacgacaagt gtggcagaat taggtgcagc tatttagact atatcgacgc agtaacgata
     1081 atagatatcg aaactgtact agatattagc tacttatggt taccgggggg agtcttttga
     1141 gcactggtgt tatcaattgg cttcaaataa ttcgccaata aattatattg cgatagctca
     1201 tcaccttcca ggttgtttat agccagttta tcagcgtcac cacctactgt ttatgctttt
     1261 atttctttga ttaataagta tatgatattt taattcatct ggtgtatttc tttctcatgt
     1321 gttggtcgac tgaatatctg ttcagtgaag gggtaatttt atgtttgcac ttcgaacatt
     1381 cccttcagtt gctaggaaag aaaaccgtaa catttgcgca ttaattttaa cagtataatc
     1441 tgagcataaa tatgcgctat atatctataa aaaggcgcaa ttaattgcgc ctttttatca
     1501 ttttatcaaa aatggcccac cgactgtcct cgatagccaa tggccaattt tatctgatac
     1561 atatacacaa atacaggtat atatgattgc gcagatagga tttggaatag tcacgctgaa
     1621 gcctataatt gctaaagcca tagggataaa taatttggtg atatactcca ttccacatat
     1681 gttaagagta tttcttccag ctcttacaat aaagtcattt ccacaaatta tttttgctat
     1741 gaaaatatta aagattatca agcctaccgt ggtgataata gtattaatag cttgaatata
     1801 actacttgat attatgtctg cattgaatat tgagataatc ttaaaaagta aaggtttctg
     1861 gttaagtagt tgatacgcac tcattatagc ggtaattgag aagacaatga aaccaatttt
     1921 agattttcca aaaaacctgt cacgtgttaa ttctaaaaac atacatctac cgagaggcaa
     1981 taaccaccag tatgccatag cggagtctat gttccaaaac cattgaggat ctagtaatgg
     2041 gttatgattg agcactgttt gtgacaacat gaacgataac agtgatatga ttaaaatcac
     2101 tatattgttc ttgactattt ctctaaagat tgcatcaatt gctattataa caaaaaggca
     2161 gtttataaac catattgttc ctacgaattg gttatttcta acaccatata ttatatcata
     2221 aatgtggttg tatatataat caatcgtctc acctgagtta attgcgttta ttattaatat
     2281 agaaaatgca aaagtgaaat atggaatcat taatctgtaa aactttgact tgatataatc
     2341 gaaaacagat aagtcatttt ttttgatagt aaaaaaacca gcagcgaaaa agaatagtgg
     2401 gacgtgataa ctaaaaacaa aaggatacaa tttgccagcc cccaacccaa ggtgacctaa
     2461 atatatagca aaaataccga gaaattttaa agcatcaacc caatcatgtc ttatttttct
     2521 gttgctgtcg attttgttat tatacattta aagtaggctc tccagttttg tgtgattttt
     2581 aaacactcat tgcaaatatg aatgcctgaa agtttatgat tattaatcaa cgagttcaat
     2641 attaatttat tatttttcaa agtcgcacta tataggcatt tacttccgca gtaccaccga
     2701 tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc tgccagcggc
     2761 tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag tttcaggcaa
     2821 tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct ttacgatctg
     2881 catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag aaaagaagac
     2941 aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat cacctctatg
     3001 cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac gcatcagtat
     3061 cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc tctttctctt
     3121 accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat aaaacgcgtt
     3181 gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt caaaaatatt
     3241 acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg gagcggttac
     3301 ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg ctctatcccc
     3361 ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat cgcttttctg
     3421 gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac tgatgctggt
     3481 tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat tggtcgtatc
     3541 aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga cataaaaatg
     3601 tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg gaaaaaacgc
     3661 tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag aaaaagcagg
     3721 cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc cgcaggcaga
     3781 gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat catgaaactg
     3841 tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga acgctttggt
     3901 tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt aagtctactg
     3961 gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa taaaggattt
     4021 catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt tctgacgctg
     4081 gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc tgaaaagata
     4141 ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttactgatg aaaattttct
     4201 ggggatccct cagcctttcg gggaggaatg agggctctga tattcttttg ccgtagttca
     4261 tcgtgaaacc gtcggatgtc atttggcctg agtgacccat gcctcccaaa agcagagtcg
     4321 ctagtgttat gtaattaggc ggttaaactt caattctcag tgttaagttt gggtaaacgc
     4381 ttgtaaatgg tattgctaat tttttaattg actaatatta gaaacggtta gtgtgattgt
     4441 atatagatat ttgagtgtaa ggtatttaaa ggggcattca gcccctccgt ctatttgtga
     4501 ttgcatccat tgatgtgtgt attattttga gcttgaaaaa gggaggcgct ctttaataat
     4561 aaataaatct atcaaagcta aatattaaat ggaagccacc cttttttctc tgatagtttt
     4621 attatcacca aaccatttat ttcagctatc gaatctggat tcggccagga aggtaaactc
     4681 ttcacaattg gataaatctt ctctatttgt tctttatttg ctggtttgca attacagatt
     4741 ccatagtaat tcataaatgc aaccatccta aaatagttgc caccatccca ccaaaaaaaa
     4801 gattttccaa aagtgtctgc gctcatggag agcatatttc ctgagtcgtt tgaaccatat
     4861 atatatatat attttccgtt aagggaaatg ccttttgttt gagatgtgta tgatatttct
     4921 ttagctaaag agatatcttt ctgtcttgcg agagtatcat tcaaaaatag atttttggat
     4981 gttgaaacgc cattaaatat aataaataaa aaaaacatgc agtttaggta ttttatagag
     5041 cgaaaattgc ttaagcaaga aaacaaaatt actgctacta taggcattaa aacaaacagg
     5101 cgaggtggcg cccctgagcc aacaacaacg ataaatataa ccggaagtat ataaatgaaa
     5161 atgattgatg aaatcaaata aattgatctt gtttttaatt tatatgttag ggtaaacatc
     5221 agaattaata aaggaatcag taaccacttg tataggttta aaccagtggg aggattgtta
     5281 tagaagttat atatattatc tatggctgat ttaacaagcc atttaatatc gcttgcattt
     5341 tgtatataat ttgagatgta ctcgttcgat tcaagcgaaa aataatgctt gatagctttg
     5401 gttaatagca ggtaaattaa ggtagataaa gctatgaggg atagagataa acatgaacta
     5461 taaaaagatt cacgaatatt tttctcattt cgtattatcg aatttatctg caacccaatg
     5521 acgaatgcaa taatatatgt tacgaatgtt tggtagattg ccattgaaag aatggacagt
     5581 actataccag aaaatatcac aatcctattt ttttgcgagt gaaaaataat tgctgatatc
     5641 gctgccagta gaaaagcaat tcccacagta tcagcttggt taagaaactc taattgatag
     5701 gagatctgag ggaaggtaat aaatactaac atacctatca ataattcata agattctagc
     5761 tttagcgatc tgcagattat aaatgctgaa atgatgataa aagataaggc tattaatggt
     5821 gttatataaa gtgaaaaagg ctcagggaga aagtaatgtc gcaaaaaagt gtgaaaccac
     5881 cgcccaagcg tgattgtttg ataaaaatta tttgtaaact cgccatcaat attaagtgtt
     5941 ccatcgtaca accaccccca ataacaataa attgatattg caaaacatga tatgaaggca
     6001 aggagatttg catttttaaa taaattaatt ttaatcatgg gttacctcga tgagattttt
     6061 ttatgatgta tttgggtcta ttttttactt caatatagat tctacctata tattctccga
     6121 gaacaccaat cccgatcagt tgcactccac ccaagaaaag tattgataca agcagggagg
     6181 gatacccgcg tactgggtta ccaaagacaa gggtgtctat aatcatccat gcaccatata
     6241 aaaatgaaat gcttgcaaca aacaagccta tataagtcca tacgcgaaga ggaaaggttg
     6301 aaaaacttgt gataccttcc agtgccagat tccataattt ccagccatta aattttgaga
     6361 tgccagcaac acgctctgcg cgtacatatt caacgacatc cgtcggacca cccacccagc
     6421 tcagtatgcc tttcatgaaa agattgcgct caggcaacag tttaatgttc tccacaacct
     6481 cacgagacat gagtcgaaaa tctccgacat tttcctcgat ctttggggtg cttattttgt
     6541 tgtgtaattt atagaaccac tcagctgtct tacgtttcag tcgtccatca gttgagcggt
     6601 ctgaacgttt agcaagcacc atgtcagcac ctgcctgcca cttttcaata agatgaggga
     6661 taacctcaat tgggtcttgc aggtcgacat caataggaat tacagcatcg ccgcttgcat
     6721 ggtctaaccc tgcaaataag gctggttctt taccaaagtt gcgtgtaaat gacagcggaa
     6781 caactagcgg gtctgaaaca gccagcgcgt taataattga ctctgtggca tctttacttc
     6841 cgtcatttat gaatacaatt tctacttcat atggcttcaa ctcttggaat tcacgtaccg
     6901 ttttatagaa aacaggtatc gcttcttctt cattgaagac aggaacgaca agagatattt
     6961 tcatttcgca tccctaaaga caatgaactt tgaatagacg aaaccgcaca ccagactgat
     7021 ggcggagaag gtgataagag tgactattgg aggaagtgag catttatcag ctgcccaacc
     7081 aacaatcaca ctcaatattc ccataaatcc cacgtataac atgtagcgca tcgctgtagt
     7141 caatgctttg aatgtgaatc ttgcattcgc gaagaagcta aagctcacag ccactacgaa
     7201 acctgtgaag tttgccagag cctgactggt atgtgcggca tagatacata caccaaaaac
     7261 cacccagtgt ataagtgtgt tcagcacacc tatagatgtg tactttacaa ataactttaa
     7321 catttattta atcaatgagc tctgaaaggc atgaagtcta tcatccaagt ctcaattgat
     7381 cgatacttgc tatgtctgat gagacaaaac tgagacacat aaggcctcac aatggcttgc
     7441 aaggctttac atgttttgat gtggtgggac gtgtgagcgc agtgttgatg ggataatcct
     7501 ttgaattaca agcggattct tataattcgt aatgcgaagg tcgtaggttc gactcctatt
     7561 atcggcacca tttaaatcaa taagttacct cgcatttaag taaactacgt tctcctcttg
     7621 tgccgtattt gtgccattgc gacttataat cgcatcgatt ttgctcgcgt gctcggtaag
     7681 atgcccggct gaaaggtggg cgtatctttg aaccatttcg agagttt
//
LOCUS       DAJVPT010000150         7716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVPT010000150 DAJVPT010000000
VERSION     DAJVPT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(864..2060)
                     /gene="hemY"
                     /locus_tag="OQM47_003576"
     CDS             complement(864..2060)
                     /gene="hemY"
                     /locus_tag="OQM47_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1481590.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2063..3274)
                     /gene="hemX"
                     /locus_tag="OQM47_003577"
     CDS             complement(2063..3274)
                     /gene="hemX"
                     /locus_tag="OQM47_003577"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1481591.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3296..4036)
                     /gene="hemD"
                     /locus_tag="OQM47_003578"
     CDS             complement(3296..4036)
                     /gene="hemD"
                     /locus_tag="OQM47_003578"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1481592.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWYFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4033..4974)
                     /gene="hemC"
                     /locus_tag="OQM47_003579"
     CDS             complement(4033..4974)
                     /gene="hemC"
                     /locus_tag="OQM47_003579"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1481593.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5361..>7716
                     /gene="cyaA"
                     /locus_tag="OQM47_003580"
     CDS             5361..>7716
                     /gene="cyaA"
                     /locus_tag="OQM47_003580"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1481594.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSR"
BASE COUNT         1769 a         1990 c         1997 g         1960 t
ORIGIN      
        1 atctgttctc caatgacaag tctaaaaact agtattaaga ctatcactta tttaagtgat
       61 actggttgtc tggagattca gggggccagt ctagcagttg acggggtatc tgctgcgctg
      121 gccatgagag gaagagcggc ggcgacagca acaacaagac gtttgggtga aaacgaaaat
      181 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
      241 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
      301 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
      361 agttgatatg taaggcaggt ttattatatc gcgttgatta ttgatgctgt ttttagtttt
      421 aacggcaatt aatatatgtg ttattaattg aatgaatttt atcattcata ataagtatgt
      481 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
      541 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
      601 aataaacgag tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
      661 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
      721 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
      781 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
      841 gctttcctcc gggtgagaag gaactactgt ggcgggttat tctgcaacgt taacatcaaa
      901 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
      961 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ccgcgcggaa ggcgagcgat
     1021 gcttcctgcc attcaccgtg cttcatcagt gactgaccca atgtgctcca caacagcggg
     1081 cgatcaccga cgtttttgat ttgctggcgt agcacttttt ccagctgttc cgggttattg
     1141 gttttcagac gcgggatcgg cagcagtagg cgatcgtcat actggcgttt caggccgtcg
     1201 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
     1261 acctgcaacg ctacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
     1321 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
     1381 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
     1441 agcagagaac tccatgcccc tgtgcggata tacgcctgtt ccgccagacg taatacttca
     1501 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     1561 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     1621 agctccgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg
     1681 gcttcggcag ccagtagata gttcaccacc ggttgttcag cgtgatcggc atttttcgcc
     1741 atcagctttt caacttgctg ataatcacct tccgccagtt tcagcagcgc ctgttcggtc
     1801 tgcttacgcg cacggcgacg cttacgtccg acaaaccatc cacgggtgtg cgcgccagtg
     1861 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaat
     1921 atgatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcggtctg gatcagcaca
     1981 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     2041 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     2101 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tgcaggttta gcttccgttg
     2161 tccccgctgc cggttgtgcc agcaggttac gcacgcgagt ctgcatcagt ttttccagca
     2221 tcgcctggct ttgcagggtt tccggaagat ccatcgagat attttgctgg cttaactggt
     2281 ccacctcgtc gaggaacgct ttggtggtgg catcatcagt atcgtagtaa gcacgtaccc
     2341 aggtggagac gttctccagc gcctggcgat aagtctcttc ctgatgacgc ggtacagctt
     2401 gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag atagatatcc tgatttggcg
     2461 ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt aatgaagttg tccataaagt
     2521 tctgccagct tttttgcaga ttgatacgcc attcgctgat ggaactggaa agctcttcac
     2581 cgtctgaatc catcggcgaa ccatcgctgt cattatcggc cagacgcagg ttatctacct
     2641 gatttgaaag ctgattaagc ttaaggatga tgccgtcata atccacctgc gatactgcag
     2701 aaaggctggc gatatcatcg gtaattgccc gacgaacggt aatcagactc gggtcattca
     2761 tatccgccag gctggcgtct gcacttttca gcaacgctgc agaggtcgtg acgtcctgat
     2821 cgctccacag cttccgtccg gcgagtttca ccagaaaatc ggcctgagcc agcagccagg
     2881 ttttagcatc gctgccggaa atggtggcga tcttttgttg gacttcatcc aactgttttg
     2941 ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc agcttgttgc ttaataatgc
     3001 cttccagctc ggctttttgg ctctcctggg ctttttgcaa tgccgtcagt tggttagcca
     3061 gggcatcgct ggtggcggtc tgattgacgg cctgttgttt accccagcca tacaaaccga
     3121 cacccgccgc cagagcaata gcgatagcca ccgcgctgag aatcaatgca gtattgttct
     3181 tactcttttt ttctgttgcg acaggttgtg acgtggtgtc cacggcctcc ctggtctctt
     3241 caaccacggc ggaggttttt tcttgttccg tcattatggc ttcctgttat gagagttatt
     3301 gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc gaccttaatg tcttgccagc
     3361 ccagttcccg ggcgagtttc gccaaacgct cactgacgac caatagtcga cagtgtagta
     3421 accagtgctc acgataccat tgtgggatca gcgaccagag ttgctgcaac atttcaccgc
     3481 tggtaacaac gaccatcgtc acctcgcggg attgccagcg catcgcttct tctgcaccat
     3541 cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt gacctcagca ccgcgcaccg
     3601 tcagggtatc cccaattagc tcacgaccgc cattgccacg taatatcagc gcacgtttgc
     3661 ccgcaatatt ttgtaattca ggtaattgta gcaagacttc gctgatttcc cgatcctgtg
     3721 ggtagagaat cttctgtccg cttacggtat gtagcgccag tgcggtggtg cgtccaatgg
     3781 cgaaataagt aggtagttgg ggccatttac gatcttgctg atgcagctgt gattgggcaa
     3841 aagcaaccgc gtgttgcgag agggcaaaca acagatcgct ctcccccagc gctgccagtt
     3901 gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc aatcagcgga aaataccagg
     3961 ccacctgccc cagtgtgcgc agacggctca ctaactcttc tccagcggga gacgggcggg
     4021 tgaccaggat actcatgccg gggcctctcc gttatagact tcagcgagga tctcgcgcgc
     4081 gccgttattc agtagctctt ctgccagcga aatccccatt tgttcggcat cttgcggcgc
     4141 accgcggcgt tcaccgcgaa taatctgcga accgtccggc gcgccgacca gcgcacatag
     4201 ccagatttcg ccatcaataa gctcggcgta gctaccaatt ggcacctgac atccgccttc
     4261 gagacgggta ttcatggcgc gttctgcggt aacgcgcagt gcagtttcgt ggtgattcag
     4321 cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg cattcaatac cgactgcacc
     4381 ttgtcctacc gccggaagag aaatctcggg tggtaacgcg gcgcgaatgc gggactccag
     4441 acctaaacgt tttagtccgg ctacggcaag aatgatggca tcgtattcgc cgttatctag
     4501 tttgctcagg cgagtgccga cgttgccgcg cagggagcgg ataatcagat ccgggcggcg
     4561 ttcagccagt tggcactggc gacgtaaact ggacgtcccg acgatactgc ctgctggtaa
     4621 cgcatccaga ctgtcatagt tattggacac aaaggcatcg cgaggatctt cacgctcgca
     4681 aatagtgacc agccccagac cttgcgggaa ttcaaccggc acatctttca ttgaatgtac
     4741 ggcgatatca gcgcgattct ccaggagcgc gacttccagc tcttttacaa ataagccttt
     4801 tccgcctact ttcgccagcg gcgtatcaag aatcacatcg ccgcgcgtca ccatcggtac
     4861 cagttcaacg accaggcccg gatggctcgc catcaacttg tctttgacat agtgtgcctg
     4921 ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa acattgtcta acatgcttgt
     4981 taccgtcatt atcatccgtg gtccatccta acatccttgc cagagtgatg tcagtgttgt
     5041 ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc tggcagtgga tcctgacagg
     5101 cgtttcacgc cgtcgtaata aggaatttac agagaataaa cggtgctaca cttgtatgta
     5161 gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt gatcacgttt tagaccattt
     5221 tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt aacggttacc tttgacatac
     5281 gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg gaatcacagt catgacgggt
     5341 agcaaatcag gcgatacgtc ttgtacctct atattgagac tctgaaacag agactggatg
     5401 ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg
     5461 tctacagtct actgccgaca ttgttgcact atcaccatcc gcgaatgccg ggttaccttg
     5521 atggtaacgt tcccaaaggc atttgccttt acacgcctga tgaaactcaa cgccactacc
     5581 tgaacgagct tgaactgtat cgtggaatgt cagtacagga tccaccgaaa ggtgagcttc
     5641 caattactgg tgtatacacc atgggcagca cctcgtccgt agggcaaagt tgttcctctg
     5701 acctggatat ctgggtctgt catcaatcct ggctcgatag cgaagagcgc cagttactac
     5761 aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct gggtgtggaa gtcagcttct
     5821 tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt
     5881 gtggctccac ccagcatata ctgctgcttg acgaatttta tcgtactgcc gtgcgtctcg
     5941 ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga agaagagcat tacgacgact
     6001 atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg
     6061 gcttaagctc gctttctgct gaagagtact ttggtgccag cctttggcag ctctacaaga
     6121 gtatcgattc cccatacaaa gcggtgctga aaacactgct gctggaagcc tattcctggg
     6181 aatacccgaa cccacgtctg ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga
     6241 ttgtatcgtt tggcctcgat ccatactgca tgatgctgga gcgtgttact gaatacctga
     6301 cggcgattga agatttcacc cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt
     6361 gcgaaaaact cagccgtgaa cgcgcctgtg taggctggcg tcgcgcagtg ttgagccagt
     6421 tagtgagcga gtggggttgg gacgaagctc gtctggcaat gctcgataac cgtgctaact
     6481 ggaagattga tcaggtgcgt gaggcgcaca acgagctgct cgacgcgatg atgcagagct
     6541 accgtaatct gatccgcttt gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg
     6601 atatcggcgt actgacgcgt aaactgtatg ccgcgtttga agcattgcca ggtaaagtga
     6661 cgctggtaaa cccgcagatt tcacccgatc tctcggaacc gaatctgacc tttatttatg
     6721 tgccgccggg ccgggctaac cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg
     6781 agtcgatcat cagccatcag ccgctggaat ataaccgtta cctgaataaa ctggtggcgt
     6841 gggcatggtt taacggcctg ctgacctcgc gcacccgttt gtatattaaa ggtaacggca
     6901 ttgtcgattt gcctaagttg caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc
     6961 gcttacctgc accgacaccg aaggcgctct acagcccgtg tgagattcgc catctggcga
     7021 ttatcgttaa cctggaatat gacccgacag ctgcgttccg caatcaggtg gtgcattttg
     7081 atttccgtaa gctggatgtc ttcagctttg gcgagaatca aaattgcctg gtaggtagcg
     7141 ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac gctgcacttc aacggcgagc
     7201 aatcgatgat cgaagccctg aaaactattc tcggcaaaat gcatcaggac gccgcaccgc
     7261 cagatagcgt ggaagtcttc tgttatagcc agcatctgcg cggcttaatc cgtactcgcg
     7321 tgcagcaact ggtttctgag tgtattgaac tgcgcctttc cagcacccgc caggaaaccg
     7381 ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg cttgttcttc gaacgcctga
     7441 atgtatcggt acagaagctg gagaacgcca tcgagtttta tggcgctatt tcgcataaca
     7501 aactgcacgg cttgtcagtg caggttgaaa ccaatcacgt caaattaccg gcggtggtgg
     7561 acggctttgc cagcgaaggg atcatccagt tctttttcga agaaacgcaa gacgagaatg
     7621 gctttaatat ctacattctc gacgaaagca accgggttga ggtgtatcac cactgcgaag
     7681 gcagcaaaga ggagctggta cgtgacgtca gtcgct
//
LOCUS       DAJVPT010000151         7713 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVPT010000151 DAJVPT010000000
VERSION     DAJVPT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..843)
                     /locus_tag="OQM47_003581"
     CDS             complement(<1..843)
                     /locus_tag="OQM47_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1481595.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENI"
     gene            complement(880..1491)
                     /gene="hycB"
                     /locus_tag="OQM47_003582"
     CDS             complement(880..1491)
                     /gene="hycB"
                     /locus_tag="OQM47_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1481596.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(1616..2077)
                     /gene="hycA"
                     /locus_tag="OQM47_003583"
     CDS             complement(1616..2077)
                     /gene="hycA"
                     /locus_tag="OQM47_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1481597.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            2289..2639
                     /gene="hypA"
                     /locus_tag="OQM47_003584"
     CDS             2289..2639
                     /gene="hypA"
                     /locus_tag="OQM47_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1481598.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            2643..3515
                     /gene="hypB"
                     /locus_tag="OQM47_003585"
     CDS             2643..3515
                     /gene="hypB"
                     /locus_tag="OQM47_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1481599.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            3506..3778
                     /gene="hypC"
                     /locus_tag="OQM47_003586"
     CDS             3506..3778
                     /gene="hypC"
                     /locus_tag="OQM47_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1481600.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            3778..4899
                     /gene="hypD"
                     /locus_tag="OQM47_003587"
     CDS             3778..4899
                     /gene="hypD"
                     /locus_tag="OQM47_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1481601.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            4896..5906
                     /gene="hypE"
                     /locus_tag="OQM47_003588"
     CDS             4896..5906
                     /gene="hypE"
                     /locus_tag="OQM47_003588"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1481602.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            5980..>7713
                     /gene="flhA"
                     /locus_tag="OQM47_003589"
     CDS             5980..>7713
                     /gene="flhA"
                     /locus_tag="OQM47_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1481603.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNI"
BASE COUNT         1818 a         2076 c         2106 g         1713 t
ORIGIN      
        1 gatattttcc agggtgtggt aagccagcag gcgctggatg ttgtgttcca tcaacgcata
       61 cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc agcgcgatcc cccaccacag
      121 cggtgcatta ccgcccagca gtgacagggt taacatgccc agcaggccaa ttttcatgac
      181 taccgtagaa aacaacgcgg cagctggcgc agaggcgtta gcatgtgcct gcggcaccca
      241 gccgtgcagc ggaataatcc cagccagcag gccaaagcca atcacaccga gcagccagat
      301 atcggaaccg agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc
      361 ataacgctgc cacagcagcc agcaagcaat caccagcagc agagtgccaa gacgcctcag
      421 cgcaaaccac agtttgccct ctttgctgtt gctggtgagg aacaccgcac acaaggccat
      481 gacttcggcc attaccacga acatgccgag gttgctggcg atgacggcgc agacggcggc
      541 agccatcaac atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc
      601 aatgttatag aggctgacaa acagaccgca aaggccgagg gtaatcagcc aaatcgcgtt
      661 aagcggggag atttgcacat cgtagcttac cagcgacagc gcaccgctca cgccaaccgc
      721 gccagtcagt acagtgaagc ccgcggctgc cgtatacaga ctaccaaccg cgccgccaat
      781 tccggctatc cagccactta gcgctttttg aaaagagaaa tgccagaaca gcggcggcga
      841 caaaccatgc cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg
      901 agcctgctga aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg
      961 tttgctgacg cgggcgatat cggtgttatc caccagatgc agggctttag tcgggcacat
     1021 ccgcacacag gccggacctt gttcatcaaa gctacaaagg tcacatttga cggcgatcgc
     1081 gcgaatacct ggcacccagt caagcaatgt gctgacacgc gccggagcag gcggtgccgg
     1141 tggcgctttc ggggtattgg cgtttgccgg aatatccagc ggacggctgc cggaaaattc
     1201 aattgcgcca aacgggcagg cgatgccgca cagtttgcag cttacgcaca gactttcatt
     1261 caactgcacg gccccatcaa cgcgggtgat ggcgttaacc gggcagacca ccgcgcaggg
     1321 tgcatcttca cagtggtgac agagctgcgg cgcagattct ttttcattga gcatcactct
     1381 caggcgcggc attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca
     1441 ggtgtggcag ccgatacaga gcgtggagtc ggcaattaca aaacgattca ccaggcattc
     1501 ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat
     1561 cgacacgccc atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg
     1621 ctgccgggct ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatgg
     1681 cggtgtataa gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt
     1741 cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg
     1801 caatccgctg tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt
     1861 taaagccatc cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt
     1921 ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct
     1981 gaaccagcga attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga
     2041 tgtaatcggc tttctcgctt atttcccaaa tagtcattgt caggttactc gtttaacaga
     2101 gatgccagct ttaagcattt tttgtgccaa cttttaattt attgttatta aagagatttt
     2161 taagctaaag atgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat
     2221 tcaccgcagg gataatcaac actggcgcaa ttattgcttg tagctggcaa tagttaatgg
     2281 gaggcgatat gcacgaaata accctctgcc aacgggcact ggaattgatc gaacagcagg
     2341 ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct caaaattggc gcattttctt
     2401 gtgtcgaaac cagctctctt gccttttgtt ttgatctggt ttgccgcggc agcgtggcgg
     2461 aaggttgtaa actgcacctc gaagaacaag aggccgaatg ctggtgtgaa acatgccaac
     2521 agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc acagtgtcat ggtgacatgc
     2581 tacagattgt ggcagacgac ggtttacaga ttcggcggat agaaatagac caggagtgag
     2641 cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta tatcgagggt gatgaacata
     2701 accctcattc cgcgtttcgt agcgcgccat ttgccccggc ggcacgcccg cagatgaaaa
     2761 tcaccggcat taaagcgcct gaatttaccc ccagccagac tgaagaaggc gacctgcatt
     2821 acggtcatgg cgaagcgggc actcacacac cgggcatgag ccagcgtcgg atgctggaag
     2881 tcgaaattga cgtgctggac aaaaacaacc gtctggctga acgcaaccgc gcgcgctttg
     2941 ctgcccgcaa gcaactggtg ctcaacctgg tttccagccc tggttccggt aaaaccaccc
     3001 tgctgacgga aaccttaatg cgcctgaaag acagcgttcc gtgcgcggtt attgaaggcg
     3061 accagcaaac cgtgaacgat gccgcacgca ttcgcgctac cggtacacca gcgattcagg
     3121 tgaacaccgg taaaggctgc catcttgacg cacagatgat tgccgacgcc gcaccgcgtc
     3181 tgccactgga cgataacggt attctgttta tcgaaaacgt tggcaacctc gtatgcccgg
     3241 ccagcttcga tctcggtgaa aaacacaaag tagcggtgct ttccgttacc gaaggtgaag
     3301 acaaaccgct gaaatatccg catatgtttg ccgccgcctc gctgatgctg ctcaacaaag
     3361 ttgacctgtt gccgtatctc aactttgacg ttgagaagtg catcgcctgc gcccgcgaag
     3421 tcaatccaga aattgaaatc atccttattt ccgccaccag cggcgaaggg atggaccagt
     3481 ggctgaactg gctggagaca cagcgatgtg cataggcgtt cccggccaga tccgcaccat
     3541 tgacggcaac caggcgaaag tcgacgtctg cggcattcag cgcgatgtcg atttaacgtt
     3601 agtcggcagc tgcgatgaaa acggtcagcc gcgcgtgggc cagtgggtac tggttcacgt
     3661 tggctttgcc atgagcgtaa ttaatgaagc cgaagcacgc gacactctcg acgccttaca
     3721 aaacatgttt gacgttgagc cggatgtcgg cgcgctgttg tatggcgagg aaaaataatg
     3781 cgttttgttg atgaatatcg cgcgccggaa caggtgatgc agttaattga gcatctgcgc
     3841 gaacgtgctt cacatctctc ttacaccgcc gaacgccctc tgcggattat ggaagtgtgt
     3901 ggcggtcata cccacgccat ttttaaattc ggcctcgacc agttactgcc tgaaaacgtt
     3961 gagtttatcc acggtccggg ttgcccggtg tgcgtactgc cgatgggtag aatcgacacc
     4021 tgcgtggaga ttgccagcca tccggaagtc atcttctgta cctttggcga cgccatgcgc
     4081 gtaccaggga aacagggatc gctgttgcag gcaaaagcac gcggtgccga tgtgcgcatc
     4141 gtttactcgc cgatggatgc gttgaaactg gcgcaggaga atccaacccg caaagtggtg
     4201 ttcttcggct taggttttga aaccaccatg ccgaccaccg ccatcactct gcaacaggcg
     4261 aaagcgcgtg atgtgcagaa tttttacttc ttctgccagc acattacgct tatcccgacg
     4321 ctgcgcagtt tgctggaaca gccggataac ggtatcgacg cgttcctcgc gccgggccac
     4381 gttagtatgg ttatcggcac tgatgcctat aattttatcg ccagcgattt tcatcgtccg
     4441 ctggtggtcg ctggtttcga accccttgat ctactgcaag gcgtggtcat gctggtggag
     4501 cagaaaatag cggcccacag caaggtagag aatcagtatc gtcgggtggt accggatgcc
     4561 ggtaacctac tggcgcaaca ggcgattgcc gatgtgttct gtgtcaacgg cgacagcgaa
     4621 tggcgcggct taggcgtgat tgaatcttct ggcgtgcacc tgacgccgga ttatcaacga
     4681 ttcgatgccg aagcacattt ccgcccggca ccgcagcagg tctgcgatga cccgcgcgcg
     4741 cgttgtggtg aggtattaac gggcaaatgt aagccgcatc aatgcccgct gtttggtaac
     4801 acctgtaatc ctcaaaccgc atttggtgca ctgatggtct cctccgaagg agcgtgcgcc
     4861 gcgtggtatc agtatcgtca gcaggagagt gaagcgtgaa taatatccaa ctcgcccacg
     4921 gcagcggcgg ccaggcgatg cagcaattaa tcaacagcct gtttatggaa gcctttgcca
     4981 acccgtggct ggcagagcag gaagatcagg cacgtcttga tctggcgcag ctggtagcgg
     5041 aaggcgaccg tctggcgttc tccaccgaca gttacgtcat tgacccgctg ttctttcctg
     5101 gcggtaatat cggcaagctg gcgatttgcg gcaccgcgaa tgacgttgcg ggcagtggcg
     5161 ctattccgcg ctatctctcc tgtggcttta tcctcgaaga aggattgccg atggagacac
     5221 tgaaagccgt agtgaccagc atggcagaaa ccgcccgcac ggcaggcatt gccatcgtta
     5281 ctggcgatac taaagtggtg cagcgcggcg cggcagataa actgtttatc aacaccgctg
     5341 gcatgggcgc aattccggcg aatattcact ggggcgcaca gacgctaacc gcaggcgatg
     5401 tattgctggt tagcggtaca ctcggcgacc acggggcgac tatccttaac ctgcgtgagc
     5461 agctggggct ggatggcgaa ctggtcagcg actgcgcggt gctgacgccg cttattcaga
     5521 cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc cacccgtggt ggtgtaaacg
     5581 cggtggttca tgagttcgcg gcagcctgcg gttgtggtat tgaaatttct gaatcagcgc
     5641 tgccggttaa acctgccgtg cgcggcgttt gcgaattgct gggactggac gccctgaact
     5701 ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa cgcggcagag caagtgctgg
     5761 cagcgttaca ttcccatcca ctggggaaag acgcggcgct gattggtgaa gtggtggaac
     5821 gtaaaggtgt tcgtcttgcc ggtctgtatg gcgtgaaacg aaccctcgat ttaccacacg
     5881 ccgaaccgct tccgcgtata tgctaataaa attctaaatc tcctatagtt agtcaatgac
     5941 cttttgcacc gctttgcggt gctttcctgg aagaacaaaa tgtcatatac accgatgagt
     6001 gatctcggac agcaagggtt gttcgacatc actcggacac tattgcagca gcccgatctg
     6061 gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg
     6121 gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt attacgcgtc gcgtgaaaaa
     6181 gacaccccca ttaaatatga agacgaaact gttctggcac acggtccggt acgcagcatt
     6241 ttgtcgcgcc ctgatacgct gcattgcagt tacgaagaat tttgtgaaac ctggccgcag
     6301 ctggccgcag gtgggctata ccccaaattt ggtcactatt gcctgatgcc actggcggcg
     6361 ggagggcata tttttggtgg ctgtgaattt attcgttatg acgagcgtcc ctggagcgaa
     6421 aaagagttca atcgtctgca aacatttacg cagatcgttt ctgtcgtcac cgaacaaatc
     6481 cagagccgcg tcgtcaacaa tgtcgactat gagttgttat gccgggagcg cgataacttc
     6541 cgcatcctgg ttgccattac caacgcggtg ctttcccgcc tggatatgga cgaactggtc
     6601 agcgaagtcg ccaaagaaat ccattactat ttcgacattg acgatatcag cattgtctta
     6661 cgcagccacc gtaaaaacaa actcaacatc tactccactc actatcttga taaacaacat
     6721 cccgcccacg aacagagcga agtcgatgaa gccggaaccc tcaccgaacg cgtgttcaaa
     6781 agtaaagaga tgctgctgat caatctctac gagcgggacg atttagcccc ctatgaacgc
     6841 atgttgttcg acacctgggg caaccagatt caaaccttgt gcctgttacc gctgatgtct
     6901 ggcgacacca tgctgggcgt gctgaaactg gcgcaatgcg aagagaaagt gtttaccact
     6961 accaatctga atttgctgcg ccagattgcc gaacgtgtgg caatcgctgt cgataacgcc
     7021 ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg ttgatgaaaa cctcgccctg
     7081 accgagcagc tcaacaatgt tgatagtgaa tttggcgaga ttattggccg cagcgaagcc
     7141 atgtacagcg tgcttaaaca agttgaaatg gtggcgcaaa gtgacagtac cgtgctgatc
     7201 ctcggtgaaa ctggctcggg taaagagctg attgcccgtg cgatccataa tctcagtggg
     7261 cgtaataatc gccgcatggt caaaatgaac tgcgcggcga tgcctgccgg attgctggaa
     7321 agcgatctgt ttggtcatga gcgtggggct tttaccggtg ccaacgccca gcgcattggt
     7381 cgttttgaac tggcggataa aagctctctg ttcctcgacg aagtgggcga tatgccactg
     7441 gagttacagc cgaagttgct gcgtgtattg caggaacagg agtttgaacg tctcggcagc
     7501 aacaaaatca ttcagacgga cgtacgttta attgccgcga ctaaccgcga tctgaaaaaa
     7561 atggtcgccg accgagagtt ccgtagcgat ctctattacc gcctgaacgt attcccgatt
     7621 cacctgccgc cactacgcga gcgtccggaa gatattccgc tgctggcgaa agcctttacc
     7681 ttcaaaattg cccgtcgtct ggggcgcaat atc
//
LOCUS       DAJVPT010000152         7699 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVPT010000152 DAJVPT010000000
VERSION     DAJVPT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..153
                     /locus_tag="OQM47_003590"
     CDS             <1..153
                     /locus_tag="OQM47_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1481604.1"
                     /translation="MRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQ
                     KKEYQA"
     gene            435..716
                     /locus_tag="OQM47_003591"
                     /pseudo
     CDS             435..716
                     /locus_tag="OQM47_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(866..953)
                     /locus_tag="OQM47_003592"
     tRNA            complement(866..953)
                     /locus_tag="OQM47_003592"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(917..919),aa:Ser,seq:gga)
     gene            1188..2126
                     /gene="ghrA"
                     /locus_tag="OQM47_003593"
                     /pseudo
     CDS             1188..2126
                     /gene="ghrA"
                     /locus_tag="OQM47_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            2181..2918
                     /gene="ycdX"
                     /locus_tag="OQM47_003594"
     CDS             2181..2918
                     /gene="ycdX"
                     /locus_tag="OQM47_003594"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1481605.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2942..3496
                     /gene="ycdY"
                     /locus_tag="OQM47_003595"
     CDS             2942..3496
                     /gene="ycdY"
                     /locus_tag="OQM47_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1481606.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3598..4089
                     /locus_tag="OQM47_003596"
     CDS             3598..4089
                     /locus_tag="OQM47_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1481607.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4222..4928)
                     /locus_tag="OQM47_003597"
                     /pseudo
     CDS             complement(4222..4928)
                     /locus_tag="OQM47_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(4955..5371)
                     /gene="csgF"
                     /locus_tag="OQM47_003598"
     CDS             complement(4955..5371)
                     /gene="csgF"
                     /locus_tag="OQM47_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1481608.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5396..5785)
                     /gene="csgE"
                     /locus_tag="OQM47_003599"
     CDS             complement(5396..5785)
                     /gene="csgE"
                     /locus_tag="OQM47_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1481609.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(5790..6440)
                     /gene="csgD"
                     /locus_tag="OQM47_003600"
     CDS             complement(5790..6440)
                     /gene="csgD"
                     /locus_tag="OQM47_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1481610.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            7193..7648
                     /gene="csgB"
                     /locus_tag="OQM47_003601"
     CDS             7193..7648
                     /gene="csgB"
                     /locus_tag="OQM47_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1481611.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT         2100 a         1668 c         1837 g         2094 t
ORIGIN      
        1 atgcgacttg aacgcaagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttac ctgcgctctg gcaaataaac tggctcgaat tgcctgggca
      121 atcactactc agaaaaaaga atatcaggcc tgatatagat gctgatataa ctcaattagt
      181 ttattttaaa caataagata caagttacct ttctggtttt gcgaagactg attattgatg
      241 acatgaacgg caaatcgacc tgattaaaaa cctaacagat gaaatggctc actgaagccg
      301 aggggatttt taggattatc aggtgcggaa ctcatcgggg cgcgggcatc cttgcccaca
      361 gagacgccgg atagatttaa gcaagccaac cattaatcaa aaatcagtgt tgcaaaaacg
      421 ggggtgacca tagatttcat cttatcaccg gacaacaggc tgaccgactc gccgatctgg
      481 aaagaatgtt gcacctgttc agcggtaaac caatccccga caaccgggga aacatcaccc
      541 ttcgtctgga tgatcacatt cagtctgttc agggtaaaga gagctatgaa gatgagatgt
      601 tcagcatcag atactttaag aagggctccg cgcatatcac gttcagaaaa ccagaactgg
      661 ttgacagact gaatgatatt atcgcaaaac actatcctga tatgctggca gtatagcttt
      721 gccagtatgc tcaaagatcg gaaattgtcc gataagatat gatcgcgggg catataaacc
      781 ggtttcagat ggcatacccg atggcatact gtctggaagg aaaaataaaa aagcgcatgt
      841 taaacaataa catgcactta taatctggcg gtgagggggg gattcgaacc cccgatacgt
      901 tgccgtatac acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt
      961 cgttcctgtc ttgctggaac gggcgctaat ttagggaaat catgacctga ggtcaacaaa
     1021 ctttttgaaa aaatcgcgcg tttattcaaa cattcaatca atgtgtggtt ttaataagcg
     1081 aaatctgctt tttttgccac cgaccacgga tttgttatgc tggtggcctt tgtagatcat
     1141 aacgataagt gcgaataaat ttcgcacaac gcttttcggg agtcagtatg gatatcatct
     1201 tttatcaccc aacgttcgat acccaatggt ggattgaggc actgcgcaaa gctattcctc
     1261 aggcaagagt cagggcatgg aaaagcggag ataatgactc tgctgattat gttttagtct
     1321 ggcatcctcc tgttgaaatg ctggcagggc gcgatcttaa agcggtgttc gcactcgggg
     1381 ccggtgttga ttctattttg agcaagctac aggcacaccc tgaaatgctg aacccttctg
     1441 ttccactttt tcgcctggaa gataccggta tgggcgagca aatgcaggaa tatgctgtca
     1501 gtcaggtgct gcattggttt cgacgttttg acgattatcg catccagcaa aatagttcgc
     1561 attggcaacc gctgcctgaa tatcatcggg aagattttac catcggcatt ttgggcgcag
     1621 gcgtactggg ctgtaaagtt gctcagagtc tgcaaaccta gcgctttccg ctgcgttgct
     1681 ggagtcgaac ccgtaaatcg tggcctggcg tgcaaagctt tgccggacgg gaagaactgt
     1741 ctgcatttct gagccaatgt cgggtattga ttaatttgtt accgaatacc cctgaaaccg
     1801 tcggcattat taatcaacaa ttactcgaaa aattacctga tggcgcgtat ctcctcaacc
     1861 tggcgcgtgg tgttcatgtt gtggaagatg acctgctcgc ggcgctggat agcgggaaag
     1921 ttaaaggcgc aatgctggat gtttttaatc gtgaaccctt accgcctgaa agtccgctct
     1981 ggcaacatcc acgcgtgacg ataacaccac atgtcgccgc gattacccgt cccgctgaag
     2041 ctgtggagta catttctcgc actattgccc agctcgaaaa aggggagagg gtctgcgggc
     2101 aagtcgaccg cgcacgtggc tactaataaa gcatcaggat tcctgctatc cttggcggga
     2161 attgaataca ggagagagtt atgtatcccg tcgaccttca tatgcatacc gttgccagca
     2221 cacatgcata tagcacattg agtgattaca ttgcccaggc caaacaaaag ggcattaaac
     2281 tttttgcgat caccgatcat ggcccggata tggaagatgc gccgcatcac tggcacttca
     2341 ttaacatgcg tatctggccg cgagtggttg atggggtagg gatcctgcgc ggcattgaag
     2401 ctaacattaa aaatgttgat ggtgaaattg actgcagcgg taaaatgttt gactcgctgg
     2461 atctgattat tgccggtttt catgagccgg tttttgcgcc acatgacaaa gcgaccaata
     2521 cacaagcgat gatctccact atcgccagcg gcaatgtgca tataataagc catcccggaa
     2581 atccgaaata tgaaatagat gttaaagccg ttgctgaagc agccgcgaaa catcaggtgg
     2641 cgctggaaat caataattcc tcatttttac actcacgtaa gggcagtgaa gacaactgtc
     2701 gtgcggtagc tgcagcggta cgtgatgctg gaggttgggt ggcattaggc tcggattctc
     2761 acaccgcgtt taccatgggg gaatttgaag agtgtcttaa aatcctcgac gcggtagatt
     2821 ttccgctaga gcgcattttg aatgtttctc cgcgccgctt actgaacttc cttgaatctc
     2881 gcggtatggc accgattgcg gaatttgcag acctttaatt actagtaatg gaaatatatt
     2941 aatgaacgag ttttctatcc tctgtcgtgt gctgggttcg ctctattacc gccaaccgca
     3001 agatccttta ctggtgccgc tgtttactct gattcgtgag gggaaactgg ctgcgaactg
     3061 gccactggag caggatgagt tactgacgcg tttacagaaa agctgtgata tgacgcaagt
     3121 ctctgccgat tacaatgcgt tgtttatcgg cgatgaatgt gccgtgccgc catatcgtag
     3181 cgcatgggtt gaggacgcaa cggaagcgga agtgcgcgct tttctttccg aacgagggat
     3241 gctactggcg gatacgccag ccgatcacat cggcacattg ctgcttgcgg cttcctggct
     3301 ggaagatcag tcaacggaag atgagagcga agcactggaa acactgttca gtgagtatct
     3361 gttaccctgg tgtggtgcgt tcctcggcaa agtggaggcc catgcaacca cgcctttctg
     3421 gcgcaccatg gcaccgctaa cccgtgatgc cattagtgca atgtgggacg agctggaaga
     3481 agattctgaa gagtaaatgt gatcatcgtc actcataact gcaacagata atttgttgtt
     3541 gcataaaatg tgtgctcgat ctcattcatg gccgcgtttt ctgctatcat gcgcggcatg
     3601 aacatacttc tctctattgc aatcacaaca ggcattctct ccggtatctg gggatgggtg
     3661 gctgtttccc tgggcttact aagctgggcg ggcttcctgg gctgtacggc ctactttgct
     3721 tgcccgcaag gtgggctgaa agggctggcg atctccgctg caaccctgct tagtggcgtg
     3781 gtgtgggcga tggtcattat ttacggtagt gcactggcac cacatctgga aattctcggt
     3841 tatgtcataa ccggtattgt cgcgtttctg atgtgtattc aggccaaaca gctgctgctt
     3901 tcatttgttc cggggacgtt tataggcgca tgcgcgacgt ttgccgggca gggtgactgg
     3961 aaactggtgt taccttcgct ggcgcttggg ctggtatttg gttacgcaat gaaaaacagc
     4021 ggcctgtggc tggcggcgcg tagtgcaaag acggctcacc gtgagcagga aatcaaaaat
     4081 aaagcgtgag gggcactcac gctttcgctt aaacagtaaa tgccggatta ttaattccgg
     4141 catttttatc tgtcaggatt ccggtggaac cgacatatgg cggtatttca ccagaatgtc
     4201 attctgccgt tctgctttat tttacaaatc ccacagacca cggtcgatac catcattaat
     4261 caggaaaatg acccctgttt cgatagccga catcagacac agcataacgg gttcgtttga
     4321 ggtgtaaccc acttcccctt ccagcaagcg ctggtagtca ataaagcgga aaaccccggc
     4381 ctgaacttca taggaaagta tcgtcttact ggtgttcacc gaggagagga tctcaccggt
     4441 actcacattg acgacacgca ggttcacggc aatctgatcg agctggtatt gcgtgtctgc
     4501 accgatccca aaatatcttg ccccaacacc gcccgattta acgttgcttt cataaccgat
     4561 aatcgaacct tcaaccataa tatttgccgc cgttaaagat tgcagcggga ttcggttatt
     4621 aatggcaacc gtgccgtttt cttgtgccgc acgaataacc ttgcgctcgt taagcaggtt
     4681 ttgtaagccc tggcgctttg cggaacagca gtggagaagt tacttgccgg gtagggttta
     4741 aattgcccgg tttcgtcctg aatgttgtat accgaaacaa agatcttacc cgtcggcgct
     4801 ggcagatggg tcaaatcttt gtagctctga gcacgaggca ttaatgtcgg tctggcggct
     4861 tctttaggcg gggcggttaa gcatccgctc agtaacatga cggcaaccaa aagaaataag
     4921 cgctgcatga ttattttcct tatgaagctg gggcttaaaa atcggttgag ttattttgta
     4981 aacccgaaac ctggatggtc gaggtttgtc cggttttacg atctgtcacg ttcaactgca
     5041 attgaccatc gcggttagca atatcaacaa tataatcgtt ggtcaccatg cggcccggtt
     5101 taccggtatt aatattcgac agtagcccac ctaaaatttg tgactggatg gcctgagtaa
     5161 agttatctaa cgctgagggt gtttcaatac caaagtcatc gttatagctc ggatctttat
     5221 aagagttttg ggcctgagcg ctatttaata aaaaagcgcc attatttggg ttaccaccaa
     5281 agtttggatt acggaactgg aaagtcatgg ttccagccca acttaatggc gaaataagca
     5341 tgagtagaac tactgcatgt ttgacacgca ttgcagcctc cgaacaattt tttatttaga
     5401 attcatcatg cgccaaatcg tccgtactta ataacgcctg atttatctgg cgacgattta
     5461 gtgcttcttc agtttgaatc agtgcaaaga cgacagtttt ctcggagtct cttttcaacg
     5521 ggaataaaaa agtctggaaa ataacatcct gattgaccgt tattgtgatc cagcttcccc
     5581 atcgtgcact gggcctttca ttaatcgtta agttacccgt atagtcactt tcccatttat
     5641 cactaaaggc tcggtaaaaa tcatggccaa tagatgaaac agtatggtca gttaacaatc
     5701 ccgggacttc tacctcaacg gcgtgaagat tccctgcggc gaacagaaat tctgccgcca
     5761 caatccagcg taaataacgt ttcatggctt tatcgcctga ggttatcgtt tgcccaggaa
     5821 accgcttgtg tccggttttt tacggctatc ttcttgaaaa gattataaag atgcgtttta
     5881 accgtatttt cgctgatgaa caacgaacga gcgatctcgt tattagacgc gccgatacgc
     5941 agcttattca ggatctcttt ttcccgatga gtaaggaggg ctgattccgt gctgttataa
     6001 cgatagttac cagaatgtgt aatcaggtaa ctggcaagct tttgcgtaaa gtagcattcg
     6061 ccacgcagga cgccttgcaa cccattgaca acacgttctt gatcctccat ggcataaaaa
     6121 acgccgttga tatgaggcca gttttcaatg tcgcggtacg ggtaatcttc aggcgtattt
     6181 agcaacaata ttttgatatt gttgtttttc ctgctcaaag tatcctgcca ataatggata
     6241 agctttttat ccgcttccat catatccaga agaataatag agcctgaaga gatatcgtcc
     6301 agagaacgtt gaatattatg taattttcct gtaattgcta gcgattgttt aaggtgctgc
     6361 aagagagctg tcgcctgcaa agaaggttta gtgatcaaca ataatgtatg accatgaata
     6421 ctatggactt cattaaacat gatgaaaccc cactttttta ttgatcgcac acctgacagc
     6481 tgcctctaaa atagaagcac cagaagtact gacagatgtt gcactgctgt gtgtagtaat
     6541 aaatcagccc taaatgggta aaatataaaa ctaatggatt acatctgatt tcaatctagc
     6601 cattacaaat cttaaatcaa gtgttaaaca tgtaacaaaa tgtaactcat tatattaaaa
     6661 tgttaacatt aaggttttat taagtttaga aatgatagaa aagttgtaca tttggttttt
     6721 attgcacaat tttaaaaaat catacaaatg gtgctaactt actaataata catataaaaa
     6781 atatttcggt gtagtccttt cgtcatgtaa aacgtttttg ttttttctcc acacctccgt
     6841 ggacaatttt ttactgcaaa aagacgaggt ttgtcacggc ttgtgcgcaa gacatatcgc
     6901 agcaatcagc gacgggcaag aagaatgact gtctggtgat ttttgatagc ggaaaacgga
     6961 gatttaagag aaaacaaaat attttttgcg tagataacag cgtatttacg cgggttttaa
     7021 tactttggta tgaacaaaaa aagaaaaata caacgcgcgg gtgagttatt aaaaatattt
     7081 ccgcagacat actttccatc gtaacgcagc gttaacaaaa tacaggttgc gttaacaacc
     7141 aagttgaaat gatttaattt cttaaatgta cgaccaggtc cagggtgaca acatgaaaaa
     7201 caaattgtta tttatgatgt taacaatact gggtgcgcct gggattgcag ccgcagcagg
     7261 ttatgattta gctaattcag aatataactt cgcggtaaat gaattgagta agtcttcatt
     7321 taatcaggca gccataattg gtcaggctgg gactaataat agtgctcagt tacggcaggg
     7381 aggctcaaaa cttttggcgg ttgttgcgca agaaggtagt agcaatcggg caaagattga
     7441 ccagacagga gattataacc ttgcatatat tgatcaggcg ggcagtgcca acgatgccag
     7501 tatttcgcaa ggtgcttatg gtaatactgc gatgattatc cagaaaggtt ctggtaataa
     7561 agcaaatatt acacagtatg gtactcaaaa aacggcagtt gtagtgcaga gacagtcgca
     7621 aatggcaatt cgcgtgacac aacgttaatt accattcgac ttttaaatca atccgatggg
     7681 ggttttacat gaaactttt
//
LOCUS       DAJVPT010000153         7689 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVPT010000153 DAJVPT010000000
VERSION     DAJVPT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            117..248
                     /locus_tag="OQM47_003602"
     CDS             117..248
                     /locus_tag="OQM47_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1481612.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            508..1503
                     /gene="pgl"
                     /locus_tag="OQM47_003603"
     CDS             508..1503
                     /gene="pgl"
                     /locus_tag="OQM47_003603"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1481613.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1544..2497)
                     /locus_tag="OQM47_003604"
                     /pseudo
     CDS             complement(1544..2497)
                     /locus_tag="OQM47_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2681..3733
                     /locus_tag="OQM47_003605"
     CDS             2681..3733
                     /locus_tag="OQM47_003605"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1481614.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3809..5242
                     /locus_tag="OQM47_003606"
     CDS             3809..5242
                     /locus_tag="OQM47_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1481615.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5425..7605
                     /locus_tag="OQM47_003607"
     CDS             5425..7605
                     /locus_tag="OQM47_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1481616.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         1891 a         1759 c         1975 g         2064 t
ORIGIN      
        1 tctttaaaaa aacagtacat tctattttat aatgactgtg tattcccatg gaaggagatc
       61 acccaattct cccccccgac ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc
      121 ttattggcta tgtacgcgta tcaacaaatg accagaatac cgatctacaa cgtaatgcgt
      181 ttatttgtgc cggaagtgag ttgattgttg cgcgaatggg aagttggcca gaagttgtta
      241 tggcatagtg tttacggtcg ttgatacagg taaacaaaag cggggagagt catactccct
      301 gaatagtcgc gaaaatatct gtggcattgt caaattcgtg tcggtgacta atattttagt
      361 aatatgccat tcgaaatgga gtgctaactt attgatttaa ttgatgtgcg atattgtatt
      421 ttatcgtgtt ttgttgtctg ttttttatcc agtaagtgtt gttatatgta tgaaagataa
      481 gggatttatc atttttgagg tgtgttcatg aagcaaacag tttatatcgc cagccctgag
      541 agccagcaaa ttcacgtctg gaacctgaat catgaaggcg cactgacgct gacacaggtt
      601 gtcgatgtgc cggggcaggt gcagccgatg gtggtcagcc cggacaaacg ttatctctat
      661 gttggtgttc gccctgagtt tcgcgtcctg gcgtatcgta ttgccccgga cgatggcgca
      721 ctgacctttg ccgcagagtc tgtgctgccg ggtagtccga cgcatatttc caccgatcac
      781 caggggcagt ttgtctttgt aggttcttac aatgcgggta acgtgagcgt aacgcgtctg
      841 gaagatggcc tgccagtggg cgtcgtcgat gtagtcgagg ggctggacgg ttgccattcc
      901 gccaatatct caccggacaa ccgtacgctg tgggttccgg cattaaagca ggatcgcatt
      961 tgcctgttta cggtcagcga tgatggtcat ctggtggcgc aggaccctgc ggaagtgact
     1021 accgttgaag gggccggccc gcgtcatatg gtattccatc caaacgaaca atatgcgtat
     1081 tgcgtcaatg agttaaacag ctcagtggat gtctgggaac tgaaagatcc gcacggcaat
     1141 atcgaatgtg tccagacgct ggatatgatg ccggaaaact tctccgacac ccgttgggcg
     1201 gctgatattc atatcacccc ggatggtcgc catttatacg cctgcgaccg taccgccagt
     1261 ctgattaccg ttttcagcgt ttcggaagat ggcagcgtgt tgagtaaaga aggcttccag
     1321 ccaacggaaa cccagccgcg cggcttcaat gttgattaca gcggcaagta tctgattgcc
     1381 gctgggcaaa aatctcacca catctcggta tacgaaattg ttggcgagca ggggctactg
     1441 catgaaaaag gccgctatgc ggtcgggcag ggaccaatgt gggtggtggt taacgcacac
     1501 taaccgctga tttaaccggc gcagtctctc ctgcgccggt gtattaactt atctcctgta
     1561 acgcatgtct ctggcgttcg acgatattgg tccacaaatt gtctttatcg tcagtccaca
     1621 aattaatcag caaggaaaaa aagcgttcag ctgccggaga aagcacggca tctttgcggc
     1681 gaataatccc caatgtccga cgtataacgg gttccaccag tgggatacca ataatcgaag
     1741 aatagggcgc atggggcatt gccagaccag gaagcgccga aatccccagt cctgcctcca
     1801 ctagtcctaa tgacgtcgaa agatgacgca cttcgtaaaa ccagtccagc ttccagggct
     1861 tgtcggctag ctgttgttct atcaacaatc ggttgccgct ggaggaacgt acgccaatca
     1921 ttttgtaacc cactaactcc tgccattcta caagctactt tttggccaat ggatggtcac
     1981 gccgacaggc cagcacgaat ggctcgttga ccagtggggt aaaatcaatg gatgaatttg
     2041 tgacgttgtt catgtttatg ccaaagtctg attcgttaca cagcaccgat tccatgcaat
     2101 tattcgtgcc ttgttccaga atccgcactt taatattggg atacagctca ttaaatttac
     2161 cgattgccag cggtaaaaaa taaaatactg cggtcggaat acacgctaat gtcaccatac
     2221 cacgatgata agcattcata tcacgaatat taaaaagcgt ttcatcgaat ttctttatta
     2281 attcccgcgc ctccgggagc aacctttttc ctgcttttgt taacgtaacc ttgcgagttg
     2341 tacgctcaaa tagttgaata tgtaaatctt cctccatctt ttttattctt cgcgttaatg
     2401 caggttgcgt aatattgagt aatttagcgg cattgttaaa tgaactggac tctgccagta
     2461 tgacaaatgc cttcatactt gataattcat gcttcattat tactccggaa aatggaagcg
     2521 acgattttgg ggggctggtc gttaaaaatt ttaactgcat ttagccaact taaattaatg
     2581 aaaaaatgtt attaatcgtt gagctaaagt cattagagat gctttgtcct taatgtaacc
     2641 atatcgcaat aagttatgtt tttaaattga gggcattatt atgaaaaaaa taccctgcgt
     2701 gatgatgcga ggtggaacct cgaggggcgc gttcctgtta gcggaacatt tacccgaaga
     2761 tcaaacgcag cgcgataaaa tattgatggc aattatgggt tccggtaacg atctggaaat
     2821 tgacggtatt ggcggcggta atccactgac cagtaaagtc gccattatta gccgttccag
     2881 cgatccgcgt gctgatgtcg attatctgtt tgctcaagtt atcgtccatg agcaacgtgt
     2941 cgataccacg cctaactgcg gcaatatgct gtctggcgta ggggcattcg ccattgaaaa
     3001 tggtttgatt gcagcgactt cgccagttac ccgtgtacgt atccgcaacg tcaatacggg
     3061 tacgttcatc gaagctgatg tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc
     3121 cagaattgac ggcgtaccgg gtactgccgc accggttgcg ctcactttcc tgaatgccgc
     3181 tggaacgaaa actggaaaag ttttcccgac tgataatcag attgattatt ttgacgatgt
     3241 cccggtgacc tgtatcgata tggcgatgcc agtcgtcatt attccggctg aatatctggg
     3301 aaaaacaggt tatgaattac cggcggaact ggatgccgac aaagcattat tagcccgcat
     3361 tgaatctatc cggctacaag cgggtaaagc aatgggctta ggtgatgtca gtaatatggt
     3421 tatccctaaa cctgtgctta tttctccagc gcagaaaggc ggggcaatta atgtgcgtta
     3481 ttttatgccg cattcttgcc atcgcgcgct ggcgataacc ggtgctattg ctatttccag
     3541 tagttgtgca ttggaaggca ccgtcacccg acaaatcgtc ccttctgtag gatatggcaa
     3601 tatcaatatt gaacacccca gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga
     3661 tgccacgacg ttacgcgcat ctgttattcg gacaaccaga aaaatatttt ccggtgaagt
     3721 ttatcttccc tgaaaaaatt cgttgtcagg ataaggacaa tcaataaagg acttctgtat
     3781 gagtcataca gaaagaacag gattttaaat gaataagaaa tcgttatgga agctaattct
     3841 gatattagcg atcccatgta ttattggttt tatgccagct ccggcaggat taagcgaact
     3901 ggcgtgggtg ctttttggta tttacctggc ggccattgtg ggtctggtta tcaagccttt
     3961 cccggaacct gtcgtactgt taattgccgt tgctgcctca atggtggtgg tcggtaactt
     4021 atccgacggt gcgtttaaga ccaccgccgt attaagcggt tactcttcag gtactacctg
     4081 gctggtgttc tcggcgttta ccttaagcgc cgcatttgtg accaccggtt taggtaaact
     4141 tattgcctat ctgctgattg gtaaaatcgg taacaccatg ctgggtctgg gttacgttac
     4201 ggtattcctc gatctggtac tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg
     4261 cattgtgtta ccgatcatca acagcgtggc ggtggctttg gggtccgaac cggaaaaaag
     4321 tccgcgtcgt gtcggacatt acctgatgat gtccatttac atggtcacca aaaccaccag
     4381 ctatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga tcaacgacat
     4441 tctgcacctg caaattagct ggggtggatg ggcgctggcc gccggattgc cgggcatcat
     4501 tatgctgctg gtcaccccgc tggtaattta caccatgtat ccaccagaaa ttaaaaaggt
     4561 ggataacaaa accatcgcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga
     4621 aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc tggattttca gtaagtctct
     4681 gggggttgat gaatccactg tggcaatcgt tgttatggcg accatgctgc tgctgggtat
     4741 cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg
     4801 cggtattatc ggcttaagct ccttattatc gaaagttaaa tttttcgaat ggttagctga
     4861 agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat
     4921 tttcctcagc atcatcgtgc gttatttctt cgcttccggt agtgcctata tcgttgctat
     4981 gttacctgta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct
     5041 ggcactgttg ttctccaact cctatggcgg catggttact cactatggcg gcgcggcagg
     5101 tccggtcatc tttggcgtgg gttacaacga tattaaatcc tggtggttgg tcggtgcggt
     5161 actgacgata ttaaccttct tggtgcatat caccctcggc gtgtggtggt ggaatatgct
     5221 gatcggctgg aacatgctgt aaatatactc gtcatacttc aagttgcatg tgctgcgtct
     5281 gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact cacttgtcgc
     5341 cttcctgcaa ctcgaattat ttagagtata tccatttatt atctttctgc gcacttcacg
     5401 gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc
     5461 agtaataacg aaataattgc cgaagaacat ttcaccggcg aaattaaaaa agaagaagca
     5521 aaaaaaggca ctattgcctg gtctattctc tcttctcata atacgtccgg aaatatggat
     5581 aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt tggtattgta
     5641 cagaccgcta aagcgtccgg aatggaacgt ttcccgctgc cgtatgtgct gaccaactgc
     5701 cataactcac tctgcgccgt cggcggcacc attaacggtg atgaccatgt ttttggttta
     5761 tcggcagctc agcgttatgg cggtattttt gtgcctccgc atattgcggt catccatcaa
     5821 tatatgcgtg agatgatggc tggcgggggc aaaatgatcc tcgggtcaga cagccacacc
     5881 cgttacggtg cattagggac aatggcagtc ggtgagggtg gcggtgagtt ggtaaaacag
     5941 ctgcttaatg acacctggga tatcgactat ccgggcgtgg ttgcggtgca tctgaccgga
     6001 aaacctgcgc cgtatgtggg gccacaggat gtggcgctgg ctatcattgg cgcggtgttc
     6061 aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg gaccgggcgt tagcgcgctc
     6121 tctaccgatt tccgtaacag cgttgacgtg atgaccacgg aaaccacctg tttaagctcc
     6181 gtctggcaaa ctgatgaaga agtgcataac tggctggcgc tgcacggtcg cggtcaggat
     6241 tactgccagc ttaaccctca accgatggcg tactacgatg gctgcatcag cgttgattta
     6301 agcgccatca aaccaatgat tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac
     6361 acactgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc cgaacgcgtg
     6421 gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg cctgaaagtg
     6481 cagcagggga ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg
     6541 aatgcactgc gcggtcaatc ctgtggcaat gacaccttct cgctggcggt ttacccgtca
     6601 tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt gattggcgca
     6661 ggcgcaatca tcagaaccgc attctgtggc ccatgctttg gcgcgggcga tacgccaatc
     6721 aacaacggtt tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga aggctctaag
     6781 ccagccaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat cgctgcgact
     6841 gcggcaaacg gtggctattt aacctctgcc agcgaacttg attgctggga caacgtgccg
     6901 gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt atcagggctt tgtgaaaggg
     6961 gcaactcagc aaccgctgat ttacggaccg aacattaaag actggccgga attgggtgcg
     7021 ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc tcgacgaagt caccaccacc
     7081 gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat tggtctggcg
     7141 gggaatgtca gcgagctgac agaggtgttt gcgcgcatta agcagattgc tggtcaggag
     7201 catattgatc cgctgcaaac tgaaattggc agcatggtct atgcggtgaa accaggcgat
     7261 ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt
     7321 gccgaggagt acgcgactaa acgctaccgt tctaacgtca tcaactgggg gatgttaccg
     7381 ctgcagatgg cggaagtgcc aacctttgaa gtgggggatt acatttacat ccctggcatt
     7441 aaagcggcgc tggataatcc gggtacgacg tttaaaggtt atgtgatcca tgaagatgcg
     7501 ccggtaacgg aaattacgct ttacatggaa agcctgacgg ctgaagagcg cgagattatc
     7561 aaggcgggta gtttgattaa cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa
     7621 tgtaggtcgc attcggcact tattgtcgga tgcgatgctg gcgcatctta tccgacctac
     7681 aaatcgcat
//
LOCUS       DAJVPT010000154         7614 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVPT010000154 DAJVPT010000000
VERSION     DAJVPT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..821
                     /locus_tag="OQM47_003608"
                     /pseudo
     CDS             1..821
                     /locus_tag="OQM47_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            826..1791
                     /gene="rbsC"
                     /locus_tag="OQM47_003609"
     CDS             826..1791
                     /gene="rbsC"
                     /locus_tag="OQM47_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1481617.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1816..2706
                     /gene="rbsB"
                     /locus_tag="OQM47_003610"
                     /pseudo
     CDS             1816..2706
                     /gene="rbsB"
                     /locus_tag="OQM47_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2832..3761
                     /gene="rbsK"
                     /locus_tag="OQM47_003611"
     CDS             2832..3761
                     /gene="rbsK"
                     /locus_tag="OQM47_003611"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1481618.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3765..4757
                     /gene="rbsR"
                     /locus_tag="OQM47_003612"
     CDS             3765..4757
                     /gene="rbsR"
                     /locus_tag="OQM47_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1481619.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4723..6150)
                     /gene="mdtD"
                     /locus_tag="OQM47_003613"
     CDS             complement(4723..6150)
                     /gene="mdtD"
                     /locus_tag="OQM47_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1481620.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6173..6865)
                     /locus_tag="OQM47_003614"
     CDS             complement(6173..6865)
                     /locus_tag="OQM47_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1481621.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7353..>7614
                     /locus_tag="OQM47_003615"
     rRNA            7353..>7614
                     /locus_tag="OQM47_003615"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1945 a         1834 c         2017 g         1818 t
ORIGIN      
        1 accgaagatt cgctgattga gatgatggtg ggccgcaagc tggaagatca atatccacac
       61 ctggacaaag cgccaggaga tatccgcctg aaagtcgata atctctgcgg acctggcgtt
      121 aacgatgtct cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc
      181 gcaggtcgta ccgaactgat gaaagtgctc tacggcgcac tgccgcgcac cagcggttac
      241 gtcaccctgg atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaaacggc
      301 attgtgtata tctccgaaga ccgtaaacgt gacggtttag tgctgggcat gtcagtaaaa
      361 gagaacatgt cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat
      421 gccgatgaac agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacgccgtcg
      481 atggaacagg caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc
      541 ggtctgatga cacccccaaa gtattgatcc ttgatgagcc tacccgtggc gtagatgtcg
      601 gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc ttgagtatca
      661 ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc atcgtcatgc
      721 atgaagggca tctcagcggg gaatttactc gtgagcaggc cacccaggaa gtgttaatgg
      781 ctgtcgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgat aacccagact
      841 gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc gcttatcgct
      901 ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac catcaataac
      961 ttattcaata ttctccagca aacttcggtg aacgccatta tggcggtcgg gatgacgctg
     1021 gtgatcctga cgtcgggcat agacttgtcg gtaggttctc tgttggcgct aaccggcgca
     1081 gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc tgctgctctc
     1141 acgttaggtg ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag
     1201 gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc
     1261 aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg ctggtttggt
     1321 attggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt cttcctcgcg
     1381 gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct gggcggcaac
     1441 gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcaaaatcat cgtctattct
     1501 ctttgtggtc tgctggcatc gctggccggg atcattgaag tggcgcgtct ctcctctgca
     1561 caacccacgg cggggactgg ctatgagctg gatgctattg ctgcggtggt tctgggcggt
     1621 acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc attaattctt
     1681 ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca gatgatcgtc
     1741 aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta ataacgacta
     1801 caggacatct tgaatatgaa catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta
     1861 agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt ggtctccacg
     1921 cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc ggataaactt
     1981 ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct ggcgaacgtg
     2041 caggacttaa ccgttcgcgg caccaaaatt ctgctgatta acccgaccga ctccgacgca
     2101 gtgggtaatg ctgtgaagat ggctaactag gcgaacattc cggttatcac tcttgaccgc
     2161 caggcaacga aaggtgaagt ggtgagccac attgcatctg ataacgtact gggcggcaaa
     2221 atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa
     2281 ggcattgccg gtacatccgc agcccgtgaa cgtggcgaag gcttccagct ggccgttgct
     2341 gctcacaagt ttaatgttct tgccagccag ccagcagatt ttgatcgcac caaaggtttg
     2401 aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt cgcgcagaat
     2461 gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg
     2521 gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg caaactagca
     2581 gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa
     2641 gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact ggttgttaag
     2701 cagtagtttt aatcaggttg tatgacctga tggtgacata aatacgtcat cgacagatga
     2761 acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg cattttatgg
     2821 acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt aatgctgacc
     2881 acattcttaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt aaccactatc
     2941 aggttgcatt tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga
     3001 atatcgcgtt tattgcctgt actggcgatg acagcattgg cgagagcgtt cgccagcagc
     3061 tcgccactga taacatcgat attactccgg tcagcgtgat caaaggcgaa tcaacaggtg
     3121 tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat cggtattcat gccggcgcta
     3181 atgctgctct ttccccatcg ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag
     3241 cgttattaat gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc
     3301 atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt cctgacgaac
     3361 tgctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag ctcaccggta
     3421 ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat gaaaagggta
     3481 tccgtactgt actgattact ttaggaagtc gtggtgtatg ggctagcgtg aatggtgaag
     3541 gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc ggagatacct
     3601 ttaacggcgc gttaatcacg gcgttgctgg aagaaaaacc attgccagag gcgattcggt
     3661 ttgcccatgc tgccgctgcg attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt
     3721 ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct acaatgaaag
     3781 atgttgcccg tttggcgggc gtttctacct caacagtttc tcacgttatc aataaagatc
     3841 gcttcgtcag tgaagcgatt accgccaaag ttgaagcggc gattaaagaa ctcaattacg
     3901 cgccatcagc tctggcgcgt agcctcaaac tcaatcaaac acataccatt ggcatgttga
     3961 tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa cgcagctgct
     4021 tcgaacgcgg ttatagtctc gtcctttgca ataccgaagg cgatgaacag cggatgaatc
     4081 gcaatctgga aacgctgatg caaaaacgcg ttgatggctt actgttactg tgtaccgaaa
     4141 cgcatcagcc ttcgcgtgaa atcatgcaac gttatccgac agtgcctacc gtgatgatgg
     4201 actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg ctgggcggag
     4261 acttagcaac gcaatatctg atcgataaag gtcatacccg tatcgcctgt attaccggtc
     4321 cgctggataa aactccggcg cgcctgcggt tggaaggtta tcgggcggcg atgaaacgtg
     4381 cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt aacggcgggt
     4441 ttgacgccat gcgccaactg ttatcacatc cgctgcgtcc tcaggccgtc tttaccggaa
     4501 atgacgctat ggctgttggc gtttaccagg cgttatatca ggcagagtta caggttccgc
     4561 aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg acgccaccat
     4621 taaccactat ccaccaaccg aaagatgaac tgggggagct ggcgattgat gtactcatcc
     4681 atcggataac ccagccgacc cttcagcaac aacgattaca acttactccg attctgatgg
     4741 aacgcggttc ggcatagatt tacgctgtct tttgatcaaa ttattaccat cggttgtttt
     4801 cagaagcatg aacattgctg ctgaagcaac agtaataatg cccatcgtga taaacgtata
     4861 gtggaattgt tcgacagtcg ttgtgccttc catcccttca taaatgcgaa ggacggccgc
     4921 acttacagca acgcctaaac taatcgacag ttgctgcgtg accgccagaa cactgttacc
     4981 gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcatcg cggtaaactg
     5041 cgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata tcgccattgc
     5101 cggtgattgc aaagagaact gagcgatcat tagcccaata atcaccgtga tccccactaa
     5161 tgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg caataatgga
     5221 acctaacgct gtcggtgcca tcatacagcc ggcaataaac gcctgataac caaatcctac
     5281 ctgtaacatc aatggcataa ggaacggtac acagccggtc cccagacggg ttgcaatatt
     5341 gcctacgata ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt
     5401 tggcgtacgt cgcgcatgga gaatatagag aagcagtaac ccgatgctgg tgacaattac
     5461 cgtcaaggca atccagctgg cgacaatctt ttccccgaat agctctattc cgcttgaaaa
     5521 gagaacaagg ctgaggccaa acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt
     5581 ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta tggggatatt
     5641 aattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa gaacggggcc
     5701 taaaattggc cccaccagac ccggcatggc gacaaaattc aataccggaa gaagttcatt
     5761 acgaggataa gcacgcagta aggccggccg agcaacaggc atcatcattg cgtcgcctat
     5821 cccttgaata acccggaaga caaccagctg tggtagcgaa ttagaaagtg cgcaggccag
     5881 agaacccaac gtgaacagac tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc
     5941 ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac tgatgatggc
     6001 tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta aggcggtatt
     6061 cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc
     6121 cgacatacta cgcttctttt tatcgctcat tcaatgtcct gttatcgggt tatcacttat
     6181 caggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg ctttggataa
     6241 tcgcatcgac aatcgcctgg tgcagatcca gctttatcac tgtgtcgctg gtaattgacg
     6301 tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg
     6361 cgctcatttc atagatatgc tcatgccagg ccatatcgac ttcgatccag cgttcacggc
     6421 gaaagttctc ttttaatgcc gccatttcgg ccattaacgt attgagatgc gccttctgtt
     6481 ccgcggtgcc aaccgttgct gccagtaggc aggcttgcgg ctccagacag atacgcataa
     6541 ccagaaagtg atcgatgacc tgatgaaagt tctcttctgt tatccaccaa gtaagcaatt
     6601 cctgatcaag aaaattccag ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg
     6661 gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct gtacgactca
     6721 ctccaaattg ctcgcccagc tcaatctcac cgggcaaaat ggtgccgggt tcatattcac
     6781 cttttaagat ccgttgcgcc agcttctcag ccagaacata cgaaaggttt ttctgtgcag
     6841 ctaactgttg tgcgcttaaa ggcattactt atcttccttt ttctttttat tcctccttag
     6901 tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt tttatggcac
     6961 attaacgggg tgttttgctg aaaaaatgcg cggtcagaaa attattttaa atttcctctt
     7021 gtcaggccgg gataactccc tataatacgc caccactgac acggaacaac ggcaaatacg
     7081 ccgccgggtc agcggggttc tcctgagaat tccgacagaa aaagcaaaaa taaatgattg
     7141 actctgtagc gggaaagcgt attatgcaca cccccccccc cgcgccgctg agaaaaagcg
     7201 aagcgcactg ctctttaaca atttatcaga caatctgtgt gggcactcga agatacggat
     7261 tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa gagtgaacac gtaattcatt
     7321 acgaagttta attctttgag catcaaactt ttaaattgaa gagtttgatc atggctcaga
     7381 ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg gtaacaggaa gcagcttgct
     7441 gtttcgctga cgagtggcgg acgggtgagt aatgtctggg aaactgcctg atggaggggg
     7501 ataactactg gaaacggtag ctaataccgc ataacgtcgc aagaccaaag agggggacct
     7561 tcgggcctct tgccatcgga tgtgcccaga tgggattagc tagtaggtgg ggta
//
LOCUS       DAJVPT010000155         7569 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVPT010000155 DAJVPT010000000
VERSION     DAJVPT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1014)
                     /gene="rspA"
                     /locus_tag="OQM47_003616"
     CDS             complement(<1..1014)
                     /gene="rspA"
                     /locus_tag="OQM47_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1481622.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFG"
     gene            complement(1220..1546)
                     /locus_tag="OQM47_003617"
     CDS             complement(1220..1546)
                     /locus_tag="OQM47_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCS1481623.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1681..2022
                     /locus_tag="OQM47_003618"
     CDS             1681..2022
                     /locus_tag="OQM47_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1481624.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2088..2617
                     /gene="speG"
                     /locus_tag="OQM47_003619"
                     /pseudo
     CDS             2088..2617
                     /gene="speG"
                     /locus_tag="OQM47_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2620..3330)
                     /locus_tag="OQM47_003620"
     CDS             complement(2620..3330)
                     /locus_tag="OQM47_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1481625.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3438..3743
                     /locus_tag="OQM47_003621"
     CDS             3438..3743
                     /locus_tag="OQM47_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1481626.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3942..4262
                     /locus_tag="OQM47_003622"
     CDS             3942..4262
                     /locus_tag="OQM47_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCS1481627.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            4822..5400
                     /locus_tag="OQM47_003623"
                     /pseudo
     CDS             4822..5400
                     /locus_tag="OQM47_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6086..7075)
                     /locus_tag="OQM47_003624"
     CDS             complement(6086..7075)
                     /locus_tag="OQM47_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481628.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLSTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7160..>7569)
                     /locus_tag="OQM47_003625"
     CDS             complement(7160..>7569)
                     /locus_tag="OQM47_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481629.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYGRRQMNKRAEAERIAKEQRLQAERMREENMTDAEWQKELQRREQVKAERRT
                     YGESLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT         1981 a         1937 c         1831 g         1820 t
ORIGIN      
        1 accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa
       61 atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat cggcaatccg
      121 gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg cggatataat cgatgagttg
      181 ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca ctgcgatggg
      241 tgtgacggta tgttggcgaa tgagacggaa gcattcttgg ttttccgctg gcgtcgggtc
      301 ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg cttcaatagg
      361 cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact tgttacgtac
      421 cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc
      481 cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg acatgccgta
      541 ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc ccagctcctg
      601 atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat ggcaataaac
      661 catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt tggcagcttt
      721 ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg taaccggacc
      781 gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc
      841 gcgaccaata agccgcggac aaagatgatc ctgcaaataa gaggccacgg aaagctcacg
      901 tccattgagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga tttttaatgt
      961 gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct tcatgttcga
     1021 ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact gccatacaag
     1081 tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat
     1141 ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg tatcttattg
     1201 ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc aacattccgc
     1261 aaagcgcaat caacgcaccc gtccagtcat aaagacttag tttcacgcca tccacaacgc
     1321 gcagccacat caatgccgtg cagacataaa cgccaccata agccgcgtaa acacgcccac
     1381 tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc cccgccggaa
     1441 gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag catccaataa
     1501 tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt gtcacttatt
     1561 gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacatcc tcctggtacg
     1621 ttttgcgttc gcagtggatg gtgtagaatc acctttaatc attcatacag ggaatgaatt
     1681 atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc ttgcgcactg
     1741 gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc tggcgacagt
     1801 gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac gcgcaatctg
     1861 cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc cgcttttgat
     1921 aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc caatactttg
     1981 cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat tgatttaagg
     2041 aatgtaagga cacgttatgc caagcgccca cagttaagct acgcccgctg gagcgtgaag
     2101 atttacgcta tgtacatcaa ctcgacaata acgccagtgt gatgcgttac tggtttgagg
     2161 aaccctacga agcctttgtt gaactctctg atctgtatga taagcgcata ttcacgatca
     2221 gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt
     2281 ggaaattaac catgttcatc gccgggcaga atttcagata attatctccc cggagtatca
     2341 ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct tcaccgttct
     2401 caatctctat aagctgtatc tgatcgtcga taaagagaat gaaaaagcga ttcacattta
     2461 ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagtttttta ttaatggtca
     2521 atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggcag agcacaaaac
     2581 accgggtcag actctcctga agccgaccgc acaatagcat taataataat cgatcgtatt
     2641 tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgatcagctg
     2701 acagtccact ggattggcgt gactgtcata ttcacagctc tgttttacat taccaactcg
     2761 ttgattattt agtaaagtaa ccgctgtgta atctaatttt ttgatcggat ccgttgatgg
     2821 cgtggcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca gcggataccc
     2881 ctgatcatca tagcgatatt ccatctgcat ttgtttgctg ctggctttaa tcacgaagcc
     2941 attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct ggcattttcc
     3001 ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg taataattgg cgtccagtac
     3061 cagagcgacc acggtattat tttccagatc cagtaattcg agtgaatcaa aacagccttc
     3121 ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg
     3181 agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag agaaactggc
     3241 catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga ggaatatcgc
     3301 gagtaagcat ggtagtaatt tgtatttcac aacagtcacc agagagtaga gatgattctc
     3361 aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt
     3421 cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg
     3481 cctcacccgc ggtactcgcc gcgccgggat cttgtgagcg catacagagc gatatatcac
     3541 agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg
     3601 accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat acgcataaaa
     3661 ttctctacaa ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct acccaggatg
     3721 gcgcgcctgc cgaaccgcag taacacatta tcgactgaac gccggatatg acacatccgg
     3781 cgatttgaac atacaacata atctcacctt attactcata cccttctatt gatgtggatt
     3841 aataattctt aacccaaaat gggtagactc cctctattgt tagcgcgcta aatattcaat
     3901 gtataaacat ttatataaca ataaagaaca gggagtgagt tatgtccaaa aatgaccgaa
     3961 tggtgggcat cagccgcaga acactcgtta aatcaaccgc gacaggttct ctggcgctgg
     4021 ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc agcggcagcg gtacaacagg
     4081 ccagcgaaaa agtgatctgg ggttcctgtt ccgtcaactg tggtagccgc tgtgcacttc
     4141 gtctacatgt taaagataat gaagtgacct gggtggaaac tgacaatacc ggcagcgatg
     4201 agtacggcaa ccatcagggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     4261 aatcatcccg atcgcttgaa tgacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     4321 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     4381 gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat tgttggcggc
     4441 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     4501 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     4561 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     4621 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     4681 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4741 acggctgccg gtcgtgaaga ggaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4801 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4861 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4921 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4981 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     5041 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     5101 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     5161 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     5221 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     5281 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     5341 ttcatctgga actacaagga cttgaagcgg acccttagta ggcctgaggt cctgcattga
     5401 gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga tgtctagacg
     5461 gctggtcagg accctccggt ggcctcggtc tggacttgag gcgggctatg ggggaagtct
     5521 gctggcttaa tgtattagat ggtcattcac atttttctgt cagaaaccat cggtcccact
     5581 gtgagtacaa acctaagccc ccagaagccc acccacagcc ttagacagag tgtcgaatac
     5641 attcacaccg agtcgaccgg agaggaccat atagaggccc acagagagga acacgagttt
     5701 atagaccaga gtcaggaggg accttaggtc ttcggaagtc atggacataa agcccccccc
     5761 tatcttctat tgcactaccg cactagacaa ctagtacaaa acaaaggccc agaccattgg
     5821 tccagaccgt agttagttaa tcacagtgac aagccaccac ctgaagtcct gctgatattc
     5881 agtccagact tacctctgct catggtctta cctccctcgg tctcctgacc ttgggtatcg
     5941 actacagctt gctgagcgtc atctggagga gctacaggag gagctacagg aggcgggacc
     6001 gaagccccac cttgagtccc accaccacct gtcaggccct tggcgatgga cctgaagcct
     6061 ttactaatgc tcttacccat tggtcttacg cttcggtctt ggtgaacttc aggcgtttga
     6121 ctacaggcag gacggcagta gctgagccag tgcccatctt gatggtcgta gcagcttcag
     6181 cgacaacgaa cactttaccg aggatttcca agtcgtggga ctgagagtcg ccatcacgtg
     6241 aaaggacgac cgtaatattc tcaccgtcgt gacggaagca gccgaagctc agggccgtga
     6301 agcgtttagc cagcatgaat acacccttgt cttcaccgtt gtaataggcg atgtctgtcc
     6361 ccagcatatc gacgatagcg tcttcaccgg agaagccttg cttagcagcc agacgggaca
     6421 gtgctttcag ttggctctca tgagcgataa taccgtagtc agacacctca gaaccgaaag
     6481 aagactctgt gttctcatac agagcgtcca tgatagcgtc tgccttagcg gtcaggctac
     6541 cagtcaggac aggaagctct acggtcttca gcagcttaga ggattccaga acgtcgacca
     6601 tgcgagcgtt gatggtacgg accacattct cacagatgtt catagcgtcc tgctgaagtg
     6661 tgccctgagt gaatccggtg gatgacttgg cgtcccagag cttggtctgc tcagagatga
     6721 gaatagcctg ctcgattggt tcgaccacga tttcttgtcc agacggaacg gtcacgactt
     6781 caggtcgctt gccaccacgg acaggaacga ccacagagcg gtcagccaga gtgtgagcaa
     6841 tgattggggt cggtaagctg ttacgggtcg gcaggacttc tacagggaac gggatgttct
     6901 cttgagcgtc tttaagtaca ccgttcagca tagccttaac cgtcttagct aactggaggg
     6961 catagacctg cgggtcagcg ttagtcgaca gaccttcatt gacctgaatg ttgtcggtat
     7021 cggtgatgcg tactggctca atgccaaact ggaaagcgtc tttaatcgta gtcataataa
     7081 tcaatctcct aaattagttt aaatactgtg agtcctcaaa actgaggagt ccctaacatc
     7141 gggtcaggga ctgtgtgttt tacaagctgt ccatccagtt accgcctgac tcaaggtcag
     7201 cgacaatagc ggcacgggag cgaccagcgg aatgggtagc actgcggagg ctctcaccat
     7261 aggtccggcg ttcagctttg acctgttcgc ggcgctgtag ctctttctgc cactctgcat
     7321 cggtcatgtt ctcttcacgc atacgctcag cctgaagtcg ttgctcttta gcgatacgct
     7381 cagcttcagc tcgtttgttc atctggcggc gaccataggc ggtcatcaag tcattactga
     7441 agaagttgcc aatggtcacg ccagcttcag ccagagcttc cagtaactcc attgcctcgc
     7501 ggctggtaga cggcaggcct gacaggtagc ccggaatgag ggtcttgata gcgagcgaca
     7561 gcggatagg
//
LOCUS       DAJVPT010000156         7359 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVPT010000156 DAJVPT010000000
VERSION     DAJVPT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(76..696)
                     /gene="entD"
                     /locus_tag="OQM47_003626"
     CDS             complement(76..696)
                     /gene="entD"
                     /locus_tag="OQM47_003626"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1481630.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(871..3111)
                     /gene="fepA"
                     /locus_tag="OQM47_003627"
     CDS             complement(871..3111)
                     /gene="fepA"
                     /locus_tag="OQM47_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1481631.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3354..4556
                     /gene="fes"
                     /locus_tag="OQM47_003628"
     CDS             3354..4556
                     /gene="fes"
                     /locus_tag="OQM47_003628"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1481632.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4559..4777
                     /gene="ybdZ"
                     /locus_tag="OQM47_003629"
     CDS             4559..4777
                     /gene="ybdZ"
                     /locus_tag="OQM47_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1481633.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4774..>7359
                     /locus_tag="OQM47_003630"
     CDS             4774..>7359
                     /locus_tag="OQM47_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCS1481634.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDD"
BASE COUNT         1592 a         1979 c         1992 g         1796 t
ORIGIN      
        1 gtggtaaggc acctgcttta cactttcgcc cgcagtcagt gatggctgcg ggcgaatcgt
       61 accagatgtt gtcaattaat cgtgttggca cagagttatg actatttttt cttttatctg
      121 ccagtgcaca gcaaacatct cattctcacg atgaatgatg acctgctgtt tattccagct
      181 aattatctga tagtccagaa aacctgcatc agtttggatc tcacttgcct taaatgcgct
      241 ctctttggcg gaaaatgcaa gtgtcagcgc cagagaaaag gctaaaccgc agtctgcgag
      301 tcgctcgtgt tctgctggtg taataatgtt gtctgtcaat tcggtcgctg tttgcgcaga
      361 aaaaatttct tcgatatcaa cgccaatcgg ttgacgggaa accacggcca atgccgtagc
      421 cccgcagtgg ctaatactgc cgtatacctc cgcaggccac accggttggc gtagctcgcc
      481 gattgcgggc acacatttat agccatattc ccgcaaggca taaacagcag cgatccgtcc
      541 ggctaaatgc tctgttttac gtttacgtcc agcgtgttgc agttgtgcgt agtgcggcag
      601 ccagagtaaa tcctgctcac aaaaattcgc cggatcgaac tcaacaaaat gcagcgtatg
      661 tccggcaaag gggagggagg tatgcgtagt tttcatatcg accatattcg agactgatga
      721 caaacgcaaa actgcctgat gcgctacgct tatcaggcct acgtgatcct gcaatatatt
      781 gaattggcaa gatctttgta ggccggataa ggcgttcacg ccgcatccgg cgtgaacgac
      841 gcgcactttg tcaacaatct gacgttagca tcagaagtga gtattaatgc tcatatacca
      901 cgtacgtccc ggctcgttat aggtatacgc gccggcaccg gcgatatagt tggctcctgc
      961 cagatcgccc gtggtctggg cattacctgc acgccacaaa cgtttgtcga acaggttgtc
     1021 cacgccgccg gtcagactga cattcttcgt cacatcccag gtcgcgctca gacccacaat
     1081 gctgtaagga ctgatttctt tggtttccgg gccaaccgct ggctgacctt tatagttgta
     1141 cttcttcggc tgctgcttgc cgtaccaggt gaaggtcgtt tgcatcgaca aatcttcccg
     1201 tgcctgccag ctcagcgttg agttcaacgt gtactccggg atgatcgaca aacggtcgcc
     1261 cgtggttttg ttttcactct tcagcatata agtgatgtta ttggtccaca tcaccgtttc
     1321 gctaactggt acgtttaacg atccttccag accttcaacc actgctttcg gcacgttatc
     1381 ccactgatag agatcagtgc cgactgcgtt ttgccctaca gccacatagc ccgcttcaat
     1441 cttattgcga taatcgttac ggaaccaggt gacgcccgcc agccagccgt cgcgtttgaa
     1501 ctccagacca atctctttgt tgatgctggt ttctactttc aggtcatcgt taccttgcag
     1561 atagcagccg cccgcgctgg catagcagcc ctgaccttta ctgtagagaa tgtagttcgg
     1621 gttagtctgg tataggctcg gcgctttata ggcgcgggca atgcctattt tcagcgtgaa
     1681 gtcatcgcct aaaccttgcg atatgttcag cgccgggctc cagttattgc cgacaatact
     1741 gtgatgatcg aaacgcagcc ccggcgttac gatggtgctg tcagtcagct ccatgttgtt
     1801 ttcggcaaac agcgagaaaa tttctgcttt tgaatacggg ctacggtcgg tggcactcac
     1861 gccatcaata gcgccaccgg tattcgttcc ggtcagtgct tgggtgttgg aacttaagtc
     1921 cttcatccgt tgctgattcc actccgtacc cagcgtcagc gtctggttaa cgaggaaatc
     1981 aatcggcagg ttaacttcgc tgtgcagcat cacgtcatcg agatcgatat cgacgaaatc
     2041 ctgtgtcgct ttttcgttaa atttcccttc ggtaccgccc gccagacctt ccggaatacg
     2101 cgagttacgg gtgtgttcat actgcaccca gttgctggtg gtcacgccgt tatcccagcc
     2161 accgttccag gtcagcgcgt agttctggcg atacaggcgg ttggtttcat cgccatattt
     2221 cgagcgagtg taagcgtcag agttggtgtt ctgggtatcg cccgcataca ggttaccctg
     2281 gcggctgtaa cccgcttcca gttccagcga ttgcaatggt gcgaagtccc agcgcaccac
     2341 gccgttaata tctttattga tcaccccttc gcgcccggct ggtaacgtcg tggcatacgt
     2401 tccggcacgc gcggactgat ggccctggtt gatatcccac gcgtcagcct gagttttgtc
     2461 gaggttgcca tacaagcgga agctgaattc gtcgcccagc ggaccggtca ggctaaagtt
     2521 agtgcgtttg gtggcacctt cctctttatg ttctggcgca ttgaaatatg cgtcccagga
     2581 gccgtgccac tcgccgctgc cttttttggt aatgatgtta accacgccgc ccgccgcgcc
     2641 gttgccataa cgcgcagctg ccggaccacg cagaacttca atacgttcaa tcatttcagg
     2701 tggcacccag gaggtatcac cacgggtatc gcgctcgcca cgccagccct gacgcaccga
     2761 gttacggctg cttaccggct tgccgtcaat caaaatcagc gtgttttccg gacccatacc
     2821 gcgaatatca atctgtcggt tattcccacg ctgaccactg gtggagttac cggtcaggtt
     2881 aacgcctggc atggtacgga tgatctccga cacatcgcgg gcaaccgggt ttttgcggat
     2941 ttcatctgcg gtgatggtcg aaacgccagg agcctgcaag ttctgctcgg cggcggtaac
     3001 gacaatggta tcgtcatgtg aaacaggtgt atcggtcggc tcttgtgcct gcgctacccc
     3061 ataaatcccc agattgacca acaaggccag ggaatgaatc ttcttgttca ttgttttatt
     3121 cctgcatttt tgccacgaat tgcaactgtc gggcatggtc gtcatcaaca cgacgcatcc
     3181 cgttaccgcg aaaacctttg atcctgaaag acacgcagtg cagttggtta attaatgtcc
     3241 gcgcttccca cggcgcgcca ttacgctatt gcaaatgcaa atagttatta ataacattat
     3301 caatatattt ctgcaatcaa tgaaaaattg cacagtaaac atggggttat ggtgtgacgg
     3361 cgttaaaagt aggaagtgag agctggtggc agtcgaaaca tggcccggaa tggcagcgtc
     3421 tgaatgacga aatgtttgag gtcactttct ggtggcgtga tccccaaggt tctgaagaat
     3481 actcgacgat aaagcgcgta tgggtctaca tcactggtgt gaccgatcat catcagaaca
     3541 gccagcccca gtcgatgcag cgaattgcag gtactgatgt ctggcagtgg acgacacaac
     3601 tcaatgccaa ctggcgcggc agctactgct ttattcccac cgaacgcgat gacatttttt
     3661 ctgcaccatc ccccgatcgc ctcgaattac gtgaaggctg gcgaaaacta ttaccccagg
     3721 caatagccga tccgctgaat ccacaaagct ggaaaggcgg gctagggcac gctgtttctg
     3781 cactcgaaat gccgcaagcg cctctgcaac cgggatggga ttgtccgcaa gcgccagaaa
     3841 taccagccaa agaaattatc tggaaaagtg aacggttgaa aaattcacgt cgtgtatgga
     3901 tttttaccac cggcgatgta acagcagaag aacgcccgct ggcagttttg ctcgatggcg
     3961 aattttgggc gcaaagtatg cccgtctggc ctgtgctgac ttcgctgacc catcgtcagc
     4021 aacttcctcc cgccgtgtat gtgttgatcg acgctatcga caccacgcac cgcgcccacg
     4081 aactgccgtg taatgcggat ttctggctgg cagtacagca agagttatta cccctggtga
     4141 aagttattgc cccttttagc gatcgcgccg atcgcaccgt ggtcgccggg cagagttttg
     4201 gtgggctgtc cgcgctgtat gccggactgc actggcctga acgctttggc tgtgtattaa
     4261 gccagtcagg atcgtactgg tggccgcatc ggggcgggca gcaagagggc gtgttacttg
     4321 aaaagctaaa agctggtgaa gttagcgccg aaggtctgcg cattgtgctg gaagcgggta
     4381 ttcgcgagcc gatgatcatg cgggccaatc aggcgctgta tgcgcaatta caccctataa
     4441 aagaatccat tttctggcgt caggttgacg gcggacatga tgcgctttgt tggcgcggtg
     4501 gcttgatgca ggggttaatc gacctctggc aaccactttt ccatgacagg agttgaatat
     4561 ggcattcagt aatcccttcg atgatccaca gggagcgttt tacattttgc gcaatgcgca
     4621 ggggcaattc agtctgtggc cgcaacaatg cgccttaccg gcaggctggg acattgtgtg
     4681 tcagccgcag tcacaagagt cctgccagca gtggcttgaa gcccactggc gtactctgac
     4741 accggcgaat ttcacccagt tgcaggaggc acaatgagcc agcatttacc tttggtcgcc
     4801 gcacagcccg gcatctggat ggcagaaaaa ctgtcggatt taccctccgc ctggagcgtg
     4861 gcgcattacg ttgagttaac cggagaggtt gatgcgccat tactggcccg cgcggtggtt
     4921 gccggattag cgcaagcaga tactttgcgg atgcgtttta cggaagataa cggcgaagtc
     4981 tggcaatggg tcgatgatgc gctgatattc gaactgccag aaattatcga cctgcgaacc
     5041 aatattgatc cgcacggtac tgcgcaggca ttaatgctgg cggatttgca acaagatttg
     5101 cgcgtcgata gcggtaaacc actggtcttt catcaactga tacaggtggc agataaccgc
     5161 tggtactggt atcagcgtta tcaccattta ctggtcgatg gcttcagttt tccggccatt
     5221 acccgccaga tcgccaacat ttactgcgca ttgctgcgtg gcgaaccaac gcctgcttcg
     5281 ccgtttacgc ctttcgctga tgtagtggaa gagtaccagc aataccgcga aagcgaagcc
     5341 tggcagcgtg atgcggcatt ctgggtggaa cagcgtcgcc aactgccacc gcccgcgtca
     5401 ctttctccgg cacctttagc ggggcgcagc gcctcggcag atattttgcg tctgaaaatg
     5461 gaatttaccg acggggaatt ccgccagctg gctacgcaac tttcaggtgt gcagcgtacc
     5521 gatttagccc ttgcgctgac ggctttgtgg ctggggcgat tgtgcaatcg catggactac
     5581 gccgccggat ttatctttat gcgtcgactg ggctcggcgg cgctgacggc taccggaccc
     5641 gtgctcaacg ttttgccgct gggtattcat attgcggcgc aagaaacgct gccggaactg
     5701 gcaacccgac tggcaacccg actggcagca caactgaaaa aaatgcgtcg tcatcaacgt
     5761 tacgatgccg aacaaattgt ccgtgacagc gggcgagcgg caggtgatga accgctgttt
     5821 ggtccggtac tcaatattaa ggtatttgat taccaactgg atattcctgg tgttcaggcg
     5881 caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct gttcccggat
     5941 gaacacggtg atttgagtat tgagatcctc gccaataaac agcgttacga tgagccaacg
     6001 ttaatccagc atgctgaacg cctgaaaatg ctgattgccc agttcgccgc ggatccggcg
     6061 ctgttgtgcg gcgatgtcga tattatgctg ccaggtgagt atgcgcagct ggcgcagatc
     6121 aacgccactc aggttgagat tccagaaacc acgctgagcg cgctggtggc agaacaagcg
     6181 gcaaaaacac cggatgctcc ggcgctggca gatgcgcgtt acctgttcag ctatcgggaa
     6241 atgcacgagc aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt taaaccaggg
     6301 gacagcgtgg cggtggcact accgcgctcg gtctttttga ccctggcact ccatgcaata
     6361 gttgaagctg gcgcggcctg gctaccgctg gataccggct atccggacga tcgcctgaaa
     6421 atgatgctgg aagatgcgcg tccgtcgctg ttaattacca ccgacgatca actgccgcgc
     6481 tttagcgatg ttcccaattt aaccaacctt tgctataacg ccccgcttac cccccagggc
     6541 agtgcgccgc tgcaactttc acaaccgcac cacacggctt atatcatctt tacctctggc
     6601 tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgtcaa ccgcctgttg
     6661 tggattcaaa atcattatcc gctcacaggc gaagatgtcg ttgcccaaaa aacgccgtgc
     6721 agttttgatg tctcggtgtg ggagtttttc tggccgttta ttgccggggc taaactggtg
     6781 atggctgaac cggaagcgca ccgcgacccg ctcgctatgc agcaattctt tgccgaatat
     6841 ggcgtaacga ccacgcactt tgtgccgtcg atgctggcag catttgttgc ctcgctgact
     6901 ccgcaaaccg ctcgccagag ttgcgtgacg ttgaaacagg ttttctgtag tggtgaggcc
     6961 ttaccggctg atttatgccg cgaatggcaa cagttaaccg gcgcgccgtt gcataacctg
     7021 tatggcccga cggaagcggc ggtagatgtc agctggtatc cggcttttgg cgaggaactg
     7081 gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tatggaatac gggtctgcgt
     7141 attcttgatg cgatgatgca tccggtgccg ccgggtgtgg cgggtgatct ctatctcacc
     7201 ggtattcaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag ccgctttatt
     7261 gccgatcctt ttgctcctgg tgaacggatg tatcgtaccg gagacgttgc ccgctggctg
     7321 gataacggcg cggtggagta cctcgggcgc agtgacgat
//
LOCUS       DAJVPT010000157         7236 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVPT010000157 DAJVPT010000000
VERSION     DAJVPT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..234
                     /locus_tag="OQM47_003631"
     CDS             <1..234
                     /locus_tag="OQM47_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1481635.1"
                     /translation="IAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVD
                     PESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
     gene            complement(637..1218)
                     /locus_tag="OQM47_003632"
     CDS             complement(637..1218)
                     /locus_tag="OQM47_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481636.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(1265..5122)
                     /gene="sigA"
                     /locus_tag="OQM47_003633"
     CDS             complement(1265..5122)
                     /gene="sigA"
                     /locus_tag="OQM47_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1481637.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5483..6487)
                     /locus_tag="OQM47_003634"
                     /pseudo
     CDS             complement(5483..6487)
                     /locus_tag="OQM47_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6472..6849
                     /locus_tag="OQM47_003635"
                     /pseudo
     CDS             6472..6849
                     /locus_tag="OQM47_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6888..>7236)
                     /locus_tag="OQM47_003636"
     CDS             complement(6888..>7236)
                     /locus_tag="OQM47_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1481638.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1791 a         1645 c         1650 g         2150 t
ORIGIN      
        1 atcgctgaaa atgccttgcg ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc
       61 tctgaccatg gtggtgagcg gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg
      121 aatgacgtgg atccagaaag ctaccttcgc catgtgcttg gcgtcatagc agactggccg
      181 gtcaaccggg tcagcgaact gcttccgtgg cgcatagcac tgccagctga ataacacatc
      241 cccgtcaata cggccctcgc tgtacgctta ctcatcacct gctattaatc cccgagtcag
      301 tacttcgcgg cctgacgcga tcaccctact cagtacttca gagcccgcct ccggtacctg
      361 cctggcgatc gcccctgtgc aacaaaataa atcaaccgtt atcaaaccac gatcgttcgt
      421 atgcgccgcc gatgacgcgc gcctgaaaaa cgcgtcaata tgagttcccc ggaagcttac
      481 gataatacgc ttatgaacat tacaacacgc tcatgcggaa cctggatggg ggaaggaacg
      541 gtgaagctgt tagcgaatga ccggcgttta tacaaacgcc ggtcatgatg aatgatattt
      601 cagaatatac ctcgggctaa aataccggga cagagatcag gcgtgattaa tctgcagcat
      661 ttttaataac agaagtactg agcttacttt acctttgatg atgtaatagt tatttccgtc
      721 ggtactcatt tttccctgaa ataatgtcac ggatgtttct tcctgctgat cctgcaggcg
      781 cagtcgtaag gtcatatttt taatgagctg agatccgggt ggcaaggggt atttgctgtc
      841 agatactggg cgactgagtc tgatgtggcg cgtatacagc ccgttttcgt acagggtggc
      901 ctctgttatg gtgtcagtga ccagcatgtg ggagacaatg ctgtattctt ttttaaagca
      961 cccaccagaa acggtgattg taatgataat ggtcttctcg atcgagatgt aaatacccgt
     1021 ttcgttatac tgtccgtatc ggaaccatga gaactcagtt gcattccagc catgctccag
     1081 aatatgtgca tttttttgcc tgatagtttc tttttttatt cgctcatgtt taaggcataa
     1141 caggcagata aataatgtca gaaaaagaaa gctaataatg agagcaagag cgatatagtc
     1201 tgtcacaggg taatacatat tctctccggt agaagaaggg ccgcaaacgc ggcccgggct
     1261 gttatcagaa agagtaacgg aagttggcgt tgatcgcgtt atccacgttg tatttaccaa
     1321 atgccgattt ctcaaactca agaccgaagc gaagattatc gcgaatttcg gcgttgagac
     1381 caacattcat gagaatacga ccgtcttttt cacctttgat acgtttctca ccggacgcat
     1441 cacgcagtac ggtttcaccg ttggcaaaca ggtcaaactg gtagccaagg ccggcgcggg
     1501 ctgtgacttt ccagtcctta ccggagaagg atttacccac atcaacaccg gtacgcccaa
     1561 tcagcggatt aaagtcctta tccttcatgg tcacgctcat tccgcgatcc tgccagtcga
     1621 accgtttacc tgatacggcc ccataaacca gttctgcctg cggctcaatc caggagtctt
     1681 ctgttacatg atagcggtag cctgcttcag cacccacata caaggaatga gaactgtagt
     1741 cttttgttcc gagtccagca aaggtcgccg agtactcatt atcatggtga acatacttac
     1801 taatcaggtc gatataggca ccagagtcaa atatagcgga agcatacaga cctgccccga
     1861 cggacttcgt cttgccactg aatgcattac tgctggcatg actgtcggta tacgtcatag
     1921 tcagaccagt gaaaaggtca agtccatcca gctcatgttt tttatccaca ccaatctgca
     1981 catgggtgta gttgtcactg tatccaccag aagctgaacc tgctccgctc atgatgcggg
     2041 cccatgcacc ggcctcgcca ttggtgtcac gcagatcccc cattcgttta ttcaggttgt
     2101 tgacttctgt caggaaggct ttgtaattac cggacatata cgattttgct gcaccggaag
     2161 atgctgtatt ttcagccacc ttatatccgg tcagggtcca cgtggtatgt tcctgatcct
     2221 ctttcgtgct gatcacagga gatatgttgc tgaaacctat ggcctgagta ctggctttaa
     2281 atatctcttt attgcttcct tttggggctg ttatcaggtc aacgtttaat cctgagttat
     2341 tatttcccac tttctgtata tcgaccgaca gactgttatt gcttccggtt aactgatttt
     2401 taacaatgag tttatccgat tgatttacag actggctcag atcagcccgc ataatcacag
     2461 cagtattagt ggcatccagt gtgtttactg tcacagtatg gaaacccgaa ttctctatgt
     2521 tactaaacaa caaaagactg tcgcgggttt ttaatgattg cagggctgac tgtccgttta
     2581 ctctccataa ggtgttatac atgctggctg aggctgcagg agcatcaata cctccggtat
     2641 aagaagtatc aaagccaccc agcaaacctg tagccagcgg tgtgtgtgtg gttttgtcca
     2701 ggttatcttc tttatactgg tcaacaccaa acagaataga tgcttttttg tctgatttta
     2761 tattaccatt gacccaggca taattttgag cctttaacgt ggcaccatct tctgtcatat
     2821 tatatccatc tgacatatag attgccgggc tgtatttatt attttctgta ggggtgcctg
     2881 ttaatgtcag ggttccggcc agttcaatag ctttatctac atacattttt ccctgggcgg
     2941 taacagtagc tcctttgttg ataatcaggt ctgtattaat taaggataat gcaccagtca
     3001 tagtaacagt tgatttatca ccaacagtta ttttgctgtt ttgagcactc agactgccgt
     3061 tgaaactact ttcgcctgct gaatccccgg agcgaagctg ttgtctgaat ccaaaacctt
     3121 cgccagtaat gtttcttcct gagaacatgt caatgtatgc agttttattt cccagaacaa
     3181 tttgagagtt tttagcctct atgtgtcctt ttacattagc atcccggcct atactcaggg
     3241 ttgcgttttc taaatttaat ttccggaaat taaatttacg actttcccag tcgggctggg
     3301 aaaaagaggc tatctgattg ttggacttat attccgtatt gtaacgttta tttacagtgc
     3361 tttcctggtc ctttatggca gcagaatagt ctttttgaca taaaaaagga attggacagt
     3421 tgttattgcc ttctttaaat atggcatgtt ctgtcgcatg tccttgtaat gttaactgtg
     3481 cattccggac agagatatcg tttttgatat cgactgagcc atcaaagatc agattggcat
     3541 tattgttatg ttgctgggta gtattgataa tatgatttat gcttatatta cctgatacat
     3601 tcccatggta catatatgca tcagtattag taagtgataa tgttgattgc ttcacgtttg
     3661 agttcgttac ggttgttccc gcatctgttg ctgcgatttt ctgaaatgac tggtcatagc
     3721 cgtttagatc cagtgttccg ccatttctgg taaaatagat ttcgccatta ccgctttcgc
     3781 ttaaagcgtc tttggcattt atttttaccg tacctttacc tccggccatg taaattttat
     3841 tgaaggcttt ttcagcacta aggatgacag tcccattacc tattttaagg ttatttccct
     3901 gtgctatttt tacatccaga gtaccactac ctattttatg cagattatct ccggcaacgc
     3961 ccttaacatt ccattcaacg gtggtattat tatcaatatc tatcccggca cctttataag
     4021 acttatcttt tccgtacact ttatatgtct ggttcttatc gaatataagc ccgccaatgc
     4081 caagatcggt atcctgcttg agcgttaata cccctcctcc tgagacagac aggtccttat
     4141 cttttataat cgatgataac tcttgttttt taccatccag ctgcatagtg tttgtgaaat
     4201 tcttttttaa attgtttacc agaatattat taaataacga gtagtttgtc caggctatat
     4261 tggtactact gataaaacca actgtactgg taacaccatg gaaaacccat ttttgttgtt
     4321 ctttatcata tagataagaa ccagaaccac tatcgccaaa aattggtgag tttttaaaag
     4381 gggttctgtt ggtataaatg taactcttgt ttttccagtc cagattaaaa agacttgcgc
     4441 ttaacaagtc gggattatag ttttgtccaa acagatattg tttgccgttt aatatcgttg
     4501 aggttgttcc ggcacctgct ctgaagccaa ttatttgttc cttaccgttg tagtttacgc
     4561 catatctttt catcgcatct tcgggagaaa gtgagaagtc ggcactctcc gtaacccctg
     4621 ttgtttccac gacgaattta ttcagacgat gaaccgaaaa gtcgccagat gatactctgt
     4681 tacttgcttt atatgctgtc tgatcccatg actgtgttgt tattgcatga tgctgtgtac
     4741 cgttatgagt cgcagtaaca ctgtacgcac ctccaattga tgttgttgca cctttgttgg
     4801 aaacagcaga aaaatcaggt acaggtagtt ctggaaaatg aaatatcttt ccatctttca
     4861 tcattaattg aacatttgtt gcacccggtc tgaactctcc tctgttttga gcaaggtcaa
     4921 gataatcgcg ggtccatata ttatttatat ccagcattgc tgcaaatgct gtttcgccat
     4981 aacttccata tattgaggat aaaataatac cgaggataac tttctttctt gatgtaccga
     5041 cactaacttt ccgggtcagt tcagaaacag caactaatcc acctgtaata tgactatatt
     5101 tcagtgaata aattttattc atacgtgaac tccgatattt ctgagttgtg gttatgggat
     5161 agcatttaat tacaacgggg aactatcagt catggtacgt aactgataag ggtataatga
     5221 taatcactgt cagttatatt tgtatgacag atgtaacaaa atgaaaaaaa agataagcac
     5281 aagtgattga ttggttagat attgatcact tggtgtttgt attttgaatt ttgcgatcaa
     5341 aatttgatct ttgttgtttc cggtcaatcc tttacaggtt ttctgtatta tccgggggag
     5401 ttaagtctgt cgatgtccga cagacttttg atgatggaaa caacatgcag cgataatgtg
     5461 catgctgttt tgcaggtgga actcagaagg tgaactggaa ctgtgcgacc gcaccgtaag
     5521 tgttgtccgt gccgatcagg cctgcaacat caaggtgaac cacatcaaac ggtgagatcc
     5581 ccaccccggc cgtgaatgca ctgccgtcgt taccggccag attctgacgg taaccggcgc
     5641 gcagctgtgc ccatttccag gcattaaatt ctgtgccaat ggcggcaaac tgacggttgc
     5701 tgtcggaggt gaaaccgctg gccggggtca gatccacatc aaatgcagtg gtgaacatcg
     5761 cgttgtgcca ggagacaccg gccgtcacct gcgggcggat cctgaaggtt tccgtgatac
     5821 cgttcacttc ttttgtctca atactacggg agatcaggtt ctgtgcgacc agtcccagcg
     5881 tccagttgtc atccaggtcc gtactggcac cgatgtcggc gttgatgcca tttttcgtgt
     5941 tgtggtagcg gtcgcctttg aaggcgctgc tgtcataatt tctgaccagc acgttgtagt
     6001 tgaacaggtc gacgcgctgg aatttggggg ggaagcccag agaccatttc tgcccggccg
     6061 tttccagttc cgtagccaga gcaataccca catcagtgat cagtgccgtg cgggcacagg
     6121 cctgtgatgt caggcggctt ttgtccacct cctgcccggt gctgtttgcc acctcttcca
     6181 ggtaagtgag gtcagcatca gagactttac cgtcgacact gacggtgccg tgtgatttaa
     6241 ccatcagtgc ggcagagagg cgatcaccgg gaagggccgc cacagcagaa acgcccagct
     6301 gtgcggcggc atgtgtgtta cggaagtccc ggaggtgttc tttcaggcgt gcggcagcct
     6361 caggaacacc gtggttactt tcgatggcgc ggtcgaaggc cttccactca tcactgatcc
     6421 ggtcggcgtt gtcggtgata ttatccggat tggagagctg cgcgccgacg gacggaagca
     6481 ggatgctggg ctcatgcgcg acgcaaaatc cacgatgtat atatcagtac taaaagcgcg
     6541 acggcggaag aagctctgaa actaatcggt gaactgtacg ccatagagca cgatatacgc
     6601 gggttaccgg tgtctgaacg cctggcggtc aggcaaatgc agagtagacc gctcctgact
     6661 tccctgtata agctgatgca ggagaaagaa cacacgttat cgaagaaatg tcgtctggga
     6721 gatgcgttcc ggtatatcag gaagcactgg gctgcgttgt gcaacttctg tgatgatggc
     6781 ctggctgagg tagataataa tgcagcagaa agagcgcttc gtgcagtctg tctcgggaag
     6841 aaaacttttt cgaggatcac tcattccgtg tctgactgct tcagggttta attttgcttc
     6901 tgctgactga atgatgattg ggcagcaagt gcaatatccc acataaatcc agtcaactca
     6961 cgagccaccg ccgtaatggc tacttgtgat tttttccctt ttgctgtcag agttcggtaa
     7021 cgagaacaca atcgttgttg agctttccat gcaatatctt tggaatattg tgttacagag
     7081 tccggtcggt aatatataag ccatgctcca acttttgcag gtgtacgata agaccaggcg
     7141 gcttcgtaaa gcagacgtct cagttcgctg tttccaactt ttgttattcc tctgggacga
     7201 atactgtttc cactggaata ttcacctggc accagt
//
LOCUS       DAJVPT010000158         7180 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVPT010000158 DAJVPT010000000
VERSION     DAJVPT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..175
                     /locus_tag="OQM47_003637"
     CDS             <1..175
                     /locus_tag="OQM47_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1481639.1"
                     /translation="GMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQ
                     QARDGGRLSGAYR"
     gene            175..504
                     /locus_tag="OQM47_003638"
     CDS             175..504
                     /locus_tag="OQM47_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481640.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            504..1202
                     /locus_tag="OQM47_003639"
     CDS             504..1202
                     /locus_tag="OQM47_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1481641.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1207..1950
                     /locus_tag="OQM47_003640"
     CDS             1207..1950
                     /locus_tag="OQM47_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481642.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            1887..2489
                     /locus_tag="OQM47_003641"
     CDS             1887..2489
                     /locus_tag="OQM47_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1481643.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2550..6029
                     /locus_tag="OQM47_003642"
     CDS             2550..6029
                     /locus_tag="OQM47_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1481644.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            6097..6696
                     /locus_tag="OQM47_003643"
     CDS             6097..6696
                     /locus_tag="OQM47_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1481645.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6718..>7180
                     /locus_tag="OQM47_003644"
     CDS             6718..>7180
                     /locus_tag="OQM47_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1481646.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDD"
BASE COUNT         1623 a         1728 c         2302 g         1527 t
ORIGIN      
        1 aggcatggtg atgtttgccc cgcagtacaa cattgagatc aataacgacg gcacgaacgg
       61 gcagataggt ccggctgccc tgaaggtggt ttatgacctc gggaaaaaag cggccgcgga
      121 ctttatgcaa cagcaggccc gtgatggtgg ccggttaagt ggagcatacc ggtaatggag
      181 acgtttcact ggaaagtgcg cccggatatg aatgtggtat cagagccgaa agtggtgaca
      241 gtgaagctgg gcgatggtta tgaacagcgt cgtgcggcgg gactgaataa ccagttgtcg
      301 acttacagcg tgacgatacg tgttcgtaaa ggtgaacacc catctttaaa agcctttctg
      361 gaacggcacg gtggcgtccg cgcatttcag tggacgccac cttatgactg gaaactgatc
      421 agggtggttt gtcgtaaatg gtcggcaagc gtgggggcgt tatgggtgac tgtaacggcc
      481 gattttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg aatgaatgca
      541 cccgtgcgga gcagtcagcc cgggtggagc tctgggaaat cgatctgaca gaggtcggtg
      601 gtgagcgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc acctggcagg
      661 ggcggcagta tcaggcatac cccattcagg ggtcggggtt tgaactgaac ggcaggggct
      721 gtgctgcccg tccgacactg acggtctcta acctgcacgg catggtcacc gggatggcgg
      781 aagatctgca gagtctggtc ggcggaacgg tggtccggcg taaggtttac gcccgttttc
      841 tggatgcggt gaacttcgtc aacggaaaca gcgacgccga tccggaacag gaggtgatca
      901 gccgctggcg catcgagcag tgcagcgaac tgagcgcggt cagtgcctct tttgtgctgg
      961 ccacaccaac ggagacggat ggcgcggttt tcccggggcg tatcatgctg gcgaatacct
     1021 gtatgtggac ttaccgttct gatgagtgtg gttacacggg cagggcagtg gctgacgagt
     1081 tcgacaaacc aacaacggat atccggaagg acaaatgcag caagtgtatg cgcgggtgtg
     1141 agttgcgcaa caataccggt aatttcggcg gtttcctttc catcaataaa ctttctcagt
     1201 aaatccatga cacagacaga atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg
     1261 gagtcgtgtg gcttcgtggt gagaacgccg gagggagaca ggtatcttcc cagcgagaat
     1321 atctccggtg agccggagga acggttccgg atggctccgg aggactggct gcgggcacaa
     1381 atgcagggtg agattgtggc actggtccac agtcatcccg gtggtctgcc ctggctgagt
     1441 gaggctgacc ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg
     1501 acgattcaca agttccgctg tgtgccgcat ctcaccgggc ggcgctttga gcacggggtg
     1561 acggactgtt acacgctgtt ccgggatgct tatcatctgg cggggattga gatgccggat
     1621 tttcatcgcg gggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag
     1681 gccacagggc tgtatcaggt gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc
     1741 tgttttggtt catcggtgcc gaatcatgcc gccatttact gcggcgatgg tgagctgctg
     1801 caccatattc ctgaacaact gagtaaacga gagaggtata ccgacaaatg gcagcgacgc
     1861 acacactccc tctggcgtca ccgggaatgg cacgcatctg cctttacggg gatttgcaac
     1921 gatttggccg ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgcgcactgg
     1981 ccacacagct cccggcgttt cgtcagaaac tgagtgacgg ctggtatcag gtgcgcattg
     2041 ccgggcgtga tgcaggtgaa accgaattgt ctgcccgtct taatgagccg ctggcaaatg
     2101 gtgcagtgat ccacatagta ccgcgtctgg tgggagctaa aagtggcggt gtgtttcagg
     2161 cggtgctggg ggcagctgtt atggcggttg ctatatggat gccgggggta ggaattatgg
     2221 cgagtaatct gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc
     2281 tggcaccgaa agccagaact ccccgtacac agacaacgga taacggcaaa cagaacacct
     2341 gtttctcctc actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg
     2401 aaatgcgcgt ggggtcgcgg gtggtatctc aggagatcag cacggcagac gaaggggatg
     2461 gtggtcaggt tgtggtgatt ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg
     2521 gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc
     2581 cgcgcgaagc gaaggacaac ctgaaatcca cgcagttgct gagtgtgatt gatgccatca
     2641 gcgaagggcc ggttgacggt ccggtggatg gattaaaaag cgtgctgctg aacggtacgc
     2701 cggtcctgga tagcgagggg aagacaaact tttccggtgt tacggtggtg ttccgcgccg
     2761 gcgagcagga gcagacaccg ccggaggggt ttgaatcttc cggctcagag actgtgctgg
     2821 gtacggaagt gaaatacgac acgccgatca cccggaccat cacgtcggca aacattgatc
     2881 gtctgcgcct gaccttcggt gtgcaggcac tggtggaaac cacctcaaag ggggaccgga
     2941 atccgtcgga agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa
     3001 aagacatcac cattaagggc aaaaccactt cgcagtatct ggcctcggtg gtggtgggta
     3061 acctgccgcc gcgcccgttt aatatccgga tgcgcaggat gacaccggac agcaccacag
     3121 accagctgca gaacaaaacg ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt
     3181 gctacccgaa cacggcgctg gtcggcgtgc aggtggattc agagcagttc ggtaaccagc
     3241 aggtgagtcg caattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc
     3301 cgcagacgcg gcaatacagc ggtatctggg acggaacgct taagccagca tacagcaaca
     3361 acatggcctg gtgtctgtgg gacatgctca ctcatccgcg ctacggcatg gggaaacgtc
     3421 ttggtgcggc agatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt
     3481 cagtgccgga cggatttggc ggcacggagc cgcgcatcac ctgtaacgct tacctgacca
     3541 cacagcgtaa ggcgtgggat gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg
     3601 tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataaa gtgtggacct
     3661 ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg
     3721 ccctgaagga ccgccataat gtcgttgagg tgaactggat tgatccggac aacggtcatg
     3781 agacggcgac agagcttgtg gaggacacgc aggccattgt ccgttacggt cgtaacgtca
     3841 cgaagatgga tgcctttggc tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc
     3901 tgattaaaac ggaactgctg gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc
     3961 ttcgccatgt accgggtgat gtcattgaaa tctgcgatga tgattatgcc ggtatcagca
     4021 ccggtggtcg cgtactggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa
     4081 tcacgctgcc gtcctccggt accacgctga taagcctggt tgacggaaat ggcaatccgg
     4141 tcagcgtgga ggttcagtcc gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg
     4201 acggcgttgc agaatacagc gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt
     4261 tccgctgcgt gagtatccgt gagaacgacg acggcacgta tgccatcacc gccgtgcagc
     4321 atgtgccgga aaaagaggcc atcgtggata acggggcaca ctttgacggt gaccggcgcg
     4381 gcacggtgaa tggtgtcacg ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag
     4441 acagcgggga atatcaggtg ctggcgcgat gggacacgcc gaaggtggtg aagggtgtga
     4501 gcttcctgct tcgcctgacc gtggcagcgg acgacggcag tgagcggctg gtcagcacag
     4561 ccaggacgac ggaaaccaca taccgcttca ggcagctggc gctggggaat tacagtctga
     4621 cagtccgggc ggtaaatgcc cgggggcagc agggcgatcc ggcgtcggta tcgttccgga
     4681 ttgcggcacc ggcagcgcct gtcactattg aactgatacc ggggtatttt cagataacgg
     4741 tggtcccgaa actggctgta tatgacccga cggtgcagtt tgagttctgg ttttcggaaa
     4801 agcggattgc ggatatcagg caggttgaaa ccagcgcccg ctatcttggc acggcgctgt
     4861 actggatagc cgccagtatc aatatcaggc cgggccatga ttattatttt tacgttcgca
     4921 gtgtgaacac cgttggcaaa tcggcatttg tggaggccgt cggtcgggcg agcgatgatg
     4981 cggaaggtta tctgagtttt tataaagggt tgatcaataa aacgcatctc ggcaaggagt
     5041 tgtggacgca gattgataac ggtcagcttg cgccggacct gactgaaatc aggacgtcca
     5101 ttacgaatgt cagcaatgaa atcacgcaaa ccgtcaataa aaaactggaa aatcagagtg
     5161 ctgcaatcca gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt
     5221 gggctgtgaa gctgcaacag atgaaggacg gacgccttta tattgcgggt atcggtgccg
     5281 gtattgagaa tacgccagca ggaatgcaga gtcaggtgct gctggcggca gacaggattg
     5341 cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga
     5401 tattcatgaa cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc agcggcggta
     5461 atcctccggc attttccctg acacctggcg gacggctgac ggcgaaaaat gccgatatca
     5521 gcggtaacgt gaacgcgaac tccgggacgc tcaacaacgt cacgattaat aagaactgtc
     5581 gggttctggg aaaattgtcc gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca
     5641 aacctttccc acgggactcc cgggcaccgg agaggtggcc atcagggacc attaccgtca
     5701 gggtttatga cgatcagccg tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg
     5761 gtgctaaaca tgagcggaag aataacaata tttattcgtc atgccgcctg atagtgaaga
     5821 aaaacggtgc tgaaatttat aaccgaacga ccctggataa tacgctgata tatacgggtg
     5881 ttattgatat gcctgccggt cacggtcaca tgacgctgga gttttctgta tcggcatggc
     5941 tggtaaatgg ctggtatccc acagcaagta tcagcgattt gctggttgtt gtgatgaaga
     6001 aatccacagc aggtatcagt atcagctgaa ttttataacc cagatacggg caccagaaat
     6061 ggtgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgctgccatt
     6121 ttgtccgcag ccatctgtct ggctgtatcc ggtgcgcctg catgggcgtc tgaacatcgg
     6181 tccacgctga gcgcggggta tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg
     6241 aacgggatta acgtgaaata ccgttatgag tttacggaca cgctggggct ggtgacgtca
     6301 ttcagctatg ccaacgctga agatgagcaa aaaacgcatt acagtgatac ccgctggcat
     6361 gaggattccg tgcgtaaccg ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat
     6421 gaatggttca gcgcgtatgc gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc
     6481 ggggattatc tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccgggagt
     6541 gatgacaacc gccacagcaa cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg
     6601 accgaatccg tggccattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
     6661 gacggtttca tcgtgggtgt cggttataag ttctgattag ccaggtaaca cagtgttatg
     6721 acagcccgct ggttcaggcg ggctttttgt ggggtgaata tggcagtaaa gatttcaggt
     6781 gtactgaaag atggggcggg taaacctgtc gtaaattgtg cgattgaact gcgggccaga
     6841 agaaccagtc cgaccgttgt ggcacacgtt gttgccactt gcgtgacgga caataacggt
     6901 gcttatgtga ttgaggctga gccggggtat tacgaggttg cgcttcactg taacggctgg
     6961 cagccaaccc gtgtcgggga tattgatgtg gcaccgactg atgcaccggg gacactgaac
     7021 gcgtttctga atgcaccaaa ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag
     7081 gaaatggtgg cgcaggcgca gcagagtgcc ggggctgcag ccggaaacgc acagcagacg
     7141 gcgcaggatg tggcggcagc cgcaacggcc cgtgatgatg
//
LOCUS       DAJVPT010000159         6790 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVPT010000159 DAJVPT010000000
VERSION     DAJVPT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQM47_003645"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQM47_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1481647.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OQM47_003646"
     CDS             2089..2238
                     /locus_tag="OQM47_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481648.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OQM47_003647"
     CDS             complement(3005..3445)
                     /locus_tag="OQM47_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1481649.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OQM47_003648"
     CDS             3663..4052
                     /locus_tag="OQM47_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481650.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OQM47_003649"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OQM47_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OQM47_003650"
     CDS             complement(4607..5443)
                     /locus_tag="OQM47_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1481651.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="OQM47_003651"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="OQM47_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /locus_tag="OQM47_003652"
     CDS             complement(5793..6266)
                     /locus_tag="OQM47_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1481652.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="OQM47_003653"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="OQM47_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1757 c         1771 g         1677 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaatgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt cttatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAJVPT010000160         6768 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVPT010000160 DAJVPT010000000
VERSION     DAJVPT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..731
                     /gene="espL1"
                     /locus_tag="OQM47_003654"
                     /pseudo
     CDS             1..731
                     /gene="espL1"
                     /locus_tag="OQM47_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            903..1010
                     /locus_tag="OQM47_003655"
     CDS             903..1010
                     /locus_tag="OQM47_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481653.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1063..1821)
                     /locus_tag="OQM47_003656"
     CDS             complement(1063..1821)
                     /locus_tag="OQM47_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1481654.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2108..3037
                     /gene="pfkB"
                     /locus_tag="OQM47_003657"
     CDS             2108..3037
                     /gene="pfkB"
                     /locus_tag="OQM47_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1481655.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3138..3428
                     /gene="ghoS"
                     /locus_tag="OQM47_003658"
     CDS             3138..3428
                     /gene="ghoS"
                     /locus_tag="OQM47_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1481656.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3534..4394
                     /locus_tag="OQM47_003659"
     CDS             3534..4394
                     /locus_tag="OQM47_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1481657.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4435..4971)
                     /locus_tag="OQM47_003660"
     CDS             complement(4435..4971)
                     /locus_tag="OQM47_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1481658.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5118..5786
                     /gene="hxpB"
                     /locus_tag="OQM47_003661"
     CDS             5118..5786
                     /gene="hxpB"
                     /locus_tag="OQM47_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1481659.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5949..6539
                     /locus_tag="OQM47_003662"
     CDS             5949..6539
                     /locus_tag="OQM47_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1481660.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6672..>6768
                     /locus_tag="OQM47_003663"
     CDS             6672..>6768
                     /locus_tag="OQM47_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symporter"
                     /protein_id="HCS1481661.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKK"
BASE COUNT         1851 a         1606 c         1580 g         1731 t
ORIGIN      
        1 aataataact gcgcagaata gcgttggtac acctgcttta tttttggcta tgatgaacgg
       61 acatactgac aacgtgaaaa tatttatgca agaaattcag tcactggtag ataatcacat
      121 cattcatgaa gataatctgg ttaaattact gcaaactaaa agtgctaacg aaacacctag
      181 actttatatc tccatgttgt atggattcga tgaaataatc gatatctttc tgaatacatt
      241 aaccactcca atagcactaa gagcttttaa acaaaaaact ggtgatgagt attttagcca
      301 tgaaaataca tgatggtgag ccaggattat acgccgcaat ggaaaataat caccctttgt
      361 gtgtcacacg gttcctctct aaaattaatg gcatcgcctt taaatacaag ttgagcaaag
      421 ctaacatcat ggatttatta aaaggagcta cagcacaggg aacccctgca ttatacatcg
      481 ccatgagcaa gggtaatgaa gacgtcgtgt tatcttatat atcgacgctg ggtgcttttg
      541 caaaaaaaca ttcttttagt caacatcagt tatttacact attggccgct aaaaatcatg
      601 acaacatgtc agctgttcat atagccattc atcataagca ttataaaact gtagaaacat
      661 attatgctgc tataaatgtc atcagccaaa gcctgagttt tagtgctgat gaaataaaga
      721 cgtatttata accagctatt tacgatatat atcgatacat aattttattt ttttcataaa
      781 acgcagaaac agtgaccatg tgcacaacgc cacccgacgg ctgtaataac aaaaaatccc
      841 ttgatgcctg tcccttttgt tacactccgt tatcacgcac aagagatatg caggacactg
      901 gtatgccgac taaacgcttt gataaaaaac actggaagat ggtggtggtg ctactggcaa
      961 tctgtggcgc tatgttgttg ctacgttggg cagcaatgat ttggggctga gcagtgacaa
     1021 acgaagacag ccagaccgca taacacggtc cggcagataa ttttacatgc tgtagcccag
     1081 cgataaggtt gtccgacggt cggtatgttc cggtgcagac tctggaggtt cagagttcca
     1141 tgtgacgttg taagccacct tcagtccaaa atgttcatta atagcaacat ttaatgcgct
     1201 ttcggagttc agcgttgtgt cttccgcgcc aaagaccgaa acaccctgcg taaatttagc
     1261 gttgtcagtc aactgccatg cataagcccc ggaggcatag cccagcggct gggtttcact
     1321 ggcattgtcg gtatatttgt cgtaacgcac acctggaccg aattcaaagc ggaaactgtg
     1381 taccgggcca ttcaaaaact gacgaccata acccgcggtc aacacatcgc gctctcgata
     1441 gccgttataa cggtctgtca gccagcttgc ctgaccaaat aaatagtcat aatcagttaa
     1501 attaaagcgg ctacgcccgc ccgccgcata tttttctgaa gaacgctcat cattagaaga
     1561 agtattactg gcgttccccc acagcgacca ggcagtggtt tgtccatacc aggtcatggt
     1621 ggtgtcagcc gtaagtgagg agcttttcgt attgcctgat tgtgcaagat atcctgcgtt
     1681 cagattacct tcgaaaggtt ttttagcgct ggcaggatcg tccatgacag taaaaacgga
     1741 atcatcggca gctgcattca gtgacgcaaa catgcccccc gccaacataa cgatggcagg
     1801 aactgtcttc aaaagcttca tttatcaaga gtccgtacaa caaaaaaaga gaccatcgcg
     1861 gtcccggaaa ctttcttaag gatcaaagat tagcgtccct ggaaaggtaa cgaattataa
     1921 aaaggcgcga ataacttagc aatgtattct tatttcattt tttgaataag catgtggcga
     1981 aaacagattt ttatttatat atatttatct gcaaaatttt aaataaagct ctaataaatc
     2041 atattgttaa tttcttcact ttccgctgat tcggtgccag actgaaatca gcctatagga
     2101 ggaaatgatg gtacgtatct atacgttgac acttgcgccc tctctcgata gcgcaacaat
     2161 taccccgcaa atttatcccg aaggaaaact gcgctgtacc gcaccggtgt tcgaacccgg
     2221 gggcggcggc atcaacgtcg cccgcgccat tgcccatctt ggaggcagtg ccacagcgat
     2281 cttcccggcg ggtggcgcga ccggcgaaca cctggtttca ctgttggcgg atgaaaatgt
     2341 ccccgtcgct actgtagaag ccaaagactg gacccggcag aatttacacg tacatgtgga
     2401 agcaagcggt gaacagtatc gttttgttat gccaggcgcg gcattaaatg aagatgagtt
     2461 tcgccagctt gaagagcaag ttctggaaat tgaatccggg gccatcctgg tcataagcgg
     2521 aagcctgccg ccaggtgtga agctggaaaa attaacccaa ctgatttccg ctgcgcaaaa
     2581 ccaagggatc cgctgcatca tcgacagttc tggcgaagcg ttaagtgcag cactggcgat
     2641 tggtaacatc gagttggtta agcctaacca aaaagaactc agtgcgctgg tgaatcgcga
     2701 actcacccag ccagacgatg tccgcaaagc cgcgcaggaa atcgttaata gcggcaaggc
     2761 caaacgggtt gtcgtttccc ttggtccaca aggagcgctg ggtgtcgata gtgaaaactg
     2821 cattcaggtg gtgccaccac cggtgaaaag ccagagtacc gttggcgctg gtgacagcat
     2881 ggtcggcgcg atgacactga aactggcaga aaatgcctct cttgaagaga tggtccgttt
     2941 tggcgtagct gcggggagtg cagccacact caatcaggga acacgtctgt gctcccatga
     3001 cgatacgcaa aaaatttacg cttacctttc ccgctaacaa aaacattccc ccagcattgg
     3061 gggaatcacc accaacctgt cggcaacgcg tttctccgac tatgctcaaa agtcatgtga
     3121 taacaaaggg gtgaactatg gccagtggcg atcttgtcca ttatgtcata accgtcatgt
     3181 tgcatgagga tacattgact gaaattaacg agttgaataa ttacctgact cgcgacggtt
     3241 ttttgctcac catgacggat gatgagggaa atatccatga gctggggact aacacttttg
     3301 gacttatcag tacccaaagt gaagaagaaa ttagagaact ggtttcgggg cttacccaaa
     3361 gtgcaaccgg caaagatcct gaaatcacca tcacgacctg ggaggaatgg aatagcaaca
     3421 gaaaataaat ggtttttggg caataatcag tctgtggtgt gcgttagctc gtgtttttac
     3481 accgcattct tgcgctaacc ttatgatctg gcagacaaca tgggagagac atcatgtggc
     3541 aggcaatcag tcgtcttttg agcgagcagt taggtgaagg cgaaatcgaa ctgcgtaatg
     3601 aactgcctgg cggagaagtc catgccgcat ggcatttgcg ctatgcagga catgactttt
     3661 tcgtcaaatg tgatgaaagg gaactgcttc ccggttttac cgccgaagcc gaccaactgg
     3721 agttactgtc gcgtagtaaa accgtcaccg tgcctaaggt ttgggcagta ggcgctgacc
     3781 gtgactacag ttttctggtg atggattatc tcccacctcg tccgctggat gcgcatagcg
     3841 catttattct tggtcagcaa attgcgcgtt tacatcaatg gagtgaccaa ccacaatttg
     3901 gcctcgattt tgataactcg ctctccacaa ctccacagcc caacacctgg caacgtcgct
     3961 ggtcaacgtt ttttgctgaa caacggattg gctggcagtt ggaactggca gcagagaaag
     4021 ggatcgcttt cggcaatatc gacgccatcg tcgagcatat tcagcagcgt ctggcctcgc
     4081 atcaaccgca gccttctctg ttgcacggcg atttatggtc cggcaactgt gcgctgggtc
     4141 cggatggccc gtacattttc gaccctgcct gcgactgggg tgaccgagag tgcgacctag
     4201 cgatgttacc gctgcatact gaacagccgc cacaaatcta tgacggctat cagtcagtat
     4261 ccccgctacc tgccgatttc cttgaacgtc aaccggttta ccaactctac acgctgttaa
     4321 atcgtgcaag attatttggc ggtcagcatt tggttattgc tcagcagtca ttggatagat
     4381 tattagcagc atgatatggg ttgaggataa tggccgctcc gtgcggcctt ttgattaaat
     4441 aaacccaagc agagaaaaga acacataacc tgcagcaata caaataaccg gcaggatata
     4501 tagtgagaaa aactgcagga aaatagtatg gtgcggaaca acaatacggg actcaatttg
     4561 ttcacgcgtt agcccctctt cccctttggc tttttccaga atgagttgat cttcaacacc
     4621 ttcccgcaag aagcgcgcct ggcgactcat ccgggcaccg gaatcctgca acgccagtcc
     4681 gacaaaaatc aggatgaaaa tcacccagaa cataacgttc acaccaccat taaaattagg
     4741 ggtcggggag ttataccaga acagattcaa aaaaggcgta ttcgcctgca tcatatcgat
     4801 catgacatga gcaaagtcga gcatgaccgc attaatgcct tcctggtttt cctgccgggt
     4861 attcataaat ttcagcaaag aaatcaatgt agagatcaat gcaggtataa aaatcaccca
     4921 acccaaaatc cttttcaaaa cagcaatgcg tccagcttgt tgatacgtca tgagttctcc
     4981 ttgattaaga cgcgtcgttt cacttagttt acctgtagat atctgttttc gcccattctt
     5041 taaaggcgat atgataggcg cttaatcata agcacggctt aataccttac acataatgct
     5101 ctaaaggaga ggttgtaatg tcaaccccgc gtcagattct tgctgcaatt tttgatatgg
     5161 atggattact tatcgactca gaacctttat gggatcgagc cgaactggat gtgatggcaa
     5221 gcctgggggt agatatctcc cgtcgtaacg agctgccgga caccttaggt ttacgcatcg
     5281 atatggtggt cgatctttgg tacgcccggc aaccgtggaa tgggccaagc cgtcaggaag
     5341 tagtagaacg ggttattgcc cgtgccattt cactggttga agagacacgt ccattattac
     5401 caggcgtgcg cgaagctgtt gcgttatgca aagaacaagg tttattggtg ggactggcct
     5461 ccgcgtcacc actacatatg ctggaaaaag tgttgaccat gtttgactta cgcgacagtt
     5521 tcgatgccct cgcctcggct gaaaaactgc cttacagcaa gccgcatccg caagtatatc
     5581 tcgactgcgc agcaaaactg ggcgttgacc cactgacctg cgtggcgctg gaagactcgg
     5641 taaatggcat gatcgcttct aaagcagccc gcatgcgttc catcgtcgtt cctgcgccag
     5701 aagcgcaaaa tgatccacgt tttgtattag caaacgtcaa actctcatcg ctgacagaac
     5761 tcaccgcaaa agaccttctc ggttaatgac caggggcagt gatcacgctg cccctggttc
     5821 tttatctgaa tttgcattca actgacggat taatcgtcaa tttaagagaa agagttacac
     5881 cgtcaccact tccgtgcact gtataaaaat cctatactgt acgtatcgac agtttagtga
     5941 gttttatcat gacggcggaa ggtcaccttc tcttttctat tgcttgtgcg gtatttgcca
     6001 aaaatgccga gctgacgccc gtgctggcac agggtgactg gtggcatatt gtcccttccg
     6061 caatcctgac gtgtttgtta ccggacatcg atcacccaaa gtcgtttctt gggcagcgat
     6121 taaaatggat atcaaaaccg atcgcccgcg cttttgggca tcgtggtttt acccacagtc
     6181 tgctggcggt atttgcgctg ctggcaacct tttaccttaa ggttccggaa ggctggttca
     6241 ttccggctga tgcgctacaa ggaatggtgc tgggttattt gagccacata cttgccgata
     6301 tgctgacacc cgccggtgtt cccctgctct ggccatgccg ctggcgtttc cgcttgccta
     6361 tcctggttcc ccaaaagggc aaccaactgg aacgttttat ctgcatggca ttatttgtct
     6421 ggtcggtatg gatgccccat tcattacccg agaacagcgc tgttcgttgg tcatcgcaaa
     6481 tgatcaatac cttgcagatc cagtttcatc ggcttattaa gcatcaggtt gaatactaaa
     6541 aaggcaaaaa tcacctttct ggaataagca attccatttg aatataagag ccagctcaca
     6601 gttctgttaa tcttgcgcca acactatgac tgctacgcag tgatagaaat aataagatca
     6661 ggagaacggg gatgaacttt ccattaattg cgaacatcgt ggtgttcgtt gtactgctgt
     6721 ttgcgctggc tcagacccgc cataaacagt ggagtctggc gaaaaaag
//
LOCUS       DAJVPT010000161         6767 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVPT010000161 DAJVPT010000000
VERSION     DAJVPT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            58..1482
                     /gene="sbcB"
                     /locus_tag="OQM47_003664"
     CDS             58..1482
                     /gene="sbcB"
                     /locus_tag="OQM47_003664"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1481662.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1525..1752)
                     /locus_tag="OQM47_003665"
     CDS             complement(1525..1752)
                     /locus_tag="OQM47_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1481663.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OQM47_003666"
     CDS             complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OQM47_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1481664.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OQM47_003667"
     CDS             complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OQM47_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1481665.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OQM47_003668"
     CDS             complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OQM47_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1481666.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4574..5557)
                     /locus_tag="OQM47_003669"
     CDS             complement(4574..5557)
                     /locus_tag="OQM47_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1481667.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5603..6427)
                     /locus_tag="OQM47_003670"
     CDS             complement(5603..6427)
                     /locus_tag="OQM47_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1481668.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6510..6764)
                     /gene="yoeB"
                     /locus_tag="OQM47_003671"
     CDS             complement(6510..6764)
                     /gene="yoeB"
                     /locus_tag="OQM47_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1481669.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1826 a         1777 c         1586 g         1578 t
ORIGIN      
        1 ggttgctttt cgagttataa cagacagtta attaactaca gtggacaatg ctagacaatg
       61 acagacacag ataaacaacc taccttcctc tttcacgatt acgaaacctt tggcacgcac
      121 cccgcgttag atcgccctgc acagttcgca gccattcgca ccgatgacga attcaatgtc
      181 atcggcgaac ccgaagtctt ttactgcaag cccgcggatg actatttacc ccggcctgga
      241 gcagtattaa ttaccggtat taccccgcag gaggcacggg cgaaaggaga aaacgaagcc
      301 gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat tctgggctac
      361 aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat
      421 ccttacgcct ggagctggca gcatgataac tcgcgctggg atttactgga tgttatgcgt
      481 gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga cggtctaccg
      541 agctttcgcc ttgagcattt aaccaaagcg aatggtattg aacatagcaa cgcccacgat
      601 gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac gcgtcagcca
      661 cgcctgtttg attatctctt tacccatcgt aataaacaca aactgatggc gttgattgat
      721 gttccgcaga tgaaacccct ggctcacgtt tccggaatgt ttggggcatg gcgtggcaat
      781 accagctggg tggcaccgct ggcgtggcat cctgaaaatc gcaatgccgt aattatggcg
      841 gatttggcag gagacatttc gccattactg gagctggata tcgacacatt gcgcgagcgt
      901 ttatataccg caaaagccga tcttggcgat aacgccgccg ttccggttaa gctggtgcat
      961 atcaataaat gtccggtact ggcccaggcg aatacgctac gcccggaaga tgccgaccga
     1021 ctgggaatta atcgtcagca ttgcctcgat aacctaaaaa ttctgcgtga aaatccgcaa
     1081 gtgcgtgaaa aagtggtagc gatattcgcg gaagccgaac cgtttacacc ttcagataat
     1141 gtggatgcac agctttataa tggctttttc agtgacgccg atcgtgcagc aatgaaaatt
     1201 gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt tgataaacga
     1261 attgaaaagt tgttgttcaa ttatcgggca cgcaacttcc cggggacgct ggattatgcc
     1321 gagcagcaac gctggctgga gcaccgccac caggtcttca cgccagagtt tttgcagggt
     1381 tatgctgatg aattgcagat gctggtacaa caatatgccg atgacaaaga gaaagtggcg
     1441 ctgttaaaag cactttggca gtacgcggaa gagatcgtct aatttgaagc cgccgccgct
     1501 ggtacggcgg aggacaatgt tgagtcaggc tttttgaacg gtgatgctcc acgctgcatc
     1561 gccaatctgc tgataatcgg tgatggcatg tccttcttct gccgcccact gtgggattgc
     1621 ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg ccatttctgc
     1681 cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca catccagctt
     1741 tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt cgcgccttac
     1801 gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc ataaatacaa
     1861 ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta ccgatagagc
     1921 aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg ctacgtaatg
     1981 ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct gcaataaatg
     2041 cctccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc acgtcacccg
     2101 cgaccagaaa ttgaagaata ttggccgttg gggaagtgac tcctaaccca aacatgcgtc
     2161 cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt acagcagtga
     2221 caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccggttcgg cgcggcggta
     2281 aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag agcgttatca
     2341 ccagcaaaac ggtaaccaac ggccacacag acaaattaaa agtctcagca atagagttat
     2401 gttcagtagt gtagtgctgc aaggtttgat ttaaaccact ggcgtgtgga gaacgcatca
     2461 ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat ccctcacccg
     2521 cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc ccgaaaagat
     2581 acccacctat aacagtacca agccacggga acgccccggc ttcgtaagtc agtaggcccg
     2641 cctgaattaa cgcaaaaacc cccacgcttt gtgccgaaat tgcaatcagc aaggcgtaaa
     2701 acatgcgatt atttttcgcg atatacatat cgcgaaaacc accagtcagg caaaaacgcc
     2761 cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat atcattgaaa
     2821 acataaatta attaaccaga tgaatgttaa tggggaaatt tttcatgact ggtggaatgc
     2881 agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga tttcgataaa
     2941 aaagaataaa aaaaccggag ccaaatgttc attcgactcc ggtttattat tagaaaagat
     3001 gattatgcta cgtcttcgta ctgcggaacc ggattacgga agctcttggt gacgcaagcc
     3061 aggtaaatca gaccgatagc agcccagatc agacccagaa ccattgagct ttcttccagg
     3121 ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag atactggaag
     3181 tgatctttca gcgtcttgtt acgcttctca cggatccaga actgcgagat gaccgacagg
     3241 ttaacaaagg tgaacgccac cagcgcacca aagttaatca gcgccgttgc cattaccagg
     3301 tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat cgccggagta
     3361 cgccatttcg ggtgtacata accgaagaag cttttcggga acacgccgtc acgacccatt
     3421 acgtacatca gacgcgctac gcctgcgtgc gccgccatac cggacgccag tacggtaatg
     3481 gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag catgatttca
     3541 ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag ctgcaggaag
     3601 taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg
     3661 atcacgcgct ctgcatcttt ggtttcttcc gacagattgc tgatgccgtc aaagccggta
     3721 aaggagaagc acaggattgt cgccccggta atcatcggga taacatgcgc atcgccagac
     3781 cagaacggac gcgtgctcgc cagcgtacca gcgccttcac cttcaaatac gccataaaca
     3841 accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac agtgttgacg
     3901 ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac cagcgccacc
     3961 acaaacatcc acgatggaat ggaaggcacc agagcttcaa aatagatttt cgccagcaga
     4021 atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac cataaagccg
     4081 acagtcgggc taatggattt ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg
     4141 cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc gaacgcatag
     4201 gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaatgtatc aaacagcgtc
     4261 atcggctgca tataggctag acccatcatt acaaccggaa ctaacgtaag cgttttacgc
     4321 aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggttattct cctttacggt
     4381 gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag cgacaacaac
     4441 tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta tgaatgattg
     4501 gtttgaagca aaaaataacc gacgctaatt aaacgtcggt ttttaatcat tttttgacag
     4561 cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt gtcataataa
     4621 tttttctttg aatggctgat tttccgtcat cagattttgc ataaacaccg gcgatcgcac
     4681 tatttgctaa aatttcatct cgccaccagc cgctggcaag ccctgcggtc tgttcattcc
     4741 agccaatcca gacaggttca aagagagttt gggcgaccac tttcttctcg attaacgcgc
     4801 cactatcgag aaaacgttgc gccagatacc gtggtaaata gccgcagcca aggccgctaa
     4861 tttgcagctc cagtttggtt ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa
     4921 gttgtgaggc cgtagaagcg cctgcctgcg cagtatcccc caccacaatc gcccgatatc
     4981 gtttaatgat acgacggttt aacggttctt cttcctgggc taacggatga tgaggcgcga
     5041 ccgcaaagac ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg gaagatggtg
     5101 gctcatgcat cgcgccaacg ataatatccg ctcgcccctg agtcagggca tcccaggaac
     5161 caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga taaaaagctt
     5221 caatgagagg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt
     5281 tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa
     5341 cttcccgccc tttctctaat aacatttttc cggtgcgggt gaatttagcg cggtgtccgc
     5401 tacgatcaag caattgaata tttaggtcgc tttccagctt gtgaacggtg taactcagag
     5461 cggacggcgt cttgtagagt ttggctgacg ccgccgcaaa actgccctct ttttctatgg
     5521 catcgaggat catcaacaca tccagcagtg gtttcataca cgcccccttg cagtgcatgc
     5581 ggcgtcctgc tggcgtgatc gtttactcca acggcattac cagcggatcg ggatactggt
     5641 attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct ttgccgctgt
     5701 ccagactatt tctgaactgc ggaggttgca gccccagtaa acgggccatc tgcggataga
     5761 aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg cctttaggtg
     5821 cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa ttaacaccat
     5881 gttcaccatc aggggcggtt tttccggcaa agaagcgtcc cggatgacgt cccggtccga
     5941 ccaggcccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc agccagtctt
     6001 cgagttcttc taatactcgt ccactgttgg ttaccggatt acgcggggta gtttctttaa
     6061 ccgtgccttg cgcgtcgcca tagacagatg ttgagctggt aaaaataatt cggggaatac
     6121 gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac tcatcgccgg
     6181 ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccata agggcatcca
     6241 gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta tcaatgccac
     6301 tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact tgccagcctc
     6361 gcgctgaaag tgacatcgcc agcggcatac ctaaccaccc taacccgaca attgcgacct
     6421 ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag cgtatcaaaa
     6481 ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc tgcaatgagc
     6541 agtgaatcat cagtaaccgc gtataccaga cggtgctcct ctgtaatgcg tcgggaccag
     6601 aaacctgaca aattatgttt caggggttct ggtttcccct taccttcaaa tggtgttctg
     6661 cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt ttcctgccag
     6721 tacagataat catcccatga ttcctcagac cagattagtt tcactca
//
LOCUS       DAJVPT010000162         6727 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVPT010000162 DAJVPT010000000
VERSION     DAJVPT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(376..810)
                     /locus_tag="OQM47_003672"
     CDS             complement(376..810)
                     /locus_tag="OQM47_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1481670.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(798..1199)
                     /locus_tag="OQM47_003673"
     CDS             complement(798..1199)
                     /locus_tag="OQM47_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481671.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1459..2028)
                     /locus_tag="OQM47_003674"
     CDS             complement(1459..2028)
                     /locus_tag="OQM47_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1481672.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2773..2916)
                     /locus_tag="OQM47_003675"
                     /pseudo
     CDS             complement(2773..2916)
                     /locus_tag="OQM47_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3250..3516)
                     /locus_tag="OQM47_003676"
     CDS             complement(3250..3516)
                     /locus_tag="OQM47_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481673.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3657..3863
                     /locus_tag="OQM47_003677"
     CDS             3657..3863
                     /locus_tag="OQM47_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1481674.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3958..4560
                     /locus_tag="OQM47_003678"
     CDS             3958..4560
                     /locus_tag="OQM47_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481675.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNINYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5760..6677
                     /locus_tag="OQM47_003679"
     CDS             5760..6677
                     /locus_tag="OQM47_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481676.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         1663 a         1754 c         1585 g         1725 t
ORIGIN      
        1 gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt tggtggacac
       61 cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct taccactatt
      121 ttatccctgt tgaatttggt taagccggtg cagacgcgcc gggaaacgca cacctgaacc
      181 ggcacaataa aatacaatac aaccccctgt tactggagat acgaatggaa tgacgaggca
      241 gcctgtcagt cacagggggc tgttggcata aaatcaacgg aaaggccacc aacaccggaa
      301 ttaagctcca cagtccccgc actggcaatc cattcccggg aaaggcatca ccgcaaactg
      361 ctccggaggc aaccattaca gcctctgtaa cggattatca gccctggcat cttccgacgg
      421 cacctcctgc cgcccccacg ccctgctgta taccccggta ccgcagcgtg ggcagcactt
      481 ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct tcaccgtata
      541 atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg ccgggtcaag
      601 ccaggtacat cgcagcagga ttttttcagc gacagtcatc tggtcatgtt caaaccacgg
      661 tggttgtccg tcattgcccc gctgaagtgt taacgggcat ccacaggaca cacagcacaa
      721 aacgtcctgt cggttgtgct tcgcagcttc tttcgccgta acaaaatggc ctttgctgtc
      781 attcgccata taacagttca gaattcgcat atttcctcct gagttgtccg gggaatactg
      841 aatcctccgg tctggcagtg tgtgcagtac tgctccccat aataatcgtg gtgacattgt
      901 ctgcagtgcc agctggcttt acgcaccacg ggtaaggcat ccggtacgaa ttgctgcaga
      961 cgtttaatca actgtatttc tctgcgctcc ggcctgacat aagggcactg ttgaccgtgc
     1021 tctgtcagcc tgtcgtcagt gtgttcaaac cagggaagtt cagtttcgta ttgcggatgg
     1081 tatctgagtg cactgccaca caagtggcag gtgtagttag cataaggtgc ggcctgtgca
     1141 gtacgggcgc ccgtcagacg tccgttgcca tcaagagcga taaaggattt tgcgtacata
     1201 gtcatatacc tcagccgcca gacgacacgc aggcaacagc cgtcccggta cgggcagcgt
     1261 ggtcagggtg tgaatggcgg ttcagttaaa atctggactg gagtttgggt aaagcaggct
     1321 ggttgctgtt acagcactgt ggttgtcaca caatggaaca ctgacgtaaa aaacagtcag
     1381 tgggcgagtg gatacatcgt tgccgccaga caacaaccca tacttttggt ttgccggata
     1441 ttggcgcgcc agtcgtgctc aactccggct acagccaaaa ttgcgctcac cggtgcagtg
     1501 agctttggta tgctccttcg ccagatagtc agcacgttcc agcacttgct gaaagccggt
     1561 gtcatcatta cgcgccagcc acaccgccgg ccaggccgga aaatgcacca gcgtggcatg
     1621 gcacccgaca tccactccca gggcactgca ccacgcctgt ttaatcatcc cggccagaga
     1681 ctccggagcg cggtaatcac cggcacgaca ccaggtatcc cggttgacca gcagcatcag
     1741 gtgatagtgt tttttacctt tgcactcccc aaactcccgg gcccagacgt aatgcagggt
     1801 tgtgggatgc acgcgtttac cttcccgatg cttacgtttc tggtaagcgt cgattcgggc
     1861 tttcagggca ttgatgaagc gggatatcac agctgcgtcc gttgctgccg gtacatccgg
     1921 gagacgcagg tcaacccgca atgccgtcag gcggggatga acattcagtg cgtgccgcac
     1981 cgtctcacga atacgttgct gccagaaggg gttgtatttg taggtcatgg ttaaatctcc
     2041 gtatggttca tacggaatag ccacgtcgta aaaaatacgc agagtccctg aagtgacctc
     2101 cggcaggaca ctgtctcagg cactctgtga taacccgaac ggtcgtctcc ggactgacgg
     2161 ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg ctgtgaggag
     2221 ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat tactcataat
     2281 acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg gaatacagac
     2341 cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac tgtcagcgtc
     2401 agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg agctgacagt
     2461 gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta aattttccag
     2521 ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt ctgacaaata
     2581 cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat acctgaaaat
     2641 cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat tctttgttgt
     2701 cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt catcgtagcg
     2761 ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat cgtaaagttt
     2821 gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt ccagctccgc
     2881 aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg acgtattacg
     2941 ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc ggggatccgg
     3001 acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga gggatttctc
     3061 agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca gttcatgtct
     3121 tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg catcagcgtt
     3181 catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg agagatttac
     3241 tctcttagct tactctggca aatcctttac gttactctct gatgacttct tatatttata
     3301 ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc caggaacggg
     3361 ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc ctgcttctct
     3421 ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag catcttttcg
     3481 caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt ttatctcctg
     3541 gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg gtgaaggtgg
     3601 cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac attctcatgg
     3661 ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc actcaactga
     3721 ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt ccggctccca
     3781 tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg ctacaggcgc
     3841 gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca gcagacgtaa
     3901 tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa tatgaaaatg
     3961 aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa atggctgacc
     4021 ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcaa ctacttccat
     4081 aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc catcgtaccc
     4141 cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc cctggatgat
     4201 gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc gatgctggaa
     4261 ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa taaactgatc
     4321 agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg gctttgctgg
     4381 cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa acggaaatca
     4441 gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc actgacggat
     4501 ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag ccgcaactga
     4561 tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc cggcccccat
     4621 gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg tcttaagggc
     4681 atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg atgttcttta
     4741 cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt attcaccaga
     4801 aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg acagcgatcc
     4861 cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat ccgggaatgt
     4921 ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc atcataaata
     4981 atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg aaataatatc
     5041 cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta tcagaaatgt
     5101 tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga taatgtatat
     5161 cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc cttttcagac
     5221 ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt ttataactct
     5281 ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg tattaacaga
     5341 cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct gctgccctct
     5401 gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa gtgatgctgg
     5461 cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg tctgcagtct
     5521 ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat gataacaggt
     5581 attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc ctgtcagaat
     5641 gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt tataaacttc
     5701 tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg agaaagagga
     5761 tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg tattactctt
     5821 attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat cccccggaac
     5881 gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt tcagattttg
     5941 aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg gtccccctgg
     6001 aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg tatctgttga
     6061 tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa aagaataaac
     6121 tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca tcgacacagt
     6181 tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg gatgagtgga
     6241 taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct ttcagttacc
     6301 tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag ggaaaaaata
     6361 ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac gcaattcacc
     6421 tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata aaaaatttca
     6481 gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag gaagataaag
     6541 tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa ttatcgaggg
     6601 tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag tataaaaggt
     6661 accgacattc cagataattt cagacatata ttatctccgt gagtcccccg ccacctttcc
     6721 ggtgtgc
//
LOCUS       DAJVPT010000163         6666 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVPT010000163 DAJVPT010000000
VERSION     DAJVPT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1321
                     /locus_tag="OQM47_003680"
     CDS             <1..1321
                     /locus_tag="OQM47_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1481677.1"
                     /translation="FTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTET
                     FLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFD
                     YFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLA
                     YNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTI
                     ILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMD
                     FYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFW
                     DNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKD
                     NLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1373..2530
                     /locus_tag="OQM47_003681"
     CDS             1373..2530
                     /locus_tag="OQM47_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1481678.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2496..2618)
                     /locus_tag="OQM47_003682"
     CDS             complement(2496..2618)
                     /locus_tag="OQM47_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481679.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2821..4506
                     /locus_tag="OQM47_003683"
                     /pseudo
     CDS             2821..4506
                     /locus_tag="OQM47_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4544..>6666
                     /locus_tag="OQM47_003684"
     CDS             4544..>6666
                     /locus_tag="OQM47_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1481680.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNA"
BASE COUNT         1951 a         1457 c         1413 g         1845 t
ORIGIN      
        1 ttttactaac ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac
       61 cggtcgagct cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc
      121 gctaacagaa actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt
      181 aggtaaatgg cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg
      241 tgactatcat gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg
      301 ctttgattac tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact
      361 gttcaaaaat cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga
      421 ggcaattggc gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc
      481 ttataatgct ccgcacctac caaatgataa tcctgcaccg gatcaatatc agaagcaatt
      541 taataccggt agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg
      601 tgtaaaacgc attctcgaac aactgaagaa aaacggacag tatgacaata caattattct
      661 ctttacctcc gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa
      721 aggctataag agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa
      781 aggaaaactt caacccggta attatgacaa gctgatttcc gcaatggatt tctacccaac
      841 agctcttgat gcagccgata tcagcattcc aaaagacctt aagctggatg gtgttccctt
      901 gctgccctgg ttgcaagata agaaacaagg cgagccacat aaaaatctga cctggataac
      961 ctcttattct cactggtttg atgaggaaaa tattccattc tgggataatt accacaaatt
     1021 tgttcgccat cagtcagacg attacccgca taaccccaac actgaggact taagccaatt
     1081 ctcttatacg gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt
     1141 aggtctctac aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca
     1201 ggtcgttaaa gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct
     1261 tagcgaggta aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata
     1321 actaaacctt catgcggcgg atttttccgc cgccttattg agcgagatag cgatgcacgt
     1381 tacagccaag ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga
     1441 aaaagagtcg cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat
     1501 caaacaatat atcgaagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc
     1561 cactctggct ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg
     1621 ccaaaaacgt atttttaata cattacaaac gaatggcatt ttattgaata atgaatggtg
     1681 tgcctttctc aaagaacatg aatttctggt tggtatctcg atcgatggcc cccaggagtt
     1741 acatgaccgt tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc
     1801 catcgagcgt ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca ttaataacgt
     1861 taatgtccat tatcctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat
     1921 gcaatttatc gaattgctag aaaccgggac gccgaatatt gatttcagtg gtcatagtga
     1981 gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc
     2041 aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcattc gtcagtttga
     2101 aagctttgtc agccgttttt tggggaatgg gcatacaagt tgtattttcc aggagtcctg
     2161 caaggataat ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta
     2221 tccacagtac aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca
     2281 actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat
     2341 ctgcaatggc ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta
     2401 tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt
     2461 tgagttagct aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga
     2521 gaataattaa taacgctctc ttttaactcc attcttagcc tccttcttat gaccttaaca
     2581 aggcacccga cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat
     2641 cctggcttac gtggtatgcg tcagggcatt agcaataccc ttgattatca atagcataac
     2701 cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga tgcttaaata
     2761 aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca attttcgcta
     2821 atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg tctgctgaag
     2881 cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat tatcctcgca
     2941 atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa tgattttttc
     3001 aatgcgctaa gccagaaaga aaccgataag ctttggcagc tcgttctttg gttccctgcc
     3061 ctgctgggga tctttgtatt aatatccgca aacaaaacat ggttaattaa actactgact
     3121 attcgctggc gcgaatggtt aacagattac taccttaatc gctggtttgc agataaaaac
     3181 tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga ccaacgtatc
     3241 gctgaagaca ttctcttact gatcaacaaa acgctcagtc tttcatttgg cttcatccag
     3301 tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg tacgctctca
     3361 ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac cgtcgtgctc
     3421 attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg tccgcttaat
     3481 gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca taataagcag
     3541 gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt gcgcgacaat
     3601 tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac
     3661 tggcaaaata tttattcgcg ctcgcttagc gttcttccct actttctgtt attgccgcag
     3721 tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc atttatgcta
     3781 gtatcgaaca atttaagctg gtttatttat aaatatgacg aacttgctga actggctgcg
     3841 gttatcgatc gcttgtatga gttccatcaa ctcactgaat agcgccctac gaataagcct
     3901 aaaaattgcc aacatgcggt acaagtggct aatgcgagta ttcgtacgcc tgataataag
     3961 atcatattag agaacttgaa ctttcatgtt tcgccaggca aatggctatt actgaaaggc
     4021 tactctggtg cgggaaaaac cacactgctt aaaacattat cccactgctg gccgtggttt
     4081 aaaggtgata tttcttctcc tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa
     4141 agcggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg
     4201 ttgagcgaag tactgcatca ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac
     4261 gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct ggcacgatta
     4321 attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct tgaggaacaa
     4381 gaggctatac gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg
     4441 gttacacatc aacccggtgt ctggaacctg gctgatgata tttgtgacat tagcacggtt
     4501 ttataacaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc gagttcttat
     4561 tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta
     4621 catgtatttt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg taaacggcaa
     4681 cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca atatcagcga
     4741 tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct cgttgaacca
     4801 gggagatatt cgccctgaga aaatctctat tcacggtgcg tcgccctacc agaatgccta
     4861 tttgattgac ggtattagtg caactaataa cctgaaccca gcgaatgagt ccgatgccag
     4921 tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca gcttactgga
     4981 caatgtgacg ctttatgaca gttttgtgcc ggttgaattt ggtcgcttca atggcggggt
     5041 aattgatgca aagatcaaac gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg
     5101 cactacgcgt ttggactggt taacatcgca tatcgatgag aataacaaga gcgcatttaa
     5161 tcaaggttct tcaggaagta cctatttctc tccagatttt aaaaagaact tttatacctt
     5221 gtcgtttaat caggagctgg ctgataactt tggcgttacc gccggtttat cgcgccgcca
     5281 gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg gtcgggcaca
     5341 gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca gcgaccgctt
     5401 tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata ccagcacctt
     5461 cccgcagtct gatcgcgaaa tgggtaataa atcctatggt ctggcatggg atatggatac
     5521 gcaactcgca tgggccaaac tacgtaccac cgttggttgg gatcatatta gtgattatac
     5581 ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg atattacagg
     5641 gcgttgtacc cgtggcggat taggacacat ttcccaggct gtagataatt acaccttcaa
     5701 aacacgcctg gactggcaaa aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg
     5761 cgcggaatac atctattccg atgcatggac tgaacgccat aaccagtctg aatcctatgt
     5821 gattaatgct gcaggaaaga aaactaacca taccatttac cataaaggta aaggcagcct
     5881 gggaattgac aactacacgc tgtatatggc ggatcacatt agctggcgga atgtgtcgtt
     5941 aatgcccggt gtgcgttatg actatgacaa ctatctgtca aaccacaata tctccccgcg
     6001 ctttatgacg gaatgggata tttttgctga tcaaacctca atgattaccg ccggttataa
     6061 ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca atagctggac
     6121 ggaatcggta tcaggtaata aaaccctgac gcgttatcag aatttgaaaa cgccttataa
     6181 cgatgaactg gcaatgggat tgcagcaaaa aatcgataag aacgttattg cacgcgcaag
     6241 tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat
     6301 tactgaatat aacaacgatg gcaaaaccaa aacgcattcg tttaacctca gttttgaact
     6361 ggccgaaccc ctgcatatcc gccaggtaga tattaacccg caaattgtct ttagctatat
     6421 caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata ccggtgataa
     6481 ccaggtggtt tataacggta atctggtctc ttacgatagc gttccagtgg cagattttaa
     6541 taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg ggttagtgtg
     6601 ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatccttg gtaagacaaa
     6661 tgcgca
//
LOCUS       DAJVPT010000164         6663 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVPT010000164 DAJVPT010000000
VERSION     DAJVPT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1233..2180
                     /gene="icsP"
                     /locus_tag="OQM47_003685"
     CDS             1233..2180
                     /gene="icsP"
                     /locus_tag="OQM47_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1481681.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2742..3114)
                     /locus_tag="OQM47_003686"
                     /pseudo
     CDS             complement(2742..3114)
                     /locus_tag="OQM47_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3358..3477
                     /locus_tag="OQM47_003687"
                     /pseudo
     CDS             3358..3477
                     /locus_tag="OQM47_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3727..4593
                     /gene="ospB"
                     /locus_tag="OQM47_003688"
     CDS             3727..4593
                     /gene="ospB"
                     /locus_tag="OQM47_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1481682.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4922..5662
                     /gene="phoN2"
                     /locus_tag="OQM47_003689"
     CDS             4922..5662
                     /gene="phoN2"
                     /locus_tag="OQM47_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1481683.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6022..>6663)
                     /gene="ospC4"
                     /locus_tag="OQM47_003690"
     CDS             complement(6022..>6663)
                     /gene="ospC4"
                     /locus_tag="OQM47_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1481684.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2100 a         1238 c         1268 g         2057 t
ORIGIN      
        1 aacttaattg gggctccctc ttccttttat ggtgtaattg tttgattgaa tacttccggg
       61 gatttcagta tgaaatgaag tatatttaat atactttaca aaaagtttgt attagtggaa
      121 taaggacaat agcttatcca aactgccaag cctctttatt ataagtaaga tctggcactg
      181 gaatattctc aaaaggacta acatcatcac cacgaccttc aaccaaccat tttattgcat
      241 ttaaatccca gaatagttca gtagtggtca atttgggaaa cgcacaatat tgtacggtta
      301 cgttatcgca atagagtgaa ctctcgccct atacggcgtt ttcaaggatt agtccttaat
      361 cggacaacca cattaaagca taataaataa ttaagcttta ataatatact atagatacag
      421 ttctattata aaaaatacta ttttaggtga tgcattaaat tagtagaata tttattcttt
      481 tataaaagca ccaatgaatt gtccaagtaa acatgagata aaatgtgtag aggataactg
      541 agtttggtca ctttaactgt attagtcgca gtctgactct cgactttaaa aggatgggaa
      601 ttacctgtac tgatagtgag aaagctttcc attgaaataa gtcaaataaa atttgatggc
      661 ttcagtgcta aagtgaggtg cccgctagcc gtattcactc gaatcggaat tcaatgtatt
      721 gcttctgcca tttccataat atttcacggg aaaaacaaaa tctgaaaaac tatacttaag
      781 tgttgtattg agtcagttga gatacaaata catatttatc agcacacgtt acgtcaaata
      841 caggaggttg tcatgttggt actgaaaggc acgttggcta ttgcagccgt gatgacaatt
      901 tattgtctta tcgttgttct tatggaacgc ttttctgatt gatttcatat tggcgagaga
      961 acaagaaggt gagtagaatt gtcgcggaat cctgaaacta tcagcctcag gtatgaacac
     1021 caaaaggtag gtagagaaat ctatggcccc cgttacttta ttcgcacaat gaggtatttt
     1081 tttattttta caaaagttca ataaatatta taataaaaga tatgttcttg gtaacataaa
     1141 caaatattta attaaaataa tgcttggcat tgacgtttat cttataaagt aagaagatca
     1201 tggacatttc aaccaaaaaa gtagagttct cgatgaaatt aaaattcttt gtacttgcac
     1261 tttgtgtacc tgcgatcttt actacacatg ctaccactaa ctatccactt ttcataccgg
     1321 acaacatcag tacggatatt agtctaggat ctctgagtgg caaaacaaaa gaacgcgttt
     1381 atcatcccaa ggaagggggg agaaaaatta gtcaactgga ctggaaatac agtaatgcga
     1441 ctattgttag aggtggcatc gattggaagc taattccaaa agtgtctttc ggagtttccg
     1501 gttggactac tttaggtaac cagaaagcaa gcatggttga taaagactgg aacaattcca
     1561 atacccctca ggtatggacc gaccagagct ggcatcccaa tacgcatctc cgtgatgcta
     1621 acgaattcga gctgaatctt aaaggttggt tattaaataa tttggattat cgacttggac
     1681 taatagcagg ttaccaagag agtcgttaca gttttaatgc aatgggaggg agttatattt
     1741 atagtgaaaa cggggggagc aggaataaaa aaggggcaca tcctagtggt gaaagaacaa
     1801 taggttacaa acagctcttc aaaatacctt atattggatt aactgctaat taccgccatg
     1861 agaattttga gtttggagca gaactgaaat atagtggttg ggttctttca tctgatacag
     1921 ataaacacta tcagactgag acaattttta aagatgaaat aaaaaaccaa aattactgct
     1981 ctgttgctgc gaatattgga tactatgtca cccccagtgc aaaattttat atagaaggct
     2041 ccagaaatta catttctaat aaaaaaggtg atacatctct ttatgagcaa agtaccaata
     2101 tatctggcac cattaaaaat agtgcaagta ttgaatatat tggttttctc acttccgcag
     2161 gtataaagta tattttttga attggttcac cgagttgatt acgttttcat ggctaaaaat
     2221 tgccgcccat tgtgagtcat attcgccctg actttccaga accatacgaa ctgcccgttg
     2281 acggacttcg ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg
     2341 tttagtctcc aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta
     2401 ctgttggcat taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg
     2461 ttctcggggt atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg
     2521 aaagcgctga tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc
     2581 cagcactcta tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc
     2641 agaaattaag gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat
     2701 ttcttgatta tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg
     2761 ttatcattgg cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc
     2821 ggatgccatt cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg
     2881 tcaggtttgg gcatgatact gatgtagtca cactttattg ttttcacgaa gctctttgct
     2941 attctgttac tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat
     3001 ccgggtgaac tggcgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt
     3061 cactggagac gccggaagct cgttgccgaa gcggtgttcc accgctccca gcatgtcgcc
     3121 aacgggagag catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag
     3181 tacgctctga acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta
     3241 aaacagcact tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta
     3301 aagaggttgt gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat
     3361 catacttggc gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa
     3421 cattaccgga acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga
     3481 tgatctccac ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt
     3541 gtagttgaaa ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc
     3601 cagaatataa ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca
     3661 tactatatga aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat
     3721 gtaggtatga atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa
     3781 agcatatcag atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact
     3841 cacccaaaag cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt
     3901 ctatctatta tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat
     3961 gtttttaaaa atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca
     4021 cagagtgaac caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt
     4081 tctgtaaaaa aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag
     4141 gtttatattc ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt
     4201 attgatgtac aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat
     4261 atccgtttta cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc
     4321 cctgctgaaa gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct
     4381 ttgctagagc agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa
     4441 atatccggct atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat
     4501 tatcgttcaa cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa
     4561 aagactttta ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt
     4621 gtttgcattt aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa
     4681 aatcacaaat catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca
     4741 ttcttttacc acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga
     4801 ctattttttg tttttccatc actctgttca aatttttccg catgacttgt gttttttgta
     4861 atacagctcg ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat
     4921 tatgaaaacc aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc
     4981 agcaaatgct ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc
     5041 aatacttccg ccgcctccgg cagagaattc agtagtattt caggctgaca aagctcatta
     5101 tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata
     5161 ctacgagaat tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa
     5221 taccccaatc ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt
     5281 gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac
     5341 ctgtacacct gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc
     5401 atcctttggt tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga
     5461 aatacttcga cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca
     5521 aagcgatgta gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac
     5581 acctgaattt accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc
     5641 taccaatgaa ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag
     5701 acaaatctac cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga
     5761 atttaaggga ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta
     5821 ttatgctgcc atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta
     5881 ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc
     5941 accatcccct atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct
     6001 aaatatattt taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa
     6061 aaaatcaatc atttttgaat ctttgctttt cattgcattg tcaagcatag tgtccccact
     6121 atttgctttt tgaaattttt tgtttacatc aaccaatcca ttattgataa aatattctag
     6181 cactttatag ttggcaccat ctttactaag taagtattca acatcataca gattacaata
     6241 tctattgttt ttcattttct ccatctctgc gacatcttgt tttgtaaaat taaatttaga
     6301 tagtaaataa agtgctatat cagctttttt attgcctaaa gaataagcaa gggcctgatg
     6361 agccattttt ttatcagtca ctttattgtt aatctcttca taattagatg ctttaatcgc
     6421 atcctccaaa gatatgtctc tgagttttat ttttttgaag ttatctgtac ttagcattct
     6481 tggtatatga tactcaagct gaaacacaaa gtttataatt ctatccagac taacaggatc
     6541 aatatttttg ttataacaaa actctctgaa tccttgatct ttactctttc ttataaaatc
     6601 aataagatgc aacccaattc caagcctcat atctttagtg ttaaatattg gaacatcagt
     6661 ttt
//
LOCUS       DAJVPT010000165         6636 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVPT010000165 DAJVPT010000000
VERSION     DAJVPT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(286..1188)
                     /locus_tag="OQM47_003691"
     CDS             complement(286..1188)
                     /locus_tag="OQM47_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCS1481685.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1267..2220)
                     /gene="lsrR"
                     /locus_tag="OQM47_003692"
     CDS             complement(1267..2220)
                     /gene="lsrR"
                     /locus_tag="OQM47_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCS1481686.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2469..4004
                     /gene="lsrA"
                     /locus_tag="OQM47_003693"
     CDS             2469..4004
                     /gene="lsrA"
                     /locus_tag="OQM47_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS1481687.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3998..5026
                     /gene="lsrC"
                     /locus_tag="OQM47_003694"
     CDS             3998..5026
                     /gene="lsrC"
                     /locus_tag="OQM47_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCS1481688.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5026..6018
                     /gene="lsrD"
                     /locus_tag="OQM47_003695"
     CDS             5026..6018
                     /gene="lsrD"
                     /locus_tag="OQM47_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCS1481689.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6030..6636
                     /gene="lsrB"
                     /locus_tag="OQM47_003696"
                     /pseudo
     CDS             6030..6636
                     /gene="lsrB"
                     /locus_tag="OQM47_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1512 a         1697 c         1733 g         1694 t
ORIGIN      
        1 agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc aggctatttc
       61 tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc tgaacgaata
      121 acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg
      181 cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata gacatgtccg
      241 cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg acggcatacg
      301 gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct tattatccgt
      361 gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc caaggcaacc
      421 aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac cgcagagttc
      481 cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag gagaaagaat
      541 atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg tggttagatc
      601 aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc tgagcatata
      661 ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat cggaacgatg
      721 gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc cggtcgctcg
      781 ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa cttcgcgtgc
      841 cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt tatataaaac
      901 aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt ctatgccggc
      961 gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt tgagatcaaa
     1021 ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct gtcccactgc
     1081 tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc ctgcatccag
     1141 cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta gattatcctc
     1201 ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta ataaggtcat
     1261 gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca gtgcgttgat
     1321 ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc ctgccacgcc
     1381 aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt cgttatgtat
     1441 tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa tgtcgccaac
     1501 cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg agcgaatgat
     1561 tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat ccgctgcttg
     1621 cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg caatgtcagc
     1681 ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt taagctgccc
     1741 gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa tttgctgtga
     1801 cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa aaccaatcgc
     1861 cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta tccctagtcg
     1921 cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca gcgaaaactg
     1981 acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa tctgtacgcg
     2041 aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac gggtcaggcc
     2101 gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc acgcgatccg
     2161 cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt tgattgtcat
     2221 aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag ttcttgcgca
     2281 tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac gcaaccgtga
     2341 aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg tccacctaag
     2401 gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca aaactgaacg
     2461 ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc cgctcggttt
     2521 ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg catcaggggg
     2581 aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg aagattattg
     2641 ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac tacgccagat
     2701 taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa ccgctgcttt
     2761 tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa cagctctcca
     2821 tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg catagtctgg
     2881 caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg ctgatgcgcg
     2941 actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg gaaaccgaac
     3001 gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt tttatctcgc
     3061 ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc gacggaacca
     3121 tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag gccatcaccc
     3181 cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag ttacctggta
     3241 accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg actggcgaag
     3301 gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg gctgggctgg
     3361 tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact ttgcgtggcg
     3421 gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa cgtttactgc
     3481 gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc gatgcttcgc
     3541 tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg aaaaccgcga
     3601 aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt aaccaaccgg
     3661 aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt gccaaatgcc
     3721 tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg gatgtctcgg
     3781 cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg gctgtgctgt
     3841 ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat gtgatgcatc
     3901 agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag actattatgc
     3961 gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta ttcagaacaa
     4021 ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg gttttctcga
     4081 ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc aaatcctgat
     4141 cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg tttcagtggg
     4201 ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag gatattcact
     4261 acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt tcaacggtgc
     4321 cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt tagggttgta
     4381 cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac ccgccgaact
     4441 gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt tgacgataat
     4501 tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca gtttttatgc
     4561 cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag ccattcgcat
     4621 tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt ttgcttcgca
     4681 gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa ttgcagcctg
     4741 cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg cggtactcgg
     4801 cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc cggcatggtg
     4861 gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg gacgcctgcg
     4921 ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga cgccaccgcc
     4981 atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg tattcgctac
     5041 ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt tggtgcaatt
     5101 aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt tatctgcatt
     5161 ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga tatttcgttt
     5221 ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag tggtgtgccg
     5281 atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct gatcaacgcc
     5341 ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac gctgtatctg
     5401 tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta cgaaggtatt
     5461 ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg actccccgtt
     5521 ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca taaaacccat
     5581 gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta tagcgcgatt
     5641 ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc tgcggtcgcc
     5701 gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc gtcgtttctg
     5761 atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg ttccggttcc
     5821 attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg tttgcaaatg
     5881 gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat cgtcgttgtc
     5941 gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc gcgtcgggcc
     6001 aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag aaaatcgcct
     6061 tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca gagcgtattg
     6121 catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac ggcgcacaac
     6181 aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa cccagtgttt
     6241 ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc attattcgtt
     6301 tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg
     6361 agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta cattaatcag
     6421 ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa
     6481 gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca aaaccagtgg
     6541 gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat tgtcactacg
     6601 cagtttggct ataacgatgc cactaaatcg ttacaa
//
LOCUS       DAJVPT010000166         6612 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVPT010000166 DAJVPT010000000
VERSION     DAJVPT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            230..2662
                     /locus_tag="OQM47_003697"
     CDS             230..2662
                     /locus_tag="OQM47_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1481690.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2729..4198
                     /locus_tag="OQM47_003698"
     CDS             2729..4198
                     /locus_tag="OQM47_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1481691.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4198..5859
                     /locus_tag="OQM47_003699"
     CDS             4198..5859
                     /locus_tag="OQM47_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1481692.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6080..6421
                     /gene="symE"
                     /locus_tag="OQM47_003700"
     CDS             6080..6421
                     /gene="symE"
                     /locus_tag="OQM47_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1481693.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT         1953 a         1566 c         1521 g         1572 t
ORIGIN      
        1 gaaagaaacg caactataag atttttatat attttgttag agttgatatg gttcctgtaa
       61 cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata acagagatat
      121 caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat agccaggcta
      181 ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca tggcggaact
      241 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
      301 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      361 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      421 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      481 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      541 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      601 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      661 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      721 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      781 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      841 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      901 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      961 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
     1021 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
     1081 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
     1141 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
     1201 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1261 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1321 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1381 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1441 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1501 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1561 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1621 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1681 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1741 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1801 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1861 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1921 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1981 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     2041 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     2101 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     2161 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     2221 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga
     2281 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2341 cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2401 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2461 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2521 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2581 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2641 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2701 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2761 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2821 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2881 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2941 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     3001 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     3061 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     3121 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     3181 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3241 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3301 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3361 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3421 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3481 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3541 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3601 gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag tactggcgaa
     3661 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3721 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3781 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3841 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3901 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3961 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     4021 cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     4081 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     4141 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg
     4201 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4261 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4321 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4381 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4441 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4501 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4561 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4621 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4681 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4741 ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4801 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4861 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4921 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4981 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     5041 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     5101 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     5161 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     5221 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5281 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5341 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5401 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5461 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5521 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5581 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5641 gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag tttttacaat
     5701 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5761 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5821 catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc tcacaaaacc
     5881 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5941 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatgtatcc
     6001 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     6061 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     6121 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     6181 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6241 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6301 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6361 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6421 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6481 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg
     6541 aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt tgtcacatct
     6601 ttggcaatac ct
//
LOCUS       DAJVPT010000167         6352 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVPT010000167 DAJVPT010000000
VERSION     DAJVPT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..146
                     /locus_tag="OQM47_003701"
                     /pseudo
     CDS             1..146
                     /locus_tag="OQM47_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            671..1849
                     /gene="ltrA"
                     /locus_tag="OQM47_003702"
     CDS             671..1849
                     /gene="ltrA"
                     /locus_tag="OQM47_003702"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1481694.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1932..2111
                     /locus_tag="OQM47_003703"
                     /pseudo
     CDS             1932..2111
                     /locus_tag="OQM47_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2141..2623)
                     /locus_tag="OQM47_003704"
                     /pseudo
     CDS             complement(2141..2623)
                     /locus_tag="OQM47_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2879..3859)
                     /locus_tag="OQM47_003705"
     CDS             complement(2879..3859)
                     /locus_tag="OQM47_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1481695.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3859..5058)
                     /locus_tag="OQM47_003706"
     CDS             complement(3859..5058)
                     /locus_tag="OQM47_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1481696.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5380..5718)
                     /locus_tag="OQM47_003707"
                     /pseudo
     CDS             complement(5380..5718)
                     /locus_tag="OQM47_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5785..>6352)
                     /gene="istB"
                     /locus_tag="OQM47_003708"
     CDS             complement(5785..>6352)
                     /gene="istB"
                     /locus_tag="OQM47_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1481697.1"
                     /translation="KTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGK
                     THLAIAMGYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEI
                     GYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHH
                     SHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1712 a         1467 c         1514 g         1659 t
ORIGIN      
        1 acatcctgcg ccagtatgtg ctgatgcccg gtaaagtcca tgccgatgat atccccgttc
       61 cggtccagga gccgggcagc ggtaaaacaa ggactgcccg gctgtgggtc tacgttcgtg
      121 atgaccgtaa cgccggttcg gaaattgcag cataagttat ccatgctctg aggtgggtag
      181 aggtgggccc tccggtcagg gtttacagga gcacagacca aacgacccgg tgctgcttca
      241 ttctaaggaa tgaggtggtg agcgactcgg gaaaaggcag agatctactg tcacgtgggt
      301 tcgggaagct gaacgaaagt aaactgtccg atgaagcatc gaaatcttag atgatgccaa
      361 aatggggccg tacagccgtc ccctgataag cgcagaagac acctgcttac tgactgcgcg
      421 gcatccggtg tgaaggcagc aggatcccgg gccagaccat ctcagggaac gtgagaacct
      481 gtttccggca cgtatcggga aacaggggcg gacccattcg tagtagtgat gaaagatctg
      541 taatgggtct ggagcgaagg gatggggatc acattcattg cagaagacac aacccaaaag
      601 gggatgacgt aactgatgca acgaaaatca tttgaaatac caaaagcact ggtctgggca
      661 tcatatctgg atgtgcgtcg caacaaggga gccccccgga tgcgacgggc agactttgaa
      721 aatgttcgac cagcaacgtg atggcaatct atacaagatc tggaaccggc tatgttcggg
      781 aacatggttt ccgccaccag tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg
      841 cattctgggg atcccaacag tatccgatcg aattgctcag ggagcgataa aacttttcat
      901 ggaagaaaag cttgacccga ttttccatgc tgattcatat ggttaccgac caggcaaatc
      961 agctcatgac gctctgaaac aatgtgccat ccggtgctgg cgttatagct ggatactgga
     1021 agtggacatc agtgcgtttt tcgaccatgt gagacatgat ctggtgctca aagctctgga
     1081 acaccacggg atgcctaaat gggtcatcct gtactgtcga cgctggatgg aagcgccaat
     1141 gcagagttgt gaaaatggag aattaataac ccgaacacga ggcactccgc agggcggggt
     1201 cattagtccg ctacttgcta acttgttcct tcactatgca tttgatttgt ggatggaaag
     1261 agaatatcgg ggggtaccgt ttgagaggta cgctgacgat attgtagtac attgttcacg
     1321 aatgagtgat gcaacaaggc taaagaacag gttatcggag cgcttctcgg aagtcgggct
     1381 ggtcctgaat gcagggaaaa cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt
     1441 cgcaacgagt ttcacctttc tcggatatga cttcaaagtg cgtacgctga agaatttcaa
     1501 cggcgaacgg taccgaaaat gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac
     1561 agaaacaatc aagaagtggc gtatacatcg ctcaacagct gagagtttgc tggattttgc
     1621 gaggcgctac aatgcgatag tgagaggctg gatcgagtac tacggaaagt tctggtccag
     1681 aaatttcaac tatcgactgt ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc
     1741 taaatacaga ctttcgaacc ggaaggctca gcgaaagctg acgctggtaa ggaaggagta
     1801 tccgaagcta tttgttcact ggtatttact acgtgcatca aatgagtgat caagagccgt
     1861 atgacgcgag agtgtcacgt acggttccgt gagcagccgg ggagtgaaat tctctccggc
     1921 tgactcgact cgcgggttcc gacagcgatg acgaacatgc ggcggtgttg tactcgctga
     1981 tcggcacatg ccgtctgaac aatgtggaac cagaaaaatg gctgcgttac gtcattgagc
     2041 atatccagga ctggccggca aaccgggtac gtgatctgtt gccctggaaa gttgatctga
     2101 gctctcagta aatatcaata cggttctgac gagccgctta cttttggcgt aattctgctg
     2161 cgcctttact gaacacaatg ccgtattcag caagaagtcc acgaatgtga tttatcagtt
     2221 gtgtcctttc cattgaaacc aaccgacgca tattcaaaaa agtctggacg tcctgttgct
     2281 ccagagtttt tattggtacg ggtcgtatag ttccatgttg gcaggcctca gctattgcct
     2341 gtgcgtcgtt ataatcattt ttctggcacc gctgataagc tttaacatgt tgtgccggaa
     2401 gcaatattac atcatggtcg agtttcctga tttcccgggc ccagtaatga gatgccccgc
     2461 cagcctccat cgcaacagtc attttaggct gttgcaccag ccagttaagc agttgattgc
     2521 gagaaagttt ggtctttcct gcgggtttgc catgagcatt gatactgaaa agataaaagt
     2581 tcgttttagc cagatcaatg ccggtgattt tctgttcaga catggtatcc cgcttacttt
     2641 tgttgttaaa gtttatccat catggcgcaa ttgaaggctg ccgtgaaaga gggggaagac
     2701 tatctcatcg gttactgtta aacggctaag agaaactatc tttttcaagc aaagaagata
     2761 cggtgaaaca ggcgatttca ccgtgaaata ctgttcaaaa aaaacgctaa taaaagcaaa
     2821 atttctttta aaacagccaa ttaaaaactg gcgagttgct ggcgaaaaac ttgaattttc
     2881 acagcgaatt cagattatct tttagtacaa gagcaatcat acgatctaac ttatcctgaa
     2941 cttccaacgg taatctgccg aattcatatg agaacgtacg tccctttact cgttttcgag
     3001 caaatttatc tttactatca aatttccata acaaagttac aaccgcttta tcctttactc
     3061 ctgaatcaag caatgaagac atttccttgg ttataagccg cataatactg tttttaacct
     3121 cgtcttcaga aagattgtca tcagcagtaa taagctcaat actcggcgag atattcttca
     3181 caacttcatc aatagatata tcacccagcc tcagtcgttc acttaattca gccagttgcc
     3241 tgtaatcggc gtatgtcagc tcagactgaa caggaaaaag agacacaaaa tctttaggta
     3301 cggacgctgc ttggagggca cgagtcacct tagctgccga caatccctct ctttccgcaa
     3361 tttgcttctg gttcataccg gcctctttga gacgtactaa acgcaaccca atttcacgaa
     3421 tattatgttc aagtgacgtc tgcaaatctt tcgccagatg ctgagcttca gaaacagtaa
     3481 gctcatcatc cgttacaagt acacgtaatc caaccttgca tagcaaagct gctgcccgac
     3541 gacgggaacc atccaaaatc tcaataagat cgccagtccg aatcccaata catggataga
     3601 attgttgtaa acgtatagtt ctggtgatgt cttttagtga gtccggcgtc agggctgtct
     3661 gttcgcggcc attcacctct tcaaccacaa aagtttgagt atccactttg tcatgatcaa
     3721 ccttgatctc agtaaatttt gcctttcggc cagtattgag ggtaaataca tgaactggag
     3781 ccgatatttc ttcagtatta ttcaggatat ttgtattcag cgtacggcca atagtcggtc
     3841 ggtgctttct gttttccatc agtagttggc ccttataaat tcaatacgat caaaaactgc
     3901 tttagcgaaa tcttctgctg ccatacgtgc atttttcaaa gcttcaccac tgccaacata
     3961 agtaacagga ttcgcagata tcacggtatc aaacgactcg ccgcttctct caaaaccatc
     4021 aagacgtggc attgaaacat caagcatgtc accaccgaaa atctcttttg tcaggctatg
     4081 gcagtattta tgatctgact tattcgccag tttcgacatg aagccaatat ttgcctgcaa
     4141 acggcatgaa catccatcct gttcaataat ctgtacaagt tcaggtaaac gagccaaata
     4201 tttaagcgtg gaatggaaat caacttgtgc tggcggcacc ggtgtaaaca tgatatctgc
     4261 ggcagcaatg gcatttttca aaaatgcgtc cagatgcggg cctgtatcaa taaggatgaa
     4321 gtcgaaatca tgcttcagct tatcaataat attctcacgc aatatcgcgt gtttgttttg
     4381 tcctaataga tgctcctcgc acagagtatc ccagtttgat gcaataaagg cgtcgtcaat
     4441 agaagccggc ataacataaa cgccagggat aactgacgga acgataaaat cttccagtaa
     4501 ttcctcacga ctcacgttct gcaacatagc ctgcggtgcg gttgtatcaa ccagccccac
     4561 cgcatgcaag tagttcagaa acatagtggc tgatgactgg ggatctaaat caaggagcag
     4621 aatacgtaaa tcttctgaca gcaggtgagg atgagcacgc agggcgtgag ctacggaaac
     4681 actactcacg gttttagaca caccgccttt tagactaccg atgaatatgg aatatgcctc
     4741 actataacga tcgcgatatt taggaatgcc acgatgtgca tagatatcga taatgttctg
     4801 gatggttaat gcatattttg tcgcggttcc tgctgggcgt ttttcaaact gatagccctg
     4861 agcctccatc tcaccgactg caccatccac gcttgcgcgc gttaattttg gaagcttagc
     4921 tacagcagac tttgaataga cttgataaaa aacatcagcc tgaaactcct gcttttgctc
     4981 ctgaatctgt tttgttaggg cgagcaacat tttatctgca cgctgcgcaa ccttacttaa
     5041 ctgctcaaaa ctagtcattc cccctccgtg ctatgtagga ttttaactta ttacaaaaaa
     5101 aactgcatta gcagggtaat gcatgcaagt acatattgta catgcttata ttaaaaatgc
     5161 gcgcattgct gatttattaa tcatcggaat gaatattact gtaagatagt atgcctttcc
     5221 gggagaaaga cgctgatttc ccccgaataa tccagttcac caccacactc cggacaacag
     5281 ctttcttcag gctccagatg gtgtatttcg cgggaaacat gttccggtaa cggacggcga
     5341 tgacgtgact gtcgcaacag gcgggggtag tgcttccaat cagtagaaca tgtgcttttc
     5401 aatgaaggag ccgatgactt tttcgtggat ttccacggaa cgcgagaagc agattgtttt
     5461 acgtgccagc cgcttgatgc gggtgcggag ggtccggttg ttacgtgcaa tacgttgcgt
     5521 gaagattttg ccggtcaggt gtttctcctt cggcacttct ctggcgtagc tgccccagtc
     5581 atcgctggtc accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt
     5641 ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag tgttgtacgc
     5701 ataccagagc cagtgttgtg tcaacgacgg atgaaaagtg atccacttat atctccacca
     5761 acggcccaat attgatccac cgttttactc aggattagct tctgctataa ccccggcctt
     5821 tcgtttctgt ctgagtcgat agctttctcc tttgatttga acgacatgtg agtggtgtaa
     5881 gatacggccc agcatcgctg aggtcagtgc tgcatcaccg gcgaacgttt gatcccactg
     5941 cccgaacggt agattggatg tcaggatcat tgcgctcttt tcgtaacgtt tagcgatgac
     6001 ctggaagaac agttttgctt cttcctgact gaacggcaga tagcctattt catcaatgat
     6061 gagcaggcgg ggggccatta ctccacgctg aagcgtcgtt ttataacggc cctgacgttg
     6121 tgccgtagat aactgaagta acagatctgc tgctgttgtg aagcgaactt tgatacctac
     6181 acggactgct tcatagccca tcgctattgc cagatgggtt ttccccacac ctgatggccc
     6241 cagtaatacg atattttcat tacgttctat gaagctgagt gagcgtaacg actggagttg
     6301 cttctgcggt gctccggtgg cgaatgtgaa gtcatactct tcgaacgttt tc
//
LOCUS       DAJVPT010000168         6345 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVPT010000168 DAJVPT010000000
VERSION     DAJVPT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..117
                     /locus_tag="OQM47_003709"
     CDS             <1..117
                     /locus_tag="OQM47_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1481698.1"
                     /translation="MQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(456..1226)
                     /locus_tag="OQM47_003710"
     CDS             complement(456..1226)
                     /locus_tag="OQM47_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481699.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1271..1705)
                     /locus_tag="OQM47_003711"
                     /pseudo
     CDS             complement(1271..1705)
                     /locus_tag="OQM47_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1717..1938)
                     /locus_tag="OQM47_003712"
     CDS             complement(1717..1938)
                     /locus_tag="OQM47_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481700.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1939..2622)
                     /locus_tag="OQM47_003713"
     CDS             complement(1939..2622)
                     /locus_tag="OQM47_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1481701.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2686..2997)
                     /locus_tag="OQM47_003714"
     CDS             complement(2686..2997)
                     /locus_tag="OQM47_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481702.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(2994..3896)
                     /locus_tag="OQM47_003715"
     CDS             complement(2994..3896)
                     /locus_tag="OQM47_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCS1481703.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4336..5295
                     /locus_tag="OQM47_003716"
     CDS             4336..5295
                     /locus_tag="OQM47_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCS1481704.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5295..5690
                     /locus_tag="OQM47_003717"
     CDS             5295..5690
                     /locus_tag="OQM47_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1481705.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5873..6253
                     /locus_tag="OQM47_003718"
     CDS             5873..6253
                     /locus_tag="OQM47_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCS1481706.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1737 a         1653 c         1532 g         1423 t
ORIGIN      
        1 atgcagcttg aacgccagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttgc ctgcgctctg gcaaataaac tggcccgaat tgcctaggca
      121 atcactactc agcaaaaaga atatcaggcc tgatatatag atgctgacaa aactcaatta
      181 gcttattgca aacaataaga tacaagttac cgtctggttt tgcgaagatt gattattgat
      241 gacatgaacg gcaaatcgac ctgattaaaa acctaacagg ggaaaatggc tcactgaagc
      301 cgaggggatt tttaggatta tcaggtgcgg aactcatcga ggcgcgggca tcccttgccc
      361 acagagacgc cggatagatt taagcaagcc caccattaat caaaaatcag tgttgcaaaa
      421 acgggggtga ccatagattt tccccgccat cgtcatcatt tccggatttt tgccagggca
      481 tccgccatgc tgcggatttc ggcccgggct tcccggacaa cttcccgcag aacaccacgg
      541 tcaaaccacc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
      601 gacaggatat gctcaataca atcgctgacc agatacattt ccaccgccga aaaacgtgcg
      661 ccccggttca ggatggcatc gagcaccacg ggcaggtttt catcgtcttc atcgagccag
      721 caggggtgat cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagcaac
      781 gtctgaccgc ttgtggtcag cgccgcccgg atacgccgcg catcttctgc cccccggcaa
      841 ctgacactgc atactgcatc gcgcaccgca tcaagaactg ccctgatttc gtcttcggtc
      901 agccattcac cggcatacgg gaaagacacc tgcgttccgt ccgccggaag taccggcagc
      961 gactgccact gactgccgga cagaacaagt tccgctccgg ttccggtcag tttctgaatg
     1021 gcggaggcca ggtgcggcgg cagcacgcca tcgtggcggt taacgagtaa cgtcgccagc
     1081 cggggcgcat ccagccgcac gtaaatgctg atccagcccg gatagttcgg gttacattgg
     1141 tcaacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtgacgacaa
     1201 atcagagcat tcagcgtttc attcataatt catgaactcc ctgtatcgcc gcccacaggc
     1261 ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag caggctgcgg gcctgatcaa
     1321 ccatcgcctg tcggcgggta acgtattccg ggagggggaa accgtgatcc cgggcagccc
     1381 attcacgttt ccacagtgcc tgttcagcga tctgagtcag ggtctccagc agttcagcag
     1441 ggacggtcac ctggcggttt tcgcgcacgt tgctgataat ctccatcgcc gcggcggaag
     1501 aaataccgga ctcaatccgc tagtcttccg ggatgtcctc caggtgcgcc aggacggtcc
     1561 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctga atctcttccg
     1621 gggaccagat aaccagcatc agcggcgtgt ctcccgaata ctttgctgcc agcgcccggc
     1681 atagcgtttc gcatgttccg tacatactct ctcccctcaa tctttgaagc aggaatccag
     1741 ttccagaatt tcggtatatg cctccatcaa gaggcggttt gcgcgaaaca acgtctgacg
     1801 caccagacgt tccggcaggg tgtcagcatt aaagctgtac cgccgctcca tctcattcat
     1861 ggttgtacgc aggtcgcaca tggcattggc cacctccgcc acttcggcgc gaagtttttc
     1921 gtgtccggca tagttcattt acgcagcctc cggcataaac cagtcatcat tcgcggcccc
     1981 ctgctgcatg gcccgctgta cggcagccag tcgttgctgt ccggcacggt gatactgctc
     2041 aagcagctcg ataccgatat accggcgtcc ggactggagg gcggcgacgc aggttgaacc
     2101 gctgcctgca aacgggtcca gcactatcgc attcgggtgt gtgaagctct caatcagcgg
     2161 ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg ttgcccgaat atttccagcc
     2221 caacacgtcc ggcagcgggt tttgtggcag acgtggacgg ccttttgcca ggatgtaggc
     2281 gcattcgtgg cgatagccca catatgcggc cttcgatgtg tagtttttgg tgaagaccag
     2341 gtgaccaaca acgctgaatc ccgcattttt ccaggcagac ataaagcgat cgacgcggtt
     2401 ccagccatag aagctcacca tcagcgcgtc ttttttgagt acgcggtaca tttcattaca
     2461 ggccggttgc agccattcat cagttttatc accagcaatg gtacgcccct gacggtcacg
     2521 aaaaccgacg agatatggcg gatcggtgag gatgaaatca atggcgttat caggaatacg
     2581 ggccataaca tcgatgcagt taccgaggac aaaacgggac ataacagatc cttctcttac
     2641 agagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcatc gcttccggta
     2701 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tttgacagca aggttatgcc
     2761 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac gggctggcgt acactgtcac
     2821 cgacaccagc cgggagcggt aaaagcgccc gtcccgtgcc cgccggacgc gggacactga
     2881 ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag gagcacggcg
     2941 acatcctttg tcggaaaaga aacgggcggt tgcccgcccg tctccgtcat acttcatccg
     3001 ccataatgcg ccacattgtc gactatccac gcaggtccgg tgacttccgg ctgctcatcg
     3061 tcatctgtcg gggaagacca ttcaagttcc ccccacagca cattgacgag ctctccacgc
     3121 tcatacaact gccagccaca gaaatcacag ccctgttcgg cataccagtg ctccagcgtg
     3181 cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt ccggctgaca ccacggagtg
     3241 tcgaaatcca cctgaatgaa gttttctccc tgactgctga tattcaggtc atatccacaa
     3301 ggccacttca cgccataccg ttccgtatac cagtgatatg cagaaggaac accgttcaga
     3361 acaccaccat taaagccgtt cacttcaaca tccaggcggg tcggcagaac cgtcagcagg
     3421 tcaaacggca tggttttttc cggcactacg ttgtcacaca gacggttcca ccacaccgat
     3481 acatcctcgt taccccatat gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg
     3541 atggtttccc tggcatcatc cggtaatcct tcccaacgac gctggccagt accgctctgt
     3601 aaccagagtt catgcagcat caggcagttt tgttcatcca gcaacacacc gttctgcagg
     3661 tgtgtcagcc agcgggtgaa cgcgatattt tccgtcgata cagcaccacg tccggcagcc
     3721 gtcactccgg ggcactgttc agagcggata ttttcagtga tctgcagaag tccggcactt
     3781 ccggccagaa acagctgaat accttcattt gtggcgcggc gataaagcgg tgtgactgca
     3841 ccgctggcca gtcgtttaat ctcagcgatc tgtgccggct caccaggaaa atacatacga
     3901 ttcgagcacc agtttggcat gggaattctc ctctcacatg cggcctccat caggccgtgc
     3961 cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt
     4021 ggaggaggaa tgacgaaacg tgaccgggtg aggaggccgg cgacagcgac acacttgcat
     4081 cggatgcagc ccggttaacg tgccggcacg gcctggttaa atagtatccg tctcagttcg
     4141 tggcgcaaaa tgagaaacca ataagaactc aaaatgaacc acaacaagaa cataaagaga
     4201 ctctaaaaga gcaacaccgt ttgacataga ctcaaaatga gtcacaataa gaaacaataa
     4261 aaaactcaaa aagatgcacc gacataaagg atgctcaatt tgagataaac acaaactcaa
     4321 aacgaggcaa aaagaatgct aaaagtatca tgcgatgatg gttcaactaa cgtaaaactg
     4381 gcctggcttg aagatggcga ggtaagaacc agtctgtcag gaaacagctt taaagaaggc
     4441 tggaacccgg gactgtttaa tgccggcaaa gtttacaatt acgtcgtgga tgaaaaaaaa
     4501 tacacctatg atttgggaag cactgctgtc ataggtacaa cacatgtcag ttatcaatac
     4561 agcaccacca atctgcttgc catacatcat gcactgctga catccggcct tcagccacag
     4621 gatgtagaac tgacagtaac attacctgtg accgaattct ttgataatga caatcaacca
     4681 aatgaagaaa gaatagaaag aaaaaaggca aatgttctcc gtgagatatc tctgaataaa
     4741 ggggaaacat ttaaaattaa aaaagttaat gtaatgcctg aatctttgcc agctgcattt
     4801 gaatcactaa aaaaagataa agtaaacaaa cttgaacgca gcctgattat cgatcttgga
     4861 ggaaccactc tggattgtgg tttaattctc ggtgcatttg aaggcatttc tgaaatccgt
     4921 ggctattcag aaatcgggac atcccgcatc actcataccg tcatgaatgc gctgacgaaa
     4981 gcctccacac catgtaatta ttttattgct gatgagttaa taaaaaacag acacgataat
     5041 gaatatctcc agacattaat aaatgatgta gctgaaataa aaaacatttc tcatgtaata
     5101 gacagagaag tgaaatctct ggcagaaagc atcaggcagg aaatttcaac attctcagga
     5161 atgaaccgaa tctatctcac tggcggagga gccgagctta tttaccctca cataaagcaa
     5221 tatttcccga atctgaaagt gaacaaggtg gatgaacctc agtttgcatt ggtgaaagca
     5281 atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag aaagaaagtt gtggcatacc
     5341 tgcacccggc actttatccg caggacaacc tgacacaaca aacaatagac tctctcccag
     5401 tacaaatgcg gggagatttc tacagacaat cccttatttg cggagcagct ctttattctg
     5461 ttgccccccg actgttaacg ctaatcagcg tttttttcag tgagaaaatc actgcagaaa
     5521 atctggtgaa acttatagaa caaacaacag gatacacatc cacatcaatt gatataagtg
     5581 tgttaaaaaa catcatagag gcatcttcag aaaacaaatc agagagcatc acttcaaaag
     5641 atgatttcga agaacaaacc agacgcaatc tttctatgtt aaaaaaataa taaaagaaga
     5701 gcaggttcag tcaattacct gctcttcttt tatacatact aaaaaatcag atttttacga
     5761 atattcagat tatcataaaa aatgcaacag acaggaatgc aataattttg cattatctcg
     5821 ttgcttgtaa tgcaaatatg cattattgtc agtgtgtgac aataacagag agatgacgat
     5881 ggcgaccatt aacgcacgta ttgatgatga tataaagaac caagcagatg aagtgctgaa
     5941 gctgatgaat atcagtcaaa ctcaggctat tgcagctttt tatcagtata ttactgaaca
     6001 aaagaaactg ccatttgtga tcacctcaat tgtaaaaacg ccgcatgatt tattacgtga
     6061 aagtacagac atgttagctg aagctcttgc agtgatttcc aatctacagg tgtggactga
     6121 acagcaggat gggattggaa aagcaaagct aatggagtat taccgccgcc tcgacgcact
     6181 gtactgttgt gcaaaagaga aaataggtct tctttcagat aaccgggatg ctgaactagg
     6241 ctgtgtccct taatcatctg agttatagta actgtctgtt tacacaacat attgaactat
     6301 ggctcgctac gaccttcccg atgaggcatg gaccatcatc aagcc
//
LOCUS       DAJVPT010000169         6255 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVPT010000169 DAJVPT010000000
VERSION     DAJVPT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQM47_003719"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQM47_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1481707.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQM47_003720"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQM47_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1481708.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQM47_003721"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQM47_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OQM47_003722"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OQM47_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1458 c         1476 g         1662 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgat
//
LOCUS       DAJVPT010000170         6205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVPT010000170 DAJVPT010000000
VERSION     DAJVPT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            62..1522
                     /locus_tag="OQM47_003723"
     CDS             62..1522
                     /locus_tag="OQM47_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1481709.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1558..1761)
                     /gene="ydfZ"
                     /locus_tag="OQM47_003724"
     CDS             complement(1558..1761)
                     /gene="ydfZ"
                     /locus_tag="OQM47_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1481710.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1939..2625)
                     /gene="ydfH"
                     /locus_tag="OQM47_003725"
     CDS             complement(1939..2625)
                     /gene="ydfH"
                     /locus_tag="OQM47_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1481711.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2714..3460)
                     /gene="ydfG"
                     /locus_tag="OQM47_003726"
     CDS             complement(2714..3460)
                     /gene="ydfG"
                     /locus_tag="OQM47_003726"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1481712.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3597..5642
                     /gene="dcp"
                     /locus_tag="OQM47_003727"
     CDS             3597..5642
                     /gene="dcp"
                     /locus_tag="OQM47_003727"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1481713.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5687..6205)
                     /locus_tag="OQM47_003728"
     CDS             complement(5687..6205)
                     /locus_tag="OQM47_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1481714.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1544 a         1489 c         1596 g         1576 t
ORIGIN      
        1 ggggattttt attcatataa caaaacatat agcttgccat atttaaattt aaggaattgt
       61 tatgggaaat aatttgttat cagcaaaagc gacactccct gtttatgatc gtaataacct
      121 ggccccaaga attgttcatt taggctttgg tgcatttcac cgtgcgcatc agggcgtgta
      181 tgccgatatt cttgctacag aacatttcag tgactgggga tattatgagg tcaacttaat
      241 cggcggcgaa cagcaaattg ccgatttaca acagcaagat aatctttata ccgttgcgga
      301 aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag ccttgcacgt
      361 acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa tcgcgattgt
      421 ctctctgaca atcaccgaaa aagggtattt ccactctccg gcgaccggac agttaatgct
      481 cgagcacccg atggtcgctg ccgacgtgca aaatccccac cagccgaaaa cagcaacagg
      541 ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat ttaccgtcat
      601 gtcatgtgac aacatgccag aaaacggtca tgttatgcgt gacgttgtca cttcctacgc
      661 gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat aacgtgactt tcccatcaac
      721 aatggtggac cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa tcgaacaact
      781 taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct ttccgccagt gggttatcga
      841 agataacttt gtagccggac gtccggaatg ggaaaaagcg ggagccgaac tggttagtga
      901 tgtgctgcct tatgaagaga tgaagttgcg catgctcaac ggcagtcatt cattcctggc
      961 atatctgggt tatcttgctg gatatcagca cattaatgac tgtatggaag atgaacatta
     1021 tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg ccgacgctga aagtgcaggg
     1081 cgttgatttg caagattacg ctaaccgatt aattgcacgc tatagcaacc cggcgttacg
     1141 tcatcgaacc tggcagattg cgatggatgg cagccagaaa ttgccacagc ggatgttgga
     1201 ttctgttcgc tggcatctgg cgcatgacag caagttcgat ctgctggcgc tgggcgtcgc
     1261 gggttggatg cgttatgtcg gtggtgttga tgaacaggga aatccgatag aaatcagtga
     1321 cccactgtta cctgttattc agaaggctgt acaaagtagt gccgaaggga aagcgcgcgt
     1381 ccagtcattg ctggcgatta aggcaatctt tggtgatgat ttgccagaca atagcttgtt
     1441 tactgcaaaa gtgacggaag cgtacttgtc tttattagcg catggcgcga aagcgaccgt
     1501 ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat cggcggcatt cacacactta
     1561 gttcatgccg agacgaatca ggtccagagg tgccagtttc tcttcacaac cttcaacaac
     1621 tacggttttt ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa tcgacaggat
     1681 cttgccagtg tgaccggtgc cgctaaccat aacacggctg ccagtggtga ttgcgttacg
     1741 gttacgatcg taggtcgtca taggtgtttt ctcctttctg atttacagtc gaagcccacg
     1801 taacgggcgc aatataaata ctcctgtcga gtgaaatatt ttttgttttt gatcaagctc
     1861 acatcttttt tgatgcatgc atgagccgta aggcgggggg aaaagcctct catcccggat
     1921 gggatgagag taaatgaatt actcttcgct aaaccagtcg ctgttttcct ggcggatctg
     1981 gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt gcacgttcta ccgcatcgcc
     2041 atcacgtttt tgcagggcag agaaaatatc aagatgctgg cgtaacagca tttctggtgg
     2101 agaaacatgg tcgaaactca tatagcgcac gcgatcaacg gtcgctttca ggttctcaat
     2161 ggtatcccac gccagttgac agtcggcaat ctgcgtcagg agttgatgga agttgtcatc
     2221 aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca atgcgttgct ggtgaagatt
     2281 ttgttccagt tggtagcact ggctttcggt aatcatgctc gccgcccgac gcgccaccgc
     2341 gcactcaatg gcctgacgga taaaactgcc gttgcgcacc tgggccatgg aaattttgtt
     2401 gacgtagctg ccacgctgcg gacgaatttg aatcaggccg ttttccgcca gtttaataaa
     2461 ggcttcacga accggctggc gtgacacatt gaaacgaaca gaaacttctt tttccgacaa
     2521 cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga cgaagaatac gataaatctg
     2581 ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg gtcattcgtt cttgcttgtg
     2641 agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa gcaataaccc ggcagttacg
     2701 ccgggtataa aaattactga cggtggacat tcagtccggc atagctttgg gtaaccggca
     2761 tcatttccag ggtattgata ttgacgtgag caggcagcgt tgacacccac cagacggctt
     2821 cgctgacatc ttctggcgtc aatgcaacgg tattttgata ggttttttcc gctttaccgt
     2881 catcgccttt aaagcggaca ttggaaaact cggtgccacc caccagaccc ggttcgatgt
     2941 cggtgacgcg caccgccgta ccatgcagat ccgtacgcag attcaggcta aactgacgaa
     3001 caaacgcttt cgtcgcaccg taaacgttac caccggcata cggccagctg cctgccgttg
     3061 agccaatgtt aataatatga ccatgattac gttcaaccat acccggtaag acggcgcgcg
     3121 tcatatatac taggcctttg ttgttggtat caatcatcgt ttcccagtct tcaacgctgg
     3181 ctttatgcgc aggctccatg cccaacgcca ggccggcatt atttaccagg atatcaatat
     3241 tgctccactc ggcagggagc gatgccagca tctcttcaat agcggcgcgg ttgcgaacgt
     3301 ccagttgggc gatatacaga ttatctccca gttcgtctgt taactcctgc aaccgctcct
     3361 ggcgacggcc agtggcgata actttatgcc cttgttgaat aaaacgacga gtaatgcatt
     3421 caccaaaacc tgccgttgct ccagttacta aaacgatcat ctcactgttc ctcaacgctt
     3481 ttgtgtgtca ataacatagc acgtgatgct taagagcggg taatactggt ttgcaagatt
     3541 gcactccgtt atcagtaagc ctacgctatg gaacacctcg cgttcaggag aagaaaatga
     3601 caacaatgaa tcctttcctt gtgcaaagca cactgccgta tctggctcct cattttgatc
     3661 aaattgccaa tcatcactat cgcccggcat tcgatgaggg aatgcaacaa aagcgggcag
     3721 aaattgctgc catcgcgctt aacccgcaaa cgcctgattt caacaatact attctggcac
     3781 tggaacaaag cggagaatta attacccgcg ttaccagcgt cttttttgcg atgactgcgg
     3841 cgcataccaa tgatgaatta cagcgtcttg atgaacagtt ttccgctgaa ctggcggaac
     3901 tggctaatga tatctatctg aacggtgaat tattcgcgcg ggtagatgct gtctggcagc
     3961 gccgtgaatc cctggggctt gatagtgaat ccatccgcct ggtggaggtt attcatcaac
     4021 gttttgtcct tgccggagcc aaacttgcac aagctgataa agcaaaatta aaagtactga
     4081 atacagaagc tgcgaccctg accagccagt ttaaccagcg gttacttgca gcaaataaat
     4141 ccggcggtct ggttgtgaac gatatcgcgc agctggcagg aatgagtgag caagagattg
     4201 cgctggcggc agaggcggct cgcgagaaag gtctggataa caaatggctg attccgctgc
     4261 tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga tcgtgcgacg cgtgaaaaac
     4321 tgtttattgc gggctggacg cgagcggaaa aaaatgatgc caatgatacc cgcgctatca
     4381 ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact gcttggtttt cctcattatg
     4441 ccgcatggaa aatcgccgat cagatggcaa aaacgccaga agcagcactc aactttatgc
     4501 gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga attagcctcc atacaggcgg
     4561 ttatcgataa gcagcagggc gggtttagcg cgcagccgtg ggactgggca ttttatgcgg
     4621 aacaggtacg gcgggagaaa tttgatcttg atgaggcgca gctcaagcca tattttgaat
     4681 taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa tcagctcttc ggtattaagt
     4741 ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt tcgtgtgtgg gaaatttttg
     4801 atcataatgg cgtgggactg gcgttatttt acggtgattt cttcgcccgt gattcaaaaa
     4861 gcggcggtgc atggatgggc aattttgttg agcaatcaac gcttaatgaa acgcatccgg
     4921 taatttataa cgtctgcaat tatcagaaac ccgctgccgg tgagcctgcg ttgttactct
     4981 gggatgatgt aataacctta ttccatgaat ttggtcatac gctgcacggc ctttttgccc
     5041 gccagcgtta tgccacgctt tccggcacca acacgccgcg tgattttgtc gaatttccgt
     5101 cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt cgctcgctac gcccggcatt
     5161 atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa aatgcgtaat gccagcctgt
     5221 tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc acttctcgat atgcgctggc
     5281 attgcctgga agaaaacgaa gcaatgcagg atgtcgatga ttttgaattg cgggcgctgg
     5341 tggcggaaaa tatggatctt cctgctatac cgccacgcta tcgcagcagt tatttcgccc
     5401 atatttttgg tggcggatat gctgcaggtt attacgctta tctgtggacg caaatgttgg
     5461 ccgatgatgg ttaccagtgg tttgttgagc agggcggatt aacgcgtgaa aatgggcagc
     5521 gttttcgcga ggcgatcctt tccagaggta acagcgaaga tctggaacgc ctgcatcgac
     5581 aatggcgtgg taaggctcct cagattatgc cgatgctgca acatcgtggc ttgaacatat
     5641 aaacaccacc aatggctggc ctggagatat cgccaggcca gcctggttat agcagtaatt
     5701 gcagcagctg ggcgagggca aacctcaccg ctaaagctaa taccgtttcg cagtcgccag
     5761 caaaatgcat aaccgcagtg tagttctggc ctttaatatg ccacgcgaac cagacggtac
     5821 ccgctggcgt accatcttca ccgccctccg gtccgccgta gccggtaatg gcaatactga
     5881 catcagcatc cgcacgctct atggcacctg ttgccatttc tgctgccact ttctcactca
     5941 ccgcagaata tcgttcaaga gactgctggc ttacgctgag gattttcatc tttgcctgat
     6001 cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt atcttcagct gcacacaggg
     6061 cgctagccaa cttaccgccg gtgcatgatt cggcggtagt taagcgtaat tgatccgcga
     6121 taagtctttg actcaacgcg gatgtcaggt tttcaatagt gtcggtatct gcgagttgta
     6181 caattttatc tttattaatg ttcac
//
LOCUS       DAJVPT010000171         6053 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVPT010000171 DAJVPT010000000
VERSION     DAJVPT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(40..463)
                     /locus_tag="OQM47_003729"
                     /pseudo
     CDS             complement(40..463)
                     /locus_tag="OQM47_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(649..3957)
                     /gene="icsA"
                     /locus_tag="OQM47_003730"
                     /gene_synonym="virG"
     CDS             complement(649..3957)
                     /gene="icsA"
                     /locus_tag="OQM47_003730"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1481715.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4486..5688
                     /gene="virA"
                     /locus_tag="OQM47_003731"
     CDS             4486..5688
                     /gene="virA"
                     /locus_tag="OQM47_003731"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1481716.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5867..>6053
                     /locus_tag="OQM47_003732"
     CDS             5867..>6053
                     /locus_tag="OQM47_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1481717.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1851 a         1371 c         1034 g         1797 t
ORIGIN      
        1 gtatttttag tcatcctgtt tacctctttc tcaggaagtt tagtctccag gattcccggg
       61 gcggttcagt tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc
      121 aggaccccgt aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt
      181 aggtagacct gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc
      241 tgcacatttc tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca
      301 atattcctcg tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag
      361 agtcatatat tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac
      421 cagtaatctg aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta
      481 atgcaattcc tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg
      541 tttggcattg atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg
      601 tgggtcccag agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata
      661 tttcacaccc aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac
      721 gtttccccag atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat
      781 cacattacgg ttgcctatct ggtgatttga catgccattc ataattacac catattgctt
      841 tgagctccat ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc
      901 gatacaactc tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc
      961 cccagaggat gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg
     1021 cctagttatc tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt
     1081 ccagcgtatg gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga
     1141 atatttctca ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa
     1201 ccaagcatcc atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa
     1261 accggcagta taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt
     1321 tttgctatcc tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt
     1381 gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt
     1441 taattgtcta tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt
     1501 ggtttcgctg agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag
     1561 agaaggacta aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg
     1621 gcgccatgtg tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc
     1681 tcggcgacta ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc
     1741 catctgacta gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag
     1801 gcgatagtca taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga
     1861 agaatctgtc gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt
     1921 tgtcactgca acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt
     1981 actgttgtca ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt
     2041 acctttctga acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc
     2101 gtttttctcc agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt
     2161 aatattattg attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc
     2221 tgaaaaattc aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc
     2281 aactgtcccc atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt
     2341 ttccgccaaa atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt
     2401 tatattatga tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc
     2461 tttcagattg aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat
     2521 caatttattc tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa
     2581 cggattgatg atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag
     2641 cagtttatag tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc
     2701 aaatgacata tttccagaag cttccagact actgattcca gctaaattta aagaactgtt
     2761 ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt
     2821 acaatcacca gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt
     2881 gataattttc agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc
     2941 atggagtatt aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg
     3001 tgttccatca tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct
     3061 accatttata gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat
     3121 tacagacagg ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta
     3181 agcagcacct ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc
     3241 tgtgatggca tcacctccat taccgccatc accctcacca taattgttac ctccattacc
     3301 accggaaata gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc
     3361 attcccccca tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc
     3421 atttattata aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc
     3481 accgctaccg ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc
     3541 ggtaatagag tcaccaccat taccgccaca actattacca tcattattat caccagcacc
     3601 accatgacct gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg
     3661 agaggggttg gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg
     3721 agaaagtcca tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc
     3781 ttgagtaccc gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc
     3841 aacaacaaaa ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc
     3901 ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca
     3961 ctatattatc agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa
     4021 gggacgatat atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt
     4081 atatatcaat gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa
     4141 gctatatgtt cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact
     4201 tttcagttct ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa
     4261 tggttctatc tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc
     4321 acaactttcc taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt
     4381 atcgttttct taaagagaag aataacattc cattttatta tgttcccaat ttaattatat
     4441 taatatatat tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac
     4501 ataactaacc atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa
     4561 gtaagttgga ataaattgag tttttgtgac attttactga aaataataac tttcggcata
     4621 tattctccac acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc
     4681 tcaccatcac tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc
     4741 atttcaatac ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat
     4801 aaaatacaat ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc
     4861 cccaaacaat tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt
     4921 aaaactcaca agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata
     4981 ataccaaacg atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat
     5041 atttacagag atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt
     5101 gaaataccac taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt
     5161 gatagcaaaa aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca
     5221 aacttccccc aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt
     5281 caagaccctg catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg
     5341 tcttcctctg tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt
     5401 aatttcatgc ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca
     5461 gttgtcccta aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc
     5521 tcatcaatat taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca
     5581 agctatctcg ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct
     5641 ccagaaagtc gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata
     5701 ttggctgcca gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa
     5761 gctaaaagga tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc
     5821 cccggaaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     5881 acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga
     5941 atatgactca caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga
     6001 aactctgcgt gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJVPT010000172         6006 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVPT010000172 DAJVPT010000000
VERSION     DAJVPT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..299
                     /locus_tag="OQM47_003733"
     CDS             <1..299
                     /locus_tag="OQM47_003733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1481718.1"
                     /translation="IEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHDLLE
                     VEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(375..839)
                     /locus_tag="OQM47_003734"
     CDS             complement(375..839)
                     /locus_tag="OQM47_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1481719.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(836..1711)
                     /gene="nanK"
                     /locus_tag="OQM47_003735"
     CDS             complement(836..1711)
                     /gene="nanK"
                     /locus_tag="OQM47_003735"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1481720.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1708..2397)
                     /locus_tag="OQM47_003736"
     CDS             complement(1708..2397)
                     /locus_tag="OQM47_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1481721.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2445..3935)
                     /gene="nanT"
                     /locus_tag="OQM47_003737"
     CDS             complement(2445..3935)
                     /gene="nanT"
                     /locus_tag="OQM47_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1481722.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4045..4938)
                     /gene="nanA"
                     /locus_tag="OQM47_003738"
     CDS             complement(4045..4938)
                     /gene="nanA"
                     /locus_tag="OQM47_003738"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1481723.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5060..5851)
                     /gene="nanR"
                     /locus_tag="OQM47_003739"
     CDS             complement(5060..5851)
                     /gene="nanR"
                     /locus_tag="OQM47_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1481724.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1496 a         1648 c         1521 g         1341 t
ORIGIN      
        1 acattgaaca gcttgaatta atccataaaa gtggtgatgt gttgtatcca gtaaaaataa
       61 ccagaaaatc atcaggaaaa actgccttcc accttgttcc attcggctta aataagactc
      121 acgaccttct agaggtggaa gatgcttcag aagcaattcg gctggtgatt gatgaacgtc
      181 attccattcg ctgcagtaca cttacagcaa ctattacgaa taaaaagggc aagcgaatta
      241 agcggacagg tatatacagt atcaagggtg ttaacataaa agaatacaac gtcaggtaat
      301 ggcacagagc ggtccgtcag ttcaggtttg gctctgtgcc aaagatgggt caggtcacac
      361 ctgatctaat aaatttaagc cattaaatca gccttaacct tcaccaccac ctttttaatc
      421 tcacccggct cgccaacaac gcatcccggc ttatgcggct ctcctggcat aaacacggcg
      481 aacattcccg gtttcaggat gatggtttgc tcgttttcaa tggcgctaca aagctggtaa
      541 tcatcttcat ggtggaactc ttcacattgg cgcgcagtgc ctgccatgcc aaacagaatc
      601 cgttcctcac cgtttaataa cagctggata tcaatgtatt gctcgtgcaa ttccgctttt
      661 ttctcgacgg gcgattgagt gttaaacgtc atgacattca taaaaatatt gtcgccctgt
      721 aattcgtaac gacccggcgc tttttcttgc ggtctggcag ctaaggccag cgttaacgcg
      781 tcctgtaacg caggatgtaa cccagcagac ggtaatgact gtacttcacc catcatcata
      841 atttttctcc ctgggccaac agcgcagccc caagtaaacc tgcatcatgg cggtaatgcg
      901 ccgccagtaa atcaacatga aatgccgctg gctcctgcgc cagatacatt tccaccaacg
      961 ccagataccc ttctgccaga ccaacgctgc caccaaccac cacgcactgg caatcagttg
     1021 tggctttaat atcagcgatc agccttgcaa gcgtacgtgc ggagcgatga atcagttgct
     1081 gcgcctgctc atcgccctgc ccggcgcgtg tgaaaatagt tctcgcatcc gcgccagcca
     1141 gctcgccctg cgctgccgcc gcaatgccgc gaccagaagc aattgcttcc acgcaacctg
     1201 tgcgtccaca gccgcagact gggccgtgtg gatcggcaag cgtatgcccg atgtgtcccg
     1261 ccagaccgcc agggccggta agcagtttgc cgccgctcac tacaccgccg ccgacgccag
     1321 tggaaacggt gataaagacc atatcggtta tatcgccttc cagcgcctga tactctgccc
     1381 atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa attggtaagt tgttccagcg
     1441 ttttgactaa cggaaagtgt agcaatccac cgagattatg cggattcagc gccagcaaac
     1501 tgccgtcgcg aataattccg gttgaagcga tggcaacccg ctgcgcatga gcttgcaacg
     1561 gagagactaa tgcggataag gcatcacgca aggcttctgg tgtctggctg gctggcgtag
     1621 gaagttcacg acgatcgcgg atctgcccgt cagcgccaat cagcgcggcg gcaagtttag
     1681 taccgccgat atcaatcgcc agtatggtca tagcaccgcc tttttcatcg ctgtgttgta
     1741 ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc
     1801 gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa cgcccttcgg caatcacccg
     1861 acatccggct tcgctcaacg ttttcaccag cgccagatcc ggctcttctg gcgtttcagg
     1921 cgtggtatag ccagaaagcg tagtgccaat aatttcggct cccagctttt ggcatgccag
     1981 gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa ccgtgatgat ggatacgtgc
     2041 cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg cgataatgtc
     2101 cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag gccgtgatgc gtaccggaga
     2161 atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg cacgcgtggc
     2221 ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct ctgccgctaa
     2281 tgccatggct gcgacgattt cgggtttatc gagcgggctg tcaggaaccg gctggcagga
     2341 gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta acgacataca
     2401 tcttccctta gcgaaaggcc cggtacatag accgggcaac aggattaact tttggttttg
     2461 actaaatcgt ttttggcgct gccaaatggc acggcaccgc taaatggttt gccgtcgata
     2521 gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct gaacgcggga aggcatatcc
     2581 agaccaatca gcaggatcac cacaaatgtc aggctgaacg agagcgatgc cagcgcagta
     2641 cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc gccacccaat
     2701 gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct ggtcggtatc gaaataaccg
     2761 ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg gaagaacagt
     2821 aacagaccga gcacccagac gtttgcgccg ccaatcgcaa ataccggaat aatcagcagc
     2881 tgcgaggcca gcaggctaca aacgtacgct ttgcgggttc ccagccagtc accgaggaag
     2941 ccacctacgc agcatcccac ggccgcgcca aagccactaa agaacagcac attggcgaca
     3001 gtatgcgggt tataagccag atcggttttc agatacgttg gcagcagcgc ctgaatcggc
     3061 catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgcccgt tggccagcgt
     3121 ttacccgtac tttgcaccat aaagctgata aagattgcgg cgcataacag cccgagaaca
     3181 gcgacgattg cagcattttg caggttaccg gcgaagcaga accacagcgc agtagccgcc
     3241 gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat atccaccatt
     3301 gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttccgcttc cgggatgttt
     3361 ttacgcagcc agagagcaaa gatgattggc aaaatgccga taaagaacag cgcacgccaa
     3421 ccccagaccg gaaccaccag gctatagacc tgagcggcaa cgacggcccc cacagagaag
     3481 cctgaaatca aaaaaccact ggctttgtta cgcagatgtt ttggccagct ttcaatgaca
     3541 taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag acgagcgata
     3601 aacatggtga tgtagcctgg cgcaaagccg caggccagcg tcccggccga gaagagaacg
     3661 atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc gagcatcagg
     3721 ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc
     3781 agcccgaatt caccctgtac ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg
     3841 tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc attgtgcacg gttgagatgg
     3901 cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg
     3961 cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc ctcacccggt
     4021 aggggcgagc gaggggaaaa caactcaccc gcgctcttgc atcaactgct gggccagcgc
     4081 cttcagttct ggcagatatt tttcatctac cggtccaaac ggtttgcggc acagcggcac
     4141 agaaacgaca tccatataat gcaggacagt tttcaggccg cggaatacgc ccgttttgat
     4201 cagtaaatca atgactttgt tgcactcagt ttgcagtttc tgcgcggtct ggatatcgcc
     4261 ttctttcagc gccttcacga tcccctgata tcgccagccc atgatgttgt aagtactgcc
     4321 gataccacca tcagcgcctg ccagcaagcc ggaagcgaag atttcgtcgt aaccgttata
     4381 gagcaccaga tcaggatgtt cacgacggat ctgttccatc tgatagagat cgccagaagt
     4441 ctgtttcagc gcaccgacgc caggcaacgt aaccagggtg ttgatctgat ccagggtcag
     4501 ttttacccca ctcagggctg gaatgttgta caccaccatc ggcaaaccat ccgctgaatc
     4561 aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt
     4621 gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt gttggctttc
     4681 ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg cctcttcggc
     4741 gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggctt cgccggtcga
     4801 accacccacg tataaaccgt cgatgccctg ctgaatattg aactgaacca ggcggcgcag
     4861 actcgcttta tccagtgctt gttgttgatc aaaaggagtc aggagtgcag ccattacgcc
     4921 acgtaaattc gttgccataa atacctctga agtgatgctt gtctgataaa cgatatacct
     4981 ttatacctgt tataccagat caattaagca acaccccata cagaaagctt ataatgcgat
     5041 ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaaa gcgtgccagg
     5101 tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca ggatcatgac
     5161 ggcggatcgc atcaacgatc gcaatatgct gttgataact aacgttgtta tgttcgtgca
     5221 gtgcctgatc ggtaaccgtt gggcgtgcgg caataagcca gtcgagcagg gcaacgtgga
     5281 tcgccatgaa gattgggtta ccggggatct ccgccagcac gcggtggaaa tcaacgtctg
     5341 aacgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt gcttttgcca
     5401 gcaaatcgat ttgctcatcg gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa
     5461 agaacagacg taattgttcg aaatgggcaa tcccaccagg atgagaaagg aaatctttcg
     5521 ccatgccgga aagctcgccg atgatagtgt ccgcagaagg tcgagagaca cgagcgcgtt
     5581 cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct tcacgcacag
     5641 aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat tgttcacctt
     5701 cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt tcggagagtt
     5761 ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg
     5821 tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat taacatcatc
     5881 agtgacatcc tatcacagga ttgaaagtag gggaaaatgg caggtttttc tctttgtgcc
     5941 tcatcattac cataattaac ggaataatta actattgcga aaaattaatg taacgcagat
     6001 aaacac
//
LOCUS       DAJVPT010000173         5913 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVPT010000173 DAJVPT010000000
VERSION     DAJVPT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            11..490
                     /gene="mddA"
                     /locus_tag="OQM47_003740"
     CDS             11..490
                     /gene="mddA"
                     /locus_tag="OQM47_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1481725.1"
                     /translation="MAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTIAGYPVLVSE
                     EDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHV
                     MVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQLQLDERTEPDAIG"
     gene            487..936
                     /locus_tag="OQM47_003741"
     CDS             487..936
                     /locus_tag="OQM47_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1481726.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(937..1170)
                     /locus_tag="OQM47_003742"
     CDS             complement(937..1170)
                     /locus_tag="OQM47_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1481727.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1256..1429)
                     /gene="ortT"
                     /locus_tag="OQM47_003743"
     CDS             complement(1256..1429)
                     /gene="ortT"
                     /locus_tag="OQM47_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1481728.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1624..1719
                     /gene="yncL"
                     /locus_tag="OQM47_003744"
     CDS             1624..1719
                     /gene="yncL"
                     /locus_tag="OQM47_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1481729.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2121..2930)
                     /locus_tag="OQM47_003745"
     CDS             complement(2121..2930)
                     /locus_tag="OQM47_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCS1481730.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3140..4564)
                     /gene="patD"
                     /locus_tag="OQM47_003746"
     CDS             complement(3140..4564)
                     /gene="patD"
                     /locus_tag="OQM47_003746"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS1481731.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4586..5380)
                     /locus_tag="OQM47_003747"
     CDS             complement(4586..5380)
                     /locus_tag="OQM47_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1481732.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5370..5717)
                     /locus_tag="OQM47_003748"
                     /pseudo
     CDS             complement(5370..5717)
                     /locus_tag="OQM47_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1543 a         1505 c         1324 g         1541 t
ORIGIN      
        1 ctgtgctgcc attgcggaaa tttataacca cgccgtgttg tatacggcgg ctatctggaa
       61 tgaccaaacg gtggatgcag ataaccgcat tgcctggttt gaagcgcgga ccatagcagg
      121 ttatccggtg ctggtaagtg aagaagatgg cgtagttacg ggatatgcct cgtttggcga
      181 ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat tcggtttatg tccatcccga
      241 tcatcagggc aaaggtctgg ggcgtaaatt gttaagtcga ttgattgatg aagcgcggga
      301 ttgcgggaag catgtcatgg tcgccgggat cgaatcgcaa aatcaggctt cgctgcatct
      361 gcaccagtcg ctgggatttg tcgtcaccgc acaaatgccg caggtaggca ctaaatttgg
      421 tcattggctg gatctgacat ttatgcagtt gcaactcgac gagcgcactg aaccggacgc
      481 gattggatga atcagtcgct cacccttgct tttttgattg ccgccggaat tggtctggta
      541 gtccagaaca cgctgatggc gcgcatcacc cagacctcct ctaccattct catcgccatg
      601 ttgctgaact cactggtggg gattgtgctc tttgtttcta ttttatggtt taaacaaggc
      661 atggcggggt ttggcgaact ggtgtccagc gtgcgctggt ggacacttat acctggcttg
      721 ctgggttcgt tttttgtctt cgccagtatc agcggatatc agaatgtggg agcggcgaca
      781 actattgcgg tactggtcgc cagccagctg attggcggtc tggtgctgga tatcttccgc
      841 agtcatggcg taccactacg ggcgttgttc ggcccaatat gtggcgcaat tttacttgtg
      901 gttggcgcct ggttggtggc cagacgctca ttttaatcag aggatggtgc cgcctttggt
      961 taactgttcg tggcgcgctt ccatatcttc tttatgcttg cgaccgtgat gagcaatagc
     1021 ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga cttaactccg cgtcatcgct
     1081 cagggcgatt aataattcgt tcatatgggc aatgacgctc tcttcaatcg cggcatccac
     1141 gcgcgcgatg acttcatcca gatgcgacat actttctcct tagccacttg tgaaatttat
     1201 cgattaatcc aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa
     1261 acagtgaaaa caacagcgcc accgggaaac tcatcggcca tgtggccccc accagaaagg
     1321 cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga
     1381 ttgctgccat aaccgcataa aaaacaagca tatgttgata gagaaacatg tacgggcgtc
     1441 cagccatatg aataaaacgc gcgcaaatat gcgccatttt ttgacgtgca tcaagttttt
     1501 tcaccagttt gagaaaaaat catcaaacag caacttgccc ttatccatct tcagaatgag
     1561 tattattgcg gattttctta acccgtacta tacacatagg ggcaatgata aaaggtggca
     1621 aaaatgaatg tttccagtag aactgtagta ctgataaatg tctttgctgc tgttggtttg
     1681 tttactctta tctctatgag atttggctgg tttatttgat gtagtgattt tgcacgcagc
     1741 acgtactgtc agagaaatca actctctgac agtacgcaag atcttctcat actctccaag
     1801 tttgcaacat tatccttaat atttacttct ttttgctggc atctacattg aaatgagtag
     1861 aaaacatccc cctactgtcg tctatacaaa cagcttactg attcaaacgg aatcttgttt
     1921 tcacattttt ctatcacgaa tactttatac gcgcgggagt ctataaatgc tttatacttt
     1981 caaggaataa cgtaaatata aaaagatggc gtaaagtata tgcatggcag gatgtttgca
     2041 acctccttga tgacgctgcc ccattgaaat tgcaaacccc gccctggtta tcttgcccat
     2101 ttatcatgca aaacagataa ttataggcct ctgaatacct cagaaagatt gaatttttta
     2161 tagtccctct cttgaatatt tccatttgat atcaatcgat tcatctcttc aacaaaataa
     2221 tttatatcat cacacttatt attatccgga agatctgagt agttataatt ccaccatttt
     2281 attctcaaca gttcatcaat aacatttgaa tcaaacctaa atctgattat ttttgcagga
     2341 actcctgcaa ctatggcata tgggggcaca tctttggtaa caacggaatt ggcagcaata
     2401 acagcaccat cacctattgc gatgccacct ttcaatacaa catcatttcc tatccagaca
     2461 tcatttccta taatcggatt ttcaggtttg gtaatgaatg gtttcaatgt ccagctacgt
     2521 tcaaattcac tcatagcaaa cttatcaaat tcaccgttat aagtcatcat gtgagtagta
     2581 aaacgattca aaggatgatt tccacccatt atttttacat ttgatgcaat actacaaaat
     2641 cgacctatac gtatattccc tgaaaaatgg caggtcggaa atgaaaaagc gccgacactg
     2701 gagaaatttt tcacaggcat tgttgaatat tcttcagcaa taacattatc ttcaattgtt
     2761 atcttgtcac cttttgagaa aagaaataaa ttcctttctt tatacgactt cttcccctta
     2821 tgatttaaaa agattttatt atccatacaa aactcaaagt gttttttcgt ccatctgaag
     2881 gtaagggttt tatttttatc ttccgtgaat ttgtacaatt gaactttcat tgttaatact
     2941 tctacttata aagtattagt ttaataacga tactctaaag ccttatgcta atcaactgtg
     3001 acaggatgaa aaatcgccat caaacattta gcctaatttt tacgccagtt attaatgtaa
     3061 tcattctaat acttaaccgc cattaaactc agaacctaaa acgatagtgg ccgtcctata
     3121 acagccacta acatctaact taatgtttaa ccatgacgtg gcggacgacg gtgtaatcct
     3181 ccagcccata aagtgacata tccttgccgt aaccagaaag tttctgccca ccgtgcggca
     3241 tttcacttac cagcatgaaa tgggtattga cccaggtaca accatattgc agccgtgcgc
     3301 tgacgcgatg cgccctgccc acatctttcg tccataccga agatgcaagt ccgtactggc
     3361 tgtcattcgc ccagttcacc acctgttctt cgttgtcgaa gggcgtaaca ctcactactg
     3421 gaccaaatac ctctttttgc acgatggcat cgtcctgtaa tgcgccagcc agcagcgtcg
     3481 gcgcatagta atagccatta cccttgcgct tttcaccgcc agtgatcact ttgatgtgcc
     3541 ctgtcgcttt cgcctcttct actgccttgc tgacgcgttc gagatgcgcc agcgagctta
     3601 aaggtccaag ctccgtagac tcgtcatccg gcgcaccaga ttttaacgtt gccaccgcag
     3661 cacccagttt ttccaccagc gtatcgtaaa tgcctttttg cgcgtagatc cgacaagccg
     3721 cagtacaatc ctgtccagca ttgtaatagc caaatgtacg tacaccttcg accactgctt
     3781 caatatccgc atcatcaaaa acaatcactg gcgctttgcc accaagttcc atatgagtac
     3841 gcttaatgga cgacgcggta tggctgatga tgtgctcgcc ggtggcgata gagcccgtca
     3901 gcgacaccat ccgcactttg ggatgaccgg tcagcggatc acccaccgtt ttgcctctgc
     3961 caaacagtat gttaatcacg cctgccggga agatatcttt cgccagctct gccaacttca
     4021 acgcggtcag cggggtaatt tctgatggtt taagcactac gcagttccct gccgccagcg
     4081 ccggagcaag tttccacgcg gccatcatca gcggataatt ccacggtgcg atagaagcca
     4141 cgacccccaa cggatcgcga cggatcatcg aagtatgacc ttcaagatat tcacctgccg
     4201 ccagaccatt cagacagcgc gccgcacccg cgaaaaagcg aaaaacatcg acaatcgccg
     4261 ggatttcatc attgaacgca ctatgcagcg gtttgccaca attacgggat tccagttcgg
     4321 caaaaacctg accattttct tcgataacat ccgccagttt cagcaggcat tccgcacgcg
     4381 ctttcggtgt ggtttgcccc cattcagcaa atgctgcatc tgccgcacgc acagcagcat
     4441 cgacctgctc tgcggatacc tcggcaatct ccagtaaaac gtcccccgtt gccggattat
     4501 agacaggctg tttttcccct tcgccgctaa cccgttctcc gttaatcagt aacttatgtt
     4561 gcatagctta ttcctgtatc agtggttatt gaccattgtc gccttcgcgg gttagccacc
     4621 aggcccccag gatcggcaag gttgttacca acataaccag cagtgccacc acgttggtta
     4681 ccggtacatc acgcggtcgc ccaagctgat tgagcaacca caacggtaac gttcgttcat
     4741 gacctgccgt aaaggtcgta acgatgattt catcgaacga caaggcaaac gccagcattc
     4801 ctcctgccag taacgccgaa ctgagattcg gcaacactac gtagcggaag gtttgccagc
     4861 cattggcccc aagatccatt gacgcctcaa ccagactcca cgaggtgcgg cgaaaacggg
     4921 cgatgacatt gttaaacacc accactacgc aaaaagtcgc atgaccgacc acgatggtga
     4981 aaaatcccgg ctccagattg atggttttaa aggcggttaa tagcgccaga ccagtgacaa
     5041 tgcccggcag cgcaatgggc agcagcaata acagcgaaat ggcgtttttg ccgaaaaagt
     5101 ctcgtctcca cagcgcggca gctgccagcg tccctaacac cagcgcaatt aatgtcgcca
     5161 gcgccgccac tttaagtgac agcgtcacgg catcaagaat atcactacgc tgtgctgcca
     5221 cgctaaacca gcgcagcgtc aggccctgcg gtggaaaact aaacgccgca tcttcagtgt
     5281 taaacgcata ggcggcgatt atcaggatgg gaaaatgtag gaaaacaacg ccgccccagg
     5341 ccgccagttt gaggaaaaac ggtgcgcgtt cagagtgcat cgaacgctcc cagacgtttc
     5401 acgaacgcca ggtacagtgc gatgagaata atcggcacca gggtgaatgc cgccgccatc
     5461 ggcatattgc caatcgcccc ctgctgggaa taaaccatat tgccgataaa atatcctgga
     5521 ggaccaacca gctgcgggac gataaaatcg cccagtgtga gtgagaaggt aaagatagag
     5581 ccagcggcaa tacccgggat tgccagcggc agcaccacat agcgaaaggt ttgtcgtgga
     5641 cgtgcgccga gatcagccga cgcctgcaac aatgacggcg gcaaacgctc aagcgccgcc
     5701 tgaacgggca ggatcatcga gtaggcagcc tgacggctgc ggcttgtcat ggtctgcaat
     5761 taccgttata aaaacaggca atgtcattgt ctttcaggtg gttatatggc ccgttcagct
     5821 aaaccccgta aacgtaaacc cgcctctcaa agttcctgag tcgtgacccg ttcgaatgcg
     5881 tgctgtgtgg cggccggatg gtataccgcc ggg
//
LOCUS       DAJVPT010000174         5876 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVPT010000174 DAJVPT010000000
VERSION     DAJVPT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1230
                     /locus_tag="OQM47_003749"
     CDS             <1..1230
                     /locus_tag="OQM47_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1481733.1"
                     /translation="RPLGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHH
                     AIRTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTI
                     KAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNS
                     IQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRL
                     NMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYS
                     ESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLM
                     RWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETR
                     NTYTSRFTEVAMAFASI"
     gene            1320..1793
                     /locus_tag="OQM47_003750"
                     /pseudo
     CDS             1320..1793
                     /locus_tag="OQM47_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1958..>5876
                     /gene="pic"
                     /locus_tag="OQM47_003751"
     CDS             1958..>5876
                     /gene="pic"
                     /locus_tag="OQM47_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCS1481734.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLL"
BASE COUNT         1564 a         1370 c         1592 g         1350 t
ORIGIN      
        1 cgaccactgg gtatccccgc gttgcgggat cgtattgttc agcgggccat gctgatggcg
       61 atggagccga tatgggagag tgattttcat acgctctcat atggcttccg gcctgagcgc
      121 agtgtccacc acgcgatccg cacggtgaaa ttacagctca cagactgtgg tgaaacccgg
      181 ggacgctggg tgattgaagg cgacctgtcc agttacttcg acaccgtaca tcatcgactg
      241 ctgatgaagg ctgtacgccg caggatcagt gacgcacgtt tcatgactct gctgtggaaa
      301 accatcaagg cgggacatat cgatgtcggt ctctttcggg cggccagtga aggtgtgcca
      361 cagggcggtg ttatatcgcc gctattatcg aacatcatgc tgaatgagtt cgatcaatac
      421 ctgcatgagc gctacctgag cgggaaagcc agaaaagatc ggtggtactg gaataacagt
      481 atccaacggg gccgaagtac ggcggtcaga gaaaactggc agtggaaacc cgcggtggcg
      541 tactgccgct atgccgatga ttttgtcctc atcgtcaaag gcaccaaagc acaggcggaa
      601 gccatcaggg aggagtgtcg gggtgtgctc gaaggcagtc tgaaactcag gctgaacatg
      661 gataagacta aaatcaccca tgttaatgac ggctttatct ttctggggca caggatcatt
      721 cgcaaacgca gtcgttatgg cgagatgcga gtggtctcaa cgatcccgca ggagaaagcc
      781 agaaacttcg ccgcatcgct gacagcactg ttatcaggca actacagtga aagcaaagtc
      841 gatatggctg aacaactcaa ccgaaaactg aaaggctggg ccatgttcta tcagttcgtt
      901 gattttaagg ccaaagtctt cagttatatc gaccgtgtcg tgttctggaa gctggctcac
      961 tggctggccc gcaaataccg tacaggtatc gcttccctga tgaggtggtg gtgtaaatca
     1021 ccgaaaccgg gtcagagcaa aacgtgggtt ttatttggta aaaccaatca cggcaagctc
     1081 agcggcgaaa tactgtaccg gttggtgggg caaggcaaga agctgttccg ctggcggcta
     1141 cccgaaggta atccctatct gaggacggag accagaaaca cgtatacatc gcgctttaca
     1201 gaagtggcaa tggcgttcgc cagcatttaa atggagagcc ggatgcgctg aaaggtgcac
     1261 gtccggttcg gggaggagag gcagggaaat agtccgacta cgccctgcct cttactctac
     1321 tcagcacatg gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt gccggataca
     1381 tcgtgtggtg acgagtctca tcgtctatgg aaacgacatt cgtgctggat gcactggagc
     1441 aggcgttgtg ggcccgtcgt ccgtctggca ccatccatca cagcgataaa ggctctcagt
     1501 atgtgtcact ggcctataag gcgcgactaa aagaagcaaa actgctggca tcaacaggga
     1561 gtactggtga ctcgtatgac aacgcgatgg cagagatcat caagggcctt tacaaagcgg
     1621 aggtaataca ccgtaagagc tggaaaaacc ggacagaagt ggagctggcc acactcacgt
     1681 gggtggactg gtataacaat cgacgattgc tggaaaggct gggccatatc ccaccggcag
     1741 aagcagaaaa agcttattat gcttccatcg gaaatgatga tctggcagcc tgagttcaca
     1801 gataaaacac tctccaggaa acccggggcg gttcagttca caaaaacaca ttaatgcagt
     1861 aactatattt tcctttctgg tgataacgtc gggttatcat tagcttcttc agctatttta
     1921 cttttatatc ccttgtaaac atcatggaga atccatagtg aataaagttt attctcttaa
     1981 atattgcccc gtcaccgggg ggcttattgc tgtctctgaa cttgcccgca gggtaataaa
     2041 aaagacatgc cgaagattaa cgcatattct tctggctggc attccagcaa tctgtctgtg
     2101 ttactctcag atatcccagg cgggtattgt ccgttccgat attgcctatc agatttatcg
     2161 tgatttcgcc gaaaacaaag ggctttttgt acctggtgcc aatgatattc cggtatatga
     2221 taaggacgga aaacttgtgg gaagactggg taaagcccca atggccgatt tcagcagtgt
     2281 gagctcaaat ggcgttgcta cgcttgtatc gcctcagtat atcgtcagcg taaagcataa
     2341 cggaggatat cggagtgtga gctttggtaa tgggaaaaat acatattccc ttgttgaccg
     2401 taataaccac ccttctattg acttccatgc tccacgtctg aataaactgg ttacagaagt
     2461 tattccctca gcggtaacat cagaaggaac caaagccaat gcttataaat acactgaacg
     2521 ttacaccgct ttttatcggg tgggtagtgg tacgcagtac actaaggaca aggacggaaa
     2581 tttagttaag gttgccggtg gatatgcttt taaaacagga ggaaccacag gagttcctct
     2641 gatatctgat gcaacaatag tctctaatcc cgggcaaact tataatcctg taaacggccc
     2701 tttacctgac tatggagccc ctggggatag tggttctcct ttgtttgctt atgataaaca
     2761 acaaaaaaaa tgggttattg ttgctgtatt aagagcatat gcaggtatta atggtgctac
     2821 gaactggtgg aatgtcatac caacagatta tctgaaccag gttatgcagg acgatttcga
     2881 tgcccccgta gactttgttt ccggactggg ccccctgaac tggacatacg acaaaacatc
     2941 aggcacaggt accctgagcc agggcagtaa aaactggacc atgcacgggc agaaagacaa
     3001 tgacctcaat gccggtaaaa atctggtatt cagcgggcag aatggtgcaa ttatcctgaa
     3061 agacagtgtg actcagggtg ccggttatct cgaatttaaa gacagttaca ccgtatctgc
     3121 tgaatccgga aaaacatgga cgggtgccgg cattattact gacaagggga cgaatgtaac
     3181 ctggaaggtc aacggcgttg ccggtgacaa cttgcataag ctgggggaag gaaccctgac
     3241 cataaacgga acaggtgtaa acccgggagg actgaaaacg ggagacggta tcgttgtact
     3301 taaccagcag gcagacactg caggtaatat ccaggccttc agttcagtga acctcgccag
     3361 cggacgtccg accgtggtgc tcggggatgc ccgtcaggtc aatccggata acatttcatg
     3421 gggataccgg ggaggtaagc ttgaccttaa tggtaatgcc gttaccttca cccgactgca
     3481 ggctgctgat tacggggcgg tgattacaaa taatgcacag caaaaatccc agcttttact
     3541 ggatcttaag gctcaggata caaatgtcag tgaaccgacg attggaaata tatccccctt
     3601 tggtggtacc ggcactccag gaaacctgta cagcatgata ctcaacagcc agacccgctt
     3661 ctatattctg aaatctgcca gctatggtaa cactctgtgg gggaacagcc tgaatgatcc
     3721 ggctcagtgg gagtttgttg gcatgaacaa aaacaaagca gttcagacag taaaagatag
     3781 gatcctggcc gggcgggcaa aacaacccgt tatctttcat ggtcagctga ccgggaatat
     3841 ggatgtcgcc attccacagg tgccgggggg aagaaaggta atctttgatg gtagcgtgaa
     3901 cctgccggaa ggtaccctga gtcaggacag tggcaccctg atattccagg gacatccggt
     3961 tatccatgcc tccatcagtg gcagtgcacc ggtcagcctg aaccagaaag actgggaaaa
     4021 ccgtcagttt acaatgaaaa cactgtcgct gaaagacgct gacttccatc tttcacgtaa
     4081 cgcctcgctg aacagtgaca ttaagtcgga taacagccat atcacactgg gaagtgacag
     4141 ggcatttgtg gataaaaatg acggaacagg aaattatgtc attccggagg aaggtacctc
     4201 tgtcccggac accgtgaatg acaggagcca gtatgaaggg aatattacgc tgaaccataa
     4261 ctcagccctg gatatcggca gcaggttcac cggggggatt gacgcttatg acagtgccgt
     4321 cagcatcacc tctccggacg tcctgttgac agccccgggt gcttttgccg gcagttcact
     4381 gacagtgcat gatggcggtc atcttacagc actgaacggt cttttcagcg acgggcatat
     4441 tcaggccggt aagaacggca aaatcaccct gagcggtaca ccggttaaag atacggctaa
     4501 tcagtatgcc cctgctgtat atctgacgga cggatatgac ctgaccggcg ataacgcagc
     4561 actggaaatt acccgtggag cacatgcttc cggtgatatt catgcctctg cggcatcaac
     4621 agttaccatc gggtctgaca cgccggcaga actggcttct gcggaaacgg ctgcatcggc
     4681 gtttgccggc agtcttcttg agggctataa cgcagcattc aatggtgcca taaccggtgg
     4741 cagggctgat gtcagtatgc ataatgcact gtggactctg ggtggggact ctgccatcca
     4801 cagtcttacc gtcagaaaca gccgtattag ttctgaagga gaccgtacat tccgtaccct
     4861 gacggtgaat aaactggatg caacaggcag tgattttgtt ttgcgtacgg acctgaaaaa
     4921 tgccgataaa attaatgtga ctgaaaaagc cactggttca gataacagcc tgaacgtcag
     4981 ctttatgaat aatcctgctc agggacaggc cctgaatatt cctctggtca cggcaccggc
     5041 gggaacttca gcagagatgt ttaaggccgg cacccgggtg acaggtttca gtcgggtgac
     5101 cccaaccctg catgttgata ccagtggtgg caatacgaag tggatactgg atggttttaa
     5161 agcggaggct gataaagccg ctgccgcgaa ggctgacagt ttcatgaatg ccgggtataa
     5221 aaacttcatg acggaagtta acaatctgaa caaacgtatg ggtgacctgc gtgacacaaa
     5281 cggtgatgcc ggtgcctggg cgcgcatcat gagtggtgcc ggttctgcag acggtggtta
     5341 cagtgataat tacacccatg ttcaggtcgg ctttgacaaa aaacatgaac tggacggtgt
     5401 ggacctgttt accggtgtca cgatgaccta taccgacagc agtgcagaca gccatgcatt
     5461 cagcggaaag acgaaatcgg tggggggcgg tctgtatgct tcagcattgt ttgagtccgg
     5521 tgcctatatc gatttgattg gtaaatatat tcaccatgac aatgattaca caggtaactt
     5581 tgctagcctg ggaacgaaac actacaacac ccattcctgg tatgccggtg ctgaaacggg
     5641 ttaccgctat cacctgacag aggacacgtt cattgagccg caggctgaac tggtttacgg
     5701 cgccgtgtcc gggaaaacat tccgctggaa agacggtgat atggacctga gcatgaagaa
     5761 cagggacttc agtccgctgg ttggaagaac aggggttgaa ctgggcaaga ccttcagtgg
     5821 taaggactgg agtgtgacgg cccgtgccgg aaccagctgg cagtttgacc tgctga
//
LOCUS       DAJVPT010000175         5868 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVPT010000175 DAJVPT010000000
VERSION     DAJVPT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            420..1328
                     /locus_tag="OQM47_003752"
     CDS             420..1328
                     /locus_tag="OQM47_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1481735.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OQM47_003753"
     CDS             complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OQM47_003753"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1481736.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3643..4401
                     /gene="loiP"
                     /locus_tag="OQM47_003754"
                     /gene_synonym="yggG"
     CDS             3643..4401
                     /gene="loiP"
                     /locus_tag="OQM47_003754"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1481737.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OQM47_003755"
     CDS             complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OQM47_003755"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1481738.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5663..>5868)
                     /locus_tag="OQM47_003756"
     CDS             complement(5663..>5868)
                     /locus_tag="OQM47_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481739.1"
                     /translation="HTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1442 a         1532 c         1527 g         1367 t
ORIGIN      
        1 aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc cttattttat
       61 ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg aaagcaattg
      121 ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata acaacaatat
      181 gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa atcaacattt
      241 tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt cgaaaacata
      301 cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt ctcacctgtc
      361 ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg aagattgtca
      421 tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct atcgccattg
      481 gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat gaaggtgaac
      541 gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg ctgggtttta
      601 agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct gtggtctatc
      661 agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta tcagtcagct
      721 tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact attgaagcat
      781 taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt ttcggccaat
      841 ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg caaaatgcca
      901 tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa aatattgatt
      961 tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc gccattgcca
     1021 cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta actcaggccc
     1081 aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact attcgcgtaa
     1141 gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca gaagcaatcc
     1201 gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc actgccgaac
     1261 gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg tttatctcta
     1321 ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac tgattacagc
     1381 agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc aaacagcagc
     1441 tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt caggccaaca
     1501 tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc
     1561 ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg
     1621 gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc aaccgccagt
     1681 tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca gtctttcagc
     1741 acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg ctgcgccagg
     1801 ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac
     1861 gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc
     1921 agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag accgatggag
     1981 tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg tacggcgtta
     2041 cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg
     2101 gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg gatgtagtta
     2161 cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt agacggtgcc
     2221 aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc gatagcattc
     2281 tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc gataaactct
     2341 ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt aaattcagcc
     2401 gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc agattctttc
     2461 gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat ttcgaacggt
     2521 gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca
     2581 ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat ggttttgcac
     2641 atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg tttgatggat
     2701 gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt
     2761 gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc gttgtcatcg
     2821 tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac ttcgtgggag
     2881 atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg
     2941 tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat accgactgcg
     3001 ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt ataacctact
     3061 tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc ttccatcggc
     3121 agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg gccgttggac
     3181 agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt caggaaatca
     3241 cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg accggatttg
     3301 gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc tttacgtgag
     3361 gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg acgacagcgc
     3421 gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg tcatgtacat
     3481 ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc
     3541 tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac ctgcaaacgg
     3601 cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta
     3661 ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga ctccaacgga
     3721 ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa
     3781 accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc gccagccaat
     3841 agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa tatcaacggt
     3901 cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc aatggctaac
     3961 ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga agtcgaagcg
     4021 gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg
     4081 gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat tgtcggcagt
     4141 ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca attctcccag
     4201 cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg cggcatcagc
     4261 ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg tcgtcaaagc
     4321 tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc
     4381 agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat aaggcgttca
     4441 cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg atgcgatgct
     4501 ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt tcacgccgca
     4561 tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt
     4621 tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga gcggtgattt
     4681 ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg ttgagatctt
     4741 tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc actggcgtac
     4801 cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg taaaccggca
     4861 tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct
     4921 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga ataccaatct
     4981 gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac atagtgccgt
     5041 ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt accagcgcca
     5101 ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
     5161 cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt
     5221 cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc acgacgttca
     5281 gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg
     5341 aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa gtggccatat
     5401 cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg aagttcatcg
     5461 gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga tgacctaagg
     5521 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg
     5581 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg
     5641 ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag ccattgttat
     5701 ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt
     5761 caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc gaattggctg
     5821 cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgtt
//
LOCUS       DAJVPT010000176         5845 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVPT010000176 DAJVPT010000000
VERSION     DAJVPT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..87)
                     /locus_tag="OQM47_003757"
     CDS             complement(<1..87)
                     /locus_tag="OQM47_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCS1481740.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVR"
     gene            323..595
                     /locus_tag="OQM47_003758"
     CDS             323..595
                     /locus_tag="OQM47_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1481741.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(561..968)
                     /gene="nudG"
                     /locus_tag="OQM47_003759"
     CDS             complement(561..968)
                     /gene="nudG"
                     /locus_tag="OQM47_003759"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1481742.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1055..1675
                     /locus_tag="OQM47_003760"
     CDS             1055..1675
                     /locus_tag="OQM47_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1481743.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1684..2991)
                     /gene="ynjE"
                     /locus_tag="OQM47_003761"
                     /pseudo
     CDS             complement(1684..2991)
                     /gene="ynjE"
                     /locus_tag="OQM47_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3058..3711)
                     /locus_tag="OQM47_003762"
     CDS             complement(3058..3711)
                     /locus_tag="OQM47_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1481744.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3711..5182)
                     /locus_tag="OQM47_003763"
                     /pseudo
     CDS             complement(3711..5182)
                     /locus_tag="OQM47_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5155..5845)
                     /locus_tag="OQM47_003764"
                     /pseudo
     CDS             complement(5155..5845)
                     /locus_tag="OQM47_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1384 a         1564 c         1541 g         1356 t
ORIGIN      
        1 acgaacggct tgcgcgaact cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa
       61 tgactccaga gaatatgtct gatccataga tataaaaacc ttatatatta atacgattgt
      121 gcttatgttg tttatgtcat gtgcttttgc agttttcgac tataccatca agaatgtgac
      181 ggtaagcaag aaaaaaatga cccaggaaag caaattaata acgagagtaa tctcataacc
      241 aaatgcgata actcagacgc taaagttttg ccacccggct accgttatca gtagcagaga
      301 caataacgtt aaggagttcc ctgtgagtcg agcattgttc gccgtagtat tagcatttcc
      361 attaattgca ctggctaacc cgcattatcg tccggacgtg gaggtaaacg taccgccaga
      421 agtgttcagt tcaggcgggc agtcagcgca gccctgtact cagtgctgcg tttatcagga
      481 tcaaaattat tctgaagggg cggtaattaa agccgaagga atactgctgc aatgccagcg
      541 tgatgacaaa acccttagca ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc
      601 tctaataatg gaatgtcagc aggggccagc ggatattgca gcgcctcttc aggtgagcac
      661 cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag
      721 gcatgaagat ggataatccg gcccgaaact tctcgctgat ggctggcaac atactcaccc
      781 acagttgctt cgatgtccaa ttcttcgttt aactcacgca ccagcgcctg ctgctggctt
      841 tcatccagct cgactttacc tccggcaaac tcccataatc ccgcctgatc gctctgggcg
      901 gggcgttgcg cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc
      961 attttcataa attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc
     1021 tcaatcagaa aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg
     1081 gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga
     1141 tggtttaaca ttagtgggat ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg
     1201 ctggtatctg gcggcgctag tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg
     1261 tgcgctggcg cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga
     1321 ttttctcttt tacgcgctgg tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc
     1381 gctggcaggc ggctggttgt tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt
     1441 tgctgcactg gcagcaaaac atcagattga taaccccggt tatgcacata agtcgtttta
     1501 ctatctgggg ggattaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt
     1561 attccctgca tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac
     1621 ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca
     1681 atgttatttg ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct
     1741 gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc
     1801 gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc
     1861 tggtttgata ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc
     1921 atccgggtta tagaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc
     1981 tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc
     2041 tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg
     2101 ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc
     2161 tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca
     2221 gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg
     2281 cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg
     2341 ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc
     2401 cacttcgttg gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg
     2461 agcgccctag gccgcttcaa tgactttcca gtcaccgaca ggtttcgccg taacctcttt
     2521 accttgttgc aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt
     2581 ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc
     2641 tttttgcagt cgcgttttga cggcgtcaac atctttgcca ttaccgtaca gcgccaccgg
     2701 ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt
     2761 gtccagccag ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa
     2821 ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg
     2881 ttgctgaagt tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca
     2941 agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc
     3001 cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta
     3061 tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg
     3121 aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct
     3181 gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc
     3241 caggagtaac gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg
     3301 accgccagac aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag
     3361 tgcatcattc acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag
     3421 taaattttgc ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat
     3481 ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt
     3541 acaagaaaac tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca
     3601 gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt
     3661 cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt
     3721 ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc
     3781 ggtaacaaca gttgccataa cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt
     3841 aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc
     3901 atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac
     3961 atgagtgggc atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca
     4021 atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac
     4081 ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc
     4141 catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac
     4201 catagccaca actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa
     4261 gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc
     4321 gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa
     4381 aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg
     4441 cggggaatag tgcgccgcca gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag
     4501 cagactggcg agtgcgcctt tcgtttgttg atccgcgtcg ccctgagtta accactgcca
     4561 gctaattact gccagcgtcg gtggattacc tggcccaaga ataatcgcca catccacgac
     4621 cgaaagcgac caggcaacaa tcgccagcat cgccattgcc agcggctgta gcccagcgaa
     4681 tccagcacaa tgacctgctg caacagccgt ttttcgctca atactgccac taagatccac
     4741 agcagaaatg cgctttcttt cactgcaagg gtgaggccca gaccgatcca aatcggtcca
     4801 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     4861 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     4921 tacgctgcca tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg
     4981 ccccgaccgc cgcgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg
     5041 gatcggcaaa taacgcctgc cagcgtgtca acgacaacgc aggcgatatc agcgtgagag
     5101 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     5161 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     5221 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     5281 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     5341 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     5401 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     5461 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gtgcggggtt
     5521 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     5581 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcta
     5641 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     5701 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     5761 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     5821 ttgtggtggc tgtggcgtaa catcg
//
LOCUS       DAJVPT010000177         5647 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVPT010000177 DAJVPT010000000
VERSION     DAJVPT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..1116
                     /gene="celF"
                     /locus_tag="OQM47_003765"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             1..1116
                     /gene="celF"
                     /locus_tag="OQM47_003765"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1129..1887
                     /gene="chbG"
                     /locus_tag="OQM47_003766"
     CDS             1129..1887
                     /gene="chbG"
                     /locus_tag="OQM47_003766"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1481745.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1934..4195)
                     /gene="katE"
                     /locus_tag="OQM47_003767"
     CDS             complement(1934..4195)
                     /gene="katE"
                     /locus_tag="OQM47_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1481746.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4378..4641
                     /gene="cedA"
                     /locus_tag="OQM47_003768"
     CDS             4378..4641
                     /gene="cedA"
                     /locus_tag="OQM47_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1481747.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4914..5647
                     /locus_tag="OQM47_003769"
                     /pseudo
     CDS             4914..5647
                     /locus_tag="OQM47_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1428 a         1221 c         1434 g         1564 t
ORIGIN      
        1 gatgctgatt tcgttactac ccaactgcgc gttggccaat taccggcgcg tgaactggat
       61 gaacgtattc cattaagtca tggttatctt ggtcaggaaa ccaacggcgc gggcggtttg
      121 tttaaaggtc tgcgtaccat tccggtgatt tttgacatcg taaaagatgt cgaagaactt
      181 tgtccgaatg catgggtgat taacttcact aacccggcgg gaatggttac tgaagccgtt
      241 tatcgtcata ccggatttaa acgctttatc ggcgtgtgta atattccgat cggcatgaag
      301 atgtttattc acgatgttct gatgctgaaa gacagcgatg atttatctat cgatttgttt
      361 ggcctcaacc atatggtgtt cattaaggat gtgctggtaa atggcaagtc gcgctttgcc
      421 gaattgcttg atggtgtggc atccgggcag ttaaaagtat ctggcgttaa aaatattttc
      481 gatctgccat ttagcgaagg cttaattcgt tctctgaatc tgttgccgtg ttcttatttg
      541 ctttattact tcaagcaaaa agagatgctg gctattgaaa tgggcgaata ctacaaaggc
      601 ggcgcatgag cgcaggtagt acagaaagtc gagaaacaac tttttgagct gtataaaaac
      661 ccggagttga aagttaagcc gaaagaactg gaacagcgcg gtggggctta ttactctgat
      721 gcagcgtgcg aagtgatcaa cgctatctac aacgacaagc aagctgaaca ttacgttaat
      781 atcccgcatc atgggcatat tgataatatt ccggcagact gggcggtaga aatgacctgt
      841 aagctggggc gcgatggcgc gacgccacat ccgcgcatta cgcatttcga tgataaagtg
      901 atggggctga ttcacaccat taaaggcttc gagattgctg ccagcaacgc cgcacttagc
      961 ggagaattta acgatgtgtt actggcgcta aaccttagtc cgttggtgca ttccgatcgc
     1021 gatgctgagc tgctggcacg cgagatgatt ctggcgcacg agaaatggct gccaaatttt
     1081 gccgactgca tcgcagagct taaaaaagca cattaaccga ggctgattat ggaacgctta
     1141 ctgattgtta atgccgatga ttttggctta agcaaaggtc agaactacgg cattatcgag
     1201 gcctgtcgca atgggattgt cacatcgacg acggcgctgg tgaatgggca ggctattgac
     1261 catgcggtgc agttgagtcg tgatgaaccg agtctggcca tagggatgca ctttgtcctt
     1321 actatgggca agccactgac agctatgccg gggttaaccc gcgatggtgt gctgggaaaa
     1381 tggatctggc agttggcaga agaagatgct ttaccgctgg aagaaattac tcaggagctt
     1441 gccagtcagt atttgcgttt cattgagcta tttggacgca aacctacgca tcttgatagc
     1501 catcatcatg tgcatatgtt cccgcagatt ttcccgattg ttgccaggtt tgcggctgaa
     1561 gaggggattg cgttgcgcat cgaccgtcag ccgctatcta acgctggtga tttaccggct
     1621 aatctgcgca gttcgcaggg attcagtagc gcgttttatg gtgaagagat tagcgaggca
     1681 ctgttcctgc aagtgctgga tgacgcaagc catcggggtg atccttcgct ggaggtgatg
     1741 tgtcatccgg cgtttatcga taatactatc cgtcagagtg cttactgttt cccacggtta
     1801 acggagctgg aagtgctgac atcagcgtcg ttgaaatatg caattgctga gcgtggttat
     1861 cggttgggta gttatcttga tgtgtaagta aggtggtaag gtcactcccc aggggacgtg
     1921 accttatcca tatttacgcc ggaattttgt caatcttagg aatgcgtgac cacacgcggt
     1981 gttctgccat cagcgttaac agttcatcca taaaactacc gtcggtgctg tcagcttcca
     2041 caatcccttc ttcaccctgg tcagctacct tgattgttgc tttaaacttg cgcgcgtctc
     2101 ccgccagcgc aatcggttta aggtgtttgt aggcttccat cagatagtag ttggcatcgc
     2161 cgttgtcagc gatatccgcg atattgccgc aagggacaat gaccgcatcg accgtcagcg
     2221 aaggtgcacc ggcaaaggta gcggctatag gcaacaccgt accgtcatcc gcagtcactt
     2281 cacccattcg ggagtagagc agtttggcat gaacgccttt ggccttcatg gccttgagaa
     2341 tggccagaag gtctgccgat ctcacttcat cattaagtaa aatcgctacc acgcgacctt
     2401 tcacatcacc gtcaggaatg gcgtacaaac ttaaggatgg atcctttttc agaccgttga
     2461 cgtccggagg tggggtgata ttcagctggt cgtcagtcag ttcgataccg agatttttcg
     2521 ccaccgcctg ggccagagtg agatcaatat gcgccagctg gtcaacaacg cgctcacgaa
     2581 tatacggacg aacgactttg cttaactcaa aactgaaacc atcgacaata tggcgctgct
     2641 cgaatggcgt ctgacttagc cagaacagac gcggatggga ataatattcg ccaaacgatg
     2701 ggctgcgctc gcgaacttta ttgccttcca cgcgctcttg gtatgattca aaaccgccgc
     2761 gtttcggccc cggtggtgtt tcgcgcggcc agttatcgtt gatcgagttc ggttcataat
     2821 tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc acgctggaaa ttatggtaag
     2881 ggcaggtcgg gcggttaatc ggaatctcat ggaaattcgg cccaccaaga cgactgattt
     2941 gtgtatcggt ataggagaac aaacgtccct gcaacagggg atcgttggtg aagtccagac
     3001 caggaacaat atgacctgga tggaatgctg cttgttcgtt ttcggcaaag aagttatccg
     3061 gattgcgatt gagcaccatt ttgccgacac gctgaacggg caccagttct tccgggatca
     3121 gtttggttgg atcgagaaga tcgaagtcga acttgaattc atcttcttca ggaatcaact
     3181 ggaagcccag ttcgtattcc ggaaaatcgc ctgcttcaat ggcttcccac aactcgcggc
     3241 ggtggaagtc cgggtcacgt ccggtgagtt tttgtgcttc atcccaaacg agtgaggctt
     3301 tacctgccag tggtttccag tggaaacgta caaacgttgc cttcccttcg gcattaatca
     3361 ggcggaaggt gtgaataccg aagccttcca tggtgcggta actgcggggg atgccgcgat
     3421 ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca taatcccaga
     3481 aagtatcgtg ggcactttgc ccttgtggaa ttgcccagtg cggttctggt tttaccgcat
     3541 gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg ttattgccaa
     3601 cgaggtcaaa aataccctct tcagtataga acttggtggc aaaaccacgg atatcacgca
     3661 cggtatcggc agagccagcg ccaccctgaa cggtagagaa cctaacaaat actggggtga
     3721 ttttgttcgg atctgagagg aaatccgctt tggtgatatc gcttaagctt ttatatggct
     3781 ggaaataacc gtgagctgct gagccgcgtg catgaacaat acgttccggg atgcgctcat
     3841 ggtcaaagtg ggtgattttc tcgcgcagaa taaaatcttc cagcagcgtt ggaccacggt
     3901 taccggcacg cagtgagttt tgatcatcgg cgatgcgcac gccctgatta gtggtcagcg
     3961 cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg ttacgtgtat
     4021 caggggcttt caggctccca ggggcggtag gttgtgcacc tggcggtgtt ggttcagccg
     4081 ctggacgatg agatccgtcc tcaggtgcca gtgagtccat ccccggtttc gcttcgctgg
     4141 aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc gacattgaac
     4201 tcgtctcctt aatttattac tgaaagggcc gctgttttgg tcaaaaaact ctctaactat
     4261 agaccaccag ccagatcccg cttcggagac tattctggaa acggtgttat gaaatacacg
     4321 caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctaat ttttaatatg
     4381 cgtttagtga agccagtaat gaagaaaccg ctccgccagc aaaaccgcca gattattagc
     4441 tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga ttcgtttcgt
     4501 gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatgggaga gtcatttctg
     4561 cgctcaccgg cgtttactgt gcctgaatct gcccaacgtt gggcaaatca gatccgccag
     4621 gaaggggaag tgactgagta aggattacat acctcccggt tttgccggga gttacaggac
     4681 ctgaaattct gcatttcgtt gctacattca ttcaaaccac ctgtaacaaa aagctgcctt
     4741 tgacgagcta ttgctataaa taagagatct tttttcttta attatttatt ttaaaatggt
     4801 taagttttgc taatcatgat gagttatttt tgatagttca tcctgtggcg tcgctcttaa
     4861 acttatattg taaatgactt agatttaaat aagataataa ttttgttaac aatatgaggc
     4921 gggagatgtt ttctacaaac ttaatacaga gtaattatgg tgatttaaat attaaaagcc
     4981 tggctttcga ctcttttaaa gaaaggcttg agtctaccat ggcggcactc actttcttta
     5041 tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc gaacttcaat cgtatgattg
     5101 cctatatgtc taacattaac tatgatgcct caaagctagg agcgccagca cttagttttg
     5161 atacgtatct gcaggacaat gttaaatata gagtgataat taataatctt tatggcagtg
     5221 agtttcggat aagagatatt aatgagaatt ttgttgggat ggattttacc tgtgaattcc
     5281 gacccgaaaa aatgacgaat ttgatcaata taaaaaatag gttggttatt cattatttaa
     5341 ggactatata ctatgaaaaa aattatatac atcctgccgg gagcattttt gctgcgattc
     5401 aaaaaaaatg agagtctcct aaagtttcct tcgataatat caatgcttaa tatcaatctt
     5461 ctttttaatc cactaaatct tccgggaatg ggaagtggtg tgcttgaaga tattatgagt
     5521 attccagata gttcacttag aaaacgtctc gggtatgagg ttttaagctt ctcactacag
     5581 gcacattcat tgagccagga atgtattgat aagttggaca tttttttcgc tgacgatctg
     5641 tttaaat
//
LOCUS       DAJVPT010000178         5581 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVPT010000178 DAJVPT010000000
VERSION     DAJVPT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            27..242
                     /locus_tag="OQM47_003770"
     CDS             27..242
                     /locus_tag="OQM47_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1481748.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            235..621
                     /locus_tag="OQM47_003771"
     CDS             235..621
                     /locus_tag="OQM47_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCS1481749.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            629..1315
                     /locus_tag="OQM47_003772"
     CDS             629..1315
                     /locus_tag="OQM47_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1481750.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OQM47_003773"
     CDS             complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OQM47_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1481751.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1998..3203
                     /gene="tet(B)"
                     /locus_tag="OQM47_003774"
     CDS             1998..3203
                     /gene="tet(B)"
                     /locus_tag="OQM47_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1481752.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OQM47_003775"
     CDS             complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OQM47_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1481753.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4042..4413
                     /locus_tag="OQM47_003776"
     CDS             4042..4413
                     /locus_tag="OQM47_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1481754.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4423..>5581)
                     /locus_tag="OQM47_003777"
     CDS             complement(4423..>5581)
                     /locus_tag="OQM47_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1481755.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1029 c         1215 g         1795 t
ORIGIN      
        1 cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt ctagctacag
       61 aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg caatggaaaa
      121 atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt gatttttaca
      181 aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag gataatgttt
      241 gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg aaaaacatta
      301 ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga tgaatgccat
      361 gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca agctggactg
      421 ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa accgggggaa
      481 cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt gcaatacagt
      541 gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc atctgttacg
      601 ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag aggttacctt
      661 tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact
      721 cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat
      781 atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt
      841 agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat
      901 ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt
      961 gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat
     1021 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
     1081 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
     1141 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
     1201 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
     1261 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
     1321 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1381 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1441 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1501 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1561 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1621 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1681 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1741 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1801 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1861 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     1921 aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag
     1981 tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg
     2041 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     2101 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     2161 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     2221 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     2281 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2341 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2401 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2461 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg
     2521 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2581 ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga
     2641 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2701 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2761 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2821 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2881 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2941 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     3001 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     3061 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     3121 tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga
     3181 gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat
     3241 aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa
     3301 taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat
     3361 aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat
     3421 aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc
     3481 ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt
     3541 ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt
     3601 attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt
     3661 tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca
     3721 ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa
     3781 cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat
     3841 tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt
     3901 attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg
     3961 tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc
     4021 tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg
     4081 gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat
     4141 gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta
     4201 acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag
     4261 ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa
     4321 caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt
     4381 aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc
     4441 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
     4501 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
     4561 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
     4621 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
     4681 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
     4741 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
     4801 tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga
     4861 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
     4921 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
     4981 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
     5041 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
     5101 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
     5161 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
     5221 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
     5281 gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca
     5341 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
     5401 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
     5461 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
     5521 tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
     5581 t
//
LOCUS       DAJVPT010000179         5581 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVPT010000179 DAJVPT010000000
VERSION     DAJVPT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(106..1815)
                     /locus_tag="OQM47_003778"
     CDS             complement(106..1815)
                     /locus_tag="OQM47_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1481756.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OQM47_003779"
     CDS             complement(2247..2966)
                     /locus_tag="OQM47_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1481757.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="OQM47_003780"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="OQM47_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="OQM47_003781"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="OQM47_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="OQM47_003782"
     CDS             complement(3998..4576)
                     /locus_tag="OQM47_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481758.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5234)
                     /locus_tag="OQM47_003783"
                     /pseudo
     CDS             complement(4997..5234)
                     /locus_tag="OQM47_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5421..>5581)
                     /locus_tag="OQM47_003784"
     CDS             complement(5421..>5581)
                     /locus_tag="OQM47_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1481759.1"
                     /translation="TRRGMYQQCLKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEP
                     LARIRWCG"
BASE COUNT         1573 a         1223 c          968 g         1817 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccctcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     5281 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     5341 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     5401 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     5461 ataacctgct ctgccagacg gtaacccctc ttcagccccg taaaccgcat ctgacctccg
     5521 cgcagctcgc acttcagcgg gtcaaccttc agacactgct gatacatccc tctccttgtg
     5581 a
//
LOCUS       DAJVPT010000180         5548 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVPT010000180 DAJVPT010000000
VERSION     DAJVPT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(150..511)
                     /locus_tag="OQM47_003785"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="OQM47_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="OQM47_003786"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="OQM47_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="OQM47_003787"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="OQM47_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OQM47_003788"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OQM47_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1481760.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="OQM47_003789"
     CDS             complement(2779..3093)
                     /locus_tag="OQM47_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481761.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="OQM47_003790"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="OQM47_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OQM47_003791"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OQM47_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1481762.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="OQM47_003792"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="OQM47_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="OQM47_003793"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="OQM47_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1510 a         1203 c         1106 g         1729 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacataga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tatcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactt
//
LOCUS       DAJVPT010000181         5408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVPT010000181 DAJVPT010000000
VERSION     DAJVPT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..218
                     /locus_tag="OQM47_003794"
     CDS             <1..218
                     /locus_tag="OQM47_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1481763.1"
                     /translation="RTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGIDPEAYLR
                     HILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            250..864
                     /gene="istB"
                     /locus_tag="OQM47_003795"
                     /pseudo
     CDS             250..864
                     /gene="istB"
                     /locus_tag="OQM47_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1033..5127)
                     /gene="sepA"
                     /locus_tag="OQM47_003796"
     CDS             complement(1033..5127)
                     /gene="sepA"
                     /locus_tag="OQM47_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCS1481764.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1322 a         1363 c         1271 g         1452 t
ORIGIN      
        1 ttcgtaccgt ctgtctcggg aaaaagaatt acatgttctt cggcagcgat catggaggcg
       61 atcgcggagc tctgctgtac ggactaatcg ggagctgtcg gttgaacggt atcgatccag
      121 aagcgtatct gcgccatatc ctgagcgtac tgccggaatg gccttccaac cgagttgacg
      181 aactgctgcc atggaacgta gttctcaccg ataaataagc gtcaatacgg ccttccgttg
      241 acgcttacag aactcctgtg gcgcgagagt gaaaaactgc gtcgttataa aaaggaggcc
      301 cggttgccag ttgccaaaac gctaagcgaa tacgacttca ttcagttgcc ggaactgaat
      361 ggagctcagt tccagcaact ctgtgaaacg acagactggg ttgatgctgg agaaaacgtt
      421 ctgctgttcg gggccagcgg gttggggaaa agccatctgg cggcagcgat cgtggatggc
      481 gtagtaggcc agggctaccg ggcccggttc tacagcgcag gagagctgtt gcaggaacta
      541 cgtaaagcca gagctcagtt gaaactgaat gagctgctac tgaaactgga tcgctaccgg
      601 gtgatagtgg tggatgatct tggctatgtc aaacgcgaca gcgccgaaac gggtgtgctg
      661 ttcgagttaa tagcgcatcg ctatgaacgt gggagcctgg tgataaccag taaccatccg
      721 ttcagcatgt ggggcagcat cttcgtggat gagactatgg cggtggcggc agcagaccga
      781 ctgatccatc acggatatat gttcgaactg aaaggggaaa gctacaggaa aaagacagcg
      841 aaggcagtaa caagcgtgac ttgatgtcgc actgaagggg cggccagtat agttggcgcc
      901 agtcggcaaa actagttgac gtctaatacc tggacggtca cttctgagaa aaggcattta
      961 cacagaatct taaacaggct cacggagttc tttcagaaaa aggcctgccg aatggcagac
     1021 ctatcccatt cgtcagaaaa cataacggaa gttggcgttt atcgcattat ccacattata
     1081 tttcccgaac gctgatttct ccagctccag tcccaaacgc atattatcct taatttccgc
     1141 attcatccct accgtcatca gcatcctgct atctttttca ccttcgaaac gtttctctcc
     1201 ggaagcatcc tgcagaaccg tttctccgtt cgccagcagg tcgaactgat aacccagccc
     1261 ggctcgagct gtgattttcc agtcgtctcc ggagaaggct cttcccacgt caacaccagt
     1321 acggccaatc agtgggttat aatccttgtc tttcatgctc agagccattc cccggtcttc
     1381 ccagctaaaa gcttttcctg atacagaacc gtaaaccagc tctatctgtg gctccaccca
     1441 ggactctttc gtcaggtggt aacgataacc aacttcagca ccggcatacc aggaatgaga
     1501 gctgtagtct tttgttccca gtgaggcaaa attcgccgtg tgctgattat catgatggag
     1561 atatttacca atcaggtcaa aataagctcc ggaattaaag agtgcagagg catacagacc
     1621 gccacccacg gatttgtttt ttccactgaa tgcgtggctg cttgcattgc tgtccgtata
     1681 ggtcagcaat gcccccgtaa ataaatccac accgtccagc tcatgctttc tgtcgacacc
     1741 aatctgaacg tgagtgtagt tatcgctgta gtcaccatct gccgaaccgg taccattcat
     1801 tatgcgtgcc cagacaccgg catccccctg agtatcccgc aggtcaccca tacgtttgtt
     1861 caggttattg acctctgtaa ggaaggactt ataacctgat gccataaagt cttttgcggc
     1921 tttcgaggct ccggcatcag cggtagtctg atatcctgtc agcacccatt ttgtggcatc
     1981 atccgttttt tccgtgctga ttactggcgt aacgttactg aaaccaatct gctgcgtacc
     2041 ggcagtgaac gttttctcat cactcccttt cggtgcggta attaacgtca cgctcatttc
     2101 actggctggt ttgtttaaaa agtcaaccag gacgctatta ttactgcctg acagtttgtc
     2161 tgtgacattc aactggtcag cccggctgtt gttggtatgc atcaggaacg tggtattatc
     2221 tgcagaaagc tcttttactt gtaaagtggc aaagtgtttc ccgtccccca gttcaattcg
     2281 cgagccctta gccttcagtg caccggcctc tgattttccg tcagagtacc acagagcatt
     2341 gatcatattt accgtactct gtgcccccgt aatactgcct ctcagaacag cgttatatcc
     2401 cttcattaag gagctgaata acggagagtc cgtcttccct gcatcagcat cactgtctcc
     2461 cagattaatg gttgccgcag tggttcctgc atgaatatcc gaactcagat agcccatatt
     2521 tttaacagaa aagctggcgt tatcttcgag gttaatccct tctgttgtag aaattacggg
     2581 tgagtaatac ccctgtttct gcgcagaagg catccccgtc agggtcagag aagcattttc
     2641 ttttacatcc acagccccgg tgctgaaaat accgctctga gccgtaagtt ttgctccttt
     2701 tccgagggtc aggctgctgt tgacaaagct gccaacacgg tcaaaaacag cattctgaga
     2761 ggtcacactc acggtgctat cataagcctc aataccaccg gtgaatctct ccatgatttg
     2821 cagggatgat ttattttcca gcgtaacatt tccgctgtaa tcactctggt caccaactgt
     2881 ggttgcagtg gcactccctt caacagagga gaccgcatta ccggttccgt cactcctgtc
     2941 agtaaagaca cggctacttc ccagaatgac tgtacttcca ttggtggcgt taatatctcc
     3001 ctgcatcttg ccgtttctgg acagatggaa tgttgccgca tcgagtttca gtttttccag
     3061 cgtaaactga cgggtttccc agtcgctctg gctggaagaa gtcgtcgttc cagcatgaat
     3121 aactggatga ccctggaata tcagcgtgcc ggacttcttg gttatcgaac cttctggcag
     3181 gttaactgaa ccatcaagag ccagtacatc cttacctgag agctgcggaa tattcacatc
     3241 catattgccg tttagttggc cgtgatacat ataaggctgg ctgctggcct caattttttg
     3301 ttgcttcaca gtatcaatgg ctttattacg atcttttccg acattctgcc agacactgct
     3361 gttgttcaga tcggagaaga acggggaata tgaactcgct ttcaggatga aatattgctt
     3421 ggtagaactg tcataatata ggtctcccgg cgctcctctc cccccaaaaa tactgacagt
     3481 attaaccggt acattgatgt cactggcttt taatgtctga aggtcgagtg ttaccgttga
     3541 ttttttctca ctgttattgg taatgatggc cccgtagtca gccgcctgaa gacgggtaaa
     3601 ggtcagatta ttaccattca gttccagacg tcctccacgg tatccccacg aaatattatc
     3661 cggattaact tgctgtgagt cagaaagcac aactgtcgga cgaccgctgg caataccaac
     3721 agagctgaaa gcctgtacct ttccgtcagc atccgcctgc tggttgagaa taacggtacc
     3781 gtcccccacc ttgagtccgc ctgcattcac accggtcccg tttactgtca gggtaccttc
     3841 gccagtttta tgcaggttat caccggcaac tccgttcacc tgccacagaa cgtgggttcc
     3901 tttatcggtg ataatcccgc cccccatcca tgtctggccg ttcagggcgg acaccctgta
     3961 atcgccggca aactgcagat acccagctcc ctgattaacg gaattctgca gaacgacctc
     4021 gccattattg ccggtgaaca ggaggttttt tcctgcgttc agatcatttc ctttctttcc
     4081 atgcatatcc caagtcgtgt ttccctgagt cagtgtgccg gtgccattag ccgcatcata
     4141 tttccactgc agtactcctt caccagaggt gtaggctata gttttgtcaa aatcattttg
     4201 cggttgctga ccaaggaaat cctgagtggt gacgacccag ttatttccat agttagttcc
     4261 tgaggaaagc acgccaatca gtacccattt tttttgcagg gaatcataag caaacagggg
     4321 ggagccgctg tcccccatat tcagataatt agccagaggt ccattcgcgg gattaaaaat
     4381 gtcaccggtc tgagctgtaa tcatctgtcc gttgttatag gagttcagcg cgctgacggt
     4441 tcccccaaca agaaatccgc cattggtata cacttgggtg cgattaccat ttttgtcttt
     4501 gacgtactgc atcccccccc caaggcgata gaattcagta aagcgtccgc cggcctgata
     4561 ggcaccgctc acagccccga tgtcagacac ttctgccggc gccacctctg tcaccagttt
     4621 actcaggcgg cgggttttaa tatcaagacc gctattatta tttgtaccaa ccgcagtata
     4681 ggtgtttttg gcgtatccga agctcatcgt gtcagaaccg ctgacatgtt tggcggtaac
     4741 cacatactga ggggagtaaa gtgtatggtc tcccggaggc aggctaccgg tagtaatagt
     4801 ggcactactg aaatcagcca tcggggcttt atcaagttta ccgaccagat tgccctgctt
     4861 gtcgtaaatg gaaatattcg tggtccccgg cgtaaactga cctttattct cggcaaagtc
     4921 gcggaatatc tgataaggga tctctgcact gaccgttgct gacagagctg gccatgccga
     4981 ggaaagtgat aaagctgcaa cagacgtaag aataacccga cgtgaaagtc tgcgatggct
     5041 tttgcaggta accctgcggg ccagttcgga gacagcaatc aggcttttgg ttatatggca
     5101 atacttaaga taatatattt tgttcatata gaaacatcaa cctaattttg acaaagatta
     5161 attacatagg atgtagatgg agcagtccga gattctacta atatcaatta ttaagcatct
     5221 gttttttagt tggtgtaata taatttttat taaggtaagc ggaaaaacaa ccaaaaaaaa
     5281 cgcaagatgt tattttttta tcattttgag ccagtttggc tggggggata ctcttctctt
     5341 aagcttcttg ctcaacttta aaaatagtta tgccagttcg gtatcgataa ggggcagttt
     5401 ggatatcg
//
LOCUS       DAJVPT010000182         5357 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVPT010000182 DAJVPT010000000
VERSION     DAJVPT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..231
                     /locus_tag="OQM47_003797"
     CDS             <1..231
                     /locus_tag="OQM47_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481765.1"
                     /translation="FYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTVG
                     KREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(228..1169)
                     /locus_tag="OQM47_003798"
     CDS             complement(228..1169)
                     /locus_tag="OQM47_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1481766.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1181..1670)
                     /locus_tag="OQM47_003799"
                     /pseudo
     CDS             complement(1181..1670)
                     /locus_tag="OQM47_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1674..2894)
                     /locus_tag="OQM47_003800"
     CDS             complement(1674..2894)
                     /locus_tag="OQM47_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1481767.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2898..3641)
                     /locus_tag="OQM47_003801"
     CDS             complement(2898..3641)
                     /locus_tag="OQM47_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1481768.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3532..4998)
                     /locus_tag="OQM47_003802"
     CDS             complement(3532..4998)
                     /locus_tag="OQM47_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1481769.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4998..5231)
                     /locus_tag="OQM47_003803"
     CDS             complement(4998..5231)
                     /locus_tag="OQM47_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1481770.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
BASE COUNT         1136 a         1507 c         1357 g         1357 t
ORIGIN      
        1 ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt gcgcacgagt
       61 gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa actcgcgccg
      121 agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg catcattttt
      181 tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta aatgccatca
      241 acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc gtagtaagtg
      301 acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac cagcgggtag
      361 cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc cacaccacgg
      421 cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg
      481 gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac gatgatgctg
      541 gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac agagtaacca
      601 gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt
      661 gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct
      721 ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat ctgccatttc
      781 agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg agcagcctgc
      841 aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt accggatttg
      901 ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca gttttttcca
      961 ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca
     1021 tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta ccccaccagt
     1081 ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc accagagctg
     1141 tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt
     1201 ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc accacgtttt
     1261 tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat tcatcagtgg
     1321 agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac ggtcacgtag
     1381 ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg aaccatgtcc
     1441 ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc tgcggtatca
     1501 ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag gccataggca
     1561 gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg cgggcgagag
     1621 acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat tttttaccct
     1681 ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact ggtcatgccc
     1741 atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt ggcaacctca
     1801 gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat atccgcatca
     1861 ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg tttgaacgcg
     1921 gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc acgccaggct
     1981 gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt cggattatcg
     2041 tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag cagggctttc
     2101 agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat ctctggtgct
     2161 ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc ctgcggcaaa
     2221 tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt catggtgtcg
     2281 gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt ttgatttgcc
     2341 attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg gacaagagcc
     2401 acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg ctcgtactcc
     2461 gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat ggcgctttcg
     2521 tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc gcgccggaca
     2581 ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag ccgcacattc
     2641 ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga ggcaagcgtg
     2701 gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa cttatcgggt
     2761 ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc tatcggcacg
     2821 tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag cctggtctca
     2881 aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg aacaaacacg
     2941 atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat gccgtccaaa
     3001 gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga atctcggaca
     3061 tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat cgctcgggcc
     3121 atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc ggccctagac
     3181 ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg accaccggtg
     3241 acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc ctcgagaggc
     3301 agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg gcccacagga
     3361 gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg ccgccaaact
     3421 ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt cgcccagggg
     3481 gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact gtcatttgaa
     3541 ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg taggctggtt
     3601 cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc cgatgccggg
     3661 cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc ctatgccggt
     3721 tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca gaccatcacc
     3781 cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat cgacatcaga
     3841 catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct tgccaagttc
     3901 cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc tctcctgcaa
     3961 cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa atcctttcgg
     4021 ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct gttctgcaaa
     4081 ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa acttatcccg
     4141 cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt cgatattctt
     4201 cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct tctccacgct
     4261 gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag cgctgtcgaa
     4321 cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt tctcggtcat
     4381 cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt gatgaatgcg
     4441 ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt tacccaggtc
     4501 cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc ggtcgagcgg
     4561 gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg tcattggtgc
     4621 ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc atttcagcac
     4681 gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa tgccacgatc
     4741 ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat ccgggatagc
     4801 atcgactgct tcgccgatga tccaggatga acgataggac cattccacca gcatgcggtt
     4861 acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg tctgcatccc
     4921 tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc gccgcagggc
     4981 ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc cagcgatgta
     5041 tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg tatctcgcgt
     5101 tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa atcacgatga
     5161 ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc tatgctgatc
     5221 agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt tctgaaacgg
     5281 tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg atgccgttgt
     5341 actcgtcatc tggctca
//
LOCUS       DAJVPT010000183         5302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVPT010000183 DAJVPT010000000
VERSION     DAJVPT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..397
                     /locus_tag="OQM47_003804"
     CDS             <1..397
                     /locus_tag="OQM47_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCS1481771.1"
                     /translation="AMAGMQTRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDR
                     AEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGK
                     SMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            419..2410
                     /gene="cyoB"
                     /locus_tag="OQM47_003805"
                     /pseudo
     CDS             419..2410
                     /gene="cyoB"
                     /locus_tag="OQM47_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2400..3014
                     /locus_tag="OQM47_003806"
     CDS             2400..3014
                     /locus_tag="OQM47_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1481772.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3014..3343
                     /locus_tag="OQM47_003807"
     CDS             3014..3343
                     /locus_tag="OQM47_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1481773.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3355..4245
                     /gene="cyoE"
                     /locus_tag="OQM47_003808"
     CDS             3355..4245
                     /gene="cyoE"
                     /locus_tag="OQM47_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1481774.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4429..4740
                     /locus_tag="OQM47_003809"
     CDS             4429..4740
                     /locus_tag="OQM47_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1481775.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4724..4882
                     /locus_tag="OQM47_003810"
     CDS             4724..4882
                     /locus_tag="OQM47_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1481776.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4875..5273
                     /locus_tag="OQM47_003811"
     CDS             4875..5273
                     /locus_tag="OQM47_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1481777.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1156 a         1314 c         1406 g         1426 t
ORIGIN      
        1 tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc aacgaacccg gtacttatga
       61 cggtatctcc gccagctaca gcgggccggg cttctcaggc atgaagttca aagctattgc
      121 aacaccggat cgcgccgaat tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac
      181 catgtctgac atggctgcgt tcgaaaaact ggccgcgcct agcgaataca accaggtgga
      241 atatttctcc aacgtgaaac cagacttgtt tgccgatgtg attaacaagt ttatggctca
      301 cggtaagagc atggacatga cccagccaga aggtgagcac agtgcacacg aaggtatgga
      361 aggcatggac atgagccacg cggaatccgc ccattaaagg ggttgaggaa gaataaagat
      421 gttcggaaaa ttatcacttg atgcagtccc gttccatgaa cctatcgtca tggttacgat
      481 cgctggcatt attttgggag gtctggcgct cgttggcctg atcacttact tcggtaagtg
      541 gacctacctg tggaaagagt ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta
      601 tatcatcgtg gcgattgtga tgttgctgcg tggttttgct gacgccatta tgatgcgtag
      661 ccagcaggct cttgcctcgg cgggtgaagc gggcttcctg ccacctcacc actacgatca
      721 gatcttcacc gcgcacggcg tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg
      781 tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct
      841 caacaactta agcttctggt ttactgttgt tggcgtgatt ctggttaacg tttctctcgg
      901 cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca ccgctatcgg gaatagagta
      961 cagtccggga gtcggtgttg attactggat atggagtctc cagctatccg gtataggtac
     1021 gacgcttacc ggtatcaact tcttcgttac cattctgaag atgcgcgcac cgggcatgac
     1081 catgttcaag ataccagtat ttacctaggc atcactgtgc gcgaacgtac tgattattgc
     1141 ttccttccca attctgacgg ttaccgtcgc gttgttgacc ctggatcgct atctgggcac
     1201 ccatttcttt accaacgata tgggtggcaa catgatgatg tacatcaacc tgatttgggc
     1261 ctggggccac ccggaagttt acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat
     1321 tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc tcgctggtat gggcaaccgt
     1381 ctgcatcacc gtgctgtcgt tcatcgtttg gctgcaccac ttctttacga tgggtgcggg
     1441 cgcgaacgta aacgccttct ttggtatcac cacaatgatt atcgccatcc cgaccggggt
     1501 gaagatcttc aactggctgt tcaccatgta tcagggccgc atcgtgttcc attctgcgat
     1561 gctgtggact atcggtttta tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct
     1621 ggcagtaccg ggcgcggact tcgttctgca taacagcctg ttcctgattg cgcacttcca
     1681 taacgtgatc atcggcggcg tggtcttcgg ctgcttcgca gggatgacct actggtggcc
     1741 taaagcgttc ggtttcaaac tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat
     1801 catcggcttc ttcgttgcct ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg
     1861 tcgtttgagc cagcagattg acccgcagtt ccacaccatg ctgatgattg cagccagcgg
     1921 tgcagtactg attgcgctgg gtattctctg cctcgttatt cagatgtacg tttctattcg
     1981 cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg ggtggccgta cgctggagtg
     2041 ggcaacctct tccccgcctc cgttctataa ctttgccgta gtgccgcacg ttcacgaacg
     2101 tgatgcattc tgggaaatga aagagaaagg cgaagcgtat aaaaagcctg accactatga
     2161 agaaattcat atgccgaaaa acagcggtgc aggtatcgtc attgcagctt tctccaccat
     2221 cttcggtttc gccatgatct ggcatatctg gtggctggcg attgttggct tcgcaggcat
     2281 gatcatcacc tggatcgtga aaagcttcga cgaggacgtg gattactacg tgccggtggc
     2341 agaaatcgaa aaactggaaa accagcattt cgatgagatt actaaggcag ggctgaaaaa
     2401 tggcaactga tactttgacg cacgcgactg cccacgcgca cgaacacggg caccacgatg
     2461 caggcggaac caaaatcttc ggattttgga tctacctgat gagcgactgc attctgttct
     2521 caatcttgtt tgctacctat gccgttctgg tgaacggcac cgcaggcggc ccgacaggta
     2581 aggacatttt cgaactgccg ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca
     2641 tcacctacgg catggcggct atcgccatgt acaaaaacaa caaaagccag gttatctcct
     2701 ggctggcgtt gacctggttg tttggtgccg gatttatcgg gatggaaatc tatgaattcc
     2761 atcacctgat tgttaacggc atggggccgg atcgcagcgg cttcctgtca gcgttctttg
     2821 cgctggtcgg cacgcacggt ctgcacgtca cttctggtct tatctggatg gcggtgctga
     2881 tggtgcaaat cgcccgtcgc ggcctgacca gcactaaccg tacccgcatc atgtgcctga
     2941 gcctgttctg gcacttcctg gatgtggttt ggatctgtgt gttcactgtt gtttatctga
     3001 tgggggcgat gtaatgagtc attctaccga tcacagcggc gcgtcccatg gcagcgtaaa
     3061 aacctacatg acaggcttta tcctgtcgat cattctgacg gtgattccgt tctggatggt
     3121 gatgacaggg gctgcctctc cggccgtaat tctgggaaca atcctggcaa tggcagtggt
     3181 acaggttctg gtgcatctgg tgtgcttcct gcacatgaat accaaatcag atgaaggctg
     3241 gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct atcctggttg taggctccat
     3301 ctggattatg tggaacctca actacaacat gatgatgcac taagagcggc ggttatgatg
     3361 tttaagcaat acctgcaagt aacgaaacca ggcatcatct ttggcaacct gatctcggtg
     3421 attgggggat tcctgctggc ctcaaagggc agcattgatt atcccctgtt tatctacacg
     3481 ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg
     3541 gatatcgaca gaaagatgga aaggacgaag aatcgggtgc tggttaaagg cctgatctct
     3601 cctgctgtct cgctggtgta cgccaccttg ctgggtattg ctggctttat gctgctgtgg
     3661 tttggcgcga atccgctggc ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc
     3721 gtctatagcc tgtacatgaa acgccactca gtctacggca cattgattgg ttcgctctcc
     3781 ggcgctgcgc cgtcggtgat tggctactgt gcagtaaccg gcgagtttga tagcggcgca
     3841 gcgatcctgc tggctatctt cagcctgtgg cagatgcctc actcctatgc catcgccatt
     3901 ttccgcttta aggattacca ggcggcaaac attccggtat tgccagtggt aaaaggcatt
     3961 tcggtggcga agaatcacat cacgctgtat atcatcgcct ttgccgttgc cacgctgatg
     4021 ctctctcttg gcggttacgc tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc
     4081 tggtggttag gtatggctct gcgcggttat aaagttgctg atgacagaat ctgggcgcgc
     4141 aagctgttcg gcttctctat catcgccatc actgccctct cggtgatgat gtccgttgat
     4201 tttatggtac cggactcgca tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt
     4261 aaaaaggtgc tacggcacct ttttattgtc tgagatcaga gatacggcta ataaccgtat
     4321 ctcttgacag agtgagcaga tgcacataaa ttaatcatac ggttgatagc cgtattatgt
     4381 atgagatcga ctccctacga tttcattcag ttaatcaaag aagtcataat gcctggaaaa
     4441 acagccgctc tatacgatgt cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact
     4501 agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag ccgttggggt agatttaagc
     4561 gcttttattt tgagcgctgc tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc
     4621 cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac tcatcgctga acctgctcaa
     4681 ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga
     4741 agagattact ctgcccatag tcctctcctg taattatcag tctgatatta cttatcctgg
     4801 gcaaaaacag tttgattgcg gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat
     4861 cgctaaagaa aagtgtgcgt aatagcgact gtgcggctaa agcacttatt gacagacaaa
     4921 gtggtgaact gattggcatc tgtactttta cggcatattc gctggaaaga caacgcgttt
     4981 ctggcgtcct tcagggttca caaccttcag aaattggtgt tgtcagatta gtcatgttgg
     5041 ggggagcgcg gaagtatcaa aagcggggct ttggtcagga cctactatgt gatttttttg
     5101 aacatgtaaa aaaaattcac ccggcattac caattaaagg ggtttatctt gatgctgacc
     5161 ctgccgccat taacttttat gctcgtctcg gctttgttca gctttcagcg acaccaaatg
     5221 cttttggtgc tgtacctatg tttttggcga ttcagcatat tctcgcggct tagattatta
     5281 tcgccacccg ccagggcaag tt
//
LOCUS       DAJVPT010000184         5191 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVPT010000184 DAJVPT010000000
VERSION     DAJVPT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..497
                     /gene="narU"
                     /locus_tag="OQM47_003812"
     CDS             <1..497
                     /gene="narU"
                     /locus_tag="OQM47_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1481778.1"
                     /translation="GAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGS
                     GNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTET
                     AATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRK
                     FSQK"
     gene            579..4319
                     /gene="narZ"
                     /locus_tag="OQM47_003813"
                     /pseudo
     CDS             579..4319
                     /gene="narZ"
                     /locus_tag="OQM47_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            4316..>5191
                     /gene="narH"
                     /locus_tag="OQM47_003814"
     CDS             4316..>5191
                     /gene="narH"
                     /locus_tag="OQM47_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1481779.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1243 a         1406 c         1416 g         1126 t
ORIGIN      
        1 ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg
       61 tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac
      121 cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga
      181 ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa
      241 ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga gaagccgtca
      301 ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc ggctttttta
      361 ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag
      421 tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga
      481 agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat ccgacaaggc
      541 atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct
      601 ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata
      661 gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt
      721 ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc
      781 tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc
      841 atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc
      901 gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaaac
      961 aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa aagtgcctga
     1021 gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc
     1081 agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg
     1141 gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt
     1201 cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg ccgcccgcct
     1261 cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca
     1321 gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac gcccacttct
     1381 ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag
     1441 tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga
     1501 tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta
     1561 tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag cctcgcgacg
     1621 atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg
     1681 aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc
     1741 cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg
     1801 ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag
     1861 gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca
     1921 atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct
     1981 gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg
     2041 aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg
     2101 agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata
     2161 ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt aaccactggt
     2221 atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg
     2281 ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg
     2341 gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag atgaacagca
     2401 cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt
     2461 tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc
     2521 gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga
     2581 ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga
     2641 aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta
     2701 atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac gagtatatac
     2761 agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg
     2821 ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc
     2881 tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca
     2941 ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca tttattcatc
     3001 cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa atctacaaag
     3061 gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa accgacgtgg
     3121 tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt gaagtgctcg
     3181 actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat attgtggcgg
     3241 tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca
     3301 aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg
     3361 gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg
     3421 ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag
     3481 tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc
     3541 acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct
     3601 ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca
     3661 acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc
     3721 atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc
     3781 gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa gcacttaact
     3841 tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc
     3901 tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga
     3961 ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg actgcccgcg
     4021 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
     4081 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
     4141 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
     4201 ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc cgcaagatga
     4261 agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
     4321 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
     4381 ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
     4441 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     4501 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
     4561 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
     4621 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
     4681 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
     4741 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
     4801 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
     4861 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
     4921 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
     4981 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     5041 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     5101 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     5161 agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAJVPT010000185         5190 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVPT010000185 DAJVPT010000000
VERSION     DAJVPT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            486..1277
                     /locus_tag="OQM47_003815"
     CDS             486..1277
                     /locus_tag="OQM47_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481780.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1663..1869
                     /locus_tag="OQM47_003816"
     CDS             1663..1869
                     /locus_tag="OQM47_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1481781.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1955..2527
                     /locus_tag="OQM47_003817"
     CDS             1955..2527
                     /locus_tag="OQM47_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481782.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2529..2813)
                     /locus_tag="OQM47_003818"
     CDS             complement(2529..2813)
                     /locus_tag="OQM47_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481783.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2784..2927
                     /locus_tag="OQM47_003819"
                     /pseudo
     CDS             2784..2927
                     /locus_tag="OQM47_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4096..5115
                     /locus_tag="OQM47_003820"
     CDS             4096..5115
                     /locus_tag="OQM47_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1481784.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         1667 a         1148 c          981 g         1394 t
ORIGIN      
        1 ggactacatt acagcagaga ggggtaagga cagagttaca agtactacta gagagaatat
       61 cactgcacag atacttctat gagtatatct ttatgagaga tccgtctaca ggattgggtt
      121 ataaggtgta tttacaaggt ataatcacag gatcacttac atgaccatac ttctgtaaat
      181 aatactaatt aaccttaatt atttttttat ggtacatgaa ctacctgtag ctgtaacgat
      241 aaacaaatca cccaaaatga aaaatactgg ttatcacagt tcagagaact cgtatgagtg
      301 tccccctgag caatgcttca ttttctgtga ttctgtgtga tagagttata ctgagtacag
      361 tggcagtaac actgctttcc atcacttgag gataaaattc attaaaaaat taattgcatg
      421 aattaacagg tttttttatg aaatgttatg catctcaacc aatatgatta acagaaaact
      481 tcatcatgac aacacgtata acgttcagta tgaccagtga tgagacgctg cgaattgtgg
      541 atgagtactg tcatacccac aaactttcgc gctctaaggt aatcaacgca ttactcagcg
      601 ccacagcacc tgtccttaat gatattaact gttattatca acttgcaggc gaactgcagt
      661 cacgcttatt aaatggagtg tatcaacggg atcttcctcg caaacgcaat gtcgtcagtg
      721 cagagaagta ttgtctggaa atctgggaaa acaaactttt caccagaaga atcctggagt
      781 ttgacagttc aaatggtgtt ctgtacgcgc tgaaacataa aagacactat cgccgggata
      841 agatgattgg aagggttgaa agtcggtgta tcaaagacat ttgcgaatac cagatgcagc
      901 tatccgggga gaagacgaaa tatgcctgtt ttatttatat cgaaagaaca atatacaatc
      961 acgataatcc atctgataaa atacctgtaa aagcggctgt tggtaatgca gtcatcctgc
     1021 tggcaaagga tgtgatatat aacgagtact tttttgattt gcggcagtca ttttttgtga
     1081 gcgttacaga tctgatggtc tctggcgcaa aaggtattcc ggagacacag acatatccgg
     1141 acgtttattg ctggatccct cttttttcca taaattccgg cgtgctcatt acccctgtat
     1201 ataaaattga ccctctgaaa cccgtaacgg taaaaaaacc ggataagatc actgttgttt
     1261 gtaattacag agaataattt cagccttcta ccttcaccgc gacagtgttt acctctgctt
     1321 tatcttccgt tcagtaaata caaaccaccg ctctggcagg tacagggcgt ggtttatctc
     1381 cgatactccc ctgtgcagta ataatcatca ctgaaaatac tgatcccgtc acggcaaaac
     1441 gccatcccat attccggcaa cacaaaatca tcttccgttt ataccggatt cactgacaaa
     1501 acagacaacc tgtattgcct gtacacaaca gtttctctcc tggcaggaat attcccctac
     1561 tcatttttta cgtttctccc cggttgttat gccggtgaag gtggtgcgtc gttttcatca
     1621 ccaacgccgg ttgagattaa caacatccgg aggaacattc tcatgaatac cccagtttca
     1681 ctgatggatg accagatggt cgacatgtcg tttatcactc agctgaccgg cctgaccgat
     1741 aagtggtttt ataaactcat caagcatgga gcctttccgc cccccatcaa actgggccgt
     1801 agctcccgct ggctgaaaag cgaagtggaa gcctggctgc aggcgcgtat tgcacagtcc
     1861 cgtccgtaat tcctgaccgt tatccgttcc accgcagcaa aggcatgatc cggcccccgg
     1921 aggcattttg ctgcgcgcta aaggagagaa tattatgcaa caacttgaaa acaggtgtga
     1981 cctgctgctc atccagcatc agaaatggat gacaagcgtg acgaggctta tcgtcgctca
     2041 tggaatgggg tctccgcatc ttcatggtta tcaccgtctg accctggcac acttttttct
     2101 gccggaaaaa ggctccgtca tttcggtggc cccccaggga ctctatcagg tcgtcaaccc
     2161 gggaacacca ccgttcattc ctgccattca ggaagggtta atgaccagca ttcagaccca
     2221 tgaaatcatg ctgcttacac attttaatct gggcggcgtt ttactcagtg agctacatcg
     2281 tctgggtgaa aaccggctgg caaacaggct gaacagcctg ctccggcgtt tcgacgatcg
     2341 tgatctctac cacacgctga tatggctgtg ctggtatgat ctgatgtgtg cccactccat
     2401 gcagccctgg actgaagagt taaagcataa atcacatgca gagcttgaaa actgggctgt
     2461 ggcgcgcaaa cgggaaaagc gcgagctgga actgatgatt gacgagtacc tgttatacgc
     2521 ctgctgattc ataacgttcc cggggttcct tcctcatcgt tacacacagc cggacggcta
     2581 tctatggctg tccgccaaat gaccatcgcc gcatttcgtt ccgctgaaaa cggtacgtag
     2641 tcagtgatac cggaatttcc ggagagggcc gcaggtatat tgcttctgtg caattgatgc
     2701 ttatttacat tggatgtagt tatttctgca tccaatgcta tcggaaagag attaaccagc
     2761 aaatggttcc tcaaatcgta ttgatattta cccggcgtat tgagcaccac catctgacac
     2821 tccgttcccg catcaaacga ctggaccgaa aaacgattta cttttcacgt gctgtggaaa
     2881 ttcacgaaaa aattattggg tcattcatcg aaaaacatat gttctgatga ctggagtcat
     2941 cctccagaaa tatacgtcaa ctgaacaata tcgatttttt atgggattct atacactttt
     3001 ccatatccct ctgaaaatca gtcatcagct caccaacacc tcttctgaaa cttgtattaa
     3061 acttattgct taaatagatg atataacatc tcttccattg cactgaatcg gcgaaaactg
     3121 ccgtttcaat aatctcaaca atattattta tcggctaact ttatcagtaa acaatggaat
     3181 gtcacactga tgaaatgaac attcagagta agatgataaa attcatataa acgccattgc
     3241 tgaatattat ttatttcata cagataaacg ataaaataca aaagggtata tttctcaatg
     3301 taacagtcaa ttattcatct ccactggcga gcacggtcag cacacaaaca aagttagtcc
     3361 tcctgcttaa tcattgcgta tcagaaacta aaaaagaaaa aattctgcag acaacttaac
     3421 aatcttcatc acatttcctg aagacagaca tatccacaaa cggtagtcag cgttgaatta
     3481 acaagtctta cagagctata cgtagcttat aaagccactc aatgttcatc atcacccgac
     3541 tacttatctg gaataccacc agtataaact catcaataaa aacacctgtt gttaaaataa
     3601 gaatactgtc agcagtaaat tattaaaaca acatctgtcc acactatctg ttcttttaac
     3661 gccaacggca gcgaaaatag tgcatcacag ccttcttttt tctttcgcca ccactcagac
     3721 aaacacaaca ctcacaccca caccacaaaa cagaatcaaa acataaaatt aacaataaca
     3781 gtcaaaagga aaacacagaa tattgaggtg gactgtcacc aggacagcgc atacgacgca
     3841 tgtgatcagc acatcccgtt cttcgctggc atccccaaaa tgcagaacaa acaatcataa
     3901 cattatgaat ataaaagaaa atatgatttt tcaccatgaa aaaaacatca atgtatctct
     3961 gtataaaaat aatgataaca tgacataaac atttaaaaac ataaataaac agcaacaaaa
     4021 acatttgata atcacttagt gattaaatac aattaagacg attttgttat aagagaggca
     4081 ccatggagtg agataatgaa taacgaacag atgcttatca aatgcagaga tccccgtaaa
     4141 tgtcggtttt atgccgaata tctgaatcac aaatacccta atgaattccc tcactttgcg
     4201 cctgacaacc cacaaaattg tatcaggaat gttaatttat ttttaaagga aaagtattgt
     4261 aatgtttcac ctcccccctt tggtactttt ggtactactg aagaagaagc actcaataac
     4321 gaaattaatg agcttgaaat tattgccggg caggcaatac tgcctgaaga gagttttctc
     4381 tggttgaaaa ctgacgaact tgcgactttc tttacctgga ctactattta tctgtcgaag
     4441 gaagaccata caaaattccc gaaaataaac cataattcat caggaataaa aaacggaatt
     4501 gaagtcagga aaatacgaac agttaccgat gaaacgttaa acacttacaa aaaaagtaac
     4561 cttccggata atccatcatc ccataacgaa cgaataaaag taatcatttc atattttgac
     4621 tcctgccatt ctttaattaa taaaaatgca aaaagcatat acctcgagga actcaggcag
     4681 gcgtggctta aaaagaaaag cgcttgtcaa aagtttaagt ggctccttcc tgacgatgat
     4741 gaaatgtgtc agtggttctg gacaagactg aaacaaaaac aggatgaaaa acatccagta
     4801 cataacccag tgacccggtg gttcatacca tcttcaacga aagaatatta tcttgcctct
     4861 cttgtcgcct ttgctttatg gaaagaagcg cctgatacca ttgaactgtt cagaacacga
     4921 ataaatcatg cctggcatca gaaaaaacaa aaggacaaat atcggagtga tggtaaaaag
     4981 gccattaata tacatataag aagcaatgtg aaagaaatgc ttgatgagct cagaactgtt
     5041 tacggtctga gaacaggaga gttttttgaa atgctgatta cagagaaata tcttgaacac
     5101 aagcgaagga agtaattcat tacctgttac attctaaata atctcagaca gatattatct
     5161 ccgtgaatcc cccgccacct ttccggtgcg
//
LOCUS       DAJVPT010000186         5186 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVPT010000186 DAJVPT010000000
VERSION     DAJVPT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(168..329)
                     /locus_tag="OQM47_003821"
     CDS             complement(168..329)
                     /locus_tag="OQM47_003821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481785.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(326..514)
                     /locus_tag="OQM47_003822"
     CDS             complement(326..514)
                     /locus_tag="OQM47_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1481786.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            774..1109
                     /gene="iraM"
                     /locus_tag="OQM47_003823"
     CDS             774..1109
                     /gene="iraM"
                     /locus_tag="OQM47_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1481787.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1390..1498)
                     /locus_tag="OQM47_003824"
                     /pseudo
     CDS             complement(1390..1498)
                     /locus_tag="OQM47_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2021..2097)
                     /locus_tag="OQM47_003825"
     tRNA            complement(2021..2097)
                     /locus_tag="OQM47_003825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2061..2063),aa:Arg,seq:tct)
     gene            complement(2199..2274)
                     /locus_tag="OQM47_003826"
     tRNA            complement(2199..2274)
                     /locus_tag="OQM47_003826"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2239..2241),aa:Ile,seq:cat)
     gene            complement(2419..3240)
                     /locus_tag="OQM47_003827"
     CDS             complement(2419..3240)
                     /locus_tag="OQM47_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1481788.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3255..3611)
                     /locus_tag="OQM47_003828"
     CDS             complement(3255..3611)
                     /locus_tag="OQM47_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1481789.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3624..4673)
                     /locus_tag="OQM47_003829"
     CDS             complement(3624..4673)
                     /locus_tag="OQM47_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1481790.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4675..4953)
                     /locus_tag="OQM47_003830"
     CDS             complement(4675..4953)
                     /locus_tag="OQM47_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481791.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            4894..5079
                     /locus_tag="OQM47_003831"
     CDS             4894..5079
                     /locus_tag="OQM47_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481792.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(5121..>5186)
                     /gene="hokD"
                     /locus_tag="OQM47_003832"
     CDS             complement(5121..>5186)
                     /gene="hokD"
                     /locus_tag="OQM47_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1481793.1"
                     /translation="EVRIRTGQTEVAVFTAYEPEE"
BASE COUNT         1379 a         1398 c         1108 g         1301 t
ORIGIN      
        1 tttttccatc tggtccatac cccgtctccc gttatccgga agctgacaac aataaaaaag
       61 ccaccagtta actactgatg gctctgataa ctcatgcaag cgtctcagac gacccactga
      121 cactaccggt gagtttaacg ataccttcca tttgactggc tcacttttta tgatgatgcc
      181 ggtgcattta tctccagcac cagactttct atctcaacgc catacgttgc atttttggta
      241 atatccgtca gcgtcagtgc atttagtccc actgccagac tgtcttttat ggcctggaat
      301 gccgggccag tacgatgacg tagtatcact ccggctcagt tgcaccactg accaccacat
      361 caccttctgc tgcaatcgcc tgcatcaggg tataaggggt tatggccacc ggactaccaa
      421 acggctgcca gccctctttc agtttatgtg tcagcttttc cgcaagatct gacggcggcg
      481 ccgccctgac aacatcatag tgtttaaatg ccatggttct ttccaacatc tgaaaaataa
      541 ttctttaaaa tacctgacat gtaatacaga aaaaacacaa aaccatacct taaataaaaa
      601 cctgattatc aagcagatat gcatggataa actacaagac gagatataaa ccaccctgca
      661 tttaaataaa caataaacaa catcagaaaa ataattctgt tctatggttt aattcaaaaa
      721 tatcatttat acttttcaga acatcaccag caaggcataa acaaggaaac taaatgaagt
      781 ggattgtgat tgatacagtt atccagccat catgcggaat atctttttca gtcatatgga
      841 gtaaaataaa attaataatc tggtatcaat cggatgcttt cttacctcct gaaagtatat
      901 ttacactgac tcacacaggc atcatgctca ataacaaagt gctacctgta accatttaca
      961 acgtagtacc attcaataaa acattctgga atttaatcaa aaacagccag gaatgcccta
     1021 caaatacaga taacgtattg aatgaatgct ttaataaccg ttgcactctg caaatatgcc
     1081 cttatggact aaaacaacaa agtccataag gagtttactc acatctgaca aaatcaatat
     1141 aaacagcccc tccggagagg ggctggagag tggcgctatg tgccattcca tggtgccggg
     1201 tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata tacctactcg
     1261 ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca aactccccgc
     1321 aaaccagaca acagtcaacc gcctgaattg tgaagtattt aaaaatttct cccgctaact
     1381 gatacccggc taacagtctg gcgttttctt tttcagcaac gggaaagcaa caaccaccac
     1441 acccgccacc agcacaccgt cagccggcac tgacattatc cggctgctgc aatgccatta
     1501 acaaaaacag taagcaatca ctttttaccg taacaggtga taatccagat atgtatctac
     1561 cccagatgag taatccgaag ttcatccata ccacaggtcc tggctattct gttgtactcc
     1621 tgaacaagag caaataattc tgaattagca accatgaact catcgcaaac cctctgtata
     1681 gcatcactat tcagaataat aacgtctctt cccgaaagac gatcaggagt acagaacaaa
     1741 actgtcaaac ggctgaaggc ctttgctcgt gctgcattga ctatatcaat acgctgccta
     1801 aggatgaaac accccgacgc ctcatcaata ttcactctac ccacaccata tgaatgataa
     1861 atacttaatg ctgaaaaaac cattagaccg tataacaaat actcaatcaa tacttaacag
     1921 aacttttatt tttgacaaat atataatatt ttcaacaata tcctgagcca ggtatatttc
     1981 agtataaggc tctgccggaa ggaatctgga agaatgaata tggcgcgctg tactggattc
     2041 gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgctga
     2101 ataccgataa tggaccgcca tcggggaccc gccccccgca ccaacaaccc tgttatcgtg
     2161 tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg ctggatttga
     2221 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggagc
     2281 agaataataa cggtccgtaa ttaatttcgc aataaaaaaa cccgctcagc ggcgggttgt
     2341 agaaactctt ctaacgccag gcataaaacg cccatcatta tgacgaattt accacagatt
     2401 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     2461 ttcaatatca aaccgggtca ccagcgcatc ataaaatttc ttaactgttt tttcccatga
     2521 cgcgcgtgtt atctggtttg tcacctcgca tatagcatta aatgcctccg ttgatggtag
     2581 tctttcatag ccacgaccac cacaacgctg gcagtctctg ataacaggca taccacgttt
     2641 taccgactct tcacgatgaa tggcgacacc acgcccacgg caatccttac aggcggtgga
     2701 aacctcaccc tttccgccac actccggaca ggcaactttt accacctccc tgactttttt
     2761 ccattcttcc cagtaaaacg gatacacacc tttcgtacac tttgcccata ccggcggctt
     2821 accatccgga tactggatct tgtttgtaaa aactatgctt tcaataaatt tttccccata
     2881 gcaacaaggg cactgctttt tactcgctgc gctgcgggca taatcctcaa aagcgtacga
     2941 agccataatg cgcatcacta ccggttttat ttctgccgga agttttctca acgccgccac
     3001 acgatcgcac cgactgagtg cataatctgc cagtaattct gttgcccgcg ccctgtcatt
     3061 catactgatg cccattttcc ccaggaacgc agaaaaaccc atctcagccc gattctgtgt
     3121 catgccctgc gcggccatca catcagtgat actcagcgca tcttttgacg ttgaggccga
     3181 tgcatcggtc aggccagggg attttgggga gtagtatttc ggtaaatctt ccagtttcat
     3241 tttttgacct gctcttaatg cattatgggg taaatcttca cccccagacg tccaccagat
     3301 actggctgac cacgaacgat attgatttca tcaaactgct catcgtccat taacactccc
     3361 gcatgcgtca gcgcatccag cggtgctttc aggatattgt ccaggtcgcg acgacgctta
     3421 tccggtggct ctgcaatcac ctttatcgcc agccttccgg acaggcttaa tttcagccgc
     3481 tgctggcgaa caataagcgc cacagcccgg cgataacgct ttccctcctc cgagataaaa
     3541 tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg gcgggtaagg taaaaccaaa
     3601 tctatgagca tcagtcacct cttttaccca agcacgccag ttgcaaaggc gtgatcaaga
     3661 aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt cgaacgccag aggatccgca
     3721 tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga aaatatcgtg agatttggtc
     3781 cccattccgc cctgaccatg accaatcagg tgatggggat cgtcggctgg cttaccacaa
     3841 cacgcacacg gctgtgtctt cacccagcgt ttgtatttct cgttaaccca gcggcgacgt
     3901 ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa cggcaatgct gaccaccgtc
     3961 ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca gcggcgcaag attttttgtg
     4021 cgctgcttca gtatgctggt ggcggtctgc tctcccggca cgatgtcgct ttcacggtac
     4081 attgagcgga ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc
     4141 gcgtccgcca cctgattgcg gaccgcccac caggataatt cagccagaga taattcacgc
     4201 tcctgcgtac cgcttattgc gtgaccgatg acgtcaatca tccatgctga caggttttga
     4261 tgagcaagtt gctcgagtga ttcggatgtc tggtcacgca gctggttgtc gcagtgccag
     4321 cacaatacca ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg
     4381 ccgtgtggcc actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcgccagca
     4441 ccaccagcag cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc
     4501 actagcggct ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt
     4561 tccggctcca ccagtacccg ggtattgtgg aataccggca tggattcacg accgggctta
     4621 aggaccacca gcccgagttc cggaaccaga acaggtcgaa gtaatacccg cacattacct
     4681 ccagatccgt tgctggaatg tgcaggacgg acgcggtggc cgttcggaat aagggagcct
     4741 gacgaagatt atccagtgac gataatcgag actgagggct ttcttaatct cgtatccgtg
     4801 tctgcggtag cgctgaatca gccattcagc ctgttcttcg gtgcaggggt cgtgctgata
     4861 cccgtcagat ttgaatgcat gagaacgccg cccgtgcctg ctggcagggg cggcagagtt
     4921 atccgaattg taaaatttgg tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg
     4981 ccagttgttc aggctgacgg atggattgta aaccagaacg accagaaaaa acaaaacccg
     5041 ccgaagcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagcgg tggcgaggga
     5101 tttctccccc gccgggtctc ttactcctca ggttcgtaag ctgtgaagac agcgacctcc
     5161 gtctggccgg ttcggattcg tacctc
//
LOCUS       DAJVPT010000187         5165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVPT010000187 DAJVPT010000000
VERSION     DAJVPT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1320)
                     /locus_tag="OQM47_003833"
     CDS             complement(<1..1320)
                     /locus_tag="OQM47_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1481794.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDY"
     gene            complement(1336..2220)
                     /locus_tag="OQM47_003834"
     CDS             complement(1336..2220)
                     /locus_tag="OQM47_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1481795.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2231..2836)
                     /locus_tag="OQM47_003835"
     CDS             complement(2231..2836)
                     /locus_tag="OQM47_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1481796.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3007..>5165)
                     /locus_tag="OQM47_003836"
     CDS             complement(3007..>5165)
                     /locus_tag="OQM47_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCS1481797.1"
                     /translation="EQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGN
                     LTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIK
                     GSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLIND
                     EDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIA
                     TFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPV
                     VLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYK
                     DAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMK
                     HHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYF
                     CCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKA
                     DSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLK
                     ELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWS
                     TLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLL
                     ALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1514 a         1269 c         1064 g         1318 t
ORIGIN      
        1 gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat ccagtacata
       61 gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg cgtggagatc
      121 ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa taccgcgcca
      181 tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac tgccttgcgg
      241 ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac tcattccgag
      301 atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa agcgatacgc
      361 taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc gatccgctga
      421 tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag catataagcg
      481 gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac ccaatgtgtc
      541 gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc tctgataaaa
      601 atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa gttgcgtggg
      661 gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga gatcgagcaa
      721 gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg ccacccgttg
      781 ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata gaccacgcag
      841 acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa ccttaaaggc
      901 cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc atgtggcaac
      961 aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga cctggctgtc
     1021 gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa gagacgggct
     1081 ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat aaaaagcagt
     1141 atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt catgcccgcg
     1201 atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt gccgataata
     1261 caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg aattttccat
     1321 tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata ccggagcggt
     1381 gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata gttttccact
     1441 atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc aaaaaccact
     1501 aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg tcagcaacgg
     1561 tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac cttccagcgt
     1621 ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc cccataaata
     1681 ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta acgccccgcc
     1741 ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg caaaaacggt
     1801 cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg ggtcacccac
     1861 aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata acagaaacag
     1921 ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat ataaccaacg
     1981 cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac ttatcagcgt
     2041 aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac aaatgacgat
     2101 gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag gtaaccgcca
     2161 tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt tgccagagat
     2221 ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga ttaatcgcag
     2281 tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc atccactgtg
     2341 gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca cttttgccaa
     2401 atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg caaaactcgg
     2461 tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc ggtaaaaata
     2521 aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc aaaatcgccc
     2581 ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca ttgagcgcca
     2641 tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc accaacacca
     2701 acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga ttcgctgtaa
     2761 cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag agcggtttta
     2821 tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt gcatcaattc
     2881 ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata tcaaggtatt
     2941 gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa attattacgc
     3001 tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg cgcgcgtctt
     3061 accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct ctaacgccag
     3121 caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg gattattcat
     3181 cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg gggtgtggcg
     3241 gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg tactccacca
     3301 tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg ggtcgagtgc
     3361 ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct gcctcagaaa
     3421 aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga gaacaacagc
     3481 gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt ccaaacgtat
     3541 cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa tcagctcaga
     3601 caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat tatttttccg
     3661 atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat actgattttt
     3721 cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt gtaaagcagc
     3781 gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt ttttctccat
     3841 ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca cacgtattag
     3901 atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac tctcttgtgg
     3961 cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg ctaagcttgt
     4021 gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag tgagttgtcc
     4081 tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat caataatgga
     4141 ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc aatctctttt
     4201 cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat tcaacggacc
     4261 gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg gttcacggtc
     4321 gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact ccagaatctc
     4381 attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc tattaataag
     4441 ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca ttatggcaag
     4501 atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat ccagcatttg
     4561 caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt ttttcatctg
     4621 attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt tcagagcatc
     4681 ttcatcattt attagacccc aataatatgc atcaggattt attatcatca gcgctttttt
     4741 gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat ttgtcgaaga
     4801 cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa tactcccctt
     4861 aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg catttatcga
     4921 accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt taccgagaat
     4981 aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca aattgccaag
     5041 aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc cacgatgcac
     5101 tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt tgatagcttg
     5161 ttcaa
//
LOCUS       DAJVPT010000188         5114 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVPT010000188 DAJVPT010000000
VERSION     DAJVPT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1761)
                     /gene="xdhA"
                     /locus_tag="OQM47_003837"
     CDS             complement(<1..1761)
                     /gene="xdhA"
                     /locus_tag="OQM47_003837"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1481798.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLK"
     gene            2176..2931
                     /locus_tag="OQM47_003838"
     CDS             2176..2931
                     /locus_tag="OQM47_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1481799.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3010..3083
                     /locus_tag="OQM47_003839"
     tRNA            3010..3083
                     /locus_tag="OQM47_003839"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3042..3044,aa:Gly,seq:ccc)
     gene            3218..4828
                     /locus_tag="OQM47_003840"
     CDS             3218..4828
                     /locus_tag="OQM47_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1481800.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            4860..>5114
                     /locus_tag="OQM47_003841"
     CDS             4860..>5114
                     /locus_tag="OQM47_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481801.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKL"
BASE COUNT         1337 a         1237 c         1309 g         1231 t
ORIGIN      
        1 ttttaacgac attaacggct cttccggtcg ttcaaccagc acgatatggc cttttatcag
       61 ggtcaaattc atcgctgact gatgtagcat gactgcggcg tgagcgatga ttttctcttt
      121 taataatagt gccgcactgc gcagcgcagg cgcggcaaca tagctctggc gtgaggcaaa
      181 tgcgccggga tcgaacggcg taacgtcggt atcttgtgtt gaaataacgc gaacgtcgct
      241 gaccggaacc cccacggttt ctgccaccat ttgcgagaag acggtgtcgg caccctgacc
      301 gatttccgtc gcgccgcttt gcacgttgat ggttccatcc tgattcatca gaaggcgcgc
      361 gcctgctatt tctacgccga caggccaggt gttagaggtg tagctaaaac aggcgacgcc
      421 aacgccgcgg cgcaaattgc cttgctggtt ctggcattct gcacggcgtt tttcccattc
      481 aaagattttc cggccttttt caagacactc cggcaaccct gcgctgcaaa tacgtttgcc
      541 cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt aaacgaattt caacaggatc
      601 aatacctaac gctgtcgcgg cgtcatcaag catagactca acggcaaata cgacttgtgg
      661 cgcgccataa ccacgcatcg caccagccga ggggaggttg gtatagcagg tctttgaact
      721 gtaagcgtag gcacaacgag gataaaggta agcgacttta ttcccccccg cagaagcgat
      781 ggagtgcccg tgagatgcat aagcgccggt gttagacaga acatccagac tataaccttt
      841 caaggttccg tcgcggttca cgcccatttg cccgtcaata gtaaaagcgt ggcgggtacg
      901 ggttgcgagg aaacactctt cacggctaag ggaaactttc accggaatgc cgccaagctt
      961 gctggtcagg aatgccgcca ttggctcttc cagtacatcc tgtttattac caaaaccgcc
     1021 accgacaaac ggtttgatga ctcgtacgca tgaccaggga atatccagcg cctgaccaac
     1081 cacgcggcga acaatgtgcg ggatctgggt gctggaaacg atggtaattc gcgagtcatc
     1141 ctccatccac gccagcgatg ttacgctttc catgtgacaa tgttgaataa cgggagtctg
     1201 atagtgcccc tgtacctggt agtcggcggc atcgattgtt tgttggacat tgcccgtcga
     1261 catcgtgctt tgtttcagta aattgccgcc gttatggatt ggtgcagcgt cttctgtcag
     1321 cgccgcttct ggcgtggtga taacgggtaa ttcttgccac tcaatgctga ccaattgcgc
     1381 cgctttttct gccgtgagtt catcgcgggc cacgacgatg gcaacggcgt caccatgatg
     1441 acgaacatgg cgagttagca gtgcgcgatc ggcggtatcg cgcttgtttt cgtcaagtgt
     1501 ccaggcatgc ccagctgtag cgaatggaat atcaggcaca tcttcccaag taaaaatcgc
     1561 cagtacgccc ggtaaacttc tggcttgttc atcattaata cttacggcat aaccatgtgc
     1621 gatagggcta cgtacatatt tcgcgtaaca catgcccgcc ataacataat cgtcagtata
     1681 tcgtgcccgc ccggtgacct tagcaatggc atcgacgcgc atgcatgatt cacccgtagc
     1741 ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt cttatggcaa
     1801 agatttagtc taagcaggca agatttacgc cagaaacgac gataagcgtt aggggaggaa
     1861 ggaagtgtga tgacgttaaa aaaacacaga tatgatacga aacaggactc tgaataggga
     1921 cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaatcag gattattacg
     1981 catcaggatg ggaaataggg taaaaagtca ggaacttcga tacctctaca gcgaatattt
     2041 tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta ccacgttttt
     2101 ccagaagcaa gagattgggt atcaagaggc tggctgctat gatatggcgt cttgtttttt
     2161 aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg tatcgtgatg
     2221 tttttatcgg ttggactgct attggcgggc tgttcgggta gcaaatcatc cgatacagga
     2281 acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg tatttcgcgc
     2341 accacgggaa ccagcgtaaa agagctggcg cgactgaacg gcatttcccc cccttacacc
     2401 attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag tacacgtaaa
     2461 tcaaccgcca aatcaacgac taaaaccgca tcggttacac cgtcatcagc ggtaccgaaa
     2521 tcttcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg gaaagttatc
     2581 atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc tccacggggt
     2641 acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca gctgcgtggc
     2701 tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta cgcccataat
     2761 gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat cgccactatg
     2821 gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg tgcaacggca
     2881 attgatccgc tacgttactt gccgccgcag ggcagcaagc caaaatgctg atggcgaatt
     2941 aatcagcagt cagcagcgcg gcacttgcta aggagagcgt aaggtttata atgccttacg
     3001 catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc tatacgaggg
     3061 ttcgattccc ttcgcccgct ccatgacact tcttaaagtc tactaactta ctgaatccta
     3121 agggatacgg cttaacgcct tccgatagtt ttactcactg taaccaacac tgtgactaac
     3181 tttgttgaag tagtgagcag acctaaggtc tcagtacatg ctcaagtcaa gaacctacct
     3241 ttatcagcgt aatggtgtct tttacatccg tcttaggatg aagaccacta gtcgcctgac
     3301 cgcttcgctt ccttctcaca accgttataa actagcgtca gtatctttac ggaccaagga
     3361 tagacgtacc gctatggctc actcacggca catcaagtca gcacttaaag caatccatgc
     3421 agacaaccct aacgcctctt atgaggagct acgggagcac ctgaagacca ttgtcgagtg
     3481 ggaacttagt gtcagccgtg atgacctgaa cgacccggag tcctaccagc tttacgttga
     3541 ccagtacgat gacattaagt ccaaccttcg ggaagctgta gcgaccgagc gtctgactgt
     3601 agaccagcat cgctatatta atgacgttat cggtgtgctt aaggcctgtc aggatagact
     3661 caatggcgat agctctggcc tattgtctta ccttgagcct gagactggca gtctaagacc
     3721 gtccgtctcc ctatctgtat tggcggaacc tgaagtccct gaacctaaag cactgaccct
     3781 cgcgtctctc attgagcagt acgagcagga gaacgcccag aactggaagc cagcgaccct
     3841 aagcgaaaac cgagcgtcac actctacgct aatcgagata ttcgactatc tggatattca
     3901 ggatgtcggc aaggcgaccc gtgcagatat gctcagagtt cgtgaagtcc tccagcagct
     3961 accgaagaac cgcaagcaac gctttaagtc tatgccgctg tctgacttgc tgaaccggga
     4021 gtctaagact gactgtctgg acgtcgttac cattaacaat aaatatctta tcaagatggc
     4081 tgctgtgttt aagtgggcgg tacgcaacga cttgatagct aagaacctga ccgaaggtct
     4141 tgagctgaaa gtccctcagc gtaaagcctc cgacgctcgt gatgcgttca gtcctgagca
     4201 ggtagggcaa ctactggtcg cagctaaagc gtactcgcag aagacctcag gtaagccata
     4261 ccattattac gtcaccgctc tggctgcgat tacaggtgcg agacttaacg aggtggccca
     4321 acttcaggtt aaagatgtca ggaccactga ggctgggacc gtttttatcc atatcaacga
     4381 agacgatagc agcctgccgg gtaagagtat caagaacgcc cacagtgacc gctgtgtccc
     4441 gttggtagat ggtgcttatg gttttgtatt ggctgacttt atgtcactcg tggaagaccg
     4501 tagaaagact gagggtgata atgctatggt gttcaatggc ctcaagctaa tgaagaacgg
     4561 ctacggtgag caggttagca agtggtttaa ccgaacgttg ctgcccaaag tcctcgctga
     4621 ccgtagcggc ttagcttttc actcgtttag acatacagtt gcgacccagt taaaacaaca
     4681 tggggtcgag ttagcgtatg ctcaggcgat tatgggtcat agctcagggt ctataactta
     4741 cgaccgttac gcgaaagagg tcgaggtgga aacgctgaaa gaaaaattag cggagtcatt
     4801 gtcagtaaaa aaaatagacg gaaagtaatc gcatagggat agcggaatgt gaggacgtaa
     4861 tggtcaaaga atttttagtt agcaaagaaa ataagccttc atatgaatgt atgaatactg
     4921 ctgaaacttg tttggggctg gctggaatgg cttcggaaga ttggcaacgt gagcatcatt
     4981 gccgcacagg tataatattt actgcttttg ctattgaggc aatgttcatc ttctatagaa
     5041 aacaggttga ccccggctac gataagacgc agaaagagtg cagaaagaca atgcacaaga
     5101 atacgttaaa attg
//
LOCUS       DAJVPT010000189         5060 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVPT010000189 DAJVPT010000000
VERSION     DAJVPT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..72)
                     /gene="hokD"
                     /locus_tag="OQM47_003842"
     CDS             complement(<1..72)
                     /gene="hokD"
                     /locus_tag="OQM47_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1481802.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALV"
     gene            complement(724..1140)
                     /locus_tag="OQM47_003843"
     CDS             complement(724..1140)
                     /locus_tag="OQM47_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481803.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1167..1307
                     /locus_tag="OQM47_003844"
                     /pseudo
     CDS             1167..1307
                     /locus_tag="OQM47_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1307..2332
                     /locus_tag="OQM47_003845"
     CDS             1307..2332
                     /locus_tag="OQM47_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1481804.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2385..2474)
                     /locus_tag="OQM47_003846"
     tRNA            complement(2385..2474)
                     /locus_tag="OQM47_003846"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2438..2440),aa:Ser,seq:cga)
     gene            2568..3365
                     /gene="mtfA"
                     /locus_tag="OQM47_003847"
     CDS             2568..3365
                     /gene="mtfA"
                     /locus_tag="OQM47_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1481805.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3466..3541
                     /locus_tag="OQM47_003848"
     tRNA            3466..3541
                     /locus_tag="OQM47_003848"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3499..3501,aa:Asn,seq:gtt)
     gene            3703..4965
                     /locus_tag="OQM47_003849"
                     /pseudo
     CDS             3703..4965
                     /locus_tag="OQM47_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1400 a         1192 c         1133 g         1335 t
ORIGIN      
        1 taccagtgcc gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt
       61 ttgctgcttc atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt
      121 gttcagcatg gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa
      181 ctggggcttt tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac
      241 ccgcagtcat tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa
      301 tatccaacta aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg
      361 ccatgaacac ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa
      421 ttttttccat ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg
      481 caacaaacaa aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac
      541 atttaaacac acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta
      601 catagcgtta caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca
      661 gtccgttccg cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct
      721 ttttcagttg gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc
      781 tggtggattt tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga
      841 tgtaaccggg actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt
      901 acaatcctct tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc
      961 cataacacct tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc
     1021 ccgtgcaatt ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt
     1081 cgtaatcgct atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat
     1141 tttcattacc gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc
     1201 cgttctatac caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata
     1261 aaactttacc accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag
     1321 gaaaaatcct gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt
     1381 atggaaacca acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc
     1441 tttatggaaa aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg
     1501 cttgtgggaa aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa
     1561 agattatctg caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc
     1621 cataaaacca gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca
     1681 agccaaccat gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta
     1741 cgtgaaggct aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata
     1801 tgtaaccgac aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc
     1861 aatggaaatt gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga
     1921 gcagattgga aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa
     1981 agcatggagt ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc
     2041 ctatgtaatc agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg
     2101 ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca
     2161 tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt
     2221 ctctggtcac aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc
     2281 gacacttgat gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt
     2341 gtgatttttg tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg
     2401 atttgaaccc ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc
     2461 tccggcatct ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt
     2521 ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc
     2581 cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga
     2641 tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac
     2701 gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa
     2761 gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg
     2821 atggttttca tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag
     2881 acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag
     2941 ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc
     3001 tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag
     3061 ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga
     3121 acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg
     3181 ctgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag
     3241 aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag
     3301 atcctttgca gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc
     3361 attaattaac aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt
     3421 gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag
     3481 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     3541 aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt
     3601 gtgaaaactc ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc
     3661 aaatctggga tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca
     3721 aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg
     3781 tatctgctgg tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt
     3841 aaagaatccc gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag
     3901 caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct
     3961 gctgaacgtg gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat
     4021 aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat
     4081 cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt
     4141 gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag
     4201 cacctgagta acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca
     4261 gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg
     4321 gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc
     4381 cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc
     4441 cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc
     4501 attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc
     4561 ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa
     4621 ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag
     4681 gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca
     4741 atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag
     4801 atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg
     4861 gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct
     4921 tatgcaccgc cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc
     4981 cacactctgg caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa
     5041 cttcatgacg aaaaacacct
//
LOCUS       DAJVPT010000190         5026 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVPT010000190 DAJVPT010000000
VERSION     DAJVPT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OQM47_003850"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OQM47_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1481806.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OQM47_003851"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OQM47_003851"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1481807.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OQM47_003852"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OQM47_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1481808.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OQM47_003853"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OQM47_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1481809.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5026
                     /gene="yidZ"
                     /locus_tag="OQM47_003854"
     CDS             4379..>5026
                     /gene="yidZ"
                     /locus_tag="OQM47_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1481810.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTW"
BASE COUNT         1144 a         1208 c         1257 g         1417 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acctgg
//
LOCUS       DAJVPT010000191         4687 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVPT010000191 DAJVPT010000000
VERSION     DAJVPT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(25..762)
                     /gene="bamD"
                     /locus_tag="OQM47_003855"
     CDS             complement(25..762)
                     /gene="bamD"
                     /locus_tag="OQM47_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1481811.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            897..1877
                     /gene="rluD"
                     /locus_tag="OQM47_003856"
     CDS             897..1877
                     /gene="rluD"
                     /locus_tag="OQM47_003856"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1481812.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1874..2605
                     /gene="pgeF"
                     /locus_tag="OQM47_003857"
     CDS             1874..2605
                     /gene="pgeF"
                     /locus_tag="OQM47_003857"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1481813.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2735..>4687
                     /gene="clpB"
                     /locus_tag="OQM47_003858"
     CDS             2735..>4687
                     /gene="clpB"
                     /locus_tag="OQM47_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1481814.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPP"
BASE COUNT         1153 a         1147 c         1273 g         1114 t
ORIGIN      
        1 gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga tgattttcgc
       61 tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca tcagcggcag
      121 cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta tgcggttaac
      181 gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt catatttcgc
      241 cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact gactgttcgg
      301 atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt gaggatcgcg
      361 atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg ccatattggt
      421 caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa ggcgaataaa
      481 acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg cgtagatgag
      541 atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta acgcttccag
      601 ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag tcgcgtaaat
      661 ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg ccaaaaacag
      721 gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt gactttcctc
      781 aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct acaatagcac
      841 actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca gtatatatgg
      901 cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa cgcttagatc
      961 aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa tggatcctcg
     1021 accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa gtattgggtg
     1081 gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa ccgcaggata
     1141 tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa ccgcgcgacc
     1201 tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg ttgcttcatt
     1261 actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg gataaagaca
     1321 ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta gtcgaatctt
     1381 tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat atgaccgctg
     1441 gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac atggcggtgc
     1501 atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc cgtgtgcaca
     1561 cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg catatggccc
     1621 atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt ccgccaaaag
     1681 gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg cttcatgcaa
     1741 ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat gcgcctattc
     1801 cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag cataaggatg
     1861 aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa aaggtgttgc
     1921 ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact cactcaacct
     1981 cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc gactttttgc
     2041 tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca aagatgtgct
     2101 taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata gcaatacgcc
     2161 tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt gcaatcgagc
     2221 gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag gcgtgctgga
     2281 agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt tagggccggc
     2341 aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta tggcagtaga
     2401 cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg atatttatca
     2461 gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg gcgaccgttg
     2521 tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca ccggtcgtat
     2581 ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta cgcgtgattt
     2641 tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct catttaatct
     2701 ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc ttactaataa
     2761 attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca accaattcat
     2821 cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg ttagtccttt
     2881 attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc aggcattaaa
     2941 tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg atctggtgcg
     3001 cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta tctcgtcaga
     3061 actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc tgaaagcaac
     3121 aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag gtgaaagcgt
     3181 gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca tcgaccttac
     3241 cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag aaattcgccg
     3301 taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg gtgaacccgg
     3361 cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg gcgaagtgcc
     3421 ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg tggctggggc
     3481 gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc ttgccaaaca
     3541 ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg cgggtaaagc
     3601 cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg gtgaattgca
     3661 ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag atgctgcgct
     3721 ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata ccattgcgat
     3781 tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta ctgacccggc
     3841 aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc tgccggataa
     3901 agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg actcaaaacc
     3961 agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac aacaggcgtt
     4021 aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg aagaactgag
     4081 cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga aggcatcgct
     4141 ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta ttgaacaggc
     4201 tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa tcccggaact
     4261 ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc tgttgcgtaa
     4321 taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga ttccggtttc
     4381 tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac tgcaccatcg
     4441 cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc gtagccgtgc
     4501 ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc caacgggtgt
     4561 ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg acgaggcgat
     4621 ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc tggttggtgc
     4681 gcctccg
//
LOCUS       DAJVPT010000192         4672 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVPT010000192 DAJVPT010000000
VERSION     DAJVPT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..488
                     /locus_tag="OQM47_003859"
     CDS             <1..488
                     /locus_tag="OQM47_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1481815.1"
                     /translation="KAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMPTGGCTV
                     DSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASG
                     VGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIY
                     E"
     gene            822..3101
                     /gene="ydeP"
                     /locus_tag="OQM47_003860"
     CDS             822..3101
                     /gene="ydeP"
                     /locus_tag="OQM47_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1481816.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3349..3552
                     /gene="safA"
                     /locus_tag="OQM47_003861"
     CDS             3349..3552
                     /gene="safA"
                     /locus_tag="OQM47_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1481817.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3620..4381
                     /gene="ydeO"
                     /locus_tag="OQM47_003862"
     CDS             3620..4381
                     /gene="ydeO"
                     /locus_tag="OQM47_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1481818.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1335 a          995 c         1053 g         1289 t
ORIGIN      
        1 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
       61 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      121 gcggctgcac cgttgatagt cgtaatgtca ccgtggatct cccggacttt cctggaagcg
      181 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      241 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
      301 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
      361 tctccctggg gacagtaaat aaaagcaaag tacctctggg tttaagcgca acctatggac
      421 aaacaggaaa caaggtttca gccggaaccg tccagtcagt cattggtgtc acttttatct
      481 atgaataatt gtgattttga ccttagtcta tgcgctccat tcacgaaaat tgcgcggata
      541 attattttgt gaaggctatt atcctacacc tgtaagaaaa tccgcgcaca attgaagcca
      601 gagtcttatt ttttatattg gaaaatatgt taatttaaac actatgagag atgatgctta
      661 acttatcgta tttaatctat tgtttaacga taatagatta aaaatagaaa gagagtagtc
      721 ccttactctt atatatccat gttggcgata atccctttgt atgtactgtg catcatcgct
      781 attacaaatc ctaataattc atttccacac aggataagta gatgaagaaa aaaattgaat
      841 cctaccaggg tgctgcaggt ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta
      901 agcagatgga tatacgccat gatgttattg ccatgtttga catgaataag ccagagggct
      961 ttgactgtcc gggttgtgca tggccagatc ctaagaacag tgcgtcattc gacatttgtg
     1021 aaaacggcgc aaaagcaatc gcttgggaag tcacggataa gcaggtaaat gcctctttct
     1081 ttgctgagaa tacggttcaa tcattactta cctggggaga tcacgagctt gaggctgcgg
     1141 ggcgtctcac tcagcctttg aaatatgatg ccgtcagcga ctgttacaag ccattaagct
     1201 ggcaacaagc tttcgacgaa attggcgcac gccttcaaag ctatagtgat cccaatcagg
     1261 ttgaattcta tacttcgggc cgcacttcca atgaagctgc ctttctttat cagctttttg
     1321 cccgtgaata cgggagcaat aactttcccg actgctccaa catgtgccat gaaccgacaa
     1381 gcgtgggttt ggcagcgagt atcggtgtag gtaaagggac cgtgttgctg gaagactttg
     1441 agaagtgcga tttagtcatt tgcattgggc ataaccctgg tacaaaccac cctcgcatgc
     1501 tgacttcgtt gcgcgcttta gtgaaacggg gagcgaaaat gatcgccatc aatcctctac
     1561 aggaacgtgg cctggagcga tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact
     1621 ctgagactca gttggccagt gcctactaca acgtgcgcat tggtggcgat atggcgttgc
     1681 tcaaggggat gatgcgcctg ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc
     1741 cctcattgct tgatgacgaa tttattcaaa cgcataccgt cggctttgac gagctacgcc
     1801 gtgacgttct caattccgag tggaaagata tcgaacgtat ttctggacta agtcagacac
     1861 aaatcgccga actggctgac gcatatgccg ctgccgaacg caccattatc tgttacggaa
     1921 tggggatcac tcagcacgaa catggtaccc agaacgtgca gcaactggtc aatttgctgt
     1981 tgatgaaagg taacattggc aagcctggtg cgggtatctg cccactacgt ggtcactcta
     2041 atgtacaggg cgaccgaacc gtcggtatca ccgagaaacc gtctgcagag tttctggctc
     2101 gcctgggtga gcgctatggc ttccccccac ctcatgcacc tggacatgct gcaattgcca
     2161 gcatgcaagc aatatgtacg gggcaggctc gagcattgat ctgcatgggg ggcaactttg
     2221 cactggcaat gccagatcgg gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg
     2281 tacacgtagc cactaagctt aaccgctctc atctgctgac cgcacggcat agctatattc
     2341 tgccggttct ggggcgtagc gagattgata tgcaaaaaag cggtgcgcag gcggtaaccg
     2401 ttgaggattc aatgtcgatg attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa
     2461 tgctgaaatc agagtgtgca gtggtcgcgg gaatcgcgca ggcagcacta ccccagagcg
     2521 tggtagcctg ggagtatctg gtggaagatt atgatcgcat tcgcaatgac attgaagctg
     2581 tgctgccaga gttcgccgac tataaccagc gcatccgtca tcccggtggt tttcacctga
     2641 taaatgcagc tgctgaaagg cgctggatga cgccgtcagg taaggctaat ttcattacca
     2701 gcaaagggct gttagaagat ccctcttcag cgtttaacag taagctggtc atggcgacag
     2761 tacgcagcca cgatcagtac aacacgacga tttatggtat ggatgatcgc tatcgagggg
     2821 tattcggtca acgagatgtg gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa
     2881 aaaacggcga aagagttaat cttattgcgc ttacgccaga cggtaagcgc agctcacgcc
     2941 gcatggatag attaaaagtg gtcatttacc ctatggctga ccgctcactg gtgacctatt
     3001 ttccagaatc gaatcacatg ctaacacttg ataaccacga tccattaagt ggtattcctg
     3061 gctataaaag tattccggtt gaattagaac catcaaatta atgcctcttc tcatttcttc
     3121 tgctgtcatc cgcacagcag aagaattcct cattgactat tattgcgcaa tttgctcaca
     3181 tggattaaat taaactacat actataagat ataaacttct gcctacagct gtaagaaact
     3241 ccgctcagta ctgaagcacc agtcctattt cttcttttct ccagcctgtt atattaagca
     3301 tactgattaa cgacttttaa cgttatccgc taaataaaca tatttgaaat gcatgcgacc
     3361 acagtgaaaa acaaaatcac gcaaagagat aactataaag aaatcatgtc tgtaattgtg
     3421 gttgtcttat tactgacact tacgttgata gccatttttt cggcaattga tcagctgagt
     3481 atttcagaaa tgggtcgcat tgcaagagat cttacacatt tcattatcaa tagtttgcaa
     3541 gactggaaat aaacagcaaa ttataaatgt gaaatgttta aaaggtatcg ataaaacttt
     3601 attgttttaa ggagataaaa tgtcgctcgt ttgttctgtt atatttattc atcatgcttt
     3661 caacgctaac attttagata aagattacgc cttctccgac ggcgagatcc tgatggtaga
     3721 taacgctgtt cgtacacatt ttgaacctta tgagcggcat tttaaagaaa tcggatttaa
     3781 tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt
     3841 tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc
     3901 ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg
     3961 cctgtctatt tttgccgcat gcaaaggttt cattacacta ttaactaacg gtgtgctatc
     4021 cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa
     4081 agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac taaagcaaga
     4141 gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa aaaatttgat
     4201 acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacatctta
     4261 ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta
     4321 ccgttgtcaa aggcacacgg gtatgaatac gggcaacacg atgagtgctt tagctatttg
     4381 attatttgct aacgagtagt caaccacgca cgctacgtaa gaattatatg gggcagccat
     4441 tccctgcccc gcgttgtttt taggcgatat atttattgaa ataaataagt gacatccatc
     4501 acatatttat gcacttgcat aacctgttgc atgattattt atgatctcaa ttcggcattt
     4561 tgtcagtaaa atgcaataat ttattaaata tcaataaatt agttgtttat cggcgagaaa
     4621 ttacttaata gaacagaaag taatgtcaac gctttatgga ctgttatttc cc
//
LOCUS       DAJVPT010000193         4657 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVPT010000193 DAJVPT010000000
VERSION     DAJVPT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            99..283
                     /locus_tag="OQM47_003863"
                     /pseudo
     CDS             99..283
                     /locus_tag="OQM47_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            426..647
                     /locus_tag="OQM47_003864"
                     /pseudo
     CDS             426..647
                     /locus_tag="OQM47_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(668..730)
                     /locus_tag="OQM47_003865"
                     /pseudo
     CDS             complement(668..730)
                     /locus_tag="OQM47_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1180..2592
                     /gene="ospC1"
                     /locus_tag="OQM47_003866"
     CDS             1180..2592
                     /gene="ospC1"
                     /locus_tag="OQM47_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1481819.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2696..2846)
                     /locus_tag="OQM47_003867"
                     /pseudo
     CDS             complement(2696..2846)
                     /locus_tag="OQM47_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2922..4619
                     /gene="ospD3"
                     /locus_tag="OQM47_003868"
     CDS             2922..4619
                     /gene="ospD3"
                     /locus_tag="OQM47_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1481820.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1630 a          836 c          864 g         1327 t
ORIGIN      
        1 cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg gtaattgcag
       61 accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct ttgtgtccga
      121 tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag gtgaagaaag
      181 ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca
      241 gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac
      301 cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc
      361 cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg
      421 aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc
      481 aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg
      541 cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt
      601 tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg
      661 cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact
      721 cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc
      781 tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga
      841 tttcattgca gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa
      901 gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat
      961 tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat
     1021 tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc taattacatg
     1081 cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc ttgtagggaa
     1141 agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg
     1201 aactcagcaa atacccaatg caatatagat tctatggata acagattaca tacattgttt
     1261 ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta
     1321 aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc acagagttat
     1381 agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca
     1441 tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat
     1501 atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg
     1561 ctaattatta attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa
     1621 attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac
     1681 acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa
     1741 atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt
     1801 gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac
     1861 tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt agataaattt
     1921 tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta
     1981 ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt
     2041 agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg
     2101 gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta
     2161 agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca
     2221 gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct
     2281 ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt
     2341 gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta
     2401 cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt
     2461 gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata
     2521 aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat
     2581 aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc
     2641 gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt
     2701 tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga
     2761 gcagcccccc ctgaactgga atggccgttc cggatacccc cctgagtttc agagtaacgg
     2821 tgacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     2881 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     2941 tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     3001 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     3061 tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc tgtcgacatt
     3121 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     3181 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta
     3241 atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt attttttata
     3301 ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa
     3361 atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca
     3421 ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta
     3481 acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat
     3541 gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca
     3601 tgtcctccat aatcagatgg cctgataatt tacttcaccc caaagttatt tatcacgcga
     3661 tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc
     3721 tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat
     3781 tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga gaactcttgg
     3841 aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag
     3901 gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat
     3961 acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc
     4021 tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc
     4081 aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc
     4141 tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac
     4201 ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga
     4261 atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg
     4321 gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg
     4381 ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcagcag
     4441 aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa
     4501 taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa atagcagaac
     4561 acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata aaaagctgaa
     4621 tattatctcg tggtgataca ctgaatttga ggtaacc
//
LOCUS       DAJVPT010000194         4614 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVPT010000194 DAJVPT010000000
VERSION     DAJVPT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..285
                     /locus_tag="OQM47_003869"
     CDS             <1..285
                     /locus_tag="OQM47_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481821.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OQM47_003870"
     CDS             296..1036
                     /locus_tag="OQM47_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1481822.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OQM47_003871"
     CDS             1095..1481
                     /locus_tag="OQM47_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1481823.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OQM47_003872"
     CDS             1490..1819
                     /locus_tag="OQM47_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1481824.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..>4614
                     /locus_tag="OQM47_003873"
     CDS             1791..>4614
                     /locus_tag="OQM47_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1481825.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRR"
BASE COUNT         1138 a         1065 c         1485 g          926 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcg
//
LOCUS       DAJVPT010000195         4500 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVPT010000195 DAJVPT010000000
VERSION     DAJVPT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..919
                     /gene="tauA"
                     /locus_tag="OQM47_003874"
     CDS             <1..919
                     /gene="tauA"
                     /locus_tag="OQM47_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1481826.1"
                     /translation="IAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWRKFD
                     SGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTISKP
                     EDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYV
                     WAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQ
                     PYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAII
                     DTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            932..1699
                     /gene="tauB"
                     /locus_tag="OQM47_003875"
     CDS             932..1699
                     /gene="tauB"
                     /locus_tag="OQM47_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1481827.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1696..2523
                     /gene="tauC"
                     /locus_tag="OQM47_003876"
     CDS             1696..2523
                     /gene="tauC"
                     /locus_tag="OQM47_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1481828.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2520..3371
                     /gene="tauD"
                     /locus_tag="OQM47_003877"
     CDS             2520..3371
                     /gene="tauD"
                     /locus_tag="OQM47_003877"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1481829.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3477..4451)
                     /gene="hemB"
                     /locus_tag="OQM47_003878"
     CDS             complement(3477..4451)
                     /gene="hemB"
                     /locus_tag="OQM47_003878"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1481830.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          983 a         1184 c         1329 g         1004 t
ORIGIN      
        1 catcgctttt caggcacagg cggtgaacgt caccgtggcg tatcaaacct cagccgaacc
       61 ggcgaaagtg gctcaggccg acaacacctt tgctaaagag agcggggcga gcgtggactg
      121 gcgtaagttt gacagcggag ccagcatcgt gcgggcgctg gcttctggcg acgtgcaaat
      181 cggcaacctc ggttccagcc cattagcggt tgcagccagc caacaggtgc cgattgaagt
      241 cttcttgctg gcgtcaaaac tgggtaactc cgaagcgctg gtggtaaaga aaactatcag
      301 caaacccgaa gatttgattg gtaaacgcat tgccgtaccg tttatctcca ccacccacta
      361 cagcctgctg gcggcgctga aacactgggg gattaaacca gggctagtgg agattgtgaa
      421 cctgcagccg cccgcgatta tcgccgcatg gcagcggggt gatattgatg gtgcttatgt
      481 ctgggcaccg gcggttaacg ccctggaaaa agacggcaag gtgctgaccg attctgaaca
      541 ggtcgggcag tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag attttgccga
      601 gaaacatcct gaggtcgtga aagcgttcgc caaaagcgcc atcgatgcac agcaaccgta
      661 cattgctaac cctgacgcgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt
      721 aagcggcgtg cctgaaggtg acgttccggg gctggtgaag ggaaatacct atctgacgcc
      781 gcagcaacaa acggcagaac tgaccggacc ggtgaacaaa gcaatcatcg acaccgcgca
      841 gtttttgaaa gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac
      901 ctcgcgcttc gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tctatgccga
      961 ttatggcggc aaaccggcac tggaagatat caacctgacg ctggaaagcg gcgagctact
     1021 ggtggtgctg gggccgtccg gctgcggtaa aaccaccctg ctgaatctga ttgccggttt
     1081 tgtgccttat cagcatggca gcattcaact ggcgggtaag cgtattgagg gaccgggagc
     1141 agagcgtggc gtagtttttc agaatgaagg gctgctaccg tggcgcaatg tacaggacaa
     1201 cgtggcgttc ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca
     1261 ccagatgctg aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct ggcagctttc
     1321 cgatggtcaa cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt
     1381 attactcgac gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaactct
     1441 gctgctgaaa ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga
     1501 agaagcggtg tttatggcga ctgaactggt tctgctttca tccggccctg gccgtgtgct
     1561 ggagcggctg cggctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat
     1621 caagtccgat ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca
     1681 acgggaggcg ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa
     1741 tggcgctggc cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc
     1801 actgtatggt ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg
     1861 caacaagtac tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg
     1921 ctgtggcagc atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg
     1981 gtgatcggta ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg
     2041 gatccggtaa tcgaacttta tcgtccggtg ccaccgctgg cttatttgcc gctgatggtg
     2101 atctggtttg gtattggtga aacctcgaag atcttactga tctatttagc gatttttgcg
     2161 ccggtggcga tgtcggcgct ggcaggggtg aaaagcgcgc agcaggttcg cattcgtgcc
     2221 gcccagtcgc tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg
     2281 ctgccggaaa tcctcaccgg attacgcatt ggtctgggtg tgggctggtc tacgctggta
     2341 gcggcggagc tgattgccgc gacacgcggt ttaggattta tggttcagtc agcgggtgaa
     2401 tttctcgcga ctgacgtggt actggcgggg atcgcggtga ttgcgattat cgcctttctt
     2461 ttagaactgg gtctgcgcgc gttacagcgc cgtctgacgc cctggcatgg agaagtacaa
     2521 tgagtgaacg tctgagcatt accccgctgg ggccgtatat cggcgcacaa atttcgggtg
     2581 ccgacctgac gcgcccgtta agcgataatc agtttgaaca gctttaccat gcggtgctgc
     2641 gccatcaggt ggtatttctg cgcgatcaag ctattacgcc gcagcagcaa cgcgcgctgg
     2701 cccagcgttt tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg
     2761 agatcatcgt gctggatacc cataacgata atccgccgga taacgacaac tggcataccg
     2821 atgtgacatt tattgaaacg ccacccgcag gggcgattct ggcagctaaa gagttacctt
     2881 cgaccggcgg tgatacgctc tggaccagcg gtattgcggc ctatgaggcg ctctctgttc
     2941 ccttccgcca gctgctgagc tggctgcgtg cggagcatga tttccgtaaa tcattcccgg
     3001 aatacaaata ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg gtcgcgaaaa
     3061 atcctccgtt gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt
     3121 ttgtgaatga aggctttact acgcgaattg tcgatgtcag cgagaaagag agcgaagcct
     3181 tgttaggctt tttattcgcc catatcacta aaccggagtt tcaggtgcgt tggcgctggc
     3241 aaccgaatga tattgcgatt tgggataacc gcgtgactca gcactatgcc aatgccgatt
     3301 acctgccaca gcggcggatt atgcatcggg cgacgatcct tggggataaa ccgttttatc
     3361 gggcggggtg atacgagtgc ggacggttcc ctcgccccct tggggagagg gttagggtga
     3421 gggggcgttc actgtacttt caacaggtta actccccctt tctgagagga aaaaaattaa
     3481 cgcagaatct tcttctcagc caaatccagc gcaaagtagc tgaaaatcag atccgcaccc
     3541 gcacgcttaa ttgaacctaa gctttcgagc acgactttct cttcatctat agcacccgcc
     3601 agcgcggcga acttaatcat cgcgtactca ccgctcacct gatacgcgcc aatcggcaat
     3661 tcagtacgtt cacgcagctc acgcacgatg tcgaggtacg ctccagcagg tttaaccatc
     3721 aggcagtctg cgccctgggc ttcatccagc agtgactcac gaatcgcctc gcgacggttc
     3781 attgggttca tctgatagct tttgcggtcg ccttttaatg cgcttcctgc agcttcacgg
     3841 aacgggccat aaaaggaaga ggcgaacttg gtcgaatacg acataatcgc cgtatcttta
     3901 aagcctgcag cgtccagcgc ctgacgaatc gcctgtacct ggccgtccat cgcggcggaa
     3961 ggggcgatga agtctgcgcc tgcagcagct gcaaccacgg cttgcttgcc taaattttcc
     4021 agagtcgcgt cgttgtcgac gccatgctcg cacagcacac cgcagtgacc gtgagaagtg
     4081 tattcacaga aacaagtgtc tgacatgacg atcatttctg gcacagtctg cttgcagatg
     4141 cgcgacatac gcgccaccag tccatcttcc cgccaggcgt cgctgccggt ttcatcggta
     4201 tggtgagaga tgccaaaagt cattacggaa cggataccgg cgttggcgat gcgttcaatt
     4261 tcgcgtgcca gatgtttctc tggaatgcgc atcacgcctg gcatggcttc aacggctttg
     4321 tagtcgtcaa tttcttcttc aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt
     4381 gtctcttcaa acatagcgcg cagcgcagga gatttgcgca ggcgacgagg gcgttggatt
     4441 aagtctgtca tggtctgcct gatgtttgtg gaataaggtg cacagtatac ctgaagcggg
//
LOCUS       DAJVPT010000196         4379 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVPT010000196 DAJVPT010000000
VERSION     DAJVPT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(231..476)
                     /locus_tag="OQM47_003879"
     CDS             complement(231..476)
                     /locus_tag="OQM47_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481831.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1253..2095)
                     /locus_tag="OQM47_003880"
     CDS             complement(1253..2095)
                     /locus_tag="OQM47_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCS1481832.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2180..2377)
                     /locus_tag="OQM47_003881"
     CDS             complement(2180..2377)
                     /locus_tag="OQM47_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481833.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2397..2885)
                     /locus_tag="OQM47_003882"
     CDS             complement(2397..2885)
                     /locus_tag="OQM47_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481834.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2882..3259)
                     /locus_tag="OQM47_003883"
     CDS             complement(2882..3259)
                     /locus_tag="OQM47_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1481835.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3306..3683)
                     /gene="cbtA"
                     /locus_tag="OQM47_003884"
     CDS             complement(3306..3683)
                     /gene="cbtA"
                     /locus_tag="OQM47_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCS1481836.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3763..3984)
                     /locus_tag="OQM47_003885"
     CDS             complement(3763..3984)
                     /locus_tag="OQM47_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1481837.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4071..>4379)
                     /gene="radC"
                     /locus_tag="OQM47_003886"
     CDS             complement(4071..>4379)
                     /gene="radC"
                     /locus_tag="OQM47_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1481838.1"
                     /translation="EEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNA
                     AAVVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL
                     "
BASE COUNT         1026 a         1040 c         1149 g         1164 t
ORIGIN      
        1 ggcttttttc cgtaatgctc taacttccac gcattggttt ctgacatcaa ataaggctat
       61 gactttctct cccttttaat ctcattctga tctaaaacca tatcttaaaa aaatgcatgg
      121 gtattttaat tattttgatg ttgatctaac gcactcaagg accattttat taccgattat
      181 ccattctatt taactgttgc gatataaatg tttgttttag gtgctttaat tcagtacaag
      241 tcatcaaacc gtaccagacc ctgactgtca gtgacaccga caggtggggg cggcggtgtg
      301 gggtcaaact gagtgtgtct gcctctggcc cacagagcca gctctgccgc cattgcccgt
      361 ggatgatact ccagctgcac gttgagaccg gaaactgggt tgttgttctc atccacgaac
      421 tgcagcgcca gccagtgcat ccgctgctta ccgcaggcga cttctttgta cttcattttg
      481 tgactcaggc aggtgactgt cgtgttgcct gttgcgtcca ttttgcggtt aaaccctttt
      541 aattcaattt gttaaattgc tttccacctt ttgcggggcg cgacttaacc aaaatattca
      601 ctgttatcat aatatgcaat gggctggagg ttgcaggtca tttagggatt ttctgtaagc
      661 cccgttttgc agaacgttct atctccgatt gagatcctgt ctctcggtat taccggtagt
      721 ctggccataa tcccctatgg atcatattga tatagtaggc ataacaccca aaagttgact
      781 aaagctaaat caatcagagt gaaaaatgtt cggtttgatg ttggggggga ctgaggaggg
      841 gccaacgcag gtggtatgcg ttttacttca gaccggacaa agaccggatg tatccgtgca
      901 gtaatgaatc cgttagccag gtgctcatgt ggaaatagtg tctattgggg atttgaagtt
      961 tgacagaaaa ggccacagtg accagaaggg ggctacttag aagaaatgtt caggctgggt
     1021 ggaagaggta aagcagtgac atcagcattc aaagtgaccg gatatctacc agaaccccag
     1081 aaagcaaaaa accagcccgt aggctggttc ttctaaatag tggtgccgga ctcggaatcg
     1141 aaaatttctt ttatcatatt gatattaatg caatttgttt ggtttgatta aaaatgtacc
     1201 ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcactctcgg gatcaggctg
     1261 agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc agttccagcc
     1321 ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac atctcgtctt
     1381 catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata gtgatgtttt
     1441 cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc aggtcgacga
     1501 gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac ctcaccaggt
     1561 tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa tcccagctca
     1621 gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc tggtgcagtt
     1681 gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca tattccagtg
     1741 acctgtacca tgtgtcccgg gtctgcgcat ccatcagact cagcatcccg gaacgttgca
     1801 tcagatcacg ccagagttcg cgatcaagat tgcgtgtgat ggctttcatt gcggtttccg
     1861 gtttctccat cagccagcaa ccgcagcgaa aatcctgttt catggcccag tccagagccg
     1921 ttttacctct aatactttct gtcattgctg atacttcagc cagttgtcgg ataagtgtgt
     1981 cgatttgcag caatgtactc tttcgtacag ccagaatgtg gcggatactg gttgagcaga
     2041 taacgtccgt atgcccgttg accacttccg gacgagaggc tgtgatttca gacataatcg
     2101 atattccttt ttgcagataa acgaaaacgc cagccgtatt caggctggcg tcagtggaat
     2161 gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagctga cgcagggtac
     2221 ggatatcttc cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc gttttatcct
     2281 catcgttgtc gaagataatc cctgattcgc agtcaatatt gtcctgcagc cacgcaatca
     2341 gaatatccag cgccgtttct gtggttaata atttcatcca gtgtcttttg tcctgattac
     2401 cagtcgaaag tggggaaccc cggcagacat tctgcgccgg cgtccagatg aatgagactg
     2461 acgccataac gccgggtgag agtgacgacc agacgacgga acgttctgga caggcccagg
     2521 cgtttaagcc gcagaaccgg gtacgaccat gcatcggtac gtaacagata gccgctgcct
     2581 gtaaaatgaa tccattctgt ttcgccgtcg tcattagtct ggtgtgacag cgagtacagc
     2641 caggcgttgt ccgtttccgt gatatgtgcg gtgctgcaac gtatgcaggt aagggttgtt
     2701 aacggtggtg gtgtactggt tgcgtgttgg tcggatttat cgactacgcg gagtgaataa
     2761 ccgttttcag agaccttgtt aatcagttca ctgagattaa caccatcgac gtcaacgaca
     2821 atgcgcccca tgttcagtgc ctgcacgtta acgctgtcgg cttccagcgt cagggccagt
     2881 ttcattgttt cgcttccgga tgcttccccc gggtaatgtt atttaccgtt ctgtagttat
     2941 cgcgggtcat caggccggtt gcccggcgtg cccggagaat atcaatgctg ttgattaact
     3001 gcgacggggc tcctgcgctg aatcccggct ggtcggtacg taccagcgcg tatttttcaa
     3061 cgagaaagtt caccgcgtca cacagtgaaa ttcctgcctc aatatgctgc tcaatcacac
     3121 gttcatcagc gaacggtgtg tcattcagcg tgaggccata gtgctggtcc agcagtcggg
     3181 tgagcagtgt ctgccagatg gtgatgggag acgggcagcc cgatgcctcc cgtacgtgag
     3241 tgtcgggtaa tgtattcatc ttaagttctc tgtaacaggt ggttataaag gggagatatc
     3301 cggtgttata cggtggttgc gggcgctgcc ggtgtcggat aaacagccag ataaacgtaa
     3361 ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag tgtgacggta
     3421 tgctgatggc ggggattcag ttcaccgcca gtgagcatga gttccagttg tttcatcagc
     3481 agcggaaagg cctggtccag gtggtacgca tctacgtcgc tgaacctgcc tctgataccg
     3541 gcgcggtctg caaggtaatg caggcggtta ccctcctgta ccagtcgggc cccgaaacag
     3601 ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgtcgtc gggaggcgtt
     3661 gtcccgggga gtgtgtctga cacgataaag tcctcacaga aaatcggtta agaatggtcc
     3721 ggtagtggcg ataattctgc tcatcagaat tcccttccag actcagttca gggtgacgct
     3781 catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca cagccagtac
     3841 accgggaata tcctgcactt cacggccggt atatgtatcc gtgctaccgt gccagcggta
     3901 tttaccggta cagaacggaa aaagacggga tgcaggatgc tgacggtgaa tacgcatggc
     3961 ttcaccacgg gtgatgattt tcatgagaat agtcctcctg aatgtgaaag taagaaacag
     4021 ctgagaaaga gaaactgaag tgatatgaag gtgattgtga cggtgacggg ttaaagcaga
     4081 ccgtgttccg caaaggagaa aacctggctg ccaccgacta tcagatggtc cggtacccgg
     4141 atatcaacca gggccagtgc ctgtaccaga cgctcagtga taaggcggtc tgccttgctg
     4201 ggggggactt caccggacgg gtgattgtgc gccagcacca cggcagcggc attgtggtac
     4261 agggcgcgtt taatcacttc ccggggatgg acttccgtac ggttgatggt gccggtgaag
     4321 agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg gaactcttc
//
LOCUS       DAJVPT010000197         4298 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVPT010000197 DAJVPT010000000
VERSION     DAJVPT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..2419
                     /gene="mutS"
                     /locus_tag="OQM47_003887"
     CDS             <1..2419
                     /gene="mutS"
                     /locus_tag="OQM47_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1481839.1"
                     /translation="QLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAI
                     CEQIGDPATSKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDI
                     SSGRFRLSEPADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDT
                     ARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDS
                     IIMDAATRRNLEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLER
                     QQTIGALQDFTAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQL
                     ETVDSAPVQALREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALA
                     DGATDYLERLEVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNA
                     ERYIIPELKEYEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLV
                     NLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITG
                     PNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMV
                     EMTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYF
                     ELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKR
                     ARQKLRELESISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWI
                     YRLKSLV"
     gene            2525..3177
                     /gene="pphB"
                     /locus_tag="OQM47_003888"
                     /pseudo
     CDS             2525..3177
                     /gene="pphB"
                     /locus_tag="OQM47_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3228..3995)
                     /locus_tag="OQM47_003889"
     CDS             complement(3228..3995)
                     /locus_tag="OQM47_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1481840.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4191..>4298
                     /locus_tag="OQM47_003890"
     CDS             4191..>4298
                     /locus_tag="OQM47_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1481841.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGAD"
BASE COUNT         1053 a         1122 c         1116 g         1007 t
ORIGIN      
        1 gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat
       61 ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga
      121 gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg
      181 caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt tacaggagcg
      241 tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct
      301 ggatatcagc tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc
      361 agagctgcaa cgctctaatc cggcggaact gctgtatgca gaagatttcg ccgagatgtc
      421 gctgattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac
      481 cgcgcgccag cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga
      541 gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca
      601 acgcacgacc ctgccgcata ttcgttctat caccatggaa cgtgagcagg acagcatcat
      661 tatggatgcc gcgacacgtc gtaacctgga aattactcag aacctggcgg gcggtgcgga
      721 aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa
      781 acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat
      841 tggcgcattg caggatttca ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct
      901 ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat
      961 gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc
     1021 accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga
     1081 gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg
     1141 ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga
     1201 gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctaaaag ttggctttaa
     1261 tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa
     1321 ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga
     1381 gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta
     1441 tgaagagctg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc
     1501 gctggcggaa ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac
     1561 ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt
     1621 tgaacaagta ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg
     1681 tatgttgatt attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc
     1741 actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg
     1801 acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc
     1861 aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag
     1921 tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc
     1981 gtgggcgtgc gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca
     2041 ctatttcgag ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga
     2101 tgcgctggag cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag
     2161 caaaagctac ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg
     2221 cgcacggcaa aaactgcgtg agctggaaag catttcgccg aacgccgccg cgacgcaagt
     2281 ggatggtacg cagatgtctt tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc
     2341 actggaaaat ctcgacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg
     2401 cttgaagagt ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg
     2461 acaactgacg aatcaaataa aaataccctg tataatagga aagcttattt tacagggtaa
     2521 aaccatgcca tctacacgct accaaaaaat caatgcccat cactatcgcc atatatgggt
     2581 cgttggtgat attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt
     2641 tttccccgaa accgacttac ttatttctgt cggcgataat attgatcgtg ggccggagag
     2701 tcttgacgtc ctgcgcctgc taaaccagcc ctggtttatc tccgttaaag gcaaccacga
     2761 agcaatggcg ctggatgcgt tcgcgaccgg cgatggcaat atgtggcttg ccagcggtgg
     2821 tgactggttt ttcgatttaa atgattgcaa caagaggcaa tagttctgtt gctgaaattc
     2881 catcaccttc cacatattat tgaaatcact aacgacataa taaaatatgt catcgcacat
     2941 gcagattatc cgggtgatga atatctcttt ggcaaagaaa tagcggagag cgaattactc
     3001 tggcctgttt atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat aaacggcgct
     3061 gattatttta tatttggaca tatgatgttt gataacattc agacgttcgc taaccagatt
     3121 tatattgata ccggatcgcc gaaaagtggg cggctgtcat tttataaaat aaggtagtct
     3181 catgcttctt ctgtgaagca tgagtaacca gatgttattt caggccatta ttcatttttc
     3241 gctaccagca aagagagatc ctgcttcgcc agcgcgcgac tggcactctc cggcaaaccg
     3301 tcgtctgtaa taatctgatc aaactcgctt aatggtaacg ccagccatgt cgccacctga
     3361 ccatatttcg tcgcatcaca gaccaaaact cgctggcggc tggcactggc aatcgcccgt
     3421 ttcaccgtga ctttatcttc cgctggcgta gaaatccccc gcacactcca tgacgatgca
     3481 gaaataaaag cctgatcaat catcaggctg cacagcatgg tcgcagcggc ttccccgaca
     3541 caggaacggt tttcccgaca cactgcaccg ccagtgtgaa taattgtgca attactgttg
     3601 tcgagcaagt agttcgcaat aacgaaatcg tttgtgacca cagtcagtga ctccatgtga
     3661 atcagatgct gtgctatcgc taacgtggtc gttcccgcat ccagatagat acaacttccc
     3721 ggcagaacaa gacttgccgc cagcttgcca atagccgctt tttgcgtcat tgccagcgca
     3781 gtttttacct gatgagaagg ttcatgcgcc acgcgtcccg gagactggac gcctccggac
     3841 accagcacaa cggctccctg ctgctccagt ttttgtaaat cccgacgaat ggtcatatgt
     3901 gacacattaa ttctgtccgt tagttcagca atactgacaa tgcctttttc agctaccatc
     3961 tcaaggatga tttggcgacg ctctacgggt atcaactttt gctccttcct ttgtcctgct
     4021 gacattctac gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt agttcacata
     4081 aaataacaca aaatgttaat ttatgtgaat catctcacca tatcgttatc tcccagcgct
     4141 tatgttcaca atatcaaaca aaatatcact taaattaaca aggagagcag atgaaaacgg
     4201 gatctgagtt tcatgtcggt atcgttggct tagggtcaat gggaatggga gcagcactgt
     4261 catgtgtccg cgcaggtttt tctacctggg gcgcagac
//
LOCUS       DAJVPT010000198         4284 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVPT010000198 DAJVPT010000000
VERSION     DAJVPT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            40..1068
                     /locus_tag="OQM47_003891"
     CDS             40..1068
                     /locus_tag="OQM47_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1481842.1"
                     /translation="MVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISNYAMPGE
                     TTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVTNIESDT
                     ATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKWFIALMG
                     LPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPVIVLIMT
                     AALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLLIANKPG
                     FVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAARVFVND
                     ERFTRGGN"
     gene            1068..1802
                     /locus_tag="OQM47_003892"
     CDS             1068..1802
                     /locus_tag="OQM47_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1481843.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2270..3031
                     /locus_tag="OQM47_003893"
     CDS             2270..3031
                     /locus_tag="OQM47_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1481844.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3075..4154
                     /locus_tag="OQM47_003894"
     CDS             3075..4154
                     /locus_tag="OQM47_003894"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1481845.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT         1191 a          815 c         1057 g         1221 t
ORIGIN      
        1 acacaaagcc gtgtcggcct tcttggttta ttttgcgtca tcgtgctggc cgctctcgcg
       61 gacagacccc gctggcgcgg acgtcccctt agcggtctgt tgcgcttgat cttcgtcgcc
      121 tccttgctgt gctggtgtgt cggtatctca aactatgcca tgccagggga gacaacggct
      181 gactttacgc attcatcatc gaatgaccag cgtatacgta ttctgaaagg tgccataagt
      241 cttattcaac agcgcccctt aaccggttgg gggttaggca gctttgaagc gaccttcccg
      301 caagccttag aggatgtggg cgtgacaaac atagagagtg atacggctac gcatccccat
      361 aacgaactgc tgttcgtaac ggctgaaggt ggcatcgtcg ccttgtttgg ttttttgctg
      421 ctatcaggcg tttggctgtg gccgtgtata tgccgcctga aaatgacaaa caataacgcg
      481 caccatcaat gtgaaaagtg gtttattgcc ttaatggggt tgcctttagt cattcatatg
      541 atgaccgaat atcctctcta tacgtctgtt ccccatttga tgctgttgct gctccttttc
      601 cgcatcggaa tgccggaaaa cgtgatgaat caggtacgta tacctaaaac agtccggttg
      661 ggtattctgc ctgtcattgt gctcattatg accgccgcgc tggtcatcct ggtagcggga
      721 tttgatgcac agcgggaact gaccagagct gaagctgata tgaacgaagg atttatccct
      781 cgccttccgg aacatagctg gaaatacctg acgcaggcag aacgtcttga gcgcgatcag
      841 catctgctta ttgcgaataa accggggttc gttcgcgata cggaaagcat gtctcttttc
      901 actgtgtggg gaaataaatg gttggcaatc cacaatgatg cagacgtaag cgctgcaatg
      961 atattcattg cgcacaggcg cggtgattat ctgacagctg aaaaaataag aaaacgcgca
     1021 gctcgggtat ttgtaaatga cgaacgtttt actcgcgggg gaaactaatg gttaacgatc
     1081 tgacgtataa gaaattagaa agcacaaaca tcaactgtaa tattctctcg ccagaaaata
     1141 ccacatggac aaatgtcacg gtgattggcc tggatctttt tgccatttgc ggcgtgtgca
     1201 gtttgctcaa gcgtgttggg ggagtgaaac ttcaggtttt cacttcctta cccactgata
     1261 tgaatgttct gactgacagt agcatcgtga tatgggtaaa aatgcgtcac gatggtttgc
     1321 ctgaattagc cggacatatt gtgaaggttt gtcgtcgata ccgtctgatg cggcaactcg
     1381 ttattagcga tgctattcct gaaggaatga cgaatgatat cgggcctctt tcccgagtat
     1441 ggcttactca tgggagtgca aaagtggata ttttattatc ttcattacgg aaggtgttaa
     1501 atgctcattg tttgcctggt cctttgttta taaaacgcct gggaagagtg caatggaggg
     1561 ttcttttact ccgcgcgaag gggattagca cacgtaaaat tgcagaaatc tgtggtattg
     1621 gcctcaagac cgttagcgcc catgagtccg caattcgaga acgattaggc accaggaata
     1681 aaagtgagta cgtctggtta gtacgtaatg ctcaacttat acaacaggcg atacccgcgt
     1741 tgggaagaga tgcgtcaagg tatgaaagat gtaacaatat gcgaacaaag caaaaagtat
     1801 aattgatgcg tcatagataa ctctcttttt cgatgacgaa atcgctgagc atgcgtggtg
     1861 aggttagtaa tagatgggtg ctatgttttg ccacgggttt gtagctgtga aacctggaaa
     1921 aaaataagaa ttaaaaatat ttaaagagag ttaataaggt tctggttgta attgtcttgg
     1981 tcaaaaaaaa gatattgtca atgttgtttt ttaaattttc aggttttggt taaatatttg
     2041 cttgcaaata taggtgattt ttttattgta ggcttttgaa ggctattata actgtgcgct
     2101 aaattaatgc gttgaataaa agaaataaca aaattaagaa aagccatatt tacgatatgg
     2161 gcaacataaa tataatctaa aaaagagtaa ggcgttgtag gttggttccg cgttcattac
     2221 cttgttagtg aacgcggatt tatcagttgt cgaaatggtt tttataataa tgaaaaattt
     2281 taatgtacta accaaagtcg tattttgctt gtcgttttta tcaagcgcat ttcccaccta
     2341 tgctgcggat gatgtcaaag tgactcaaaa cacttcagtt tttaaactga agcttagtgc
     2401 aacgcgatta atctatgaac aagggtcgga tggcgcagcg ataactgtga caaatccgca
     2461 ggattggccg atgcttgtgc agagtaaagt ctttgaagaa gataaagtaa ctgcagcgcc
     2521 ctttattgtc actccgccac tgttcaggct taatccaggc cagcaaaata aaatgtgggt
     2581 tgtgcagact gcgccagctt tacaagacag ggaaaaatta cactggctat gcgccaccgg
     2641 cattacgcct aaaaagagtg atgtttgggc cgagaaagat aaagaaaaaa ttgctgaaaa
     2701 tgtgaacctg aacatccagg ttaacgtaca tacctgcata aaacttatta ccaggccatc
     2761 tggtattgaa ggcaagatgg ttaatgcgag tgagaagtta tcgtggaaaa agcagggtga
     2821 taaaattgtc gcggtcaatc agtcgccgtt ctacatcaac ctttcttctt tacaggttgg
     2881 cggtagcaag attgagaaac tagattatat tgcgccgttt tcgcaacatg agtttgttat
     2941 gccaaaaact agcggccagg atattaaatg gtctgtgatt actgatgaag gtggtgagag
     3001 tcgtttgttt atatctcatt tgcaatcata atgtattaca tttttattga atatttttat
     3061 gccatgcata ttccatgttt tatcgactgt cattaatttt gataatggcc ttacttgcgg
     3121 gtcagttaag tgcccaggag tggtcatttg attcttcaca gttggaagga aacgttagtg
     3181 cggacactgt ggcaatgttt aatcagggag agcaactccc cggaaattat cgtgtggaaa
     3241 tttatcttaa cggtgaaaaa gttgatgttg gggagtttcc ctttcatcgt ccggaatccc
     3301 ctgaagaaaa agagcttgtt ccatgtttaa ccgttgatga ccttatccac tatggcatta
     3361 aaattgataa aagcagcagt gatactgaca ataaaaagaa tcagtgtttt aaatggaact
     3421 ctattgaagg tttgaaagta aattatgatt ttgatagcca gcgcgtacag ataaccgttc
     3481 cacaactgta tctgcaagat aaaaaaagta gtttagcacc agtgtcgtta tggaatgagg
     3541 gtgtagctgc attccgcatg gtgtatcaga caaatattga tatttccaaa caaaatgata
     3601 atcaatctac cacgcgtaat tctcgttatg gcaggttcac tccagggttt aatcttggtg
     3661 cctggaggtt tcgtagttca gtgacatgga gcaaggaact tggccagtca gaaagatggc
     3721 aaaggggtta catgtggttt gagcgaggca taaatgcgat taaaagccga ttaacgcttg
     3781 gagaatctta tacgtcgtct gaggtgtttg acagcatacc ttttcgcggc gggatgttag
     3841 cgaccgacga tgcgatgact ccgccggagg acagctacta cacacctgtg gtgcatggaa
     3901 tagcacaaag tgaggcgcag gttattatta aacaaaatgg gcaaattatt tttacccgtt
     3961 ctgtgccgcc agggcctttt gcgctggata atttaccaac gctggccgtt ggcggggagc
     4021 ttgatgttac agtgagagag agtaatggcg aggagcagta tttttccgtg ccatttcaaa
     4081 cgccggccat cgctctgcat gaaggatatt tcaaatatag tgtgatgggg gggaatataa
     4141 agaagaaggt ataaaaagct cccctgtctc cgtgggcgaa atcacggcga tgtacgggct
     4201 tccttatggc gtcacggtgt atggagggat tcaaaacgcc actcatttta atgcaatatc
     4261 tacgggtatt ggcatttctc tggg
//
LOCUS       DAJVPT010000199         4221 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVPT010000199 DAJVPT010000000
VERSION     DAJVPT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..927)
                     /gene="uspE"
                     /locus_tag="OQM47_003895"
     CDS             complement(<1..927)
                     /gene="uspE"
                     /locus_tag="OQM47_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1481846.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVE"
     gene            complement(1079..1831)
                     /gene="fnr"
                     /locus_tag="OQM47_003896"
     CDS             complement(1079..1831)
                     /gene="fnr"
                     /locus_tag="OQM47_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1481847.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2026..2541)
                     /gene="ogt"
                     /locus_tag="OQM47_003897"
     CDS             complement(2026..2541)
                     /gene="ogt"
                     /locus_tag="OQM47_003897"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1481848.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2552..2695)
                     /locus_tag="OQM47_003898"
                     /pseudo
     CDS             complement(2552..2695)
                     /locus_tag="OQM47_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3085..3900)
                     /locus_tag="OQM47_003899"
     CDS             complement(3085..3900)
                     /locus_tag="OQM47_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1481849.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            3997..>4221
                     /locus_tag="OQM47_003900"
     CDS             3997..>4221
                     /locus_tag="OQM47_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HCS1481850.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIIL"
BASE COUNT         1100 a          991 c          993 g         1137 t
ORIGIN      
        1 ttcaacgggt gtctgatact ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc
       61 aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt
      121 gcccagaacc acaattcccg cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg
      181 cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc gcagggcttt
      241 cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcgggt caaattccgg
      301 cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat gaacttcggt
      361 atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat tgagcgcatt
      421 atgatacggc tcttcactgg cgagattcac cgccaccagc gccttgcctc cttccggcca
      481 cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacagat gccagtccgt
      541 tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta gcaccaaatc
      601 gtgtccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat gccagaccac
      661 tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt ggatccaggc
      721 tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg agagcagggt
      781 ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc caccaatccg
      841 ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag gatcgataac
      901 aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg tcaacgcagt
      961 ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc ttctgccaga
     1021 tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg cggaaaaatc
     1081 aggcaacgtt acgcgtatgg ccagcaagct gggccagcgc atcgttattt tcgatggtga
     1141 tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc agacggctga
     1201 tggtttctac cgtcaggccc agatagttac caatatcgcc acgagtcatc gtcaggcgga
     1261 attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag atgaatgcag
     1321 ccagacgttc ctcggcattt ttcttcgaca acagcaagat catgtcctga tcgcctttga
     1381 tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta ccggacaaat
     1441 cgtccagcgt ttcgaacgga atttcacata ccatcgaggt ttccagcgcc tgagcgaagc
     1501 tcgggtgatg gccgctgccg atggcgtcaa atcccactag gtcgcctgct aaatggaaac
     1561 cagtgatttg ctcgtcgcct tgctcagtga ttgtataact tttaatcgta ccggagcgga
     1621 tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct ttctgaatag
     1681 gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg agtgtgaacg
     1741 ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg ccagactgaa
     1801 tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa ttgatatttg
     1861 tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac agcagagaat
     1921 tccgatatta gatgtaaata tatgtctatc tatttgaaac tccttaagtt gtcaagggta
     1981 actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca gcaaaagata
     2041 accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc cggtcatggt
     2101 gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat tcgatccgtt
     2161 tgccgcacca acggcacgcg ccgcgccagg gcggcccaat tgttcagcca gttggccgta
     2221 atgcattacc tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg
     2281 cgtccccccc gtggcagtgg gaagcgtatc aataatgcta agattaccgg caaaatattc
     2341 acgaagcttg tcgcttaaac cacctgggtt ggtggcagaa atgcgctcat agccttcttt
     2401 gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc attcaaccgc
     2461 ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggcg tggcaatttt
     2521 ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa ataccctgac
     2581 ctatcggcag accgacaaga taccacgcca gcaacatcag cagccatacc accaaaaaga
     2641 taagtggata gggcaagact aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc
     2701 agtctgatag cataaagcga ttttttaatt tttctgcctg aagaaatgag ggcaagccat
     2761 cacatggatc gagggtggga taattccagt cagtgaaatt ttaccgtagg ctatctgtta
     2821 tttgattctg tggcgtgata gccgccagac accactgaac tcgtcactca ttccccaatc
     2881 ccggcgagaa tgtggttatt tacagagcta aaaaatgaat aaaaaaatag agcctgtcga
     2941 catccgccag actctacagt acacacagca gtgcatcctc gtcttaaatc cggcctgggg
     3001 tcctggtgac cggtgacagt gttataacgg ttttacccat ccgttgccaa ttttcttcag
     3061 tgacagattt cacgaaaata taagttaaat catttgtctt gtgattgcat aattatgcat
     3121 cgttaatcga cgagtaaatt taccgggcga gtagcccgtt cgacgcttaa acatattgcc
     3181 gaacgattca gctgaggcat agcccagacg atgggcaatt ttggtcaacg aatatccatc
     3241 atcaagaagc gtcactgcag aaataacgtg catttgctga acccagtggc gaaatgttaa
     3301 tcccgtctct ttaacaaata agcgcatgag tgtacggcta ctgacgttaa ttttatccgc
     3361 tagattatcc agtgttggca gatacccttg gttgtttagc aaattctcac aaagctgctg
     3421 tagccgctca tcatttggcc aagggagttc aagtgacatt ttcaccccca tttttatttc
     3481 ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt tgtttatctg tggtactttg
     3541 cgacggtgga agttggttaa ttgcaattag taattccctt aacaggggtg aaatacccgt
     3601 catgaagcaa actgttccca gcgtttcact ttccgcttct gaaaccagta cggtttttaa
     3661 aagtacgttg cctgtcgcaa caaacttatg cggcatttgt gggggcagcc aaagcgcgct
     3721 gctggcatgg actagccaga cgctttctcc agcccataca cgaacgactc cttcagccgg
     3781 gtaaagcaac tgggcataga gatgtttatg ccagggaact gtacctccat tcagatgttc
     3841 cgaatccagt ccgataatgt gatgccctgg tgatgctttg ggttgcagtg taattgtcat
     3901 ttacagtagg tattttgtca gaaatcatca aataagccat gttaaatttt aaatatgacg
     3961 tatgcaagtt tccggatata tacggtgtaa agagtgatga taatttctaa aaaacagctt
     4021 attggggtgg tagcgatcgg tattcttttg actggcgttg tcttttttat ctggagggta
     4081 agcaaaggaa gatttataca gacgacggat gatgcttaca ttggtggtaa tatcaccacg
     4141 gtggcaagta aagtttctgg ttatatttct gctatagagg tgcgcgataa tcagagcgta
     4201 aaaaaaggcg atattatcct g
//
LOCUS       DAJVPT010000200         4209 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVPT010000200 DAJVPT010000000
VERSION     DAJVPT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM47_003901"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM47_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1481851.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQM47_003902"
     tRNA            complement(324..399)
                     /locus_tag="OQM47_003902"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQM47_003903"
     tRNA            complement(406..480)
                     /locus_tag="OQM47_003903"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQM47_003904"
     tRNA            complement(597..681)
                     /locus_tag="OQM47_003904"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQM47_003905"
     tRNA            complement(690..765)
                     /locus_tag="OQM47_003905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQM47_003906"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQM47_003906"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1481852.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM47_003907"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM47_003907"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1481853.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM47_003908"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM47_003908"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1481854.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJVPT010000201         4197 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVPT010000201 DAJVPT010000000
VERSION     DAJVPT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(68..856)
                     /gene="phoH"
                     /locus_tag="OQM47_003909"
     CDS             complement(68..856)
                     /gene="phoH"
                     /locus_tag="OQM47_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1481855.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OQM47_003910"
     CDS             complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OQM47_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1481856.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OQM47_003911"
     CDS             complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OQM47_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1481857.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3919..>4197)
                     /locus_tag="OQM47_003912"
     CDS             complement(3919..>4197)
                     /locus_tag="OQM47_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1481858.1"
                     /translation="SLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFDMSAVLSTHSL
                     FGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA"
BASE COUNT          968 a         1173 c         1018 g         1038 t
ORIGIN      
        1 gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat
       61 aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt acgcaatcct
      121 cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      181 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      241 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      301 gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata taggcgaacg
      361 gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      421 agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      481 caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
      541 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
      601 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc tgcagatagt
      661 gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg cgggccatac
      721 caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
      781 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
      841 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
      901 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
      961 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     1021 agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgcg
     1081 agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca tccgcgatct
     1141 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagaa
     1201 ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca tttcggtgac
     1261 attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc tgtgtgcttt
     1321 ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg gcgcgaacgc
     1381 cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg ataaacctgc
     1441 gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga taatcgttcg
     1501 cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta acgtattcct
     1561 ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt
     1621 ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc cccaacgaat
     1681 aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg ggattcgcca
     1741 gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg taatcaggca
     1801 catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg ccaaaaatcg
     1861 tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga atcaagcgta
     1921 ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc gttacccaca
     1981 ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg tctttgaaac
     2041 ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg gaaataaacc
     2101 cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata tcgcgcagcg
     2161 catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca ccatgacata
     2221 acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt tttggcatct
     2281 gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac gtgatggtga
     2341 gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc aggcgcggat
     2401 ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga gtcaacaagc
     2461 gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc accagcatca
     2521 ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac ggctgtttct
     2581 cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt gcatgagcga
     2641 ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt gacggttcat
     2701 tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag
     2761 ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc ggtcggcatc
     2821 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
     2881 gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt tttgcagttg
     2941 tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
     3001 atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca cttcctcaat
     3061 cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
     3121 ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc ctttggtggt
     3181 gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc
     3241 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
     3301 agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
     3361 ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt
     3421 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
     3481 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
     3541 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
     3601 gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
     3661 ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac
     3721 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc
     3781 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
     3841 acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
     3901 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
     3961 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
     4021 cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
     4081 gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
     4141 aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa acaggct
//
LOCUS       DAJVPT010000202         4183 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVPT010000202 DAJVPT010000000
VERSION     DAJVPT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..276
                     /locus_tag="OQM47_003913"
     CDS             <1..276
                     /locus_tag="OQM47_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1481859.1"
                     /translation="RTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLV
                     ALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            433..1182
                     /locus_tag="OQM47_003914"
     CDS             433..1182
                     /locus_tag="OQM47_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1481860.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1204..1770
                     /gene="yieF"
                     /locus_tag="OQM47_003915"
                     /gene_synonym="chrR"
     CDS             1204..1770
                     /gene="yieF"
                     /locus_tag="OQM47_003915"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1481861.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1824..3161)
                     /gene="adeP"
                     /locus_tag="OQM47_003916"
     CDS             complement(1824..3161)
                     /gene="adeP"
                     /locus_tag="OQM47_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1481862.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3326..3982
                     /gene="yieH"
                     /locus_tag="OQM47_003917"
     CDS             3326..3982
                     /gene="yieH"
                     /locus_tag="OQM47_003917"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1481863.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
BASE COUNT         1135 a         1066 c         1023 g          959 t
ORIGIN      
        1 cgcacgattg ctatgagcct gccggaattc gagcagtcac tgtttatggc agcgcaaccc
       61 gacaatctgc tactggcgac cgcgccgcgc tactgtcagt actacaatca actccatcaa
      121 ctgccgttgg ttgctcttcc tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtt
      181 ccttttaccc tgctgtggca taaacggaac agccataatc cgaagatcgt ctggttacgg
      241 gaaaccatta aaaaccttta cgcgtcgatg gcataaccga atcgtatgaa acgcgaccca
      301 atttcacaat aaaatgtaaa aaagttgtaa taagcttgtc tgaatcgaac ttttagccgc
      361 tttagtctgt ccatcattcc agtaaatgat tactcttgta ttcataatgg accattaagc
      421 atggagcgaa aaatggcgac tcactttgcc cgagggattt taacggaagg acatctgatt
      481 tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa
      541 agccgttttc tggcgtccag aggtttactc gcagaactga tgttcatgct gtatggcatt
      601 ggcgaattgc cggaaatcgt caccctgccg aaaggtaaac cggttttcag tgataaaaat
      661 ttgccttcgt tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa
      721 ggtgaatgtg gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac
      781 gcgcccgata accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat
      841 cctaacgaag cgcgggcgca gctcattacg ctgcgccgaa gcgtgctaaa actaaccggt
      901 gatgttttga atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa
      961 tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc
     1021 gtggcggtca cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc
     1081 tggaaaagcc tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt
     1141 tttgcccagc tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc
     1201 attatgtctg aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt
     1261 aatggcatgg ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg
     1321 ttaccttcca ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt
     1381 ccagcaacgg ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc
     1441 acgccggaat ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc
     1501 cgcctgccgg atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc
     1561 gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca
     1621 atggtgatga acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa
     1681 accggagaag tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt
     1741 ggtgagttta ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc
     1801 gggtttttaa tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac
     1861 agcagcgcaa cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc
     1921 atcacgcagt aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc
     1981 atcatcacgg cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga
     2041 gcaagacttg aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagtct
     2101 ggcaccattc ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc
     2161 actgccgtca gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc
     2221 gtaacggaag aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc
     2281 gcctgcttca tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg
     2341 ccaatcagcg taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct
     2401 gccagcccga ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc
     2461 ggcggcgcag aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc
     2521 gtcaccacga tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg
     2581 atgaaaaagc cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc
     2641 agcgtttccg agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac
     2701 aggccgatac cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag
     2761 taacgaacgc ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc
     2821 ataccgatct gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta
     2881 agccccatcg caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta
     2941 ccgaaagcag caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg
     3001 cccagaattt gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg
     3061 gcaatcactt cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac acgttccagc
     3121 atcctctgac cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg
     3181 agggaaaatc cgtcgctatc gtataccaaa atgcgacaat aggcgcgttt gtgagagact
     3241 tttttattgg atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac
     3301 gttaaactga ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga
     3361 cggtacgctg gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga
     3421 atttggtatt acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta
     3481 cgaaattatc gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga
     3541 acacgtttac cgtgcagaag tcgctcggct gttcgattca gccatcgaag gggctggagc
     3601 gctcctgtca gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat
     3661 gcagcattct atgggcaagc tgaatatgtt gcactacttc ccggataaac tgttcagcgg
     3721 ctacgatatt cagcgctgga agccagaccc ggcgttaatg ttccatgcgg caaaagcgat
     3781 gaatgtaaat gtagaaaact gcattctggt tgatgactca gttgccggtg cgcaatctgg
     3841 tatcgacgca ggtatggaag tgttctactt ctgcgccgac ccgcacaata agccgatcgt
     3901 tcacccgaaa gtcaccacct ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg
     3961 tggttgggat attacggcat agttcttcac actcccttca cttaccccgc ttaaattggc
     4021 gctcaaaggt aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa tacatcacgg
     4081 actttatttt gcggttttag gaccgttaat tggtgttctg tttcttgtcc tctacatatt
     4141 cttcgcaaaa gaaccgctgg ttcttttggt aataatacag gta
//
LOCUS       DAJVPT010000203         4043 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVPT010000203 DAJVPT010000000
VERSION     DAJVPT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(886..1077)
                     /locus_tag="OQM47_003918"
     CDS             complement(886..1077)
                     /locus_tag="OQM47_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1481864.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="OQM47_003919"
     CDS             1142..1465
                     /locus_tag="OQM47_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1481865.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OQM47_003920"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OQM47_003920"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OQM47_003921"
     CDS             2143..2673
                     /locus_tag="OQM47_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1481866.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTHWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OQM47_003922"
     CDS             2680..2913
                     /locus_tag="OQM47_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1481867.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="OQM47_003923"
     CDS             complement(3460..3852)
                     /locus_tag="OQM47_003923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481868.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1208 g          951 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 cactggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAJVPT010000204         3953 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVPT010000204 DAJVPT010000000
VERSION     DAJVPT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1549)
                     /locus_tag="OQM47_003924"
     CDS             complement(<1..1549)
                     /locus_tag="OQM47_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1481869.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISL"
     gene            complement(1634..1969)
                     /locus_tag="OQM47_003925"
     CDS             complement(1634..1969)
                     /locus_tag="OQM47_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1481870.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1969..2325)
                     /locus_tag="OQM47_003926"
     CDS             complement(1969..2325)
                     /locus_tag="OQM47_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1481871.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2325..3821)
                     /locus_tag="OQM47_003927"
     CDS             complement(2325..3821)
                     /locus_tag="OQM47_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1481872.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(3818..>3953)
                     /locus_tag="OQM47_003928"
     CDS             complement(3818..>3953)
                     /locus_tag="OQM47_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1481873.1"
                     /translation="SVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVRVKQDKAKKS
                     "
BASE COUNT          880 a         1209 c          891 g          973 t
ORIGIN      
        1 tcagactgat ctttccgagg ccctttaatt tcagccctgt ttttcctgcc gccgttgtcc
       61 attgattctg ggcgactgtt gccgcacgaa tacttgccac cgccttcaca ccaatataca
      121 gggcagaaac cgttgctatt gtttttactg cctgttccca tccccccatt gctctgacaa
      181 cctgatcaac ctcctgccag acttttttca caaccggacc gaccgtttcc cagttatcaa
      241 ttatgagata tgcaccaccg acgagaagcg caattaatcc ctttgccggc gtcatattca
      301 tcacgctacc catgattttt gttatgcgtg tcaatgtgcc aatcgccacg cccatcgtca
      361 gtaatgccgc accagttttc gcgatggttt ttacgacctc aggattttct ctgacaaacg
      421 tgcgcacttc ttccagaaaa ggcttcatct cttttatgcc ttcattgagt gaaggcagga
      481 aggtttcccc cagcgtggaa gaaatcgcat tgatctggtt ctgcagcagc aacaactgat
      541 tttctgtcgt cgcggcacgg gcggcatatt ctttctgcat tgagccgcca tactgctgcg
      601 catccgccac acgattaaag ttggttcgca acaaatccag attcgtgagc agcggcgcaa
      661 tggcccccag ggattccttc ccgaacagcg ctttcagcac agcagcctgt ttttctttcg
      721 gcacttttgc cagagaatcc agtacgtgca gcatggcccc gcgggcgtct ttctgcatat
      781 ccgccgccag ttttttcggg ctaatgcgca acgagcggag cacttttttc tgcgatttgg
      841 tggcagaatc cctcgctgtc agcgacaaca taaaattctt tatccccgtc gcagcaattt
      901 ctgactccac ccccattccg gcaatggttg cccccattgc ggcaatctct ccggaggcca
      961 cacccgcaac actgcctaaa gggccaatac gggtcacaac atcagaaatc tttttcgcac
     1021 ttgcaggacc ggtattgcca agatagttaa tcttatccgc aagccctgcc acctctccct
     1081 gtgtcagttt aaaggcagtg cgccactgcg ccatcatctg accggattct tctgccgtgg
     1141 tgtcaaaggc cacgcccatc ttcacggcat cgtcagtaaa ctgcatcagc tcgtcacggg
     1201 cgataccagc ctgaccaccc gccgccacaa tttcggcgat accttccgcc gacatgggca
     1261 gttctgttga caggtcgcgc acctgctccg tcattgcctt aaacgcttcc ggcgtatcca
     1321 gaccatccac cactttccgg acatccgcca tttttgattc aagggcaatg gcagatttga
     1381 ccgggagcgc cagcgccccc agtactgcgg ttccggcacc cgctgcgccc agtgacaggc
     1441 tggcaaactc ctttttaaaa cctttcagct ggcgctgcat tcccttcagc ggtgccgata
     1501 ccttatccac ggcagtgatg atcgccttca gctgaaaact gtcagccatg cttcatctcc
     1561 tcgttaatgc ggacggcctc ggcttccagt tctgtaaact gcgaaacagc cacccgtcgc
     1621 agctccagtg gattcagttt ccagaaccag gcaacattgt agagtcgttt ccggaggtgc
     1681 ttcccgtctc cgactgggta aaaaaacgca ggatctgcat gctggtctta aaaatatcca
     1741 gttttgccat ctgcgccgcc gatgaacacg ggatccccgc cagcaacggg atatatttca
     1801 gtgccacctg gctgtccagt ttaatactgc cttcaccgga aataatgaaa ggaaaaccca
     1861 gcgcctcgat ttcgtcatac gtgggctcgc gcaactccag cacatgcagc atttcgttat
     1921 gcgctgtcac cggttttttt aaaactattt ctgtaacgtt cattgccatt actgataccc
     1981 tccctcttcg ccgtggaatt caagatctgc cgtgccttct tcggcattat gattagcctc
     2041 tccgtgcagc catgccgccg aaagcacata caccatgccg tttgccagct cagcggtaat
     2101 cgtcatctgg tctgaggtgg taattttgtc gaccggaaag tttttgggca ctttaaacgt
     2161 gcccttaacg taaggtgacc gccaggtctc cttgtaatcc acatcccctg ccatgccgac
     2221 gatgtcatcc cggacattgg tgttcatcgg cacttcaatg ccacctgtca gcgataactg
     2281 ctgaccatcc actttaaaaa aacaggttcc ggcgatcttt gccattatgc tgactcctct
     2341 gaatactgaa gacggaactg gttaaccacc gcaaagacac gtaactgatt aacataatcc
     2401 ggcgggaaca gcgtattcag tcggttcggg ttatctgcat cacgctcaac tatcagatac
     2461 tgtttaaaca gatcgtaatt ttccacaata ccggcacgct ccatctgacg atatgtcgcc
     2521 agaagttccc ctttgataac ggcaggagtg acaatcgcct gccccggacc aaaacgggta
     2581 ccatcatttg ccagcttgtg acgtccgtac ttgcttgtga tgaccgattt cagtttgcga
     2641 agaacatatg cactggtatg cagagtttca ctgtccagat agctgttgtc tgccacgcca
     2701 tacgcattct ttttgtaggt ggttacggaa cgctggatct gcaacgtgcc gccttccaca
     2761 taagccgtcg ccacgccgtg agataaaagg gtctgctgct ctgtcatgat gaatcgctta
     2821 cctttcggtg ccggaagcat ccccaccagc tcccccgtct gtgtcggacg ggcaggatca
     2881 ttccggataa ataccgcttc acgggcaagg cgactggcaa ccagttcatc gacaggcgac
     2941 tgggtttctt tttcgtaacc ggcaagcgtg atatgttgct gattatgcat atctccggca
     3001 ccaaccagct ctgacagcgt tcccagtttt gcggtataga catgcccgta taactggcgc
     3061 gcataactcc agcgaccgct gctgtcattc atttcggtca tcatcatatt gatggaggcg
     3121 gcatcgttga acggcagacc gataaagtcg aatgcctcat cgcccatagc agcaacagcg
     3181 gcggtaagat caggcgctcc gctaccggca gcaccgactt ccgtcaccac ccgaagccct
     3241 gctggcaaaa tctcaccacc accagaacca taataattca gacagaccgg taactcatta
     3301 ccatacagcc ccttatgacg ggcagtcagc gtcaccacgc ctgcatcaga tgacgccgta
     3361 aacggcaggg tgataacccc gtttaccgct tccttaatcg cggtggcaac cgcagtggca
     3421 tcatcgccat tcaccacagg cacctgtaca cgggagcgcc cgacatacag actcagggtg
     3481 ccgctttcct ctgcttctcc ggcaaccgtc accctgaccg tcgccgccgc ccctctggct
     3541 tccggtactg caataacata cagttcaccg aaaggatctg tctgacggta gacttccacc
     3601 atacgcgcca gctggctccc cgccccacaa atctggcggg cataatctgc cgatggcatc
     3661 agcaccaggc tgttaacctc aatggctgca tcgttgctgg catgcccgat cagtaatgca
     3721 ggcgcgctgg tcaccgctgt atttgccgca gaattgttca tctcggcgta gaacaacggc
     3781 accagcgtat tcgacggaac agtattaaaa cttatcgtca tgatttttta gccttatcct
     3841 gtttaacacg taccacatca cccgccgcta tacggcggaa ccagtagctg ctttcttcca
     3901 catttcgccc ttcggaaggc aaaaggtctc cacgggcagg gtcaggaacc gac
//
LOCUS       DAJVPT010000205         3932 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVPT010000205 DAJVPT010000000
VERSION     DAJVPT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            49..1211
                     /locus_tag="OQM47_003929"
     CDS             join(49..310,310..1211)
                     /locus_tag="OQM47_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCS1481874.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(1243..1788)
                     /locus_tag="OQM47_003930"
                     /pseudo
     CDS             complement(1243..1788)
                     /locus_tag="OQM47_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2450..3439)
                     /locus_tag="OQM47_003931"
     CDS             complement(2450..3439)
                     /locus_tag="OQM47_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481875.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3523..>3932)
                     /locus_tag="OQM47_003932"
     CDS             complement(3523..>3932)
                     /locus_tag="OQM47_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481876.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERRT
                     YGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT         1013 a          943 c         1007 g          969 t
ORIGIN      
        1 agtggacacg cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca
       61 gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag tgaagccctg
      121 aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct gtatgaatca
      181 cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga acgtgaactg
      241 gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga tgaagagctg
      301 gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag tatgtcttta
      361 ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc cgggtggccc
      421 gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt cagcagttcc
      481 gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag cgttacggtg
      541 ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta aaaaccgtgg
      601 cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc agcccggtca
      661 gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat ttttacgcca
      721 gtggcccgaa ccagaagtgg ccaggagaca tcacgtactt acgtacagat gaaggctggc
      781 tgtatctggc agtggtcatt gacctgtggt cacgtgccgt tattggctgg tcaatgtcgc
      841 cacgcatgac ggcgcaactg gcctgcgatg ccctgcagat ggcgctgtgg cggcgtaaga
      901 ggccccggaa cgttatcgtt cacacggacc gtggaggcca gtactgttca gcagattatc
      961 aggcgcaact gaagcggcat aatctgcgtg gaagtatgag cgcaaaaggt tgctgctacg
     1021 ataatgcctg cgtggaaagc ttctttcatt cgctgaaagt ggaatgtatc catggagaac
     1081 actttatcag ccgggaaata atgcgggcaa cggtgtttaa ttatatcgaa tgtgattaca
     1141 atcggtggcg gcggcacagt tggtgtggcg gcctcagtcc ggaacaattt gaaaaccaga
     1201 acctcgctta ggcctgtgtc catattacgt gggtaggatc agaattccat ccagtcgttt
     1261 tgggttgcta atgatgtcct gcatccgctt aatctggtcg ggcgtagaca tataacccca
     1321 cagggtgagc agaacctttt ttgcggctac actatttcct tggtctgata ctaatgccat
     1381 atagccccat aaactatccc tcagccagac agcatcatag tgggtttctg ccgctgtttt
     1441 gtcatcttcg aaagtagaag caagaataaa cgttccccag ggtttctccg tcaacgttaa
     1501 ctttcgtgtg ttttctgtta atgttagcaa tccttgagtt tcgtctttgg tatagtgact
     1561 ctttatttca ttttttaatt tggctgagtg aatcagaaga cgataattag gatacttatc
     1621 tggatttaac gataaataac ttttatctgt tgctgccccc ctggaattaa gaatgttaaa
     1681 tacagattcg cttgtcactt ctgcctctgt cgcctggcat gtgccgcacg aaaatatacc
     1741 agcgagcaga agagttaatg cagatttccc tttcataaaa taatccatca atcgtttgtt
     1801 aaaagcgtag ataaccactt attcaacaac ttgaaaataa ggtattttac ctgagttgtt
     1861 acaaaaggat tgcattgcgt aaacgctttt tatttacaaa aaaatgggga agtgttacag
     1921 cgattgcgaa aagcctgaag tgattgaatt aagataatat agaaaatcag atacagcaaa
     1981 aatggcagat tgatcaggtg aagataaatt ggagcgggcg aagggagcct acgacaaatc
     2041 gaagacaacc agctaaccta ttgtttctta aagacaggtg aatccagttg gttgtagtga
     2101 tagtcacaca tttagagata gaaggtcaag acttgaagca cgtaatattc cctctaaagg
     2161 aatagaactc acggtctgac tacccggccc ttttggtctg gctcaggtca ggctatgggg
     2221 gcttttggct cgctctttat attagatgag gtctcacatt tttcagtcaa aactcatcgt
     2281 ctaatagccc atagccagac caccagtccc agctttagca ggaagaccac gagatagact
     2341 ttgtgtaggt ccatttggcc ctcctacttt ctgtattcac aaactagatt actagtacaa
     2401 aataaaggcc agacatccag ttcggaagtc tgaccaatag tcgttattgt tagttagctt
     2461 ctttagtgaa gctcaggcgt ttgactacag gcaggacagc agtagccgag ccagtaccca
     2521 tcttgatggt cgtagcagct tcagctacaa caaacacctt gccgagaatc tcaaggtcgt
     2581 gagactggga gtctccatca cgtgaaagga ccacagtaat gctctcgcca tcgtgacgga
     2641 agcaaccgaa gctcagagcc gtaaaacgct tagccatcat gaacacgcca cggtcttcac
     2701 cgttgtaata ggcgacgtca gtccccagca tatcgacgat agcgtcttca ccaccgaagc
     2761 cctgcttagc agccagacgg gagagagcct tgaggtgtga ctcatgggcg ataatgccgt
     2821 agtcagaaac ctcagaaccg aaagaagact ccgtgttctc gtacagggca tccatgatag
     2881 cgtcagcctt agcggtcagg ctaccagtca gggccggaag ttctacggtc ttcagcagct
     2941 ttgaggactc cagcacgtca accatcttag agttaatggt acgaatcacg ttatcgcaga
     3001 tgttcagtgc gtcctgctgg agcgtcccct gagtgaaacc agtggtcgac ttctggtccc
     3061 aaagctttgt ttggtgagac acgaggatcg cctgttcaat cggctcaacg gtgatttcag
     3121 tgccagacgg agcggtcaca acttcaggtc gcttaccacc acggacaggg accagcacag
     3181 agcggtcagc cagagtgtga gcaatgattg gagtcggtaa gctgttacgg gtcggcagga
     3241 cttctacagg gaacgggatg ttatcttgag cgtctttaag tacaccgttc agcatagcct
     3301 taacagtctt agctaactgg agggcataga cctgagggtc ggcgttagtc ggcagacctt
     3361 cattgacctg aatgttgtcg gtatcggtga tgcgtactgg ctcaatgcca aactggaaag
     3421 cgtttttagt catattcatt ataattaatc tccttgagtc aaatacttga agtccctaac
     3481 aggcagtcag ggaccgtgtg ttctcagatg agggacgctt gcttacaagc tgtccatcca
     3541 gttaccgcct gactcaaggt cagcgacgat agcggcacgt gagcgaccag cggaatgagt
     3601 agcgctgcgg aggttttcac cataggtccg gcgttcagct ttgacctgtt cgcggcgctg
     3661 tagctctttt tgccattcgt cttcggtcat gaacatttcg cgggtacgct ctttctgaga
     3721 cgctagctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc ggcgttggta
     3781 ggcggtcatc aggtcattac tgaagaagtt accaatggtc acgccagctt cagccagagc
     3841 ttccagtaac tccatcgcct cgcggctggt ggacggtaag cctgacaggt agcccggaat
     3901 gagggtcttg atagcgagcg acaacggata gg
//
LOCUS       DAJVPT010000206         3806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVPT010000206 DAJVPT010000000
VERSION     DAJVPT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            437..2089
                     /locus_tag="OQM47_003933"
     CDS             437..2089
                     /locus_tag="OQM47_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1481877.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OQM47_003934"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OQM47_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OQM47_003935"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OQM47_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OQM47_003936"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OQM47_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJVPT010000207         3763 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVPT010000207 DAJVPT010000000
VERSION     DAJVPT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..600)
                     /locus_tag="OQM47_003937"
     CDS             complement(<1..600)
                     /locus_tag="OQM47_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1481878.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTT"
     gene            complement(755..913)
                     /locus_tag="OQM47_003938"
     CDS             complement(755..913)
                     /locus_tag="OQM47_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1481879.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(984..1202)
                     /locus_tag="OQM47_003939"
                     /pseudo
     CDS             complement(984..1202)
                     /locus_tag="OQM47_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            1397..2938
                     /gene="istA"
                     /locus_tag="OQM47_003940"
     CDS             1397..2938
                     /gene="istA"
                     /locus_tag="OQM47_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCS1481880.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            2953..3699
                     /gene="istB"
                     /locus_tag="OQM47_003941"
     CDS             2953..3699
                     /gene="istB"
                     /locus_tag="OQM47_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCS1481881.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          999 a          929 c          945 g          890 t
ORIGIN      
        1 ggtggtgact ggctgatgtt cgtcaccata acggtaagtc agtgccgcct gtgccagcgc
       61 ctggcgtgcc ggtggcggca ggaccagcga ctgcatggca tcacgctttt cctcaatccg
      121 gtcaaaaacc cccaccactt cgtaagcccc ttcgataact ttctccacca catttccccg
      181 gtgtggaaca cgcacttccc ccagagactg accgcagacg cagccattct ggcagacgaa
      241 tctgaagtaa cccggcagca tctggtagct ggaggtaccg tcatgagagt tgagcagaat
      301 aatttcaggg acatgttctc cgtttatctc tccggcccgc cgcagacgca gcatgtgttt
      361 tgtgtatccc cggcggcccg ggtcgcgcac gcgggtctgg caggcgaaga atggctgaaa
      421 accttcctgc tgcaggcttt ccagtacggt gatggtggga atgtacgcat atcgtttact
      481 gcgggaagta tgccggtctt caccgaaaat actgggaaca tagtgcatca gttcttcgtg
      541 tgtcagcgga cggtcacggc gtatctggtt cgcataacca aaacgactgg ctagtcgcat
      601 aatttgctcc ttatcggtgg ttacgattta ctggtgtaat aaatgaaaaa gctgcgtctc
      661 ccggagaaga cgcggcctga cagatgaaat gaatgacgtt tattgtctga gaagccctta
      721 actggcgagc tgagtattaa gctgtgttcc ggcatcacca gcgcaactga ccttcagcat
      781 tacggataac cagccgggaa tatgttccct ggtcatcttc agtaaacaca ttgcggtaag
      841 ctgttttgac ggcaacagcc tgttcgcgtg agaaagggcc ttcgggcaat aaacgtgatg
      901 tacaacccgg catatctgtt gctccctgaa agtaaaaagc cccggtcatg atgaccgggg
      961 cctgaaggag agtgacctga ttatcaaaaa gtcacattca gcgtggcctg accgttataa
     1021 ccttcagcgc tgctgccgct gacgctgtgg gcataaccgg cctgaacgcc cagggtgata
     1081 ttttcccgga cacgggcttc cagtccggcc tgcaggtcca gtgacgtgcc attccgggac
     1141 ggtgagaacg tcatgttact gccggcggcg gctgtcccca tgctcatgtc accccgggag
     1201 ctgtgcgtat tttcatgaaa ggagatcact caataacttc catcgagatc gggtaataac
     1261 atttgaacag atcgctgaat aacatcgatg gagatcactt ttgactcatt ttgttattca
     1321 gtgatctcca tcaatgttat tggaacttca caggtgtgtt gatctgtatc ttttgccatt
     1381 ccggtaaagg atacctatgc caacagttcc aatttctatg agaaaactta aagaaattct
     1441 taggcttaaa tacggtgttg gactcagcca tcgacaaatt ggtcgtagtc ttgcaatctc
     1501 cccttccgtt gtatccagat atgctaatcg ggcggctcaa cttggcataa agcagtggcc
     1561 cttacctaca ggatgggatg atacaaaact aaaacatgcg ttccttcaga cccaggttaa
     1621 gatgaagaag cactctctgc ctgactgggc tacagtacac cgggaactgc gtaataaatg
     1681 cgtgacgctg cagctactct gggaagaata ctgtgagcgt aatccaggcg gtttttacag
     1741 ctataaccat tactgccgga tgtaccgtga atggctcaaa accacttcac catcaatgcg
     1801 tcaggtacat aaagctggcg aaaaactttt cgttgattac tgtggaccta ccgttggcgt
     1861 taccgaccct gagaccggag aaataagaac tgctcaggtc atcgtagctg ttctcggggc
     1921 atcaagttac acatgggcag aggccacctg gtctcagcag cttgaagact gggtgatgag
     1981 tcatgttcgc tgcttccagt ggttgggtgg cgttcctgaa cttgttgttc cggacaatct
     2041 gaaaagcgcc acatccaggg catgtaagta tgatcctgac gttaacccta cctaccagca
     2101 gatgcttgag cattataatg tcgcagtttt gcctgcgcgg ccacgtaaac cgaaagataa
     2161 agccaaagct gaagttggcg ttcaggttgt tgaacgctgg atcatggccc gaatcaggca
     2221 tgagatcttc tacagccttg catcgcttaa tcagcgcatt cgggagttgc tggaaagact
     2281 gaataacaaa ataatgcaga agttgggtta ttcacgtgca gaactcttca tccagcttga
     2341 taaacccgca ctgaagcctc ttcctgaagc cagttacagt tacaccctgg tgaagaaagt
     2401 cagagttcat gccgattacc acgtggaaat cgacaaacat tactactcgg ttccatgttc
     2461 gctgttaggc cagcaactgg aagcatggat ctccggagaa ctggtaagac tcttcaatca
     2521 ggggcaggag gttgctgtgc acccgcgcaa gcgtacttat ggctacagta cccgcaacga
     2581 gcacatgcct gaagctcatc gacagcatgc cacctggacg ccagagcgtc ttctggaatg
     2641 ggcggggcac ataggcagtg aaactcatag ttatgtgctt catatactga actctcgtcc
     2701 acatccggaa caaagctatc gcttctgcct tggactcctg aaccttcata aaaaatacag
     2761 taaagccaga cttaatgcag catgtgcaag agctctgaaa acaaaggtat ggcgtctgtc
     2821 aggtattaaa tcgatcctgg aaaaaggtct ggataaacaa cctgttcagg atccaaaacc
     2881 agatctgtta tccacgatgg aacacgaaaa cgtacgcggc agtgagtatt accactgata
     2941 cgggatccaa tgatgaatca tctttacgaa caactgaccg cacttaaact caccggcttc
     3001 cgtgatgcgc ttaaaaagca acttgctcag ccgggcacat accaggagct gggcttcgaa
     3061 gaacgcctgt cattactgac agcagaagaa ctaacctgcc gtgaaaacag gaaggcagag
     3121 cgtctgatca aacatgcacg gttcagactt aatgctgagt tatcaaagct ggattatcgt
     3181 aacaatagag ggctggacag ggccctcatc cgttcactca gtcagggaaa ctggttaacc
     3241 ctgaaacaaa atattttact gaccggggcc accggcagcg gtaaaacgtt cctggcatgt
     3301 gcacttggtc ataatgcctg ccgacaggga tacaaggtct actattatcg ccttaaagcg
     3361 ctgatggaac agtgctatca ggggcatgct gatggaagat acagcaaact tttgaccagg
     3421 ctgaataata gcgatctgct gcttctggat gactgggggc tggaacctct ctcatcagaa
     3481 cagcgtagcg acctgctgga aatagtggat ctgatgtacc aacgaggctc aatcatcgta
     3541 gtgagccagt tgccggtgga aaactggtac aaaatgatcg gagactccac acatgcggat
     3601 gccatcctag atcgactggt tcatggcagt atcaagatcg aacttaaagg agaatcaatg
     3661 cggaaaatac aatctccgtt gaccgaagga gatcagtgaa ggtaatttaa aaacggttct
     3721 gtgaaagtga cacgaaccga tctccatcga tgttactcac cga
//
LOCUS       DAJVPT010000208         3697 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVPT010000208 DAJVPT010000000
VERSION     DAJVPT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..183
                     /locus_tag="OQM47_003942"
     CDS             <1..183
                     /locus_tag="OQM47_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1481882.1"
                     /translation="FGDLKFKYRNREFWCRGDYVDTVGKNTAKIQDYIKHQLEEDKMG
                     EQLSIPCPGSPFTDRK"
     gene            complement(301..1245)
                     /gene="hypT"
                     /locus_tag="OQM47_003943"
                     /gene_synonym="qseD"
     CDS             complement(301..1245)
                     /gene="hypT"
                     /locus_tag="OQM47_003943"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1481883.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1590..2309
                     /locus_tag="OQM47_003944"
     CDS             1590..2309
                     /locus_tag="OQM47_003944"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1481884.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2375..3673
                     /locus_tag="OQM47_003945"
     CDS             2375..3673
                     /locus_tag="OQM47_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCS1481885.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT          914 a          822 c          912 g         1049 t
ORIGIN      
        1 tttggtgatt tgaaattcaa atacaggaac agggagttct ggtgcagggg ggactacgtc
       61 gatacagtgg gtaagaacac ggcgaagata caggattaca taaagcacca gcttgaagag
      121 gataaaatgg gagagcagtt atcgattccc tgtccgggca gcccgtttac ggaccgtaag
      181 taacgaagtt ggatgcaaat gtcagatcgt gtgcgcctgt tagggcgcgg ctggtaagag
      241 agccttatag gcgcatttga aaaacctccg gctatgccgg aggatattta ttgcacgtcg
      301 tcaattgcgg cgtgctggct cttccattga tatctgctgg attaaatcgt ctggcatatg
      361 ttcctgtaaa atacgccaga ggttctcggc ggttctattc agtcgggtgt tcattcggta
      421 aatataagcc tgaatgggga ctgtcaattt agcagcatca aggcatatca gacgtttggt
      481 ctgtagttca tcgacgatcg accagatcgg taaccaggca atgccgtatc cgttgagcgc
      541 catattcttt aataactcgc tcatcgaaga gataaatatc gtgcgcccgg taatacctcc
      601 aacctctgcc agatgacgat tgaccaacct gcccatataa gatgtggcgg tatagttgag
      661 caaaggaacc tggggctgag tgatatcgaa tacagggtgc ccctgtgcgt cggcggcaca
      721 taccgggtac aactcagatt caaacagttt caggcagcaa aaaggcgatt gcataagatc
      781 ttcatcgcga aacgaaataa taaagtcact cttgccttcg cgtaacgtat ttacggcctg
      841 gacaacatca atggcttcaa catgatagac aaattctccg ccataggcgg taagtgaatg
      901 caccagtttg ggtaatacgc tcagggatag tgagtgcgcg gctgcaattt taatgttggg
      961 aacgccgagt aaactctggc cgtttaactc ccccaaattg cattcaagct gctgtaataa
     1021 gctgcgagtc tgggaatgaa agagcttgcc ctcttcggtt aattgtaaag gggtggtcgt
     1081 gcgatcgaat aaaacaacgc cgatcgctgc ctccagcgcc ttaattcgtc ggctgaatgc
     1141 gggttgcgaa agattacgct cttttgccgc ctgagaaaaa tggcgacagg cttccagtgt
     1201 caggaaatcg tataaccatt ttgtctcgat attcttcatt accatggcac ctgtattttc
     1261 aatccaaaga cataataaaa catcccggca ataaaaataa tgttctcaat tagcacaacc
     1321 tggcctttat cattcgtttt gtgcataacc ccttcatttc cggtattgac gttgatatca
     1381 catttcgcca aaaaaacgaa atttttccgt cagataacca gaatacatca tcactatggt
     1441 gaataagcgg aatgttccat agctgcatca tgcgtgtctt gcatcgcctt gtacaacctt
     1501 gcatttctca tcgcccattt cgactgttaa aaaagagagc agcagttatt taccgcgtat
     1561 tgtcattaac gttttgagag gcaggtattg tgaaaggttt aattggtgta ctcggtggaa
     1621 tggggccggc agctaccgtc gacctgttta ataaatttgt aacttttacc gctgcacaat
     1681 gtgatcagga acatattcct ttaattattt cttcgatccc ggatattcct gaccgtaccg
     1741 atgcattaat gcatcatggg cgttcaccat tacccgttat gcgtgattat atgcataagc
     1801 tggaagatgc cggggcagaa tgtattgtta ttccctgtaa taccgcgcat tactggttca
     1861 aagaattaaa agatgcttgt catattgata tattaagtat cgtagaaact acgatcaatg
     1921 aagttcgcgc ctgtggtaaa acccgaattg gcttgttagc caccaatgcc acgctatata
     1981 tgggtttata ccaaaaagga attgagtcgc tgggatttac ctgtgtcagc cccgatgccg
     2041 acggtcagaa aaaagtgatg gaaagtattt attccttgaa agctggcaat atagcgcacg
     2101 cacaaaagtt gatgaatgag caggctgaaa tattattttc ccgcggtgca gaaatccttg
     2161 ttcttggttg tacagaaatt ccggttattc tggcgcaagc cgttaaagag cagccttccc
     2221 gctatattga ctcaacggcg tcactcgttc gtgccggaat aaaatggtac gaaaatcgtg
     2281 tcggtaaaca ttatctttta acccaataat catttaaatc gcagccagaa agttattcgc
     2341 ttttaactga attatattta taacggagaa cattatggtt tggctggaaa ttatcgtagt
     2401 acttggtgca atattttttg gtattcgcca ggggggaatc ggtattggtt tatgtggcgg
     2461 gcttgggctt gccattctga ctctgggatt tggtctgcct atggggtcgc cgccagtcga
     2521 cgtgatcctg attattatga ccgttgttgt ggcggcctca gccttacagg ccgcaggcgg
     2581 aatggattat ctggtacgcg tcgctggtaa ttttatgcgg cgtaacccga aatatatcaa
     2641 cataatcgcg ccgattatca cctggtctat gaccattatg gccggtactg ggtttattgt
     2701 tttttccacg ctgccggtga ttgctgaagt ggcgaaagag tccggtatcc gtccttcccg
     2761 cacgcttgcc ggttcggttg tcgcttcgca ggttgcgatt tccggttcgc cgatcagcgc
     2821 cgcaatggcg gctatgctca ctattatgga ggtaaacggt gtcagtttta ttcaagtgat
     2881 gtcggtatgt cttcccacct cttttgctgc cgcgatggtc gccgctttta ttgcttcccg
     2941 acagggatgt gagttgcagg atgacgaggt ctaccttgag cgcctgcaaa aaggactggt
     3001 gcaaaaatac gagaataata gcagcatcaa gccaggtgcg acgctctccg tcggattgtt
     3061 cctgctggcg acggtcgcta ttgttattct tgctgccttc ccccaactgc gtccgggttt
     3121 tgatatcggt aaaccgatgg aaacacgcga tatcattatt atctgtatgc tctctgccgc
     3181 ctgcctgatg gtggtgctgt gtaagacggc gaccgatgcc attattctca cctccacatt
     3241 ccgtgcgggg atgagttcgc tggcggtaat tctcggcatt gtaaccttag gcaccacttt
     3301 tattgatgcg catttaactg agattaaaga tattgctggt gatattttgc aggcgtatcc
     3361 gatgttgttg gcgctggttc tttttggcac ttgcgcgctg ctctactcgc aaggtgcgac
     3421 cacgccgtta attatcccgc ttgccgtcgc gcttgacgtg cccacctggg caattctggc
     3481 gtcctatgtg gccgtcaccg gagtatttgt actgccaacg tatccaacat cactggctgc
     3541 catggaattt gatactaccg gcacaactcg tgtaggcaaa tatgtgctga atcatccatt
     3601 tatgctgcct gggttggggg gaattattgc tggagtggca ttaggctttg tgattgcgcc
     3661 aatgattatt tagcactatc aaacatcagt atcgatg
//
LOCUS       DAJVPT010000209         3694 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVPT010000209 DAJVPT010000000
VERSION     DAJVPT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(109..966)
                     /gene="sitD"
                     /locus_tag="OQM47_003946"
     CDS             complement(109..966)
                     /gene="sitD"
                     /locus_tag="OQM47_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1481886.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OQM47_003947"
     CDS             complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OQM47_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1481887.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OQM47_003948"
     CDS             complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OQM47_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1481888.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OQM47_003949"
     CDS             complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OQM47_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1481889.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT          991 a          945 c          884 g          874 t
ORIGIN      
        1 gattttacgt attttttgca tgatagaatt cgtacctcct cccaacgacc ttccatggct
       61 ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg atgttgtgct aattgggtga
      121 taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg ttgatgctgg taacggtaaa
      181 cgtcatgatg aacacgaccg cgaataagac gacaatcgtc ggagctggtg cgctgtcgat
      241 aaaaaaggac agccagacac cagtcattga cactattacc gatatgccag tcgccagtag
      301 gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg gcgccaggcg caatgagcag
      361 agaaatagag agaatgatac ctactgcttt cagcgtcgcc acaatggtca gggaaaccat
      421 gcacagcagc ccataatgca gccatctcgt atgcaaaccg cttacctgcg cctgttgata
      481 atcaaagctg aacagcaaaa aatcccgcca ttttacgcta ataacaagtg taaccagccc
      541 ggcaataatc accgtctgga ttatgtcgcc gatggttatc ccgagcatgt cgccgaaaag
      601 aatatggtca agatgtacgt ctggtttaat tgcaatatac aagatcagac ctgcggcaaa
      661 catgccagaa aaaacaatcc ccataactgt atcttgcttg attcggctgt tgtctttcag
      721 gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat gcccctgtag ccagaggtaa
      781 ccccagaatc caggtaagca cgatgccagg aaaaaccgca tgactcatcg cgtcacccat
      841 cagtgcccaa cctttcagga ccagaaagac cgacagcagc gcacagggta tggaaactac
      901 cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc agtggctcca gtaataatgc
      961 catcatgagc gatcctccag cgctttatgc gcacgccgac gatttgccaa aagtccatgg
     1021 gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct gcgccaccac gatgattcca
     1081 cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga ggaaactggt tacactgcca
     1141 atagttacag caattatgag caggcgggga aaacgatcgg ttaacaacca tgctgttgca
     1201 ccgggagtca ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg
     1261 gtcgaaaccg acagcagggt gaagaagagg atttttaatc tgcccggatg taatccaata
     1321 gcacgggcgt gattctcgtc gaaaaaggtc accataagat ctttccattt gaaaaaaagc
     1381 acgatgatcg agagaatgcc aatgatagtg agttgcagga tatccgccgg atcgattgcc
     1441 agaatattgc ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa tgacaccata
     1501 aacagcccca ggccaaaaaa ggacgagaag atcaggccga tgatggcatc ttcttttaag
     1561 cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca gtccgccgga aaagaaggct
     1621 cccagagaaa acggaagccc cagcatatat gcccccgcaa cacccgggac aatagagtgc
     1681 gacagcgcat cgccaatcag cgaccagcct tttagcatca gatagcatga caggaaagcg
     1741 cagagaccgc cgaccattgc cgagacccac atcgcattga gcatatattc atagctgaag
     1801 ggttccagta gcacgttcat cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa
     1861 aagggcgttc atcgtcagta ataatgcttt cttccgagcc gttaagtgtg acatggcgca
     1921 gtacgccgct aaaagccagc tccaggttct cggctgtaaa agtggtgtct gtcggcccgc
     1981 ttgccaggac ggtgcctttg accatgaccg tataatcgca aaatgtcgtg actgagccga
     2041 gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg taactccctc aacaggctga
     2101 tgattttcgc ttcggttttc acatcaacgc cggtaaaagg ttcatccagc aggatcacat
     2161 caccctgctg tgcaattgct ctcgccagaa agacgcgttt cttttgtcca ccggaaagct
     2221 cgccgatttg acgatggcga aaatccacca tatcgacacg ttccagcgca tcggtgacaa
     2281 tctgacgatc cctctttttg gctatacgca acatacccat atgcccatag cggcccatca
     2341 tcaccacatc ttccaccagt acaggaaatg accagtcaac ctcttctgac tgagggacgt
     2401 aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat acccagaaca gatatctttc
     2461 cgctggtcag acgcacaaat cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt
     2521 ttaccccaac cagagcggca atagatccac ttggtactgt gaaggatgca tcgcgcagtg
     2581 ctgtgtgccc gttacgccag gtgacagtaa catcattaac gacaatgcct gcagattgca
     2641 tcattatttc tcccttttcc cggctttaat tccctgtacc agcgtactgg tagtaacctt
     2701 aagaaggtcg atatacgtag gtaccgggcc gttttctgtg ctcagggaat cgacatagag
     2761 cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga cgcgctggtt tatcggaaat
     2821 cgtactctcg ctaaagactg ccgggatatg atttttctta actatatcaa caaccttacg
     2881 tacctgctgc ggtgttcctt gttgatcggc attaatcggc cacagataaa gctcttttag
     2941 ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc atcgctgatt
     3001 ctcagggagt tccgtaatct gcttacgcag gggggcaagg gtttgggtaa tcttggcttt
     3061 ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt ttatcaacgc
     3121 atcacgaata ttatcgacgt aaatcagagc attatctggc gacatccatg catggggatt
     3181 aggtttgccc tcatagggtc cttcggtgat ccctactggc gtcacacccg aagagacaat
     3241 tacttctgga accccattga gatgctggta aaagcgttgg aaccacaatt ccagattcat
     3301 accattggcg agaatcagtt gtgccccctg cgcacgttta atatcgccag gggtaggctg
     3361 atactcatga atttctgcac caggcttggt tatggatgag acttctgcag catctccagc
     3421 cacgtttttg gccatatccg cgatgatggt aaatgttgta atgaccttga atttttcagt
     3481 tgcacttgcc tgaaatgcga tcgagcaggt gagggcgagg caccccaaga gcatggttac
     3541 tttttttatt gagtgcataa tttagtacct atagtgagca aaattagaac aaacatagca
     3601 acggctatgt tttatagcat aacctataca aaatgaaaat aattatcatt aacagctttg
     3661 aggcctaaaa cagggattag gttgtttcct gctg
//
LOCUS       DAJVPT010000210         3690 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVPT010000210 DAJVPT010000000
VERSION     DAJVPT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..224
                     /locus_tag="OQM47_003950"
     CDS             <1..224
                     /locus_tag="OQM47_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1481890.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OQM47_003951"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OQM47_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1481891.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OQM47_003952"
     CDS             518..1108
                     /locus_tag="OQM47_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1481892.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OQM47_003953"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OQM47_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1481893.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OQM47_003954"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OQM47_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1481894.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OQM47_003955"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OQM47_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1481895.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OQM47_003956"
     CDS             3457..3648
                     /locus_tag="OQM47_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481896.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          998 a          901 c          928 g          863 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 tacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga
//
LOCUS       DAJVPT010000211         3592 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVPT010000211 DAJVPT010000000
VERSION     DAJVPT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(155..802)
                     /gene="ychE"
                     /locus_tag="OQM47_003957"
     CDS             complement(155..802)
                     /gene="ychE"
                     /locus_tag="OQM47_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1481897.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1279..>3592
                     /gene="adhE"
                     /locus_tag="OQM47_003958"
                     /gene_synonym="adhC"
     CDS             1279..>3592
                     /gene="adhE"
                     /locus_tag="OQM47_003958"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1481898.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQA"
BASE COUNT          993 a          888 c          822 g          889 t
ORIGIN      
        1 tgaggagaag cagttaagga aatccttata actaactgta atttgagaag aatattttta
       61 cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg acgaagtaaa
      121 cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg gggaaaatcc
      181 ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc cccataatac
      241 gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt gccatgcgga
      301 acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca aacagatagc
      361 taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct ggccccgcca
      421 tcaacggcaa cgccaatggc accacaccaa tactttcacg taccgcggtt tctgattttt
      481 cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc gctatcgtca
      541 ccaccaggat gcccccggcg atacggaacg aatcaattga tataccaaaa agttgtagaa
      601 tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg ttggctgtaa
      661 ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata aagacgggaa
      721 taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag taaacgggaa
      781 aatcaaaaag ggtctgaatc acggttcgct ccgaagcaaa agccggataa tgttagccat
      841 aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt cacctcctaa
      901 ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat gctaatgtag
      961 ccaccaaatc atactacaat ttattaactg ttagctataa tggcgaaaag cgatgctgaa
     1021 aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat cagtacccag aagtgagtaa
     1081 tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg tttaaactct
     1141 tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gattttcatt ggttaagcaa
     1201 atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa aaaagtttaa
     1261 cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc actcgtagag
     1321 cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt agacaaaatc
     1381 ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa aatggccgtt
     1441 gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt tgcttctgaa
     1501 tatatctaca acgcctataa agatgaaaaa acctgtggtg ttctgtctga agacgacact
     1561 tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt tccgaccact
     1621 aacccgactt caactgctat cttcaaatcg ctgatcagcc tgaagacccg taacgccatt
     1681 atcttctccc cgcacccacg tgcaaaagat gcaaccaaca aagcggctga tatcgttctg
     1741 caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga tcaaccttct
     1801 gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct cgcgactggt
     1861 ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg tgtaggcgcg
     1921 ggtaacactc cagttgttat tgatgaaact gctgatatca aacgtgcagt tgcatctgta
     1981 ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc tgttgttgtt
     2041 gttgactctg tttatgacgc agtacgtgaa cgtttcgcaa cccacggcgg ctatctgttg
     2101 cagggtaaag agctgaaagc tgttcaggat gttatcctga aaaatggtgc gctgaacgcg
     2161 gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc tgtaccagaa
     2221 aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc gttcgcacat
     2281 gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga cgcggtagaa
     2341 aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct gtacactgac
     2401 caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac agctcgtatc
     2461 ctgattaaca ccccggcttc tcagggtggt atcggtgacc tgtataactt caaactcgca
     2521 ccttccctga ctctgggttg tggttcctgg ggtggtaact ccatctctga aaacgttggt
     2581 ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat gttgtggcac
     2641 aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct ggatgaagtg
     2701 attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt caacaatggt
     2761 tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga agtcttcttc
     2821 gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact ggcaaactcc
     2881 ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc cgcgaagatc
     2941 atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct gcgctttatg
     3001 gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa aatgatcgct
     3061 gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt aactgacgac
     3121 gctactggtc agaaataccc gctggcggac tatgcgctga ccccggatat ggcgattgtc
     3181 gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg tctggacgca
     3241 gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc tgatggtcag
     3301 gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca cgaagggtct
     3361 aaaaatccgg tagcgcgtga acgtgttcac agtgcagcaa ctatcgcggg tatcgcattt
     3421 gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc ccagttccat
     3481 attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta caatgcgaac
     3541 gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc tc
//
LOCUS       DAJVPT010000212         3584 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVPT010000212 DAJVPT010000000
VERSION     DAJVPT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            63..1352
                     /locus_tag="OQM47_003959"
     CDS             63..1352
                     /locus_tag="OQM47_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HCS1481899.1"
                     /translation="MTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGR
                     FGNEYSGWFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSD
                     MYVTTKGFLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKID
                     IALVREDVDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKD
                     NQDNNGYYDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFN
                     GRYYGDHTGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAV
                     VRPAYIWDQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEI
                     RFYATYIKALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            1379..2554
                     /locus_tag="OQM47_003960"
     CDS             1379..2554
                     /locus_tag="OQM47_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS1481900.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            2556..3278
                     /locus_tag="OQM47_003961"
     CDS             2556..3278
                     /locus_tag="OQM47_003961"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1481901.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(3340..>3584)
                     /locus_tag="OQM47_003962"
     CDS             complement(3340..>3584)
                     /locus_tag="OQM47_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1481902.1"
                     /translation="GWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFG
                     IRNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT          961 a          808 c          903 g          912 t
ORIGIN      
        1 atgctgtact ggtcaaaaat gaagagaaaa atgccagtga gacaggctcg atttattctt
       61 ccatgactct gaaagatttc agtaagtttg tgaaagatga aattggcttt agttacaacg
      121 gctattaccg ttctggttgg gggaccgcct ctcatggttc acctaaatca tgggcgattg
      181 gttctctggg ccgctttggt aacgaatact ccggctggtt tgatttgcag ttaaaacaac
      241 gtgtctacaa cgaaaacggc aaacgggttg atgccgttgt gatgatagat ggtaacgttg
      301 gtcagcagta ctctaccggc tggtttggcg ataatgccgg tggcgagaac tttatgcagt
      361 tctccgatat gtacgttacc accaaaggtt tcctgccctt tgcgccagag gctgatttct
      421 gggtgggtaa acacggtgcg ccgaaaattg aaatccagat gctggactgg aaaacgcagc
      481 gtactgatgc tgcagcgggt gtaggtctgg aaaactggaa agtcggtccg ggtaaaattg
      541 atatcgcgct ggttcgcgaa gatgtcgatg attacgatcg cagcctgcaa aacaaacagc
      601 agattaatac ccatactatt gatttacgct ataaagatat cccgttatgg gataaagcga
      661 ccttaatggt gagtggtcgt tatgtcacgg caaacgaaag cgcatcggaa aaagataatc
      721 aggataataa cgggtattat gactggaaag atacctggat gttcggcaca tctttaacgc
      781 agaaatttga taaaggtggc ttcaacgaat tctccttcct ggttgcgaat aactctatcg
      841 ccaggaactt tggccgttat gctggcgcaa gtccatttac cacctttaat ggtcgttatt
      901 atggtgatca caccggcgga acagcagttc gtctgacctc gcagggcgaa gcctatatcg
      961 gcgatcattt tattgtggct aacgcgattg tttactcctt cggtaacaat atttatagct
     1021 acgaaacagg cgcacactct gatttcgaat ctattcgtgc ggttgttcgc ccggcctata
     1081 tttgggacca atataaccag acaggtgttg aactgggcta tttcacccag caaaacaaag
     1141 atgcgaatag taataaattt aatgagtctg gttataaaac cacgctcttc cataccttta
     1201 aagtcaatac cagtatgttg acctcgcgtc tggaaattcg tttctacgcc acgtatatca
     1261 aagccctgga aaacgaactg gatggcttca ccttcgaaga caataaagac gcccagtttg
     1321 ctgtcggtgc ccaggctgaa atctggtggt aatggaactt aatgaaagga atattacgat
     1381 gaatataaaa attgctgctt taacgctggc tattgccagc ggtatttctg ctcagtgggc
     1441 catagcagcg gatatgccag ccagcccggc acccactatc ccggtgaaac agtatgtgac
     1501 tcaggtcaat gccgataaca gcgtgacctt tcgctacttt gcccctgggg caaaaaatgt
     1561 ctctgtagtg gtgggtgttc cggttccgga caatattcac ccgatgacca aagacgaagc
     1621 aggagtctgg tcgtggcgca cacctatcct gaaaggcaac ctgtacgagt attttttcaa
     1681 tgttgatggt gtacgcagca ttgatacagg caccgcaatg actaagcctc agcgccaggt
     1741 taactccagt atgattctgg tgccaggcag ttatctggat acgcgttctg ttgcgcatgg
     1801 tgatttgatc gccataactt accactccaa cgcattgcaa tctgaacgtc agatgtatgt
     1861 ctggaccccg ccaggataca ccggcatggg cgagcctttg ccagtgctct atttctatca
     1921 cggttttggt gataccggac gttccgctat cgatcagggg cgtatcccgc aaatcatgga
     1981 taacctgctg gctgaaggga aaattaaacc gatgctggtg gtgatcccgg ataccgaaac
     2041 cgatgcgaag ggcattattc ccgaagattt cgtgcctcag gaaagacgta aagtctttta
     2101 tccgctgaat gctaaagcgg cagatcgcga actgatgaac gatattatcc cgctgattag
     2161 caagcgtttt agtgtccgta aagatgccga tggtcgcgcg ctggcagggc tttcacaagg
     2221 cgggtaccag gcgctggttt ccggaatgaa tcatctggaa agctttggct ggctggccac
     2281 attcagtggt gttaccacga caaccgtacc ggatgaaggt gtcgcggccc ggctgaacga
     2341 tccggcagct attaaccagc aactacgtaa ttttactgtg gtcgttggtg ataaagatgt
     2401 cgtaaccggc aaggatatcg ccgggctgaa aactgagctt gagcagaaaa aaattaagtt
     2461 tgattaccag gaatatcccg gcctgaacca tgaaatggat gtctggcggc ctgcctatgc
     2521 agccttcgta cagaaattta aaataaggca ttaagatgaa attaatcatt accgaagatt
     2581 accaggaaat gagccgtgtc gcggcacacc atctgcttgg ttatatgtcg aagacgcgtc
     2641 gtgttaacct ggcaattacc gccggtagca cgcccaaagg catgtatgaa tacctcacca
     2701 ccctggttaa aggtaagcct tggtacgata actgctattt ctataatttc gatgaaattc
     2761 cctttcgcgg caaagaggga gaaggcgtaa cgattaccaa tctgcgtaat ctgtttttca
     2821 cccctgcggg gatcaaagaa gagaatatcc agaagctcac tattgataac taccgcgagc
     2881 atgatcagaa actggcgcgt gaaggcggcc tggatttggt ggtgctggga ttaggtgcag
     2941 atggtcattt ttgtggcaat ttgccgaata cgacccattt ccatgagcaa accgtggagt
     3001 tcccgatcca gggagagatg gttgatattg tggcgcatgg tgagttgggc ggcgactttt
     3061 cgttagtgcc tgacagctac gtgacgatgg ggccgaaaag catcatggcg gcgaaaaact
     3121 tgctgataat tgtcagtggc gcaggtaaag cgcaggcact gaaaaatgtg ctgcaaggtc
     3181 cggttaccga agatgttcca gcttccgtgt tgcagctgca tccttcgctg atggtaatcg
     3241 ctgataaagc cgctgccgca gaattagcgt tcggttaagc tgaagaaata aggccggatg
     3301 tatgaattac gctccggcct gttctcatta tttaaataac tacgaaaaat caccccacag
     3361 tctcagcttg ccgcagtgga gacagcggaa gagataaccc tgacaatcac cacccttttg
     3421 caggcattta gctagatcag aatttctaat accgaaattc tcacaatctt cttcaaggtt
     3481 ggcaaattca tcgaggcgat cttttatatc attccagccc acatagccga taaaagcaca
     3541 gaaatcgcca caatgcgcaa gccagaattc ctgttgccat ccat
//
LOCUS       DAJVPT010000213         3554 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVPT010000213 DAJVPT010000000
VERSION     DAJVPT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..156
                     /locus_tag="OQM47_003963"
     CDS             <1..156
                     /locus_tag="OQM47_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481903.1"
                     /translation="NHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFS
                     FAEHGLL"
     gene            219..440
                     /locus_tag="OQM47_003964"
     CDS             219..440
                     /locus_tag="OQM47_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1481904.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            603..977
                     /locus_tag="OQM47_003965"
     CDS             603..977
                     /locus_tag="OQM47_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1481905.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1024..1398
                     /locus_tag="OQM47_003966"
     CDS             1024..1398
                     /locus_tag="OQM47_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1481906.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1395..1886
                     /locus_tag="OQM47_003967"
     CDS             1395..1886
                     /locus_tag="OQM47_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481907.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            1898..2095
                     /locus_tag="OQM47_003968"
     CDS             1898..2095
                     /locus_tag="OQM47_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481908.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2180..2740
                     /locus_tag="OQM47_003969"
                     /pseudo
     CDS             2180..2740
                     /locus_tag="OQM47_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            2812..>3554
                     /locus_tag="OQM47_003970"
     CDS             2812..>3554
                     /locus_tag="OQM47_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCS1481909.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGL"
BASE COUNT          888 a          941 c          865 g          860 t
ORIGIN      
        1 aatcacccgt ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg
       61 gtacaggcac tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
      121 caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg
      181 ttttcacttt tatcttctgt cttcagaggt atcacatcat gagaatcatc acccgtggtg
      241 aagccatgcg tattcaccaa cagcatcctg catcccgtct ttttccgttc tgtaccggta
      301 agtatcgctg gcacggcagt gctgaagcgt atactggtcg tgaagtgcag gatattcccg
      361 gcgttcttgc cgtgtttgca gaacgccgta aggacagttt tggcccgtat gtccggctga
      421 tgagtgtcac cctgaactga tgttccgtca cccgggaaac gagtacgacc agatgacctc
      481 agacgccgct gtggatgagg ccattctcat caatgcatac atcttcaccg aaaccggtca
      541 gcgcgccggc tgacctgtca tcgttaaacc attcttaacc acttttcaga gaggatttta
      601 tcgtgtcaga caaactctcc gggataactc atcccgatga caatcacgac cgcccctggt
      661 gggggcttcc ctgcacagtg aggccctgtt ttggcgctcg tctggtgcaa gagggtaacc
      721 gcctgtatta ccttgccgac cgcgccggta tcagaggccg cttcagcgac gcagatgcat
      781 accatctgga ccaggccttt ccgctgctga tgaaacaact ggaactcatg ctcaccagcg
      841 gtgaactgaa tccccgatat cagcataccg tcacgctgta tgcaaaaggg ctgacctgcg
      901 aagccgacac cctcggctcc tgtggttacg tttatctggc tgtttatccg acaccagcac
      961 ccgcaaccac ctcataaccc cgaagctctc acctttagca ctaccagtta cagagaactt
     1021 aagatgaaaa cattatccga cacacatgta cgggaggtat ctcgctgccc gtctcccgtc
     1081 accatctggc agacactgct catccgactg ctggaccagc attatggcct cacactgaat
     1141 gacacaccgt ttgtcgatga acgtgtgatt gagcagcata ttgaggcagg catttcactg
     1201 tgtgatgcag ttaattttct cgtggaaaaa tacgcgctgg tgcgtaccga ccagccggga
     1261 ttcagcacct gtccccgctc tcagttaata aacagtattg atatcctccg ggctcgccgg
     1321 gcgaccggcc tgatgacccg cgacaattac agaacggtaa ataacattac cctgggtaag
     1381 tatccggagg cgaaatgatg aaactggccc tgacactgga agccgacagc gttaacgtac
     1441 aggcactgaa catagggcgc attgtcgttg acgtcgatgg tattgagctc gctgaactga
     1501 ttaacatggt ctgcgataac ggctactccc ttcgtgttgt tgatgaatct gaccagacct
     1561 cagcagaatg cacgccacca tttgctaccc ttaccggcat acgctgcagt accgcacata
     1621 tcacggaaac ggacaacgcc tggttgtact cgctgtcaca ccagaccagt gacttcggtg
     1681 aatcagaatg gattcatttt acaggtagcg gatatctgtt acgtaccgat gcgtggtcat
     1741 acccggttct gcggcttaaa cgcctggggc tgtcaaaaac gttccgtcgt ctggtcgtca
     1801 cactcatccg gcgttatggc gtcagtctca ttcatctgga tgccagcgct gaatgcctgc
     1861 cgggtttacc cactttcgac tggtaaccag gaacaacatg aaatcattaa ccacggaaac
     1921 agcgctggat attctgattg cgtggctgca ggacaatatc gactgcggat cgggaattat
     1981 ctttgacaac gatgaggata aaacggattc agcagcactg ttgccctgta tcgaacaggc
     2041 ccgggaggat gtccgtaccc tgcgtcatct gcagcttctg caccagaacc ggtgaagctc
     2101 actcaccaga cttcattcct ctgacgccag cctgcacagg gctggcgttt tcatttatct
     2161 gcaaaaagga atatcgatta tgtcagatat cacaatctcc cgtccggaag tggtcaacgg
     2221 gcatacggac gttatctgct caaccagcat tcgccacatt ctggctgtac gaaagagtac
     2281 actgttgcaa atcgacacac ttatccgaca actggctgaa atctcggtgc tgacggaaag
     2341 catcggaggt aaaactgccc cggactgggc catgaaacag gattttcgct gcggctgctg
     2401 gctgatggag aaacccgaaa cagcaatgaa agccattacc cggaatcttg atcgcgaaat
     2461 ctggcgtgac ctgatgcaac gttccgggat gctgtcatta atggatacgc aggcccgtga
     2521 tacatggtac cggtcactgg agtacgataa ttttccggaa atcagcgaag cgaacattct
     2581 gagcacattt gaacaactgc accagaataa ggatgaggtg tttgagcgag gggtgatcaa
     2641 cgtcttcaga gggctgaact ggaattacaa aaccaattgc ccctgtaaat ttggcagtaa
     2701 aattatcgtc aacaatctgg tgagatggga ccggtgggga taatgatatg gtcaccccca
     2761 ccttaacagc agtacgctgt taaggtaaat ccctttaacg gagagaccat catgcagaac
     2821 atcacactta ttggtatcga tctcggaaag cactccttcc acattcattg tcaagacaag
     2881 tctggtaagg ctcttctgcg taaaaaattt acccgtacta aactgatgca gtttctggcg
     2941 acatgtccgc cctcagttgt tgtaatggaa gcctgtgcgg gtgcacactt tatggctcgc
     3001 tgtatctctg attttgggca tgaaactaag ctgatttctc cacagttcgt tcgcccgttt
     3061 gtcaaaagta ataagaatga ttttgttgat gcagaagcca tatgcgaagc ggcttcacga
     3121 ccatcaatgc gttttgtcca gccaagaact gagacccagc aggccatgag ggcgttacac
     3181 cgggtcagag aatcgctcat cagagataag gtgaaaacaa ccaaccagat ccacggtttc
     3241 ttgctggaat ttggaatcag cctgccgata ggtgatgctg taataaaacg gttgtctctc
     3301 gtgttggctg aacacgaaat ccccgaatat ctgagccgcc ttttggtgag gttgcatacc
     3361 cactactttt accttgttga gcaaatagca gagctggaat cagaattaag tcagtcaatc
     3421 aacgctgatg ataccgctca gcgcattatg acaattcctg gtgttggtcc gattactgcc
     3481 agcttactat cgtcgcaact tggtgacggg aaacagttct catgcagcag ggattttgca
     3541 gcttccacag gact
//
LOCUS       DAJVPT010000214         3460 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVPT010000214 DAJVPT010000000
VERSION     DAJVPT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..384
                     /locus_tag="OQM47_003971"
     CDS             <1..384
                     /locus_tag="OQM47_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481910.1"
                     /translation="IKNREIRLEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQ
                     VLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFF
                     LSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(627..1322)
                     /locus_tag="OQM47_003972"
     CDS             complement(627..1322)
                     /locus_tag="OQM47_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1481911.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1315..2742)
                     /locus_tag="OQM47_003973"
     CDS             complement(1315..2742)
                     /locus_tag="OQM47_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1481912.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2753..>3460)
                     /locus_tag="OQM47_003974"
     CDS             complement(2753..>3460)
                     /locus_tag="OQM47_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1481913.1"
                     /translation="FFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQPAIN
                     SGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAEKVAA
                     RMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGLELLT
                     FAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISGRLQR
                     EGQKVKVMHIAEVLMSR"
BASE COUNT          879 a          940 c          757 g          884 t
ORIGIN      
        1 atcaagaaca gagaaataag gctggaatgg tcaggagata agagagattt accaactgct
       61 gaagaggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat
      121 aatggcaggc aagttctcga tgagatacaa acgccttcag caattaacag tgagctttat
      181 acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta
      241 actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga
      301 ttttttctaa gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa
      361 taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc
      421 tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac
      481 tgtaaatatc ctggcattac taatgcctgg atatttacag aagagcagca taaccatgac
      541 ggaacggggt taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag
      601 gtttatgttc agatctcata tctctctcag caatcctcaa taatcagata caccgctttc
      661 accggaccat gaaccccgac gactttgata agctcaatat ccgccgttga gctgggacca
      721 ctgatgatgt taatacatga cggcattcgt tcaccggcct gcgctttctg atgcaatttt
      781 tctgcgagtt gcgctacacg cggcaggatt gtgcttttac gcaggataaa aagagaagat
      841 tccgggagca ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaac cacgcctccc
      901 gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag
      961 atattctcgg cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt
     1021 tcactaatcc ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc
     1081 agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag
     1141 cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta
     1201 agttgggtaa gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca
     1261 agtcgcagcg ggcgacccag tgcctgagca acgttattca aaaattcgct ccgattatcc
     1321 attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc
     1381 ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt
     1441 gccgccattg ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa
     1501 tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt
     1561 gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg
     1621 caccggacac acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa
     1681 atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga
     1741 gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca
     1801 gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc
     1861 gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggccctgt
     1921 cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat
     1981 caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg
     2041 cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga
     2101 accggtctct gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc
     2161 ttcacggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata
     2221 gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac
     2281 cacaacatga gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc
     2341 aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat
     2401 cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg
     2461 ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc
     2521 tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc
     2581 ccgatcgcgc cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc
     2641 aatacgctgc tgcgcgcttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg
     2701 gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc
     2761 tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc
     2821 cactgatgtt cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca
     2881 tcaggtgcac aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga
     2941 acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac
     3001 gcacattttt cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac
     3061 aagatgggtg atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt
     3121 tattaacaat aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg
     3181 cccattcagg ttcatccgcc agatacgtcg ggtaactttt tatggcatag gtgcaagagc
     3241 cagccggtga aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc
     3301 ctggaatcgc ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt
     3361 tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca
     3421 ttcttgattt cagggcgtca ccaatacagg tgacaaagaa
//
LOCUS       DAJVPT010000215         3456 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVPT010000215 DAJVPT010000000
VERSION     DAJVPT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            196..285
                     /gene="kdpF"
                     /locus_tag="OQM47_003975"
     CDS             196..285
                     /gene="kdpF"
                     /locus_tag="OQM47_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1481914.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            285..1958
                     /gene="kdpA"
                     /locus_tag="OQM47_003976"
     CDS             285..1958
                     /gene="kdpA"
                     /locus_tag="OQM47_003976"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1481915.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1981..3456
                     /gene="kdpB"
                     /locus_tag="OQM47_003977"
                     /pseudo
     CDS             1981..3456
                     /gene="kdpB"
                     /locus_tag="OQM47_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
BASE COUNT          694 a          861 c         1011 g          890 t
ORIGIN      
        1 ttgcttattg atggtttatt tacattcaca ctgtaattaa attacacaaa agttaaatta
       61 atactaacca tcagttaaat catggccttt gccattttta tacttttttt acaccccgtc
      121 cgcagatttt tgcgaaatct ttgcagccag aattctactc ttccggtatc acttttaggc
      181 cactggaggt gcactgtgag tgcaggcgtg atagccggcg tgttgctggt gtttttatta
      241 ctgggctatt tggtttatgc cctgatcaat gcggaggcgt tctgatggct gcgcaagggt
      301 tcttactgat tgccacgttt ttactggtat taatggtgct ggcgcgtcct ttaggcagcg
      361 ggctggcgcg gctgattaat gacattcctc ttccaggtac agcgggcgtt gaacgtatac
      421 tctttcgttt acctggcgtc tctgaccatg agatgaactg gaagcaatat ctttgtgcca
      481 ttctcggcct gaacatgctg gggctggcgg tgctgttttt tatgttgctc ggtcagcact
      541 atctgccgct taatccacag cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg
      601 ccgtcagctt tgtcacgaat accaactggc aatcttatag cggtgaaacc acgttgagct
      661 atttcagcca gatggcaggc ttaacggtac aaaactttct ttctgccgcc agcgggattg
      721 cggtgatttt tgccttcatc cgtgcgttta ctcgccagag catgagcaca ctcgggaatg
      781 cctgggtcga tctgctacgc atcacgttat gggtgctagt ccctgtggcg ttgttgatcg
      841 cactgttttt tattcaacaa ggtgcgcagc aaaactttct gccctatcag gcagtgaata
      901 ccgttgaagg agcgcaacag ctgttaccca tgggaccagt agcttctcag gaagcgatca
      961 agatgctcgg tactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa
     1021 acccaaccgc actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc
     1081 tgtgctttgc ctttggtgaa gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg
     1141 cgatgtcagt gatttttgtc atctgcgtag gcgtggttat gtgggcagaa gttcagggta
     1201 atcctcatct gctggcgctc ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc
     1261 gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg
     1321 cggtgattgc gatgcatgat tcgtttaccg ctctcggcgg catggtgccg atgtggctaa
     1381 tgcaaattgg tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt
     1441 ttgtcctgct ggcggtgttt attgccgggc tgatgattgg tcgtacaccg gaatatctgg
     1501 gtaaaaaaat agacgtacgc gagatgaaac tgactgcact ggcgattctg gttactccaa
     1561 cactggtact gatgggcgcg gcgttggcga tgatgaccga cgccggacgt agcgccatgc
     1621 ttaaccctag cccgcatggt tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca
     1681 acaacggcag cgcctttgcc ggattaagcg ccaactctcc gttctggaac tgtttactgg
     1741 cgttctgcat gtttgtcggt cgcttcgggg tgattatccc ggtgatggca attgccggtt
     1801 cgctggtgag taaaaagagc caacccgcca gctccggcac gctgccaacg cacggcccgc
     1861 tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg
     1921 ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat
     1981 atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa
     2041 gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg
     2101 atcggcagtc tgctgaccac ctgtattagc atcgcgatgg caagcggtgc gatgcctggc
     2161 aatgcgctgt ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat
     2221 ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg ccaacagtct taaaggggtg
     2281 aaaaaaactg cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa
     2341 gttcctgccg atcaactgcg taaaggtgac atcgtgctgg tagaagctgg cgatattatc
     2401 ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg
     2461 gaatcggcac cggtgatccg tgaatccggc ggcgattttg cctccgtcac cggcggcacg
     2521 cgtattcttt ctgactggct ggtgattgag tgtagcgtta accccggtga gacatttctg
     2581 gatcggatga tcgcgatggt ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc
     2641 ctgactatcc tgctgattgc tctgaccatc gtctttttac tggcaaccgc cacgctgtgg
     2701 ccgttttccg cgtggggcgg taatgccgtg agtgtaacgg tactggtggc gctgctggtc
     2761 tgtctgatcc caaccactat tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt
     2821 cggatgctgg gcgcgaatgt gattgccacc agcggacgtg cagttgaagc ggcaggtgac
     2881 gttgacgttc tgctactgga taaaaccggc accatcacac tcggtaaccg tcaggcgtcg
     2941 gagtttatcc ccgcgcaggg cgtggatgaa aaaacgctgg ctgacgccgc acaactggct
     3001 tcgctggctg atgaaacgcc ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt
     3061 aacctgcgcg agcgcgatgt gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa
     3121 agccggatga gcgggatcaa catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc
     3181 attcgccgcc atattgaggc taacggcggt cacttccctg ccgatgttga tcaaaaagtc
     3241 gatcaggttg cgcgtcaagg agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg
     3301 ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccggctg
     3361 cgcaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     3421 tagctgcgga agcgggtgtc gatgattttc tcgccg
//
LOCUS       DAJVPT010000216         3236 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVPT010000216 DAJVPT010000000
VERSION     DAJVPT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..2631)
                     /gene="ag43"
                     /locus_tag="OQM47_003978"
     CDS             complement(<1..2631)
                     /gene="ag43"
                     /locus_tag="OQM47_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1481916.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
     gene            complement(3003..>3236)
                     /locus_tag="OQM47_003979"
     CDS             complement(3003..>3236)
                     /locus_tag="OQM47_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1481917.1"
                     /translation="MTALPDHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTA
                     ESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          678 a          969 c          832 g          757 t
ORIGIN      
        1 gctgaaagtg cggataacag aaggctgtac ccaccagttc accggcagtt cactcacacg
       61 gtgttttgta ctgtcgcgca gagtgtcacg ggatgaggtg ccttcaccaa agctcatatc
      121 gttgtggctg cccagacgga aaccggcacg cacatgttgt gcactgccat gcccgaactt
      181 cacataaccg gcgttatcct ggccgtcatc cagggtaagt ccctgccagg tatagtgcag
      241 ttgtggctcc agcatcaggt tgtcagtgat actgaagggc agaccggttt ccagtgagcc
      301 cagccagccc cagccccggg cacggaagtc gttattgtcc gatgacgctt tcatgctgtg
      361 gcgggttccc tgtgccacaa tgtcagccca caggccggag gacgtgtgtg tcagattcag
      421 gtatccgccc aggctgccgg catcatcccg gaccgtgccg gcgcgggaac cgtcatcatc
      481 cttaacatca acggaagaat ggcctgcggc accatacacc cctgtcgtca gagacatacc
      541 ggcaacctct gttctcagca ggtcaccctc cagacggacg aagccatagc tgccgctgct
      601 ttccggcgtg gctccacggg caataccgcc gttgttatcg tgaccgagat gaccgccctg
      661 aatgctgaga cggacgctgt tattttcacc gtttacaccg gtctgatggc tgcgggagcc
      721 tgccagaatc cggtcatagt ccattgcctg tgtcagcatg gatgtataca gggggacttc
      781 agcacgataa gcattttcac tgcgcaggta ccagtcttca tcgctgtcac ggttcagggt
      841 gtagttaaag gcgccggcct gaagcgggcg actcagggca aacgcacctt cttctgtggt
      901 ggcgccattc tgtgcatcca caacccggat accctgtccg gaggttgcca ccccgaggtt
      961 actgtttccg acatttgtaa acgcaagcca ggttttgccg gttgcctgac caccattaat
     1021 caccagctgg tcagaggtat tgctgccatc aaggcgaaca cgcatattga tggtgccacc
     1081 ctgaccggtg aggttactgg tggtcagttt atggaacgtt accggggagt cgccttttgc
     1141 aacagcacgg ctcagcgcgg catccggtgt cgtctgatta tcaaagcgaa tctcccctgc
     1201 gttaacaata tcgccggtac tgaccacatc gccattcagt accatgctgg cacctttact
     1261 gagctgtgtt ctgccactca gacttccccc ggcagccagc gtcagtgttc cacgatgtcc
     1321 gacagtggtg ttgctggcca gtcctccggc attaaccgta aagctgccgc cattttccag
     1381 cagcagtccg ctggcctgac cggatgtgcc atcaatactg aacttaccgc tggcattggt
     1441 cccctcaaca gtggcaccac tgtctgcaat cagggcacca ccggatgtca tggtgacacc
     1501 tgttgcctta ccaccggcag acactgccag ggttccgccg tcatccaccc gtgttttctg
     1561 cgctgaatgg ccttccagta catccaggcg accgccggac tccagaacaa ctccgtcagc
     1621 cttaccgttt tccaccgtga aattgcccag acggttgctg ccaagaaccg ttgccgcagt
     1681 actggtaacc agtgctccac cctgtttcag ggtgacatgt gtggcggtac caccggcgtt
     1741 cacctgcagt ttgcctttct ggttaacggt ggtgaaatcc gccagaccac cttccttgat
     1801 aatctgccag ccgtcactgt ttacaacagt gccagacgcc gtaccgccgt tgtgcacata
     1861 ctggtaaccg ccattcagtg tggtatccag cgcatgcccg tgtaccgtct ggtcgccgcc
     1921 ggcataaacc acagtggtat ttgctgttcc ttcagcagcc acaatctgac gaccattttc
     1981 attgatagtg gtacgtacgg catttccgcg aacaaactgc ccggtatcac cattttctgc
     2041 atctggtccc ccttccgcgc cggtattcac aaccgtgtcg gttgccattg cgccggattt
     2101 gacggtctgc cagcccttct cattaatgac ggtacccgtt gcaatcccgc cttcatgtac
     2161 ccactgctca ccgccgttca gagtggtatt cactgcctgc ccctgaaggc tctgtccgcc
     2221 tccggcactg ataaccgtgt ctgaaacgct tcctccggca ttcactctct gaagaccacc
     2281 accggtgaca gtagtgttgt tggcgatacc gccattttgt atccattgcc cgccggtatt
     2341 ggcctcgtta tccggcccat actccagacc gctactgatg gtcattccgt tggccgtacc
     2401 gagaacaatc tggttgtcat gatttgtcag tgttccgccg ttcacggttt ctcccgcctg
     2461 tacaatcgtg tcagcagcca gtgccgggac tgatgtgaca gcagcaagag acagcacaac
     2521 cgccacaccg gcgcgttttc cccgtgagcg ggccagttcg gaagccacca ccagggtgcc
     2581 cgtaatgtga ttccatacca gcctgtagct ggtgttcaga tgtcgtttca tcagcttttc
     2641 cttacagagg gtgaataaaa aagccggtac ccacaaaagt ggataccggc aggtacaaaa
     2701 cagcatggtc agataaagtg gtttttcccg gacataaatg cttcccctct ctcatccgcc
     2761 agagaagtat cagtccgttg tgtgcaattc cgttctgcat gatgaagaga ttcagctgcc
     2821 ttttcgcaga ccattaacca ctataaacga tcgatagcaa cgatcgatat ctgttttatc
     2881 gatagtttta ttctattaac gacaaggcgg tcacgcaaga gaaacaggag gagtatgagg
     2941 ggtttgtgtc atgttttcag ttggttaacg taatgttatc aggcatttcc ctggcagact
     3001 gttcagaaga aaatccagtt ccatacagcg cgggcaacag agaccaccgt gtcacggacg
     3061 gcacgcagaa ccgtacgggc aacagaggca acacagacgc tctccgccgt gtcaaatatc
     3121 cggtccaccg caccggtaaa ctgttcacgg gcctgacccc ggacagattc cgtgcgcagc
     3181 tcgtcctgca ggcgggtcat caggggactg gcggcatggt cgggaagtgc tgtcat
//
LOCUS       DAJVPT010000217         3179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVPT010000217 DAJVPT010000000
VERSION     DAJVPT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OQM47_003980"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OQM47_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1481918.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OQM47_003981"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OQM47_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJVPT010000218         3160 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVPT010000218 DAJVPT010000000
VERSION     DAJVPT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..1911)
                     /gene="flhA"
                     /locus_tag="OQM47_003982"
                     /pseudo
     CDS             complement(1..1911)
                     /gene="flhA"
                     /locus_tag="OQM47_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1904..3051)
                     /gene="flhB"
                     /locus_tag="OQM47_003983"
                     /pseudo
     CDS             complement(1904..3051)
                     /gene="flhB"
                     /locus_tag="OQM47_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          766 a          935 c          786 g          673 t
ORIGIN      
        1 gggatagagc ttcctgagtt tgcgccagta aacgatccgc cagccctggc tccagtcctc
       61 ccccgccctg cagcgcctgt agtaacaaac gttccagcgg tgtatcgagg ccaataacat
      121 ggacttcatc tttgccagga aaccactgct gggtaatcgc ccgtcccaac gccacgcgca
      181 cgacggcgct taattcatgt ggatcgcttt ggatgggcgc atgttccgcc agcgtttcga
      241 gaatggtgcg catatcgcga atcggtactt tttcatcgag gagattttgc agcactttat
      301 gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc cgtcagcttt ggcatctcct
      361 gggcgacgcg atccaacagc tgttgcgcct cctgacgacc aaacagctct gcggcatgct
      421 ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc actgtgtacc
      481 cctgaatctg cgcctgttct tttagcgcac tttcaatcca gatagcattc aggccaaatg
      541 ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc ggttccaggg ttaatcgcca
      601 gccagcgccc cggataagca tcaccactgc caatctccac gcctttcatc aaaatgcgat
      661 agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac cactggcggc agaaatccca
      721 tctcctgggc aaatttcttg cggatactgc gtatacggcc caacaactca ccatcctgct
      781 ggaaatcgac catcgggatc agtcgataac ccacttccat tcccagagaa tcttccagtt
      841 gtacatcgtt ccacgtcgct tcgacaacgg tattattctc tgccattttt accggtttgg
      901 gttcggcagg cgctttttgt tcgcgtccgc gtatccacca ggccagcccc agcaatccgg
      961 cagtgaacag caaaaatacc aggttcggca ttccaggcac caggccgagt aaaccgagca
     1021 cggcggcgct taacaacata acgcttgggt tactgaaaag ctgattcacc atctgctcgc
     1081 caacatcctg atcggtgctg acacgcgtaa cgatgccccc cgcggcggta gaaatcacca
     1141 gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa ctttccgccg
     1201 cgtgtcccat gctcatgcca gttgcagcac gccgaccagc aacccgccga caacgttaat
     1261 gaccatgatg aggatcccgg caatggcatc gccgcgaaca aacttacttg ccccgtccat
     1321 tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cctcatcttc
     1381 accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg gcataccatc
     1441 gagaacaaag cgcgcaccca cttctgctat acgcccggca cctttggtaa tgaccataaa
     1501 gttgatgatc acgagaatga caaacaccac gataccgata gcgaaattgc caccaacgag
     1561 gaagtgaccg aacgcttcga ccaccttccc agccgccgcc gcgccggtat gcccttccat
     1621 caaaatgata cgggttgaag ccacgttaag tgccagacgc aacaacatag taaacaacag
     1681 aatggtcgga aacgcagcaa actcaagcgt gcgctgggta aacatcgcca ccagcaacac
     1741 catgatcgac aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg cgggcagtgg
     1801 cagcaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccatta
     1861 tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca tgggtcggtt
     1921 tctcgttaat aaaatccagg gctgccggca ccggaagatg agtaggtgta cagggcgctg
     1981 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     2041 ggcgtacagt tgacccggga tttgttgacc aatctccgca tgtcgataca gcgctcgcgc
     2101 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     2161 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     2221 ctgcaacgct accgaatagt gggtcgggtt attgacaatg acatccgctt tcggcacatc
     2281 ggccatcatc cgacgccgtg cggcagctcg ctgcatctga cagatccgtc ctttaacatg
     2341 ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgcgaca ttcgcagctt
     2401 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accattggaa tgacaccaag
     2461 caccaccagc agtgcgcata gccctaccaa atccatcgca ttacccatgg cggtaatcgg
     2521 tgactcggcc attaagcgca tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac
     2581 gctgccaacc aggatggttt tcaaaattgc cttaagcaac tccgcgctag tctgtgccga
     2641 gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     2701 gccgctaaat accagccccc ccagcataac cggagaaatg agcgccacca gcaccacgcc
     2761 gctaatcagc ggtagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     2821 gatcagattc gggtctttga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     2881 cgacaatcga cgggccagcg acacaccgcc aaaccagata acactaacgc ccaccagcaa
     2941 aatcagcagt gaggtcagtt cacgggaacg cgggatttgc ccctcttccc gcgctttttc
     3001 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     3061 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttaagta
     3121 aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
//
LOCUS       DAJVPT010000219         3145 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVPT010000219 DAJVPT010000000
VERSION     DAJVPT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..752)
                     /gene="ipaH9.8"
                     /locus_tag="OQM47_003984"
     CDS             complement(<1..752)
                     /gene="ipaH9.8"
                     /locus_tag="OQM47_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1481919.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQQNTL"
     gene            complement(1014..1109)
                     /locus_tag="OQM47_003985"
                     /pseudo
     CDS             complement(1014..1109)
                     /locus_tag="OQM47_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1145..1450)
                     /gene="ccdB"
                     /locus_tag="OQM47_003986"
     CDS             complement(1145..1450)
                     /gene="ccdB"
                     /locus_tag="OQM47_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1481920.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1452..1670)
                     /gene="ccdA"
                     /locus_tag="OQM47_003987"
     CDS             complement(1452..1670)
                     /gene="ccdA"
                     /locus_tag="OQM47_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1481921.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2206..3145)
                     /locus_tag="OQM47_003988"
                     /pseudo
     CDS             complement(2206..3145)
                     /locus_tag="OQM47_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          780 a          759 c          764 g          842 t
ORIGIN      
        1 tggagtgtat tctgttgtcc gtcactcatg gagaagtaaa tccgtgggcc gtggtagtcc
       61 ggcgaagagg ttaatctttg cagggcttgc agagcatggg atgataatgg gttatcacta
      121 atatgtattg aacattcatt cctcagatta agaatgcttt ccgggatatg acttatctga
      181 tttctatcaa aaaaatactc tctgacaacg ggattatttc cctcagaaaa tgctggtagc
      241 tcagtgagga aattacgggt cgctctgaga tttttaagag cctgtgggag cgatggtaag
      301 gagactattt cgttatagct gatattcata gtcaataacg aatcgggtaa agctggcagg
      361 ttttccagct cattgtgttg tacctgaagt gactccagcg caggaggtag cacgggcaat
      421 tctgataatt tattgctggc ggaatataat ttttttagcg taacaggcag ttcaggtagg
      481 ttagttaatt gattatatga tacattgagc agcgttatct gagctggtaa gttgtcaggt
      541 agcgaggaca gatttaaacg gtccagtcga agttcatcgg aattattgat aagacattct
      601 ttaagtcggg agacagcctc atcacgctct tcaccgggga gcgcttgttt ttcccattta
      661 tcccatgctg aaaagtaatc agcatatgta ccggaaatag tgttataaaa agaattttgg
      721 ggcaatgaaa agttattatt tatcggtaac atgatgggca tccagttaaa gccggtggac
      781 aaatggcttt gttaaaagaa ataagtagga gtttttatga agttcattcc atgaaatgaa
      841 cttcagcact tttttattat aataaggata aacaaaattg gctgtaaaaa aacgatactg
      901 tttcagttaa ccgtgaatgg cctgtttgca ccgtattgtt cttgtttttc ggttagttaa
      961 acatcgtact tcatatttga acggtaagcg gctcgtcaga accgtattga tatttactga
     1021 gaggtcagat caactttcca aggcaacaga tcgcgtaccc ggtttgccgg ccagtcctgg
     1081 atatgctcaa tgacgtaacg cagccatttt gtcaacgcca cgatttttga ccctagcctg
     1141 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     1201 tgagatcagc cacttcttcc ccgataacga agatcggcac actggccata tcagtggtca
     1261 tcatgcgcca gctttcatcc ccgacatgca ccaccgggta aagctcacgg gagactttat
     1321 ctgacagcag gcgggcactg gccaggggga tcaccatccg tcgtccgggc gtgtcaataa
     1381 tgtcactctg tacatccaca aacagacgat aacgactctc tcttttatag gcgtaaacct
     1441 taaactgcat ttcaccagtc cctgttctca tcggcaaaag agccgttcat ttcaataaaa
     1501 cgggcgatct cagccatccc ctgctgattt tcagcctgcc agcgttccgc acgcagacgg
     1561 cgggcctctt tttgcatggc cgtatttacc aggcctgaga tattcacgtt tgcggattta
     1621 agtaactgat agctgtcact gtctatagtg acagtaatac gctgcttcat gataagccct
     1681 caaaaacata cttatgatat gtatatgagt atatattaat ggtacaaacc atcaatatcc
     1741 ataaaaagta caccattaat tcgctataag tttttaatac aattttttct gctgcgattt
     1801 ttttatacta taattagcat acttataaca ccgtaagtaa attacatgaa agcaggcaca
     1861 gcgtgatccg atttttctgc gtataacgat tttccggaag tgtaatacgg ttgcgccccg
     1921 ggccgggcac ccttttctgt tgttgtgtac gggcccacag agaatggtac cggaggcggg
     1981 ctcagaagta ctgagtcggg tgatcgcgtc aggccgcgaa gtactgacac gggtattggt
     2041 gactggtgat gaagtactgc cccgggtatg atttcaggct gattcaggtt ctgattctct
     2101 gtcagtccgc gtggttgctg gcctggctga tttcctgttg tcaggcgttc aacaaccgac
     2161 tgcaggttat agcccggttc acgaacacct gacggcaggt atcactcacc cggtgaaggt
     2221 aaatccctca agaggcagca actctgccag tcgctcctcc ggccattccg gcagacgcgt
     2281 caggacgtcc gtcagccagg catgtggctc cagaccatta cgttttgctg tttccagaag
     2341 gctcattatc tgtgccgccc ggtttccggc catcagtgaa ccggcgaaca gccacgcttt
     2401 ccgtcccata acaaccgggc gaatggcacg ctcgcagata ttgttgtcca ggggcacagc
     2461 tccgtcaccc aggaagcagc tcagttcatc gcgccggttc agaatatagt taatcgcctt
     2521 atttaatggc ccgtcgggcg gacagcacgg ctcctgctct tcaagccagg caaacaggtc
     2581 attcactatc ggccgggagt accgttatcg ccactgccgt attttttcca cagggcgctc
     2641 acggatgagt ttctcaatgc ggtaaagacc cgcgattttt ctcagctcca cgcctgcacg
     2701 gggctccctg ctggccttat acagatttgc gaagccgcgc cctgcgtgac tccagcaaca
     2761 ggctgatgtg atgccggggt gattatccgc cagagatttg tacacgctgt atccgtcact
     2821 caccagcgta ctgccacacc agcactgcag ccaggtctca gggtatctga gggcccgtcc
     2881 ggtctgcgag tcaaaacaga cgaccggcgg accgcttctt tcaccgctga cgtaagcaca
     2941 cagccagccg gagcaggatt taccgccttt ccgggtatca aggagcctca gggacgtttc
     3001 atctgcatga ataacatccc gtgtcagaag ctcatggcgt agcagtttcg ccagcggggc
     3061 cagtgcagct ccggcaacac cgaccatatc cgccattgta ctgacgggga gctccacacc
     3121 cattcgggag aaaatgtgct gctgg
//
LOCUS       DAJVPT010000220         3041 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVPT010000220 DAJVPT010000000
VERSION     DAJVPT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..548
                     /locus_tag="OQM47_003989"
     CDS             <1..548
                     /locus_tag="OQM47_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1481922.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
     gene            960..1328
                     /locus_tag="OQM47_003990"
     CDS             960..1328
                     /locus_tag="OQM47_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481923.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            1759..2274
                     /locus_tag="OQM47_003991"
     CDS             1759..2274
                     /locus_tag="OQM47_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481924.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            2264..2764
                     /locus_tag="OQM47_003992"
     CDS             2264..2764
                     /locus_tag="OQM47_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481925.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
BASE COUNT          727 a          794 c          767 g          753 t
ORIGIN      
        1 agtatcagtt cacacgacac acggctcaca cgacctctga ggcccgtaga tgggcgtctc
       61 tggtgtctgt ggtcactggc gctcggtctg ctgaggtctg ccacctgact aaacgtgaca
      121 ttgtaacctt ggacaatgga ctggtctgta tcgacatcaa tgaagatggg gacgggaagt
      181 ctgtgaagaa caaacattca gtccgacttg tgccgttaac cgatggcgct tatgggtttg
      241 acctgacgtc cttcctgtcg tgggtagaca tgcagcctga tgagggtcct ctcttcggga
      301 tgactccgag cgcctactct agctggttta actcaagggt cctgactgag gccctaggag
      361 actctcagga tgtctctctc cacagtctga gacattggct ggcgacccga atgaaagagc
      421 gtggagtcaa tctggtggac gctcaaggca tcttaggtca cagcagtcag tcaattacgt
      481 atgacctgta cgggaaagga cacgctgtgg gtaggctggc tgatgtgctt aagacagctt
      541 tactttaagt agggagaccc aaggtggaga ccatatacgg cttgatggtt gagaccttgg
      601 gtcactgaag tctgactgaa gttagggagg gtgataataa tcataatccc tcctgactat
      661 aggcccactg gtccgccttc ggctgtccga ctctaagtct gtctgtggtc ttatttaaac
      721 tgcgtcctaa actccagtcc ctccaaattc ctatggtctc ctcttgacat tcctgtttaa
      781 ctgtgagtcg cctctggtct ctctttatat atacttcctt aattcctgag gcgtctttag
      841 tccttgacat tcggttcaac ctgtgagtca catcctgtcg cgctactgta tgcatcacca
      901 gtgatggact tgattatcac tataggggaa ccaactaatt caactacagg agatactcta
      961 tggcacgacc aatcgcaact cacgacaaca cgttcaccaa agcctacttg cagcagcact
     1021 gcggggacct gctgtcattc gatggtcagg gcgacctgag tggttggctg gatgatgtcc
     1081 ttactggtgc tggtcgcctg aacgaatcaa tggcaagcaa taccaagcct gtcagcccat
     1141 acttgattct gacccaacta ctaacgcacg acacattaac tgtaagcgct gtgcaggagt
     1201 ccctgagccg taagcgtgtt gccttgggtg aacctatggt ttcaactcgc tacgctcgct
     1261 acgtctatgc taccgtcgtc agcgccagta aatccgtcca gtaccacgcc agtaaagctg
     1321 gttcatgatt acggacccta aactatcact atagggagac cagcctccgc ctctgagtct
     1381 gatgcgtcag aacatgcgag catctggtca accgacatta agtatctctc ctaagtaatc
     1441 aaggcccgtg accgcctctg acagacctcc tgtatcgacc tccagctcgt tagcgttctt
     1501 tagtcgtctc ggttagtagc tcgtctactc actacattgg cttaagtcta tcaccgctca
     1561 ccatctggag tccttacagc tcgtcctgac atgcggtcgc acctttatac gtacatggag
     1621 aacatggtca ctccagaccg tcaacattta accattcact ttcgttaagc actttctgat
     1681 gggtccgttt aggatgcgtc tgaatggtgc ggactccatg tggagacaga aagtgcttta
     1741 ccaaaggagc atctttgtat gaataccgaa ttaccagtaa ctaaccgcca gttcttaatc
     1801 atcctgctga ccaatgcaga gtccagtgtc gattgctgtc gctataactt agacctgctg
     1861 aagttatccc gatggtatgc ccaggcggaa gactgggact atgagaaaat ccgggagatg
     1921 tacgcggagc ctatccagtc atacctccag acacacaccg aggaagagct gaagcgtggc
     1981 ctgcaggaca tcttctattt caacaatcct tacacctgtg agggtgactc agaagagatg
     2041 gattggatag cgtcacgctc tgatgccgac ttgaagcaca tctctaacgc gatgatgcag
     2101 gtggagagca aatggtcccg ccgccttcag gtcctaactg aagattacga aaacggcacc
     2161 tttgataagc tggtcccacc tgatgccgaa cgccttcagg cgaaagctga ccaagaacag
     2221 cgcttcaagt cctttgccga agctctggcg ggaggtgcct cttatgactt ctgacaataa
     2281 gcaggaatac accattcata tcttcggtca ggacatcacc agtcaggacg gattgtttcg
     2341 actggatgag atacgccggg taggcattca gattggtaag gtagaagata accgtagtac
     2401 cgaggttgct cgcttcatga ggaccaaggc tggtagaagt ctaaccaacc cagaaaacgg
     2461 gtttgttaaa aagattaaca tgggtcactt agggacctcg tggctggctg acccagtagc
     2521 cgctatagag ttcggtcggt ggttggacct tgggtacgga ctggcagtaa cccgagcctt
     2581 cgtgtctatg acctccagcg ccaccgtgca gtctgctgta gcgtctgctg aggacatcaa
     2641 gccggagacc aaggaattca tccagcgtca tggcacaggc ctctacatcg ccggacgtga
     2701 agtctcacag gctgagtatt cagactatca agctgtgacc gctggtcgct gtgagtggag
     2761 gtaagtaatc atggctatga ttgaccctcg tacacctatc ggcaaagcga cccttcgtta
     2821 ccgtggcctg cccacacgtc atctgctgtc tctgctgaga cttggagtcg aagaccctga
     2881 gcgcccttac tacagtcgag acgagctgat agctatgctg gtggaccgtg atttagacaa
     2941 tcagttacgc cgagcctttg ctaagcagtc ctgaccatac caaacctaat tttaaacctt
     3001 ttgagatgac cgatgcgggg agacccgtag gcgctactgc g
//
LOCUS       DAJVPT010000221         2939 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVPT010000221 DAJVPT010000000
VERSION     DAJVPT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..2743
                     /locus_tag="OQM47_003993"
     rRNA            <1..2743
                     /locus_tag="OQM47_003993"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2839..>2939
                     /gene="rrf"
                     /locus_tag="OQM47_003994"
     rRNA            2839..>2939
                     /gene="rrf"
                     /locus_tag="OQM47_003994"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          771 a          651 c          923 g          594 t
ORIGIN      
        1 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
       61 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      121 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      181 acaaaaatgc acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      241 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      301 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      361 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      421 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      481 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      541 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      601 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      661 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      721 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
      781 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
      841 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
      901 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
      961 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
     1021 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1081 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1141 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1201 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1261 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1321 ggttttccag gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc
     1381 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1441 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1501 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg
     1561 aagcgacttg ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1621 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1681 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1741 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     1801 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     1861 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     1921 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     1981 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2041 tggacccgtg atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2101 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2161 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2221 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2281 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2341 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2401 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2461 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2521 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2581 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
     2641 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2701 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt
     2761 ttggcggatt gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2821 ctgataaaac agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg
     2881 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagt
//
LOCUS       DAJVPT010000222         2918 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVPT010000222 DAJVPT010000000
VERSION     DAJVPT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..843
                     /gene="ynaI"
                     /locus_tag="OQM47_003995"
     CDS             <1..843
                     /gene="ynaI"
                     /locus_tag="OQM47_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1481926.1"
                     /translation="VIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIK
                     KGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS
                     NFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSS
                     ISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQF
                     ADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPP
                     EQGR"
     gene            complement(894..2507)
                     /gene="mppA"
                     /locus_tag="OQM47_003996"
     CDS             complement(894..2507)
                     /gene="mppA"
                     /locus_tag="OQM47_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1481927.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2844..>2918)
                     /locus_tag="OQM47_003997"
     CDS             complement(2844..>2918)
                     /locus_tag="OQM47_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1481928.1"
                     /translation="YPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          770 a          682 c          644 g          822 t
ORIGIN      
        1 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
       61 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      121 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      181 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      241 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      301 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      361 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      421 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      481 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      541 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      601 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      661 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      721 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
      781 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acaggggcgc
      841 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
      901 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
      961 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1021 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1081 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1141 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1201 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1261 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1321 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1381 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1441 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1501 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1561 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1621 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1681 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1741 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     1801 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     1861 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     1921 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     1981 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2041 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2101 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2161 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2221 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2281 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2341 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2401 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2461 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2521 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2581 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2641 ttagtgtaaa taatagacgc caaaatgtgg caaagcacat agaacaccga acactacgtt
     2701 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     2761 gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag agaagaatta
     2821 aattaatgta tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat
     2881 aatgagaatg ccgagccagc atgtcgacgg tgtggata
//
LOCUS       DAJVPT010000223         2748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVPT010000223 DAJVPT010000000
VERSION     DAJVPT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1354
                     /locus_tag="OQM47_003998"
     CDS             <1..1354
                     /locus_tag="OQM47_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1481929.1"
                     /translation="AVIGAFFLRGADPEVMDILATLPADVFSVRAYQDIYTGICRQAR
                     VSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEAL
                     QKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEK
                     SRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTME
                     MANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTGRNIWMVDQANLAI
                     DEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPL
                     IALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQARGIAEIN
                     ITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF
                     "
     gene            1351..1608
                     /locus_tag="OQM47_003999"
     CDS             1351..1608
                     /locus_tag="OQM47_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481930.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            1703..1903
                     /locus_tag="OQM47_004000"
     CDS             1703..1903
                     /locus_tag="OQM47_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1481931.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            1896..2276
                     /locus_tag="OQM47_004001"
     CDS             1896..2276
                     /locus_tag="OQM47_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1481932.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            2476..2552
                     /locus_tag="OQM47_004002"
     tRNA            2476..2552
                     /locus_tag="OQM47_004002"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2510..2512,aa:Ile,seq:cat)
     gene            2554..2630
                     /locus_tag="OQM47_004003"
     tRNA            2554..2630
                     /locus_tag="OQM47_004003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2588..2590,aa:Arg,seq:tct)
     gene            2633..2708
                     /locus_tag="OQM47_004004"
     tRNA            2633..2708
                     /locus_tag="OQM47_004004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2666..2668,aa:Thr,seq:tgt)
BASE COUNT          718 a          608 c          793 g          629 t
ORIGIN      
        1 cgctgtcatc ggcgcgttct ttctgcgcgg agcagatcct gaagtgatgg atattctggc
       61 cacactgcca gcggacgttt tttctgtacg agcgtatcag gatatctata caggcatctg
      121 cagacaggcc cgtgtttcag gagtgattga ccccgtgctg ttgtgtaatg agatgccgga
      181 acttgccccg gtgattactg ataccgggcg taaaacctgg gtgaagtctt cactggagca
      241 ctatgttgca gcgttgcggc gcaatgccgc actgcgcgat gcagaaaaaa cactgaatga
      301 ggcgctgcag aaattacgtg atgcgcatac ctgtgaagca gctgaagatg ccctgaagga
      361 tgcgcagaac atgatggtca cactgtcgac aggaaagggc gtcattcagc cggtacatat
      421 tgatgatgtg cttccggaag tggttgagcg tgttgaatgc cggaatcagg ggctggagaa
      481 atccaggacg ttgatgaccg gtattgatga actggacgca aaaacaggcg gtatggagcc
      541 cggagacctg gtattcattg ccgcccgtcc ttcgatgggg aaaaccgaac ttgcgctgga
      601 catcatcgac aaggtgactg agcaggggca tggtgtgctt ctgttcacca tggagatggc
      661 gaacatccag attggtgaac gtatggtgtc tgcggctgga gggatgccag tatcacgcct
      721 gaaatctgtg gctcactttg aagatgaaga ctggacgcgt ttctcacaag gggtggggcg
      781 gatgaccggg cgcaatatct ggatggtgga ccaggcgaac ctggccattg atgagatatg
      841 cgcaacaacg aaacaccacc tgattaaata tccggaaacg gcgctggtgg tggttgatta
      901 tctcgggctg ataaaaaccc gaaccacggg gcgtcatgac cttgccgtgg gtgaaatctc
      961 aaaggggctt aaaggcctgg caaaatccgg tggttttccg ttgattgcgc tgagccagct
     1021 ctcccgcggt gtggagtcca gacccaataa acgtcccatg aactcagacc tgaaaaattc
     1081 cggagaaata gaggcggatg cagacatcat tctgatgctt tacagggatg aagtgtacaa
     1141 cccggatacg caggccaggg gcatcgcaga aatcaatatc acgaaacaac gtaacggttc
     1201 tctgggaacg atttaccggc gtttttataa cggacatttt ctgcctgtgg accaggaaag
     1261 tgcacgggtg ctttccactc ccatgaagcc cggcaatccg cgcagataca gtaataaacg
     1321 aactgacagc agtaagatgg agcgtttctt ttgaacaacc agacaatgac ttttacccct
     1381 gaacaattgc gtaaacacgc gcaggaaatg ctgcgtcatg ctgaacaact cgaaaaaacg
     1441 ggcataacaa aagatgctat ccgtaaagat atggtacccg cgcttcggga actgatgcag
     1501 gcgaagcatc gcgcacagaa agcagtagat gagctggtgg actgtgtggc agagctggaa
     1561 accagagtcg gaaagtttga aaaactggtg caggaggcgc tgcgctgatg cgccatgagt
     1621 ttgttttgcc ctggcttgtg cgcgatcatt atgtccctgg tggaaagccc gggataaaaa
     1681 tcaccgaacc ggagagtatt tgatgagcgt caaaattcaa acgataccgg aactgcttat
     1741 ccagacacga ggtaatatga ccgaagtttc acgaatgctg aactgtaatc gcgccacggt
     1801 aagaaaatat gctgaagata aagagggcaa agggcacgcc attgttgatg gtgttctgat
     1861 tgttcatcgt ggatgggaca ggggtaaaga cagtgatgcg tgatattcag caggttcttg
     1921 agcgctgggg cgcgtgggtg gctaataatc atgaggatgt gacctggtcg tccattgctg
     1981 ccggttttaa gggattaatc ccttcaaaag taaaatcccg cccgcaatgc tgtgacgatg
     2041 acgcgatggt tatatgtggg tgtatggccc gccttaaccg gaacaacaac gatctacatg
     2101 acttgctggt tgactactac gtgtttggga tgacattcat gacgctggcg cggaagcatg
     2161 gatgctcaga tggttatata ggaaagaagc tacaaaaagc cgagggggtg gtggaaggaa
     2221 tgttgatgat gttgggtgtc cggttagaaa tggacaaata tattgaacat ccctgatggt
     2281 aaggggcgac tttctagaaa atattttacg tacgtaaaaa acgggatatt ctgttaagag
     2341 tggttgcttc gccacacagc ttaaacccgc ctccgggcgg gttttttatg cctgaaaaac
     2401 ggtacaggac gttaaacacg ctggtggtca gatgagtttg cagatgtgat gacatatggt
     2461 tattattctg cctccggccc tttagctcag ttggtcagag cgagcgactc ataatcgcca
     2521 ggtcgctggt tcaagtccag caagggccac caaccaccac tagctcatcc ggatagagca
     2581 tcaaccttct aagttgacgg tgcgaggttc gagtcctcgg tggtgggcca gcgccgactt
     2641 agctcagcag gcagagcaac tgacttgtaa tcagtaggtc accagttcga ttccggtagt
     2701 cggcaccata tgcgggcatc gtataatggc tattacctca gccttcca
//
LOCUS       DAJVPT010000224         2716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVPT010000224 DAJVPT010000000
VERSION     DAJVPT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(18..422)
                     /locus_tag="OQM47_004005"
     CDS             complement(18..422)
                     /locus_tag="OQM47_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481933.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            complement(974..1690)
                     /locus_tag="OQM47_004006"
     CDS             complement(974..1690)
                     /locus_tag="OQM47_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481934.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            complement(2275..2716)
                     /locus_tag="OQM47_004007"
                     /pseudo
     CDS             complement(2275..2716)
                     /locus_tag="OQM47_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          826 a          597 c          522 g          771 t
ORIGIN      
        1 actattgctg ggtaagatca catctcatcg cttacagcca ctacaggacg tttccgtatc
       61 ctgtgaatta tcatcccacg tggactgcat atatttttaa tacacacaac ccctcttcca
      121 gccacatgca gtgtcgaggt ggtcccgcag attccgtata cccgacaccc tgcacgggtt
      181 ccgagtatct gtactcccgg tggtttatca gagtaatagt agtcactgtt acattcgacc
      241 cagctctttc ccccgggggg atgcgggacc actcggaaat acaccccata tggtccgtgc
      301 acattaatct ccgcatagca atgttctgtt gaattacgtg gcatagccag taaagtctct
      361 tttgcagcag cgggtaccgc ataccacata cacattagtg caactatcag cgcagtattc
      421 attttcattg tcactttccg ttactggtgc tgtccgtcac agcacctcat gtcctgcgga
      481 cagggcaagc tgaccggcca taacaacttt cagttccatc agggaagtgg aagctagacg
      541 ccggacaatc aaatcccgcc ggcggtacac cttcttcgca gcagagttgt tcagtcaaca
      601 tgaaagtgac agacagtgtc accacgatgc agcatcagta tgacggtgaa tcgccggtcg
      661 tagtttttgt tctttgtttt atagggagct ttctgcatca ggcatcgttc atcacggggt
      721 attggtgcaa tgctcggcat cactcagtcc ggctggtgat ttgtgatgtt tggcaattta
      781 tctgattaca taatccggac tgagtccccc ctgagtgttt tactgttccg agcacttatt
      841 tagtaacttt cttgcatagt tgtttctggc tttaattgtg ctgccctaag gtttgtttct
      901 cagtatgaca gctcaataca tatacttcga acgcattata tattcattta attcactttg
      961 tgggagagga ttattacttt ctgacataaa catcattgat ttcataaaat cccgactgtt
     1021 ctgtattaaa tattaagaat ctgttactgt catctttaaa tgaactagat aaaacgagcc
     1081 gtgaatcata acgagcaaga acataatacc aactattatt ataacaaagc atctgatatt
     1141 ctttttttaa ttgaggttta taccagccaa caagaaagga aaaaaaccaa aaataaacca
     1201 aaaaaccagc cataattaac ttaatgcggt gttctctaac aaaagggata tctgaaaaac
     1261 atgatacaga aaagatccgt ccatatatac gaattattat tgaaataacc aatgaactaa
     1321 taatgaatga aagtgataat ggaagtatga aatattgttt gagaattaaa agttcgaata
     1381 ttacagggaa aaacagtagg gtaatagcaa aaaatagtct aaaaaaactc aagtcttgta
     1441 ttttacttct tttctttata agaagaaaaa ataaaatacc aatcccccaa ccggtaaaaa
     1501 gtatacccat tactatgata gcatagagta aacttctggc aacatcatcc aaacctgcac
     1561 caacaaccca ccatggaaat ccatagtaaa aagaggttcc ccagccataa gaataagcac
     1621 tgacccaccc tacacatccc atataggcga tgaataatga agaatttcta agcaatgaat
     1681 tgtcattcat aagaaactaa ctgataatgt aaaaaatatt cgagataaaa gttgatcttt
     1741 taattttcat gctaggaact taacaataat atcattgtta atgcatattt atcatatcaa
     1801 aatactctgc agaatgctac tatcaaaacc agacaagtga acaaaacttg gtcaacattt
     1861 agtaacgcta gttggtaatg tcttcaatcg atagaagttt ctggtaatct actggtattc
     1921 aaacagttag ggctcataca gcttgctgga ccttccccat aaccgcccga acgtgaaggt
     1981 ctgatgtgtt gcaagagagc cggatgcggg aaatctgcaa gtccggttcg atgagaggga
     2041 cgttgttttt acttgttgtt gatgaagact ttcattattg aacagaatga taatattgtc
     2101 taaacaaaac attaaatctc ataattcttt gtagaaaacg cttttcttga aattaaaaat
     2161 aagtagaatc agcagtctca ttataataac agacaatgtc atgctctcgc ccgttccggt
     2221 aataaacgga gcgggctatc tttttggaat atttatggcc cacaatccag tatttcataa
     2281 atacgattca ttccattctt cgttttttct gagcattgcg ttcataatgg tcagcaactt
     2341 acgcatacag gcaaccagtg ccactttttt ggcgtttccg gctgcaagta ggcgcatgta
     2401 aaacgccttt atcaccagat taaaccgggg ggggcctcaa gcgccgccat gtacagagct
     2461 gttcttcccc agcacaatca ccgaaaatgg ttcgtcgacc gcgcagagtt cccgagtccc
     2521 ggtttacctg agcaacaccg acaagtgcgc tgatttcacg tctcgagagg ctgctcgatt
     2581 caggcatttc cgccagcaat gctgcggcag tcatggctct aacacctcag acgttcagta
     2641 agctctttaa agtaactctg aatgtgcctg ttcatatctg gggcttcccc gatcatggtg
     2701 ggaaggctta gaacgc
//
LOCUS       DAJVPT010000225         2691 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVPT010000225 DAJVPT010000000
VERSION     DAJVPT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..2046
                     /locus_tag="OQM47_004008"
     CDS             <1..2046
                     /locus_tag="OQM47_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1481935.1"
                     /translation="LVPDGLPVSGHATATLNLPQEPSLVDAELDWQENSGQLIVLARD
                     NGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLSGRLGVKVDNWQAGLENALVSGRLN
                     VLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTGEAKQADLILYARLPAQLSGSLSDP
                     TLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKVTQRGVDGRLQAILQAHENELGDFV
                     LHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATWDVAGKGEWHDSTITLTDLSTGFDQ
                     LQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGALSLDAGQTLFTGGSVLPPSTLKFSV
                     DGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLRGNAWWPKQSLTVFQPLVPPDWKMN
                     LRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAWMPDNQVNGVDFVLPFRFADGAWHL
                     GTRGPVTLRIAEVINLVTAKNITADLQGRYPWTEEEPLLLTDVSVDVLGGNVLMKQLR
                     MPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAFSGALPLWLNNEKWIVKDGWLANSG
                     LMTLRLDKDTADAVVKDNMTAGSAINWLRYMEISRSSTKINLDNLGLLTMQANITGTS
                     RVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQAWLEQNARLPGNDCPQGKECEEKQ"
     gene            2043..2228
                     /locus_tag="OQM47_004009"
     CDS             2043..2228
                     /locus_tag="OQM47_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1481936.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2233..2562
                     /locus_tag="OQM47_004010"
     CDS             2233..2562
                     /locus_tag="OQM47_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1481937.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
BASE COUNT          695 a          589 c          758 g          649 t
ORIGIN      
        1 ctcgtgccgg atggacttcc tgtaagtggg catgctactg cgacgttaaa cttgccgcag
       61 gaaccgtcac tggtggatgc cgagctggac tggcaggaaa atagcgggca attgatcgtg
      121 ctggcacggg ataacggcga tccgttgctc gatttgccgt ggcaaattac tcgtcaacaa
      181 ttgaccgtaa gcgatggtcg ctggagctgg ctgtatgctg gttttccttt gagtggccga
      241 ctgggtgtca aagtcgacaa ctggcaggca gggcttgaga acgctctggt cagcggacga
      301 ctgaatgtgc tgacccaggg gcaagcgggt aagggcaacg cggtgcttaa ttttggccca
      361 ggaaaattaa gcatggataa cagtcaactg cctctgcagc tgaccggtga agcgaaacag
      421 gcggacctca ttttatatgc ccgtttacct gcgcagctaa gtggaagtct gtctgaccca
      481 acgctgacct ttgagccagg cgcgttactt cgttcaaagg gaagagtcat cgattcgctg
      541 gacatcgatg aaatccgctg gcctttagcg ggtgtaaaag tcacccaacg tggtgttgac
      601 ggacgtttgc aggccatttt gcaggcgcat gaaaatgaac tgggcgattt cgtgctgcat
      661 atggatgggc tggcgaatga ttttctccct gacgctggcc gctggcagtg gcgctactgg
      721 ggaaaaggga gttttacacc gatgaatgcc acctgggatg tcgcaggaaa aggtgagtgg
      781 catgacagca cgattacgct gaccgatctc tccaccggtt tcgaccagtt acaatacggt
      841 acgatgacgg tagaaaagcc gcgattaatt ctcgacaagc ccgtcgtctg ggtacgtgac
      901 gcacagcatc cctccttcag cggcgcgctg tcactggacg ccgggcaaac gctgttcact
      961 ggcggcagtg tgttaccgcc atcaacctta aaatttagcg tcgatgggcg caatccgacc
     1021 tatttcctct ttaaaggcga tttacatgct ggtgagattg gcccggttcg ggtaaatggt
     1081 cgctgggacg gtattcgtct gcgcggtaac gcctggtggc caaaacaatc actgactgta
     1141 ttccagccgc tggtgccacc cgactggaag atgaacttac gcgatggtga actgtatgct
     1201 caggttgcat tttctgctgc gcctgaacaa ggattccgcg cgggagggca cggcgtgttg
     1261 aaaggcggta gtgcctggat gccagataat caggttaacg gtgtcgattt tgtcctgcct
     1321 ttccgttttg ccgatggagc ctggcatctg gggactcgcg gccccgttac gttgcgaatt
     1381 gccgaagtga ttaatctggt gacagcgaaa aatattacgg ctgatttgca agggcgttat
     1441 ccgtggactg aagaagaacc cttgctgttg actgatgtta gcgtcgatgt gttaggcggt
     1501 aacgtgctga tgaaacaatt acgtatgccg caacatgacc cggcgctgtt gcggctgaat
     1561 aatctctcat ccagcgaact ggttagcgcc gtcaatccga aacaattcgc catgtccggg
     1621 gcatttagtg gtgcattgcc gttatggcta aacaacgaaa aatggatagt gaaagatggt
     1681 tggctggcaa atagcgggct aatgaccttg cggctggata aagataccgc agatgcggtg
     1741 gtgaaagaca atatgactgc gggttcagca attaactggt tgcgctatat ggaaattagc
     1801 cggtcatcga caaaaattaa cttagataat ctcggtttat taaccatgca ggccaacatt
     1861 acaggtacca gtcgcgttga tggtaaaagc ggtacggtaa accttaatta ccatcatgaa
     1921 gagaatattt ttacgctgtg gcgcagttta cgctttggcg ataatctcca ggcatggttg
     1981 gagcagaacg cacgtctgcc gggaaatgac tgtccgcaag gaaaagagtg tgaggaaaaa
     2041 caatgaaaat tttactggct gcgttgacgt catcttttat gctggttggc tgtacgcctc
     2101 gcattgaagt cgctgcacct aaggaaccga tcactatcaa tatgaacgtt aaaattgagc
     2161 atgagatcat catcaaggca gacaaagatg tcgaagagct gcttgaaact cgtagcgatc
     2221 ttttctgagg cgatgatgaa gaaaacatta cttctttgtg cgtttcttgt tgggctggta
     2281 agcagcaatg taatggcatt gactctggat gaagccagaa ctcaggggcg ggtaggtgaa
     2341 acattttatg gttatctggt tgcgctgaaa acggatgctg aaacagagaa attagtaacc
     2401 gaaattaatg ccgaacgtaa agcgagttac caacaacggg caaagcaaaa taatgtgtct
     2461 gtagatgata tcgcgaaact cgccgggcaa aagctcgtag ctcgggccaa accgggggaa
     2521 tatgtacaag ggataaacgg taaatgggtg cgaaagtttt aatagcaaaa ccgcaatatc
     2581 gggataacgc tacaggagaa agcgattgat ctattttcct gaaacaaggt gaatattcaa
     2641 aaactcctgt caaattgcct ttcgccctga aaaatgcata gtcaaatatc t
//
LOCUS       DAJVPT010000226         2644 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVPT010000226 DAJVPT010000000
VERSION     DAJVPT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            10..687
                     /locus_tag="OQM47_004011"
     CDS             10..687
                     /locus_tag="OQM47_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1481938.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..1034
                     /gene="tnpB"
                     /locus_tag="OQM47_004012"
     CDS             687..1034
                     /gene="tnpB"
                     /locus_tag="OQM47_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1481939.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1054..2625
                     /locus_tag="OQM47_004013"
     CDS             1054..2625
                     /locus_tag="OQM47_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1481940.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          638 a          725 c          736 g          545 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa ggatgacccg ttctccggac acctgttcat cttccgcgga cgccggggtg
      841 accagataaa agtgttgtgg gctgacagtg acggactgtg cctcttcacc aaacgcctgg
      901 agcggggccg cttcgtctgg ccagtcaccc gtgacggcaa ggtgcacctt actccggctc
      961 agttatccat gcttcttgaa ggtatcaact ggaagcaccc gaaacgaacg gaacgcgctg
     1021 gaatccgcat ataacccgtt gtaaagtgag gatatggaca cctcacttgc tcatgagaat
     1081 gcccgcctgc gggcactgtt gcagacgcaa caggacacca tccgccagat ggccgaatat
     1141 aaccgcctgc tctcactgcg ggtggcggct tatgcttccg aaatcaaccg gctgaaggcg
     1201 ctggttgcaa aactgcagcg catgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa
     1261 accgaacgac agatacagga agcacaggag cgaatcagcg cacttcagga agaaatggcg
     1321 gaaacgctgg gtgagcaata tgacccggta ctgccatccg ccctgcgcca gtcttcagcc
     1381 cgtaaactgt taccggcctc acttccccgt gaaacccggg tcatccggcc ggaagaggaa
     1441 tgctgtcctg cctgtggtgg tgaactcagt tctctgggat gtgatgtgtc agagcaactg
     1501 gagcttatca gcagcgcctt taaggttatc gaaacacaac gtccgaaact ggcctgttgc
     1561 cggtgcgacc atatcgtgca ggcaccagta ccttcaaaac ccattgcacg cagttatgcc
     1621 ggagcggggc ttcttgccca tgttgtcacc gggaaatatg cagaccatct gccgttatac
     1681 cgccagtcag aaatataccg tcgtcagggc gtggagctga gccgtgccac actggggcgc
     1741 tggacaggtg ctgttgctga actgctggag ccactgtatg acgtcctgcg ccagtatgtg
     1801 ctgatgcccg gtaaagtcca tgccgatgat atccccgtcc cggtccagga gccgggcagc
     1861 ggtaaaaccc ggacagcccg gctgtgggtc tacgtccgtg atgaccgtaa cgccggttca
     1921 cagatgcctg cggcggtctg gttcgcgtac agcccggacc ggaaaggcat ccatccacaa
     1981 aatcacctgg ccggttacag cggagtgctt caggccgatg cttacggtgg ctaccgggcg
     2041 ttatacgaat ccggcagaat aacggaagcc gcgtgtatgg cccatgcccg gagaaaaatc
     2101 cacgatgtgc atgcaagagc gccaaccgat atcaccacgg aagccctgca gcgtatcggt
     2161 gaactgtatg ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcagca
     2221 agaaaagcca gagctgcgcc actgatgcag tcactgtatg actggataca gactcagatg
     2281 aaaacactgt cgcgtcactc ggatacggca aaagcgttcg catacctgct gaaacagtgg
     2341 gaggccctga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgcagag
     2401 aacgccttac ggggagtggc cgtaggccgg aaaaactggc tgttcgcggg ttccgacagc
     2461 ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     2521 gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc ggcaaaccgg
     2581 gtacgcgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     2641 tgac
//
LOCUS       DAJVPT010000227         2586 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVPT010000227 DAJVPT010000000
VERSION     DAJVPT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            35..1035
                     /gene="gap"
                     /locus_tag="OQM47_004014"
                     /pseudo
     CDS             35..1035
                     /gene="gap"
                     /locus_tag="OQM47_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(1077..2516)
                     /gene="aldA"
                     /locus_tag="OQM47_004015"
     CDS             complement(1077..2516)
                     /gene="aldA"
                     /locus_tag="OQM47_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1481941.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
BASE COUNT          654 a          676 c          599 g          657 t
ORIGIN      
        1 attttcatcc gaacgttcct gacaggagaa aaccatgagt aaagttggta ttaacggttt
       61 tggtcgtatc ggtcgactgg tgttgcgtcg tttacttgaa gtcaaaagca acatagacgt
      121 tgtcgctatt aatgatctta cttccccaaa aattctcgcc tatctgctga aacatgattc
      181 aaactatggt ccgttcccct ggagcgttga ttttacggaa gattcactta tcgttgatgg
      241 gaaaagtatc gcggtttacg ccgaaaaaga ggctaaaaat attccgtgga aagcgaaagg
      301 ggcagaaatc attgtcgaat gtactggctt ttatacctcc gccgataaat cgcaggcgca
      361 tcttgatgct ggtgcgaaga aggtgttgat ttccgctcct gccggtgaaa tgaaaaccat
      421 cgtttataac gttaatgacg acactctgga tggcaacgac accattgttt ccgtggcgtc
      481 atgcaccact aactgccttg cgccgatggc caaagccttg catgacagtt tcgggataga
      541 agtcggcacg atgacgacca ttcatgccta tactggcacc cagtcactgg tggatggccc
      601 gcgtggtaaa gatttacgtg cttcacgcgc agcggcagaa aatatcattc cccacactac
      661 gggggcggca aaagccattg gtctggtgat cccggaactg agcggcaaac tgaaaggtca
      721 tgcgcaacgc gtgccggtga aaacaggttc ggtcactgag ctggtgtcca ttctcggaaa
      781 aaagtgactg ccgaagaggt gaataacgca cttaaacaag caaccaccaa taacgagtca
      841 tttggttata ccgatgaaga aatagtctct tccgatatca ttggcagcca tttcggttca
      901 gtgtttgatg ccacgcaaac ggaaattacc gccgtgggcg atttacaact ggtgaaaacg
      961 gtcgcctggt acgataacga atatggcttc gtcacgcagc ttattcgcac cctcgaaaaa
     1021 ttcgctaaac tctgacgcgc acaggcggag gaaaaaacct ccgcctcttt cactcattaa
     1081 gactgtaaat aaaccacctg ggtctgcaga tattcatgca ggccatgttt accatctgcg
     1141 ccgccaatac cggatttacg ccatccggcg tggaaacctt gcatagcttc gaagttttca
     1201 cggttgatgt aagtttcacc aaacttcagc cctttaatgg ctttcatcgc gacgttcaga
     1261 ttttgggtat agattgatga ggtcaggccg taatcactgt cattagccat tgagatagca
     1321 tcttccagcg tgtcaaatgc gacaactggc agcaccgggc caaaggtttc ctcatgcata
     1381 atcgacattt cctggcgaac atccagcagc aatgtcggcg gataataata tcctttcccc
     1441 tctaccgctt tgccacccaa cgccactctc gccccttctt ctactgcgcg cgccactttt
     1501 tgctcaaccc tttccagcgc cgcggcgtta atcaacggcc ccatcgcaat gtcgttgcgt
     1561 tcagcggggt taccaaattg aaccgcctgc aacgcttcac ccagcagatt gacgaactga
     1621 tcataaatgc ctttctgtac ataaacacgt tctgcacagt tacacacttg cccactatta
     1681 atgacgcgtg aatcaacgat ggctttgact gccagttcaa gatcggcatc gtccattacg
     1741 atagccggtg ctttaccacc caattccaga cacactttgg tgatgttttt cgccgcagtc
     1801 gccatgatct tctcacctgc agagacgctg cctgtcatac tgaccattgc gacctttggg
     1861 ttacccgcca gttcttgccc aacggtttca ccacgcccca gtacaaggtt aaacacgccg
     1921 cgcggaaggc ctatttcatc gacgattttg gcgaatgcaa tcgcattgtt tggcgtaaat
     1981 tcactgggtt taatgacgat ggtattaccg gtcaaaagag cgggagccat tttgcgggca
     2041 atgaggaaga acggaaagtt ccacggcaga atgccggtag tcacaccaag cgcacgttta
     2101 aacagaagaa tattttctcc cggacgatcg ctttgaataa tctcgccctc gtaacgccgt
     2161 gcccactccg ccatgtaatc gatatagtcg gcagtaaaag ccacttcgac ttcagctagc
     2221 tgctggatct tgcccccttc ttcaacaatc agcgcactga tttcactggc gcgttcgcgg
     2281 atcccggcgg agattttgca caaccaactg gcgcgttcaa tagcaggcaa cgcttcccat
     2341 tctggttgtg cacgttctgc tgcatcgatt gccttacggg catcctcggc ctgaccatcg
     2401 ggtatgcggg aaatgacagc ctctgtagca gggtttacca catcaatcca tgcgtctcca
     2461 cgccaggtaa caaactgtcc atcgatatac ataggatgtt gaacgggtac tgacatgggc
     2521 gactcttgtg atttatatgt ttgttttcgg tgaatatatt gttaattaac atcaatcatc
     2581 agagag
//
LOCUS       DAJVPT010000228         2565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVPT010000228 DAJVPT010000000
VERSION     DAJVPT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            50..>2565
                     /locus_tag="OQM47_004016"
     CDS             50..>2565
                     /locus_tag="OQM47_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1481942.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVT"
BASE COUNT          689 a          558 c          647 g          671 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatg
//
LOCUS       DAJVPT010000229         2561 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVPT010000229 DAJVPT010000000
VERSION     DAJVPT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..261)
                     /locus_tag="OQM47_004017"
     CDS             complement(<1..261)
                     /locus_tag="OQM47_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1481943.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFA"
     gene            454..957
                     /locus_tag="OQM47_004018"
     CDS             454..957
                     /locus_tag="OQM47_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1481944.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OQM47_004019"
     CDS             complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OQM47_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1481945.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1581..>2561
                     /gene="argF"
                     /locus_tag="OQM47_004020"
     CDS             1581..>2561
                     /gene="argF"
                     /locus_tag="OQM47_004020"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1481946.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAV"
BASE COUNT          718 a          599 c          591 g          653 t
ORIGIN      
        1 tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa acattcccac cggtaattcc
       61 ataagaaaat cgttgtccgt taacttccat attagcataa agataacgct tacatttcat
      121 taatgcccat ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca
      181 aataagaaaa tatctctcac ctgtaccatg aaaaacaaaa gaatgggacg gaacatccat
      241 ttcattaata acttgagcca taaaaatccc ctgaattaaa aaatccattg atataattta
      301 tagatacaga cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc
      361 aaaaacttta ttgctttata agacgcttgc gcacgtaaga agagagtggt attaaacaaa
      421 tgtataatta ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct
      481 gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat ccgtcaggtt
      541 tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc gaatctcgac
      601 gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga atacaatggc
      661 gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga catttgcgaa
      721 ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa aaaactggcc
      781 ttaatggcga tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc
      841 gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca tatcgactat
      901 gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga actctaaaaa
      961 aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc gaaccccgtc
     1021 atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat cttcaaagta
     1081 agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca gttgttcaac
     1141 ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc agcaaatcac
     1201 gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac cgagtttaaa
     1261 cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat ggtgttcgat
     1321 ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt
     1381 ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct cttaatgtcc
     1441 tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg acaaagattt
     1501 atgctttaga cttgcaaatg aataataata cacataaatt gagttttaat tcattgaggc
     1561 gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg aaattactcg
     1621 atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg aaagctgata
     1681 agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa
     1741 aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag ggggctcgcg
     1801 ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt aaagacactg
     1861 cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt caggagattg
     1921 tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc aatgagtttc
     1981 accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc ggtaaagcat
     2041 tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc aattcgatgc
     2101 tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag gcatgctggc
     2161 cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc gggaatatta
     2221 cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat accgatgtat
     2281 gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta ctgcgtgatt
     2341 atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa ttcctccatt
     2401 gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg gaagagtttg
     2461 gcctacatgg cggaatggaa gtgactgatg aggtcttcga atctgccgcc agcattgtat
     2521 ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt g
//
LOCUS       DAJVPT010000230         2475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVPT010000230 DAJVPT010000000
VERSION     DAJVPT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..419
                     /locus_tag="OQM47_004021"
     CDS             <1..419
                     /locus_tag="OQM47_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1481947.1"
                     /translation="ALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRY
                     DANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAH
                     LAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            768..1940
                     /locus_tag="OQM47_004022"
     CDS             768..1940
                     /locus_tag="OQM47_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1481948.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKESFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(1994..>2475)
                     /locus_tag="OQM47_004023"
     CDS             complement(1994..>2475)
                     /locus_tag="OQM47_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1481949.1"
                     /translation="IQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVV
                     DIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPL
                     AAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          775 a          496 c          474 g          730 t
ORIGIN      
        1 ttgcgctgaa tgatactgca tgtcgcgtat tgaaaaaaca aatcgggaat catcaccgtt
       61 gggtatttgt gtacaaggaa agctgtacca aaccagacgg aacgaaagcg ccaacggtca
      121 ggaagatgcg gtatgacgca aacacagcct ggaaagcggc gctgagacga gcaggtattg
      181 atgatttcag atttcacgac ttgagacaca cctgggcaag ttggctggtt caagccggag
      241 tcccgttgtc agtgttacag gaaatgggag gctgggagtc tatcgaaatg gttcgtcgat
      301 atgctcacct tgcacctaat caccttaccg aacacgcacg gcaaatagac tcgatcctga
      361 acccatcggt cccaaattcg tcccagtcaa aaaataagga aggtactaat gatgtgtaac
      421 ttattgattt aaatggtgcc gataatagga gtcgaaccta cgaccttcgc attacgaatc
      481 tgtagcacca atcataacta tctgttttag caagcattaa ccgcattcac taagctatag
      541 ttgatgacac aaacagaaag ttgatgcatg atgttgccat atatatgtca caaatacggc
      601 acaacgatct tcaaacatat agccacacat tcacagaaga gcacaaagcc ttgcaatcca
      661 gtgcaaagct tggtgtgtct ccgttttgtt tcatcagtta cacataacta tatgatcatt
      721 aactaaaagt cagtatactt ggagaaattt taattgcgta atagagcatg catatgattg
      781 aaattaacag tttattatta ataacatccg tgatattgat gtcgttatta gctgtggggt
      841 tatttgataa aatttcccca ataaatcttg ttgagcacgg caggaataat caaatagatg
      901 gaatgcgggg gttcttagca attttcgtgc ttattcatca cgcagcaatt tggaatggat
      961 acttgtcatc tggagtatgg gaagcacctt catcaaatct gttagcaaat ttaggccaag
     1021 ttggtgtgtc attctttttt atgattactg gttatctgtt cttttcaaag attatctctg
     1081 gagatcagga ctggacaagg ctttatgtat caagattact acgattaacc ccaatgttca
     1141 tagttagcct atgtctcatt tttatcattg taggttttaa gtctggatgg agaatgcagg
     1201 tatccacaga agagcttttt gtgtcaataa tgaagtggtt gccattcact gcgctaggta
     1261 tgccgaacat taatgacgta aaagagtcat ttactatcaa tgccgctgta acatggacac
     1321 ttgtatatga atggttcttt tatttttctc ttccggtaat ttccgcgctc ataaaaagaa
     1381 aagtcagtat ttacatggtt atgattagcg caatatcgct atttgttttc attttatttt
     1441 tcagtaaaat acacatagca tcatttcttt ttgggctcct ggcatttttg ctaaataaat
     1501 caaagatagt caatggaatt gccaaagcaa aagtaacacc aattataata actgcgataa
     1561 tggtttttga aatgacgtat ttcaaaacaa cttacgcacc gctaccactg attctttgtg
     1621 gaataacatt tatcattatc gcatcaggtt gtgatttgta tggaatatta agattaaata
     1681 taaccagaaa gctaggagaa actacctaca gcgtttacct tctgcatggg atattccttt
     1741 attgcctaat gacgtggatt attcctaata attacactga aaatacattt atcatactgg
     1801 taagcacaac tgcatttctt attacgttca catcatgcct aacttttaaa ttaatagaga
     1861 cgccattcat caaattaaca aaacaaacca caactttagt aaaagaatta atacccacat
     1921 taacaaacaa caatcaataa acaataaaca taagccgccc tacatatatg tccaaatggg
     1981 gcggcgaatg tatttatatt agtctggtaa tgctaaatgc aaacgaactg gagattcctg
     2041 ccccgctaag cgtattgcgc agggtcaccc taaatgtatc tacgccaata taagtaacat
     2101 cacaactaac tgaatcaggc gcagatatag gtgttatctg cacaatataa ccgctagcaa
     2161 ctgctgcgag cggatgagaa aagctaacat catacacacc tgtagaaacc acgttaaccg
     2221 atgaaacacc agtagcaacg tcgatactac cattggcgtt aatttttccc gagcgtgtga
     2281 ctgacaatgc agaagccgat gttaccttac tggttgtttc tgtaaaaaca cacccaatgt
     2341 caactacctc gcaattagct cctgttatcc agtatggtcg ttctgcactt ggctgataat
     2401 cccctgcgct ctggaacgtt gtgccgttga gtatgactgt aacttttccc ccacctgagc
     2461 ttgttacttg aatat
//
LOCUS       DAJVPT010000231         2473 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVPT010000231 DAJVPT010000000
VERSION     DAJVPT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(64..822)
                     /locus_tag="OQM47_004024"
     CDS             complement(64..822)
                     /locus_tag="OQM47_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1481950.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQM47_004025"
     CDS             complement(830..1933)
                     /locus_tag="OQM47_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1481951.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2473)
                     /locus_tag="OQM47_004026"
     CDS             complement(1943..>2473)
                     /locus_tag="OQM47_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1481952.1"
                     /translation="PIAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPEL
                     AALTLALSVYTSAFIAEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVII
                     PPLTSQYLNIVKNSSLAAVIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTI
                     SLLMNIYNRRIAIVER"
BASE COUNT          679 a          662 c          577 g          555 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cgg
//
LOCUS       DAJVPT010000232         2379 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVPT010000232 DAJVPT010000000
VERSION     DAJVPT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..349
                     /locus_tag="OQM47_004027"
     CDS             <1..349
                     /locus_tag="OQM47_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1481953.1"
                     /translation="THAERISAMLTSPAMTETHDFSYRSVILTLSERKVLRLLGKGWG
                     INQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAE
                     WNTAELRREMSHS"
     gene            307..984
                     /gene="bglJ"
                     /locus_tag="OQM47_004028"
     CDS             307..984
                     /gene="bglJ"
                     /locus_tag="OQM47_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1481954.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1022..1810)
                     /gene="fhuF"
                     /locus_tag="OQM47_004029"
     CDS             complement(1022..1810)
                     /gene="fhuF"
                     /locus_tag="OQM47_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1481955.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1951..2187
                     /locus_tag="OQM47_004030"
     CDS             1951..2187
                     /locus_tag="OQM47_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1481956.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2226..2312)
                     /locus_tag="OQM47_004031"
     tRNA            complement(2226..2312)
                     /locus_tag="OQM47_004031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Leu,seq:cag)
BASE COUNT          609 a          533 c          619 g          618 t
ORIGIN      
        1 tacgcacgca gagcgtatca gcgccatgct gacctctccg gcaatgactg aaactcatga
       61 ttttagctat cgctccgtca ttctcactct ttcagagcgc aaggtactgc ggctattagg
      121 taaaggatgg ggcatcaacc agatagcttc attgcttaag aaaagtaata aaactatcag
      181 cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc
      241 atggataaat agcgcgcagg gtgcaagaga acttaacttg ccttctgttt atggagatgc
      301 cgcagaatgg aacacagccg aattaagaag agaaatgtcg cactcataga aaaatgcgtc
      361 atgagtagta tcggtattga gagtttattc agaaagtttg cgggcaaccc ttataagctc
      421 catacctata ccagtcagga gtcatttcag gatgccatgt cgcggatctc gtttgcggcg
      481 gtcatttttt ctttttctgc catgagaagt gagcgcagag agggattatc ttgcctgact
      541 gagctggcga ttaagtttcc gcgtacccgg cgtttagtta ttgtggatga tgatattgaa
      601 gctcggctga ttggttcatt gtcgccatca ccgctggacg gtgtattaag taaagcgtca
      661 acgctggaga tttttcatca ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc
      721 gaccgactga acaatcagtg gtacattaac caaagccgga cgttaagccc gacggagaga
      781 gaaatattgc gctttatgtc gcgtggctac tcaatgacac aaattgccga gcagcttaaa
      841 cgtaatatca aaacgatccg agcgcataaa tttaatgtga tgtcgaaact gggcgtcagt
      901 tctgatgcag ggttgttgga ggccgaagat attctgttat gtatgcggca ttgcgaagca
      961 agtaatgtgt tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct
     1021 ttcatttcag cgtacaatag ccacattgct gcacatccgg taagcgataa cgctggcagc
     1081 aagtgcggcg caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt
     1141 caccgttcgt gagcgttttc tcaaaaaaga gggcatggcg cagcgattca acagtagcct
     1201 cgccgagcag ttgcttcatc tcagtgagat accagttgat caaataaccg gtattactcc
     1261 agataagttt gccgttgatc tctccggtcg cttctagtgc ttgcacaacc ggaaccagcg
     1321 cctggctgat taacgtttcc attcgctgct gcggcgaatg tggtgttgcg tttttatctt
     1381 cacacacatc gacccagaaa caggcgacgc gtccggtttc gtgaaactca gcatggaaat
     1441 gttccggcga cacatctaat gctttttcct gcgtcagtag cgccagcatt aatggtggca
     1501 ccatcaggcc gatataccat tgtgcccata gtgagatcag cggtttgttc tcgcggatca
     1561 tcgttggttg gttgcgatag atatgatcgg aatagaccgc cagtagagaa cttagcgcat
     1621 tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg agcgggttca tccaggcgga
     1681 taaactccag caaatgttca cgatgtttgg cgatcatcgc ccgtacggct tgtgcaagcc
     1741 cagcatcctg cggctggaga tgcgttcgcc agatgacatc ttcatagagc ggtgcggaac
     1801 gataggccat aatcgtgata gtaatctaaa tgataatgat tgctaatcct agcgataggt
     1861 ttactcgata gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg
     1921 gttttcatta tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg
     1981 ggagtgttgc tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat
     2041 cagtggcgga ttgtcattct gatgggatta gtattgacgc cggtgatgct gtatcacaaa
     2101 cagttgcggc attacatttt gctaccatcg tgcctggcgc ttattgctgg catcatgctg
     2161 atgataatga atttgaatca gggatgacaa aacagggaag aaacaagaaa ggaagtaaag
     2221 ataattggtg cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa
     2281 gctagcgcgt ctaccaattc cgccaccttc gcacagtcat cttacttttt ttgatatcgc
     2341 ctcgtttggt gcgagggggg ggacttgaac ccccacgtc
//
LOCUS       DAJVPT010000233         2323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVPT010000233 DAJVPT010000000
VERSION     DAJVPT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1073)
                     /locus_tag="OQM47_004032"
     CDS             complement(<1..1073)
                     /locus_tag="OQM47_004032"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1481957.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRL"
     gene            complement(1157..2320)
                     /locus_tag="OQM47_004033"
     CDS             complement(1157..2320)
                     /locus_tag="OQM47_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1481958.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
BASE COUNT          583 a          679 c          561 g          500 t
ORIGIN      
        1 agacgaaaca gcaacgccgt ttgcccttca agctgaagtg ccatgcgact taagacatga
       61 cgcataaggg gctgttggat caatggatta ccaaaaacat ggcgttgatg tgcatgataa
      121 atcgccagcg aaaatgcacg gcgcatcatg gcatggctac ccagggcgca atcaaaacgc
      181 gtcatccccc ccattttcag gatcagacga attccttccc cttcctgccc caataaccag
      241 ccaatggcat cctgaaactc cacttcgcaa ctggcgttag agcgattacc cagcttatct
      301 ttcagccgct cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg cggcacaaaa
      361 aagcaggaca gaccgcccgc ggtctgcgcc agcaccagat gcgcatcgct ttgcggcacc
      421 gagaaaaacc atttatgccc caccagccga taagagccat cttccaaacg ctctgcacgg
      481 gtggtgttgc tcatgacatc ggaaccgccc tgcttttccg tcattcccat gccaatcaac
      541 aaaccgcgtt tttgcccacc tggcaataag tgagaatcgt agcgatcgct cagcagcggc
      601 gtggtccagt cctgaaacgg cgtgggtaac atctgtaaca acaatggcgt ggcggcaaag
      661 gtcatcgtta ctggacataa cgacccagcc tcaacctgcg catgtaacat aaaacgcgcc
      721 gcgcgcgcca caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt gtgcacccga
      781 ttggtacata acgcctgcat cagcaggtgc catgcgggat ggaaacgaac atcgtccaga
      841 cgccgcccct gggcatcgta gcgcaacagt tcaggcgggt ttacattcgc cagccgcccc
      901 agttcaaggg attcagccgt tcctaactgc tgaccaatac tggctagaaa atcgctatcc
      961 cagccagcac cttcacgcgt taccgcttcg caaagcgcgc catcagacag gtataagttg
     1021 ctgttattta atggtatagg ttgattaaaa atggtgtgag tttgccagtg cactgtgtct
     1081 ccctccatca atggcagtca ccattagtat ggtcactgcc atggattcat gacagaaaga
     1141 gattgctaaa acattcttat ccagcaatgt aatgcggaac gaaacgtgag gtgtctttgg
     1201 tgatcagtgt gttatcttca cggatcccca ttccgcacgc ttcatcatcg acaatccagc
     1261 taccgatgag tgtgtagcta tcgccaaatc gcggcagagg ttgaaacgcc tggtagatca
     1321 tcggttcatc ggcgtaatca ccatcagcgt ggtcaacgac gttattctga ccgtcgaaaa
     1381 tggtgacgtt tccgccttcg cgcgagtaga tcggtttacg cacatagctt tcgccagcgg
     1441 caatctgcga tttttcaccc tcaaaccagg atgcaagaag gttaggatga ccagggaaga
     1501 agcgccaaag cagcggcatt agccctttat tactcaagat acttttccat aacggctcca
     1561 cccattgctc gcgacgcttg cgcagcagcg gaccgttatc gtcacgcatc atccactcca
     1621 gcggatacag cttaaatgca cgctggatga cattatcatc aagatcggtc agtacgccgc
     1681 cgacgcccaa accgagatct tcaatgtaga taaaccgcga ctcctgccct gcctgctggg
     1741 cgcagtcctg caagtacagc acggtactcc tgtcttcatc ggtgtcctga cagcagcaaa
     1801 aataaaacag ttcccgactg taaagctcac taaagcgcga aatcaggcgt tcctgaatgg
     1861 cgttgtactg atcggcatca cgcggaataa tgccgctgcg ccgggcatcc tccagccaca
     1921 accactggaa ataagccgac tcatacaacg aggtcggcgt atcggcgttg tactccagca
     1981 gcttcaccgg cgcattgcca caccaggcaa aatccatccg gccatacagc gaaggatcgc
     2041 gtgcgcgcca gctttcggcg atcacatccc agtacaacgg cgggatcgcc agttgcgtca
     2101 ggatctcttc gtctttaacc gcgcgatcca ccacctcaag gcacatctga tgcagttccg
     2161 cagtcggttt ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa gcccgactct
     2221 cgtcccaata gatttcattg tcgatgatat gaaagtcgaa accgttgtta gcggcgatct
     2281 ggtccagatc ccgccgcaca ggaacgttgt gtctcagcat gat
//
LOCUS       DAJVPT010000234         2281 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVPT010000234 DAJVPT010000000
VERSION     DAJVPT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..798
                     /locus_tag="OQM47_004034"
                     /pseudo
     CDS             1..798
                     /locus_tag="OQM47_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OQM47_004035"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OQM47_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OQM47_004036"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OQM47_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2124..>2281)
                     /locus_tag="OQM47_004037"
     CDS             complement(2124..>2281)
                     /locus_tag="OQM47_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1481959.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          555 c          692 g          481 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct taggggggtg
     2101 cccctattaa gttgtcaagc atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc
     2161 atcatggcga acagaacgtc gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt
     2221 cgttttccct gactcatttt gcgggtgtag taagccctgg agagcggatc cctgagcgcg
     2281 g
//
LOCUS       DAJVPT010000235         2222 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVPT010000235 DAJVPT010000000
VERSION     DAJVPT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(28..807)
                     /gene="ipaJ"
                     /locus_tag="OQM47_004038"
     CDS             complement(28..807)
                     /gene="ipaJ"
                     /locus_tag="OQM47_004038"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1481960.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1407..1544)
                     /locus_tag="OQM47_004039"
     CDS             complement(1407..1544)
                     /locus_tag="OQM47_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481961.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT          650 a          554 c          369 g          649 t
ORIGIN      
        1 gctccttcac agataaaaaa cgacaattta caaagcctca ttagttataa ctatggaaat
       61 acctatttta gtatatccta taaagttaga attacttctt cttgcaccta attccatagt
      121 tgaaaaacaa gaataatttt ctcctacagc tggatccata tagcttccat caggtctaca
      181 caacacccag tggaggccga caggaactcc aataaaggat acagcaacag cttccaaaac
      241 tctttggtta gaagataaca catctcgttg atgtactata ttacatccca tgccaattaa
      301 taagtccctt gcatcgggat aaataaaact aattacttgc gaaaaaatgt tactttcttc
      361 aacgacataa gcattcaatc caagaagcct cgtagccatg actatcttat caggcataga
      421 ataaccggca tccgcgatat tatctttgtg tattctcgga ttataattac cactagttat
      481 taagtataaa tcccgctcac aacgattatc caaatcaaga gaactcttcc ttgtcatttc
      541 ggacatagac cctttgtatt gtggtatttt atcaacccct aattcctttg cagcacagag
      601 taaactggca gctccacaag aattgtcata catctgcttc ctcgccaaaa aacaaccttg
      661 ttcgtttact ctattctcat ttaccctcac atcagtctga tgagatatca tatattctct
      721 tggtatagcc ccctgcaata aaaccccata taacataaca cctgtatgaa tacatccccg
      781 tttgcaaggc ttccgttgtt ccgacatcga cgagtaattg cagcacagta ttgcatccag
      841 caatgcgaac tactatgaga attagctgac cgggtcctta ttccccgaac gaactttaaa
      901 tccaaaacct catactcttt tccgttgtca tcacgtccgt caccataaca acctttcagc
      961 atccaccctt ttattaagag ttaatgtcca tcagcatcag caattaatac cgcctgatac
     1021 tgacatattt ttgtcgtatt tacgtccata atcggttatt gtgatacagg tcacaccagc
     1081 ttatttgcta ttgttacaat aaccaccttg gttgagagct gttttttcaa ctttgttatc
     1141 aactccccca actccttggt cagtaaggct gccgaccgag cacagtgaat agatagtatc
     1201 cgcaggtacc accatgctta cttaatactc agaaggcgaa gtgctctcca attaactctg
     1261 ccgcactatg cttataagct gcttttcaat gccgtctatc aacatgtcca gttcattcaa
     1321 tctgttgtac tggtaatcca acgcagtaat gatatatgca ggtattcttt catccactcc
     1381 agtaacgttt catgcaaagc attaatttac gcgctacgga acttcaccag ttgactgagc
     1441 aaacgatgtg ggaccagtac tgcctgcagt tcttctattg ttacagcgat ttctttacca
     1501 aacagctgaa ccaccatcaa gataaagata gcctgaacat tcatcgcacc attttgttac
     1561 caatggcaaa agatttaacg aaccgtgcct gcatcaggcg gactttttat ccctgctttg
     1621 ttagctccct aaccttgtac agagagcctc cataagctcc catatcaagc agacatggtt
     1681 cgcagttact gacatacacc agaaaactct gacagcagac tcacttatcc tccacttcac
     1741 tcatatcctt attggtgaga tgaacctgaa tgacatgttt tgcgacataa acgccaacca
     1801 gtgtatattt cacctgtaga gattctacaa tctgtagcgt tatgcatcta atgttaggct
     1861 ctatgatgtc agaaatctgc gagactctac accttctgca tttaccttta tttatcacca
     1921 tgctcctcag ttaacggaga acgtgttcat tgcattttgt cacttacatt tcgcctttgg
     1981 tatcgggggg atataggttc caacgttccg catgagaacc tgaaccggct tcacgccacc
     2041 ttgataccag atgccgcaca ggtagtcaac aagtgtcacg aacggtgcaa tagtgatcca
     2101 cacccaacgc ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta
     2161 tggtcacttt tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc
     2221 gg
//
LOCUS       DAJVPT010000236         2205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVPT010000236 DAJVPT010000000
VERSION     DAJVPT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..106
                     /locus_tag="OQM47_004040"
     CDS             <1..106
                     /locus_tag="OQM47_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCS1481962.1"
                     /translation="QSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            130..420
                     /gene="lsrG"
                     /locus_tag="OQM47_004041"
     CDS             130..420
                     /gene="lsrG"
                     /locus_tag="OQM47_004041"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCS1481963.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            460..1218
                     /gene="tam"
                     /locus_tag="OQM47_004042"
     CDS             460..1218
                     /gene="tam"
                     /locus_tag="OQM47_004042"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1481964.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1222..2136)
                     /locus_tag="OQM47_004043"
     CDS             complement(1222..2136)
                     /locus_tag="OQM47_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCS1481965.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          595 a          525 c          570 g          515 t
ORIGIN      
        1 ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggttc accataacga
       61 aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc ggatttaagg
      121 agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagacaa ggttgacgag
      181 tttatcgaag tttttcgcca gaaccacctg ggctctgtac aggaagaagg taatttgcgc
      241 ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatttacga agcctataaa
      301 gatgaagaca cagtggcatt ccataaaacc acgcctcact acaaaacctg tgtcgcgaaa
      361 ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt gatgccgtga
      421 ggctacaatt ggggtaacgc gctgacggga gtaaaaaaaa tgtctgactg gaacccctct
      481 ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg
      541 ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc
      601 ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc tccggcgatg
      661 attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac
      721 tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc
      781 gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg cgtgctggca
      841 gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcttgg
      901 gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat
      961 attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta tcaccagatg
     1021 ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag
     1081 gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat gctggaagag
     1141 cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct gtttattgtt
     1201 gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa gaatttttgc
     1261 tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc
     1321 atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca
     1381 atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag
     1441 gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat
     1501 cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc
     1561 tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgtccatt
     1621 gcggcgctga accgccaacc attctcccat tattaacaag atacggttag ctggagagtt
     1681 cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca cttccgcctg
     1741 tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa
     1801 ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta acgaccgtga
     1861 ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt acccggcatt
     1921 attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt
     1981 gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa tagaaaagag
     2041 aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc agacaaaaat
     2101 acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtggaaaagt
     2161 cgccctatta aacgtgatta cgatcacatt ctcaagacgc tactt
//
LOCUS       DAJVPT010000237         2161 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVPT010000237 DAJVPT010000000
VERSION     DAJVPT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(47..202)
                     /locus_tag="OQM47_004044"
     CDS             complement(47..202)
                     /locus_tag="OQM47_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1481966.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(405..812)
                     /locus_tag="OQM47_004045"
     CDS             complement(405..812)
                     /locus_tag="OQM47_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1481967.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            889..1116
                     /locus_tag="OQM47_004046"
     CDS             889..1116
                     /locus_tag="OQM47_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1481968.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1100..1522
                     /locus_tag="OQM47_004047"
     CDS             1100..1522
                     /locus_tag="OQM47_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481969.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1600..>2161
                     /locus_tag="OQM47_004048"
     CDS             1600..>2161
                     /locus_tag="OQM47_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481970.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQ"
BASE COUNT          569 a          444 c          565 g          583 t
ORIGIN      
        1 ttctaggagt agagcgataa cgtgttttaa ttctgtctcg ttcattttac tcacctgaat
       61 gtcttcccaa ccaacgacgt gcgccagctt cggttttaaa cgttttgctt ttggtatacg
      121 ttatggcggt gaatgtgccg tcctggttgg ggaacacgcc gtacaccgga gattcgttgt
      181 tgccaagatc gatagtattc atgttgaccc catttcccct taacgccggg gtagcggaac
      241 tgtttgctga gaacaccgtg cggtgtcttg atgaataaaa tttagaataa cctaaggagg
      301 gtgatcaaga tttttgtgta gaaaaaccta agttttttga tgtaaaaaac acaagtgttt
      361 gaaagtttgt gctttttatt acagggtgtg gagaaaaaag gggattattt gtttgcgctt
      421 cttttgcgag ctttgagtag ttcttcaaaa agtttgttga aattttcaac tcgagcacgc
      481 atctctgaca acagggcctt ttgctctgac tcaggcagtg cgtcgaacag ttgaagtaac
      541 tctttttgat cttctgtcag aatggctggc tgattatccg ggatcggttc gcctggttgt
      601 ttatcttcat ccccaaaaag aagccaagtc ggtgagcact gaagcgcttg gctcagtgcg
      661 aataatcttt tccccgccgg ctgtgtttca tctctttccc attgagaaat tgttacgtga
      721 gccactttga ccagcttacc taatgcggcc tgagacagtt ttaatttttt acgcctatgt
      781 aagaggcgag caccgaaggt ttcgtttttc atattaggga attctaattt ttcttgactt
      841 aggtttctct acggcctggt ttccttagga aaatctaagg agctcgatat gttgaaaatt
      901 gatgctatag cgttttttgg cagcaaaaca aagcttgcca atgccgcagg agttaggctg
      961 gcaagcattg ctgcatgggg ggaactggtt cctgaaggtc gcgcgatgcg cctgcaagag
     1021 gcatctggcg gggaacttca gtacgacccc aaagtttatg acgaatatcg taaggcaaaa
     1081 cgacctggga aggtgattca tgaaaatcag gcatgagcac atcgaatcag tgctgttagc
     1141 cctggcagcc gaaaaagggc aggcgtgggt cgctaacgca attactgaag aatatctgcg
     1201 gcaggggggc ggcgaattgt ctctggtacc aggcaaggac tggaacaatc agcagaatat
     1261 ctatcaccgt tggttgaaag gtgaaacgaa agcgcaaagg gaaaaaattc agaaactgat
     1321 ccctgcggtt ctggcaattc ttccgcgtga gctgcgtcac cgactctgta tcttcgatac
     1381 cctggaacgc cgtgcattac tggcggcgca ggaagcgttg agtacggcaa ttgatgcgca
     1441 tgatgatgct gtccaggccg tttaccggaa agcacatttc agcggtggtg ggtcgcccgg
     1501 cgattctgtc gtagtgcatt gattgaaatt aatcgtgccg gattgttttg ttcggtatca
     1561 gttaaatgta acgctgcgag cgttacaagg tgaaaacaaa tggcttcaaa ctggataaag
     1621 ctcgaggtta ttacgccgga taagccggaa atattcaggc ttgctgagat tctgaatatt
     1681 gatccagatg ccgcattagg gaaggttatt cgcttctggg catgggcgga tcaacaaatg
     1741 atagacggta atgcagattg taacgctcgc ggcgttacaa aaagtgcaat agatcgcatc
     1801 acttttatgt ctggttttgc tgatgcgtta attcaggttg gatggctggt cgaaaatgac
     1861 ggtgggcttt ctctacctaa ctttgaacgt cataacggaa aaagctctaa aaaacgggcg
     1921 gttacaaacg agcgagtaac aaaaatacgc gaactgaaac gaaaaggtaa cgctgccagc
     1981 gttacacaaa cggatcaaaa agcgttacca gtggaagagg aagaggaaga tctaaatact
     2041 gatctccccc taaatccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct
     2101 attgagctgc ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag
     2161 g
//
LOCUS       DAJVPT010000238         2046 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVPT010000238 DAJVPT010000000
VERSION     DAJVPT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            2..1351
                     /gene="ccp"
                     /locus_tag="OQM47_004049"
     CDS             2..1351
                     /gene="ccp"
                     /locus_tag="OQM47_004049"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1481971.1"
                     /translation="MCYLGLSGYVWYHDNKRSKQADVQASAVSENNKVLGFLREKGCD
                     YCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAALLADKPVSQSDLNKIEW
                     VMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYASNDTAPEHRNEPVQPI
                     PQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGAVGPI
                     NAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQ
                     FLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNK
                     CATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVA
                     LTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1562..>2046
                     /locus_tag="OQM47_004050"
     CDS             1562..>2046
                     /locus_tag="OQM47_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1481972.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
BASE COUNT          557 a          426 c          528 g          535 t
ORIGIN      
        1 gatttgctat ttagggttat ctggttatgt gtggtaccac gataataaac gcagtaaaca
       61 ggccgatgtt caggcatctg ctgtcagtga aaataataag gttttaggct ttctccgcga
      121 aaaaggatgc gactattgcc acacgccttc ggcagaatta cccgcctatt attatattcc
      181 tggcgcgaaa cagttgatgg attacgacat taagcttgga tataaatctt ttaacctcga
      241 ggccgtgcgt gcggcactgc tggctgataa acccgtttcg caaagcgatc tgaataagat
      301 tgaatgggtg atgcagtatg aaactatgcc accaacgcgt tataccgcgc tacactgggc
      361 gggtaaggtg agtgatgaag agcgggcgga aatactggcc tggattgcaa aacagcgcgc
      421 ggaatattac gccagcaatg atactgctcc ggagcatcgc aatgaaccgg tgcagcccat
      481 cccgcaaaaa ctgcctaccg atgcgcaaaa agtggcgttg ggctttgcgc tgtatcacga
      541 tccccgttta tcggctgata gcaccatttc atgcgctcat tgccatacgt tgaatgcggg
      601 gggcgtcgat ggcagaaaaa catcgattgg tgttggtggc gcagttgggc cgattaatgc
      661 gccgacggta tttaactcag tatttaacgt tgagcagttc tgggatggtc gtgcggcaac
      721 attgcaggat caggcaggtg gaccgccgtt gaacccgatt gaaatggcgt cgaaatcctg
      781 ggacgaaatt attgctaagc tggaaaaaga tccgcagctt aaagcgcagt tcctcgaagt
      841 ttatccgcaa ggtttcagtg gcgaaaatat tactgatgcc attgctgaat ttgagaaaac
      901 attaattacg ccggattccc catttgataa atggttgcgt ggagatgaaa atgcgctgac
      961 ggcgcaacag aaaaaaggct atcaattatt taaagataat aaatgtgcaa cttgtcatgg
     1021 tggtattatt ctcggcggac gttcctttga accgttgggg ctgaaaaaag actttaactt
     1081 tggggaaatt acggcggcgg atattggtcg tatgaatgtg actaaagaag agcgtgataa
     1141 attgcgtcag aaagtacccg gtttacgtaa cgttgcttta acggcaccgt acttccatcg
     1201 cggtgacgtg ccgacgctgg acggggcggt gaaactgatg ctgcgctatc aggtaggcaa
     1261 agagctgccg caggaggatg tggatgatat cgtagctttc ctgcacagtc tgaacggggt
     1321 gtacacgccg tatatgcagg ataaacaata attaatttga tcgcccgaac aggaatgttt
     1381 gggcgatttt tattacgata ataaagtctg tttttaatat tatcatgtta aatgtttata
     1441 ttataaaaag tcgtttttct gcttaggatt ttgttattta aattaagcct gtaatgcctt
     1501 gcttccattg cggataaatc ctactttttt attgccttca aataaattta aggagttcga
     1561 aatggaccag aagctgttaa cggatttccg ctcagaacta ctcgattcac gttttggcgc
     1621 aaaggccatt tctactatcg cggagtcaaa acgatttccg ctgcacgaaa tgcgcgatga
     1681 tgtcgcattc cagattatca atgatgaatt atatcttgat ggcaacgctc gtcagaacct
     1741 agccactttc tgccagacct gggacgacga aaacgtccat aaattgatgg atttgtcgat
     1801 caataaaaac tggatcgaca aagaagaata tccgcaatcc gcagccatcg acctgcgttg
     1861 cgtaaatatg gttgccgatc tgtggcatgc gcctgcgccg aaaaatggtc aggccgttgg
     1921 caccaacacc attggttctt ccgaggcctg tatgctcggc gggatggcga tgaaatggcg
     1981 ttggcgcaag cgtatggaag ctgcaggcaa accaacggat aaaccaaacc tagtgtgcgg
     2041 tccggt
//
LOCUS       DAJVPT010000239         2022 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVPT010000239 DAJVPT010000000
VERSION     DAJVPT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(30..1313)
                     /locus_tag="OQM47_004051"
     CDS             complement(30..1313)
                     /locus_tag="OQM47_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1481973.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(1448..>2022)
                     /locus_tag="OQM47_004052"
     CDS             complement(1448..>2022)
                     /locus_tag="OQM47_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1481974.1"
                     /translation="ATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTG
                     QRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGG
                     ETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKF
                     AQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT          485 a          515 c          486 g          536 t
ORIGIN      
        1 cgccgcatcc ggcaaatagt taatagctct tacttcttct cctctgcaac cactttgctg
       61 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
      121 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
      181 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
      241 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
      301 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
      361 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
      421 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
      481 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
      541 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacttgg
      601 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacgct atcgcccagc
      661 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
      721 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
      781 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
      841 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
      901 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
      961 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     1021 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     1081 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     1141 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     1201 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     1261 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     1321 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     1381 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     1441 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     1501 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     1561 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     1621 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     1681 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     1741 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     1801 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     1861 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     1921 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     1981 gtcttgatct ccttacctct gattttgctg cgcgagtggc ag
//
LOCUS       DAJVPT010000240         2000 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVPT010000240 DAJVPT010000000
VERSION     DAJVPT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1211
                     /gene="wcaC"
                     /locus_tag="OQM47_004053"
     CDS             <1..1211
                     /gene="wcaC"
                     /locus_tag="OQM47_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1481975.1"
                     /translation="LQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGKESV
                     SHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHVLHS
                     YWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCPTLN
                     NYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIINNGI
                     DMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHTFGK
                     FSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVIATH
                     SDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQQML
                     EEYVNFYQNL"
     gene            1186..>2000
                     /gene="wcaD"
                     /locus_tag="OQM47_004054"
     CDS             1186..>2000
                     /gene="wcaD"
                     /locus_tag="OQM47_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1481976.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
BASE COUNT          468 a          471 c          533 g          528 t
ORIGIN      
        1 ttctgcaatt taatgtgcga ctggcggaag gcggggcagc aggtgtggcg ttagatctcc
       61 accagcgtgc gctgcaacag gggctggcgt cacattttgt ctacggctac ggcaaaggcg
      121 gcaaagagag cgtcagccat cagaactatc cgcaggtcat caaacatacg ccgcggatga
      181 ccgcgatggc gaacattgcg ttgtttcgtc tgtttaatcg cgatctgttc ggcaatttca
      241 atgagttata tcgcaccatt actcgcacac cgggtccggt ggtcctgcat tttcatgtgc
      301 tgcacagcta ctggttgaat cttaacagcg tggtgcgctt ttgcgaaaaa gtgaaaaacc
      361 ataaaccgga tgtcaccctg gtctggacgc tgcacgacca ctggagtgta accggacgct
      421 gcgcctttac cgacggttgc gaaggctgga aaacgggctg ccagaaatgc ccgaccttaa
      481 ataactatcc gccggtgaag attgatcgcg cgcaccagct ggtggcgggc aaacgccagt
      541 tattccgcga gatgctggcg ctgggctgtc agtttatttc ccccagccag catgtggctg
      601 acgctttcaa tagtctgtac gggccagggc gttgccggat tatcaataat ggcattgata
      661 tggcaaccga agcgattctg gcagatctgc ctccggtgcg cgaaactcag ggcaagccga
      721 aaatcgcagt ggtggcgcat gacctgcgtt acgacggcaa aactaaccag caactggtgc
      781 gtgagatgat ggcgctgggc gacaaaatcg aactgcatac ctttggtaag ttctcgccgt
      841 tcaccgctgg caacgtggtt aatcacggct ttgaaactga caagcgcaag ttgatgagcg
      901 cgctcaatca gatggatgcg ctggtgttca gttctcgtgt cgataactac ccgctgattt
      961 tgtgtgaggc gctatcgatt ggcgtgccgg tgattgccac ccatagcgat gcggcgcggg
     1021 aagtgctgca aaaatccggt ggtaaaaccg tcagtgaaga agaggtgctg caactggtac
     1081 agttaagcaa accggaaatc gcgcaggcga tatttggtac cacgctggct gagttcagcc
     1141 aacgcagtcg cgccgcctac agtggacaac agatgctgga ggagtatgtc aacttctatc
     1201 agaatctgta gctacctgct gctgccactg atctacctgc tggttaacgt caaaatcgcc
     1261 cagcttggcg aaagtttccc catcaccatc gtcacttttt tacctgtctt gttactgctg
     1321 tttttagaac gcatcagcat taaaaaattg atgattgcct tagggattgg cgcgggactc
     1381 acggcgttta actacctgtt tggtcagtcg ttggatgccg gtaaatacgt cacttcaact
     1441 atgctgtttg tctatatttt gatcattatt ggcatggtgt ggagtattcg ttttaaaaca
     1501 atttcgccac acaaccatcg taagatatta cgtttctttt atctggtggt cggactggtg
     1561 gtggcgctgg cggcggtgga gatggcgcaa attatcctca ccggtggcag cagtattatg
     1621 gagtcgattt cgaaatatct gatttacagc aacagctatg tgctgaattt cattaaattc
     1681 ggcggcaagc gcacgacagc actttatttc gaaccggcat ttttcgctct ggcattaatc
     1741 tcaatttggc tcagcatcaa acagtttggt atcaaaacgc ctaaaacaga tgccctgatt
     1801 ctcgcaggga taatattatc cggatcgttt tcaggggtta tgacctttat cctgttttat
     1861 ttgctggagt gggcatttca atatctgaat aaagaggcga ttaagaaaaa attaccgtta
     1921 gcattgattt ctctggctgt attccgggtt ggtgtggtaa tcgcgtttcc ttatatttcc
     1981 acccgtctgg gcgatttagg
//
LOCUS       DAJVPT010000241         1962 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVPT010000241 DAJVPT010000000
VERSION     DAJVPT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..168)
                     /locus_tag="OQM47_004055"
     CDS             complement(<1..168)
                     /locus_tag="OQM47_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481977.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFS"
     gene            complement(385..918)
                     /locus_tag="OQM47_004056"
     CDS             complement(385..918)
                     /locus_tag="OQM47_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1481978.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(984..1613)
                     /locus_tag="OQM47_004057"
     CDS             complement(984..1613)
                     /locus_tag="OQM47_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481979.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1617..1832)
                     /locus_tag="OQM47_004058"
     CDS             complement(1617..1832)
                     /locus_tag="OQM47_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1481980.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          492 a          526 c          437 g          507 t
ORIGIN      
        1 cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg cccatgccgg
       61 aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg agcacccgac
      121 caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca gctaactcct
      181 tcgtgtattt agcatcgagt gcatcagcat cacgctggcg ctgctgcatg tcagtaatgg
      241 ttgcgttcgc cagcttcagt tctccggcgt ttttgtcgcg ctgctctttg taggtaatgg
      301 cgttatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag ataaccagag
      361 cggagataat cgcggtgact ctgctcattg atctatcccc caacaggcta atgcgctttc
      421 ctggtcacga cgaataacct gtccatagca gttatttgaa cgtatgcggc aatcgcgccc
      481 aacatctttt atccaccagc gaatcgcctc gcatgcgccc ttacgatcac cggcattcag
      541 ccgcttataa aacgtcgacg ggaaacactt accggggcca atgttatagg ggcaaaatga
      601 cgctataccc gctttctggg gttcggtcag aggtacttta atattgcgct ccacccatgc
      661 cagcgcctta tcacgttcaa tagcgttaac ctggtcacat ttttccttcg acagcttcat
      721 tcccggtatg acaggcttac catccaccat tgtggcacca cgacagatgg tccatatgcc
      781 agaaccatca cggtatgccg ttgtgtgatt accctctttt tcgttcagaa actggtcaag
      841 tatctgggga gcagacgcgc ctgcggcaat cagcgccaga acagcagccg acaggccgta
      901 tttgattttt gcgctcatgg atatttatca ggatttatcg gcaacaaata acgagccaac
      961 ttatatacgc cttttaagat aagtcagccc cggctggatc cagtcatcag gctctttctt
     1021 aaagggggag tattgaaatc acgaagaaga gcctcccgca ccgcagcatc catgtctgca
     1081 ccactggcaa gtgcttcaat ctctgccgcc acctgcaaat accccatgca acggccaatg
     1141 cgtttcataa ctctggtgtt ttcgtctttc aggcatctga tagcgagatt aataattttc
     1201 aggcagtgat gatgcgatgc ccggacaggt tttccgttct cataaacgac gaactcaccc
     1261 tcttcacagg aaaccctgaa aggccgatgg ggttcctggt ctttatccgg tgcagacgca
     1321 ataaaatcac ttttatgctc ttcctctccg gcactgttaa ccgtaatgac gtactcaggc
     1381 cgcaggtaag ccgcctcttt ttctgacaat gcggccggta aaaacttttc ctgcaaaaac
     1441 ttcataaaag tctcgtgagc gactttgtgg cagtaatcgt aaatcgccgc ataatgctca
     1501 tcgcggcgtt tgttttcatc ttcagaactc accaacgccg acaatttttt attaagctca
     1561 gtgatttcat tttccaggtg attgaatcgc tgattcattt cttcatgctt cattatttac
     1621 tctccccgtg cagccttacg cttgtcttct cttattttga aatacagatt cgtcagataa
     1681 gtcagaagcc ccagaagcag acttcccagc acaccaatcg cagcccactg tgatggcctg
     1741 acctgatcca accactgcaa aaaccagtag ccggcactgc cggcggaggt gccgtaggca
     1801 atgcccgttg aaattttgtc catggatttc atagcctcac ctccgcaaat aacggatggc
     1861 gtagttttac actgagaaat gaaagggatt tgaaaagaaa aaaacgcaaa agcgggcgaa
     1921 acgatatata cagtaaggaa agcactctat ccaacaaacc ac
//
LOCUS       DAJVPT010000242         1955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVPT010000242 DAJVPT010000000
VERSION     DAJVPT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(238..1842)
                     /locus_tag="OQM47_004059"
     CDS             complement(238..1842)
                     /locus_tag="OQM47_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1481981.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          487 a          488 c          444 g          536 t
ORIGIN      
        1 ccagagcaca caggaagcat tcactttaag tagaacgctt ggtgtctgta attggtcatc
       61 ccgcccctga aaggatgcga taagttacgg ttagttgtcc tagaagagaa aactttttgt
      121 gtcccttctc cctatagtga gtcgtattaa ttatcccttg acaagtagag aaaactatga
      181 gtcaaaaaca tgaccacaca ggtctgtagt aagtgtcatc ctagactatg gtctcctcta
      241 ggtctcctta taaacatcag ccatcacatt gaccagccta tcaacctcga cctctttagc
      301 ataacggtcg tacgtaatag accctgaact atgccccatg atcgcctgtg catacgccaa
      361 ctcgacccca tgttgtttta actgggccgc aactgtatgt ctaaacgagt gaaacgctaa
      421 gccgctacgg tctacgagga ctttaggcaa caacgtccga ttaaaccatt tactcacctg
      481 ctcaccgtaa ccgttcttca tgagacgcag accatcgaac accatagcgt catcaccgtc
      541 agcgcctcta cgggtctcta caagcgccat aaagtcagcc aatataaaac cataggcccc
      601 atcgaccaga ggcacacagc ggtcactatg agcgttcttg atgctcttac cgggtagact
      661 actatcgtct tcattgatat gaatataaac ggttcccgcc tcagtggtcc tgacgtcttt
      721 gacctgtagt tgggctattt cattgagtct cgccccagtg atagcagcca aggcggtaac
      781 ataataatga tacggcttac cagaggtctt ctgagagtat gccttagctg cgaccagtag
      841 ttgccccacc tgctccgtag agaaggcgtt acgggcccct gaggctttac gctgagggac
      901 tttaagctcc agaccctcag tcatgttctt tttaatcaag tcattgcgca cagcccacct
      961 aaagacagca gccatcttaa taagatactt gttgttgatg gtaacgacat ccagacaatc
     1021 tgtcttatct tcacggctca acaggtcagc aagtgggaca tccttgaagc gctgcttacg
     1081 gttcctaggt aactgctgga gcacatcacg gaccctaagc atgtcagccc tgttagcgtc
     1141 tgcattgaga cccagatggt cgaatatttc gattaacgca gcgtgtgacg ccttgttttc
     1201 ccttaaggtt gcaggcttcc agttctgata gttctccgct tcgtattgtt caaagaggct
     1261 cgctatggta atgctaggcg taacttctgg ttttacttct ggagcattaa cagataggga
     1321 cacagagtct gcttggttat cttgcctatc aatatcatta aaaagatcga cgtaatctat
     1381 cagaggctga ctgtctcccg cttcgatacg cctcatacag gcttttagca ccttcagggc
     1441 ctcattgatg taacgatgct ggtcaatgct tagcggctca ctagccagag catcggtaag
     1501 gctctcacca agttctccgt attggtcccg gtagatatcc ctcatgtcag gctcaaagag
     1561 atctgaacgg cccatagaca gttcacactc agcgatgtcc ttaaggtgtt ccctcatttc
     1621 ctcataggta gcgttgggtc tgtctgcgtg tattgccttg agtgctgcct ttatgtgcct
     1681 tgagtaagcc atagcggtgc gtctatcctt ggtccgtaat gatattgagg tcattctatc
     1741 attgtttgat tgtctgaccc taagacggaa gtagtagacg ccgttacgct ggtagagata
     1801 gtagactgct ggaagtttgt gactactgga tagctgaatc atatgtctgc atgccctgag
     1861 agggttacac agtgggttac agtaagtaac tctgcaagga ggcttaaagc cgtatccctt
     1921 aggattcagt aggttggagt actttaagaa aggtc
//
LOCUS       DAJVPT010000243         1939 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVPT010000243 DAJVPT010000000
VERSION     DAJVPT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            144..287
                     /locus_tag="OQM47_004060"
                     /pseudo
     CDS             144..287
                     /locus_tag="OQM47_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            434..700
                     /gene="ospE1"
                     /locus_tag="OQM47_004061"
     CDS             434..700
                     /gene="ospE1"
                     /locus_tag="OQM47_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCS1481982.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1091..>1939
                     /gene="ipaH1.4"
                     /locus_tag="OQM47_004062"
     CDS             1091..>1939
                     /gene="ipaH1.4"
                     /locus_tag="OQM47_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCS1481983.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          661 a          413 c          322 g          543 t
ORIGIN      
        1 catcccgtat tgggcagtat gatgccggaa agctgcgagg ctccacacct gccagtttca
       61 cccttttaac catcatacta ctccgtgttt aattgtgtgg gatgcgttca ttacatttta
      121 acagtgtcac cccgtcggcc acgaaagata aacaggtgac cagaaaaggg atcttcatcc
      181 agcacatgct atatcttgaa cgtaacaacc tgaccctccg cacccgcatc aagcggctgg
      241 cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt
      301 cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat cgttttttta
      361 cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc aaaatataac
      421 ataaggaagt aatatgctta cacaaactat attcccttgt cttccacaaa aacaagaaaa
      481 tatcatcctt gaagtatcaa atcctgtatt attatcttct actgttacta cagatggata
      541 tactgttttc aataaaaaag cagctatata cgaattacaa atacctgctg ccagccggac
      601 gaaaacgttg aaattcactg ctacagaaat gcaatggttg acaaagataa atgaagccgg
      661 aattgatgaa aaacaaagcc aacggtattc tgatttctga agttaatttc agactcgttt
      721 aagctccatt ttcaaaaaaa atatatcagg gtttgttact aataacttct gaaaacagaa
      781 aaaaaataga gcagtaattc tatttcagag aggagtaact ccatctatcc tcgatacgta
      841 ctgaacgtta tcgtgatata ttcatcatgc tttcttcacc attattcgag tatagggaga
      901 gacgcaatga ccttaaatac ctttttatgg ctgaattacc attctcagga ataacagcag
      961 actccttccc atagacggct tagggggaaa gcaatacccg gagaaagggt acaaagcaaa
     1021 ctgttttaaa attaataagc catattctat ttaactagaa ttcttatcgt tgataagaat
     1081 tctagttata atgattaaat caaccaatat acaggcaatc ggttctggca ttatgcatca
     1141 aataaacaat gtatactcgt taactccatt atctttacct atggaactga ctccatcttg
     1201 taatgaattt tatttaaaaa cctggagcga atgggaaaag aacggtaccc caggcgagca
     1261 acgcaatatc gccttcaata ggctgaaaat atgtttacaa aatcaagagg cagaattaaa
     1321 tttatctgag ttagatttaa aaacattacc agatttaccg cctcagataa caacactgga
     1381 aataagaaaa aacctattaa cacatctccc tgatttacca ccaatgctta aggtaataca
     1441 tgctcaattt aatcaactgg aaagcttacc tgccttaccc gagacgttag aagagcttaa
     1501 tgcgggtgat aacaagataa aagaattacc atttcttcct gaaaatctaa ctcatttacg
     1561 ggttcataat aaccgattgc atattctgcc actattgcca ccggaactaa aattactggt
     1621 agtttctgga aacagattag acagcattcc cccctttcca gataagcttg aagggctggc
     1681 tctggctaat aattttatag aacaactacc ggaattacct tttagtatga acagggctgt
     1741 gctaatgaat aataatctga caacacttcc ggaaagtgtc ctgagattag ctcagaatgc
     1801 cttcgtaaat gttgcaggta atccattgtc tggccatacc atgcgtacac tacaacaaat
     1861 aaccaccgga ccagattatt ctggtcctca aatatttttc tctatgggaa attctgccac
     1921 aatttccgct ccagaacac
//
LOCUS       DAJVPT010000244         1935 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVPT010000244 DAJVPT010000000
VERSION     DAJVPT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..685
                     /locus_tag="OQM47_004063"
     CDS             <1..685
                     /locus_tag="OQM47_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1481984.1"
                     /translation="TLAMIDEVHQRPEGTARAAFNRNREHFISGVDYAELGADVIRTD
                     LPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQVQRELVNSYFRTRTPLTEIEMIA
                     AIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMRAGYVGYRQVVAKSGMSDTKCRN
                     LVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAFHQMMSEADPRGTRWYHPKMGLF
                     QVIGWEDKA"
     gene            682..1251
                     /locus_tag="OQM47_004064"
     CDS             682..1251
                     /locus_tag="OQM47_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481985.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            1375..1617
                     /locus_tag="OQM47_004065"
     CDS             1375..1617
                     /locus_tag="OQM47_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1481986.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            1621..1767
                     /locus_tag="OQM47_004066"
     CDS             1621..1767
                     /locus_tag="OQM47_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481987.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            1776..>1935
                     /gene="xisR"
                     /locus_tag="OQM47_004067"
     CDS             1776..>1935
                     /gene="xisR"
                     /locus_tag="OQM47_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCS1481988.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          510 a          456 c          521 g          448 t
ORIGIN      
        1 gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc gtgcagcatt
       61 caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag gtgcggacgt
      121 aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg ggattgtact
      181 ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg cctggcaggt
      241 tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg aaatcgagat
      301 gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc atgtggaaga
      361 acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata tgcgcgccgg
      421 atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca agtgtcggaa
      481 tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc cggacggtct
      541 gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc accaaatgat
      601 gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc ttttccaggt
      661 gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc cgctctggtg
      721 tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac gccgaaacat
      781 attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg gacgaagaaa
      841 aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac agagctgatt
      901 tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact gtccattacc
      961 ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc gaaaaagttt
     1021 gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga gaagatgttt
     1081 ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg gcaagcggtc
     1141 agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt tgttgtgatg
     1201 ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg aaaatcgaat
     1261 accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta actctgggga
     1321 tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa ccacatgttc
     1381 gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt agaaattact
     1441 cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg gcgagtacgc
     1501 atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca caagattctg
     1561 gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag gaagtaaaaa
     1621 atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta cgacctgacg
     1681 cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga ggtctggtgc
     1741 cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt caactggctc
     1801 ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa ccaggcacaa
     1861 ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac gtatcaccag
     1921 acggtaatcc aaagc
//
LOCUS       DAJVPT010000245         1834 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVPT010000245 DAJVPT010000000
VERSION     DAJVPT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..209
                     /locus_tag="OQM47_004068"
     CDS             <1..209
                     /locus_tag="OQM47_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1481989.1"
                     /translation="LNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASG
                     GKNVKWAAINDYGGSSGTETRPLQ"
     gene            238..>1834
                     /locus_tag="OQM47_004069"
     CDS             238..>1834
                     /locus_tag="OQM47_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1481990.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSN"
BASE COUNT          550 a          441 c          404 g          439 t
ORIGIN      
        1 cactcaatat atataaccca actgaatatt acgtcgtatt tgccggactg gcagtcgata
       61 aaaccgatct cacaagcaaa attgaatata tcgcgcccgg agaacataaa cagttaccac
      121 ttccagcatc tggcggaaag aacgtgaaat gggctgcgat caacgattat ggcggcagtt
      181 ccgggacaga aactcgtcca ctgcaataaa aaatataaaa acacaggcca tcagggaatg
      241 ccacaacgac accaccaggg acataaacgc acaccgaaac agttggcgct cattatcaaa
      301 cgttgtttgc cgatggtgct cactggcagc ggcatgcttt gcactaccgc taacgccgaa
      361 gagtattatt tcgaccccat tatgctggaa actacaaaaa gtggtatgca aacaaccgat
      421 ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt tgatatctgg
      481 ctgaataaaa agaaggtttc acagaaaaaa attacattta ccgccaatgc agagcaactt
      541 ctgcagccac agtttacggt agaacaacta cgtgagctgg gtattaaggt ggatgaaatc
      601 ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca aatcattccc
      661 ggtacagctg ctgaatttga tttcaatcat cagcgactta atttgagcat tccccaaatt
      721 gcactgtacc gtgatgcaag aggttacgtc tccccttctc gttgggacga tggtatacca
      781 acgctgttta ccaactactc gtttacaggt tctgataacc gttaccgcca gggcaatcgt
      841 agccaacgac agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg gcgattacgt
      901 aactattcta cgtggacacg caacgatcag gcgtcaagct ggaacactat cagtagttat
      961 ttacaacgtg atatcaaggc gttgaagtct cagttgcttc tgggagaaag cgccaccagc
     1021 ggcagtattt tttccagcta cgcctttact ggcgtgcaac tcgcttccga cgataatatg
     1081 ttgccaaaca gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa cagtagtgca
     1141 atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc agcgggtgcc
     1201 tttgaaatta acgatctcta cccctcttcc aacagcggcg atttagaagt cacgattgaa
     1261 gaaagtgacg gtacacaacg tcgctttatc cagccttatt cttcattacc catgatgcag
     1321 cgacctgggc atctaaaata tagcgcgacc gctggacgct atcgcgctga tgcaaacagt
     1381 gatagcaagg aacccgaatt tgctgaagcc acggcaatat atggtttgaa taatactttt
     1441 acgctgtatg gcggcctgct cggttctgaa gattattatg cgctggggat cggtatcggc
     1501 ggcacacttg gcgcactggg cgcgttgtcg atggatatca acagagctga cacccaattc
     1561 gataaccagc actcttttca tggctatcaa tggcgtactc agtacatcaa agatatcccg
     1621 gaaaccaaca ccaatatcgc tgttagctac tatcgctata ccaacgatgg ctattttagt
     1681 tttgatgaag ccaatacccg caattggggc tataacagtc gccaaaaaag tgaaattcaa
     1741 ttcaacatca gccagacaat atttgatggg gtaagtctgt atgcctccgg ttcacagcaa
     1801 gactattggg gcaataacga gaaaaacagt aata
//
LOCUS       DAJVPT010000246         1801 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVPT010000246 DAJVPT010000000
VERSION     DAJVPT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..209
                     /locus_tag="OQM47_004070"
     CDS             <1..209
                     /locus_tag="OQM47_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCS1481991.1"
                     /translation="ETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAIIF
                     IRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(263..868)
                     /gene="azoR"
                     /locus_tag="OQM47_004071"
     CDS             complement(263..868)
                     /gene="azoR"
                     /locus_tag="OQM47_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1481992.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1069..>1801
                     /locus_tag="OQM47_004072"
     CDS             1069..>1801
                     /locus_tag="OQM47_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1481993.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNA"
BASE COUNT          469 a          434 c          470 g          428 t
ORIGIN      
        1 tggaaacatt acgcgaagag caaggcagcg ttctggtcca ttgcgcattg ggattatcgc
       61 gcagtgcgct ggtagtggcg gcatggctgt tatgttacgg acactgtaaa acagttgatg
      121 aagcgattat ctttattcga gccagacgct cgcatattgt gcttaaggaa gagcacaaag
      181 cgatgttgaa attatgggaa aacaggtaag tggattgaga tgtggactga atatctacag
      241 tccacatcaa gaccgtgtcc ggttatgcag caacaatgct gtcgatggct gcttttgcgt
      301 cagactgtgc tttcgctgcc atttccggac cgtatgcgat cccttcggcg aagacaaatt
      361 tcacatcggt aatgccgata aagccgagga acgtggacag atacggcgtc accaggtccg
      421 ttggtccatc tttgtggatc ccaccgcggc tggtaataac gatggctttt ttacccgtta
      481 ccagaccttc cggaccgttc tcggtatagc ggaaagtaac gcctgcgcgt gcaaccaggt
      541 caaaatagtt tttcaactga gttgagatgt tgaagttata catcggtgcc gcaataacga
      601 taacgtcgtg ggctttcagc tcggcaatca gctcatcgga aagcgccaga gcttcctgct
      661 gacgcggagt cagcggcgca tcgctcggac gcagagcgcc aaccagttcg ccatccagta
      721 ccggaatcgg atttgcagct aggtcgcgaa cggtgatttc atcagcggag tgcttttcgc
      781 tccattgttc aacaaaataa tcggacaact gattagactg agagtaccct gccaggatgc
      841 tggatttaag aactaatacc ttgctcatgg tgtttcctta tagatgtttg aatggacgat
      901 gccccgttgc ttgctgacac tttattcaca atcctgccac agagatcgcg caataaatcg
      961 aagtctatgt tcgaatttat tgaacaacgc atagaaagcc gcgatgtggt actctatatc
     1021 tatcatttaa aagaaaatta atcaggcaga ctactgccca ctaacgttat gacagaacaa
     1081 caaaaattga cctttactgc cttgcaacag cggctggatt cgctgatgct gcgcgacaga
     1141 ctgcgttttt ctcgccgtct gcattgtgtt aagaaggtta aaaatcctga tgcacaacag
     1201 gccattttcc aggagatggc gaaagagatt gaccaggcgg cagggaaagt cctgctgcgt
     1261 gaagcggcac gaccggaaat cacttatcct gacaatttac cggttagtca gaaaaaacag
     1321 gacatactcg aagcgattcg tgatcaccag gtggtgatcg tcgccgggga aaccggctcc
     1381 ggtaaaacga ctcagctacc gaaaatctgt atggagctgg ggcgcggtat taaagggctg
     1441 atcggtcata cccagccgcg tcgcctggcg gcacgaacgg tggccaaccg tattgcggaa
     1501 gagctgaaaa cggagccggg cggttgcatc ggttacaaag tgcgtttcag cgatcacgta
     1561 agtgataaca cgatggtcaa gctgatgacc gacgggatcc tgctggcgga gatccagcaa
     1621 gaccgcctgc tgatgcagta cgacaccatc attattgacg aagcgcacga acgcagcctg
     1681 aatatcgatt ttctgctcgg ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa
     1741 atcattatca cttccgcgac tatcgacccg gaacgctttt cgcgccactt taataatgcg
     1801 c
//
LOCUS       DAJVPT010000247         1714 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVPT010000247 DAJVPT010000000
VERSION     DAJVPT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(96..497)
                     /locus_tag="OQM47_004073"
                     /pseudo
     CDS             complement(96..497)
                     /locus_tag="OQM47_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            926..1714
                     /gene="virF"
                     /locus_tag="OQM47_004074"
     CDS             926..1714
                     /gene="virF"
                     /locus_tag="OQM47_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1481994.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          531 a          319 c          322 g          542 t
ORIGIN      
        1 ttttttcgta agcgatgaga tgtggagcca gaaaaatggc tgcgttacgt cattgaactg
       61 tccttgaccc acgatattca gacagtcgtt gtatcttaag ttgacattac ccgctgacgt
      121 cgatattccc ttggtgagag ataacccaac gcactatgcg gatggttttc attgtaatgc
      181 tcgatcgcca ctgcaaggtt atgcaatgcc gttcttggtt tcggcatgaa cgcgatgcag
      241 tcttccttca tcgttttcat gaaccgttct gctattccat tactctgagg gctactgact
      301 gccgtcgtgc agggctccag attcaactct ctggcgaact gccgtgtttc atgagccgta
      361 taagccgaac cgttatcggc cactggattg gccgttccgg tcctttatcg ccatatcgct
      421 cctgacatga catcctgcac cgttgctttg tcataatctc ccgtgcctgc tgcccagtct
      481 atggcttcac gatcgcaata tggaacgtaa cgctgtcatc gggagaatcg tcaatgcggc
      541 attcggttaa cgcttaccaa tatcaacacc aaccggattt gtggagcctc cagtctgaag
      601 gggctttatg cgttccgtat aggatattat gatgctggag ttttgcgaag ctccactcct
      661 ttccgaacat ttacacagat attgcctaag aaaagtgaaa ccataatatg gttatagtcc
      721 ctttcagtgc aaatacttag cttgttgcac agagaaatag aagctgcata agctctttct
      781 tcaaaaaatg taaataaagt taaatatagg aaaaattact taatctatct taataacgga
      841 aagattttgt atacaatcac tgttacagaa atttcttagt tactctgtaa acactaaata
      901 tagtttggta tattctgttg aatttatgat ggatatggga cataaaaaca aaatagatat
      961 aaaggttcgc ttgcataact atattatttt atatgcaaaa aggtgttcaa tgacggttag
     1021 ctcaggcaat gaaactttga ctatcgatga agggcaaatt gcttttatag agcgaaatat
     1081 acaaataaac gtctccataa aaaaatctga tagcattaat ccatttgaga ttataagcct
     1141 tgacagaaat ttattattaa gcattattag aataatggaa ccaatttatt catttcaaca
     1201 ctcctattct gaggagaaaa gggggttaaa caaaaaaata ttcctcctct ctgaggagga
     1261 ggtttctatc gatttgttca aatctataaa agagatgcct ttcggcaaaa gaaagatcta
     1321 tagtttagct tgccttttat cagctgtttc tgatgaggaa gctttatata cttcgatatc
     1381 gatagcttct tctcttagtt tttctgatca gataaggaag attgttgaaa aaaacatcga
     1441 gaagagatgg cgtctttctg atatttcaaa taacttgaat ttatcagaaa tagctgttag
     1501 aaaacgattg gagagtgaaa aattaacatt tcaacaaatc cttcttgata ttcgcatgca
     1561 tcatgcagca aagcttttat tgaatagtca aagctatatt aatgatgtat caagacttat
     1621 cggaatatca agcccatctt attttataag gaaatttaat gaatattatg gtataactcc
     1681 aaagaaattt tacttatatc ataaaaaatt ttaa
//
LOCUS       DAJVPT010000248         1703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVPT010000248 DAJVPT010000000
VERSION     DAJVPT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(854..1327)
                     /locus_tag="OQM47_004075"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="OQM47_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="OQM47_004076"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="OQM47_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          474 c          413 g          437 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aag
//
LOCUS       DAJVPT010000249         1703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVPT010000249 DAJVPT010000000
VERSION     DAJVPT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..683)
                     /locus_tag="OQM47_004077"
     CDS             complement(<1..683)
                     /locus_tag="OQM47_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCS1481995.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGL"
     gene            815..>1703
                     /gene="apbC"
                     /locus_tag="OQM47_004078"
     CDS             815..>1703
                     /gene="apbC"
                     /locus_tag="OQM47_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1481996.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTG"
BASE COUNT          412 a          418 c          472 g          401 t
ORIGIN      
        1 tgcaggccgg attcaaacca ctcctgcatt ttgtttgcca cctgctcctg caacgcaccg
       61 ctgcgggtcc atgcctgcag catttcgctg aaagagggca gatcaaagaa gaagtgttca
      121 gaatcacgca tcactggtgt agcaccagaa accaccgatt tcggctcgat caattcagtc
      181 gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg ggatttacat
      241 ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc cggatcgtac
      301 agctgagaga tggtgcggtt tttaataaaa ccgttttctt tcaggcgaga gtagataagt
      361 tctgacaact ggcggttctc ttcgctgtgc gtcgagtgat agttgtcata gctgatgtta
      421 aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg ctccggggtg
      481 ataccgagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc ggcgcagatg
      541 aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc ctggatgtgc
      601 tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca cgtcaccaga
      661 attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa aggggttaga
      721 tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg taaactatca
      781 gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc aggccaaatc
      841 accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc acccaaccct
      901 gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc gcctggatgg acgacacgtt
      961 gcatgtggaa ctggttatgc cgtttgtctg gcatagcgcg ttcgaagagt taaaagagca
     1021 atgtagcgcc gaactgctgc gcatcaccgg ggcgaaagct atcgactgga aactgtcgca
     1081 taacatagcc actctgaaac gcgtgaagaa tcagccggga attaacggtg tgaagaacat
     1141 tatcgcggtg agttccggta aaggcggcgt gggtaaatcg tccacggcgg taaacctggc
     1201 gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata tctacggccc
     1261 atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg acggtaccca
     1321 catggcaccg attatgtctc atggcctggc aaccaattca attggttatc tggtcactga
     1381 cgacaatgcg atggtgtggc gtggaccgat ggcgggcaag gcgttaatgc agatgttgca
     1441 ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag gcaccggtga
     1501 cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg tcaccacccc
     1561 gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg atgttcgaga aagtcgaggt
     1621 tccggtgctc ggtattgtcg agaacatgag tgtgcatatt tgcagtaact gtggtcacca
     1681 tgaaccgatt ttcggtaccg gtg
//
LOCUS       DAJVPT010000250         1667 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVPT010000250 DAJVPT010000000
VERSION     DAJVPT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            5..751
                     /locus_tag="OQM47_004079"
                     /pseudo
     CDS             5..751
                     /locus_tag="OQM47_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            766..1188
                     /locus_tag="OQM47_004080"
     CDS             766..1188
                     /locus_tag="OQM47_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1481997.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1246..1602
                     /locus_tag="OQM47_004081"
     CDS             1246..1602
                     /locus_tag="OQM47_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1481998.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          459 a          358 c          481 g          369 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggcggcgt tctggaacgc atccgccgac tgaccccgca
       61 acatgtaacc gccccattca gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct
      181 gaatcgctca ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt accaggtgca
      241 gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg aactgatgac
      301 cgggtgtaca aattttgcgt tcagcggaaa acctgatacc gggtagaatc acttagcggc
      361 agctatcggg aatcgcctgc tgaaagacgg tcagacagtg attgtggtta ccgtggctga
      421 tgttatgagc gccctgcacg ccagctatga cgacgggcag tcaggcgaaa aatttttgcg
      481 ggagctgtgc gaagtggatc tgctggttct tgatgaaatt ggcattcagc gcgagacgaa
      541 aaacgagcag gtggtgctgc accagattgt tgatcgccgg acagcgtcga tgcgcagcgt
      601 ggggatgctg acaaacctga actatgaggt gatgaaaaca ttgctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggttaatttt aactgggaga gctggcgtcc
      721 gaatgttagc cattcgaggg ttgttaagta gtttcaggag gatttatggc gaaacctttt
      781 actcccgaac agcgggaaga actgaagacg cgaattgtgg aactcgtgca tcaggacggt
      841 cgggtcacga ttcggcagtt gtccgatgaa acaggtatca gtcgtgcgtc tgtcggtcgc
      901 ttatgcatag aactggtcgc aagtggtgat gtatataatt ctggctacgg cttattcccg
      961 tctgaacagg ctcgcaagga ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg
     1021 aggaaaccgg ttgttgttga tccggacctt atctggtcat tacctgacgg agaaatacgc
     1081 cgctacgaca ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag
     1141 cgtgtactgg ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct
     1201 atcaggcttc aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca
     1261 gaagatgagt taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag
     1321 gttaattttc tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt
     1381 gacgaaaaag agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac
     1441 tggatgctct ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc
     1501 caggaggaac aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg
     1561 aaagcgcgga tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta
     1621 aaaatttaat ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJVPT010000251         1658 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVPT010000251 DAJVPT010000000
VERSION     DAJVPT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            658..1404
                     /locus_tag="OQM47_004082"
     CDS             658..1404
                     /locus_tag="OQM47_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1481999.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT          429 a          427 c          411 g          391 t
ORIGIN      
        1 gcgcggataa ctgaagaacc aagacaccat tactgaggtc tgccatccat ttgtggacag
       61 gcggaaactc aaagaggtta gatcagtggt gtctgaggtt ggagctttga gtcttgacca
      121 gcaggctgga ggagaccatg agtttatctt gttatggatt gtgttatcac aattccaata
      181 agaagaactt taagtccagc ctccactccc gccaacccga agtaaaatta tggtcgctat
      241 gatgtggtct ccatgcttcg ggtcttactg gaagtactga acactttcgc gtcaagttct
      301 ccgaagttca ttcctgtttg actaaactat cactataggg accatatctc gcctctgacc
      361 tcctgagaga cggcaagcga gaaaccgtgg tccacgctag caagctagcc accagcctaa
      421 ggcaagacag cctctggtca gcctgtgtgt tctttggtct cgtgacttaa gtctatcacc
      481 acacacgctc ggacatgctg tcgaaccttc atctctatgg tcatcgcaga ccactaaccc
      541 caattacctc aagtcattct gtttgcgtcc tgttgcctgc tggtctccct ttacgtgggg
      601 gcttgagtag gtcagtgggg tgcgacttca gggtgcaaac aaaaggagta cacgtaaatg
      661 tcccaacaag aaatcagcat cattaacctc gaccagcttg tgtccatgac ctcagttgaa
      721 attgctgagc tgacaggaaa agaacataaa cacgttttac gagatattcg taatatggtc
      781 gaagagctta acggggccaa aactgagcac tgctcaactt tgagcagcga gcttaacggg
      841 tccaaatttg ggctcgttgg cgaggaagta tacaaggacg cgaaaggtga gagccgaaca
      901 atgtatcgtc tcgaccgtaa gcatactttc attctggtcg ctggctattc cgttcacctc
      961 cgcgccaagt gttacgacca tattcagacg ttagagcgtc gagtccttca actggaagac
     1021 cagaagaagc gagcagccat tcagtccgct aaccgccgag gcgtgacttg gggcgactac
     1081 tgcaaaacct atggtctgcc cgctcagaag ctaatgaccg cacttctcca gcatcgtggc
     1141 ctctttcgta agaacccaat cagtaacgag tggtctgtaa atccgaagta ttccgactgc
     1201 tttcgtatca ttaagccgtc agaccagaag ttctcagcag gtgggtataa cttccggttc
     1261 aacgctaagg ggctggaggt cttcggtaag cctgagatgg ttgacaagat gcgaggcatc
     1321 cttattgcat tcactggcac cgaccagcag aagcaagagc acctcctgaa gctggcacaa
     1381 tccggtaagg tggagggaat ctaagatgat gattgacccg cgcacacctg aggggcgcat
     1441 gactctccgt taccgtggct accggactga agtcctcctc agagagcttg gtctagaccc
     1501 ggaagatgag acccgccagc accaatcccg agacgagcta atagcccaac tggtcgctat
     1561 gaaactgccg cttaaccgtt agtagctcac ctactcaata cctaatttaa acaccttttg
     1621 agatgaccga tgcggggaga cccgtaggcc ttactgtg
//
LOCUS       DAJVPT010000252         1643 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVPT010000252 DAJVPT010000000
VERSION     DAJVPT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..292
                     /locus_tag="OQM47_004083"
                     /pseudo
     CDS             1..292
                     /locus_tag="OQM47_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            461..>1643
                     /locus_tag="OQM47_004084"
     CDS             461..>1643
                     /locus_tag="OQM47_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1482000.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          435 a          312 c          364 g          532 t
ORIGIN      
        1 agtggttttg tccgctcaca ccagtacata caggtacgga aataaaacca gtcgaaatgc
       61 cggtaacgac gattactcct gtctctgatg ccggtggact acaagatttt atttactggc
      121 gtcctgatgc ggacagggct ggtatggaac ctgtttatgt gatacttaat gatcctctgg
      181 attccgggcg atttacgcgt aaacaactaa ataaaaatat cttaaacatg caagtaattt
      241 tggtattctc gatacaagaa taaatagtag taactactat agctgcaaat aatgttaaaa
      301 acatttctat gatcatcttt acatttttca tgattgacaa ttgtttttgg ttaaaaaaag
      361 acaactaatg agtgtgataa tgcaatactt tgagtgatta tataatgata agtaaacagg
      421 tttgcgacaa ttggttatca gtattacaga aggattaaat atgtcattat tcacagaacc
      481 taaggaaatt gaaagattac cctctgaaga gattgaacga ttatatcccg tcctgcgata
      541 cagggtcttt atcagcattt tcctcggata tatggggtat tattttgtcc gaaacaccac
      601 cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag atcggcatta tctcctgtgc
      661 cggtttttta tcctatggta tatcaaagtt tgtttccgga cttatttctg acagaagtaa
      721 ttcaaaagta tttttgtctc ttggtttatt cctgtccgga ctggtaaatt ttctgatagg
      781 atatattccc gggatcatca catccgttac gttattttca acaatgtatt tacttaacgg
      841 ctggatccaa ggtatgggat atcctcctgg tgccaaaaca ctggtgttct ggtatgaaca
      901 tcgggaaagg atcacctggg ccacactgtg gaatttatcg cacaatgtcg ggggagctct
      961 ggcccccgtt ctgattggtt tcagttttgg ttttttcggc gacagtgctc tggatcatgc
     1021 ccgggccgca tttatcttcc caggagtact gtgtatggca atgagtgtgc tgatttactt
     1081 tattcaggtg gacagacctg tatcagtggg gcttcctccc attgaagaat ggaaaggtaa
     1141 cgtcgtttca cacccggcta aaggccgtga acaaggtccg agactctcca ttcctgatat
     1201 catcagaaaa catatcatcc gaaataataa actcatttac tgctgtatct atggttcgtt
     1261 tgtttatatt ctgagatacg gtattgtttc ctgggctcct aagtttttaa gtgacagtct
     1321 ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg ggggggggtt ctgtttttga
     1381 aatcggtggt gtggctggta tgcttcttgc cggatacctg tctgtcagac tgttcagaaa
     1441 ctcaaaacca ttaacaaata ctttatttct ggcacttacc attattttat taatcgcgta
     1501 ctggtacgtg ccatcaggca atgaatatct ctggctaaac tatacaatcc ttattctgtt
     1561 ggggctggca gtgtatggtc cggtgatgtt tattggtctt tattcaatgg agcttgttcc
     1621 taaagaagct gccggagccg cat
//
LOCUS       DAJVPT010000253         1633 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVPT010000253 DAJVPT010000000
VERSION     DAJVPT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..318)
                     /locus_tag="OQM47_004085"
     CDS             complement(<1..318)
                     /locus_tag="OQM47_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1482001.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIA"
     gene            complement(383..1555)
                     /gene="iadA"
                     /locus_tag="OQM47_004086"
     CDS             complement(383..1555)
                     /gene="iadA"
                     /locus_tag="OQM47_004086"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1482002.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
BASE COUNT          369 a          449 c          439 g          376 t
ORIGIN      
        1 ggctatcttg attttacgtt gcgcgtaatc gctgccgcca cgcagctctg caaggttgct
       61 ttctaactgt tgcaacagat ggcggatctg cgaatggaag attttccctt gttctgagag
      121 ttgcagtggc gtcacctggc ggttaaacaa ttcaacgcca atcgcctgtt ccagtgcacg
      181 gatgcggcgg ctgaatgccg gttgcgagac gttgcgactg actgccgcct gggaaaaatt
      241 gcggcatttt tccagagtca gaaaatcata aagccatttg gtttcaatat tatgcaaaac
      301 cgcaccacag tcatccatta cgttttactc tgtcagccgc ccttgggggc attctacgtc
      361 cattagggcg gctgacaacc gtttaagccg tttcaaacgt tcctttcacg caggctttgc
      421 cgtctttgac catcagtttg ccgcgagcgt atacctgctc aatgcgcaat tctggcgtca
      481 tgaccagtaa gtctgcgtca ttgcctggca gaatttcgcc tttcccgctc atgttaagga
      541 aaccggctac gctactggtg agcgggcgca gggcatcgct gatactgaaa tcatagtctt
      601 tgaccagcac ctgcacggtt tccagcaacg tttcaaaacc tgcaacaccg atatgggtta
      661 aattcccttc gtcatcgaag aacggctggc taccgttgcc gtcggagctg agggtgacgc
      721 gtgccagcgg aatacccgcc tgaacggcgc gggcaatacc ttcggcaggg gcgaccggtt
      781 cgtcaatgct gctggtgata tcgatggtgc cgcctttgcg cgcgaactcc agcgcctgct
      841 caaacaacgg tacgttgcgg ttaacgtggg tcggcagcag cttgctgatc ggcacatcgc
      901 agttttccag caggtcataa ataggcttta acgccttttt actgtcgccc atgtggaaca
      961 cggtgacgcc aggtttaccg ccgagcaaac cgccaacgcg ggattctgcc gccatattag
     1021 ccaggtgata aacgtccggt gcggcagaac ggtgatcaga gatggcgcat ttcacgccaa
     1081 tcacacgatc gataatcgcc acgtcttttt ccacggaacc cgtaatagtg cgggaaggga
     1141 catgataagc gccggtcagc atccaggcgc tgatgccttc ttcattgagc gcacgggtct
     1201 tggcgagcag ggattccggg tggcgagaga tagagtcggt gcccagcaga ccaaccactg
     1261 acgtgacgcc cgcttccgtc aggcgactta gcgccacttc cggcgtgcgc gtcgtgggac
     1321 ctgcttcgcc gccaccgcca atcaaatgga cgtgttgatc aataaaacct gggcagagga
     1381 tctgcccgct gagatcgaca accgtgcagt tcggtacaat gtcagaaggg atattgctgg
     1441 caacggcgat aattttgccg ttagcgacga ggacatcgca aattccccga tcttccggcg
     1501 catacaaatg cgctccctgc agcagggtaa aaccggctgc ggtataatca atcatggtaa
     1561 ctccttgtta aacaataagc tgcataaccc agatcgacag caatgcgtta atcacgcaga
     1621 ccgtaatgat atg
//
LOCUS       DAJVPT010000254         1600 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVPT010000254 DAJVPT010000000
VERSION     DAJVPT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..360
                     /locus_tag="OQM47_004087"
     CDS             <1..360
                     /locus_tag="OQM47_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1482003.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OQM47_004088"
     CDS             361..726
                     /locus_tag="OQM47_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1482004.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OQM47_004089"
     CDS             723..1406
                     /locus_tag="OQM47_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1482005.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWVWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          323 c          438 g          387 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggtgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJVPT010000255         1600 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVPT010000255 DAJVPT010000000
VERSION     DAJVPT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..1317
                     /gene="kup"
                     /locus_tag="OQM47_004090"
     CDS             <1..1317
                     /gene="kup"
                     /locus_tag="OQM47_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1482006.1"
                     /translation="LRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGG
                     EVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWAL
                     IPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNW
                     MLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILI
                     AFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEA
                     MIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYV
                     HNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESL
                     ILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1484..>1600
                     /locus_tag="OQM47_004091"
     CDS             1484..>1600
                     /locus_tag="OQM47_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="cytoplasmic mutarotase that catalyzes the
                     conversion between beta-pyran and beta-furan forms of
                     D-ribose; RbsD is required for efficient ribose
                     utilization in E. coli; rbsD-mutant E. coli strains are
                     unable to use ribose as a sole carbon source; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1482007.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTR"
BASE COUNT          337 a          389 c          427 g          447 t
ORIGIN      
        1 ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc tgaatccgat gtgggcggtg
       61 catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt ggtgctgtcg
      121 attacggggg gcgaggtgct gtatgctgat atggggcact ttggtaagtt ctctattcgc
      181 ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta attacttcgg tcagggagcg
      241 ttgttgttaa agaacccgga agcgattaag aacccgttct tcctgttggc accggactgg
      301 gcgctgatcc cactgctgat catcgccgca ctggcgacgg taattgcctc gcaggcggtt
      361 atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg
      421 cgcattattc acacctccga aatggagtca gggcaaatct atattccgtt tgtgaactgg
      481 atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag caacctggcg
      541 gcggcgtacg ggattgcggt gaccggaacc atggtactga cgtctattct ctcgactacc
      601 gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct gattgctttc
      661 ctttgtgtcg atattccatt gttcaccgct aacctcgata aactgctctc cggcggctgg
      721 ttgccattga gcctcggtac tgtgatgttt atcgtgatga ccacctggaa gagcgagcgt
      781 ttccgcttgc tgcggcggat gcatgaacat ggtaactctc tggaagcgat gattgcttcg
      841 ctggagaaat caccgcctgt tcgcgtgccc gggaccgcgg tgtatatgtc gcgtgcaatc
      901 aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt gcatgagcgg
      961 gtgattctgt taactctgcg caccgaagac gcgccatatg tccataacgt ccgtcgggta
     1021 cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg ttggcgagaa
     1081 acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag ttgccggatg
     1141 atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat
     1201 ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg tgcaccagat
     1261 caatttgaaa tcccgccaaa cagggttatc gaactgggta ctcaggtcga aatctaacgc
     1321 cagacgcctc ctttcttcat aagggggcgt ttttgttttc atggttaatc accatgtaaa
     1381 acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac tcagtcagga
     1441 ttaagctgcg gatcagcgaa acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt
     1501 tcttaattct gatatttcat cggtgatctc ccgtctggga cataccgata cgctggtggt
     1561 gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt
//
LOCUS       DAJVPT010000256         1598 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVPT010000256 DAJVPT010000000
VERSION     DAJVPT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            658..1257
                     /locus_tag="OQM47_004092"
     CDS             658..1257
                     /locus_tag="OQM47_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1482008.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          414 a          380 c          368 g          436 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tatatggctt cacccgtgtt aatatcctgg
       61 ataactgtgt ttgaaggatt tctgttaata atctgctcct gcaactcacc tgatgagtat
      121 tagtggtaat acataatctt actcgtaaaa gagtatacct tatgtcatta aacagtcttc
      181 cggcgtgttg ttcaatcctg tacctctgtt acttatctgg tacgttgata tgtgtaatgc
      241 tacgttaatt gcaatatctg gttatggctt tataccttca gcatttaaaa tattttatta
      301 ccggagaaac agggaagtgg aaagactttt gggataatgt caggccattc tgacgttaat
      361 cacagtacca aataacgaga gggtaatgga agttaaaacc tgcgtaagtt cctctgttgt
      421 tcactggaaa aagtgattgc ccacacgcgt tataaaggcc tttccgctgc agcctggtta
      481 acagagcttc aggcgttata tgcgctgtat ggctgttacg tatgctgttc tttgcctggt
      541 tctgataccc gtggaagtcg ttaccggagg aaacacgttc accctactac ctgagaacat
      601 cttcttcaaa aattacggca acgcttcttc ctgtgttaca gaacacgagg aagaaacatg
      661 caaacttata acaccaatcc cgcttacgaa atggctcccc agctcctgga gcactttaac
      721 cagcatctgg attcattatt cggggtctat tcgaaactgt taccgttcag aatggatttt
      781 gcttaccgaa aaaacaccct cagctacaga tgtgcctgca ggtacgccat gtgcgcggag
      841 atgctgcaac tgattaacga ggtgggagag aaactggtcg ggtatgcctg ggtgatggaa
      901 tatacagaac gtaaagggct tcacattcat tttgtgggct atctgaacgg ccagagccac
      961 aggagttcgt atctggtatc cagattgatg ggagatatct ggaggcgggt taccgagggt
     1021 aatggttact accactggtg ccggtttaat aaaaattatc cggtaaacat taaccacgtc
     1081 attcactatt cagatcacaa agcggtgaat gatttacgct atgccatcag ctacctggcc
     1141 aaacgggaac agaaagaatg tggcatcatc ctgaaatgca gcgggcttcc tgaaaaaagt
     1201 aaccggggtc gaccacgact ggacagcccc ctgccgggga tatgtgctct ggtgtaaacc
     1261 cctgaacgac aagtacgggc tggcgctaat ctttgctgtc ggttccggcg gccacttcag
     1321 cggcactgcg cattttttac ggcgtggtca ttccgtatga accatacgga gatttaacca
     1381 tgacacattc tttaccattc cttccggcaa ccgatacagc acatccccca atctccatct
     1441 ctgcttactg actgtttttt acgtcagtgg gccattgtgt gacaaccaca gtgctgtaac
     1501 agcaaccagc ctgctttacc caaactccag tccagatttt aactgaaccg ccattcacac
     1561 cctgaccacg ctgcccgtac cgggacggct gttgcctg
//
LOCUS       DAJVPT010000257         1598 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVPT010000257 DAJVPT010000000
VERSION     DAJVPT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..405
                     /locus_tag="OQM47_004093"
     CDS             <1..405
                     /locus_tag="OQM47_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1482009.1"
                     /translation="EVSLQPYGVFTHRHQPEIATHLGADVIFTPHLGNFPRGILETIT
                     CRLKSGVTQAQVAQALQQAYAHKPLVRLYDKGVPALKNVVGLPFCDIGFAVQGEHLII
                     VATEDNLLKGAAAQAVQCANIRFGYAETQSLI"
     gene            413..1189
                     /gene="argB"
                     /locus_tag="OQM47_004094"
     CDS             413..1189
                     /gene="argB"
                     /locus_tag="OQM47_004094"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1482010.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            1250..>1598
                     /locus_tag="OQM47_004095"
     CDS             1250..>1598
                     /locus_tag="OQM47_004095"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1482011.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATD"
BASE COUNT          381 a          386 c          483 g          348 t
ORIGIN      
        1 gaagttagcc tgcaaccgta tggcgtcttt actcatcgcc atcaaccaga gatcgccaca
       61 cacctcggtg ctgacgttat cttcacccca catctgggca atttcccgcg cggcattctc
      121 gaaaccatta cctgccgcct gaaatcgggt gtgacccagg cgcaagtcgc gcaagcgtta
      181 cagcaggcgt atgcccataa accgctggtg cggctgtatg acaaaggcgt tccggcgctg
      241 aaaaatgtcg ttgggctgcc attttgcgat atcgggtttg ccgttcaggg cgagcatctg
      301 attattgtgg cgaccgaaga caacttactg aaaggcgcgg cggcacaagc ggtacagtgc
      361 gccaatattc gtttcggcta tgcggaaacg cagtctctta tttaagggtg caatgatgaa
      421 tccattaatt atcaaactgg gcggcgtact gctggatagt gaagaggcgc tggaacgtct
      481 gtttagcgca ttggtgaatt atcgtgagtc acatcagcgt ccgctggtga ttgtgcacgg
      541 cggcggttgc gtggtggatg agctgatgaa agggctgaat ctgccggtga aaaagaaaaa
      601 cggcctgcgg gtgacgcctg ctgatcagat agacattatc accggagcac tggcgggaac
      661 ggcaaataaa accctgttgg catgggcgaa gaaacatcag attgcggccg taggtttgtt
      721 tctcggtgac ggcgacagcg tcaaagtgac ccagcttgat gaagagttag gtcatgttgg
      781 actggcgcag ccaggttcgc ctaagcttat caactccttg ctggagaacg gttatctgcc
      841 ggtggtcagc tccattggcg taacagacga agggcaactg atgaacgtca atgccgacca
      901 ggcggcaacg gcgctggcgg caacgctggg cgcggatctg attttgctct ccgacgtcag
      961 cggcattctc gacggcaaag ggcaacgcat tgccgaaatg accgccgcga aagcagaaca
     1021 actgattgag cagggcatta ttactgacgg catgatagtg aaagtgaacg cggcgctgga
     1081 tgcggcccgc acgctgggcc gtccggtaga tatcgcttct tggcgttatg cggagcagct
     1141 tccggcactg tttaacggta tgccgatggg tacgcggatt ttagcttaag ttttgttagc
     1201 cggaggcgca gctttccggc attgaatttc aaaataagga aacagagtta tggcactttg
     1261 gggcgggcgt tttacccagg cagcagatca acggttcaaa caattcaacg actcactgcg
     1321 ctttgattac cgtctggcgg agcaggatat tgttggctct gtggcctggt ccaaagcctt
     1381 ggtaacggtc ggcgtgttaa ccgcagaaga gcaggcgcaa ctggaagagg cgctgaacgt
     1441 gttgctggaa gatgttcgcg ccaggccaca acaaatcctt gaaagcgacg ccgaagatat
     1501 ccatagctgg gtggaaggca aactgatcga caaagtgggc cagttaggca aaaagctgca
     1561 taccgggcgt agccgtaatg atcaggtagc gactgacc
//
LOCUS       DAJVPT010000258         1573 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVPT010000258 DAJVPT010000000
VERSION     DAJVPT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..318
                     /gene="tnpB"
                     /locus_tag="OQM47_004096"
     CDS             <1..318
                     /gene="tnpB"
                     /locus_tag="OQM47_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482012.1"
                     /translation="WLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIFIFRGRSGSQV
                     KLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSL
                     TML"
     gene            368..>1573
                     /locus_tag="OQM47_004097"
     CDS             368..>1573
                     /locus_tag="OQM47_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HCS1482013.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLF"
BASE COUNT          346 a          442 c          478 g          307 t
ORIGIN      
        1 tggctggttg ccggtatcac cgatatgaga aatagcttca acggcctggc tgcgaaagta
       61 caaacggcgc tgaaagacga tcccatgtcc ggccatattt tcattttccg gggccgcagc
      121 ggcagtcagg ttaaactgct gtggtccacc ggcgacggac tgtgcctcct gaccaaacgg
      181 ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag
      241 gcgcagctgg cgatgctgct ggaaggtatc gactggcgac agcccaagcg gctgctgacc
      301 tccctgacca tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata
      361 cgggcttatg aacgacatct cttctgacga catcttcctg ctgaaacagc gcctggccga
      421 acaggaagcg ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga
      481 ccatctgcag gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa
      541 agtctcccgc cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga
      601 tacgctgact ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac
      661 ccgtaagccg ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcggcgcc
      721 gtgctgcccg aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct
      781 ggagttgatg cgtagcgcct tccgggttat ccggacggta cgggaaaaac atgcctgtac
      841 tcagtgcgat accatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc
      901 cggaccgggg ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta
      961 ttgccagtca gaaatatacg gccggcaagg tgtggagctg agccgttcac tgctgtcggg
     1021 ctgggtggat gcatgctgcc ggctgctgtc tccgctggaa gaggcgcttc atggctatgt
     1081 catgactgac ggcaaactcc atgccgatga taccccggtc caggtactgc tgccgggtaa
     1141 taagaagacg aagaccgggc ggttgtgggc gtatgttcgt gatgaccgca atgccgggtc
     1201 agcgttggca cctgcagtgt ggttcgctta cagcccggac agaaaaagca tccatccgca
     1261 gactcatctt gcttgcttca gcggtgtgct gcaagcggat gcgtacgccg ggttcaacga
     1321 gctgtatcgc aatggtggga taacggaagc tgcctgctgg gctcatgccc gccgaaagat
     1381 ccacgatgtg cacgtccgca tcccgtcagc actgacggaa gaagccctgg agcagatcgg
     1441 tcagttgtac gccatagagg cggatataag gggaatgccg gcagagcagc ggcttgctga
     1501 acgtcagcga aaaacgaaac cgctgttgaa atccctggaa agctggttgc gtgaaaagat
     1561 gaagaccctg ttc
//
LOCUS       DAJVPT010000259         1503 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVPT010000259 DAJVPT010000000
VERSION     DAJVPT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            240..509
                     /locus_tag="OQM47_004098"
                     /pseudo
     CDS             240..509
                     /locus_tag="OQM47_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OQM47_004099"
                     /pseudo
     CDS             506..732
                     /locus_tag="OQM47_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OQM47_004100"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OQM47_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCS1482014.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          443 a          300 c          357 g          403 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 cca
//
LOCUS       DAJVPT010000260         1501 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVPT010000260 DAJVPT010000000
VERSION     DAJVPT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..487
                     /locus_tag="OQM47_004101"
     CDS             <1..487
                     /locus_tag="OQM47_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1482015.1"
                     /translation="AESSIKTTTNPPAFGCTFVDLTVDIALCKVTINRILNVHDSGHI
                     LNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGVVRNPNLLDYKMPTMPDLPQLESAF
                     VEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMATGVAINTLPLTPKRLYEEFHLAGL
                     I"
     gene            498..1376
                     /gene="xdhB"
                     /locus_tag="OQM47_004102"
     CDS             498..1376
                     /gene="xdhB"
                     /locus_tag="OQM47_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1482016.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            1373..>1501
                     /locus_tag="OQM47_004103"
     CDS             1373..>1501
                     /locus_tag="OQM47_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="iron-sulfur-binding subunit; with XdhA and XdhB
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase"
                     /protein_id="HCS1482017.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQG"
BASE COUNT          375 a          421 c          367 g          338 t
ORIGIN      
        1 tgctgaaagc tccatcaaaa ccaccactaa cccaccggcg tttggctgta cctttgttga
       61 tctgacggtc gatattgcgc tgtgcaaagt caccatcaac cgcatcctca acgttcatga
      121 ttcagggcat attcttaatc cactgctggc agaaggtcag gtacacggcg gaatgggaat
      181 gggcattggc tgggcgctat ttgaagagat gatcatcgat gctaaaagcg gcgtggtccg
      241 taaccccaat ctgctggatt acaaaatgcc gaccatgccg gatctgccac aactggaaag
      301 cgcgttcgtc gaaatcaatg agccgcaatc cgcatacgga cataagtcac tgggtgagcc
      361 acccataatt cctgttgccg ctgctattcg taacgcggtg aagatggcta ccggtgttgc
      421 aatcaataca ctgccgctga cgccaaaacg gttatatgaa gagttccatc tggcaggatt
      481 gatttgagga taacatcatg tttgattttg cttcttacca tcgcgcagca acccttgccg
      541 atgccatcaa cctgctggct gacaacccgc aggccaaact gctcgccggt ggcactgacg
      601 tactgattca gctccaccat cacaatgacc gttatcgcca tattgttgat attcataatc
      661 tggcggagct gcggggaatt acgctggcgg aagatggctc gctacgtatc ggctctgcaa
      721 cgacatttac ccagctaata gaagatccta taactcaacg tcatctcccg gcgttatgtg
      781 ctgcggccac gttcattgct ggaccgcaga tccgtaacgt cgctacctac ggtggaaata
      841 tttgcaacgg tgccaccagc gcagattctg ccacgccaac gctaatttat gacgcgaaac
      901 tggagatcca ctccccgcgc ggtgttcgtt tcgtcccgat taatggcttt cacaccgggc
      961 cgggcaaagt gtctcttgag catgacgaaa tcctcgtcgc ctttcatttt ccgccacagc
     1021 cgaaagaaca cgcgggcagc gcgcatttta aatatgccat gcgcgacgca atggatattt
     1081 caacgattgg ctgcgccgca cattgccgac tggataacgg caatttcagc gaattacgcc
     1141 tggcatttgg tgttgccgcg ccaacgccga ttcgctgcca acatgccgaa cagactgcac
     1201 aaaatgcgcc attaaacctg caaacgctgg aagctatcag cgaatctgtc ctgcaagatg
     1261 tcgccccgcg ttcttcatgg cgggccagta aagagtttcg tctgcatctc atccagacga
     1321 tgaccaaaaa agtgattagc gaagccgtcg ccgcggcggg gggaaaattg caatgaatca
     1381 cagcgaaaca attaccaccg aatgcaccat taacgggatg ccttttcagc ttcacgccgc
     1441 accaggcacg ccgctctcgg aattactccg cgaacaagga ctgctaagtg tcaaacaagg
     1501 g
//
LOCUS       DAJVPT010000261         1444 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVPT010000261 DAJVPT010000000
VERSION     DAJVPT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..275)
                     /locus_tag="OQM47_004104"
                     /pseudo
     CDS             complement(1..275)
                     /locus_tag="OQM47_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(390..1028)
                     /gene="ospI"
                     /locus_tag="OQM47_004105"
     CDS             complement(390..1028)
                     /gene="ospI"
                     /locus_tag="OQM47_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1482018.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1187..1444)
                     /locus_tag="OQM47_004106"
                     /pseudo
     CDS             complement(1187..1444)
                     /locus_tag="OQM47_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          413 a          359 c          248 g          424 t
ORIGIN      
        1 gccagtctac ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct
       61 gaggcaggaa cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat
      121 tttttccact taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc
      181 gtcatgccag gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt
      241 ttacgaagct ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac
      301 atatatccaa gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc
      361 ggctcaaatc tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta
      421 ctgttagcat agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt
      481 tcatctacaa atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct
      541 gtttgaacat ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg
      601 gcctgttcaa tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct
      661 gcaatacgct cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta
      721 tcaatttgct ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt
      781 acgggagcct ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct
      841 gaacagttac catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca
      901 gaatctttat ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca
      961 tttacatttc cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca
     1021 ttaatcatat tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc
     1081 cctctgatat gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc
     1141 gtgtctaaaa agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca
     1201 ggactaacag ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac
     1261 cgccagtttt agtgttgtac gaataccaga gccagtgttg ttgcagcctt gctgccaacg
     1321 aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca
     1381 gatgttatcc gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt
     1441 tcag
//
LOCUS       DAJVPT010000262         1441 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVPT010000262 DAJVPT010000000
VERSION     DAJVPT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OQM47_004107"
     CDS             complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OQM47_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1482019.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPD"
     gene            complement(1251..>1441)
                     /locus_tag="OQM47_004108"
     CDS             complement(1251..>1441)
                     /locus_tag="OQM47_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1482020.1"
                     /translation="ENEYSQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLT
                     DEVLALRLSENGSRLHHS"
BASE COUNT          392 a          289 c          316 g          444 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac aatggattcc
       61 ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt aatgaagtta
      121 gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat aatctgcgta
      181 gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt agttcctgta
      241 gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc aagacagtaa
      301 ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt aacgtctcca
      361 gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc agacatgaca
      421 aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc gggagtatat
      481 tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca ggtagggaga
      541 ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt ggtaaagaac
      601 gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat attttcaata
      661 atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag tcatcccaga
      721 tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata catgataagc
      781 cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa cccctcacta
      841 ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa acaatcctag
      901 agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc cagtaaccgt
      961 cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat ccagtttgga
     1021 ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt actgtctcga
     1081 tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt gcacgccgta
     1141 aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt aatcgggtca
     1201 cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga ttatgaatgg
     1261 tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt caactgacgg
     1321 taaatctgct gttcagtctc acgcatcacc tgtgcaccgg cttccctctc cgcatccgca
     1381 tcaccgctca gacctgatgc tttcagccgg tcagccaccc tctgagagta ctcattctcc
     1441 a
//
LOCUS       DAJVPT010000263         1437 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVPT010000263 DAJVPT010000000
VERSION     DAJVPT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..757)
                     /gene="ipaH7.8"
                     /locus_tag="OQM47_004109"
     CDS             complement(<1..757)
                     /gene="ipaH7.8"
                     /locus_tag="OQM47_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1482021.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPD"
     gene            1086..1265
                     /locus_tag="OQM47_004110"
                     /pseudo
     CDS             1086..1265
                     /locus_tag="OQM47_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1282..1437)
                     /locus_tag="OQM47_004111"
                     /pseudo
     CDS             complement(1282..1437)
                     /locus_tag="OQM47_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          450 a          219 c          310 g          458 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac aatggattcc
       61 ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga agagtggtta
      121 tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca gaaaattgag
      181 gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc ggtaagtcgg
      241 gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg ggaagagccg
      301 gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt ggcaaaacag
      361 gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca ggaagttctg
      421 gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca ggcagctttg
      481 gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt ggtaattcgg
      541 gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc tgaaagcact
      601 gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc ttttcccatt
      661 cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata gagggggagc
      721 aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa ccttatattt
      781 gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat ttccgatatt
      841 aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta tttatggagg
      901 ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg aattcaacat
      961 tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca tattttagtc
     1021 aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa aacgatacac
     1081 atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag aaatgtgtgc
     1141 cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg tcaaaccatg
     1201 ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg aatgagtttt
     1261 gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac cgatgacttt
     1321 ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg
     1381 ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc ggtcagg
//
LOCUS       DAJVPT010000264         1433 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVPT010000264 DAJVPT010000000
VERSION     DAJVPT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            82..1275
                     /locus_tag="OQM47_004112"
     CDS             82..1275
                     /locus_tag="OQM47_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCS1482022.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1433)
                     /locus_tag="OQM47_004113"
                     /pseudo
     CDS             complement(1295..1433)
                     /locus_tag="OQM47_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          421 a          309 c          336 g          367 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aac
//
LOCUS       DAJVPT010000265         1418 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVPT010000265 DAJVPT010000000
VERSION     DAJVPT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..496)
                     /locus_tag="OQM47_004114"
     CDS             complement(<1..496)
                     /locus_tag="OQM47_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482023.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQ"
     gene            704..>1418
                     /gene="ldhA"
                     /locus_tag="OQM47_004115"
     CDS             704..>1418
                     /gene="ldhA"
                     /locus_tag="OQM47_004115"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1482024.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALI"
BASE COUNT          368 a          322 c          362 g          366 t
ORIGIN      
        1 cttgaaattt aactttttcg ccctgataac gcagttgctg gatatcagag gttaatgcga
       61 gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg atattgatcc
      121 aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct ggcgattgct
      181 ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccgtgccg acgttcaata
      241 accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga caatctccca
      301 ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg ctttcatcta
      361 atgcaatacg tgtcccgagc ggtagccaga tgtccgccag cgtgggaacc cacagcccga
      421 gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg agaacggctt
      481 tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa gcattcaata
      541 cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta agcatagttg
      601 ttgatgaatt tttcaatatc gccatggctt tcaattatat ttgaaatttt gtaaaatatt
      661 tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac tcgccgttta
      721 tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct ttggctttga
      781 gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca atggctgcga
      841 agcggtatgt attttcgtaa acgatgacgg cagccacccg gtgctggaag agctgaaaaa
      901 gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg accttgatgc
      961 ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag aggccgttgc
     1021 tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg
     1081 tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt atggcaaaac
     1141 ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt
     1201 tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg aactcggtgt
     1261 ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc tgcactgccc
     1321 gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg
     1381 cgtgatgatc gtcaatacca gtcgcggtgc attgattg
//
LOCUS       DAJVPT010000266         1406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVPT010000266 DAJVPT010000000
VERSION     DAJVPT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..665)
                     /locus_tag="OQM47_004116"
     CDS             complement(<1..665)
                     /locus_tag="OQM47_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS1482025.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
     gene            complement(616..1380)
                     /locus_tag="OQM47_004117"
     CDS             complement(616..1380)
                     /locus_tag="OQM47_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482026.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
BASE COUNT          329 a          373 c          318 g          386 t
ORIGIN      
        1 gccgcatcat catcagactc cggaacgccc tcatacacat gtttaaaacc atccggatca
       61 cggcgtcgcg ccgcgtcgat aaccttaagc atggtgtcag acagaaacgg attttcgtca
      121 tagttaattt tgcggatgag agtgccttcg ggcggatcaa caacgaagtt gcgccagacg
      181 aaatcagtaa caagtccggg gttgaatatg aaccagcatt ccgaaccctc tttacggatc
      241 gttggctcca gaattttcca ctggtattcc gtcagtgcgt gggcttcttc cagccacaac
      301 atatcgatcc cctccagtga cttaatttct tcgatgttgc gccataatcc ataaaaaaca
      361 aattccgagc cagtaacccg gttaatgatt ttgttgttca gaatacggaa acggtgccgc
      421 agaccaaacc tgtctatctg aattttgagc agggtataca ccgactcttc gattttattc
      481 tggatctgac gggcacaaca aaaacgcagg gtgtatttat tcgacagaaa tatggcaatg
      541 ccagcggcat cccatgattt tgacgatgac cgaccaccat aaagcacttt gttacgtgcc
      601 cgcatagtcc agaaactacg taaagccgga ttcagcgtcg gtttggatgt cagagtagaa
      661 gtcattgagg tcacgctctc cattgccatc atcaatacct gcatcacggc gaagacgatc
      721 agcctccaga gacaccttat cagtggcggc cttgcggtag gctgtatcag caaatatttt
      781 tcctaccgtc gcaagcgtgc caacgatgga ctcaatacga actgtattgc gcatcattgc
      841 tttctcggca gcgctgatat tttcaatcag catcttcctt tcctggtccc cattagcatc
      901 ttccagcgac accaaccacc gaccaatatt ctctgcagcg acaaggttgt tagcacgaag
      961 gcgaaataat tcgtcctcga gcgtcaacgc tttagcgtcc tctatcacct catctttgag
     1021 cagaaggcga cgggcataac caccgtgttt taatgcctgc tggttaccgg gttggaatgg
     1081 gttagtcggg ggatcggtac gcattccgcg tatcggtttc gtatccggtg tagattctgt
     1141 ttttagttgc gtactttttt gtgtaaggcc agtaatggca ggctttctgc tggtacgcac
     1201 tttccttttt tgcgtaccat ttttgcaaac ctgcgtaccg ccactgcgtg cccaatcaag
     1261 ctttttggcc ctcttcctga tagccccttc tgtaacgccg tatttctcgc ctatatcacg
     1321 gaggctaagg actccggccc ggtatgccga ttcgatggcc tcccagtccg gttttgccat
     1381 gattatgctt cctgtgattt actgtt
//
LOCUS       DAJVPT010000267         1399 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVPT010000267 DAJVPT010000000
VERSION     DAJVPT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..303)
                     /locus_tag="OQM47_004118"
     CDS             complement(<1..303)
                     /locus_tag="OQM47_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCS1482027.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFSACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALAR"
     gene            complement(296..868)
                     /gene="kdpC"
                     /locus_tag="OQM47_004119"
     CDS             complement(296..868)
                     /gene="kdpC"
                     /locus_tag="OQM47_004119"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1482028.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(877..>1399)
                     /locus_tag="OQM47_004120"
     CDS             complement(877..>1399)
                     /locus_tag="OQM47_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482029.1"
                     /translation="GRLVAMTGDSTNDATALAQADVAVAMNSGTQAAKEAGNMVDLDS
                     NPTKLIEVVHIGKQMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLH
                     SPDSAILSAVIFNALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGI
                     KVIDLLLTVCSLV"
BASE COUNT          316 a          401 c          380 g          302 t
ORIGIN      
        1 gcgggcgagg gcggcatcga gatcaaactc gctgatatgc cgcccacggt acgcctggcg
       61 tttcagaggc agaacagcca gcccttcaag catggcggcg gtatctttcc gcccatgggt
      121 ttctaccaca ccaaccacaa tatccagccc ttgcgcccgc agtcgctggg cttctgccag
      181 catcgcccag gtcttcccga cgcctgcaca ggcactgaag aaaactttca gcttcccccg
      241 atgcggcgcg gcagtttgtt ccagcagacg gtcggggtcg ggacgtaagg gttcgttatt
      301 catcaagctt atccagtgcc agattgagtt cgacaatgtt gacgaccggc tggccgatat
      361 atttcaccag cggttgttgg ctgtattttg cgatcagctg cgtgagctgt tcaacgctga
      421 gattacgcgc ttttgcgatg cgtgggatct gccaggccgc cgcttgcggg gtgatgttat
      481 tgtccagtcc gcttgccgac gccgtcacca gttcaaccgg aacgttcgtg ctggcgtccg
      541 ggttagcggc ccgtaatgcg gcaacgcgtg cggctatctg tttatccagc tccgggttac
      601 tgactgccag attgctcccg ccagaagcct gtggattata gggcatttct gctgttgccg
      661 acgggcgacc atgaaaatag ccgttgccgg taaaattttg cccgattaat gccgaaccgc
      721 gcaccgtatc accttcacga atcaacgaac cgttggcctg ccagggatac caccattgcc
      781 ccagtgcggt ggtcagcagc gggtaaacgc cgccagtaat caataacaga aagagaaatg
      841 tcgataatgc cggacgtaat ccactcatgg taaacctcac accagactac aaacggttag
      901 cagtaaatca atgactttga taccaataaa cggcaccagc agcccaccca gaccgtaaat
      961 ccataagtta cggcgcaaca tggcagaagc ggtaagcggt ttataactca cgccttttaa
     1021 cgccagagga atcaaaaaga cgataatcaa ggcgttgaaa atgaccgcac tgagaatggc
     1081 ggagtcgggc gaatgcaggc gcatgatgtt cagcgcattt aactgcggat atgttgccgc
     1141 gaacgccgcc ggaataatgg cgaagtattt cgccacatcg ttggcaatgc tgaaggtggt
     1201 cagcgagcca cgggtcatca gcatctgttt gccaatgtgc accacctcga tcaacttggt
     1261 cgggttagag tcgagatcca ccatattgcc cgcctcttta gccgcctggg tgccggagtt
     1321 catcgccacc gcgacatctg cctgcgccag cgccgtagca tcgttggtgc tgtcgccggt
     1381 catcgctacc aaacgacct
//
LOCUS       DAJVPT010000268         1385 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVPT010000268 DAJVPT010000000
VERSION     DAJVPT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..185
                     /locus_tag="OQM47_004121"
     CDS             <1..185
                     /locus_tag="OQM47_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482030.1"
                     /translation="KIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIKNNGK
                     KIRSDKKHFLECNEDK"
     gene            complement(984..1320)
                     /locus_tag="OQM47_004122"
                     /pseudo
     CDS             complement(984..1320)
                     /locus_tag="OQM47_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          409 a          308 c          296 g          372 t
ORIGIN      
        1 acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt gccactaaat
       61 ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa gacctcatta
      121 aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc aatgaagaca
      181 aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat cagcgcactt
      241 gattttcttt atattcactg acgagcttgc cggggcacct ttgtttgccg gtcattctgg
      301 tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat aaggcctgag
      361 cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat atggtgcatg
      421 taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg acaaagtggt
      481 gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac aaaggccaac
      541 gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt acctgtcgcc
      601 agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa aggtcggtga
      661 tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca gaggatgccc
      721 cgtcgctgca tatggatcag ggttggcaat atcgaatcgc aggctatagg caaagttaaa
      781 gccccatgga gtagcacaaa atatgccgca caaaggaaac ggtctgaata acgcagtgat
      841 gaagaacttc ttcagcacac tgctaaaacg catagtgatc gagcgctgat tctggcgaac
      901 aactgaacta atacatcaga atctgcatta tgttaaataa atatgaaaag atggtttaaa
      961 taccccgtta cttttgactt acactatacg gtatcgcatc gtttaatatt cgcaccggcc
     1021 agatttttat ttctattagt tgtcacaata ctgaatgcgt acgaccacag tattctggct
     1081 cctgtgtggt tatgctttaa ttctgcgttc cgggcagata agcagttgct tgcaggaatc
     1141 cttcttgtgt taatgtcagt tccccttttt accagtgctg atttccacat tccgtccaac
     1201 agagcttata gcctttccct ggattatagc attgtccggc tgaagttctt tttgaataat
     1261 aatagaagca ctgctggcag atccagtccg tttttcataa cccactgtac tgataaccat
     1321 aatctaatca gtagaaattg agtcgaaaat aagcactact ctatacagga taattagagg
     1381 tcagt
//
LOCUS       DAJVPT010000269         1384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVPT010000269 DAJVPT010000000
VERSION     DAJVPT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..157
                     /locus_tag="OQM47_004123"
     CDS             <1..157
                     /locus_tag="OQM47_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1482031.1"
                     /translation="STPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKER
                     RKEWGTL"
     gene            142..780
                     /locus_tag="OQM47_004124"
     CDS             142..780
                     /locus_tag="OQM47_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCS1482032.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1008..>1384
                     /locus_tag="OQM47_004125"
     CDS             1008..>1384
                     /locus_tag="OQM47_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482033.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLI"
BASE COUNT          405 a          252 c          344 g          383 t
ORIGIN      
        1 cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga atgatttctg
       61 gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt acctgcagcg
      121 tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact tggcaacatg
      181 attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc tctgaatcat
      241 ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga ctgtgtgttg
      301 tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat ggcactaggt
      361 gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc ggtggtggtg
      421 tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt actggggcgg
      481 gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat taatccggag
      541 agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc cagggggaaa
      601 caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg ctggacgaat
      661 gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct gaaacagatt
      721 agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac ggttcgttga
      781 tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa aaatgatacc
      841 gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta ggctttgata
      901 acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg cagatttaca
      961 aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg aaaaaacaga
     1021 tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg aaaatattta
     1081 aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc gaaccattcc
     1141 acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata tttattaata
     1201 aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa agaagagata
     1261 tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta tttgatcatg
     1321 acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat tatgttttga
     1381 ttga
//
LOCUS       DAJVPT010000270         1356 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVPT010000270 DAJVPT010000000
VERSION     DAJVPT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            594..878
                     /locus_tag="OQM47_004126"
     CDS             594..878
                     /locus_tag="OQM47_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCS1482034.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            878..1153
                     /locus_tag="OQM47_004127"
     CDS             878..1153
                     /locus_tag="OQM47_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1482035.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          362 a          341 c          340 g          313 t
ORIGIN      
        1 cgacaacaag tcccattccc agtgtcgcaa caattgagcc gaagatatag gaaaatgtgc
       61 ctgacagccc taacagaata agtttacgcg taaatcgccc tgaatccaga agagcattaa
      121 gtatcacata aacaggttcc ataccagtcc tgaccgcatc aggacgccag taaataaaat
      181 ctcgtagtcc accggcatca gagacaagag taatcgtcgt taccggtatt tcgactggtt
      241 tgtactggtg tgagcggaca aaactactgt acgtctctca tacacgctcg taagcggccc
      301 tcacggcccg ctaacgcgga gatacgcccc gactgcgggt aaacccttgt cgggaccact
      361 ccgaccgcgc acagaggcat tttcatcgct gacaggccgc atggcctcgc agagccggag
      421 ggggactggt gatattcgga cgaccgtacc ggcttgcgcc gggcttcggc ggttttacac
      481 ctgtgctaca tgtaaccacc gtatgccgcc ttatatgcgc tgactcgcat tatttgcctt
      541 gttccgtctg aaagtaatca ctatgattaa atatgattaa caactaatcg ggtatgcaaa
      601 tgaaaaacaa taccgcacaa gcaacaaaag taattaccgc gcatgtgcca ttacctatgg
      661 ctgataaagt cgaccagatg gccgccagac tggaacgctc ccggggctgg gttatcaaac
      721 aggcgctttc tgcatggctt gcccaagagg aggagcgtaa tcgcctgacg ctggaagccc
      781 tggacgatgt gacatccgga caggttatcg accatcaggc tgtacagtcc tgggcggaca
      841 gcctcagtac tgaccatctg ttaccggtgc cacgctgatg gaactgaagt ggaccagtaa
      901 ggcactttct gatttggcgc ggttatatga ttttctggtg ctggccagta aacctgctgc
      961 cgccagaacg gtgcagtccc tgacacaggc gccggtcatt ctgttaactc atccacgtat
     1021 gggagaacag ttgtttcagt ttgaacccag ggaggtcaga cggattttta ctggcgagta
     1081 cgaaatccgt tacgaactta ctggccagac tatttatgta ttgcgcctgt ggcacacacg
     1141 agagaacagg tagattgggt gtggaggcaa ctttaacata gcctgcaggt attaattctg
     1201 gagcattgtt atcaggccag atattttgca ttccattaaa taatcttgta ggagataaaa
     1261 taaattacga tgaaataacg aaaattaccg ctcgtgactg gcggcaatat cgcgctccgg
     1321 gctggcaaat aacccatcag aaacgttatt gccaga
//
LOCUS       DAJVPT010000271         1351 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVPT010000271 DAJVPT010000000
VERSION     DAJVPT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            316..>1351
                     /locus_tag="OQM47_004128"
     CDS             316..>1351
                     /locus_tag="OQM47_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1482036.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEEN"
BASE COUNT          444 a          295 c          214 g          398 t
ORIGIN      
        1 atgtatataa gcttatttta tacatgatta ttagcaacaa atattaccaa gtcaagtagt
       61 caggctggct aaaatagttg gagatgttgt agtatcttac tcaagactaa tgtcttctgg
      121 tataaatcct ctgcattgtg aatatgtaaa acatcaaaaa aagatgaacc aaatatacga
      181 aaaaagcaaa tatacttacc agggtagaaa gcatgatata acactcattc ccttatggca
      241 aatattctga aacaccctcc tcattaggag agatacatta aatacaattt attcacaatc
      301 taattccata tccatatgcg agaaattaat atgctcagaa acatttcatc ctgtttattt
      361 ccacatatca gcacaattac atcccccaac cattatttgt ccgaatggga tgattgggag
      421 aaacaggggt taccggaaga acagcgtact gaggcggtaa gaagacttcg tgcatgtctt
      481 acctctaagg ggcataaact ggacctgcga gccttggcgc tttcctcgct acctgtactc
      541 cctgcttgca ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta
      601 cctgaaaata ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta
      661 ccacattatc taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta
      721 ccagacacca tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta
      781 cccttgaatt taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg
      841 ccttctaatc ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta
      901 cctaggagac tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc
      961 caagaaaata ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc
     1021 ccgacaaatc tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta
     1081 tcagatcaaa ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct
     1141 cagtttcgta tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa
     1201 aatttacatt cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc
     1261 cctcttcccg aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca
     1321 caatggactt ctttttccac cgaggagaat t
//
LOCUS       DAJVPT010000272         1344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVPT010000272 DAJVPT010000000
VERSION     DAJVPT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..473)
                     /locus_tag="OQM47_004129"
     CDS             complement(<1..473)
                     /locus_tag="OQM47_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1482037.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLF"
     gene            complement(464..766)
                     /locus_tag="OQM47_004130"
     CDS             complement(464..766)
                     /locus_tag="OQM47_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1482038.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1038..1310
                     /locus_tag="OQM47_004131"
     CDS             1038..1310
                     /locus_tag="OQM47_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482039.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          394 a          339 c          287 g          324 t
ORIGIN      
        1 taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc ataaaatgct
       61 ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg atcagccaga
      121 ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg cccctgggta
      181 caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg catgttatgc
      241 cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga gtagtatccc
      301 acaatacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt gagcatctgg
      361 tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca gtcaaatggc
      421 tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc cattcaggct
      481 ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc agttgcgtaa
      541 taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg ataatgtttt
      601 tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg accaagctcg
      661 cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc tgatgttctc
      721 gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt aacaatttgc
      781 tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac ttacgggaaa
      841 agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg atctgtgtcg
      901 gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga aatgcctgaa
      961 ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt cagagtaacc
     1021 atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac accagaatgg
     1081 tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag tccgtatttg
     1141 cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc ttccacaaaa
     1201 tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt cgttttgatt
     1261 tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga ttttcagccc
     1321 cgaccgctga aacgcctgtt caca
//
LOCUS       DAJVPT010000273         1339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVPT010000273 DAJVPT010000000
VERSION     DAJVPT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1169)
                     /gene="treF"
                     /locus_tag="OQM47_004132"
     CDS             complement(<1..1169)
                     /gene="treF"
                     /locus_tag="OQM47_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCS1482040.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAV"
BASE COUNT          318 a          342 c          358 g          321 t
ORIGIN      
        1 tttaccgcat cgcgacgggc actggccttc tggcggaaca gcgcttctgt ctctttctcg
       61 cctttcagcg ccgagatgtt ggcgatggcg ctctccagtt taaacaggaa ggcattcaga
      121 tcgatgggga tgaactgggt ggtacgaatg ctcgccagac gaccagtatc acgcagccaa
      181 cgggaagagt aatcccaacc ggaggccgct cccgcgcgta aatcgcggta cacctcgttg
      241 ggcgggcgac cagaatgttt cgcggtttca acgtcctcaa gccaggattc gtcacgcggc
      301 gtgtcgcgat cgtcccagta acggttgagc agcgatccgt ccggcatccg cacaacatgg
      361 cgataggcct gattagggat caacgattct gcaccgtcca tccagaaggc atattccatt
      421 ttaaggtggt cgagatagcg gcgcgcaccg cgtacaccat cttcttcaaa caactccacc
      481 atcagcgcaa aaaccggtgg ttgcgagcgg ctcaaataat aggtgcggtt gccgttgggg
      541 atgtgaccgt agttttcgat catccaggcg aagttatcgg ccatgcattt cagcaaatct
      601 tcccgaccac tttccgccag tcccagcatg gtgaaatagg aatcccaata gtaggtttcg
      661 ctaaaacggc cgcccgggac aatatatgac tgcggtagcg ccagcagaga agaccacgga
      721 atgtgatcct gtggttcgcg ggttagcacc ggccacagct ggtcgatatg ctctttcagg
      781 gaattttgcg ggtccgatac atactcgctg gagtagacct ccggcagcca gaagtgattt
      841 tcaacaaact tgcgcaagtc aaaatcacga tgacggcgca ctttacggta gcggattaaa
      901 atatccagcg ggtccatttt gggtgcgcag tcgggaaagg ttttactgtc ggggaaaatt
      961 ttcgccgact gaacatgctc gaacagttcg agatagcgat cggcaggcgt cagcgcatca
     1021 gaggcgggca gcccttcaat catctcgggt tccggttctg cctcgatcat ctcatccagt
     1081 tttaattcat acgggtccag ttcatagcag agatcgacct cgatcatcag ttcgtctgga
     1141 ttagggtttt gaattttctg attgagcata aggatccaac ctccgaaatt cgtcgctaat
     1201 tatcgggctt ttctttaagg gtagtcattc gctccgcctg gtgggcgaca aagcgtgcgg
     1261 tggatcacgt ttattaactt gtcaaaaaat ataacgcact aatatcaatg gaatttataa
     1321 aaatacggat ttggtgttg
//
LOCUS       DAJVPT010000274         1282 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVPT010000274 DAJVPT010000000
VERSION     DAJVPT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..579)
                     /locus_tag="OQM47_004133"
     CDS             complement(<1..579)
                     /locus_tag="OQM47_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS1482041.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLM"
     gene            949..>1282
                     /locus_tag="OQM47_004134"
     CDS             949..>1282
                     /locus_tag="OQM47_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482042.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
BASE COUNT          334 a          327 c          321 g          300 t
ORIGIN      
        1 catcagacac aggcgaactt tccagccgat atcttccagt tccgccagcg tggagagcgc
       61 ctcgtcgtac tcatcgccct gatgcccttc cataatcacc cggctcaact gctccaggtc
      121 gctatagata ttttcaattt catctgccaa ctgttcgatt ttggtttcga acagatccag
      181 cagcaactcg taggcgttac cgtctaccat cgactggcta cgggcacgca tacgatacag
      241 acgaaaagcg ggcagttcac gctcacgcag agtaaacaga cgaccatcac ggatggtaaa
      301 tgccacagtg gagttaccgg cgtgatcttc cgcatcttca aagaagaaga aggagtgaat
      361 atgcaggccg tcgtcgtctt caaagaaacg tgccgatgct tcgatgtctt ccagttccgg
      421 gcgggttgcc aggctctggc caagttcaga ttgtacgcgc agtcgctcgt cgtcgtccgg
      481 ttcgacaaga tcaatccata ctgcatttac aaggggttgt gactcttcga cttccagccg
      541 ggtcagtcgg ttattttcca gttgaaatgc gctcagcatg accgggactc ccaatgctta
      601 aaatatcgga cagttcggtg ggcacacaga aacaaattgg gtttcagacc attaaacagc
      661 ctgactcagc gcgacgggaa aaattgaggt cgctgacaac cgctaaggct atcagcaaaa
      721 agggatagcc ttaggagttg atcctggatg acaggatagt gagccagtat ctactgggtg
      781 tgtccaaggc gaatgtcctc ttagagtgat cgtgcgcgca tgttacgcca gcaaaatttt
      841 gccgtcaaca cgcaacagaa caccacagaa caatagttga acttcgcgct ttttggtact
      901 cgtcaaggtc gctaacagga taaaaaaatc ccacatctag gacctataat ggcgcatcgg
      961 ctactcattg gtaaaggaat gatcaccttg aatctcaaac ggatttttct caccctcacc
     1021 ttacttccct tgtttgctgt tgccgctgat gattgcgcac tctcagatcc gacgctgacc
     1081 gtacaggcgt ataccgttaa tcctcaaaca gagcgggtga aaatgtactg gcaaaaagcc
     1141 aatggcgaag cgtggggaac gttacatgct ctgctggcgg atattaatag tcagggtcag
     1201 gtgcagatgg cgatgaacgg cggcatctat gatgaaagct atgcgccgct cggtttgtac
     1261 atcgaaaacg gtcagcagaa gg
//
LOCUS       DAJVPT010000275         1258 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVPT010000275 DAJVPT010000000
VERSION     DAJVPT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(314..466)
                     /gene="hokE"
                     /locus_tag="OQM47_004135"
     CDS             complement(314..466)
                     /gene="hokE"
                     /locus_tag="OQM47_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1482043.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            complement(563..>1258)
                     /locus_tag="OQM47_004136"
     CDS             complement(563..>1258)
                     /locus_tag="OQM47_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCS1482044.1"
                     /translation="ENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKR
                     YRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVIS
                     YSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMS
                     RKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLT
                     PIEYRNQTYMPRV"
BASE COUNT          329 a          316 c          257 g          356 t
ORIGIN      
        1 cgagttgttg aggttcggag tggcagcccc aggattgata gaaatatctc ttggggcttc
       61 atctttccaa acctcagagt taacctgaaa ccggaaagct tcaggcaccc gccgttatct
      121 tacccttcct tagcaaaata gctgtttatt tatacagtga gtcctgtttc ggacttctgt
      181 cggcaaaact catcattgct cttaaaacga aatcgcccgc agccatcact gaccgcgggc
      241 gaaagtgtaa agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact
      301 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccctgaac tcaatattac
      361 gttccttcac cgtaaactca cacagcgagt ctccggccag aagcgtaaat cccagtaccg
      421 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattacctc
      481 catgcgaaga agggactacc atccaaattg tctggtttgg agtggcagcc ccagggtgta
      541 ctgaccccaa aaagttggac agttatacac gaggcatata ggtctgattc cgatattcaa
      601 ttggagtcag accttttaat ttcaggctaa ttcttctgct gttatagtat tcaatatatt
      661 ccgtaacagc atccttcagt tcgcttatat tactgaactc atcaagataa aaacactccg
      721 actttaaggt tccaaagaaa cactccacca cagcattatc cagacaattg ccttttctgg
      781 acatgctttg tttaatacca tgttctttaa ggatattttg atatcttctc atacgatact
      841 gccatccctg gtcagagtgc agaacaggat gctcgtgagg attaagcttt ttgaatgcct
      901 gatcgagcat attctcaacc atgttcatca ctggtctttc cgaaaggctg taagaaataa
      961 cttcgttgtt gaagagatct attactggag acaaatacag cttgcgccca ttgactgcaa
     1021 attcagtaac atcggtaacc cacttctcgt ttggccgcgt agccttgaaa tctctttgga
     1081 gaacattagg ggcggtttgc cctacctctc ctctgtaaga gcggtatcgc ttgaccttaa
     1141 tcgctgcttt aagtgagagg gttcccatca ggcgctgaac agctttatgg ttaatctgtt
     1201 tcccttctcg atgaagagac agcgttaccc tacggtatcc gtatcggcct ctattctc
//
LOCUS       DAJVPT010000276         1228 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVPT010000276 DAJVPT010000000
VERSION     DAJVPT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..629
                     /gene="agaC"
                     /locus_tag="OQM47_004137"
     CDS             <1..629
                     /gene="agaC"
                     /locus_tag="OQM47_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCS1482045.1"
                     /translation="AFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPF
                     SLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTY
                     LAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCY
                     IRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            619..>1228
                     /gene="agaD"
                     /locus_tag="OQM47_004138"
     CDS             619..>1228
                     /gene="agaD"
                     /locus_tag="OQM47_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCS1482046.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAI"
BASE COUNT          264 a          299 c          335 g          330 t
ORIGIN      
        1 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg
       61 gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc
      121 ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct
      181 cattatttat gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc
      241 ggcttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct
      301 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga
      361 cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc
      421 gcgtaatgtt caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct
      481 acatccgggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg
      541 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
      601 aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc
      661 tttcgttcgt cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt
      721 acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc
      781 gcggcgatga aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg
      841 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc
      901 aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg
      961 ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg
     1021 attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
     1081 ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct
     1141 cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat
     1201 attaacgtgg tgacatcgtt tgccatcg
//
LOCUS       DAJVPT010000277         1208 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVPT010000277 DAJVPT010000000
VERSION     DAJVPT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1069)
                     /gene="nepI"
                     /locus_tag="OQM47_004139"
     CDS             complement(<1..1069)
                     /gene="nepI"
                     /locus_tag="OQM47_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1482047.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCG"
BASE COUNT          365 a          342 c          293 g          208 t
ORIGIN      
        1 cgccacaggt attagcaagc tgaataaccg ccacctgaat agacccggct ttttctgcct
       61 gatcggccag cgagcgggtg atccacgttg accagccgac gggaaccaat gcaaaagtta
      121 gcccccagat aatcgccacg ccggtagcaa cgattttatc gcttccccac aacgtcagta
      181 ccaacgcact cacagccagt attaacggcg cgcctgctaa ggccagtttt accgaacgtt
      241 taagaatgaa cgacgaaagc gacgtaccaa taaagctggc gataccaaaa ctcaacagca
      301 ccagcgttaa gccatccacg ccgaatcccg ccaggttcat atacaccggg cgaatatacg
      361 tgaagaaagc aaactgcccg gcgaaagaca tgaagatggc gatcatccct gccatcacac
      421 ccggacgttg taataagcgg aaagtatttt gtttctgatg cgagggttcg cctggcagtg
      481 aaggcaatga tttgataatc cagaaaatac acagcacgcc catcaccgcc gccgcattaa
      541 agacattgcg ccaaccgata agctcgccta aaaaactgcc caacggcgcg gcaatcacca
      601 gcgcaataga aaccgcgccg aagatcaccg acagcgcctt cggcaccgta cgcggcggta
      661 ccagacgcat ggtcagcgac gccgacatcg cccagaaccc gcccagcgcc agccccagac
      721 aggcacgacc gattaaaagc aaactgaatg agttagcaaa ggaaaccagc aagcaggaga
      781 gcgtcagcaa aacggcaaac aaaataacaa cgtagcggcg gtcagtggcc tgaattgtct
      841 gggtaataaa caaactggca aacattgcca caaaggcggt cacggtcacc gattgcccgg
      901 caaccccttc cgaaatgcct aaatcctggg ccattggcgt caacaaactg acgggcaaaa
      961 actcaacgat aatcagacag gcgacacaaa acgccaccga gaaaacggct gaccagttcg
     1021 gtcgggtgat ggcatccgcg ccgcggtttt cggcaataaa ttcactcatg gtgttacccg
     1081 tggcatgttt caggggaaaa gccgaccact ttaacattga aatgtgtgac gaatttaacg
     1141 tttttgcaac ttttacggtg ttgcttgagc tggagttaag atcgaaacgg aacagggcaa
     1201 agattacc
//
LOCUS       DAJVPT010000278         1204 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVPT010000278 DAJVPT010000000
VERSION     DAJVPT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>1204
                     /locus_tag="OQM47_004140"
     CDS             <1..>1204
                     /locus_tag="OQM47_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1482048.1"
                     /translation="NKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVN
                     DEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNAR
                     DDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRL
                     ASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAK
                     KALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLS
                     NGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSL
                     KRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNA
                     QIQALKTLD"
BASE COUNT          365 a          282 c          287 g          270 t
ORIGIN      
        1 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
       61 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
      121 gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag
      181 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
      241 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
      301 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
      361 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
      421 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
      481 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
      541 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
      601 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
      661 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
      721 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
      781 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag
      841 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
      901 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
      961 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     1021 aactcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     1081 cggactaata accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg
     1141 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     1201 gacc
//
LOCUS       DAJVPT010000279         1189 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVPT010000279 DAJVPT010000000
VERSION     DAJVPT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            400..1149
                     /locus_tag="OQM47_004141"
     CDS             400..1149
                     /locus_tag="OQM47_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCS1482049.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          352 a          232 c          235 g          370 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcaggg
//
LOCUS       DAJVPT010000280         1165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVPT010000280 DAJVPT010000000
VERSION     DAJVPT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(100..339)
                     /locus_tag="OQM47_004142"
     CDS             complement(100..339)
                     /locus_tag="OQM47_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1482050.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(474..1079)
                     /locus_tag="OQM47_004143"
     CDS             complement(474..1079)
                     /locus_tag="OQM47_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1482051.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          330 a          265 c          199 g          371 t
ORIGIN      
        1 ttgctgccat tgaaagactc catgtaccat acaaggttgc tgccagacat attcctttac
       61 gtctgacagc ctctagagag tttttttatt ttttataagt tacggattac tcgttccgga
      121 aatcgttacc tgttgaattg ctgcagcgat agcgtcgaat gcatctttaa ttgcgcccag
      181 gaagcctgag ttctgatcgc caaagatgaa agcaagcaga gttgccatag caacaccaat
      241 caatgcatat tcgattgcag taacaccacg ttcatccttc gcgaaacgcg caaagcactg
      301 tttagtttta ccatagtact tagtaataaa agtattcatg ttttcctcgt atattattat
      361 ttaaattcaa accaccagct tcatctgcgt gtcaccggta tcccatattt ttgggatgtg
      421 gctataatta tctattcccg ctatttagtc ttcaaaaaaa attacattta atatcattta
      481 tcttgattaa gaaatagtaa gactaaaaaa acaagcgtcg tcagccagca cactgctaac
      541 acggtaagaa cgctaataaa aacactccac tgcttcgttc tataccattc aatatttttg
      601 tcagtatgtt ttattgggag cagctcttca gtagagaatt cctggctgtt atcattctca
      661 ggaatgtttg catcagtgtt tacttccctg gcctcgctat cgttggtata gctactactg
      721 tcttcatcac tgtcacgctc attctcacag acatgtttct cgacctttcc cagatatctg
      781 acgccttttc gcggaatggt tttaattaga gaactcgaca aacctgcgac gtctaatgcc
      841 tttctaagca tgtaaatctg cccataataa ctgctgtcgc ttaccgaacc tttctgctcc
      901 cgccaaacct cattgataat atgttgctta tcggattctc cctgtagtaa aaggagtaaa
      961 aatcgatagc cgttttcaga cagattgatt acattgcctg tactggtggt gatacgtcga
     1021 gacgctgggt aaaacttaac tccctgccct aaagaaaaat aaaaacggtc atcgctcata
     1081 agtaccgacc cctgttttct tcttcacatg actactcatt acaactttgt atgaagtaag
     1141 atgcaaaatc atattgttta catta
//
LOCUS       DAJVPT010000281         1143 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVPT010000281 DAJVPT010000000
VERSION     DAJVPT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..375
                     /gene="curA"
                     /locus_tag="OQM47_004144"
     CDS             <1..375
                     /gene="curA"
                     /locus_tag="OQM47_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1482052.1"
                     /translation="YYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRL
                     PLLMATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQ
                     TFIGLLKGKNFGKVVIRVAGDD"
     gene            573..>1143
                     /gene="mcbR"
                     /locus_tag="OQM47_004145"
     CDS             573..>1143
                     /gene="mcbR"
                     /locus_tag="OQM47_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1482053.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLA"
BASE COUNT          334 a          236 c          281 g          292 t
ORIGIN      
        1 tattatgaaa acgtgggcgg taaggtattt gatgcagtgc taccattact taatacatct
       61 gcgcgcattc ccgtttgtgg cttagtgagc agctataacg ctacagagct accacccggt
      121 ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa aacgtattcg cttgcaaggg
      181 tttattatcg ctcaggatta tggtcaccgc atccatgagt ttcagaagga gatggggcaa
      241 tgggtgaaag aggataaaat ccactaccgc gaagatatta ctgatggttt agagaatgcg
      301 ccacagacgt ttatcggcct gctgaagggt aaaaacttcg gcaaagtggt gatccgcgtc
      361 gcgggtgatg attaaagttt caacggcggc gtaagccgcc ataaaaatcc aggcaaaaag
      421 cttattttca gctttaatta actaaccacg tcattgttct gctgctaata acaacaaagg
      481 tgggtggttt atgattattc actttaatac actaggtgaa ctccttctgc catgcaggca
      541 gggttggaca gaaaacacgc gaaggaatta aaatgcctgg aacgggaaaa atgaaacatg
      601 tcagtttgac tctgcaggtt gagaacgacc tgaaacatca gcttagtatt ggcgcattaa
      661 aacctggcgc acgcctgatt actaaaaatc tggcggagca attaggtatg agtattacac
      721 ctgtgcgtga agcattatta cgtctggttt cggtgaatgc gctttctgtc gcacctgcac
      781 aagcatttac agtaccggaa gtgaggcaaa gtcagttaga cgaaatcaat cggatccgct
      841 acgaactgga attaatggca gttgccctgg ctgttgaaaa cctctcctcg caagacatag
      901 cagaacttca ggaattgctc gggaaattac agcaggcgca agaaaagggc gacatggaac
      961 aaatcattaa tgtgaacagg ctatttcgct tagcgattta tcatcgctca aatatgccca
     1021 tcctgtgtga gatgattgag caactgtgga tcaggatggg acctgcttta cattatcttt
     1081 atgaagcgat taacccagcg gaattacggg agcgcataga aaactatcat ctattactcg
     1141 ctg
//
LOCUS       DAJVPT010000282         1103 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVPT010000282 DAJVPT010000000
VERSION     DAJVPT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..517)
                     /locus_tag="OQM47_004146"
     CDS             complement(<1..517)
                     /locus_tag="OQM47_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1482054.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNAR"
     gene            655..>1103
                     /gene="pgrR"
                     /locus_tag="OQM47_004147"
     CDS             655..>1103
                     /gene="pgrR"
                     /locus_tag="OQM47_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1482055.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTG"
BASE COUNT          276 a          253 c          262 g          312 t
ORIGIN      
        1 ttcgggcgtt tgagccagct tcaatgtgtg gcagagcatc aatagatttc acattgtttt
       61 cccaaccatt gcggaacatc gaaccaatat tgaccgcact aacggtaccg atggccttga
      121 tgcggcgatc ctgaattgca gcattggctg tatatcctgc accggcacaa attcccatcg
      181 caccaattcg ggtattgtcg acataagaaa gcgttgtcag gtaatcaatc acggcactga
      241 cgtcttcagt acgaatgtat gggttttcta actgacgcgg ctcgccgcca ctttcacctt
      301 gataagatgc gtcataagca atagtgacaa atcccttttc cgccagcttt tcggcatagg
      361 ttccggccgt ttgctcttta acgcccccac ctgggtgaga taccacaatt gccggatact
      421 gacgggtttc atcaaatttt ggagggaaat agatcactgc agacaaagag atggttggat
      481 tattgctgtt agtgaagctg actttattat tcatcattca gttcctcatg gagttgtcgg
      541 ttcgttttga cggttggtga tatcactata gatattgatc attaagttga ttagacccta
      601 aatcatgatt agacctatca tttaaatgat taatagattg tatggagata ctgaatgaag
      661 cgtgaagaaa tcgctgatct gatggcgttt gtcgtcgttg cagaggagcg tagcttcact
      721 cgcgcagcag cccgcctgag catggtgcag tcagctttaa gccagatagt gcgccgtata
      781 gaagaacgat tgggattgcg gcttctgacg cgaaccacgc gcagcgttgt acccactgaa
      841 gcgggcgagc atcttttgtc tgttcttggc cctatattgc atgacataga ttctgccctg
      901 acatccctga gcgatctgca gaaccgccca tccgggacaa tacgtattac tactgtagaa
      961 catgcagcaa aaacgatatt gttaccagcc atgcgcacat tcctgaaatc gcatcctgaa
     1021 attgatattc agcttaccat tgattatggt ttgaccgatg tcgtttctga acgttttgat
     1081 gcaggcgtcc tgaggtgtac tgg
//
LOCUS       DAJVPT010000283         1101 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVPT010000283 DAJVPT010000000
VERSION     DAJVPT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            136..219
                     /gene="idlP"
                     /locus_tag="OQM47_004148"
     CDS             136..219
                     /gene="idlP"
                     /locus_tag="OQM47_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1482056.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            216..608
                     /gene="iraD"
                     /locus_tag="OQM47_004149"
     CDS             216..608
                     /gene="iraD"
                     /locus_tag="OQM47_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1482057.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(601..>1101)
                     /gene="hypT"
                     /locus_tag="OQM47_004150"
                     /gene_synonym="qseD"
     CDS             complement(601..>1101)
                     /gene="hypT"
                     /locus_tag="OQM47_004150"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1482058.1"
                     /translation="EAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCASD
                     EHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVAL
                     DGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRE
                     LEIVLS"
BASE COUNT          285 a          259 c          276 g          281 t
ORIGIN      
        1 tgatttttat caaatcatga cttttttcct gaaaagtcag tagatattca taggcaagta
       61 aggttttata ctttgctgac aggattcagg cctgtctcag actgacatgg atgtaatgaa
      121 caaaagggaa tggctatgga aaatgagcat caatacagtg gtgcccggtg ttcagggcaa
      181 gccgcatatg ttgctaaacg tcaggagtgc gcaaaatgat gcgccaatca cttcaggctg
      241 ttttacctga aatttcaggc aataaaacgt cattgctgcg taaatcggta tgcagcgatc
      301 tcctcactct ttttaattct cctcattcgg cattaccgtc attacttgtt tcaggcatgc
      361 ccgagtggca agtgcataac ccgagcgaca aacatctcca gagctggtat tgccgtcagc
      421 tgcgttctgc actgttattt catgagccac ggattgcggc gttgcaggtt aatttgaaag
      481 aagcgtactg ccacactctg gcgatcagcc ttgagataat gctgtaccac gacgatgaac
      541 cgctaacgtt tgatctggtc tgggataatg gcggttggcg cagtgcgacg cttgagaatg
      601 tcagctaagc acaatctcca gctcgcgcag ttcacgccag aagcgttcgg caactggatt
      661 catgcgggta ttcatccggt atgcgtaggc ctgaatcggg atcacctgtt cgtcccgatt
      721 cagcacaacg agctgcccgc tgcgaatttc ttgttggatg gcatactccg gcagccaggc
      781 aatcccacag ccgtcgaggg caacctgctt taaaagctcg ctcatcgaag agacaaaaaa
      841 ggtgctgaaa cttaactcac tgtggcgcgt cagggtgcga ttaatcaatc gcaccatgta
      901 ggagttgcgg ctgtaattca gtaacggaaa gtgtggctgc gcgaggttaa aaagtgcttc
      961 tccgtgttcg tcactggcgc agacagggaa caattgagat tcaaataagc gaatgtggtc
     1021 aaacggcgct tccagcaaat cttcgtcgtg aaaggaaaaa atacagtcac tttgcccttc
     1081 acgcagttta tcgaccgctt c
//
LOCUS       DAJVPT010000284         1078 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVPT010000284 DAJVPT010000000
VERSION     DAJVPT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..1078
                     /gene="tcyP"
                     /locus_tag="OQM47_004151"
                     /pseudo
     CDS             1..1078
                     /gene="tcyP"
                     /locus_tag="OQM47_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT          204 a          271 c          293 g          310 t
ORIGIN      
        1 cataccattt atggttctga cagccaggta cttaaagatt ctgtacagtg gtttaacatc
       61 gttggtaacg gctatgttca actgctgcaa atgatcgtta tgccgttagt cttcgcctct
      121 attctgagcg cggttgcccg tctgcataac gcatctcagt taggcaaaat cagttttctg
      181 accatcggta cgcttttgtt taccacgctg attgcggcgc tggtcggtgt gctggtcacc
      241 aacctgtttg gtttgacggc tgaaggtctg gttcagggtg gtgcagaaac tgcacgtctg
      301 aacgccattg aaagtaacta tgttggtaaa gtctctgatc agagcgttcc gcagctggtc
      361 ttgtccttta tcccgaaaaa cccgtttgcc gatcttaccg gagccaatcc gacgtcaatt
      421 atcagcgtgg taatttttgc cgcattcctc ggcgtagctg cattgaaact gctgaaggat
      481 gatgcgccga aaggggaacg cgtcttaacc gctatcgata ccctacaaag ctgggtgatg
      541 aaactggttc gccttgtcat gcagttgacc ccttacggcg ttctggctct aatgaccaaa
      601 gtggttgcag gttctaactt gcaagacatc atcaaactgg gaagtttcgt tgtcgcgtcc
      661 tacctcggtc tgctgattat gtttgctgtg catggcattc tgctgggcat taatggcgtg
      721 agtccgctga agtacttccg taaggtatgg cctgtgctga cgtttgcctt taccagccgt
      781 tccagtgctg cgtctatccc actgaatgtg gaagcacaaa cgcgtcgtct gggcgttcct
      841 gaatccatcg ccagtttcgc cgcctctttc ggtgcaacca ttggtcagaa cggctgcgcc
      901 ggtttgtatc cggcaatgct gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg
      961 acccgatgtg gattgcgacg ctggtcggta ttgttaccgt tagttccgca ggcgttgccg
     1021 gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgcc
//
LOCUS       DAJVPT010000285         1077 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVPT010000285 DAJVPT010000000
VERSION     DAJVPT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>1077
                     /locus_tag="OQM47_004152"
     CDS             <1..>1077
                     /locus_tag="OQM47_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCS1482059.1"
                     /translation="IGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETS
                     RLWGRIAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKV
                     NYSQIDSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDE
                     TLFEFYDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNF
                     WHQGNLKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKS
                     LPKPVRRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPD
                     HLKITFRVVDDKNKKLKEGRSLQDL"
BASE COUNT          266 a          270 c          306 g          235 t
ORIGIN      
        1 tatcggcatg aaagatgccg ataaacagga atataccggt gcacgtaacg cgcgtttctc
       61 catcttcccc ggttctggtt tattcaaaaa gccgccgaaa tgggtaatgg tggcggaact
      121 ggtagaaacc agccgcctgt gggggcgcat tgctgcgcgt atcgacccgg aatgggtaga
      181 acccgttgct cagcatttga ttaaacgcac ctacagcgaa ccgcactggg aacgggcgca
      241 gggcgcggtg atggcaacgg aaaaagtcac tgtttatggt ttgccgattg ttgctgcgcg
      301 caaggtcaac tacagccaga tcgattcggc gttatgtcgt gaactcttta ttcgccacgc
      361 tctggtggaa ggtgactggc agacgcgtca cgcattcttc cgtgaaaacc tgaaactgcg
      421 ggccgaagtg gaagagctgg aacacaaatc acgtcgccgc gatattctgg ttgatgacga
      481 aacgctgttt gagttctacg accagcgcat cagccacgat gtaatctccg ctcgtcactt
      541 cgatagctgg tggaaaaaag tcagccgcga aacgcctgat ttgctcaact ttgaaaagag
      601 catgttgatc aaagaagggg cggaaaaaat cagcaagctg gattacccga acttctggca
      661 tcagggcaat ctcaagctgc gtttgagcta tcagtttgag ccaggcgcgg acgcggacgg
      721 tgtgaccgta catattccgc tgccgttact taaccaggtt gaggaaagcg ggtttgaatg
      781 gcagatcccc ggtctgcgcc gcgaattggt gattgctctg attaaatcgt tgccgaaacc
      841 ggtacgccgt aattttgtgc ctgcaccaaa ctacgccgaa gcgtttttag gccgcgtcac
      901 accgctggag ttaccgttgc tcgacagcct tgagcgcgag ttacggcgga tgaccggcgt
      961 taccgttgac cgcgaagact ggcactggga tcaggtgccc gatcacctga aaatcacctt
     1021 ccgcgtggtg gatgacaaaa acaagaagct aaaagaaggg cgctcgctac aagatct
//
LOCUS       DAJVPT010000286         1069 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVPT010000286 DAJVPT010000000
VERSION     DAJVPT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(211..375)
                     /locus_tag="OQM47_004153"
     CDS             complement(211..375)
                     /locus_tag="OQM47_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482060.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
BASE COUNT          262 a          226 c          349 g          232 t
ORIGIN      
        1 tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg agccggttac
       61 tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg tctgcaatac
      121 cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat agctgatgga
      181 gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg cgtttccggc
      241 gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg tatgagagat
      301 gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt taagagcggt
      361 ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt tgcggagatg
      421 aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt cgaaaatgtg
      481 ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg cggcatccgg
      541 catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac ctgtcgtctc
      601 gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc gatgcgagac
      661 acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg gagcgaaggg
      721 ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc ccatgagtca
      781 gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc gggtcaaagc
      841 taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg ttaccaatcg
      901 aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat tgcccgcaaa
      961 cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg aattcgtcac
     1021 gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaa
//
LOCUS       DAJVPT010000287         1064 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVPT010000287 DAJVPT010000000
VERSION     DAJVPT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            24..>1064
                     /locus_tag="OQM47_004154"
     CDS             join(24..273,273..>1064)
                     /locus_tag="OQM47_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCS1482061.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAH"
BASE COUNT          291 a          262 c          301 g          210 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttgaaag
      661 gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt gatgtgacct atatctggac
      721 gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg
      781 ctgggccatg tcgttctcgc cggacagcag gctcaccatg aaagcgctgg aaatggcatg
      841 ggaaacccgt ggtaagcccg gcggggtgat gttccacagc gatcagggca gtcattatac
      901 gagcaggcag ttccggcagt tattgtggcg ataccagatc agacagagta tgagccggcg
      961 cggaaactgc tgggataaca gcccaatgga acgcttcttc aggagtctga agaacgaatg
     1021 gatgccggtg gtgggttacg taagcttcag cgaggcagct cacg
//
LOCUS       DAJVPT010000288         1055 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVPT010000288 DAJVPT010000000
VERSION     DAJVPT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(321..686)
                     /locus_tag="OQM47_004155"
     CDS             complement(321..686)
                     /locus_tag="OQM47_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1482062.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(686..922)
                     /locus_tag="OQM47_004156"
                     /pseudo
     CDS             complement(686..922)
                     /locus_tag="OQM47_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          267 a          286 c          227 g          275 t
ORIGIN      
        1 agtggcatgc ccgaggcaga cagcctcaag cacccgcagc aatcttaccg ataccgataa
       61 gaaaacgcta ttttttattg ccagcacctt ctatccaggc taatttatct gcgtcagaac
      121 ggcgcaatgc gctcgcctga gatacgttta cttgccatgg taacaacagg taacggcaat
      181 tgctgtttct gttttgtttc cttcaaaaac cccggaccat caatccgggg tttttgtttg
      241 ttaaccccaa cggcaaatcg aatacaccac cagcgccacc gccatcgcaa ttcctaccgt
      301 ggtgaatgcc tcaggccagg tcattgattc acctcctgcg gtggttccgg tagcggcatc
      361 cagtgagttg agctctctac ttcaacgccg tctttgtcca caaaagccat cttattcccg
      421 ccacgagtgg ggcaaaaaac cttatcccag gaaccgggga atacattccc gagatcatcg
      481 agaagaatca catcgcaata ttcagcagga actgcatcac tacagcttat ccagccagcc
      541 agagttaccg gagagttgcc agccttacgc atggccattt ccacgatttc aaccatatct
      601 cctggtggaa ttttacaaag ttgcccaata catttctgct gcctggcata ttcgagaatg
      661 tgctccagtt tggttcgatt aatcattatt tatctccctt aagcatggca gcgcggcagg
      721 cgttccagcc atcaacataa tcaaacgtat tactatcgtt tggcccgatt tcatccggca
      781 ctatcggctc tggttggata gtgacgttgg caaaggcagc acgcaaaccg gttttaattt
      841 cttcgatttc atcagagcca agcgatgaat ctgacagtgc gtgatggaat gcgtaagcca
      901 tgtcgtcgtt tactgcaacc ggcactgccg gcgctgcgta aagcggtgtt atatctgccc
      961 gaaaatcaca tgctttatgc agtcgtaccc aacgctcaac ttccgctttg taaaaatata
     1021 tagcagtgaa cgtattagat tcctggtcaa cttga
//
LOCUS       DAJVPT010000289         1042 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVPT010000289 DAJVPT010000000
VERSION     DAJVPT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(228..653)
                     /locus_tag="OQM47_004157"
     CDS             complement(228..653)
                     /locus_tag="OQM47_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482063.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(650..1033)
                     /locus_tag="OQM47_004158"
     CDS             complement(650..1033)
                     /locus_tag="OQM47_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482064.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          246 a          296 c          258 g          242 t
ORIGIN      
        1 tctcgcgtat ggttgattga caccacagtg cgaattcggc gaatgcgaga tcacggtacg
       61 attccgcacc acactaccaa tcaatacccc gggttttatt caggtaagca ggactgcgga
      121 tatccggtgt cgcgcctttg tgtcacgaac ggggtgggtg cgaaacacca gataaaatac
      181 aggttggcaa tacctctgaa cgccctgagc aggtcgggca tttcggatta agtactcgca
      241 cctccgcagt cctcaaacaa gtctggctgg tagctgtaaa cagacttcgt acataccgct
      301 ctgcaataaa tccccgtacc acaggcctca cagaacttct tcccatgaaa aaactgctca
      361 cacatcacac aatgccactc cagcacgacc ggtaatggcg ataaaaacat cgccatatcc
      421 tcaatgtaag ggtgggactt ctccggattc agcaccacac agtccgcctt ctgttgctca
      481 ctcagggcat gtaaatcatg ctcaaaccac gccccctgag catctgtctg cagaatcaac
      541 cggccatggc aggaaaggca gaaacactgc cggatatttc tgctgagcgc agagtcggca
      601 ctgataatgt gctcacccgg cgtgatcccc agccccgttt ttataccgtt cattctgctc
      661 ccccctcctc actgaagcgc ccggtctggc agtgtgtgca gtactgctcc ccataataat
      721 cgtggtgaca ttgcctgcag tgccagctgg ctttacgtac tacgggtaag gtatccggga
      781 cgaattgctg cagacgttta atcaaccgta tttctctgcg ttccggcctg acataagggc
      841 actcatgacc gtgctccgtc agcccttcgt cagcgtgttc aaaccagggc cgttcagtgt
      901 cgtattgcgg gtggtatctg agtgcactgc cgcaaaggtg gcaggtgtag ctatcgtaag
      961 gtgcagtctg tgcggtacgg gcaccggtca gacgtccgtt gccatcaagt gcgagaaaag
     1021 attttgcgta catagtatat gt
//
LOCUS       DAJVPT010000290         1041 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVPT010000290 DAJVPT010000000
VERSION     DAJVPT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>1041)
                     /gene="btsS"
                     /locus_tag="OQM47_004159"
     CDS             complement(<1..>1041)
                     /gene="btsS"
                     /locus_tag="OQM47_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1482065.1"
                     /translation="TVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGF
                     NEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETG
                     EVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTL
                     GEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQ
                     ASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELS
                     QQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNA
                     SGLGMNLVDKRLR"
BASE COUNT          248 a          273 c          250 g          270 t
ORIGIN      
        1 acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata
       61 caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc gggcgctgat
      121 tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt tttccactat
      181 cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatgtt
      241 gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt tcacatgttc
      301 aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt
      361 ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca ccgctttaat
      421 ggtgttaagc gcattaaaca aaaaatgagg attcacctgg gcgtgaagca gtttgatttc
      481 tgactgggtg agcatcgctt tttgccgctc atattgccct gcaagaatct gcgccgaaag
      541 cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa ctgaataacc ggtttttggc
      601 ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg ccacgcaacg gaattaccag
      661 cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt cgttgccatc
      721 agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag aaatcggttt
      781 gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt
      841 aatcgcgact gcaccaatat ccagctcctg atacagtacc tgagccactt tcatgctgtt
      901 cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag gctgccactt tcagcgcagt
      961 ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat
     1021 aaacagcgcc gcgccgacgg t
//
LOCUS       DAJVPT010000291         1004 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVPT010000291 DAJVPT010000000
VERSION     DAJVPT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>1004
                     /locus_tag="OQM47_004160"
     CDS             <1..>1004
                     /locus_tag="OQM47_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="involved in the post-transcriptional processing of
                     the daa operon mRNA, which encodes proteins involved in
                     fimbrial biogenesis of an enteropathogenic E. coli strain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1482066.1"
                     /translation="RDQLQAIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNL
                     RHTEILPLYARLSNSEQNRVFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISR
                     YSYRTKVQRLPIEPISQASANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRT
                     NLASVILQMTALGLGDIAAFPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPL
                     GRQLSQLPVDPRLARMVLEAQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRR
                     FHDKESDFLAFVNLWNYLGEQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVV
                     "
BASE COUNT          220 a          294 c          277 g          213 t
ORIGIN      
        1 cgcgatcagt tgcaggcgat ttttgacgcc gtagacgaac taagccagga aagccccggc
       61 gacattctga tctttatgag cggcgagcgg gaaattcgcg ataccgccga tgcgctgaac
      121 aagctgaact tacgccatac cgaaatcttg ccgctttatg cgcggctttc gaacagcgaa
      181 cagaaccgcg tgttccagtc gcacagcgga cggcgcattg tgctggcgac caacgtcgcg
      241 gaaacctcgc tgaccgtacc ggggattaaa tacgtcatcg accccggtac agcgcgtatc
      301 agccgctaca gctatcgcac caaagtgcag cgtttgccga ttgaacccat ttcgcaggcg
      361 tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg tattcgtctc
      421 tattccgaag acgatttcct ctcgcgcccg gagtttaccg atccggagat tctgcgtacc
      481 aacctggcct cggttatttt gcagatgacc gcgctggggc tgggcgatat cgctgcgttc
      541 ccgtttgtcg aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa
      601 ctgggcgcga tcaccacgga tgaacaggcc agcgcctata aactgacgcc gctcggtcgc
      661 cagctctcgc agttgcctgt cgacccacgt ctggcgcgta tggtgctgga agcgcaaaaa
      721 catggctgcg tgcgtgaggc gatgattatc acgtccgcgc tctccatcca ggatccgcgc
      781 gaacgtccga tggacaaaca gcaggcatcg gacgaaaaac atcgtcgctt ccacgacaaa
      841 gaatccgact tcctcgcgtt tgtaaatctg tggaattatc ttggcgagca gcaaaaggcg
      901 ctttcttcca acgccttccg tcgcctgtgt cgtaccgatt acctcaacta tctgcgcgtg
      961 cgcgaatggc aggatatcta cacccagttg cgccaggtgg tgaa
//
LOCUS       DAJVPT010000292          997 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVPT010000292 DAJVPT010000000
VERSION     DAJVPT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>997
                     /gene="gmd"
                     /locus_tag="OQM47_004161"
     CDS             <1..>997
                     /gene="gmd"
                     /locus_tag="OQM47_004161"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1482067.1"
                     /translation="SSFNTERVDHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQP
                     DEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYG
                     LVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMYACNGILFNHESPRRGETF
                     VTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATG
                     VQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYF
                     RPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHSLLKSHGYDVA"
BASE COUNT          241 a          281 c          265 g          210 t
ORIGIN      
        1 catcgtcatt caacaccgag cgcgtggatc acatttatca ggatccgcac acctgcaacc
       61 cgaaattcca tctgcattat ggcgacctga gtgatacctc taacctgacg cgcattttgc
      121 gtgaagtaca gccggatgaa gtgtacaacc taggcgcaat gagccacgtt gcggtctctt
      181 ttgagtcacc agaatatacc gctgacgtcg acgcgatggg tacgctgcgc ctgctggagg
      241 cgatccgctt cctcggtctg gaaaagaaaa ctcgtttcta tcaggcttcc acctctgaac
      301 tgtatggtct ggtgcaggaa attccgcaga aagaaaccac gccgttctac ccgcgatctc
      361 cgtatgcggt cgccaaactg tacgcctact ggatcaccgt taactaccgc gaatcctacg
      421 gcatgtacgc ttgtaacgga attctcttca accatgaatc cccgcgccgc ggcgaaacct
      481 tcgttacccg caaaatcacc cgcgcaatcg ccaacatcgc ccaggggctg gagtcgtgcc
      541 tgtacctcgg caatatggat tccctgcgtg actggggcca cgccaaagac tacgtaaaaa
      601 tgcagtggat gatgctgcaa caggaacagc cggaagattt cgttatcgcg accggcgttc
      661 agtactccgt acgtcagttt gtggaaatgg cggcagcaca gctgggcatc aaactgcgct
      721 ttgaaggcac gggtgttgaa gagaagggca ttgtggtttc cgtcaccggg catgacgcgc
      781 cgggcgttaa accgggtgat gtgattatcg ccgttgatcc gcgttacttc cgtccggcag
      841 aagttgaaac gctgctcggc gacccgacca aagcgcacga aaaactgggc tggaaaccgg
      901 aaatcaccct cagagagatg gtgtctgaaa tggtggctaa tgacctcgaa gcggcgaaaa
      961 aacactctct gctgaaatct cacggctacg acgtggc
//
LOCUS       DAJVPT010000293          975 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVPT010000293 DAJVPT010000000
VERSION     DAJVPT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OQM47_004162"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OQM47_004162"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1482068.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          258 a          219 c          218 g          280 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ataag
//
LOCUS       DAJVPT010000294          971 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVPT010000294 DAJVPT010000000
VERSION     DAJVPT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..950)
                     /locus_tag="OQM47_004163"
     CDS             complement(<1..950)
                     /locus_tag="OQM47_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1482069.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNTTHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGF"
BASE COUNT          223 a          286 c          241 g          221 t
ORIGIN      
        1 aaaaaaccgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatgaact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggtattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtaagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac gggggagcat cagaagggcg
      961 gggcgactcc g
//
LOCUS       DAJVPT010000295          970 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVPT010000295 DAJVPT010000000
VERSION     DAJVPT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..181)
                     /locus_tag="OQM47_004164"
     CDS             complement(<1..181)
                     /locus_tag="OQM47_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1482070.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKL"
     gene            complement(168..539)
                     /locus_tag="OQM47_004165"
     CDS             complement(168..539)
                     /locus_tag="OQM47_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1482071.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(551..952)
                     /locus_tag="OQM47_004166"
     CDS             complement(551..952)
                     /locus_tag="OQM47_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1482072.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          222 a          303 c          213 g          232 t
ORIGIN      
        1 ccagtttcag ggggatccct ttcacctggt tatcgcccgc aacggtattc ccggcaacct
       61 gccgggtggc aaccgagacc gctttctgtg ccacacggtt tatcgcccat gcgctggcct
      121 gtggcaccat acgggtatca aggctgttca gattgcggat ggcattctca agccccttca
      181 taatatcgtc ctgcccttac gtttcccggc agcccctcac gccgcaaccg gatataacta
      241 catccgccat catcaggggt gatacgatcc acacagaaag actcacgacc aatcatcagc
      301 gaatcattgc gacacagccg taaaatatct gctgttttca caaacaggga tggtaaagag
      361 tcttcaatac gcacaccgcc ggccaccaga gaaatatttt caggatcatc aaaaaccccg
      421 gaaatcatga tcccttttag ccggcctgac gtaatgaccg ctctaatccc catattgcga
      481 ataatggcct tgtctgcgag atcaagtgca acatcaaaca gattatcgaa atcagccacg
      541 ctgcctcccg ttattgcatt ctggccaggc cacgttctgt cattccggct gccacaccgg
      601 caaagacacg gaacgccatt cccggcggca caaatgccag aggttcatcc cgcgtggcat
      661 gaagtgcatc ggtatgcagc gtcaccagtg ccacgactgt aaccagatca tccatattct
      721 gaatcacggt ctccggctgc gctgatatca cctcattttc atgtccggtc agcgcatttt
      781 ccgggtgggc agacttatcc cggatgtcag tgtcatccat attggcaagt tcctcctcca
      841 gctcagccac acgctgcgca atatctgctg cgcttcccga gatatccggc tcacgtccca
      901 gcgatgctgc cagttctctc agacgtttca ggttatcgtc tttctttgcc atatctgttc
      961 ctgtccgaat
//
LOCUS       DAJVPT010000296          970 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVPT010000296 DAJVPT010000000
VERSION     DAJVPT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..102
                     /locus_tag="OQM47_004167"
     CDS             <1..102
                     /locus_tag="OQM47_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482073.1"
                     /translation="CELVLPAGTLRLKGKLTPALLQILIREIKGSSH"
     gene            102..449
                     /gene="tnpB"
                     /locus_tag="OQM47_004168"
     CDS             102..449
                     /gene="tnpB"
                     /locus_tag="OQM47_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482074.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            469..>970
                     /locus_tag="OQM47_004169"
     CDS             469..>970
                     /locus_tag="OQM47_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482075.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLA"
BASE COUNT          230 a          270 c          270 g          200 t
ORIGIN      
        1 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
       61 ttacagatac ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag
      121 gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg
      181 tctcaaaagt tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc
      241 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
      301 ttaccagacg cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc
      361 accttactcc ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac
      421 gaacggaacg cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
      481 cttgctcatg agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
      541 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
      601 aaccggctga aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa
      661 aaacttcgcg caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt
      721 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg
      781 cgccagtctt cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc
      841 cgtccggaag aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat
      901 gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
      961 aaactggcct
//
LOCUS       DAJVPT010000297          955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVPT010000297 DAJVPT010000000
VERSION     DAJVPT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(4..697)
                     /gene="ospZ"
                     /locus_tag="OQM47_004170"
                     /pseudo
     CDS             complement(4..697)
                     /gene="ospZ"
                     /locus_tag="OQM47_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            753..905
                     /locus_tag="OQM47_004171"
                     /pseudo
     CDS             753..905
                     /locus_tag="OQM47_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          285 a          193 c          159 g          318 t
ORIGIN      
        1 acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca tttgttttat
       61 gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac gagcaataat
      121 aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact tgcataatct
      181 ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct tgggaagaat
      241 catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag aagatcttac
      301 aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa ctatccacag
      361 aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct tccggtgtac
      421 tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac attgtcttca
      481 tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat tgtgccatac
      541 ttctggttat accagagata tacttcttgt catatatgtt tggtgattca ttaaagtaac
      601 catgttcaac tctaggataa atatttctca ctatatattc agtatttgca gtatttattg
      661 gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata ctcaaatata
      721 aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga gaacacttta
      781 tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat tacaatcgct
      841 ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac cagaacctcg
      901 cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac gactg
//
LOCUS       DAJVPT010000298          945 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVPT010000298 DAJVPT010000000
VERSION     DAJVPT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..894
                     /gene="lysC"
                     /locus_tag="OQM47_004172"
     CDS             <1..894
                     /gene="lysC"
                     /locus_tag="OQM47_004172"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1482076.1"
                     /translation="GRAEPDVAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTL
                     GRGGSDYTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATF
                     GAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLT
                     LHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTSTGDALLTQSL
                     LMELSALCRVEVEEGLALVALIGNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLC
                     FLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          204 a          252 c          265 g          224 t
ORIGIN      
        1 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
       61 cgcctcaatg aaggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
      121 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
      181 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
      241 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
      301 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
      361 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
      421 actgaaaatc cgccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
      481 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
      541 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
      601 cttgatacca ccggttcaac ctccactggc gatgcgttgc tgacgcaatc tctgctgatg
      661 gagctttccg cactgtgccg ggtggaggtg gaagaaggtc tggcgctggt cgcgttgatt
      721 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
      781 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
      841 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
      901 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtca
//
LOCUS       DAJVPT010000299          932 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVPT010000299 DAJVPT010000000
VERSION     DAJVPT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..423
                     /locus_tag="OQM47_004173"
     CDS             <1..423
                     /locus_tag="OQM47_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1482077.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            471..791
                     /gene="cyaY"
                     /locus_tag="OQM47_004174"
     CDS             471..791
                     /gene="cyaY"
                     /locus_tag="OQM47_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1482078.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT          262 a          230 c          233 g          207 t
ORIGIN      
        1 caattccatc tgattactgg caatattgcg caatataaag atcggctgta tgtaacaaaa
       61 ttatcgacat ttggtgaaga ccagcttgag ataatcccgc tggatacgcc agacctggtc
      121 attgatcgct catttaatag cggcaaacag catgcttact ttatccgcca attacggtca
      181 aagagcttgc aaattattcc agttaacggt tcgctaacta aaaacgatcg cttcgcttat
      241 gacgatcgca atgtttatac atggaccgat acagaggtaa ggattacgcc ctccccctgc
      301 ccggcgaaaa ctcgtgtcag agaggagaac gtacgtgaag ttcaaaacag agacatcatt
      361 attccggtga cggatgaatc atgccggaac gcagcagcag aggtgcaaac tttgaagccc
      421 tgaccccatg ccgctatact gccgccatca cttaaaaaca ggatacaaca atgaacgaca
      481 gtgaatttca tcgcctggct gatcaactgt ggctgaccat tgaagagcgt ctggacgaca
      541 gggatggcga cagcgatatc gactgcgaaa tcaacggcgg cgtactgacc attacctttg
      601 aaaacggcag caaaatcatt attaaccgcc aggagccgct gcaccaggtg tggctggcaa
      661 ccaaacaggg cggctaccat tttgacctga aaggcgatga atggatttgc gatcgcagcg
      721 gcgaaacctt ctgggatttg ctggaacagg cggcgacgca acaggcgggt gaaacagtca
      781 gtttccgcta agattgcatg ccggataagc ctcgctttcc ggcacgctca tcacgaaaaa
      841 tactgctgta atagcggcgt atcgtgatcc tgattggcgg gcggcaggtt gccaatcgat
      901 tttgttcgga acggaatcac ctgttcgcga cc
//
LOCUS       DAJVPT010000300          932 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVPT010000300 DAJVPT010000000
VERSION     DAJVPT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..905
                     /gene="argH"
                     /locus_tag="OQM47_004175"
     CDS             <1..905
                     /gene="argH"
                     /locus_tag="OQM47_004175"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1482079.1"
                     /translation="QRAQPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALA
                     GTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFF
                     NTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAY
                     NKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLV
                     AKGVPFREAHHIVGEAVVEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEK
                     RAAKGGVSPQQVAQAIAFAQARLE"
BASE COUNT          191 a          224 c          301 g          216 t
ORIGIN      
        1 tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgctgg
       61 cgcgtgatga aagccgtttg caggatgcgc ttaagcgtct ggatgtcagc ccattaggct
      121 gtggcgcgct ggcgggaacg gcctatgaaa tcgaccgtga acagttagca ggctggctgg
      181 gctttgcttc ggcgacccgt aacagtctgg acagcgtttc tgaccgtgac catgtgttgg
      241 aactgctttc tgctgccgct atcggcatgg tgcatctgtc gcgttttgct gaagatctga
      301 ttttctttaa caccggcgaa gcggggtttg tggagctttc ggaccgcgtg acttccggtt
      361 catcattaat gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg
      421 gccgggtgca gggtgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt
      481 acaacaaaga tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg
      541 actgcctgca tatggcggcg ctggtactgg acggcattca ggtgaaacgt ccgcgttgtc
      601 aggaagcggc gcagcagggg tatgcgaacg ccactgaact ggcggattac ctggtggcga
      661 aaggcgtacc tttccgcgag gcgcaccata ttgtgggtga agcggtggtg gaagccattc
      721 gtcagggcaa accgctggaa gatctgccac tcagtgagtt gcagaaattc agtcaggtga
      781 ttggcgaaga tgtctatccg attctgtcgc tacaatcgtg cctcgaaaag cgtgcggcaa
      841 aaggcggcgt ctcaccgcag caggtggcgc aggcgattgc ttttgcgcag gctcggttag
      901 agtaatttgt ttatattgct cccctgaaaa tg
//
LOCUS       DAJVPT010000301          927 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVPT010000301 DAJVPT010000000
VERSION     DAJVPT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..541)
                     /gene="uidR"
                     /locus_tag="OQM47_004176"
     CDS             complement(<1..541)
                     /gene="uidR"
                     /locus_tag="OQM47_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1482080.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREAS"
     gene            complement(770..>927)
                     /locus_tag="OQM47_004177"
     CDS             complement(770..>927)
                     /locus_tag="OQM47_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1482081.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
BASE COUNT          220 a          231 c          223 g          253 t
ORIGIN      
        1 acgacgcttc acgcgccagg tcttccgctt cgatatcggc cagtacaccg taggtcagat
       61 ccagcagtaa acgtgaagtc attgccgtgt tgatgtctgg gcttatctcg cctttttgct
      121 gggcatcacg catccgctgg gcaacaaatt cagtgatcgt catatgctta tttttaagca
      181 tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcgaccacc agcgcccgtt
      241 gcccaaaggc ttcatgggtg agagagacaa tagactcgac catatagtca acaaaatgaa
      301 tcccttcaat cggttcccgg aaacgagcca gcgccctctc ctggtcctgt aagataatcg
      361 cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccagga ctaatggcgc
      421 aagatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc
      481 tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca
      541 ttaaaccatt atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc
      601 attgctgttg aatagtgcga aggtacactc tattcgtata cttttcaccc agcaattggt
      661 taaccaatag ccgtgatttg gctgccagtt gtttagcaga gacgttgatg aattgcacac
      721 gctatcccgg taaacaacac atacgaccgt ttaccggttc gttagtgtat taattgagct
      781 cctgtacccc accaccggag acggtgagaa tttgtccgct tacccagctg gctgcaggcg
      841 agcaaaggaa cagcgccgcg ttagcaatat cttgcggttg gcccagacgt ctgatcggcg
      901 tgtgctgtaa cattttttgt tcaattt
//
LOCUS       DAJVPT010000302          927 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVPT010000302 DAJVPT010000000
VERSION     DAJVPT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            45..800
                     /locus_tag="OQM47_004178"
     CDS             45..800
                     /locus_tag="OQM47_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCS1482082.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          256 a          213 c          215 g          243 t
ORIGIN      
        1 cttttgtgct ctctattgtt tgttccgcat tcggcatttt gtaaatggaa cgaggcactg
       61 cttctggcgg tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg
      121 aaaactatac ggcgttaagt gaacgagcca gcgaatattt attggctgtg atccgtagca
      181 aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc attactgacg tatcattatc
      241 tggtagaaaa aatccaccag cagcaggttg atatcagcca gctcaccttc gtgaagctcg
      301 acgaatgggt ggatctgcca ttaacgatgc ctggcacctg cgaaactttc ctacaacagc
      361 atatcgtgca gccgctgggg ctacgtgaag accagctcat cagctttcgc tccgaagaga
      421 taaatgagac agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc ggtctggatt
      481 tatgcgttct cggattgggg aaaaacggtc atcttgggct gaacgaaccg ggagaaagcc
      541 tacaaccggc ctgccatatc agtcaacttg atgccagaac acagcaacat gagatgttaa
      601 aaaccacggg tcgccccgtg actcgtggga tcaccttagg cctgaaggat attctcaatg
      661 cccgcgaggt tttgttattg gtgactggcg aaggaaagca ggatgcgaca gaacgttttc
      721 tcacggctaa agtctccacc gctatcccgg cttcattttt atggctgcac agtaacttta
      781 tttgtttaat taatacctaa ccagatgaaa aatttgctca aaatttttaa attaacttat
      841 tcaacagtca cgcattatat taaataacat ttgactgggt tgaacataac gccaatagca
      901 aaaggagtta tgttcaacaa tttaatt
//
LOCUS       DAJVPT010000303          922 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVPT010000303 DAJVPT010000000
VERSION     DAJVPT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..913
                     /locus_tag="OQM47_004179"
     CDS             <1..913
                     /locus_tag="OQM47_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1482083.1"
                     /translation="EAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALV
                     LMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQLQF
                     SEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLA
                     AAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
BASE COUNT          174 a          211 c          281 g          256 t
ORIGIN      
        1 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cacaaaaaga
       61 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
      121 tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg ttatcatcgg
      181 cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
      241 tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc gtccacagct
      301 gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
      361 tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg gttatttgca
      421 agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc ctttcctcgc
      481 ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga aaaaaggcta
      541 cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt cggcattagg
      601 cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta tctcaatggc
      661 gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg tgatggtgtc
      721 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
      781 ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta atctggcgct
      841 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
      901 tcgcattgag taatcgttac ta
//
LOCUS       DAJVPT010000304          919 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVPT010000304 DAJVPT010000000
VERSION     DAJVPT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            290..856
                     /locus_tag="OQM47_004180"
     CDS             290..856
                     /locus_tag="OQM47_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1482084.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT          305 a          191 c          182 g          241 t
ORIGIN      
        1 ttgagatagt gtcttcccct tcatacgaac agcagccttt atctgctctg cgtgccagtc
       61 ttggtttttt gctgacattc tgcgatcctt actctcttaa cgttttcggt aactgatacc
      121 ggctaccgct atcgggaaaa acgatagcgc agaaaaatgc tcaattcaaa agctatgcgc
      181 atttttattt taaccagtgc aaatcaaaga attctgtaaa attctttata taacagagag
      241 attgggcatg acaggtgaaa aagaaagcaa aaatgcagag cgcattttta tgcccccgga
      301 aataatgcgt ttcaaggaac gccttgaaga ggcaatgaat ggagaatcaa gccgggcgtt
      361 tgcagctaag tgcggacttt ctgatggggt tatcagaaac tacctgtctg gcaagacata
      421 cccgtcttta gatcgattag cacaaatagc ctacgcaaca gggcgtccaa tagagtggtt
      481 tatccaaagt cagagcatgc caatggttga gcataaaaat aatcaagaga ataaaaattc
      541 tcaacctgac cgcagcacat tgaaaactaa actgatcgca attgtcgaat tgttagagga
      601 caaggaactg cattctgcga ttgagttgtt cagaaagaaa ggatttgaag ctcttatgcc
      661 tgagattttt tcttccgatg attccatcca ggctgatttg caaggtattt cgcagcaaac
      721 cttacaaaca gccaaaatgc ttgaatcatt accaacagat actcgtaaaa agattttgtc
      781 aaagtatggt atttatgaac aggaaggtct tgtagctcct tcacaagaac cacaagatgt
      841 aaaaaaagcc gtataacaac catgaaccag ctaaaacata cttcacctca cataatcaca
      901 catgtaacat acctaaacg
//
LOCUS       DAJVPT010000305          900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVPT010000305 DAJVPT010000000
VERSION     DAJVPT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..900
                     /locus_tag="OQM47_004181"
                     /pseudo
     CDS             1..900
                     /locus_tag="OQM47_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          306 a          179 c          177 g          238 t
ORIGIN      
        1 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
       61 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
      121 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
      181 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
      241 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
      301 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
      361 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
      421 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
      481 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
      541 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
      601 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
      661 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
      721 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
      781 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
      841 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
//
LOCUS       DAJVPT010000306          900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVPT010000306 DAJVPT010000000
VERSION     DAJVPT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..489
                     /locus_tag="OQM47_004182"
     CDS             <1..489
                     /locus_tag="OQM47_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1482085.1"
                     /translation="SHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTR
                     WDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELGFH
                     YLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPRLP
                     DD"
     gene            492..>900
                     /gene="capU"
                     /locus_tag="OQM47_004183"
     CDS             492..>900
                     /gene="capU"
                     /locus_tag="OQM47_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1482086.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGT"
BASE COUNT          294 a          153 c          194 g          259 t
ORIGIN      
        1 tctcatcgcg attgcgaaca tcaaatagca accgggaatg ctgataatgt tatgcttcgc
       61 tggtctgaag tgaacgaaat gttgcaatct ggtctggtcg aatttcatgt tcatacacat
      121 actcatacac gttgggataa aaagttcact tcacgagaag aacagtgtaa acatcttcgg
      181 caggacttat tgagcgggag agaatattta aaaaaaatga caggaaaatg cagtaaacat
      241 ttgtgttggc cggaaggtta ttataacaag gattatattc agatcgctga agaacttgga
      301 ttccattatt tatatacaac agaaagaaga atgaatgctc ctgctaaagg aactacacgg
      361 ataggaagaa taagcaccaa ggaacgggaa aattgcgtat ggctgaaaag acgacttttt
      421 tattatacaa caccgttttt ttcatcgttg ctggcccttc ataaggggcc aagattaccg
      481 gatgactaat aatgaatata ctatttacgg aatcatcacc gaatataggt ggtcaggaat
      541 tacaggctgt tgctcaaatg aaggccctga agaaaatggg gcattcagtt ctgcttgtct
      601 gcagggagaa cagcaaaatt gcttttgaag ccagtaagtt tggaattgat atcacattcg
      661 cgttatttcg aaacagtctt cacattccta ctgtatggag attactcgga atagttcata
      721 gttttcagcc cgatgcaatc gtttgtcaca gtggacatga tagtaatatt gttggtttgg
      781 tacggttttt tacctggaaa catccattca gaattatcag gcaaaagaca tatttgacac
      841 gaaaaacaaa atttttttca ataaatcatt tttgcgatga ggtgattgtt cccggaacaa
//
LOCUS       DAJVPT010000307          894 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVPT010000307 DAJVPT010000000
VERSION     DAJVPT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            138..422
                     /locus_tag="OQM47_004184"
     CDS             138..422
                     /locus_tag="OQM47_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1482087.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            410..>894
                     /locus_tag="OQM47_004185"
     CDS             410..>894
                     /locus_tag="OQM47_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1482088.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          247 a          186 c          204 g          257 t
ORIGIN      
        1 gcccctgcgc cacccgatat cccctttgag tgcggttacg tccacagagg taaatgccgc
       61 tgacgcggca tctgtaatct gttactttgt gtatatcatt tgctatatat tgtcaatatg
      121 ctattgttgt aaagcaaatg atatacggag gctttatgaa atcaggtgtt cagctcaatc
      181 tcagagcaag agaatcacag cggatactca ttgatgctgc tgcagaaatt cttcataagt
      241 cgcgtacaga tttcattctg gaaatggcct gtaaagctgc tgaagatgtt attctcgacc
      301 ggcgggtgtt taattttaat gacaggcaat atgaagagtt cattgaaatg cttgatgctc
      361 cggttgcaga tgatcctgcc atcgaaaaat tgctggcaag gaaacctcaa tgggatgtgt
      421 aactgcacca gaacctttat cctcatttca tcaggtcgct gaatttgtca gcagtgaagc
      481 tgtacttgat gactggttaa agcagaagga gctaaaaaat caggcaatcg gagccacccg
      541 gacttttgtt gtatgcagaa aaggtacgca acaaatagtc ggtttttact cactggccac
      601 tggtagtgtc aatcatacag aagctacagg taaccttcgg cgcaatatgc cagaccccat
      661 tcctgtaatt atacttgcac gcctggctgt agatgtgtca tttcgaggaa aagggcttgg
      721 cgcggattta cttcatgatg cagttcgtcg ttgttacagg gttgccgaaa atatcggtgt
      781 gcgtgcaatt atggttcatg cgcttacgga aaacgcaaaa cagttttata ttcatcatgg
      841 ttttaagcca tcaaaaactc aggtgcaaac attgtttctt aaactccctc aata
//
LOCUS       DAJVPT010000308          893 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVPT010000308 DAJVPT010000000
VERSION     DAJVPT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            664..>893
                     /locus_tag="OQM47_004186"
     CDS             664..>893
                     /locus_tag="OQM47_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482089.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHY"
BASE COUNT          230 a          222 c          270 g          171 t
ORIGIN      
        1 ggagttcagg gcgggcatgg atgcttaaat gaaccgcgag tctgtctgga atattgaacc
       61 ggtaactcac gatgagaaac ccaacaatcc caccgggtgt gacggtggag aacctgagcg
      121 gcagtgacct gcggcatgcc cgcagggtga tgtaacccgc tgacaacggg gattgaggcg
      181 agatcactaa gccgagatga tcctcaaggt taagtactga aaggctgaag aacatgaacc
      241 cgttaatccg cctctgtggg ttgaaaacgt caccacggcc tacgtgatct gacaggccgt
      301 gcaggaggaa ctggcagtga tacgtaagca ctgccggtcg aaggtgtttt gacatgtatg
      361 cgaaacaccg gggcagcagc gtctatcaca cttgcgttgc tgacttctgc caacttgcgg
      421 caagcaagga taaagagtgc gacgggcagc ctcctcagta tgcctgagtc caggcaggta
      481 aaccggggaa ggtcagcgac ggatgttaag ggggcatggc tccgatgacg cgctggctgg
      541 cggagcttcc gtagtagtcc gcgatgggga aagcccatta catggcgaag ggaagcagtt
      601 tgaatgtgtt tgcgacgtga attaactgac ctaacgaggt gaagaccttt gataatcagc
      661 gaaatgcaac gcaagcttgc cacatgggca gccaccgatc cgtccctacg gattcaacgg
      721 ctgctgcgtc tgataacaca accagaatgg ctggctgaag cggcgcggat cacgctttca
      781 tcaaaggggg cccatacccc cggcgttgat ggcgtgaaca aaacaatgct acaggccaga
      841 ctggctgttg agctgcaaat cctcagggat gaattactct caggccacta cca
//
LOCUS       DAJVPT010000309          891 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVPT010000309 DAJVPT010000000
VERSION     DAJVPT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..553
                     /locus_tag="OQM47_004187"
                     /pseudo
     CDS             1..553
                     /locus_tag="OQM47_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            555..>891
                     /locus_tag="OQM47_004188"
     CDS             555..>891
                     /locus_tag="OQM47_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1482090.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSD"
BASE COUNT          216 a          215 c          235 g          225 t
ORIGIN      
        1 atgtttacgt cacccataat ccgaaaaaaa cagaacaaaa agccggagtc agtatccatt
       61 aacacccggt tcgctttatg ttggcgtgta cggcggcagc ccggcagata tcgcggcggc
      121 catctggcgt aaggctccgc cgggtattga tatgaatggc aacacaacgt tcactgttgc
      181 agataaggag tacgatccgc cgtatcctga atacgtgatc agctggcaga cactcaaacc
      241 cgtcagtctg catgtcagtg tgacgctgaa aaaaagtgac tacctgcctt cagatattac
      301 ccgacaggta cagcaatccg tgctggacgc atttaatggt acagatggtg gtctgcgggc
      361 aagagttgcc tctgttgtct ctgccgggcg ctactatgcc ggcatttaca aaaccgatcc
      421 ggaacatatt gatattctgg gccttactgt gagccgtgat ggttcgtcat ggacaacggc
      481 tgtcactttc gggatagatg agattccggt tctggatgtg tcggatatca gtgtgaaact
      541 ccaggaggca taacgtgcag aatgtggctg ccactgtgct tgcgcagtat gctgccagcc
      601 cccgactcaa tgccctcatt aacagcttta acgcagcgct ttcccccgac agttttatca
      661 gtgattttta tggtcttatc tggaacatcg ataccgcaga aaagtatggt cttgatgtct
      721 ggggaaagat tgtgggtgtc agtcgctggc tgacggtaaa ggacgatttt aattacctgg
      781 gcttcagcga gtccaggatg gacaccccgg taatggatga tccctgtccg tttaatcagg
      841 caccgtttta caacggaaaa tcggataccc ggactgttga cctgtctgat g
//
LOCUS       DAJVPT010000310          880 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVPT010000310 DAJVPT010000000
VERSION     DAJVPT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..316)
                     /gene="psiF"
                     /locus_tag="OQM47_004189"
     CDS             complement(<1..316)
                     /gene="psiF"
                     /locus_tag="OQM47_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1482091.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKA"
     gene            complement(435..>880)
                     /locus_tag="OQM47_004190"
     CDS             complement(435..>880)
                     /locus_tag="OQM47_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1482092.1"
                     /translation="TDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLD
                     EAVQRALEFAKKEGNTLVIVTADHAHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGN
                     SEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
BASE COUNT          192 a          238 c          204 g          246 t
ORIGIN      
        1 cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc agagcttgtt
       61 gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa cttttttcac
      121 caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt
      181 tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta
      241 aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc aaggtaacca
      301 gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg tttgcgcccg
      361 tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac
      421 tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa gagatcggtc
      481 tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat acgcaactga
      541 ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac catcactgcg
      601 ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg cgcaacaacc
      661 tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc ctttttagcg
      721 aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa
      781 gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct
      841 ttctcatttt tactcaacaa ttcaatggct ttgtcggtca
//
LOCUS       DAJVPT010000311          868 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVPT010000311 DAJVPT010000000
VERSION     DAJVPT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..272)
                     /locus_tag="OQM47_004191"
     CDS             complement(<1..272)
                     /locus_tag="OQM47_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1482093.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVA"
     gene            522..>868
                     /locus_tag="OQM47_004192"
     CDS             522..>868
                     /locus_tag="OQM47_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482094.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPV"
BASE COUNT          196 a          218 c          201 g          253 t
ORIGIN      
        1 gcgacctgcg aagtcgaatg cccctgaacc gcgcggatgc acctaccatc actggaataa
       61 ctaaaacgct ttttatcgct ggttgcgacc acggtatcca gcaatgcacg ggttaatcgc
      121 ttgcccgctt tttgggcaca aagtatgagc ttattgatat ccgcccatcc ttcacggtcc
      181 agtacgatac caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta
      241 tccgccagct ctttttcgtt gtactttgcc atcgcgtttt cgtccctgga ttttctgttt
      301 ttatttacta atgtcattgt cactgggttt atgaattttc tgcaactcaa tcctgcgcag
      361 aaccttttcc acacagcgta atttttcccc tatgcaaaaa acgcatagcc agaaacatac
      421 ttgcattttc cccataccca catggctgat aaggtttttc agttccagga attttcctgg
      481 ctcaagcaac acacaaacac aaaaaaacaa tggggattgt tatgacacaa caaggggatg
      541 ctgtagccgg ggagctggcg acagaaaaag tgggcattaa agggtatctg gctttttttc
      601 tcaccataat cttttttccc ggtgtttttt ccggaacaga cagctggtgg cgcgtttttg
      661 atttttccgt tctgaacgga tcgtttgggc aactgcctgg tgcgaatgga gccaccacct
      721 catttcgcgg ggtgggtggc gcgggggcta aagatggctt tctctttgcg ctggagctgg
      781 caccgtcagt cattctctct ctggggatta tttcaattac cgatggtctt ggcggattac
      841 gcgccgcgca gcagttaatg acacctgt
//
LOCUS       DAJVPT010000312          866 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVPT010000312 DAJVPT010000000
VERSION     DAJVPT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>866
                     /locus_tag="OQM47_004193"
     CDS             <1..>866
                     /locus_tag="OQM47_004193"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1482095.1"
                     /translation="ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTY
                     TGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGED
                     PGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASL
                     KYLSDHPKLQGIA"
BASE COUNT          177 a          245 c          246 g          198 t
ORIGIN      
        1 gcccgctact gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg
       61 gacccgaaac gcatgattga agcctgtgac gaaaacacca tcggcgtggt gccgactttc
      121 ggcgtgacct acaccggtaa ctatgagttc ccacaaccgc tgcacgatgc gctggataaa
      181 ttccaggccg acaccggtat cgacatcgac atgcacatcg acgctgccag cggtggcttc
      241 ctggcaccgt tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg
      301 atcagtgctt caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg
      361 cgtgacgaag aagcgctgcc gcaggaactg gtgttcaacg ttgactacct gggtggtcaa
      421 attggtactt ttgccatcaa cttctcccgc ccggcgggtc aggtaattgc acagtactat
      481 gaattcctgc gcctcggtcg tgaaggctat accaaagtac agaacgcctc ttaccaggtt
      541 gccgcttatc tggcggatga aatcgccaaa caggggccgt atgagttcat ctgtacgggt
      601 cgcccggacg aaggcatccc ggcggtttgc ttcaaactga aagatggtga agatccggga
      661 tacaccctgt acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc
      721 actctcggcg gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc
      781 gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc
      841 gatcacccga aactgcaggg tattgc
//
LOCUS       DAJVPT010000313          865 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVPT010000313 DAJVPT010000000
VERSION     DAJVPT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            60..>865
                     /locus_tag="OQM47_004194"
     CDS             60..>865
                     /locus_tag="OQM47_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCS1482096.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREA"
BASE COUNT          219 a          173 c          268 g          205 t
ORIGIN      
        1 ggctggttaa gtatttcgtt tgtcgatttc tggtttaaaa atctcaatct gagggaatca
       61 tgaggccaag tgaacttagc gacctgcttt gggcgcaggt tgacagggtg gctccgcacc
      121 tgttgccaaa cggcaagata gaggggcatg agtgggttgc cggtaacgtc aacggtgaca
      181 agggaaacag ccttaaggtc aaccttatcg gcaagaaaaa atgggctgat ttcgctgagg
      241 gagacggcgg tgacatgctt gatttgtgga tggcatgtcg tggaattaac ctgcatcagg
      301 ctatgcagga agcgaaagca tttctcggta tcaaggatga cgatcaccat ttcgatgcca
      361 gacgtgagaa gaaattctcc agacctgatc gtaagaaaat cgcccgctac gttaccagaa
      421 cagaatccca tcttgagtac ctgcaatcgc gtggcatatc gccagaagtc gtaaagcgct
      481 acgaggttgt cagcggcaag gtgtggaatg gagaacgaga actggatgct ctggtgcttc
      541 cgtacaaacg cgatggtgag ttgttgcagg tcaagcgaat cagcactgag cgcccggacg
      601 ggaagaaagt cattatggca gaaggtgatt gcgaaccttg cctgttcgga tggcaggctc
      661 tggacgctgg cgtgagggtg gttgtgcttt gcgaaggcga aattgattgt atgagctatg
      721 cgcaatacgg catctcggcg ttatccgtgc cgtttggtgg cgggaaaggc gctaagcaac
      781 agtggattga gtttgagtat cacaacctcg acaggtttga ggaaatattc atctcgatgg
      841 acgttgatga tgttggtcgt gaagc
//
LOCUS       DAJVPT010000314          860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVPT010000314 DAJVPT010000000
VERSION     DAJVPT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>860
                     /gene="proB"
                     /locus_tag="OQM47_004195"
     CDS             <1..>860
                     /gene="proB"
                     /locus_tag="OQM47_004195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1482097.1"
                     /translation="IASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRE
                     RFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTD
                     QKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAG
                     IDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATA
                     AILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQ
                     EIDAILGYEY"
BASE COUNT          196 a          218 c          252 g          194 t
ORIGIN      
        1 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
       61 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
      121 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa
      181 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
      241 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
      301 gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg cagaactgat
      361 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
      421 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
      481 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
      541 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
      601 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
      661 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
      721 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
      781 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
      841 aatactggga tatgaatacg
//
LOCUS       DAJVPT010000315          860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVPT010000315 DAJVPT010000000
VERSION     DAJVPT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            178..>860
                     /locus_tag="OQM47_004196"
     CDS             178..>860
                     /locus_tag="OQM47_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1482098.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLG"
BASE COUNT          216 a          204 c          221 g          219 t
ORIGIN      
        1 tttcgcacca ttgcggggcg ctgtttttat tatttcctgt ctacactcag cttattgcga
       61 cgcgaaatcg tgcgcaaaag ctggcactac ttttgcttat aagaggatgg ccacagacag
      121 gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg aagcattatg
      181 aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag
      241 gcgcatgctg gcgcgacgct tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc
      301 agtgatggat tacctaggtt ctcttatgcc gatgcagacg gtaagttttc aggtattgat
      361 gttgatgttt gtcgtggtgt tgccgctgct gtatttggtg acgacacgaa agtgaaatat
      421 accccactca cagcaaaaga acgcttcacc gctttacagt caggggaagt ggatttgctc
      481 tcccgtaata cgacctggac ttcatctcgc gatgccggga tgggaatggc atttaccggc
      541 gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct aaaaagcgcg
      601 aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga actcaacgtc
      661 gccgactatt tcaaggcaaa caatatgaag tacacaccag tgactttcga tcgctctgac
      721 gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcttcgga tcagtcgcaa
      781 ctgtatgcct tgcgcatcaa attaagcaac ccggctgaat ggattgtctt accggaggtt
      841 atctctaaag aaccgcttgg
//
LOCUS       DAJVPT010000316          852 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVPT010000316 DAJVPT010000000
VERSION     DAJVPT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..392
                     /locus_tag="OQM47_004197"
                     /pseudo
     CDS             1..392
                     /locus_tag="OQM47_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          258 a          182 c          171 g          241 t
ORIGIN      
        1 ttcaggagga aatggcggaa acactgggtg agcaatatga cccggtactg ccatcttccc
       61 tgcgccagtc ttcagcccgt aaaccgttac cagcctcact tccccgtgcc ccccgggtca
      121 tccggccgga agaggaatgc tgtccggcct gtggtggtga actcagtcca ctgggatgtg
      181 atgtgtcaga gcagctggag cttatcagca gcgcctttaa ggttatcgaa aaacaacgcc
      241 ctaaactggc ctgtcgccgg tgcgaccata tcgtgcaggc accggtgcct tcaaaaccca
      301 ttgcacgcag ttatgccgga gcggggcttc tggcccatgt tgttaccgga aagtatgcag
      361 accatctgcc attataccgc caaagtgatc tactattcca cacagctatt tagctctctt
      421 tttgttgagc tcccatgatt ctttcattta ttgttactaa ttattttatg ttatcatgat
      481 gttagaacac gctctgctct gtgtctctat tttatctatc atattctgag gattaactac
      541 ataacaaggc gcaagatata tatacaaaaa caaataatac cgttgcaata attcctttta
      601 gtaaaggagg tgtgattaat ttcaggggat ttcagagtaa aaattgtaca acatattaaa
      661 atatgttcat ttcgttataa tacacacaag gaaatattaa gttagataat atattctaaa
      721 taaccataga taaaaacgca cataatgggg aatattttat gaaaatacct gaagcagtaa
      781 atcatattaa tgttcagaat aatattgatc ttgttgatgg aaaaacgaat ccgaataaag
      841 caacaaaagc at
//
LOCUS       DAJVPT010000317          826 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVPT010000317 DAJVPT010000000
VERSION     DAJVPT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>826
                     /locus_tag="OQM47_004198"
     CDS             <1..>826
                     /locus_tag="OQM47_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCS1482099.1"
                     /translation="KYFYVWLDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYH
                     FIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTWLNH
                     FDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGV
                     LASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAK
                     QEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLGH"
BASE COUNT          197 a          215 c          221 g          193 t
ORIGIN      
        1 aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt caagaatctg
       61 tgcgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga ctccaccgcc
      121 gagctgtacc acttcatcgg taaagatatc gtttacttcc acagcctgtt ctggcctgcc
      181 atgctggaag gcagcaactt ccgcaagccg accaacctgt ttgttcatgg ctatgtgacg
      241 gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag cacctggctg
      301 aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc ttcgcgcatt
      361 gatgatatcg atctcaacct ggaagatttc gttcagcgtg tgaatgccga tatcgtgaac
      421 aaagtggtta acctggcctc ccgtaatgcg ggctttatca acaagcgttt tgacggtgtg
      481 ctggcaagcg agctggctga ccctgagctg tacaaaacct ttaccgatgc cgctgaagtg
      541 attggtgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat catggcactg
      601 gctgacctgg ctaaccgcta tgtcgacgag caggctccgt gggtagtcgc aaaacaggaa
      661 ggtcgcgatg ctgacctgca ggcgatttgc tcaatgggca tcaacctgtt ccgcgtgctg
      721 atgacttacc tgaagcctgt actgccgaaa ctgaccgagc gtgcagaagc cttcctcaat
      781 acggaactga cctgggatgg tatccagcaa ccgctgctgg gccaca
//
LOCUS       DAJVPT010000318          821 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVPT010000318 DAJVPT010000000
VERSION     DAJVPT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>821
                     /gene="ydfJ"
                     /locus_tag="OQM47_004199"
     CDS             <1..>821
                     /gene="ydfJ"
                     /locus_tag="OQM47_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1482100.1"
                     /translation="AFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVR"
BASE COUNT          193 a          176 c          208 g          244 t
ORIGIN      
        1 gggcctttat gttcttcatt ctcagtaaag aggaactgct ggcgtgggga tggcgtatac
       61 cgttcctggc gagcgttgtc gtgatggtct ttgctatctg gttgcgtatg aatctgaaag
      121 aaagcccggt ctttgagaag gttaacgaca gcaaccaacc gacagcaaaa cctgcacctg
      181 ctggtagcat gttccagagc aaatccttct ggctggcaac agggctgcgt tttggtcagg
      241 cgggtaactc cggtttaatt cagactttcc ttgcaggcta tttagtacag acgttattgt
      301 ttaacaaagc aattccaaca gatgcattga tgatcagttc gattctcggc tttatgacca
      361 ttccgttcct tggttggtta tccgataaaa ttggtcgccg gatcccgtat attattatga
      421 atacctctgc gattgtgctg gcgtggccaa tgctttctat catcgtagat aaaagctatg
      481 ccccgagcac cattatggtt gcactgattg tgattcataa ctgtgcggtg ctgggattat
      541 ttgctctgga aaacattacc atggcagaaa tgttcggctg taaaaaccgc ttcacccgga
      601 tggctatttc aaaagaaatt ggtggtctta tcgcttccgg ttttggtcct atcctggcgg
      661 gtattttctg caccatgacg gaatcctggt atccgatcgc cattatgatc atggcatatt
      721 cagtgattgg tttaatctct gcgctgaaaa tgccagaggt gaaagaccgt gatttaagtg
      781 cgctggaaga cgctgcggaa gatcaaccgc gtgttgtaag a
//
LOCUS       DAJVPT010000319          820 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVPT010000319 DAJVPT010000000
VERSION     DAJVPT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(74..739)
                     /locus_tag="OQM47_004200"
     CDS             complement(74..739)
                     /locus_tag="OQM47_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1482101.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          215 a          206 c          183 g          216 t
ORIGIN      
        1 atagcctcaa gcacccgcag tcattctact tacctcgcca atatgaaatc aatcagaaag
       61 gtgccccata aaatcactcc ttctcttctt taccgtagtg gagttgacca attttgataa
      121 gagggcgtcc ctgagatttg cggtgtagat tggtatcgcg cagagaatac acacagccac
      181 aatattcctg ctgatagaat ttttcgcgct tgctgatttc aatcatacgg gacgagccgc
      241 cctgcttgcg ccagttataa tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc
      301 gcccacagtc gttaacctgc tgcatatttt tccagcgtga aatgcccagt gaactgctga
      361 tcacactgaa accattttca gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac
      421 acatggtgca acggatccct cgttcgggct cccattccat tcctttggct cgttcaaacc
      481 agttgtctgt gtcgtaatca gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa
      541 tattctcatc cttacgaatt aaatactctt tctgaggatg aatgttcggg ttgtagaaaa
      601 agatggtgta gtcgatttcc gaggcctgaa gcgcctccat cacctcaccg gaacatggag
      661 cacagcaaga gtgcagtagt agtttgtttg ccccgtttgg gagctccaat ttgggccgtt
      721 tgaaatcagc aactgtcata aatatgttta ttgaggtcat aaaaatatca aagagtgtag
      781 cattagaacg gggctatcgg aaacagatgt gtgactgctc
//
LOCUS       DAJVPT010000320          816 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVPT010000320 DAJVPT010000000
VERSION     DAJVPT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..407
                     /locus_tag="OQM47_004201"
     CDS             <1..407
                     /locus_tag="OQM47_004201"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="RNase BN; member of metallo-beta-lactamase family;
                     the purified enzyme from Escherichia coli forms dimeric
                     zinc phosphodiesterase; in Bacillus subtilis this protein
                     is a 3'-tRNA processing endoribonuclease and is essential
                     while in Escherichia coli it is not; associates with two
                     zinc ions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCS1482102.1"
                     /translation="GPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGP
                     CDAALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSS
                     RYDDKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            598..786
                     /locus_tag="OQM47_004202"
                     /pseudo
     CDS             598..786
                     /locus_tag="OQM47_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          235 a          187 c          181 g          213 t
ORIGIN      
        1 ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc
       61 agattaacgg cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg
      121 gcgataccgg cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc
      181 acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta
      241 cacgccaggc tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg
      301 tcagctcgcg ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt
      361 tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat
      421 taaaaacagg gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac
      481 aggcatattg agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat
      541 aagttgcctc ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg
      601 gttacagttg tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca
      661 gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga
      721 gatgaagaaa gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa
      781 aaagctgggt aatgctgcca acttactgat ttagtg
//
LOCUS       DAJVPT010000321          806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVPT010000321 DAJVPT010000000
VERSION     DAJVPT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>806)
                     /gene="narU"
                     /locus_tag="OQM47_004203"
     CDS             complement(<1..>806)
                     /gene="narU"
                     /locus_tag="OQM47_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1482103.1"
                     /translation="KNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVW
                     MLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWPVFSTA
                     ILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALG
                     INGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIA
                     MIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAG"
BASE COUNT          258 a          189 c          193 g          166 t
ORIGIN      
        1 aacccgcaga aaaaccgata aacgaaccga aggtggcaag gtaaagcagg ctcagcagcc
       61 agagatgcag gcgttgtaag acagggagct ggtcggcaat tgaggcgcgt gaactggcga
      121 tatcattcat ccctgaccag gcggcgatca tggcaatcgc cagtagcggt acccaaatcc
      181 atgcggcatt cgccagcgac atcaccgaac cgtcggcctg cggtacgcca ttgacgccga
      241 gaaaggcaaa tacaggtaca aaaatgacca gcggtgcaac cagctgcatt acacttacac
      301 ctaagtttcc taatccgcca ttaatcccaa gagcgctccc ttgtttggct tttggaaaga
      361 agaaactgat attgcccatg ctcgaagcaa agtttgcacc tgcaaaaccg catagcaaag
      421 cgataacgat aaatatccca aaaggagtat tcggattttg cacggcaatt ccgagccaga
      481 cgcaaggaat aatcaggatt gcagtactaa aaaccggcca tcggcgtcca ccgaatatag
      541 gcaccataaa ggagtaggga acacgcaata atgcgccaga aacggagggt aatgcggtta
      601 ataaaaagag ttgatcggta gtaaaattaa aaccgatttt attgagatta acggtaactg
      661 cgctaaatag catccagaca cagaaggcaa gaagtagaca actgactgat atccagaggt
      721 ttcttcgagc aatatgtttt cctttatttt cccagaaggc cggattttct ggtttccagt
      781 cgcgtaaaag ataacgacta tttttc
//
LOCUS       DAJVPT010000322          795 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVPT010000322 DAJVPT010000000
VERSION     DAJVPT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            422..498
                     /locus_tag="OQM47_004204"
     tRNA            422..498
                     /locus_tag="OQM47_004204"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:456..458,aa:Met,seq:cat)
     gene            507..591
                     /locus_tag="OQM47_004205"
     tRNA            507..591
                     /locus_tag="OQM47_004205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:541..543,aa:Leu,seq:tag)
     gene            615..689
                     /locus_tag="OQM47_004206"
     tRNA            615..689
                     /locus_tag="OQM47_004206"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:647..649,aa:Gln,seq:ttg)
     gene            724..795
                     /locus_tag="OQM47_004207"
     tRNA            724..795
                     /locus_tag="OQM47_004207"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:756..758,aa:Gln,seq:ttg)
BASE COUNT          198 a          193 c          202 g          202 t
ORIGIN      
        1 gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc ggtactgccc
       61 cggagaaatc cggggctgtt ttagttattg cgctgcttgt tgattctgat atgcagtaag
      121 gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg ccagtatttc
      181 atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac gacaaaagaa
      241 tggctgtagg gtggggaaaa acacaaagta aacaataatt gacgaatata gcgccacgct
      301 gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag aggttgacgc
      361 tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc gaaaaaaaga
      421 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
      481 atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac gcaccagatt
      541 taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga
      601 aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
      661 ccctggttcg aatccaggta ccccagccat cttcttcgag taagcggttc accgcccggt
      721 tattggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga
      781 atccaggtac cccag
//
LOCUS       DAJVPT010000323          793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVPT010000323 DAJVPT010000000
VERSION     DAJVPT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            197..448
                     /locus_tag="OQM47_004208"
     CDS             197..448
                     /locus_tag="OQM47_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482104.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            515..793
                     /locus_tag="OQM47_004209"
                     /pseudo
     CDS             515..793
                     /locus_tag="OQM47_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          211 a          190 c          191 g          201 t
ORIGIN      
        1 ccctcgccac ctctgatgtg gcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc
       61 gggtttttgt ttttattttc aacgcatttg aagttctgga tggtgccgga atagaatcaa
      121 aaatacttaa gtagcgcgca gggataaaag ggatggaccc cgaacagggg agtgctactt
      181 atctggaagg attctgatga tgaacatcga agaactgcgt aaaatttttt gtgaagatgg
      241 cctctatgct gtgtgcgttg aaaatggaaa tattgttagt cattaccgca ttgtgtgttt
      301 gcaaaaaaat ggggctgcgt taattaattt tgtggatgcc cgagtgacgg acggatttat
      361 cttgcgcgac ggtgagtttg tcacttcatt acaggcattg aaagagatcg gaataaaagc
      421 tggcttttct gctttttcag aagaataaac tcatctacaa tcttgcgcgg ggctgaactc
      481 ccgctgagta acaccgtgcc accggagaaa accgatggca cgcaacgcaa aatattacaa
      541 tcatgataat tcgaccgttc ttgcccacac gcacgagcgg tattctcacg catttaagtc
      601 agactggtac cagcatccct catgcactga agaacaggcc gaatggctta ttcagtgtta
      661 ccgcaggcgc ggatgcgagg ttaaaaaagc ccttagcctc gactaccgtc actagataat
      721 ctccgtcagg ctcccttact ccgaacagcc accgcgtccg tcccgcacat tccagcaacg
      781 gatctggagg taa
//
LOCUS       DAJVPT010000324          793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVPT010000324 DAJVPT010000000
VERSION     DAJVPT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            109..654
                     /locus_tag="OQM47_004210"
     CDS             109..654
                     /locus_tag="OQM47_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1482105.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            678..>793
                     /locus_tag="OQM47_004211"
     CDS             678..>793
                     /locus_tag="OQM47_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1482106.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVA"
BASE COUNT          231 a          174 c          202 g          186 t
ORIGIN      
        1 gatatcggat accggccgta actcagcgga taacgttgac ggtgtgtatt accgaccact
       61 gcaaaaactg attaatggca cctggtataa cgtagcgagt atttaacaat gttgcattta
      121 aaaaatatta ctgcgggtaa tccgaaaaca gcagaacaat atcagatgac aaaacgatat
      181 tcggtcacct ggcttttttc agaagacgga aaaaactggt atgaagagct gaagaatttc
      241 gccagcgaca caataaaaat agcttacacc ggagatggtc gcgtggtgtg ggtcggtaag
      301 gatgtgacag gcatcgagcc acgcaatgcc agcgttatcg aagttccgga tattaccgct
      361 aaccgacgga ttaccgcgcc gggttactgg ttttaccgca atgatgaatt tgtctttgac
      421 tacagactca aagcggaaga tgagcgtgat gcccttctgg ctcaggtcag tgcccggaca
      481 ggggaatggg aagaagacct gctgctgggg ttaatcagcg acgaagataa agaaaagctg
      541 aaagcctgtc gtatttacgc gaaatcgctg caggcgatgg atttcagcac catcactgat
      601 aaatcctcat acaacgccat tgaatggccc gcctctccgg aaggttcttc ctgatttaat
      661 ttatcgcgag aaaaacaatg tctgtagtga tatcaggtgc gctgactgat ggcgcaggta
      721 tccccatgtc cggataccat attattctga aatcccgggt aaaccccccg gaagtggtga
      781 tgaacactgt tgc
//
LOCUS       DAJVPT010000325          790 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVPT010000325 DAJVPT010000000
VERSION     DAJVPT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            6..224
                     /locus_tag="OQM47_004212"
     CDS             6..224
                     /locus_tag="OQM47_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1482107.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OQM47_004213"
     CDS             226..591
                     /locus_tag="OQM47_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1482108.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..>790
                     /locus_tag="OQM47_004214"
     CDS             588..>790
                     /locus_tag="OQM47_004214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482109.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSL"
BASE COUNT          227 a          149 c          205 g          209 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct
//
LOCUS       DAJVPT010000326          782 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVPT010000326 DAJVPT010000000
VERSION     DAJVPT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(129..611)
                     /gene="sseA"
                     /locus_tag="OQM47_004215"
                     /pseudo
     CDS             complement(129..611)
                     /gene="sseA"
                     /locus_tag="OQM47_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          174 a          225 c          189 g          194 t
ORIGIN      
        1 gagtattgtt ttgctgtgaa gcacctgcgt tgctcatgta acacaagtca acctgctgga
       61 aaggccgcga accagaccag caataaaaaa acgccaaatt cggcggtttt ttattgcctg
      121 taatatactt atttcactgg ctcaaccggt aaatctgccc gcgcgcccca ttcactccat
      181 gcgccgtcgt acagtttcac gtttggcaca tccagcgtcg cgagtgctaa caaaaccacg
      241 gctgccgtta caccagagcc gcagctgacg ataatcggtt tgtcatagct gacgccgcga
      301 ccaaaaaata tcgcatccag ttcatcggtc gtttttagtt cgccttcgcg caccagttcc
      361 gtccacggaa cgttcaatgc accgggaata tgtccgcgac gtaaacctgg gcgaggttca
      421 tcaacttctg cgttaaaacg tgcagccggg cgggcatcaa taatttgcgc cgtattttca
      481 tggcttgcca ataacacatc ggttactttc accactgctt caggattaaa cgcggcgtta
      541 aactctcctt ccggcagctc tactgcacct tcttctaata gcaggtcatc gcgctgccag
      601 cctgcaagtc cccgttactt tattcgtacc ccttataatg gggtgttagc cagccagacc
      661 cggcatgatt actgccccca gtcgtccatg atccgggggg ggatgtcacc gggtctggtg
      721 gggcgctggt aaccgctaat aggggtcagg tcaggcactt ttgccgggac cgtctgtaac
      781 gt
//
LOCUS       DAJVPT010000327          774 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVPT010000327 DAJVPT010000000
VERSION     DAJVPT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..122
                     /locus_tag="OQM47_004216"
     CDS             <1..122
                     /locus_tag="OQM47_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1482110.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="OQM47_004217"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="OQM47_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1482111.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
BASE COUNT          223 a          167 c          191 g          193 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gttt
//
LOCUS       DAJVPT010000328          774 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVPT010000328 DAJVPT010000000
VERSION     DAJVPT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(23..82)
                     /locus_tag="OQM47_004218"
     CDS             complement(23..82)
                     /locus_tag="OQM47_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1482112.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(321..758)
                     /locus_tag="OQM47_004219"
     CDS             complement(321..758)
                     /locus_tag="OQM47_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482113.1"
                     /translation="MQAAAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFK
                     LALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSA
                     ALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          171 a          209 c          179 g          215 t
ORIGIN      
        1 ttctcccccc tctgattggc tgttaataag ctgcgaaact tacgagtaac aacacaatca
       61 gtatgatgac gagcttcatc ataacccttt ccttctgtaa ggccccttcc tcgggagggg
      121 ctttcccgtt tcagcccttt gctgactccc cgattgttga tgagcgccgg acctcgcgca
      181 acgctttccc agaggcagtt ttgccttccc cgagcggtca cgcaaaacgt tgcaacgacc
      241 tgatcatttg cagtttttag cggaacgcag atataaaaaa tccccggctt tttgtgccag
      301 ggacgttcat agctgaaaat ttattttacg ttttcggcga cgggagttgt tgagattaag
      361 ggtgaagtca atgccgccag tgaaccgcct gaaacgccaa tttcattgag caaggagtca
      421 atcagcggag cctgtgtgcg gtaagagaga gcggctgata atgcttgttc cgctaggttt
      481 ccgcccccca cattacccgt gtttgcatca cccgcagcgc tgccactatt cagaccatcg
      541 acctgaataa tcttgatacc gtcaatagat ttcatcggct caacggattt ttctattacc
      601 gcaggtagcg cctgcaacaa ggccagtttg aatttaaggc tggtttgttc atcagaaagt
      661 acgttgatag catcgttcag cgcacgttgt gcttctgctt ccgccagacc ctttttatgt
      721 gtagcttcgg ctaactcaac gatagccgcc gcctgcatct ctgcggcttc tttt
//
LOCUS       DAJVPT010000329          770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVPT010000329 DAJVPT010000000
VERSION     DAJVPT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..252
                     /locus_tag="OQM47_004220"
     CDS             <1..252
                     /locus_tag="OQM47_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1482114.1"
                     /translation="LAKVGSFAGTATTGVGIRIDDAESGNLMPLNAMGNDNTIYQIPA
                     DSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            332..>770
                     /locus_tag="OQM47_004221"
     CDS             332..>770
                     /locus_tag="OQM47_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1482115.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKG"
BASE COUNT          215 a          175 c          176 g          204 t
ORIGIN      
        1 ctggcaaaag tgggctcttt tgctggcaca gcgacaacag gtgtgggtat ccgtattgac
       61 gatgcggaaa gcggaaacct tatgccactg aatgctatgg ggaatgataa cacgatttat
      121 cagatcccag cggactccaa tggtattgtc aatgttgacc tcatcgctta ctacgtatca
      181 accgttgaag catcagaaat caccccaggg gaagcagacg ctgtcgttaa cgtaacgctg
      241 gattaccgtt aatacgttac ggcgttatct gacctttcag ataacgccct tttccttcct
      301 ctttctcgtt gtatcaggtt gaaaaataac tatgtcaaaa cgaacattcg cggtgatatt
      361 aaccttgttg tgtagcttct gtatcggcca ggcgcttgca gggggtatcg ttttacagcg
      421 aacgcgagtg atctatgatg ccagccgcaa agaggctgcg ttacctgtcg caaacaaagg
      481 cgcagaaacg ccttatttac tgcaatcatg ggtagataat atagatggta aaagccgtgc
      541 cccatttatt ataaccccac cgctatttcg tcttgaggct agcgatgact catcactgcg
      601 aattattaaa acagctgata acctgcctga aaataaagag tcgctgttct acattaatgt
      661 tcgtgccatt ccagcaaaga aaaaatcaga tgatgttaat gctaacgagt tgacgctggt
      721 atttaaaacg cgggtcaaaa tgttttatcg ccccgcacac ctgaagggac
//
LOCUS       DAJVPT010000330          769 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVPT010000330 DAJVPT010000000
VERSION     DAJVPT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..769
                     /locus_tag="OQM47_004222"
                     /pseudo
     CDS             1..769
                     /locus_tag="OQM47_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          184 a          193 c          229 g          163 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatc
//
LOCUS       DAJVPT010000331          768 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVPT010000331 DAJVPT010000000
VERSION     DAJVPT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>768
                     /gene="proA"
                     /locus_tag="OQM47_004223"
     CDS             <1..>768
                     /gene="proA"
                     /locus_tag="OQM47_004223"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1482116.1"
                     /translation="GIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVI
                     YEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQDALKSCGLPAGAVQAI
                     DNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAE
                     IAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQMAESGVTLHADAAALT
                     QLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDA"
BASE COUNT          182 a          177 c          237 g          172 t
ORIGIN      
        1 aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat
       61 cgatggcggc gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt
      121 tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct
      181 gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa acctgtcgca ctaacgcggc
      241 aacggtggcg gtgattcagg acgccctgaa atcctgtggc ttaccggcgg gtgccgtgca
      301 ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat
      361 cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac
      421 aatcccggtg atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagtgcaga
      481 gatcgctgaa gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa
      541 tacggttgaa acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag
      601 caaacaaatg gcggaaagcg gcgtgacatt acacgcagat gcagctgcgc tgacacagtt
      661 gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa gagtatgacg atgagtttct
      721 gtcattagat ttgaacgtca aaatcgtcag cgatcttgac gatgccat
//
LOCUS       DAJVPT010000332          759 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVPT010000332 DAJVPT010000000
VERSION     DAJVPT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(134..322)
                     /locus_tag="OQM47_004224"
     CDS             complement(134..322)
                     /locus_tag="OQM47_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482117.1"
                     /translation="MMLLFRGEELAFSSAFSPVTIRKIMKCPSGEEDRRKKDPPAKTL
                     GMNSFRYSDCWRVKFLMN"
     gene            complement(656..730)
                     /locus_tag="OQM47_004225"
     tRNA            complement(656..730)
                     /locus_tag="OQM47_004225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(695..697),aa:Gly,seq:tcc)
BASE COUNT          235 a          183 c          158 g          183 t
ORIGIN      
        1 gctatcggaa agaaaaaagg ccatcagcaa aactctgatg acctgaatca cctttaccaa
       61 tattgtatga aaaaacacgc acgactaatt gacaataatt ttcatttcca ttaaaaaaga
      121 tcgcgtagca ttcttaattc atgaggaact ttacccgcca gcaatctgag tagcgaaagc
      181 tgttcatccc caatgttttt gctggcgggt ccttttttct acggtcctcc tccccggagg
      241 ggcatttcat tatttttcta atcgttactg gtgagaaggc ggaagaaaaa gccagttctt
      301 cgcccctaaa taacagcatg attccagtcc atgcaaaatc tgatttccag ctcacacata
      361 ccgagcgctg tacaaaaaac cgccagccaa ggcagttaag agtgtgttcc cggggtttgc
      421 ttagaatatt ttgtaagttg tccgaagaga tatttacaac accagaatga tgaatcatca
      481 gtcccctgcc agaggttcac tacgtaacct gaccgacaat gcaccattct ggtgctgaaa
      541 aaagagcact ggaactgcaa caaaacaaca tcacagaaca ggagatggta aggagtcaga
      601 cattcccaca caatattgtg tcagtgcaaa taacaacctt cgtctcaaat ctcgctggag
      661 cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata
      721 cgatgcccgc atatggtgcc gactaccgga atcgaactg
//
LOCUS       DAJVPT010000333          755 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVPT010000333 DAJVPT010000000
VERSION     DAJVPT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..188
                     /locus_tag="OQM47_004226"
     CDS             <1..188
                     /locus_tag="OQM47_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1482118.1"
                     /translation="IVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTSYFISVFKAFY
                     SLTPLNYLAKQRQKVMW"
     gene            complement(234..310)
                     /locus_tag="OQM47_004227"
     tRNA            complement(234..310)
                     /locus_tag="OQM47_004227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Arg,seq:tct)
     gene            553..>755
                     /locus_tag="OQM47_004228"
     CDS             553..>755
                     /locus_tag="OQM47_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1482119.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT          216 a          166 c          171 g          202 t
ORIGIN      
        1 agattgtcac agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa
       61 atatcactca ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt
      121 ttaaggcgtt ttacagcctg acaccgttga attatctcgc caaacagcga caaaaagtga
      181 tgtggtgaag ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg
      241 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
      301 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
      361 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
      421 atttatttcg ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc
      481 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
      541 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
      601 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
      661 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      721 atagacttgc ccggaacaga cggttttacc ttcct
//
LOCUS       DAJVPT010000334          755 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVPT010000334 DAJVPT010000000
VERSION     DAJVPT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..221)
                     /locus_tag="OQM47_004229"
     CDS             complement(<1..221)
                     /locus_tag="OQM47_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482120.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSR"
     gene            complement(354..659)
                     /locus_tag="OQM47_004230"
                     /pseudo
     CDS             complement(354..659)
                     /locus_tag="OQM47_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          174 a          192 c          183 g          206 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaa
//
LOCUS       DAJVPT010000335          745 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVPT010000335 DAJVPT010000000
VERSION     DAJVPT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..185)
                     /locus_tag="OQM47_004231"
     CDS             complement(<1..185)
                     /locus_tag="OQM47_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HCS1482121.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRG"
     gene            371..745
                     /locus_tag="OQM47_004232"
                     /pseudo
     CDS             371..745
                     /locus_tag="OQM47_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          254 a          165 c          146 g          180 t
ORIGIN      
        1 ccccgataaa aaaactccag ttcatctgat gacagtgttt tccagttgtt ttcagccaac
       61 catttcattt gtttccggaa tgtctcggga gaaagtgtaa ccagtcctgg gcaatggctg
      121 acatgatgat acattaatac cggaacatga tttgctttaa acaaaatgcc ccctccgttt
      181 aaaatttaaa gtttctggca cccagtaagt agagataaaa ggaaagccta ataaaggtgg
      241 tgaactttat atactactaa atcaaacatc aacaatttat aaacaataat atattatcat
      301 atcttttaaa aacggctcac taagaacctt gaggtggcac actaaattta gctacctgaa
      361 cagaggtgat atgctcacct cagaacaaca caggtgccat aatgaaaaaa gaaatttcag
      421 cgcagagttt aaacgcgaat ccgctcaact ggtcgttgac cagaaataca ccgtggcaga
      481 tgcagccagc gctatggatg tcggcctttc cacaatgacg cgatgggtga aacaattacg
      541 tgatgagcgg cagggaaaaa caccaaaagc ctcccccatt accccggaac aaattgaaat
      601 ccgtgagctc aggaaaaagc tacaacgtat tgaaatggaa aatgaaatat taaaaaggct
      661 accgcgctct tgatgtcaga ctccctgaac agttctcgat aatcgggaaa ctcagagcgc
      721 attatcctgt ggtcacactc tgcca
//
LOCUS       DAJVPT010000336          729 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVPT010000336 DAJVPT010000000
VERSION     DAJVPT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..567
                     /locus_tag="OQM47_004233"
     CDS             <1..567
                     /locus_tag="OQM47_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1482122.1"
                     /translation="VLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVGLGLKGQWRD
                     AARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPLGALDALTRL
                     EMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVDIPRPRRLGS
                     VRLAELEAEVLQRVMRRGHSEQLIRRHG"
BASE COUNT          160 a          162 c          240 g          167 t
ORIGIN      
        1 gtgttagcgg gcaccacacc gctggctgaa attcaggatg atacgcgaat gatgtttcag
       61 gatgcgcgtc tgctgccgtg gaaatcggtg attgataacg ttgggttagg ccttaaaggt
      121 cagtggcgcg atgccgcgcg tcgggcgttg gctgcggcag gactggagaa tcgcgccggg
      181 gaatggcctg ccgcactttc tggcgggcag aaacagcgtg tggcgctggc aagagcgttg
      241 attcatcgac cgggtttatt gttgcttgat gaaccgctcg gggcactgga cgccttaacg
      301 cgactcgaga tgcaggattt gattgtgtca ctttggcagc agcacggctt taccgtactg
      361 ttggtgacgc atgatgtgag cgaagcggta gcaatggctg accgggtgtt gttaattgaa
      421 gagggaaaaa ttagtctgga tttgacagtg gatatccccc gtccgcgtcg attagggtcg
      481 gtgcgattag cggagctgga agcggaagta ttgcaacggg taatgcggcg tgggcattct
      541 gaacaactga ttcggcgtca tggttaatat gccggatgtg gcgcacaaaa ccagcatttt
      601 caacggactt gtaggcctga taagacacgt caagcatcgc atcaggcagt tgggcacggc
      661 tgccggatgc ggcgtgaacg ccttatccag cctacaaaac cagcaatttc aacggagttg
      721 taggcctga
//
LOCUS       DAJVPT010000337          714 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVPT010000337 DAJVPT010000000
VERSION     DAJVPT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..584
                     /locus_tag="OQM47_004234"
     CDS             <1..584
                     /locus_tag="OQM47_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HCS1482123.1"
                     /translation="LAGVAVPSVITAENGTVFRENLLFTHRGLSGPAVLQISSYWQPG
                     EFVSINLLPDVDLETFLNEQRNAHPNQSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQ
                     LNVCDQQALISTLTDWRVQPNGTEGYRTAEVTLGGVDTNELSSRTMEARKVPGLYFIG
                     EVMDVTGWLGGYNFQWAWSSAWACAQDLASIVK"
BASE COUNT          157 a          179 c          197 g          181 t
ORIGIN      
        1 tgctggcggg cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg
       61 agaacttact cttcacccac cgcggcttat ctggaccggc ggtgttgcag atttcaagct
      121 actggcaacc gggtgaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct
      181 tcctgaatga gcagcgtaac gcacatccga atcaaagcct gaaaaacaca ctggcggttc
      241 atctaccgaa gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc
      301 taaaacagct caacgtgtgt gaccaacagg cactgattag cacattgacc gactggcgcg
      361 tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca
      421 ccaacgaact ttcttcacgg acgatggaag cgcgcaaagt gcctgggctg tacttcatcg
      481 gcgaagtgat ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga
      541 gtgcatgggc ttgtgcgcag gatttggcgt caattgtgaa ataaccatga cgttttcccg
      601 tcagccgttt gtatatgtcg ttattaagtc attttggtac ggttctgcat ccgacattct
      661 ggtgttaact cgctgttttg ttagcgaatt aacgctattg ccgttgtggt tatt
//
LOCUS       DAJVPT010000338          713 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVPT010000338 DAJVPT010000000
VERSION     DAJVPT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..211)
                     /locus_tag="OQM47_004235"
     CDS             complement(<1..211)
                     /locus_tag="OQM47_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="membrane spanning protein in TonB-ExbB-ExbD
                     complex; transduces proton motive force of the cytoplasmic
                     membrane to outer membrane transporters; involved in the
                     transport of ron-siderophore complexes, vitamin B12 and
                     colicins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1482124.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEP"
     gene            426..>713
                     /locus_tag="OQM47_004236"
     CDS             426..>713
                     /locus_tag="OQM47_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1482125.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKK"
BASE COUNT          198 a          163 c          177 g          175 t
ORIGIN      
        1 ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg
       61 tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga
      121 gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc
      181 ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa ctaggcctcg
      241 attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat
      301 gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac atcatctttc
      361 cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg
      421 tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt
      481 ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga
      541 cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct
      601 caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc
      661 cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaa
//
LOCUS       DAJVPT010000339          703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVPT010000339 DAJVPT010000000
VERSION     DAJVPT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..703
                     /locus_tag="OQM47_004237"
                     /pseudo
     CDS             1..703
                     /locus_tag="OQM47_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          177 a          185 c          165 g          176 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga aca
//
LOCUS       DAJVPT010000340          703 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVPT010000340 DAJVPT010000000
VERSION     DAJVPT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          194 a          170 c          150 g          189 t
ORIGIN      
        1 gcaatcccgg cacccgtttt atgaggtgca ggtgcactat aattccaccc gttctggttt
       61 tcaatagcta cattcaacat tttctctttc ctttcatcac cgaagtgaac tttgttgatg
      121 cggtgcctgg tgcctccagg tgacgttaac cagttaacaa ttaacgccgg atacagggaa
      181 acccataacc acccgatacg tgggaactaa tcgcttttta actgttccgc gtgcgctcag
      241 ccgcattcac cgcatcacaa aattcacttt aaaaagggcg gacatcagcc agcaatgaaa
      301 ctgatgccgc caaaggtacc aatcaacatg gagtgttgtg gcgtggttgt cacttaagcg
      361 tatggtcaac ctgacaaccc ggtgtcctca acggggaagg aataactccg ccatacttac
      421 cgccgcgtca tttcgcgttg tgtgcctgct tttaactacg tcaggcgagg tggttcctgt
      481 tattccccaa caacaagaaa tttgtataat ctgaataccc caacaataag aagagtgagc
      541 aagtgatgta ttagcgatgt catctctcaa agagactgca tcaatgttaa aattattcaa
      601 tgaagcaaaa actcaggctg aggttgatcg cgctgtgtct gaaataaact ctaagttgtc
      661 aagcattcag cttgaatatt tgtcatctca ttgatgagct gac
//
LOCUS       DAJVPT010000341          697 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVPT010000341 DAJVPT010000000
VERSION     DAJVPT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>697
                     /locus_tag="OQM47_004238"
     CDS             <1..>697
                     /locus_tag="OQM47_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCS1482126.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGG"
BASE COUNT          175 a          203 c          199 g          120 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      601 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct gccatctgct
      661 gacgtcgatg acggacgcca tgcgcttccc cggagga
//
LOCUS       DAJVPT010000342          681 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVPT010000342 DAJVPT010000000
VERSION     DAJVPT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..157)
                     /gene="ssuB"
                     /locus_tag="OQM47_004239"
     CDS             complement(<1..157)
                     /gene="ssuB"
                     /locus_tag="OQM47_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="part of the ABC type transport system SsuABC for
                     aliphatic sulfonates; with SsuA being the periplasmic
                     substrate-binding subunit, SsuB the ATP-binding subunit
                     and SsuC the permease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1482127.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKST"
     gene            complement(154..>681)
                     /gene="ssuC"
                     /locus_tag="OQM47_004240"
     CDS             complement(154..>681)
                     /gene="ssuC"
                     /locus_tag="OQM47_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1482128.1"
                     /translation="LSRWGERLLDTSIQMLRNVPHLALIPLVILWFGIDESAKIFLVA
                     LGTLFPIYINTWHGIRNIDRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALG
                     LMWLTLIVAETISANSGIGYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERL
                     WLRWNPAYHLKEATI"
BASE COUNT          185 a          195 c          167 g          134 t
ORIGIN      
        1 gggtactttt accaccaccg ctgcggccca ccaccgccac aaactgacct gccggaatat
       61 gtaaatccag ttggttcagg acgatatttt ccgcgtaatg tttgcttact gcattgagca
      121 acaatggtgt gccctggttc agacgagcag tattcatatc gtggcctcct tcaaatgata
      181 agccgggttc cagcgcaacc agaggcgctc taacagctgc gcgctgacgt cagcaagttt
      241 gccgagcagg gcgtaaagaa taatagcgac taccaccacg tctgtttgca aaaactctcg
      301 cgcattcatc gccagataac caatgccgga attggcagaa atggtttcgg caacaatcag
      361 cgtcagccac atcaggccca acgcaaaacg cacgccgacc ataattgagg gcagggcatc
      421 gggcaggatc acatggataa acagcggtat gccggataat ccatagctac gcgccatctc
      481 caccagcccg cgatcgatat tacggatgcc atgccaggtg ttgatataaa tggggaacag
      541 cgtaccgagc gccaccagaa agattttcgc ggattcatca atgccaaacc acaaaatcac
      601 cagcgggatc agcgccagat gcggcacgtt gcgcaacatc tgaattgagg tatccagcag
      661 cctttctccc cagcgcgaca g
//
LOCUS       DAJVPT010000343          668 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVPT010000343 DAJVPT010000000
VERSION     DAJVPT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>668
                     /locus_tag="OQM47_004241"
     CDS             <1..>668
                     /locus_tag="OQM47_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCS1482129.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJVPT010000344          649 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVPT010000344 DAJVPT010000000
VERSION     DAJVPT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(24..>649)
                     /locus_tag="OQM47_004242"
     rRNA            complement(24..>649)
                     /locus_tag="OQM47_004242"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJVPT010000345          641 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVPT010000345 DAJVPT010000000
VERSION     DAJVPT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..539
                     /locus_tag="OQM47_004243"
     CDS             <1..539
                     /locus_tag="OQM47_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1482130.1"
                     /translation="LSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEF
                     NRLNFKPVTYTDAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAEL
                     RQNNTPPADKMIPGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTL
                     VNRGAIKNMTVEQLLSLK"
BASE COUNT          182 a          163 c          138 g          158 t
ORIGIN      
        1 ccctttctca ccctgacgca accatcgaaa atggtcgcgc tgtcactacg tctgttgcgg
       61 ttgcggagtt cttccggaaa atgcacaaaa acgttattca gaagatcgaa actctcgaat
      121 gctcgccgga gtttaaccgg cttaatttta agcccgtcac ctataccgac gccaaaggcg
      181 aaaaacgccc aatgtaccaa atcaccaaaa acggcttcgt tttcctggtg atgggcttca
      241 ctggcaaaaa agccgccgca ttcaaggaag cctacattgc tgagttcgac cgcatggagg
      301 ccgaactgcg ccagaataac accccacctg cagacaagat gattccgggt gatggtcgca
      361 ctctggttgt tcacttcgac aaattcggca atgtcgaatt caccgaaacc gttcctgatg
      421 gcgcactggt ctgtaccctg gagactttcc ggttttatct ggaaaaacag ggatggactc
      481 ttgttaaccg gggcgcaatt aaaaatatga ctgtggaaca attactttct ttaaaataaa
      541 aatggagcta cggatatgaa ccctatgctt gattattgtt ttacctctgc acctcagcag
      601 accataacac acaccggaat caaaaatttg ttatggataa t
//
LOCUS       DAJVPT010000346          634 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVPT010000346 DAJVPT010000000
VERSION     DAJVPT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..286
                     /locus_tag="OQM47_004244"
     CDS             <1..286
                     /locus_tag="OQM47_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482131.1"
                     /translation="NLQNTDAAAGMTKELAQEGEITERDKVIFAAYQTSGSAIITNYF
                     SSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEERRNPTQGAQA"
     gene            283..>634
                     /locus_tag="OQM47_004245"
     CDS             283..>634
                     /locus_tag="OQM47_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1482132.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNV"
BASE COUNT          144 a          174 c          169 g          147 t
ORIGIN      
        1 taacctgcaa aacacggatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga
       61 aattaccgaa cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat
      121 caccaactac ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt
      181 acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct
      241 taacttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc
      301 aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc
      361 aacctgctgc caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc
      421 ctgctcgact gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt
      481 gaagcggcaa ccgtcctttt agccgcatta atgagtatgg gcggcgcagt cggtgttgcc
      541 gccagtctgg caaccgctga cgcattaacc ggccacgatg tcaccgtttt actgcccgca
      601 atgtatttaa tgggcaaccc ggtgcaaaat gtcg
//
LOCUS       DAJVPT010000347          632 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVPT010000347 DAJVPT010000000
VERSION     DAJVPT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>632
                     /locus_tag="OQM47_004246"
     CDS             <1..>632
                     /locus_tag="OQM47_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1482133.1"
                     /translation="EVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVT
                     TVFQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEM
                     TPIGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAA
                     GQADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSG"
BASE COUNT          187 a          175 c          158 g          112 t
ORIGIN      
        1 tgaggtttac atcttcccga ccttcaaaca aatgaagacc tacgatgagc cattcagctt
       61 ccagatgagt gacggtacaa ccatcggcta tcacatcggc gtggcctaca aagttgatcc
      121 atccaaagtt accacagtct ttcagaccta ccgcaaaggc gtggacgaca ttaccgacac
      181 cgacctgcgc cagaagatag ccgacgcact caaccgactg gccagcaaaa tgaccaccga
      241 caaatttatc gacggcggca agtctgagct gctggatgca gctcttaaag acattcagga
      301 agaaatgacg cccatcggta ttcaggtaat gagcctctca tatgtgggta agccggagta
      361 cccgcctact gttatcgaca gcattaatgc caaagtcacg gcgaaccaga aaaccctgca
      421 atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac atgttgcgcg cggaagctgc
      481 cggacaggca gatgcgattc gcacaaaagc ccaggccgaa gccgatgcta ttcgtttacg
      541 cggtgaagct ctgcgccaga acccaaacgt tatggagctg gaagccatca acaagtggaa
      601 cggtacactg ccgcaataca tgaccagcgg tg
//
LOCUS       DAJVPT010000348          626 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVPT010000348 DAJVPT010000000
VERSION     DAJVPT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            19..492
                     /locus_tag="OQM47_004247"
     CDS             19..492
                     /locus_tag="OQM47_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1482134.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          206 a          111 c          126 g          183 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcag
//
LOCUS       DAJVPT010000349          625 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVPT010000349 DAJVPT010000000
VERSION     DAJVPT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..595
                     /locus_tag="OQM47_004248"
     CDS             <1..595
                     /locus_tag="OQM47_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCS1482135.1"
                     /translation="LLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIE
                     ALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDAD
                     MPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVI
                     APIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT          152 a          155 c          175 g          143 t
ORIGIN      
        1 gctgttgatg ctggtggcga agctgtctcg cctgaaaggt ctggatgcct ccattgaaca
       61 tgacattgtg catggtctgc aggcgctgcc gagccgtatt gagcagatgc tgtctcagga
      121 taaacgcatt gaagctctgg cagaagattt ctctgacaaa catcacgcgc tgttcctggg
      181 ccgtggcgat cagtacccaa tcgcgctgga aggcgcattg aagctgaaag agatctctta
      241 cattcacgct gaagcctacg ctgcaggcga gctgaaacac ggcccgctgg cgctgattga
      301 cgcggatatg ccggttatcg tcgttgcacc gaacaacgaa ctgttggaaa aactgaaatc
      361 caacattgaa gaagttcgcg cgcgtggcgg tcaattgtat gtcttcgccg atcaggatgc
      421 gggttttgta agtagcgata acatgcacat catcgagatg ccgcatgtgg aagaggtgat
      481 tgcaccgatc ttctacaccg ttccgctgca gctgctggct taccatgtcg cgctgatcaa
      541 aggcaccgac gttgaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaatcact
      601 atcatagata aaaaagggct gccga
//
LOCUS       DAJVPT010000350          610 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVPT010000350 DAJVPT010000000
VERSION     DAJVPT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(4..534)
                     /gene="cybB"
                     /locus_tag="OQM47_004249"
     CDS             complement(4..534)
                     /gene="cybB"
                     /locus_tag="OQM47_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1482136.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
BASE COUNT          182 a          156 c          126 g          146 t
ORIGIN      
        1 ccttcaggaa cgtttacgcg gcatcatgcg tagaagtgtg ttgtccttcc agaaatagtg
       61 gtgtgccagt gcagccgcag cgtgcaaccc gatgacaaaa tatcccagat tcgccagcgt
      121 ctcatgccac gactttaagc tatcaacccg ttcgaaattg gcctctgaag cgtaaggcat
      181 cgtcaaacca aacgcaaacc acgggttgcc ccggttatac atcatcacca aaccaatcac
      241 tggcagcgca ataaacagga gataaatcac caaatgtccc aaatgcgcca gtcccgtcat
      301 catcggtttc ggcttaggta taatcggcgg ggttgggtat ttcagtctta acaaaagacg
      361 aaccaccatc agcacgagaa ttgagatgcc acaggaaaca tgaatcatgt tgatgagcgg
      421 ccgatcgcta cgtgggaaga aaccacgaaa ctccattgcg caatacgctg cgataaccag
      481 caaaaagacc agccagtgaa tgctgatttg taaccttgag tatttatttt ccataacatt
      541 tcctgcttta acataatttt ccgttaacat aacgggcttt tctcaaaatt tcattaaata
      601 ttgttcaccc
//
LOCUS       DAJVPT010000351          605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVPT010000351 DAJVPT010000000
VERSION     DAJVPT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..159)
                     /locus_tag="OQM47_004250"
     CDS             complement(<1..159)
                     /locus_tag="OQM47_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482137.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLN"
     gene            complement(497..>605)
                     /locus_tag="OQM47_004251"
     CDS             complement(497..>605)
                     /locus_tag="OQM47_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCS1482138.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          155 a          141 c          128 g          181 t
ORIGIN      
        1 attgagccct tttcttgcgg cggtaaccca ttgtcctaaa gtgccttcag gaacagataa
       61 tcgggaagcg ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc
      121 tttaaactcc gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca
      181 ttctatttca ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga
      241 gtcaagttcc cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat
      301 tccccggagg caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga
      361 aagagggacc atctccatct cattaaatgg accacgccag acaattcact gtcatgctga
      421 tgctgcactg tggggacaga atcactcatg atgacttcga taatcgacga cattattatt
      481 tggccgagct tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata
      541 aaaaatcaat catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac
      601 tattt
//
LOCUS       DAJVPT010000352          605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVPT010000352 DAJVPT010000000
VERSION     DAJVPT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            119..304
                     /locus_tag="OQM47_004252"
     CDS             119..304
                     /locus_tag="OQM47_004252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482139.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            352..605
                     /locus_tag="OQM47_004253"
                     /pseudo
     CDS             352..605
                     /locus_tag="OQM47_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a          127 c          188 g          146 t
ORIGIN      
        1 ccggaggaaa cagaccagac ggtttaaatg agccggttac gaagaagctg aggttctgcg
       61 ctttgataat tttgacgatg ccgttgagtg ttgcgcagac ctgaatattc cgttctttgt
      121 ggatgccgga aacaaaaaac tggtcttctg gtttgtacgt gttgatgacg aagggtatcc
      181 ggaaattgcc cgctgcatgg agcgggaatt tgcgacaatt cctgccggta tcaacgccga
      241 cggcatgtat tgccctgaat gcggtacggt tcactggccg gatggcgtta ttccgccttt
      301 ctgatgcttc cccgttttgc cgatattttt cagcagggaa accgatggct taactggctg
      361 gagaagcagc cggtgcagat gagccgcctg gaacattatg ccggtcagga tgaaattggc
      421 ctgcgttaca acagccaccg gacaaaacga gaagaaaacc ttgtgatgag tggcgacgag
      481 tttatggaaa ggttctcctg gcatgtggcg gataaggggt tccggatggt gatacggggt
      541 cctgagtccg gtgaagcggc gattactgga agatgtggtg tacgccataa cggagacagt
      601 gagaa
//
LOCUS       DAJVPT010000353          590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVPT010000353 DAJVPT010000000
VERSION     DAJVPT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..354)
                     /locus_tag="OQM47_004254"
     CDS             complement(<1..354)
                     /locus_tag="OQM47_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCS1482140.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFT"
     gene            complement(412..>590)
                     /locus_tag="OQM47_004255"
     CDS             complement(412..>590)
                     /locus_tag="OQM47_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1482141.1"
                     /translation="YLGVHWPLDMLGGLLAGMIGCLSAQIIWQAMGHKLYQRLQSWYR
                     VCFALPIRKGWVRD"
BASE COUNT          148 a          158 c          145 g          139 t
ORIGIN      
        1 ggtaaaaggg atttcattat caatcgcttc aataatccac ggcgtggtgg tgccacagtc
       61 aaaaaagagg gtctgatcgg gttctaccag tgtcgcagcc agttttgccg cccggcgttt
      121 ttcttccacc aggcgggatt tttgatcgct taacaggtaa tggctggcac tgcgcggttc
      181 cagaacaata tagccgccga gcaaaacgac gggcgcactg tggttgttca gatcgcgacg
      241 aatcgtcatc tccgaaaccc caagcagggc ggcggcgtct ttaagatgta acttatcgct
      301 gcgttttaat tcttgcagca gctgcccgat acgctcttcg cgacgtgttt ccataatccc
      361 tctgaatagt tattgaagcg agccgctcaa tactacactt tttagcagag atcagtcacg
      421 cacccagcct ttgcggatcg gtaatgcaaa acagacgcga taccacgatt gcagacgttg
      481 atagagttta tgccccatcg cttgccagat aatctgggca ctaaggcagc caatcatacc
      541 tgccagcaat ccaccgagca tatccagcgg ccagtggacg ccaagataaa
//
LOCUS       DAJVPT010000354          588 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVPT010000354 DAJVPT010000000
VERSION     DAJVPT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..259
                     /locus_tag="OQM47_004256"
     CDS             <1..259
                     /locus_tag="OQM47_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1482142.1"
                     /translation="HYEALFVYAQKRLDKCVFGEEKPACKQCPVHCYQPAKREEMKQI
                     MRWAGPRMLWRHPILTVRHLIDDKRPVPELPEKYRPKKPRE"
     gene            complement(296..>588)
                     /locus_tag="OQM47_004257"
     CDS             complement(296..>588)
                     /locus_tag="OQM47_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="regulates the expression of formate dehydrogenase H
                     and hydrogenase-3 structural genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1482143.1"
                     /translation="NVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQ
                     EGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI"
BASE COUNT          136 a          157 c          158 g          137 t
ORIGIN      
        1 gcattacgag gcgttgtttg tttacgcgca aaagcggctg gataaatgtg tgttcggcga
       61 agagaaacca gcctgtaagc agtgtccggt gcactgttac cagcctgcaa agcgtgaaga
      121 gatgaaacag attatgcgtt gggccgggcc gagaatgctg tggcgtcatc cgatcttaac
      181 cgtgcgtcat ctcatcgatg acaagcgtcc ggtgccagaa ctgccggaaa aatatcgtcc
      241 gaaaaaacct cgtgaatagt atgccggaca ggcagatctg tccggcaatt tgcagttaaa
      301 tcaatgccga tttatcaatt cccagccgct tcatccgtga cagcagggtc gtgcgtttca
      361 gccccagacg ttgcgcagcg cctttaggcc cggcaaccac gctgttggtt tctttcagca
      421 cgcgcacaat caactgatat tcatcttcgc cctcctgggc gacaaccgtt gcggcaggcg
      481 gcgtttcagg ctccggtaaa gcaatatctg gcaatgacag ctgcagcacg ttgccgcgtg
      541 ttagcaatac tgcgcgctca atgacgtttt ccagttcgcg tacgttac
//
LOCUS       DAJVPT010000355          588 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVPT010000355 DAJVPT010000000
VERSION     DAJVPT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..337)
                     /gene="sdaC"
                     /locus_tag="OQM47_004258"
     CDS             complement(<1..337)
                     /gene="sdaC"
                     /locus_tag="OQM47_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1482144.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPI"
BASE COUNT          165 a          136 c          145 g          142 t
ORIGIN      
        1 ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg ccaataccaa
       61 agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac agtacgaagc
      121 gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc atgatgatca
      181 gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc gcgccgattg
      241 ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca ctacgagagt
      301 ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg aggaatgcaa
      361 ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa atgaaatctg
      421 tctatcggcg atggcttaat catttaccgc cgtaataatt tgagggcgga aagatacaca
      481 caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt ggaagataga
      541 ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaag
//
LOCUS       DAJVPT010000356          585 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVPT010000356 DAJVPT010000000
VERSION     DAJVPT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          210 a          104 c          122 g          149 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 gggggggggg atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga
      541 tcttgttgat ggaaaaacga atccgaataa agcaacaaaa gcatt
//
LOCUS       DAJVPT010000357          581 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVPT010000357 DAJVPT010000000
VERSION     DAJVPT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..177
                     /locus_tag="OQM47_004259"
     CDS             <1..177
                     /locus_tag="OQM47_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1482145.1"
                     /translation="HEFYEAELKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQ
                     WLVEYTQQKLSLAS"
     gene            complement(383..>581)
                     /locus_tag="OQM47_004260"
     CDS             complement(383..>581)
                     /locus_tag="OQM47_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482146.1"
                     /translation="IAMLGAGLAAIPEVCNLLFTPTIYLLLNIFMLTLGALMLFFSGR
                     VGNVPEFCYVGYDGVGFAVIS"
BASE COUNT          177 a          141 c          137 g          126 t
ORIGIN      
        1 catgagttct acgaagcgga actgaaatac ctggtggatc acgaatgggt ccgccgcacc
       61 gacgatgccc tgtggcgtcg cacaaaacaa ggcatgtggc tgaatgcgga tcaacaatct
      121 cgtgtgagtc agtggctggt ggaatatacg cagcagaagt tgtcgctggc gtcgtaaatt
      181 aacgtaaggt gatcaggtca gatttcaatc tggcctgaga ctgatgacaa acacaaaact
      241 gcctgatgcg ctacgcttat caggcctacg tggtttatgc aatatattga atttgcatgg
      301 tcttgtaggc cagataagac gttcacgtcg catccggcat gaacaaagcg cactttgtca
      361 aaaatctaac ctacttttta attcaggaaa ttaccgcaaa gcccacgcca tcatacccaa
      421 cgtaacaaaa ctcaggcacg ttccccactc gccccgagaa aaatagcatt aatgcgccca
      481 gcgtcagcat aaaaatattg agcagaaggt aaattgttgg cgtaaagagt aaattgcaaa
      541 cctcaggaat agcggcaagc ccagccccca gcatggcaat a
//
LOCUS       DAJVPT010000358          581 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVPT010000358 DAJVPT010000000
VERSION     DAJVPT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..410
                     /locus_tag="OQM47_004261"
     CDS             <1..410
                     /locus_tag="OQM47_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1482147.1"
                     /translation="QPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAVTI
                     SEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAFAW
                     LAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            508..>581
                     /locus_tag="OQM47_004262"
     CDS             508..>581
                     /locus_tag="OQM47_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCS1482148.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
BASE COUNT          125 a          145 c          157 g          154 t
ORIGIN      
        1 cgcaacctgc accgaaaagc accggacgcg aatactttaa ctacggctgg ctggagcgcc
       61 atttacgcca ttttccgggt gttgatcccc gtgatgttca ggccacactg gcagaactca
      121 ccgccgtgac catttctgaa caagttttgt tgagcggtgg ctgcgaacga ttgatggtat
      181 gtggtggagg tagtcgtaat ccgctactca tggcgcgtct ggcggtatta ctgctaggca
      241 cagaagtcac caccaccgat gccgttggca ttagtggcga tgacatggaa gcattggctt
      301 tcgcctggct tgcctggcgg acgctggcgg gattaccagg aaatctgcct tccgtcactg
      361 gcgcaagcca ggagacggta ctgggggcta ttttccccgc caactcgtga tatcagaatt
      421 aactgaattt tttcttatcg tgtctgccgt tacacttgga agattaagga gggtatttgc
      481 cctccggacc aggaaagtct tcgggatatg actatgaaaa aactgttaat tatcattttg
      541 ccggtccttc tctccggttg tagcgccttt aatcaactgg t
//
LOCUS       DAJVPT010000359          578 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVPT010000359 DAJVPT010000000
VERSION     DAJVPT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..296)
                     /locus_tag="OQM47_004263"
     CDS             complement(<1..296)
                     /locus_tag="OQM47_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1482149.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPA"
BASE COUNT          156 a           78 c          134 g          210 t
ORIGIN      
        1 gcaggcaatg gggagattaa gcttaaatta tttttactta tattaattga tttaattccc
       61 ggggggattt caggtaatgt tgtcaggcct aattcagaca agtccaggtt cgtctcttgg
      121 ttttgtagac atgatactag tcgctgaaaa gcgatgtctc gttgttcttc ttgtatgcgg
      181 ttatttttcc attcagtcca atgggttaga taattttcat atgcgcggct agtgtcgatt
      241 gaataagtgg agaacgaatt tgaaattaat ctgtgattgt tatttgtcgg gagcataaat
      301 atcagggtgt attctttatg ttgagaggtg ttggattctt ttttatctgc tttgcaatgt
      361 tattggttcc cttcagcagt agacaggaac ttctggagga ggcggatata tagtttgtgt
      421 aagcatatta actccatatg ttatatattg aagaacttct gctttacact atcctaaact
      481 ggatcggttt aatgtaaaaa aacgatactt tttggggggg ggcaattttt ccttaaggta
      541 tgaaaaaagg agctgaagct atataatagc tattaatg
//
LOCUS       DAJVPT010000360          576 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVPT010000360 DAJVPT010000000
VERSION     DAJVPT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(212..>576)
                     /locus_tag="OQM47_004264"
     CDS             complement(212..>576)
                     /locus_tag="OQM47_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1482150.1"
                     /translation="LEAAREARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLS
                     PVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMK
                     EELSGLDDLASIRAFDVE"
BASE COUNT          118 a          193 c          129 g          136 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacggaggc ccgcccccgg gcctcccctg attcatccgt ttccctgttc
      121 agcctgacag cacgccccgg ctgccggatg acagactccg cttcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attactcaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagaccgctc agctcctctt tcatttcacg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgtttgt gctgtgacag
      421 attttgccag catgactacc ggggacaggc ggcccagtga gtcggggcca gcattccaga
      481 tacgaccgtt ccattcaaac gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa
      541 tttcctgacg tctggcctct ctggccgctt ccagca
//
LOCUS       DAJVPT010000361          570 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVPT010000361 DAJVPT010000000
VERSION     DAJVPT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..444
                     /locus_tag="OQM47_004265"
                     /pseudo
     CDS             1..444
                     /locus_tag="OQM47_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          125 a          167 c          158 g          120 t
ORIGIN      
        1 gaagccctga agcttgctga acgcatcggt gttgctgccg cagcccgtga actcagcctg
       61 tatgaatctc agctctatgc gtggcgcagc aaactgcagc aacagatgac ctcgtctgag
      121 cgtgaaagtg aactgcccgc ctgaagcggc aactggcgga gcaggcagag gaactggcta
      181 tcctccaaaa ggccgcgaca tactttgcgc agcggctggt atacgtggtg tcagcgtcgg
      241 acaggaataa gccctcgtca gcagttccgt cagcactgcg acagcgttgt cctcgcggcg
      301 gcctttaccc ggtcaaaaca gcgttacggt gcaccacgcc tgacggatga actgcgtgct
      361 cagggttacc actttaacgt aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg
      421 gcaaaggcct cccggaagtt cacgtatagg aaattgaaaa accagacgat accgctgtcc
      481 acgccttatg ccacgtgagc ttttctgctt ttccctccac tccggtgttt tctctctctg
      541 catggtcgat tcttataccg caacaggctg
//
LOCUS       DAJVPT010000362          566 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVPT010000362 DAJVPT010000000
VERSION     DAJVPT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>566
                     /gene="tuf"
                     /locus_tag="OQM47_004266"
     CDS             <1..>566
                     /gene="tuf"
                     /locus_tag="OQM47_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1482151.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLL"
BASE COUNT          128 a          140 c          164 g          134 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgct
//
LOCUS       DAJVPT010000363          564 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVPT010000363 DAJVPT010000000
VERSION     DAJVPT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>564
                     /locus_tag="OQM47_004267"
     CDS             <1..>564
                     /locus_tag="OQM47_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCS1482152.1"
                     /translation="DVKQAVTHACVINQAAATGGDARQLVGYLVSQSGLPLDTSALQA
                     QLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAPKAGSETIIAA
                     AFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVMVASTVAKLAT
                     IIDGEEDSSRRMGFETILPLREGNGPT"
BASE COUNT          113 a          152 c          180 g          119 t
ORIGIN      
        1 cggatgtcaa acaagccgtt acccacgcct gtgtgattaa ccaggcggca gccactggtg
       61 gtgatgcgcg tcagttggtg ggctatctgg tatcgcaatc gggcctgccg ttggatacca
      121 gcgcattgca ggcgcagctt cgcgaaacat tgccgccgca tatggtgcca gtcgttctgc
      181 tgcaactgcc gcagttgcca cttagcgcca acggcaagct ggatcgcaaa gccttaccgt
      241 tgcctgaact gaaggcacaa gcgccagggc gtgcgccgaa agcgggcagt gaaacgatta
      301 tcgccgcggc attcgcgtcg ttgctgggtt gtgacgtgca ggatgccgat gctgatttct
      361 ttgcgctggg cggtcattcg ctactggcaa tgaaactggc agcgcagtta agtcgacagt
      421 ttgctcgtca ggtgacgccg gggcaggtga tggtcgcgtc aaccgtcgcc aaactggcaa
      481 cgattattga tggtgaagag gacagctccc ggcgcatggg attcgaaacc attctgccgt
      541 tgcgtgaagg taatggcccg acgc
//
LOCUS       DAJVPT010000364          561 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVPT010000364 DAJVPT010000000
VERSION     DAJVPT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>561
                     /locus_tag="OQM47_004268"
     CDS             <1..>561
                     /locus_tag="OQM47_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1482153.1"
                     /translation="YHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEE
                     LTDNAGIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGT
                     GKNHLAVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLL
                     IIDEVGVQFGSAAEMAILQEIINARY"
BASE COUNT          144 a          121 c          161 g          135 t
ORIGIN      
        1 aatatcacac gcatatactg gtgtggagcg atcgtaatgg cgaggacaga gaagaaattt
       61 catgctgccc tgactgctta atcgctgagg ccaacgattt gaccatggag ctgtcgtcca
      121 tcaaggcgga agagctgact gataacgccg gaattgccct gcgttttcgg gactgcgagt
      181 ttgataatta tctggaggtt aatcctggcg cagccagaaa tcttgcggcc tgtcgccgct
      241 atgcggagaa ctggccagat atgctggaga acggtaccag tcttgttatg accggcagtt
      301 gtggtaccgg aaaaaatcat ctggctgtgg ctatggcaaa acacatcatc cgtaactatc
      361 tggccagtgt ggagatcacc gacgtgatgc gccttacccg tgctgtgaaa aattgctggc
      421 ggaatgacag cgaaaaaaca gcggatgaag ttattgagcg ttatgcgtca atggatttgc
      481 tgatcatcga cgaagttggc gttcagtttg gcagcgcggc tgaaatggct attttgcagg
      541 aaattatcaa cgccaggtac g
//
LOCUS       DAJVPT010000365          555 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVPT010000365 DAJVPT010000000
VERSION     DAJVPT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..165
                     /gene="relB"
                     /locus_tag="OQM47_004269"
     CDS             <1..165
                     /gene="relB"
                     /locus_tag="OQM47_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCS1482154.1"
                     /translation="TPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNP
                     KPVRVTLDEL"
     gene            165..452
                     /gene="relE"
                     /locus_tag="OQM47_004270"
     CDS             165..452
                     /gene="relE"
                     /locus_tag="OQM47_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCS1482155.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          150 a          116 c          149 g          140 t
ORIGIN      
        1 actccttctg aagcgcttcg tctcatgctc gagtatatcg ctgacaatga acgcttgccg
       61 ttcaaacaga cactcctgag tgatgaagat gctgaacttg tggagatagt gaaagaacgg
      121 cttcgtaatc ctaagccagt acgtgtgacg ctggatgaac tctgatggcg tattttctgg
      181 attttgacga gcgggcacta aaggaatggc gaaagctggg ctcgacggta cgtgaacagt
      241 tgaaaaagaa gctggttgaa gtacttgagt caccccggat tgaagcaaac aagctccgtg
      301 gtatgcctga ttgttacaag attaagctcc ggtcttcagg ctatcgcctt gtataccagg
      361 ttatagacga gaaagttgtc gttttcgtga tttctgttgg gaaaagagaa cgctcggaag
      421 tatatagcga ggcggtcaaa cgcattctct gaaccaaagc atgacatctc tgtttcgcac
      481 cgaaggtgac acttctgctt tgcgttgaca ggagaagcag gctatgaagc agcaaaaggc
      541 gatgttaatc gccct
//
LOCUS       DAJVPT010000366          555 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVPT010000366 DAJVPT010000000
VERSION     DAJVPT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>555
                     /locus_tag="OQM47_004271"
     CDS             <1..>555
                     /locus_tag="OQM47_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCS1482156.1"
                     /translation="LNISSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISCGVDQ
                     LIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKGRVDMT
                     MALGLSDIKAQMGGLVYRGFVTGNGFKRLGDTLRYLQAIEKRLEKLAVDPHRDRAQML
                     KVENVQQAWQQWINKLPPARREDED"
BASE COUNT          147 a          124 c          164 g          120 t
ORIGIN      
        1 ctaaatattt cctcgccgat caagtattta catgagaagt taccaaacaa agccaagctg
       61 gggctgtact ttaacccgta tggcaaagtg ctggagctga tcgacgactg tatctcctgc
      121 ggtgtggatc aactgatcga cgccaatggt ggcccggtct ggacggaaga aggctttgct
      181 gcgctgcatg aaaaagtgcg cgccgaactg aatgacacgg tggtggatat tgcgaagcag
      241 gtcgagcaaa tcctcactgc ggtgttcaat atcaacaaac gtctgaaagg gcgggtggat
      301 atgaccatgg cactggggct ttctgatatt aaagcgcaga tgggcgggtt ggtatatcgc
      361 ggttttgtca ctggtaacgg cttcaaacgg ctgggcgaca cgctgcgata tttgcaggcg
      421 attgaaaaac gactggaaaa actggcggtt gatccacatc gcgatcgtgc acagatgctg
      481 aaagtcgaaa acgtccagca ggcgtggcag caatggatca acaaactgcc gcctgcacgt
      541 cgtgaggatg aagac
//
LOCUS       DAJVPT010000367          548 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVPT010000367 DAJVPT010000000
VERSION     DAJVPT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..513
                     /gene="glyS"
                     /locus_tag="OQM47_004272"
     CDS             <1..513
                     /gene="glyS"
                     /locus_tag="OQM47_004272"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="glycine--tRNA ligase beta chain; glyS; class II
                     aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2);
                     catalyzes a two-step reaction; first charging a glycine
                     molecule by linking the carboxyl group to the
                     alpha-phosphate of ATP; second by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1482157.1"
                     /translation="VVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDAR
                     MKAVSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVL
                     RDKLEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELF
                     LRVADISLLQ"
BASE COUNT          122 a          133 c          161 g          132 t
ORIGIN      
        1 gtagttgatg atgttatcga ctttatgctc ggtcgcttcc gcgcctggta tcaggacgaa
       61 ggttataccg ttgacaccat ccaggcggta ctggcgcgtc gtccgactcg tccggctgat
      121 ttcgatgccc gcatgaaagc ggtatcgcac ttccgtaccc tggaagcagc tgcagcactg
      181 gcggcggcga acaaacgtgt atctaacatc ctggcgaaat ctgacgaagt gctgagcgat
      241 cgcgtgaatg cctctaccct gaaagagccg gaagaaatta aactggcgat gcaggttgtg
      301 gtgctacgtg acaagctgga gccgtacttt gcggaaggtc gttaccagga tgcgctggtc
      361 gaactggcag agctgcgtga gccggttgat gctttcttcg ataaagtgat ggtcatggtt
      421 gatgacaaag aattgcgtct caaccgtctg accatgctgg agaaactgcg cgaactgttc
      481 ctgcgcgttg cggatatttc gctgttgcag taataacgcc gttattaaaa agcctgccat
      541 ctggcagg
//
LOCUS       DAJVPT010000368          544 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVPT010000368 DAJVPT010000000
VERSION     DAJVPT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            6..>544
                     /locus_tag="OQM47_004273"
     CDS             6..>544
                     /locus_tag="OQM47_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1482158.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKL"
BASE COUNT          112 a          157 c          158 g          117 t
ORIGIN      
        1 tcctcatgtt gtccgctttt actccccgcc cgctgaaacg tctgttcacg gccaaccagt
       61 gctggacatc cttcctggat gcgggcggtc tgcgcgatat cgaggttgag gctgtcacaa
      121 aaatgctggc ctgcggcacg cggatactgg gcgtgaagga atacatctgc gataaacctg
      181 agtgccccca cgtcagatac gtcactaact catgcggcag ccgtgcctgc ccgtcctgcg
      241 gaaaaaaggc cacagacctg tggatagcga cacagctgaa tcgtcttcct gactgcgact
      301 gggtacacct ggtcttcacc ctgccggaca cgcagtggcc ggtgttcgaa agcaaccggt
      361 ggctgctgaa tgacgtgtgc cgtctggcgg tggagaatct gctgtatgcc gcccggaaac
      421 gggggcagga acccggtatc ttctgcgcca tccacacgta tggccgtcgt ctcaactggc
      481 atccgcatgt acatgtgtct gtaacctgtg gaggtctgaa taagcatggt cagtggaaaa
      541 agct
//
LOCUS       DAJVPT010000369          534 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVPT010000369 DAJVPT010000000
VERSION     DAJVPT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>534)
                     /locus_tag="OQM47_004274"
     CDS             complement(<1..>534)
                     /locus_tag="OQM47_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCS1482159.1"
                     /translation="HRLAGFFVRLVVACAFAAQAPLSSADLYFNLRFLADDPQAVADL
                     SRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTA
                     SVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRARGYIPPELWDP
                     GINAGLLNYNFSGNSVQN"
BASE COUNT          156 a          139 c          126 g          113 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatg
//
LOCUS       DAJVPT010000370          521 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVPT010000370 DAJVPT010000000
VERSION     DAJVPT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>521)
                     /locus_tag="OQM47_004275"
     CDS             complement(<1..>521)
                     /locus_tag="OQM47_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1482160.1"
                     /translation="QRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLR
                     MKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPT
                     SVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAID
                     DFGTGYASYARLK"
BASE COUNT          141 a          130 c          118 g          132 t
ORIGIN      
        1 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
       61 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      121 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      181 gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      241 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      301 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc taccggggcc
      361 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      421 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      481 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc a
//
LOCUS       DAJVPT010000371          515 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVPT010000371 DAJVPT010000000
VERSION     DAJVPT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..460
                     /locus_tag="OQM47_004276"
     CDS             <1..460
                     /locus_tag="OQM47_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1482161.1"
                     /translation="LYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAA
                     QLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQC
                     PVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS"
BASE COUNT          108 a          139 c          148 g          120 t
ORIGIN      
        1 gctttatctt ggcgcgctgc cggagctttc tgtcagcttc ctgcgtgaac aaggtttccg
       61 tactgcttgg aaagatgatc cgcgcgaagt gcatttcctg tatgccaaca gctatgagcg
      121 ggaggcggct gcccagttat tcgaaaaatg gctggaaacg catccgatgc cgcaggcgct
      181 gttcacaacg tcgtttgcgt tgttgcaagg agtgatggat gtcacgctgc gtcgcgacgg
      241 caaactgcct tctgacctgg caattgccac ctttggcgat aacgaactgc tcgacttctt
      301 acagtgtccg gtgctggcag tggctcaacg tcaccgcgat gtcgcagagc gtgtgctgga
      361 gattgtcctg gcaagcctgg acgaaccgcg taagccaaaa cctggtttaa cgcgcattaa
      421 acgtaatctc tatcgccgcg gcgtgctcag ccgtagctaa tccgcgaaca aaaatacgcg
      481 ccaggtgaat ttccctctgg cgcgtagagt acggg
//
LOCUS       DAJVPT010000372          515 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVPT010000372 DAJVPT010000000
VERSION     DAJVPT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>515
                     /locus_tag="OQM47_004277"
     CDS             <1..>515
                     /locus_tag="OQM47_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1482162.1"
                     /translation="VSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFA
                     GMQLRDITESKIYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATH
                     LSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVE
                     FALATGLRRSN"
BASE COUNT          180 a          107 c          126 g          102 t
ORIGIN      
        1 ggtcagcaaa cttggtgaaa tacctgatat aacgttcgag gaagcgtgtg tcaggtggct
       61 tgaagagaaa gcacataaaa aatcactgga cgatgacaaa agccggatcg gattctggct
      121 tcaacatttc gcaggaatgc aactaagaga cattactgaa tcaaaaattt attcagcaat
      181 gcagaaaatg acgaaccggc gtcatgagga aaactggaga ctcagggcag aagcatgcag
      241 aaaaaaaggg aaacctgttc cagaatacac gccaaaacca gcgtccgttg caacgaaggc
      301 tacgcatctt tcatttataa aggccctact aagagccgca gagcgtgaat ggaaaatgct
      361 ggataaggca ccaattatta aagtgcctca accaaagaat aaacggatcc gctggctgga
      421 gccccatgaa gcacaaaggc tgattgatga atgtccggag ccattaaagt ctgttgttga
      481 atttgcactg gcaacaggct taagacgctc gaaca
//
LOCUS       DAJVPT010000373          513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVPT010000373 DAJVPT010000000
VERSION     DAJVPT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>513
                     /locus_tag="OQM47_004278"
     CDS             <1..>513
                     /locus_tag="OQM47_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1482163.1"
                     /translation="VNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITL
                     HQNNADTTGTQEKITKDKDIVFTNGGNVLFKDNLDFGCGGIIFDEGHEYNINGQRFTF
                     KGAGIDIGKESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTF
                     NNIYLASGNGK"
BASE COUNT          209 a           66 c          104 g          134 t
ORIGIN      
        1 gttaatggtg accaactgac atggataaca aaatacaatg ataaactggt tagtgagtta
       61 aaagatacct atagtcataa aataaatctg aatggcaata atgtaaccat taaaaacaca
      121 gatataacat tacaccaaaa caatgcagat accactggta ctcaagaaaa aataactaaa
      181 gacaaagata ttgtgttcac aaatggggga aatgttctgt ttaaggataa tttggatttt
      241 ggttgcggtg gtattatctt tgacgaaggc catgaatata acataaacgg tcagagattt
      301 acatttaaag gagcaggaat tgatatcgga aaagaaagca ttgtaaactg gaatgcattg
      361 tattccagtg atgatgtttt acacaaaata ggccccggta ctctgaatgt tcaaaaaaaa
      421 cagggggcaa atataaagat aggtgaagga aatgttattc ttaatgaaga aggaacattt
      481 aacaatatat accttgcaag cggaaatggt aag
//
LOCUS       DAJVPT010000374          512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVPT010000374 DAJVPT010000000
VERSION     DAJVPT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..355
                     /locus_tag="OQM47_004279"
     CDS             <1..355
                     /locus_tag="OQM47_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCS1482164.1"
                     /translation="YACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQ
                     CRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGR
                     PASDIMQAHLMVVNK"
BASE COUNT          124 a          117 c          141 g          130 t
ORIGIN      
        1 ttacgcctgc gatcactatg tttatccgca atatcggctg gggaatatgc atcagcaaac
       61 aattgcagaa atgatcgatt ccccgcaaca gcaggtgttt ggtgaagata aatttaagca
      121 attaccggcg cagtgtcgca gttgtaacgt gttaaaagca tgttggggag gctgcccgaa
      181 acaccgcttc atgctcgatg ccagcggcaa accggggctg aattatttgt gtgccgggta
      241 tcagcgttat ttccgccatc taccgccata tcttaaagca atggctgatt tgctggcgca
      301 cggtcgcccg gccagtgaca ttatgcaggc acatttgatg gtggtgaata agtaaaaaag
      361 cggccatttg gaagccgcct gcggttgatt gccggatgcg gcgtaaacgc cttatccggc
      421 ctacatgatc gtgcaaattc aataaattgc agcgttctgt ggatactggt ttgggtgatc
      481 gtatccggca aagcctttac gtgcggtggc ag
//
LOCUS       DAJVPT010000375          511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVPT010000375 DAJVPT010000000
VERSION     DAJVPT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..511
                     /locus_tag="OQM47_004280"
                     /pseudo
     CDS             1..511
                     /locus_tag="OQM47_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJVPT010000376          508 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVPT010000376 DAJVPT010000000
VERSION     DAJVPT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..139
                     /locus_tag="OQM47_004281"
     CDS             <1..139
                     /locus_tag="OQM47_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482165.1"
                     /translation="AGDPGRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDKTR
                     E"
     gene            136..>508
                     /locus_tag="OQM47_004282"
     CDS             136..>508
                     /locus_tag="OQM47_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1482166.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPL"
BASE COUNT          153 a          131 c          135 g           89 t
ORIGIN      
        1 ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc tcagttatcc atgctactgg
       61 aggggatcgc gtggcaacat cccaaacgga cagaacggcc tggcatccgg atataacccg
      121 tgataaaaca agggaatgaa caatgaactc cccgatgata ttgagctgct taaagccatg
      181 ttgcgtaagc aacagagtcg gcttcgacag tatgcctgtc aggtcgcggg ctatgagcag
      241 gaaattgaac ggctgaaagc gcaactcgac aggttgcgtc gtatgttgtt cggccagagt
      301 tcagagaaaa agcgtcataa gcttgaaaat cagatccgac aggcagaaaa acgactgtcg
      361 gaactggaaa accggctgaa cacagccaga aatcttctgg aagatgcatc gtcagtcaca
      421 gattcacctg acaccagtcc cccgtcagaa aacccgatcg ccagtaagcc tgaatccccg
      481 ggacgaaaat cttcacgaaa accgctgc
//
LOCUS       DAJVPT010000377          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVPT010000377 DAJVPT010000000
VERSION     DAJVPT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..312)
                     /locus_tag="OQM47_004283"
     CDS             complement(1..312)
                     /locus_tag="OQM47_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1482167.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT          106 a          141 c          134 g          125 t
ORIGIN      
        1 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
       61 ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg aaattgtgcc
      121 atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac gctttaacac
      181 accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat caccgtaagt
      241 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
      301 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
      361 gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca attagccggg
      421 gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg gctctgttgg
      481 ttctcccgca atgctactct gtttac
//
LOCUS       DAJVPT010000378          505 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVPT010000378 DAJVPT010000000
VERSION     DAJVPT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            142..315
                     /locus_tag="OQM47_004284"
     CDS             142..315
                     /locus_tag="OQM47_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482168.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(387..>505)
                     /gene="hokB"
                     /locus_tag="OQM47_004285"
     CDS             complement(387..>505)
                     /gene="hokB"
                     /locus_tag="OQM47_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1482169.1"
                     /translation="IICITILAFTLLTRQTLYELRFRDGDKEVAALMACTSR"
BASE COUNT          143 a          114 c          117 g          131 t
ORIGIN      
        1 aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt cacccgatct
       61 ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg cacataagat
      121 tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat gggaacgctt
      181 gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca
      241 cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc atcgaaaaaa
      301 acatgcacta actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc tagtcagatg
      361 cgcggggaaa atccccgcgc ttgcccttac ctggacgtgc aggccatgag cgcagcaacc
      421 tccttatcac cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag gagtgtgaat
      481 gccagaatcg taatgcagat aatga
//
LOCUS       DAJVPT010000379          500 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVPT010000379 DAJVPT010000000
VERSION     DAJVPT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>500
                     /locus_tag="OQM47_004286"
     CDS             <1..>500
                     /locus_tag="OQM47_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1482170.1"
                     /translation="GRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATL
                     DRLSNGRALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGK
                     HIHVRGAKLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQV
                     RAKAAA"
BASE COUNT          102 a          143 c          146 g          109 t
ORIGIN      
        1 cggggcgatc gtgtgaagat gcgtggctgg ttgcagcgtc gatgatcccg gtgacgcagc
       61 ggctgaagtt tcttgtcgcc ctgcgtccca gcgtaacctc acctactgtt gccgcccgcc
      121 aggccgccac gcttgaccgt ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca
      181 gcgatccaca agagctggca ggcgacggcg tgttccttga tcatagcgag cgctacgaag
      241 cctcggcgga atttacccag gtctggcggc gcttattgct gggggaaacc gtcaatttca
      301 acggtaaaca tattcatgtg cgcggagcaa aactgctctt cccgccgatt caacagcctt
      361 atcctccgct ttactttggc ggatcgtcag atgtcgccca ggagctggcg gcagaacaag
      421 ttgatctcta cctcacctgg ggcgaaccgc cggaacttgt taaagagaaa atcgaacaag
      481 tgcgggcgaa agctgccgcg
//
LOCUS       DAJVPT010000380          500 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVPT010000380 DAJVPT010000000
VERSION     DAJVPT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..200
                     /locus_tag="OQM47_004287"
     CDS             <1..200
                     /locus_tag="OQM47_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCS1482171.1"
                     /translation="VDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRT
                     RKKSFGWYAEVIKTRGLSLKK"
     gene            286..>500
                     /locus_tag="OQM47_004288"
     CDS             286..>500
                     /locus_tag="OQM47_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCS1482172.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTN"
BASE COUNT          169 a           88 c          113 g          130 t
ORIGIN      
        1 gtgtggatat tatggggtac accagttggg ggccaattga tttagtcagt gcatctcatt
       61 cacaaatgtc taagcgctac ggctttattt atgtggatcg tgatgataat ggcgaaggaa
      121 gcctcacaag aacgcgtaag aaaagcttcg gatggtatgc agaagtgatc aaaacgcggg
      181 ggctgtcatt aaaaaaataa ccaataaagc accttaatta tcgtcgcatt cagaacagtc
      241 tggatgcgat acgttaattc tttctttgca ccataaaggg atattatgtt tagacaaaat
      301 cttattacct ctgccatctt attaatggca ccgttagcct tttctgcaca atcattggct
      361 gaatcattaa cggtggaaca acgccttgag ttattagaaa aagcgttaag agaaacgcaa
      421 agcgaactca aaaagtataa agatgaagag aagaaaaaat atacgccagc gacggtgaat
      481 cgtagcgtaa gtacgaatga
//
LOCUS       DAJVPT010000381          499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVPT010000381 DAJVPT010000000
VERSION     DAJVPT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          187 a           92 c           73 g          147 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatg
//
LOCUS       DAJVPT010000382          498 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVPT010000382 DAJVPT010000000
VERSION     DAJVPT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>498
                     /gene="rlmB"
                     /locus_tag="OQM47_004289"
     CDS             <1..>498
                     /gene="rlmB"
                     /locus_tag="OQM47_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1482173.1"
                     /translation="GIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALESQGVVI
                     QLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHN
                     LGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQE
                     ENIWIV"
BASE COUNT          110 a          142 c          143 g          103 t
ORIGIN      
        1 cggcatccac gcagtgcagg ccctgctgga gcgcgcccct gaacgttttc aggaagtctt
       61 tattttaaaa ggccgtgaag ataaacgtct gttaccgctg attcacgccc ttgagtctca
      121 gggcgtggtt atccagttgg caaaccgcca atatctcgac gagaaaagcg acggtgccgt
      181 gcatcagggc attatcgccc gcgtgaagcc aggacgtcag tatcaggaaa acgatctgcc
      241 ggatctgatc gcttcgctcg atcaaccgtt cctgctgatc ctcgacggcg taaccgatcc
      301 gcacaacctc ggcgcgtgcc tgcgtagtgc ggacgccgca ggcgttcatg cggtgattgt
      361 gccgaaagat cgctccgcac agctcaacgc tacggcgaaa aaagtagcct gcggcgcggc
      421 agaaagcgtt ccgctgattc gggtgactaa ccttgcgcgc accatgcgta tgttgcagga
      481 agagaatatc tggatcgt
//
LOCUS       DAJVPT010000383          493 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVPT010000383 DAJVPT010000000
VERSION     DAJVPT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            195..290
                     /gene="mgtS"
                     /locus_tag="OQM47_004290"
     CDS             195..290
                     /gene="mgtS"
                     /locus_tag="OQM47_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCS1482174.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT          135 a           95 c          116 g          147 t
ORIGIN      
        1 ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag accgtggggg cagcccgcgc
       61 tttgttgatt taagtcgaac acaataaaaa tttaattcag ccttcgttta ggttatctct
      121 gctaatatct ttctcattga gatgaaaatt aaggtaagcg aggaaacaca ccacaccata
      181 aacggaggca aataatgctg ggtaatatga atgtttttat ggccgtactg ggaataattt
      241 tattttctgg ttttctggcc gcgtatttca gccacaaatg ggatgactaa tgaacggaga
      301 taatccctca cctaaccggc cccttgttac agttgtgtac aaggggcctg atttttatga
      361 cggcgaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt
      421 gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac agtagcatca
      481 gttttctcaa tga
//
LOCUS       DAJVPT010000384          492 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVPT010000384 DAJVPT010000000
VERSION     DAJVPT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          136 a          112 c          117 g          127 t
ORIGIN      
        1 caaagagtgt cttgaggtaa gagacgctat tgctcacggc gattcgtata cttcgagctt
       61 taatttctcg gcggatcatt tggataacca agacgatatt gcaaaagtgt cctggcggta
      121 aactctgaac aatttcgggg attaactgtt gagcgccttg caaaatacat tgaaatggca
      181 aagcatattg accatgagat ttctgataat ggttacaggc cttctgaagg ttaccttcca
      241 gcagccgagc gcagaagtct gatgttagct tttggcgtca gtggtgtgtc aatgtggccc
      301 agtgcttgag cgataataca cctcactata gaggaatgga cccttaacta tcactatagg
      361 ggagagaccc taagtcccca ccttaatagc agtcacgacc catacacaca ttcattaagt
      421 ctcaggcttc cggtagcgga ccgattggaa accatccgca gacgcctagt gttaaatcac
      481 cttggcctga ga
//
LOCUS       DAJVPT010000385          489 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVPT010000385 DAJVPT010000000
VERSION     DAJVPT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..>489
                     /locus_tag="OQM47_004291"
     CDS             1..>489
                     /locus_tag="OQM47_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1482175.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSK"
BASE COUNT          132 a          104 c          117 g          136 t
ORIGIN      
        1 gtgtttaatt ctgacaacct gagactcgac ggaaaatgcg ccatcatcac aggtgcgggt
       61 gcaggtattg gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc tgtggtggtc
      121 agtgatatta acgccgacgc agctaaccat gttgtagacg aaattcaaca actggatggt
      181 caggcatttg cctggcgttg tgatattact tccgaacagg aactctctgc actggcagac
      241 tttgccgtca gtaatctggg taaagttgat atcctggtta acaacgccgg tggcggtggt
      301 cctaaaccgt ttgatatgcc aatggcagat tttcgccgtg cttatgaact gaatgtgttt
      361 tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg aggtggcgtt
      421 attctgacca tcacttctat ggcggcagaa aataaaaata taaacatgac ttcctatgca
      481 tcatctaaa
//
LOCUS       DAJVPT010000386          487 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVPT010000386 DAJVPT010000000
VERSION     DAJVPT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(273..>487)
                     /gene="rpsT"
                     /locus_tag="OQM47_004292"
     CDS             complement(273..>487)
                     /gene="rpsT"
                     /locus_tag="OQM47_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1482176.1"
                     /translation="RKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQ
                     AAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT          117 a          102 c          117 g          151 t
ORIGIN      
        1 atagagtggt cttaagttta atacctcaat gtgtatcaca atatccatat tctttgtggg
       61 ggagtctgga gattgagtag atattcttgt tcagaatgta tcagccgatg gttctacgat
      121 tcttaagcca cgaagagttc agatagtaca acggcatgtc tcttttgact atctggcaac
      181 cggcagtgtg ttctctcacg catcacaaaa gcagcaggca taaaaaaacc cgcttgcgcg
      241 ggctttttca caaagcttca gcaaattggc gattaagcca gtttgttgat ctgtgcagtc
      301 aggttagcct tatgacgtgc agctttgttt ttgtggatca gacctttagc agcctgacgg
      361 tccacgatcg gttgcatttc gttaaatgct ttctgtgcag cagctttgtc gccagcttcg
      421 atagctgcgt atactttctt gatgaaagta cgcatcatag agcgacggct tgcgttgtgc
      481 ttacgag
//
LOCUS       DAJVPT010000387          483 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVPT010000387 DAJVPT010000000
VERSION     DAJVPT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>483)
                     /gene="ospC4"
                     /locus_tag="OQM47_004293"
     CDS             complement(<1..>483)
                     /gene="ospC4"
                     /locus_tag="OQM47_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1482177.1"
                     /translation="DHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGAR
                     SSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNIIL
                     NIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFG
                     A"
BASE COUNT          157 a           66 c           94 g          166 t
ORIGIN      
        1 gcgccaaaat ctacagtatg atgtgtttta tttagtggga gtttttcttg atgattggaa
       61 atttcgaccc caaaaaaaac aaaatcgtgg ttagagattt ctttaatatc ctcagagtat
      121 gtattcttat gtgtttgtat attttcagac tgaattttat caaaggattt aatattaaga
      181 ataatatttg acagattaga gttactttga tgtctgaagt taaaattaga attaataata
      241 atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat tatctcagag
      301 atatagtgcc tggacacctt atcgagatgc tcaagtgacc gaaatgatga ccgtgctcca
      361 gatggcgctt caagagaaag atttagtgat ttgaaactgg tcccttgaga aaacattttg
      421 ctataacttt gcgctgctat agacttcctt aaaaagtttt tcacagtatt agcacagtga
      481 tct
//
LOCUS       DAJVPT010000388          479 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVPT010000388 DAJVPT010000000
VERSION     DAJVPT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>479)
                     /gene="ospC2"
                     /locus_tag="OQM47_004294"
     CDS             complement(<1..>479)
                     /gene="ospC2"
                     /locus_tag="OQM47_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1482178.1"
                     /translation="YSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSE
                     IIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNT
                     FSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLT"
BASE COUNT          157 a           67 c           94 g          161 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactata
//
LOCUS       DAJVPT010000389          478 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVPT010000389 DAJVPT010000000
VERSION     DAJVPT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(235..>478)
                     /locus_tag="OQM47_004295"
     CDS             complement(235..>478)
                     /locus_tag="OQM47_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482179.1"
                     /translation="EAARLDARSIATLFELCERLQMQLIIAAPENISPEKGTTYKLVR
                     KVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
BASE COUNT          122 a          113 c          122 g          121 t
ORIGIN      
        1 ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg
       61 tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac
      121 tggatcggtc aactgatcct taactgatcg gcattacgcc gttatgtgcc atttttttag
      181 tgcagaaacg gagttttcgg aaaaagaaaa ggcggcattg ctgccgcctt aattttaact
      241 cgcctgggaa ggcgcttcgt ctcttcctag aagcgtttca gggagttgcg gcgcaaatcc
      301 tcgcaggccg acgacgtgaa catgttcggt attctggaag actttacgta ccagtttata
      361 ggtggtgcct ttttccggac tgatattttc cggcgctgcg atgatgagct gcatttgcaa
      421 acgttcacac aattcaaaca gcgtggcgat agaacgagca tccagtcgcg ctgcttca
//
LOCUS       DAJVPT010000390          478 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVPT010000390 DAJVPT010000000
VERSION     DAJVPT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..253)
                     /locus_tag="OQM47_004296"
     CDS             complement(<1..253)
                     /locus_tag="OQM47_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HCS1482180.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVS"
     gene            complement(307..>478)
                     /gene="arsR"
                     /locus_tag="OQM47_004297"
     CDS             complement(307..>478)
                     /gene="arsR"
                     /locus_tag="OQM47_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1482181.1"
                     /translation="DRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLAR
                     QNCSGDSKNICS"
BASE COUNT          127 a          139 c          103 g          109 t
ORIGIN      
        1 gtgaaacgtg cagcgctgcc cattcaaaaa agccggactc atccagcagc aggctgatga
       61 taatgacggc aataaacgcc gccgtcgcgt tccagacgat attccacacc accggaatat
      121 cacccggatg aaccacgccc gtaaccaacg ccagtaccgc gccgagcgtt gcactccagc
      181 cgatgcctaa acctttcggc tgccagataa ccaatacgat ggtcaggaca aagatagcgc
      241 ctgccagtaa cataatgcct cccggataaa acacatgtgc aaatccatat gtgtttagct
      301 aaatttttaa ctgcaaatgt tcttactgtc tccggaacag ttttgtctgg ccaggttgcg
      361 gacaattgcc tgaatctttt cctgttcaca tcgccaggct tcatcaataa ttttcgccgc
      421 ccatgctgga atatgcggcg ataagcggta atgaacccac ttaccttgct tgcggtcc
//
LOCUS       DAJVPT010000391          470 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVPT010000391 DAJVPT010000000
VERSION     DAJVPT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            163..>470
                     /locus_tag="OQM47_004298"
     CDS             163..>470
                     /locus_tag="OQM47_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482182.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     V"
BASE COUNT          114 a          107 c          116 g          133 t
ORIGIN      
        1 gcctgtttac gttgatgacg tgccagtttt tcttcatgaa gcagatgctc caggaagtcc
       61 atataactcc attcctggtc tactgcctgt tgtgacagcg caggcgctgc gcttatgtac
      121 tgcacccatt ttgttggacg atgaaatgga atagcccctg atatgtcaaa gccaaaatac
      181 ccttttgaaa agcgccttga agtcgtgaat cactacttca ctactgatga tggttacagg
      241 atcatctcgg cacgttttgg tgtcccccga acccaggtca ggacatgggt tgccctctat
      301 gaaaaacatg gagaaaaagg cttaattccc aaacctaaag gtgttagtgc tgatccagag
      361 ttgcgtatta aggtcgtgaa agctgtgatt gagcagcaca tgtcccttaa tcaggctgct
      421 gctcacttta tgcttgctgg tagtggttct gtagccaggt ggctgaaggt
//
LOCUS       DAJVPT010000392          463 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVPT010000392 DAJVPT010000000
VERSION     DAJVPT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>463
                     /locus_tag="OQM47_004299"
     CDS             <1..>463
                     /locus_tag="OQM47_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1482183.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJVPT010000393          462 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVPT010000393 DAJVPT010000000
VERSION     DAJVPT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            195..462
                     /locus_tag="OQM47_004300"
                     /pseudo
     CDS             195..462
                     /locus_tag="OQM47_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT          114 a          125 c          125 g           98 t
ORIGIN      
        1 tgcgcatgaa gacggaacta gtccagatgt gatatttgtg gtgatcacat acatcatcaa
       61 cgatgctcgt tatggtgagt ttgatgacta cccgctgaag tgaaaatagt gttgtgtacc
      121 aaattgcgta ccaaactaaa atcacaaatc atgaaaccct tgttcatggc ggttctcagg
      181 gggattgcgc gtaatcgcga attcagggta tggacccggt gtgggtcact gctgcgatcc
      241 cggagtccat cgcctggctg gtgaaagatg cctcgcagtt taccctcacc gttccggcgc
      301 gaccggataa aacactcacc atccgcaaat ggacgctgct acctggcgtg gatgccgcga
      361 cccgcacgct gcagctgcgt ctggaagtcg acaacgccga cgaggcgcta aaaccgggca
      421 tgaacgcctg gttgcaactc aacaccgcca gcgaaccgat gc
//
LOCUS       DAJVPT010000394          456 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVPT010000394 DAJVPT010000000
VERSION     DAJVPT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            333..>456
                     /locus_tag="OQM47_004301"
     CDS             333..>456
                     /locus_tag="OQM47_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1482184.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGV"
BASE COUNT          139 a           92 c          102 g          123 t
ORIGIN      
        1 aggaaacagt atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac
       61 gaactgatgg caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata
      121 aggcaagccg aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg
      181 gcaattatcg catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg
      241 gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat ttatgccatt
      301 tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct gctttagccc
      361 ttgttctgct gaccaccgga cttgtcggtt gcgatcgcgt tgagcctggt aatgttggca
      421 tcaaggtaaa caaactgggc gacgataaag gcgtcg
//
LOCUS       DAJVPT010000395          451 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVPT010000395 DAJVPT010000000
VERSION     DAJVPT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>451
                     /gene="ssuE"
                     /locus_tag="OQM47_004302"
     CDS             <1..>451
                     /gene="ssuE"
                     /locus_tag="OQM47_004302"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1482185.1"
                     /translation="SRSSSLLEYAREKLNGLDVEVYHWNLQNFAPEDLLYARFDSPAL
                     KTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLL
                     AVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDT"
BASE COUNT          105 a          131 c          110 g          105 t
ORIGIN      
        1 cttctcgctc cagctccttg ctggaatatg cgcgggaaaa actaaatggc ctagatgtag
       61 aggtttatca ctggaatctg caaaacttcg ccccggaaga tctgctttat gctcgtttcg
      121 atagtccggc actcaagacc ttcaccgaac agctgcaaca ggctgatggg ctgattgtcg
      181 ccacgcctgt gtataaagcc gcctattccg gcgcgctgaa aaccctgctc gacctgctgc
      241 cagagcgcgc tttgcaaggc aaagtggtgc taccgctggc gacgggcggt accgtggccc
      301 atctgctggc ggtcgattat gcccttaaac cagttttaag cgcactgaaa gctcaggaga
      361 tcctgcacgg cgtgtttgcc gatgactcac aattaattga ttaccatcac agaccccagt
      421 tcacgccaaa tctgcaaacc catcttgata c
//
LOCUS       DAJVPT010000396          449 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVPT010000396 DAJVPT010000000
VERSION     DAJVPT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..233
                     /locus_tag="OQM47_004303"
     CDS             <1..233
                     /locus_tag="OQM47_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS1482186.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(315..449)
                     /locus_tag="OQM47_004304"
                     /pseudo
     CDS             complement(315..449)
                     /locus_tag="OQM47_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          104 a          100 c          116 g          129 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat ttgtgatgtc agatgtcccc tgtaaaacta tgttttcatt gcgctctatg
      361 aagctgagtg agcgtaacga ctggagctgc ttctgcggtg ctccggtggc gaacgtgaag
      421 tcatactctt cgaacgtttt caccgccgg
//
LOCUS       DAJVPT010000397          447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVPT010000397 DAJVPT010000000
VERSION     DAJVPT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(241..>447)
                     /locus_tag="OQM47_004305"
     CDS             complement(241..>447)
                     /locus_tag="OQM47_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1482187.1"
                     /translation="VKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMV
                     YEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          119 a           85 c           97 g          146 t
ORIGIN      
        1 atgaggaaaa ttgttgatgg caaaaatggg gcaaaaaaga gtgggttatc gtagcttatt
       61 gttgtcgctg ataatgttta acatattgaa aaataaataa aatacttatg agtcagatag
      121 ttgtgatttt tgcccttact tgttcaggtt gtattgttct ttctttgtaa tttgttgatt
      181 ttcttgcatt atttcagttc tctggtacta aatggggcaa attgggggca aactttgcaa
      241 ctacgataac cgcgcattca acatggctat ctgttcgtcg ttcatgtcat caatccacat
      301 accgtaaatt tcatacacca tttgcgcagt ttcatgcccc atctggctgg ctataaatgc
      361 cgggttcgct cctgccgtca acagccagca ggcaaaagta tgtcgcgtat ggtacggatt
      421 acggcggcga ataccagcac gttttac
//
LOCUS       DAJVPT010000398          447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVPT010000398 DAJVPT010000000
VERSION     DAJVPT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            35..447
                     /locus_tag="OQM47_004306"
                     /pseudo
     CDS             35..447
                     /locus_tag="OQM47_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          120 g          111 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJVPT010000399          445 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVPT010000399 DAJVPT010000000
VERSION     DAJVPT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>445
                     /locus_tag="OQM47_004307"
     CDS             <1..>445
                     /locus_tag="OQM47_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482188.1"
                     /translation="QDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGK
                     ACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRY
                     VDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKRHKNGVRIP"
BASE COUNT          116 a           92 c          130 g          107 t
ORIGIN      
        1 caggacacta tcaacgcttt ctacggcaag cagcagtatc tgtttaatcc accgtgggaa
       61 tctctggcag ataaattcca gttccgtgag gcagagttga cgctggtcaa tggtgtgcac
      121 ggtcacggaa aggcatgccc actgaatgag cctattcttt tagctgatgg gacatggact
      181 actcacggga atgtaaaaat tggcgatcag gtggcgtcag tagacggcaa tccgtcaact
      241 gtcactggga tattcccgca gggtgttaga gatgtttacc gagtcacatt tgaagatggt
      301 cgttatgttg attgcgcagg cgatcaccta tgggaggtca ctagtcgtgg attcacgaaa
      361 ggcgagaaac gccgcgtgat agataccttc gggttgaagc ggttgagtga aacgaagagg
      421 cacaaaaatg gcgttaggat tcctg
//
LOCUS       DAJVPT010000400          439 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVPT010000400 DAJVPT010000000
VERSION     DAJVPT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..269
                     /locus_tag="OQM47_004308"
     CDS             <1..269
                     /locus_tag="OQM47_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482189.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="OQM47_004309"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="OQM47_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1482190.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DAJVPT010000401          439 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVPT010000401 DAJVPT010000000
VERSION     DAJVPT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>439
                     /locus_tag="OQM47_004310"
     CDS             <1..>439
                     /locus_tag="OQM47_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1482191.1"
                     /translation="SLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIK
                     SRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSD
                     QLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNLDPIEKR"
BASE COUNT          131 a           89 c          117 g          102 t
ORIGIN      
        1 atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg ctgaaaggct
       61 taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca aggcagttga
      121 gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg aagcaatcag
      181 gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa aagatgctct
      241 tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc tgatgaaacg
      301 aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa aaattgatgc
      361 gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga tgaaaaacct
      421 tgaccctatt gaaaagaga
//
LOCUS       DAJVPT010000402          436 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVPT010000402 DAJVPT010000000
VERSION     DAJVPT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            210..>436
                     /locus_tag="OQM47_004311"
     CDS             210..>436
                     /locus_tag="OQM47_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1482192.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERG"
BASE COUNT          121 a           87 c           97 g          131 t
ORIGIN      
        1 tccttgccaa caatcttgca aaaaaaatgc gattgctaaa acaaaagtgc aatttgcggc
       61 aatatagtta ttccatgacc gtacaatctt gcattcagtg taaataatct tgtttagctc
      121 tcgctaaacg cggttgtata accgcaatac acgccgtatt ttatccggca tatattgttt
      181 ttgcttgctc atggaatagg gaggttgtca tgtatcaacg ctgttttgat aatgccagtg
      241 aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg ttttgcattt agcgatgatc
      301 caaaatggga gtctggacat cacgttcatg acaatgaaac cgagctgatt tacgtcaaga
      361 aaggggttgc aaggtttacc atcgattctt cgttatatgt cgcacatgcg gatgacattg
      421 tggtgataga acgcgg
//
LOCUS       DAJVPT010000403          435 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVPT010000403 DAJVPT010000000
VERSION     DAJVPT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>435
                     /locus_tag="OQM47_004312"
     CDS             <1..>435
                     /locus_tag="OQM47_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482193.1"
                     /translation="GLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAE
                     GVAMFNARTGRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRK
                     GEFTLKVGGSLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHST"
BASE COUNT          124 a           94 c          122 g           95 t
ORIGIN      
        1 gggcttgagg aatacgttga taagcatccg gcactggctg gcgcatatac ggggccaaat
       61 ccgcaggcaa aacctgataa ctatggcgac cacgctgttg atgcagagct gtttctgaaa
      121 acctttgccg aaggtgtggc gatgttcaat gccagaacag gccgtgaaac agaaatgtgc
      181 gggggcaaac tctcgtttga tgatgttttc gagcgtgaat acgccagaac gattgtgcgt
      241 aagccaaccg aagaacaaaa acggatgctg ttactgcctg ccgaggcggt gaacgtttca
      301 cgcaaaggcg agtttacgct taaagttggc ggctccctta aaggcgcgaa aaacgtttat
      361 tacaacatgg cattaatgaa tgccggagtg aaaaaagttg tggtcagatt tgatccacag
      421 cagttacaca gcacg
//
LOCUS       DAJVPT010000404          434 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVPT010000404 DAJVPT010000000
VERSION     DAJVPT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..131
                     /locus_tag="OQM47_004313"
     CDS             <1..131
                     /locus_tag="OQM47_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1482194.1"
                     /translation="HFDAQALTELTALIGLQNLSARFNSAMDIPAQGLCRIPEKRS"
     gene            141..>434
                     /locus_tag="OQM47_004314"
     CDS             141..>434
                     /locus_tag="OQM47_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1482195.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLW"
BASE COUNT           97 a          107 c          128 g          102 t
ORIGIN      
        1 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
       61 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
      121 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
      181 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
      241 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
      301 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
      361 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg
      421 tggatctgct ctgg
//
LOCUS       DAJVPT010000405          428 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVPT010000405 DAJVPT010000000
VERSION     DAJVPT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..295)
                     /locus_tag="OQM47_004315"
                     /pseudo
     CDS             complement(1..295)
                     /locus_tag="OQM47_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            299..428
                     /locus_tag="OQM47_004316"
                     /pseudo
     CDS             299..428
                     /locus_tag="OQM47_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a          121 c          100 g          107 t
ORIGIN      
        1 gctgcgctat tttcagctca ggttcttttt cacacatcag actaataaat cgggaagcat
       61 aattttcttc atccctggtt attctccagg gcatcaacca gcggcttacc cgggagactg
      121 aaggaagtct gacggcagta gtaaccggtg ggttccagcc tttacgccat ttggcaaccg
      181 catccctgac gatggtttca ctgcctgtaa atccccgggc caccatttcc cgccatatcc
      241 ggctggcgtt ataattacca ctttctcgtt gctcttttaa ccactccctc catggtgtca
      301 gcgaaaaacg aaaccgctgt tgaaatccct ggaaagctgg ttgcggaaaa gatgaagacc
      361 cagtcgcgac actcagagtt ggcgaaggcg ttcgcgtacg cacttaacca gtggccggca
      421 ctgacgta
//
LOCUS       DAJVPT010000406          428 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVPT010000406 DAJVPT010000000
VERSION     DAJVPT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="OQM47_004317"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="OQM47_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCS1482196.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAJVPT010000407          426 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVPT010000407 DAJVPT010000000
VERSION     DAJVPT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.413"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            1..426
                     /locus_tag="OQM47_004318"
                     /pseudo
     CDS             1..426
                     /locus_tag="OQM47_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DAJVPT010000408          422 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVPT010000408 DAJVPT010000000
VERSION     DAJVPT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>422
                     /locus_tag="OQM47_004319"
     CDS             <1..>422
                     /locus_tag="OQM47_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1482197.1"
                     /translation="PLFNSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNT
                     WPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMAD
                     KVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPG"
BASE COUNT          113 a           97 c          105 g          107 t
ORIGIN      
        1 ccgttattta acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg
       61 aagccagaaa cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct
      121 tccctcaata cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt
      181 ttgtcatcca atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg
      241 tgtttgtccg actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg
      301 gcagataaag tgttgccagt ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg
      361 gtaagtacgc gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc
      421 gg
//
LOCUS       DAJVPT010000409          417 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVPT010000409 DAJVPT010000000
VERSION     DAJVPT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.415"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..83)
                     /locus_tag="OQM47_004320"
     CDS             complement(<1..83)
                     /locus_tag="OQM47_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1482198.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAV"
     gene            complement(58..246)
                     /locus_tag="OQM47_004321"
     CDS             complement(58..246)
                     /locus_tag="OQM47_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1482199.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(257..>417)
                     /locus_tag="OQM47_004322"
     CDS             complement(257..>417)
                     /locus_tag="OQM47_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCS1482200.1"
                     /translation="GFITPDDGSKDVFVHFTAIQSNEFRTLNENQKVEFSIEQGQRGP
                     AAANVVTL"
BASE COUNT          124 a           89 c          106 g           98 t
ORIGIN      
        1 acagcgttga agaaaaataa ggcagatata aaaaaggctc cacgagggag cctgatatta
       61 ttttgtgaaa tccagcggcg catggtcagt gcgtatttgc agcgcgacgt tatcaaaggt
      121 gatcatagtc gatttacaaa aaatacactt agcgcccaaa gggtttcgct ccgtcacatc
      181 gaatgccgat gtacggtact gtgaaccatg acaacatgga cagcggaaat ggagatgttg
      241 agtaataacg gcaaccttag agcgtaacaa cgttcgctgc cgcggggcca cgttgcccct
      301 gctcaataga aaattcaact ttctgatttt cgttcagcgt gcggaattca ttgctctgga
      361 tggcggtgaa atggacgaaa acgtctttgc tgccatcatc aggagtgata aagccaa
//
LOCUS       DAJVPT010000410          410 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVPT010000410 DAJVPT010000000
VERSION     DAJVPT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.416"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(1..410)
                     /locus_tag="OQM47_004323"
                     /pseudo
     CDS             complement(1..410)
                     /locus_tag="OQM47_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT          109 a           85 c          100 g          116 t
ORIGIN      
        1 taagcgctgg tactgctctg ggaaattagg atcgttaata tccaggatgt aacgtccatt
       61 agcagggaaa tgaactaatc cctggtgatc ttccagttcc aaccctaagg cactgtacca
      121 ggctccggta attaaccctc tcagggtgtc agagcgctca tagtttccca gatggtaata
      181 ggcgtctttg ttaaaaaaag gcagacgtga taatgacatt taaaggactg cgtatattct
      241 ttcgcccaga taatacaaac cgacgttgcg taggtgcgtt tgccttgttg ttttttacgg
      301 tgttgatctg cattaatttt cgcattcagc gagttacgga aacgagatat ctctccgggg
      361 atcaggttag ggaaacaggt aatgatatcc ccgttgtcaa tcacaggagg
//
LOCUS       DAJVPT010000411          401 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVPT010000411 DAJVPT010000000
VERSION     DAJVPT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.417"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>401
                     /locus_tag="OQM47_004324"
     CDS             <1..>401
                     /locus_tag="OQM47_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1482201.1"
                     /translation="TDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLG
                     VVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALK
                     LLSENGYDPVYGARPLKRAIQQQIENPLAQQ"
BASE COUNT          101 a           94 c          108 g           98 t
ORIGIN      
        1 gactgacggg caggggagaa cggtcgactt ccgtaatacg gttgtcatta tgacctctaa
       61 cctcggttcc gatctgattc aggaacgctt cggtgaactg gattatgcgc acatgaaaga
      121 gctggtgctc ggtgtggtta gccataactt ccgtccggaa ttcattaacc gtattgatga
      181 agtggtggtc ttccatccgc tgggtgaaca gcacattgcc tcgattgcgc agattcagtt
      241 gaaacgtctg tacaaacgtc tggaagaacg tggttatgaa atccacattt ctgacgaggc
      301 gctgaaactg ctgagcgaga acggttacga tccagtctat ggtgcacgtc ctttgaaacg
      361 tgctatccag cagcagatcg aaaacccgct ggcacagcaa a
//
LOCUS       DAJVPT010000412          397 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVPT010000412 DAJVPT010000000
VERSION     DAJVPT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.418"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          149 a           66 c           59 g          123 t
ORIGIN      
        1 gtttcttttt ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat
       61 caattatcaa tgcatagaaa acatattatg tttcgcgcta acgattatgc tgcgttacag
      121 acgaatggtt caattattct ttcccctaag tccgaatgtg gacatatata atttatggag
      181 aaaaacacgc atcaaattga taaaaggacg atatttcagt atgttgcaga taatgttatt
      241 tttgatgcat acgcgttata aatatcaaaa atacaaacca gataaatatc ttatcaatat
      301 acaacaaagt ttgaattatt caatttatag cagcacaacc aaacgccaaa aaataataac
      361 caagagctat aaataaggat taaaaatatt ttcagtg
//
LOCUS       DAJVPT010000413          395 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVPT010000413 DAJVPT010000000
VERSION     DAJVPT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.419"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>395
                     /locus_tag="OQM47_004325"
     CDS             <1..>395
                     /locus_tag="OQM47_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1482202.1"
                     /translation="VYGKVKAMHYMSDNASKDGDQSYIRFGFKGETQINDQLTGYGRW
                     EAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGD
                     SSAQTDNFMTKRASGLATYRNTDFFGVID"
BASE COUNT          104 a           79 c          109 g          103 t
ORIGIN      
        1 atgtctatgg caaagttaaa gccatgcatt atatgagtga taacgccagt aaagatggcg
       61 accagagtta tatccgtttt ggttttaaag gtgaaactca aattaacgat caactgactg
      121 gttatggtcg gtgggaagcg gagtttgccg gaaataaagc ggagagtgat actgcacagc
      181 aaaaaacgcg tctcgctttt gccgggttga aatataaaga tttgggttct ttcgattatg
      241 gtcgtaacct gggcgcgttg tatgacgtgg aagcctggac cgatatgttc ccggaatttg
      301 gtggcgattc ctcggcgcag accgacaact ttatgaccaa acgcgccagc ggtctggcga
      361 cgtatcggaa caccgacttc ttcggcgtta tcgat
//
LOCUS       DAJVPT010000414          393 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVPT010000414 DAJVPT010000000
VERSION     DAJVPT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.420"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            19..324
                     /locus_tag="OQM47_004326"
     CDS             19..324
                     /locus_tag="OQM47_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482203.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
BASE COUNT          123 a           84 c           95 g           91 t
ORIGIN      
        1 accggaggct ggagcataat gaccactttt accgacaaag aactgattaa agaaattaaa
       61 gagcgtatca gcagccttga cgtgcgagac gatattgagc gccgtgctta tgaaatcgca
      121 ctcctatctc tggaagtaga accagatgaa cgcgaagctt atgaattatt catggaaaag
      181 cgttttggtg acttagtaga tcgtcggaga gcaaaaaacg gcgataacga atacatggca
      241 tgggatatga ctctcggttg gatcgtctgg cagcaacgag ctggtatcca tttctcaaca
      301 atgtcacagc aagaggtgaa ataatggagc catacagcct cacactcgat gaggcctgtc
      361 attttctcaa gatatccaga ccgactgcca tta
//
LOCUS       DAJVPT010000415          393 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVPT010000415 DAJVPT010000000
VERSION     DAJVPT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.421"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..250)
                     /locus_tag="OQM47_004327"
     CDS             complement(<1..250)
                     /locus_tag="OQM47_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCS1482204.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGI"
BASE COUNT          125 a          105 c           83 g           80 t
ORIGIN      
        1 ggatcccccc ctcaccggcg ttatctgccc gcatcacgaa ggtgagatat ttaatggaaa
       61 ccacaaagat tagcagccag aagatcagcg ataaaaagcc aaacacggca tcgcgttcaa
      121 cgccaaaacc aaactggccg gacaaacatt cacgaagtgt atataacggg ctggtaccaa
      181 tatcgccgta gacaactcca atcgccgcga gggtaatcgc gggcaatgat tgcttattat
      241 cagtgctcat agactagtct ttcgttgaaa tatgaaatgt gtgcttagtc ccttggccca
      301 caaaaaagcg cacagtatgc acgattaacg gcaaaatcgt actcctaaat gcggccacat
      361 taacctggct caaagaaaaa gccccccctt tga
//
LOCUS       DAJVPT010000416          390 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVPT010000416 DAJVPT010000000
VERSION     DAJVPT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.422"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..360
                     /locus_tag="OQM47_004328"
     CDS             <1..360
                     /locus_tag="OQM47_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1482205.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLADSVMTPTY"
BASE COUNT          107 a           89 c           89 g          105 t
ORIGIN      
        1 aatggtaaag ttattgctcc agcttgtacc ctggtagctg cgacgaaaga ttcagtagtc
       61 acattgccaa atgtcagcgc caccaagttg caaactaatg gagcagtctc tggcgttaaa
      121 actgatgtac caattgcctt agaaggctgt gatgttactg taactaaaaa tgcgacgttt
      181 acctttagtg gtacggctga cggcgtgcaa cctacagcat ttgccaacca ggctaccacc
      241 gatgcagcga cgaatgttgc tctacaaatg tatctgccag atggttctac atcggtaact
      301 cccggtaccg aaacgtcgaa tattcagtta gcggattcgg taatgactcc aacttactga
      361 tagtgtttta tgttcagata atgcccgatg
//
LOCUS       DAJVPT010000417          389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVPT010000417 DAJVPT010000000
VERSION     DAJVPT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.423"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(122..>389)
                     /locus_tag="OQM47_004329"
     CDS             complement(122..>389)
                     /locus_tag="OQM47_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1482206.1"
                     /translation="KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYND
                     SAYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          112 a           90 c           77 g          110 t
ORIGIN      
        1 agtataatcg cggtaaataa cttacattaa actgttgttt taaagagcct gattaaacaa
       61 ctttttctgg acataacgac tccctggcct aagccaactg aataaaaagg cccttgataa
      121 attatttctt atattcgaca gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg
      181 tcgcattggt tgccacatag cgtgcaataa acttcatttc agccttgcct tcagcatcga
      241 tagcggtata agcagagtcg ttataaagtt taacctgcga attatcggac tggttaaaaa
      301 tacgaatacc aacgccggtt gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt
      361 tgcctgctgt aagcggcgta cccactttc
//
LOCUS       DAJVPT010000418          389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVPT010000418 DAJVPT010000000
VERSION     DAJVPT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.424"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>389
                     /locus_tag="OQM47_004330"
     CDS             <1..>389
                     /locus_tag="OQM47_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCS1482207.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIR"
BASE COUNT           80 a          103 c          120 g           86 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
      241 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      361 tctcccgcac aggcacagaa tattcgcga
//
LOCUS       DAJVPT010000419          388 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVPT010000419 DAJVPT010000000
VERSION     DAJVPT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.425"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>388
                     /locus_tag="OQM47_004331"
     CDS             <1..>388
                     /locus_tag="OQM47_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1482208.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEG"
BASE COUNT           98 a           98 c           95 g           97 t
ORIGIN      
        1 gcttgagcta ctctcacttc aacaacgacc tgtctgcaac catgagcaac ggtacttacg
       61 ttgacggtag caccaactcc gacgcttggg gcttcggttt gaaaaccggt tacgacttca
      121 aactgggtga tgctggttac gtgactcctt acggcagcat ttctggtctg ttccagtctg
      181 gtgatgacta ccagctgagc aacgacatga aagttgacgg tcagccttac gacagcatgc
      241 gttatgaact gggtgtagat gcaggttata ccttcaccta cagcgaagat caggctctga
      301 ctccgtactt caaactggct tacgtctacg acgactctaa caacgataac gatgtgaacg
      361 gtgattccat cgataacggt actgaagg
//
LOCUS       DAJVPT010000420          387 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVPT010000420 DAJVPT010000000
VERSION     DAJVPT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.426"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>387
                     /gene="curA"
                     /locus_tag="OQM47_004332"
     CDS             <1..>387
                     /gene="curA"
                     /locus_tag="OQM47_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1482209.1"
                     /translation="YLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDY
                     QPGDWVLGYSGWQDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPK
                     EGETLVVAAATEPVGATVGQIGKLKGC"
BASE COUNT           78 a           83 c          134 g           92 t
ORIGIN      
        1 tatttgtccc tggacccgta tatgcgtgga cgtatgagcg atgagccatc ttattcaccg
       61 ccggttgata ttggcggcgt gatggtcggc ggtacggtga gccgtgtcgt ggagtcgaat
      121 catcctgatt atcagcctgg cgactgggtg ctgggctaca gtggatggca ggactatgac
      181 atatccagtg gtgatgacct ggtgaaactt ggcgatcatc cgcaaaatcc atcgtggtcg
      241 ctgggtgtgc tggggatgcc aggctttacc gcttatatgg ggctgctgga tatcggtcag
      301 cctaaagagg gcgaaacgtt ggtggtagct gcggcgacag aaccagtggg ggcgacggtg
      361 gggcaaatcg gcaaacttaa aggttgc
//
LOCUS       DAJVPT010000421          384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVPT010000421 DAJVPT010000000
VERSION     DAJVPT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.427"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          127 a           65 c           72 g          120 t
ORIGIN      
        1 atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
       61 tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
      121 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
      181 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
      241 gattttcatt tatgtttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
      301 gataatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
      361 attcataagt tattgagttg atac
//
LOCUS       DAJVPT010000422          378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVPT010000422 DAJVPT010000000
VERSION     DAJVPT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.428"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>378)
                     /locus_tag="OQM47_004333"
     CDS             complement(<1..>378)
                     /locus_tag="OQM47_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1482210.1"
                     /translation="LSETAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIP
                     GAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRW
                     TDARPETTEPRYRAKSLTFYGING"
BASE COUNT           79 a          107 c           99 g           93 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc tgttgtgcct tttcgcctgc
      361 tgctgcggtt tcagacag
//
LOCUS       DAJVPT010000423          376 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVPT010000423 DAJVPT010000000
VERSION     DAJVPT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.429"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..276
                     /gene="metG"
                     /locus_tag="OQM47_004334"
     CDS             <1..276
                     /gene="metG"
                     /locus_tag="OQM47_004334"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCS1482211.1"
                     /translation="ENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQALIGRH
                     TIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
BASE COUNT           82 a           95 c           93 g          106 t
ORIGIN      
        1 gaaaacgcag agtttgttga aggttctgac aaactgctgc gcctgacgct ggatctcggc
       61 ggtgaaaaac gcaatgtctt ctccggtatt cgttctgctt acccggatcc gcaggcactg
      121 attggtcgtc acaccattat ggtggctaac ctggcaccac gtaaaatgcg cttcggtatc
      181 tctgaaggca tggtgatggc tgccggtcct ggcgggaaag atattttcct gctaagcccg
      241 gatgccggtg ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc gctgcatcga
      301 cagcgccttt tctttataaa ttcctaaagt tgttttcttg cgattttgtc tctctctaac
      361 ccgtataaat actggt
//
LOCUS       DAJVPT010000424          374 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVPT010000424 DAJVPT010000000
VERSION     DAJVPT010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.430"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(97..>374)
                     /locus_tag="OQM47_004335"
     CDS             complement(97..>374)
                     /locus_tag="OQM47_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCS1482212.1"
                     /translation="GAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQHLIFM
                     IWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT           94 a          107 c           84 g           89 t
ORIGIN      
        1 ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat cggttaaaca
       61 ggtagccgga ggatgttaca acctcctccg tcatctttaa cgtggtcgaa tcccctcaat
      121 aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc gcaacgtcgc
      181 gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat
      241 cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga cccaaccggc
      301 aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt catccattaa
      361 caacggcgcg cctg
//
LOCUS       DAJVPT010000425          373 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVPT010000425 DAJVPT010000000
VERSION     DAJVPT010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.431"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          132 a           69 c           53 g          119 t
ORIGIN      
        1 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
       61 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      121 gttagaaata cttaatgaaa aacagaaatc tcattcattt aatttataat taaatgatat
      181 ctcttcccgc agaatgcggg aatcacacaa tcaatattta ttcaataaaa tctgatgatt
      241 ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc gtaaaggcta
      301 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      361 ttcgcttttt atc
//
LOCUS       DAJVPT010000426          371 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVPT010000426 DAJVPT010000000
VERSION     DAJVPT010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.432"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(166..>371)
                     /locus_tag="OQM47_004336"
     CDS             complement(166..>371)
                     /locus_tag="OQM47_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1482213.1"
                     /translation="VYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGL
                     KRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT           88 a          118 c           74 g           91 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      301 cctctgatac atccctctcc aggtgatttg catcgccgtt tttctcactg tctccgttat
      361 gatgtacacc a
//
LOCUS       DAJVPT010000427          371 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVPT010000427 DAJVPT010000000
VERSION     DAJVPT010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.433"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>371)
                     /gene="queE"
                     /locus_tag="OQM47_004337"
     CDS             complement(<1..>371)
                     /gene="queE"
                     /locus_tag="OQM47_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1482214.1"
                     /translation="VVITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTW
                     VTVSPKLNMRGGYEVLSQALERANEIKHPVGRVRDIEALDELLATLTDDKPRVIALQP
                     ISQKDDATRLCIETCIARNWR"
BASE COUNT           81 a          103 c           88 g           99 t
ORIGIN      
        1 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
       61 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      121 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      181 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      241 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      301 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      361 aatcaccaca t
//
LOCUS       DAJVPT010000428          369 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVPT010000428 DAJVPT010000000
VERSION     DAJVPT010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.434"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..269
                     /locus_tag="OQM47_004338"
     CDS             <1..269
                     /locus_tag="OQM47_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482215.1"
                     /translation="TLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPR
                     YPLISELKQILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          110 a           85 c           96 g           78 t
ORIGIN      
        1 aaacgctgaa agctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag
       61 cagacttcct ggcgaacgtg gataaactgt ctgaagatgc attcgatgac cagtgcaccg
      121 gcgctaaccc gcgttacccg ctgatctccg agctgaaaca gattctgctg gatacctact
      181 acggtcgtga ttatgtagaa gatgaaactg cagcgaaaaa agaagccgct ccggctaaag
      241 ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct gtctggcaat ataaacggcc
      301 ccttctgggg taatgccgat cagttaagga tcagttgacc gatccagtgg ctgtgtaaga
      361 atccggaaa
//
LOCUS       DAJVPT010000429          367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVPT010000429 DAJVPT010000000
VERSION     DAJVPT010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.435"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(13..>367)
                     /locus_tag="OQM47_004339"
     CDS             complement(13..>367)
                     /locus_tag="OQM47_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1482216.1"
                     /translation="KTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSR
                     SNTIERVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWS
                     ALYVGSFSFVLPFFS"
BASE COUNT          120 a           80 c           80 g           87 t
ORIGIN      
        1 tttatgggca gtttagctaa aaaaaggcaa cacgaagcta aaactaccaa cataaagtgc
       61 tgaccagcta gcagctgaaa tcagactcac tgtatacact ttcactggat gaagttgcaa
      121 cattcctgcc accaaaggag caatgtaacg cagcacggcg ataaagcgcg aagtgaaaag
      181 tattgacaca gagttatttt gcaactgaag gcgaacccgt tctattgtat tggaacggct
      241 cgatattata catgcaagtt gagggatatg cccaattatt ataccaagat ggtaattaac
      301 gatagtaccg caccatgcgc cactcatgac cgtgattcca gcttcccatg gtgaaagagt
      361 cgtcttg
//
LOCUS       DAJVPT010000430          365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVPT010000430 DAJVPT010000000
VERSION     DAJVPT010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.436"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..294
                     /locus_tag="OQM47_004340"
     CDS             <1..294
                     /locus_tag="OQM47_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482217.1"
                     /translation="ALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIVHI
                     KGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT           85 a           74 c           94 g          112 t
ORIGIN      
        1 gcattgttaa ccggcgtgat ggtcgcctgt ctgccggaaa agatcgggtc acagaaaaat
       61 tatcgttgtc tggctggtgg cataggtggc gtcgttatca ccgagatcta ttgtgcagtt
      121 attgtacata ttaagggcat ggcttcctcg gagttgtttg aaaacattct ttctggtgac
      181 agtctcgttg tccgcatcat tcctgcattg ctggcaggtg tggtgatgag cagaatcatt
      241 acccgtctgc ccggattgga tatttcatgt cctgaaacag actctttaag ttaagcggga
      301 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
      361 ccgtt
//
LOCUS       DAJVPT010000431          364 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVPT010000431 DAJVPT010000000
VERSION     DAJVPT010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.437"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>364
                     /locus_tag="OQM47_004341"
     CDS             <1..>364
                     /locus_tag="OQM47_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1482218.1"
                     /translation="EPLYDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVR
                     DDRNAGSEMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHVRRKIHDVHARVPT"
BASE COUNT           81 a           99 c          110 g           74 t
ORIGIN      
        1 tggaaccgct gtatgacatc ctgcgccagt atgtgctgat gcccggtaaa gtccatgccg
       61 atgatatccc cgttccggtc caggagccgg gcagcggtaa aacaaggact gcccggctgt
      121 gggtctacgt tcgtgatgac cgtaacgccg gttcggaaat gcctccggcg gtctggttcg
      181 cgtactcacc ggaccggaaa ggtatccatc cacagaatca tctggccggt tacagcggag
      241 tgcttcaggc cgatgcttac ggtggttacc gggcgttgta cgaatccggc agaataacgg
      301 aagccgcatg tatggctcat gtccggagaa aaatccacga tgtgcatgca agagtgccaa
      361 ccga
//
LOCUS       DAJVPT010000432          360 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJVPT010000432 DAJVPT010000000
VERSION     DAJVPT010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.438"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(104..>360)
                     /locus_tag="OQM47_004342"
     CDS             complement(104..>360)
                     /locus_tag="OQM47_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCS1482219.1"
                     /translation="AEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNK
                     RFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          108 a           81 c           45 g          126 t
ORIGIN      
        1 caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa tataacaaaa
       61 atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc ccaaatcgtt
      121 tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct ttgcttttca
      181 ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg tttacatcaa
      241 ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct tcactaagta
      301 aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc atctctgcga
//
LOCUS       DAJVPT010000433          359 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVPT010000433 DAJVPT010000000
VERSION     DAJVPT010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.439"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          136 a           66 c           48 g          109 t
ORIGIN      
        1 ttgttgaaac aaatgacggt agagaatgtt aacactgccg ttaaacaata tctttctcat
       61 ccggtaaata cttataccgg agtattattg ccaaaataat aacagccccg tcaacacatc
      121 gttggcgggg attttagcaa tattcgctat ttttatgtaa taattttata aatgcgttca
      181 aaataataac caagtactaa tagtgatatt ttaatacctg atttttacgt gataattcag
      241 gaggcacaga aagcgcataa aaataacaac ataaaacacc ttgccaccac ccaagaattc
      301 tctattacat tgtttttaat gaaaaaatat cattccaaca atatctcacg ataaataac
//
LOCUS       DAJVPT010000434          352 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVPT010000434 DAJVPT010000000
VERSION     DAJVPT010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.440"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            84..>352
                     /gene="adrA"
                     /locus_tag="OQM47_004343"
                     /gene_synonym="yaiC"
     CDS             84..>352
                     /gene="adrA"
                     /locus_tag="OQM47_004343"
                     /gene_synonym="yaiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="catalyzes the conversion of 2 GTP into c-di-GMP;
                     adrA overexpression induces cellulose biosynthesis, cell
                     adherence to abiotic surfaces and swimming and swarming
                     motility; AdrA acts post-transcriptionally on the bcsABZC
                     operon activating cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1482220.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIAS"
BASE COUNT           71 a           93 c           98 g           90 t
ORIGIN      
        1 ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc cccgtctcta
       61 taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa aactttttca
      121 aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa catcagcggt
      181 ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg gctggcatgt
      241 tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg tggctggtgt
      301 tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc ag
//
LOCUS       DAJVPT010000435          350 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJVPT010000435 DAJVPT010000000
VERSION     DAJVPT010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.441"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>350
                     /locus_tag="OQM47_004344"
     CDS             <1..>350
                     /locus_tag="OQM47_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482221.1"
                     /translation="NVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVT
                     QHNTPDGGIPTIGLPGYSAPSAGAAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFE
                     HDINDNTTIRNTTR"
BASE COUNT           88 a          104 c           79 g           79 t
ORIGIN      
        1 tgaatgtgat gggcgaaaaa acccacgatg ccggacgcga caaagtcaaa aatgagcgtt
       61 acggcgtcgc cccttctgtc gcttttggcc ttggtacagc aaatcgtttg tatcttaatt
      121 atctgcatgt cactcagcac aacacgccag acggcggcat tccgaccatc ggtttgccgg
      181 gctattctgc cccatctgcg ggagcggcgg ccctgaatca ttccggaaaa gttgatactc
      241 ataactttta cggcacggat tccgattacg acgattcgac caccgacacc gccaccatgc
      301 gttttgagca cgacatcaac gataacacca ccattcgcaa tactacccgt
//
LOCUS       DAJVPT010000436          348 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVPT010000436 DAJVPT010000000
VERSION     DAJVPT010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.442"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            103..>348
                     /locus_tag="OQM47_004345"
     CDS             103..>348
                     /locus_tag="OQM47_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1482222.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTD"
BASE COUNT           90 a           80 c           81 g           97 t
ORIGIN      
        1 acgaacgtca aatatagttg tttactgtgc gctgttgtcc atgtgatatt gtgtaccaga
       61 tgtgtgccaa actattattt ctgagcgtac caaatattat ttatggctat aagcgacaca
      121 aaactgcgta ctatttatgg taaaccatat tcgggcccac aagaagtggc tgatgccgat
      181 ggcctcagcg tacgaatttc accgaagggg gtcatccagt tccagtaccg ctatcgctgg
      241 catggcaagc ctaatcgact tgggcttggt cgatacccat ccctgtcttt gaaggatgcc
      301 agacagatca ctgctgactt gcgaaagctc tatttctcag gaacggat
//
LOCUS       DAJVPT010000437          348 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVPT010000437 DAJVPT010000000
VERSION     DAJVPT010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.443"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT          102 a           97 c           65 g           84 t
ORIGIN      
        1 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
       61 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
      121 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
      181 catttaaatt tcacagacaa gaccgactct ttatggatac cggaaatgcg ccttcgtgtt
      241 gtgcccggtt ttatttcacc acttccgggc tttggtggta tctttactga agttctcaca
      301 caaccagtaa ggaaatgaat atgccaacgt atctcgccag agtagaac
//
LOCUS       DAJVPT010000438          347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVPT010000438 DAJVPT010000000
VERSION     DAJVPT010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.444"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>347)
                     /locus_tag="OQM47_004346"
     CDS             complement(<1..>347)
                     /locus_tag="OQM47_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1482223.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLL"
BASE COUNT           90 a          110 c           80 g           67 t
ORIGIN      
        1 gagcaggaag ccgagcacca cggcagtagc aagaccgagc attgcaccca gcggcggcca
       61 gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca gcccttccct
      121 tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa
      181 tgcgctatcg actgcctgtt ccagttgcac tttgacgttg cgcgacactt tgcgcatcca
      241 gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc
      301 ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg aagatcc
//
LOCUS       DAJVPT010000439          347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJVPT010000439 DAJVPT010000000
VERSION     DAJVPT010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.445"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>347
                     /locus_tag="OQM47_004347"
     CDS             <1..>347
                     /locus_tag="OQM47_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482224.1"
                     /translation="SIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLR
                     ARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELF
                     TTIEGNYADAVRLL"
BASE COUNT           80 a           95 c          106 g           66 t
ORIGIN      
        1 tcgattattg gcattcagtc gccgcgccct catggaccca tgcagacagc gacaaacctg
       61 gatgaagtct gcgaagcgca tctggcaacg ctacttgagc aacaaccgca cggcattgcg
      121 gcgcggctgc gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg
      181 ccagagacgc aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg
      241 gagattaacc gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag
      301 ttgtttacca ccattgaagg caactacgct gatgctgtgc gcctgct
//
LOCUS       DAJVPT010000440          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVPT010000440 DAJVPT010000000
VERSION     DAJVPT010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.446"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..114)
                     /locus_tag="OQM47_004348"
     CDS             complement(<1..114)
                     /locus_tag="OQM47_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HCS1482225.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPT"
BASE COUNT          112 a           69 c           74 g           87 t
ORIGIN      
        1 tgttggggga ctgatcctgc cattaatcgt taatgtggca gtctctgaag agactgcggg
       61 agaagccaat ccataggcaa gaaaaataat tcctgacagc caatacttat tcattgaacg
      121 ttatccctgt agtaaaggtt atgcctggat agaatgagtg cataacaaac tatagctgta
      181 catccactac acagcacgaa ggatgataat gaagcattgc ctgtacgatc actcgacttt
      241 gtagagtttc ggacgaaggt ccgcagaata ttcgcagtat taaataagtg ttcattaatg
      301 cggcaaaaac aaatacaccg catcaatata acatctctgc gg
//
LOCUS       DAJVPT010000441          337 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVPT010000441 DAJVPT010000000
VERSION     DAJVPT010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.447"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>337
                     /locus_tag="OQM47_004349"
     CDS             <1..>337
                     /locus_tag="OQM47_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCS1482226.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNA"
BASE COUNT          128 a           45 c           61 g          103 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaa
//
LOCUS       DAJVPT010000442          330 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVPT010000442 DAJVPT010000000
VERSION     DAJVPT010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.448"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>330)
                     /locus_tag="OQM47_004350"
     CDS             complement(<1..>330)
                     /locus_tag="OQM47_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482227.1"
                     /translation="LGDGSLSNGSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLIST
                     ARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGT
                     LVFSSAS"
BASE COUNT           88 a           93 c           64 g           85 t
ORIGIN      
        1 cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc gtttcaagca
       61 gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca cgagggatga
      121 atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc atgagtggat
      181 ttacctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag aagttgtaat
      241 caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa aacttcacgc
      301 tcccattgct aagactacca tccccgagaa
//
LOCUS       DAJVPT010000443          324 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVPT010000443 DAJVPT010000000
VERSION     DAJVPT010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.449"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>324)
                     /locus_tag="OQM47_004351"
     CDS             complement(<1..>324)
                     /locus_tag="OQM47_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482228.1"
                     /translation="LQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAETAP
                     VVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTAVG
                     FYRLAH"
BASE COUNT           86 a           89 c           83 g           66 t
ORIGIN      
        1 atgtgccagc cgataaaaac ccaccgcggt gaggtcattc gccagcaact ctgcctgatt
       61 aatagcgctc tgttcctgat agcgccagcc gttatggagc agttgaataa gtaacgcctg
      121 gcagcgcatc agcaactgat gagcagtaga cggcacaggc agaacgctgg cagaaggtag
      181 tgataccacc acaggcgcag tttctgcgtc cagcgcccag gcacgagtct ttgtcatcat
      241 tacctgtggt tccagtgtca attgcccatc aacaaaactg acaaagccag aaaccagaca
      301 cacggggtcg tctgtttgtt gcaa
//
LOCUS       DAJVPT010000444          324 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVPT010000444 DAJVPT010000000
VERSION     DAJVPT010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.450"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..99)
                     /locus_tag="OQM47_004352"
     CDS             complement(<1..99)
                     /locus_tag="OQM47_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane;protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCS1482229.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRP"
     gene            complement(138..>324)
                     /locus_tag="OQM47_004353"
     CDS             complement(138..>324)
                     /locus_tag="OQM47_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1482230.1"
                     /translation="GGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGV
                     NVFIQDVPGDQKEQIPD"
BASE COUNT           95 a           73 c           62 g           94 t
ORIGIN      
        1 cgggcggaat aaaaataagg cttccagcca aaaatcaatc cccagaacga aaactaacgc
       61 cgctagggat aatccctgaa gtagggttat ttcatgcatt tttattacct caaaccaata
      121 ttaaggcgat tttagattta gtcagggatt tgttcttttt gatcgccagg gacgtcctga
      181 atgaaaacat tcacgccacg ttgtttaata aaacgtaagt ccgcgagatc ctggtcatcg
      241 acataaactt tactgctgat ttgctttttc ccttccgaga aatgcatatt gccgacgttg
      301 acatctttca ggtcaatgcc acct
//
LOCUS       DAJVPT010000445          324 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVPT010000445 DAJVPT010000000
VERSION     DAJVPT010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.451"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..79)
                     /locus_tag="OQM47_004354"
     CDS             complement(<1..79)
                     /locus_tag="OQM47_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1482231.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVD"
BASE COUNT          134 a           45 c           40 g          105 t
ORIGIN      
        1 tatcaacgtc tgcagcaaat actccagcgc tcataaaaaa agaagaaaag acagcggcag
       61 caataattga acgtttcata atatgttcct taataaaatt tatttttggt tgttaagagt
      121 ctgtatctct tggaaacaaa tttctcacat caaaaaagct gccgcaatgt aaacatattg
      181 ttttattgat ttaaaacaaa ttaaccaatg caatataaat acaacaaata cttaagaata
      241 ttaaatattt atcacatcta ttaattaatg tagatttatg taaatggtat tgttgatatc
      301 aataaaaaag acgaaatcat ttat
//
LOCUS       DAJVPT010000446          323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJVPT010000446 DAJVPT010000000
VERSION     DAJVPT010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.452"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            5..>323
                     /locus_tag="OQM47_004355"
     CDS             5..>323
                     /locus_tag="OQM47_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005090636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1482232.1"
                     /translation="MEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWL
                     HKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMR
                     HEKM"
BASE COUNT          128 a           46 c           57 g           92 t
ORIGIN      
        1 agatatggag caacaagatg agagcgtgat taaaacctat acggggagaa tcacaattga
       61 gcatattctt cctcaagcat tagttaatga atactggatt aatagattcc aacctcaaga
      121 gcatgtctac tggctgcata aaataggtaa tttaacactc ataagtggtt ccaaaaattc
      181 tgaaacacaa cactatgatt ttatcaaaaa gaaatctatt tatgagaaac ttaatagtaa
      241 aagctctttt tatttaacaa aagatgtttg taattcttca gaatggggat tggctgaact
      301 gaaaatgaga catgaaaaaa tga
//
LOCUS       DAJVPT010000447          322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVPT010000447 DAJVPT010000000
VERSION     DAJVPT010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.453"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..>322
                     /locus_tag="OQM47_004356"
     CDS             <1..>322
                     /locus_tag="OQM47_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1482233.1"
                     /translation="FDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGM
                     VKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWA
                     APLVS"
BASE COUNT           62 a           76 c           86 g           98 t
ORIGIN      
        1 tttgatgcgg caacttatgc agctaactgg aatactgcgg ataccactga tggtcatgcg
       61 atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg gtgttgaatc cgcagcagta
      121 agtactggta tggttaaaaa cccgaaacgt accgttccgc tggcaaccat gctgggtact
      181 ggtttagcag gtattgttta tatcgctgcg actcaggtgc tttccggtat gtatccgtct
      241 tctgtaatgg cggcttccgg tgctccgttt gcaatcagtg cttcaactat cctcggtaac
      301 tgggctgcgc cgctggtttc tg
//
LOCUS       DAJVPT010000448          321 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVPT010000448 DAJVPT010000000
VERSION     DAJVPT010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.454"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            45..>321
                     /locus_tag="OQM47_004357"
     CDS             45..>321
                     /locus_tag="OQM47_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCS1482234.1"
                     /translation="MDWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINI
                     APLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAV"
BASE COUNT           80 a           65 c           92 g           84 t
ORIGIN      
        1 ggttaagtga taacagatgt ctggaaatat aggggcaaat ccagctggat tggggaatgc
       61 agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg ctggcctttg
      121 accatggtta ttttcagggg ccgactatcg gacttgaacg cattgatata aatatcgccc
      181 cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc
      241 ccccggcgac caataagccg gtggtactgc gggcgtcagg tgcgaactct attctggcgg
      301 aattaagtaa tgaagccgtg g
//
LOCUS       DAJVPT010000449          319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJVPT010000449 DAJVPT010000000
VERSION     DAJVPT010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.455"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..202
                     /locus_tag="OQM47_004358"
     CDS             <1..202
                     /locus_tag="OQM47_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1482235.1"
                     /translation="AQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARG
                     PMGLEALTTYKWIGIGDYTIRA"
BASE COUNT           72 a           82 c           85 g           80 t
ORIGIN      
        1 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
       61 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
      121 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
      181 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
      241 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
      301 aacatgtcct ttcagggcc
//
LOCUS       DAJVPT010000450          319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJVPT010000450 DAJVPT010000000
VERSION     DAJVPT010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.456"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>319)
                     /locus_tag="OQM47_004359"
     CDS             complement(<1..>319)
                     /locus_tag="OQM47_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HCS1482236.1"
                     /translation="FPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTPCTD
                     GYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLRDAR
                     FMRR"
BASE COUNT           79 a           96 c           80 g           64 t
ORIGIN      
        1 cggcgcataa aacgggcatc acgcaagcgc agcaccggca ttaaacgcac cagatggcgt
       61 gcctgatcgt taatatcctc aacatcaatc ggatgtccgt ctttatcgag aaaactgcgc
      121 agtgtcatca cgctgccgtc ttccgcactc tccccttcca gacgataaaa aatacggtga
      181 tagccatcgg tgcatggcgt ccagcacgct tccagcggcc gataacggcg aacccgatgt
      241 cggcctggca gcgattcgcg gaaggtcaaa ataatatgca gatgatgttc tcgcccgttg
      301 atatctcccg gcgggaacc
//
LOCUS       DAJVPT010000451          314 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJVPT010000451 DAJVPT010000000
VERSION     DAJVPT010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.457"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>314)
                     /locus_tag="OQM47_004360"
     CDS             complement(<1..>314)
                     /locus_tag="OQM47_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCS1482237.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     EL"
BASE COUNT           75 a           87 c           78 g           74 t
ORIGIN      
        1 cagttcagtc atcagcgtct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
       61 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
      121 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
      181 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
      241 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
      301 cagacgctcc agta
//
LOCUS       DAJVPT010000452          311 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJVPT010000452 DAJVPT010000000
VERSION     DAJVPT010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.459"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..196)
                     /locus_tag="OQM47_004361"
     CDS             complement(<1..196)
                     /locus_tag="OQM47_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482238.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT           93 a           74 c           70 g           74 t
ORIGIN      
        1 gcaatgaacc ggtatgaatt tgcagccata aatttacctg atcctttgga tgagcatgcg
       61 gataaatcat atatcgcaag tcattgtcca gttgcccggt aattaacttt tcatcctgcg
      121 gtaaggcggc ggcaatcagc cgcccgggga gcaacagagt tgccactaac gtcagtaaga
      181 aacagaggtt tctcataatt atctccatgc gaaaaccggg cgaatttacc cggttaagta
      241 aaatccgaac tattaaaatt tcatgctgac atcaagccag aaagtacggc cactggcgta
      301 ggtcgccaca c
//
LOCUS       DAJVPT010000453          310 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJVPT010000453 DAJVPT010000000
VERSION     DAJVPT010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.460"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..239)
                     /locus_tag="OQM47_004362"
     CDS             complement(<1..239)
                     /locus_tag="OQM47_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1482239.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKK"
BASE COUNT           88 a           60 c           84 g           78 t
ORIGIN      
        1 tccttctttc tgttggcata aaggatggcg tcaagttgag cgaatgaaac tgaatcgggc
       61 aatggccatg cgataccgga tgcaagaaat cgctgaaaaa gcgtatgtat tgtggaatga
      121 ctgagaccca gacgctgagc gatggcccgg atggtcagtt tatcttcaaa tcttaaacgc
      181 agggcatcag gcaaataaga acggaagcag ggaatatctt ttgttgtctg ggaattcatc
      241 gttcgtgtcc atcaatatag atgggcgcga ttgttgccag acaggacaat tttcacaaga
      301 cgtcgctgat
//
LOCUS       DAJVPT010000454          309 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJVPT010000454 DAJVPT010000000
VERSION     DAJVPT010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.461"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
BASE COUNT           82 a           54 c           75 g           98 t
ORIGIN      
        1 tcttcacttt ttaggccagt gcgtaagtgt tagtgtcagc tcctgccatt tcagcagagg
       61 aaattattct gctgatgaaa gctttggcat caggattcag cctcaaacgt atgcgtggtc
      121 gtcagaggca gttaggtcag aagcaataat tttagacagt gagaccatgt gcattggttt
      181 taaaaatgcc gtctatgttc atgattgcct tgatttgcat atggaacaac tggatttaga
      241 ttattgcggc tcaacaggcg tggttataga gaatgtaaac ggaggatttt ctttctcaaa
      301 ctcatggat
//
LOCUS       DAJVPT010000455          308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJVPT010000455 DAJVPT010000000
VERSION     DAJVPT010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.462"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..210
                     /locus_tag="OQM47_004363"
     CDS             <1..210
                     /locus_tag="OQM47_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482240.1"
                     /translation="AHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVPG
                     LVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT           75 a           71 c           79 g           83 t
ORIGIN      
        1 gcacactggc tggaacgtta tcagcaactc tggagccagc atcgtgaccg tgttatcggt
       61 acccaggaac tggtagcgat tgtgttggca gaaaaagacc actgggagca ggacctgacg
      121 caagtgcctg gtctggtcga gcaggttgct aatgacctgg atgcgattct tgaaaaaggg
      181 atgcgcgaag ctgtacgtcc gttgtgctaa taacaccgtt acttctgaac ccggtttcga
      241 ccgggtttct tatttcacta tgttagttac aacatcctat attcctttat cattaacagg
      301 tgtcacat
//
LOCUS       DAJVPT010000456          306 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJVPT010000456 DAJVPT010000000
VERSION     DAJVPT010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.463"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>306)
                     /locus_tag="OQM47_004364"
     CDS             complement(<1..>306)
                     /locus_tag="OQM47_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1482241.1"
                     /translation="YWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIA
                     AYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPGTPTGTYIVSPQFFITGCSEHSLP"
BASE COUNT           71 a           84 c           70 g           81 t
ORIGIN      
        1 ccggtaatga atgctctgaa catcccgtaa tgaaaaattg tggggatact atatacgttc
       61 cggtgggagt accgggcact gaaatcccca ccggtcccag tcgtgagcct ctgtatgact
      121 gccccctgac aagtctgatg actttatcac cgtcagcttc tccctggtac gcagcaataa
      181 tcagcccgcc aatgtcaggg tctccccatc tgttgcggac agagctcgcc acgattctgt
      241 aaataatatc ttctgtggtt atatttattt tcacccagcc agtcagtctg gatatgggcc
      301 agtagc
//
LOCUS       DAJVPT010000457          303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJVPT010000457 DAJVPT010000000
VERSION     DAJVPT010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.denovo.464"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..>303)
                     /locus_tag="OQM47_004365"
     CDS             complement(<1..>303)
                     /locus_tag="OQM47_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HCS1482242.1"
                     /translation="VGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLR
                     NAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTAS"
BASE COUNT           83 a           85 c           82 g           53 t
ORIGIN      
        1 gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat attgttcata
       61 atagaagaga ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca caggccgtta
      121 tccgccagca cgttgagtac gccagaaaga tattccgtta atccggcatt gcgcaggcca
      181 taaaccacca gatacatgcc gagcgagaag atgacaatct gccagggggc accgcgcagg
      241 actttacccg tattaatcgc atgaccgcgt ttagcgacga caaataatat cagtgcgccc
      301 aca
//
LOCUS       DAJVPT010000458         5999 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.173, whole genome shotgun sequence.
ACCESSION   DAJVPT010000458 DAJVPT010000000
VERSION     DAJVPT010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.173"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..727
                     /locus_tag="OQM47_004366"
     CDS             <1..727
                     /locus_tag="OQM47_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1482243.1"
                     /translation="AEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINA
                     ELFALAGLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHL
                     IALRIMQQVKATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDY
                     SRLVQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCQGRPD"
     gene            789..1403
                     /locus_tag="OQM47_004367"
                     /pseudo
     CDS             789..1403
                     /locus_tag="OQM47_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1460..2917)
                     /gene="pepD"
                     /locus_tag="OQM47_004368"
     CDS             complement(1460..2917)
                     /gene="pepD"
                     /locus_tag="OQM47_004368"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1482244.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3179..3637
                     /gene="gpt"
                     /locus_tag="OQM47_004369"
     CDS             3179..3637
                     /gene="gpt"
                     /locus_tag="OQM47_004369"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1482245.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3729..4973
                     /gene="frsA"
                     /locus_tag="OQM47_004370"
     CDS             3729..4973
                     /gene="frsA"
                     /locus_tag="OQM47_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1482246.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5031..5432
                     /gene="crl"
                     /locus_tag="OQM47_004371"
     CDS             5031..5432
                     /gene="crl"
                     /locus_tag="OQM47_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1482247.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5471..>5999)
                     /locus_tag="OQM47_004372"
     CDS             complement(5471..>5999)
                     /locus_tag="OQM47_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1482248.1"
                     /translation="ENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDTVAVGMTYQF"
BASE COUNT         1437 a         1478 c         1596 g         1488 t
ORIGIN      
        1 tgctgaagaa agctggctgg aggaaaacct gccgtcagca tttgcacagc atccgtggcg
       61 cagctcgctt ggctctatcg gtggtggcaa tcatttcgct gaactgcaac aagttgatca
      121 aatcatcaac gctgaactgt ttgcactggc aggtctggat gcgcagcatc tgcaactgct
      181 ggttcatagc ggctcgaggg ggctagggca gtctatttta cagcggcata ttgcctcgtt
      241 ttcgcatcat ggtttgcctg aaggcagtga cgacgcgctg gcgtttgcgc gttttaatcg
      301 ccacctgatc gctttgcgca taatgcaaca ggttaaggcc accggaagtc cggttctgga
      361 tgtggcgcat aactttgtta gcgcgtgtcg aatcggtgat caacaagggg tcttacatcg
      421 taaaggtgcc acgcctgatg actgtggtct ggtggttatt cccggttcac gcggtgatta
      481 ctccaggctg gttcagcccg tcaggagtga ggaaacattg cattcgctgg cgcatggggc
      541 tgggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac
      601 gcaactctcg cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
      661 cgaagaagcg ccacaagcct ataaatcggc tgaaagtgtg gtgcaatgtc agggcaggcc
      721 ggattaatta ttcctgtcgc gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg
      781 aaaaaaggat gatcttgcta taactctcct cagcgcaggg gccggaagaa tgttgtctcg
      841 cagtgaaaaa ggcgctggac aggctaatta aagaagctgc ccgacaagac gtcgcggtaa
      901 cggtgctgga aacagaaaca ggccgctact ctgacacgct gcgttcggcg ctgatttctc
      961 tggatggcga caacgcatgg gcgttaagcg aaagctggtg tggcactatt cagtggattt
     1021 gcccgagccc gtatcgccca catcacgggc gtaaaaactg gtttctgggc attgggcgtt
     1081 ttaccgctga tgagcaggag caatccgatg cgatccggta tgagacgctg cgttcgtcgg
     1141 ggccgggcgg tcaacatgtc aataaaaccg actcggcggt acgcgccacg catttggcat
     1201 ccggtattag cgtgaaggtt cagtcagagc gcagccagca tgctaacaaa cggctggcgc
     1261 gattgctgat tgcctggaag ctggatcagc agcaatagga aaatagcgcg gtgctgaaat
     1321 cacagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacga acgtttacag
     1381 ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggcgat taaacgccat
     1441 ccggcagcaa atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg
     1501 tccagtaatg acctacgctt tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga
     1561 tagttggccc gatagaaacc atgtccattt ccggatacgg ttttttgaac agaccacatt
     1621 ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt
     1681 cacgtaccag atgcatcacc ggagaattag cgtccggctg ccagccagga tatgcgcctt
     1741 tcgcttcggt tttcgcgcca gccagtttac ccagcgaatc cagcatgctc accacgtagt
     1801 ctttaccgct gtcgattagt gaacggatca ggcagtgaat ttctacgtta ttgtcagtca
     1861 tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg tcggaattgc
     1921 ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa
     1981 tcagggcagc tttatcgttc gctacagagt ccagcaacaa ggccagattt ttctctttct
     2041 ctgccagctc gtttttcagg atctcctgat aggtattcac cagagatttc agtgcgtcga
     2101 ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg
     2161 tgccgccgtt gaaatcgata aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc
     2221 gcaccagcag tttgttggca ttacccaggc caacgtggat ttcaccgccg gagtgaccgc
     2281 ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc agctggaacc gcttcacgat
     2341 ctaaatgcag gttggaggtg aagtcgatac cccccgcaca acccatgtag atttcacctt
     2401 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccga
     2461 acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa
     2521 ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac
     2581 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
     2641 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgta
     2701 agacgaccgg tttacgattt tccatacctg cggtagcagg ttgacgaatc aggatattac
     2761 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
     2821 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
     2881 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
     2941 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
     3001 tgcgagatat gtattgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     3061 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     3121 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3181 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3241 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3301 accgggggcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3361 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     3421 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
     3481 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3541 ggttgatgac tatgttgttg atatcccgca agatacctgg atcgaacagc cgtgggatat
     3601 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcattgcc
     3661 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
     3721 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3781 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3841 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3901 ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     3961 ctgaacgtac cgacgatgat ttatacgata ccgtgattgg ctaccgtggt ggcaactgga
     4021 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtacg gaagaagatc
     4081 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
     4141 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
     4201 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4261 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4321 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4381 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa
     4441 aatggaagct cacccaggac tccagcctgt tgcatcagca cgtcttaaag gcgctgccta
     4501 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4561 ttgccgtgcg tctggcatac cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4621 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4681 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4741 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     4801 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4861 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4921 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4981 ctaaattttg ccaatttggt aaaacagttg catcacaaca ggagatagca atgacgttac
     5041 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     5101 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     5161 tgaaaccggc accggaagtg cgtgaattct ggggctggtg gatggagctg gaagcgcagg
     5221 aatcccgttt tacctacagc taccagtttg gtctgttcga taaagcaggc gactggaaga
     5281 gtgttccggt aaaagatact gaagtggttg aacgactgga gcacaccctg cgtgagtttc
     5341 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcgaatgatt
     5401 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgc atcacatccg
     5461 gcaatattca ttaaaactga tacgtcatcc caaccgcgac agtatcatca ttattaatat
     5521 tcagcttatt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     5581 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatgc
     5641 cctcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5701 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5761 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5821 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5881 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5941 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcg
//
LOCUS       DAJVPT010000459         5818 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.178, whole genome shotgun sequence.
ACCESSION   DAJVPT010000459 DAJVPT010000000
VERSION     DAJVPT010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.178"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OQM47_004373"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OQM47_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1482249.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OQM47_004374"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OQM47_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OQM47_004375"
     CDS             2144..2617
                     /locus_tag="OQM47_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1482250.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OQM47_004376"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OQM47_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OQM47_004377"
     CDS             2967..3803
                     /locus_tag="OQM47_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1482251.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OQM47_004378"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OQM47_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OQM47_004379"
     CDS             complement(4358..4747)
                     /locus_tag="OQM47_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1482252.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OQM47_004380"
     CDS             4965..5405
                     /locus_tag="OQM47_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1482253.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1399 a         1539 c         1517 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggataaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcattcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DAJVPT010000460         5716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.179, whole genome shotgun sequence.
ACCESSION   DAJVPT010000460 DAJVPT010000000
VERSION     DAJVPT010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.179"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            complement(<1..1397)
                     /locus_tag="OQM47_004381"
     CDS             complement(<1..1397)
                     /locus_tag="OQM47_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1482254.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSEL"
     gene            complement(1400..1960)
                     /locus_tag="OQM47_004382"
     CDS             complement(1400..1960)
                     /locus_tag="OQM47_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1482255.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2086..2436)
                     /locus_tag="OQM47_004383"
     CDS             complement(2086..2436)
                     /locus_tag="OQM47_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1482256.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTSEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2639..3652)
                     /gene="intI1"
                     /locus_tag="OQM47_004384"
     CDS             complement(2639..3652)
                     /gene="intI1"
                     /locus_tag="OQM47_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1482257.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3862..4692
                     /locus_tag="OQM47_004385"
     CDS             3862..4692
                     /locus_tag="OQM47_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1482258.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            4805..5596
                     /gene="aadA1"
                     /locus_tag="OQM47_004386"
     CDS             4805..5596
                     /gene="aadA1"
                     /locus_tag="OQM47_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1482259.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1331 a         1556 c         1601 g         1228 t
ORIGIN      
        1 ttcaactcgg acagcgcgca cagttcgtag tagcgccggt cgatgccggc gtcggtcatc
       61 accagtttct gccagcgcgg cttgatgaag ccggtcggtg catcggctgg cagcttgcgg
      121 gcgttgtcgg tgttcatgcc gcgcagcacc tcaatggcat caagcacgtt tttggcggcg
      181 ggcgcggccc gcagcttgag cacggcaagg aattccggtg catagcggcg cagggtggcg
      241 tagctctcgc cgatgcgatg caggaaatcg aagtcatcgg gttgcgcgag cttctgcgcc
      301 tcggtgacgc tctcggcaaa ggaatcccag gacatgacgg cctcgatggc ggcaaacgca
      361 tcgcggcctg attgcttggc gtcgatcagc gcctgaccga tgcgcccgta cagacgtacc
      421 ttggcgttga tggccttgcc tgacgcctgg aactgctgct gatgcttatt cttggcagcg
      481 ttaaacagct tacccaggat gcggtcgtgc aggtcgatga tttcgtcggt gacggtggcc
      541 atgccctcgg tggccagcgc cacgagagtg gcgtagcgcc gttgcggctc gaatttggcc
      601 aggtcggcgg gtgtcatctg gccgccctcg cgggcaatct tgagcaggcg gttctggtga
      661 accagccgct cgatgccggt aggcagatcg agtgcctgcc atgccttgag gcgttcgatg
      721 tgttccagca tatgccgcga atttggcttg gccggagact ggcgcaacca agccaaccag
      781 gtcgtcttgc cgttgtcccg gcgcttgagc agatcgtcga ggcggcggcg atgcgcgtcc
      841 gccagtggtt cggccaaggc gtcgtagatg cgccggttag cacgggtgat cgcctcggca
      901 ctcgcccgct cgacggcgtt gagggcgggc agaatgaccg actgccgccg caggtgcccg
      961 atcaaggcgc tggccagcac gatgcctttg tcggtttgca tcgccagctc ggtcagcatc
     1021 tggacggcct gccggtaatg gctcatggtg aagggccgga aaccgaacac ggtttgcagc
     1081 tcgctcaggt gctcgcgccg ggtctgctcc cgctggccgt actcgttcca gctttcgacg
     1141 ccgaccttga gctggtcggc gaccagcttc aacaagggcg ggaacggtag ttcatcgacg
     1201 cccaggatga cgccgggaaa gcgcaggtaa cagagctgca ccgcgaagcc cagccgattg
     1261 gctggcccgc gccgctgtcg gatgatcgag aggtcggtat cgttgaatgt gtaatgtcgg
     1321 atcaggtcgt ccttggagtc cggcaacgcc agcaggcttt cccgctcggc ggcggacagg
     1381 atggaacgac gtggcatatt tactgatccg ttctcaagta ttgatacagg gtttcgcgac
     1441 tgattccgaa ttcacgagca agcttggtct tttgctcgcc agcctcgaca cgttggcgca
     1501 gttcggcaat acgctcagac gacagggatt tcttcctgcc acggtaagcc ccgcgttgct
     1561 tggcgagcgc aataccctcg cgctgacgct cgcggatcag ggcgcgctcg aactcggcga
     1621 acgcgcccat caccgagagc atcaggttcg ccatcggaga gtcttcgcca gtaaaactga
     1681 ggtgttcctt gacgaattcg atatgcacgc cgcgttgtgt cagcgtttgc acgatccggc
     1741 gcaaatcatc gagattgcgc gccaggcgat ccatgctatg caccaccacg gtgtcgccgg
     1801 tgcgggcgaa gcttatcagc gcttccagtt gcggacgctt gacatccttg ccggatgcct
     1861 tgtcgctaaa agcgcgatca accttgacgc cttccagttg ccgttccggg ttctggtcga
     1921 aggtgctgac cctgatatac ccaatgcgct gtccagtcat ggaattccct gcaaaatgtc
     1981 agggaagact ctatgacctt caacgagata tgtcaataaa ttcaaaattc aatcctatcc
     2041 tgacgcaatt tacacatggc atctgacatc aggttagggt atgcctcaac ctgacggcgg
     2101 cgaatcacaa gcgtccggtt tgacgctggt ttcggtcgca gtgctggccc gcgcctggaa
     2161 gcgctgatag cactccagcc cgcagaaatg ctccacgtac tcggcccctt ccgacgtgaa
     2221 ggcggcatcg agcgggattt ccttgcagca cacgcagcaa ctggtgctcg gttcattggc
     2281 gttcatggtg gtgttcctcc attggttgac gaagccgacg aaggccgccg ccggcatcgg
     2341 cctggcgaac aggaaaccct gcaccgtgtc gcaacccgcc tgccgcaacc acgcaaggca
     2401 gtcgggtgtt tccacaccct cggctaccac ctccattgtc gttttcagaa gacggctgca
     2461 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatccat caggcaacga
     2521 cgggctgctg ccggccatca gcggacgcag ggaggacttt ccgcaaccgg ccgttcgatg
     2581 cggcaccgat ggccttcgcg caggggtagt gaatccgcca ggattgactt gcgctgccct
     2641 acctctcact agtgaggggc ggcagcgcat caagcggtga gcgcactccg gcaccgccaa
     2701 ctttcagcac atgcgtgtaa atcatcgtcg tagagacgtc ggaatggccg agcagatcct
     2761 gcacggttcg aatgtcgtaa ccgctgcgga gcaaggccgt cgcgaacgag tggcggaggg
     2821 tgtgcggtgt ggcgggcttc gtgatgcctg cttgttctac ggcacgtttg aaggcgcgct
     2881 gaaaggtctg gtcatacatg tgatggcgac gcacgacacc gctccgtgga tcggtcgaat
     2941 gcgtgtgctg cgcaaaaacc cagaaccacg gccaggaatg cccggcgcgc ggatacttcc
     3001 gctcaagggc gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc ttcagccacc
     3061 atgcccgtgc acgcgacagc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca
     3121 aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca
     3181 gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca tacagaagct
     3241 gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg
     3301 tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga agccagggca
     3361 gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg
     3421 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
     3481 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     3541 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     3601 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     3661 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3721 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3781 ctaaaacaaa gttgggcgaa cccggagcct cattaattgt tagccgttaa aattaagccc
     3841 tttaccaaac caatacttat tatgaaaaac acaatacata tcaacttcgc tattttttta
     3901 ataattgcaa atattatcta cagcagcgcc agtgcatcaa cagatatctc tactgttgca
     3961 tctccattat ttgaaggaac tgaaggttgt tttttacttt acgatgcatc cacaaacgct
     4021 gaaattgctc aattcaataa agcaaagtgt gcaacgcaaa tggcaccaga ttcaactttc
     4081 aagatcgcat tatcacttat ggcatttgat gcggaaataa tagatcagaa aaccatattc
     4141 aaatgggata aaacccccaa aggaatggag atctggaaca gcaatcatac accaaagacg
     4201 tggatgcaat tttctgttgt ttgggtttcg caagaaataa cccaaaaaat tggattaaat
     4261 aaaatcaaga attatctcaa agattttgat tatggaaatc aagacttctc tggagataaa
     4321 gaaagaaaca acggattaac agaagcatgg ctcgaaagta gcttaaaaat ttcaccagaa
     4381 gaacaaattc aattcctgcg taaaattatt aatcacaatc tcccagttaa aaactcagcc
     4441 atagaaaaca ccatagagaa catgtatcta caagatctgg ataatagtac aaaactgtat
     4501 gggaaaactg gtgcaggatt cacagcaaat agaaccttac aaaacggatg gtttgaaggg
     4561 tttattataa gcaaatcagg acataaatat gtttttgtgt ccgcacttac aggaaacttg
     4621 gggtcgaatt taacatcaag cataaaagcc aagaaaaatg cgatcaccat tctaaacaca
     4681 ctaaatttat aaaaaatcta atggcaaaat cgcccaaccc ttcaatcaag tcgggacggc
     4741 caaaagcaag cttttggctc ccctcgctgg cgctcggcgc cccttatttc aaacgttaaa
     4801 catcatgagg gaagtggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     4861 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     4921 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     4981 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     5041 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     5101 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     5161 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     5221 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     5281 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     5341 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     5401 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     5461 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     5521 ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa
     5581 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
     5641 taactcaagc gttagatgca ctaagcacat aattgcggtg atgctgccaa cttactgatt
     5701 tagtgtatga tggtgt
//
LOCUS       DAJVPT010000461         5322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.186, whole genome shotgun sequence.
ACCESSION   DAJVPT010000461 DAJVPT010000000
VERSION     DAJVPT010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.186"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            <1..792
                     /locus_tag="OQM47_004387"
     CDS             <1..792
                     /locus_tag="OQM47_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS1482260.1"
                     /translation="VGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSS
                     VGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLV
                     NQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVF
                     NTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRD
                     ADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            785..1519
                     /gene="frdB"
                     /locus_tag="OQM47_004388"
     CDS             785..1519
                     /gene="frdB"
                     /locus_tag="OQM47_004388"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1482261.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1530..1925
                     /gene="frdC"
                     /locus_tag="OQM47_004389"
     CDS             1530..1925
                     /gene="frdC"
                     /locus_tag="OQM47_004389"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1482262.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1936..2295
                     /gene="frdD"
                     /locus_tag="OQM47_004390"
     CDS             1936..2295
                     /gene="frdD"
                     /locus_tag="OQM47_004390"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1482263.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2358..3491
                     /locus_tag="OQM47_004391"
     CDS             2358..3491
                     /locus_tag="OQM47_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1482264.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3580..4113
                     /gene="blc"
                     /locus_tag="OQM47_004392"
     CDS             3580..4113
                     /gene="blc"
                     /locus_tag="OQM47_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1482265.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4110..4427)
                     /gene="sugE"
                     /locus_tag="OQM47_004393"
     CDS             complement(4110..4427)
                     /gene="sugE"
                     /locus_tag="OQM47_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1482266.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4603..4749)
                     /gene="ecnB"
                     /locus_tag="OQM47_004394"
     CDS             complement(4603..4749)
                     /gene="ecnB"
                     /locus_tag="OQM47_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1482267.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4860..4985)
                     /gene="ecnA"
                     /locus_tag="OQM47_004395"
     CDS             complement(4860..4985)
                     /gene="ecnA"
                     /locus_tag="OQM47_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1482268.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5037..>5322)
                     /locus_tag="OQM47_004396"
     CDS             complement(5037..>5322)
                     /locus_tag="OQM47_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1482269.1"
                     /translation="ADAKAIGDNAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDT
                     DPGLKGDTAGTGGKPATLSTGAVVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT         1330 a         1386 c         1390 g         1216 t
ORIGIN      
        1 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgactgcaca ctacaccatg
       61 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
      121 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
      181 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
      241 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
      301 ctagttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
      361 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
      421 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
      481 aacaccgacc tgctctacac cattgaactg agccacggtc tgaacgttgc tgaatgtatg
      541 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
      601 tgcaccgagc gtgacgacgt caactttctc aaacacaccc tcgccttccg cgatgctgat
      661 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
      721 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa taaggagaag
      781 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
      841 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
      901 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
      961 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     1021 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     1081 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     1141 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     1201 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     1261 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     1321 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     1381 gaacggtgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     1441 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     1501 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     1561 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     1621 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgcgctg tttgccctga
     1681 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     1741 tcattaacct gatcactctg acggcagctc tgctgcacac caaaacctgg tttgaactgg
     1801 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     1861 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     1921 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     1981 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     2041 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     2101 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     2161 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtgcct
     2221 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     2281 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     2341 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     2401 actccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     2461 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     2521 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     2581 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     2641 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta
     2701 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     2761 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     2821 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     2881 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     2941 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     3001 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     3061 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttac
     3121 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     3181 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     3241 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     3301 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     3361 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     3421 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     3481 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     3541 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     3601 gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc
     3661 gtgaccgtag tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc
     3721 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     3781 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag
     3841 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     3901 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     3961 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     4021 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     4081 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     4141 cccaatacga ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc
     4201 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     4261 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     4321 acggtaataa cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc
     4381 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     4441 aatttggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     4501 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca
     4561 gaaaaaacaa acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt
     4621 tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     4681 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     4741 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     4801 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     4861 tagctggcag cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa
     4921 gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc
     4981 atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     5041 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt
     5101 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     5161 cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     5221 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     5281 agcagccatt tagcattgtc gccaattgct tttgcatcag ca
//
LOCUS       DAJVPT010000462         4806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.195, whole genome shotgun sequence.
ACCESSION   DAJVPT010000462 DAJVPT010000000
VERSION     DAJVPT010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.195"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            95..499
                     /locus_tag="OQM47_004397"
     CDS             95..499
                     /locus_tag="OQM47_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCS1482270.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(477..1100)
                     /gene="tetR(B)"
                     /locus_tag="OQM47_004398"
     CDS             complement(477..1100)
                     /gene="tetR(B)"
                     /locus_tag="OQM47_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1482271.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1182..2387
                     /gene="tet(B)"
                     /locus_tag="OQM47_004399"
     CDS             1182..2387
                     /gene="tet(B)"
                     /locus_tag="OQM47_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1482272.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2500..3093)
                     /gene="tetC"
                     /locus_tag="OQM47_004400"
     CDS             complement(2500..3093)
                     /gene="tetC"
                     /locus_tag="OQM47_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1482273.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3226..3597
                     /locus_tag="OQM47_004401"
     CDS             3226..3597
                     /locus_tag="OQM47_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1482274.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3607..>4806)
                     /locus_tag="OQM47_004402"
     CDS             complement(3607..>4806)
                     /locus_tag="OQM47_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1482275.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1285 a          908 c         1034 g         1579 t
ORIGIN      
        1 attagattgc cagctaatca cagtagtagc tcaaggcaag catcgttatt ttcggctagc
       61 aggaaaagat attgctgaat tgatggaaag tatgatgggg atctccttaa accatggcgt
      121 acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct atgatcattt
      181 agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat ggatcactga
      241 aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg gaattgacgt
      301 tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg aacgccgttt
      361 ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta aagggtggtt
      421 aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg ctgcttttaa
      481 gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat
      541 aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc
      601 ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct
      661 tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct
      721 agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt
      781 tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat
      841 ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa
      901 aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta
      961 ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg
     1021 gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta
     1081 cttttatcta atctagacat cattaattcc taatttttgt tgacactcta tcattgatag
     1141 agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt tcgacaaaga
     1201 tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg ccagtcttgc
     1261 caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt ggcgtattgc
     1321 ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa atgtctgacc
     1381 gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg gattacttat
     1441 tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt tcagggatca
     1501 caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca gcttctcaac
     1561 gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata gcggggccta
     1621 ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc gctgcgttgc
     1681 taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa aatacacgtg
     1741 ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac atcactttat
     1801 ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata ggccaaattc
     1861 ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc atgatggttg
     1921 gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt gtggcaggaa
     1981 gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt gcagatagta
     2041 gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct gttttaattt
     2101 tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct atccaaacaa
     2161 agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat gcaaccggtg
     2221 ttattggccc attactgttt gctgttattt ataatcattc actaccaatt tgggatggct
     2281 ggatttggat tattggttta gcgttttact gtattattat cctgctatcg atgaccttca
     2341 tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta tttcgtcacc
     2401 aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg gcatttttta
     2461 cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt tcaagctcaa
     2521 taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct cttttaggtt
     2581 tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa gcacatcatc
     2641 atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt ttaccttacc
     2701 agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag gcatcacttc
     2761 ttggataggg ataaggtttt tattattatc aataatataa tcaagataat gttcaaatat
     2821 actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt aggcatcaat
     2881 cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt taaagtgatg
     2941 ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg aaacagcttg
     3001 atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat aggtactctt
     3061 aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac ataccgtcag
     3121 tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt aggttatttt
     3181 atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca attaagatat
     3241 gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat tgaacataat
     3301 ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac caagtggtat
     3361 tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat tcgtgctcgt
     3421 cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct tgagatcgca
     3481 ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta catttttaag
     3541 gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat gcactgagag
     3601 atcccctcat aatttcccca aagcgtaacc atgtgtgaat aaattttgag ctagtagggt
     3661 tgcagccacg agtaagtctt cccttgttat tgtgtagcca gaatgccgca aaacttccat
     3721 gcctaagcga actgttgaga gtacgtttcg atttctgact gtgttagcct ggaagtgctt
     3781 gtcccaacct tgtttctgag catgaacgcc cgcaagccaa catgttagtt gaagcatcag
     3841 ggcgattagc agcatgatat caaaacgctc tgagctgctc gttcggctat ggcgtaggcc
     3901 tagtccgtag gcaggacttt tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga
     3961 atagatatta acaagttgtt tgggtgttcg aatttcaaca ggtaagttag ttgctagaac
     4021 ccatggctcc tttgccgacg ctgagtagat tttaggtgac gggtggtgac aatgagtccg
     4081 tgtcgagcgc tgattttttc ggcctttaga gcgagattta tacaatagaa tttggcatga
     4141 gattggattg cttttagtca gcctcttata gcctaaagtc tttgagtgac tagatgacat
     4201 atcatgtaag ttgctgatag gtttccagtt ttccgctcct aggtctgcat attgtacttt
     4261 tcctcttact cgacttaacc agtaccaacc cagcttctca acggatttat accatggcac
     4321 tttaaagcca gcatcactga caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag
     4381 gtcggctaga aattggtcat gagctttctt tgaacattgc tctgaaagcg ggaacgcttt
     4441 ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg
     4501 tttttgctca cgaatatcag accagtcaac aagtacaatg ggcatcgtat tgcccgaaca
     4561 gataaagcta gcatgccaac ggtatacagc gagtcgctct ttgtggaggt gacgattacc
     4621 taacaatcgg tcgattcgtt tgatgttatg ttttgttctc gctttggttg gcaggttacg
     4681 gccaagttcg gtaagagtga gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa
     4741 gctgttgagt cgttttaagt gtaattcggg gtaatgactc caacttactg atagtgtttt
     4801 atgttc
//
LOCUS       DAJVPT010000463         3176 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103218
            SAMEA8899863-rid15854153.guided.223, whole genome shotgun sequence.
ACCESSION   DAJVPT010000463 DAJVPT010000000
VERSION     DAJVPT010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899863
            Sequence Read Archive: ERR6005108
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478022.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:34:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,405
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 171 of 370
            Pseudo Genes (incomplete)         :: 99 of 370
            Pseudo Genes (internal stop)      :: 142 of 370
            Pseudo Genes (multiple problems)  :: 41 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899863-rid15854153.guided.223"
                     /serovar="S. flexneri 2a"
                     /isolate="103218"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-13"
     gene            263..922
                     /locus_tag="OQM47_004403"
     CDS             263..922
                     /locus_tag="OQM47_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1482276.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OQM47_004404"
     CDS             complement(1123..1500)
                     /locus_tag="OQM47_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1482277.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>3176)
                     /locus_tag="OQM47_004405"
     CDS             complement(1567..>3176)
                     /locus_tag="OQM47_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1482278.1"
                     /translation="RRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTS
                     LKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLD
                     AAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTT
                     ILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHW
                     GDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLR
                     DSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIP
                     KGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNG
                     LAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDR
                     SFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHIN
                     LTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          693 a          823 c          907 g          753 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggt
//
